LOCUS       DACDKL010000001        56017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDKL010000001 DACDKL010000000
VERSION     DACDKL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.001"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..886
                     /locus_tag="IV329_000001"
     CDS             29..886
                     /locus_tag="IV329_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8939214.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1001..1921
                     /gene="pstC"
                     /locus_tag="IV329_000002"
     CDS             1001..1921
                     /gene="pstC"
                     /locus_tag="IV329_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8939215.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1921..2805
                     /gene="pstA"
                     /locus_tag="IV329_000003"
     CDS             1921..2805
                     /gene="pstA"
                     /locus_tag="IV329_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8939216.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2820..3626
                     /gene="pstB"
                     /locus_tag="IV329_000004"
     CDS             2820..3626
                     /gene="pstB"
                     /locus_tag="IV329_000004"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8939217.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3642..4400
                     /locus_tag="IV329_000005"
     CDS             3642..4400
                     /locus_tag="IV329_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8939218.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4413..5090
                     /gene="phoU"
                     /locus_tag="IV329_000006"
     CDS             4413..5090
                     /gene="phoU"
                     /locus_tag="IV329_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8939219.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5312..6346
                     /locus_tag="IV329_000007"
     CDS             5312..6346
                     /locus_tag="IV329_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8939220.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            6343..7110
                     /locus_tag="IV329_000008"
     CDS             6343..7110
                     /locus_tag="IV329_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8939221.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            7210..8778
                     /gene="liaX"
                     /locus_tag="IV329_000009"
     CDS             7210..8778
                     /gene="liaX"
                     /locus_tag="IV329_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8939222.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            8957..9262
                     /locus_tag="IV329_000010"
     CDS             8957..9262
                     /locus_tag="IV329_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8939223.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            9262..9612
                     /locus_tag="IV329_000011"
     CDS             9262..9612
                     /locus_tag="IV329_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8939224.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            9778..10716
                     /locus_tag="IV329_000012"
     CDS             9778..10716
                     /locus_tag="IV329_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8939225.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10731..11561
                     /locus_tag="IV329_000013"
     CDS             10731..11561
                     /locus_tag="IV329_000013"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8939226.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            11605..12630
                     /locus_tag="IV329_000014"
     CDS             11605..12630
                     /locus_tag="IV329_000014"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8939227.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12645..13583
                     /gene="galU"
                     /locus_tag="IV329_000015"
     CDS             12645..13583
                     /gene="galU"
                     /locus_tag="IV329_000015"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8939228.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            13708..14157
                     /locus_tag="IV329_000016"
     CDS             13708..14157
                     /locus_tag="IV329_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8939229.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            14150..14764
                     /locus_tag="IV329_000017"
     CDS             14150..14764
                     /locus_tag="IV329_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8939230.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(14827..15930)
                     /locus_tag="IV329_000018"
     CDS             complement(14827..15930)
                     /locus_tag="IV329_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8939231.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            16140..17141
                     /gene="ccpA"
                     /locus_tag="IV329_000019"
     CDS             16140..17141
                     /gene="ccpA"
                     /locus_tag="IV329_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8939232.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            17495..17890
                     /locus_tag="IV329_000020"
     CDS             17495..17890
                     /locus_tag="IV329_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939233.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            17887..19143
                     /locus_tag="IV329_000021"
     CDS             17887..19143
                     /locus_tag="IV329_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP8939234.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            19155..19478
                     /locus_tag="IV329_000022"
     CDS             19155..19478
                     /locus_tag="IV329_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939235.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            19471..19737
                     /locus_tag="IV329_000023"
     CDS             19471..19737
                     /locus_tag="IV329_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939236.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(19900..22245)
                     /locus_tag="IV329_000024"
     CDS             complement(19900..22245)
                     /locus_tag="IV329_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8939237.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            22524..23426
                     /locus_tag="IV329_000025"
     CDS             22524..23426
                     /locus_tag="IV329_000025"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8939238.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            23637..25412
                     /locus_tag="IV329_000026"
     CDS             23637..25412
                     /locus_tag="IV329_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8939239.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(25721..27307)
                     /locus_tag="IV329_000027"
     CDS             complement(25721..27307)
                     /locus_tag="IV329_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8939240.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            27558..27782
                     /locus_tag="IV329_000028"
     CDS             27558..27782
                     /locus_tag="IV329_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8939241.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27924..29675
                     /locus_tag="IV329_000029"
     CDS             27924..29675
                     /locus_tag="IV329_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8939242.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            29675..31459
                     /locus_tag="IV329_000030"
     CDS             29675..31459
                     /locus_tag="IV329_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939243.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31794..32646
                     /locus_tag="IV329_000031"
                     /pseudo
     CDS             31794..32646
                     /locus_tag="IV329_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            32743..34629
                     /locus_tag="IV329_000032"
     CDS             32743..34629
                     /locus_tag="IV329_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8939244.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34670..35572)
                     /locus_tag="IV329_000033"
     CDS             complement(34670..35572)
                     /locus_tag="IV329_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8939245.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35802..37205
                     /locus_tag="IV329_000034"
     CDS             35802..37205
                     /locus_tag="IV329_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8939246.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            37205..38638
                     /gene="ascB"
                     /locus_tag="IV329_000035"
     CDS             37205..38638
                     /gene="ascB"
                     /locus_tag="IV329_000035"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8939247.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38783..39709
                     /gene="trxB"
                     /locus_tag="IV329_000036"
     CDS             38783..39709
                     /gene="trxB"
                     /locus_tag="IV329_000036"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8939248.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39923..41650
                     /locus_tag="IV329_000037"
     CDS             39923..41650
                     /locus_tag="IV329_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8939249.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            41723..42016
                     /locus_tag="IV329_000038"
     CDS             41723..42016
                     /locus_tag="IV329_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8939250.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(42110..42970)
                     /locus_tag="IV329_000039"
     CDS             complement(42110..42970)
                     /locus_tag="IV329_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8939251.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            43062..43490
                     /locus_tag="IV329_000040"
     CDS             43062..43490
                     /locus_tag="IV329_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8939252.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43652..43828
                     /locus_tag="IV329_000041"
     CDS             43652..43828
                     /locus_tag="IV329_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8939253.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43855..44301
                     /locus_tag="IV329_000042"
     CDS             43855..44301
                     /locus_tag="IV329_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8939254.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(44457..45836)
                     /gene="brnQ"
                     /locus_tag="IV329_000043"
     CDS             complement(44457..45836)
                     /gene="brnQ"
                     /locus_tag="IV329_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8939255.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            46542..47513
                     /locus_tag="IV329_000044"
     CDS             46542..47513
                     /locus_tag="IV329_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8939256.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            47537..49729
                     /locus_tag="IV329_000045"
     CDS             47537..49729
                     /locus_tag="IV329_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8939257.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            49746..50222
                     /gene="ybeY"
                     /locus_tag="IV329_000046"
     CDS             49746..50222
                     /gene="ybeY"
                     /locus_tag="IV329_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8939258.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            50200..50601
                     /locus_tag="IV329_000047"
     CDS             50200..50601
                     /locus_tag="IV329_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8939259.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            50616..51515
                     /locus_tag="IV329_000048"
     CDS             50616..51515
                     /locus_tag="IV329_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8939260.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51658..52470
                     /gene="recO"
                     /locus_tag="IV329_000049"
     CDS             51658..52470
                     /gene="recO"
                     /locus_tag="IV329_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8939261.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            52856..53773
                     /gene="glyQ"
                     /locus_tag="IV329_000050"
     CDS             52856..53773
                     /gene="glyQ"
                     /locus_tag="IV329_000050"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8939262.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            53775..55850
                     /locus_tag="IV329_000051"
     CDS             53775..55850
                     /locus_tag="IV329_000051"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8939263.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        18262 a        10000 c        11987 g        15768 t
ORIGIN      
        1 catactttag gattaaagga gatttctaat gaaaaaacta aaattattct taccaataat
       61 aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac
      121 tgcagttggc tcttctgcct tacagccttt agtagaaacg gttgctgaaa cctaccaggc
      181 agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg ggttgtctca
      241 agtacagtca ggagcagtcg atatcggaaa ttctgatttg ttcgcggaag aaaaatcagg
      301 gattgatgct tcggcattag tcgatcatcg agtagcagtt gttggaatca ctcctattgt
      361 gaacaaggat attggcgtaa aagatatcag tatggaaaac ctgaaaaaat tatttacggg
      421 cgagatcaaa aactggagtg aagtcggtgg gaaagatcag ccagtagtta tattgaaccg
      481 tgcatcagga agcgggaccc gtagtacgtt tgaaaaatgg gtattggacg gagaaacagc
      541 tgttcaggca caagaacaag attcaagcgg aatggtgcgc caaatcgttg cggacacacc
      601 cggagcaatc agctatgttg ctttttctta cgtttccgat gatgtggata cgttgaacgt
      661 taacggtgtg gaaccaacag atgaaaatgt gacaacgaac aaatggacga tttgggcata
      721 tgagcatatg tacacaagag accatccaaa aaaactgaca aaagaatttt tagactatat
      781 cttatctgat gaagtacaag ataatattgt cggagagcta ggctatatcc ctgtttcaca
      841 tatgaaagtt gaacgtgatt ggcaaggaaa cattgttaaa gagtaatctt tacacaaaat
      901 ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca
      961 ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt
     1021 attattaaca aaatcaaaac gttcaaaaat ggaacagcgc ggaaaattca ttagtttttt
     1081 gtgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtcg ctagtaaagg
     1141 attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg
     1201 gaatccaagc caaataggag caaatgggaa acccatggta ggggcattgc ctatgatcac
     1261 aggttcattt attgtgacct ttttatctgc tattgttgcc acaccatttg ctattggagc
     1321 agcagtgttc atgacggaga tttctcctaa aaacggaacg aagatcttgc aaccggtcat
     1381 cgagctactt gtaggtattc cgtctgtcgt ttacggattt atcggtttat ctgttatcgt
     1441 gccttttgtc agaagtatct ttggcgggac agggttcggt atcttagctg gtacatttgt
     1501 gttattcgtt atgatcttac cgactgtcac aacgatgact gtggatgcat taaaggcagt
     1561 gccgcgacac tatcgggaag cttcattagc gctaggtgcc acaagatggc aaacaatcta
     1621 caaagtggtg ctgcgagcag cagtgcccgg aattttaaca gcagtcgttt ttggtatggc
     1681 tcgtgcgttt ggcgaagcct tagctatcca aatggttatt ggtaatgcgg cattaatgcc
     1741 aacaggactt actactcctg cttctacatt gacgtccatc ttgacaatgg gaatcggaaa
     1801 tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt
     1861 gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata
     1921 atgaacgcaa aacgctcaga taaaattgct acaggtgttc tatatgtggt ttcagggatc
     1981 atcgtattga tcttagcagc tttattactt tatatcttag ttcgaggaat accgcacatt
     2041 tcatggtcat tcttgactga accttccaaa gcctaccaag aaggcggtgg gatcgggatt
     2101 caattattta actcgattta tttgcttttg attaccatga tcatcagttt tcctatctct
     2161 ttaggtgcgg gtatctacct ttcggaatat gccggaaaaa actggctgac agacgtgatc
     2221 cgtacgtcta tcgaaatctt gagctctttg ccttctgttg ttgtcggttt attcggtttc
     2281 ttggttttcg ttattcaatt ccagtatggt ttctcaatca tttctggtgc attagctttg
     2341 accgtattca acttgcctct tttgactcga acggtcgagg aatctttaca agcggtccat
     2401 tacacacaac gagaagctgg attggcgtta ggtttgtcac gctgggaaac agtgataaaa
     2461 gtggttgttc cagaagcgac accaggaatt ttgactggtg tgatcctaag ttcaggaaga
     2521 atttttggtg aagctgccgc attgatttat acagctggtc aaagcgcgcc agcattagat
     2581 tttacgaact ggaatccatt aagtgtttct agtccgatca gcattttccg ccaagctgaa
     2641 acactggctg tccatatttg gaaaatcaac acagaaggta cgatgcctga tggtgtttct
     2701 gtttcggctg gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct
     2761 cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagtaaaa
     2821 tgaaaaaata taattggaat gaaacaaatg tcattaccat gccaaaagat aaggaagttg
     2881 cattatatac gaatgatctg catgtttggt atggcgacaa tgaagcaatc aagggagtgg
     2941 atctagaatt cgaaaaaaat aagatcactg cattgattgg tccctctggc tgcggaaaat
     3001 ccacgtatct tcgttctttg aatcgtatga atgatgggat tgctaatacg aaaattactg
     3061 gaaagattat gtataaagac gtggatatca atgcgcctca agtggatgtt tatgaaatgc
     3121 gtaaacggat cggcatggtg ttccaacgcc ccaacccttt tagcaaatcg atttatgaca
     3181 atataacttt tgctttgaaa cagcatggtg aaaaaaacaa aaagaaatta gatgaaatcg
     3241 ttgaaacaag cttgaaacaa gcagctttgt gggatcaagt aaaagatagc ttggataaaa
     3301 gtgccttggc tttatctggc ggacaacagc aacgtttgtg tatcgctcgt gcaatcgcga
     3361 tgaaacccga tatcttgctg ttagacgagc cggcaagtgc cttggatcct atttcgacag
     3421 gaacggttga agaaacactt gtaaacttga aagaagacta cacgatcatt atcgtgacgc
     3481 ataatatgca acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag
     3541 taatggaata tgacaaaaca aacaagatat ttaccagacc aaaaattcag gcaaccgaag
     3601 attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattatct
     3661 catccaaaga tcttcatctg tattatggaa agaaagaagc actaaaggga attgatctaa
     3721 gtttcaatca aggtgaaatc acagcgatga tcggtccttc tggttgcggg aaatctacct
     3781 atctacgttc gttgaatcga atgaacgatc tgattccaaa tgtaacgatc accggaagtg
     3841 ttttatataa agacaaagac atctatggtc cgaaaacaga tgttgttgaa ctaagaaaag
     3901 aaatcggcat ggtgttccaa cagccaaatc catttccttt ttctatttat gaaaatgtca
     3961 tctatggatt gaagttaaaa ggtgaaaaag ataaaaaagt ccttgatcaa gtagtggaag
     4021 aaagtctgaa ggcagcgtcc gtttgggagg atgtgaaaga taaactgcat aaaagtgcct
     4081 tgtctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagtgaatc
     4141 ccgaaatcat cttattggac gaaccaacaa gtgcacttga ccctgtttct actgggaaaa
     4201 tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata
     4261 tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg
     4321 aatacaacga tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata
     4381 tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat
     4441 tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc gctgtgcaca
     4501 agtcagttcg tgcatacatc aaacacgata aaatcttagc tcaagaagtt atcgaaaacg
     4561 atataaatat caataatatg gaaacgcgtt tggagaagaa aagttttgaa atgattgcat
     4621 tacaacagcc agttacaact gacttgcgta tgattatcac ggtcatgaaa gctagctctg
     4681 atttagaacg tatggccgat catgcggtgt ccgttgctaa atccacaatc cgcgtaaaag
     4741 gacagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat tacgtgaaaa
     4801 aaatggtgga caacgtccta gttgcgtatg tcaaaacaga cgaaaaagat gcgcgtacaa
     4861 tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaaccatca
     4921 aaaacatgca agaaaatcct gaaacagtca tcagtggcac agactatttg aatgttgcca
     4981 gctatctaga acgaatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg
     5041 ctacagggaa aatcactgaa ttaaatacga accacaatga gattttctaa actgaagttg
     5101 tgattctgct gtttatttag gaaaagaggt tgtgaaatca gcgttttgtc ctgagtatta
     5161 gagaaacaaa cggtaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaagg
     5221 tcagctgatt gaacaccgtt tattctggaa aagggtttgt gacaaaacat gtcacaaacc
     5281 cttttcatta ttccaataga aaggataaag aatgcaaaaa aagattagca tcatcatcac
     5341 tctatttttt atttgtatac ttgcttcatg gtttattcag cgtcaagctg ctctatatga
     5401 cgtacctgtc atcgagatcg aaagagtgag aatagaagag gatcaacaat tgattacagg
     5461 aagattgatg aatacggacc aaaaaggaga agtgatttct ttaacggctc cttatcaaaa
     5521 aaatcaaatc gaaacagcaa atataacaac aagacaaatc tatcttgtcc aaatgcatgg
     5581 agatgaatgg caactagtag caaaaaaaaa tgatggctgg ctttttttct ttacaagtgt
     5641 cttcattggc accatgttgc ttattggtgg aaaacagggc gtatttgctt taggaagtct
     5701 catcatcaac atcgttttac ttttactttt gttgctcctc tttaccaaaa cagaaggtat
     5761 ttctctctta aatatagcaa tcttatttgt cgttataggg ataatcgtta gtatattggc
     5821 gttagacgga tggaatagaa gcagtataat caaaatttcg gctgcagtta caagtgtttt
     5881 tttagcattt tttatttgtc ttttgacaat gaatcattgg aaagatcaag gactgcgcta
     5941 tgaagaactg aattatctga cacgaccgta tcgttctgtt tttctttcca gcgtgctgat
     6001 tgggacagct ggagggacac tcgataccgt gatcacagtc attgcgacat tagaagaatt
     6061 gaccaagcaa caaccgaaaa tatcagcgaa acagttatgg ctttcaggaa ggcaagtggg
     6121 ccgagatgtg attggttcga tggctaatat ccttttattc gtctatatca gcggagcgat
     6181 tccatcgctt ctgttatatt taggaaatca atggtctttc aaagaaacgg tagaacgaca
     6241 tttatcatta gaacttttac gcgtcattgc tggcagctta ggaatagtat tatcaatccc
     6301 catcgcggtc ctcttttttc tcgctgtaag gaggttaaaa aaatgaatgt acttattata
     6361 cttgttggga tttttgcaat cagtgtgcta tttgtcggtg gtacgcaagg tatgtatatt
     6421 cttttaggat tattcattaa tttaggaatc tttttcctat tattattcgg ctatcatcag
     6481 aagtggccga tattggtatt gtcgattata gggttcctat tgatcgctgt ggttatctta
     6541 ttttttatca atggttataa tctgaaaatg cgagccgctt ttgccagtat actgatcttt
     6601 ttgttttgtt tcctactatt gattcctatc actgattttt tagcgatcca aggctttact
     6661 tcgatagaac tagaagaact atctggctta gataagacgt tagcaattga ttttagacta
     6721 cttacgaggt cgttgcttct gatcagtctt agcggagctg ttttagatgc gagtgtagcg
     6781 atttctagtg gaacattcga agtatatcag gcaaatccgc aaatgtcttt taaccaattg
     6841 cgccatgcta gctttgccat tgctaaaaaa atattggcat caacagtgat gacgcttctt
     6901 tttgcgttta tggggagttc tttagcgttg attctttggt ttattgactt agacatttct
     6961 tttcagcaaa taatcaatga aaaatcattt gtcttggaat atacgatggc tattcttacc
     7021 acactggccg cactacttgt cctgccctta acttgcgtga ctacagcata tctctttaca
     7081 aaaaagaaag aacatctcaa gatggagtaa aagttacaac ttaagtccta tgacattttt
     7141 actgaatcag tgcataatat ggatgtaagg aaaagcttgc aaacaatttt gcttaaggag
     7201 gcaatttcca tgaaagaaag agaacgtatt ttagacttag tgaaaaaagg aatcctttcg
     7261 actgaagaag gcttagattt attagaaagc atggcaactg aaaaagacga aaaacaaatc
     7321 aaaaaagaag cagacaaggt aactgctagt cataaagaaa aagatcaggc aagtcaattg
     7381 atcgatgaac ttgaaaacgg tgaagaaaaa atttctgagc cagttgatcc aaaagaaaaa
     7441 gaaagacaag atcaagaaaa cttagaaaag atcttagatg aactagcaac tgaagcgaat
     7501 aaaacttcgg cgcgtctaga cgaagtaaat gctcaattcg ctgacaataa ggcagcacgt
     7561 aatgagaaac aggaagcatt gatgcagctg aatacaaaag aagaattagg cgagttgaca
     7621 gaagaagaat tagcacagcg tcaaacgtta gaagcagaaa tcaaagagtt agaagcaacc
     7681 ggcgatacac tattagaaga acaattgaag ctggaagcag aattaaaaga tatccgcaag
     7741 aatcaatggt ctgagaaaaa agaaacattt acggataagt ttgaattgcc agatgattgg
     7801 aaagatcaag cgacagatac gctgaatcaa gtaggcgaaa aaatgtcaga agctggatca
     7861 cagttaggca aattcttgaa gaaaacattc caaactgttt cagaaacagt aaatgataac
     7921 atggaatgga aagatgtcag cttacgcgtc cctggaattg cgacaacgaa atttgaacac
     7981 gaattttatt atgaagctcc tgcagcaagt atccttgata tcaaggcagc aaacggaaac
     8041 gtgacgttaa aaacgtggga cagtgatgat gtgaaagtgg aagccaagat caaactttac
     8101 ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac ggagccagat cgaggtcaat
     8161 gaagatcata tctccttcca aattccaaat aagcgtgtgc gtgcagacct tgttttctac
     8221 ttgcctaaac gagtatacga ccatgcagct atcaaacttc taaatggcaa catcatgatt
     8281 gagacattgg aagctaaaga tatttataca aaatctacga atgggaacat catcgtgaac
     8341 caattaactg caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga tattcgaaac
     8401 gggaacatct tagattctat tatagaaaca gtaaacggta cagtgacatt tggtgccaca
     8461 ccagaaaacc tttctgtgtc tttagtaaat ggtgatgtac gtcttacgat taaagaagat
     8521 aatttgaaaa aagtagaagc aagttcagtg aacgggaatg tcaaagttgc tttaccagat
     8581 agcatcggtt tggaaggaca cgctaaaaca agtcttggaa gcattaacag ccgtctgtcc
     8641 aattacgaag tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca attccgtcgt
     8701 gtttcagatg atgagatcgc acaagtgcag ctttcaacga caacaggaag tatctattta
     8761 aaagatacag ataaataaag aggttgtgaa agagatgttc agccttgagc aataagaagc
     8821 gattttttaa aatagcttcc cctattttaa aaaaatgtgc cttattgccg ataggctatc
     8881 tcttgaacac cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga
     8941 acagaggtga gacatgatga aaaaaaagtt gacaaaatcc aataaaaatg ttgttttgac
     9001 aggtacttta gcagggattg gggaatatat cgggattgat cccactgtta tccgtgtgct
     9061 ttatgtattc ttaagcttag ttgcttttgg aagtccaatc attttataca ttttattagc
     9121 tttgattatc ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta
     9181 tgaaaaaaac aattatcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga
     9241 tgacgaatgg agtgactttt aatgtcgtac tttcagcgta ttttggttaa tacgttaaca
     9301 ttcatatcgt tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc
     9361 gtccttgcca gcttcgtatt atctgtgttg aacatgtttg tcaaaccgat tttgacgatc
     9421 ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg
     9481 attcttaaaa tgacttcctt ttttgtagga gcagaacatt ttagtttttc ttcatttggt
     9541 gcagcgttat tcgtagctat cttgatgtca cttgtcaatg taattgttag tgaacataac
     9601 agcagaaaat aaaaaaagct gtgccgagat ctcggcgcag cttttttgtt tagaataaag
     9661 aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt ggtagtactt
     9721 ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt agcatgaatg
     9781 tcagaagttg taaaagttag ccaattagta aaaaaattat ctttggaaat cgtcacaggc
     9841 gacgagaaaa gtttagaccg cgtcatcgtg actggggaca tctcacgccc aggactagag
     9901 ttgaccggtt attttaatta ttattcccat aatcgtttac agctatttgg cagtaaagaa
     9961 atcagttttg cggaacggat gatcccagaa gagcgtttga tcattatgag aagattatgt
    10021 agtgaagata tgcccgcatt tattatttca agaggattgc cggctccgga agaaatgatc
    10081 caagcagcca atgaaaatgg gatagctgtg ctgcgttcgc ctatctcaac gtcccggctg
    10141 ctaggtgaat tatctagtta tttagatgga cgcttagccc ctcgaacaag tgtccacggt
    10201 gtgttagtcg atgtctacgg attaggtgta ctaattcaag gggacagtgg gatagggaaa
    10261 agtgaaacgg cgttggaact gattaagcgc ggacatcgat tgatcgcaga cgatcgagta
    10321 gatgtctatc agcaagatga actgacggta atcggagaac ctcctaaaat cctggaacat
    10381 ctgattgaga ttcgagggat cggtatcata gatgtaatga atcttttcgg tgcgagtgcg
    10441 gtacgaggct ctatgcaagt ccagttagct gtttatctag aagcatgggc gaaagataag
    10501 aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt gggcattcca
    10561 caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga agtagccgca
    10621 atgaatttcc gcgcacgtac aatgggcttt gacgcaacga aaacatttga agaaagatta
    10681 agtcgcttga ttgaagaaaa ctcaggcaat gactagcaag gagtgacaat atgttagcac
    10741 aaatcaatcg agttgctttt gagcttgtag gaattcctat ctactggtat gcattgatta
    10801 tcgtatcagg aatcattatt gccatgtggc tcagcagcag agaagctgtt cgggtaggat
    10861 taaaagcaga tgacgtgaca gattttatgt tgtggggatt gccgatttcc attattggtg
    10921 cccgcttgta ttatgtttta ttcgatcttc cccaatatat agctgatcca atccagatat
    10981 tcaatatccg ttcaggagga ttagcgattt acgggggatt gattgcaggt gcaatcacac
    11041 tgtacttttt taccaaatat cattttattt ctttgtggac ctttttagat atcgctgcac
    11101 caagcgtatt attggcgcag gcaatcggac gatggggaaa tttcatgaac cacgaagcgt
    11161 atggtccaga cacgacacga agtttcttag aaagtctgca tttaccgcaa ttcatcattg
    11221 ataacatgta tatcgaagga atctatcgac aaccaacctt tctttatgaa tcactttgga
    11281 gtttgattgg atttattctg cttttggttt tgcggaagaa accacatctt ttgaaaaaag
    11341 gagaagtcct tttatgttat ctaatgtggt atagtttcgg acgtttcttt atcgaagggt
    11401 tacgcacaga cagcctttac tttttaggtg tgatccgcat ttctcaatta ttgtctgctg
    11461 ttttatttgt cggcacattg attctgttta tttggcgtag aaaaaaagga agtttaccgg
    11521 attatgaccg ctcattagga aaaaatcaac aaattatttg attttcagag cgttaaaaat
    11581 gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg accaggctct
    11641 tgggggactg ctttagcaca agtcctcgct gaaaacggac atgacgttcg gatttgggga
    11701 catcgtgctt cacaagtaga agaaatcaac acccaacaca ccaatcaacg ttatttacca
    11761 gatcatcatc tacccgattc catccgagca ttcaccgata tggaagatgc agtaaaagat
    11821 gcagatgcgg tactattcgt tatcccgacg aaagcgatac gttctgttgc aaaagaacta
    11881 gttgccaaat tgaatacgaa gccagtgatc atccacgcaa gtaagggatt agaacaggga
    11941 acgcataaaa gaatctctga aatcttgtta gaggaaatcc cgacagaaaa aagaaaagct
    12001 gttgctgttc tttctgggcc aagccatgcg gaagaagtag caaagcatga cattacgacg
    12061 atcacagcgg caagcacaaa tgtaaaagca gcaaaatatg tacaagagct gttcatgaac
    12121 cattattttc gaatctatac caaccctgat gtgattggtg tagagatggg agcagctttg
    12181 aaaaatatta ttgccattgg agctggtgct attcacggct tagggtttgg cgataatgca
    12241 aaagcggcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt agcaatgggt
    12301 gccaacccgc tgacctttat tggcttaagc ggagtaggtg atctagtcgt tacttgcaca
    12361 agtgttcatt ccagaaactg gcgcgcaggc aacttgttag gacaaggtca gcctctagaa
    12421 gaggtgctag ccaatatggg aatggttgtt gaaggtgtga atactactca agcagcgatg
    12481 gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta tgaagtgctg
    12541 tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga tgggaaaaaa
    12601 gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa gtaaaaaaag
    12661 cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaag gcgatggcaa
    12721 aggaaatgtt gccaatcgtg gacaaaccaa cgatccaatt catcgttgaa gaagcgttga
    12781 aatcaggaat cgaagacatc ttgatcgtta caggaaaagc taaacgacca atcgaagatc
    12841 actttgattc aaatatcgaa ttagaaacaa acctaagtga aaaaggaaaa accgaattat
    12901 tgaaattagt agaagaaacg acagatgtca atttgcattt catccgtcaa tctcatccaa
    12961 aggggttagg acacgccgtt cttcaagcaa gagcctttat tggaaatgaa ccgtttgtcg
    13021 ttatgctagg cgatgatatc atggaagacg aagtaccatt gacacagcaa ttgatcgatg
    13081 actatgagcg tacacacgct tctacgattg ccgttatgca agttcctcac gatgaaactt
    13141 caaaatacgg aatcatcaac ccaggggaag tactggaaga tgggttatac aatgtcaaaa
    13201 actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt attgggcgtt
    13261 acttattgac tccagaaatc tttgatgtat tatcagaaca acaaccaggc gctggaaatg
    13321 agatccaact aactgacgca atcgatacgt tgaataaaac acaacgcgta tttgcccgta
    13381 aattcacagg taaacgttat gacgttggtg acaaatttgg ttttatgaaa acaagtattg
    13441 aatatggtct gacacatcca gaagtagggg cacctttgcg tgaatacatc atgtctttag
    13501 gtaaaacatt agaagcagaa ggaagcaaga tcaatcataa tccaaaagga ataacagaaa
    13561 tcaaagaaga aaagaaaaaa taatcttttt ttctaacgag cttcagctgc taattgaata
    13621 aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta tccttataga
    13681 aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg attgatcgca
    13741 gcaatcgcct ttgcgatact tgtcgttttc atcgttcgcg tattgttgca agtttccaaa
    13801 actttagaaa aagtagacca gactgtcgca gaagcaaata cgacaattga gatcgtcaca
    13861 caagacgtag atcttttatc tagacaagta gaaggcttgc tagtcaaaag taatgagctg
    13921 ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc agttgctgat
    13981 ttaagtgaaa gtgtatcgga acttaatcat tctagtaaaa acatcgtgac gaaagtcggc
    14041 ggaatcggaa aaacgacagc taaagcgact gttgccggaa aagtcggtgg aacagcgatg
    14101 aaatttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataagaa tggctaaaaa
    14161 aggtggattt ttattaggtg cagtagttgg aggtacagcc gcagcaatcg ccgcattatt
    14221 gttagcaccc aaatctggta aagaacttcg ggatgaattg gctgctcaag cagatgattt
    14281 gatggacatg gcttcagatt acacagatat ggcaaaagaa aaatcatctg agttaacagg
    14341 tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggggatctag caggcaatgt
    14401 taagcagaag gtaagtgaat cagcagagga agcaggaagc gaatcagatg atatcttgtt
    14461 aaacctcaaa gaacaatcag ctgatttatc taatcgtttc aaaaaaacag cagaagatct
    14521 gaaaaagcca gcagacgaat taggtagtat tgctaaagat acttcagaag atatcatgat
    14581 cgatgtaaaa gatgctgcag ataaagcagg agagacggtc aatgaaggaa tgtcagaagc
    14641 aaaaccagtt gcagatgaca caaaagaaat gttgaaaatg gcaaaagaag atgcaaaaga
    14701 agatgcaaaa gaagctaaaa aagaaatgaa agatacaatg acagattcga ctaccaatca
    14761 ctaagattac tcatttctag aataaaaaag gtgagagcgg accagctctc acctttttta
    14821 ttttcattac tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacaaccatc
    14881 acttgtcaca tacacacaat cttcgatacg tacccctact ttttcaggaa tatagattcc
    14941 tggttcaaga gagaagcaca tcccttcttc aatcactaag tcgtttcctt ctacaagaga
    15001 agggaattcg tggacggttg ttccgattcc gtgacctaaa cggtgattga aatattcgcc
    15061 gtagccatag ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc
    15121 tgggcgaact gcctcttgtg cggcaagctg tgcttcaaga gtgatgttat agattttttc
    15181 ttggaattca gttggttttt gataagcaac tgtacgagta gcatcactgc aatagccatt
    15241 ccaaataacg ccaagatcga ataacactag ttcatttggt gaaaccttcg tgcttcctgg
    15301 tgtaccatga gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg
    15361 tgaaacacct tgttttttta attggtattc gatttctgcg ataattgctt gttcagttac
    15421 gccttctttt actgttttga agccaatttc aaaggcaaca tcggcccatt taccagcttc
    15481 gatcagtttg tctttttctt ctaccgtttt gatcagttgc agacgctgga taatagaagt
    15541 tagatcggtg taagaatctg tacctgtcaa gatatcagag atagcatcga agcgatcgac
    15601 agtcagagca ttcttttcaa tacctacatt accaggttgc ccatagcgtg cgcgaagctc
    15661 ttttccgatg atctcccaag gattttcgct gtctaagtag ccacgtacat cgtacgccca
    15721 gctgctgttt tgagcatctt caacttccag agcaggagtg aaaaggaaag gatcttgttc
    15781 cagagggata aaaagagcta gtacacgctc gtgaggatca ctttcatatc cggaaaagta
    15841 agcgatgtgg ccagggtcgc tgatataagc caattctaat tggtgttgtg ccatccattg
    15901 acgtagttca ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt
    15961 attcaataat tgtaccactg attgattaaa agaccaacgt tcttaaaact gaaaaaagta
    16021 aaaattttca tgaaaacaga gataaaacgg ttgcaattgt tgagattatt tgatatccta
    16081 ttttagaaat gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa
    16141 tggaaaagca aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta
    16201 ctgtttcacg tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct
    16261 tggaagttat cgatcgcttg gattatcgtc caaatgcagt cgcacgtggc ttggcaagta
    16321 agaaaacaac aacagttggg gtaatcattc ctgatgtcag caatatgttc tttgcctctt
    16381 tagcacgtgg tatcgacgat gtggcaacaa tgtacaaata caatatcatc ttagcaaact
    16441 cagatggcaa cgatcaaaaa gaagtcaatg tattgaataa tttattggct aagcaagtgg
    16501 acggcgtgat ctttatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt
    16561 caaaaacgcc tgttgtacta gctggttcca ttgatccaga tgaacaagta ggtagcgtaa
    16621 acattgatta tgtatcagca acaaaagatg cagtaaataa attagcaaaa agcggcaata
    16681 aaaaaatcgc ttttgtcagc ggtgcgctga ttgatcctat caatggtcaa aaccgtctaa
    16741 aaggctataa agaagcatta agcgaaaaca atctaccata tagcgaagga ttgatttttg
    16801 aggcacaata caactttaaa gatggtctgt ctctagttga ccggatccat aatagcggtg
    16861 caacagctgt ctatgtttca gacgatgaat tagccattgg tatcttggat ggtttattag
    16921 accgcggcgt aaaagtacca gaagaattcg aaatcatcac aagcaacaat tctttactga
    16981 cagaagtagc tcgtcctcgt ttgacaagta tcacacaacc attatacgat ttaggagcgg
    17041 tatccatgcg cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta
    17101 tcttgcctta tggaatcgaa gaaaaaggat caactaaata ataaaagatc aatcatttaa
    17161 aggttgttcc caaagtggtc aacttctgga acaaccttta tacgagaaaa ggggttatga
    17221 caaaattttg tcataacccc tttttttgtg actttaaaaa aaagagagaa tggcggaaac
    17281 aaaccttacg tccatcgtat acgacacaat ctgtgcgtag atgctacatt ttgttcacta
    17341 ctatcaaaat tactttaaaa tgaaaacgtg ataaaaaaga aagtagaaaa gtagaaaagt
    17401 aactctaaaa aatccaaaaa aatccaggct cgattgcgta cttatcttaa aggagaaaca
    17461 atccatcata cataacccga aaggagactt ctatttgcct aagaaaagac gaaagaccat
    17521 agtcatcatt ttgaccattc ttactatagg aatcggagga aaattttaca tggacaaaag
    17581 agaagcgcaa aaacagcaag atttactcgc agtagaaaaa cagtccgtca aagttctgaa
    17641 aaataccttt gcggatatca aagaagtgaa aatcgaacaa tttgagaaaa acgacatgac
    17701 aggaatctat gaagcattga taacgatgac caatacagaa ggaaaagccg tttattttga
    17761 ttatagcttt acaaaagaaa gcaatgagat agatggctat ggtgttgaaa atgaagaagt
    17821 acaaaaagaa gggattacgg ttgatgagat tatagttatc tatacagatg ggagtgagga
    17881 aaaagtatga caattactga taagcagtat aaaggaataa gtaataaagt atattggcta
    17941 gatccaaaac atcctcaata tagtaaaagt tacaaagaag gcgccattaa aaactttgga
    18001 gaaacaaaat tccaaatcct aaaaatcaaa aactccttcg acggcatgca ggcgatggct
    18061 gtagcgccaa tcaagaacgg taaagtggat atttctcaag tagtgattgc ttttgcagga
    18121 acgaacttaa gcgattggaa agatttggaa acggatgcaa gaagtgttat gaatctctat
    18181 gggaatagac caggagcaag tttttctgaa aaagcaacaa caaacaagat tttatccgtt
    18241 agtggattga aaacagcggg gcaaatcaat tcagcgattg catttgctaa tgaagtaaaa
    18301 aaggaatatg gagaagctgc tatttctact accggccatt cattaggtgg attcttagca
    18361 ttatatgtag cagcagagaa acaatggaaa aatgtcggct tcaacgggcc ggacccgtat
    18421 gatctcttat caccagaagc aaaaaaatgg gtggaagaaa atcccggtat gctcaccaat
    18481 taccgaaatc acgccgattt tatcggtaat tttggtggta atggcacagg tgctgaagtt
    18541 cggatcagta tggaaacagc atacttgaat ctgcttgctt cccacagttt aacctcttgg
    18601 aagtttgatg aaaatggaaa gctgattatt cctgaaaatg aaaacaatgt taaagctcga
    18661 aaacaacaga aagaaaacca agcgacccag ttgttttcct atcagttgca agaactgaac
    18721 aacatacgta aaaaactacg attaagtggc ggccggctga cagttaatga acaaatttac
    18781 ttagatgaca cacaagctag gttagtggtg gaaaagttat cggtaaaaat gaaaagcgct
    18841 atggaagaag ttatccgcat caaccaaaga ggaatttttg agctagagaa aaaatggcaa
    18901 gaaggcttgc ggcaagcacg tgttgtcgcc ccttcgttga ctgaacagga aatcatagaa
    18961 gcattagcta gtgtgggcgc cacaaaagag aacttagtcg attctgcgaa agaagtctat
    19021 ctgctacgaa tcaataaggc aagacgtatg ggggaggcct ttgatacatt ggcgaaggag
    19081 atccaagaca aaatcaatga aatcgtggca cgagatcgag aattagcaca gcaattcaat
    19141 taaaggagaa gtagatgaaa ctagaagaat tggaaagaga ttatcgaagt gaacgaagac
    19201 aattagaagc tcaagaagat gaattgatcc actttaagaa tcaaggatta cgattgatgg
    19261 agcaagagga gcagcgactg catcatgtat taaagaactt agctttagat aatgagcctc
    19321 tgtttcaagc aagacgaagt tattcgaatt atcaggagca ttatcaacaa gccctacaag
    19381 tagaacgacg aaaactctat cagcagcaag acgaactaga acgttcctat cgtcaaaaaa
    19441 tcagacaaat aactatggag aaatcatctt atgaatgaat ttttaatgca attactaaaa
    19501 gaattagaga aaaatctaca tcgatcaaat gtacgttcag ttggtatggg gatatctcag
    19561 caaacttggg cagaaaaacg attgacagat caaaaccacc aaatgactgc agaagaagct
    19621 actcaagaat tttctaaccg acatgcacac ttaaaagaat cagtaaaagg gcaatttgca
    19681 gaaaaactaa ccgaattatt ccaaagacaa gaaaagaaat taaaacagtt tcgttagtaa
    19741 agttatatag aaaaattttg tgaaaatggt ttttcacatt tttacataaa tttgtcttaa
    19801 tatcgtagat gtaagctttt gaacaccatt tattcggaaa aagggagctg tgacaagatt
    19861 tttgtcgcag ctccctttaa ttctgattaa tctattagat caatcatctt tatcgtctga
    19921 ttctttggtc tctggattcg ctctagcata agctgaagcg gtgttccagt aatctgtata
    19981 gttttcatca gtaccgccaa cgccgaattt aaaggcgctt ttcttcggac cattttttgc
    20041 ccaaagtgat tccactgttt ttcctggcgt ttggatagat tttttattga cagtaacttt
    20101 tccagctgtt tgtcctgtga acccagatac ttttttcttc atcacatcgg atgataagtc
    20161 aaatttctca tcagtgccga aaatctcagg gtttgcttgg taagcttgat tgattagatt
    20221 ggcaagataa ttagccgaac gtattcttgc ttggctgtcc attccagtat tgtcatcgtg
    20281 tcctgcccaa ctactgatag tgatagatgg agttgagacg atcagccatg aatctcggta
    20341 ttcattggta gaaccagttt ttccgaccca atctgcattt cctagatttc cgtttaagct
    20401 tgagatgaca tctttgaatg gtgtagtgat tttttcattg atcacactac gcatcatgtc
    20461 attcatgatc gatgccgttg cttcggaata aatacggaca gaattacttt catgtttata
    20521 aatcacatta cctgcattat ctgtgatgga atcaatgata tagccttctt cgtagactcc
    20581 accatttgct aaggcctgga atccatttgt ctgttgaagc gtcgttacat ctactgtccc
    20641 taaaggagcc gattcaacgc cccaattatc atttgctggg tagttcatct tcgccaagta
    20701 gtgctcgtaa gcgtattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata
    20761 agctgggata ttgttggacc attccagtga ctcacggacg gtttggaagg tgttcgaccc
    20821 ttcattggtc gcattgacga ttttctcacc agcatttttt ccttcttgcc aagtcgttgc
    20881 ataatctgat acacgggatt ctgatccgac aagtcccata tcgattgcag gaccgtaaac
    20941 aagaaccggt ttgatggatg aaccagcttg acgttctgta tcaaaggcgt ggttattttg
    21001 attctggctg taattccgac cacccacgaa gccatagata cgtccggttc tgttatccat
    21061 caatacattt ccagtctcca cttggctacc agcaccatca tctaacatgt agccataatt
    21121 ttctacgccg gtttgcaatg cagcatagat atctttatca atcgtcgaat ggatcgtata
    21181 tcctttgttt tggatcgttt gctgtgcttt ttgataataa gcttcactga ccgatgaatc
    21241 tgacatatca gcattatctt tttctgccaa ttgttgtgca ataatccttg ttgcttcgtt
    21301 catcacggta taatacaaga attctctgtc agtctgttcc gcgatttgtt gtgggagaaa
    21361 gtcttttgta agatcatagg ctttcgcttc ttcgtattgt tctttcgtaa tttgtccttc
    21421 acggtacata ctgaataaaa caaaatcttt tctggcaaga ccggcagaaa gatcttcttt
    21481 tagcgcgcct gtattcgtgt atggactata cgtgataggg ctttgaggca aaccagcgat
    21541 ataagcagct tgcggcagag tcacatcctt tgcatctaca ccaaagattc ctctagcagc
    21601 ttcctggact ccagcaatat tttgcccctt attattccga ccaaatggtg agacattcaa
    21661 atacgtagta atgatctcat cttttgaaaa gtgtttttct acttgtgcag aaagaacgat
    21721 ctcgtttgct ttacgtttga atgttgtttc atcggttagg atctgttgtt tgactaattg
    21781 ctgtgtcaaa gtggatcctc cagacgagcc gatgccagtc gcttctgaaa ctagtgctcg
    21841 taaaacagct ttagggacat aaccttggtg tttatcgaag tattcatctt ctgtactgat
    21901 aatcgcggtt ttcaataaag gcgaaatctt atcagatgaa atagtcgtcc gcatcagatc
    21961 tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg tttccgtaat
    22021 gtttcctagt tctgtctgca gttctttttt agtcggcaat tgtgtgtctt cgacaagata
    22081 ggcaaagtag ccagcaccta ttccgatacc tagagctgcg aacaacaaaa gaagaacagt
    22141 aataaaaaca gtcaatgatt gaaacactct aaaaataatc ttaggaacaa accatttttc
    22201 tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac
    22261 taatggtttt atttcgctaa ttatagcaaa taataggaca aaaaaacaga caaggctgtt
    22321 tttgacagga atttcttttt cgaatgagat aaaacgtgag gaaatgatct tttttatgaa
    22381 agagttctta agcttttctt tagggaaagt ttcagttact atggagaaca gggcaaagaa
    22441 aaacaaacta ttttctttgt acatcatctt atcaatatac aggaattgtt cgaaacaatt
    22501 gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga
    22561 aaatgctgtt aggcgcggca gaagaagcag ccagttacgg atcttcgacc tttatgatct
    22621 atacaggcgc tcctcaaaat actagaagaa aagctatcga agagatgaat attgatgccg
    22681 gaaaagcatt tatggcagaa aacggactaa caaatatcgt cgttcatgcg ccttatatca
    22741 ttaatttggg aaatacgatc aaaccagaaa tgtttccatt tgctgtacaa tttctgagag
    22801 aagagattgt ccgtgcggaa gctttaggtg ctactcaaat cacccttcac ccaggcgctc
    22861 atgtaggagc aggggcagaa gctgggattg ctagaattat tgaggggttg aatgaagttt
    22921 tgacaaaaga acagacggca cagatcgctt tggagacgat ggcagggaaa ggtacggaaa
    22981 tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat
    23041 tatccgttac ttttgataca tgtcatacga atgatgccgg atataatata aaagaagatt
    23101 tttcaggtgt aatggaagag tttgataagg tcatcggatt agatcggctt aaagtactgc
    23161 atatcaatga ttctaaaaat cctcaaggtt ctcataaaga tcggcatgcc aatatcggtt
    23221 ttggaacaat tggatttgac acattgaaca aaatcgtcca tgatccaatg tttgacaaca
    23281 tttcgaaaat attagaaacc ccatatgtcg gtaaagataa aaagaaagct tacgcaccat
    23341 atggaaaaga aatttccatg ttcaaagcgc aagcattcga tcccgaagtg ttccctgagt
    23401 tattagcaga agaagtcaca ctttaagttt tctacctgac agcaacaaaa aaggattggg
    23461 cagtctgcct tgtccttttt tgtttaagaa acaaaggagt gctttttgca ttcgttttcg
    23521 ccatgctcct tttgccccct ctttttaaat tcagacaaac agggtatact gatagaaaag
    23581 taaaaaacga aaagtttcac aaacaagtag caaaacgtga ttgaaaggac gaaaagatgc
    23641 tgaacatttt aaaccccttg atcgaccaac taaaaaagaa acatacatac tttattcttg
    23701 gcatagccgt attatttaca attattcggt tgtatttata tagtaaagca atctatggaa
    23761 cagatatgtc ggcgacctat gatgatcagc tgctgattca ctatgctcaa aatattttgg
    23821 atggtaaatg gctaggggag tacacgacta cgacactttc aaaagggatt tcctacagct
    23881 tgtttttagt acttgcacat aaactgcatc tttcttatgc agttttattc ggcatgttca
    23941 gtattcttgc cagtatattg gttgcactag cgtttaaacc aatcgtgaag aacaagtatc
    24001 ttcttttaag tatctatttt tattttttgt tttctccgat tagttttaca catgaatatt
    24061 ccactcgaac atatcgtaat gctctcgtga tcccgactgt tgagatcatt atcgcttgtt
    24121 tactagctat ttattaccgc aaatataatt ccagcaaaaa attgctgccg tggtttttag
    24181 ggctagctgt ttcttttcct ttcttttggt tcattcgcga agattccatc tggcttcttc
    24241 cttttactgt agtcgcgtta ggcatcagtt tcgttcagat cttgctagga caagactttt
    24301 ctttcaaaga agcaaagaag ttaaaagaac gaccatttct cctttcgaaa caagaatggc
    24361 tgaaactttc attgttttta ctccctctag tcggtttctc catcgtctat cagtggatcg
    24421 aatataaaaa cgagacaaca tatggtattg cagccatcaa tgaccgttcg agtggcgaat
    24481 ttggacgttt gatgaaacag ctgatccgaa tagatgacga aacaaactta aataaagaaa
    24541 acagtgagat atgggtctcg catgctgcat taaataaagc tttagacgca tctcctacat
    24601 ttcatactgt ttctactcgt attgacgaat tgtatacgac tcatgcgtgg acccaaggcg
    24661 gaaaagtaaa agaattacca ggagatatca ttttttgggc attgcgagat gtggtaagcg
    24721 aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa aaagtcaatc
    24781 aagaattaga agaagcctat caaaagggaa ctatcaaaaa gaaaaaagaa ttctatctga
    24841 tgggatcagg ggatggaaag acgatgaatg atctgccaat cgtatttgat tttctgaaat
    24901 caggatatgc ttatgcatgg cgatacaaag atttccatca aggggggaat tatagttttg
    24961 ggacgcaaga acaagtcgaa caggcacaag aattattgaa tcttccacta atggataatt
    25021 ggatcgatag cagtgatccg agaacacctg atttccagtt gacaaaaaca gcaaaagtcg
    25081 cgaatgtcat gataaaaatt taccaagttc tggccattcc cgtgctgatt gcttcaatag
    25141 cagggatatt acttatgata ctaggaaccg tatttgcaaa agctgatcgt gcctattata
    25201 gttcctcttt gattgtagtc atcggattaa tactgacaca attggttttt cttttcggtg
    25261 tctcgtggtt ttgttcttat gcgccagaaa aaaaggatta ttttttaagt gtctacactg
    25321 gcgcaggcgt gccattaacg caattcatcg cagtaacaag catgctagtt tttagtaaat
    25381 tgaatctgaa aaaagcttta tcttccaaat aaaaagggaa ctgtgacaaa agtcttgtcg
    25441 cagttccttt ttccgaataa acggtgctca aaagcttgca tctgcggtaa taagctcaac
    25501 caaacgtaaa atatgaggaa ctatttttgt ttggctgttc ttattacttg atgcagggca
    25561 gctttttcac aacctctgat acacggtgtt ccagaagtaa cttcttcgga aataagccga
    25621 aatttcacaa aaatttgaaa agcaattttc ttaaattcct tcttatttct tgaagctgcc
    25681 cacttctgtc acaacctcct ttgtgtaaaa gaatcagttt ctattcgatt tcttctatcg
    25741 tttcttgcaa acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    25801 ccaacaggag aaggacaaga acaatgatta cataggcgat ttgatcagcc aatgctagtt
    25861 gagctgaggt ggcttgctcg ccgaagaaac tttcgatttg acctggtaat cctcgtaagt
    25921 tcaagtaagt cagactaatc acagagaacc agccaagaag ttttacaaca aatgaatttt
    25981 tgaatcggct ccccatctcc gcttggctat tggtcatcat cagtaaagga atcatcgaga
    26041 atggcaatgc aaaagccaga aaaacttggg attcattcat caagttattc aaagcggtat
    26101 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    26161 aaagcaatcg tgtgactagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt
    26221 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    26281 gtgctacagc aaataaagta gacaagatac cagattttgc tacagcaatc aagataccat
    26341 tgctcatagc tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgga
    26401 cagaaccggt tttgaataca gcgacaccca tgatcaatag cagtgcattg actaaaaaag
    26461 ccattgacaa ctggatatta gagtcccaag tagaaaagcg taccgcttgt gctacatctt
    26521 cttcgtcttg gtgatcgatc ttccgagttt gagaaactgc tgaatgaaga tataagttat
    26581 gcggcatgac tgttgcacca ataatcccta acgctccagt cagcggagtt tcaccaccta
    26641 tgacaggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    26701 aatcagggtt agacaagcca acttgataga cgaaaacgaa taaaatgacg aaaatcaagc
    26761 agacaaccaa agcttcgatt ttacgaaacc cgacttttgt cagcaataaa aggacgaaaa
    26821 catctaacac agtgataaat acggcaatta ccaatggaat atcaaaaagt aagtagaggg
    26881 cgattgctgc accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga
    26941 tccataaaat gacacccaat gtcttacttg tccgtgctcg gattgcttgg gccaaatcca
    27001 tttgggtcac gattcctaat tttgctgcca tatactgtaa aagcatagca attagactag
    27061 acatcaaaat aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg
    27121 accagttacc tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa
    27181 gaagtgtttt aaaaaaactt tttccctttg gtacatccac tgttccattt acttcagcta
    27241 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat
    27301 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcagaaaga
    27361 aaaaattaaa gaaaaagatt cggtatgcct aatttttttt cgtggcgtgt cttgctggtt
    27421 aatgaaagaa attttgttct tctctaaaaa aaccactatt ttttgcatga aactaaagaa
    27481 tacgcctttc ttttttcaat aaactttagt aaaataaatc attgagagca attcggaaat
    27541 agaaaggaga gatttatatg gtatcaacag gtattgtcgt acttattgct attattgctt
    27601 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    27661 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atggggcaaa
    27721 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    27781 aggttctgac ccaaagactt tttgcgggct gattttttca gtccgttttt tatttcgtaa
    27841 tttgttagac tattcagact attcagcaaa aaagacaagg acagacaatc ctatgctctt
    27901 gataaagaca aaggaggagt aaaatgtcga tttttagaaa attaggttgg ttttttcgtc
    27961 aggaaaagaa acattattta attgggattt tctccctatt cgcagtggcc ttggtacagt
    28021 tagttcctcc aaaagtgatt gggatcatca ttgatgagat cgccgataag aatttatcaa
    28081 taaagatcat ttcttggtgg atcttactat tgatccttgc agctttctta caatatcttt
    28141 ttcgttatat ctggcggacg aatatttggg gaagtgcagc tcggttggag aaagaacttc
    28201 ggcagcagct attcaaacat tttacagaaa tggacaatct cttttaccaa aaatatcgga
    28261 ctggagattt gatggctcat gcaacgaatg atttgaatgc aatccagaat gtagcaggag
    28321 ctggaatctt gacctttgca gattcagtga ttacgggcgg aacaacgatc attgcgatga
    28381 ttttgtttat tgattggcgc ttaacactga ttgcgctgat tcctctgcct ttgctagccg
    28441 ttacttcacg agtgctgggg tcaaaactcc acgatgcttt tcgtgattcg caagaagctt
    28501 tttctagtat caatgataag acacaagaaa gtatcactgg gatcaaagtc attaaaacat
    28561 ttggacaaga aaaagaagat ctcgcggatt ttgaagaaaa gattgaagat gcaattatta
    28621 aaaacaaacg agtcaatttt ttagactcct tatttgatcc attcattacc ttgattatcg
    28681 gtctgtctta tgttgcaaca attattttag gtggaaagta tgtcttggaa ggcacgatct
    28741 cgattgggca gatggtctca tttattagtt atatcggtat gcttgtttgg ccgatgtttg
    28801 cgattggccg attgttcaac gtattggaaa gaggaaatgc ttcttatgat cgcgttaatg
    28861 aacttttgca tgaaaagacg catatcgtgg aacaactggg tgcagtgcag accccggcaa
    28921 aaggcgagct gaatgtagct atttcctctt ttaactatcc gaaagaagaa tcaccagcat
    28981 tagaacaaat caaatttgcg atccaagaag gagaaacatt aggtattgtt gggaaaactg
    29041 gtgcgggaaa aacgacaatc atcaaattac tgatgcgtga atacgatgat tatcaaggga
    29101 aaatccaatt tggcggacgt tcgatcaaag aatattcatt gaatgcttta ttaggatcaa
    29161 tcggttatgt gcctcaggat cacttcctgt tttcgatgac cattcgagat aatatccgtt
    29221 tttctaatcc aaaactttca gaagaagaag tagagcatgc ggcttcttta gcatttatca
    29281 acaatgaaat caaggaattg ccaaaaggtt atgatacctt agttggagaa cgtggagtct
    29341 ctttatctgg cggacagaaa caaaggattt cgattgctcg agcattgatc aaaaatcctg
    29401 aattgctgat cttagatgat gcactttctg cagtagatgc caaaacagaa gaagctatct
    29461 tgcataattt aaaagaaata agaaaagaga aaacaaccat cattgcagct caccgtttaa
    29521 gcagcgtgat gcatgcaaaa gaaatcattg ttgtcgatca agggaaaatc attgaacgag
    29581 gaacccacca cgagctgctt gcattaggtg gctggtatga acgaatgtgg gaaaaacagc
    29641 aattagaagc gaagatagaa ggaggcgagg cctgatggat gaaaattatc aatcggaatg
    29701 gacaaaatcg attccactta aagaacagaa gaagatcatc aaacgactga tgaagttcgc
    29761 caaacctttt agaagaacgt tcatcgttgc tatcttattt gcgtttgccc tgtctgtgat
    29821 caatgtccta ttgccaagga tcatccaaac ttttatggat gatcatctgg ctaaacagtc
    29881 agctacgaca caagttattt tgttttttgc tggtgtgtac ttgtttggag tcatagtcaa
    29941 aagtatcatt tggtttttcc aatggtattt gtattccatg gcttcattga agacctatca
    30001 gtatgttcgg gtaagattat ttgaaaagct gcatacacta ggtatgcgtt attttgacca
    30061 gacaccggca ggatcgaccg tttctcgagt gacgaatgac acagaaacgc tatttgaatt
    30121 ttggtatgtc tttttgatgg tattgactgg gatctttgca gtcgtttctt ctttcgttgc
    30181 catgtttcaa atcaatggag aaattgcctt gtggactttg gtgttcttgc cgattttagg
    30241 aatcgtcatc tggtattacc agaaattcag ttctcgtatt taccgaaata tgcgggaaaa
    30301 attaagtcag ctgaacacga aattgaatga atcgatttcc ggcatgcgta ttatccagca
    30361 gtttcgccaa gaagctcgtt tagctaaaga atttgaacaa gtgaacaacg agtatttaga
    30421 cacgcgctat gcgatgatca aaacgaattc gattctgtta agtccgatca tcaattttct
    30481 ctatgcattg gcgattgcgt tggcgttgac tttgtttggg atcgatgcgt tgcattctcc
    30541 cgtagaagtt ggactgatct atgcatttac gacctatgtc cagggatttt ttaatccaat
    30601 gtctcaaatg atggactttt tgagcacctt tacagatggg atggttgccg gtagtcgtat
    30661 tttgaaaatc atggatacag aagaatatgc accgcaacaa aatcctggag cagatcaagc
    30721 gattacttca ggtaagatcg agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa
    30781 tgtattgaac aatatctctt ttgtcgctaa tccgggtgag accgttgcat tagtggggca
    30841 caccggaagc ggaaaaagct cgatcatcaa cgtattgatg cggttttacg aattttatga
    30901 aggccaaatc ttgattgatg atcatgacat tcgagaatat ccaatggaag agttacgtaa
    30961 gaaaatggga ttggtcttgc aagatgcctt cttgttttat ggagatattg caggaaatat
    31021 ccggatgatg aatccagcta tcacagacga acagattcgc catgcagctg agtttgttca
    31081 agcaaacaag ttcatcgatg aattaccaaa aggatatcac tcaaaagtga tcgaacacgg
    31141 tgccagctat tctagtggac aacgacagct gatcactttt gcgcgaacaa tcgtgacaga
    31201 tcctaaaata ctagtattgg atgaagcaac agcaaatatc gatactgaaa ccgaaggatt
    31261 gatccaagaa ggattggcaa aaatgcgaca aggacggaca acgattgcga ttgcccatcg
    31321 gctgtccacg attcgggatg cagaattgat tttagtttta gacaaaggtc atatagtaga
    31381 gcgtgggaat catgaaacac ttctagcgca aaatggtttg tatgcagata tgtacaaact
    31441 gcaaagcagt ggtgtgtaaa aagaggttgt gacagtagca tagtagcatt ttgtcctaag
    31501 cgctagagtg aaaataaaac agagcttccc agattcttca tctgtgagcg ttagtggcgt
    31561 agtcacccga tttaaaaaag acgacgacag ctagtcaaaa gtgcctaata gccactaaga
    31621 aagcgttgac aaaagcgttt gctttcgata atataaagaa aacgatacaa cttggatagt
    31681 gattgcctga tgcaagctaa acctgatgag tctcccatgg ttccatggac ggctttttgg
    31741 gtttttcttt ttgttttgaa tacagcccta tatatcatgg aaaggagcaa aacatgctga
    31801 ttgagaaagt actgaataac aacgtcgtaa taaccaggga tgaaagaaat caggaaatga
    31861 tcgtgatggg aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt tttgactcat
    31921 gcatcgacaa aaaatttcag ttgacaaatg acgggctttc acaacagttc caagaattgg
    31981 cacaagcgat tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag
    32041 aggctctaca agtccagttg aatgattctg tgtatattgc attgacggat catttgtata
    32101 cggctatcga acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg tatgatatca
    32161 aacaactatt tcctaaagag tatgccatcg ggaaaaaaaa cagtgaagaa aatctcggat
    32221 aaataccaca tcaagttatc agaagatgaa gccggattta tcgcattgca tttagtcaat
    32281 gcacaaggca ttcaaagcca acaaaacaat acctacctgt ttactataac aatacaggat
    32341 attctaaata tcgtccgcta ttactataag attacatttg atgaagattc agtccatttt
    32401 tttcgattcg tcacacactt acgttttttt ctgacacgcg tcctagatgg agaaaaatca
    32461 gaagtgggag aagtttctga ggaattgttt caggttattc aaaaaaaata taaggatacg
    32521 tttggttgcg ttgagaaaat agcagacttt ttagccgaga cgttcaacta ccagatgtcc
    32581 acggacgaaa aaatgtattt aacgatccat actgcgcgtt tagtgcatga gaatcgttgt
    32641 aaataaataa aaaaaggagg ataaattaac aagaacgtat gaaataaggg tagtgacatt
    32701 cagttggcta aaccttccac taatatattg gagggaatag aaatgggaaa ataccaagaa
    32761 ttagcgagaa aaatcgtaga aaatgtcggt gggaaggaaa atatcaatgg tttgacacat
    32821 tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg
    32881 aaaaatatgg acggtattgt aacggtaatg cgagcgggag ggcagtatca agttgtcatc
    32941 gggaatcatg tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat
    33001 gaggctgttt caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc
    33061 tgtttccaac cattccttgc tatcttagct gctggcggga tgattcgtgg actgacagca
    33121 ttccttgtct tcttaggcgc gtttgaccgc ggttctggca catttgttat gtttgataat
    33181 atcggtgatt ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa
    33241 tttaaagtaa atgaattcgt agggatgttg cttggcgcag cattaatgaa tcctagcttg
    33301 tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa cgaccatatt ttctggcact
    33361 atttttgaag cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac
    33421 gcttcttctg tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca
    33481 attaaaaagt tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt
    33541 attacaatgc ctttaggttt tttactcgtt ggaccagtat tcacattcgc aactgatatt
    33601 ttaatggctg gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta
    33661 ggcttcttct ggcaaattct ggtgatgttt ggtctccatt gggcaatcgt accaatggga
    33721 ttgatgcaat tttctgtgaa tggttggcaa aatattatga cacctgttgc agtagtgtct
    33781 tttggacaaa ctgcagcact taccgcactc tactttaagc ttagaaatcc aaaagacaaa
    33841 gcaattgcaa taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat
    33901 gggtttacat taccacgtaa aaaaatattt atttttactt gtgtcggtgg tgcgttaggg
    33961 ggcgcgtata gtgggttaat gaatttaact tcttggaatc aaggtggact aggcatcttt
    34021 acaattccca actatattag accagatggt gatttaacag atgtgattaa tgtattgatt
    34081 gggatcgcca ttgcgatggt ggtatccttt gcgctgactt ttttcttttg gaaggaggaa
    34141 gcaggtgaaa cagatgacag acagaaaaga tcaggtaaag aaattgtaaa aacaccgata
    34201 caaggacaaa tcgctccact gaaagcagca aaggatgcag cttttgcaca aggaacacta
    34261 ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca
    34321 gtcatgaccc tgttccccac gaaacatgca atcggaatgg tttctgaaac aggtctggaa
    34381 ttgctgattc atgtcggatt ggatactgtt cagttggaag ggaaatattt tgaaagcttg
    34441 gtgcagcaag gagaaagagt gaaacaaggc gatctgttgc tccgcttcga caaaaagaaa
    34501 atcgaaaagg aaggatacag tttagaaaca cctgtaatcg tcaccaatta catggattat
    34561 ttggatattt tggaaaatca aggggagacg gtcgggccag acgatgcact tatcaccgca
    34621 ctgacctgaa cggttaggtg aaagaatgga atagacttaa ctattctttt tagtccactt
    34681 ctttattttc aaaattgaaa ggctctttca tgatggctga gctgataggg cgtttatcaa
    34741 atttttgtcc tacagaaatt agatggacta aattctgttg ataattttct gcaaaaacac
    34801 aactatataa cacgtctaga agataacaaa tcgatgtatt gatcgtaaaa ttaccaattt
    34861 ttgaatacag tcgttcgcgt gtcgtaagtc gcaaagcggc tgtactcatt gaggatagcg
    34921 ttgaatcacc gatactggtc aacgctaaaa ttggaacatg attttgtttc aatatttctg
    34981 cggtacgaag gatcacattg ttttcacccg tataagaaat cagaatcccg cacatatcac
    35041 tcgtgcaatt gtacgcttca tagaacgatt caccaaaagt ggtgacaatc gtgacatcca
    35101 ttttgatgcg cttcattttc agcatgaaat cttgagaaat caaagtattc gagttactgg
    35161 cgaaaatcac gatttttttt gcttgaagca ttagttgttt ggctttttgc agttggtcat
    35221 gatgaatgag agaaagtgta tcttgaatcg tcatctgttc taaagaagcc attttatttg
    35281 cgatcgtcat aataccgtct gtggtggaaa aaggaagatt cgcatctacg tcttgaaaat
    35341 tcccttcaag ataggctgtt tcagctaaaa aagcattacg aaaactgacc cagccatcaa
    35401 aacccatttt ttttgctaca cggatgagtg tggacgggtg gacataacat cgctcagcaa
    35461 tctcttggat ggtcatttcg ccaagtgctg gagaatcgct taagagaaac tgaatcactt
    35521 cattttctga aggagaaaag gagatttctt tgattttttc acgtaataac atgtagatac
    35581 cttcttctag tttttttacg taaattataa cttttttgaa aatgatttgt aaacggattt
    35641 atgttatata gcttaataaa gtcatttttt gacaagatat ttacaaaaat ccagtagaaa
    35701 acactaactt ttgtaaatgt tactggaaag aaacgaattt ctattgtttg ttttgtttac
    35761 aaaaaagata ataaaagtgt aaaaagcaaa ggagtgagaa catggcaaaa ggaatcaaaa
    35821 tcgctacgat tggtggaggc agcagttaca cccccgagtt gatggaagga tttatcaaaa
    35881 gatatgagga attaccgata cgagaaatct ggctcgtaga tgtagaagaa ggaaaagaaa
    35941 agcttgagat cgtcagtcag atgtctcagc gtatgtggga tgcttctccg tatgaagtca
    36001 agatctatgc aactttggat cgggaagaag cattgaaaga tgcggatttt gttacgactc
    36061 agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga gcggatccct agttattatg
    36121 ggatgattgg gcaagaaaca aatggtgctg gcgggatatt caaagctttt cgtaccgttc
    36181 cagtggtttt ggcaatcgtt gaagacatga agcgtttatg tccaaatgct tggctgatca
    36241 atttcgcgaa cccaagcggg atggtgacag aagcagttat cagatatggc aaatgggata
    36301 aagtgatcgg attatgcaat gtgccagtaa tggcgacgat gacagaacca gcaatgcttg
    36361 gcaagaaaaa agaagagctg atctacaagt ttgccggtct aaatcacttc cattggcaca
    36421 aagtagcaga taataaagga aatgaccgca cagcagattt gattgatcgt ttatacgatg
    36481 aagacaatgg attgccgaaa aatatctttg acatcccgtt tttcaaagaa caattaaaac
    36541 aaatgaacat gatcccttgt ggctaccacc gctattacta ccgtgaagaa gaaatgttgg
    36601 aacatgcttt agaagaatat caaacgattg gtacaagagc gcaacaagtg aaagaaacag
    36661 aagcagagct gtttgaatta tacaaagatc cagaattgga tcataaacct gaacaattgc
    36721 aaaaacgagg tggtgcttat tattcagatg ctgcctgtga aacgattgct tcgatctatg
    36781 gaaataaaga aacacagatc gtggtgtcta caaagaatga tggagctgtt cctgatctgc
    36841 cggcagattg tgtagtagaa gtcactgcct atgttggcgc acaaggcgca cgaaatgtag
    36901 cgtttggttc ccttcctgct gctgaaagag gctggttgca agtaatgaag aatatggaat
    36961 tgctgacgat cgaagcagcc gtgactggtg attatggaac agctttacaa gcgtttacga
    37021 tcaatccgct tatacgatca ggaaagacag caaaacggat catggatgag ttgtttattg
    37081 cacatcagca gtatttaccg caattcaagg aaacaatcag ccggttaaaa gcagaaggaa
    37141 tcgaagtcca agatgaactg gcaagagaat tgtcaagcaa tacacatgta atggaggaag
    37201 tatgatggga ttcagaaaag attttttatg gggcggtgct acagcagcca atcaatgcga
    37261 gggtggttat gatgaaggcg gacgtgggtt agcgaacgta gatgttgtgc caatcggaga
    37321 agaccgtttt cctgtcatta ctggaaaaaa gaaaatgttt gaatttgata aagatcattt
    37381 ttatccagca aaaattgcga tcgatatgta tcatcattat aaggaagata ttgctttgtt
    37441 tgcagaaatg gggttcaaga cttatcggat gtccattgct tggagtcgta tttttccaat
    37501 gggcgatgaa aatgagccaa atgaatctgg tttgaaattt tatgaggatg tctttaaaga
    37561 atgtcaaaag tacgggatcg agccgcttgt gacgattaca cattttgatt gtccgatgca
    37621 tttgatcaaa gaatatggtg cgtggcgttc tagagaactc gttggttttt atgaaaatct
    37681 gtgccgagtc atcttcaatc gctataaagg acttgtcaaa tattggttga cattcaatga
    37741 gatcaatatg atccttcatg caccattcat gggcgcaggg ctgtattttg aagaaggaga
    37801 aaatgaagaa caaatcaaat atcaagcggc acatcatgag ttggttgcaa gtgccatcgc
    37861 aacgaagatt gctcatgaag tagatccgga gaacaaagta ggctgtatgc tggcagcagg
    37921 agcatattat ccatacactt gcaatcctga agatgtttgg gcttctagaa aatcagatcg
    37981 agaaaattat ttcttcattg atgtccagtc acgaggagaa tatcccgctt atgcattgaa
    38041 agaaatggaa cgaaatggca ttcagatcaa aatggaacct ggggatgccg cattgctgaa
    38101 agaacatact gttgatttta tctccttttc ttattattct tctcgagttt cgactactga
    38161 caacgaactc ttggaacaga ctgcaggaaa tatctttgct tctgtcaaaa atccatatct
    38221 ggaagcaagt gaatggggtt ggcaaattga tccattaggc ttgagaataa cgatgaatga
    38281 tatttatgat cgttatcaaa aaccactatt tattgtagaa aacggcttag gtgcagtcga
    38341 tacgcccgat gagaatggtt atgtggaaga tgactatcga atcgactatc tggcagcaca
    38401 tatccaggca atgaaagata cagtagaatt agatggagtc gatctattag gctatacgac
    38461 ttggggatgt attgatttag tatcagctgg tacaggtgag atgaagaaaa ggtacggatt
    38521 catttatgtc gatcgtgaca acgaaggaaa tggcacatta gcccgttcga agaagaaatc
    38581 attcaactgg tataaaaaag taattgattc aaatggagaa aatctttcga atgattgatc
    38641 aatgatttgt aaaagtctat tgtttttcag cggatttttc gagtataata aagctattcg
    38701 agaaacgcaa gcagaacaat agactttttt tcgcattggc tgtaaagtat gcttacttga
    38761 ttaagtaaag gagaatctac atatgtatga tgtaatcgtt attggcgcag gtcctgctgg
    38821 gatgactgct gctttatatg cttcacgttc aaacttatcc gtattgatga tcgaacaagg
    38881 cgcccctggc ggccaaatga acaatacggc agaaatcgaa aactatcctg gtttcgattc
    38941 aatcatgggt cctgatctag cttataagat gtatgaaggt gtttcaagat ttgggacaga
    39001 aaatgcttat ggaatcgtgc aagatatcaa ggatcacggc agccacaaag aagttatttg
    39061 tgaagataaa acttacgaag gaaaaacagt cattatcgca acaggctgta tccaccgtaa
    39121 attaggcgta cctggcgagg aagaatttgc tggacgtggc gtttcttact gtgcagtttg
    39181 tgatggtgcg tttttccgta acaaacgtct agtagtgatc ggaggaggag actcagcagt
    39241 agaagaagcc atctatttga cacaatttgc ttctgaagta gtcatcgtcc accgtagaga
    39301 tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt gcgaatgaaa aaatttcctt
    39361 tatgtgggat acagtcgttg acgaaatcgt tgggaatgaa atggtagtga caggtgtgaa
    39421 agcacgtaat gtcaaaaccg gcaatgagtt tgatttggca gcagacggag tattcatcta
    39481 tgttggactg gatccattaa ctgcaccatt tagagaagca gggttgacga acgaagctgg
    39541 ctggattcca acagacaacg aaatgaagac gaatatacca ggcgtttttg ccatcggaga
    39601 tgttcgtgaa aaagatttgc gacaaatcac tactgctgtt ggtgatggtg ggatcgctgg
    39661 acaacaagtg tataaatact tggaatcttt agaagaaaca actgcataag tttaaaaaga
    39721 gtggaagaac ggtttcaatt gttcttttac tctttttttt cgcgtgaaat ctactgaatc
    39781 cgatttatgc gaatacttgc aaccgtgctt gataaaagaa tcctttcttg atttaagcgt
    39841 tttttagacc tcgtactttc atatctaata agtgaatgct atacttatta agaaccatat
    39901 ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg tatgaacaat gggtacatga
    39961 agaaaattta gaagaaaact taaaaaaaca attaattgat ctgggtgaag atcctgaaaa
    40021 acttgaagat gctttttatg caccgttgga attcggtact gcaggcatgc ggggaatctt
    40081 aggacctggg atcaatcgaa tgaacatcta tacggtaaga caagcaacag aaggacttgc
    40141 acgtttcatg gatacacaag gtgaagaaac aaaacgccga ggtgtcgcta tcgcttatga
    40201 ctcacgccat caatcaccag aatttgccat ggaagcagcc aaaacattag cacatcacaa
    40261 tatcccttct tatgtattcg aaagtttacg tccaacacca gagctttctt ttgcagtccg
    40321 tcatttgaag acgtttgcgg gaatcatggt aactgcatcc cacaatccag cagcttacaa
    40381 cggatataaa gtctacggag aagatggggg acaaatgcct cctgcagacg cagatgcatt
    40441 gacgaaatat gtccgtgaag tcagcaatcc gttaaaagtt gaagtattat ctgacgacga
    40501 agctgaacat agcggattga tcacgatcat cggagaagaa gtggatgccg cttatctaga
    40561 agaaatcaaa gcagtaacga ttgatcatga attggtggaa acaatgggta aagatttgaa
    40621 acttgtgtac acaccacttc acggaacagg aaaaatgttg ggcgaacgtg cgttgaaaca
    40681 agctggattc gaacaattcg tactagttcc agaacaagct gtcgcagacc cggatttcac
    40741 tacagtcaaa tcaccgaatc cagaagaaca ctctgctttc gagtatgcga tgcgacttgg
    40801 cgagaaagaa ggcgctgact tactgattgc aacagatcct gatgcagacc gtttaggcgc
    40861 agccgttcgt ttgccaaatg gctcctatga agtattgaca gggaaccaaa tcggagccct
    40921 gtttgctcaa tatattttgg aagcacacca atcgcgaggc actttacctg aaaatgcagc
    40981 ggtattgaaa tcaatcgttt ctagcgaatt gcctacagca attgcggaac actatggggc
    41041 taccatgttc aatgtattga caggattcaa attcatcgct gaaaaaattc aacaatttga
    41101 agaagatcat tcccatactt ttatgttcgg attcgaagaa agttacggtt atttagttaa
    41161 acctttcgtt cgcgacaaag acgcgattca agcattgctc ttattagcag aagtagcagc
    41221 ttactataaa aaacaaggca aaacgttgta tgacggcttg caaacgattt ttgaaacata
    41281 tggctactac gcagaaaaaa cgatttctgt gacaatgagc ggacaagaag gttctgcaaa
    41341 aatctctgct ttgatggaaa aattccgtga agaagcaccg acagaatttg ccggagcaaa
    41401 agtcatccaa acagaagact tcaaagagtt gacgagaaca gaccgtgaag gaaacgtaga
    41461 aaaactaacg acaccgccat ctgacgtatt gaagtatacg ctggaagaaa atggctggat
    41521 cgctatccgt ccttcaggaa ctgagccgaa aatcaaattt tatatcggtg taaaaggaaa
    41581 ctcttcagaa gacgcagctg aaaaagtagc cgcttatgaa gcgattatca aaaaaatgac
    41641 agaagaataa aaaaacttga gactgatgga aaaagtggta aactttagaa gtcagtcttt
    41701 tttcaagttg gaggtgaatg gattgccaga cacgcaaaaa acaaaagtag agaaagtcag
    41761 ccaactattc aaaatgttga gtgaccccac tcgtctcaaa attctgctgt atctgaaaga
    41821 cggggaacaa aatgtgacag caatcaccca agcagtagaa atggaacaat ctgctgtatc
    41881 gcatcaactg cgtttattaa gagaaaacca tgtcgtcaag tcccatcgtg agggaaaagc
    41941 gattttgtat agtcttgatg attctcacgt actggatatt ttgaaccaga cgctcaaaca
    42001 tgtggaacat caataacgat aggtgcaaat agagacaaac tggactgtat caacgaatct
    42061 tcttcgctga cacagtccag tttttttaag aaaaagacaa gaaaaactgt tagtctgttt
    42121 cttcggtggt ttcagttcca taataactat gatcttctag gtctaaagca ttcaaaataa
    42181 gtgaagctgt ttcttcgata gataaagaag cgacattgat tactacgcag cccaattttt
    42241 cataaagatc attcgcaaaa tcgagttcag ctcgtatttt ttcgatatcg gaataagcag
    42301 tatcaggatt taatccgtaa gagcgcatcc gttctttacg gatattgttt aaaatatctg
    42361 ggttattcgt cagtcccacg atttttttag gatcgatctc ccaaagctgt ttgggaatat
    42421 gtgcttgtgg gatcaaaggt agattggcaa cctttaaatt tttgttagct aaaaacaagc
    42481 tcaaaggcgt tttggaagtt ctggatacgc ctagcaaaac gacatctgct tctagaaacc
    42541 cacgaggatc tttcccgtca tcgtatttta cagcaaattc cattgctttg atccgtttga
    42601 aatagttctc attcaataaa tgaagggcgc caggttgccg aatgggcttg ataccagtta
    42661 agcgctcgat ttctgatatc ggcggagtca tcacatcaaa actaaataat tgatgttctt
    42721 cacaaaaaga acgcgtcatc tcgatcaatt cttgattgat caatgtgtgc aggacaaggg
    42781 ctttttcttt tagtgcatct tgcagtgcct gcatcaatgt tttctcatct gtcacaaatg
    42841 ttctgcggaa taaattgaac tcaacggtag gatattgggc catagtggct tgcgctaatt
    42901 ttgtcgcagt ttctccagca gagtcagaga tgacaaaaaa agtaacgatt tctgtagaat
    42961 cggaagacat ggataaaacc tcacttgcat tttttttatg tttattatac catactctag
    43021 aatgtgtact tcgtaatcat tttgatttgg aggaataagt catgaaaaaa cagacattga
    43081 ttgaacaatc actagaaaca ttgaaaaata acggttttaa atatactaaa aaaagagaag
    43141 cattgttaac ttatttagtg aagcgcaatc gctacgtatc cgctaaagaa gtttttgact
    43201 atatgaatca ggaattcaaa ggagtaagtt acgatacgat ttatcgaaat ctccatgatt
    43261 ttgcagaaat cgatttactg gaagaaaccg atttaaatgg agagatgaag tttcgttttc
    43321 attgctgtca aggggaaatc ggccatcacc atcactttat ctgtacggtg tgcggaacaa
    43381 cgaaagaaat ccatatgtgt ccaatgaatt atttcgaaga acagctcggt ggatgtacga
    43441 tcgaagggca tagattcgag attttcggaa gatgtgaaaa ttgtcaataa ggatttaaat
    43501 aaaataacat ttgataattt taaaaaaaag caagatttcc ttgacggtca aaacagcatt
    43561 agatataata tttacaggac agtcttgttt tttgagatat atttcaataa caattacggt
    43621 tactttttaa gctcggaggg agggaattaa catgtcaaaa acagtcgttc gcaaaaacga
    43681 atcacttgac gatgctcttc gtcgcttcaa acgttccgtg tcaaaagcgg gtactttaca
    43741 agaatctcgt aaacgtgaat tctacgaaaa accaagtgta agacgcaaga aaaaatctga
    43801 agcagctaga aaacgcaaaa aattctagtt ctgtgatgaa ttggagttgg tagtgtgtca
    43861 ctactaagta gattaaacga tgacatgaaa acagcgatga aagcgaaaga taaggaatct
    43921 ttacaagtca tccgaatgat caagtcatct atacaaaacg agcagattaa agaaggtcac
    43981 gacttgacag aagaagaaga attaacggtg ttgtctcgcg aaatgaaaca gcgacgtgat
    44041 tctttgcatg agtttgaaga agcaggacgt gacgatttag ctgaaaaagt aaaaagtgaa
    44101 attgtgatag tcgaaaaata catgccggaa caactttccg acgaagaaat tcgacaactt
    44161 gttcaagaag caatcacaca aactggtgct tcatcaatga aagaatttgg caaagtcatg
    44221 ggtgcgatca tgcctaaagt caaaggaaaa gcagatggaa accaagtaaa tgccattgtg
    44281 aaagaactat tacaatctta attttatcat ttaaactgcg gacaatcatt gtccgcagtt
    44341 tttttgtgcg cgtaaaaaag agaacaaaaa aatagctaga cgaaagaata ggattctttc
    44401 atctagctgg ttttttcctg tttaatattt cttatgttaa attgtgtttg atttggttat
    44461 tcttctggta gttcatttgc ttcttgttta taccctgata ggcgataggc gtaataaaac
    44521 agtaggctgc cagcgtaacc aagtaacgtt gggaccatcc agccaagtcc taatgcagca
    44581 aaaggaacgt gatgatgata aaattcgagc atgcttgcta caactctttg ttgactcact
    44641 aaagcaggcg aatttgccag catatccaat acggcaggaa cagcagcaaa caacatcgtt
    44701 acttgataga caaatggtgt cttttggaaa aattttgctg ttaaggaaag caagatcaac
    44761 gcaagcgaca atggatacaa aagcatcaat actggtaaag accattggat gatattgtct
    44821 aaaccagcat ttgcaacgac aaaagagacg aaagtcgtta accgcagcca tgtcatataa
    44881 ctgactttag ggaacagttt atggaaatct tgcgcaaaag aaacaacgag tcccatagcg
    44941 gtagtgaaga cacctaatgt gaccatcact cctaagaagc tagcgccgaa gttacctaaa
    45001 taaaaattag tgatctgaga aagtgcagtt ccgccatttt ctgataaaga catcttattc
    45061 aaactcatcg caccgagaag aacaagacca aaatagatca tgacttctag cgcaacgctc
    45121 aggctaccag cttttgctgt taattctgtg acttttttat ctttgaaacc aaggtcacgg
    45181 actgcatgga caaaagtgac acttaaggca agcaaggcta ctgcatcaac cgtgttataa
    45241 ccttctagga caccatttgt taaagcgttt gtttggtaag tatctgcagc agattgattt
    45301 agatttccca ttgggtggaa taaagaaacg acaaaaatga ttgctaacaa tgcaagaaac
    45361 aaggagttta gatactttcc gacgtatttg accaaccgtg tgggatggac agtaaggaaa
    45421 taagcaagta aaaagaaaag agcagagaat aaaaacatac ctaatgaatt atatttttca
    45481 ggcaaaaatg gtttagcagc catttcaaaa gcagtagcag ctgtccgtgg tgtaccgaaa
    45541 aatggtccga tcgtcaaatg aacaaggatt aagaacaagg cagcatacca ttttccgaca
    45601 ggtttagcta agtcatacag accatcactt tttgtcacta ctacagctaa gatagctagt
    45661 aatgggaaaa gagcgcccga gatggcaaat cctacgccag cactgaccca gtttgcgcca
    45721 gccatttgac ctaagtggac aggaaagatt agattacctg acccgaagaa catcccgaag
    45781 ataagtgaac tgaccatcaa tagttgtttc catgaataag tttttgtttc ttccataaat
    45841 ttttcctcca ataattatca ttttgattga cgatataaac gttgcagcaa ttagtaatgc
    45901 cactaatatc agattccatg aaaaccacca ccttaaaata taaaaaaagc ccttttccac
    45961 ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa aaaagcttct
    46021 tcattcacgg acgctttttg ataaagttat ccgctagcct gataatggag gcagccacaa
    46081 tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt tattgtcatt
    46141 tctcacttac atgactcact gggaataaat cgttgaatag cctactgttt tcctcgacgc
    46201 tttgctgtat gtttatttgt tgacgtaata atacacctgt tttttttgaa atgcaagaat
    46261 attttaacta ttttgttttc tttttttgaa acaagaaaaa tacctagaga atacctgctg
    46321 aatgaaacgg aatattttgt tctccacttt catataactt acattctcag cttcttttta
    46381 tcaaaacgag acctttagcg aatggaaacg gctatttttt aacgtttctt ggtacgtatg
    46441 gttaaacgac tcttttacaa ccttttctta tttgctatat agtttcttct gagtatggta
    46501 aaataggaaa gaatgaatcg aaaaaaaggg gatgtctatt tttgacggaa gagtctagtt
    46561 ctttagagat tagattgaca gctgctgatg atgtcagcat gcttttaggt gcacacgaca
    46621 agcatataaa actgatcgag gaaacaacgg aaacaaccat ccatacacgt ggagaaatca
    46681 ttcagatcat tggtgagcaa tcccagattt ccttggctgc ctcagtcatt cggacattac
    46741 aggaattgat caaacgagga atccacgtaa gtacgccaga tgttgtgacg gcattgaaga
    46801 tggggcaaaa aggtacatta gattattttg ttgagatgta tgaggaagag atcgtcaaag
    46861 accgtaatgg aaaacctata cgggtaaaaa acagcggtca aaagaaatac gttgaatcgg
    46921 tagccaaaca tgacatcgta tttggtgtag ggccagccgg aacagggaaa acgtttcttg
    46981 ccgtagtatt agcaattgct gccctaaaaa aaggcgaagt gcaaaaaatc attttgacgc
    47041 ggcccgctgt ggaggcaggg gaaaacctag gctttctacc cggggatctg aaagaaaaag
    47101 tggatcctta tctgcggcct gtttatgatg ctttatacca aatttttgga ttggatcata
    47161 cgaaccgatt gatggaacga ggcgtgatcg aaatcgcacc tttagcttac atgcgggggc
    47221 ggacattgga tgatgcattc gttatcttgg acgaagcaca aaacacgaca gtcgctcaga
    47281 tgaaaatgtt cttgacccgt ctcggttatc aatcaaaaat gattgtcaat ggggatacaa
    47341 gtcagataga cttgcctcgt gggacgacaa gtggtcttgt tcatgcagaa cgaacattaa
    47401 agcaaatcaa aaaaatcgat tttgttaatt ttgaagcaag tgacgttgtt cggcaccctg
    47461 tagttgccga aatcatcaaa gcttatgaaa aggcggatct tcatcaagaa taagctttgc
    47521 ttaggaggat aagcacatgt tgaataaaac actgcagaaa ctacaaaaaa aactcggaaa
    47581 ggcagcattg cctttccttc tgttcttgtt ttttatcatt gtctgcggcg ttacatttag
    47641 tagtgtcaaa caaaaggcca atgatttcag agaaggccaa gtagcagaag agagtatccg
    47701 tgcgaataaa acgattgaga acacggaaga aaccgaacaa agaagaaaac tggcagcaga
    47761 agcagtgact ccagaatata cttatcaaaa agatctggca gatgaccaga acaatcggat
    47821 caagcaatta ttcgaattga tcgataagac gaatgatacc atcaacaaat cttataatga
    47881 caaggtagat aaggcgaagg aagatgaaaa tatcccgaaa cctactacag aagagcggat
    47941 tgctacactg aagaaaaatt ttgaaaacgt gaatgcagaa aacgttgcgt tttaccaaaa
    48001 actgccgaac aatttttatt cgacgatatt tgaaatgact gaaacggaaa ttcacacggt
    48061 tagggatgaa agtttgaagc tcattgacga acagatgagt aaacaagttc gtgaaagcga
    48121 attggaagct tttaaacaag aagcagaaga tcagattcaa tatttgaatg tcacaacaga
    48181 gcagcaacaa atgattcgct acttagtgga tcaaggcatt gtagttaatg atgtattgaa
    48241 tgagaaaaag acagaagaat taaaacaatc tgcacgagaa gctgtacaac cagtgatgat
    48301 cttccaagga gaaattattg ttcgcgaagg aaatcaaatt gatgcttcag cgatgaaaaa
    48361 attggaatta ctaggattga cgaatcaaac gacttctatt ttcccactca ttgccatggt
    48421 tttagctgtt ttgcttcaaa tcggtgtact gatttataat tcacttcaat tccaaaatgc
    48481 aggagaaaga acaaaatacg tacttttcta cgtgacggca atgtcagtaa gcgtcgtact
    48541 aatgaaattt ttccaactct tccaaacaga acaagctgct tatattccgc tgttttaccc
    48601 cgcggctttt gctccacttg ttttaagctt tttcttgaat cgaagagcag ggatcatggc
    48661 tggtattttt caagcagtat ccgcactttt cattttttat gattcgattg gcacgaactt
    48721 tctgacagtt attttgatgt cctatgtatt ttctggtctc atggcaacga tcgtcagacg
    48781 caaacggatc tcagaacaag ggttatccgc tgctatgtgg gtcattattt ttcctgtgat
    48841 gatggatgtc attttgatta tctaccaagg aatgagcttt ggggatgcaa cgacttggtt
    48901 aacgctagta tgcggattca caggtgcact gttctcattc ttgctgacga taggtctgca
    48961 cccctatatt gaattgatgg tcaatgatga tagtgtgatc gtattaaatg aactcagcaa
    49021 tcctaatcat cctttattga aacagttgct ggaagaagca ccaggcacgt atcaccatag
    49081 catgatggta gccaatctaa gtgctaatgc tgtagcagaa atcggtggac gttctttact
    49141 aactcgagta gcttgctatt atcatgatat cggcaagatc aaacacgcca gctttttcgt
    49201 tgagaacttg ccttcaggag cggaaaatcc gcacaatttc ttattgccag aagacagtaa
    49261 acatattatc tttggacatg tgacagatgg tgccaaaata ttggaagatt ataatatgcc
    49321 gcaaatggtg atcgatattt gcagacagca tcatggaacc acgctgatgc gttatttcta
    49381 cgttaaggca aaggaacgca acccggaagt cacggaagag caattccgtt atcctggacc
    49441 aaaacctcaa agccgtgaag caggaatcgt caatatagct gacagttgtg aagcggcagt
    49501 acgagcaatg gaccatccaa cgagcgaaaa aatcgaaagt tttgtccata atcttattga
    49561 agagcgtatt tccgatggac aattggacga cagtggattg acgttaaaag aaatccgtaa
    49621 agtagaaaaa tcattgatca gcggtttaag cagtaccttc cattctcgga tcaaatatcc
    49681 taaaatgaaa tcggaagttg agaagataaa agaagaacaa gaagaataga aagaagcgat
    49741 gtataatgga tattactttc atggatgaaa caaaccaagt ctcagaagag aaaatgaaag
    49801 aaatcgacga cttgctgcaa tttgcagcat catatttaga gttgccagaa gaaacagaaa
    49861 tgtctgttac tttcatggac aatgcagcga tccaagtaat caatcgtgat taccgtggaa
    49921 aagatgcacc gacagacgtc atcagttttg ccttggaaga agagggcgaa gacgaaatcc
    49981 cagtgatttt tgaagatgga gagaatccat tgcctcgtgt actgggagac atcatgatct
    50041 ctactgaacg tgctgcagag caagcagaag cttacggcca ttcgtatgat cgggaacttg
    50101 gctttttagc ggttcatgga ttcttgcata tcaacgggta tgaccatatg acaccagagg
    50161 atgagaaaga aatgtttggt ttacagaaag agatcttaga tgcctatgga cttaaaagat
    50221 aaaaacgtag agaaaaacaa acatttcctg atgtccgtgg aatttgccgt ccaaggagtc
    50281 aaaaccgttt ttgaggaaga acgcaacatg aggaaacatg ttgcgttcgg aatcctcgct
    50341 ttgtttatgg gcgtgatttt ccagcttgat cggatagaat ggttatggct gcttttagct
    50401 gtttttctcg tatggatagt agagatcatc aatactgttt ttgaaaatgt cgtggacatg
    50461 tttacggatt ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca
    50521 gttttagtga ccgcttgttt ttccgtcatc gttgggttga ttttatttgt ccctaaaata
    50581 tatcatttgt tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg
    50641 tcgcaatcgt cggccgcccg aatgtaggaa aatccacatt attgaatcga atcgtgggtc
    50701 aaaaaattgc catcatgagt gataaggcgc agacaactag aaataaaatc caaggagtat
    50761 atactacacc agaagctcag ttgattttta ttgacacacc aggaatccat aaaccaaagc
    50821 atcgtttagg agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggacgcgg
    50881 tcttatttat gatcagcgct gatcaaaaac gcggtcgggg agacgatttt attattgaac
    50941 gtctaaaaaa cgtgcattct cctgtttatc tgattatcaa taaaatcgat aaaatccatc
    51001 cagacgagtt gttgggaatc atcgaagatt attcaacgca gatgccgttt gcacaagtga
    51061 ttccaatttc tgctacagaa ggaaacaacg tcgaacgttt gatggatgta ttagtcagtg
    51121 aaatgccaga gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact
    51181 tcatcgtttc tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc
    51241 attctgtcgc agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc
    51301 aggctacgat tattgtcgaa agagatagcc aaaaagggat cattatcggc aaaggcggaa
    51361 aaatgctgaa gcaagtagga acgaaagcga gaaaagatat cgaaaatctt ttaggagaca
    51421 aagtctttct agaactttgg gtcaaagttc aaaaagactg gcgagacaaa aaagtctatc
    51481 tgcaagactt cggctaccga aaagacgaat attgatattc tgagcttctt gtaagttagg
    51541 tatagaaata aactggataa aaatccgcgt gttgagaaaa gctgcggaag atcgcaacat
    51601 aaatgatctg gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg
    51661 agccagcttg cagaatcaaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat
    51721 aaattagtca aaatctttac ggaatcttct ggcaaaatga tgttttatat caaagggatc
    51781 catcggcaaa atcagccctt agctccggct ttgctccctt ttactgaagc agtttatatc
    51841 gggaattttc gaagtgaagg tttgtcattt ttaagcagtg tcaaagatgt ccacgcgttt
    51901 cgacacatcc aagaagacat ctttttgagc ggctatggca catatttatt gaatctagta
    51961 gatgcggcaa tcgaagatca tcagtacgac ccgcatttgt tccagtttac gcaacaagca
    52021 ctgcaactga tggaccaagg agatgatgca gaaatcatca cgaatatttt tgaagtccaa
    52081 atactgcaac gcttcggaat tgcaccaatc tggacacact gcgtcgtatg tggggaaaca
    52141 aaaggaaaat ttgactattc ttccaaatat ggtggtgtga tatgcgaaag acactggtcg
    52201 atggatgatc accgttatca tgcagatcct cgagctgttt atttcgttcg gatgttttca
    52261 gctatttctt atgataaaat cagtggaatc catctgaaag aagaaaccaa acaagcaatc
    52321 cgtgaactca tcgaccagct gtatgatgaa tatgtcggta tccatttgaa aagcaaaaag
    52381 ttcattgatc aaatgaagtc ttgggcacac gtattaaaga cgtcagagaa agacatgtct
    52441 gaaaaagatc tacctaaaaa agaagaatag taaatgaaac atacaacaaa aaatcaaaac
    52501 catttgacaa acacagggag ttccgtctat tattaaccgt agatcaaata gatcgttcac
    52561 tgagaggatc tattatagaa acaagaaaac tgcaaaatga atacgtacat tgaccaagat
    52621 cagtaaaaaa cacgtggttt agcgagttcg ggagagtggg agccggaccc aaacgatttt
    52681 tgaagaggca cttgcgagta cgataaatga agctgccgaa tctttattcg gaagtagggt
    52741 ggaaccgcga attattcgtc cctatgtctg taatcagaca tagggatttt ttgtcgtttt
    52801 ctttcctgta ttcttatttg acagcaacgc tttaaaattg aaaggggcaa aacagatgag
    52861 taaaaaattg accgtccaag atatgatttt aacattacaa aaattttggt catcaaacgg
    52921 ctgcatgctg atgcaagcgt atgacacaga aaaaggagca ggtacgatga gcccatatac
    52981 ctttttacgg gcaatcggac cagaaccatg gaatgctgct tacgtagagc catctcgccg
    53041 accagctgat ggacgttatg gcgaaaaccc aaaccgtttg tatcagcatc atcaattcca
    53101 agtcgtgatg aaaccttcac ctgagaatat ccaagagctt tatttagaaa gcttgcgcct
    53161 tttaggaatc gatccactag aacacgacat ccgttttgtt gaagacaact gggagaaccc
    53221 atccatgggc tgtgccggtg tgggttggga agtatggctt gacggaatgg aaatcacaca
    53281 attcacgtac ttccaacaag ttggtggact gccttgtaaa ccagtaactt ctgagatcac
    53341 ttatggatta gaacgtcttg cttcttatat ccaagaagtt gaaagtgtct atgatttaga
    53401 atgggctgat ggcgtgaaat atggcgagat cttcaaacaa ccagaatatg aacattctaa
    53461 atattctttt gaagtaagcg accaagactt gctactaagc aactttgatc gttttgaaaa
    53521 agaagctaaa cgctgtatcg atgaaaatct tgttcatccg gcgtatgatt atatcttgaa
    53581 atgtagccat acattcaact tattagacgc ccgcggtgca gtatccgtaa cagagcgtgc
    53641 cggttatctt tctcgcatcc gcaacatggc tcgtgctgta gccaaaatct ttgtggcaga
    53701 acgcgaaaaa ttaggattcc ctttgttgaa taaaaacaca gcaaatccag aaaacgtgaa
    53761 ggaggctgaa taagatggca aaagacttat tacttgaaat cgggctggaa gaaatgcctg
    53821 ctcatgttgt cacaccaagc atgaaacaat tagaagaaaa aacagcaaaa tttttagatg
    53881 aaaaccaatt gacttatgat acgatcgaaa cattttccac accgcgccgt ttagcaatca
    53941 aagtaacaaa cattccagaa cgacaagcgg acagtgaaga agaagtcaaa ggaccagcca
    54001 aaaaaattgc tttggacgca gatggaaact ggacgaaagc agcggaagga tttgtccgcg
    54061 gacaaggtct gacgacagac gctatcactt ttagagaatt aaaaggtgtg gaatatgtct
    54121 atgtaacgaa atacactcat ggtaaggaat ccaaagaagt attgacaggt ttagcagatg
    54181 tcattaaaag cctgactttc cctgtaacga tgcattgggc agaatacgat tttgaatata
    54241 tccgccctat tcactggatc gtggcattgt tagatgacga agtaatccca ttcgaaatct
    54301 tggatgtcaa aacaggcaga gcctctcgag gtcatcgttt cttaggcgaa gatgtgacat
    54361 ttgcgcatgc aaaagaatat gaagaaaaat tgaatgaaca attcgtcatt gcgtctccgc
    54421 aaaaaagaca agaaatgatc gttacacaag ccaatgaatt agccgaaaaa aatcattggg
    54481 ttattgatct agacgaagat ttactagaag aagtcaacaa tttagtcgaa tatccaactg
    54541 tcttttctgg cgacttcgaa gaaaaatatc tttctgttcc tgaagaagta ctagtcactt
    54601 ctatgaaaga acatcaacgt tatttcgagg tacggaatga tcaaggtatg ctgcttcctc
    54661 attttatttc tgctcgtaat ggtgatcggg taaaattgga aaatgtggtt cgaggcaatc
    54721 aaaaagtatt gacggctcgt ttagaagatg cggaattctt ttataatgaa gacaagaaag
    54781 cttcaatcga ccaatacgtt gaaaaactaa aaactgttac tttccatgaa aaaatcggta
    54841 gtatctatga gaaaatgcaa cgggtacaag tcattgcccg tatcattggc gaacaagtag
    54901 gactatctga ggaagaattg atggatctgc aacgagctgc tgaaatctat aaatttgatc
    54961 tagtaaccaa catggtaggg gaatttcctg aactgcaagg gatcatgggc gaaaaatatg
    55021 ctctgctgaa aggcgagaaa cctgctgtag cgacagcgat tcgtgagcat tacatgccta
    55081 tatccagtga aggagagcta cctcagtcta cagtaggttc tgttttagcg attgcagata
    55141 aattagatag catcttttct ttctttgctg ttgggatgat cccaagcggt tcaaacgatc
    55201 cttatgcctt acgtcgccaa gcatacggtg tcgttcggat catcgaatcg aaaaaatggg
    55261 cctttccttt gtctgttctg caagaaacga ttagcgatgt tatcagtaaa gatacggatc
    55321 gtttcggcat tggtttatca gaagggcaac aacaagtgat cgactttatc aaaggccgtc
    55381 tgcgccaatt attgacaacg aagaatattc gtcatgatgt catcgatgct gttttgaatg
    55441 cggaacaaaa agatctgaca aaagtatttg cagcagcaca attgttcaaa caacatcttg
    55501 cagatgaaga cttcaaacct tcaatggaag ctttgacacg tgtcgtgaac ttagcgaaaa
    55561 aagcgcagct tgaaaaacaa tcagaagtag atcctgagct gttcgaaaac gacgcagaga
    55621 aagaattaca caaagcagta gaagcaatga aagcaagttt tgctgaccaa acgatccacg
    55681 aaaactacga ccagcttgtc gctcttcgtc cactgatcga aaattatttt gatcaaacga
    55741 tggtcatggc tgatgaacca gcagtacgta agaaccgact aacacaactt agtcaaatag
    55801 ctgccatggc tttgtcatta gcaagcttgg atcaaatcat cacaaaatag tcttttgttt
    55861 gaaaaacaga gagcatgcac gaaacgtgct gtgtctctgt ttttttgtta taaatataaa
    55921 tagctgttat taattctatt ttaataattt ttgttatttt attctttgtt cgaattttca
    55981 aaatgctcat gaaaagagag gaaataaaaa tgaaaac
//
LOCUS       DACDKL010000002        51716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDKL010000002 DACDKL010000000
VERSION     DACDKL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.002"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            90..668
                     /locus_tag="IV329_000052"
     CDS             90..668
                     /locus_tag="IV329_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939264.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(908..991)
                     /locus_tag="IV329_000053"
     tRNA            complement(908..991)
                     /locus_tag="IV329_000053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Leu,seq:gag)
     gene            1374..2591
                     /locus_tag="IV329_000054"
     CDS             1374..2591
                     /locus_tag="IV329_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8939265.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2775..3611
                     /locus_tag="IV329_000055"
     CDS             2775..3611
                     /locus_tag="IV329_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8939266.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3687..4835
                     /gene="nagA"
                     /locus_tag="IV329_000056"
     CDS             3687..4835
                     /gene="nagA"
                     /locus_tag="IV329_000056"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8939267.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5055..6113
                     /locus_tag="IV329_000057"
     CDS             5055..6113
                     /locus_tag="IV329_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8939268.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6132..7151
                     /locus_tag="IV329_000058"
     CDS             6132..7151
                     /locus_tag="IV329_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8939269.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7253..7969)
                     /locus_tag="IV329_000059"
     CDS             complement(7253..7969)
                     /locus_tag="IV329_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8939270.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7938..8723)
                     /locus_tag="IV329_000060"
     CDS             complement(7938..8723)
                     /locus_tag="IV329_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8939271.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8714..9463)
                     /locus_tag="IV329_000061"
     CDS             complement(8714..9463)
                     /locus_tag="IV329_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8939272.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9761..10285
                     /locus_tag="IV329_000062"
     CDS             9761..10285
                     /locus_tag="IV329_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939273.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10301..11800
                     /locus_tag="IV329_000063"
     CDS             10301..11800
                     /locus_tag="IV329_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8939274.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11888..12490)
                     /locus_tag="IV329_000064"
     CDS             complement(11888..12490)
                     /locus_tag="IV329_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8939275.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12594..13550
                     /locus_tag="IV329_000065"
     CDS             12594..13550
                     /locus_tag="IV329_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8939276.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13855..14811)
                     /locus_tag="IV329_000066"
     CDS             complement(13855..14811)
                     /locus_tag="IV329_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8939277.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14985..15545
                     /locus_tag="IV329_000067"
     CDS             14985..15545
                     /locus_tag="IV329_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939278.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15538..16362
                     /locus_tag="IV329_000068"
     CDS             15538..16362
                     /locus_tag="IV329_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8939279.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16372..16989
                     /locus_tag="IV329_000069"
     CDS             16372..16989
                     /locus_tag="IV329_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8939280.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17007..17690
                     /locus_tag="IV329_000070"
     CDS             17007..17690
                     /locus_tag="IV329_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8939281.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18373..19608
                     /locus_tag="IV329_000071"
                     /pseudo
     CDS             18373..19608
                     /locus_tag="IV329_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19616..20503
                     /locus_tag="IV329_000072"
     CDS             19616..20503
                     /locus_tag="IV329_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939282.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20520..21356
                     /locus_tag="IV329_000073"
     CDS             20520..21356
                     /locus_tag="IV329_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8939283.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21359..21982
                     /locus_tag="IV329_000074"
     CDS             21359..21982
                     /locus_tag="IV329_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939284.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21979..23271
                     /locus_tag="IV329_000075"
     CDS             21979..23271
                     /locus_tag="IV329_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8939285.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23630..23727
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23843..24424
                     /locus_tag="IV329_000076"
     CDS             23843..24424
                     /locus_tag="IV329_000076"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8939286.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24427..25734
                     /locus_tag="IV329_000077"
     CDS             24427..25734
                     /locus_tag="IV329_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8939287.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26027..26515
                     /gene="purE"
                     /locus_tag="IV329_000078"
     CDS             26027..26515
                     /gene="purE"
                     /locus_tag="IV329_000078"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8939288.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26499..27632
                     /gene="purK"
                     /locus_tag="IV329_000079"
     CDS             26499..27632
                     /gene="purK"
                     /locus_tag="IV329_000079"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8939289.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27645..28940
                     /gene="purB"
                     /locus_tag="IV329_000080"
     CDS             27645..28940
                     /gene="purB"
                     /locus_tag="IV329_000080"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8939290.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29120..29497)
                     /locus_tag="IV329_000081"
     CDS             complement(29120..29497)
                     /locus_tag="IV329_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939291.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29753..30481
                     /locus_tag="IV329_000082"
     CDS             29753..30481
                     /locus_tag="IV329_000082"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8939292.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30481..30735
                     /gene="purS"
                     /locus_tag="IV329_000083"
     CDS             30481..30735
                     /gene="purS"
                     /locus_tag="IV329_000083"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8939293.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30737..31408
                     /gene="purQ"
                     /locus_tag="IV329_000084"
     CDS             30737..31408
                     /gene="purQ"
                     /locus_tag="IV329_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8939294.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31409..33631
                     /gene="purL"
                     /locus_tag="IV329_000085"
     CDS             31409..33631
                     /gene="purL"
                     /locus_tag="IV329_000085"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8939295.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33616..35055
                     /locus_tag="IV329_000086"
     CDS             33616..35055
                     /locus_tag="IV329_000086"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8939296.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35087..36130
                     /locus_tag="IV329_000087"
     CDS             35087..36130
                     /locus_tag="IV329_000087"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8939297.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36127..36705
                     /gene="purN"
                     /locus_tag="IV329_000088"
     CDS             36127..36705
                     /gene="purN"
                     /locus_tag="IV329_000088"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8939298.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36702..38237
                     /gene="purH"
                     /locus_tag="IV329_000089"
     CDS             36702..38237
                     /gene="purH"
                     /locus_tag="IV329_000089"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8939299.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38253..39527
                     /gene="purD"
                     /locus_tag="IV329_000090"
     CDS             38253..39527
                     /gene="purD"
                     /locus_tag="IV329_000090"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8939300.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39649..40563
                     /locus_tag="IV329_000091"
     CDS             39649..40563
                     /locus_tag="IV329_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8939301.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40696..42261)
                     /locus_tag="IV329_000092"
     CDS             complement(40696..42261)
                     /locus_tag="IV329_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8939302.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42254..43705)
                     /locus_tag="IV329_000093"
     CDS             complement(42254..43705)
                     /locus_tag="IV329_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8939303.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43719..44735)
                     /locus_tag="IV329_000094"
     CDS             complement(43719..44735)
                     /locus_tag="IV329_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8939304.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            44955..46043
                     /locus_tag="IV329_000095"
     CDS             44955..46043
                     /locus_tag="IV329_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8939305.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46097..47623
                     /locus_tag="IV329_000096"
     CDS             46097..47623
                     /locus_tag="IV329_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8939306.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47625..48830
                     /locus_tag="IV329_000097"
     CDS             47625..48830
                     /locus_tag="IV329_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939307.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49087..50064
                     /gene="guaC"
                     /locus_tag="IV329_000098"
     CDS             49087..50064
                     /gene="guaC"
                     /locus_tag="IV329_000098"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8939308.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50328..>51716
                     /locus_tag="IV329_000099"
     CDS             50328..>51716
                     /locus_tag="IV329_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8939309.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYS"
BASE COUNT        16484 a         8820 c        11339 g        15073 t
ORIGIN      
        1 ataaatggtt tcctgaatat aaaaatttca actttcttaa taagttagat ttaggtattt
       61 catcatttgg aaagccgttt tttttagaca tgaatatctt atttttattg ctatttttct
      121 taattttcct tctaacatat aggaaatgtc aaaactatta tgtgcgtata ttgactgcat
      181 taccgttttt cttaaacctg attatttatt ttggtaatac aatgggtcaa agctttactt
      241 acgtcaatgg aaataaaagg gcaatgattt ggagtagtag taacctgaat aatctcttta
      301 cagagttagg aacgaaactg tcactttttt atccaggtac ttggatagct actttagtag
      361 tcttggcatt attactatgt ttaattgttg gaatttattt aagttttgac aataagaaaa
      421 cttctatttt tttagttatt ttgatgataa tgggattttg ttcaaggtta attatgggat
      481 tttctcccac agtatgggct tccggaatgc ggacgtacta tatactttat gttgttatcg
      541 ccatcttagt actaatggca gttaaagagc taatgaaaag catgagtgta caaaaaaatg
      601 aatttatgca atttggatta accgtgcttg gtatttgtac ctttataata acggtgataa
      661 acagataata tacaaaaaat ccctcgcttt agtattaggt gagggatttt actcgttttt
      721 cgattaattg ttttaacact tgcaagtttc tacgagtagc ttgtttccga ccttcatata
      781 ctcacctcag gctgtttaca tgattgtacg ttctttttgt aaaggataca gtgatatgta
      841 tataatggca taaagaaacg ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga
      901 gttgtagtgc agatgatggg actcgaaccc acacgagcta acgctcacat gaccctcaat
      961 catgcttgtc tgccaattcc aacacatctg cgtaataata acacactaat gatagcgagg
     1021 ttttacatag ttgtcaagct caaaaatggg aggacttatt aagatggaga aatatgacgg
     1081 agaattttca ggactgggaa tgatcttagg tgtattgatt ggacttgcat tcggacgttt
     1141 tttgcttggt tttatgctag gaatcatttg tggaattgca atggattggg cagcgaatct
     1201 atggaatgac tatcatgaca attaagttct caacgcttgt ttgtgttttt ttaatactta
     1261 cgtttttcct aaataagtgg caaaattttc taggatggag tatgatattg aaatagtaaa
     1321 taacaaaaaa tttggaaagt tctaactttt tgaaatgagg gattggctaa gtgatgagag
     1381 aatttgaaaa atccaacaaa ttagaaggtg tcagttacga tgttcgcggt cccgttctag
     1441 aagaagcgga gcggatgcaa gaagagggca ttagtatttt aaaattgaac acgggaaatc
     1501 ccgcaccttt tggatttgaa gcaccaaacg aagtcattcg tgatttgatc atgaatgccc
     1561 gtgactcaga aggctactca gattccaagg ggatcttttc tgctagaaaa gcaattgagc
     1621 aatattgcca actgaaacat tttccgaatg tgacgatcaa tgatatctac accggcaacg
     1681 gagtgagcga attgatcacg atgtgtatgc aggggttgct ggacaatgga gatgaagtac
     1741 tggttccaat gccggactat ccgttgtgga cggcttctat tgcattagct ggaggcacac
     1801 ctgttcatta tatttgcgat gaagaggcag aatggtatcc tgatattgat gatatcaaat
     1861 caaaaataac gagtcggaca aaagctattg ttatcattaa tcctaataat cctactgggg
     1921 cactttatcc aaaagaatta ttagaagaaa tcgttgaagt agctcgacaa aataacctga
     1981 ttatttattc cgacgaaatc tatgatcgct tagtgatgga tggtctagaa catattccga
     2041 ttgctacatt agcgcctgat ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc
     2101 gtgtagcagg atttcgagta ggctggatgg tcctaagtgg agataagtca cacgtcaaag
     2161 gctatataga agggctgaat atgttgtcct ccatgcgttt gtgttccaat gtattgtctc
     2221 agcaaattat ccaaaccgcg ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag
     2281 gcggaaggat ctatgaacaa agagaatata tctataatgc aatcaatgat attcctggtc
     2341 tttcagccgt aaaaccaaaa gctgcttttt atatattccc aaaaattgat acaaagcgat
     2401 tcaatatttt ggatgatgaa aaattcgttt tggacttttt acatgaacat catattctac
     2461 ttgtacatgg tggtggattt aattggaacc agccagatca ttttcgaatc gtttatcttc
     2521 ctaaaatgga tgatttgaaa caaacagcta agaaaatgag agaattccta gcaacctatc
     2581 gccaaaaatg attttaaaaa ccaaattttt cagtaggagg gccacagaca aacacactga
     2641 ggctctcttt ctagtggagt tttcttaaat aactaaagta tattatttag ttgcaagtct
     2701 tttcttgaag tttgcgactt cctaaagtaa aatacgaata gaatgcgaat acgcatattt
     2761 tggggaggat acttatggaa acgttaatcg agtcctttgg aacgatcatt aaagagattc
     2821 gaaaagaaca aaaaatgacg caaaaaatgc tttcgcatga tatttgttca caaagtgtat
     2881 taagtcggat cgaaaataat gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc
     2941 aacggctagg ggtaacgatt gatcatgtga tgaatctctc tcgaaaaact actcggacaa
     3001 acgataaatt atttgaactg ttggatagtt gtttccaaaa acgtgattat cggaagttag
     3061 aacaagtact aaaatcttct gatatttctg aaaatctgta tcaagcaact gactttcaac
     3121 gctattatta ttatctcggt gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc
     3181 tttattattt gaaagaagca ttaagctact ctagccaaaa agaaagaatc catgtttctg
     3241 acacagaaat acaactgatt agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg
     3301 ctgaagcacg ctactatttg agtcgaagca tccaactgct gcatcaatca gctgatgaac
     3361 gatcaaaaag tgaattttcg caaatttttt ataattatgg aaatttctta ttccatcaaa
     3421 atgaaatcga cgaagcgtta gaacaggtga atcaaggaat ctattgggct caagagaaga
     3481 atagctacta ttacctcaat gatttatttg tgcttaaaag cttaatatac aagcgtaaag
     3541 aagaaccaga aagagcagca ttctatgaag gcttagcaca agctgtgaaa caaatttcaa
     3601 aaaatttgta aatggtgtag accattcttg acttcttctc taaataagag tactttagag
     3661 gtagaaaaac aaagggagag aatagaatga gaacttttat ctatgctgac aaattctttt
     3721 tagcgtcagg tgtcaaacat gcaggctatc ttgaaattac cgatggactt tttggtgaat
     3781 acaccaaaga actacctgag ggagaagtca acatcattga ccaaagcggc aaatggattg
     3841 ctcctggttt ggtagataca catattcacg gctatatgaa tcatgatgta atggataatg
     3901 atgcagaagg catcaaagtg atgtctgaag gtctgttatc ttgtggagtg acttcatttt
     3961 taccaacaac attgacgtca agcaaagaac gtttaaaaga tgtagctgag acaatcggca
     4021 aaatgtatca ggaagcacca ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct
     4081 tcacagaaga acacaaaggg gcacaaaacc caagttattt cggggatcct gatttggata
     4141 ctttccatga atggcaagaa gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg
     4201 aacgtcaagg tgtagaagag tttgtcaaac aagtgactga agaaggagta gttgtttctc
     4261 taggacacag taatgctaca ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg
     4321 tatttgtcca tgctttcaat ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg
     4381 gtgcgttatt atctttagaa catgtctttt ctgaattgat ttgtgatggc catcatgtac
     4441 atccaaatgc tgcgggaatc ctaatggaaa aagcaggaca tgatcatgtc gcattaatta
     4501 cagactgtat gatggccggt gggatgcctg atggaaatta caatttaggc gaatttccag
     4561 tagtagtaaa agaaggaaca gcacgtctag aatctggcaa cttagctgga agtatattga
     4621 aattgaaaga agcaattaaa aatgttgtag actggggatt agcaacacca gaacaagcag
     4681 tcatgatggc tagtctgatt ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa
     4741 tcgctcaagg acgcgatgca gactttatcg tattgaaccc tgatatgaca ttggaagcaa
     4801 cttatctaga tggcgtggaa cgtttccacg cataatataa tttaagttaa ccttaaaact
     4861 aaacttctta agtttggttt taaggttttt ttgtgttcta gtaaaaatca ctttacataa
     4921 gtttcaaaag tttctttaat ttcctattgt tcaataagta acaatgtaaa gataaaatta
     4981 aaaatgttaa tacgtattag caaaataaaa gcttttttta ttgtgaataa atgaacataa
     5041 ttaggaggag aaagatgaac ttatttgaca tttttcgatc gactttgaca gatatgtcga
     5101 ttgtcagtgc aatcacttca acagttttta ttatattatt agggtttgtt tgtagaaaaa
     5161 aggggatttt ttctgaatct gttggaaaaa ttctatcgaa agtcgtctta acagtggctt
     5221 tgccagcact ggcatttcga gctttcatgc aagatatcaa tacagaatta ctgatgcaag
     5281 gaatcaatgt cttgatttgg ggattagtta tttatattat tcttattcta gtttcaaaac
     5341 cttttttctt aagctataaa ggggataagc aaacaacgtt gcgtgtcctg acgatctttg
     5401 gttctacgac ctttttcgga acgcctatcg tcagtgcgat ttatggtcct gtcggcgtga
     5461 tgtattcctc aattttcaat attggctacc gaatcttttt gtattcttat ggatacataa
     5521 aaatgagtgg cttgaagatg gaagctaaaa atatcaaagc gatgttcgtc aatccaatca
     5581 ttatcgctac ttttgccggc cttttcattt ggctgttcca agggtatctg ccacaagtgt
     5641 cagttacagg tgcagaagga gagataagcc aagtagcgtt tttgcgaatt gaccagactg
     5701 caccgtggct atttcagccg atggattatt tagctggttt aagttcacca ttggcgtggc
     5761 tagcaatcgg cgcaacatta ggtgaagtaa gcttcaaaaa agctgcatct gaaaaaactt
     5821 catggtatta cagtctagtc aaagtggtat ttgtacctgc attgaatatc gtgattctag
     5881 cagttttgac ggcaacgaat attctaccag tcaattttga agcactggca acaatcgtta
     5941 tcatgatggc tacaccagct gcaacagtgg cagcagccta tgctatcagt tttgataggg
     6001 aggcactttt agcctccaat gcatccttac tgtcaactat ttttgctgtg gtactgacac
     6061 ctgtatggat cgttgtatta caaatgattc atggcatagg tttattttca taagaaatgg
     6121 aggaagaaag tatgagaatc gcatgttacg gtgttcgtcc gaacgagata gatttttttg
     6181 aaaaattgaa tatttaccat tatgatctca gtctttatga agaactgttg acacatgaca
     6241 atatcgtgtc agcaaaggac catgatgctg tattgttgag gggaaattgt atagcagatg
     6301 agaccaatct tgcaaaaatg caggaatatg ggattcgtta tgtttttaca cggactgtgg
     6361 gagtcaatca tatcgatcta caagcggcag ctgattttgg tatgacagta gcccgtgtac
     6421 catcgtattc gccaaatgcg atagcagagt tatctttgac ttttgccatg atgcttttga
     6481 gaaatactgc gtatacgacg atccgaacat ctttcaaaga ttttcgagtg gatgagcaaa
     6541 tgtttagtcg tgaaatcaga aattgcacag tagggatcat aggaacaggc aggatagggt
     6601 tgacggaagc tgttttgttt aagggactag gagccaaagt gatcggctac gatattcatc
     6661 agacagaagc tgctaaaaaa gtattgacgt tccactcgct tgaagagtta ttagccaaaa
     6721 gcgatattgt cagtattcat gtacctcata tcccaggtga aaacgatcaa atgatcaatg
     6781 aaggattttt agctcaaatg aaaaaaggta gcatcttgat taatacagcc cgtggagagc
     6841 ttcaagataa tgaagctatt ttaagagctt tgaaaagcaa tcatttagct ggttttgcaa
     6901 ccgatgtttt tgcaaatgaa acagaggttt ttttccgatc cttcaagcca tgggagacga
     6961 ttccagaccc tgctatccag caattagtag aaatgtaccc tagagttctt gtaaccccgc
     7021 atgtaggttc aaatacagat gaggcattat caaatatgat ttcgacaagt ttcgagaact
     7081 ttcgtgaaat aatagaaact ggaaaaacga aaaatgaggt atctcttcct aaagcccgtc
     7141 aattaagata agaaaacatg gatattcgtc aataaaatgt ttaaaaatag tccaagtaaa
     7201 cagttgttac ttctgtatac ttggactatt ttgtatggga taattcgatg actcaacagt
     7261 aatctgtgag ctttttccgc ccattctttg tcaattcata acaggttagc ggctggccga
     7321 tttccaaata tttgattctt tcgttcaata tatcttgttc tactaaaaaa ttcaaatatt
     7381 tcttgacgga tatacgtgac agtgagcttt tttgagccag ttcttttgtt gaaaaaatag
     7441 tatcttcttt tagaataaat gcacttattt tttttaaagt cagttttgac agacctttag
     7501 gcaaatcgat gaatgatggt atttcgtgaa tgggagtggt gtggttgaat acttgatcga
     7561 tcaatttctg atcggctttt tcagtcgttt ggattgtctg agagaaagca agatactttt
     7621 cgatcgccag ctgaaaacgt tgcaaagtga acggtttgat caaataatct aaggcaccgt
     7681 agcggatcac attctttatg gtttcgatat catctgcagc agtgatcaaa atcactggga
     7741 tatcatttcc ttgctgtcta agccaagata aaaaatggat accactttcg ccaggcatgt
     7801 atacatcaag cagcagcaga tctgcgtttt tttgtctgaa atattttttt ccttcttcta
     7861 catttctgaa actttcgatt acgttcatat tagggatgct ttccacaaaa gtacgattca
     7921 attctgcaac cattggatca tcttcgatta tcaggacatt tgtcatcatt ttgatcttcc
     7981 ttttttggta attctagata gacgattgta ccgccatttg tttgtttgga aaattctaaa
     8041 aatccaccgt ggcgtttgac gattgaatgg atgagattca atccaaaccc acgattgatt
     8101 cccttcgtcg aaaaacctag cgtaaatatt ttttctgaga tttcttctga tatcccgaca
     8161 ccgccatcgc agacggtcac gatgaatact tcagcttctg tgtcatagtt taaatgaatc
     8221 cggacctgtc cgtttctttc atgtttttgt agcacggctt cttttgcatt atcaagtaag
     8281 ttccctaaca cttgcaaaag tgaatggatg gagtcatcca tctccatttt agggataaaa
     8341 gaatcgcttt ctaacagcat ttcaacagat tgttctttgg cttcgtttat tttgcctaat
     8401 aaaaaaccgg caattgcagg tactttgatc agacttgaga cttggccaat ctctttttta
     8461 taattttgat ttagctgctg gataaaatgg gtgacttctt catacttctt caaatcaatc
     8521 aaaccagaaa tgacatgaag tttattcatg aattcatggg tttgagctct catggagttt
     8581 atatattgtt tcgtcccccc aagctcctcc attaatttca ttacttctga tcgatttcgt
     8641 aaagttgcga ctgctcctaa aaaacggtca ttgctaaaaa gaggtgtaac gctagaaata
     8701 gtttctatcc catttaaata catggattga gtccgaacgg tcgtttgatg ctcaatcgtc
     8761 tctttaaaca aaacgtcata tagattatca ctgatttttt tcctacttcg aaatccaata
     8821 gctgctctgt ttggtagaaa atgttttttg caggtgtatt gatcaagagg atagttttat
     8881 cagaagagat ggctaagatt ccttcatcta cttcattttc gataatcgtt ttttcactta
     8941 actgttctgc tatttcttgt ggttcaagtc caagcagtac ttttttcatt ttttgggcaa
     9001 gaaaaacggc gcctatgatg cctacgctaa gtccaataat caatgaaaaa acaatcattt
     9061 tttgtccatc tttgacttga ttgtcaatcg tgttgtttgt taagccaaca caaactgcac
     9121 caataatctc ttgatcataa taaataggga cgaaatagcg gaagccgaac cctaacattc
     9181 cttctttttc agagaaaaat tcttgtcctc caatcacttt tttcacatct tcatgacttg
     9241 agaaaggaag accgatgtaa cttgggtctg cgtgagaata tcgaatgcct tcagggtcca
     9301 tgacaacgat aaaatctaaa ttcgatgcaa tcatgatttc atttgttatt ccttgaatcg
     9361 catgtgtcgg gtcaccagtc tgcagctttt tttggatgag tttatcattg gcaacgattt
     9421 tagcaatgct agagatttta tctgacacac tagatcggtt catcgaaaga ataaaattcc
     9481 tgatcaatat ggcagaaaca atcaaagaaa cgaatgtgga gaaacaaacg acaaggatga
     9541 tcgtgttctg gagctttaat ggttttttct taaaaaaagg aaacataggc tacctcgaaa
     9601 gaatttattg tgaaaatttt aacatgaaaa caaaaattta acaaattgtc aacgataaac
     9661 acaaccttcc tataaaaaac accactgagt cggtaaaaga agctatacag aattttcaaa
     9721 cattggtatg atagacgtag aaatggaagg aggcgtgtat atgatagggg gaatcgcact
     9781 tgagacgatt tattggtata cattgctgat ttgtgcagga ttggcagtcc tattgattat
     9841 ttttggagat gtattcgatt ttgatggccc gcttgatccg atgcttatta tcccttggtt
     9901 aactttcaca agcttatttg gttttctagg tgagcgttta acagatcaat catctttatt
     9961 tttatttatt gctagtggag gcatatcgat agtgttggta tttttgctta atttctatat
    10021 attgatgccg atgaaacatg ccgaatcaac gatgtcgtct tctgaaaaaa ctctggaagg
    10081 acaagtagct gttgtgatca caccgatccc tgtacgaggt atgggagaaa ttcagttgaa
    10141 aagtgttacc ggttccatta gtcgtccagc ttgtttttac agcccccaag aacagacagc
    10201 tgagcgggga acgaaagtat tgatcattga gatcaaggaa agaatctgtt atgtcatgcc
    10261 ttatgaaagc atgataaaag cttaaaggag cgtgtgttaa atggaaatac cagttgtttt
    10321 tatcgcgatc gcaatcgcag ctttgatttt actgatgttg ctgatcgtct ttgtagcgaa
    10381 gtaccagaca gcaaaaccgg atgaagcgtt gatcatcagt ggaagttatt taggaagcaa
    10441 aaatgtgcac attgatgaaa gcaataacaa aatcaaaatt gtgcgtggag gtggcgcatt
    10501 cgtcttgcca gtttttcaac gttcgaatcg gatcagtcta ttatcgagta aattggacgt
    10561 ctccacccct gaagtgtata ccgaacaagg cgtaccggtg atgtgtgatg gtacttcgat
    10621 catcaaaatc ggttcatctg tagaagagat tgctacagca gcagaacagt ttttaggcaa
    10681 gacaagagaa gaactggaaa atgaagcaag agaagtatta gaaggacact tgcgctcgat
    10741 tcttggttct atgactgtag aagaaatcta tcaaaatcgc gataagttca gtcaaagcgt
    10801 acaagaagtc gcttctgtag accttgcaaa aatggggttg atcatcgttt ctttcactat
    10861 caaagaggtc agagacaaaa acggttatct tgactcttta gggaaaccaa gaatcgccca
    10921 agtcaaaaga gatgcagata tagcagaagc ggaagctcta aaagaaacgc gtatcaaaaa
    10981 agctcaagca gaaaaagaat cacaagcagc tgaattacag cgacaaacag agatcgcaga
    11041 atcattaaaa gaaaaagaac tgaaattagc aacctacaaa caagaacaag atgttgctaa
    11101 agcaaaagca gaccaagcct acaatttaga aagtgcccga gcacaacaac aagtaatcga
    11161 acaggaaatg cagattaaaa tcgtagaacg ccaaaaacaa atcgaactgg aagaaaaaga
    11221 aatcacacgt cgtgaaaagc aatatgattc ggaagtgaag aaaaaagcag atgccgatcg
    11281 gtacgctaaa gaacaagaag cacaagctca aaaagtcaaa gaagtgactg aagccgaagc
    11341 agaacgattc cgtgtggaag cactggcaga agcggaagcc aataagacac gtttagctgg
    11401 acaagcagaa gccgaagcag cactagcaaa aggaaaagca gaagccgaag caaaacaaaa
    11461 gatcgccaat gccttcaagg aatatggcga agcagctgtc ttgagtatgg ttatcgatat
    11521 gctaccgcaa ttgatgcgcg aagcagcaca gccgctagga aatatcgaaa aaatctctgt
    11581 tgtcgataca ggaagcagtt caggagaaac cagtggagcg aaccgagtga caaattatgc
    11641 cacaaatttg ctttcttcta ctcaagaaac actaaaagcg acaacaggac ttgacttgaa
    11701 ggaaatcgta gagaatttgt cgacacgtgg gactactagc caagtaaata tgtatgaagc
    11761 gccaaaagag gcagaacaaa actcgatgaa ggaagaataa agtagtacac ttcaccgttc
    11821 aatcaaaaat ggaggaagac tttttaaagt tcttctctcc atttttttat tttattgcaa
    11881 aagaaaatta gtcctcgcta tttttctttt gtttttggat cttagcttct atctcttgta
    11941 aatttgttct tagcaatcgt tctttctttt ggagttcgac caaatcttct gatagttctt
    12001 ccttcttttc tgtttcagga ggacgaagaa agaaattgat accgattgca atgaatgtcc
    12061 ctataaaagt atctagtaca cgttggatag catacaaagc ggattcgcct tgagggatgc
    12121 tcaacgcaat cagcaacaac gtagctattg cggaaatgat accggaatta ttgttaatac
    12181 catctgacac gacgatcaca ataataacta gaataggaag caagatcaat tctaccaaaa
    12241 aatcattgtg aaaatagctt ttgacaaaaa aataaatgat agctaatgta ccacctaatg
    12301 tattgccgat gatcctagac tttccaaaag aaacggttgt agtcaagtcc tgacgcaaag
    12361 aaaaaacagc agataatgca gcaatcatcg ggattcctcg ttgagtcaaa tgaaaaaaca
    12421 agatacataa aaaaacagct aaggcacttt tgatcgtacg catacctagt cgaaaacggc
    12481 caaattgcat agagattctc ctttttggtc tttttactat catacctaaa cttttgtctg
    12541 atagctagtt tttcattatt caagtataat taaaataaaa aagggggaag taaatgcgta
    12601 taggaatgcg aacagtcaaa acagtgatca gtgtcttctt ttcgatggtc acagcgggta
    12661 gcttatcgct gttgtattgg ccagctgcag ggatcattgc attattaagt gtaggaaata
    12721 ccaagcgctc tacattgcgt acgggcatga atcgaatcgc agcctttata ttagctacgg
    12781 caacagcact gttcagtttt tttgctgttg ggtatactat ttttggcttc agtctttttt
    12841 tattcctgtt catcccgctt gcctctcgct tcaaattaac cgaagggata gtagtaaatt
    12901 cggttttggt cactcattat ttagtggagg aaaacatgtc ttggcagctg atcggcaatg
    12961 aagcgggatt aatggcgatt ggattagtat ttgctttgct tgcaaatgtt tacatgccag
    13021 atcgtacgaa gcagctgaag gaaaaccaaa tacagatcga aaaagaattt cgaaatattt
    13081 tgagacaaat ggcagaattt cttttatcag aaaataaaga agatgtgcag atcaaatgcg
    13141 aacatttact gacattcatt cgcgaaagcc aagaagatgc cagagaacat caagaaaact
    13201 actggctcag acagccgcta tactacgaaa cttacttctc tatgagaaga gcacaggcca
    13261 atgtgattaa agacatgctg gaaaatctag agagaatcca gcaaccagct tattatggaa
    13321 aacatatcta tggtttattg atttatactg cagaaacatt ttcagagtca aatgatggca
    13381 gacaaatttt aacacgtatt gaagaagttt atgtattgta tcggcaaatg cctctgccga
    13441 ctagtcgccc tgaattcgag gatcgagcag aattgttcca gtttttgcag tcttttaaaa
    13501 gttttattga aatcaaggca gaattctccc aacaaaaaat ccaaaaatag aggttgtgaa
    13561 ttcagctaat attcaggtca aaaagctgat ttcacaacct cttctttaaa cggtgttctc
    13621 ctgtcaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa
    13681 attgctcctt gtgactcgga gctgaacggt aggctcacaa cctctttctt aaacggtgtt
    13741 caaaagcttt cctctgcggt aataagtcca gccaaacgaa aaataggagg agctattttt
    13801 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctttt aaaattacat
    13861 atttttccat agaacttgca tttgatccaa gatttcgatg acatcttttg tcaagaagag
    13921 agagcgattg ggcaagtcac cttcaatcat gtgtaccata ttctttattt cgtaattcat
    13981 tgcttcagat gtattcccgc tttcgatgaa ttcctttgta ccatctgtaa ataagatctc
    14041 cgcacgatca gcacgcgggt aatcagcaat cgtcacataa ccgttctcaa aggcaacgat
    14101 accgactttt ggcatttttg cctgaaaagt caagctgaca gcagcaagtt cattttcttt
    14161 gttacgcaag atagtgacgg attgttcgtc cacgcctgtt tcaaaaggaa ccattgtaga
    14221 ggcaactact tcaggttgag agctcaaaaa gaaacgagcg aaagatacag catacgtacc
    14281 gatatccaac agagctcctc cagctaattc tggattgaag aaccggtttt tagggtctgg
    14341 ttctttgtaa ctgccgaaag gtgcttggat catttttaaa gcccccagtt ttcctgtatc
    14401 cattattgag cgtaactgct gataaagtgg catgttgaag attgtcatcg cttctgctaa
    14461 gatcaaattc ttttcttctg ctaagcgcat agcgtcagcc agttcttttt tattcatcgt
    14521 gatggctttt tcacatagaa cgtgtttgcc tgcttccaat gctgccaaga tatggtcgat
    14581 atgctgtcta tttggtacag caatgtacac gatgtcgatt tcagcatcgt ctaacatttc
    14641 ttgatagctt ccgtaagctt ttggaatatt gtaacggtga gcgaagtttt ccgctttttc
    14701 tgaagttctg gaagcaactg ctgttagttc gctcgtctct tgattgaagc tttcggcaaa
    14761 ctcatgtgcg atggtaccaa gaccaacgat tccccattga tattttttca tctgaaaacg
    14821 ctccttcata gttggattga gtttcttgtc tgtctattat tataacaaaa atcacgaaga
    14881 ataaagagga gaggacttga aacattaaaa aaaatgaaag cagcgattta agaggtgctt
    14941 tttgatcaaa acttaagtag aatagttaaa aggaggggat aaacgtgcat caagtatata
    15001 ttgatcttat tatgagtgta ttattagaac agtttgaaac tgaaaaaaat tttttagatg
    15061 aatacttgct gatcagtcca gaacaatggc aaaaatggaa agatggcgag aaaaatctgc
    15121 catctgaagc gatgcagaaa gtaaaaaatc ttttcactga ttatgaatgg atgctaacgc
    15181 aaaagatttt gcgtcaaacg attatttttc ccgaaaaacg aaacgttgcc gttgcagagt
    15241 acaagcaaat caaaacacgc atcgcgcaaa aatggcttag cagcaaagca gcaaaagtcg
    15301 agctgatccc tttgaaaaat aatcagcagg ctggtagata ccttgacttg aaagtaagta
    15361 ttcaatatga tgaatggggc tatgatgata ttttgaattt tcgtctgcca gcagatgttc
    15421 aaaaacagat cgaaggagaa aaaatcgaac ttctcgattg ggtgaatgaa aatctggaag
    15481 aaacttacgt tcatgaggcg aatggggaac gtgaagaaca acgaaaggat ttttcaagtg
    15541 aataaatttt taggatttgg agcagttgtg attatgatcg cagcaatggg ctatgttatt
    15601 tttagcgatg atgattcgca tgttcaagcc ggcaatcaag cagtcgaacg aacaagcacc
    15661 aaaacaactg ctagtaaaga aaagaacagt caaacaacca gcagcaaggt aaaggacaaa
    15721 gaaacaaaag atcgtacgtc caaacaattg ccagatgtta aaacagacga ttggaagcta
    15781 gttttggttg gtccagatca caaattagaa aaagaaattg atgagtctac tcaattggca
    15841 tcattaagta acggatactt gatcgacaaa cgcattgctc cagaatatga agcatttgcc
    15901 aaggctgctg aagcagctgg atttccatta gtgatggtct cagcttaccg gtctgtatca
    15961 tcccaacaac aagttttcga acaaaatgtc caagatgtga tgagtcgtca aggtgtatca
    16021 gaagaggagg cgacaaagat tactaaagaa acgattacgg agccaggata cagcgaacac
    16081 cacacaggtc tggcagtcga tgtcgtcgat caaaactggt acaacagtta tccttctact
    16141 gtattggatg caagttacgg agatcagcct ggagctaaat ggatcgcaga taatgctgcg
    16201 cagtatggat ttatcgttcg ttatccaaaa ggacacgaag atataactaa gatcacatat
    16261 gaaccttggc acttacgtta tgtcggtaaa gaaaacgctg agtatatcac caaacaccaa
    16321 ttgacgctag aagaatatct gaatgaatta aatgaaaagt aggaattgat catggcgaaa
    16381 aaaaagtgga aaaagcaaaa gcgcaagaat atccgttggc cagcagtggt tgccggtgta
    16441 gcagtgatac tcttagcctt tgttttttcc ttgaagaatc tgtcttctcc ttttactgat
    16501 aatcagcaag aaaatgaagc gcaatcccat cagcaattta ttgaacggct tgttccgcat
    16561 gcccaagaac tacaagatgg ttatggagtg cttcctagta tcattttagg acaagctata
    16621 ttagaatcaa attggggaaa aagtaccttg gctagcaaat acaataatct tttcgggatc
    16681 aaggcttatg gggatcagaa gaaagtaagc ctggaaacaa aggaatttgt taacgaagaa
    16741 tggataacta tccaaggaga cttcaaagtg tatgattcat gggaacagtc catggatgac
    16801 catacacaac tgttcgtcca aggagtagat tggaatcctg cgctgtatga aaaagtgatt
    16861 acggccacca actatcagga agccgcacaa gcattgcaag atgcaggtta tgcgacagac
    16921 cccggttatg cgcaaaaaat catccaggtc atcgagacgt atcaattgaa tcagtatgac
    16981 cggagatagc aaaggagaag tggctgatgg cagaacaatt tacgccagaa atcatgacgg
    17041 aattgagaaa agatatcgta agagtaccgg aaatcatttt agaagcaagc gggatcaaga
    17101 tatttggaaa attgatccgt tcgatcatct ttactacgga tattgcgatc atacgtaata
    17161 caaatgcgaa tgcagtaatt gctgtgtatc cttttacacc tcatccagcg attactaaaa
    17221 gcattattga ggcagctgac attccagttt tctccggtgt aggtggtggt ttgacacaag
    17281 gtgctcgttc ttcctacatg agcatgttcg cagaagcgca aggttcgtta ggggtcgttt
    17341 tgaacggtcc tacgccttta gagacagtaa aagaagtttg tcgagtagtt gatattccag
    17401 ttatcagtac ggtaacgtcg aaatatacag aaatagatga aaagttagca gctggtattt
    17461 cgattatcaa tatcagtgca ggtaaagaaa ctgcagaagc agtacgccat tttcgtaaaa
    17521 aatatcctga acttcctatc attgcgacag gtggtccaac ggaagaaagt attgctgaga
    17581 ccattcaagc aggagcaaat gccatcacat atacgccacc aagtagcgga gagcttttta
    17641 gtcgtaaaat ggaaaaatat cgggaaactg agaaaaagaa tcatgaataa caggtgagaa
    17701 tcaaaagacg aggaagagca gatagtggct cttcctcgtc ttttgtactc cagtactatt
    17761 tgttcagaat tcaaaaaaaa gagaaatttt tttgtttttt tacctgtaat gtacataaga
    17821 agagttaaaa ccttttgtta ttattagcat tctaacaaaa aaatgtcttg atttattgaa
    17881 gcgctttcta tagaatgaaa aacgaataaa aactaaaaaa ggcacccgac aatttagtag
    17941 cagctaaatt gtcggggctt gaaaagtcag cgggaactga ccaatcaagt tgccatagtc
    18001 aatgcttgtg catcgacgtg tatattgtac ctaatttcat acagaattac tagttatttt
    18061 tctgtttttt agggaagagc gcacacgaag aagcatgctt atttagctat gtcaaggata
    18121 ttgggcagtt gtctgatatc cttttttctt tgcgtaatta tcctcccatc attgaagcaa
    18181 gaagtgtttt tagcgatgaa agaagaatca gcactatcta atttttcttg aaagtattga
    18241 gttgttcatc ctaggcagaa cgataaggca agtcttggtt tttattcccc ttgaaaaaga
    18301 tcgtttaata aatatgttct gtataaaaat acagtatatt gaaaaataac aatggaagta
    18361 gaggtgcaga gaatgaagaa aagaatgaga atcatcttag ctttaagtgt tatgttattc
    18421 ttcttgacgg cttgtgggca agaagatgac aaaaaaatca cgctgaacat ggcttggtgg
    18481 ggttcgcaag tccgacacga tgcaacagta aaagtaatcg agctttatga agaaaaaaat
    18541 ccccatgtga cgatcgatta tgaattttat gattatgaag gatattacac aaaattaggt
    18601 acactagcag catcagatga tatctgggac attttccaac tgaacaatca atttcctcaa
    18661 tacatcaatc agattgaacc attagatgaa tatgtggagc aagggataat tgatatttct
    18721 aatgcagatg aatcctattt agcaacgacg acttacgacg ggcaattagt tggactgtct
    18781 aacggggtga attcgtttgc gattgcttac aataaaaata tatttgaaca attaaatatc
    18841 cctgaaccag cttcaaattg gacatgggat gaatttgaac aaatcagcta tcaaatcact
    18901 gaagaattag gaatctatgc tatttcaaga ttcgaagatt ttatagctgg ctgtattatt
    18961 caaataccac aagttgagaa aggattgaat ttttataatc gggaagatct atctaacagc
    19021 ttgggatttg aaaatccaga ctatctaaca gattttttcc agctgcgtaa aaatctggta
    19081 gacaaagggg cccatccaga accaggtggt ggagctgcaa gtacgacgga tctgtctttt
    19141 caggcggtta gggatagaga agcggcaatg gtgtttctat caagtaacca gttgacagaa
    19201 atattgtcag gggctcctga agatatggag attcagttga ttaatccacc tcgcagaaaa
    19261 gcagatggag aatctggtat ccctttacgt tcttcgcaaa tgctgagtat ggcaaaaagc
    19321 tcagcccata aagaagaagc tgctaagttt attgatttct ttcaaaacag tgaagaagcc
    19381 aatgaaattt taaaaggaga acgtggtgta ccaatcatgt cgaatattcg agaacagctc
    19441 gctcgtgcag atgatgcaac tttgcaagcg acatttgatt atttagaaat gatttccgat
    19501 atggacaacg gcgaagtgaa tgtctttgat agtgctttga atccggaaat tcaagaccaa
    19561 tacaatcttt tagttgaaaa agtcatttat gaagaagtaa cagcagaagg aaggcatgaa
    19621 aaaagaaaaa ctaaaacggt tcttggatca agacaatgtg gtcggttacg tatttgcttc
    19681 tccattcatc ctaggatttc tattgataac agtgataccg atgatcttgt cactttatta
    19741 ttcgttcacc gattatcgtt tgggaaatcc agtctcatgg atcggcttgg ataattatat
    19801 aaagatgctg agtgatgccc gttttttgaa ctccatccga gtcacttttc tctatgtatt
    19861 tgcatctgta cctgtgaaac tgatctttgg tctattgatt gcttatcttt taacgaggaa
    19921 tatcaaagga acgactttct ttcgttcttt gtattatgtg cctagtttga tcggtggcag
    19981 tatttctatc gctctagttt ggcgggagtt gttttctaaa aacgggttag tgaatatttt
    20041 cctggaacga ttaggcgcac ctgcgctttc ctggtttagc gatcaaaaac tggtcatgat
    20101 ccctcttgtt ttgatgtctg cttggcaatt cggttcggca atgatcatct ttgctgcagg
    20161 tatcaaagag atcccaggct cgtattatga agcggcagag atcgatggag cgaacaaatg
    20221 gcagacgttt tacaagatca cgttacctct tctttcgccg atcattttgt ataacttggt
    20281 tatgcagaca atcgcggcgt ttatgacttt tacgcaggca tttatcatta cccaaggtgg
    20341 tccgaatgat gccacgaact tttatgctct ttatgtgtat aaccaagctt tttcttttaa
    20401 ttctatgggg tatgctagtg ctatttcgtg gatcatgctg ttgattatgg gagctgtcac
    20461 ctttctgatt ttcaaatcat caaaaaaatg ggtcgttgtg tgaatgagag gaggaagaaa
    20521 tgaataatac agtaacgaaa tggtttcaat acggttttat tttgctatta ggtctagtca
    20581 tgatttatcc ggtcttatgg atgatcgcag gttcttttaa aacggatcaa gagattatga
    20641 gtggttcgct gaatttgatt ccagagactt ttcaatggga aaattacgca aaaggatggg
    20701 cggggtttgc tggaatttca ttttttactt tctttaggaa ttcgtttgtc atctctgcag
    20761 tttcgacgat agggacagtt ctgagctcga cttgtattgc ttacgcatta tcgagaatcg
    20821 atttcaaagg aaaaagattc tggttcgtcg tcatgttagt aacgatgatg attcctgcac
    20881 aagtgatttt gattccgcag tttattattt acaatcggct gaatttggtt gggacgtatg
    20941 tgccgctgat tttaccacat ttttttggac aagcattttt catttaccag atcatgcaat
    21001 tcatggtcag catccccaaa gagttagatg aatcagcgat tattgacgga tgcagcaagt
    21061 atagtgtctt taccaaaatt atttttcctt tgctgaaacc ttcgatcatc acgacgatca
    21121 tcatccagtt ttattggaaa tgggacgact tcatgggacc attgatttat ttaaacaagc
    21181 cgagatctta cacggtatcg attgccatca aattatttgc agacgctgga tctagtacga
    21241 cttacgcgag tatgtttgcg atgtctacgt tgtcgctgct accggttttt ctgattttct
    21301 tattcttcaa taaatatctt gtccagggga tcagtacaag cggtctgaag ggctgaatat
    21361 gaaaaagcca atagaagaga tgtttcttta caagctcaac tactatgtct gtcggatttt
    21421 tttaaccagt cttatcagta atgctctttt cgctttagtt ctaggatgtt tgttcacttt
    21481 aggattagtg ctgtctgctc gtgttgtcta tctgatttat tttttgatga tgtatccttc
    21541 ttattatcta tctgtccatt acttggcaga aaaaaaggta tgttggagga aagggaaaat
    21601 caaacggatg tgtttcatca ttacgttgtt tcagctgttt gtcattattt ttgcagttga
    21661 ttggtattta ttagtgggaa agattggagc ttggtatctg attccttttc tattgtgtct
    21721 gttgcttttt gtatcccata gtttttttag tacgctaatt gatatctgca aagaagataa
    21781 agcagatagt cttgtttatt tgatcaaaag gaatatctgg ctgttgtcaa aaaacgtcaa
    21841 ggggtttctg tggtatttat gccttttgta tggactgttc tggtgtatac agcctgattt
    21901 tttactcact gcattactgt atccagggct gccatttgtc acagtatttt gcttaaaaga
    21961 aaaggagaat gactttaaat gaagaaaaaa tatgtacaag tcggacttgg tgggagagcg
    22021 cgtttttttt atgaggcaat tgcctctacg tatcatcaaa cttgtgaact agtcggtttt
    22081 tgtgatgtat ctcaaacaag gatggattat gcaaatcgga tacttgtgaa caagtatgga
    22141 cagaaaaaag taaagacatg tatgccatca caatttgagc aactactgga tgacgtacgg
    22201 ccggctgaag tcattattac gtcagtagat cgaacgcatc atgattatat cattcgtgca
    22261 atgaaaaaag gatatgatgt tatttgcgaa aaacctctga ctatcgatga ggaaaaagct
    22321 caagaaataa taacggtcca aaaacagacg ggaagaagcc tgcgagtcac ttttaattat
    22381 cgttatgcac cgtatcacac gaagataaag gaactgatag aaagtgatgt gattggtgat
    22441 gtgttttcta tccacttcga atggctgtta aatacgcaac acggggcaga ttatttcaga
    22501 agatggcatc gaaataagca caatagcggc gggcttcttg ttcataaatc tacccatcat
    22561 tttgatctag tgaatttttg gttgaacagc cgtccccaaa aggtatttgc attcggtgga
    22621 ttaaactttt atgggatgca taatgcggag aaacgcggag tagaaatatt ttacagtcga
    22681 tgctatggaa gcgaactagc taaagctgat ccatttgcta tagacctgtc agccaatgag
    22741 gaattgcgag aaatgtattt agaagcagaa aaagacagtg gatatattcg tgaccgcagt
    22801 gtattttccg atgatatctc tattgaagat acactaggcg taacggtcaa atatgaaact
    22861 ggtgcgattt tgacgtattc gctaaacgcg tatatgccgt gggaaggatt caatgtttct
    22921 ttcaatggca gcaaaggacg gatcgaaatg aaagtgatcg aaaagtctta catcaatgca
    22981 ggtggagata aaaaggatga aggagcagcc gttcttcagt ccatccatgt ctatccgatg
    23041 ttcggtgagc catatgaggt aatcgtcgaa caagaagcgg gcggacatgg cggaggagat
    23101 ccgcgtttat tagatgatct atttggtgaa gaaaaagaag aagatccgtg gaatcgggca
    23161 gcaactcacg tagatggtat tttgtcgatt ttgacaggaa tcgcagcaaa tcattctatt
    23221 gcttcaggaa aagcagtagt gattgacgag cttgtcagct taaaagaata agaatagatt
    23281 tacggaaatt acttcccgta gtttataaaa tcttgcctta ttgccgatag gctgtctttt
    23341 gaacactatt tattcggaaa aaggggctat gacatgcttt ttgtcatgcc ttctttttat
    23401 gtgggctgct tttttagata ggagaaaata aaaataacta tttaaacccg aacaaaaagg
    23461 aataatgcat taaaaatcat tagattttac aaaaaaataa gtaaaccacg ttgtcagttt
    23521 cagaaagtag tgttagactg ttttcaatat ttgaaacaac attttttatt caaatatggc
    23581 aaactgcctt cacgtgcaaa acagttgcaa gaaaatgaat ttacactatt attagtttta
    23641 catacctact tatatatcgt cataatatgg atgacagttt ctagccagta ccgtaaatgc
    23701 tggactataa gtaaaagatt ggctatttca cccttctttt cttatgataa agtggagggt
    23761 gtttttcttt aacagaaaca tagtaggttt ttgcacaaaa gcggttattt cggtattaat
    23821 aaatgataaa ggagcaaata aagtgaaaga attagttgaa cgtattcaaa aagacggacg
    23881 tatattaggc gagggcgtat taaaagtcga cagcttcgtc actcaccaag tagaccctgt
    23941 attgatggaa caaatgggaa aacggtttgc agaagtattt aaagaacaag gaatcacgaa
    24001 agttgtgacc attgaagctt ctgggatcgc accagcttta tttgctgcaa gagaattaga
    24061 tgttccaatg atcttcgcta gaaaagcaaa aagtttgacg atggatgaag aattattgac
    24121 agcttctgtt tattctttta cgaaacaagt gacaagtacg atttctattt ctcgtaaatt
    24181 tttatcttca gaagacaaag tattgattat cgacgatttc ttggcaaacg gacaagcggc
    24241 aaaaggattg atcgaattat gccagcaagc tggagcgcaa gtagaaggaa tcgggatcgt
    24301 catcgaaaag tctttccaag atggacgtca gttacttgaa gaaatgggat tgagagtcgt
    24361 ttctcttgcg cgtattgctt ccttgacgaa tggacaagtt gagtttcttg aggaggacgc
    24421 ataatcgtgg aaaaagaaac acaaaatgga aaagctgcgg ttttagggct gcagcatctg
    24481 ctagcgatgt atgcaggagc agtagcggtg cctcttttga tcggtacagg actaggcttt
    24541 aatcaagaac agatgactta tttgatttca attgatatct ttatgtgtgg attagcgaca
    24601 ttattgcaat tgacagttaa ccgatttttc ggaattggct tgcctgtcgt tttaggatgt
    24661 gcgatccaag cagtagctcc actgatcttg atcggaacca atgaaggagt aggagctatc
    24721 tacggatcta ttatcgcttc tgggatcttt gttgtgttag tttctggtgt cttttctaaa
    24781 attaaaaaac tattccctcc tatagtaaca ggaacggtta tcaccgttat tggtttgact
    24841 ttgattcctg tagcaatcga gaaaatgggt ggaggtaatg caacagcagc tgattttggt
    24901 gacaaaacaa atctgctact tgcatttgtg acgattctcc taattgtagg cgtgcaaatg
    24961 ctggcaaaag ggttcattcg ttcaatcgcg gtattgattg gtttagtcgg aggaagcatc
    25021 ttagctgctt tcatgggaat gattgatctg agcgcgattg gagatgcgcc gttgttccat
    25081 gttccacaac ccttttattt tggtaaacca actttcgacg tatggtcgat tttactaatg
    25141 atcatcattt ccatggtcag catggtagaa tcaacaggtg tatattttgc acttggtgac
    25201 attactggga aaaatgttgg agaagaagaa ttgaaaaagg gatatcgtgc agaaggactt
    25261 gccgttatct taggcggtat tttcaataca ttcccttata ctggcttttc tcaaaatgtc
    25321 ggattggttc aactttcggg aatcaaaaca agaaaaccca tctatttctc tgcattcttc
    25381 ttgattgttt taggattatt ccctaaaatc ggtgcgttag cacaaatcat ccctgagccg
    25441 gtacttggtg gaggaatgtt agtcatgttc gggatggtgg cagtccaagg aatccgtatg
    25501 ctgttggaag ttgatttcag caatgataaa aatctattga ttgttgctgt ttctgttgga
    25561 tttgggctgg gtttcaatgt tacgccgaca ttattcagtc aaatgccgca aacattgcaa
    25621 atgttcactg gaaatggcat cgtaatgagt agcattacgg ctatcatatt gaatttgatc
    25681 tttaatggat tgaaaaaaga tgaagaaata ttggaagaag ccttcaagaa gtaatagagc
    25741 aaaagaaagg ttgtgaaatc agcgttttga cctgattgtt agagaaacaa acggaaaaat
    25801 agttcctcgt attttgccgt ttgtttgagc taataccgag ggtcagctga ttgaacaccg
    25861 tttatccata aaaaggaatg tgacctaact tttgtcacat tccttttttt catctttaat
    25921 aaacacgaac aataaaatgt ttttctttgt taacattcga aatttctttg acatcaaaaa
    25981 aagtgattgt taaaatatga atgtagaaaa actgggagga aaaacaatga cagcaaaaat
    26041 ctctgtgatt atggggagta cttcagattg ggaaacaatg aaagaagcat gtcaaatatt
    26101 ggatgaattc ggtgtgtctt atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt
    26161 gatgtttgaa tatgcacaaa acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc
    26221 agggggagcc gcgcatctgc cgggtatggt cgcatcaaaa acaactttgc cggttatcgg
    26281 tgtaccggtc caatcacgaa cattaaatgg tttggattcg cttctttcga ttgtccaaat
    26341 gcctggagga gtacctgtag ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg
    26401 actacttgcg atacagatgc tttccatgta tgatttagaa ttggaggcta aattggctga
    26461 aagacgttca atgttggcta aaactgttat agaaagtagt gatcaacttg gttaaacctt
    26521 tgttgcctgg ttcaacgatc gggatcgtcg gtggcggtca attaggtcgt atgatggcga
    26581 ttagtgcaaa agaaatgggc tttaaagtag gtgtgctgga tcctgtgagc gattgtccag
    26641 ccgcacaagt tgcagattgg cacattgaaa gtgtctatga tgacaccttt gcgctgaaag
    26701 agcttgccag aagaacagat gtcattacct atgaattcga aaacgtcagt gtagaggcgt
    26761 taaatgcgat tttacccatg tcatttattc ctcaagggac agacttgctt gcgattacgc
    26821 aagaccgcct gttagaaaaa tcttttttgg aaacgaataa catcgtgatt gctccttatg
    26881 caacgatcgt cagtccgacg gatatccaag atgcgattga cgggatcggc tatccttgtg
    26941 ttttgaaaac cactcgaggc ggctatgatg gcaaggggca gtatgttttg aaaagtcggg
    27001 cggatctagc tcctgccatg gaccttttaa gagaaggaac ctgtgtttta gaagcatgga
    27061 tcccgtttga aaaagagatt tcgatcatgg tggcaggaaa tggtcaagga gatttcacga
    27121 cttttccggt cgtggaaaac atccaccaca acaatattct tcatgaaacg attgctccag
    27181 ccgcgattga tcaggatgtg atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag
    27241 cagtgagcct ttcaggtgtt ttaggggtag aaatgttttt aacaaaaaca ggcgggctat
    27301 atgtgaatga attagctcct cgtcctcaca attctggaca ttattctatt gaagcttgtt
    27361 ctatgagtca gttcgatgcc catattcgag gtatttgtgg ttggccatta ggagaaaaag
    27421 tacgtttgat aagcaatgca gtaatggtca atgttttggg agaacaactg ctggaaagct
    27481 atcgcttgat tgagaataag ccagactggc aattccattt ctatggaaaa aaacaagcaa
    27541 aaaaaggtcg caagatggga catatcacta tcttgacaga agacatccag caaacgctgg
    27601 aagaaatcaa acaaacgaat atttgggatt agaggagaaa aaacatgtta gaacgttata
    27661 cacgccctga aatggggaat atctggacgg aagagaacaa ataccgtgcg tggctcgaag
    27721 tcgagatttt agcagatgaa gcttgggcag aacttggtga aatcccgcaa gaagatgtga
    27781 aaaaaattcg tgagcatgca tcatttgata tcgatcggat cttggaaatc gaaaaattaa
    27841 cacgccatga tgtcgttgcg tttacacgag cggtttctga aacattggga gaagaaagaa
    27901 aatgggtcca ttatggtcta acaagtacgg atgtcgtaga tactgcttat ggctatctca
    27961 tcaagcaagc aaatgatatt ttacgggaag acttgaaacg ttttacggaa atcattggcg
    28021 aaaaagcaaa agaacacaaa tatacagtga tgatgggtcg gacacacggt gtccatgctg
    28081 aaccaactac ttttggattg aaacttgcca catggtattc agaaatgaaa cggaacatcg
    28141 aacgatttga acatgcggca aaaggagtag aagcaggaaa aatctcagga gcagtgggaa
    28201 cgttcgcaaa tatcccgcca tttattgaag agtatgtatg taaacaattg gggatccgtc
    28261 cacaagaaat ctctactcaa gttctccctc gggatctaca tgctgaatat ttctcagcga
    28321 tggcgcttat tgctacaagt attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat
    28381 ccgaaacacg tgaagtagaa gaattttttg cgaaaggtca aaaaggttct tctgctatgc
    28441 cgcacaaaag gaatccgatc ggttcagaaa atatggctgg tctagccaga gtggtgcgcg
    28501 gacacatggt cactgcctat gaaaatgtca cattatggca tgaaagagat atttctcatt
    28561 cttcagcaga acgaatcatc ttgcctgata ccacgatttt attgaattac atgttaaatc
    28621 gttttggcaa tatcgttaaa aatctaacgg tctttcctga aaacatgaag cggaatatgt
    28681 ctgcaacatt tggtttgatt tatagccagc gcgttatgct gaaattgatc gataagggaa
    28741 tgacaagaga agaagcttat gatcttgttc agccaaaaac tgcgtacgct tgggacaacc
    28801 aagtgcagtt ccgcccgctg ttagaagaag atccaaccat cacatcggta ttatctaaag
    28861 aagaaataga cgatgccttt gattatcatc atcatttgaa aaacgttgac gtgatattca
    28921 aacgagtagg tttggaataa agaagagtga gttgcaaaat tcaattaaaa tagaatagca
    28981 aaaataacca aaaaagaagc agatccgtta gcggttctgc ttcttttagt ttattatatt
    29041 ttcttatcaa taataggaac ctgattttaa aagcggctac ctctttaccg tgcctcttta
    29101 catacaaaaa atagaggaat taagcgtaaa tggcttggct attataacta aaatgctggt
    29161 tatggagatg ttgcagcaag ttttcgagaa aagcgtattc catctcttgt ccattgaata
    29221 agatctggat acgataatga tttttgaaac gcttctgttc atgaatctga ctgattgaaa
    29281 gttcgccttt gtgttctgtc tgataaagaa aagtcctttt acggcattca gcacagcgct
    29341 tggttgctcg gcggattttt cggaatgtat aaaaactagt atctttattg cataaataaa
    29401 cgtctttgat cgacagcaaa tcattatata gacaggaacg ttcttcagct gttaattctg
    29461 ataaaaaaag ctgaccttgt tttattgcat cttccatcgg caagtgcctc ctcgaaccca
    29521 ttatccaaat ttttagttaa ttctaatata ccatcattaa gtataaatgt aaacgcttgt
    29581 tgatggtata agattttcgt ttctgataaa acacgaacat taaaagaatc taatgcctta
    29641 aatgtacata tttttgttga tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac
    29701 gaacaaaaaa cgatgtattt gcttttaatg taaataaaaa ggagagacgt acatggagaa
    29761 gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg atgagaactt
    29821 gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga aagaccagat
    29881 aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt atctgactca
    29941 aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat tagcaaaaaa
    30001 agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa
    30061 acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat tctactataa
    30121 ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct tacaacttgc
    30181 aaaaccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg aaattttgac
    30241 cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat ttggccggtc
    30301 gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat tatgggatga
    30361 acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg atttgatccc
    30421 agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg agcgagtatg
    30481 atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc tcaaggggag
    30541 gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat ccgaatagga
    30601 aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt gattgaaacg
    30661 atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga aatcaaaaaa
    30721 atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa attgtgacgc
    30781 agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg tacggcatga
    30841 tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct cctatggtga
    30901 ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag tgattcgttt
    30961 tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat tgacagaagc
    31021 aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt gcaaaactgt
    31081 accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg acgaaataat
    31141 ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga
    31201 actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg gcagcgtgga
    31261 aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga
    31321 acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg cttccatttt
    31381 aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga cagctgaaga
    31441 gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat atcgaatgat
    31501 cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt tttctgttat
    31561 gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat ttcctactag
    31621 tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg
    31681 actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg agccttatga
    31741 aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg gggctcgtcc
    31801 aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa cgaaatattt
    31861 actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca taccgactgt
    31921 aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca atgcaatgtg
    31981 cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg tcggtaattc
    32041 gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa cttttgcctc
    32101 agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag gcgatccttt
    32161 tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt cggattctct
    32221 aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg aaatggcttc
    32281 aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag aaactgggat
    32341 gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt gtgttaaatc
    32401 aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg cagtcactat
    32461 cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag aagtagcaaa
    32521 tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa tgaaagaacc
    32581 agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg aagaacctgg
    32641 acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt caatctacga
    32701 aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg atgctgctgt
    32761 gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca acgcgcgcta
    32821 tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg ctagaaatat
    32881 tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg gttctcctga
    32941 taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg ctgcgtgtga
    33001 aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa cggatggtca
    33061 agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc ataaacatat
    33121 cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta agacgtttgc
    33181 agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag gtgtgatccg
    33241 aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag ccatccaaaa
    33301 cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg cactggcaga
    33361 atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct ctgctcaatt
    33421 atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata agacacgatt
    33481 cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg aagcgataat
    33541 cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg
    33601 ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg cgggatcttc
    33661 ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca tagtcttcag
    33721 catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct taaggggcat
    33781 cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca gcagttaaca
    33841 ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag tgtggacaat
    33901 attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca taatggtaac
    33961 ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat cttccattct
    34021 aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac agatttttta
    34081 aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt attaatgaca
    34141 gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc aatcggacaa
    34201 atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt tattggggca
    34261 cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga aggaatccag
    34321 attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata tatctatttt
    34381 gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa aaatatggga
    34441 cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt acctaattct
    34501 tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga aatcggatta
    34561 gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct aagagagcaa
    34621 ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg
    34681 gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct tttgaaagaa
    34741 gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata tccatgtttt
    34801 tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa
    34861 atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg tctgatcgaa
    34921 tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta tttcaacgga
    34981 gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa
    35041 gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg caaatgcata
    35101 cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga tcaagaagca
    35161 tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg gcggttgttt
    35221 tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg atggcgtggg
    35281 gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga tcgactgcgt
    35341 agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt tcttagacta
    35401 cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag gtgtagcaga
    35461 tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa tgcctggtat
    35521 gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg aaaaatctca
    35581 attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag catctagcgg
    35641 gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc agcagttttc
    35701 tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc ttgagccaac
    35761 gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa aaggagctgc
    35821 acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag aatctatggc
    35881 tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt tacaaaaata
    35941 tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg gtatggtcct
    36001 ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag ctggcgaaaa
    36061 agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga
    36121 agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca agcgctagcc
    36181 gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg tgatcagcct
    36241 gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg tttttcacct
    36301 aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa
    36361 aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt attgctgaaa
    36421 aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt ccctggtttg
    36481 catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac aattcattat
    36541 atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag aattgaacag
    36601 gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg aatctatcca
    36661 gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga gcattgatca
    36721 gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa caaggagtag
    36781 aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc aaaacgcttt
    36841 cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc
    36901 ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg gaggcaatgg
    36961 aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa
    37021 cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat atcggtggtc
    37081 ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt gttgatccat
    37141 ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca ctttctttca
    37201 gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca ctgatcgctc
    37261 aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt acgtatgaac
    37321 gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt tatcagtccg
    37381 ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa gaactttctt
    37441 ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat gacgagccaa
    37501 cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct acgattttag
    37561 aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg atcatcgttt
    37621 tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt ctagaaatca
    37681 tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag aaaaacatcc
    37741 gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag
    37801 tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa gaagagtgga
    37861 aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagcg tttgcttgga
    37921 gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt cagacagtag
    37981 gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc
    38041 aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg gatgattcgg
    38101 ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga agcatcaaag
    38161 accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt actgatgttc
    38221 ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg
    38281 gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat acggtgtatt
    38341 gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc
    38401 gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg agtatcgttg
    38461 gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa ggattgaaaa
    38521 tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt gctaaacaat
    38581 taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt tacgaaaaag
    38641 cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac ggcttagcag
    38701 ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg gcagaacaaa
    38761 tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg
    38821 caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat ccgatggtca
    38881 tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca ggcggaatgg
    38941 gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct gtggaagaag
    39001 ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg ggtgttttat
    39061 atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg
    39121 gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg caagtaatcg
    39181 atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga tacaatgtag
    39241 gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg gttctaccag
    39301 attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat aacggcaaac
    39361 tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg atcaaagaag
    39421 cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca ttttatcgta
    39481 cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc agctgcttcc
    39541 atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag ccttttcata
    39601 aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat gaaaaaaatt
    39661 caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat gcgtatcaac
    39721 aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat tgattttttt
    39781 gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca
    39841 aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat cgttccaggt
    39901 gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag tttgacccgt
    39961 ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt ggtagaacca
    40021 gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg ttattttggc
    40081 gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta
    40141 gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca agggattcat
    40201 gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga ttattcccga
    40261 ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt tgaaggtgta
    40321 tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact agcagctaaa
    40381 tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc agccaatatg
    40441 caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa
    40501 attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct
    40561 taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa taacttgaaa
    40621 gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag agtatcattg
    40681 tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc gatattcttt
    40741 tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt cattataacc
    40801 aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt tttttgctaa
    40861 ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat
    40921 cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta aggacatatc
    40981 tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg gctgatattt
    41041 gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat aaactgtcga
    41101 acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg tgcaatgaac
    41161 catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct tagtcaagac
    41221 aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat
    41281 gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt caaagtaaaa
    41341 gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga taatttgttc
    41401 tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg caggtaattc
    41461 gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt gtcccttttc
    41521 taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat tttcttcaag
    41581 agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga tcacttgttg
    41641 attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt caaagtctgt
    41701 actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag agcgattgaa
    41761 agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca aaccgttcaa
    41821 gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg
    41881 caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac ttttaatcgg
    41941 tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat cgaaatcgtc
    42001 gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt cttttgaaaa
    42061 agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg gctgtaagtc
    42121 gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag tgattggata
    42181 ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt attcgtcttc
    42241 gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat agagggatca
    42301 tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt ttttgatcat
    42361 ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg atactgaaat
    42421 ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct gcagggatgc
    42481 caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat gcggtaatac
    42541 gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt tcaatcaatg
    42601 gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata tcgatcttat
    42661 agtcaagcat atggccgtat cttattttta agatgactag ataatcattg atatggctaa
    42721 tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg
    42781 aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc aaagaaataa
    42841 tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat acacgaactt
    42901 cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg tttagatctt
    42961 ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta
    43021 aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc tgattgatcg
    43081 gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa aaaatggtac
    43141 taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt gtcgtatttt
    43201 ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct gacagcagtt
    43261 gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa tcttttgcca
    43321 agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta cgtagcgata
    43381 catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct cgaatatcat
    43441 tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact tggccagaaa
    43501 tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt gcatctgctt
    43561 tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc
    43621 tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct
    43681 gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct ggttgggatg
    43741 ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt aggtaatgtt
    43801 tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata ccagccgaca
    43861 tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc gatcatatct
    43921 tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga tgtttctttt
    43981 atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac atcgatttcg
    44041 gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat ccgaataaaa
    44101 tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt tccatctttt
    44161 tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag cttggtcggt
    44221 gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt tgaatttgtg
    44281 aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact ggggccaatg
    44341 tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg
    44401 atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg caaaggagta
    44461 aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat ttgccgttcc
    44521 tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag cgcagtgata
    44581 attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga aaaaccaacg
    44641 actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt
    44701 acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat ctggatttgt
    44761 tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa
    44821 tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat
    44881 cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt atcaattgaa
    44941 atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc gctattttga
    45001 ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat agtacggggt
    45061 atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat gggaacaaca
    45121 tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga gaagataaag
    45181 tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat acacttgtga
    45241 ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa gaagcagata
    45301 tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat tattacgcga
    45361 cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag cacttaggat
    45421 tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct gacgacaaca
    45481 acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg gacaacggtc
    45541 agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt cgctgggatg
    45601 gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact gaccaaacat
    45661 tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg tcattaaaag
    45721 gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa gatgcgacag
    45781 ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt aaagacacac
    45841 gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga gaagaggttc
    45901 cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg tatttagcaa
    45961 atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc gattattata
    46021 aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga tctaagtcag
    46081 agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca ccaaaaaatt
    46141 ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc
    46201 attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga gcggcttgta
    46261 tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc aatttaaaga
    46321 tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag cactaagtcc
    46381 ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa gaggcgtgat
    46441 tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg gtttgtctat
    46501 cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag ttgaaatagc
    46561 taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg ctgcattgaa
    46621 tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc aaggtgtaac
    46681 ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc gaatcaccat
    46741 tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg aagagcatat
    46801 tattcgcggg atggtgggca gagatttagt tgatcgttat cctgaaagaa aaccggatat
    46861 tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg atcacgagcg
    46921 aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag gaatagctgg
    46981 actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt cttatggaag
    47041 caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga ccgttccttc
    47101 agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag gactgaatct
    47161 gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa gtcaaaaagg
    47221 agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa agatgcgtat
    47281 aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt
    47341 cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg agccaacccg
    47401 tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa tggcagcaaa
    47461 tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga tgtgtgatcg
    47521 aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag atgcagatca
    47581 ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa caggcaaaaa
    47641 atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct gatctttttg
    47701 gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa ttgatgacgg
    47761 atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat agccatatat
    47821 tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat ttgtcggtag
    47881 ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat cgccaaatga
    47941 acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag
    48001 gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca ggattgctga
    48061 tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct tttccagctg
    48121 ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc agccttcact
    48181 tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt caatggcgaa
    48241 caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct ttcttggtaa
    48301 aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc tcttatcaag
    48361 ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg tttttgacaa
    48421 ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac
    48481 tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg
    48541 cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct caagcaggta
    48601 catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg acttccggcg
    48661 gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta ttgaacaatg
    48721 gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga ttgatcttgc
    48781 ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag gagaacgccg
    48841 gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc gctaaaagcg
    48901 aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta ccattcgtat
    48961 aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat agggcgctaa
    49021 aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca tcaggaggat
    49081 aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa atgtatcgta
    49141 aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt caaaatgcca
    49201 gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt cttagcggaa
    49261 aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa
    49321 aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa agaggaatat
    49381 tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac tatcgacatc
    49441 gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa atatttgcct
    49501 gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga attggaaaat
    49561 gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc
    49621 aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg tgcgaaagca
    49681 gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat tgcaaaatct
    49741 gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca cgaggaatct
    49801 cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg ttctgcttca
    49861 gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa
    49921 ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc tatttcttat
    49981 gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt gaaaaattcg
    50041 atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg tgaaaaagtt
    50101 gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg ttttgtttgg
    50161 ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata agggactgtg
    50221 ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa
    50281 attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg tgggaaaaag
    50341 tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg agagaaatcg
    50401 aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt
    50461 ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag cttggggtac
    50521 taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg aaattcgctg
    50581 ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg gactggtatg
    50641 agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat ttatacgcta
    50701 gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa
    50761 tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt aacaaaatca
    50821 aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt cttgtgactg
    50881 gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct ggagagggga
    50941 tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct tcaggaacgc
    51001 gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg aaagaatcga
    51061 tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa
    51121 aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc
    51181 caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa gaagtccttc
    51241 ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct agggttttag
    51301 gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg caactagctc
    51361 aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca gtggctaatg
    51421 tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa
    51481 gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat tcaattttag
    51541 gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa ctagcagaaa
    51601 aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa gatataaatg
    51661 gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt tattca
//
LOCUS       DACDKL010000003        50767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDKL010000003 DACDKL010000000
VERSION     DACDKL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.003"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            34..423
                     /locus_tag="IV329_000100"
     CDS             34..423
                     /locus_tag="IV329_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAP8939310.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            599..1342
                     /locus_tag="IV329_000101"
     CDS             599..1342
                     /locus_tag="IV329_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939311.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1335..2915
                     /locus_tag="IV329_000102"
     CDS             1335..2915
                     /locus_tag="IV329_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8939312.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3166..3645)
                     /locus_tag="IV329_000103"
     CDS             complement(3166..3645)
                     /locus_tag="IV329_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8939313.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3661..4413)
                     /locus_tag="IV329_000104"
     CDS             complement(3661..4413)
                     /locus_tag="IV329_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8939314.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4530..4600
                     /locus_tag="IV329_000105"
     tRNA            4530..4600
                     /locus_tag="IV329_000105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4562..4564,aa:Gly,seq:ccc)
     gene            4961..5686
                     /locus_tag="IV329_000106"
     CDS             4961..5686
                     /locus_tag="IV329_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8939315.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5941..6207
                     /locus_tag="IV329_000107"
     CDS             5941..6207
                     /locus_tag="IV329_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8939316.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            6204..6635
                     /gene="ruvX"
                     /locus_tag="IV329_000108"
     CDS             6204..6635
                     /gene="ruvX"
                     /locus_tag="IV329_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8939317.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6660..6965
                     /locus_tag="IV329_000109"
     CDS             6660..6965
                     /locus_tag="IV329_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8939318.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            7534..8370
                     /locus_tag="IV329_000110"
     CDS             7534..8370
                     /locus_tag="IV329_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8939319.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            8435..8869
                     /locus_tag="IV329_000111"
     CDS             8435..8869
                     /locus_tag="IV329_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939320.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(8937..10259)
                     /locus_tag="IV329_000112"
     CDS             complement(8937..10259)
                     /locus_tag="IV329_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8939321.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(10413..12053)
                     /locus_tag="IV329_000113"
     CDS             complement(10413..12053)
                     /locus_tag="IV329_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8939322.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12383..12853
                     /locus_tag="IV329_000114"
     CDS             12383..12853
                     /locus_tag="IV329_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8939323.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            13482..14585
                     /locus_tag="IV329_000115"
     CDS             13482..14585
                     /locus_tag="IV329_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8939324.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            14869..15549
                     /locus_tag="IV329_000116"
     CDS             14869..15549
                     /locus_tag="IV329_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8939325.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            15639..16673
                     /locus_tag="IV329_000117"
     CDS             15639..16673
                     /locus_tag="IV329_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8939326.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            16687..17022
                     /locus_tag="IV329_000118"
     CDS             16687..17022
                     /locus_tag="IV329_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939327.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            17006..19486
                     /locus_tag="IV329_000119"
     CDS             17006..19486
                     /locus_tag="IV329_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939328.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            19542..21101
                     /locus_tag="IV329_000120"
     CDS             19542..21101
                     /locus_tag="IV329_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8939329.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            21098..22462
                     /locus_tag="IV329_000121"
     CDS             21098..22462
                     /locus_tag="IV329_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8939330.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            22633..23703
                     /locus_tag="IV329_000122"
     CDS             22633..23703
                     /locus_tag="IV329_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939331.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            23718..24386
                     /locus_tag="IV329_000123"
     CDS             23718..24386
                     /locus_tag="IV329_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939332.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            24406..24984
                     /locus_tag="IV329_000124"
     CDS             24406..24984
                     /locus_tag="IV329_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8939333.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            25387..26298
                     /locus_tag="IV329_000125"
     CDS             25387..26298
                     /locus_tag="IV329_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8939334.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(26554..27471)
                     /locus_tag="IV329_000126"
     CDS             complement(26554..27471)
                     /locus_tag="IV329_000126"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8939335.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            27673..28110
                     /gene="zapA"
                     /locus_tag="IV329_000127"
     CDS             27673..28110
                     /gene="zapA"
                     /locus_tag="IV329_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8939336.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            28168..28715
                     /locus_tag="IV329_000128"
                     /pseudo
     CDS             28168..28715
                     /locus_tag="IV329_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            28830..31190
                     /locus_tag="IV329_000129"
     CDS             28830..31190
                     /locus_tag="IV329_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8939337.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            31277..31591
                     /gene="trxA"
                     /locus_tag="IV329_000130"
     CDS             31277..31591
                     /gene="trxA"
                     /locus_tag="IV329_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8939338.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            31773..33551
                     /gene="uvrC"
                     /locus_tag="IV329_000131"
     CDS             31773..33551
                     /gene="uvrC"
                     /locus_tag="IV329_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8939339.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            33696..34595
                     /locus_tag="IV329_000132"
     CDS             33696..34595
                     /locus_tag="IV329_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939340.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            34588..35850
                     /locus_tag="IV329_000133"
     CDS             34588..35850
                     /locus_tag="IV329_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939341.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            36013..37911
                     /locus_tag="IV329_000134"
     CDS             36013..37911
                     /locus_tag="IV329_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8939342.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(38210..38929)
                     /locus_tag="IV329_000135"
     CDS             complement(38210..38929)
                     /locus_tag="IV329_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8939343.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            39266..39598
                     /locus_tag="IV329_000136"
     CDS             39266..39598
                     /locus_tag="IV329_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939344.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            39585..41588
                     /locus_tag="IV329_000137"
     CDS             39585..41588
                     /locus_tag="IV329_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8939345.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            41607..42077
                     /locus_tag="IV329_000138"
     CDS             41607..42077
                     /locus_tag="IV329_000138"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8939346.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            42098..42682
                     /locus_tag="IV329_000139"
     CDS             42098..42682
                     /locus_tag="IV329_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8939347.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            42697..43683
                     /locus_tag="IV329_000140"
     CDS             42697..43683
                     /locus_tag="IV329_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8939348.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            43673..43984
                     /locus_tag="IV329_000141"
     CDS             43673..43984
                     /locus_tag="IV329_000141"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8939349.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            44006..45787
                     /locus_tag="IV329_000142"
     CDS             44006..45787
                     /locus_tag="IV329_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8939350.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            45780..47156
                     /locus_tag="IV329_000143"
     CDS             45780..47156
                     /locus_tag="IV329_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8939351.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            47163..47798
                     /locus_tag="IV329_000144"
     CDS             47163..47798
                     /locus_tag="IV329_000144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8939352.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            47828..49183
                     /locus_tag="IV329_000145"
     CDS             47828..49183
                     /locus_tag="IV329_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8939353.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(49375..>50767)
                     /locus_tag="IV329_000146"
     CDS             complement(49375..>50767)
                     /locus_tag="IV329_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8939354.1"
                     /translation="PINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKNT
                     QLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEVT
                     EHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKTQ
                     GVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPID
                     FDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVIE
                     DDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIEQ
                     AIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRVP
                     YYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILASG
                     LSIVKEKIKKYEL"
BASE COUNT        16713 a         8777 c        10624 g        14653 t
ORIGIN      
        1 aaaaaaaata aaaaaagcat ccaaagttac ttattgaaag aaaaagaaaa ctatcctgtt
       61 caacatgcta cgaaaaaaag gtggacaact gtccgttatg gtaatagcga tcactatatc
      121 cattacgatg aaatccttta tctaatgagc atgccaggtt ataaaaataa aataggccta
      181 gtattgaaag atgaagagat tctcgtaaac ggcacaatta aaaaatataa aaaatcactc
      241 gaatatgagt atttatgtaa agatttaaaa gcttatatca ttaatactga aaacattaca
      301 tctattagca gagtaaacga aatagtaaca tttgcaaatg gagaacaact agagatggga
      361 aagagaagta ttgataaact aaagaatttt atgcaagaaa agatagaaaa tgaatcattt
      421 taaagaaaac aaagactcat atcttacctg ttagaagagt gttttcctgt agaagagaac
      481 aacccaagta gcagcctagt cgtttctatc cttttttgtt atgcactctt tttgacattt
      541 actccaccaa taacgaaggg tacaataaaa agtaataaca aacaaaagga gtacagaaat
      601 gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa caagcattaa aagacttgtc
      661 tctctcgata agacaagggg aaatttttgg atttttagga cataatggtg ccggaaaatc
      721 aactacgatt aaaagtttag ttagtattat tgaaccatca tcgggaacga ttttagtaga
      781 tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa aaaatcggtt atgtgcctga
      841 ttccccagat atttttttac aattgacggc tggagaatat tgggacttga tttcagctgc
      901 ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca gaattgacag cgctttttga
      961 tatgtatagt catcaaaatg aaacaatcgc aagtttttca catggaatgc gtcaaaaaac
     1021 gattttgatc ggcacgttgc tgccagatcc agatatttgg gtattggatg aacctttgca
     1081 aggacttgat cctcaagcag catttgattt gaaagaaatg atgaaggctc atgccgcaaa
     1141 aggaaaaaca gtcatttttt ctacacatgt attggatacc gcacaacagt tatgcgataa
     1201 actggctatt ttgaagaaag gtgaattgat ttatcagggg gcagttagtg aattattgaa
     1261 tggctcacct gatgaaacgc tagaaaagat ttatttgaaa atggctggaa ggcaagcgac
     1321 aggagatgga ccgtatgtat aagcgacaat tattagaatt aattcgagtt aatcttttat
     1381 atgttaatcc acagctcacg aataaagcaa gagagaaagg aaaaaaggga aagcgattaa
     1441 ctatctatct tttgtctcaa aatctttttt cagggctgat ttttttactg atttacggat
     1501 ttgctatgtt tgctatgaac tttgttaaat tgccaggttt ctttacttat tacgtggcac
     1561 tttttggtat gttaggttta actcaaagta tatcagtcat ttataatatc ttttttgaag
     1621 gaaatgacct gcaagtattt ttgcctctgc cttttggaca agaacaggtt ttttcagcaa
     1681 agattttggt agttgctttg accatcattc cttttgtttt tccgcttcta gtcatgttta
     1741 ttttaaccgg atggcgagct gatctaggga ttttgcttac tatcattatc tcaatccttt
     1801 tgttttctct atttttgatt attctttttt gtctggctag tttgattgtt tttggtttgg
     1861 ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc tagtatcttg cttggcagct
     1921 cgatgctgat cgcatttgct ggaatcatgt ggatgaatca tcaaacgggg actattgaaa
     1981 atgagttaac agatcggcat ccgatttcga tattattgcc attctatgat gctgccagtc
     2041 agccactttc tcaacaaggt ttattgggtt ggggaatact agctatcacg gctctgggat
     2101 ttttccttgc tgtaaaacag ctgatagtac caaagcttac agagcaactg actgattcaa
     2161 acagtgagtt caaaataaaa agaacccata aaaagaacca aaatgtgcat cagcttttat
     2221 ttagttacaa tgctcaatta gtaaaagaac ctaatttgat tatgcaagtt ctttcttctt
     2281 ccttgttaac tccaatcatt tttattcttg cttttgccct aggaggcgaa ataaaattaa
     2341 ctgatttaga taataagtta atcggtgttg ttttcctggt agggatcgca ttggctttct
     2401 taacagttaa tcagacttct tttatcagca atcttatttc tttggatcaa gaaaattttc
     2461 tgtttatccg ttctttgcca atctctatga accaatattt aaaagaaaag ttcagattcg
     2521 gatggatatt gcagagtttt ttaactggtg ggatcgcatt attaagcgga ctgtcttttc
     2581 agctgccttt gttttttatc cctagcttgc tatttggttc tttattcggc tgctacttat
     2641 tgtcactacg ttatttcgct agagattatc ggttgctgct gctgaactgg accaatatta
     2701 accaattatt taacagaggt tcaggaagtt tgggtttagt cgcaactatg atgggttcat
     2761 tgatcatcag tattattctt atcgtcttat acggaatgtt ggctttgttt tatccatttt
     2821 ggttagtgaa tattcctgtg atcggtttag ttctaatact ttccgctcta tggatcgttt
     2881 attatcaacg aacgttttgg aaaaaatttg aataaagaag aggggccgtg acaaaaatca
     2941 tgtcgcagcc ccttttttac agatagaggg tgttcaaaag tcagtttctc agtattaagt
     3001 caattttatg taaaaatgtg agaagctatt tttacaaaac tttttttaat actcataatt
     3061 acttcttatt gctcctgcgg ttacttgtcg caatgtttgt gagatatttt gtcatcgctc
     3121 aaggctgaac gtctctttca caaccttttc tttgttacac ttcgtttatt taaaaagccg
     3181 tttttttcgt ttcatcatga ttgcgtcacg ctttactttg aaattacgga tgttatggtt
     3241 aaagacttct gataatacaa gacctaaagc gattgctcca gcaatcataa ttgcttggac
     3301 cgcataatga gccgcttctc gataggattc agtaacgaga tttcgaaccg cttgataagc
     3361 taaaccacca gggactagag gaaccatccc gggaatattg aaaatcgtta cgggcatttt
     3421 caaacgttta gagaaaagat cgctgatgaa tgctactcct aaagaaccaa gcaatgaacc
     3481 aaaagcagca gatccgccta tttgaacaca gatccagtag atcatccagc caaaagcacc
     3541 agaaagacca caacaaatca atgaacgcct aggtacattt gtaataacgg caaatgccgc
     3601 agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc cccatacttg tgttcctttc
     3661 ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg atcatcgtag cagtcatcat
     3721 cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca gctagcaaat cacggatagc
     3781 gttcgtaatt tgaactccag gaactaaagg catcacacat ccgataatga ttagatcctg
     3841 attgcttcct aagccaaaac gtacgctgta gatagcggct atgccaatca ataaagaaga
     3901 taaaaattcc gataaaaatt tgattctaaa aaatttcaaa ctatatagat aagctgcgta
     3961 tcccaatcct ccaatgacta aagtcacagg taaatcggtc atctttccgc caaataacac
     4021 cataattgtt ccactgatca ctgccgcgct aacgatttgc aaccagacag gaaagaaaga
     4081 acggtctcgg tcgattgctt gcagttcgca gtaaagttct tctaatgtta gctctgaagc
     4141 aacatattta cgtgaaagat catttacttt tactactttt tctaaattaa tcgaacggtt
     4201 ggtaatttgt tccattcgga tggtcgaggt acgatcaaga ccaataaaga gaccggtttg
     4261 agtgacatag ctgaccaaac ggaaatttcc agaagctaaa gcaattcgac tcatcgtatc
     4321 ttctacccga tacatctccg cattgctttc catcatgatt ttcccagcga gtaaacaagt
     4381 gtccagcaac agatctaatt cttttcccgt catagcatct tctccaataa ttgattgatt
     4441 agttttattt tactacaaaa cataccagaa gcgtaaagaa taaaaaaact tgaggaagga
     4501 ataaaagagg cgtatactgt ttttgtattg cgggtgtagt ttagtggtaa aatcccagct
     4561 tcccaagctg atgtcgcggg ttcgattccc gtcacccgct taaaataggt tgtgaaaaag
     4621 ctgctctgca acaagtaata agaacagcca agcaaaaaca acttcttatg tttttaactt
     4681 ggatggactt attaccgcag atgcaagctt ttgcgcaccg tttaccaagc ggttgtgaaa
     4741 tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa taataaatac cttacagata
     4801 gagtggtgac tgattagaga cgaggaatcc gtctcttttt tgtattttta agccatctta
     4861 aaacaggttt tattttttgc tgtttctatt tctttctagt atttatttat aggggaacat
     4921 atgttaagat ttagggagct gttagtaaag gagtaaagaa atgagcaaaa aaacaaccaa
     4981 ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca ttcggtgttt ccaccacaca
     5041 aaaagattca gatatctttc agcagattac aactattttt gattccacac cacaagcttc
     5101 tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa gaaatagaaa tcaatgggaa
     5161 tcagccggaa tttacaaaag aggatcttag cctttccaaa ggaacatggg aaagctattc
     5221 aaatattgat aaattgaaca gagtaggaca agccaatgct atgcttggaa aagatttgtt
     5281 ccctactgaa aagagagagc ctttgtatat tgacccgaca ggttggaaac aaaagaaatt
     5341 aagtgacggg caatggttat ataatcgcag ccatttaatt ggtttccagc tgactggtca
     5401 aaataacaac attaaaaact tgatgacagg gacacgttcg ttgaatgctc catacatgct
     5461 tgctcacgaa aatgatattg cggcatatat caaagaaaca aatcatcatg tacgctaccg
     5521 tgtcactcct cattttgagg ggaatgaact ggtagccagg ggagtgcaat tggaagcaga
     5581 aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc tacaatgttc aagatggcta
     5641 cacgatcaat tatgagacag gacaagcaac aaaaacaaaa aattagtagc agctattcga
     5701 attttttatg aatattatga aaaaattgtg taaaaacata cattcaaaga aatatagcgt
     5761 atactttatg aaaagagtac ttttgataaa gctacagaaa aaaggaaaaa aacatacaaa
     5821 tagtgtttct taatttattc ggaagtttgg aatatttgtt tgtagttttc cctttcttcg
     5881 agtaaaataa ggttgtagaa ggtaagctgg aattaccttg cgggaaagag gtgtagaatc
     5941 atgggtttta cagatgaaac cgtacgtttt ggttttgacg atagtcgcaa aaaagaagtg
     6001 agcgaaacat tagcgattgt ttatcgggct ttggaagaaa aaggctacaa tccaatcaat
     6061 cagattgtcg gctacttgct ttctggagac ccggcctaca ttcctcgtta tcgtgatgct
     6121 cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg aagtaattgt caaggactac
     6181 ctctcaaatc atggagtgaa tttatgagag taatgggctt ggatgtcggt tctaagacag
     6241 taggtgttgc tatcagtgac ccctttggct ggacagctca aggagtagag atcattcgga
     6301 tcaatgaagc agaagaagag tttggctttg atcggttgaa ggaattagta gcacaatatg
     6361 aagtagaccg gtttgtagtt ggattaccta aaaatatgaa caattccatc ggaccaaggg
     6421 cagaagcttc catgtcatac ggagaaaaga tcaaagaact attctccttg ccagttgatt
     6481 atcaagatga acgattaacc actgttcagg cagaacgtat gctagtagag caggcaaata
     6541 cttcaagagc caaacgaaaa aaagtcatcg ataaattagc agctgtgatg atcttacaaa
     6601 attatttaga cggctcaggt aagcaattat tttaataata aaagaaaaga ggtaatctta
     6661 tgacagaaca taaccattcc cacgaccatg aaggacacga acacatcaca ttggtggatg
     6721 accaaggaaa tgaaacatta tatgaaatcc tactaacagt tgacggacaa gaagaatttg
     6781 gacgtaacta tgttctttta tatcctgctg gtgtttcaga agatgaagat gtagaattac
     6841 aagcttatgc atacatcgaa aacgaagatg gaacagaagg cgagttagaa caaatcgaaa
     6901 cagacgcaga atgggacatg attgaagaag tattcaatac attcatggcg gaagaagaag
     6961 aatagaacaa aagaatgaat taataagata taaaaaaagg gattgtgaca aacgtcttgt
     7021 cacaatccct ttttccaaat aaacgatgtt caatcagctg cccctcggca ttcgctcgaa
     7081 aaacggcgaa atatgaagaa ctattttgcc atttgtttct ctaataatca ggtcaaaacg
     7141 ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca
     7201 caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctga
     7261 acggcaggct cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
     7321 gttcaaccaa acgaaaatta tgaggagcta tttttgtttg gctgttctta ttacttgatg
     7381 caggacagct ttttcacaac ctcttttcgt ttagcactaa atttccttaa aaagtagaaa
     7441 agctttcgaa gcctcgtttg tttcttttac tgtataataa aagaaaagag taacaatcgc
     7501 taaaagatat aaacaagaga aaggaaatac aagatgattg gtctgattta ttttgtggtg
     7561 atcgttctgg cgaacacagt tggcgctatt tccgggatgg gtggaggcgt gttgatcaaa
     7621 ccgattcttg atcttatcgg cgcccattct gttgcaggga tttcttttta ctcaacagtt
     7681 gcggtattca cgatgtccat tgtttcgact gtgcgacaag tatcaagcgg gaaatcactg
     7741 aattggcaga ttgtcggttg ggtatctagt ggagctgttg ttggaggagt agtcggcaac
     7801 attgtattcg aagttttttt acagttattt gaaaacgaga agcatgtgca gctgatccaa
     7861 atttttttga cggtattgac cttggttttt gcgttcttct acaccaagca tcatcagcca
     7921 aaatttcatt tgacgtcatg gacttggtac cttatatgtg ggggggttct gggcttttta
     7981 gctagttttt taggtattgg tggtggacca gtcaatgttt ctttgttgat gctgatgttt
     8041 gcattgccaa taaaagaagc aacactatat tctctatcta cgattttctt ttctcaactg
     8101 gcaaaattgg taacgattgc tttaacaagt agctttatgc gctttgattt atctatgttg
     8161 ttttatgtta tccccgctgc tgttattgga ggtttgtggg gagcaagatt tagcagggtt
     8221 ttgtctccaa aaaaagtgac ctttattttt caagtaattg tcctcgtggt tcttttggtc
     8281 aattgttaca atgcctttgt catcttacaa acattaggag aatctagact aacgcaagtc
     8341 aaaatcactg aaacatattg ttctaaatga aaaaaagagt atactatgat aaaaaaattc
     8401 attttaaaaa atcagaaatt ggctggtgat ttggatgtta cgggaaatgc gtttagaaga
     8461 tgcagaagca ctacgtgaaa tcaatgcgga tcaattaggg tatgatatac cattaaccgt
     8521 tacggtgaga caaatgaaga aactgctgaa ggagccagag aaaaattttt tcttggttta
     8581 tgaagatcct acttctaaaa aagcagctgg ctatgtacaa gcggagtcct atgaatcgat
     8641 ttttagcgat ccaatgttca atattatggc acttgctgtg tctaaatatg ctgaaaaaag
     8701 aggcatcgga aaggctttga tgactggcgt agaagaagaa gcgaaaaaac gaggaataac
     8761 gacaataagg cttaattctg cagaatacag agtagaagca caccaatttt atgaacacat
     8821 cggttatcat tccgataaaa tgcaaaaaag atttttgaaa atcctgtaaa tctttatcaa
     8881 aaaaataccc gatcaccaag ccgatacttg atgatcaggt atttttgttt ttttgactaa
     8941 gaaagtaatt gcatcaagaa accagagatg atcaaaacga tggcaccacc aagtcgattg
     9001 cccatttgag caaaagcaat aagttccatc cgattagctg cagataagac ggctacattt
     9061 cctgttccgc ccatcgagtt gttgcataag ccagcagtaa tagttgattc aattggatat
     9121 aaaccaaaca aacgaccgac aaatcctgaa acaactgaaa tcgcgataac acttgtcaga
     9181 cataagacaa caaattgcca tgtcaaagca gaagctaata cattcagatt cagcaacgca
     9241 ataccgatac cggcaagaac agctgctgtc aagtttttga caatcaagtt attgaacatg
     9301 atagctgcat cttcataata ctctggtaat aggttggcga ttttacagat gacaacgata
     9361 ataatcataa atgcgtaagc atggacttta ggaacaaaat agttaccaat ttgtcctaag
     9421 atgaagaaag tcaatgatac catcaaacca acacctaact gtggaatgga aggtttcaca
     9481 gttcgaggtt ttccatcgcc aagatcgcct ttttccatta atttaccatt gccgttgaat
     9541 tttggcattg attgtccagc acgagcaact aatgctgcac cgatgatagc taagacattt
     9601 gtcattgctg tcgcaggaat caggcgggaa atgatagaag aagcatccgt acctaaaaca
     9661 tttgcataaa tcgttgataa aggaacggct ccggctccaa caccaccagc catcgcaggt
     9721 aatgagatgt ataaaacgga atttgcaaaa ccatttccga taagcatgcc cacgagtcct
     9781 acggcaaaaa aggagacgac cattgaaata agtgcaacgg gaataaaacg aacagaagct
     9841 ttcatcaata gattacggtt cataccaagg atgctaccag taatcaaggc agcaatataa
     9901 aagtcccaaa agcccatatt gctgacaaag ttttctgtgg atttcactac atattcaggt
     9961 aagatcccaa atgtagcaat cgcagcagaa gcgaagatcg caaaaacgga accaccaccc
    10021 aaataacttt tgacgatagg gatttttgtc ccgataaaat gaaataaatt accgaaaata
    10081 accaaaactg cgattggtcc aatcatattc gtcggtaatt tccctaaagc cattgcaatg
    10141 atcaggacaa ggaccatgat caaataaagt ggcaaactta cgccggtgat ttttgtggac
    10201 caaaaacctt cttttttctt ttcctcaact gcagttgcag ttgttgtttt ttgtgccatt
    10261 tttacagatc ctttcttatt ctgtatcata tgaaaaaagc caaagaaaag cgacaaaaga
    10321 gcaggagttg gctggccgct gttttgccgc agtcgctttt ttctttttag acgtcataca
    10381 aagtttctgt atgttctttt actataacga tcttaatctt ctaaagattt atattcagga
    10441 acccatttag cagattcaac tgcttctttg atatccgtga tttcttcacg attcaagttt
    10501 tgtgcgacaa ctgattttgc gacagtttca gcaatcgttt gagaaaattc agtgattttt
    10561 gctactggag gcaagatagc tgctcctggt tttgttacat caacgattcc gcctaatgct
    10621 cgactagctt gagaaatgat ttcagcattt actcgtgtag ctgttgaagc aatcaaacca
    10681 agtcctaaac ctgggtacat caaagcatta tttgcttgac cgatttggta agttacacca
    10741 ttatattcaa catcagcagc agggatacct gttccaacta atgcttttcc gtccgtccat
    10801 tctattaaat cttttgcttt tgcttctgcc aattttgttg gattagacaa aggcatgatg
    10861 atcggacgtg gtgtatgagc agccatttct ttcacgattt cttcagtgaa tgctccaggt
    10921 tgggtagaag taccaatcat gattgtcgga tggatcgctt ttacaactgc ttctaagttt
    10981 gtcaattcat cagcattagt gaaatctgag cgtttgcgag cgaatgggat ttgaccagga
    11041 gtcaatcctt ctgtatcttc aaacaataaa ccttgtttat ctacttgata gaaatgttgg
    11101 cgtgcttctt cttcaggtac accttgacgg atcatctcat ctaaaaggat gtttgcgata
    11161 cccacaccag cagtaccagc accaaatgtc aaaattattt gatctttcaa ttgttcaccg
    11221 gatatattca atccgcctaa tacacctgct aggacaacaa ttcctgtacc ttggatatcg
    11281 tcgttgaaag tagtgatttg atcttcgtat ttttctaaga tcgctgcagc atttcctcgt
    11341 ccaaaatctt cccaatgcaa taacaattct gggaataatt cggtagctga ttgaacgaat
    11401 tgatcgatga attcatagta agtttcgcct tctacacgag catgacgatt gcctaagtat
    11461 aagggattat tcaataattc ttcattatta gtaccagcgt cgatcgatac aggcaaaact
    11521 tgtgctggat taataccagc agcagcagta tagaccatca atttgccgat agcgatgtca
    11581 acaccgttca caccccagtc gcccatacct aagattcctt ctgcatcagt cacaacaatc
    11641 aaacgaatgt ctcgtccatc tgccgcgttc ttcaaacttg ctttgatgtt ttctggttcg
    11701 tcgattgaaa gaaatgcagc atcttgcggt ttcaagaaaa tttcgttgta ttgttcgatt
    11761 gaatccgcaa cgacaggatc ataaacgaca ggcatgaatt caactaaatg ttcgcccatc
    11821 aatttataaa acaatgtccg attcgtattg aatagattca ttaagaagat acgtttttcc
    11881 aaatctgttt gtttgcttaa gtattgacca taagcttgaa cagattgttg ttccagtgtt
    11941 tggacagtac ttggaagcat accagtgatt ccatattttg cccgttcttc ttttgtaaaa
    12001 gctgttcctt tatttagaaa aggattattt aataagttta accctgtaag catgaatgat
    12061 tcctcctaag ttttaataca aacgaagtat agaagtcaga tgatgatata gtcaaaaaga
    12121 gcaggtatga taaattttat ttatatgtaa tatagccaaa agaaatcaaa atagcagtat
    12181 ttgttctttt tatatcgaca atccttgctt gaatactatg cgtatctctt atatgctaga
    12241 gaaagaaaag tagaagaata agggatagat ggaatggata aataggattt ctgaggaaaa
    12301 aagcacatga tagcgttgat aggagtcacc tatgtcaggt gataaaaata cataagtaga
    12361 aaattaagag gaggaagaga aaatgaaaga agttcaattg attagaaaaa gcgaactatc
    12421 agaaggcggt tgcaatgcat gtggcgtagt agaagcgaca agctatacat taaaattaga
    12481 ttccaatcaa gcaataatct ctgaactgac agtaggcggt cttgtggaca gtctggcgct
    12541 tgctgaaggt tttactggag aagatattta tgagatgttt tcagaagtcc gtcagttaaa
    12601 aaaaggagaa aaatgcatag aggttcatca tgagagcccg aatgtgaggt tcaaacgtgg
    12661 tgataatgag atgatcttca agaatcatgt gtctgatcac actgaactat atgaaatcgt
    12721 gaatcaaata ctgtctggga tttttgaatt aggaccttat gagttcaagg aagaaaacgg
    12781 taatccaaaa ttaaatgaag aatggcaaga aacgattgaa acacaaagaa acaatccaca
    12841 cttatttcaa tagtcttcca cagtatttcg taaaaatgtt gatgaaaaag aaacaccgtg
    12901 aatcagaggt cgtgaaaaac ctgccctgca tcaagtaata agaacagcca aacaaaaata
    12961 gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagttt ttgaacaccg
    13021 tttaaggaag agactgtgaa gtcaacgttc tgacctgagt attagagaga caaatggcaa
    13081 aatagttcct catattttgc cattttttga gctaataccg agggtcagct gattgaacac
    13141 cgttttatcg agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg
    13201 aaaatggctt tccacatttt tgcataaatt tgacttaata ccgagaaaat ggcttttgaa
    13261 caccgtttat tcaaaaaaag ggactttgac actacttttg tcaaagtccc atcggttttt
    13321 tatctatttt tattacaatc atttcttcca gagtagtgac atctggaatg gctgaaatgt
    13381 aaacagatta tttgcctgta tcaatgtcga agagtaatat aaaaagtgta aacgctatca
    13441 tattgttcat cattcaaatt ttatagagag aaggcattga gatggaaaaa acatatcaag
    13501 caggtacaaa tgaaggaata gtagatttta tcaatatgga agatttagaa atcgcggctt
    13561 cacaagtgat tccagctggt ggttatggtt acatcagcag tggtgctggc gacttattta
    13621 cttatcaaga aaatgaacgg gcattcaatc atcgtttgat tattcctcat gtattaagag
    13681 atgtagaatt acccgataca actacacatt ttgacgaaga aatgctgact gcaccaatca
    13741 tcatggcacc agttgctgct catggattag ctcatgtgaa ggcggagaaa gcttctgcaa
    13801 aaggtgtagc agattttggg acgatctata cagcaagttc ttacgcttct tgtactttag
    13861 aagagataag agaagcaggc ggggagaaag caccgcaatg gtttcaattt tatatgagca
    13921 aagataatgg aatcaatttg gatattcttg aagtggccaa gcgtaatgga gcaaaagcaa
    13981 tcgtattgac agcggatgca acagttggcg gaaaccgtga aacagaccgc agaaacgggt
    14041 ttaccttccc gctgccaatg ccgatcgttc aggcttatca atcaggagta ggccaaacga
    14101 tggatgctgt atataaatct tccaaacaaa aacttagtcc aaaagatgta gaatttatcg
    14161 ctgctcattc tgatttgcca gtctacgtga aaggtgttca atcagaagaa gatgtctatc
    14221 gatcattaga atctggtgct ggtggaattt gggtatctaa ccatggtggc cgtcaattag
    14281 atggggggcc tgctgcgttt gattcgttgc aatatgtggc agaagcagta gataaacgtg
    14341 taccaatcgt gtttgacagc ggtgtacgac gtggacaaca tgttttcaaa gcaatcgcat
    14401 caggtgccga tcttgttgct attggcagac ccgttattta tggattgtct ttaggaggaa
    14461 gtacaggagt ccatcaagtg tttgatttct ttaaaacaga attggaaatg gtcatgcaat
    14521 tagctggaac acaaacagta gaagatatca aaaaaataaa attgcgtgag aatcgtttta
    14581 tttaagaaat aaatgacgaa aaaaatagga gtgcggcaga tgatgccgta ctcctatttt
    14641 tatagttcaa ggatgtcact aatagaataa tttgaaacga aaaaataaaa ttgaaatatt
    14701 ttttcatact taataaaaaa ggaagaatat tcatgtaaac gattttattg tttttttctc
    14761 ttttatacaa aaagggtcat aatttcaagt aagcggatga attcatgagg attttttgca
    14821 tactgtctct actatactta ggtcactaag aaaaaggagt gatttattat gaaaaagaac
    14881 atgattggga cattattact ttcaacactt cttctaggcg gcttggcaac accagctttt
    14941 gctgagggtc aagcgacttc aaaaggagat attaccttta ctgaaccaac aaataccgtt
    15001 gagcctctta accctactga tccttcaaaa ccagtagagc cagcagatcc ggaaaatcca
    15061 gcaactggcc agacaggttc tttaacattg gatgtagttc ctgaattgcc atttggtaca
    15121 cacgagattg aaagcggaac aaaaacgtat caagtagacg cttcaaaaaa cgatacacct
    15181 tatctccaag tatcagatcg tcgaggtgta ggtgcagatg gccaagcaca agggtggaac
    15241 gtgacagttt cggtttcaga ttttgtaaac ggttcacaag ttttgcaagg cgctgaatta
    15301 gattttggaa catccactgt taaaagtacg tcagataacg aatccactgg accaactagt
    15361 caaactgtaa ctgggttgag taaagcaagt gcagcgacac caatatttac agcagcaaaa
    15421 gatcaaggtc tgggaacatg gctttctgtt tatgatccag caaatatcac actgaaagtg
    15481 ccgaaagcag cagctggaac attcacggct gatttgactt ggaacctcgt ggctggccca
    15541 gtagcataga gtatgaaata aattcaacaa actgcggtga aaatactata ggtgaaggat
    15601 tttttaccgc agttgttgtt gaaaggatgt ggtggacaat gaatcaaagt aaacgactgt
    15661 ttgtttcgtt tttttctatt ttaagtctat ttttcatttt tccgtcaatc agccaagcaa
    15721 aggattcagc aggagacttt ggaatcaaac cggtatttcc agagaaccaa atagataaag
    15781 caatcggtta ttttgattta ttagtggcac cggaacaaaa tcagacacta gaagtaatta
    15841 tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc tgtcaatccg gcggtaacga
    15901 gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac gttagatgag acattgccgt
    15961 ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat caatgtatca gcacatgcag
    16021 aaacaactat acctatagaa gtgaaaatcc cagcaaaaag ctttaaggga cgtgtattag
    16081 cgggtattca tgtttcacct aaagaagaag ctgaaacaga aaatgctaca gaaggagcac
    16141 aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt acaagaaagt caagagacga
    16201 tagaaccgga tctgaaactt ttatcaggag atttggacga ggtcaacgca aagccaactg
    16261 ttcagcttcg tttccaaaat cctcagccta ggattatcag caatttgata tttacgagca
    16321 agatctttta tgaaaatcag ctttatattg aaaatacgtc taatgaattt ttagttgcac
    16381 caaattcgaa ttttcacttg aatcttgatt tagctggtga taaagcaaaa gccggcgatt
    16441 accgcgcaga aatcatagca aaatcaggcg acagtaatga atggcgattt acacaaaact
    16501 ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa ttccgttttt gcagtacaag
    16561 aacaatcttt cccatggctt tatgcaggat tggttgctgc agttctagtg ttatggttga
    16621 ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagg agaaacaaca tgatgaggtg
    16681 atttcgttga aaacatcgat cttattggga gggattattg caagtttgct gttcttcagc
    16741 tggcaagagc cagtccaagc agataccaaa agttcaggta cgattacatt ttatgatcta
    16801 agtccagttc cgcatccaag tggttcagtc aatggcgcat caaaacagca gacacagcta
    16861 ccgatttata aagaatctaa tcaacatggc gtattcccgg agacgaatga agaaaagatc
    16921 cgttttcttt atttgatcat tggtatagaa ttatggcttc ttgcgtggtt gttgttatgg
    16981 agaagaaagg aaaaggagga tgaacatgtt gctacaaaat aaattttcat tttttcgctc
    17041 cttctttttt acttcgcttt ttgttttaag tatatttatc ttttcacctt cggcagaagc
    17101 tgttggagca acgcctcctg ccatttctta tgatccatac acgtatgaaa atatcgtacc
    17161 tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt gtccaactaa ctggggctaa
    17221 ccaattgcct aaattgactg ctacttattt gaacgatggt tcaaatgatc cttggaaagg
    17281 tggcacggtc cgttttcatt gggaaagcca tgtgaaacta acaaatgtac ttgatttggt
    17341 gaatatcact gcacaaggat ttgcatttgt ggcgatcatc aatttgccgg aacatgtatc
    17401 aagcagtgat gtattaaaag caattgactg gaacaacgcc tacttgtata ttgccggtgc
    17461 ccccatcaag gttactagcg gtagtttatt atcagcagga agccatacct tgcgttttgg
    17521 gatggggtcg tgggatacaa atactctatt ggcagatatt attgctatta ttacgcgagg
    17581 cgaattcaac gtggacgata tcccgcttca atttgatgtt acagctaatg tcgcgaagat
    17641 gacagaaaac ggagataaat atgatacgag tccaaacaaa attttgacca agggtaaatt
    17701 acaaccttct atgtctaaaa aggccgtttt ttctgttgat ttttatgatt cgaataatat
    17761 tatccaagat cgtatgttca attcgatttt agctccagct gcaggtagat tttatcctaa
    17821 attaactggc ggaaacttga atccaggagc atatgcattt tctgcaagtg cagagataaa
    17881 tacgtggaat agttacatct caacaagtga ttcaacagga aaatataata catttgctga
    17941 taccacagaa tacgtggatg gaaataacca gaaccttcca ggctctacca atagtcggac
    18001 attgacgatg gggctgactg aaggacgttt tgatcagttg ccatttagtc gctttgatcg
    18061 cgtagtcaac tattttacag gaaaaaacgt tacacaagga gcaatactga ctcatactcc
    18121 agcaagcaca agtggattga atcagacagc caatatcgtg tacagtggga aagatgcgca
    18181 aggcggattg ctttctcctg ttgctttaaa ggtattggag cagtacaagc tagacggaac
    18241 ggttttacca acaaacttaa cagccgatag tcagtgggga aagctccttt cttctgcatc
    18301 gtaccaagtc acggccaatt ggtcagcaac tgaactaaaa aagggttcga tccattacag
    18361 gctttattcg aaaggaaccc aaaaattagc gacaggatgg acagatcaac ttttccaaac
    18421 gatccaatca aataacgggg gtgtaaatac agctacttct acactacctg cattggcttc
    18481 agggcaatat tattttgatt atcgcttggt ggacgatgta cttactaaca tgtatccgtc
    18541 ttttgcgtac aaatggcagt ctgaacaagc tggaatcacc actcttcctc aaataacagt
    18601 ggctaatttt ccatctgtga gtgcagatag tcagctaaaa aaccttagca gacaacctga
    18661 gacaggagat cccctgtctg ctctatcagg agatacgatt caagaaaatg tgacattcac
    18721 gttaacaaaa tctggggatg cgataaccga caaaaaaatc acgatctcac ttcctgaaaa
    18781 tactacttat atcaatggtt ccttaaaatt aaatggtaca actatcgcag ataccggcat
    18841 tcaacaagga gtttctgtcc cagcagatct attgagcaaa ataggagata ccattcatct
    18901 tacttacaac tatcaattaa atactgtaga tacttctgta caatccgtca gcatactgac
    18961 aaaggcagct gtactttcca gcaatattac attagcagat ggtgctaagc ttcctaaccc
    19021 agttgtccaa acatctgcta aaacaattct tgtccccaaa caagaattaa cattagttaa
    19081 tgtaccagat gattttacat ttggtaacga tctccccaaa ccgttgaaga caagttatta
    19141 tgaagcaaaa ggcgattttt cttttgatgt gcgggatact cggcttccaa gcacgtctcc
    19201 ttggcaactg acagggacat tgacttcttt atttaagaat gatcaaggtc aagaattaag
    19261 cggaacaaaa ttatacttca atcattctgg atcaaaacaa ttgattcagc aaggtcaaaa
    19321 cacgttgatt tatgagagtg atggaacagc taaaggagag gttctcgttg actttcccga
    19381 taccgatggt ttactcttag aggtaaatag cagtacgaat gctcaacctg gcgcaacgta
    19441 tcaaggcatg gtcacatggg aactgacagc tgggccaacc agctaagtga ttagaatttg
    19501 tatggatctg tgataactgt cacagatcct ctttttcaag gatgagaagg aaacttctgc
    19561 tgttaaaatc agaaatgctt ggaggagtgg acttggaact tttcagtttg cttgaaaaaa
    19621 atgaacaacg gcaaatcaaa cttttccaac ttttattaga ggaaaaagga gagatttccg
    19681 ttaataacgt acttacccgc ttatcagttg atcgggctac cttgaaagaa gatctgtttt
    19741 atttacaagc taacttggaa aatatcaaac aattggaaat tgttatccaa gaagggaata
    19801 ttttattaat cagacatgga aatatagcag cagtcgatgt ttattatcat tgctacttgc
    19861 gctattctac aaaatatcag atacttcttt atctgttgga aaatggctca tttgatcggc
    19921 agaaacttgt catggaactt aatatcagtt cggctacttt gtctcgcaga gtgaaggaat
    19981 taaatgattc tttaaatgag tttcatctga aaataaaaag cgggcggctg accggtccag
    20041 aaagtcaaat ttgttatttt tatttccaat tattttggtt tggacgtcct tatcttgaaa
    20101 acaaaaaaga atacagtgat ttatcaggaa acactttttt agatctgctg caaaaagagt
    20161 ttagttttac tattacagaa gaaggacgat tgaaatttga aatatggctt tccattatga
    20221 agaagcgaat gcgctgtacg aaaaaatcca aaccagatta tagcaatctt cctgttggat
    20281 tgtttcaaac gacacaattt ttattggacc tccatacatt tttgacccgt tacttctttc
    20341 aatatgcctt tccttggaat caaaatgaaa cgttggtttt ttatcttttc atgatcagta
    20401 actttacatt agatacacat aactcgcatg tccaggagtt tttgataagc gaaaaataca
    20461 aagacgacgt agtagctcaa ttgaatcgaa cgtttaaaca atcgatagaa gaatatttct
    20521 ctttttatgt ttttccagaa gagatttcgc aaaagattgc gttgacagtg atgcaaaatc
    20581 acgctcattt tgcctatttt agaggatgga tgaatatttt tggccaagaa ggattaaaac
    20641 agcggttaac tgaagtagaa gataaacaat tcctggtttt atgtgaaaga ttggtcagac
    20701 aaacatttgc tattgttgat tttactgaag tagacgagga aaatgcccgc tttgaattat
    20761 ttgctcgcta tgcatctatt ttgcggacgc tgatcaagca gttagaatta cgtctgcaaa
    20821 ttgcttatga tcttccttat gaaaggctaa tggcggatat cgtgaaagaa atcatccagg
    20881 aacaattgct gaatacgatc aaatatgatt tagtcgaata cgagaaagaa aagcagtatg
    20941 atatcatttt gactagccaa ttgaaagagt acccgtcaca gaagtccgct aaagtttttg
    21001 tattcactag caacgaatat aattatgatt tcccctattt gaaccaattt ttaaaagaat
    21061 gttatttaga aaaattgaat ttaaggatga ataaaacatg aaaatcgtga tcattggtgc
    21121 ttcccatgca ggcatcacag ctgccctaaa tctaagaaaa cttcaaccga aagcagaagt
    21181 tttactgatc gatgaagatc ataaagatgg actaggctat gtttcaaatg gaatcaacct
    21241 ttatttaaaa ggaaagatac gtagtttagc tgaagttgcc cataacatgc ggactcttca
    21301 agaaagtggg gcaaaactga tcaccgaatg gagagtaact gaacttgatc cagacaaaca
    21361 tcagctcatc ctcgcatcca aagaaggaaa aaacgagacc ataacctatg ataaattaat
    21421 cgtagcaaca ggttcctctc ctgtcacctt gtataaacaa attgaagctg aaaatgtcta
    21481 cacctataaa aatctagtgc aatctaagca agtacttgcc gctttaaaag aagcaaaaga
    21541 agtagtgatc ttcggtgctg gttatattgg attggaatta gcagatgctt tgagaaataa
    21601 aggatacatt attcatctag ttgattatat gccgaatgtt ttatcaagat atttcgataa
    21661 ggatatgatc aacagttttc aaaatcagct gcaaactaag caaatcaatt tttatccaaa
    21721 tgaattcttg atcgattgga aaaaatcaga agagaaagta gtatccgtac aacttctatc
    21781 tcaagcaata aaggcagata tggtcatttt ttctgcccaa actcgtccta atacgacatt
    21841 gttaaaagaa aaagtggctt tgtatgagga tgagactgtg atagtcaatg aatatctgca
    21901 gacaagtgat ccggacatct atgcgattgg agatatcgtc ccagtcagtt ttgacaaaaa
    21961 taaacggcat ctctttttac ctttagttac tcgagctgta catatggcaa gagcagtagc
    22021 gctaactttg agtggtcagc caaccgctta cgacctcaga caaaagataa cagcagcagt
    22081 cattatagac tactttttag gaactgtagg gctgacagaa gatgaagcgc cattcttgga
    22141 acaaaatacc ggttcgtgtt caggagaatt tgatctattt ccacaatacg atgaagaaaa
    22201 caaaacagta aatgcaaagc tgatctacca tccggataca ttagaaataa ttggcggaca
    22261 gctgataagc caagaattct tattatcaga tttgaacctg cttgcggata tcgtgaaaca
    22321 caaaactacg attccacagc tagcagttga ggattttgga tttctgtctg aacacacctc
    22381 tcgttttcac tacctcaatg agttagcttt taaagtacta gctaagagag caaatagcaa
    22441 tagagtagat aaaagactgt gagaaatatc acggtctttt attgtgtggg gatataataa
    22501 atattaatca atcgagaata aaaaattcat ataatgaaat agcggaagaa ttttttgagc
    22561 ataatattcg ccaaaaattt atcaagtgat aaaattgttg tataaaatga ttatttcagg
    22621 aggaggagtt aaatgttttt agcattaaat gaaattatgc attcaaagtt acgttatgcg
    22681 ttagttgcag gggtgatgtt tttgatcgcc tacctggtat tttttttgac tgggttagct
    22741 tatggcttag ctcaagataa tcgaacagca gtcgacaagt gggaggcaga ttcgattgtg
    22801 ctttcgaaag atgcaaattc aaatctaggg atgtccatga ttactaagaa gatagcagaa
    22861 gaagtcgaag gtgggaaagt ggcgtatctt gcccagacac ctggagtcgt gactagtaaa
    22921 gacagtacag aagaaggaaa aatcaatgtt agcttcttcg gtatcgataa aaatcaattc
    22981 atcatgccca atcttgttga aggaaaagct tttgataacg atgatgaggc agtaggcgat
    23041 attagtttga aagaagaata tggtctagcc gtaggagata cagttaagtt atctggtagt
    23101 gacaagacgt ttaaactaac tggctttacg gatcatgcca aattcaatgt ctcaccagtt
    23161 ctctatacaa ccatcaatgc ttaccaagag atccgcttcg aaaaagaaga tacaagtgaa
    23221 aatgcacgga ttaacgcaat agttgtcaga ggtaaaatca gtaatttacc tgaagattta
    23281 gaacagatca aaatttctaa attcatcaat gaattacctg gatataatgc gcaagtattg
    23341 acttttggat ttatgatcgg atttttgatc gttattgcgg ctattgtgat cgggattttc
    23401 atttatgtac tgacgatgca aaagattaat atctttggtg taatgaaagc acaagggatc
    23461 acaggaggat ttattgcgcg ttctgtagtg gcacaaacat tcattttatc atttgttggt
    23521 attcttcttg gtctattagg aacagtggga acatcccttg ttctacctga tgcggttcca
    23581 ttccaaagca attggctgtt ttttggcgtc attagcttgt tgatgctggt agtagctgtg
    23641 ttaggcgcac tattttctgt tcggacaatc gtaaaaatcg atccgttgaa ggcaattgga
    23701 taagaaggag gaagagcatg aaagcaatag aatttaaaga agtagaaaaa agtttccaag
    23761 atggcgacca agtagttaaa gcattgaaag aaacaaccat gagtatcgat aaaggggaat
    23821 ttgttgcaat catcggtcct tctggttcag gtaaaagtac ttttttgact ttggcaggcg
    23881 gattgcagac gcctagcagt ggctctgtat ttatcaatga tcaagaattc agtaatcaaa
    23941 aagaaagtga acgcgttaaa ctacgtttta aagaaattgg ctttattttg caagcttcca
    24001 atctggttcc ttttctgaca gtaaaaaagc agttattatt agtagataag atcaaacatg
    24061 aacatcgtca aaaagaagca aaagaattat ttgagcagct tggcgtggat aagttgatca
    24121 acaagtatcc agaagaacta tctggtggag aacggcaaag ggtcgcaatc gctcgtgcat
    24181 tgtacaatga tccgtccatt attctagcag atgaaccaac tgctagtttg gattcagaaa
    24241 aggcacgaga agtagtcgat atcttggcaa aagaatcaaa agacaaaaat aaagcaacga
    24301 tcatggttac tcacgataca cggctgattg acaaatgcga tagaatattt atgatggaag
    24361 atggtgtatt gaaagaaaaa gaataaccaa aaagcaggtg gaaaaatggt ccatcgatac
    24421 aacaaagata cacagcaaaa aattttgaaa gaagcgactg acttgtttat gtccaaggga
    24481 tatctcggca cttcaacaag agagatcgca caaaaagttg ggatcaccca acccaatctc
    24541 tatcattatt tcggtgataa agaaaaacta tatacggcag ttttagaaaa ccacttaaaa
    24601 gatgttggaa aagctttgag agaaatcgtt caaacaagcg aaacgggatt tcaagtaacc
    24661 ttgacaaaaa tggcgcagtt tttgattgag acacatctgg tcgatttgtt tctgatgctt
    24721 cacgatcttg aaagcaatct ttcaaaggaa acaagagatc atcttttctt tctatggaag
    24781 aaaaattatc gtgaaccttt tgaagaaatc ttttcagcaa accaatctgt gctaagaaat
    24841 gggatttcaa aagaaatcgc agcaaggcat tttttcttag tgttagcacc ttacatcact
    24901 cggtcgaaaa gacctgtgga agaaccgcta aatgtggcgc aattgatcga tttatatttg
    24961 aatggtgtat taggagaagc gtaaagctgt tatataggga gaaaaatagt taggtgaaga
    25021 agcaagatat gacgaagatc gttatatctt gtttttttta tataggtagt ttttcataaa
    25081 tacaaatttg ttattctctc atgattatca gggtatattt ttatttatta tgtcggtgtt
    25141 ccaggaagtg ttttaattta ataacgaaga aattaattgt ttgtagaatg aaggaaacta
    25201 tttattgggt agatgaatca ccacgaatag tcaaaaggag atttcaggat atgccaaaaa
    25261 tgaaaaaaat cattttagca gtaatgttat tttctacggt tatatctttt acagcccctt
    25321 ttttgcgtac ttaagctgat tttaagtttt gacccttagt ctggatctac gaggtgagag
    25381 tcaagaatga aaaaaataat tggagctttt gtatttgcaa tcattatcat tattatctgt
    25441 tttcaaatag atgtttttgg tcaaaaaaag atagttggac aaacggtcag cagtacgaac
    25501 agtacggtta caaagacaaa ggaatcattt gatgatacag aacaagagac attaatcaaa
    25561 atcggaaaaa atgaccgtac actatataca gatgactcgc taactacccc acttgcaact
    25621 ataaacggcg gagagctggc ggagtttctt tctgaaacaa aaaatgctta tcagatcaag
    25681 acaaatgatg gctatactgg atatttagcc ctcgaagatg gaaccaaagt cacaaagaat
    25741 atacaaacaa aaccggaaga gctatctgat gcagtgattg tattagatcc tgggcatggc
    25801 ggaaatgata ctggggcatt aagcaatgat gaacaaacag aagaaaaaga tataacatta
    25861 agtacagcca taaaagtaaa aaaggcgcta gaggatgctg gagcaacggt ttatttgacc
    25921 catactacag atgaattagt acagcttgga gatatatgcg actacagtga agaaaaaaag
    25981 gcagatgtct ttatcagttt acacgcagac tctacggaat atgcgaatga agcaactgga
    26041 atcacgacat attattacta tggacaagag gaaacattag cacagacaat tgctgactcc
    26101 ttcactgatc tgcctctgga ttcaagagga atctccactg ggaactatca agttcttcga
    26161 gaaaatcttc aaccttctat tttaatcgag atgggttata tgaataatga tagtgattta
    26221 aaggaattag taacagacag ttatcagcag cagattgctg caagtttgac acaagcattg
    26281 accacttatt ttcagtaata aataagccca gccaaacaaa aatagctcct catatttttc
    26341 gtttggccgg gcttattatc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
    26401 tgagcctgcc gtttaactcc gcgtcacaag gagcaatttc aaaaaacagc ttctcatgtt
    26461 tgatgactat tgtgacttgt gaccgaggag ttggcatgag aacaccgttt attcggaaca
    26521 agggactgtg acatgacttt tgtcacagtt ccattatttt tttagcagct tcaaagcttt
    26581 ttctgtattg gcaaaatgaa gtttggcata tttttcaagc ataggcatgc cgcctttctc
    26641 caatagtttt gccgctactt ggtcagattt cgttccagct cctgatggaa gggtgattcc
    26701 agcttcagct gatgctttgt gtaactcttc caaaaagctg gcacgactga tgatagaagc
    26761 agcagcaaca gctaaatgat attgttctcc tttagtaacg aagtacagtt tttcttgtaa
    26821 ttgatttttt tctaaacgcg catatttttt aaaatttgct tccggcgtaa actgatcgat
    26881 caaaatcgct tctggttttt ctggctcgat tttttttagc agcaaatgga tcgcttgatt
    26941 gtgcaaagcg actttcatcc gaacggcatt gtattttggt tggatctcat tgtatttttc
    27001 tggagataag acaagcagtt tataggggat ggtttttttt aagatgtcag caagctggat
    27061 gatttggcta tcttttaact cttttgaatc tctaacacca agtttacgta attctgcaag
    27121 ctgatcacgt ttcacataag cagcacatac tgttagcgga ccaaaatagc tgccatttcc
    27181 gacctcatca ctacctaaaa cggataattg actgatattt tttggcaaag aagaggctgt
    27241 ttttgctggc ttggcttgtt tatcctgtgc ctccccccat ttatcagctt ctattgctgc
    27301 attcgtacct tgaaacatca cttttccgga tgtataagcg gtaatggtcg tcccgtcttt
    27361 ttttgcagca aagctagtat aaggaatctg tttatttaac aaataagagc gatagtatgc
    27421 tttcatttga ctaagttggt cgtttgttgc tttgataatg acgttgctca taaaattctc
    27481 ctcgttttta tacataaaac tataccgcaa ctcacgactt ttcgttttct aagagattgc
    27541 gagacatagc atttttttaa agtttatcat ataatagtta agtatgtgaa aaatatttgt
    27601 ggagaacggt gaaagaacca acttcatggt acactaaata aaactttcat caaataggag
    27661 gaaatgattc gtatggcgca cgaaaaaaca cgctacaaag cagtgattgc caaccaaaca
    27721 tatactatta tcggacggga aaccaagcat catatggata tcgtaacgaa gctgatcaat
    27781 gaacagttag cagaattgaa acaattatca ccacaaatgg ataatgaaca agcagcaatc
    27841 ttgatggctg tcaatgcgct ttcggatcaa ttgaagaaac aagaaagaat cttggagctg
    27901 gaagaagaaa cagcagaatt aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt
    27961 gtgaaacgca ttgaagcaat cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa
    28021 gcagatcata ccattaaaaa tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa
    28081 ggacaaatta aacaaaaatc ttctaattaa gatcctttta tcaaaataag aaaaatttaa
    28141 aaaagttaga agagaaagga acgtagcatg ctgagtttgc tgattttatt tttattgcta
    28201 attgcgtttt tttctggagc acgtcgcggg tttgctttgc aaatcatatt cgcgattgga
    28261 tatgtattgt catttattgc tgcccagcat ttttacaaac cattagcaag tcatttggaa
    28321 ttatacattc catacccagc tgtcacccca acatcaaagt tagcattttt tgatcaggta
    28381 tttgcttttc acctagatga agctttttat gcaggaactg cttttctgat tattttgcta
    28441 attggctggc tgcttacccg atttgtgggg gtttttgtcc atggattgac gtatgtgccg
    28501 atcttgagac aggtagactg gattgcagga ggcatcctgt cactaatcat ggcttacgtt
    28561 actatatttt tgattttgca gttacttgcc tttgttccat tagtgtggtc caaaatcaat
    28621 tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt accaacaaga
    28681 tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg aaaatatttc
    28741 gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg gcacagctct
    28801 tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa acattggaat
    28861 ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga aaagaagaac
    28921 tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt caagaaactg
    28981 aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa cttgaaaata
    29041 tccgtcccca tatgaaacgg atcgagatcg gagcagactt gaatggagtg gagcttgcac
    29101 aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat gatctttctg
    29161 acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc acgatccctg
    29221 ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact gatgacgctt
    29281 ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct gttcgtgaac
    29341 agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg atcatcacca
    29401 tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta tttggcggtg
    29461 tagtccacga tcaaagtgct tcaggacaga ctttatttat tgaaccaaaa caagtggtgg
    29521 atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt caacgtattt
    29581 taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac aatgcttatg
    29641 tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa gtaaaagcca
    29701 tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga catccactga
    29761 tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat caagccatcg
    29821 tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta ggtctgttac
    29881 aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa atcggtattt
    29941 ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt ctatcgacct
    30001 tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa aaaagtctgg
    30061 tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct ctagcaatcg
    30121 caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg cattatcctg
    30181 aattgaaagt ttatggatac aatcgggcaa atacgattaa tgccagcatg gagttcgatg
    30241 tggatacact aagtccaacg tatcgtttgc tgattggcgt tcctggacga agcaatgcgt
    30301 ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg aagcagttga
    30361 tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat cgccggaaaa
    30421 tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa gaacttcacg
    30481 atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa atggaaaaag
    30541 caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga aaaagcagaa aaaatcattg
    30601 ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag catcagttga
    30661 tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag aaaaataaag
    30721 tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg ttggttacga
    30781 cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa gtagaaatcg
    30841 gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct caaaaagagc
    30901 cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg ccgaatcaat
    30961 tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa tatttggatt
    31021 ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga acaggcgcac
    31081 ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc tttgagtttg
    31141 caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa agcttactaa
    31201 aggtaagcag atggttcgct atccaagtat agaaatgcta tactaatcat agattttaaa
    31261 aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg aagaaacaga
    31321 taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc gtatgcaagc
    31381 accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa ttgctaaaat
    31441 ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca ttccaacatt
    31501 aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt caaaagaaca
    31561 actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa gctgagggtg
    31621 ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta ttcaaattaa
    31681 tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga catgctataa
    31741 taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa aacaaattag
    31801 ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga acgatcattt
    31861 atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact ggcagccata
    31921 atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc gttacagaat
    31981 cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat cctaaataca
    32041 acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat gaaaaatatc
    32101 cccgtttggt gattactcgt aaagtactaa aagacaaagc actgtatttt ggaccatatc
    32161 ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt cctctgagaa
    32221 aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga caatgtctgt
    32281 gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat gaagtgaaaa
    32341 agtttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa atggaaaaag
    32401 ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc cgtgccattg
    32461 agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga gatgtatttg
    32521 gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt tttcgtaaga caaggaaagc
    32581 taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa gatttcttga
    32641 cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa gtcttgattc
    32701 ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg ctgcaaccaa
    32761 aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa gaatgcctca gttgctttga
    32821 atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca gttgaacgtt
    32881 taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat aactctaata
    32941 tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa ccggcaagaa
    33001 aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat gcatcaatga
    33061 gagaagtcat ctatcgcaga tattcacgag tgattcgcga agggttgcct ttacctgatt
    33121 tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc ttagaaaatc
    33181 aattaggaat cgatattcct attgctggca tggcaaaaaa cgataaacat aaaacaaatg
    33241 agttgctatt cggtatagat ttggctgtag taccgcttga gcgaaattca ccagagtttt
    33301 tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc catcggcagc
    33361 tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta ggaccaaaac
    33421 gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca gccacgacag
    33481 aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt gataaattac
    33541 atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca ttttatgtac
    33601 aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc tggtgtctaa
    33661 ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag gatcttgtta
    33721 aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc gatggaaaaa
    33781 tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt ttgatcttgg
    33841 attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt ggggagaaag
    33901 attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa gtaacgattc
    33961 aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag attgaaccaa
    34021 aaatcgattt ttggatggaa aaatttcagg taaaagggaa aaagacggat aaagtaaaat
    34081 ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc catgaaccta
    34141 aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct gaattgctaa
    34201 aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct agccacaata
    34261 tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga aaaatggttt
    34321 tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta ttcttggaat
    34381 ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg atccttcatg
    34441 aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa atctttactc
    34501 gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact ttggaagaga
    34561 ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac caaagatgtc
    34621 tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc ttttatcatg
    34681 ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt caataaaatc
    34741 ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa aacagatgat
    34801 tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc ggaagaaaaa
    34861 atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat gctttatagc
    34921 gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac tggcttgcaa
    34981 tcgatgcttc gagcaagtca attaggcatt tcaccagaag aagtagctag cttgagccaa
    35041 caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat gacagttgga
    35101 gaagataact cgacagtcca atatgtcgta agttatgtag caacgattgt cttgttcatt
    35161 attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa aggcacgcgg
    35221 atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg aaaactaaca
    35281 ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt tggtatagga
    35341 tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat ctcattggat
    35401 aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg cattttgatt
    35461 ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga tacagcaaaa
    35521 gcaattttgc cagtgactta tctatctcta ggcggatata tgcttggatt gatactagga
    35581 gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt cttatcttca
    35641 tatattatgc cggttcgctt agcaaatgag acagtcggta tgggtggagc attgatttcc
    35701 ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa catgtacaaa
    35761 tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt cattgaaaca atcgatttca
    35821 attatgagaa atgaacgaaa aaaagcataa aatatagaaa aatatagaag gaagctggac
    35881 atttattgtt cccagcttct tttttgtata gacctatttc tattttttta ttatattgga
    35941 tatatatatt cattgaaaat agagcataac aactttataa tgttgagtat accaattttt
    36001 ggaggcgttt taatggaccg ctatttagat ttactttcag agaagttccc tacaacggaa
    36061 gatgtcatca cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag
    36121 ttatttttga gcgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt
    36181 tcaggaaatc ttaaagaaaa gatcaaagac cttttctctg aacaaatgac ggaaacggag
    36241 atcagccaat tcactatttt tacggcttat ccagaatatg tgttgacgac tgattggtac
    36301 aaaaaacaag ataaatccca gctgatccat cgtttgatcg atcttttacg gtttacgaca
    36361 gtcaaataca ctcgatcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt
    36421 gaggaactct tgtatatcga tgatcgtatc aacgggaaaa aagactatgt aaaaaaaatc
    36481 attgaacaac ttgtcttgat gaaggaagaa gaaaaatttt taacaaaact tgcgcagacg
    36541 atccaaaaat ctgtcatcga tcatttgcat atcgtgggtg atatttttga tcgaggaact
    36601 caagcagcca aagtaatgga tcgaatcatg gatttttcct ctgtagatat ccagtggggg
    36661 aatcatgata ttttgtggat tggtgcgtat tgcggctctg aggcatgttt attgacgctt
    36721 ttacggattg ccgcacgcta caactattta tttgaattag agaaagaata tggattaaat
    36781 ttacgtccat tattttcttt tgcccatcaa acctatcaga agaatccagt atttacccct
    36841 aaaaacagga aagatctatc tgaaagagag caagaagaat tagaaaagat ccatcaagca
    36901 ctttctatcc tccaattcaa atcagaacat cagttgttga atcgacgacc tgaatttaaa
    36961 atggacgatc gtaaattgtt ggaaaaaatc aactatgagg aatcaacgat cgttttagaa
    37021 ggtcagctct atgagttgaa agggacttgt tttcaaacga tccagccaga tgatcctaaa
    37081 aaattcttgg aagaggaaca aaaagtgatc gatagtttga tgtattcatt ccagcactcg
    37141 ttaagaatgc aaaaacacat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac
    37201 aatcagcatg tactgttcca tggctgtata cctgtagatg aaaaggggca gctcttagca
    37261 tttgaattag accaatctta tcgcggaaaa gaactgctgg gattttttga aaaacagata
    37321 actgaaagcg caaaagattt gactgcaaat gaagatttgg cgaccgattt gatttggtat
    37381 gcgtggagcg ggccattttc gcctttattc ggcaagagta aaatggcaac ttttgagcgc
    37441 tattttctca cagagcctca tacgcacgtc gaaaaaagca atccctatta tcacttgcga
    37501 gatgaagagt ggttcagtga aaaaattttg gcagaattcg atgcaaaaga agaaggttca
    37561 gcaatcatca atggacacac gccagtaaaa gtaaaaaaag gagagtcacc gatcaaagca
    37621 aacggaaaag catttgttat tgacggaggt ctatccaaag cttatcaaaa aacaaccggg
    37681 attgctggct actcattact atgcaattct tacggtttcc aaatcgtgac acattatccg
    37741 ttcttgccta ttgaacaaca atttgcaaaa aaatctgatc aaacgaatgt aaaaaaagtg
    37801 atcgagcagc agctcccacg caaacttaat cggaatacga caaaaggaat ggaattacag
    37861 caacagatta ctcaactcca aaggctgttg gattatcgaa aggacgattg agaaaaatgc
    37921 tagccaacat tgctcaatag actaaaaagc gactgcaaca aaaggcgtgt cgcagtctcc
    37981 ttcggaatta gctcaaaaaa cggtaaaata ttagaagcta tttttctgtt tatttctcta
    38041 atactcaggt cagggcgctg atttcacgac ctcttcctta aacggtgttc tcctgccaac
    38101 tcctcggtca caagtcacaa tatccaacaa acatgagaag ctgttttttg aaattgctcc
    38161 ttgtgactca gagttgaacg gcaggctcac gacctaatca gaaaaacgat taaaattcat
    38221 tcacaaagaa atcgaataaa cgctgttcag atgaatctac acgatgagaa agttccggat
    38281 gccattggac accaaggatt ttgtgcatat tatctttact ttggactgct tcaatgagtc
    38341 catcgttgga aaaggcgatg gcttttaaca aaggagaaag atctttgact gcctgatgat
    38401 gataggaatt cacaaagtat gaatcaggga gaaggtcact taataaactg tccgaaacga
    38461 ttttgacttc gtgagttgca aactgcgggg cagtgggctg ttgttcgtgt tttacggacc
    38521 attttggata taatgaaaga tcttgataaa gtgttccctc caacacgaca ttcaatagtt
    38581 gaagtcctcg gcagactgca aaaatcggtt ttctttgttt caatgcttcc aagatcaatg
    38641 cctgttcgaa ttggtctcgt tgaatatttg tttccgtcag ttttggatga gggtcttcca
    38701 tgtaaagttg cggactaaca tcctgaccgc ctgcaagaat gagtttgtcg attttctcaa
    38761 ttagttgagg cgctgaatct ggagacatga ctggcagaat aagaggtaat ccttgagcat
    38821 ctaaaactgc atccacaaat ccctgcggtg tatagcttac ttgatttcct tggaaagtat
    38881 ctgtcgctcg aatcagttga ttcccggcaa tgccgatgat tggtttcatt tttattcccc
    38941 ctaaagtaga ctaattgtaa caaggattta aaaaaattta aactatgtaa gacttgcccc
    39001 tatacttatc tgttcttgct gtgttactat ctgtaaagag aattttcgtg tgaaattcat
    39061 gacgatagtt gaaaatattg cttaatttgt tataataaaa agtaagcaga aataaaaagg
    39121 ttgggcgtaa gcttcaagga tctagttctt caaggggtga gaaggacatt ttattgaagg
    39181 ggcctgccta tactaagtta tctttagagg tacatacttt caatgaaaaa agaaagcatg
    39241 tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta gaagctttgg aacaaatccg
    39301 aaaagcagaa gaagaaaatg agaagcgtgc tcgaacccta cggcagcagt tgaagaccta
    39361 tgcagaagaa aaagagcaag aattggagat aatccaaacg aacagtcggc aacagctgaa
    39421 acagttggta gaagaaaaag aacaattcga aaagcaagaa ttagaaaaag aaaaaatgaa
    39481 attgaaagca gaagcagaca aggtgcaagc aactttgaaa gaccaatatc aaaagcatca
    39541 gagtcaagca atcggtgcaa tcattgaaag ggtgaaagaa acttatggcc gtcactaaga
    39601 tggaaaaagt gaccctcatt tcagataaaa gaaatcagga gaggatttta caagcagtac
    39661 aaggaatcca aaatgttgaa atccgggatc tgtatcaaaa tagaacaaac aaccagtggg
    39721 tagaaaaata ttttccagat accacgatta ttgataaaga agcaaaatta agcgctttag
    39781 gcaatcggct gaaagacatc caagaagcta tccaattcat acaacaccac ggcgatagcc
    39841 gtcaaaagaa actccatttg aaacgtcaag aactttcctt acaagacttg gaagcaaatt
    39901 attccgaaga aaatttcatg aataaattag aagaagtcct tgatttaaaa gaaaagtggg
    39961 aacagctaac tgaaaaacgt gatcgtttag aagaagagga agaatggctt cttcattggc
    40021 aacagctgga tattgctcca agtatgtatg caagtaagac gaccattttt gagatgggga
    40081 ctgtgagtgc agcaaatttt gaaagtttca aggaagaact aagtaagctt gatgacgttt
    40141 atatggaaga aatagattat acgccaaaac aagtttatct ttcgtatgtt gctttaaaaa
    40201 aatcagttgc tgctatagaa gaaattgctg ggaaatacgg gttcaataaa gaaacgtatc
    40261 cttatgaaca aaatcctaaa gaaaaattgc aagaaaccaa acagcaatta tccattgttc
    40321 acgagcagca aaaaaagata acgacagcgt taggtcagtg cagcggctat atccaagaat
    40381 ttgaatgggt agaagaagtc acacttgccg ttgcagaacg ggaaaaagta aaagaacggt
    40441 ttatccatgc cacttatctg atcgttttgc aaggttgggt agatgcagaa gaaaaacaga
    40501 atttgatgca tgtgttaaac gaaactgtat cagaagaaga tatatacgtt agtttcgaaa
    40561 caccaaactc agaagagatt cagcaggaag tccctactaa attgaaaaac catccgctta
    40621 tcgaaccttt tgaattgctg actgaaatgt acagtctgcc aaaatatgaa gaagtagatc
    40681 caacgccttg gatgacacca ttttatcttg tcttcttcgg gatgatggtc gcagatgttg
    40741 gttacggatt gctgatgctc attgggacaa tactagctca aaaactattt gttttacccc
    40801 gcgggatgaa acgtttcgta aaattctttg agattctctc ggtaccatcg atcatctggg
    40861 gattgatcta cagttcattt ttcggacagg cattaccaaa agaactattt ggtatccgct
    40921 tgcctttccc aatcctatct acgacggatg acgtcaatac gattttgatt ttatccgtga
    40981 ttttcggtct gatacagatc ttggttggat tattcgtggc agcaaaggaa aatatcagga
    41041 ggaaagatta cttgagtgct gtcagtgatg gttttgcctg gcaagggatc ctgattggga
    41101 tcgttattgc attgattggt gtcatgttga taaaaaatac agcatttgtt tatcttggcg
    41161 cggggctatc gatcattgct gctttatgta tcttaatcgt gccgattatc caatcgccgt
    41221 ctaaagtcaa gggtgctgcc aaaggagcgt ataatcttta cggattgact ggttatatcg
    41281 gtgatttagt cagttataca cgtttgatgg cactaggtat ttcaggcgga agtattggcg
    41341 cggcattcaa tatgctagtt gctttcatgc cgccagccgc tcgattcagt gtagggatcc
    41401 ttttgatcat cgctttacac gcactgaata tgtttttgac actgttaagt gcctatgtcc
    41461 atggtgcacg attgcagtat gtcgaatttt ttggaaaatt ttatacagga ggcggacgtg
    41521 cctttgaccc gctgaaaacg gcggaaaaat acgtcaacat caatcataag aaaaaaaaca
    41581 ataaataatt ggaggatttt tatacaatga tggattactt aattagtcaa aatggtggaa
    41641 tggtatttgc agtattagcg atggcaacag cgacgatttt ttcagggatt ggttcagcta
    41701 aaggtgttgg aatgacaggt gaggcagcag cagcgttaac aacaagtcaa cccgaaaaat
    41761 tcggacaggc tttgattctg caattgcttc caggtacgca agggttgtat ggattcgtta
    41821 tcgccttttt gatttttatc aacttaggaa atgatatgtc agttgtacaa ggattgaact
    41881 ttctaggcgc ttctctgccg attgctttta caggtttgtt ttcaggtatc gcccaaggga
    41941 aagttgcagc agcaggtatc caaattttag cgaaaaaacc tgaacatgct acaaaaggaa
    42001 tcatttttgc tgcgatggtt gaaacatatg cgattttagg tttcgttatt tccttcctat
    42061 tagtattgaa tgcctaagca taaaggagga aaagcagatg aacgccattg aaaaaatcat
    42121 ctctcagatg aatgaagcag cagagcaaga aagagcggcg ttagagcaag aagagcgaat
    42181 gaagatcgat caaaactttg aacaaaaacg tacgcaaatc gaaacagaac atcaaaaaca
    42241 aaaagaaaaa caaatcgaat tacttgaaaa aaaataccgt cagcttagga atcgccaaca
    42301 agtagaagta cgacaagaaa atttgaatgc gaagcaggaa tttttacgac gattgtttgc
    42361 agatgcaata gctgaaatgg agaattggga tgaatctgaa caaatccagt ttatcaaaaa
    42421 ttcattgtat tcattacctt tgacaggcaa agtaacattc attgctggag aaaaatcagc
    42481 agcttatctt tctcagacat tgctggatga atggaacaat gaacttccct ttatgatggt
    42541 tttaagtgat gaaactgttg ctgatcaagc gggttttctg ataaacgatc aaggagttca
    42601 gtataatttt cttttcagca gccttgtaca agatatccaa ggaacgatga gttttgagat
    42661 tgccaatcaa ttattcgaat aaggagggat cagtgtatgg aataccacga actaaatccc
    42721 ttgattcgcg gaagagagct tgaacttttg tccaatgaga catttgaacg gatgattcaa
    42781 acaaattctg tcgaagcact tggtgaaata ttgaaatcca cgatttacag tccatatatt
    42841 tatgacggat atgaatatga cttcgaacag aatcttttaa aggagcagag ccgtctattt
    42901 cgctggttaa aggaatcagc accggagcct gaaatcgttt ggatctatac gatgcgctat
    42961 actttccata atctgaaggt tttgacaaaa gccgagatga caaatcaaaa tctggaccat
    43021 ttgtatatcg acgatggatt ttattcatta gaaaccttga aggatgccat tcatacgcaa
    43081 gtttcaaatg aactccctga aagcattatg gacagtatac gagaagtacg tgattatttt
    43141 gaagagtcta ccattcttca gggaattgac gtgatttatg atcgacagtt tttgacagag
    43201 cagcgacggc ttgccgataa gctaggatat caagaattgc tgacagaagt cattgctttt
    43261 attgacttga ccaatatcac aacgatggct cgaggaatat tgcagaaaag aacatatggg
    43321 ttcatgacga cggtactttc cagctcggga agtatcgaaa aagaagtatt tttgaacttt
    43381 gtagataaag atatggaaga ttacacgaat tttctattag agacagatta tgctgatttt
    43441 ctgcgccctg tcatccgtga gggaaatctt gatctcgtaa agcttgaaca gttgaaagat
    43501 gattatctaa gttcattgta tcaagaagca caaacccagg ctttcggacc actgccacta
    43561 ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc ttcgtttact ggttatcggc
    43621 aaaagaagcc atttcgaacc agaaacaatc agaaagcggg tgagacaagt ctatgacgca
    43681 taagatcggt gtagttggcg acaaagattc tgttttgcca ttcaaattgt ttggctttga
    43741 tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca attgaagaaa tggcaaagaa
    43801 tgagtatggc gtgatttatg tcacagagca atgtgcagca ttgagcccag aaacgatcga
    43861 acgttacaag gaacaaatga tacctgctat cgtattgatt cccaatcatc aaggatcatt
    43921 agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa gcagtaggtc aaaatatctt
    43981 atagtatgaa aaggagatag aaattttgca aattggaaaa atcataaaag tctccggacc
    44041 tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa gacatgtgtc tagtgggaga
    44101 tctaggagtt atcggagaaa ttatcgagat gcgtggcgat gtggcgtcta ttcaagtata
    44161 tgaagagact tctggaattg gtccaggcga accggttcgc tctactggtg aagctttatc
    44221 tgttgaatta ggaccaggga tcatttcaca aatgtttgac ggaatccaac ggccattaga
    44281 tacctttatg gaaatcacac aaagtaattt cttagggcgc ggagttcaat tgcccgcatt
    44341 ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa gccggagaaa cggtgagtgc
    44401 tggagatgtt atcgggatcg tagaagaaac aaaagttata aaacataaaa tcatggtgcc
    44461 aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt tcattcacta ttgatgaaac
    44521 agtatgtgtc attgaaaccg aaaatggtga caaaaatttg acgatgcttc aaaaatggcc
    44581 agtaagacgt agtcggccaa tcaaagaaaa attaaacccg gatgcaccca tgatcactgg
    44641 acagcgagtg attgatacat tcttcccagt gacaaaagga ggagcagcag ctgtacctgg
    44701 tccttttggt gcagggaaaa cagtcgttca gcatcaaatt gcaaaatggg ccgatgtgga
    44761 catggttgtc tatgtgggat gtggcgagcg tggaaatgag atgacggatg ttttaaatga
    44821 attccctgaa ttgattgacc ctaatacagg agaatcgttg atggaacgta cggtattgat
    44881 tgctaataca tcaaacatgc cagtagcagc tcgagaagct tctatctata cgggaatcac
    44941 aattgcggaa tatttccgtg acatgggtta tgacgttgct atcatggcag actccacttc
    45001 acgatgggca gaagcacttc gtgagatgag cggacgtttg gaagaaatgc ctggtgatga
    45061 aggttatcca gcttatcttg gttcacggct tgcggaatac tatgaacgat ctggaagagt
    45121 cattgcatta ggatcagaag gtcgcgaagg aagtataacc gcaatcagtg ctgtatcccc
    45181 gtctggaggg gatatttcgg aaccagtcac acaaaataca ttgcgggtcg taaaagtatt
    45241 ctggggtctt gattccagtt tagcccaaaa aagacatttt ccttcgatcg attggataca
    45301 aagctattct ctttattcaa cagaagtagg gaagtatatg gatcatatcc ttcaagctga
    45361 ctgggcatcc atggtcaccg agggcatgcg tattttgcag gaagaacagc agctgaatga
    45421 aatcgtgcgt ttggtaggga ttgattccct ttcagataat gaccgtctga cattagaagt
    45481 ggcaaaatcc attcgtgaag attatttgca gcaaaatgct tttgatgatg tcgatacatt
    45541 cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc ttgacctttg gtgaagaagg
    45601 acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc atggacggta ccgtatcgat
    45661 tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca gaattagaaa aactagatca
    45721 aattattggt gaaatcaagc aaacgatgca aacgatcgtt tcagaaggag gcatgagcaa
    45781 tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc attgatggcg gttgaaaaag
    45841 ttgctggtgt caaatatgaa gaattaatcg aagtccgtat gcaaaatggc gagattcgtc
    45901 gtggacaagt attggaagtc caagaagata aagcaatggt ccaaatattc gaaggaacaa
    45961 gtggtatcaa tttacgtgac tcttctgtac gatttttagg ccatccttta gaactaggtg
    46021 tatcggaaga catgatcggt cgagtattcg atggtttggg acgaccgaaa gataacggac
    46081 cagagatcct gcctgaaaag catttggata ttaacggaga agtaatcaat ccagtagctc
    46141 gggattatcc agatgagttt atccaaacag gtatctctgc aatcgatcat ttgaatacat
    46201 tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg tcttcctcat aaagaattag
    46261 ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc agatgatttc gccgtagtct
    46321 ttgctgctat tggaatcact tttgaagaag ctgaattttt catggaagat tttcgtcaaa
    46381 cgggcgcaat cgaccgttct gtcatgttta tgaatttagc aaacgaccct gcaatcgaac
    46441 gtatcgctac tcctcgtatg gctttgactg ctgcagaata tcttgcttat gaaaagggaa
    46501 tgcatgtttt ggttatcatg acagacatga ccaactatgc agaggcattg cgagagattt
    46561 ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc tgggtatctt tacacgaact
    46621 tagctacttt attcgaacga gcaggaagaa tccgcggact aaaaggatcg gttactcaaa
    46681 ttccgattct aacaatgcca gaagacgata aaactcatcc aatccctgac ttgactggtt
    46741 atattacaga aggacagatc atcttgacta gagagcttta caagagtggg attcagccac
    46801 caattgatgt actcccgtct ctttcgcggt taaaagataa gggaactgga cccggaaaga
    46861 caagagaaga ccatgcagct acgatgaatc agctttttgc agcctatgca caaggaaaac
    46921 aggcaaaaga attagccgta gttttaggtg aatctgcttt atctgatgta gacaagatct
    46981 atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa tcaaggattc tacacgaacc
    47041 gttcgattac tgaaacactt gatctagggt gggaactgct atcgatgttg ccaagaaccg
    47101 aattaaaacg aatcaaagac gacatgctgg acaaatatct gccagaaggg aagtgagttc
    47161 tgatggcacg attgaatgtg aatccgaccc gcatggaatt gactcgattg aaaaaacagt
    47221 tgacaactgc cacaagaggg cataaattat taaaagataa gcaagatgaa ctgatgcgtc
    47281 aattcatcct gttgattcga aagaacaacc agctgcgtca agaaatggaa gagtctatga
    47341 cagaagcaat gtctgatttt gtattggcaa atgcttcgat cacggaacct ttcatcgaag
    47401 aattatttgt tttgccggcg gaaaacgttg atttgtctat cgtagacaaa aatatcatga
    47461 gtgtaaaagt acctgtgatg aattttgaat atgatgaaca gctgacagac acacctttgg
    47521 aatacgggta tttgaattcc agtgccgaac tggatcggtc aatcgatcgg tttactaccc
    47581 tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac ttgtcaactg atggcgagtg
    47641 aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata tatgacgatc cctcaattag
    47701 aagaaacgat ctattatatt cgtatgaagc tggaagaaaa cgagcgcgca gaagtaacaa
    47761 ggttgatcaa agtaaaaaat atgggtacca ctgactaagt ggtatctttt tttaaaagag
    47821 gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt ttgccggctg ttcagcttct
    47881 cgcattagga ttttttctcc tgattttagt tggcggcggc ctacttaccc taccattttt
    47941 tagtaatagt gggcatggga caaattttat cgatgcgctt ttcactgcaa cttctgctgt
    48001 ctgtgtaacg ggattgacaa cattgaatac agcagagcat tggaacagtg ctggtcaatt
    48061 tttgatcatg cttttgattg agatcggcgg tcttggtttt atgatggttc cgattctatg
    48121 ctttgcattg ataaagaaga aagtcagtct cagcacacgt attatattaa aagaagcgct
    48181 gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt tacatcctaa aattggcctt
    48241 tgttatccaa ttgattggta cggttgcttt atcttttgta tttgtgcctg aatttggctg
    48301 gttgaaaggt atctggtaca gtttgttcca tgcaatctct agtttttgta atgcagggtt
    48361 tgatttgtta ggagacagtt tagccaatca ccagcaaaat gtttatttga tcatggtggt
    48421 atctgctttg atcatttctg gtgggttagg cttcatcgta tggcgtgatt tactggaata
    48481 tcatcagaaa aagaagatga cgattcattc aaaaatcgca ttaaccgtta caggtaccct
    48541 tttattaggc ggattcgttg tgttcttttt cacagagcgc aatggggcaa cgctagtgga
    48601 agggaatatg tttcaacgat tggcaaacac cttttttatg agtgtgacac ctagaacggc
    48661 tggatattat tcagttgatt acctccatat gagccatgca ggtctgattt taacgatgtt
    48721 tttgatgtac atcggcggaa catctgggtc gacggccggc ggattgaaaa caactacgtt
    48781 aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga cgtacgagag cggaagcatt
    48841 tggccgtacc atccgacaag gcgcagtgtt gcgggcactt actttatttt ttgttacgtt
    48901 gacgttgtgt gtagtcgcca tcatgatttt atcggtgact gaaacgatac ctaaaacttc
    48961 tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt ggtacagtag gtcttacgat
    49021 gggattgacg ccagacttga ctgcaatagg gaaattgatc attatagcgc ttatgtacat
    49081 tggacgagtt ggtgtattga ctgtggtctt ctctctgatg acaaaagcac aaagaacaca
    49141 atcaaaatac aaatatccag aagaaagtgt catgattgga taatttcatc ttctgacaga
    49201 aacctagtga tttagtaaaa taagaaaata aaataatttt tcttgcttct tggatacttt
    49261 aagtgcaaaa tgttcgttta ttttttgagg cactacccgt tgttgaacct cgggtagtgc
    49321 ctctttttta ttgaaaggaa tcaataagat ttgattcctt tatttatcaa taggttataa
    49381 ttcatatttt tttatttttt cttttacgat acttagtcca gaagctaaaa tttcaaggtt
    49441 attagaggat agggaaattc gaacgaatgc atctggttgc gcacgtgtag taaagcgtct
    49501 ggaatgataa agtcggatac cgttagcaag aaagtcattc tcaattatct tagggtcaat
    49561 ctcaggatca actggaagaa ttcgatagta agggacacga ttcgttggtt tttctacttt
    49621 gaaaagagaa tcaaaaagct gattggctct ttttgtaaga cttaatttag tttctaagat
    49681 cctatttgct tcatttgaat ggaggatttg agtgatgact tccacatcta atccagaagt
    49741 tttcacattt atattaaaaa ttgcttgttc tatcttgctt tggacatgta ctggaactac
    49801 aagataagca actcgcaatc cagaacaaat aaattttgtc attccagcta agtagattgt
    49861 ttggttaggt aataaatttt ggaaaggagg aatgatcgta tgttgatagt aagttgttaa
    49921 aaatgaatgg atatcatctt caataaccca tagaccttct ttttcaatca cttcggctaa
    49981 ttgctctcgt cttttgtgag agatttgaaa ccctatagga ttattacaag aaggcataag
    50041 aaagacacca tgaatcttct tttttctaca ttcctgaatc aatatatcag gtcgcattcc
    50101 ttgctcgtca aaatcaattg gaacgatctc taaatgatat aactgagcca attcaatgaa
    50161 attagcgtat gtatatcggt ctgttgctat tctatcacct ggagagaaaa gtgcagaaag
    50221 tgtgatagcc aatccatttt gaacaccgga aacaatagat agagattcta aggacggaat
    50281 gactccttga gtttttaacc aatttgctgc agtagtcaat tgaaaatttg tgcctttggg
    50341 atcttggtaa ttaagcaaac caacaagatt tgggttatta ctagttgatt gaataaacgg
    50401 gacaacgaat ttattacatt cttcaaaaga actaaccaga ccaaaatcaa tgatatgttc
    50461 ggttacttca ttggtagaga tggtgatgga ctcaaagaca ttgggagaaa taaatgtgcc
    50521 acttccaata ttcgtataca gcaaaccttt ctcaattcca tatttataag ccttgccaac
    50581 ggtagtaaaa tttaaatcta aataatcagc tagctctctt tgggagggta actgagtgtt
    50641 tttctgtaat ttattcttta aaatatcttg ttctaattgt tgaatcaacg atcgataata
    50701 aggacgcttc aatttttgtt tgtctggtcg ccaagttaac gggtaattgt cataagaatt
    50761 aataggc
//
LOCUS       DACDKL010000004        49234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDKL010000004 DACDKL010000000
VERSION     DACDKL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.004"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..309)
                     /locus_tag="IV329_000147"
     CDS             complement(<1..309)
                     /locus_tag="IV329_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8939355.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     N"
     gene            671..1711
                     /locus_tag="IV329_000148"
     CDS             671..1711
                     /locus_tag="IV329_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8939356.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1809..1997
                     /locus_tag="IV329_000149"
     CDS             1809..1997
                     /locus_tag="IV329_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8939357.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2055..2408)
                     /locus_tag="IV329_000150"
     CDS             complement(2055..2408)
                     /locus_tag="IV329_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8939358.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2939..3361
                     /locus_tag="IV329_000151"
     CDS             2939..3361
                     /locus_tag="IV329_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8939359.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3358..3588
                     /locus_tag="IV329_000152"
     CDS             3358..3588
                     /locus_tag="IV329_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8939360.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4086..4286
                     /locus_tag="IV329_000153"
     CDS             4086..4286
                     /locus_tag="IV329_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8939361.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4313..5506
                     /locus_tag="IV329_000154"
     CDS             4313..5506
                     /locus_tag="IV329_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8939362.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5574..7139)
                     /gene="guaA"
                     /locus_tag="IV329_000155"
     CDS             complement(5574..7139)
                     /gene="guaA"
                     /locus_tag="IV329_000155"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8939363.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7198..8121)
                     /locus_tag="IV329_000156"
     CDS             complement(7198..8121)
                     /locus_tag="IV329_000156"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8939364.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8317..8922
                     /locus_tag="IV329_000157"
     CDS             8317..8922
                     /locus_tag="IV329_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8939365.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9230..10246
                     /locus_tag="IV329_000158"
     CDS             9230..10246
                     /locus_tag="IV329_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8939366.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10387..11688
                     /locus_tag="IV329_000159"
     CDS             10387..11688
                     /locus_tag="IV329_000159"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8939367.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11734..12396
                     /gene="deoC"
                     /locus_tag="IV329_000160"
     CDS             11734..12396
                     /gene="deoC"
                     /locus_tag="IV329_000160"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8939368.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12417..12815
                     /locus_tag="IV329_000161"
     CDS             12417..12815
                     /locus_tag="IV329_000161"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8939369.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12881..13963
                     /locus_tag="IV329_000162"
     CDS             12881..13963
                     /locus_tag="IV329_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8939370.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14175..15737
                     /locus_tag="IV329_000163"
     CDS             14175..15737
                     /locus_tag="IV329_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939371.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15730..16863
                     /locus_tag="IV329_000164"
     CDS             15730..16863
                     /locus_tag="IV329_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939372.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16863..17819
                     /locus_tag="IV329_000165"
     CDS             16863..17819
                     /locus_tag="IV329_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939373.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17918..19084
                     /gene="deoB"
                     /locus_tag="IV329_000166"
     CDS             17918..19084
                     /gene="deoB"
                     /locus_tag="IV329_000166"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8939374.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19108..19926
                     /locus_tag="IV329_000167"
     CDS             19108..19926
                     /locus_tag="IV329_000167"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8939375.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19943..20650
                     /gene="deoD"
                     /locus_tag="IV329_000168"
     CDS             19943..20650
                     /gene="deoD"
                     /locus_tag="IV329_000168"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8939376.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20948..21634)
                     /gene="gpmA"
                     /locus_tag="IV329_000169"
     CDS             complement(20948..21634)
                     /gene="gpmA"
                     /locus_tag="IV329_000169"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8939377.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21806..22387)
                     /locus_tag="IV329_000170"
     CDS             complement(21806..22387)
                     /locus_tag="IV329_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8939378.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(22520..23203)
                     /gene="rpiA"
                     /locus_tag="IV329_000171"
     CDS             complement(22520..23203)
                     /gene="rpiA"
                     /locus_tag="IV329_000171"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8939379.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            23522..23935
                     /gene="rpsL"
                     /locus_tag="IV329_000172"
     CDS             23522..23935
                     /gene="rpsL"
                     /locus_tag="IV329_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8939380.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            24019..24489
                     /gene="rpsG"
                     /locus_tag="IV329_000173"
     CDS             24019..24489
                     /gene="rpsG"
                     /locus_tag="IV329_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8939381.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            24583..26670
                     /gene="fusA"
                     /locus_tag="IV329_000174"
     CDS             24583..26670
                     /gene="fusA"
                     /locus_tag="IV329_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8939382.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26842..28029
                     /gene="tuf"
                     /locus_tag="IV329_000175"
     CDS             26842..28029
                     /gene="tuf"
                     /locus_tag="IV329_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8939383.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            28436..28744
                     /gene="rpsJ"
                     /locus_tag="IV329_000176"
     CDS             28436..28744
                     /gene="rpsJ"
                     /locus_tag="IV329_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8939384.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            28775..29404
                     /gene="rplC"
                     /locus_tag="IV329_000177"
     CDS             28775..29404
                     /gene="rplC"
                     /locus_tag="IV329_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8939385.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            29432..30055
                     /gene="rplD"
                     /locus_tag="IV329_000178"
     CDS             29432..30055
                     /gene="rplD"
                     /locus_tag="IV329_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8939386.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            30055..30345
                     /gene="rplW"
                     /locus_tag="IV329_000179"
     CDS             30055..30345
                     /gene="rplW"
                     /locus_tag="IV329_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8939387.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            30384..31217
                     /gene="rplB"
                     /locus_tag="IV329_000180"
     CDS             30384..31217
                     /gene="rplB"
                     /locus_tag="IV329_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8939388.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            31260..31538
                     /gene="rpsS"
                     /locus_tag="IV329_000181"
     CDS             31260..31538
                     /gene="rpsS"
                     /locus_tag="IV329_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8939389.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            31561..31908
                     /gene="rplV"
                     /locus_tag="IV329_000182"
     CDS             31561..31908
                     /gene="rplV"
                     /locus_tag="IV329_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8939390.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31922..32578
                     /gene="rpsC"
                     /locus_tag="IV329_000183"
     CDS             31922..32578
                     /gene="rpsC"
                     /locus_tag="IV329_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8939391.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            32581..33015
                     /gene="rplP"
                     /locus_tag="IV329_000184"
     CDS             32581..33015
                     /gene="rplP"
                     /locus_tag="IV329_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8939392.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            33005..33193
                     /gene="rpmC"
                     /locus_tag="IV329_000185"
     CDS             33005..33193
                     /gene="rpmC"
                     /locus_tag="IV329_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8939393.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            33218..33484
                     /gene="rpsQ"
                     /locus_tag="IV329_000186"
     CDS             33218..33484
                     /gene="rpsQ"
                     /locus_tag="IV329_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8939394.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            33542..33910
                     /gene="rplN"
                     /locus_tag="IV329_000187"
     CDS             33542..33910
                     /gene="rplN"
                     /locus_tag="IV329_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8939395.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33947..34255
                     /gene="rplX"
                     /locus_tag="IV329_000188"
     CDS             33947..34255
                     /gene="rplX"
                     /locus_tag="IV329_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8939396.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            34283..34822
                     /gene="rplE"
                     /locus_tag="IV329_000189"
     CDS             34283..34822
                     /gene="rplE"
                     /locus_tag="IV329_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8939397.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34841..35026
                     /locus_tag="IV329_000190"
     CDS             34841..35026
                     /locus_tag="IV329_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8939398.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            35062..35460
                     /gene="rpsH"
                     /locus_tag="IV329_000191"
     CDS             35062..35460
                     /gene="rpsH"
                     /locus_tag="IV329_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8939399.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            35492..36028
                     /gene="rplF"
                     /locus_tag="IV329_000192"
     CDS             35492..36028
                     /gene="rplF"
                     /locus_tag="IV329_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8939400.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            36189..36545
                     /gene="rplR"
                     /locus_tag="IV329_000193"
     CDS             36189..36545
                     /gene="rplR"
                     /locus_tag="IV329_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8939401.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            36566..37066
                     /gene="rpsE"
                     /locus_tag="IV329_000194"
     CDS             36566..37066
                     /gene="rpsE"
                     /locus_tag="IV329_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8939402.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            37081..37260
                     /gene="rpmD"
                     /locus_tag="IV329_000195"
     CDS             37081..37260
                     /gene="rpmD"
                     /locus_tag="IV329_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8939403.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            37304..37744
                     /gene="rplO"
                     /locus_tag="IV329_000196"
     CDS             37304..37744
                     /gene="rplO"
                     /locus_tag="IV329_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8939404.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            37744..39039
                     /gene="secY"
                     /locus_tag="IV329_000197"
     CDS             37744..39039
                     /gene="secY"
                     /locus_tag="IV329_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8939405.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            39101..39748
                     /locus_tag="IV329_000198"
     CDS             39101..39748
                     /locus_tag="IV329_000198"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8939406.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39939..40157
                     /gene="infA"
                     /locus_tag="IV329_000199"
     CDS             39939..40157
                     /gene="infA"
                     /locus_tag="IV329_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8939407.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            40190..40306
                     /gene="rpmJ"
                     /locus_tag="IV329_000200"
     CDS             40190..40306
                     /gene="rpmJ"
                     /locus_tag="IV329_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8939408.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            40324..40689
                     /gene="rpsM"
                     /locus_tag="IV329_000201"
     CDS             40324..40689
                     /gene="rpsM"
                     /locus_tag="IV329_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8939409.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            40717..41106
                     /gene="rpsK"
                     /locus_tag="IV329_000202"
     CDS             40717..41106
                     /gene="rpsK"
                     /locus_tag="IV329_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8939410.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            41186..42124
                     /locus_tag="IV329_000203"
     CDS             41186..42124
                     /locus_tag="IV329_000203"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8939411.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            42152..42532
                     /gene="rplQ"
                     /locus_tag="IV329_000204"
     CDS             42152..42532
                     /gene="rplQ"
                     /locus_tag="IV329_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8939412.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            42804..43643
                     /locus_tag="IV329_000205"
     CDS             42804..43643
                     /locus_tag="IV329_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8939413.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            43619..44488
                     /locus_tag="IV329_000206"
     CDS             43619..44488
                     /locus_tag="IV329_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8939414.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            44478..45275
                     /locus_tag="IV329_000207"
     CDS             44478..45275
                     /locus_tag="IV329_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8939415.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            45478..46224
                     /gene="truA"
                     /locus_tag="IV329_000208"
     CDS             45478..46224
                     /gene="truA"
                     /locus_tag="IV329_000208"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8939416.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(46285..46491)
                     /locus_tag="IV329_000209"
     CDS             complement(46285..46491)
                     /locus_tag="IV329_000209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939417.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(46557..49226)
                     /locus_tag="IV329_000210"
     CDS             complement(46557..49226)
                     /locus_tag="IV329_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8939418.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15887 a         9031 c        10450 g        13866 t
ORIGIN      
        1 attataaatc tgttctcctc cgaaaataaa aatttcttct tcgtttttac ataactcaaa
       61 aacatcttct attgaatgaa caatttcaca accattaaag gtaaacccct tatctctcgt
      121 cagaataata tttcttctgt caggtaaggc tcttccgatt gattcaaggt tcttcctacc
      181 taatattatc ggatgtccct ttgtagtatt tttaacatat tcccagtcct tgggaatcct
      241 ccaaggaatg tcattctctt tgccaatcac tctattctta tccatcgcag caatcaaaga
      301 aactttcatt tataacacag cctttttaac taatttacca tctaatttct tatttctatc
      361 aaaaaacctt ttctcattat cctgcccgtt agttgaataa gcaatttttt ctataagtta
      421 tattatcata ataccacaaa atcacaaatt ttcattcaat tatcattttc aattgcattt
      481 tataaatcat tttgtatgta atttggtatg taagttaaaa tgaaaaaagt agtccatttt
      541 gaagtacatt ttgtcatttt gtactccaaa actgaactac ttacttgcat atatacgcct
      601 ttatagtgga gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa
      661 agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc
      721 tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac
      781 aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt
      841 tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga
      901 tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt
      961 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
     1021 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
     1081 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
     1141 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
     1201 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
     1261 tggttggaat gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt
     1321 tagtactcca gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa
     1381 agctggaaca gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata
     1441 tctttcacgg gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact
     1501 gaaaaataat gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg
     1561 caatgattta acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata
     1621 tcaggttacc actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa
     1681 agtaagatat atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt
     1741 ttcttattaa ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg
     1801 agattgttat tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac
     1861 tacgaatacg agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga
     1921 acgaaacttt aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca
     1981 agacgcagag ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat
     2041 ctgaatttta ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct
     2101 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt
     2161 cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt
     2221 aatgatacaa gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt
     2281 gttaaataac gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt
     2341 ttcattcgag cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt
     2401 ttacgcatac taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat
     2461 agaaaaacat gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg
     2521 atgaatccaa tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa
     2581 tttagtcata ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca
     2641 tgagaaagat aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc
     2701 acctgtactg gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat
     2761 caaagcatat gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata
     2821 tcttttagga tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat
     2881 gaagggagat aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat
     2941 gaaaccatct tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt
     3001 tattgaagat gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt
     3061 ctcgttttct gatatagata actaccttgt caactctttt tctactgttg atacgtacgt
     3121 cactgatttc caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt
     3181 aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt
     3241 tatggatatg agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta
     3301 tcgtcatcga ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg
     3361 aaaacagctt atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg
     3421 gaagcaatta atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt
     3481 cgcatgacag acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga
     3541 atggaaacga aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc
     3601 gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa
     3661 agcatattgt gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc
     3721 aaataaaggc aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga
     3781 cctacaaaat tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc
     3841 tgccatgact ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga
     3901 agcgtcacag gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag
     3961 ctttgctaga gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa
     4021 ctatttgata taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag
     4081 taataatgat aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt
     4141 cagcagacat cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc
     4201 aatctcgaaa gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt
     4261 tttcaaatga aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa
     4321 agacccaata aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga
     4381 ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag
     4441 agaagaatat tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa
     4501 aatgacgttt aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa
     4561 gctcagaaca tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa
     4621 aatggctgtt tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa
     4681 gaagtgtaag tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg
     4741 agcaattgtc cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa
     4801 gcaaaagtct aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat
     4861 ttacaaagaa gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac
     4921 tggaatgcga attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg
     4981 agtactaacc atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga
     5041 accaaaaaca aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt
     5101 atccgaatgg aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa
     5161 cggtattcct acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc
     5221 tggtatccat cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag
     5281 tatgggagaa aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac
     5341 tttaggaacg tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa
     5401 tggagccatt tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt
     5461 tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa
     5521 gataagaaga gccagaaacc cttggtataa gaggatttct agctctccta caactattcc
     5581 cactccactg ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct
     5641 acttcgttta cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag
     5701 tcagcggtca ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta
     5761 cgtccgtcac ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag
     5821 atatcacggt caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt
     5881 acgatttcta gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca
     5941 gggaatggct gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg
     6001 acttcgtctt tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc
     6061 agtccaccta cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata
     6121 acgtcagtat acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt
     6181 gttgcttcgt catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct
     6241 gggtcagata cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg
     6301 ttcaagccga attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc
     6361 agaccgtggt caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc
     6421 ccaacgacac tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact
     6481 tgttcacgga ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca
     6541 cagtgacaaa catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt
     6601 acttctgcat ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc
     6661 ggacaatctt tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca
     6721 ccatggctca tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg
     6781 cttgttactt ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg
     6841 ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa
     6901 ttgaagatct caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca
     6961 gaaagaatga ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc
     7021 aataattcgg aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga
     7081 ctaccgtagt ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa
     7141 tttattaccc ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta
     7201 atattttcgc atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc
     7261 ggctcttcct cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca
     7321 tacatctttt gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata
     7381 attctctgga ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt
     7441 ttcgatcagc acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta
     7501 gatttgctga ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg
     7561 ctgctggatc aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg
     7621 tacttttatc tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat
     7681 atcataactt tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt
     7741 tggataaaga aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga
     7801 caaaatcgtc tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat
     7861 cccgataata aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa
     7921 caaagattca aattctttca tataaagata aatcagatga gcaagaggta catagatatc
     7981 tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg
     8041 tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta
     8101 gtagttcatt ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta
     8161 acacaaaata aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct
     8221 ttataataga aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag
     8281 actgttgtca tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa
     8341 cgcccagata cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt
     8401 caatttgtaa cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc
     8461 ctgattgatg ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga
     8521 tgcgggtatg gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg
     8581 gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc
     8641 gaacatgttg atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca
     8701 gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca
     8761 ggcgcatatc ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa
     8821 ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca
     8881 aaagataaag gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg
     8941 agcgacgaaa gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt
     9001 gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt
     9061 cgttgttttt tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg
     9121 gtagataaag gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg
     9181 ggggttttgg tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac
     9241 catcagagat atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa
     9301 taacaaagga agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga
     9361 acatcattat aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat
     9421 cggaatgatc gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga
     9481 gagctatttg aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga
     9541 aaatgagatc aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc
     9601 cacaccaaat attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc
     9661 aatcatttta atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga
     9721 atatgagggc gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg
     9781 catgctgaag gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca
     9841 tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac
     9901 tgtccaaggt ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat
     9961 tttttgcggg aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa
    10021 aaaaatccca gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat
    10081 gattccgcaa ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg
    10141 tttcctgatc gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac
    10201 aaaattaatt attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt
    10261 gcttatgtaa gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt
    10321 actaaagtgg tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat
    10381 caggcaatgc gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa
    10441 gaggaaatcg agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc
    10501 agtgcattat tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta
    10561 acattggcga ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag
    10621 gtagataagc attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta
    10681 gtagctagtg tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt
    10741 ggtacactag acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa
    10801 tttgtacgca ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca
    10861 ccggcggaca aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg
    10921 ctgattgcca gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg
    10981 gatgtgacca ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca
    11041 aagaccatga cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat
    11101 atgtcagaac ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa
    11161 acattacaag gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa
    11221 atggttgttc ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa
    11281 gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga
    11341 gacccagcga tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt
    11401 cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca
    11461 atgatgctag gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg
    11521 aaactgcata aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat
    11581 agtaacgacg aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt
    11641 gaatcggcaa tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg
    11701 gactttaccg ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat
    11761 actattttga aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa
    11821 aaataccatt tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa
    11881 ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca
    11941 cccgagacaa aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat
    12001 atggtcatca atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc
    12061 gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg
    12121 ttattagata gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat
    12181 tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg
    12241 atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa
    12301 gaagaagcaa tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta
    12361 gcaatcgtat ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg
    12421 cagaactcaa acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc
    12481 cttattccca ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag
    12541 gcatcaacat tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt
    12601 tcaaagcagt atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc
    12661 ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg
    12721 atatgccggt tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac
    12781 tgctgcctta tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact
    12841 gttgaaaaat aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt
    12901 tggtttaggt gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg
    12961 ctctactgat tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac
    13021 agacacaggt ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa
    13081 atgggggaaa gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc
    13141 aaatgaatca gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat
    13201 ttttggtatt ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg
    13261 tacgaacttt gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac
    13321 tttcaaagac aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac
    13381 aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg
    13441 attcaaagcc ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa
    13501 tcaatatgct ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta
    13561 tgcacaaaac gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca
    13621 agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg
    13681 cgaccaatca gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac
    13741 atctacactg aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg
    13801 taaattccct ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac
    13861 agaaggacaa ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat
    13921 ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct
    13981 tgttagacac atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc
    14041 aagctgcctt gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa
    14101 agaagaacgc tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag
    14161 gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag
    14221 aatttggaac attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc
    14281 atgcgttgct tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat
    14341 tattagagcc gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc
    14401 cgaccaaagc caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg
    14461 cctttactgt aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg
    14521 accataaaaa agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca
    14581 acccagatgc ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa
    14641 aaacacttta ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc
    14701 ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga
    14761 tcattttgat tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta
    14821 tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag
    14881 ccgatatgat ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc
    14941 aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg
    15001 tgaaaaactt gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg
    15061 gaaacgggca gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac
    15121 atatcaagct aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg
    15181 gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat
    15241 ctgaaaatat cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga
    15301 attattcagt aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa
    15361 cgaatgaact tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg
    15421 ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg
    15481 atgtaggtgc gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag
    15541 cagtgttact gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg
    15601 ttatccatga aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat
    15661 taggtctatt gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg
    15721 ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca
    15781 gttgttttag gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc
    15841 caaggttatt cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg
    15901 ttaaggcaag caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct
    15961 gggtttttca atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg
    16021 gtggcacttg cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta
    16081 ggtgccttag ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga
    16141 acaagtgaag tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt
    16201 gctttacatg aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc
    16261 agtcaaaatg cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta
    16321 aactcaggat tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa
    16381 acgactttag gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca
    16441 ggaatgagta gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg
    16501 cttggcggtg tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg
    16561 ctatctattg gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt
    16621 attcttttat cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca
    16681 ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc
    16741 gggatcaact tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa
    16801 gagatcgttg cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta
    16861 taatgtcgac tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac
    16921 cgctaattct aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag
    16981 ggctagaggg aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga
    17041 gtccatcact tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta
    17101 tcctattttc tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca
    17161 gcgggacagt aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt
    17221 atggtaaagg tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat
    17281 tagcagatat tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt
    17341 gtgctatttt gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc
    17401 gcctccgttc agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg
    17461 ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt
    17521 ttgctcagtc gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct
    17581 ccatggctgc tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt
    17641 tctttggttt tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga
    17701 ttccgccagt attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt
    17761 tcttagggaa agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat
    17821 cttttagcga ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt
    17881 taaaggcaat aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat
    17941 cgtcatggat tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg
    18001 ttctgataca ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca
    18061 gcttggttta ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg
    18121 ctatgcgacg aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga
    18181 aatcatggga ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga
    18241 tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag
    18301 cggaacagct gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt
    18361 atatacttct gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga
    18421 agaattatat cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat
    18481 tggccgaatc attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa
    18541 tcgccatgat tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc
    18601 aggaaaggac gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac
    18661 tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa
    18721 aaacgatttt tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca
    18781 tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga
    18841 attgcttgaa gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga
    18901 tccgacgttt actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa
    18961 aaaaatgaaa gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat
    19021 tgctgagaac tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa
    19081 ataatctttt taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg
    19141 acccagttta tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca
    19201 ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc
    19261 ccgttctttc ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg
    19321 gcaggcaaga aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg
    19381 caaacagtga cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg
    19441 actaatgcat gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc
    19501 gatcatatca attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc
    19561 cctagatttc cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca
    19621 gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca
    19681 acttatgaaa cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg
    19741 atgtcgactg ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc
    19801 tcttgtatta ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta
    19861 gaaacaactc agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta
    19921 ttgtaatagg aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc
    19981 cgataagatt ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt
    20041 ggaagatcca gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa
    20101 aggagaacgt atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc
    20161 ccatgaattg atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc
    20221 attgtcaaaa gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc
    20281 ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct
    20341 attactgaaa gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt
    20401 tctttcggaa gattctttct acaaagatga tttgacagag acgttccaat tagctgaact
    20461 tggcgtttta ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca
    20521 tgtgcagaca ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac
    20581 agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat
    20641 agcagaataa gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca
    20701 aaaatagctc ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga
    20761 acaccgttta ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt
    20821 caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagaa gttggcagga
    20881 gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt gtcacagtcc cttttttata
    20941 gtgtttttta taggtagtat tttttagcaa ctgttaagtc atcatttaat tcataaacaa
    21001 gtggttgacc tgttggaatt tcaagatcca tgatgtcttc gtcagagata ccttcgatat
    21061 gtttagctag ggcgcgtaat gagttaccat gtgcagctac taaaacagtt ttgttgtcta
    21121 gtaatgctgg agcaatttca tcttgccaga atggcaaagc gcgttctaat gtcactttca
    21181 agttttctcc accaggaata tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg
    21241 cagaaccttc gtcagtttct tccattaatg gaggaagagt atcgtaagaa cgacgccaga
    21301 tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt
    21361 taccatagtg acgttcgttt aagcgccatg atttgatttg tggtacccaa agttgatctg
    21421 aatattctaa gatcaagtta catgttttga ttgcacgagt taaaacagaa gtgtaagcaa
    21481 catcaaattc gattccagct tctttgattt tgcgtccgcc ttcttttgct tcttcgattc
    21541 cttctggtgc tagatttaca tcagcccaac cagtaaattg gttcaatgca ttccattcac
    21601 taagaccgtg acgagaaaaa actaattttg gcatgtttag ctctcctttt aagataggtt
    21661 tatatttctg aaattttcag ttctgtttta ttgtacaatt ttttggaaga gatttccatc
    21721 gaaaaattag ataagattct atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc
    21781 ccacgcaact gttcgttttt aggtattaga tgcataattg ctcgatttct ttttcaggta
    21841 aattgatata tttataacat gttccaactg aaacaccgca ttttgaagaa ataaattgga
    21901 tcgtttcttt tttttcataa tagagacgtc ggattttttt gatcgttttt gcatcgattt
    21961 ttggtcttcc gccgattttt ccttttttac gagcttcatc taatccaact aatgtacgtt
    22021 ctttgatcaa tgcgcactcc atgtttgcta ggttttccat taattggaaa tagatttttc
    22081 ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa gtgcagcccg cgatctttga
    22141 ataattgcgt gagttctgtt aattgccgcg tagatttgcc taatcgatga agttggcaaa
    22201 tgactagtgc atcgccttgt tgcatgtcag cgatcactga ctccaaatga acagcagtag
    22261 aatcattttg ttccacttca tatgtctcat ggaaaatttc ttgacaacca aagtcagata
    22321 attgatcaac ctgagttgtt aaatcaacgt catttatggt tgtgcgtgca taaccaataa
    22381 ttttcatggt gattcctttc tagcagtgat tttctaactg gatataacag taaaaatgta
    22441 gcataaagta cgtcaaaaag atagtttttt cgcaaatttt ccgctgtctt tttttattgg
    22501 atgatcaagc agtttttctt tatttacgac ggattgcgtc aagaatctca acgccttttt
    22561 ctgagccgac aactacttta gaaaccatgt ttaggaaaag cccatgttct actaccccaa
    22621 caagttcatc taaataggta gctaagcttt ctggctgttc gatgacttct agatgcagat
    22681 caatgatgta atgaccacca tctgtggtta agacttctcc gttttcgtct gtacgcaaga
    22741 cgggttggaa tcttttttct tcaaaaatat gaagtagctg ttgggagcca taagggatca
    22801 cttcaactgg gagtgggaac ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc
    22861 aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc
    22921 ctttgatccc ttgaaaatca gcactgattt catcagcacc gtctattgtc aaatcgacta
    22981 cggggacttc atcaatactc tttaacggta ttcctaattt ttccgcttgt tcttttgtcg
    23041 catttgaagt agtaactcct gtgatacgta aaccttcttc acgcatacgg cgaccaatct
    23101 cttctaccat ataataggcg gttgaaccgg tcccgagacc tacgatcatc ccgtccttta
    23161 cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt catctttgcg tccacctttt
    23221 tttagtttga gcatcgtgct ttcctcacta ttttagcgtg atttacggta aaaagatacc
    23281 attctttttt attccaataa agctttttca aaaaaaattg catctcttgt tgacatgttt
    23341 tttcattcgt gatattctaa caaaggtgct ttatctacag gtttctgtga agaaacgtgc
    23401 tgactgttta tcaaagcata tttgataagg atgcagacat tgcatttatt ttttatcaca
    23461 aaaaaagaac cacctggatg tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg
    23521 aatgcctaca attaaccaat tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa
    23581 ttcacctgct ttgaacaaag gatataacag ctttaagaaa actcaaacta acgtaaactc
    23641 acctcaaaaa cgtggggtag caactcgtgt gggtacaatg acacctaaaa aaccgaactc
    23701 agctttacgt aaatatgcac gtgttcgttt gtcaaactta attgaagtta ctgcttatat
    23761 cccagggatt ggtcataact tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt
    23821 aaaagactta ccaggggtac gttaccatat cgttcgcggt gctttagata ctgctggtgt
    23881 taacgaccgt aaacaaagcc gttctaaata cggaactaaa aaacctaaag cttaatttta
    23941 attattaaac aaatgaaact tgcttctact atatagaagt aattactatg aacaatattt
    24001 cggaaggagg agttacggat gccacgtaaa ggtcctgttg caaaacgtga tgttttacca
    24061 gatcctattt ataactcaaa attagtaact cgcttgatca accgtgtaat ggttgacgga
    24121 aaacgcggga ttgcagctaa cattatctat aatgcctttg acttgatcaa agaatctaca
    24181 ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa
    24241 gttaaagcac gtcgtgttgg gggttcaaac taccaagtac cagttgaagt tcgccctgaa
    24301 cgccgcgtta ctctaggtct tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac
    24361 acaatggaag aacgcttagc aaaagaaatc atggatgctg caaacaacac aggtgcttct
    24421 gttaaaaaac gtgaagacac tcataaaatg gctgatgcca accgtgcgtt tgctcactat
    24481 cgctggtaag attctctctg tttggtcggc ttttataagt cgacctcaga tagtgtcctt
    24541 atttttagca actagatagt aaagagagga gaaacaagga gaatgaccag agaattttct
    24601 ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg atgctggtaa aacgactgct
    24661 actgaacgta tcttgtacta tactggtaaa atccataaaa ttggtgaaac tcatgaagga
    24721 gcttcacaaa tggactggat ggaacaagaa caagaacgtg gtattacgat cacttctgct
    24781 gctactacag cacaatggaa gggtcaccga atcaacatca tcgacacacc aggacacgta
    24841 gacttcacag ttgaagttga acgttcattg cgtgtacttg atggtgctgt aactgttctt
    24901 gatgcacaat caggtgttga acctcaaact gaaacagttt ggcgtcaagc aacaacttat
    24961 ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga tcggtgctga cttcttatat
    25021 tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc acccaatcca attaccaatt
    25081 ggtgcggaag acgacttcac aggaattatc gaccttgtaa caatgaaggc tgaaatgtat
    25141 acaaacgatc ttggaacaga gatcgaagaa acagaaattc ctgaagaata ccgcgaatta
    25201 gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg aaactgatga agaacttact
    25261 ttgaaatact tagaaggtga agaaatcact gaagctgagt taaaagaagg tatccgccgc
    25321 gcaacagtta acgtagaatt ctacccagta ctatgtggtt cagcatttaa gaacaaaggg
    25381 gttcaattac tattggacgc tgttcttgat tacttgccat caccgttaga tatcccagct
    25441 atcaaaggga ttgaccctaa aactgatgaa gaagtagaac gtcctgcaga cgacagcgct
    25501 cctttctcag ctttagcatt taaagttatg actgacccat tcgtaggtcg tttaactttc
    25561 ttccgtgtgt actctggtgt tttacaatca ggttcatacg tacaaaacgc aacaaaaggc
    25621 aaacgtgaac gtgttggtcg tatcctacag atgcatgcca actctcgttc agaaatctct
    25681 gaagtttacg ctggagatat cgctgctgct gttggtttga aagacacaac tactggggac
    25741 acactatgtg atgaaaagag tctagttatt cttgaatcca tggaattccc agaaccagtt
    25801 atccaagtcg ctattgaacc taaatcaaaa gctgaccaag ataaaatggg tgtggcttta
    25861 caaaaactat ctgaagaaga tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa
    25921 acaatcatcg ctggtatggg tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt
    25981 gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat cttaccgtga aactttccgt
    26041 gctggcactc aagccgaagg taagtttgta cgtcagtctg gtggtaaagg tcaatacggt
    26101 cacgtatgga ttgaatttac accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg
    26161 atcgtcggtg gggttgttcc tcgtgaatac attccagctg ttgaaactgg tttgaaagat
    26221 gctatggaaa atggtgtctt agctggctat ccattagtag atatcaaagc taaactttac
    26281 gatggttcat accatgatgt cgattcaaat gaaactgcct tccgtgttgc tgcttctatg
    26341 gcattacgtg cagcagctaa gaaagctaat ccagtaatcc ttgaaccaat catggctgta
    26401 gaagttgtta tccctgaaga ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt
    26461 ggacgtgttg aaggtatgga agctcgcgct ggcggccaac aagttgttcg tgcgatggtt
    26521 ccacttgctg aaatgtttgg ttatgctaca actcttcgtt ctgctactca aggtcgtggt
    26581 acatttacaa tgacatttga ccactacgaa gatgtaccaa aatcagtaca agaagaaatc
    26641 attaagaaaa acggcggaaa agctgaataa tttcttaaaa ccggatagtc ctgcaaagag
    26701 attatccaaa gagactatat ttttaatata gtttcgtgta aaataattat cgatgatatg
    26761 ggcatgtggt gtaccttgtg tacatcatat acctatcaga caaataaaaa atatattgta
    26821 tttttaggag gacattttaa aatggcaaaa gaaaaatttg accgttctaa accacacgtt
    26881 aacattggta ctatcggaca cgttgaccat ggtaaaacta cattaacagc tgcaatcaca
    26941 actgtactat ctaagaaaaa cggcggccaa gctatggctt acgatcaaat cgatggtgct
    27001 ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc acgttgagta tgaaacagat
    27061 acacgccact acgctcacgt tgactgccca ggacacgcgg attacgttaa aaacatgatc
    27121 actggtgctg ctcaaatgga cggagctatc ttggtagttt ctgctgctga cggcccaatg
    27181 cctcaaactc gtgaacacat cctattgtct cgtcaagttg gtgttcctta catcgttgta
    27241 ttcttgaaca aagtagacat ggttgatgac gaagaattac tagaattagt tgaaatggaa
    27301 gttcgtgacc tattaacaga atacgaattc cctggtgacg atgttcctgt agttgctgga
    27361 tcagctttga aagctctaga aggcgacgct tcatacgaag aaaaaattct tgaattaatg
    27421 gctgcagttg acgaatacat cccaactcca gaacgtgaca acgacaaacc attcatgatg
    27481 ccagttgaag acgtgttctc aattactgga cgtggtactg ttgctacagg tcgtgttgaa
    27541 cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg gtattgctga agaaacttca
    27601 aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt tagactacgc tgaagctgga
    27661 gacaacattg gtgctttact acgtggtgtt gcacgtgaag acatccaacg tggacaagtt
    27721 ttagctaaac caggtacaat cacacctcat acaaaattct ctgcagaagt atacgtgttg
    27781 acaaaagaag aaggtggacg tcatactcca ttcttcacta actaccgtcc acaattctac
    27841 ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag aaggaactga aatggttatg
    27901 cctggcgaca acgtaacaat ggaagttgaa ttaatccacc caatcgctat cgaaaacggt
    27961 actaaattct caatccgtga aggcggacgt acagttggtg ccggtgtcgt tacagaaatc
    28021 aaagcttaat tcacttaagt tttaaagtaa agcaacattt attcggacgt aatcgctcgg
    28081 aacgaaggtc ttaggatctt tgttccgagc ttttttattt attgcaggat caggaaaaaa
    28141 gacaggagta ttataaatag aagaaacacg aaataaaaga gtcagtattt tctaaatgaa
    28201 actttatgaa aacaaaatgt tttttacaag aaaaagcctt gctttgtgag tttatatata
    28261 ttataatgac aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc
    28321 gtcggctaag aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac
    28381 ggaaattttc gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc
    28441 aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc
    28501 ggataaaatc gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt
    28561 accaactgaa cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg
    28621 cgaacaattc gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa
    28681 aactgttgat gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt
    28741 ataaaactaa aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa
    28801 agtgggaatg acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga
    28861 agctactcca aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat
    28921 tcaagttggt taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt
    28981 tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg
    29041 agaatatgaa gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga
    29101 tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag
    29161 ccgcggacca atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt
    29221 tgcacctaac cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt
    29281 aactatccaa aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa
    29341 aggtaatgtt cctggagcta aaaaatcttt aattactatc aaatcagctg tgaaagctaa
    29401 ataattgtag gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga
    29461 tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga
    29521 aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc
    29581 ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac
    29641 aggtcgtgca cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt
    29701 tggaccaaca ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat
    29761 gaaatctgta ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa
    29821 cttcgacgca ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc
    29881 taaagtattg gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt
    29941 accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac
    30001 aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac
    30061 ttactagacg taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac
    30121 aagaaataca cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta
    30181 gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc
    30241 aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg
    30301 actgaagatt caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac
    30361 tcattaaatt aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc
    30421 gtcgtaacat gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat
    30481 tgttacaacc attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc
    30541 atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata
    30601 acgttgttgc aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt
    30661 tagtacacta tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg
    30721 gtatgaaact tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag
    30781 aaaacattcc agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac
    30841 aattgatccg ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat
    30901 tgatccgctt gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg
    30961 gttctgtagg taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt
    31021 ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg
    31081 gtggtggtga aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac
    31141 cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc
    31201 gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca
    31261 tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag
    31321 ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt
    31381 tcccaagttt tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt
    31441 acatccaaga agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc
    31501 gtggacatgc tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa
    31561 atggcagaac aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga
    31621 aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc
    31681 ttgaaattca caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt
    31741 gcgaatgcag aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt
    31801 gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca
    31861 atcaacaaac gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc
    31921 agtgggtcaa aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc
    31981 taaatggtat gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa
    32041 atttatttca actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac
    32101 aggtcgcgta aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg
    32161 ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat
    32221 caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc
    32281 acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc
    32341 aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga
    32401 tatcgctcgt tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga
    32461 cattgattac gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg
    32521 gatctatcgt ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc
    32581 atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa
    32641 gctaaaggcg gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat
    32701 tggatcacaa accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt
    32761 ggtgggaaag tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg
    32821 gtacgtatgg gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt
    32881 aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta
    32941 gcttctcaca aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa
    33001 tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa
    33061 acaattaaaa gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa
    33121 cactgcacgt attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga
    33181 acaagctaag taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg
    33241 caaagtttat caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt
    33301 cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa
    33361 agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg
    33421 tccattatca gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat
    33481 ctaataacac gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa
    33541 cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt
    33601 gacgatcaaa gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt
    33661 tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc
    33721 cgttatcgtt cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga
    33781 tgaaaatgct gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg
    33841 gccagttgca cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga
    33901 agttttataa tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa
    33961 aggcgataaa gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc
    34021 agcgttcccg aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca
    34081 aaaaccttct caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt
    34141 ttctaacgtg atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt
    34201 agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt
    34261 taaggaagga gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac
    34321 tccatcattg atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa
    34381 aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc
    34441 agttgaagaa ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc
    34501 aatcgctgga ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg
    34561 agaaagaatg tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga
    34621 cttccatggt gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa
    34681 agaacaattg atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga
    34741 catcgttatt gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt
    34801 aggcatgcca ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat
    34861 tgctaaaaac aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg
    34921 cggacgtcca cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact
    34981 tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca
    35041 taagaaggag gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt
    35101 atccgtaacg caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa
    35161 cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa
    35221 gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt
    35281 atcacaaact taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa
    35341 gtaccaaaag tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc
    35401 actgataaag aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa
    35461 tcaagtagaa tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt
    35521 tatccctgaa ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa
    35581 aggtgaattg actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt
    35641 aactttcact cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc
    35701 gaacttcaac aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat
    35761 cggggttggg taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc
    35821 acatccagta gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca
    35881 agtaattgtt aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg
    35941 aactcgtcct ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg
    36001 ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc
    36061 aggaaaaagg catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca
    36121 gcttctattt ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg
    36181 tgacaattgt gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg
    36241 tacgtaacaa tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca
    36301 aaaacatcta cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta
    36361 ccttggataa agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac
    36421 tagttgctga acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat
    36481 acctttacca tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat
    36541 tttagaagaa ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga
    36601 agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg
    36661 ttttgctgct ttagttgttg tcggtgacaa aaacggacac gtagggtttg gtacaggtaa
    36721 agctcaagaa gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt
    36781 tgaagtacct atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg
    36841 tagaatccta atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg
    36901 tgccgtatta gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac
    36961 accaatcaac gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga
    37021 agttgcagaa cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata
    37081 atggctgaat taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc
    37141 gatacagtta aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat
    37201 gaagcaatta aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa
    37261 tttagaaaaa tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt
    37321 aaaacctgct gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg
    37381 taacggaaaa actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt
    37441 gcgtttagga tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt
    37501 cacaaacgtg aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga
    37561 agacggagct gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa
    37621 agctggaatc aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc
    37681 aaaattctct aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat
    37741 ctaatgttta gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta
    37801 tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta
    37861 aatgctgctt cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta
    37921 agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact
    37981 gcttctatcg tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg
    38041 aaacaagggg aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta
    38101 ttggcattta tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt
    38161 ttcgtgaata atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt
    38221 acgatgtttg ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta
    38281 tcaatgatca tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat
    38341 gaagattact ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc
    38401 attttactag tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa
    38461 cgcaagatcc cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg
    38521 ccgttgaaag taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct
    38581 acgccaaatg ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa
    38641 gtgatgatgg agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg
    38701 atcgttgcct tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa
    38761 aacttgcaaa agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag
    38821 tatgtctcaa gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg
    38881 gcattgcttc caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc
    38941 ggtacaagct tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc
    39001 ttaatgctga aacgtaaata tgttggattc attgagtaag aaagcgtgtt gaggtttccc
    39061 ctcaacacac gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg
    39121 gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc
    39181 gcacatctca acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct
    39241 ggaagcaaag tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat
    39301 cgtgaaagag cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc
    39361 acgtacgcta gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat
    39421 tgatgctgtc atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg
    39481 atttatctgc agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga
    39541 agacacatgt gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga
    39601 gacggttaaa aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta
    39661 taaagaacaa ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc
    39721 agatgtacaa aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca
    39781 gttactcttg tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat
    39841 cctctagatg atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc
    39901 gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg
    39961 aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa
    40021 acggacacca agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct
    40081 tacctggtga caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta
    40141 cttatcgctt taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag
    40201 accatcagta aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat
    40261 ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata
    40321 aatatggctc gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt
    40381 acttatatct atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta
    40441 tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa
    40501 gtagataaat taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt
    40561 ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga
    40621 caaaacacga aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag
    40681 aaaaaataat tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt
    40741 aaacgccgtg tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat
    40801 aacactatca tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt
    40861 tcattaggtt tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa
    40921 actgctacaa aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga
    40981 cctggttctg gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact
    41041 gcaattcgtg acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt
    41101 gtttaatgag tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt
    41161 cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat
    41221 tgatgaagaa aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac
    41281 tactttaggg aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac
    41341 taatattcaa atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga
    41401 cgtcactcaa atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga
    41461 aaaaaccctt gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt
    41521 tgatagtgat gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc
    41581 tacgttccat gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa
    41641 taaaaaagaa gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg
    41701 tcgtgtgaac tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt
    41761 gacgatggag atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc
    41821 gaaaatcatg actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc
    41881 tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga
    41941 agagttagac ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt
    42001 acaagaactt acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa
    42061 atcattagaa gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga
    42121 ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc
    42181 tagccaacgt aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat
    42241 tgtgactact gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt
    42301 aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt
    42361 tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt
    42421 caacgatatt gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac
    42481 agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac
    42541 taataacatc actttataga tgattctttt agagtgttat gatgttggaa gatatttttc
    42601 cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat
    42661 aaagtgtttt tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa
    42721 aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac
    42781 tttaggatga agaaggagta atcatggagc caattattga attagaaaag ataaattata
    42841 aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag
    42901 gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga
    42961 tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg
    43021 aagaaaatat ttggacgatc cgacagatgg ttggaatgat ttttcaaaat ccagacaatc
    43081 agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc
    43141 ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt
    43201 ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg
    43261 gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc
    43321 cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga
    43381 ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga
    43441 tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg
    43501 aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag
    43561 aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat
    43621 ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag
    43681 agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca
    43741 taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag
    43801 cggtacagtc catattggag aacgagatat tcagccggat acggataata agaatttaaa
    43861 gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga
    43921 aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc
    43981 actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg
    44041 ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc
    44101 catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag
    44161 aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt
    44221 agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa
    44281 gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa
    44341 agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg
    44401 gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta
    44461 tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt
    44521 ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa
    44581 tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt
    44641 ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct
    44701 ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt
    44761 ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct
    44821 gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg
    44881 aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc
    44941 acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa
    45001 cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag
    45061 ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc
    45121 gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa
    45181 cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat
    45241 tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc
    45301 ttttcttgct gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg
    45361 gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa
    45421 attttataga aaaatcatta ttccagataa ccttttagga acaaaggaga gaaaaacatg
    45481 gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag
    45541 ccaaatgggc gtactgttca agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga
    45601 aaagaaatca cggtctttgg ttcgggaagg acagatgccg gagtacatgc gatcgggcaa
    45661 gtaatccatt ttgattatcc agaggagcgt ccgttagaac gaatgcgttt cgcgctagat
    45721 acccagtcac cagaggatat tgctgtaaga caagtagaaa ttgtttcaga agacttccac
    45781 gcaagatatt tggtcaaaga aaagacgtat caatttcgag tggatatagg gaaaccacgc
    45841 agtccttttc gacgccatta tgcaagttat tttccttacc cacttgattt ggaaaaaatt
    45901 cagcgggcgc ttcctgattt attagggact cacgatttca cctctttttg tgcatcagga
    45961 agctcgatag aggataaaat acgtacgatc tacgaggcta agatggaggt aaatgaaaca
    46021 aaagacgaat tgttgtttac atttagggga aatggcttct tgtataagat gatacggatc
    46081 ttagtaggaa cgcttttgaa aattggcaat ggccgattgc ctgaagatag tatcccagat
    46141 attattgcga aaaaagatcg taatgcagca ggacctacag cacaccctga aggactttat
    46201 ttgtatgaag tgaaatatga ataagaggtt atgatagaag tggtcagctt caagaaataa
    46261 gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga
    46321 agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc
    46381 acaaagagca atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg
    46441 aggcgttgat aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg
    46501 gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac aggttccatc gtttctttat
    46561 tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg
    46621 ccataaaagg ggcgattgcg gtcaaggtac cagacataaa gaataaacaa tgcaatacat
    46681 aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca agtaactgaa agtgacagta
    46741 tgtttacctg ctggaacttt tatcatcaag aatgcgtctt tgaaattgct gaccttgact
    46801 ggtttcccat caattttagc tttccatcct ttgtcataag gaatagttgt caccaacatg
    46861 gaatcttcag cggtatttac agtcccagaa gcttttctgc cagtggcttc catttcgaca
    46921 cctttttctt tgatctgatt cattgcggct tgataggcta caacatctag accaacgact
    46981 ttaggctcca tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt
    47041 gtctcttgag gataatagcc aagatcatag tattgaccag aaatgttgat ttgcgttttt
    47101 cgcgatgtac cattgacagt gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt
    47161 gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat ggacttccca agtgagctct
    47221 tttggctggt tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc
    47281 gtattcaact gattcttcaa agtaggtgta tagaatgtaa aatactgttc atttagacca
    47341 gacaatgtat tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca
    47401 tcattaacgg acaacggtgc agtaaatgct agtggcaatg cattgacatt ctgatacagt
    47461 gtatagtcgc cgctttgacc agcttggcgg aagccatatt tactgaacgt gctttcgttt
    47521 ttagaaatgt tatatttgat tcctgtaaac ccatccatca gcaaagtatt attgttataa
    47581 cggatattca aatttgtccc tcttgaacgg aaacctaatt tatccaggta agaggaagaa
    47641 ttacgatttc ttatagaaga aaacaaactg ataccactgt aaccatagtt gatcgaatcg
    47701 tttgcagata ctggatccag attttccaag cggtaaaacg agtcggaatc tttttttgtt
    47761 tcatttacaa gagctttcaa gtcaggataa ggatcagtat ataaactgcg ggaagcataa
    47821 ttccaatcat caagaatgcc atggatcata ctgttcgtat tgaccagtgc ttcagcagac
    47881 atgatcaata ataacaatat actcagatga ttgaatgaaa gttttttatg ctgataaaat
    47941 cccacagcaa aagcataaat ccctaagaaa accactgtca atacaaagga agtcatcgtc
    48001 acataggtgt agcttccttc aggtttgatg ccccatgcca ccgcaaagag agcagctacg
    48061 accaaaatga ttcctaacaa cagcccacca tcgtcttgtt ctatcttttc ccatcctttt
    48121 cctgcaagca ggagcactaa gaaagagaac agataggcat aacggaataa aaacatattc
    48181 ggtgcatgca taccatgcca gaacaaattc aatggaacaa gataaaaact tgcgataagt
    48241 atggcgaaaa gactgccgta caaaaattta tttttcaacg gtactttccg actggcaaaa
    48301 taaaacagac agaggactaa tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat
    48361 tttgtcgtgt cataaacacc aatcatattt ttcatgatta gatccagata agaagtcgct
    48421 tctgttttca acgtagtgat ctgactcagt gcctcgccat ttgaccgaag atccagcact
    48481 gctggcaata caatgatcat tgaagcgccg cctgccagta aagaagtgat accataagga
    48541 acaatcgttt ttttatattc tgaccagttt gtcaatagac gggcgataaa atataagaaa
    48601 gaaaatacgc caatcataaa acccatataa aaactggata aaaacaagag taaataactg
    48661 ataaataaaa gtttcggccg tttaagatcc ataacacgat gaatccccaa aatcactaat
    48721 ggaagataaa caaatgcatc cagccacatg atgatctccg aatgtgcagt gataaatgag
    48781 atcaatgcgt aactgacgct aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt
    48841 gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag aacatacagt
    48901 gcatctggca tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga
    48961 tagtaagaaa ttaaagataa ataattcaat cctagagaag cattccatgt ataaaaaata
    49021 ctttgctttc caagaagaac atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag
    49081 gcatcacttg ccaaaatact tctgctgctt ccaggataga ttccgatact tagataaaca
    49141 agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta
    49201 aagaatgact ttaaattttt tttcatattt attt
//
LOCUS       DACDKL010000005        48985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDKL010000005 DACDKL010000000
VERSION     DACDKL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.005"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(120..2255)
                     /locus_tag="IV329_000211"
     CDS             complement(120..2255)
                     /locus_tag="IV329_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8939419.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2338..3993)
                     /locus_tag="IV329_000212"
     CDS             complement(2338..3993)
                     /locus_tag="IV329_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8939420.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(3995..4309)
                     /locus_tag="IV329_000213"
     CDS             complement(3995..4309)
                     /locus_tag="IV329_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8939421.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4479..4739
                     /locus_tag="IV329_000214"
     CDS             4479..4739
                     /locus_tag="IV329_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8939422.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            4753..5067
                     /locus_tag="IV329_000215"
     CDS             4753..5067
                     /locus_tag="IV329_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8939423.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5797..6351)
                     /locus_tag="IV329_000216"
     CDS             complement(5797..6351)
                     /locus_tag="IV329_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8939424.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6477..8405)
                     /locus_tag="IV329_000217"
     CDS             complement(6477..8405)
                     /locus_tag="IV329_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8939425.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8711..9427)
                     /locus_tag="IV329_000218"
     CDS             complement(8711..9427)
                     /locus_tag="IV329_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8939426.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9428..11251)
                     /gene="glpO"
                     /locus_tag="IV329_000219"
     CDS             complement(9428..11251)
                     /gene="glpO"
                     /locus_tag="IV329_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8939427.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11265..12761)
                     /gene="glpK"
                     /locus_tag="IV329_000220"
     CDS             complement(11265..12761)
                     /gene="glpK"
                     /locus_tag="IV329_000220"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8939428.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(12897..14357)
                     /locus_tag="IV329_000221"
     CDS             complement(12897..14357)
                     /locus_tag="IV329_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8939429.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14347..15642)
                     /locus_tag="IV329_000222"
     CDS             complement(14347..15642)
                     /locus_tag="IV329_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8939430.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(15974..16651)
                     /locus_tag="IV329_000223"
     CDS             complement(15974..16651)
                     /locus_tag="IV329_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8939431.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16681..17772)
                     /locus_tag="IV329_000224"
     CDS             complement(16681..17772)
                     /locus_tag="IV329_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8939432.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(17873..18307)
                     /locus_tag="IV329_000225"
     CDS             complement(17873..18307)
                     /locus_tag="IV329_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8939433.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(18891..20348)
                     /gene="ascB"
                     /locus_tag="IV329_000226"
     CDS             complement(18891..20348)
                     /gene="ascB"
                     /locus_tag="IV329_000226"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8939434.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20345..22240)
                     /locus_tag="IV329_000227"
     CDS             complement(20345..22240)
                     /locus_tag="IV329_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8939435.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22298..24073)
                     /locus_tag="IV329_000228"
     CDS             complement(22298..24073)
                     /locus_tag="IV329_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8939436.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24123..25619)
                     /locus_tag="IV329_000229"
     CDS             complement(24123..25619)
                     /locus_tag="IV329_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8939437.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25813..27435)
                     /locus_tag="IV329_000230"
     CDS             complement(25813..27435)
                     /locus_tag="IV329_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8939438.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(27884..29197)
                     /locus_tag="IV329_000231"
     CDS             complement(27884..29197)
                     /locus_tag="IV329_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8939439.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29442..29666)
                     /locus_tag="IV329_000232"
     CDS             complement(29442..29666)
                     /locus_tag="IV329_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939440.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29686..30054)
                     /locus_tag="IV329_000233"
     CDS             complement(29686..30054)
                     /locus_tag="IV329_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8939441.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(30252..30962)
                     /locus_tag="IV329_000234"
     CDS             complement(30252..30962)
                     /locus_tag="IV329_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939442.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(30980..31477)
                     /locus_tag="IV329_000235"
     CDS             complement(30980..31477)
                     /locus_tag="IV329_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939443.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31514..31966)
                     /locus_tag="IV329_000236"
     CDS             complement(31514..31966)
                     /locus_tag="IV329_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8939444.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32101..32706)
                     /locus_tag="IV329_000237"
     CDS             complement(32101..32706)
                     /locus_tag="IV329_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8939445.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33156..33776)
                     /locus_tag="IV329_000238"
     CDS             complement(33156..33776)
                     /locus_tag="IV329_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8939446.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33778..34647)
                     /locus_tag="IV329_000239"
     CDS             complement(33778..34647)
                     /locus_tag="IV329_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8939447.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34670..35077)
                     /locus_tag="IV329_000240"
     CDS             complement(34670..35077)
                     /locus_tag="IV329_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8939448.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35096..36385)
                     /locus_tag="IV329_000241"
     CDS             complement(35096..36385)
                     /locus_tag="IV329_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8939449.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36412..36714)
                     /locus_tag="IV329_000242"
     CDS             complement(36412..36714)
                     /locus_tag="IV329_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8939450.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36739..38832)
                     /locus_tag="IV329_000243"
     CDS             complement(36739..38832)
                     /locus_tag="IV329_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8939451.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39321..40085)
                     /locus_tag="IV329_000244"
     CDS             complement(39321..40085)
                     /locus_tag="IV329_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8939452.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40196..41065)
                     /gene="mdcB"
                     /locus_tag="IV329_000245"
     CDS             complement(40196..41065)
                     /gene="mdcB"
                     /locus_tag="IV329_000245"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8939453.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(41090..42166)
                     /locus_tag="IV329_000246"
     CDS             complement(41090..42166)
                     /locus_tag="IV329_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8939454.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(42190..42783)
                     /locus_tag="IV329_000247"
     CDS             complement(42190..42783)
                     /locus_tag="IV329_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8939455.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(42787..44463)
                     /gene="mdcD"
                     /locus_tag="IV329_000248"
     CDS             complement(42787..44463)
                     /gene="mdcD"
                     /locus_tag="IV329_000248"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8939456.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(44453..44752)
                     /locus_tag="IV329_000249"
     CDS             complement(44453..44752)
                     /locus_tag="IV329_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8939457.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(44854..46500)
                     /gene="mdcA"
                     /locus_tag="IV329_000250"
     CDS             complement(44854..46500)
                     /gene="mdcA"
                     /locus_tag="IV329_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8939458.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(46507..47418)
                     /locus_tag="IV329_000251"
     CDS             complement(46507..47418)
                     /locus_tag="IV329_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8939459.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            47538..48395
                     /locus_tag="IV329_000252"
     CDS             47538..48395
                     /locus_tag="IV329_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8939460.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(48582..48944)
                     /locus_tag="IV329_000253"
     CDS             complement(48582..48944)
                     /locus_tag="IV329_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939461.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT        13862 a        10493 c         8286 g        16344 t
ORIGIN      
        1 cttttcacat aaagtgttcc taaatttttc ctaagaaaaa gaacaaaaca tttctcatca
       61 gcaaatgaaa aatattttgt tctttttatc agttgttctt tttatttttt atctcttaat
      121 tatgttgctc tttccaatac gacaatgctt ttcattcctc tagatatcgc cgtgtacatt
      181 tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg gatataagat tacatgatca
      241 aattccagtc cttttgctag actcaaagga atgatttggc agttttctaa tccatatgaa
      301 ttttttttaa tcgattcttg tagcgtttgg gtttcctctt ctgtttgggt gataactgcc
      361 gctttttctc cttttagaga aaccaaaatc tcagctagta ttttcaacaa ttcttcagag
      421 gatcgaaaag agcggtattc gggttctttt ccttgcggtc ggatcgatac gatgtcgatc
      481 ttctcttgat ccacagcata ggttttgaaa agctctgtga ttgctccgct agaacggtaa
      541 ctatttctaa gatcataccg atggatcggc agatggtagt cgtcgaaaca acgcataata
      601 tcagcaaaag tagtggaaga attaaagatt gcctgattct cgtcacctac taaagtaaag
      661 tgagtttttg gaaataattc gatcaataga cctaattgtg ccagggtata atcttgtact
      721 tcatcaatca atagatagcg aaatgctggt acatttacct tttcaacaag taaatgacga
      781 atagctaata taatgactgc ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag
      841 ccatatcgtt tttcataaat tctttcaaac aactgatgct catccaccca tgccatttct
      901 tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa gtttccttgc ataagctaca
      961 atactttgtt ccgaatcatc agaaatcaat ttaccgaata attccaactg cctatcttct
     1021 gtcaacgaga gtacctgatt ctgcacagca ggactttttg cttgtttcaa aagatgattc
     1081 ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga
     1141 gcaggaactt tctcatatat tttttcgatc gtttttttag aaaggactgt ttttccattt
     1201 ttagtaagtc cacggataaa attcggatga tctaaaaaca aaggatctga ttgttttaga
     1261 ttatcgataa actttttgct acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg
     1321 atacgtttga aataagcctc ttcaccttct atttcctctg ctgaaagctg tgaaaccagc
     1381 tgtatgatgg ttagattcag tggatttctt tcacctaatg atggtaggac attcgaaata
     1441 tagtcgataa accgatgatt aggtgataga atcagcaagt catcggatgt catcgtatct
     1501 cgatataaat agagcagata cgcaatacgc tgcatgatag cagaggtttt accacttcct
     1561 gctacgccat ttaccaagat attttgactt ttcacatccc gaatgattgt gttttgttcc
     1621 gattggatcg tagcagtgat agccttcatt tcattggtct catcttggcc taagacttct
     1681 aataatacgt catcttgaat agctacagac gtatcaaaaa acttgaccaa acgatctctt
     1741 tccaaaagaa attgtcgtct ttctttaata accgtatcga ttttattttg acgaaccgta
     1801 taagaagatg gaccaatcat gttattgtaa aaaagttcgg caattggtga acgccaatca
     1861 taaacaagat tttcatctgc atcatctgtg aaatttgctg taccgatata aaaggattct
     1921 tttggctcat tatccagaaa gtctacaacg acttttccaa agtagggact tttcaataaa
     1981 cgttcgatct ttgctagctg attttctgcg tttttctgtt tgatattcat ctgatcgatt
     2041 tctctatttt tcatttcaat catcgaaaaa gtatccaggt tatctaagta actgtcaaaa
     2101 ttcaatcgaa gctcgccagc catttctttc aacgactggt ttccttctat ttttgccgta
     2161 tccatcgatt cccgcagttc gtttttttta tcaattagct tttgatacac gttcttcaga
     2221 tatgcttctt ctaactgttt ttgtgtgttg accatacaca ttctcctctt tttttaaagt
     2281 ggtggaaccg attagtttac ctcttttaag agtcgaatgc aagcttttta acgagtatca
     2341 tttgttagga tatatcagtt cttcaggtcg gacagtttga tataaagaaa atgcaccatc
     2401 aaggtttttt acgtggtagc cagactgctt caagattctt tccgctagat agctacgtaa
     2461 tccgctatga cagctgacga tatattcttg ttggctgtct aattcattta atcggtcccg
     2521 aagttcattc aaagggatat gtttggcttc cttgaatttg cccttttcca attcttctgc
     2581 tgttcgcaca tctaataaaa tttttccttt tgctagttct gtcggcaatt catgccattg
     2641 tacggtttca ctaagccctt ctgcaatgtt cattgctgca tatcctagca tgtttactgg
     2701 atctttggct gagccaaatg gcggtgcata cgtcaattct aattccggta aatcgaagat
     2761 cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc cgtttatcca caccttttgc
     2821 cccaacacct tgtgcaccat aaatctcgcc agtctctgga tggaagatca acttcagtaa
     2881 aatatctgtt gcttctggat aatagcttgc atgatctttt cctgatacat gaatgacttc
     2941 aaaagacagt tcgttttctt tggctgttct ttcacttaaa ccggtggatg cggcagtcat
     3001 tccaaacgtt cgtataattg ctgttccaat accgccttga tttcttcgag aaattccgga
     3061 aatcgtgtcc gcaacttgtc taccttgacg attggctggt gaagcaagag aaataagtgc
     3121 atcttttcca gtgatttgct gttttacaac gatagcatcc cccaccgcat aaatatcagg
     3181 aatattcgtt tgataacgtt cgtctactag gatacccccg cgtatgccta attcgattcc
     3241 agcggtcact gctagctggt tatcaggacg aacaccaatg gataaaataa ctaaatcagc
     3301 agggatggta cgcccatcat ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac
     3361 agcatgagaa gtaatcactt ctactccttg atgagacaat tctttttcta taaaagctgc
     3421 catttcttca tccagtgttg gaagaatatg tggtgcttgt tcgatcacca tgacttctaa
     3481 acctcttctt tttaggtttt ctgccatttc caatccgatg aatcctgcac caacgatcac
     3541 ggcacgcttt ggctgttttt ctaatgcatg catcacttta tcgatatctg gtacatttct
     3601 aatagaaaaa acattgtcgg cttctgttat ccctggaatc gatggaacaa ctggttttgc
     3661 accaggagat aaaaccaatg catcgtactg ttcaatgtgt ttttgaccct catgtacgac
     3721 ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca tgatttggac gcacatctaa
     3781 acaaaaacga gcggataatg attctggcgt ttggacaaga agattttccc gttcactgat
     3841 ttctcccgaa agataataag gcaagccgca atttgcaaaa gaaacatacg gtcctttttc
     3901 aaaaacaacg atttctgcat cttccattaa tcggcgaagc cgtgtagcag cagacattcc
     3961 acctgctaca cctccgacaa taacgatttt catcttattt ccctcctact gttcgatcaa
     4021 accattgatt catgccacca cgtacattca ctacatcata accagatttc tttaattctt
     4081 tagctgcttg tttactcctc atccctgatt gacaaatcac atagacggtc ttttctttgt
     4141 ctccatcata tctgtttata ctttgtaatg gaacattttt agctcctttt atatgcccgc
     4201 cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg attttccaaa agcttttcct
     4261 ttaattctgg gactgttatc gattttattg attgaaatag caaattcatt ttctgcacct
     4321 catttacatt tttataaata cctcatgggg tatattaaac aggagcttta ttaatatgtc
     4381 aaccagaatg actcttcatt tttttcacta ctttttatat ttgcataaaa taccgataag
     4441 gggtattatc taattataaa aatagaggag aatgtgttat ggagattgat aataaaaata
     4501 tcctgaaccg tttgaaacgt acagagggac aaattcgtgg tatccaaaaa atgatcgaag
     4561 acgaaaaaga atgtatggat gtcatcactc aattaagcgc tgttcgttct agtattgatc
     4621 gcgtaatggg catgatcgtt gcagataatc tgaaaaattg ctttgagaaa cctgatagta
     4681 atccagaaga acaagcggct aaactagaac aagcgatcaa gatgattatt aaaaagtaaa
     4741 ggaatgattt caatgaaagt cgttttccat attgatgaat tagaaaaatg gtcagaaaca
     4801 gggaaaaacg taaaaaacct gataaaagct tctccagaaa cgacgattgt cgtatctgta
     4861 aatggcatcg cgattaccgg ttatctagac tctgccaatg cagaattctt agatttgcaa
     4921 ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta atcatatttc agaaagttct
     4981 ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg atttagttga actgcaatcc
     5041 caaggttatg cttatatcaa gccttagtca gtagaggttg cgacagaagt gatcagcttc
     5101 aagaaatttc ggcttatttt tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg
     5161 tgaaaacgct gtcctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
     5221 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag
     5281 gttgtgaaat cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca
     5341 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaaggaag
     5401 agattgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggatttc
     5461 tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttaagg
     5521 aagaggttgt aaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagct
     5581 gctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac
     5641 caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc aaaaacaact
     5701 tctcctgttt ttggtttggt tggcttatta tcgagaagta gctactggga gtctatttat
     5761 caaaaaaagg aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg
     5821 gtactcgaca gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat
     5881 gactctcacc cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata
     5941 gatgacagca gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga
     6001 tagcccgatt aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa
     6061 cagccaaagc cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt
     6121 atctgcaaac ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat
     6181 gaccccgtct agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa
     6241 tgactgtctc agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa
     6301 aagattcgga atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc
     6361 ctacctcctc agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat
     6421 ttcactgttt ttatttttct ttacagccac tgatattatg agctaatttg agataatcac
     6481 ttgctgtttt catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt
     6541 actttatgag ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa
     6601 tcgataaaca gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc
     6661 agacgaacac gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta
     6721 tcttcaccta tatcttcata aactaacgct tctttttggt attcattgac aaatatccga
     6781 tcaccttttg caaagagctg gcgttctttt ttattctcct tcctatttgt aactgctgtc
     6841 ttaggaagaa attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct
     6901 tgatggatca atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct
     6961 cctgtttctc caacccgcaa ctgatattta ggacgaagtg cttcccgatc gaaagccata
     7021 gcagccggca caaaatcttc atgatctccg gcgaatttct tgatttctcc ataatgggtc
     7081 gtggcaatga tcaatgcccc ttgctcatac atagattcca tgattgcgat cgctagtgca
     7141 gccccttcat ttggctctgt tccactgcca atctcatcta aaagtaccaa cgtattcctt
     7201 tttatctttt tcaaaatagc agcgatgttt tgcatatgac cagaaaatgt actcaacgca
     7261 ttttctaaat tttggtggtc accgacatcg acgaaaatct catcaaagac agcaagctct
     7321 gtcccttctt ttgccggaac ttgcataccg aacatcgcca tcaaagtcaa taaaccgact
     7381 gtcttcaaca cgactgtttt gcctccggca tttgctccgg tgataatcaa gcctcgatag
     7441 tctttaccga tttcaacatc caatggtacc gcatgctcag gtaaaaatgg atgtctgcct
     7501 tgcttgataa tgatatgttc cgatttattg attctaggtg tgcttccatt gatttctcgg
     7561 ctaaattttg cccgagcaaa gatgatatct aacaccgtga tcgtttcgat caataaatcg
     7621 atcacttctt cgttttctgc gatagcacca gtcaatgccg ccaggacttg atattcttca
     7681 ctgatttctt cagcttttaa caactgatag tgttcattca atttagaaat cacagttggt
     7741 tcaataaaaa ccgtcgttct tttatttgat tcatcgataa tcgttccatc tattttattt
     7801 ttatagctgg ctttgatagg aatcgtatag tattcgcctt tttgaacaat cattgcttct
     7861 tggatcattt ctttgttttt cggatgacgc aagaatttca gtaattttga ctggatttct
     7921 ttttcgatta gttgtaattg tttgcgtact ttacgcaaat tacgtgacgc gtcgtcactg
     7981 actttattat tttttatttt ctggtcaatc aactcttcta catttatcaa atcaggaaga
     8041 tgtttgctat aagcaaacaa caagggtgtc tggtattgat tcttatcaaa aaactttgtg
     8101 atcatccgac tgcttctaag aaaatccgca tattcgatca aatcggttgg ctgcagaatc
     8161 aacccttttt tgacttgttc tgtcagtgca tcgatcctcg gcaaccccat aaatggaaca
     8221 tgctgactgc tttctaagat caagcgtgct tctcttgttt cttcttgacg atccaagaca
     8281 gtttgtaggt ttgtcgagac cgtcatatcg ctgattcgtt ttttgctata gtttccaatt
     8341 gctcttgctt ggatttcctt tttgatccga tcaaattgta attgactaat catttcgttg
     8401 tccatattct ctccttttct tgaagagata cctgtcccaa aataaataag ggcctagaga
     8461 taaataaaag gatagaacac agcttaaaaa agtcatgtcc tatccttatt agaatacaaa
     8521 gatattgttt tcctgacagg taccgtttca taagcacacc aaataaactt tgtttattgg
     8581 ctattcgact tactgattag ataccagcta cactcacaaa acatacccca ttcttctagg
     8641 tcttatttat tttccactta actatagcaa gacacatttt ggttgtcaaa ctttagtatg
     8701 ttttatagat ttacaccatt aatgaataga cacctaccgc gataattgct ccgatcattg
     8761 gacctgcgat tggtacccat gaatatgccc aatctgaagt tcctttatta gctattggca
     8821 ataccgcatg tgctaatcga ggaccaaagt cccgagcagg gttgattgca tatcctgttg
     8881 gccctcctaa tgaaagaccg atggctaaga tcaatccacc tacagcgaat acgttcgttc
     8941 caggtgcaaa ttcgttttgt ccaaaagcta gcaacccaag aactaataca aatgtaccaa
     9001 ttaattctgt gatcacattt gctggataat tccgtatagc aggacctgtt gcaaacgttc
     9061 ctaaaatcgc gctttcatct ttcgttgcat tccaatgtgg caaataagaa agccaaacaa
     9121 gaatggcccc tgcaaaagct cctaaaactt gcgcaatgat gaaaggaacg acaagattcc
     9181 aactaattgc ccctgtcatc gccatagcta gagagactgc aggattcaaa tgagctgggc
     9241 ccatgaatcc agaaatatag acagccattg ttacagcgag tccccaacca aatgcgatga
     9301 caatccagcc agaagcctgt gctttacttt tgtttaaatt cacagccgca cacacaccat
     9361 ctccaagcaa aactaggatc agtgtcccaa taaactctcc catgatttga gtcatatcac
     9421 tgttcattta tttctccccc tttagttccc gcagatctga ttctgcaatc aatgcttcta
     9481 aacgtttagt ctgttgcgcc ttctcctctt ctgtccattc aaaatatcca gcaattgcat
     9541 cgatcacagg ttgcttgatc tcatcaagct gatctcgttc aaacaacaga tgatttgtac
     9601 gacgaatgag ataatcgcct ggggccaaaa ccatttcttc ttctaaacca taacgtaacc
     9661 gagctgactc agcgagactc aaaccaggat aaggtgccat ttcttttgct aattcaaaga
     9721 tacgtcgggc attagtcccg taaaaatcag cgatataagt agcatcttct tcctctaaac
     9781 cggctgctac tccttctttt gctaattctg tcactgtttc ttctaattta gttggatcga
     9841 agtttccacc tgaaatctga tattttttag agtcgatttc tttcgtctct attccgtatt
     9901 cttcttttaa taattggcgt atcaatcgca aagctccttc tgccatctta cggtaatcgg
     9961 tgattttccc accagataaa gtcagcaaac catctggttc tctttctaaa gaactacctc
    10021 tggaaatcgt agaaggtgct ttttcatcac ggctgttttc caaatggttc aacacgtctt
    10081 ctacttcagc acgagaaact ttgttttcct tatattcact tactgtatcg acaactttat
    10141 tgaagctctt gtctgaaatc gatccattat ctccaccatt gtaatcagaa ccagaattac
    10201 caatcaacag tggacgaagt cctgcccagc ttgcttcgat atcattcaat gtgatatttg
    10261 cttctggata gcgatgattg atcacatcca atagataatc tacatcttct tgtgtgactt
    10321 tcggatccgt aaaatctcct tggtaatccg tatctgtcgt accaaagtaa gtcttgtttt
    10381 ctcttggaat agcaaaaacc atccgcccat cttgctttcc tgtatcgaag tatgtgggtt
    10441 gcggtacagg cagttttttc gcatctacaa ctaaatggat ccctttagtt ggacgcattt
    10501 taggagagac tggacgcgta aaattcaagt ttcgtacttt atctacccaa ggaccactcg
    10561 tattgatcac taattttgct ttgatttcga tcacttcatc tgtcagcagg tcacgagctt
    10621 tgacgccaac gatttgatcg ccatcatata aaaagccaac cgctttcatt ttacttacta
    10681 gataagcccc atcttcggca gcctttttga tattatcaat cactaaacgg gcatcattgt
    10741 tgcggaaatc tagatagaca cccgcgcctt ttagcccttc ttttttcaaa aacggttctc
    10801 tttccaatac ttcttctggt gtcagcgtat aattctcata tttggttcct gtcacatttg
    10861 ccagtttgtc ataaaggtcc attgctactt tgaccgagaa catattgaag gttgttgctc
    10921 cttcgccctc atagattggc aaaagcattg gatctggttt tgggatgtgt ggggcaatac
    10981 tttgtacgac tgcacgttca ccaactgtgt cagctactac ttccacatca aatgtcttca
    11041 gataacgaat accgccatga acaagtttgg tcgagcgaga ggaagtccct tctgcaaaat
    11101 cctgcatttc gatcaatcct gttttgattc ctgatgctgc tgactggact gccacaccgg
    11161 caccagtgat tccgccaccg atgatcaaca gatccaattc ttcttgctgc attttttgaa
    11221 tgctatcttg tcttgtcttg tttgaaaaca tatggatttc ctccttattc aaatgcttgg
    11281 gtagctttta ctgctttttt ccaaccgttg taaagttttt ctcggcgctc ttcctccatt
    11341 tgaacttcaa atagcttgcc agcttcataa aattcgcgga tctcgtctgt atctttccaa
    11401 taaccgacag ctaatcctgc taaaaatgct gcccctagcg ccgttgtttc caagttttct
    11461 gctcgttgga tcggtacatt caagatatct gtttggaatt gcatcagata ttcattgttt
    11521 gctgcaccgc catctacttt caatacagga atcttgattc cagtgtcatc ttgcatcgta
    11581 tcgatgatat ctctcacttg ataagcaatc gactgtaaag tagctttaat gatatcttct
    11641 tttgtcgtac cacgtgtcaa gccaaacata gcccctcgtg ctgcctgatc ccaataagga
    11701 gcacctagcc caacgaatgc aggaacaaca taaacctcat cttcgcttgt agagcgtttc
    11761 gctgcgtctt ctgaatcgct agctttttgc aacatttgaa gtccatctct cagccattga
    11821 atcgaagaac cagccacaaa aatacttcct tctaaagcat agtaaacctt tccatttatg
    11881 ccgtaaccaa ttgtcgtcaa taaattattt ttggataatt gcggtttttc accggtattc
    11941 atgacaataa atgaacctgt accataagta ttcttgacca tgcctggttc gaatgccatt
    12001 tgtccgaata atgcggcttg ctggtctcct gccatccctg aaattggtgc ttcgcttcca
    12061 taaaaatgat aaccttgcgt ataaccataa acttctgagt tagaagtgac ttttggtaac
    12121 atcactcgag gaatatttag taaatctaaa atctcctgat cccaatctag ctgatggata
    12181 ttgaacaaca tcgtccgact ggcattcgag taatcagtaa catgtgcttg tccatcggtc
    12241 agtttccata ctaaccaaga atcgatcgtt ccaaacatca attcgccttt ttcagctcgt
    12301 tcttgcgcgc cttctacatg atcaagaatc cagcgtatct tcgttgctga gaaataggca
    12361 tcgatcacta accctgtttt ttcgtggaaa aattcttgat agccttcatt ttttaagtta
    12421 tctgcaattc cagctgactg tctggattgc caaacaactg catgatagat tggacgtcct
    12481 gtatcttttt cccatattac tgttgtttca cgctggttcg ttatgccaat cccagcaatc
    12541 tgatttggtt tgatccctga ttcgatgaac gctccggcaa taactgactg tacagaattc
    12601 cagatttcat ttgcatcatg ttccacccaa ctttcttgcg ggaaatattg cgtgaactct
    12661 ttttgagaac tgccgatgtg tcttcctttc ttgtcaaaga taattgctcg tgagctagtt
    12721 gtaccttggt caatcgccat gatatacgtt gattctgtca ttgtgattcc ctccattata
    12781 tgcaaacgct ttctttaact actcttatca tactactgtt ttaaaaaaag aaactgtcaa
    12841 aattttattc tttttttctt tttttgacag ttttttatta tgaagctatt ttttaatcaa
    12901 ttcttttctt ttttcagcgt ctctattctt cttttcaatc gaataatgtc gtaaggtgac
    12961 gcaaattctg acagataata ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc
    13021 aaatcatatt ctttaccttc ttcataactt ccaatctcaa taccgccgat tcccctcagt
    13081 tctctgatca gatattgttg aaaagcaata cgataaatcg gcagactttc taaatcgatt
    13141 ccaatcacta ctggttttgc aatataaaaa tcgatcatca aaagaacatt ggcatactgg
    13201 atcatcaggt aactaagctc actatcttca gctcttttcc tcggcaattg ctgcaccgct
    13261 gtttcctgta atttcgccag taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt
    13321 tccaaaagcc gatcacgctc atagacttcg atctttccaa caaagaaaaa ccattcattg
    13381 ttgatctggg aaatttggta gcctactgcc ttttcttctt tgattttcaa acgacgagga
    13441 cgataatgcc ataacatcgt ttcccgaatg taagtatcta aaacagcggt tggtaacttt
    13501 ttgctccgtg tcaaatcata ctgataaaat atttcttcgt ctactatcga aaaagaaaca
    13561 aaaaagctgt aaaacatcat tgtttcgtat acattcagtt ctgctgctgt tcgactaaga
    13621 tgcatcacga tgattggatc gattgcttga taaagccggt ccgactgata gagtaatttc
    13681 ttttctttca aagttgcgta cgtacttttt tcattcaata gtcttttttt cgtaatcccg
    13741 agccagcaag caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc
    13801 aatcctagaa tgaacggtct tttttctggt gtattttgca aaaaaagcgg tcgctgatct
    13861 tccgggatat attggaatag ttcataataa aaatatcgta tctgtagctc ttctccgtat
    13921 aattgaccgt ttttgatttg taatccaaat tcctctaaga gctggttcaa ttctttgatc
    13981 tttcgaaaaa cagaagactc actgatatta aacgctgtcg ctaatcgaac gatggatact
    14041 tgctgatgtt ctaataaata gacgagcatc ttaaacttca agctttcctg caataaaaac
    14101 aatagaattt tttccaaact tacatcttcg tccaacacaa gcagcgcctg atactcattt
    14161 cgctgtactt ctactttttt ttccatcatt cggccaatca attcaatatc ttccaaatat
    14221 tgatcaaacg aggcttttga cagttggaca tgctctctca aaccattgat agaggctgtt
    14281 cccccttgca gaacaaggtt tcgcaaaatc aggatctgta actgctcttt tctttctaat
    14341 aatgcttcaa tcttcatagg cagtattcgc cacctttgac cacaccatat ctggcacacg
    14401 gtattctggc tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc atccttcttc
    14461 tactgcaatt gtcagttggt caagaaggtg gaacatctct tttttgcttt ggagctgtgc
    14521 tccaagaatc tttttttcag aatcaaatag aaatttgagt ttaactttgc tttgatttat
    14581 tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt tggggataaa aaatgctctc
    14641 ttcttctgtc aaacctacgc ttcctaagaa atagccaaac cactgattgc cgactggacg
    14701 ataggttccc tgatcctttg tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt
    14761 acggacagcg ttatttacta gagaaacata cattttttca tttgttggac gaaaagttac
    14821 ttggatcgca tcgccaatcg cataaacgtc tttttgagac gtttgcaagt agtcatctac
    14881 ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc cacatctgat tgtcggggcg
    14941 agaatgatta gccagcaaaa caagatcata gttctccttt cttttttccg tctctacaat
    15001 agcggtttca ttttcggtca aagacacaac ttgttcttct aaaaaaagtg aaagcccttg
    15061 atttttcatt tcctttacta acggttctgt catttcagga tctagataac gaaaaagaat
    15121 tgactttcgg ctttcgtaaa gatggatttg tttttttctt gatgccaacc cttcagctat
    15181 ttcgattcct gcttgtcctg caccaataat tgcaattttc tttgcattca tgatcttttg
    15241 ctgaagagcc gttacgtttt gtatcttttt aactgggtga ataagcgttg attctgcaga
    15301 aatatttcga aacttttgat ttgaaccagt cgctagaatc aaacgatcaa aatccaattt
    15361 ttttcctcct gctaaaacga cttttttttc tttcaatcct attactgtct tttctgtgta
    15421 aacatggatt cgatagcggt cagtcagttc ttctttcgtg atccaagaaa gttgtttttc
    15481 actggtaacc tttcctttta atatcaaatt gaacccgctg ggaatccatc ctaacttcga
    15541 ttgcttttca atcaaatata tttcacttgt tggcatcaat tggcgaagat aaatagccgc
    15601 atgaatccct gcaaacgacc cgccgacaat tacaatgttc atctgcattc ctctttctta
    15661 taaggtgttc tactgctaac ttctcaatca gaaaacacag tattcataac tcgctttcaa
    15721 ccttatgata catagcgatg cagcagaatc aaccctctcc atcataccgt gttctaggtc
    15781 tgcttctcaa taatgtacta gtggacctaa ttgaagagta tcatttctga agagatgttt
    15841 caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag
    15901 gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt tagaatagac
    15961 acatcattta tttttaattc tttaatgctt caacaactgc ttcgcctaat tgatgagcag
    16021 attgaggatt ttgccctgta agaagccgcc catcaatttg aaccacttct gaaaaagctg
    16081 ctccagactg atagtttgct cctttttctt tcaaagcatc ttctgctaag aatggtactt
    16141 cattcgttgt accatttgct tcctcttctt cattactgaa cccagtcact gtacgtccag
    16201 ctaaaataga ttttccagac tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa
    16261 cagctgagac cagcccttca tttcgataga tttgtgaagc gactttccca agatctattg
    16321 cttctggaaa atcccacatt gttccgtggc caccagcata ataaaccgct gcataatcat
    16381 ttgcatccac atcatcagga cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat
    16441 cttcataata ggcccaatcg atttcatcca tattcaaaac actttgcggt tctaaaggta
    16501 catatccgcc tttaggactg acaaaatctg catcgatccc attttttact aatacatcat
    16561 aaaaatgagt cagttcactt aaccacaaac ctgtaggacg ctcgatcttt tcgtactttt
    16621 ctacatttgt taatacgatc aatactttca ttttcccatc ctctttctat aaaaattgcg
    16681 ttattgataa cgtgctttca agaaattata taatgggctt ggcaaataaa gctctttata
    16741 tgcttcttca aaaggtttcg ttacaaattc atcctcacgg ttttccaata atttctctgc
    16801 ccagtcagga tcgacgatca tttgctgtcc aattgctgca agttctgcat atttcaatac
    16861 ctgctctaca tcttcgcgat tgcggatccc accgacacca actaaaggga tctttccttg
    16921 aatcgtgtta tggaccactt caagaatcga tttatcgttg tatttatcag aacgagctat
    16981 tcgatcatac atatttaatg aaacatgcag ataatctagt tttgactcac gtaatcgttc
    17041 aagaagccag atcgtgtctt caaaacggat acctggtgtt tcatattctt ctggtgagaa
    17101 acgataccct acgatgaatg gttttttagc atattgttca accgctgaga aaaccgttgt
    17161 taataattct tcaataaaac gataacgatt ttccaagcta ccgccccatt gatcctctcg
    17221 tctattcgaa tgcggagaga aaaattgttg gatcaaatac gtatttgctc catgcaactc
    17281 gattccatca aatcctgatt caatcgctct acgtgtggct ttgccaaatg cttggattgt
    17341 ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc gcatcaggac gttttgctgc
    17401 aactgcactt gcagatacag gctgttctcc agataaagtt gctcgacttg tcattcgtcc
    17461 accgtggaaa atctgaataa tcgcttttgc gccttctcct tggattgctt tcgctagctc
    17521 acttaatcga ggaatcattt catcatgagc gatgctgagt tcacctggcc agccttttcc
    17581 gatatcttgg acatttgcag caccagtaat aacagcacca agacctttag aacgttgttt
    17641 ataataagtg atctcatctt cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat
    17701 aggcgacagc atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc
    17761 aaattgtgac attgtcattc ctcccataat tcgctctgat taacctatcg agaatcatct
    17821 atcattaaac cactcgatta gttgtacatg caactaattt gcttaaaaaa ttttattttt
    17881 tttgtaactt atctgttgca tcattcattg tctgggctaa ttctttactt gcttcatttc
    17941 ccaaaactgc tttgtatgac tgatgaagtt cttcccagct accatcgact tccttagcaa
    18001 attggcgtcc ttgttctgta agataaacta atgacaagcg accttcgcta actgttttga
    18061 cgtatccttt atattctaat ttttcaacaa aacgagcaat cgttgatggg gcggaataga
    18121 gcatttgtgc aatatctttt tgtggtattc catctttttc ttttaatatc aataaaataa
    18181 aaccataagt tggtgccaga cctgtccttc taaacgcttc atcagctaat ttattcaaca
    18241 tgcgatctag ctttttcacg ctaaagaaaa gacattcatc taacgctttt tcaattggct
    18301 tttccatttc atcccctcca ttggcgtaca tatcatagta tgatttttat tttttgtcaa
    18361 aaaacacgga ataatgtccg cctcctctgt ctagatttga aaaaaagaag tagccatctt
    18421 caaagaattt cggcttgttt tcgacaaaac tccttctgga acaccgtgaa tcagaggttg
    18481 tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg tgaaaatggc tttccacatt
    18541 tttgcataaa tttggcttaa taccgaaaaa ctggcttttg aacaccgttt atatcagagg
    18601 ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
    18661 attttgtcgt tttttgagct aatgccgatg gtcagctgat tgtacaccgt ttattgagag
    18721 gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca
    18781 tgttttatga ctatggtgac ttgtgatcga ggagttggca ggagaatact gtttattcgt
    18841 aaagaagggg ctgtgacaag acttttgtca cagtccctta tttcctttta ttataggatt
    18901 tcgccatttg attggatgac ttgtctgtac caatcaaatg attttttctt taaccgtttc
    18961 tttgtgccat tcccttcgtc atcttgatca acatagatat atccataacg ttttgacatt
    19021 tgtgaagtag aagcgctgac gatatcaatg cagccccacg acgtataacc aaataaatct
    19081 actccatctt tgatagcttc cttcatctgc tcaatatgct gtttcaaata gtcaatgcgg
    19141 taatcgtctt gaacggttcc atcttcttct actttgtcta tagctccgag tccgttttca
    19201 gctatgaaca gtggtttttg atacctatca tataattgat tcaaagcaat tctcaaaccc
    19261 ttaggatcaa tctgccagcc ccattcactt gatggaagat acggattttt aattcctcca
    19321 gttattaagt ttccttggac cccttcccaa ttttctggat gagcactgac tgcactagac
    19381 atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc ttcatcacct
    19441 acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata cgctggataa
    19501 tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc aattgttttt
    19561 tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat catacaaccg
    19621 acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact tgctataaac
    19681 tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc ttcaattact
    19741 cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt catccaatat
    19801 tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta aaactgaata
    19861 agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat ttcataatgg
    19921 gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac ttgatcataa
    19981 aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat tcttgtccat
    20041 gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat gtcttcttta
    20101 aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct gttgggatcc
    20161 tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca tttttcttta
    20221 ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc atctacatcc
    20281 catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat
    20341 tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt ttcttatgac
    20401 tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg gtcggatcat
    20461 atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct ttacgtactt
    20521 tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact gtatcaatac
    20581 cgatatggat cagtatctct gctccttctt cactgactac cccaattgca tgtttcgttg
    20641 gaaaaactgt tgaaacttcc ccgtttacag gactgtagat ttttccgtcc gttggaatga
    20701 tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact tcagataaat
    20761 caattatttc acctgctgca ggagtactta acactatatc ctttgaggat tcttcttcga
    20821 tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc gctaagaaat
    20881 aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac aaaatacttc
    20941 ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca aatatagctc
    21001 cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca taaacagcag
    21061 gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt tttactttct
    21121 tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct acggcagtca
    21181 tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc attactggta
    21241 caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg ataacagcac
    21301 cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc agctgacttg
    21361 cataatctgc aattggacct acaactaata aagtaattgg gaccatcacg atgatacaaa
    21421 tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa
    21481 tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta taccttgcta
    21541 gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca ctgacaatcg
    21601 taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta ttgaactttt
    21661 ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca gctgctgcat
    21721 tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta cataaggcga
    21781 gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca aaaacagcgg
    21841 cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc tttccactag
    21901 atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca tttactgcat
    21961 ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca ccttctagtt
    22021 gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt aaccttgttg
    22081 cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca atatctttcg
    22141 ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta gcaatccttc
    22201 ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta ccccttataa
    22261 tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa tcactcactg
    22321 cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct
    22381 tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg atttttgttt
    22441 ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga agcataacta
    22501 ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg atttttattt
    22561 gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct tcaactatgg
    22621 tcagcttttc acgcaaaatc tgataaatct cccaagattc aaactcatct agaacactag
    22681 gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc aaaattgcat
    22741 catattccgt tattttgttt gattgatttt tgaaaagata cactggttct atccggcatt
    22801 ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc aaaatactaa
    22861 ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc aacgcttcgg
    22921 caagatacaa aacaaaataa gcgctttcag ctaaattggg acgtaattga aaatgtattg
    22981 ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc ttttgtacaa
    23041 aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta
    23101 gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg
    23161 tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca
    23221 aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat
    23281 agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat
    23341 acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag
    23401 aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta
    23461 atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc
    23521 ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt
    23581 cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc
    23641 gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga
    23701 aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa
    23761 tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga
    23821 tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact
    23881 ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt
    23941 tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc
    24001 ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct
    24061 ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa
    24121 aatcatttga agaaagatct atattcaagg tttttaccat tagactggat aatttgttta
    24181 taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta
    24241 tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg
    24301 attggtcccc accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt
    24361 tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca
    24421 tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg
    24481 tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt
    24541 tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg
    24601 cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct
    24661 gccgtgttag cttgcaagca ctgttcgtct ccatctaaca ttactggtgc acagtcttct
    24721 tccttccatg tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa
    24781 aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat
    24841 gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt
    24901 ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa
    24961 atcacttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc
    25021 aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa
    25081 tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg
    25141 attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg ggtttcataa
    25201 tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca
    25261 taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc
    25321 catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct
    25381 tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa
    25441 gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc
    25501 ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat
    25561 tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata
    25621 actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca
    25681 agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt
    25741 aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc
    25801 taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt
    25861 gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg
    25921 aacagtgaac gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc
    25981 atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc
    26041 ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac
    26101 agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg gattgactcg
    26161 aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt
    26221 cctagcattg tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc
    26281 agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc
    26341 atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt
    26401 catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt
    26461 tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc
    26521 ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa
    26581 gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg
    26641 gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt
    26701 tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat
    26761 ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt
    26821 gccaatcata tcgatcacgt ccatacgaaa gcccccaata cctttatcga tccagaaatt
    26881 catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg
    26941 cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc
    27001 actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata
    27061 atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc
    27121 agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc
    27181 aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact
    27241 gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc
    27301 aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc
    27361 atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca
    27421 ccatttttgt tccatgattt tcctcctata aatggatgtt tctatttttt gcttaatcat
    27481 ttattttagt ctctctcact aaaaattgta acgctttcaa atagctattg caccttgtta
    27541 tcggtaacag aataaaacaa aaaaacagct gaggaaattc cctcaactgt tttttgaaag
    27601 gattgattta tgcagggaag aatgctgatt tcacaacttc tttataaacg gtgttcactc
    27661 agctgtccct tggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttcgtttt
    27721 tttctctaat actcaggcca gaacactgat ttcacaacct ctttataaac ggtgttcact
    27781 cagctgtccc tcggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt
    27841 ttttctctaa tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata
    27901 tccacatgaa tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc
    27961 ctcttttagc tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg
    28021 aaatactata agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta
    28081 agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg
    28141 ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca
    28201 agaagaaaat ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag
    28261 ctgatgattc tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag
    28321 ctgttgaatc agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac
    28381 ctgcttggtt tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga
    28441 atgtcagcac tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa
    28501 ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca
    28561 aacctgtgac cagacctaaa atcgttgtag ctgctatccc gatgaagatg ccacctggta
    28621 cacgccgaac aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac
    28681 caacgttgag tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt
    28741 cgctcagacc aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat
    28801 ctgctgggat cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa
    28861 tcaaagaagc aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac
    28921 aaacagaata agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac
    28981 gggctaatac ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca
    29041 ctgctccttc atccattcca gaagcaccta atacagttgg atttacaaat aagatataag
    29101 ccattgagat aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt
    29161 tttctatttc gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac
    29221 attataaata aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat
    29281 actaaaacaa aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca
    29341 tctgattaaa tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc
    29401 tttattgtgt taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt
    29461 ttaccatttc gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta
    29521 tttagtccgc caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct
    29581 gatttgagat tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa
    29641 ccttctaatc ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc
    29701 agcttttcct ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc
    29761 attgttatct gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc
    29821 cgtaaaaaat actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa
    29881 tacttttggg aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa
    29941 ttcttctggc aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg
    30001 gcaaatcatc tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg
    30061 acccttcttt ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa
    30121 gcgcaaacac cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg
    30181 ctttcctcca caaacttttt ttacaaaaaa aaaaaacggc aatgaatgcc gctcttctta
    30241 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    30301 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    30361 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    30421 agcattgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    30481 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    30541 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    30601 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    30661 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    30721 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    30781 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    30841 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    30901 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    30961 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    31021 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    31081 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    31141 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    31201 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    31261 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    31321 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    31381 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    31441 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    31501 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    31561 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    31621 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    31681 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    31741 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    31801 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    31861 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    31921 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    31981 attttgtttg taagactagt ttacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    32041 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    32101 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    32161 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    32221 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    32281 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    32341 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    32401 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    32461 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    32521 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    32581 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    32641 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    32701 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    32761 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    32821 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    32881 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    32941 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    33001 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    33061 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    33121 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    33181 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    33241 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    33301 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    33361 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttga
    33421 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    33481 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    33541 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    33601 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    33661 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    33721 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    33781 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    33841 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    33901 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    33961 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    34021 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    34081 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    34141 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    34201 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    34261 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    34321 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    34381 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    34441 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    34501 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    34561 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    34621 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    34681 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    34741 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    34801 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    34861 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    34921 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    34981 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    35041 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    35101 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    35161 catcggttgc tgtagcattg atgaatccca tcgaattaaa gatcgttact agcaaagcgg
    35221 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    35281 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    35341 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    35401 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    35461 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    35521 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    35581 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    35641 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    35701 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    35761 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    35821 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    35881 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    35941 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    36001 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    36061 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    36121 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    36181 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    36241 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    36301 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    36361 cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta tttagataac
    36421 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
    36481 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
    36541 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
    36601 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
    36661 aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt tcatctttgt
    36721 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
    36781 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
    36841 ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa aaataatctt
    36901 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
    36961 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
    37021 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
    37081 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
    37141 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
    37201 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
    37261 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
    37321 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
    37381 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
    37441 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
    37501 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
    37561 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
    37621 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
    37681 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
    37741 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
    37801 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
    37861 taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
    37921 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
    37981 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
    38041 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
    38101 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
    38161 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
    38221 gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga ttttttgagc
    38281 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
    38341 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
    38401 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
    38461 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
    38521 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
    38581 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
    38641 caacaaaaaa actttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
    38701 gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga actcacttgc
    38761 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
    38821 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
    38881 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
    38941 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
    39001 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    39061 aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat cgcagatgca
    39121 agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct tgagcgataa
    39181 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
    39241 ctatctctgg cacactgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
    39301 ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc ggcttgccat
    39361 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
    39421 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
    39481 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
    39541 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
    39601 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
    39661 caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc tttcttttct
    39721 ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt ttgtctttta
    39781 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaat
    39841 cccgtttgct cataactaga aggacctgta cctggaccat aatacccata agaatctctg
    39901 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa tgaacctctg
    39961 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
    40021 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
    40081 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata gccttgctaa
    40141 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
    40201 cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct atcttcagca
    40261 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
    40321 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
    40381 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
    40441 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
    40501 ctagctcctt agctgcttgg attgcaagcc tgatatgcgg ataccctaat atcgcttctt
    40561 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
    40621 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
    40681 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
    40741 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
    40801 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca tgatgccatg
    40861 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta agaaataaag
    40921 catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca ggttttggac
    40981 ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc tgagtcactg
    41041 ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat tacttgctga
    41101 aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca aatgcacaac
    41161 ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc agccaactat
    41221 ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac gtacgataag
    41281 cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca atcattttgg
    41341 aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg atcgtattca
    41401 agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa
    41461 agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc aaatattgat
    41521 agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta gcaacaaacc
    41581 gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt tgatagaaca
    41641 gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca tattttttat
    41701 tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg atctgcagca
    41761 tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc agtaaaaagc
    41821 ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa agataagccg
    41881 tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa aattcccaat
    41941 aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata aacattggtg
    42001 ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc
    42061 tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca gctgatactt
    42121 tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct ttctctctct
    42181 ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt tcaatagaat
    42241 ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc gagtcactac
    42301 ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa ttggcaattc
    42361 aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta ctgccggaat
    42421 cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc ctgataatat
    42481 gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct cttgtctata
    42541 cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat gttttgagat
    42601 ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc
    42661 gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa cgacccattc
    42721 aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat gtgcttgaat
    42781 cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg tttcgacacg
    42841 tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt
    42901 gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat tttgttctga
    42961 agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt ttgtataatt
    43021 attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt cagcaaccga
    43081 acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa tttctttatt
    43141 gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc
    43201 atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg cataagcagc
    43261 agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc cgtatgcttg
    43321 gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt tatcttcttc
    43381 gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt
    43441 tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga tggcttcttc
    43501 tatgaattta cttgatttac cataatacac agtactaata gaagaagttg gatttttcat
    43561 gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt catttccttc
    43621 tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt cattgtaatc
    43681 gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat aaaatgctga
    43741 gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag cagctttgat
    43801 tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc gttgacgaga
    43861 tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa cgcctgcttc
    43921 ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg cgattaaata
    43981 actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc ctggaatcaa
    44041 accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt ctgaaataga
    44101 taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta caatgactgg
    44161 aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag caacgatttt
    44221 tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag cgattactac
    44281 tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac tttcagggac
    44341 gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa tcaattcacg
    44401 tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa aactattttt
    44461 catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct ggtgtagcac
    44521 cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat ctcgttaata
    44581 cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca gtagattctg
    44641 ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact cctacatgga
    44701 ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc atggatctct
    44761 cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc ttccatctgg
    44821 gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga atttcattgg
    44881 tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg cagctaataa
    44941 tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca cgatgccggc
    45001 tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg taacaccgcc
    45061 aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca aataagcaat
    45121 tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg gtgcattggc
    45181 taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga ctggtttttt
    45241 atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc ctaatggatc
    45301 atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc caggattatg
    45361 tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg tagaagagtt
    45421 tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat attgtcctgc
    45481 agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc
    45541 tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat ggatgctttc
    45601 aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata cccagttctt
    45661 cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca attcgatagc
    45721 tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg cgtagattcc
    45781 tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag gatctcgtgt
    45841 gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta cccagtcacc
    45901 agggatatct acacgtggta aagaatcgac aacttcgttg acttgaacga tcacgatacc
    45961 atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga aacctgtgta
    46021 caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg gaatcaaatc
    46081 gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt tcattgtgcc
    46141 gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga aatcgatctt
    46201 gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac tagacatgat
    46261 caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg aaagaaaact
    46321 tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa tcagtttttc
    46381 cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt tgctgatttt
    46441 ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg gtttttccat
    46501 aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat gttggtctag
    46561 attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct ttgtcaaagt
    46621 attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca agtccatcat
    46681 ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa tctcttctgc
    46741 gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat cagatagatg
    46801 gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga gacagtgaga
    46861 tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa
    46921 cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac aattttcatt
    46981 tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct cagcttcgct
    47041 tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt ctttcattaa
    47101 gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc gacaagcaac
    47161 agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt
    47221 ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga taaaaaccgt
    47281 atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt ctaaacgttc
    47341 catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt gtccaggaaa
    47401 taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca ctgctatctt
    47461 agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat
    47521 ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa
    47581 caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc ttgagccgtg
    47641 cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc ggcagaaaca
    47701 ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca ttagacacat
    47761 tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat
    47821 tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt atggaaaagc
    47881 atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt accgatcctg
    47941 tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc atgaaagaac
    48001 ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat ctgcaattag
    48061 aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc cgtcatacac
    48121 tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg acagatgatc
    48181 ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc ccaaaagttt
    48241 cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat ttgcccttga
    48301 cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc aaaatgattg
    48361 caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg gacaaaattc
    48421 ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta gaaaataagc
    48481 cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    48541 aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt tgatttaaaa
    48601 cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag atcaatagag
    48661 atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc tcgcaaaatt
    48721 ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg gtcacttgca
    48781 tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt tttgcttttt
    48841 tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg agacaaattg
    48901 tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt ccatctcttt
    48961 cttattgatt cgctttattc aaaga
//
LOCUS       DACDKL010000006        42860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDKL010000006 DACDKL010000000
VERSION     DACDKL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.006"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..2342
                     /locus_tag="IV329_000254"
     CDS             6..2342
                     /locus_tag="IV329_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8939462.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(2457..2768)
                     /locus_tag="IV329_000255"
     CDS             complement(2457..2768)
                     /locus_tag="IV329_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939463.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            2949..4424
                     /locus_tag="IV329_000256"
     CDS             2949..4424
                     /locus_tag="IV329_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8939464.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            4471..5055
                     /locus_tag="IV329_000257"
     CDS             4471..5055
                     /locus_tag="IV329_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8939465.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            5057..5476
                     /locus_tag="IV329_000258"
     CDS             5057..5476
                     /locus_tag="IV329_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8939466.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(5757..6041)
                     /locus_tag="IV329_000259"
     CDS             complement(5757..6041)
                     /locus_tag="IV329_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8939467.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(6393..7103)
                     /locus_tag="IV329_000260"
     CDS             complement(6393..7103)
                     /locus_tag="IV329_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8939468.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(7100..8572)
                     /locus_tag="IV329_000261"
     CDS             complement(7100..8572)
                     /locus_tag="IV329_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8939469.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            8710..9183
                     /locus_tag="IV329_000262"
     CDS             8710..9183
                     /locus_tag="IV329_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8939470.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(9336..9887)
                     /locus_tag="IV329_000263"
     CDS             complement(9336..9887)
                     /locus_tag="IV329_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8939471.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            9992..10522
                     /locus_tag="IV329_000264"
     CDS             9992..10522
                     /locus_tag="IV329_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8939472.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(11005..11448)
                     /locus_tag="IV329_000265"
     CDS             complement(11005..11448)
                     /locus_tag="IV329_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939473.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(11464..12624)
                     /locus_tag="IV329_000266"
     CDS             complement(11464..12624)
                     /locus_tag="IV329_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8939474.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13131..14831
                     /locus_tag="IV329_000267"
     CDS             13131..14831
                     /locus_tag="IV329_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939475.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(14933..15766)
                     /locus_tag="IV329_000268"
     CDS             complement(14933..15766)
                     /locus_tag="IV329_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8939476.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(16070..18235)
                     /locus_tag="IV329_000269"
     CDS             complement(16070..18235)
                     /locus_tag="IV329_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8939477.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            18495..20789
                     /locus_tag="IV329_000270"
     CDS             18495..20789
                     /locus_tag="IV329_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8939478.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            20782..21447
                     /gene="pgmB"
                     /locus_tag="IV329_000271"
     CDS             20782..21447
                     /gene="pgmB"
                     /locus_tag="IV329_000271"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8939479.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            21440..22447
                     /locus_tag="IV329_000272"
     CDS             21440..22447
                     /locus_tag="IV329_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8939480.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            22469..23488
                     /locus_tag="IV329_000273"
     CDS             22469..23488
                     /locus_tag="IV329_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8939481.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(23769..25136)
                     /gene="nhaC"
                     /locus_tag="IV329_000274"
     CDS             complement(23769..25136)
                     /gene="nhaC"
                     /locus_tag="IV329_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8939482.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(25246..26667)
                     /locus_tag="IV329_000275"
     CDS             complement(25246..26667)
                     /locus_tag="IV329_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8939483.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(26910..28787)
                     /gene="tdc"
                     /locus_tag="IV329_000276"
     CDS             complement(26910..28787)
                     /gene="tdc"
                     /locus_tag="IV329_000276"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8939484.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(29078..30334)
                     /locus_tag="IV329_000277"
     CDS             complement(29078..30334)
                     /locus_tag="IV329_000277"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8939485.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            30914..32215
                     /locus_tag="IV329_000278"
     CDS             30914..32215
                     /locus_tag="IV329_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8939486.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(32284..32883)
                     /locus_tag="IV329_000279"
     CDS             complement(32284..32883)
                     /locus_tag="IV329_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8939487.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            33137..34018
                     /locus_tag="IV329_000280"
     CDS             33137..34018
                     /locus_tag="IV329_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939488.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            34031..35641
                     /locus_tag="IV329_000281"
     CDS             34031..35641
                     /locus_tag="IV329_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8939489.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(35718..36377)
                     /locus_tag="IV329_000282"
     CDS             complement(35718..36377)
                     /locus_tag="IV329_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8939490.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(36660..37325)
                     /locus_tag="IV329_000283"
     CDS             complement(36660..37325)
                     /locus_tag="IV329_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939491.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(37472..38077)
                     /gene="dhaL"
                     /locus_tag="IV329_000284"
     CDS             complement(37472..38077)
                     /gene="dhaL"
                     /locus_tag="IV329_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8939492.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(38105..39088)
                     /gene="dhaK"
                     /locus_tag="IV329_000285"
     CDS             complement(38105..39088)
                     /gene="dhaK"
                     /locus_tag="IV329_000285"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8939493.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(39090..39485)
                     /gene="dhaM"
                     /locus_tag="IV329_000286"
     CDS             complement(39090..39485)
                     /gene="dhaM"
                     /locus_tag="IV329_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8939494.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            39639..39959
                     /locus_tag="IV329_000287"
     CDS             39639..39959
                     /locus_tag="IV329_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8939495.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(40071..40940)
                     /locus_tag="IV329_000288"
     CDS             complement(40071..40940)
                     /locus_tag="IV329_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8939496.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(41036..42310)
                     /locus_tag="IV329_000289"
     CDS             complement(41036..42310)
                     /locus_tag="IV329_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8939497.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(42336..42845)
                     /locus_tag="IV329_000290"
     CDS             complement(42336..42845)
                     /locus_tag="IV329_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939498.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
BASE COUNT        12818 a         8797 c         7813 g        13432 t
ORIGIN      
        1 tgattatgct tcaattaaaa gatataaaaa aatattataa agtcggcgaa accaccacca
       61 aagcattgga tggtgtgtct gtcgctttca gacaaaaaga attcgtagcg attttaggtc
      121 ctagtggttc tggtaaaacg actatgctga atgtgattgg tggactagat aattatgatt
      181 ccggtgatat ggtaatcaac ggaaagtcta ctaaagactt taaggacagt gactgggatg
      241 cctaccgaaa caactctatc ggtttcattt ttcaaagtta caacttgatt ggtcatctag
      301 gcattattga aaacgttgaa cttgggatga cattaagtgg tgtttctaaa gacgaaaaac
      361 gaaaacgggc agaagatgca ttgcaccgag tcggcttgac agatcatatg cataaaaagc
      421 caaatcagtt atctggtgga caaatgcagc gagtggccat cgctcgagca ctagccaatg
      481 acccagatat ccttttgtgc gatgaaccaa ccggagcgtt agatacagaa acaagtatcc
      541 agatcatgga attgatccaa gagttatcga aagaaaaatt agtgatcatg gtcacacata
      601 atcctgaact ggcccatcaa tacgcagatc ggatcattga attttctgat ggtaagatct
      661 taacagattc ccatcctcac atcgaacgac caaaagatga tcaatttaac ttacgcagaa
      721 caaagatgag tttttggaca gcgttaaagc tttccttcaa caatatccga acaaaaaaag
      781 gacgtacttt cttgacctcc tttgcttcca gtattgggat catcggaatc gcgatcgttc
      841 tgtccctttc ttctggattc caaaaacaaa tcgataacac gcaagctgaa acgatggcga
      901 aatttccgat tactatttct aaggtaacga ctaaccagac tcgtgacgat gccggattgg
      961 gtgcaagcaa agcggattat ccagacagca aaacgattac tgcaaaagtc agcgaagaag
     1021 ataaagcaca gcacacgaat aagattgatc aaacatacgt cgattacgta acagacatcg
     1081 atcctaactt aagtaataat atcggattta cccgtactac aggaatcaat cttttacgtg
     1141 atgtaaatgg caaagtacag cctgtcagct tctcgaatca aaatcctgat acggaatctc
     1201 tttctttatc tagtacgatg tcagcaatga caggtgtggg agtgtcaagt ttccctacac
     1261 agcttgacac tagcaaagaa aactttttaa aagacaatta ttctttactt gccggaagtt
     1321 atccagcctc agcaacagat gtcgtgctga tcgtagacgg caataataat actaatatca
     1381 acgcattgaa gaatcttgga tttgatgtga aagaagacga aaaacttgat tttgatgata
     1441 tcgttggtac aacattcaaa ttagtaaaca acaatactta ctatacaaaa cttccaactg
     1501 ggaattttat ccctaataca gattatgatg cgatgtatca gaatgcttct gatgaattga
     1561 agatctcggg gatcctacgc gtgaaatctt cttctacaat gaatctctta tctccaggaa
     1621 tcgcttatag cgatcaatta acgactcaaa tcgtcaacga aaacaaagaa tcagaaatcg
     1681 tcaaagctca aagagacagt ggtgtaaatg tcttaactac tgaaaaagtt gacgaaaatg
     1741 ccaaacaaac attgcttagt tatttaggtg gagacagctt gccttcaagt atcatgattt
     1801 atccgaacaa ttttgaagac aaagaaaaga tcttggatta tctagatgat tacaataaag
     1861 gcaaatcaga tgaagataaa atcatttata ctgatttagc tggaacgatg actgaactga
     1921 ctggcggatt gatggatgct atcacttatg tccttattgc ttttgcaggg atctctttag
     1981 taacaagtat gatcatgatc agtatcatta cttatacatc tgtgattgaa cggacaaaag
     2041 aaatcggtgt actaaaagct ttaggtgcac gtaaaaaaga catcactcgc gtatttgacg
     2101 cagaaacgtg tattttaggt atttcttctg ggatattagg tgtgtttatt gcatggctag
     2161 ctacgttccc tatcaatagc atcttataca acatgacgga tctaaaaaat gttgctcaac
     2221 taaatcctgt ccatgcgatt attctagtga tcgtctctac tatattgacg atgcttggcg
     2281 gtcatcttcc tgcacgaatg gcagcgaaaa aagacgccgc tatcgctttg cgtgcagaat
     2341 aaggttgtga caaccacatt tagatttgag tcataagagc gctgaaatag tagaaaacag
     2401 caagaaacga ggaagtaata tgttccttgt ttcgcgctgt tttttttaat tattttttat
     2461 aattctggta tacttttctt tcctttgtac cacaactctt ctggcgtata cctgtctttt
     2521 ccatttatga atactgttcc atctgcttca atcgttactt cagtcccaat ccacatatta
     2581 cgagaaagtg cgagaagatt ttttcttttt cctcttcctt tttttccaaa ttgcaaggca
     2641 tcggttatct cttctgtcca atgagatttc acgtatttta cttctccgtt ttcaaatgta
     2701 actttcaaaa tattcgctat attttccact tttactgctt taggaaatga ctccttcatt
     2761 tttttcactt tccttcctcc aacactaagt atgcgctttc ttttagtgat aagtatatca
     2821 tagtttattc tattatcaaa aaaacgcatc cattccattg tagaatactt taccgaacaa
     2881 gttgaattgt tttcctcttt ttcacctttt actatagata ggtaaagaca actaaggagg
     2941 aatctctcat gacagatcaa ccgtatgatt taagaaaagt catcgaagaa ctaaaaacac
     3001 ttccaggtca atatcacgaa acagatattg aaatcgatcc tgaagcagaa ttatctggtg
     3061 tttaccgtta tatcggtgct ggcggtacag taaaacgtcc gactcaagaa ggaccagcta
     3121 tgactttcaa caatatcaaa ggtttccccg gcacacgtgt gaacatcgga acaatggcta
     3181 gtcgtaaacg tgtaggtgaa attttgcacc atgattatcg aacacttggt cgtttcttga
     3241 aagacgctgt agaaaatcca atcaaacctg tgaaagtaga caaagcagac gctccagcac
     3301 aagaagtcgt tcatctagct accgatgcag acttcgatat tcgcaacatt ttgcctgcac
     3361 ctacaaatac tcctgaagat gctggtccat acatcacaat gggtgtagtc ttaggatcag
     3421 atccagaaaa aacaatgagc gatgtaacga tccatcgtat ggttctagaa gataaagata
     3481 cgatcggtat gtacatcatg cctggcggac gccacattgg tcacttccaa aaacaattcg
     3541 aagccttgaa taaaccaatg cctatcacta tcaatattgg tttagatcca gcaattgcca
     3601 ttggcacgac tttcgaacca cctaccactc cacttggcta caatgaacta tggtgtgcag
     3661 gagctttgcg taacgaacct attcaaatcg ttgatggtat agcagtcgat gaagtgggta
     3721 ttgctcgtgc tgaattcatc attgaagcag aaatcatgcc aaatgaaaca atgcaagaag
     3781 atatcaacac gaactcagga aaagcaatgc cagaatttcc tggctatact ggggatgcta
     3841 atccagctgt acaagtagtg aaagtcaaag cagttactca ccgaaaagac aatccaatca
     3901 tgcaaacaac gattggtccc agtgaagaac acgtatctat ggccggtatt cctacagaag
     3961 caagtattct tgccttggtc gataaagcta ttccaggaaa agtagtaaat gtctacaatc
     4021 ctcctgcagg tggtggtaaa ttgatgacca tcatgcaaat ccgtaaagaa aacgaagcgg
     4081 atgaaggtat tcagcgtcaa gcagctattc ttgctttatc agccttcaaa gaattgaaga
     4141 cagtcatttt agtcgatgaa gatgttgata ttttcgacat gaacgatgta gtttggactt
     4201 tcaacactcg tttccaagga gataaagata tcatcgtgat cccaggaatg aggaaccatc
     4261 cattggaccc atcagaatta ccagaatata atcctggctc tattcgtttc cgtgggatga
     4321 gtgcaaaaac tatctttgac ggtacagttc ctttcgatat gaaagaacat tttgtacgtg
     4381 caaaattcca agaagtagaa gattgggaaa aatatttgaa ataatgaatg atctgcaact
     4441 gttttgacat cataaaaagg agtgcggaaa atggaacaat tggcacaacg gaagaaaaaa
     4501 atcgtgattg gtgtaagtgg cgcttcaggc accatttatg ccatcaacct agtgaagaaa
     4561 ctaaaagaat atccgatgat agaaacacac ggaatcatca gtccatgggc aaaacagaac
     4621 ttaaagctgg aatccgactt atcactaact gaattcgaaa gcctgtttga ttaccattat
     4681 tcaaacaaag atttaggtgc tgctgtcgcc agcggatcat ttttgaccga tggaatgatc
     4741 atcgtaccgg caagtatgaa aacagtcgca ggtatctctg ttggtttagg cgacaacctg
     4801 attgcacgtg ctgctgatgt aacaatgaag gagcaacgaa agttagtcat tgttcctcgt
     4861 gaaacgccat taaatgccat tcacttggaa aacttgacta aattagcacg actaggtgtg
     4921 caaatcatac caccaattcc tgctttttat aatcagcctc aaacgatcca agatcttgtt
     4981 aatcaccata caatgaaact tttggatcaa tttggaattg taaatgagca ttcaaaacgt
     5041 tggcagggtg attaatatgg aaaaagaaac gatagttgaa gtaaccaaac taggtttcac
     5101 gatccaagct gctgttaaag taatcggtaa agacttattg atcactttaa caggcggaga
     5161 tacaccacat atcggaacag tcactacatt ttctcgtgat acagagatgc aaactgtacg
     5221 tttccctagc cacgatggtc ggttccataa agacgatgtc ttagccgaaa aaatagctgt
     5281 gatcatcaaa actgccgttc cgggaagttg tacgatcact tccggtatcc atgttgacca
     5341 tatctccaat gcacaaatca acgcctcttt tccgatgtca gaagagttag gttacaagat
     5401 tttagattgg ttggaaaaaa tagatttcca tatcaaagaa ccagtttatt ataaagatgg
     5461 tgaaaaaaca ttatgaaaga agaggttgtg aaatcagcgt tatgacctga gtatgagaga
     5521 aaaaaatgga aaaatagctt ctcatatttt gccgtttttg agctaatacc gagggacagc
     5581 tgattgaacg ccgtttatca agaggttgtg ataaagccgc ttcgtttcga gtagtaagaa
     5641 aaattttgta aacatgggtt tccacatttt tgcataaatt tgacttacta ccgagaaact
     5701 ggcttttgaa caccgtttat caagaggttg tgacacgtct tttgtcacaa cctcttttat
     5761 ttttgcttca ctgtaactgc tacaatatca ccgatttctt ttccgatgtt tttacgaata
     5821 tcttttcgta gtccaaggat gtagcagact gaaccatctg gatttttcac gcccatattc
     5881 acgatactgc cttcatacgg catattgtca aaagttgctt ggactttaac tcttccagca
     5941 ccgaattctt ttcttatatc atacggaaaa ataatatatg ccccgccctt gtcgacttcg
     6001 gaagcataga tttttgattc aaaagaataa atttccctca ttttatttct cctatttttt
     6061 acatctagta tagcatagca aaaaaaagaa ggaactgtga caaaagtctt gtcgcagttc
     6121 cttttttcga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcaa
     6181 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct
     6241 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct acggcactaa gcccaatcag
     6301 atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg cagagcagct
     6361 ttttcacaac ctcctacatg gtgttcaaaa tattattcgc cttttgcttc tttcactaat
     6421 tttgcgaatg cttgttgttg gaattcagca gctaaggctt tcgtacgttg ttcttgctct
     6481 gctttgctca tttttggatt ttcagtcatt gcttttacca taccgtcttg aacataagct
     6541 tttgcttcac tttccatcgt actttgctgt ttcttcacta attcagctaa cttggctgct
     6601 tggtccgtac ttaattctac ccatgtctct gggattttga atgtattcac tcggctgtca
     6661 tattcgtgat tgttatcagc aatattgaac cagaatttat acatgtcgtt tttgtattcc
     6721 caataagtag ttttcgtcac tttttcagca tcgccgtctg tcttttctac tttatttgtt
     6781 tcgttatccg taccagttgc ttttggtttt tcttgttttt catttgttcg gtaaagatac
     6841 actttttctt ttccattccc taaagcttta tacaaaagca tatctgctcc ttgggaatca
     6901 gcactggaaa caattttttc tgtttttgtt tccgttactt tttccattcc aaagtgattg
     6961 gacaaattag cgacgatcaa acttagtgaa gcgacgaaga ttagtccgaa aatcaaagat
     7021 aaaaaggttt gccatgtttt tttagcgaac acgttcgtta aagcaaaagc aagcaagctg
     7081 acaatcaaaa ctccaataat catgcattat cccccgcttt cattagaaca ggttcttttt
     7141 tcttcaataa aaaggcaata ataaagccaa gaatcccaaa gatgatcgct acgataaacg
     7201 cggcatggta acctgacagt gtggcattga ttgcctgatc tttataagct aatggcgtat
     7261 tatccaatac agatttgctt gggatatttg cttttgtcac attcgtcaac acactgatca
     7321 aaatagctgt accaatcgag cttgcgattt gacggaatgt attatttacc gctgtcccat
     7381 gactgatcaa gttatttggc aatgcattca taccagaagt cgttaccggc atcatgacca
     7441 tggagatccc aaacatacgg atcgcataaa ggatcacgat atataaaact ggagtcgttt
     7501 ttgtcaagaa agcaaatggt gcagttgccg ctgtcaaaat gaacatccct gtgatggcta
     7561 aacgtttcgc gccgtattta tcgaaaatcg cacccgtcac aggcatcata gcccccatga
     7621 tcaatgcacc cggcaacaac atcaagccag aatggaatgc tgattctcca cgaatatttt
     7681 gaatatacaa tggtacgacc atttctgccc ctaccatcgc catatttgtt acaccagcta
     7741 aaacagctgc aatcgtgaat gtagatgatt tgaatacacg aagttccaag aatggatgtt
     7801 ccaaatgtaa ttgacgccat gagaacaagg cgatgaaaat cacaccaaca actaagaaac
     7861 caattacttt caagcttccc cagccgtcat ctcctacact agaaaatccg taaagtaaac
     7921 taccgaatcc aatcgttgat aaaatagcgg ataaaatatc aatgctagga tttgacaatg
     7981 gaataacact tttcattagg aagaatgcta aaaccaagac aagaacaacg attggcagta
     8041 ccataccaaa caagtcacgc caatggtagt tatcaatgat ataaccagat aatgtagggc
     8101 ctaaagcagg agcaagtcca ataacgatcc ctgacatccc cattgctgat cctctttttt
     8161 ctggcgggaa aatagatagc atgatatttt gcaataatgg catcgatact cccacgccgc
     8221 aagcttggat caagcgaccg attagtaatg tactaaaatt aggtgctgta aagcaagtaa
     8281 tcgttccgat caaaaagatc gtcatggcag aaagatataa atttcttgaa ctgaatttat
     8341 tgatcagcca tgctgttatt gggatcatga tcccgttgac tagcaagaaa ccagtcgtta
     8401 accattgcac atcagacgct gaaatatcga aagctttcat caatgtagga aaagctgtcg
     8461 tcaataaggt ctgattcaaa acggtacaaa atgtaccaat taataagaca acaactaaaa
     8521 gtgtccgatt atatggtttt ccgtaaatat ctacgggttg gttgttactc atatttctcc
     8581 ttctttctga attttttctg aaactactag aatactttag tcctaaattc gctatttgtc
     8641 aacctacttg acacataagt taatacggat ataattaagg gaagcaagag gacaaaagga
     8701 gtgacaaaaa tggttggggc ttcccttaaa cgtttgttgg aaaatgatca attggttgtt
     8761 ggcatcagtg aattaagtga tatcgtaggt gtgtctccac gtcaattaag gtattgggaa
     8821 caaaagggat tcattcgttc gatcgaagct gatgcgaatt gtcctagaaa atatcgttta
     8881 cctacagtca taaaagtaga actgatcaaa aaatatttag atgacggatt tactttgtca
     8941 aaagctgcag aaaaggcaga aatccgccaa aagaaaatgc accatgtccg taaagttttt
     9001 tcaaaagcaa tcaaagatat cgaattgatc gacgaacgct atacgatttt acaaatcggt
     9061 aacttcaaag aagagaatgc taagatgtac atcattcatg acaacgagac agacgaatta
     9121 acttacaagc tccttcctgt cgatgaaaaa gctgactacg aacagttgtt aaaagaactc
     9181 taaacgccac taatctccta agtaccaagc tttttaataa gtagatgcta cataaatact
     9241 tgaaaaaagg cctgtttgaa cataagaata tccgaacggt gatctgcgtt tctttcatat
     9301 agaaaggaat tctccatcac tgttcggata tttttttatt tttttcggtt actttcttct
     9361 aatacatctg ttagccaatt tgcatattct gtttcccgat tgatcgcatg cgctaaaatc
     9421 aggtagtgtc cgtaattttc atgtttttct ttttcagtag gaaagatcgt tttttttcgc
     9481 tctaatagat gaagtcgttt ttcttcatga aggtcacgct gctgctgaat aatttctact
     9541 aatgccggat cattcacgtc tttgacgaaa tacaatttca gaacaaattc gtctcgatta
     9601 accggtagtt cattggttgg tgtatgaatc cactctgcca attgttcctt cccttcttct
     9661 gttaattcat aaactttctt ttccaatttt tcacctgtga tctctacttg atgctgaatg
     9721 attccttgct cttccatttt tgcaagttca ggataaatct gactatgttt tgcttgccaa
     9781 aattctccta tttcattttt gaacgattga ttcaattcat atcctgtcat cttcttctta
     9841 cttaataacc ctaacaaaat atatcgaagt gtattttttc ttgccatcag ttttccctcc
     9901 ttgacaaatc tttaaatcca ttctatcata aatgagtaat cgaaaacatg tagtttatta
     9961 catgttttaa tttataagga gatgcttaaa catgaaagaa tttaaagaat tggaagattt
    10021 tattggaact cactttatct atacgtatga caatggttgg gaatatgaat ggtatgccaa
    10081 aaatgaccat actgttgatt accgtatcca tggcggaatg gtcaaaggcc gttgggtaaa
    10141 agaccaagaa gctgatatcg ttaaattgac ggacggcgta ttcaaaatca cttggacaga
    10201 gccaacaggg acagacgtgg cattggactt tatgccaaat gaaaagaaac ttcacggaat
    10261 gatctttttc cctaaatggg ttgaagaaca tccagaaatc actgttactt tccaaaacga
    10321 acatatcgca gaaatggaag cagctcgtga aaaatatgaa acttatccaa aattacttgt
    10381 tcctgaattt gctacgatca cttatatggg tgatgcaggt atagacaatg aagatgttat
    10441 tagcgaagaa ccatacgatg gaatgccaga tgatatccgt agcggaaaat acttcgatga
    10501 aaattataaa cgagtaaaat aaggttgtga aagagaggtt gtgaaatcag cgttctgacc
    10561 tgagtattag agaaagaaac ggaaaaatag ttcctcatgt tttgccgttt tttgagctaa
    10621 taccgagggt cagctgattg aacaccgttt atcaagaggt tgtgaaatca gcgttatgct
    10681 ttgagtaata agaacgctag ttagaaaata gcttttcata ttttctaggc tagtgggctt
    10741 attaccgaga agcagccgtt tgaacaccgt ttatcaagag gttgtgaaat cagcgttttg
    10801 acctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
    10861 taataccgag ggtcagctga ttgaacaccg tttatcaaag attgtaacaa ctgcatattc
    10921 acacagttaa attagtgact tcaaaatgaa ataatgctcc cagcaaaaaa ttaggctggg
    10981 agcattattt cgttattctg ttcatcaaaa atcagcgttt tgattccatt tcatcacaag
    11041 ttcctcctca tttactgctt catctttttg ctgtgtttga attgtaaaac cttgtgaaag
    11101 atagaaatga atagctgatt gatttttttg atagactgtc aaagtcagtg ttttattggt
    11161 ttcttttatt tttttcatta gttgcgtacc gattctttgt tgccggtatt cccttttcac
    11221 aaagattcct gcgataaact gatcaatcac cccagcaaaa ccaataattt cttgatctat
    11281 ttctgctaag taaatcactg catctggtaa agctttccta acagattcta cttgattctc
    11341 ccaataagtt gatggaatga attcatgaga atccaagtta gcatgcagcc aaatcgatag
    11401 aacagattcc agttctttgg cagacaattt tgttgttgtg cggatcattt tttctctcct
    11461 tacttattca ttgattttaa aataaaaacc atttttttga agctctttta caagttttcg
    11521 cccctcaaaa aagtatatct tctcttgttt tacttttcta ataccaaaag aaacagcaac
    11581 tttaggatca tcagctgaaa cgattcgata agttccacca ggcaattgat ccagcttgac
    11641 tttcccatga tggtcgcttt gtactgaaaa cggcacacca tttctagtaa gtgttttttt
    11701 cttatgtttc aaaatgaatg aactaccatt taccggtttc cctgacgaat cactaataat
    11761 aaatgaaaaa tcaagcactt ttttacttaa tctatactga tagattagtc gtccgataaa
    11821 cactaacagt atgactacag ctattatcgt tcctgcaaga ataaaccatt gccgcatggt
    11881 tctttcctga tttccttttc cttcttcttt ttttaccgat tcgttataag gaatccgatg
    11941 tcctgtcact agcaaacgat gcgtgttgat catatacggc gtacatgtaa gcaaggtaac
    12001 taagtcttct ccttctgaaa tcgttagaac agacgtgtca tctggcagta ccacttgtat
    12061 atttttaact tgataagcca atttctcccc caaaacagtc aaaataaacg tgtctccgtt
    12121 ttctaaacga tctaattcgg taaataaccg tcgattaggt aaaccgctgt gagcagagat
    12181 cactgaatgc gtcgaatttc caccaagcgg gaaagaggtt ccttgcaaga cgcctgctcc
    12241 attttcaagg atcctaccat tgagccgatc ataaagtgaa atatttaatt ggatcttagg
    12301 tatattgata cttccgatca gccattcctc taattggctg gatgttgtac tttcatttct
    12361 gttcttttca ttgaaaggat cagtaccagg attgaatcct tggtcagtta atcgttgttt
    12421 tttctcctcc attttctttc gctgcacttc aatttcagct gcggtccgtt tttgtacttg
    12481 ttcgatacgt ttttgatcaa taaaactatt taaagaatcc acataaaatg gatataaagc
    12541 gatcagtaac cctacgacga tacatattaa taaaaacaaa cgaaacaaaa gtcgacgaga
    12601 ctgtttttgc ttactctgct tcacatgtgt cctcccttgc aagttttttt gttctttctt
    12661 ttattatatg ggttcaggaa aaaaatagaa aatgaaagcc gatgaggttg tgaaaaagct
    12721 gtcctgcatc aagtaataag tacagcccaa aaaaatagct actcatattt tttgttgggt
    12781 tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg ttgtgaaaac
    12841 tgcgttatgc tttgagtaat aagagcgcta gtcagaaaac agcttctcat attttctagg
    12901 ctagtgagct tattaccgag aagcagctga ttgaacaccg tttaccgaga ggtcatgaaa
    12961 aagctacttt gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag atgcaacaaa
    13021 acttatgtcg catcctctct ccttagaact ttcacttatg tttttcttgt aaaattcata
    13081 aatattttca tgaattttct aatttcttgc ctacctctcg cataggatat atgatgaatt
    13141 tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata tggcccattt ttagcaattt
    13201 ttttcattgc tttggcattt ctcatgcctc atttattcac acataaacta attttgggtg
    13261 ctgatgcccc attccattac aatcggttct atgatgccgc gcaacaaatt aaatatgggg
    13321 atttccatta ttttccttcc ctttatggat tccagcaaag tggcaggatc gtgaatgccg
    13381 tttacggccc atttttcgct tatttacaag gattgatcgt gttgcttgca ggtagctggt
    13441 ttcattatca gattctttca aatttcattc tttatcttat ttcaggattt tctatgctga
    13501 ttttactgag atatgtcaat gttcgaaatt gggtaagtgt accagtagcc gttttattca
    13561 tgagtacgta ttctattcaa tactggtcta tcaaccaagg attcactagc tgggggactt
    13621 gtttttatcc tttatgtatg atccctattg ctgatttcgt ccttcataaa aagttccctg
    13681 ttatcaaagt cgctgtctca gtgggactta tgacacagat ccatttactg tcttcgatca
    13741 tgttagcttt gatgtatatc cctttttatc tttattactt ttttaaccag actgaaaaag
    13801 gaaaagcact gcttcaactg attaagtcgg tcttgcttta ttttgttctg acagcaaata
    13861 tttgggcagg attagctatt gtttacagtg ggaataagat tcttcctccc tttgttaaca
    13921 gagaaatgag tcaaaaggcg atagatctgg atggcagtta ttggctccat taccctaagg
    13981 cctttcctat attgatatta tcaggttttt tgctttttat tttctttcaa caaaagaaca
    14041 ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt tctactagct
    14101 tgattccatg gacgacattg atcgaaaaaa atgtgccttt tattagtttg atccagtttc
    14161 catttcgatt tttcgtgcca tttacgctat tgcttctact ttaccttgcc ctttcactga
    14221 accagtggtc tgaacaaaag tggttccgtc ttttaagtat atttggcatt cttatttttt
    14281 cagcacagac gatccagaac ttataccagc atctggaaaa gtgggaaaat gaaacatttg
    14341 tcagtcgaca tacttatcta ttcgatacgc cggaagaagc aaggaaaaca ttcttcagca
    14401 aagatatgtc taccagcttg ttcgttttcc aaaaaaccac acctgactat ctgccaatct
    14461 ataaggagac aaaagacaat aaatacgacc ggtataacga agaaatacta gaaaacgaaa
    14521 caacttttat aaaaacacat ccaggcggtc ttttgacaat caagtggaaa aacactgggg
    14581 acaaaactgt tcatatccct gttatcgtct acgatcggac tgtgctacag caaaatggaa
    14641 aaacattgac tgattatgaa gtaacagata tcgggacccc aatcgtgaag cagaaaaaag
    14701 gaatcaacga gctcacgctt cattatcaaa cgccaatcta tttctatttc attctttctc
    14761 ttactttgat tggctggttt accttgctat gtttatttat ttatcataga tacaagcttt
    14821 tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa acggtataat
    14881 cgtttggaga gttttatttt aatcagtttt tttatgttat atgttatatt ttttattcgt
    14941 tcacatctaa ttcgatttcc accccgtagt gatcactgat gattggacta tcgatcccat
    15001 caaaaatccg ttgatatttt ttaacctgca ttccttcagg gacgaaaata aaatctatac
    15061 gcaatttttc tgtattttgt tcccaaccat ctattttctt ttccacagta gctgcttttc
    15121 ctttttcatt cgcgacaaca tacgaatctt gaagaagaag gcaactttct cttacagtat
    15181 aataaccttg agagtctact ggattattga aatcacctag gaaaaaaagt ggtacgcgag
    15241 tttccaaaaa gtagttttcc aagtttttcc actcaaaata aaagccactt tttttatctg
    15301 tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg atcatctatc tttgttttcc
    15361 caatcaagat cttgcgtgtc tgataatctt ctttcttttg agattgggag acggtaagga
    15421 cctgactttc caatgttttt ttcgtaagca gtgcatttcc ttcttcatat ttatcataac
    15481 cgatgtggct catttcccaa ctccaaaaat actcttttcc gtgttccttt aaaagctgaa
    15541 ctaaacggta ggcaaaatta tctttacgga taggtacttg attctttacc tgacagaact
    15601 tcattagctt cgcaggttct acttcttcag attccagtaa ttggttgatt tcttgtaaag
    15661 caatcacttc ataatcttct aacaggatct tatctgctaa ttcttgcaat tttttctctg
    15721 gttctggctc aagccagcta tgcgtgttga gtgtcatgaa tttcattggt tttctccttg
    15781 tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt cacaacctct ccagcaaata
    15841 gtgttctcct gctaactcct cagtaataag ctcaatggac caggagctat tttttgtttg
    15901 gctgttttta tcacttggtg cagggcagct ttttcacgac ctctccacta aacagtattc
    15961 aaactgctgc ttctcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt
    16021 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctcct tacattttaa
    16081 tggagccgat cactttacca gctgtcactg tacctaattg cttgatcact acttcttcta
    16141 ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt tggatcacat
    16201 caagatccat ctcagctaat ttcgttcctg ttcctacttc ttggccttct ttgacagcaa
    16261 tctcaaaagc tggctgattc atttggactg tatcgattcc catatggatc aaaacttcga
    16321 taccatcttt cgtttccagc ccaattgcat gtttgctagg gaaaatactt gtcacttttc
    16381 cagcgatcgg cgcaacaact tctcttgatg ttggaataac agcaaagccg tcacccatca
    16441 ttttttgtga gaataccgga tcttttactt cagataaagg aatcacttct ccagaagctg
    16501 cggaaactag ttcttgttct atacctaagt attgcttact acctgcttgt ccatttcctg
    16561 tagaaaccat ttctggctct ggaatctctg ctcctgattg cagcagatct tcgatatctg
    16621 atttcaagac atctgctttt ggtccataaa ctgcttggac tccattgtct ttgacgatca
    16681 atcccatcgc ccctgctcgt ttccaggctt cttcagtccc tactttttct cgatcattta
    16741 cacttacgcg taaccgagtc atacaagcat cgacatcttt gatattttct tttcctccca
    16801 gcaaatgaat gatctggacc acttgctgat ctgctgttcc tgttccgctg ttagatacaa
    16861 cggtttcttc actgtcattg tcgtaatttc cgttacgtcc cggtgtagca aagttgaatt
    16921 ttttgatcaa gaaatttgct aagaaatacg taacaacacc aaaagcgatc acacacaaca
    16981 cgaagttgat aagatctcca cctagacccg ctttgatagc taacggtgtc cgagtcagca
    17041 attcgatatt cccaaatgaa tggacccgca atggcaagat atctgccatc gcaaatgcag
    17101 ccccttggat cactgaataa atgacatata atggcacagc agcaaacata aacatgaact
    17161 ccaatggttc tgttacacct gttagaaaga cagccaacgc tgctgaaaaa tacattgatt
    17221 tatatcttgc tttcttatca ggatcgacat tacgatacat cgcaaaagcc attcccatca
    17281 agatacctga agaaccaatc atttgtccta ctttgaaacg agccggcgtc cagttatgca
    17341 acacaaactc atattgcgac atatcccctg cacctttaag attcactaaa tcagtggccc
    17401 aagccaacca taacggatct tgcccaaata cttgtgtccc cgcctgtgcg cctgataaaa
    17461 tctcatacga tccgccaagc tgtgtatagt tgataggaat cgtcagcata tgatgcaatc
    17521 caaatggcaa tagtaaacgt tctaatgttc caaataaaaa cggtgctaga atcggtgcgc
    17581 tttcttgtga ttctgcaatc cataaaccaa aattattgat tccagcctgt acgtttggcc
    17641 acaccacagc aagaatcaat gccacgattg ttgaccataa gataacaaca aacggaacaa
    17701 agcgctttcc attgaaaaat gacaaagcat ccggcaattt tctataatta tagtacttat
    17761 tataagccat tgccccgaca aatccagcga tgatccctac gaatactccc atattcaaag
    17821 ctggtgcttc caatacactt gtaaaaaatc ctttaaccat gatcttcgta ccgaacagag
    17881 tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag atagccccag
    17941 tgatccgatt gattaaaata aatgagatcc cagctgcaaa tgctccgcct gctctttctt
    18001 ttgcccaact tcctcctatt gctaaagcaa ataaaatatg aagattacca ataattgccc
    18061 aaccaatatt ttcaattact ccgcccgttg tgacaagcgg cgtccaatca ggattgatca
    18121 agggaattga tttccctata ctgatcatca acccagctgc cggcataact gctaccacaa
    18181 ccattaaagc ctttccaaat ttttgccaaa actcaaaact aaatatcttt ttcattccgt
    18241 ttagctcctt tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt tcttttttgt
    18301 aaatgcttta ttttttattt ttctgtctat accattctat aaaacctata atacgtgtgt
    18361 tcccttctat aattttctcc taaaaattta tttttttaag cttttttcta tgaaatattt
    18421 tcttttgtct attgacttgt gcaatcggtt gcgtttatta tgaacgtata aaacagctca
    18481 ttaggaggag aaaaatgaaa cagatcaaac gactatttgc tattgatcca tggaaaatca
    18541 gcacgacaca attagataaa aaaaatcttc gactgcaaga atctttaaca agtattggaa
    18601 acggttatat ggggatgcgc gggaactttg aagaaactta ctcaggggac caccatcaag
    18661 gtacttattt ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg tggaaaaatg
    18721 gttatccaga atactttgga aaagtgatca acgccttgaa ctttatcgcg atagatattt
    18781 cgatcaacga tcagctgatc gacttggctt ctttggaacc agaagacttt tactgggaat
    18841 tagatatgaa aaatggtgta ttatctcgta cattcacaat cactacttct actaataaag
    18901 tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct tatatctatt
    18961 taactataga aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa ttggatggaa
    19021 atgttcaaaa cgaagacagc aactatgatg aacatttttg gaatgagatt gatcgtggaa
    19081 ctatagaaga aatcggctat ttgacgacta aaactattcc aaacaacttt gggattgaac
    19141 aatttactgt tactgcagca atgcgccatt tcgttaatgg aaaatcagtc atccctgact
    19201 atcacgaaga tactttagca ttacatggtt gcttgtctgc agaattatct caaggtgaga
    19261 catggtcagt ggaaaaagaa attatcgttc ttactagtag agatattcca gaagcgcagc
    19321 aagcagaaaa agctgtctca gaattaacga accagacaaa caattatatc caagcaaaag
    19381 aagagcaaac agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc atcgaaggag
    19441 acgaagaagc acaacaaggt attcgattca atttgttcca actattctct acttactacg
    19501 gcgaagatga acgattgaat atcggcccca aaggattcac aggagaaaaa tacggcggag
    19561 ccacttactg ggatacagaa gcgtatgctg tccctcttta tcttgcttta gccaaacctg
    19621 aagtaactaa gaatctgctg aaatatcggc ataatcaact gccgcaagca atccacaacg
    19681 ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt caccttcact ggtgtcgaat
    19741 gccataacga gtgggaaatc acattcgagg agattcaccg aaatggcgcg attgcctatg
    19801 caatctacaa ttatacgaat tacaccggcg atacggaata tttgaaaaat gaaggactgg
    19861 aagttttagc agaaatcgca cgcttctggg cagaccgtgt ccatttttca aaacgagcaa
    19921 ataaatacat gatccacggt gtgaccggcc caaacgaata tgagaacaat atcaataaca
    19981 attggtatac aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa aattatttaa
    20041 cttatcaaaa agaagctgct atcactatca gcgaagaaga actagccaaa tggaaagata
    20101 tcgcagagaa catgtacttc ccagttgacg aagaattagg aatctttgtc cagcatgaca
    20161 cattcttaga taaagatctc atgcctgtat ccgatattcc cacaagcgat cttcctctga
    20221 atcagcattg gtcttgggac aaaatcctta gatcttgctt catcaagcag gcagatgtac
    20281 tacaggggat ttatttcttc aatgatactt ttacaaaaga agaaaagcaa aaaaactttg
    20341 atttctatga accaatgact gtccatgagt cttccctttc gccaagtatc cattcgattt
    20401 tagcagcaga attaggaatg gaaagcaaag ctgtagaaat gtaccagcga acagctcgct
    20461 tggatttgga taattacaat aacgacacag aagacggctt gcacatcact tccatgactg
    20521 gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa aactgcccat gatcagttga
    20581 gattcgcacc atttctgcca aaaacttgga aagcttattc tttccatatc aattatcgcg
    20641 gacgtttatt gttcatctca gtcacagatg aacaagttac tttcgagtta ttagaaggtt
    20701 ttcctctaga aatttacgtc tatgaacagt cttattcttt agcggatcgt ctagaaataa
    20761 cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt ttgatttaga cggtgtcatt
    20821 accgatactg ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga aatcggcatt
    20881 acgatcgacc acacattcaa cgaacagcta aaaggcgtca gccgagaaga ctcactctct
    20941 ttgcttttag cttatggcgg aaaagaacac tctttttcaa aggaagaatt tgcagaatta
    21001 gcgaaaagaa aaaacgatta ttatttagag atgatccaga cgatcgaacc aaaagatgtc
    21061 ttccccgggg tcgtgccttt actggattct ttaaaagaag aaaaaatcaa gattgctctt
    21121 gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac accttacttt
    21181 gatgctattg ccgacccggc agaagcaaca aacggaaaac ctgcgccaga tatcttcctc
    21241 cttgcagcaa aagctgtcgg actaaaagca gaagaatgta tcgggatcga agacgcgcaa
    21301 gccggtatcc aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg acgtgctgaa
    21361 gaccttggag aggagatccc gcttgtccct gatacgactt ttcttacgat cgattacttg
    21421 aaagaaaagt gtcatgacca tggataatcc agagatctcc gtcgtaccag ttccagagac
    21481 tgaaataaag aaaatcacat tgaagaacga ctacttagaa gtcgttcttc tcaattacgg
    21541 tgcccgcctg caccaaatct tcgcaccaga caaagaagga aaatcagaaa atgttttatt
    21601 gtcttacgat cgtttcgaag atgttctaac agacaaaagc ttttttggag caactgtcgg
    21661 acctgtagcc ggaaggatcc gggatgcttc ttggggtgag catagattgg agaaaaactg
    21721 cggatcacac catatccacg gtggcacgaa aggctggtct ttccaatttt gggaagtaga
    21781 agttttcaaa actccatcat caataggtgt ggtcttttac ttaaaagatg aattttctac
    21841 ctatccagga ccaatcactg ctacgatcac ttaccgttta acaaaaaacg aattagagat
    21901 gacaacctct gctagtagcc ttgttgaaac catctgcaat ccgaccaacc atgcctactt
    21961 caacctttca ggaaatggaa caagagacat ctatgaacat cagttaacgg tctttttaga
    22021 tggtgtgctt gaattagatc aagaaaaact gcctactgga aattggacaa aaaaagaaaa
    22081 actgccaatc gactttagaa aatccccaac actgcaagag atccttgcat gttatccaga
    22141 tggactagat gatgtatttc ttttgcatca tcctaggctt tccaaaactt ccttacaatt
    22201 attcgagaag cattctggtc gccaaatgac tatagcaact tctaataaaa gtatggtatt
    22261 attttctacg actggattcg aagctgattt ctctgtcaat ggaaaacaga tgcattcaaa
    22321 ctacggatta gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc ccaaatttgg
    22381 atcgatcaac ctccatcctg gtcaagaacg gatcagtcag acgatttatc ggttctctgc
    22441 acaataacaa gagaaaggtg tttttatcat ggctattacc gtaaaagatg tggcgaaaaa
    22501 agccggtgtt gccacctcga ccgtctctcg tgtcatcaac gatcatccta gtatttccga
    22561 gacaacaaag aagaaagtcc gaaaaattat ggatgaacta ggttatgttc ctaacatgac
    22621 tgcccgcaat ctaggaaaac gcatttccag tgccatcggc gtaatcttac cccctcttga
    22681 ttcaaaagag cggatcggta acccatttta tcttgagatc atggaagcaa tcaacgaaga
    22741 agcgcggaat tatgatatga ctacagcaat cgctactgct aaaaattttg aattgctgtt
    22801 agagaacgtc cagcgtatgc atttacaaaa acaagtagat ggttttattc tagtgtattc
    22861 agatagtcaa gatcctgtca ttgattattt ataccaaaac cacatccctt tcactttgat
    22921 cggacagcct tatcatcatg agaacgaaat cgtttatgta gataatgata accagcttct
    22981 aggtaaacaa gcaacagaat ttttgatcca gaatggacat aagaatatcc tgtttgtgac
    23041 aaacacgact catgaaagtt tgtatttcga acgatatttc ggttatcaaa aagcaatgat
    23101 gatggctggt cttcctgcgt tatccagtgt agtactagag catccggagg attatctgtc
    23161 ctttgagaaa acgttgaaag aaaacgaagc cacagcattg gtggtcattg acgatatctt
    23221 cgcccttcgc acgatgcagt tggcgcaaat gtacggctat gtcattccag agacactctc
    23281 cgtcattagc ttcaacaact cgatcttctc aacattaaca cacccttacc ttacaagtat
    23341 cgatatcgat atctccgaat taggacgtgt cgctatgcaa aagctgcgcg aattgatcca
    23401 tgaagaattg agcagtggcg tacgtttggt catccctcat cgcttgatca aaagagagac
    23461 agtcgttcct ttagaaaacc catactaaat agctgactca ctaaatacgc tgaccattta
    23521 tcctatggtc agctttttat gtaaaagaag atactacttc taacacattg tttatctgta
    23581 aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
    23641 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc
    23701 aattttcttg agtttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
    23761 tccttccttc aattttttac acgtcgacga ttcaataaaa aaccgcctaa tattgtaaag
    23821 acaggtgcaa gaatcgagaa aaagacgaat ggcaagtaat gcaacgcgct aactttcatc
    23881 gttcccataa tgaatgtgcc gctgactccc cacggtacga gagaattgac ggtcgcacct
    23941 gaatccgcta gtgttcgtgt caaataggtt tttgataagc caacgtcatc aaaagaacgc
    24001 ttgaatgttt ctcctggaag aatgatggac agatactgtt ctccaacgat cagattgatt
    24061 ccgatacagc tcaaggcagt aaacccgact aaacgagaag gtcggtccat cttttctttt
    24121 aattcttgaa cgatggtatc aacgatcaga tatttgatca gtaacccacc taatgccaat
    24181 gcaagtaaaa tcaaactagc agaacctaac atactcatga tcccgcctcg agaaagcagt
    24241 tcatctagct ttggatcagg tgtatttgcg acataaccat tcatcaagat gtctgctgct
    24301 tttccgaccg tcaaactttt ctcatggata aaagaaagaa cgagagcagc tgtcgatccc
    24361 gctagtaatg tagggatagc cggaattttt ttgattgccc ccccaaataa aataactagt
    24421 ggaatcaatg tccaaggaga gatccaaaag ctcgctttca tgttttggat catttcattg
    24481 atactagcta gatcggctga atgactgtta tgtcctaata tcgtataaaa aatcaacgaa
    24541 aggacgaatg ctgggaaatc cgtataacgc ataccagcga tatgttcgaa tagattaact
    24601 cctccaatac ccgctgccaa gttggtcgta tctgataaag gcgagcagtt attccctaaa
    24661 aaggcaccgg aaacgactgc gcctgtagtc attgcatcat tgaatccaag taaatgaccg
    24721 atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact
    24781 ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta
    24841 aatccataaa ccataatcgt agggatggtt ccagataaaa tccatgaagc aactaagaca
    24901 ccaatcaaaa gaaaaatcag gattggaatg atccctggag tgattccttt gatgatcccg
    24961 tcatggatct catcccatgt gaacttttta aatctgccat aaaacaacaa acacatgaaa
    25021 gctgcaagga tcgggatatg tggcgagagc tggaatccga tgatcagtac tcctaggagt
    25081 gccaacaaca cgatcaatac agcgatactt tcttttaaag aaatattttt attcatcgtc
    25141 gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt ggcaggacct
    25201 cctactaaat cagcgacaaa acgtcggctg aaagacttat tttttttatt tttcttctaa
    25261 acttttttct acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat
    25321 aacgaacatg accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc
    25381 taatgtagca aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc
    25441 taatccgttt ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg
    25501 cattttaagc aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc
    25561 atttgtaagt gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa
    25621 taatgaaata acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc
    25681 tttatggaat ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt
    25741 tgatggtcca gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct
    25801 gaagatattt ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg
    25861 cgtgatattt gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt
    25921 gatgacattc aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt
    25981 tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa
    26041 tgtgatacca gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga
    26101 ccatgaaaag tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac
    26161 acctaaaacg aacatgatga ctactgggat atatacccct agccatacac cgatgttccc
    26221 gccgactttt gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa
    26281 aatacaacct aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc
    26341 taacaatggt cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa
    26401 tagccatgca acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg
    26461 accgccttct tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc
    26521 aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt
    26581 aggaatactc cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc
    26641 cattttactt acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat
    26701 aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg
    26761 attctttgag tctattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg
    26821 aagagcttag gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt
    26881 caagctgttt tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct
    26941 gcgtagattt tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca
    27001 aagtgttctt ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt
    27061 ccggcacggt tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg
    27121 ttgccatagt ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg
    27181 cttcctttca cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact
    27241 aagtcatcgt tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt
    27301 aatggatgaa cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg
    27361 tagaaacgat gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc
    27421 aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct
    27481 tccaagatat aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc
    27541 gcaaagtaag agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa
    27601 tatggaacat agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct
    27661 ttatatgcac tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat
    27721 ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa
    27781 atcgcacggc cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct
    27841 ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct
    27901 gttgatccta caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga
    27961 acgatttttt caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc
    28021 acttggtcta aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt
    28081 tgtggaacca accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct
    28141 ttgatttcgt cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac
    28201 aagttcatta attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt
    28261 gcaagcggca atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa
    28321 ccatcagcaa cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt
    28381 cctacttctt cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca
    28441 ttccatagca tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata
    28501 tgaccccagt aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa
    28561 atttctgata gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt
    28621 tcttctggcg taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt
    28681 tcatccacca attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca
    28741 atgaataggg catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc
    28801 taaaaaaata tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac
    28861 taagttttgg cctgattttt aatcatattt taatatgtat caattatccg aattgttcac
    28921 acttttgaaa taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat
    28981 tgtattaacg acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat
    29041 agtatttccc tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa
    29101 agtaattttt cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat
    29161 ttatgacaaa atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa
    29221 catcttcacg tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga
    29281 tattttcagg tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt
    29341 cttccgcatt tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt
    29401 ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg
    29461 cttcacgttt ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag
    29521 acaagaaagt aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga
    29581 attggtagaa ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag
    29641 tttttccgaa tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt
    29701 ctacttctgg tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt
    29761 ggtctgctcc accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga
    29821 tagattgcag aatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg
    29881 caacgatatc ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa
    29941 attcaagtaa agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat
    30001 ctttaccaaa caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt
    30061 ccatcgtttg caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc
    30121 cgccgccaat cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa
    30181 atggaatcaa atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg
    30241 aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg
    30301 cgtcgcgcca agttaattca tcaataatat tcattttact gacctccgtg tagttttttt
    30361 atgaaagcag gaacctgcta gcatgtgtaa ttaattattt cttcaggata ggataggaaa
    30421 taaaaaaagt cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg
    30481 tatgtctaga tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc
    30541 caaagactcc tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc
    30601 tttctgaaac attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat
    30661 atgtgtctat catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa
    30721 attcctcatt ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt
    30781 gttaaaagaa ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt
    30841 tgcctcttat tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca
    30901 agggacgagg tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac
    30961 tatcgttatt ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc
    31021 aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca
    31081 actagttact caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt
    31141 attggaaaaa gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa
    31201 aaaggagaaa gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt
    31261 ccacagcttg atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa
    31321 agattatatg gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga
    31381 ttttgtttta gttaatttaa atgatttgat ccaaaaagac gaacaaggaa atctttcctt
    31441 taacgggctt gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag
    31501 ctattatgaa tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa
    31561 tgaaatcaaa aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt
    31621 tcctctgatc gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg
    31681 taccttggcc ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata
    31741 tggagacaat gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt
    31801 gaagaaagac ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc
    31861 ttcactagaa gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct
    31921 aggaaaaaca aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta
    31981 ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa
    32041 tgtcgatgct tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg
    32101 gatcaatatc gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga
    32161 tccattcatc aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta
    32221 cgtcatttat gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat
    32281 gctttatttc ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata
    32341 tggaagcagt tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata
    32401 gaggatcaag aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg
    32461 gaaaagatca agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa
    32521 ttcttttttt tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac
    32581 tcgattgaga taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag
    32641 attttccgca atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac
    32701 ttcataaatc agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg
    32761 agcgttctca gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa
    32821 gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt
    32881 catttgtaga gcctcccgta attttaaact tttataatat atatagttca aatttattct
    32941 gaggtttatt ataactatct attgagaaag tatcaagcta gcaattcctt gtttatgaat
    33001 tcattgacag aaatttataa aaagaatatt atgtactaaa ataataaaaa aagttttata
    33061 aaaaggtata aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa
    33121 aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg
    33181 tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt
    33241 tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa
    33301 aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat
    33361 ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg
    33421 agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga
    33481 actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa
    33541 tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga
    33601 tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa
    33661 attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg
    33721 actagcggat aaagtcgtga tcatacgcga aggaacagtc gtagaagaag gaacactcga
    33781 tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca
    33841 cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc
    33901 tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa
    33961 atttgaatct attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg
    34021 aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag
    34081 ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt
    34141 tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt
    34201 gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta
    34261 tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt
    34321 gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga
    34381 acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag
    34441 tattctgatc aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc
    34501 agattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat
    34561 tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa
    34621 tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc
    34681 tgattcctcc ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac
    34741 aactaacaac tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag
    34801 ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg
    34861 aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa
    34921 agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat
    34981 ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca
    35041 atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtcgtct tgatttgtct
    35101 agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg
    35161 tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt
    35221 actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc
    35281 tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg
    35341 gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg
    35401 gcttcctcga atgggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta
    35461 ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt
    35521 tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag
    35581 ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata
    35641 ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt
    35701 ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct
    35761 cgattctttc tttttacata accattctcc tctaataatt taaattttct agataaagtt
    35821 tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga
    35881 tccttccctg tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt
    35941 tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct
    36001 aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc
    36061 aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct
    36121 agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt
    36181 ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt
    36241 tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg
    36301 tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat
    36361 aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat
    36421 aacttccctt attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca
    36481 gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga
    36541 ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca
    36601 aaccttcact cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc
    36661 tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca
    36721 gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga
    36781 aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag
    36841 tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc
    36901 ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca
    36961 atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt
    37021 ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac
    37081 gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg
    37141 ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt
    37201 tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct
    37261 ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt
    37321 tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac
    37381 aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac
    37441 tgcgatgagc atcaccgcag ttttattgaa cttaaggttg attgatcaca tctaaaagcg
    37501 ttgaaaataa aatggcacta gaagcagctc ctgggtcgat atgtccgatt gcacgctctc
    37561 ctagatagga agctcgtcct tttttagcta ccaagacttt ggtgtgttct tttgcctggt
    37621 caatgatttc ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc agccaaacat
    37681 ctaacatcgt tttatcgtca gcttctgcct ttcctcggct ttgaataccc gctgttccct
    37741 gctcgatcac ttcgcctaat ttttcttgag aatcaataga atcgacctct tttgtcgctt
    37801 tcatcatgtt catgaatgca gatccgtata acggaccaga agccccaccg actttcgata
    37861 tcatcgccat agagaatact ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa
    37921 aagcttcttc ataagctgaa acccctcttc ccatgttatt tccatggtct ccatcgccaa
    37981 tcggggtatc tagatcactg agatattgtt tgttctgatt gattgcctct gcaaattgcg
    38041 tgagccattc ttggatttct tgaacggtta gtttcatatt atcacccttt tatcttgaga
    38101 ttttttacca tgaaatcgtc tgtacattgc tttctaaaga atctttccat tgtgtctccg
    38161 tcaagtcgat cagtgttaga gaaagacctt gcatatctag tgaggtcata aaattaccga
    38221 cttttcggaa aactacattg acgcctcttt cttccaacag agctaaaaca tcattcatga
    38281 acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca cctgcttctt
    38341 ttggtaaatt cgagtaatca tctaaaatct tagtaacaag ttcctttgcc agtactttag
    38401 aaggctgcat cttttctcga cgatagcctg gctcaccgtg gataccaaca ccatactcga
    38461 tctcatcttc tcctagttcg aatcctggtt tccctacttc tggtacagtt gctgctcgaa
    38521 gcgctagacc gatcgtctta gttgcctgta cgaccttttc tcccagttct ttcaactcag
    38581 ctagggaagc accgtttctt gctgcatcgc ccacgatttt gtgtacaagg acagtacctg
    38641 caactccgcg ttttccagca gtatacgtac tattttcaac ggcgatatca tcatctacga
    38701 tcacactttc tacttcaata tcgtccatag cagctaattc ctttgccata tcgaagttca
    38761 atgcatcccc tgtataattc ttaacgatca gcaaaactcc ttttccacta tccgcttcct
    38821 taatagccgt ctgaatctga tctggagttg gagaagtaaa cacatcacca agcaccgctg
    38881 cacttaacat cccttcccct acaaacccgg catgggatgg ttcatgtccg cttcctccac
    38941 cagaaaccaa accaacttgt tccgctttct gattcttggc tacaacacgg gaagattcta
    39001 cacgatgaac aagttctgga tagcttttta ctaatccttc cagcatttct tcgacaatat
    39061 tgcttggatc attcatgatt tttttcaatt tattcccatc ctttctttcc ggcattttgt
    39121 gcttctgtta agatttgctg agtatcatct gttataccgg cagtgatggc tgctgcaaaa
    39181 gctccttcca ctaaaggcgc atccaccaga tgataacgtt gctgctgctc ttcttccaac
    39241 atgtcttttg ccagttccga gttcaatacc gcgctaccga tatccgcaaa aacaaaataa
    39301 ctagctgcat cgcttgattc attgactgct tctacgattt tcatcgggtc tgatccgatc
    39361 tcgccattta ttccgccgag agaaaaaatt tgcacctctt cggatttttg catctgctca
    39421 atcatttctt taataccatc tgttactttt tgactatgag aaataagcaa aatacttttt
    39481 ttcacaagaa tcttcctttc ttttacatag ttgctaacga ttggtttcgc tttcattaat
    39541 gaacaattca agtatataat aaatttctaa ttttgggaag atatgtgtag tgcttcttga
    39601 agtttgttat actttaatta aaagagagga gtgatcgaat gattacaaca acaacgaaca
    39661 gtgtagaggg aaaagccgta gtagcttata aagggatcgt ttttggtgag gtcattactg
    39721 ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa
    39781 aaagctatga aaacgaacta atgggtgcaa gagaagaagc acttgccgaa atggccgaac
    39841 gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa aatggactat gaagtattag
    39901 gcagcgacaa tggcatgctg atggtcacat gtagtggaac agctgtctcc acgagataag
    39961 taggggacat ctgttttaat aactaaagag agatgggaca tgtagtggca ctcaagaatt
    40021 agatttttca gtctaacttc gaggtactct accttcccat cctctctttt ttatttcttt
    40081 ttgcggatga ttccgtaagc tgctgcgact aacacacccg cccctgctaa ggaataactg
    40141 acagaagctg ttttttcatt tgtttttggc aaagtctgac ttgttgattt ggatggctct
    40201 gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa
    40261 tgcaaaatat cttttattcg ttggaaacta gctgctaagt cagctttttt tgcttcaaaa
    40321 tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg
    40381 atatccgctt gagtttgact tagctttcca gaagctgttt gagcaacctg atttaattgc
    40441 tgattgaatt cttttgcttg ttggatcttc ccgtcaagat ctgtttggac agcttccgct
    40501 tttgctttcg cttcggcaag ctttttctca agttgtgcac ggtcatttcc cactttattt
    40561 ttaagatcag ctaaatcttg atcgattgct gcttggtctt ctttcaattt gttttttgca
    40621 gcttctaatg cttcttcgag ttcttttctt ttattttcca agtcttgcaa atgatcgatt
    40681 tggtcagaag aggtagaact atttattgat gtatcttctg aagatgaaat cgtttctcct
    40741 gaattatctg tcggtacatt ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca
    40801 gaagagctta tagttgttga aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct
    40861 gtctgtccca ataagaaaat actggaaaca gcaattggcc agattaacgg cttcattctt
    40921 tttttcatca cttttcccat ccttactact ttttaactaa tttcttatta taagcaaaaa
    40981 tgtttggaag aaccataaac acaacaggct gtttgtctaa aaaacggtgc atcttttatt
    41041 tttttgtacg ttccgggctt ttccatgtcc cgcttccata acctaaaatc gttttgactg
    41101 ctggaatgaa agtcgtcacg atcgttatcg cgttcactat ccagaacaaa agcatatata
    41161 gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc atctacaatc agcgaagcta
    41221 gcaactggaa aaatcctgcg accatttcaa agcagatgaa cacaaaactg aatgtaaaag
    41281 taatataaat ctgttcgtag tttccttgat atacataatg cccgattaat ccagcaaaaa
    41341 gaactacgga aatacaaaag aatattgacc agacaatact caatgtttga tcaatgaaga
    41401 tgatcgttct tccaatatgt ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc
    41461 agacttctgt tccacctttt gcccaacgtt ttctttgtcg atatagcatc ttcaacgtca
    41521 caggtacttc catgaaaaag ataatctcag gagcaaaaac agataaccag tcatttaact
    41581 gatgatccca agcaatactg atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat
    41641 cgatcaaggc ttcttttcga tacatcgtat ttgctccact gtacgcataa agaccaccga
    41701 aaacagctga ctgtgttcgt ttaatgatgc ccacgatact tgagaattca accgtttgag
    41761 attttgcaat caatttggta cgattttgga catccatatt tgcagtgacc gcagcaatat
    41821 gtcttgcacc agggcgaatg aaataattca catacctaat cagtgcatct ggctccggta
    41881 ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa
    41941 acgcgtgggc ttttccttta tttttttcga tccgaaccac acgaagatta gcgtattttt
    42001 tcatcagtct agctaatatt tctggtgttt ggtccgttga accatcgtct gtcactaata
    42061 cctcatagtt gtgataattc aatttcgtca tcaaatactc gattgtatct tcgatcacaa
    42121 tttcttcatt atgggctggc accatgatcg tgatgaacgg ttcgacactc aagggaacat
    42181 ctacccattc tttttgctta tgtttgtata aaaacacata acaccagaca ccaataaacc
    42241 atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa tacataatag acaaacaagt
    42301 atctaagaat aaattacctg tatgggtgat tgagatcatt tcagttccac cccattttct
    42361 ttatacaaat ctgcaatgaa tgtcgtatct agatttttct cttcggagac agaataaaaa
    42421 cggaccgttt ctctctcttc ttttgttcca aaacgttccg tatacacttc ttcaagtaat
    42481 tcttgtcttt ttttcaattt ttcatcttca tattcaaaag atttatttac ttttttgtat
    42541 agatttcgat tgttccacct agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc
    42601 aatacaaaaa agccgattaa gaaatacagc attttctttt ctgataaaaa agcgtacaag
    42661 gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa agacccatat aaaaggaaga
    42721 acaacgatca tccatcctaa aagagccact aacgtttgcc aaatctttaa ccagtaatta
    42781 ccttttgaaa aatacttatc tttcaccaaa attgatgcaa cttcttctct tgttactttt
    42841 ttcatatcct cacctcacgt
//
LOCUS       DACDKL010000007        41978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDKL010000007 DACDKL010000000
VERSION     DACDKL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.007"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IV329_000291"
     CDS             complement(38..1189)
                     /locus_tag="IV329_000291"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8939499.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IV329_000292"
     CDS             complement(1202..1639)
                     /locus_tag="IV329_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8939500.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IV329_000293"
     CDS             complement(1654..3420)
                     /locus_tag="IV329_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8939501.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IV329_000294"
     CDS             3684..4361
                     /locus_tag="IV329_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939502.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IV329_000295"
     CDS             4364..5491
                     /locus_tag="IV329_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939503.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IV329_000296"
     CDS             complement(5691..6131)
                     /locus_tag="IV329_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8939504.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IV329_000297"
     CDS             complement(6142..8175)
                     /locus_tag="IV329_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8939505.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IV329_000298"
     CDS             complement(8177..9880)
                     /locus_tag="IV329_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8939506.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV329_000299"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV329_000299"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8939507.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IV329_000300"
     CDS             complement(10963..11988)
                     /locus_tag="IV329_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8939508.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IV329_000301"
     CDS             complement(12197..13066)
                     /locus_tag="IV329_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8939509.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IV329_000302"
     CDS             complement(13525..15417)
                     /locus_tag="IV329_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939510.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IV329_000303"
     CDS             complement(15414..17141)
                     /locus_tag="IV329_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939511.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IV329_000304"
     CDS             complement(17157..17807)
                     /locus_tag="IV329_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8939512.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IV329_000305"
     CDS             18011..18307
                     /locus_tag="IV329_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8939513.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IV329_000306"
     CDS             18479..19099
                     /locus_tag="IV329_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8939514.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IV329_000307"
     CDS             complement(19191..20357)
                     /locus_tag="IV329_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8939515.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IV329_000308"
     CDS             complement(20369..20722)
                     /locus_tag="IV329_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8939516.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IV329_000309"
     CDS             20850..21872
                     /locus_tag="IV329_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8939517.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IV329_000310"
     CDS             complement(22311..22745)
                     /locus_tag="IV329_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939518.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IV329_000311"
     CDS             complement(22855..24714)
                     /locus_tag="IV329_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8939519.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IV329_000312"
     CDS             complement(24832..25992)
                     /locus_tag="IV329_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8939520.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV329_000313"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV329_000313"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8939521.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IV329_000314"
     CDS             complement(27739..28437)
                     /locus_tag="IV329_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8939522.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IV329_000315"
     CDS             complement(28394..30055)
                     /locus_tag="IV329_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8939523.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IV329_000316"
     CDS             30273..31673
                     /locus_tag="IV329_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8939524.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IV329_000317"
     CDS             complement(31925..33004)
                     /locus_tag="IV329_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8939525.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IV329_000318"
     CDS             complement(33090..34049)
                     /locus_tag="IV329_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8939526.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IV329_000319"
     CDS             complement(34061..35584)
                     /locus_tag="IV329_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8939527.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IV329_000320"
     CDS             complement(35594..36157)
                     /locus_tag="IV329_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8939528.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IV329_000321"
     CDS             complement(36163..37026)
                     /locus_tag="IV329_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8939529.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IV329_000322"
     CDS             complement(37040..37936)
                     /locus_tag="IV329_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939530.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IV329_000323"
     CDS             complement(38111..39382)
                     /locus_tag="IV329_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8939531.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IV329_000324"
     CDS             complement(39372..40889)
                     /locus_tag="IV329_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8939532.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="IV329_000325"
     CDS             41087..41977
                     /locus_tag="IV329_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8939533.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
BASE COUNT        12172 a         8757 c         7287 g        13762 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgat
//
LOCUS       DACDKL010000008        40037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDKL010000008 DACDKL010000000
VERSION     DACDKL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.008"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV329_000326"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV329_000326"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8939534.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV329_000327"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV329_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8939535.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV329_000328"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV329_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8939536.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV329_000329"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV329_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8939537.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV329_000330"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV329_000330"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8939538.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV329_000331"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV329_000331"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8939539.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV329_000332"
     CDS             complement(8053..8523)
                     /locus_tag="IV329_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8939540.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV329_000333"
     CDS             complement(8569..8994)
                     /locus_tag="IV329_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8939541.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV329_000334"
     CDS             complement(9061..9330)
                     /locus_tag="IV329_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8939542.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV329_000335"
     CDS             complement(9341..10936)
                     /locus_tag="IV329_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8939543.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV329_000336"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV329_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8939544.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV329_000337"
     CDS             complement(14489..15049)
                     /locus_tag="IV329_000337"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8939545.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV329_000338"
     CDS             15402..16376
                     /locus_tag="IV329_000338"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8939546.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV329_000339"
     CDS             16546..17946
                     /locus_tag="IV329_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8939547.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV329_000340"
     CDS             complement(18024..18746)
                     /locus_tag="IV329_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8939548.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV329_000341"
     CDS             complement(18825..19331)
                     /locus_tag="IV329_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8939549.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV329_000342"
     CDS             19454..21253
                     /locus_tag="IV329_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8939550.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV329_000343"
     CDS             complement(21321..22895)
                     /locus_tag="IV329_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939551.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV329_000344"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV329_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8939552.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV329_000345"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV329_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8939553.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IV329_000346"
     CDS             complement(24672..25310)
                     /locus_tag="IV329_000346"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8939554.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IV329_000347"
     CDS             complement(25311..25961)
                     /locus_tag="IV329_000347"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8939555.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV329_000348"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV329_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8939556.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV329_000349"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV329_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8939557.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IV329_000350"
     CDS             complement(29104..29844)
                     /locus_tag="IV329_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8939558.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV329_000351"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV329_000351"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8939559.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IV329_000352"
     CDS             complement(31215..32153)
                     /locus_tag="IV329_000352"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8939560.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV329_000353"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV329_000353"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8939561.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV329_000354"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV329_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8939562.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IV329_000355"
     CDS             complement(35313..35618)
                     /locus_tag="IV329_000355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8939563.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV329_000356"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV329_000356"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8939564.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IV329_000357"
     CDS             complement(36724..37008)
                     /locus_tag="IV329_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939565.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IV329_000358"
     CDS             complement(37026..37916)
                     /locus_tag="IV329_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8939566.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IV329_000359"
     CDS             complement(37936..38154)
                     /locus_tag="IV329_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939567.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IV329_000360"
     CDS             complement(38124..39824)
                     /locus_tag="IV329_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8939568.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT        10845 a         8714 c         7266 g        13212 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc tttttta
//
LOCUS       DACDKL010000009        37237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDKL010000009 DACDKL010000000
VERSION     DACDKL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.009"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..831
                     /locus_tag="IV329_000361"
     CDS             <1..831
                     /locus_tag="IV329_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8939569.1"
                     /translation="LVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVL
                     TKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTG
                     YELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANG
                     TVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLT
                     KENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI
                     "
     gene            complement(1255..1425)
                     /locus_tag="IV329_000362"
     CDS             complement(1255..1425)
                     /locus_tag="IV329_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8939570.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1619..1798)
                     /locus_tag="IV329_000363"
     CDS             complement(1619..1798)
                     /locus_tag="IV329_000363"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939571.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1808..1984)
                     /locus_tag="IV329_000364"
     CDS             complement(1808..1984)
                     /locus_tag="IV329_000364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939572.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2185..3297
                     /locus_tag="IV329_000365"
     CDS             2185..3297
                     /locus_tag="IV329_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8939573.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3375..3668)
                     /locus_tag="IV329_000366"
     CDS             complement(3375..3668)
                     /locus_tag="IV329_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8939574.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3836..4150
                     /locus_tag="IV329_000367"
     CDS             3836..4150
                     /locus_tag="IV329_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8939575.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4171..6150
                     /locus_tag="IV329_000368"
     CDS             4171..6150
                     /locus_tag="IV329_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8939576.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6163..6489
                     /locus_tag="IV329_000369"
     CDS             6163..6489
                     /locus_tag="IV329_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8939577.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6505..7023
                     /locus_tag="IV329_000370"
     CDS             6505..7023
                     /locus_tag="IV329_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939578.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7023..8375
                     /gene="celB"
                     /locus_tag="IV329_000371"
     CDS             7023..8375
                     /gene="celB"
                     /locus_tag="IV329_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8939579.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8459..9964)
                     /locus_tag="IV329_000372"
     CDS             complement(8459..9964)
                     /locus_tag="IV329_000372"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8939580.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10056..10646)
                     /locus_tag="IV329_000373"
     CDS             complement(10056..10646)
                     /locus_tag="IV329_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8939581.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10825..12471
                     /locus_tag="IV329_000374"
     CDS             10825..12471
                     /locus_tag="IV329_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8939582.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12520..13239
                     /locus_tag="IV329_000375"
     CDS             12520..13239
                     /locus_tag="IV329_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8939583.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13718..14665
                     /locus_tag="IV329_000376"
     CDS             13718..14665
                     /locus_tag="IV329_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939584.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14677..15597
                     /locus_tag="IV329_000377"
     CDS             14677..15597
                     /locus_tag="IV329_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8939585.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15638..17095
                     /locus_tag="IV329_000378"
     CDS             15638..17095
                     /locus_tag="IV329_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8939586.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17440..18054
                     /locus_tag="IV329_000379"
     CDS             17440..18054
                     /locus_tag="IV329_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8939587.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18047..19771
                     /locus_tag="IV329_000380"
     CDS             18047..19771
                     /locus_tag="IV329_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8939588.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19768..21219
                     /locus_tag="IV329_000381"
     CDS             19768..21219
                     /locus_tag="IV329_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8939589.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21692..23845)
                     /locus_tag="IV329_000382"
     CDS             complement(21692..23845)
                     /locus_tag="IV329_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8939590.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23847..24893)
                     /locus_tag="IV329_000383"
     CDS             complement(23847..24893)
                     /locus_tag="IV329_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8939591.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25043..26332
                     /locus_tag="IV329_000384"
     CDS             25043..26332
                     /locus_tag="IV329_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8939592.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26345..29047
                     /locus_tag="IV329_000385"
     CDS             26345..29047
                     /locus_tag="IV329_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8939593.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29032..29889
                     /locus_tag="IV329_000386"
     CDS             29032..29889
                     /locus_tag="IV329_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8939594.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29960..30817)
                     /locus_tag="IV329_000387"
     CDS             complement(29960..30817)
                     /locus_tag="IV329_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8939595.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31028..33199
                     /locus_tag="IV329_000388"
     CDS             31028..33199
                     /locus_tag="IV329_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8939596.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33267..33608
                     /locus_tag="IV329_000389"
     CDS             33267..33608
                     /locus_tag="IV329_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8939597.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33884..35638)
                     /locus_tag="IV329_000390"
     CDS             complement(33884..35638)
                     /locus_tag="IV329_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939598.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35649..36485)
                     /locus_tag="IV329_000391"
     CDS             complement(35649..36485)
                     /locus_tag="IV329_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8939599.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36487..37170)
                     /locus_tag="IV329_000392"
     CDS             complement(36487..37170)
                     /locus_tag="IV329_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8939600.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12013 a         6549 c         7638 g        11037 t
ORIGIN      
        1 ctggtagaaa ctcaagcgcc aacggggtat gaattagatg cgacaccgat cgagtttgag
       61 atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag aaaaccgact tacaccaggc
      121 ggtgtcgtgt tgacaaagat cgatgaccaa agcggggaag tcctgcaagg agctgttttt
      181 gagttacaag atgccaatgg aaccgttctg cagtcaggtc taaccacagg agcagatgga
      241 aaactggcga ttgacggctt agcaccagga gtgtatcagc tggtagaaac ccaagcgcca
      301 acggggtatg aattggatgc gacaccaatc gagtttgaga tcgaaagaag tcagacagcg
      361 gtggtagaat tgacgaaaga aaatcgactt acaccaggcg gtgtcgtgtt gacaaagatc
      421 gatgaccaaa gcggagaagt cctgcaagga gctgtttttg agttacaaga tgccaatgga
      481 accgttctgc agtcaggtct aaccacagga gcagatggaa aactggcgat agatggctta
      541 gcaccaggag catatcagct ggtagaaact caagcgccaa cggggtatga attggatgcg
      601 acaccagtaa ctttcaagat cgaaaaagaa caagaagtgg cgatcttttt aacaaaagag
      661 aatagaaaag ttgctgatag ctcagatata agaggaaaca atccaacaag cagtggaaat
      721 aagcatcttc caaaaactgg agaaaccatc attagccagt ttatattgtc aatattgggt
      781 gtgctacttg ttttcatcag tataaaattt agaaagaaac gaaatatatg agctaaaata
      841 aaaaattgaa taagcatagt ttttaaaatc aactagtacc catcaattga atggaaacta
      901 ccgattttag aaggggtata caaacaaata ttagtatcgg agtagagaag gttgaaccca
      961 accagtaaat aagtaaagac aattttgtgc tgcgttgaga agtgaaaaat aaacctttgt
     1021 agtaatacta gttaataaaa aatatacaaa aacactgcga aaatcattct tgagtttcgc
     1081 agtgtttttt gcctaatgac tcaagtttat tgggtaatac tttgattttc tttctacaaa
     1141 taatcagcga tcagttgtaa ttttttctct agttttaatg gctctttgtc aataaggaga
     1201 aaaacaagtc gggtcttttg tctcggaaag cttcaaccaa ctcgtgaata aaggttacgc
     1261 ctccttgaaa ggcgtggaaa aaccaagtaa tcgatcaatc accaatttcg catttggtag
     1321 aacatgtttg gtgagctgaa agtaggtggc gttcatgtct gtaactaaga attctatttc
     1381 tttaggatta gtgcagctaa ggaaatagtt tgttaatcga ggaatttttt gtgtaggtaa
     1441 attttctcga ttaatttttc tgtctcgcca tccgcggaaa taaaactcag tttatcctca
     1501 acggaaacgt gagaacgaaa ttcacaaccc tcaacactca agacagaatt cttttggaag
     1561 actttggtaa atagcttctc aactctttca atgtgcgaat aacggttgtc aaagatacct
     1621 aacaactttt cgaaataaaa aataacgaca ctttttcagt cagtaaagaa gtgattttat
     1681 gtctgacatg atttgaaatc gagtgccttg gacggataaa ataactttga gcgttccaat
     1741 ggcttcgaca gtttttacag gtatagcgct gcttttttag gcgtataacc attggcatat
     1801 gattatatta ttcaaagtgg acaatggttt ccttcttctt gtttttcacc accattaatt
     1861 tcctatttcc atctaccaca gcagaaccac aatttctaca gacacatgga gcagggaaga
     1921 gaactgcagc aacgatcaat gtttttttct tctgtaaggt ttcgtaagag acctctgtaa
     1981 tcatcaaatt tttgtctatt gatctcagaa tttttttgat agaaccattc ataaagcata
     2041 tcgttctctc agttgtttat tttgtggtga tttaatcata caagaaaacg atatgttttt
     2101 ttataaaaac atgaatagga aaaatagttg acaagaaaat gacaacgcca tatgatgttc
     2161 catagtattt acaacctgtc atagatgact gtaaaaaata aaagtaaaaa gaaaggacga
     2221 caacaggtgg atttttatca gtctctacaa ctagatccgt ttattttaaa acaaaaaata
     2281 cgagaagcag cttctaaaaa agaaaagtgc atgtatattt gttcattgtt tttacgatca
     2341 ttattcatcg tattgtttgc catttgtttt atcattataa ttacaacatt atttgaatcg
     2401 aagcataaac catacgctgt ggttttattc tgtatgttga tgagtattcg ttttgttgac
     2461 tttggttata agatttccca ttcgatcata ggtttagcaa tcgtcatgtt ctcactatta
     2521 gtagcaccgt atgtccagct gatcaaatgg tcagttatgg gcatgttgat tcattttatt
     2581 ttactaagca gcatattaat ggcaaccgct agtgatccca aaatggggaa tgcttctttg
     2641 tacggctttt catatctctt tattgtttat tcgttaccta aagattcgtt gaacaagaat
     2701 ttctttacac aaacaagtag cttattattt ttgttttttt gttggttttc agttctcctt
     2761 tacagaaaac atcgagaaaa gaacaaagac aaaagcttgt ttaaagagat ttttttgaaa
     2821 ggcatgtatt cgcaggaaaa aatttggatg ctcatctatg catttggcat tagtcttttg
     2881 atcgttgcag gagagtatgt cccttttcag cgattgatgt gggcagggtt tgcattttct
     2941 tctattgtat ctagctatgg attgatgtcg attggtttca aagaacgagc agtagaccga
     3001 atcattggct cattgattgg atgtgtttta tttattggta tcagccaatt tattcctttt
     3061 aactgggtcg gtatattagg tggtctagct ttaggtgttt gttctaccta tagatataaa
     3121 acagttttta attcttttgg tgctttggca atcactgcca gtttattcgg ggttccagga
     3181 gcagtaacga tccgcatttt tgagaatatc cttggggtat gtttaggaat catgtatatt
     3241 ggggtgactg aaatacttat ccgaaaaata agagaaaaac acggtttgaa tcattagatg
     3301 agatcgaata aaaaataagg aaaaagtgaa gatacacatg gatgataaat tgctatcatt
     3361 ccatgtgtat ctttttacgg taaatcagtg aaacgttcga ctttcatttt taatttatac
     3421 tttttacgga gttcgtttat ttgagccatc atttcggatt tgtgatgctt ttcgattgct
     3481 tcttcatctt tccaacggtc gatcagtaaa agagattctg gatcttctag tggaaagtaa
     3541 tagtcatatt tctcgtttcc ttcttctgca cgaatcgctt tgacaatgtt tctttcgacc
     3601 atttcttctg caaattttct ggcttgtcca tttgtacttg tatacaaaat attcactgtg
     3661 atactcacta aatcccctcc tgaaaaaatt ctatcactag tatatcacgg tgccagaaat
     3721 tattattcaa gatatgatac acattttttc cgcaatgcta cggaaatata tattttgaat
     3781 agaaaacgct tacaagatat gcttggttta taaaaataaa aggagtgatt gatttatgaa
     3841 aaaagcattg attatttgtg cggcaggaat gtcatcctcg atgatggcct caaagacaac
     3901 ggatttcttt aagcaaaaaa gagaagaggt actcgtagat gcagtttcag ctacagaagg
     3961 cgataatatg attaagaata gtgattttga tttatttctg atcagcccgc aaacgacgat
     4021 gtttttggat aaattcaaaa aattaggaga atctgttgga aagcctgttg ttagtatccc
     4081 tttccaagcg tatgtcccaa tccctacagg catccagaag ttagcagagg tgatcgaaga
     4141 gaacatatga tggaatagga gtggatgata atgaaacaga aggaaaaatt gctgttgaga
     4201 tatttgattg atcacaagaa ggagtatgtc accagtcaac gtttagcttc tgaattgtta
     4261 ttatctgatc ggacaatccg taattacttg cagcggatca aagagttagt agaaaaaaac
     4321 ggaggtaaga tcattgctaa accaggttat ggctaccaac tgcatatttt acaaagactg
     4381 acatttgact tgtttttgag tcggcaagaa atagtacctg gatatgctag agaaccgcaa
     4441 ggattttatg agtccgaaga tcgacaaaag tatatattga accaattgct tttggaagat
     4501 gccattcttt ttatggatga tttggcagaa gagctttata tcagtcgttc cagtttgaca
     4561 aaggacatgc aagaaatcaa ggagcgattg attccatatt cactaaaaat cgtttctaag
     4621 catggtcaag ggacatggat cgaaggggaa gaaagaaatc gacggcattt tatcatggat
     4681 actttttttg gaaaccatta tggaaattca ttgaaagaat atttagggca aagccgtttt
     4741 ttcccagaca tcagttttga agagctagtc attatcattc tcgatgaaac aagagaagct
     4801 aagttaaaag tttcggattt tatcatacag aatctgactt tacacttggc tttggggatc
     4861 aaacggctcc gagaagggtt tgaaatcaag gaattaggca tcgaacaaga agtttctaag
     4921 cgagtagagt atcaagtggc gcagcggatc gttcaacgta ttgaggtaat cgcaggtgta
     4981 tcgtttcccg aagaagaaat tgcttatttg acattacatc tcatggctaa gtcgaatcac
     5041 aaagaaaatc aaaatgatca ggaactatca aaggaattag cgaaagttct gaaaagattg
     5101 tctcaggaat ttcatttttc attagtagag gatctccagc ttttcaatgg actactcgat
     5161 catttgtcac cgatgatgat tcgattgagc cgaggtattc gcattgagaa cccattgact
     5221 gaggagataa agaaagaaaa tcccaaagtc tttgacgcag taaaaaggca tttcagcaac
     5281 atgccggctt tgaaaaacta taccatcaat gaagatgaat gggcctattt ggctttgcat
     5341 ctcatggcgg cattggaaaa agaaagagct gcgcataaac tacatgcatt gattatctgt
     5401 gcaacgggtt acggcagtgc ccaactatta aagaatcgcg tagtcagtga attcggtaaa
     5461 aatattacag tggtaagtgt aaaaggctat tatgagatca atgaaacaac tttaaaagga
     5521 atcgacctga ttatttcttc tattgatctt tcgacaatgt ttttcaaaat tccagtttta
     5581 catgtcagta tttttttgaa tgaagatgat gttcgtaaaa tacgaaaggt agttggagaa
     5641 tctataccct atcattcctc agatagaccg ataccagcat tttctctaca gcaaaaaaag
     5701 cagatttaca ccgaacagtt gtctgaaaaa tttttcaaga cttatacgta cgaaccgaaa
     5761 aaggaagaaa ttcttcatga tttgctgcaa aagctgagca taaatgaaga agaaggatat
     5821 ataagagaaa tgcgaaagca gatccagcaa cgtgaaaata tgggacaaat catattcagt
     5881 gatacagtgg cagtcccaca tcccgctgtt ccagttggaa tcgaaacaaa ggtagccgtt
     5941 gcattgattc cttctgggat gcagtgggat caatatcaag aaattcattt tgttttttta
     6001 gtttcgcctt cttatgtcga aaatgaagga attacggtcg tgactaaagc aatcgttagg
     6061 ttagttgacc agctaaaagt acagcaggaa atcttagctg aacctatttt tgagaatttc
     6121 agcagtaaat ttatcaaact gatcgaataa ggaggaaaac agatggctga aaaaataaac
     6181 agtgaaacac tacaagtgac ttcttttgat atcattttcc atagcggaaa tgcacggaca
     6241 ctgatacatg aagcatttcg ctcgatgcgt aaagagatgt ttgaagaagt agaagagaag
     6301 ttgcgacaag caaatgaaga gattcttcat gcgcatcagt cacaaactca acttctgaaa
     6361 gaatatgcca gcgggacaaa aatagaaatg gaaattatca tggtccatgc acaagatcat
     6421 ctgatgacga cgatgacatt attggaagtt gcacaggaaa tgaattattt gtatcaaaaa
     6481 aataaataga aaggaaggat cattgtgcag gatacagtaa aaaggaaaat cgaattagaa
     6541 aaaaagcaac ttagtttgaa aaacatgtat ttcaatcgtt atctaatcgt acgttacctg
     6601 actgcctttt tctttttcat gaacatgcaa tggatgattc tgcttctatc agctaaaagt
     6661 ttaggaagta gtttaccgat gattctgatt ttagctattt tgcctgcagt aggagaacaa
     6721 gtaaagcttt atcgaaaaca ccaaacgaac gttccatgga caaagtggta ttttttgttc
     6781 cagagtgtct ttaatttatt agtgattcca gtgttgttta cctcaggatt tacattcttg
     6841 tatcctttta tggcaaataa caatggagga cagttatttg ttttcattct tgttgtaagc
     6901 ggtatttttg ccagtgcatt tatacagtat cgtttaaaga aaatttcact caaccaagac
     6961 caacagtata tccgtattaa acaatatgaa aaagcgttat atttaggaaa ggagaataac
     7021 taatgtttga ttttttgcag aaatacctga tgggacctat ggggaaaatt gcgcaactta
     7081 aaatcgttcg ggctgtcatg gcagcgggga tggcttctat tcctttcacg attgtaggct
     7141 cgatgttttt ggttttaaat attttgccat taccgttccc atttttagaa ggatttttta
     7201 atgcaacttt tttcaaagtt agtgacttgt acatgatcgt caatacaatg acgatgggga
     7261 ttttatctat ttactttgcc attgtgtttg cttatgaatt aacgtcaatt gaacgagatg
     7321 aacaaaaatt aaatgttaat ccgctcacgg gggcattact atctgttttt gcattcttta
     7381 tgtgtatccc tgaattgatc atgactgatg gtaaaatcag cttgattgct tctatcaatg
     7441 agacagaaac tgtagtaaat ggtattagga tgggctcttt tgtcgaacgt ttaggaactt
     7501 caggaatctt tacagctatt attatgtcga ttattgcggt cgaattgtat tgtatgtgtg
     7561 tcaagagaaa ttgggtcata aaaatgccag atgttgttcc gccaggagtt tctcgttctt
     7621 tcactgctct gatcccaaca tttgtcattg cgtttgtggt gatgatcatc aatggattat
     7681 tagttgcact aggaacggat atcttcaaaa tgattgctat cccatttggg tttgttacgc
     7741 agttgacgaa tacatggctt ggtgtcatgg tgatttattt cttgatccat gcattgtgga
     7801 tcgtagggat ccatggagcg aatatcatca cttctttctt gaccccgatc gtattagcta
     7861 atatggcagc taacgcacaa ggcgcaaatt atccgttggc aggagaattc aacaattctt
     7921 atgtaaccgt tggtggatca ggtgcgactt taggattgat catctttatt gcattcctcg
     7981 cgaaatctga tcagctgaaa gtgttaggaa aagcctcact tgtaccagga ttattcaata
     8041 tcaatgaacc aatcattttc gggatgccaa tcgtctacaa tccctattta gcggttccgt
     8101 ttttcttagc tccgatggct tctgctacac tcgcttattt tgccattcgg ctggaaatcg
     8161 tcaaaccgat gcttgcacag atgccgtggc cttcaccagt cgggatcgga gcatttgttg
     8221 gtagtggtgg tgattggaaa gctgctgtgt tagcagtcat ttgtgcaatc atcgcattca
     8281 ttatctggtt cccatttatc aaattctatg ataataaatt attaaatgaa gaaagagaaa
     8341 aggcagcaga attagctagt gaaaagttaa aatgatttct aagaaaaaag gaactgtgac
     8401 aaaaaatcac agtccctttt tttcttagaa atgggaaacc actagtatat aaataccatt
     8461 attcttcaat caaccgcttc acgattgcat aatccccttc ataaggttca tctacaccat
     8521 tgattttttg atagggatcc acgccttttc ccgcaataac gactgaatct tgcggagcgc
     8581 tttctgctaa agcgcgtcga atcgcttcag gacgatccat ttcataatga acgatcaaat
     8641 ctggattagt aattgcttca ttgatttctt ccgcgatttt tttcggatct tcaaatgctg
     8701 ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga tagcactttt ccaaaatcat
     8761 gtcgacggga gattgctttg tttcctgggc tgcctaatac aacaattaca cggccatctg
     8821 gatgttcatt tttggcaaat ttcagcaatg ttttcaagct tagataatta tgtgcatagt
     8881 ccacatagac atgcgctcca ttggattgga tcaattgatc cattcggcct ggtacacgag
     8941 cttcttttaa tccgtcttgt gcatcttgac tggaggcacc tgttagtgct gcggcaatga
     9001 tcgcactggc tgcattttct tggttgaatc ctccagccag caaaatatca taagcagtat
     9061 cgcagatagc taagcgatct tcatgagaag ataaagtaaa accgtgagta cctttatctg
     9121 aacggatcac ttgataattg ctttcttccg aacgaccata agtgatcgtt ggaatctgga
     9181 atagatcggc tgtttcccta agtaaatcaa aatagtcact ttcatgattt aaaacaactg
     9241 tttttgaatt caagatcaat tgtcgcttgc aatagaaata gtcatcaaat gtcggatgtt
     9301 cgatcggact gatatggtca ggcgagatat tcaagaagat tccgacatct aaagtcaagc
     9361 cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc catcactaga tgagtcatac
     9421 cattttcaac tgcttctgac atcatacgat ataaatctaa tgactcaggc gttgtcaaat
     9481 gggatttgaa atatgttttg ccatctaacg ttgtattcat agtagaaagc aatgctgttt
     9541 ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta cgcagcagtt gttttacctt
     9601 ttgttccagt gaatccgatc actttcaatt tattttgcgg atagtcgtaa aaagccatag
     9661 caagaagcgc cattgcttta cggatatccg ttacgatgat tcctactgct gaattttcgt
     9721 atggttgttc tgatacataa tattgaatac cctgttcgat tgctgaatcc aaatattctt
     9781 ctttgaaatt cattccttta cagaaaaaca aagtagaagc atcagctttt cttgaatcat
     9841 aacttaactg aagaaattca gctttttctg gtacatcaag atgccagcct cttgttgaaa
     9901 caaattcttt taatagattt tcttttaata gtaaatgacg gatgtcttct aaagagaagc
     9961 tcattgattg atccctcgct ttattcaatt tcttggttta ttatagcaca ctctttttaa
    10021 tatatcgttt gtttccgcta tgacaaaatc cagctttatt caaattttct tgacacaggg
    10081 gacaagcgga tgagtatttt cataaacaat gaactgactg taatagatag gagaacgaat
    10141 agtgtctcca acaatccaaa aacataataa ttcaaaaaga cgataagact attggtaagt
    10201 aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga aaataatcgg cggtatcgtt
    10261 ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa ccccaagact gaacaaaaga
    10321 cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt ccacttttgg aacaatctct
    10381 aaaacaacag gaaaaagcag acttcccgct acagtcttaa aaaatactga ttttcctaat
    10441 aggacagcgg ctagaagcag catcataaga ttgatgctcc aaacagtaac tgagcgttgg
    10501 atagaaaagg ccgtttcaac taaatgtccg atggcgccca ctccggcgga agctatatcg
    10561 tgagggagca agaaccaatt caatccaact gtgataaaga aaagacctaa aaatataacg
    10621 ggtagttttt ttatttcttg tgtcattgat atctcctata aatgttagcc ataaaaggct
    10681 gtatatttct ttactttacc aaaaaaacga aaccgaattc aaaaaaacaa ttgttcttat
    10741 aaaagttata taaaatcgat tgccagtttc tgttttacca attaaaatga tagtaatgtt
    10801 ttgtagaaag ttggaatctg accgatggat aatcaaagaa aaccagcatt agatcaatta
    10861 acaccgcaag aaaaaatggc acgaggctct gcttggatga ccgccagtaa tatcatttca
    10921 agattgctag gcgctgtcta tatcattccc tggtatgctt ggatgggcga aaatgctaag
    10981 gctgccaatg gattattcaa tatgggatac aatatatatg cattgtttct actgatctct
    11041 acagcaggaa tacctgctgc gattgcaaaa caaactgcac gctataattc attgaatgaa
    11101 tatggcactt cacgacgatt atttatccgt gcattacaga tgatgggcgg gttaggcctg
    11161 ttgtttgctt tgttcatgta catcgcctct ccttggctag ctcatgcttc tggtggcgga
    11221 gaagaactga ttccaacgat gcgttctttg agtatcgctg tattggtttt tccttgtatg
    11281 agtgtgatac gagggtattt gcaaggcaat caagaaatga tgccttatgc gctgtcgcag
    11341 attgtagaac aggtggcacg tattttttat atgctattgg ctacattcat catcatgaaa
    11401 gtcatggatg gtgactatgt gacggcggtc acgcaatcaa ctttcgcggc atttatcggt
    11461 atgctagcaa gtattcttgt ccttttgtat tttttgaaga aacatcaagc gtatacatcg
    11521 gcgtttattc actacagcga aaacaaaata aatattgcaa cgaaggaatt gttgttagat
    11581 accgttaaag aagcgattcc gtttattatt gtgggttcag gggtaacgat cttcaaatta
    11641 gtcgatcagt tcacttttat gaagatcatg tctgcatcca ccgagtattc caatgcacag
    11701 ttactggatc ttttttcgat ttttagtgcc aatccagata agttgacgat ggtagttatt
    11761 gcattggcaa catcgatcgc gtctactggg ttaccgttga ttacagaagc tgttacgttg
    11821 aaagatcgag taggtctggc aaaattgaca agcggaaatt tacagttgtt ttcatttttt
    11881 atgtttccgg cgacatttgg tgttatgtta cttgcctatc cgctaaatac attattttat
    11941 acaccggatt ctctaggaag taatgtactg atccaagcaa gttttgtagg actcttccta
    12001 ggactatata tgctagtttc caacatgctg caaggaatgt ttgaaaacaa ggcggcaatt
    12061 aattatttgg ttattgggtt tctagtgaaa ctccttcttc aatacccagc cattcgttta
    12121 tttgaagtat acggcccttt attggcgaca atgatcggat ttgctgtgtc ttgtaccttg
    12181 atcttaaaga gaatccatga agtagcccgc tttaaaccaa gttttgtctg gcgcaggaca
    12241 ttgctgatct ttattcttac cttgattatg cttttggctt cgtggatcac aaagatgatt
    12301 tttggtgctt ttttaaacga agatagcaaa ttccagtctc tattgttgat tcttgtagta
    12361 gcaggtatag gggctttagt gtatggttat ttagctttga aaatccgctt ggcagaaaga
    12421 ctactaggac caggaatagc gcgtttacga cataaattaa aaataaaata agtgaatcag
    12481 aagagctaga ttgatgctct tttgaaatgg gggaaacgca tgcgtttaga taagttttta
    12541 gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat taatcaaaaa aggaacgatc
    12601 gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag taaatgaatt cgaagatgcg
    12661 gttacttatt taggagaaat cattacgtat caaaaagatt tttactacat tttgcataaa
    12721 cctgcaggag tcatttctgc tactcaggat aatcatgatc aaaccgtaat cgatttattg
    12781 gaagaccagg attatcggga agatcttttt ccagtcggcc gattagataa agacacagag
    12841 ggcctcttga ttttaacgaa tgacggggtg tttgcccatc agttactatc acctaaaaaa
    12901 catgtggaga aagaatatct agctgaggtt agcggtataa tgacagaaga agatatccgt
    12961 ttatttgcag aaggactgat tattgatggc gatgagcaga cattgccggc aaaactccaa
    13021 attgatcaag tagatcaaga gacagaaact tcaattatcc gtctgatttt gcatgaagga
    13081 aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa aagaagtctt atatttaaaa
    13141 agaatccgta tgggaaaatt ttggctgcct aaagatttga ataaagggca atatcgaccc
    13201 atgaccaaag aagagctaga acaagtgaga gaaaaataat tgaatggata gctaaaagaa
    13261 taagcagagt agttgtagcg ccatctgaaa agcagacttg tccgtctgac tttcggatgg
    13321 cttttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac cgtttgttcg
    13381 gaataaggga ctgtgacaag acttttgtta cagtccccat tgttccttgt gactcagagt
    13441 tagatagtaa ggacacgatc tccctaatac tcaggccaaa acgctgaatt cacaacctct
    13501 gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    13561 gaactatttt cccgtttttt tctctaatac tcaggccaaa acgctgattt cacaacctct
    13621 cctatacttt gtttcatttt ccctatactt tgtttattta ttctcattat tgaaagcgct
    13681 ataattacgt tgcgaatgaa tcgaaagggg tttttagatg aaaaaacgaa aggggttcaa
    13741 tcattttttt atgaatctat ggcgttataa agcacttgtc ctgatggctg taccgggtat
    13801 gctttggatg atttttttct tttacatacc tgtaatggcg aatgtcgtag cattcaaaaa
    13861 ttttcatatt tcagcagatg ggttcttggc tagcttgagg gaaagtcctt gggtgggttg
    13921 ggaaaatttc aaatttttat tttcttcgga tcaagcattt ctcatcacta aaaatacaat
    13981 tttatataat gtagcattta tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat
    14041 tatgagcgag ttgagaaata aacgactcgt taaagtttat caaacgatgt ccttactacc
    14101 ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa gtcctgacaa
    14161 aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt ggtataatga
    14221 accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga ttggttataa
    14281 cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg aagctgctat
    14341 ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac aacttattcc
    14401 actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg attttggcct
    14461 gttttataac attcctagga attccggtgc attatatgat gtgactcaag ttttggatac
    14521 gtatatctac aatggcctga catccacagg agattttggt atgacggctg ctgcaggact
    14581 ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt
    14641 tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga
    14701 gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat gattgcactc
    14761 tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt taccgatgag
    14821 tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat atttggttat
    14881 acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt cactgtactt
    14941 gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc ttatgcgatt
    15001 tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttgat tacgatgttg
    15061 ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca gttaaaagat
    15121 acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat tgtgatgcgc
    15181 acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat cgatggagct
    15241 agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg tattgcgaca
    15301 atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt actatacatc
    15361 caagatgata agctagttcc actacagtat cttttgatga aaatccaaag taatattcaa
    15421 tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc ctcagttcca
    15481 ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc gatcagctat
    15541 ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga
    15601 aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga agaaactagc
    15661 ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag gttctggttc
    15721 taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa tcggagataa
    15781 accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg
    15841 tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa tgaacgtcat
    15901 catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt ccaatgccca
    15961 aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa aagcttataa
    16021 tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat atgctttccc
    16081 agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt tactagataa
    16141 atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag tcttgaagga
    16201 attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct tcaaagtcca
    16261 agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc tggatggaga
    16321 tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt tacgtacaat
    16381 gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca ataaggatta
    16441 tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt atgattatgg
    16501 cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc
    16561 actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa
    16621 taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat tattgaatgg
    16681 gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag atgataaaat
    16741 caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata caggaaacaa
    16801 caagattcta tatacacaag agtctattac ggatgacatg attgccaaac gtgatcaatc
    16861 gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg acagtgtcaa
    16921 aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat tgaatacagg
    16981 gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca aggctggcta
    17041 tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag aataataatg
    17101 aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg
    17161 tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg gaaagccata
    17221 ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact cttggtaaac
    17281 ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat gaggaactat
    17341 ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc tttttttgta
    17401 taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa ggttttggaa
    17461 gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg gatatataca
    17521 tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa tgaactattt
    17581 tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa tatatataaa
    17641 agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac tgctttattg
    17701 agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc tattgatttt
    17761 attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat gattttagat
    17821 agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc ggttttttcc
    17881 agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg cgtgacttgg
    17941 caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg gaatggtacg
    18001 atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga ataagtttcg
    18061 gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg cggttatcat
    18121 gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg aacaaaatca
    18181 aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa tgctgaatga
    18241 taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg aaaagatcga
    18301 aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg tatacgaaca
    18361 gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa
    18421 ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt attatgaatc
    18481 ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac tagacgcctt
    18541 ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt ttattggttt
    18601 tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg gactctcttt
    18661 atacatgatt tctggaacga caaatcgact atacacattc catgataata aaatagctaa
    18721 agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa attcacaatt
    18781 gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt ctgatgatta
    18841 tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc
    18901 cccgctgatc attggtatca ttattttaga caatatactg cttatctttc tttataaaac
    18961 attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc aaacagcagc
    19021 agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga gggaactttc
    19081 aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca tctacaaatt
    19141 agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca atccacactt
    19201 tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt
    19261 agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc aagagaaaac
    19321 gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt atcaaatgcg
    19381 ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa agatcgaagt
    19441 acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta ttgattttac
    19501 tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat
    19561 catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg aagaaaaact
    19621 gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga atgaacgttt
    19681 acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga caggtggact
    19741 gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt ggtagatgat
    19801 gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt aggctttcaa
    19861 atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca gcgacagatt
    19921 gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt tattgaagcg
    19981 gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca agaattcgat
    20041 tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc cgtgaataag
    20101 ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca aaatgaacaa
    20161 aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa tgaagaattg
    20221 gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca taggatagta
    20281 ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca agaacggcaa
    20341 caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt gttgttggag
    20401 gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga tcaggaatgg
    20461 ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac attccaacaa
    20521 gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca tgttttttac
    20581 gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag acgcgtgctg
    20641 caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga acagtttcag
    20701 ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt tatggagatt
    20761 caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga aaaaatccat
    20821 caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa aaagcatcag
    20881 agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca ccagcattat
    20941 caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt catgtatcta
    21001 ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa ccatttacgt
    21061 atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga gatagcaagt
    21121 gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat cgtcggcaga
    21181 tcccctaaag agtaccgaga aaacagtgga gaatcataaa agaaaaagcc aatgtaatga
    21241 aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt acttgaggca
    21301 aggcagcttt ttcatgacct ctcgataaac ggagttcaaa agccagtttc tcggtattaa
    21361 gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta atactcgaaa
    21421 ctaaacagct ttttcacgac ctctccacta aacggtgttc aatcagctga ccctcggtat
    21481 tagctcaaaa aatggcaaaa tatgaggaac tattttgcca tttgtctctc taatactcag
    21541 gtcagaacgc tgatttcaca acctctcaat aaacggtgtt caaaagcttg catctgcggt
    21601 aataagacca accaaacgaa aaatatgagg atctattttt gcttggctgt tcttattact
    21661 tgatgcagga cagctttttc acaacctctt gttaattata gtttgataag gaaaaaggca
    21721 aatcttttgc ttgatatttc ttagggtgag ggatgatccc taattggtaa gagatcgtgc
    21781 cgcctttcag caaatcttga tgggtaaaat ataatttcgt atattctctg tccatccgtt
    21841 tgattttatg gataaattgt tgctgcggcc gattaggcgt tgcttcaatc gtcaattcct
    21901 ttccatttga caaaagcaaa acagccttgt caattaatgg cataccgata acatattcac
    21961 cggttccagg cgtgacagga taaaatccca gactattgaa gatataccaa gcggccatac
    22021 ttccattgtc ctcatctcca gggtaaccca ttggagaatc gtcgaaggtc tgagtcatca
    22081 actgtttaag cagtggctgt gccatttcag gttgtcctat ataactgaac aagtaaggat
    22141 agtgaaaact tggctggttt gaaatagcta attgtccaaa ttctatcgcg gccatctcac
    22201 tcatttcgtg gatctcaaaa ccatatcctc ctgggtcaaa cgttgggggc tgattgcaca
    22261 gctcaatgag tttttcttta tatctctcac tgccaccata ttttccgatc agtcctgaaa
    22321 aatcgtgata aacagcaaag ctgctttgcc aagcacttcc ttctgcataa tcttgtcccc
    22381 aacgattagc tgaaaatggt tctctgaact tgccatcttt atcttttgaa cgcataaatc
    22441 ctgactcata gtcaaagata ttttgatagt tcaatgattg ctgctgatat ttcttcgtca
    22501 tttcctcttc ttctagtacc ttggcaactt ggctaataca aaaatcgcta taagcatagt
    22561 ctaatgtgtg attgactgat tcgtggtagt gattaggaac atagccatat gtcaagtaat
    22621 cttctgttcc ttggcgtcca taatttggat gtttgctttg aaccgtagca cctttttcca
    22681 ttgccgttag aaattcaggc atcaagtctc gtcggatatc ttttacagca gcatctgcaa
    22741 tgaccgcatc gatcaatgtc ccaggcataa gtccacgttc atcaggagac agccattttg
    22801 gcaaatagcc actttctcta tagctgttca aaaaaccttc cagcatttct tcgtattttt
    22861 ctacagcaat caaagaataa agaggataaa ccgttttata cgtatcccaa aacccattat
    22921 ttgtatagag aacaccaggt tttatagatc gactagtcgt atcatagtgg attttttgcc
    22981 cactaggatc aatttcataa aatgtttgtg gaaataaaaa cacccgatat aaattatgat
    23041 aaaacgtccg caattgttgt tgatcatggt gtgtgagctg tatacgatca aaataggact
    23101 gccattgttc ttgactgtga gcaaggtaag tctctttgct ttggttttgt tctagttcca
    23161 ggttataagc tgcttgttcc gagctgataa aagatgtccc aaatcggata gtttgttttt
    23221 ttgtctgttc aaaatacaac gggatcgagt cgctattttt gttggaaaca gctaactcat
    23281 caattgttaa aggatattca aatctcaata taaaataaaa agaaaaatca ggatcttctg
    23341 aaccagcaaa atgtgtgatt tttccttcca ctgtgtaagg atctataaca aagagctgat
    23401 ggcgtcccgg aaaggataaa agtaaaccgc tgtcattttt gctgtaatca atagataaga
    23461 ttccgccata catgctaggt atcaatgtag aacagatgcc ataacgtaat tgacagatcg
    23521 acagatgagt cgggcaaaat atactttctt ctgggcggta agaactttga gcatggaaaa
    23581 gggtgttctc ctgtagtttt ccattgatcg gcgtaaacag catatgactg aaatcaccca
    23641 tccaaggact tggttgatgg gtaagacggt agccttgaaa aacgcggtca tcaggatgaa
    23701 accaccagct gccgttttga tccgtagttt gcggtgcaaa ataattcatc ccaaaaggaa
    23761 cgccagtata aggctggcag ttcccattag agaaatttgg ctgattcgct gttgcatgtc
    23821 tagtatctat ttgaccgata ttcattttat ttctcctttg ttgattgtct tttgatgaat
    23881 gaaactggaa atttactatc ggatggtcga tcttttgtct ctaaccaagt gaccaaagca
    23941 tctcccgcaa gcttacctaa ctgttcaaaa tcttgtgcga tggttgtcag tggcgggtca
    24001 acaagagctg ctgcttggat atcatcaaat ccgataattg aaaaatcttc agggatatga
    24061 taaccatgct gtctcaagag attcatggtc tggatagcga caacatcgtt ttcacaaaca
    24121 aaagcagtta cttcatactt agtgatataa gaaagcaatt catctaatgt cgcgcttttg
    24181 tcatccagtg aagtgtggaa agtcaaacca gctttgtcca atgcttcaag gtaaccaagg
    24241 taacgctgtc gaacggattg tggatgagtc tgcgtaccaa acaaataagc gatttttgaa
    24301 tgtccctgtt cgatcaaata agaggcagca agcgcaccgc cttggaaatt atcggaaaga
    24361 acggtaggaa aaggtaactc atagattttt ttatctaata tgacgacagg gaattctttc
    24421 aatgaaagct caaaaagtaa gtcaagatgt tgttctgtat caagagcata ataaatcaat
    24481 ccgtcaaatt cattgattag ctcttttgct gttttatttt gtagaaagtc aagcgtcgtc
    24541 atcatgacat caatctgttt atcttgcatc acagggttca atcccttgct aaaatcacca
    24601 acagacaaat cagcgacata tggaagaaga aacagaatcc gtcgtgtttt tgtaggagaa
    24661 gcaagaatct gttccggaga agcggattta acgaaactgc ctttcccgcg aacgcgataa
    24721 ataagtccta attgctctaa ttcagtcaat gcacgtttgg aagtgattct gcttacttta
    24781 taggtacgtg aaagttcttt ttcagttggt agttggctgt ctacaggtaa ttcaccagtt
    24841 aaaatagccg tttgcaaatc attaaatatt gtctggtaca atggttgagt catataaaca
    24901 cctcttaatg atatatcata ttcaaataat accataatgg atgaaaaagt aaataaaaag
    24961 atattttttg aaagcgtaga cataattggc tgtatgaagt ataatcttta aagatatatc
    25021 aaatagaaag aaggttttat tagtggttta taaagatata ccaaaatcgg tgcagaaatt
    25081 tatggaagat atcacacaaa aatgtgggga tatccatgcg gactgggcaa agaattttac
    25141 agcagcattt gccaatactc tgttgacaac agtcaaaaag caaaatgacg gaaccacctt
    25201 tttattgacg ggagacattc cagcgatgtg gttaagagac tctactgcac aagttcgtcc
    25261 ttatttagtg attgcaaaag aagatgagga attagctgac atgatcagcg ggttagtcaa
    25321 aaagcaattt ttttatatca atatcgatcc atatgcgaat gccttcaacg aagaagctaa
    25381 cggtgctggc caccaaacag atcatacaaa aatgaacgat tggatctggg aaagaaaata
    25441 tgaaattgat tctttatgct acccaataca gttggcctat cttctttaca aaaatacagg
    25501 aaaaactgat caattcaatc aatcttttat tgaaggtatc aaaaaaatct tggacgtgtt
    25561 caaaacagag caagatcatg aacactctcc ttatacattt gtacgagaca cgacaagaaa
    25621 agaagatacg ctgatcaatg acgggcgagg tgcaaaaaca gcttttacag gaatgacatg
    25681 gtctggtttc cgtcctagcg atgatgcttg ccgttatgga tatctggtac catccaatat
    25741 gtttgcggtg gtcgtgttag gttatgtgga agaaattttt caaacgatac tcgatgataa
    25801 agaaattgta cagcaaacac agaaattgaa gaaagagatc caagagggga ttcagaatca
    25861 cggacgaacg gttaatcaaa aagggcaaac gatttatgca tatgaagtag atggaatggg
    25921 aaacgccaca gtcatggatg acagtaatgt gcccaatctg atcgccgcac cttacttggg
    25981 atattgttct gaacaagagg aagattatct gcaaacaaga agaacattgt tgagtaaaga
    26041 aaatccatat ttctatgaag gaaagtacgc aaaaggaatt ggatcctccc atacgccaga
    26101 aaaatacgtt tggccgattg cattagctat ggaaggaatg acaacgtcag acaaatctga
    26161 aaaagagcgg atattgaatt tacttgtagc aactgacgca ggaacacacc ttatgcacga
    26221 aggattcgat gtgaacaatc ctgaaaatta tacgagagaa tggttttctt gggcaaatat
    26281 gatgttttgc gaattagtca tggattactt tgatattaga gtagaaaagt aggaggtatc
    26341 gtaaatgaaa aagaaagtat atatcatttc tcatagccac tgggatcgtg aatggtacat
    26401 ggcttacgaa cagcatcaca tgcgactgat tgaattgata gacgatctac tagagctatt
    26461 tgaagttgat ccgtccttca acagttttca tttggatggg caaacgatta ttttagatga
    26521 ctatcttcaa gtaaggccag aaaaaagaca ggcaatccaa caagcaatca atgaaggaaa
    26581 attgagaatt ggaccatttt atatcttgca agatgatttt cttatcagct cggaatcgaa
    26641 cgtgagaaat atgctgattg gcatggaaga aagccgtaaa tggggaaccc ctgtcatgtt
    26701 aggctatttt cccgatacat ttggaaatat gggacagacg cctcaattaa tgaaacaagc
    26761 tggtatttca gcagcagcgt ttggccgtgg tgtaaaacca attggctttg ataatcaagt
    26821 tttggaagca gaaaactatt cttcccaata ctcagagatg tggtggaaag gccctgatca
    26881 aacagcaatc tttggtctgc tatttgccaa ttggtatagc aacgggaatg agattccagt
    26941 agaaaaagaa gcagctcttg ccttttggaa gcaaaaatta gcggatgcag aacaatatgc
    27001 ctctacgaac cacttattga tgatgaatgg tgttgatcat caaccggtac aaaaagatat
    27061 atcaaaagca atccatctcg caaatgaact gtttccagat tacgagttta ttcattctaa
    27121 ttttactgac tatctggaag cagttcaaaa agatgtgcca gaagacttag gctcagtaga
    27181 aggagaattg acaagtcaag aaacggatgg ctggtatact ttagccaata cagcatctgc
    27241 tcgggtttac ttaaaacaat ggaataccaa ggtccagcga cagctagaaa atattacaga
    27301 accacttgcg acgatggctt atgaagttag tggaaactat ccccatgatc aattagatta
    27361 tgcttggaag acattaatgc aaaatcatcc tcacgacagt atttgcggtt gttcagtcga
    27421 ttctgtccat cgggaaatga tacctcgttt tgaaaaagca gatgaagttg gaaagtatct
    27481 tgcacaagat tccttagaac agctgaccgc agcaatcgat acgaccggat tcccaaaaga
    27541 cagctttcca tttgtcattg taaatactgc aggaatggat aaaactggtg aagcagaaat
    27601 aaccatcgaa ttagaaagaa aaagattcgc tgaaggaata cctgaacaac tatatcaaga
    27661 gttagaaaat ttgcctaaac gtaagtatca tgtagaaaca aaaagtgggg ccactatacc
    27721 agctatttta tctgaagaaa ctgtccaatt tggttatgat ttgccgaaag accgtttccg
    27781 tgtgccatat atggcaagaa tgatcaaagt gactttacca ttagaaaaca tgccagcatt
    27841 ttcttgggaa acattcgcgc ttgtagaagg ggaagcgaaa gcagaagaaa aggaaacaat
    27901 gattcatcag agtggacgaa tcatcgaaaa cggccctcta catttgacga ttgaaaaaaa
    27961 tggtaccatt acaatggaag atcgcaaaaa caaacgaaag ttgaataatc tgcatatttt
    28021 tgaagatatc ggcgatatag gaaatgagta tatctttaaa caacctttct gtgatcagcc
    28081 aatcctatcc agtaacaaag aaaactctga agtaaaagta ttagtagata caccggaaat
    28141 agcaaaaata agtattctac aggaaatgga aatccctgtt tctgcagacg agcgtttaga
    28201 aaaagaacag cagatggttg tggaatttcg ttatcgaaaa gcagaacgat ccaaagaaaa
    28261 acgaatcctt caaataaaaa caatcatgac aatcagaaaa gatagtaaaa aaatcgactt
    28321 tgaaacgact ctcgacaatc agatgaaaga tcatcgctta cgtgtcttgt ttcctacaaa
    28381 gttacatgtg gaacatcacg aagcagatag tatttttgaa gtggtgaagc gcccaaacca
    28441 tgtgtctaaa agttgggaaa accccacgaa tccgcaacat cagcaagcat ttgtcaatat
    28501 tcatgatgaa gagtacggtg taacagtcgg aaactttggt ttgaatgaat atgaagtaac
    28561 agaagacgga caaatcgcag tgacgctact tcgcagtgtg ggagaactag gcgattgggg
    28621 atattttcca acaccagaag cacagtgtct aggtgagcat cggttcaatt acagcattga
    28681 actacatgga ccagaagaaa aattctctac ttatttgcat gcatacgcag cacagattcc
    28741 tttttccact caacaaatca aacatcatga aggaacattg atttcaaaac aacaatattt
    28801 aacgataaaa agtgaaacgt ttgcaattac agctttgaaa cgcagcaagt ttagtgataa
    28861 agtagtagta agaggtttca atatgtctag tcatttagaa aaattggaaa taactaaaga
    28921 caatggaaaa actgtcatat tgaatctgct tgaagaacca acgaagcaag cggttgtccc
    28981 aataatccaa ccttatgaga ttcggacaat cggattcgag gaggaaaacg aatgtatggc
    29041 ggaatagaag caggcggaac aaaatttgtg tgtgcggttt cagatggtgg agaaatttta
    29101 gaaaaattaa gtatccctac cacaacacca gaagaaacat tggatcaggt atttgaattt
    29161 tttgatcgtt atgaattgaa gggaatcggg attggctcat tcggcccgat tggtatcgac
    29221 aaaacaaaag ataattacgg atacgttttg gcaactccca aaaaagggtg ggcacaattc
    29281 gattttcttg gcacaatcaa ggaacgctac aatattcctg ctgcttggac aacggatgtc
    29341 aatgctgctg catatgggga attgaagcaa ggagcagcac aaggaaaaaa tagttgtatt
    29401 taccttaccg tgggaacagg aatcggtgca ggtgtcgtga tcaatgaaga aattttttca
    29461 gggatcgcac atcctgaaat gggacatatt tgggtgaaac gccatccaga tgaccattat
    29521 gaaggtactt gtccttatca taaagattgt ttagaaggat tagcagcagg accgtctatt
    29581 gaggcacgca cagggatcaa agggcaagat ctgccgcaag atcatcctgt ttgggctatt
    29641 caggcttatt atattgctca agcactgatc aactatacgt tgacgttagc acctgaaaag
    29701 attattttag gcggtggggt aatgaatcag gatcatctcc ttcaaaaaat ccgtcagcaa
    29761 tttgttgaat tgatgggtgg ttatatggaa acacctcaag taagtgaata tatcgttcgt
    29821 tggggactgc cgaatgaaag tgggattatc ggtagtttgc tgttagcaga acacgaacat
    29881 caaaagtaag cagacaaatt acacaaaaag aacagagtct tatcattatc tgctgataag
    29941 actctgttct tttttatttc tattcccata atccaggtaa agctttaccg actatttttt
    30001 cttgagtaaa tggatggatc atgtgcagtt ctaatgcatg gagcatcagt cgttcagctg
    30061 ttctattttg gtagagaggg tctccaacga tcggatgtcc cttatgagct aaatgaactc
    30121 tgatctggtg tgtacgtccg gtttccaaaa cacaatagac ttgtgtttgt tcagaaagta
    30181 ctttgttgac agtgacatgt gtcaaggcgc tctgaccatt tttatcatct acgacccttt
    30241 ttctccgatc atggcgatct cgtccaattt tattggtgat ggtcatatct ttggcaattt
    30301 ctccccatac gatggcttga tatctccgat agatcatttt ttgttccaac attcttccaa
    30361 gtatcggcaa gacaaatgga ttttttgcaa ataaaacggc accgcttgtt tctttgtcca
    30421 agcggtggac aacgtaaggt cgttgctgtt tagtgtcgag ataagcagct aaatgattga
    30481 gtaaggtact gttttctgtc ggttcatttg gatgagtctt cattcctgca ggcttatcca
    30541 ctataatcaa atgctcatct tcatataaaa cacggatgtg cttcgcatct cctaattgaa
    30601 cttcttgata attataatct gtttcttcga ataacaatgt gatctggtca ttgcccttta
    30661 cttcatgatg gaacaaagca ggttgttgat tgatccaaac gtttttacgt atacgcaaaa
    30721 agtgtcgaac ctttctagga actaaccatt ccttttcaag caagtcgcga attgttgttt
    30781 caggatggtt cataggtaaa gtgatcgtga ttttcacaag tgtactcctt tcagtgcaat
    30841 tgcagactaa ttgtaccatt tttaaaacat atttaagaaa aaacttacat ttttctgttc
    30901 attcttatgt tactctactt gcactaacgt aaaaaaacat attgaactac aacttaagca
    30961 ggaagaaaac actctttcac tatggtaaaa taagacgatt gaatcaaata gacaggagaa
    31021 gaagcaaatg gattttcaaa gcattttaga aaagataaaa gctgctttga tcagtttttg
    31081 gcgttggatc agaccatatc tcggacaatt ccatcgatgg cgaaagcgca tctggaaaaa
    31141 atatcatgtg aacaaattga ttttgctgat tggtttgatc tgtgtcttag tgacaagtat
    31201 ctacctcttt attttagcaa aacaagcaaa tgtcgaaacg ttaaaatcag gattaagcca
    31261 atcgacagtc atttatgata agaaaaacga agaagctggc acactgtacg cccaaaaagg
    31321 aacatatgta gaattaaacg cgatttctcc ctatatccaa gatgcagtaa tctcgacaga
    31381 agataggaat ttttacgagc atcacgggtt tgatatcaaa gggattgccc gtgcagcagt
    31441 ccgaatggtc ttgtctggcg gaacatcagg gggcggagga agtacgatca ctcagcagct
    31501 tgccaaaaat gcgtatctga cattagacca aacgtttgat cgaaaagcga aggaattatt
    31561 tttggcaatc gagatcgaaa agaagtacag caaagaagaa atcttgacca tgtatcttaa
    31621 caatgcctat tttggcaacg gggtatgggg tgtccaagac gcttccagaa agtattttgg
    31681 agtagatgca acgaatgtta cgctgtctga ggctgctact ttggcaggaa tgctgaaagg
    31741 tcctggtatt tataatccga tcgatcaccc aaaaaatgcg aatgatcgga aaaacacagt
    31801 actaagtgta atggaagaaa acggaaaaat cacaaaagaa caagaacaag cagaaagcca
    31861 gacagatatc agcgcgtatc ttaatgacac atacgataat tcagattctg gttatcgtta
    31921 tccttattat tttgatgcag tgatcgatga agcagttaac gagtatggtc ttgatgaaga
    31981 agatgtcatg aataaaggct ataaaatcta tacagcttta gatcaggatt atcaaaagca
    32041 attagaagct acttatcaaa acagtgcatt attcccagca aatgcttcag atggtgccat
    32101 ggtccaatct gcttcagtag ctttagatcc gaatacaggt ggtgtacaag cactagtagg
    32161 acgaagagga gatcatgtct ttagaggatt cagctttgct acacagatgc gtcgttcacc
    32221 aggatcaacg atcaaaccat taagcgtata tacaccagca ctggaagctg gatacaagcc
    32281 ttccagtatc ttgaaagacg aaccgcaaag ttattacgat gcgcataatt ttgacgggac
    32341 ataccaagga gaagtaccga tgtatcaagc tgttgcacaa agtttgaatt tgcctgctgt
    32401 atggcttttg catgagatcg gtttacaaaa aggatatgat aaagcagaag aattcggcct
    32461 gcctttagca aaatctgata agtattatgg cttagccctt ggtggactgg aaaaaggagt
    32521 atctccattg atcatggcgg gtgcttacag tgcatttgcc aatgatggtc agatgtatac
    32581 gccgcatctt atcacgaaaa tcgttgattc gactggagcg gtgatcgtcg ataacacaaa
    32641 caaaaaacca aaacaggtca tatcaaagga aacagccgat gaaatgacca gcatgcttct
    32701 tggtacattt tcaaatggaa cagcggctgc tgcgaatcct gcaggttata cgatagcagg
    32761 gaaaacagga accacagaaa ctaatttcga tgccacaaaa gcaaatgacc aatggatgat
    32821 cggttacacg ccagacgtag tgatttcgac atggatgggt tacgaagaat ccagcaagct
    32881 gcactatttg gaagggacaa gtggaaatgt tgtcgggaaa gtcttcaaat cagcggcaga
    32941 aggcatcctt ccgtatactt caaaaacagg atttagtgta gcagatgcct atgccacagg
    33001 aggacaagtg gtagctgcag atcaagttgg aaacacagga aattctaatg gagagtctgg
    33061 aaattggcaa gataacttga atcaatatgg tgaacaagca aaagaagggc tgaaaaactt
    33121 cggcgatatg gtgaaagaag gcgttcaggg aattggtgaa gcggctaaag acctctggcg
    33181 gaaatatcaa ggagagtagc ccctttgaaa acgaataacc ccatgttaca atagagagta
    33241 actactaaaa gaaaagaggg cataacatgt caaacatcta cgatagcgcg aatcaaatcg
    33301 aacgtgagat ccgtgaatta ccagaattca aagcgttgaa agaagcgtac gagaaagtac
    33361 aagcaaacga agaagcacac aaactgttca aagacttcca agcgatgcaa atcgaattac
    33421 aagaaaaaca aatgagtgga caagagttca gcgacgaaga tgcaatgaaa gcacaagaaa
    33481 tggctgtgaa aatccaatca gaagaagtaa tcaatgactt gatgcaaaaa gagcaaggct
    33541 tcagtacaat catcaatgac ttgaaccgta tcatcatgac accagttcgc gacctttaca
    33601 gcgaataaaa ctattttatg aaacaacaga gggtgtgaaa gagacgttcg gccttgagcg
    33661 atgacaaaat tgcttcttat tgccgatagg ctgtctcttg aacaccattt accaagaggt
    33721 cgtgagccta ccgtttaact ctgagttaca aggaacaatt tcaaaaaaca gcttctcatg
    33781 tttgatgact attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcagaa
    33841 ataggagcta cgacaaaagt cttgtcatag ctcctatttt ttgtcattca tttttcaagc
    33901 ggtagaaata gcctttttga gctaacagtt tttcgtaagt acctatttca acgatttttc
    33961 cttcatcgat cacaacgatt ttatctgcct ctttgatcgt tgaaagccga tgagccacaa
    34021 taaaagtagt gggtgtttct gtcaggtaat tcagggcttg ctggattttt ttctctgact
    34081 ggttatctaa agctgaggtt gcttcatcta gcaaaatgat ttttggctga cgtatcaacg
    34141 ctcgtgcaat ggagatacat tgcttttggc caccagacag cttgttgcca tgttcgccta
    34201 ctaaggtatc cagaccatca ggaagctggt caatcagtga agaaagttgc gcagcttcga
    34261 tgacttcatc taaacgttct tttgatacgt ttttcaagcc ataagtgatg ttctctttga
    34321 tcgaagcgga gaacaacaaa gtggtttgag gaacaacggc taaataattt cgcacacttc
    34381 gcatatccag ttcatccaaa ggctgatcat ctagaaaaat ttttcctgaa gtcggctgga
    34441 taaatccaat caataagtta aggagtgtag attttccaga gccagatggc ccaacaaaag
    34501 caattgtttc tccagctttc acctctagag aaaaatgatc gataatcggt ttgagtgagt
    34561 ccggataaag aaaagaaaca tcctcaaaac gaaaatttcc atccaaatgt tggatttttt
    34621 tcttaccgtg ataagtttct acatcgccag caatcaacag ttcgccaata gaagtgatag
    34681 attccatgcc ttttgaaatc gtaggaatca gcgtgatcat tccagaaacg gaattgacga
    34741 tcgtcgtgaa atacgtctga tacatcacga catcgccggg ttggatgcgc ccttgtaaag
    34801 ctaatatccc agtgaaaatc aaacaaatca gctgaaatat ctgaaaacta gcccagctga
    34861 ctgatccgaa aacagattgc aggatatcta agtggaatcc tttttcatag atttgagtaa
    34921 aatgcttatt taagcgagtt gtttcgtgtt tttctaaccc atgagctcga gcaatcggtg
    34981 ctaattcgat cacttcgatc acttttgcag aagtctcttc catttcttgc ctgaagtctg
    35041 agttggactg tctgatcctt ttacggaaaa agagagtaac gaatgcggcg agaggaacag
    35101 tcaataagaa aaacatgaaa acaacaggac ttttataaag cgtgatacct aaagctacga
    35161 ataaattgac agcgatattt aatccgctga caaatacttg agaggacaac gtttggatag
    35221 cttctacatc acggatgatc tttgactgga ttcgtccaga ttgcaattct ttttgataag
    35281 gaatagatag ttcttggatt ttcttgataa gggtcgctct tagtccagct tctacttgac
    35341 ggatcgaagt gctatggtat tttacatgaa aataattcgt caatatattt tgaccaatca
    35401 gacaagccat caaaaaagcg ttgaaataaa tggcctgcaa ctgagaagaa tctttcgatg
    35461 aagctgcatt gattacattg gcaatagcga tcggcattaa ccaagatgga agatgtttga
    35521 taataaaaaa gagtcctgac atggcaaaac gccaataatt tccctgataa agatgcagca
    35581 atgtctgaaa agcatttgca tgatattttt gataactttt ctgtattcgt tcctccataa
    35641 tcttctcctt aacaatcttg gtaaataaac tgcaagtgtt ctattgcttc aggaacataa
    35701 atctcaggag tagcttcaag agtggcgtac attagaggac gctcatattt gatataatgc
    35761 agtaccgggc ggaaatctaa acagcccttg ccaacaggta ccatgcggat ctttccattt
    35821 tccatgatat agtctttgag atgaagcgct gtaatataac catgtaaatt gtccaaggca
    35881 cctcggatca ctttttcctg atcagcatga ttttctttat gcatcaaatt cgcacaatct
    35941 aaaatgattt ttaaatttgg tgaagctact tcatcgatta aacgtttagc taactgatag
    36001 ttatagattg gatgattgat ccctgcttca acagcaacag ttacgccaaa attttctgca
    36061 tgagcgacta attcaatgat agaatttctg gcgatctgat aagcttcttc agtaaaattt
    36121 tcttttgtgt aaccgacaga gacactaccg gtttccgtag caatcatctt ggcttgatag
    36181 gcagagcaca gagcaatttg ctgtttgaaa gttgccaatg cttgtgcacg aacttcttga
    36241 tttttgctgg ataggttgat atagcatcct aaaatggaaa gttcgatatt gtttttttgg
    36301 aagtattcac caaaataggc agcactccca agtgtcatgt cagttaattt aagttgttca
    36361 ggaaatgctt tttgtggtgc aaattggata tgggacaagt gaagctgtga tactttttga
    36421 acgatttctt cacgatttcg aacatctaaa tcgtgggcac ggatgcctaa atttaatgga
    36481 aacatctcaa actccttttt gtaaataatt agcaagaggc agacgggatt cttttatcaa
    36541 ttcaacaatt attcgggcaa ctttatcagc gccttctgga ctaaagtggg tattgtcttt
    36601 tagtccttct gggtaatttt tacaagtatc aggcataaga tgcaggtaga atttttttgt
    36661 ctcttctttt gagaaggtct ggaacaattc ttgtgatctt gagaacacat ctaaaagagg
    36721 aatatggtgt ttctcagcaa atgcacgcat cgcttttgga tagtctccaa gtatatctgg
    36781 attcaatgtt ccgttttcta agtaatctcg tcgcgtcaca gatgtcagta agaccatttg
    36841 gacgttcgct ttttttgcgc attcaaaaaa tatctgcaga ttttttaaat agctaccgaa
    36901 gggaagcgta cctctttctt cttggatttt ctggtcgtta tgtccaaatt gtacaaataa
    36961 gaaatcattc ggctgaaact gtgaagctag acgggctaac cttccttcct ggataaatga
    37021 tttggtgctg cgtccattct ttgcttggtt attgatctgg atctcgggtg aaaaataagc
    37081 agagattttt tctccccaac ctgtctcggg acgcttgttt tcatcttttt ctgccgcagt
    37141 agaatcaccg gcaatatgga tagtaggcat gttatctctt ctttcataaa ggtttatagg
    37201 caatgaagca gaacagcaag agaagaagac ggagcaa
//
LOCUS       DACDKL010000010        35199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDKL010000010 DACDKL010000000
VERSION     DACDKL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.010"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(261..746)
                     /locus_tag="IV329_000393"
     CDS             complement(261..746)
                     /locus_tag="IV329_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8939601.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1226..1867
                     /locus_tag="IV329_000394"
     CDS             1226..1867
                     /locus_tag="IV329_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8939602.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1886..2521
                     /locus_tag="IV329_000395"
     CDS             1886..2521
                     /locus_tag="IV329_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8939603.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2539..3354
                     /locus_tag="IV329_000396"
     CDS             2539..3354
                     /locus_tag="IV329_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8939604.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3673..5109
                     /locus_tag="IV329_000397"
     CDS             3673..5109
                     /locus_tag="IV329_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8939605.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5298..7745
                     /locus_tag="IV329_000398"
     CDS             5298..7745
                     /locus_tag="IV329_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8939606.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7975..9042
                     /gene="rlmN"
                     /locus_tag="IV329_000399"
     CDS             7975..9042
                     /gene="rlmN"
                     /locus_tag="IV329_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8939607.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9235..10338)
                     /locus_tag="IV329_000400"
     CDS             complement(9235..10338)
                     /locus_tag="IV329_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8939608.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10891..11439
                     /gene="aac(6')"
                     /locus_tag="IV329_000401"
     CDS             10891..11439
                     /gene="aac(6')"
                     /locus_tag="IV329_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8939609.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11985..13646
                     /locus_tag="IV329_000402"
     CDS             11985..13646
                     /locus_tag="IV329_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8939610.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13716..14063)
                     /locus_tag="IV329_000403"
     CDS             complement(13716..14063)
                     /locus_tag="IV329_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8939611.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14147..15088
                     /locus_tag="IV329_000404"
     CDS             14147..15088
                     /locus_tag="IV329_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939612.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15089..16132
                     /locus_tag="IV329_000405"
     CDS             15089..16132
                     /locus_tag="IV329_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939613.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16138..17208
                     /locus_tag="IV329_000406"
     CDS             16138..17208
                     /locus_tag="IV329_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939614.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17198..18151
                     /locus_tag="IV329_000407"
     CDS             17198..18151
                     /locus_tag="IV329_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8939615.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18163..19173
                     /locus_tag="IV329_000408"
     CDS             18163..19173
                     /locus_tag="IV329_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8939616.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19458..20279)
                     /locus_tag="IV329_000409"
     CDS             complement(19458..20279)
                     /locus_tag="IV329_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8939617.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20376..20654)
                     /locus_tag="IV329_000410"
     CDS             complement(20376..20654)
                     /locus_tag="IV329_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939618.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20922..22931
                     /gene="metG"
                     /locus_tag="IV329_000411"
     CDS             20922..22931
                     /gene="metG"
                     /locus_tag="IV329_000411"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8939619.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23410..23997)
                     /locus_tag="IV329_000412"
     CDS             complement(23410..23997)
                     /locus_tag="IV329_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939620.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24172..25494
                     /locus_tag="IV329_000413"
     CDS             24172..25494
                     /locus_tag="IV329_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8939621.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25649..26425
                     /locus_tag="IV329_000414"
     CDS             25649..26425
                     /locus_tag="IV329_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8939622.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26418..26987
                     /gene="rnmV"
                     /locus_tag="IV329_000415"
     CDS             26418..26987
                     /gene="rnmV"
                     /locus_tag="IV329_000415"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8939623.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26980..27864
                     /gene="rsmA"
                     /locus_tag="IV329_000416"
     CDS             26980..27864
                     /gene="rsmA"
                     /locus_tag="IV329_000416"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8939624.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28315..28944
                     /locus_tag="IV329_000417"
     CDS             28315..28944
                     /locus_tag="IV329_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939625.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29068..30177)
                     /gene="ugpC"
                     /locus_tag="IV329_000418"
     CDS             complement(29068..30177)
                     /gene="ugpC"
                     /locus_tag="IV329_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8939626.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30386..30808
                     /gene="mgsA"
                     /locus_tag="IV329_000419"
     CDS             30386..30808
                     /gene="mgsA"
                     /locus_tag="IV329_000419"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8939627.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31131..31667
                     /locus_tag="IV329_000420"
     CDS             31131..31667
                     /locus_tag="IV329_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8939628.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31707..33470)
                     /locus_tag="IV329_000421"
     CDS             complement(31707..33470)
                     /locus_tag="IV329_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939629.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33473..35188)
                     /locus_tag="IV329_000422"
     CDS             complement(33473..35188)
                     /locus_tag="IV329_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939630.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        11449 a         6273 c         7294 g        10183 t
ORIGIN      
        1 tattttttgt taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa
       61 agaagcattt ttatatatgc ttcttttttc gtagaatcaa taaatatgga tttggctaac
      121 tatggaagaa tactcgtatt agttttcagc gttcatacta gttgttacat tgtttgattg
      181 gccaaaagaa tttctgatat attttgtgct aaataaagaa aaagacgctt gaattgggaa
      241 ataagaaaac tactctatca ttacaaattt tcataaactg gcagatgttc taagtaaaca
      301 agtatctttt caaagtcgga aaaatccata atcaacgtag tggtattgat atttggatga
      361 aagcctacgg tatcttgacg atcaatagca gcatcgatca cgacttggat ttttttctct
      421 ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg gaacgcctag gaggtcagga
      481 agctgttctt cagaaacaaa agataagcgc ttttctttta attgttcagc tagttgtttt
      541 aaatcggcgt gcttttcatc aggcaagatg acaagataga tttgttttcc ttttcttgat
      601 ttcaataata gattttttac ttgcggacca ggtagggaaa aagggacatt tttgactgac
      661 gtaatcgcag gatgatcgac tttttgaaaa gagataccca gtttttttag tagttcataa
      721 gcttcttgtt cggttgcacg actcatcgcg catcaccctc ctttttttat tagtgtatca
      781 taaaaaagag ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt taaaacaaat
      841 gacatcgaaa agaagagtag ccgttaggtg tttttagaga gtctctggtt ggtggaaaga
      901 ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa
      961 atccggggag cccgttacag ctgcggtcgt tgctagatga ccagataagg ctggcttttc
     1021 cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga tcttcggatc
     1081 aaaggacttt ttgttttcat ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa
     1141 aaaataaaca gaggtggaac cacgtataat acgtcctctt ggcatttgcc aagaggattt
     1201 ttttatttta aaggaggaaa agaagatgga gtatttgatt gaagttatgc cgcaattatt
     1261 agtaggagca ctcacaacat tgaaattatt tttatttacc ttgattggct cattaccatt
     1321 agggattgtt ttagcattag gattgtcttt gcattttaag ccgctacgct acttgcttca
     1381 aacatatgta tgggttatgc ggggaacacc gctgttgttg caactgattt ttatttttta
     1441 tgggttgccg acgattggga tcgtttttga tcgtttcgaa gcagcactca ttgcctttat
     1501 cttaaactat gcagcttatt ttgcagagat ctttcgtgga gggatacaat cgattcctca
     1561 aggtcaatat gaagcagcgc aagttttgca attaagtccc tttcaaacgg tgacgaaaat
     1621 cgtcatccca caagtgacaa agattgtttt accttctgta gggaatgaag ttatcaactt
     1681 ggtcaaagat tcttccttga tctatatatt aggtttaggt gacgtaatgc gtgctgggaa
     1741 aatcgcgatg gaacgcgacg cgactttact gcctttgcta ggcgtggcaa tcatctatct
     1801 tacattgact ggtattctaa caatcataat gaaacaagtt gaaggaaaat taaattatta
     1861 ccgctaactg ataggaggaa gaaatatgat tgaattgaag aaagtgaaca aagcatttgg
     1921 tgaaaaaaaa ataatcaaag atttgaatct aatcattcca gatgggaaaa cattagctat
     1981 cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt atcttggctg gcttagaaag
     2041 ttctgacagc ggagaatttt atttagacgg tgtaccgttc aacccattcg agcttaaaat
     2101 gaaagaacaa gttgtaggag tcgtattcca agacttccag ctatttccac atctgaatat
     2161 ttttgaaaac attaccttag caccaaaaat ggtattgaaa cagacaaaag aagaatacac
     2221 caaaaaagca gaagaattag cggcttcatt agggattagt gatttattga atatctatcc
     2281 ttatcagctt tctggcggac aaaaacagcg ggtagcgatt gccagagcat tggctatgaa
     2341 tccacgggta ttggcttatg atgaaccaac aagtgcttta gatcctgaat tacgccagca
     2401 agtcgagcgt ttgatcctat cattgaaaga agatggggta acccaaatta ttgtcaccca
     2461 tgatttagta tttgcagaaa atgtcggtga tcaattacta aaagtttccc caattcatta
     2521 agaaaggaga agatagagat gaaaaaaacc agttttctat tcgctttgat cgcaggtctt
     2581 cttctttttg caggctgttc gaacaaaaaa acttcagcag ataattgggc gactatcaac
     2641 gaagagaaaa aaatcgttat aggtttagac gatacatttg ttcctatggg atttagagat
     2701 aaagacggga agctcacagg atttgatatt gatttagcca aagcagtctt tgaacaatat
     2761 gggattacgg tagactttca gcccattgac tggtcaatga aggaatttga actgaacaat
     2821 ggaacgatcg atttgatatg gaacggctat tccaaaacgc cggcaagaga aaagaaagtg
     2881 caatttacaa aaccttatat ggaaaacgat caagtgctga tcacacctaa agaatcagga
     2941 atcacaaact ttggacaaat gaataataag agattaggtg cgcaaaatgg gtcatctggt
     3001 tatgatgtat ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa ccaagatgct
     3061 gttttgtatg atagttttaa tgaagcattg atcgatctga aaagcagacg ggtcgatggt
     3121 ctgctgatgg ataaagtata cgcagactat tatttgaaac aaagaaacga actagaagac
     3181 ttcaacataa caaaaggttc ttatgagagt gaggattttg cagttggtgc acgcaagtca
     3241 gatagtacat tagtgaaaaa aatcaatgaa gccctagatc aactagagaa aacaggtgaa
     3301 ttccaaaaaa tatccgataa atggtttggc gaagatgtta cgccgagaaa ttaacaaata
     3361 ggggactgtg acaaaagacg tgtcacagtc cctttttccg aataaacagt gttctcctgc
     3421 caactccttg tgactcggag ctgaatggca ggctcacaac ctcttggtaa acggtgttca
     3481 aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca
     3541 aaatttttct tactactcga aactaagcga ctttttcaca acctccttta cataatttta
     3601 ttttgttgtg gaaggggttt cgtgttattc taaaagagga acgattgaag gttataggaa
     3661 aggaagagat gaatggaaca caaagaactt acctcatttc caaaagattt tctgtggggc
     3721 tctgcttctg cagcgtatca agtagaagga gcttggcaag aagatgggaa aggagaatca
     3781 gtctgggatc gatttgtacg gatacctgga aagacattta aaggaacgaa cggtgatttg
     3841 gcagtagacc actatcatcg ttataaagaa gatattgctt taatgaaaga acaaggtctg
     3901 aaagcttacc gcttctctgt agcttggaca cggatttttc ctaatggacg aggagaagtc
     3961 aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg aattgattga aaacgatatt
     4021 gaacctatgt tgactttata tcattgggat cttccacaag cattgcaaga tgaatataat
     4081 ggttgggaat cacgtcagat cattgaagat ttcactcatt atgcagaaac attatttgag
     4141 gcattccgcg ggaaagtcca ttactggatt agtttgaatg aacaaaatat cttcacttca
     4201 ttaggttatt tgttagcagc acatccgcca ggtgtgacag acccgaaacg aatgtatgaa
     4261 gttaaccata ttgctaattt agcaaatgca tctgtcatca ataaatttca cgagttagaa
     4321 atgcctggaa aaatcggacc aagctttgcg tatacgccaa attatccaat tgacagtaat
     4381 cctgaaaatg ttctggctgc tgaaaatgca gaagacctga tggccaatta ttggatggat
     4441 gtctatatgt ggggaaaata tccgattgct gccatgaggt tcttagaaga aaaaggatgg
     4501 gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc agattttctt
     4561 gggatcaatt actatcaaac ggcgacgaat gcattcaatc cattaaacgg agttggtgct
     4621 ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag aaactggagt tcctggaatg
     4681 tataaaaaag ttgagaatcc atttgtcgaa cgaacaaatt gggactggga gattgaccca
     4741 gaaggattaa ggatcggact gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt
     4801 acggaaaatg gcttaggaga gtatgacaag ttgacagaag ataaacaaat ccatgacgac
     4861 tatcgaatca attacttaca aagccacgtc aaagccatca aagaagcaat ttcggatgga
     4921 gcagaagtcc taggctattg cacctggtct tatacagatt tgcttagctg gctgaacggt
     4981 tatcaaaagc gatatggatt tgtatatgtt gatcaagacg aaacacaaga aggatctttg
     5041 gaacgatata agaaagacag ttattactgg taccaaaaac tgattgaaga aaatggagag
     5101 aacctttagt tcacatactt aaaacaagaa ggtacggctg gatatggccg tgccttttct
     5161 tgataaaatc atttagaaat taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc
     5221 gtcagttctt tcaaacaaaa ctttagttcc cttacttttt tttgtataat gaaacttgat
     5281 gaaatgaggg ataataaatg gcacaaaaga aacggcctac taaaaaaagg aaaaagacga
     5341 aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct gacgattggt gttgggtttg
     5401 ccttgatttt atttgctgtc ttcggctttt taaaattagg ttttttagga atattgattg
     5461 caaacggctt tcggatcatt gcaggaaata cttaccaaat cctctgtctt ttattagccg
     5521 tattaggatt ttggattgtc attaagaata cagagttttc aattggtaaa aaccgacgct
     5581 ggtttggcgg catactcttt tatttcggta tcttattatt gttacatgcc catttatttg
     5641 gcaaactcca tactggggaa ccaaatatca tggggacgac atgggatttg ttagccagtg
     5701 atataaaaca aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt
     5761 atcattttac ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac
     5821 tggcaggagg agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc
     5881 aatcaattgg agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa
     5941 agcaagcggc aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct
     6001 tagctgcaaa agaagctgca gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga
     6061 gccagcaaag ggaaaatact gctgatgaat ggcaggaacc agaaaataca gagccggagc
     6121 agctaagttt tgttccaatc gacagttttc aagaaaatat ccatcctgcg aatctagaga
     6181 aaccagtacc agatacacca aaacagacca atacagcaga aggtttttca gatgagttgc
     6241 cagaggatga cggtacctca ttagaatttg agattgaagc agaacaggaa aatcaagact
     6301 atgaattacc gacagttgat ttgcttgatt ctataccaac tgtggatcaa agtgatgagt
     6361 ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg
     6421 atgccaaggt cgtcaaagcc agcttaggac catccgtcac aaaattcgaa gtccagccgg
     6481 ctgtaggggt aaaagtaagt aaaattgtga acttaacaga tgatatcgcg cttgctttag
     6541 cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa atcactgatt ggtattgaag
     6601 taccaaatgg aaagatcagt atggtttcct ttagagaaat catcgaagca caaccaaacc
     6661 acccagataa attactagaa gtacctttag gacgtgatgt ttctggacgg gtacaaacgg
     6721 ctgatctttc gaagatgcct cacttactcg tggctggttc gactggtagt gggaagtcag
     6781 tagcaattaa tgggattatc acaagtatat taatgcgtgc taagccccat gaagtaaaac
     6841 tgatgatgat cgatccgaaa atggttgaat tgaatatgta caacggcata cctcatctgt
     6901 tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag
     6961 aaatggaaga acgatatgaa aaattcgcgg caaccggagt aagaaatatt tctggttaca
     7021 atgaatttgt tcaacagaaa aacttggaaa acggaacgaa acatccaacc ttaccattca
     7081 tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt agccagcaat gaagttgaag
     7141 atgcgattat tcgtttagcg caaatggctc gggcagcggg gattcatatg atcctagcta
     7201 ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa agcaaatgta ccttccagaa
     7261 tggcatttgc ggtatcaagc ggtacagatt ctcggacgat cattgattct aatggggcag
     7321 aaaagctctt agggcgagga gatatgctat tcttgccaat gggtgagaat aaaccgatcc
     7381 gtgtgcaagg agcattcatt tctgaccacg aagtagaacg ggtcgttcaa tttgttacag
     7441 atcagcaaga agctcattat gaagagaaaa tgatgccgac agatgaagtt gaaacgacag
     7501 gtgctccaga acagccacaa gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa
     7561 tgcaaacggc aagtatttcc cttctccaaa gaagatttcg gatcggttac aaccgagcag
     7621 cacgtttagt agatgaactg gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc
     7681 ctcggaaagt attcattgag caggaagaaa catcagaaac gttgccggac gataatgaca
     7741 tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc aataaaaaga caaggcatga
     7801 tgataccaaa agaaaaaatg gtttttttcc atcttttcta atacggtatg atgtcttgtc
     7861 tttttagttt ttgtgagatt aaagttctat tcgatttatt tgtgaagtat tgcattaagg
     7921 aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa agagggaaaa tactttggaa
     7981 aaacagtcca tttatggctt gacgaatgaa gaattaatca attggtttat agaaaacggc
     8041 gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat
     8101 ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa aattatcgga gcattttatc
     8161 atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg
     8221 tttgaactcc ctgataaaaa catgattgag actgtactga tgagacaaga atatggcttg
     8281 tcagtttgtg tgacgacaca agtaggatgt aatatcggct gtacgttttg tgcaagcggg
     8341 ctgctcaaaa agaaccgtga tctgactgct ggagaaattg ttgcacagat catgatggtc
     8401 caacattatt ttgatgagcg caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg
     8461 atcggtgagc catttgataa ttacgacaat gtaatggact ttttgcatat catcaatgat
     8521 gccaaaggat tggcaattgg tgctcggcac attacagttt ccactagtgg attagcacac
     8581 aaaatcaagg aattcgctaa taacggcttg caagtcaatc tggctatttc cttgcacgcg
     8641 ccgaataatg aggtgcgaac atcgatcatg cggatcaacc gaagtttccc aattgagaaa
     8701 ttgatggaag ctgtggatga gtatttagag aaaacaaacc gtcgaatcac gtttgaatat
     8761 atcatgttga atcaagtaaa tgatagacca gagcatgcgc aacaattagc agatctatta
     8821 aaagataaga aaaaattagc atatgtcaac ttgattccat ataatccagt cagtgaacat
     8881 gatcaatatt ctcgcagtcc aaaagctgat gtattgagat tttatgatgt attgaagaaa
     8941 aacggcgtaa actgtgtgat acgtaaagaa catggtacag atattgatgc agcttgcgga
     9001 caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt
     9061 ttcaattacc ataaaaggag cagttttctt aattaaggaa ctgttcaact taaataagag
     9121 aaaagattct atagcgaaag ctataaagct ttttttgaca agaaaaaccc tcatcaagca
     9181 aagaaatgtc ctcaatttga tgacagcctg cttgatgggg gttttagcaa ttatttaact
     9241 gattttctta tatccttgct ggctttcttg tgtagcatga taagtttcta cgtcaagaat
     9301 ggcctgttta aatttttctc gggtgatttc ataaggcaca tgtttaatat catttgttgc
     9361 catggcttta tctaagattg cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa
     9421 cgatactgga agttggttct ttttcataaa cacatagatt ttatcaaaca aatcgaactg
     9481 ctgatccatc atcaataagg tcagtacacc ataactgaca gatgctccgt gaaggtatcc
     9541 ctccattttt tctagaacag tcgtaccgta atagaatgag tgtgccatag cagaattata
     9601 gttattgtta actaaaacag aaaccaatcc ggtagtaaca atgatatccg aaacgacttg
     9661 ttcaattgca tatgagggac gatcgtcggc actatctttc aatgcttgcg taccataagt
     9721 caagagacga tcaatgcaca tttttccgat ttggattcct aatctatccg tgtagcctag
     9781 atcatcgcca cgagcagaaa aagtagattc gacttctttg gataaagcat cgccaatacc
     9841 tgcccataag tatttatcag gtgcttcggc aataattgct gtatcaatga aggtgtgtac
     9901 aggtggtttt gaaggaaaga agagtccgtc cattacgcct tcttcgttat agatgacaca
     9961 taccgcagtg acgggcgaac agttcgaacc aatagtggga aaggtgaaga ctggtttagc
    10021 tagtttttca ccaatgactt ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag
    10081 gacgtctgct ttttggacac tttcatgctg gatcaactgt tcaatatttc cataagttga
    10141 attacccccg taccagagac tggcgacaac ctctatttcc tgtgtcaaag ctacttttat
    10201 tttgttttct gcctttgaaa gtgcagtctt ccctccaatc agcactactt ttgtcccata
    10261 ttctttgacg attccatttg ctttttcata cgcatctgtt ccaatggtat aatttggtaa
    10321 gaaaacactt tgactcatat tcatttcctt ctttcttcgt tgaaacgggt taaaatttta
    10381 aagagtgatc gatctgactg cggctgtaga tggagtagag gtgaacctaa gacaggataa
    10441 gtgatgaata aggtggcatg aacaccttga aatcatcaat aagtatccta aaaacgaaca
    10501 agtacaaaaa cattgaaaac gacaataatg aatttttgtt cctcttagta tttctaccga
    10561 ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa ttaagattat tatatgaaaa
    10621 caggttttat ttgtcaattc ataattggtt ttttttaaag caaaagataa tctatatcat
    10681 gatgaacaaa cagagttttt tcctccaatt gatggctttc ctgatctttc ctattttgaa
    10741 taaagtgttt ctgtataatt tttttatttt ataagtaaga taaataatga aagaatagag
    10801 atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca aaaggactgt
    10861 tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga ccgtaataat
    10921 ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga agaatatgga
    10981 gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc ggtagcagcg
    11041 gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt
    11101 tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat aggtactcga
    11161 ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat ttatttaggt
    11221 acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga gcatacattt
    11281 gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta tgaaaaatta
    11341 ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc ggatatttgg
    11401 atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa
    11461 gtaatcagct tcaagaaata agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa
    11521 attccaactt atttccgaag aagctacttt tggaacaccg tgtaccagag gttgtgaaaa
    11581 agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcta catttttgca
    11641 taaatttggc ttaataccga gaaactggct tttgaacacc gtttattcag ataagggact
    11701 gtgacaagac ttttgtcaca gtttcttttt gtttattctt ttttatcttt gtatggcaat
    11761 aatagacaat ataacgaaaa ttatttaatg gcttttggaa gaccatggaa aaccttgtac
    11821 aaagctattt tttctgaaaa attccttgaa acctcacgaa aaaatcatta ttttatgaaa
    11881 aagacaaaaa aaagtgttga ttttttaata attattcatt aaactcttaa acaacttcag
    11941 aaaatattaa ttattctgaa aattccaata aaggggtacg taaaatgaaa aagaaattta
    12001 catttggttt ggttgcggta tgcggccttg tacttgctgg atgttatggc ggcagttcag
    12061 ccacaagcag tagttcagca aaaagcggtt cagcagatgg aggaggcgta tttaacttag
    12121 tcgtgcctca agaaatgcct acagctgact tatcagttgc aacagataca atcagtttta
    12181 cagcattgaa taacgtttat gaaggaattt accgtcttga tgaagacagc aaaccacagc
    12241 cggcaggagc tagcgagttg gcagaagtca gtgaagacgg attaacttac aaaatcaaat
    12301 tacgagaaga cgcaaaatgg tcaaatggtg atcctgttac agcagcggat tacgtttatg
    12361 gctggcaacg aacagtagac ccagctacag cttctgaata tgcttacctg tatgcgccag
    12421 ttgaaaatgc agaagacatt acagctggaa agaaagataa atcagaacta gggatcaaag
    12481 cagttggtga ctatgaatta gaaatcaaat tgacgaaaca aacaccatat ttccaatatc
    12541 tgcttgcttt cccatcattt ttcccacaaa aacaatccgt agtggaagaa aatggggatg
    12601 cctatgcatc tgcaagtgat aaagctgttt ataatggacc attcacatta gaagattttg
    12661 atggaccagg aacagataca gaatggactt ataagaaaaa tgatcattac tgggacaaag
    12721 atacagtaaa actttctgaa atcaaagtca gtgtagtaaa agaatcttct actgcattga
    12781 acttattcaa agatggccaa gcagatgacg tgatcttgtc tggtgagtta gcacagcaaa
    12841 acgcaaacga tccagcttat acttctgtta aagaagcgcg gacaagctat atcgaattca
    12901 atcaacgtga aaagaattca ccattcaaca atgtgaacct aagaaaagct atttcttatt
    12961 ctatcgatcg tgaagcttta gtaaaacaag tactaggtga cggatcagtt gtctctacag
    13021 gattgatccc atcagatatg gtaaaaagtc caacaacgaa cgaagacttt gcaaaagaag
    13081 ctggaaaatt agtttcttat gacaaagata aagcaaaaga atattgggaa aaagcgaaaa
    13141 aagaattggg cgttgattct ttagaatttg atttgatggc ttctgatgat gattcatcga
    13201 aaaaagtaat cgaatatgtt caaaactcaa tccaagaaaa cttggatggc gtaacagtca
    13261 aaccaacacc tgttccattc tctgtacgtc ttgaccgctc aacttcgggt gattttgacg
    13321 cagtactagg cggatgggct gctgactatg cagacccaag cagctttact gatttgtttg
    13381 tcacaggtaa ttcatacaac cgtggtcgct ggagcaatgc agaatatgac gaagcagtta
    13441 aagcagcttc atcaacagac gctggcgatg aacaagcccg ttgggatgat ttccaaaaag
    13501 caatcaagat catctctgat gatatgggtg tcgttccggt gtaccaaaaa gcagaaggtc
    13561 acctagtgaa tgaaaaaatc aaaggtatcg ttcaccatgc ggcaggcgct tcatgggatt
    13621 acaaatggac atacgttgaa gaataatcgt taataccgtg agcatgagaa aaaacgaggc
    13681 tgcggcaaat gtcgcaacct cgttctcata ttcacttatc ctaacaaaga aaaaagcatg
    13741 aaaatagcag aaagagcaat tagtattccg gcaatgatca gccaaaagct gtacataccg
    13801 tagctggcag acgagagcga ggaaaattct ggttttttct ttcggttcct tgttcgtgca
    13861 agatggaccg atcgctgaaa atgatcgaag aaacccgaag aaaagaccca taaaaaaccg
    13921 ccgataatca gaaacggcaa tgaccataag aatagatcat tagataattg aatgagtgta
    13981 aaggaatggt tgatgagatt ttttattaaa ataaccagaa tcagtagaaa agctactgcg
    14041 taggggatag attttttttt catttgattg ctcctttatt tttggttcat tatctaataa
    14101 tgtagcgaaa catctgtcag aagtcaaaag ctagggggaa acattagtga atagttatat
    14161 caaatatgta ttaaaacgtg tctttttcat gatcatcacg ctttggttaa ttgcaacaat
    14221 cacctttttc ttgatgcaat tgctgccggg cacgccatat acgaatcagg aacgtttgag
    14281 tcctgaaaca atcgagatgc taaataaaca agtcggtttg gataaaccag ttatcgtaca
    14341 atatgggatt tatctttcta atttgcttca aggagatttt ggaatttcct tccaatttaa
    14401 aaaccagcca gtcgcacatt tgttagctgg aagaatcgga ccatccttgc agttagggtt
    14461 acaggcaatt atctttggaa cagtcctagg gacgatttta ggaacgatct ctgccatgaa
    14521 gcaaaataca tgggcagata catcttctac actcgtagcg atattaggtc gttccattcc
    14581 aaactttgta tttgctgttt tattacaata tatctttgca atcaaattac aagttttgcc
    14641 aatcgcaaaa tgggatggct ttgtgtacac aatcttacca acaattgcgt tggccatgtc
    14701 gccgcttgct gactctgctc ggtttatccg tacggaaatg gtggaagtac tgcatagtga
    14761 ttatgtggaa ttagcaaaag caaaaggatt gagccgttgg gaaatcgcct ttaaacatgg
    14821 tcttcgaaat agtttgattc cattgatgac tttattagga ccgttagctg tagcattgat
    14881 gaccggatca ctggtggtag aaaatatctt tgctattcct ggtatcgggg aacaattcgt
    14941 taagtcaatc acaacaaatg attatcctac gatcatggct gtaacgatcc tttactcatt
    15001 tatgctgatt tttgttattt tgatcgtgga tctattgtac ggtctagttg atccaagaat
    15061 tcgtgtttca gaagggagcc gaggataaat ggaaatgata ccaaagcgtt atgaaacagt
    15121 tgcagatatc cctgcggatg aatttcttcc attacaaaaa aataccgaag aagaacgtga
    15181 acagattact gcaccttctt tgaattttat ccaagattca tggcgccgtt taaagaaaaa
    15241 caaagctgcc gttatttcaa tggttttact attggtcatc attttgatct ctgtgatcac
    15301 aatctttgtt tctcctcatg atccaactgc ccaaaacgtg gcttatatca atttgcctcc
    15361 tcgtattccg ggaatcaata tcgatggatt gaatggaaaa gcaatggttg gtggtgaact
    15421 tgttgataaa tatgctcaag caaacgtccc ttcaaacgta aatttctttt taggaacaga
    15481 cggtttggga cgtgatgtat taagccgctt atttatggga atgagaatct cgttgctgat
    15541 cgcatttatt gctgcattac tagatgtaac gattggtgta gcttacgggt tggtttctgg
    15601 tttgttaggc ggacgtgtcg ataacgtgat gcaacgtttt ttggaaatct tatcaggaat
    15661 tccaaacctc gttgtcatga ttttgatgtt ggtcgttttt gaaccaggaa ttttttcaat
    15721 cgtggctgct atggccatca caaactggat accaatggca cgtatcgttc gtgcacaaac
    15781 aatgaaatta aaagatcaag aatatgtttt agctggattg acattaggtg aatcaagaat
    15841 gaaaattgca ttcaaacata ttttgccgaa tatctctagt gtcattatta ttcaaatgat
    15901 gtttagtatt ccatctgcta ttttcttcga agccttttta agctttatcg gtttaggtct
    15961 tacgcctccg tcagcttcat taggaacgat gttgagcgaa gggtacaaga cattcctgta
    16021 tctgccttat ctattatgga ttccggcagt aactttatcg gttatcatga ttggctttaa
    16081 cttacttgct gatggattgc gtgatgcctt tgatcctaaa atgaaagagt gaagacgatg
    16141 acgaaaattt tagaagtgaa agacttagaa atctcttttg atacgtatgc tggaaaggta
    16201 cgagcgattc gcggtgtcaa ttttcatttg aataaaggag aaacattggc gatcgttggg
    16261 gaatctggaa gtggaaaatc agtgacgacc agaagcatca tgcgcttgct gtcaagcaac
    16321 gccaatatag atgctggtca aatcctcttt aaaggtaacg atatcgttgg aaaatcagaa
    16381 agagagatgc aaaaaatccg tggtaaagaa atagcgatga ttttccaaga tccaatgacc
    16441 tctttagatc cgacgatgcc cattgggaag caagtcgctg agtcccttag aaagcataat
    16501 aaagtttcaa aaaaagaggg acaaaaagct gcattagaat tgcttaaatt agttggaata
    16561 cccgaagctg agaaacgtat caacagttat ccgcatcagt tctcaggagg tcagcgtcaa
    16621 cggatcgtga tcgcgattgc actggtatgt tatccggaaa tcctgattgc tgatgaacct
    16681 acaacagcgc ttgatgtgac tattcaagca cagattttgg agctgctgaa agaaattcag
    16741 acaaaaatcg atacatcaat tatctttatc actcatgacc ttggtgtagt agcgaatgtc
    16801 gctgatcgtg tcgctgtcat gtatggagga agaatcgttg aagttggaac atcggaagaa
    16861 attttctaca acccgcaaca tccctatact tggggactgc ttggttcaat gccgacatta
    16921 gatagtgcga acgatcgatt gtatgctatt cctggttctc caccggattt attgaacccg
    16981 ccaaaaggag acgcattcta tccaagaaat gaattcgcga tgaaaatcga tgcagaacaa
    17041 gaaccaccat tttttgaact atcaaaaaca cacaaggcag caacttggct acttgctccg
    17101 caggcaccca aagtgacacc gccagaagaa atccaacgtc gctgggctat ctttaaagag
    17161 aaacaaaaac atacttacgg tcaaggagga attgctgatg ccaaataaac tacttgaagt
    17221 taaaggattg aaacagtatt tcaatgtagg aaaaaagaat gaagtacatg ctgtaaatga
    17281 catcagcttt cacatttatg aaggagaaac attcggttta gttggagaat ctggaagcgg
    17341 aaaatccact actggccgta cgatcattcg cttgaatgaa cctactgatg gtgagatatt
    17401 attcgacggt caagatataa cgaagatcaa agataaaaaa ggtttgacga aattccggca
    17461 tgatgttcag atgatctttc aagatccata cgcttcgttg aatcctcgta tgaaagtcag
    17521 agatatcatt gccgaaggaa ttgatgttaa tggactagct aaatcatctg aagagcgtgc
    17581 aaaaaaagtc gatgatctat tgagaactgt tggattgaat ccaagccacg gtactcgtta
    17641 tcctcatgaa ttttcaggtg ggcaacgcca gcgtattggt attgcacgag cattggcagt
    17701 aaaaccgcgt tttatcattt gtgatgaacc aatttctgca ctagatgtgt cgatccaagc
    17761 ccaagtggtc aatttgttgc aagatctcca aaaagaacat caattgacct atctatttat
    17821 tgcacatgat ttgtcgatgg ttaaacatat tagtgaccga atcggtgtaa tgcataacgg
    17881 cttgttatta gagatgggaa caagtgatga aatctataat cacggtgttc atccttatac
    17941 agaaagtttg ttatctgcta ttcctctacc agatccagac catgagagaa aacgcagaag
    18001 aatcaaatat caacctgaac cagatgacgg acaagtacgt caacttcgtg aaatcgcacc
    18061 agaacatttt gtctatgcaa ctgagcagga agtcccatat tacgcaaaaa aactaaaacg
    18121 acaaaaggaa tctcttctgg ttgctaacta ggagggacat agatgctaaa agcttttaaa
    18181 ttccgtatgt atccgacaga agaacaaaaa cagcaattga ttcgtacttt tggatgtgct
    18241 cgatttacct ataaccattt gctgaaaaag cggcagaaaa gttggcaaca aacaggagta
    18301 gccaattttt cgctcacacc cgctacatta aaaaaagaat acccattctt aaaagaagta
    18361 gacagtcttg ctttagcaaa tgcacagctt aatttggatc gggcttttcg caattatttc
    18421 aagggacgtg ccagcttccc aaagcttaaa acgaaaaaaa gtatgtggca atcttatacg
    18481 accaacaacc aaacccgtac gatctatctg gaaaatggct acttaaagtt accaaaacaa
    18541 aaagaactaa tcaagataaa tcagcatcgg ccagttgaag gatcgattcg ctccgctacg
    18601 atttctgctc gatataatga agaattttat gtcgcactac tttgtgatgt ttcgccagta
    18661 aaaaaagaat ctttagctaa atggatcggg atcgcctatc atccgaagac attgatccaa
    18721 acatctcgac ctctagaagt aactttacca aaatttcacc agacagaaga aaagctgcaa
    18781 catgcacagc ggaaattgaa tgtgaaagtc cgctccgctc accatagaaa gatcaggtta
    18841 gatcaggcaa gcaattatca gaaacaaaag agaaaagtaa tggatcttta tctgaaacaa
    18901 aagaaccagc gagaagatta tcttgaacaa ttatctggga aattggtcaa acaatatgac
    18961 tacttatttg tagaatcttt tccaaaagaa gaggcacatg ctgatttttc tattcacgac
    19021 tggcataaat taataacaaa attgcgttat aagtcccaat ggtataataa aaaatttctt
    19081 ttgatcaata ccgacggagc agaagaaagc aattccgtaa gaaagagcca agtagtagaa
    19141 aaaatgggca gacattcggt tatcaaggga taaaaaatga atgaaactga taggagcgtt
    19201 agggttagcc ttgttaataa aaagtcttaa tgacttttgt ttctaggaat ttttcttttg
    19261 aagaaaagct cactaattat gacaaaccaa taaaacaata gcagaaaaag ttcgtacgag
    19321 ctttttctgc tattgttaat tttttattca gaaagtttac gaatacatca aaattctgaa
    19381 acaaaaaacg taactcacaa agagttgttt ttgcgttttt tcttgaatga ttaggtctga
    19441 atcttttgct cacaacctta ttcatggtgc cccggcttga gctgatagat ttttgaagga
    19501 cggccgattc ctacaggacg ttcaccgatc tcatcgaaat attggagcat agctttctta
    19561 aaattggaat gatcgatcgt tttataatcg atccctaaga atttagcaaa tacttttcga
    19621 gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa cttgcggctc gtgttccatt
    19681 ttattgacta cacggttgaa tgctttcaag atgatttgac tatggtcaaa agccaaagta
    19741 tctttgccag aagactctcc agtgactaaa tcaagcgaga tagccacttc tccgctagat
    19801 aaatggatct tgttactatg acgttctagt gtgaaccagc gtacttcctt ggcatcatct
    19861 ccggcgatta gcggttcttc tccaataaag gctaagtagc tgactgtgac gacccagcct
    19921 cgcggatctc gattaggcgt actgaatgta tgcagttgct caatattttc ttttgaaata
    19981 accacaccag tttcttcctt tgtttctctt aagacgcttt ctccagttga ctcatccttt
    20041 tggacaaagc caccaggcaa tgcccatgaa tttcgataag gatgcccttt tctttggatc
    20101 aacaaaactt tcagctgatc ttcttctttg ttatagcaca tcagtacgat atcgacagtc
    20161 aaagagggct tgtcatattc tggacgttcc tgtttttgat accagttaag aaattcttct
    20221 tcacttgctt tatgttcata atataatttt tcttcttctt tagaagcaaa acggttcatc
    20281 tgaccacctc cattgacagt aaaagtactt tttaccttat tgtagcagac tctgaaaaaa
    20341 aaccaaggat ttacttgaaa aatctaggtt atcatttaaa tatgaaaaat aaatggaagt
    20401 atgagattga ataaaaagaa aagcagataa aacggcaata ctttataccg ctcttcacga
    20461 atggcaaaga agccacaaat aacgatatat acgtcaatga taaaacgcaa aatccaagct
    20521 gctgtcataa aactgttcaa atgcccatgg aggttaaaat aaccatacat acgaatataa
    20581 aaatagctga atattaaatg tgctaatgaa agaaaagcaa tactaagtgt aaaaaaaaga
    20641 tgtttcttct gcatgattgt tccagtccct tactaagata gatagtcttt attataccac
    20701 gtgtgagggg agaaataaaa agccgttttt tagtagatat gaaacaaaac aaaaacccgc
    20761 atttattcaa cctattcatc cttttaaagg atagattgaa cgtagataga agtcgtttat
    20821 ccagtctttt tcattttgaa aaaaagctaa cagcatgctt tttttatggt atactagcaa
    20881 ggaaacgaat tcgcatcatg cgataaagga ggaagagccc cgtggcagaa aaggaaactt
    20941 tttatatcac tacgccaatc tattatccaa gcggcaaact tcatatcggc aactcgtata
    21001 ctacgattgc atgtgacgca attgctcgtt ataaacggtt gatgggcttt gatgtgtttt
    21061 atctcactgg agtggatgaa catggtcaaa aaatcgaaac aaaagcagaa gaactaggtg
    21121 taaaaccgca agaatatgta gataaaatgg cggctgatgt caagaaatta tggaaaacgc
    21181 ttgatatcag ttatgataaa ttcattcgca cgacagatga ttaccataaa gcagctgttc
    21241 aaaaaatatt tgatcgttta ttagaacagg gagatattta cttaggtgaa tatgaaggct
    21301 ggtactctgt ttctgacgaa gaattcttta cagaaacaca acttgcagaa gtgtacaaag
    21361 atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg acacgaagta gaattggtaa
    21421 aagaagaatc atacttcttc cgcatgagca aatacgcaga ccgtctattg gaatactatg
    21481 aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa cgaaatgatc aacaacttta
    21541 tcaaaccagg attagaggat cttgcagttt cacgaacaac ttttacttgg ggagtaccag
    21601 tcaagagtga tccgaaacat gtggtgtatg tatggatcga tgcattatcc aattatatta
    21661 ctgcactagg atatggctcc gaagatgaaa gtctattcga aaaatactgg cctgcagatg
    21721 ttcagatggt cggtaaagaa atcgttcgct ttcatacgat ctattggcca atcatgctga
    21781 tggcccttga tttgcctcta cctaaaaaag tatttgggca tggctggcta ttgatgaaag
    21841 acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc tgaaatgctt gtggaacgtt
    21901 atggtttaga tgcactccgt tattatttgc ttcgtgctgt tccgtttggg tcagatggtg
    21961 tctttacgcc agaagatttt gtttcccgtg tcaattatga tctggcaaat gatctaggga
    22021 acttattgaa tcgaacgatt gctatgatca acaaatattg tgatggactc gttcctgact
    22081 atgcctcact tgtcactcca tttgatagtg aactgtctac gacggctgct aacgttgtcg
    22141 gacgttacca tgatgcaatg gaaaaaatgg aattcaatac agcattggct gaaatctggg
    22201 tgctgatttc tcgtgcaaac aaatacattg atgaaacaga accatgggta ttagcaaaag
    22261 acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca tttagctgaa tctttgcgta
    22321 tcgcagcgat attacttcag ccaatcatga cagaaacgcc agagaagatc ttcaatcaac
    22381 taggattgga ctctgaaaca atgaatcttg aaggattaca ctttggcgaa tttccttcag
    22441 gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg tttagatatg gaagaagaag
    22501 ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac aaacgaagat acagtcaaat
    22561 gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa acaaatcaaa tttgatgtat
    22621 ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg tcaaaaagta gaaggggcag
    22681 ataagttgct gcaattccga ctagatgctg gagacagcca agaccgtcaa atcttgtcag
    22741 gaatcgctga attttatcct gatccaagcg aattgatcgg gaaaaaagtc gtcattgtag
    22801 caaatctgaa accacgaaag atgcgtggac aaatcagtca aggaatgatc ctttctgcag
    22861 aagcaccaga tggctcttta caagtaatcg aagcaccaaa atccatgcca aatggctcag
    22921 aaatcgcata gaaattcaaa gggggtatga cactagttgt gtcatacccc ctttttccga
    22981 aatttgagaa gcaattttcg taaattcctt cttattactt gaagccaatc atataataca
    23041 gaaacagaga actatccaaa ataaatccta gaaaaagaag tacgagcttc gctgattttt
    23101 gttaatttag aataatacta ataggaatcg taaaaacaga aaaaaagcat aatgataaag
    23161 ggtcgggcag attttaattg attcatctgg tactcttttt tattgtcctt acattcataa
    23221 agtttgaatg caatgacttt attacatcta tatttatatc taacagtagc gttgatgtgt
    23281 taattataag ttctatcact ttatgttcaa ttagtttgtg ctcttatatg tgtgattgaa
    23341 aatatagata aaaaaacaat catcggcttt gacttcgatg attgttttct ttattttgta
    23401 aaaatcctat cacttgattt tatggtaacc ttttattttt gtcttcaaac ggcttggtaa
    23461 gagagcagta agcagtgcaa atgggatcga aaagacatac cagaaaatcg tacgtccgat
    23521 accataatgt tgatagagct ttgtcaacat cacgtgtaac aagatcaacc ctaaaagcaa
    23581 ggagccagct ataaaccaat gattatccca atgcatgact tcactaagtg ccatcactcc
    23641 aaaaggatag aaataaaaat agatcccgac taaggtcatc cgccagttct ttgtaaatgt
    23701 tccgatattc aataaaaaga aaatcgctga aagcaaaata ccgataggcg gaaaaagccc
    23761 aaccaaaaag gaatacaaca tcccccacaa gatcatcaca ggacctttga tcaatgcaac
    23821 gattgcgatg aacaaagtca gcaatattag tttttgtgat aacgttgcaa cagtcattaa
    23881 tccaaataaa attaatgtga accaatgcca atgggactgc tgttgttctt tacgggaaaa
    23941 atcgacccgg ttggataaat aatctgctaa tttcggataa gaacgcttaa aatccataaa
    24001 ctgttcctcc ttcgtggaaa aggtaggttt agtatacgaa aaataaaaaa gaaatacatc
    24061 cgtcttaaga gggagttttt cactgaaagt ggagaaaaaa gaatggtaaa ttaaaatatt
    24121 ctcagttata ctgaaaatta taccaaattt caaagaagaa agaggatggg aatgaaaggc
    24181 aaactaaaac gagaaatcaa tctgtttggc gcattagcta ccgttatggg aaccgtgatc
    24241 ggtgcaggcg tcttcttcaa aactgctgct gttactgcta gtactcagtc tacaagtttg
    24301 acattgctag cttggctttt gggtggtttc ttaacgatct gtgctggatt gacagtggct
    24361 gaattagcta cagcaatccc ggaaactggt ggagctgtaa aatatattga agctgcgtat
    24421 ggaaaattgc ccagcttttt attaggatgg gcgcaaagct tgatttactt tcctgctaat
    24481 attgctgcat tatcgattat ctttgcgaca caattgacga acttattaca attatctacc
    24541 gattatcttt tgttgattgc agtgatcacc gctgtttctg tgacagggtt gaatttgtta
    24601 gggacaaaag tcggtgcttc tgtacaatca gtgacgttga ttgttaaatt aataccaatt
    24661 gctgtgattg tcatatgggg attattgacc ccaggacaag gaactgtcca gttatttcca
    24721 atcgaagctg gaaaagatgt tacttttgtc gaaggattga gcagtgcgtt gttagcaacg
    24781 ttgtttgctt atgacggctg gttaggtgtc ggtgcaatgg caggggaaat gaaacggccg
    24841 gaaaaagatt tgcctaaagc aattattctt ggtttgagtt ttgtaacagt cgtttatcta
    24901 ttgatcaatt ttgttttttt gaaaactttg ccgattgatc atcttgccgg taatttgaat
    24961 gcagcttctg aagcatcaga cgtcattttt ggaggaatcg gtggaaagct ggtcactatt
    25021 gggatcttga tctcggtata cggtgcattg aacggttata cactaactgg gatccgcgtg
    25081 ccttatgcga tggctttaga agacgagcta cctttcagta aacagctgac aaagctttca
    25141 aagaagttta cggttcctta tgttcctgcg gttttccagt tagcagtagc ttgtattatg
    25201 atgagccttg gttctttcga ctttttaaca gacatgttga tttttgtgat gtggctattc
    25261 agtttactga tttttatcgg tgtctttatt ttaagaaaaa aagcgccgga attgccacgt
    25321 ccatataaag taccacttta tccaatcgtt ccgattattg cgattctcgg tgctattttc
    25381 attttaggta tgacgatgat gacgcaaacg aaattagcat tgatcggtat aggtgttacc
    25441 ttgataggta tcccagtcta ttatcaaaaa aagaaaaaac gctcagaaga gtaaaatatt
    25501 tcaacaacag acagaaaaag tacgaactag gtcctctaga tcttcgtgct ttttctattt
    25561 caataaaaca agttcttcta ttttgatttt tgctacttct gcaagtagtg cttatgtgct
    25621 aaagtaagaa tgaaaaggag agataaacat gatatttgat tctcataccc atttaaatgc
    25681 tgaacagttc aatgaagata ttccagaaac gattcagcga gcacaagaaa tgggggtaac
    25741 gaaaatggcg gtcgtgggct tcgatacgcc aacgattgaa aagtctttgc aattaagcca
    25801 tgactatcca aatatataca gtattatcgg ctggcatccg acagaggctg gcagttatac
    25861 gaaagaaatc gaaaataaat tacaagaaca attgacttta ccaaaggttg tagcattagg
    25921 ggaaattggc ttggattatc attggatgga agacccgaaa gaagtccaag ataaagtatt
    25981 tcgcagacag attgctatcg caaaagaaat gaacctacca atcagtattc atactagaga
    26041 agcacttgaa gacacgtatc aaatcctaaa agaagaagat gttcgagata tcggtggaat
    26101 catgcatagt ttcagtggtg attatgaatg ggcaaaacga tttcttgact tagggatgca
    26161 tatttcattt agcggtgtcg tcactttcaa aaaagcatta gacgtccaag aggctgcagc
    26221 aaatgtacca atcgaacgat tacttgtgga aacagatgcc ccttatctgg caccagttcc
    26281 ttttagagga aagcgaaatg aaccaggcta tactcgttat acagtagaaa agattgctga
    26341 acttcggaat cttccgacag aagaagtggc tgcccaaaca tggacaaatg cgcaccgatt
    26401 atttggtcta agcgaacatg gataagatga agatccaaga gattatagtc gttgaaggaa
    26461 aagacgatac aagaaggctg cgggaagtag ttgatgcaga tacgatagaa acaatagggt
    26521 ctgcaatcaa tgaagagatc ctgatgcaaa ttgaacatgc ccaagagact cgtggagtga
    26581 tcgtttttac cgatcctgat ttttcaggag aaaaaataag aaaaatcatt atggaagctg
    26641 ttcctcaggc aaaacatgct tttttgccgc gcacacaagc taaaccaaag aaaaagggaa
    26701 gccttggtgt cgagcatgca agtgatgctg ctattttaga agcattgaaa aaggtcgtga
    26761 cgccagacat ttcccaaacg gctgtttctg aaatcactag acaagacctt ttagactatg
    26821 gtttaatagc tggggcacaa gcaaaagaaa aacgagaaaa actaggtgac gagttgcgca
    26881 tcggttacac gaacggcaaa caattggaaa aacgtttgaa gatgtttcgt attacgccga
    26941 aagaactcgc ccaagcaatg aaaatagtgg aggaacaaca tgagtgaata tcgtgaaatt
    27001 gcgacaccct ctagaacaaa agagatttta ttgaaacatg gtttttcatt taaaaaaagt
    27061 ttaggacaaa attttctgac tgaaccaaat attttaagga aaatcgtaga aactgcgcaa
    27121 atagatgaac atacgaacgt gatcgaagtt ggaccaggaa tcggtgcatt gacggaacaa
    27181 ttagccaagc atgcaaaaca agtcgtagca tttgagatcg atgatcgttt gatccctgta
    27241 ttggctgata cgatgcaacc ctatgacaat gtgactattg tacatcaaga tattttaaag
    27301 accgatttgt caacagctgt tagagaaaca tttcatgagg agcttccgtt aaaagtcgta
    27361 gcgaatttac cttactatat tacgacaccg atcatgatgc attttcttga atcagatttg
    27421 gttgtggatg aactagtcgt catgatgcaa aaagaagtag ctgatcgtat ctctgctgaa
    27481 ccaggaacga aagcttatgg ttcattatcg attgctgttc aatattatat ggaagccagt
    27541 ttagcattca tcgtaccaaa aacagtattt gtccctcagc caaatgtaga ttcagcgatt
    27601 ttgaaactga caagaagaga tataccggca gtcgaagtga cagacgaaaa agcatttttc
    27661 cgattgacaa aagcggcatt tcagcaaaga agaaaaacgc tttggaataa cttgcagcat
    27721 tcgtatggca aagatgacca gacaaaagca tggttagcaa aaagcttgga aacagctgga
    27781 atcgatccaa aacgtcgtgg ggagactttg tcattacaag aattcgccgc attaagcaat
    27841 gcgatgtcag aaaatatgca gtaaaaaaag aggttgtgac agaagttgtg aaaaagctgt
    27901 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatgtttt tcgtttgttt
    27961 gggcttgtta ccgcagatgc aagcttttga acaccgttta agaaagaggt tgtgggcctg
    28021 ccgtttaact ctaagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttttgagtt
    28081 agttggtctt attacttgat gcaagctttt gaccactatt atgccgaaca aggagatgtg
    28141 acacaaattt tgtcacatct cctatttttg tttcttgatc aaaaaattct tatacttgat
    28201 actcacactc attttgtatg gatttagaaa agaatatgtg tcttgacagt tattagtaag
    28261 tgagttatac taagtcgaca tttatgtgtc aagacatatt aggagagaga aaaaatgaga
    28321 aaaaattcag ttcgaaaact aacgatttct gctttattga ttgggatggg ggtcattatc
    28381 ccaatggtca tgcctaaaat catgattcca ccagcttcct ttactttggc gtctcatgtg
    28441 ccattattta ttgcgatgtt ttttacacca ggagttgcag tggctgttgc gctaggtacg
    28501 acatttggtt tctttttaac aacgccggtt atcatcgcat tgcgagcatt gtcacatctt
    28561 gtgtttgcgt tgattggggc ttggtacttg caaaagcatc cgagtatcgt tttaaaaaat
    28621 ggacagttta cttttgctaa ttggcgtttc caagggttca attttgtgat cggtgtgatt
    28681 cattctttag cagaaatgct agtagtcagc atcttttact ttattggtaa tatgcctgaa
    28741 acatattatt ctcaaggata tttttataca gtattcatct tgatggggat tggtgggctt
    28801 atccacagtt tagttgatta taatattgct tattttatag caggaacact cagcaaatat
    28861 tttgatatcc ccgtatttac ggcagcaaaa aaagaaaatc ggaaagagtc gattcaaggg
    28921 tacgcgaaga aaacgacata ttagacatga tctctcaaaa aaaaaactct catctagtaa
    28981 tggataataa atcctctaag atgagagttt tttgtcctaa aaagagctgc accaacatct
    29041 tctggcacag ctctaacagg gggcaattta tggaagtttg atgacttgtt ctgtttcttt
    29101 atcaaagaag tggcctttgc tgatgttgaa ggcaaggtcg actatttcgc ctggtttatg
    29161 gaaatctctt gcatctactt ttgagatgaa ctcagtagaa ccagtttttg tgtaaagcat
    29221 cgtttcagct ccaagtaatt cggaaacgac aacttctgat ttcactacgg cttcaggtga
    29281 agcttcaagg gctacttgtt cactgtggat gtcttctgga cgaataccga agataacttc
    29341 ttttccttca taaccttttt caactaatac tttgtttttt ccttcaggaa tacgaagctt
    29401 aagcccatgt ccatctgtga taacaccatt tttcaatgtc acattaaaga agttcatggc
    29461 aggagatcca ataaagccgg caacgaacat attgacgggt gtatcataaa cttctttcgg
    29521 tgtaccgatt tgttggataa atccgtcttt catgataacg attcggtcag ccatagtcat
    29581 tgcttctgtc tgatcgtgag taacatagat ggtcgttgtt tcaagacgct ggtgaagttt
    29641 ggcaatctca gcacgcatgg ctacacgtaa tttagcatct aaatttgata atggctcatc
    29701 cattaagaag acttttgcat cccgaacgat tgcccgtccc aatgccacac gttggcgttg
    29761 tccaccagat aaagcagctg gttttcgttt caagtattct gtcagaccta agatctctgc
    29821 tgcattgtcc acacgttttt tgatgtctgc tttatcatat ttacgtaatt tcaagccaaa
    29881 agccatatta tcaaaaacag tcatatgagg atacaatgcg tagttttgga aaaccatggc
    29941 aatatcacgg tctttaggtg ccacgtcatt catgactttt ccgccgatat ttagttctcc
    30001 ttcagagata tcttccagtc ccgcgatcat tcgcaaagta gttgactttc cacaaccgga
    30061 aggaccaaca aaaacgataa attcacggtc tttgattgat agattaaaat cagttacaga
    30121 ataattttct gcattgtcat attttttata tacgtttttc agagccattt ctaccataat
    30181 acgttccaca ctttcttttt tagttttatt aactatttaa agtataaatg aaagcgttaa
    30241 ctaacttcaa tgtcatgttg aacaagaatt ttttcttttt tttgtcaatg tgctagtttt
    30301 aagctttaat aaatatccat aagaaataaa aactgataca ctggagtaaa taagtatgtt
    30361 aacaatatca aaaggagtgt ctcttatgaa aatcgctttg atcgctcatg accgaaaaaa
    30421 acctatgatg gtgaaattag caacagcgta taaaacaata ttggaaaaac atgaactttt
    30481 tgctaccggg acgactgggc agaggatcac ggaagcaacc ggcttgccga tccatcggtt
    30541 taaatccgga ccgttaggcg gggatcagca aattggagcc atgatttcgg aggatcagtt
    30601 agatttggtg atttttcttc gagacccgct ggctgcacag ccccatgaac cagatgtaac
    30661 agcacttatc cgtttatctg atgtctatga gattccgtta gctacgaata tcggaacagc
    30721 tgaggtgcta ctaagaggac tggaagccgg cttcctagat tttcgaagaa tcgtccatga
    30781 aatagacaag catccacttt cattttgatg gataagcgta tggaaaaata acgtttaatg
    30841 ttcgcctata tgtagctata aaagctaaac aaactgataa tatgcgatat aatacgaacg
    30901 tattggataa tataaacaat aatattagtt tttcgtattt gcagtaaatg tgaaaatatg
    30961 ctatagtttc tgtgggaaaa gatcccaaaa ataatacaga gtagaaaata aagcgttaag
    31021 tgctaagagg atgggatgtt gcctcttgga cgaaggacgt aatgttcgcg gttttatttt
    31081 cgcattcgct gcatgatacg atgtagcagc tcttaaagga gatgctaaaa atgaagaaac
    31141 accggttaga cgcacgtatg attgctatta tgggcttatt gattgcattg atggtcactt
    31201 tgtcccgact ggtagcaatc gagaccccat ttattaaaat cagtgtcaca tttattccac
    31261 aagtgatcat gggcattctt tttggtcctt tttggtcagg gataggcgca gttttagctg
    31321 atttagtagg aatggccctc ttctcgaaat ctgcattctt tattggattt acattgaatg
    31381 catttattga aggagcaatc tacggattct ttttctatcg aaaagagatc acttggaaaa
    31441 atgccattct ggctacgctt agtgtcacgc tgatcatcaa cttatttttg acaccattat
    31501 ggcttgcgtt gatgtaccat gtgccattgt tcagctgggt cgtttgggcg ccccgcctgt
    31561 taaaaacagt aatatggtta ccgattcaat caattgccat ttattacgta ggacgtagta
    31621 ttccttacaa aaaaatttta agaagtttag cgatccacgc taaataaaaa acaagcagtc
    31681 ttcccatccc agaagactgc ttgtttttat tgtgctgtga attgtgcttg atagagccgt
    31741 cggtaataac catctttttg aagcagctca tcatgggtgc ccatttcgac aatagctccc
    31801 tgatccatca ctaatatctg gtccgcattt ttgattgtgg acaggcgatg ggcaatcaca
    31861 aaactggttt ttccttccat cattgctaag aaggcttgtt ggattttttt ctctgtcaat
    31921 gtatcgactg agctggtagc ttcatctaag atcaacatag ggggattact gatcatcgtt
    31981 cgagcaatcg tcaacagttg gcgttgtcca tctgatatct ttaacccttg accgccaatc
    32041 ttagtatcta atttttccgg caaacgttct acaaattcat acatataaga ggcttttagg
    32101 gcatcattga tttctgcgtc acttgcttcc ggattcccaa aacgaagatt ttctcgcaat
    32161 gtactgtcaa atagccaagt atcttgtaac accattccaa aacttttacg taatgtatca
    32221 cgttggatag agcggatgtc atggtcatcg actgtaattg ttccttttgt tgtgtcataa
    32281 aaacgcatca gcaaattaac aagtgtggat tttccggccc ctgtttttcc tactatagca
    32341 atggtttgac ctggataagc agtaaaattg aaatcttcga tcaaacgctg cttggggtca
    32401 taagaaaaag aaacatgatc aaaagagact tttcctttga tatttttggg ttccaaggca
    32461 taggcatctg gcaaatcagg cttttcttct ggctgatcga tcaaatcaaa tgctcgttct
    32521 agaccagaaa aagcggtttg cagttgtgtc gtgattccag aaagttcgat aaatggttta
    32581 gaaaactgac ttgagtagat cgtaaagctg gaaatcacac caacagtgat tgacgaacca
    32641 ctcttcaatg ccaataggcc gccaactaaa ccaatcgata aataagcaag gtgatctaca
    32701 aaacgagaag acgggtttgt caaggaagaa gaaaattgtg cccgttgtcc tttttcatat
    32761 agatcagcat ttagcagttc aaattgctgt tgactagctt cttcttgctg aaaagctttc
    32821 acgattttct ggttaccgat tcgttcggtc acaaagcccg agatatcccc aataatcctt
    32881 tgctgtctat cgaaatttgt ttgtgaagca cgagcaacga tccaactcac aaggaaaata
    32941 attggtgtag aacaaagaac gaccaatgtc agaagtggac ttagacgcat cataaaaata
    33001 aacgaaagaa tgatgattgc cactccagaa aataattgat tgaaggccgc agaaattgca
    33061 atggaaatat tgtccatatc atttgtaaat cgactgacga tatttccgtg ggagttttga
    33121 tcataataac gaagtggcaa tcgattcaga tgagcaaatg catcttttct taattttgca
    33181 acggataagt aagctaaccg attgcttaac acttggatga gccattgact gactacgttg
    33241 attaaaacga taccaccgaa tagaataagt aaatggaaca gtcgattgaa gcggacctgt
    33301 ccttctccaa tcatttgatc gatactttga ccaatttgta acgtcatcac aactgttgca
    33361 atcccattga tgattcctag tatcacagct agccaaatgt cttttgcata ggcagtaagg
    33421 taaggaatga atcggcggag cgtctgcaag gataattttt ttactttcat aactattctt
    33481 cctcctgtga tgcgacgatt tcctgatatt ctgctgattg agcaagtaat tcttcatggg
    33541 taccctgagc agcaatctta ccttgatcca tcactaagat atgatgtgct tcttgaatag
    33601 agcggacacg ttgggagata ataatcactg tactgccaag ataagtctgt agcgccttac
    33661 gcaaatccaa gtctgtttga taatccaacg cacttaaaga gtcatctaaa atcagtaatg
    33721 ctggcttggc aatcaaagct cttgcaatcg tcagtcgctg tttttgtccg ccagagaaat
    33781 ttttcccatt ttccatgact tttgtatcta aaccatttgg aagctgacgg acaaattctt
    33841 ctgcctgagc gattttcaga gcttcccaac attctttttc tgtggcgtct ttcttacccc
    33901 attgaaggtt ttcacgaata gtcccggtaa atagtacaga agtttgggga acaagagcaa
    33961 tttgctgacg gagatgatcc agctgccaag aacgaacatc cattccgtcc attataactg
    34021 taccggaact gacatcatag aaacgcggaa tcaattgtgt caaagtcgtt ttcccgcttc
    34081 cggttggacc aatgattcca aggactgtat ttcgcttcag ctgaaatgta atattttgga
    34141 gagaaagacc agaatcagga gtataacgga agtcaacatg gtcaaaagcg ataattgctt
    34201 cagaagaaaa atcgggagta gtactgacct cagcttcaag aatcgaattt tctgtgtcta
    34261 gcacctcttt cacccgattt cctgaagctt cggcacgtgt gaagatcacg acgaggtttg
    34321 aaacaacgat cagtgccaaa agcatttgat tcatataatt gatcaaagca agaacttgtc
    34381 cttgttgcaa gctaccaata tttactttga tcccgccaac tgtcagcaaa cagatgatcc
    34441 cgacattcat gatcaaggtt gtagcaggtg ataatagggc agagatattg gaaacacgta
    34501 aataattgtt cgctagatca tttgtgcttt tatcgaactt ctctgtttct ttttttgtct
    34561 gcgcaaatgc gcggatgaca cgaacaccgc taagattctg actaacgtgt tcattgagct
    34621 gatctagttt ttcttgcact ttttggtaga gaggaacggt ttttttgatg ataaaaaata
    34681 aaatgattga gaaaataggc aataatgcta agaaaaacag acctacttcc cagtcgatca
    34741 caaaagccat gaccactgac ccgatactta aaaacggtgc tctgatcaat aaacgaatga
    34801 ccattgctaa agctagctga agctgattga tgtcgtttgt cattcgggtg atcaaggtat
    34861 ctgttccaaa atgattcaac tcgttgtgag agaaagtatt gatttttttg attagctgat
    34921 tacgtaactc tgtaccaaat ccttgtgaag ctactgaagc atagtattgg caaatgatag
    34981 cacaaatcag accaatcaca gacatagcga ccatccataa gcccatttca atgatttttc
    35041 ctcggtcatt catttttagt ccattatcga tcaaggaagc catcattaaa ggcaggacaa
    35101 gttcgaagac agcttcaaga aatttgaaga acgggcccaa aatgatttgc ttacgatagt
    35161 cttttgcata acgtaataac tgaatcatat gattcctcc
//
LOCUS       DACDKL010000011        34724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDKL010000011 DACDKL010000000
VERSION     DACDKL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.011"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..399)
                     /locus_tag="IV329_000423"
     CDS             complement(70..399)
                     /locus_tag="IV329_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8939631.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            576..1304
                     /gene="sfsA"
                     /locus_tag="IV329_000424"
     CDS             576..1304
                     /gene="sfsA"
                     /locus_tag="IV329_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8939632.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1320..2333
                     /locus_tag="IV329_000425"
     CDS             1320..2333
                     /locus_tag="IV329_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8939633.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2333..3610
                     /locus_tag="IV329_000426"
     CDS             2333..3610
                     /locus_tag="IV329_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8939634.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3673..6375
                     /locus_tag="IV329_000427"
     CDS             3673..6375
                     /locus_tag="IV329_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8939635.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6527..6844
                     /locus_tag="IV329_000428"
     CDS             6527..6844
                     /locus_tag="IV329_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8939636.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6874..7194
                     /locus_tag="IV329_000429"
     CDS             6874..7194
                     /locus_tag="IV329_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8939637.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7302..8762
                     /locus_tag="IV329_000430"
     CDS             7302..8762
                     /locus_tag="IV329_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8939638.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8830..9051
                     /locus_tag="IV329_000431"
     CDS             8830..9051
                     /locus_tag="IV329_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939639.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9082..9264
                     /locus_tag="IV329_000432"
     CDS             9082..9264
                     /locus_tag="IV329_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8939640.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9264..9677
                     /locus_tag="IV329_000433"
     CDS             9264..9677
                     /locus_tag="IV329_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8939641.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9800..10981
                     /locus_tag="IV329_000434"
     CDS             9800..10981
                     /locus_tag="IV329_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939642.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            11370..11443
                     /locus_tag="IV329_000435"
     tRNA            11370..11443
                     /locus_tag="IV329_000435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11404..11406,aa:Arg,seq:cct)
     gene            complement(11480..11680)
                     /locus_tag="IV329_000436"
     CDS             complement(11480..11680)
                     /locus_tag="IV329_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8939643.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(11985..13217)
                     /locus_tag="IV329_000437"
     CDS             complement(11985..13217)
                     /locus_tag="IV329_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8939644.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            13471..14040
                     /locus_tag="IV329_000438"
     CDS             13471..14040
                     /locus_tag="IV329_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8939645.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(14218..14658)
                     /locus_tag="IV329_000439"
     CDS             complement(14218..14658)
                     /locus_tag="IV329_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8939646.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            14816..15580
                     /gene="map"
                     /locus_tag="IV329_000440"
     CDS             14816..15580
                     /gene="map"
                     /locus_tag="IV329_000440"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8939647.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            15612..16535
                     /locus_tag="IV329_000441"
     CDS             15612..16535
                     /locus_tag="IV329_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8939648.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            16611..17750
                     /locus_tag="IV329_000442"
     CDS             16611..17750
                     /locus_tag="IV329_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8939649.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            17743..18543
                     /locus_tag="IV329_000443"
     CDS             17743..18543
                     /locus_tag="IV329_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8939650.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            18543..19370
                     /locus_tag="IV329_000444"
     CDS             18543..19370
                     /locus_tag="IV329_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8939651.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            19348..20082
                     /locus_tag="IV329_000445"
     CDS             19348..20082
                     /locus_tag="IV329_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8939652.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            20182..21048
                     /gene="rfbA"
                     /locus_tag="IV329_000446"
     CDS             20182..21048
                     /gene="rfbA"
                     /locus_tag="IV329_000446"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8939653.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            21062..21634
                     /gene="rfbC"
                     /locus_tag="IV329_000447"
     CDS             21062..21634
                     /gene="rfbC"
                     /locus_tag="IV329_000447"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8939654.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            21656..22684
                     /gene="rfbB"
                     /locus_tag="IV329_000448"
     CDS             21656..22684
                     /gene="rfbB"
                     /locus_tag="IV329_000448"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8939655.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            22782..23633
                     /gene="rfbD"
                     /locus_tag="IV329_000449"
     CDS             22782..23633
                     /gene="rfbD"
                     /locus_tag="IV329_000449"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8939656.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            23668..25701
                     /locus_tag="IV329_000450"
     CDS             23668..25701
                     /locus_tag="IV329_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939657.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            25745..27025
                     /locus_tag="IV329_000451"
     CDS             25745..27025
                     /locus_tag="IV329_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8939658.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            27235..28041
                     /locus_tag="IV329_000452"
     CDS             27235..28041
                     /locus_tag="IV329_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939659.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            28053..29273
                     /locus_tag="IV329_000453"
     CDS             28053..29273
                     /locus_tag="IV329_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939660.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            29263..30849
                     /locus_tag="IV329_000454"
     CDS             29263..30849
                     /locus_tag="IV329_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8939661.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            30888..33026
                     /locus_tag="IV329_000455"
     CDS             30888..33026
                     /locus_tag="IV329_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8939662.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(33394..34377)
                     /locus_tag="IV329_000456"
     CDS             complement(33394..34377)
                     /locus_tag="IV329_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8939663.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT        11078 a         5906 c         7213 g        10527 t
ORIGIN      
        1 aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata tatatacatt
       61 gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag
      121 agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg
      181 atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta
      241 atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg
      301 atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact
      361 tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact
      421 cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat
      481 aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat
      541 gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag
      601 ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa
      661 taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg
      721 aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg
      781 taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg
      841 atggtatttt agacggcacg atccttttac caggattaac tggtccattt acggtataca
      901 agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac
      961 aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc
     1021 ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag
     1081 atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga
     1141 tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag
     1201 ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca
     1261 ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa
     1321 tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt
     1381 taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg
     1441 aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca
     1501 atattactca tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag
     1561 ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc
     1621 caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag
     1681 cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag
     1741 acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat
     1801 tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt
     1861 taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt
     1921 atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg
     1981 agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga
     2041 tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat
     2101 ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa
     2161 tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg
     2221 gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt
     2281 taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga
     2341 atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc
     2401 accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc
     2461 ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt
     2521 ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca
     2581 agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt
     2641 gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat
     2701 tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt
     2761 gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat
     2821 tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc
     2881 agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt
     2941 gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg
     3001 aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt
     3061 ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc
     3121 ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt
     3181 caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat
     3241 tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca
     3301 tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga
     3361 taataatcta agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat
     3421 ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc
     3481 agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa
     3541 agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag
     3601 gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag
     3661 aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa
     3721 caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc
     3781 cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc
     3841 gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg
     3901 caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag
     3961 gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact
     4021 ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg
     4081 ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca aaatccgcaa
     4141 ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat
     4201 agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt
     4261 ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg
     4321 cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa
     4381 tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg
     4441 aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa
     4501 gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg
     4561 ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca
     4621 aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa
     4681 ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac
     4741 cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc
     4801 tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact
     4861 tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg
     4921 gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt
     4981 ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta
     5041 gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg
     5101 gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat
     5161 tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa
     5221 gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga
     5281 gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag
     5341 actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct
     5401 tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc
     5461 aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta
     5521 aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg
     5581 acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa
     5641 gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg
     5701 gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa
     5761 ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa
     5821 ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg
     5881 gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca
     5941 tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct
     6001 gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc
     6061 ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat
     6121 ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg
     6181 ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca
     6241 aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa
     6301 tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat
     6361 aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac
     6421 tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc
     6481 ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac
     6541 aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa
     6601 agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga
     6661 taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat
     6721 gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat
     6781 gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa
     6841 ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc
     6901 atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg
     6961 gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca
     7021 cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat
     7081 atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt
     7141 actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa
     7201 cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga
     7261 gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt
     7321 ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg
     7381 ctttgcgtga tgcttttatc ggcacactgc ccgcaacaat ggcgggatca gtcgctgtaa
     7441 tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt
     7501 tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg
     7561 tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc
     7621 ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga
     7681 ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa
     7741 atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt
     7801 cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag
     7861 ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta
     7921 cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac
     7981 cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg
     8041 atgggcaact gatcattgat ttggtgcaaa aatatattgc agagccattc ctaggattat
     8101 ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg
     8161 gtattcatgg accaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac
     8221 taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt
     8281 tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg
     8341 catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta
     8401 aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca
     8461 acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct
     8521 tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg
     8581 ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa
     8641 caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga
     8701 tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat
     8761 aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttcgagga
     8821 ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta
     8881 gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg
     8941 ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc
     9001 aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt
     9061 taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct
     9121 ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc
     9181 acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa
     9241 gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt
     9301 ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat
     9361 cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc
     9421 gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga
     9481 caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg
     9541 aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc
     9601 ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga
     9661 tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta
     9721 agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt
     9781 tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga
     9841 ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga
     9901 acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg
     9961 gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct
    10021 aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa
    10081 tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct
    10141 ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat
    10201 cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa
    10261 gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga
    10321 aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa
    10381 aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt
    10441 ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc
    10501 tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata
    10561 ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt
    10621 aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta
    10681 tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa
    10741 atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt
    10801 agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt
    10861 agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata
    10921 tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg
    10981 ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt
    11041 gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt
    11101 gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt
    11161 tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga
    11221 aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg
    11281 aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac
    11341 aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg
    11401 cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtacaaat aacccctgta
    11461 tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct aaactgctat
    11521 gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat
    11581 gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa ttgattacta
    11641 aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag attcgaatta
    11701 aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt tcttaactct
    11761 agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga tgacttttta
    11821 ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa agtaccatat
    11881 aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct tagatccttt
    11941 attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca
    12001 tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc catcttgtca
    12061 gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc tgctaattct
    12121 tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc tagtgctaag
    12181 tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga ttgagaaata
    12241 ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc atctgtatca
    12301 agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac ttttccatct
    12361 ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg ctttgtactt
    12421 gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc cgtcggcata
    12481 tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca aagatggttt
    12541 ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc agaaaattgt
    12601 tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt ccatgctaag
    12661 acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa tgcctcaact
    12721 tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc agccgcagcc
    12781 acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat tagtgctttg
    12841 ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa tgcatcggga
    12901 tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc cgcttgatcg
    12961 attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag aaattctctt
    13021 tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt ataagcttct
    13081 tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt
    13141 cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt tagaagttca
    13201 ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta actattagca
    13261 tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg aggttatttt
    13321 tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt gaagaaaatc
    13381 aaaaactaat tttgcatatc aaaaaaacat gttacaatga aattaatcaa tggtggaagc
    13441 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
    13501 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
    13561 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
    13621 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
    13681 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
    13741 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
    13801 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
    13861 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
    13921 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
    13981 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
    14041 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
    14101 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
    14161 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
    14221 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
    14281 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
    14341 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
    14401 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
    14461 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
    14521 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
    14581 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
    14641 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
    14701 taccaaaaca tcattgaaaa gaaaataaaa cctaaagaca cctggggaat gtcatttggc
    14761 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
    14821 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
    14881 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
    14941 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
    15001 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
    15061 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
    15121 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
    15181 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
    15241 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
    15301 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
    15361 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
    15421 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
    15481 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
    15541 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
    15601 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
    15661 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
    15721 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
    15781 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
    15841 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
    15901 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
    15961 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
    16021 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
    16081 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
    16141 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
    16201 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
    16261 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
    16321 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
    16381 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
    16441 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
    16501 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
    16561 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
    16621 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
    16681 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
    16741 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
    16801 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
    16861 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
    16921 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
    16981 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
    17041 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
    17101 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
    17161 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
    17221 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
    17281 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
    17341 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
    17401 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
    17461 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
    17521 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcgtat
    17581 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
    17641 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
    17701 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
    17761 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
    17821 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
    17881 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
    17941 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
    18001 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
    18061 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
    18121 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
    18181 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
    18241 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
    18301 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
    18361 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
    18421 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
    18481 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
    18541 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
    18601 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
    18661 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
    18721 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
    18781 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
    18841 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
    18901 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
    18961 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
    19021 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
    19081 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
    19141 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
    19201 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
    19261 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
    19321 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
    19381 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
    19441 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
    19501 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
    19561 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
    19621 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
    19681 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
    19741 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
    19801 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
    19861 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
    19921 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
    19981 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
    20041 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
    20101 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
    20161 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
    20221 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
    20281 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
    20341 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
    20401 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
    20461 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
    20521 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
    20581 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
    20641 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
    20701 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
    20761 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
    20821 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
    20881 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
    20941 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
    21001 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
    21061 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
    21121 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
    21181 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
    21241 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
    21301 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
    21361 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
    21421 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
    21481 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
    21541 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
    21601 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
    21661 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
    21721 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
    21781 agagaacttg gccggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
    21841 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    21901 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    21961 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    22021 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    22081 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    22141 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    22201 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    22261 agtaacgagt atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    22321 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    22381 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    22441 attgatctta gaaatgatgg gccaaccaaa agacgcatac gaccatgtaa acgaccgcgc
    22501 agggcacgat ttgcgttatg caatcgattc gacaaaattg cgagaagagc taggttggca
    22561 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    22621 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    22681 ataatacaga ggaaatttca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    22741 tgtctctttt ttaaaataaa aatcaaggag cggataacca tatgttttta ttaactggag
    22801 gaaatggaca attaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgccatatg
    22861 tttcaacaga tgcaaaagac ttggatatca cagatgctga gaaaacaatg gcttatatta
    22921 cagaattaaa acccgatgtt attttccatt gcgcagctta cacagcagta gataaagcag
    22981 aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga tggcacaaga aatgtagcac
    23041 ttgcagcaaa agctgtgaat gcacaattcg tctatatcag tacagactat gttttcgatg
    23101 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    23161 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    23221 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    23281 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    23341 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    23401 atcatttgtc gaacgaaaat agctgttctt ggtatgagtt cgcaaaagaa atattgaaag
    23461 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    23521 cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt aggttttgtc ataccaactt
    23581 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    23641 tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa
    23701 gaaaagccaa aagcgaatca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc
    23761 cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt
    23821 tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg
    23881 atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac
    23941 ctctcacaag ctgaagagga cttttcgttg gtgaacgagg attattcatt aaacggacaa
    24001 aacatgatca ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc
    24061 aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa
    24121 ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa gaggaaaaaa
    24181 gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc
    24241 atgcaacatg tgggcgattt gattttcttt actttgggac tgatgattgc tttttatcat
    24301 tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt
    24361 ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg
    24421 ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg
    24481 ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc
    24541 tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg
    24601 gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa
    24661 attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt
    24721 tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa
    24781 aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt
    24841 gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc
    24901 atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg
    24961 agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct
    25021 tatttctatg cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc
    25081 gcagttgctg tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg
    25141 ttttacctcg cttttgtcgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta
    25201 gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa
    25261 caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg
    25321 ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc
    25381 gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc
    25441 gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat
    25501 tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat
    25561 tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat
    25621 atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa
    25681 aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat tagtagaaag
    25741 gagagtgaag ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt
    25801 cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcaggggtag gttttccagc
    25861 aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct
    25921 cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc
    25981 ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt
    26041 actgtttcct ttggttattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg
    26101 ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt tatatcgtgt
    26161 atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc
    26221 tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg ctttattgat
    26281 cagtggattt gccagttctt ttgaacgatg gtatgttcga atcatcggtg ggatagcagt
    26341 cgttgcagcc tttttaggta tttgggtctg ccaatctaga ggtgcattag tcgcgttgat
    26401 tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt
    26461 tattgcctac acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc
    26521 tgaaaaagtc aatttgttta ccgatcgaga agatatttgg cataaattct atcagacgct
    26581 aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc agcgaggaaa
    26641 ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg
    26701 atttgggatt gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga
    26761 tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt
    26821 tatggaggat acattgactt catacccatg ggttccgatt atttatatcc tgttggctat
    26881 ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc
    26941 atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc
    27001 ttccaggatc caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt
    27061 cgcagccttt tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga
    27121 gaaaatcttg tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat
    27181 atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt
    27241 aaagatttgt atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt
    27301 tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt
    27361 atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca
    27421 caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg
    27481 ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta
    27541 gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg
    27601 tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat
    27661 ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca
    27721 ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt
    27781 acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt
    27841 gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat
    27901 cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc
    27961 tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca
    28021 catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag
    28081 atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca
    28141 atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa
    28201 taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt
    28261 tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa
    28321 tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata
    28381 tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg
    28441 atatcattga atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa
    28501 gcgggatgtt cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga
    28561 ttgtggatga agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat
    28621 ttacagaaat caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga
    28681 ttcgtcgttt ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg
    28741 atacgatgga agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag
    28801 tggatcggaa taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga
    28861 aagcggaatt gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac
    28921 tatacgttaa agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa
    28981 ttgcgctacg aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta
    29041 tcaagattcc ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt
    29101 tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt
    29161 tggtatataa gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca
    29221 tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa
    29281 atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt
    29341 aaagttttag agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa
    29401 caatgtgaag tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt
    29461 gctcaagatg gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga
    29521 cagacatttg attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa
    29581 acattgaaga aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct
    29641 aacttggcgc ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct
    29701 tatggtctcc tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt
    29761 tttgaaaaag cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat
    29821 acagaattaa aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg
    29881 cgaccatttg gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa
    29941 agttccattg cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa
    30001 acgaaacaag atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag
    30061 acactaagtt tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa
    30121 cctagcttta tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg
    30181 aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag
    30241 ttcgttctaa cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc
    30301 ggggaattga caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc
    30361 acaaagcagc tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag
    30421 gaaaatacaa gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg
    30481 gaaagtgatg aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa
    30541 aaaatccaag aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca
    30601 aaaatattaa aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat
    30661 aagttatcag ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc
    30721 aatcaagcag aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc
    30781 gagcaagaac gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt
    30841 gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag
    30901 aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg
    30961 tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa
    31021 atgtgattgc tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg
    31081 atgttaagac ccaaagcggt ttcaaggtac gtttatcagg gaaaatgaga ggaaaagcca
    31141 tcttagactt ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat
    31201 acccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag
    31261 ggataaatta tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat
    31321 tgaagccagg atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga
    31381 aagagcaaag gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc
    31441 ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa
    31501 cgtatgatca ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa
    31561 agtgtctgga atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa
    31621 atggacacat cagtctggca actaattctg cattggaaat agcagaagga gaatttatag
    31681 cactcttaga taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc
    31741 taaacgtaca tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg
    31801 gcaatcgctt tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata
    31861 attatatctc tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc
    31921 gtaaaggcta tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc
    31981 ctgcagatca tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat
    32041 caactgcaag taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga
    32101 cagatgccct gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat
    32161 tttatgagat tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga
    32221 agaatggcta tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt
    32281 caaattatga aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat
    32341 ttgcagaata caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat
    32401 tcaattattc tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat
    32461 tcttgaataa cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg
    32521 cacagtttga tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc
    32581 agcatgcagg agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg
    32641 atcgaacaca ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca
    32701 ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa
    32761 cattagaagt agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt
    32821 acaatgtcta tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg
    32881 aagatactcc agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat
    32941 ggccagcata tattgcgcat gatccatttt acaatgataa tttaacaaaa gacggaatcg
    33001 gcgacttttc gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct
    33061 cgcacataac ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa
    33121 aaataagtcg aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa
    33181 aattgctttt caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc
    33241 accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct
    33301 tattcatgct cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc
    33361 tgtcaaagaa tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta
    33421 ttcctctaca aaagccactc tatcaattat catgggttta aaataagaag tgatggagat
    33481 aaccagattg ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata
    33541 tatttcgttc tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc
    33601 ttctaaagca ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg
    33661 ttttccttta tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc
    33721 cgataaacac aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt
    33781 ggttttagga gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata
    33841 attgacatgg tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt
    33901 tgttagctta cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata
    33961 gttgaattcg ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca
    34021 atcttcactt gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat
    34081 tgttagcaag tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata
    34141 ctctggaaaa taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc
    34201 aatgccaatc agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc
    34261 ttttttataa ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat
    34321 ttgacatata tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag
    34381 atattacctc actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg
    34441 attcttcacg taatacaggt attcttatgg actagacagc gctttgggaa gatggattct
    34501 ctgaaaaata taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt
    34561 ctttaattta ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct
    34621 tgtcttgatt agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt
    34681 gcttataaac taggttttta aaggtgacta tggtaagata atag
//
LOCUS       DACDKL010000012        31843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDKL010000012 DACDKL010000000
VERSION     DACDKL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.012"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..2305)
                     /locus_tag="IV329_000457"
     CDS             complement(152..2305)
                     /locus_tag="IV329_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8939664.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="IV329_000458"
     CDS             complement(2599..3447)
                     /locus_tag="IV329_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8939665.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IV329_000459"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IV329_000459"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8939666.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="IV329_000460"
     CDS             complement(5948..6127)
                     /locus_tag="IV329_000460"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8939667.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IV329_000461"
     CDS             complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IV329_000461"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8939668.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /locus_tag="IV329_000462"
     CDS             8275..10554
                     /locus_tag="IV329_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8939669.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10647..11294)
                     /locus_tag="IV329_000463"
     CDS             complement(10647..11294)
                     /locus_tag="IV329_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939670.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11473..12192)
                     /locus_tag="IV329_000464"
     CDS             complement(11473..12192)
                     /locus_tag="IV329_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8939671.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12323..13474)
                     /locus_tag="IV329_000465"
     CDS             complement(12323..13474)
                     /locus_tag="IV329_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8939672.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13691..14431)
                     /locus_tag="IV329_000466"
     CDS             complement(13691..14431)
                     /locus_tag="IV329_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8939673.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IV329_000467"
     CDS             complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IV329_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8939674.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14815..15408)
                     /locus_tag="IV329_000468"
     CDS             complement(14815..15408)
                     /locus_tag="IV329_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8939675.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15392..16039)
                     /locus_tag="IV329_000469"
     CDS             complement(15392..16039)
                     /locus_tag="IV329_000469"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8939676.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IV329_000470"
     CDS             complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IV329_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8939677.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IV329_000471"
     CDS             complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IV329_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8939678.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17485..18012)
                     /locus_tag="IV329_000472"
     CDS             complement(17485..18012)
                     /locus_tag="IV329_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8939679.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18192..18977)
                     /locus_tag="IV329_000473"
     CDS             complement(18192..18977)
                     /locus_tag="IV329_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8939680.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18970..19839)
                     /locus_tag="IV329_000474"
     CDS             complement(18970..19839)
                     /locus_tag="IV329_000474"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8939681.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19840..21219)
                     /locus_tag="IV329_000475"
     CDS             complement(19840..21219)
                     /locus_tag="IV329_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8939682.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IV329_000476"
     CDS             complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IV329_000476"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8939683.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IV329_000477"
     CDS             complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IV329_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8939684.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IV329_000478"
     CDS             complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IV329_000478"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8939685.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IV329_000479"
     CDS             complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IV329_000479"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8939686.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IV329_000480"
     CDS             complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IV329_000480"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8939687.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25177..25401)
                     /locus_tag="IV329_000481"
     CDS             complement(25177..25401)
                     /locus_tag="IV329_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8939688.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25454..26416)
                     /locus_tag="IV329_000482"
     CDS             complement(25454..26416)
                     /locus_tag="IV329_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8939689.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26426..26866)
                     /locus_tag="IV329_000483"
     CDS             complement(26426..26866)
                     /locus_tag="IV329_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8939690.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27172..28137
                     /locus_tag="IV329_000484"
     CDS             27172..28137
                     /locus_tag="IV329_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8939691.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28194..28424)
                     /locus_tag="IV329_000485"
     CDS             complement(28194..28424)
                     /locus_tag="IV329_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8939692.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28510..29397)
                     /locus_tag="IV329_000486"
     CDS             complement(28510..29397)
                     /locus_tag="IV329_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8939693.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29486..30709)
                     /locus_tag="IV329_000487"
     CDS             complement(29486..30709)
                     /locus_tag="IV329_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8939694.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30726..31805)
                     /locus_tag="IV329_000488"
     CDS             complement(30726..31805)
                     /locus_tag="IV329_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8939695.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6971 c         5488 g        10590 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
    10681 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
    10741 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
    10801 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
    10861 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
    10921 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
    10981 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
    11041 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
    11101 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
    11161 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
    11221 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
    11281 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
    11341 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
    11401 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
    11461 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
    11521 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
    11581 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
    11641 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
    11701 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
    11761 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
    11821 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
    11881 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
    11941 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
    12001 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
    12061 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
    12121 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
    12181 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
    12241 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
    12301 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
    12361 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
    12421 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    12481 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    12541 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    12601 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    12661 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    12721 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    12781 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    12841 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    12901 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    12961 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    13021 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    13081 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    13141 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    13201 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    13261 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    13321 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    13381 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    13441 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    13501 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    13561 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    13621 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    13681 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    13741 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    13801 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    13861 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    13921 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    13981 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    14041 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    14101 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    14161 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    14221 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    14281 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    14341 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    14401 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    14461 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    14521 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    14581 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    14641 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    14701 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    14761 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    14821 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    14881 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    14941 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    15001 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    15061 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    15121 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    15181 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    15241 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    15301 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    15361 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    15421 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    15481 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    15541 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    15601 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    15661 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    15721 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    15781 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    15841 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    15901 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    15961 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    16021 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    16081 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    16141 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    16201 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    16261 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    16321 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    16381 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    16441 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    16501 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    16561 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    16621 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    16681 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    16741 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    16801 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    16861 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    16921 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    16981 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    17041 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    17101 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    17161 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    17221 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    17281 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    17341 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    17401 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    17461 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    17521 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    17581 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    17641 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    17701 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    17761 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    17821 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    17881 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    17941 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    18001 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    18061 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    18121 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    18181 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    18241 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    18301 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    18361 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    18421 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    18481 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    18541 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    18601 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    18661 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    18721 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    18781 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    18841 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    18901 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    18961 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    19021 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    19081 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    19141 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    19201 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    19261 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    19321 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    19381 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    19441 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    19501 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    19561 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    19621 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    19681 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    19741 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    19801 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    19861 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    19921 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    19981 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    20041 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    20101 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    20161 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    20221 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    20281 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    20341 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    20401 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    20461 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    20521 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    20581 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    20641 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    20701 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    20761 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    20821 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    20881 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    20941 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    21001 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    21061 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    21121 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    21181 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    21241 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    21301 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    21361 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    21421 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    21481 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    21541 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    21601 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    21661 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    21721 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    21781 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    21841 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    21901 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    21961 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    22021 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    22081 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    22141 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    22201 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    22261 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    22321 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    22381 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    22441 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    22501 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    22561 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    22621 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    22681 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    22741 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    22801 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    22861 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    22921 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    22981 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    23041 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    23101 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    23161 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    23221 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    23281 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    23341 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    23401 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    23461 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    23521 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    23581 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    23641 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    23701 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    23761 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    23821 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    23881 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    23941 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    24001 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    24061 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    24121 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    24181 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    24241 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    24301 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    24361 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    24421 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    24481 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    24541 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    24601 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    24661 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    24721 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    24781 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    24841 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    24901 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    24961 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    25021 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    25081 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    25141 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    25201 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    25261 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    25321 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    25381 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    25441 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    25501 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    25561 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    25621 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    25681 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    25741 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    25801 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    25861 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    25921 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    25981 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    26041 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    26101 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    26161 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    26221 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    26281 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    26341 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    26401 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    26461 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    26521 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    26581 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    26641 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    26701 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    26761 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    26821 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    26881 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    26941 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    27001 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    27061 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    27121 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    27181 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    27241 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    27301 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    27361 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    27421 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    27481 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    27541 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    27601 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    27661 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    27721 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    27781 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    27841 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    27901 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    27961 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    28021 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    28081 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    28141 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    28201 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    28261 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    28321 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    28381 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    28441 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    28501 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    28561 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    28621 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    28681 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    28741 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    28801 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    28861 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    28921 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    28981 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    29041 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    29101 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    29161 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    29221 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    29281 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    29341 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    29401 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    29461 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    29521 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    29581 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    29641 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    29701 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    29761 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    29821 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    29881 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    29941 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    30001 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    30061 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    30121 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    30181 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    30241 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    30301 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    30361 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    30421 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    30481 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    30541 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    30601 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    30661 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    30721 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    30781 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    30841 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    30901 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    30961 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    31021 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    31081 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    31141 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    31201 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
    31261 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    31321 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    31381 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    31441 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    31501 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    31561 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    31621 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    31681 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    31741 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    31801 aacatagcct acaaactgtc actcaaactt aagccctatt ttt
//
LOCUS       DACDKL010000013        30160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDKL010000013 DACDKL010000000
VERSION     DACDKL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.013"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(128..817)
                     /locus_tag="IV329_000489"
     CDS             complement(128..817)
                     /locus_tag="IV329_000489"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8939696.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(824..1540)
                     /gene="pyrF"
                     /locus_tag="IV329_000490"
     CDS             complement(824..1540)
                     /gene="pyrF"
                     /locus_tag="IV329_000490"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8939697.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1537..2469)
                     /locus_tag="IV329_000491"
     CDS             complement(1537..2469)
                     /locus_tag="IV329_000491"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8939698.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2466..3242)
                     /locus_tag="IV329_000492"
     CDS             complement(2466..3242)
                     /locus_tag="IV329_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8939699.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3296..6481)
                     /gene="carB"
                     /locus_tag="IV329_000493"
     CDS             complement(3296..6481)
                     /gene="carB"
                     /locus_tag="IV329_000493"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8939700.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6486..7562)
                     /gene="carA"
                     /locus_tag="IV329_000494"
     CDS             complement(6486..7562)
                     /gene="carA"
                     /locus_tag="IV329_000494"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8939701.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7567..8847)
                     /locus_tag="IV329_000495"
     CDS             complement(7567..8847)
                     /locus_tag="IV329_000495"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8939702.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8866..9792)
                     /locus_tag="IV329_000496"
     CDS             complement(8866..9792)
                     /locus_tag="IV329_000496"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8939703.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9805..11088)
                     /locus_tag="IV329_000497"
     CDS             complement(9805..11088)
                     /locus_tag="IV329_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8939704.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11102..11641)
                     /gene="pyrR"
                     /locus_tag="IV329_000498"
     CDS             complement(11102..11641)
                     /gene="pyrR"
                     /locus_tag="IV329_000498"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8939705.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11876..12787)
                     /locus_tag="IV329_000499"
     CDS             complement(11876..12787)
                     /locus_tag="IV329_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8939706.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12784..13248)
                     /locus_tag="IV329_000500"
     CDS             complement(12784..13248)
                     /locus_tag="IV329_000500"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8939707.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13253..13951)
                     /locus_tag="IV329_000501"
     CDS             complement(13253..13951)
                     /locus_tag="IV329_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8939708.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13938..14324)
                     /locus_tag="IV329_000502"
     CDS             complement(13938..14324)
                     /locus_tag="IV329_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939709.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14471..16141)
                     /locus_tag="IV329_000503"
     CDS             complement(14471..16141)
                     /locus_tag="IV329_000503"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8939710.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16324..16557
                     /locus_tag="IV329_000504"
     CDS             16324..16557
                     /locus_tag="IV329_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8939711.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16656..17087)
                     /locus_tag="IV329_000505"
     CDS             complement(16656..17087)
                     /locus_tag="IV329_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8939712.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17166..17579
                     /locus_tag="IV329_000506"
     CDS             17166..17579
                     /locus_tag="IV329_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8939713.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17617..18765)
                     /locus_tag="IV329_000507"
     CDS             complement(17617..18765)
                     /locus_tag="IV329_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8939714.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18887..19483)
                     /locus_tag="IV329_000508"
     CDS             complement(18887..19483)
                     /locus_tag="IV329_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8939715.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19683..22325)
                     /gene="alaS"
                     /locus_tag="IV329_000509"
     CDS             complement(19683..22325)
                     /gene="alaS"
                     /locus_tag="IV329_000509"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8939716.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22713..23144
                     /locus_tag="IV329_000510"
     CDS             22713..23144
                     /locus_tag="IV329_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939717.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23189..24538)
                     /locus_tag="IV329_000511"
     CDS             complement(23189..24538)
                     /locus_tag="IV329_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8939718.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24608..25771)
                     /locus_tag="IV329_000512"
     CDS             complement(24608..25771)
                     /locus_tag="IV329_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939719.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25900..26223
                     /locus_tag="IV329_000513"
     CDS             25900..26223
                     /locus_tag="IV329_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939720.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26406..27755)
                     /locus_tag="IV329_000514"
     CDS             complement(26406..27755)
                     /locus_tag="IV329_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8939721.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27824..28777)
                     /locus_tag="IV329_000515"
     CDS             complement(27824..28777)
                     /locus_tag="IV329_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8939722.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28879..30069)
                     /locus_tag="IV329_000516"
     CDS             complement(28879..30069)
                     /locus_tag="IV329_000516"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8939723.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8452 a         6636 c         5315 g         9757 t
ORIGIN      
        1 attctcttct ttctgctaac cagtcttaga aaaatgaatt ttaaaaaaat caaaaactga
       61 tcacgataga agtttctttt catacctgat gaaaagatgc ttctcgcaat cagtttttgg
      121 atttttgcta ttccaattga tgattctgga gacacaaatc ggatttctct atcttcatca
      181 agcgcgcttc aatctgccaa ttttcaggat cttttttcca ttcttttagc agagttaatt
      241 cttgctcatc gatataacga tcttctaaag ctgcttcaat caatacagaa taattggtca
      301 acgtcataag agggatttcg gctttttcga aatttgcttt tccttttggc aactcatacg
      361 taaagatagc cgcaacacca aggatatccg caccttctct ttttgctgct tcagctgctt
      421 ccaagacact tccgcctgta gaaatcaaat cctcgatcac aaccatcttc tgaccttcaa
      481 atatacgtcc ttcgatttgg ttacctttcc catgatcttt cgccttactt cgaatataga
      541 ccattggcaa atccagtatt tccgctaccc atgcggcatg agggattcct gctgttgcag
      601 ttccagcaat cacttgtact tcagggtaag cttctttgat tttgcttgct aatccttttg
      661 cgatctcttt tcggactttt ggatagctca tcgtgattcg gttatcacag taaattgggc
      721 ttttgattcc acttgcccat gtaaaaggat tacttggatt taaaaatact gcttcaattt
      781 ctaataagtc ttttgcgata ctttgttcaa cattcatttt aggttagctc cattctgttt
      841 gaatcgtttg atatgcttta taaggattag ttgactgagt gattggtcga cctataacga
      901 taaaagttga gccgatcttt tttgcctgag aaggtgtgac tactcttgtc tgatctcctg
      961 cgactgttcc ttctggtctt atacccggag ttaagcaaac aaattcgtct cctgccattt
     1021 ctttgatcgc ttctacttcc caagcagaag aaaccacacc atctaatcct gcttttttgg
     1081 cacagctagc ataatggatc acactttttt ctaaagaaac ttcgattagt tgatcggcat
     1141 gcatttcttc ttcacttgta gaagtaagct gagtgatagc tagaagtagc ggacgttttc
     1201 caccttctgg agtcccctct tctaatcctc gcattgccgc ctccatcata cgaataccgc
     1261 cagcggcatg aacacaggtg agatcaacac caagctttgc caatccccgc attgcttttt
     1321 cgaccgtatt cggaatatcg tgaagtttca gatctaaaaa tacattatga ccagcctctt
     1381 ttagataacg tacgatttca ggtccttctt ggtagaacaa ttccattccg attttcacaa
     1441 acaatttttc ttctttcggg aaaaatcgaa gaaagtcttc tacctcttgc ctagttgaaa
     1501 aatctaatgc aataattggt cgttggtcta gttgattcat cgttcacgtt cctctctcac
     1561 ttctttgatt aatctttcca gcgattcgat gcctagttcg ctcattcgca cagggagctc
     1621 tttaattagt ttaggacaga tatacggatc ggtgaaattc gcggtcccta tagctacagc
     1681 acttgctcca gccataaaca tctcaagaac atcatccact gtctgcacgc cgcccatccc
     1741 aatgattgga aggctggata tacttgctac ttggtgaatc aaacgaatag cgacaggctt
     1801 gattgctggt ccggagagtc cacctgtttg attggctaaa atcggttttc ttgtcttaag
     1861 gtcaatccgc ataccaagaa gagtattgat catcgtaaac ccatctgccc ctccagcttc
     1921 gattgcctga gcgattggaa cgatgtctgt tacatttggg gacaatttga catagacagg
     1981 aacagaagaa actttcttta ctgcttgtgt taattgaaaa gcaacatcag aatctgttcc
     2041 aaaagcaatt ccgccatgtt tgacatttgg gcaagaaatg ttcagctcga ttgctttcac
     2101 atttggtgct tcgccaattt tgctacaaac ttctacatag tcttcttcac aagcaccagc
     2161 aacatttgcg atgatgggta actcattgta ctcctctaat tctggaagaa gtttatgcat
     2221 aacaacgtct agaccaggat tttgcaaacc aattgcattc aacatgccag atggtgtttc
     2281 cgccactctt ggcgtgggat tgccaaagcg ggcatttgga gtcgttgcct tgaccatgat
     2341 cgatccaagc tgattcaaat catagtattt tccgtattct ttgccaaaac cgaaacagcc
     2401 gcttgccggc atgataggat tttttaattc taggccagga agtttgaccg ctaaaggatt
     2461 ctcattcata taattacctc tcctatttta aaaatcggtc cttcgtcaca aactttcaca
     2521 ctttttgttc ctgtttcatc ttccggcgta tgacaaacgc aagcatagca agcccctatc
     2581 ccacatgcca tgcgggattc taatgataac tggacatttt gatgcgaaga aaacagctgt
     2641 tccactgtct tcaataaccc gtttgaacca catgcaaaaa cagctgtagg ttccgtcttc
     2701 gttcctaaca ataaattacc gacatttccg tgcataccgt atgttccatc gtcagtagcg
     2761 attcttgttt ctcctagacg attaaattct ttttcataat aaacgacttc atgtgtggca
     2821 aaacctaaaa aatgcgttgg cttaattcct ttttcgacta actgttttga taactcatat
     2881 agaggaggga caccgattcc tccaccgatt ataaatactt cgtctcctgt atgaagattg
     2941 gacaaatcaa aaccattgcc taaaggaccc atgacatcta aatggtctcc ggcagaaaga
     3001 gtagagaaga ctctagtccc ctcaccttcc acccgataaa tgatacggca agtatttgtt
     3061 tcgtggtcga tatgattcaa gctaatcggt cggcgtaata gcaagtctgc acgcggcacg
     3121 agaatatgca agaactgtcc tggttgctgc atttctttta ctagatcacc tttcaatgtc
     3181 atttcaaaaa tctttggtgc cagctgtttt tgctgaacga tttccatcat ttcttgtttc
     3241 atctgtcccc tcctgcaacg gtttttcgtc tttttgtctt aacatgtgct cgccgttaaa
     3301 tagggttagt tgaaaaagca cgggattcca tcactcgaac gatcgcatct gctgtgtcta
     3361 aagaagtaaa caatggaata ccatgttcaa ctgcttctct tcggatgatg aaaccgtctt
     3421 tagaagcatt tccccgatct ttatcaatcg tattgacaac cacttgtgtt ttaccatctc
     3481 ttattaagtc gactacattt ttttctgctg cttcctcaga aattttcgct actgtttcaa
     3541 cgaccagacc atttttttcg aaataagcag ctgtatgttt cgttcctatc agactatagc
     3601 caatttctgt aaaacgttta gctaattcta gtgcttcttc ttttgtttcg tccgcaatcg
     3661 taaataatac ggctccatat tcaggcaagt gtaatccaga agcttcgaat gccttgtaca
     3721 aagctttgtc taattgtctg tcagatccca ttacttctcc agtcgatttc atttcaggcc
     3781 ctaagtaagt atcaactttc tgcaatttcg taaaagaaaa gacaggtgct ttaacatgaa
     3841 cttcttttgt ttctggataa agtccgtttt cataaccaag tgtctttagt ttctctccca
     3901 aaatagcttt cgtcgcaatt tgtgccattg gaattcctgt caccttactt aagaatggaa
     3961 cagtccgact tgctcgagga ttgacttcga tcacataaac ctcattttca tggatgacaa
     4021 actggatatt catcatcccg acacaattca aacctaatgc taagcggatc gtgtaatctt
     4081 cgatcgtttt cttgatcgat tcagataatg tttgaggtgg atacacagcc attgaatccc
     4141 ctgaatggac accagcacgc tcaatatgct ccatgatccc tgggatgagt actgtttcac
     4201 cgtcacaaat cgcatccact tcgcattcac caccaagcaa gtaacggtcc actagtactg
     4261 gatgttctgg agatgctttc acagcatgtt ccatgtaatc ttctaaatct cgctggtttt
     4321 caacgatttc catcgcacgt ccgcctaata catagcttgg acgaacaaga actggataac
     4381 caattctcga agcgataacc acggcttctt ctttacttgt agcagtatcc cctaaaggtt
     4441 gaggaatatc caactctttc agcgcttgct cgaataaatc acgattttcc gcacgatcca
     4501 agtcttcgat cgttgtccct aagattttta caccagcagc agtcaaaggt tcggctaagt
     4561 tgattgctgt ctgaccacca aattgaacga ttactcctat cggctgttct agctcgatca
     4621 cattcatcac atcttcgaag gtcaatggct caaaatataa tttatccgaa atagaaaagt
     4681 cagtcgaaac tgtttcagga ttgctgttca tgatgattgc ttcgtatcct gcctgctgga
     4741 ttgctttgac agaatgcaca gtcgcatagt caaattcaac cccttgtccg atacggatag
     4801 gaccagatcc cagtactaga attgagggct tttctgatcg tgaactttca ttttcatatt
     4861 cataggaact ataaaaataa ggtgtttttg attcgaattc cgctgcacaa gtgtcaacca
     4921 tcttatatac aggaatcaaa tcattttctt ttctcatctg gcgaacggct tcttcgttta
     4981 tttcccacag ctccgccact tttttgtctg taaaaccgtt ttgtttcgct gttttcaata
     5041 tcttgtcatc caacggctgc tgtttcaact gctgttcgat ttctacgata tgcaacagtt
     5101 tatctaaaaa gaataaatcg atttttgtca aatccgctaa ttcttctatg gtgtaccctc
     5161 ttctaatagc ttcagctaca taaaacaggc gatcatcttg tgctcgaacc actttttgga
     5221 ttaggtcatc gtccgtgatg tctgccaact caggaagttc gatatggtgt gtaccgattt
     5281 ctaaagaacg cacagctttc aataaagact cttcgatatt ccgaccgata gccatcactt
     5341 caccagttgc tttcatctgc gtgcctaaaa gccgttctcc gttttcaaac ttatcaaacg
     5401 gccagcgagg aattttagct acaacataat ctaatgcagg ttcaaattct gcaaaagtcg
     5461 ttcctgtaac aggatttttc atttcgtcta atgttaatcc aagcgctatt ttagcagcta
     5521 gcttagcgat cggatagcca gtcgccttac ttgctaacgc agatgaacga gaaacacgcg
     5581 gattgacttc aatcacataa taattaaaac tatgtggatc aagagcgagt tggacattac
     5641 agcctccctc gatcttaagt gctcgaataa tcgaaagaga agcatcacga agcatttgat
     5701 attcatggtc agacaatgtt tgactagggg caaatacgat agaatcacct gtatggatac
     5761 ccaccgggtc aaaattttcc atattgcaga caacgattgc attatcagcc gaatctctca
     5821 tcacttcata ttcaatttct ttaaaacctg caatacttcg ttcaatcaaa cattgggtag
     5881 cgggggaaag acttaatcca ttttctgata cgatacgtaa ttcttcttca ttttcacaca
     5941 tacctccacc agttccgcct aaagtaaagg ctggacgaac gatgacagga tagccgattt
     6001 tatttgcaaa atctacagct tcttctactg tattgacgat ttcagactcg gggattggct
     6061 gattcaattc tttcatcaac tgtttgaata gatcacgatc ttctgcttga tcgatcgcag
     6121 aaagctttgt tcctagaagt tctatgttca attcttctaa aatacctgat gctgccagct
     6181 ccattgccat attgaggcct gtctgtccgc ctaaagttgg aaggagagca tctggacgct
     6241 cttttcgaag aatccttgat acaaattcaa gtgtgatcgg ttcgatatat acttgatctg
     6301 ccacttcttt atccgtcatg atcgtcgcag gattagaatt tactaaaacc acttcatatc
     6361 cttcttcttt taaggcaagg caggcttgtg ttcctgcgta gtcaaattct gcggcttgac
     6421 caatgatgat cggaccagat ccaatcacca taattttctt gatatctgta cgtttcggca
     6481 tctgtttact gctcctttcc tgcgtccatc atttccataa attcatcaaa taaatgtagt
     6541 gcatcatgcg gaccaggggc agcatctgga tgaaactgca cactgaaagc gggataatct
     6601 ctgtgtttga ctccttctac tgttccgtca tttacttcta catgagttac gatgagtttt
     6661 tctggatcaa tcgtggcttc atcgactgca tagccatgat tttgagaagt aaaatcaatt
     6721 cttccggtcg ctacttctcg taccgggtga tttaatcctc gatgtccgaa tttcatttta
     6781 taggtatctg caccgttggc taaagcaaat aactggtgac cgagacaaat accaaaaatc
     6841 ggtacttttc cttggatttt tttgatcatt tcgattgctt ctggcacatc ttttggatca
     6901 ccaggtccat tcgtcagcat gactccgtct ggtgatagcg ctaaaatcgt ctcggcatct
     6961 gtgttatagg gaagtactgt gaggttacag tttcttttac tcaattctcg taaaatacta
     7021 tgtttcaagc cgaagtcaat gacaatgact ttccgacctg ttcctggact tggataaggt
     7081 ttgttagttg agacttgctg cacttgattg gttgctagaa cggttgcttt cagctgatca
     7141 aatgcatgtt cgaaggaatc tccagcatca actatactag ctttcatcgt tccgacttca
     7201 cggattttgc gagtaagggc gcgcgtatcg atacctgata ttccaggaat tcctttttgt
     7261 ttcaaaaatg aatctagcgt catttggttt cgccagtttg aagctacacg tgcatgttct
     7321 tttactacta ctcctttgca tgttggagaa atcgattcat aatcatctct atttacccca
     7381 taattaccca ccataggata ggtaaatgta atgatttgac cgttaaaact ttgatcagtg
     7441 atagtttctt gataaccagt catacttgtt gtaaaaacga tttctccgaa gacactagca
     7501 tctgcaccaa aagcctttcc ttcgaaaact gttccatctt ctaatataag caaacgttcc
     7561 atacttttat tctcccttct gccacacaag ttttccgttg acaaaagtca tttgtgtttc
     7621 gccttttact ttccagccga taaaaggtgt attctcccct tttgataaaa agtcttcttt
     7681 atcgatcgtg caagtttgtg taatatcaaa aactgctaca tcagcggggg caccaaccgt
     7741 tagcgttcct gcattcaaac caaaaatctc agccggttta actgccaacc aattgatcac
     7801 ttgttccaac gtgaatcgac cagtttctac aaaatgagta tagatcagtt gaaacgctgt
     7861 ttcgcttcct acgataccaa atggtgattt catgaaactt tggctttttt cttctaatcc
     7921 atggggggca tggtctgtag cgatgcaatc gatcgtaccg tcaagcaagc cttcgatcaa
     7981 agcttctctg tcctcccgtc ctcttagcgg cgggttcatt ttccaaaagc cgaaatcttc
     8041 gggaatatct tcgtcgatca aaataagatg atggggtgaa acttctgctg ttacgtggat
     8101 tcctgctttt tttgcgtctc tgattacacg gacactttct ttagtagaaa catggcaaac
     8161 atggtaatga acaccagttg cttcagctag caacagatct ctagcaattt gagaagactc
     8221 ggttacgctt aagatcccgg gaagaccaag ttcttctgcc ttcttgccag cgtgcataac
     8281 gccgccaaat aataaagatt catcttctgt atgggctact agggctttat tgttttttgc
     8341 agcttctttc atagccagat acatcgtccc agctgtttgg acacctacac cgtcatttgt
     8401 gaaagcaaaa gcaccttctt caatcagtgc ttcttgatca actaatttct cacttcgcag
     8461 attttctgtg attggcgcat actgcaatac cttcacaact gcatcttttc taatgatgtc
     8521 atatacttgt ctcagctttt ctgccgtatc aggtacagga tttaaattcg gcatcgcgca
     8581 tacagtagtg aaaccgccac gagcagctga gcgagtccca gcttcgatcg tctccttgta
     8641 tgtaaatcct ggctctctca aatgaacatg gacgtccact aaaccaggtg tgatcaattg
     8701 tccctttgca tcaaacactt catcaaattc ggcttctgag aatcctgtac cgattgcttt
     8761 gattcgccca tcttctatcc aaatttctgc tggtgtcgtc atattttttc gtgtattgat
     8821 ctgaccgttt ttaatgagtg ttttcatttt ttcatcctcc tataattacg cttttccatt
     8881 tagcactgct tctaagattg ccattctgac gaatactcca ttgctcattt gctgaacgat
     8941 ccgcgactgt aatccttcca ctaatgaatc agctaattct acatcacggt taactggtgc
     9001 tgggtgcata atgattgcat gtttttgcaa cttcttcgca cgctcaactg ttaacccatg
     9061 ttcctgatgg tagccttctt ttgagaaact ttcttttcca tcatggcgtt cgtgttgcac
     9121 ccgaagcatc atcatgacat caacttgatc tagtagttca tccaatggca tataatgtcc
     9181 gtacacttcg aattcatcgt cgtaccattc tctaggacct gagaagaata cttgtgcgcc
     9241 tagacgtttt aacatctgca tattggattt agctacgcga gagtgagtga tatcacctac
     9301 gatggcaact ttcagacctt caaaatgacc aaattcttca taaatggtca tcaaatcaag
     9361 caagcattgg gttggatgct gtccgcttcc gtctcctcca ttgataatcg aacactggat
     9421 tgttttactt tgtatcagtt cgtcataata gttctcatca ccatgacgga taaccgctac
     9481 gtctacgcct aatgctgaca tcgtcagtac tgtatcgtat agtgtttccc ctttttggac
     9541 agagcttgtg cttgcttcaa attcaatcac ttctagccct agttttttct ccgctacatc
     9601 gaagctttta tgtgtgcgag tactattttc gaaaaagagg tttgtagcaa agtactggcg
     9661 cttttgcgga gtccaattag caccttgctt gaattcctgc cctctgcgga tcaagcccat
     9721 aacttcttga tcagtaagtg cctcaacggt caataaatgc tttaagctga tacgttctga
     9781 tttgatgatc ataatgtttc ctccttagtt ttcttcgctt cgtgaagttt ctggtaagat
     9841 aagatttaaa atgatgccaa gtacagttgc taatgccatt gccgacaatg taaatgtgcc
     9901 tacttcaaag acgagtccgc caattcctac tactaagata actgaagcaa tcaacagatt
     9961 tttcttttta tcaaaatcga ttttgttctc gattaaaatc ttcattccac ttgcggcaat
    10021 tactccaaat aagataaaac tgattcctga gatgactggt ccagggatac ttaaaatcaa
    10081 agcgcttaat tttcctacga atcctaaaat gactgcaaat actgcagctc caccgatcac
    10141 aaacacgcta tggacacgtg tgatagccag aacaccaatg ttttcaccgt aacttgttac
    10201 aggaggacct ccgacaaagg ctgccacgat ttgtgctaat ccatcacctg tcaacgtctt
    10261 atgaagaccc ggttcttcaa agaaattacg tttagttagt ttattcaata ccataagatg
    10321 gccgatatgc tctgtcatcg tcacaaaagc gatgggtgcc attgttgtga ttgctccaag
    10381 atatagcttt ggttcatatt gtacaaaggg aatctcaaag ttaggaacag caaaccaggc
    10441 agcgtctacg attggcttag tatccacgat tccaaacagc aaggctacga tgtatccgct
    10501 aataatacct agtaaaatcg gaatcaatcc gagaaaacct cttagccaca tgttgaagaa
    10561 aattgttaat cctaatgtga tcagcgctac agcaatatac ttgaaatcat acgtgccctg
    10621 attatacata gcatttgttg ctgcatttgc cgcaagtccc aacccgataa ccatgatgac
    10681 tggaccgacg acgattggag gcaagatttt atccagccat gctgaaccga tctttttgat
    10741 gatcagcgca acaatcaaat agactaatcc agttgtgact gctccttgtg caatcgctgg
    10801 ataaccatct gttttcatca aaagctgcat cgctgcaatg aaagcgaagc tgcttcctaa
    10861 gtacgcaggt atctttcctt tagtagtaaa taagtacacc aatgtaccta gtcctgaact
    10921 gactagcgca atacctggat cgatacctac taagatgggg actaacacgg ttgctccgaa
    10981 catcgtgaac aagtgttgta aacttaatcc tacccaatgt cctggggttg ggcgatcgtg
    11041 gatatctaac accacgtcat cattatgaaa tccttttttc tccgacatgt ttctctctcc
    11101 tctaatcttc ctctttcaaa atcatgatgc gatcttgtcc atcaagctct tgcatctcta
    11161 ctaaaatctc ttccgcccga gatgttggaa tgttcttccc aacataatct gcacggattg
    11221 gcaattctcg atgcccacga tcgaccagga ctgctaatga aatttttctt ggtctgccaa
    11281 aatccattac tgcgtccatt gctgctcgaa tcgttcgtcc tgtatacaaa acatcatcaa
    11341 tcaaaataac ttcttttcct tctaaggaaa ctgggatgtc agaagaatga agttccggtt
    11401 cttctggatt ttcttttttg tcatctcggt ataaagtgat atccagttct cctacaggaa
    11461 tatcgatatc ttctagctgc ttcaggcgtt cggcaattct tgatgcaatg tagattcctc
    11521 gtgttttgat acctaccaat acgatatctt ggatactatg gtttctctcg ataatctcat
    11581 aagtaattcg agtcaatgct cgcttcatgg ttacctgatc tactacttct tttgcttgca
    11641 ttttcattcc ccctactttt ttgcataaaa aaactcctgc cctaagagga caggaggaat
    11701 gtttagactg agcgtatact gatacactga tagctatact atgtcaatga gcaatctact
    11761 tatggcgcac catacgtatc cttgctccca cccttcttag cctcacggga ctaaacttaa
    11821 aggaattcta ttaagtttta tgctttcgtt gttaattact ataccgttat ctaaattaaa
    11881 atgcaatgtt ttttcttaat ttttctaaag ttttttcaaa aacttctgga agaggtgctt
    11941 caaagatcat cttttcgttt gtagtcgggt gcgtaaaacc taacatttgc gcatggagaa
    12001 actgaccatt gcctttcaaa gttttcttcg gtccgtatac tggatctcca gctaacggat
    12061 aaccgatata cttcatatgc acacggatct ggtgtgttct tccggtttct agttttaatt
    12121 caaccagagt aaatccttcg aaacgttcca atacagtaaa atgtgtgaca gccgacttcc
    12181 catcttctct tatcgcctgc attttccggt tttccttcga acggccgatt ggcgcattga
    12241 tggttccttt ttcgtgagga atctctccat ggactaaagc aacatatttg cgcaaagagg
    12301 tcttatcttt taattgctga gctaatgatt cgtgtgcctg gtcatttttg gcaaccatta
    12361 gcagtccgga agtatctttg tcgattcggt gaacaatacc tggacgaata acgccgttga
    12421 tcgtagaaag atccttgata tggtacatca gcgcattgac cattgtgcca tgcgtatgac
    12481 ctgctgaagg atgaacgacc attccttgag gtttgtttat caccgctaca tcttcatctt
    12541 cgtaaacgat cgtcaacggg atattttctg ggaccacatc taatgtttct ggttcgggga
    12601 tcaagacagt aatctcgtct ccttgtttta ctttgtaatt tgctttgact ggctgtccat
    12661 tgacagaaac cgctccattt ttcagccaca gctgaatttg cgaacgactg taatcggtga
    12721 ataaattagt caatacttta tcgatcctac cagattcatg gtgaatcgtc gttttaagtt
    12781 ctgtcatttt tttctttgat ccttttcatc taaaataata taaattaaca tacaaatcac
    12841 tccacataca agtgtcacat ctgctacgtt aaaaatcgga aagctcataa aatctgtctg
    12901 gaacatatct actacatagc caagacgaat acggtcgata aaattgccaa tcgcaccagc
    12961 tagaatcaaa ctcaatccaa tcatgaacca gcgattacct ttttcacgat ttttaatcat
    13021 caatgtgaga atcacaacga ctgcaatgag tgtcactata gtaaagaacc acatcttccc
    13081 ttctagcata ctccatgcgg ctccagtgtt tcgaatatga ttcagtgaca aaatacctgg
    13141 tataagattt ttcgtctctc caagcgctag attatcagtg atccaccatt tgaccaactg
    13201 atccactcct actagcaaag cgctgaatat ccaataaata attaacaaaa aatcaatcct
    13261 tttctatttg tttataaata tcacgaggtc gtacgatccc gagcagatta ccatcgggat
    13321 tcccatcttt agtgataagt acggcttcca aagtcccttg ctcacgaaac atcgcttcaa
    13381 cctcgtaaat atgtgtttta gctggaacaa aatgataatt tgtgtacttt ccgttttgga
    13441 tgatcaaatc caacgctcgt ttatttgtta aatcaaccgg acctttttgg ctttcttttg
    13501 ctagccaaaa cccgatatta cgtaaagtaa tcaatgctac aaattttcct ttattgtata
    13561 acggaaattg tgaatacctt ttttctgcta ctatctttaa cagttcaagt aacggaagat
    13621 cttgttcaaa acctgttaca cgttttgcaa atcgaggcag cacggtttca ggctgaatca
    13681 gttcttgttc gatcagctgg attcgcttga ctgtccaatc atttggctct gcaatgacaa
    13741 aatcgactgc gatccgatca tggacgatcg cattccttaa ctgagcaagc tgtaaaagat
    13801 catcttcgta ttttctgatc attaattgtt ttctttgagc taggcgtctg acaagttcgg
    13861 taaatcccat atttcgtgca tttcccatct cgtctctcat ccatttttcg attcgattga
    13921 aactgattaa aaaaagatca gagtttgcca taattttcct ccttgatcaa atatgcccgg
    13981 ttttttatat agaaaaagaa cgcaatcaac acaataactg caaaaaaact gaaaaataaa
    14041 ttttgtagga aaacggcccc caacccagtt gctagaaata aaaacggata gatttgaaga
    14101 atcaagtaat tcattgtgat cacataagca ggctttgctt ctttcactgt ataaacgggg
    14161 ctttttagcc aataaaaggt gacctgcatc gtaaagggca cttccagatc aagcgttatc
    14221 ttttgttcat gactgagaac actgcgtttt tcattgttga ttcttagctg aatcgagcta
    14281 cccataccgt aaaaaccggt ttttcttata aatgtcgttt tcatcatacc ctcctattat
    14341 gctttagtat agctcaaaat acaataaaga aaaagaatga tcctagtgta aacaaaatta
    14401 attccctatc gtctaaaaaa agaagctgta gtcaaaatac agctccttcg atggtagatt
    14461 ataatcaagc ttaaaacaat cccaacactt ctccgtcatt tgtcacgtcc atattcaatg
    14521 cagcaggttt tttaggcaaa ccaggcatcg tcataacatc tccagtcaag gctactaaaa
    14581 atcctgcccc taattttggg atgatttcgc ggatcgtgat ggtgaaatct tccggtgcac
    14641 ctagcagtga aggatcatca gaaaatgaat actgtgtttt tgccatacaa atcggcagtt
    14701 tgtcccatcc attcttcgta aactcttcta tttgtttttg cgcttttttg ctgaaaacaa
    14761 caccatttcc tccgtaaatt ttcttgacga ttgtttctgt cttttcttgt atcgtttctt
    14821 ctaggcggta taatggcttg taattagctt cttttctatc aatcaatcgt actacagctt
    14881 ttgctaaatc aacgccgcct tctgctccat ctgcccatac actcgctctc ttggcaagaa
    14941 tcccctgatt ttcacacaag tgctccaaca gtgtgagttc attgtctgta tctgttacga
    15001 attcattgat tgcgacgatc acaggtagct gatattgttc catattacgt atatggcgct
    15061 ttaaatttgc aaaacctact ttcaaagcat caagattctc atttttaagt tcatcttttt
    15121 tcattccgcc atgcattttc aatgcacgga cagtggcgac aatcaccaca gcatccggcg
    15181 cttttttcaa gttaggtact ttgatatcta aaaatttctc tccgccaaga tctgcaccaa
    15241 atcctgcctc tgtgacaacg taatctgcta atctcaatgc tgtcttggta gctaaaatac
    15301 tattgcagcc atgagcgata ttggcaaacg gtcccccgtg gacaaaagct ggtgttccgt
    15361 aaattgtttg tacaagattc ggcttgattg catctttcag cagtaatgcc agagcgcctt
    15421 ctgctttcaa gtctccagca gtcacaggct gacggtcaaa tgtatagccg acaacgatcc
    15481 ttgcaagacg agcctttaaa tcttcaaggt ctgccgcaag acacaagata gccatgattt
    15541 cgctggcaac tgtgatatca aacccatctt ctcttggaac accttggatt ggcccaccca
    15601 agccaacaat tactttacgc agttcacgat cattgagatc gactacccgt ttccaaatca
    15661 ctcggcgggc atcgatgttc aattcatttc cttgatgaat atgattatct aaaagagcag
    15721 ataatgcatt attagctgtt gtgatagcat gcatgtcacc agtaaaatgc aagttgatat
    15781 cttccatagg caatacctgt gcatatcctc cgcctgcagc accacctttg attcccatca
    15841 caggaccaag tgagggttct cgcaatgcaa taacagattt tttattgatt ttattgagcg
    15901 cgtctcctaa ccctacagtg atcgttgact ttccttcgcc agctggtgtt ggattgatgg
    15961 aagtaactaa gatcaaatgc ccctcagcgt tatcctttga tcgattgatt ccagaaaaat
    16021 tgattttcgc cttatatttc ccatacagtt ccaaatcgtc ttccttaaga tctaatttac
    16081 tagccacttc aacaatcggt tctagctctg tttcttgtga aatttgcaga tcattcttca
    16141 tttgattacc ccttttcacg aactttattc aacaaaatat cttctttgtt aagatatgcc
    16201 aattatacct ttttttattc agaaaaaaaa cataaaatta caggttttta atttatttga
    16261 cgtacttata aatactcttt ataatgaagg ctatgatcag actttagaaa ggtcgtgagt
    16321 gctttgacca caggaattgt aaaatggttt gacaacaaaa aaggatacgg ctttatcagt
    16381 tatgatgaca cagaagaaat ttttgtacat tttactgcga ttgaagaaga gggattcaaa
    16441 actttggaag aaaatcaagt ggtcgaattt gagatcatcg aagggaatcg aggaacacaa
    16501 gctgcccatg tcaagaaagg caacgttcag actaccgata ctagtatgca tgaatagata
    16561 aacaaataga gtgagacgct aaacgggatg aaccgtacgt ctcactcttt tcttttcctg
    16621 taaaaattcg tttttttagg ttctgatttt taaaattagc tttttctttt tttcgtataa
    16681 ggataaggaa ttgattctga tttgggtccg tttttttcaa aatgatcaaa aaggactact
    16741 tgatcttttt ttcgcatcat gaaaacagat ggggctttct catcttgcaa catcatttgc
    16801 accccagtat tagttggcgt caaagatcga atctgtccga cagatatttc tcggtctttc
    16861 caaaaagcag cataacgtat aatcacagag tttgatttta aaataaaaga acgccgcaaa
    16921 cctagaaata gaaaaacaag aaaaatacct aaaacaaaat tgcttttcca ataaggacct
    16981 gtgttttcta aagaaagaat cagactaaaa aacagaatga tcaaggtcaa tgaccaatag
    17041 atgatcgtat gtgccagttc aggctgccat cgaatatttt ttttcattct ctaacctctc
    17101 accgattcag taatgatata atcttaccat aacattagtt tgaacaaaaa ggagaatctc
    17161 tttttatgtt aaaagtatac attgatgcct ctacaaaagg caaccctggt cctagtggag
    17221 gtggaatctt gattgtctat caaggtaaac aagaacagct atctatcccg ctttctatag
    17281 ggacaaacca tcaagcagag ttcgaagtct ttttaaaaac tttagaattt ctaaagaaaa
    17341 acgacttaca gaatgaaacg attttctgtt tttcagacag caaaacacta gtttctacgg
    17401 tcgacaaaaa cgcaacgaag aacgagctct tccttcccta ccttcaaaga atccaagaac
    17461 ttctatccga attttcttta ttgatcctgc aatggttgcc cgaaaacaaa aataaaggag
    17521 cagataatct tgcacgtcag gctctccata aacaattaaa agaaaaaaag actctttaaa
    17581 ttaaaaactt taaggagtct ttttatagtt tctcatttaa tataaaagat aattgtgatc
    17641 aaacgtaatt tccaattggt aatctccgat tttttcttct atttgactac gtaagattct
    17701 ttctagttct gtatcaacaa actggaattt ctcagggacc actacatcaa attgcagcgt
    17761 ttttccttga tgaattactc taaaatcatg aatattaagc cctgtacctg tttcaagaag
    17821 aatttgcttt aactgccgca agatattata gtatttttcg ttatcaacag gcattggatc
    17881 caagtgacag acaagttcca catccaaaga ctttttaaag tctttttcta tcagatcaat
    17941 aatcttatga gccacattca aattcattga ttcatctact tcgatgtgga ctgatgcaaa
    18001 gcgtttattg ggtccatagt tatgcaccaa taaatcatga tagccaagta tatcatcgca
    18061 agcatttaaa tgctcttcca tttcttcaat ctctgctggc gttgggcgac ttcctaatag
    18121 ttcgtataca aaatttcgaa gcatcgtcaa tccactataa aggatatata atgctaaagc
    18181 aaaacctata tatccatcaa tacgccatcc agttacccat tcgattgctg ctgaaagcaa
    18241 tacagccagt gtagtaaaga catcattata actatctgtt gctgtagctc tcaatgtttc
    18301 agaatcaatg gattgcgcaa taacctgata cattttgcct tgtgcaaatt tcaaaaagat
    18361 ggaaatgatt aggacaaaaa agacgatggg tgttaaagag acattttctg gatggatgat
    18421 tttttcagca gaagactcta aaaattgaaa tcctacataa gtaatgatga tcgaaacaaa
    18481 taacccactg atatattcga agcgttcgtg tccataagga tgttcctgat ctgctggctt
    18541 tgcagcaatt ttaaatccga tcaaagtcaa tatggatgaa gcagtatctg atagactgtt
    18601 cactgcatca gccataatgg agacacttcc tgaaaaaagc ccgatcatta gcttagagac
    18661 aaacaagatc agattcgtaa ccaatcctaa gatacccgct agtactcctt tttttgtccg
    18721 ctgttgcggt atttgtttct ttttttcttc ttgtttctta gccaagtttt ttaccccttc
    18781 tagtttgctt gttgctcagg taaaaagatg gagaggacga ttgttttcct ctcctctctt
    18841 cctttttcta tctatcgtgc gtcctattac tattaaaaga tggtcattat tctggtttca
    18901 acggtgttgc tgcaaataac gcttttgcta gtaatggtgt tatgattccc gcacctattg
    18961 cttctggtat cccattgatt gctgcaaccc ctgctaaggt tttaaataaa agagcagagt
    19021 ctacaccata ggcatctgcc gttgcacctg tataaaataa tcccatgaat cctaacacta
    19081 agactgtatt gaccaatgaa cccagtaccc ctgcaaaggc tgagctgatc gttattgatt
    19141 tatttttttg ataaatccag tggaaaacca agcctgctac taagccaaca agtactctgg
    19201 gaactaccga gataatcgga ttcgtgaaca ctaatgtatc aaatggcgtt gttggcatgg
    19261 cataggcacg aacaattgta gcgattcccc aaaacaagcc aatcaccatg ccatcttttg
    19321 ggcctaatac tactgcagca ataattactg tgatatggat aattgttaaa ctaatgaatc
    19381 ctaatggaat aaaccccagc caaggaacca tcgcttgtac gatgatgatt gctaacaaga
    19441 tcgcacgtaa gactagtcga aaggtcttat ttctactatt cattttttca ccccttttta
    19501 tttatactaa tcgtaaaaat ttctgtcact ttcgtcaact aaagaccatc cgaatccgac
    19561 catttgatta gatgcgccat ccaattgaaa aaagtatgag ggcaagactt gtttgtcttc
    19621 gcccccataa tcatgtttcg ttcaatgttt gaacttttgt ttttctgtca aacgtaaacg
    19681 ccctattgtt gcgcaaccca ttctttcgca gctttaagtg catctggaat accagcagga
    19741 tttttcccgc cagcttgtgc catgtctgga cgtccaccac cgccaccgcc tactttcggt
    19801 gcgattgctt taatcaagtc gccagctttt agtccagctt catttgattc tttcgtcatt
    19861 gccgctagca gactgacttt tccttcttgt tcgtttgcta aaacaagaat atctgaaata
    19921 tttttttgtt tccattggtc agctaattga cgcagctgat tcatatcttt cacttttact
    19981 tgtgctgcaa tgattgtttt tccattgatc gtttctggat ctttgaatac atcaccagct
    20041 tgttgattcg ctaacttgct agctaattgt tcattttcct tttgtagctc tcttacttgc
    20101 tgttgcaatt gttccgcttt tgaaacaact tctttcaatt gaggtgattt aacaattgtt
    20161 gctacttgtt ttagttgttt ttcttcttct tcaagtagtt cgtacgcttc tttacttgtt
    20221 acagcctcga ttcgtcgcac gcctgcacca attcctgatt cagaaacgat tttgaagatt
    20281 ccgatgtttt ctgtatttgc tacatgtgtg cctccgcaca gttcgattga ccagccgcca
    20341 atatttacta ctcgaacgtc atgtccgtat ttttcaccaa acaaagccat tgcacccatg
    20401 tttttggctg tatcgatatc tgtttcaatt gtttcaactg ggatagcttc ccagattttt
    20461 tcgttgacga ttctttccat ttcagctaat tcatctgaag tgatttgacc aaagtgggta
    20521 aagtcgaatc gtaaatgtcc aggagcgact aaagaaccag cttgatttgc atgctcgcca
    20581 agtacttctt tcaatgcctt gtgcagtaga tgggttgctg tatggttttt gatgatccga
    20641 ttgcgcatcg attgatcgat caatagatga taggtttgtc cttcactcat tggtgctttt
    20701 acttctacgg tgtgcataaa ttgaccatta ggcgcttttt gaacatctgt aacaactgct
    20761 acgatttcgc ctacttcatt catgatccat ccttgatccg ctacttgccc acccatttct
    20821 gcatagaaag gtgtttgatc aaagatcatt tgagcttcac cttctgcgac ttcattcaaa
    20881 agagactcac ctttaacgat gaccaataat tgactatctg attcttgttc tgtgtatcca
    20941 atgaatttac tttctacttt gatatctgtc aataaagcag attgtacccc cattgatttt
    21001 tcagtactgc gggcagagcg agcacgttcg cgttgtgcat ccatttcttt ttcaaaacca
    21061 gcatgatcaa ctttcagtcc ttcatcttct gccacctctt ccgttaattc taccgggaag
    21121 ccatatgtgt cgtatagttt gaagatgtct tttcctgcta gagtcgtccc gttttcgttt
    21181 tttacttctt ggatcacttg atttaagatt tccaaacctt cattgattgt ttcattgaaa
    21241 cgttcttctt ctgtacggat aactttttca ataaattctt tttgttgaag tacttcagga
    21301 taataactta ccatgatttc cccaacaaca gggactaaac gatataagaa tgcttgttca
    21361 atacctaatt ttttcccatg catgacagca cgacgaagca aacggcgtaa gacataccct
    21421 ctcccttcat tcgatggcaa tgcaccgtcg ccaatagcaa atgaaagggc gcgaatatga
    21481 tctgcgatta ctttgtaaga aatatctgtt actgggtttt caccatattt tacttgtttg
    21541 cttagctttt ctacggcgtg gataataggc ataaacaagt ctgtctcaaa gttcgttgac
    21601 gcatcttgaa cgattgagac catgcgttcc aatcccatcc ccgtatcaat gtttttatga
    21661 ggaagtggtt caaatgtgtt atcaggctta tggttgaatt ctgaaaatac taagttccag
    21721 atttctaaat aacgttcatt ttctcctcct ggatagtttt caggatcgtc ttctgctaaa
    21781 tcgttataag cttctccacg gtcatagaaa atttccgtat ccggaccaca tgggcctgca
    21841 ccgatatccc agaagttgtc ttctacatca ataatgtgat ctagtggcag gccaacttct
    21901 tcatgccaga tgcgttttgc ttctgtatct tttggataaa ctgttacata aagtttttct
    21961 ggatcgaatg ccatccattt tggatcagtc aaaaattccc atgcccagtg gatggcttca
    22021 tttttgaaat agtcaccaat cgaaaaatta cccagcatct caaacatcgt atggtgccgt
    22081 gctgttttac cgacattttc gatatcattt gttcgaatag atttttgggc atttgtgatt
    22141 cttggatttt ctggcacaac tgaaccatca aaatattttt tcaatgttgc aacaccagag
    22201 ttgatccaca ataaggttgg atcgttcacg gggatcaaag acgcactagg ttcaatcgta
    22261 tgtcctttag attggaagaa atcaagaaac atttgtcgaa cttgtgcact acttaattgt
    22321 ttcatgtcat tacctgcttt ctataaatta aatacaaaaa aacaccatca cagccaagga
    22381 cgaaaactcg cggtaccacc ttgattgcaa tgatgtgtaa tcattacctc ttaaactcga
    22441 taacgctgag ctgcgtcggt atttctacca aaatagaaaa agggagcaat ttgattagtg
    22501 actattcttt tttcagccaa gaaagaattt tctgtgagtc ttaaaaaaca aatcatatcc
    22561 ttttcgtact taattagtat acgcattcta ttgatcactg tcaatatctc aatttttatt
    22621 ttcatttcct tttggtcata ctccacgatt tttggtacga taagaataaa ggaatcaata
    22681 catctctgct attcatgaca aggaggaata tgatgaaaaa aatagttgga gcggatgaat
    22741 ggatacgcgc agctagctgc tttttgagat atcaagtttt tgtattagaa caagggattc
    22801 ctgcagaact agaattcgac ggaaaagata tcccgcaaca aacctattat ctatttcttg
    22861 atcaaaaaag accagtagct actttgcgtt atgaaaagaa aacacctact tgcttcaagc
    22921 cggaacgatt ttgcgtagca aaagaatacc gaggaaaagg gttaggcggt cagttgcttg
    22981 aagctgctga aaaacgggcg gtttcagaag gttgtcagac tagttatgta gtagctgaaa
    23041 tgacggctgt agatttttat gaaaaacaag ggtatcatac ttgcacagaa ccttttattg
    23101 aagatgaagt actttgtatc ggaatgaaaa aaagtctagc ttaaaaacaa gagattgtga
    23161 cacgcctttt gtcacaacct cttgtttttt atttatgtga atttttctta tttttacgag
    23221 cagtacggct ttgctgtctg cgttcgattt tgcgttcttt tttatttttt tctgcaattg
    23281 cccaatcgat ttttttctta taaccaggct tgatcttttt ctttttcttc ttcacaagac
    23341 caatcagcgt gggatctaat acatcttttg atttttctcg tttggcacgt ctattacgat
    23401 cgtatgtttt gacgatttcc ccgtttttga tttcttttgg ttcaaatgag acgcccaatt
    23461 tttctatcgc atcaatggct tgctcatcat ttggcgaata taacgtaatg gccgttcctt
    23521 ttaagccatt acgtccagta cgtccgacac gatggataaa gaagtctaga tcttctggaa
    23581 tttctgcatt aataacatga gagacacctt cgatatcgat ccctctagca gctaaatcag
    23641 tcgctacaac atattgatac tccagattct gtacttgtcg catcactcgt tttctctctc
    23701 ttggggtgat gtctccatga atctttgcta ctttcaagcc ttggtttttc aagtaatcgg
    23761 caatttcatc cacatgctgt tttgtattgg caaatacgat tgctaaataa ggatgaccaa
    23821 ttgtcagcag ttgataaatc atttgattct catctttacc ttttgttgaa atcagccaat
    23881 tttcaatgtc ttcagaaata actgcttttg gtttgatttc ttcaatgatt ggattttcca
    23941 tgtattttcg taagaatgga cgaagttttt ctggaatagt ggcagaaaaa actaaaaact
    24001 gcagcttttc tggtaatcga cttgcaattt ggtctacttc atttaaaaaa cccatatcca
    24061 atgtcatatc cgcttcatcg acaacaaatg caaatgcggt gtgaatcttc agcgcttgtt
    24121 cattcatcat gtctaatatt ctaccaggcg tgcctactac aacctgtggc tgttggtgat
    24181 gcagacgatt taattgacgc tgtttgtctg taccgccgac aaaattcgag acacggatct
    24241 ccggtgtaga aaatttagcg atttgtgtag ctgcttcata gatctgtgca gctagttccc
    24301 tacttggagc tgtaataaca acttgtactt cctctttttc tgcattgatt ttttcaaaca
    24361 aaggcaacaa aaacgtatgt gtttttcctg taccagtttg agattgccca atgatatttt
    24421 ttccttttgc aattaaagga atcaaacgtt cctggacctc tgtgggctgt ttgaagccct
    24481 ttgcggttaa cgcttcattg ataaacggtt tgaagcggaa tatatcaaaa ttattcattc
    24541 gtgcacctct tctctttaaa ttatcacgac tttggcatta taacacagac aaagcgtttt
    24601 tgctcagcta atttcgagaa tcatcatcct ttgtttggcc aatcgtgcta acagtatatg
    24661 tgtgactagc tttttcataa aacagaatcc gtttttttct caaaaatgat agcaagatat
    24721 ttaaaataaa aataccttgt agtaacaaga caccgagaga aatgaatact gcactcatct
    24781 gcaacaccgt attcgttgtc gaattcatga tttgctgaag caactgcccc aaccagctta
    24841 aaccataaaa aagaaagtac agatacccgt agcggatcat caatgctttt aaagaaattc
    24901 gttcgcggtc ttcttccacc aaacggatac gcacaatttt tttgccaagt gtccatccat
    24961 tggttataaa cggtacaatc ataaagtata cccctacttc taataaaagc caccagatat
    25021 gagaagtaac tgcccctgaa tcgaacgcaa ttttaaagaa aatcgccgct accaaattaa
    25081 taagagataa aaatgtccag tcgatcaaat aagcgaccaa gcgtcgagtc aatgagacag
    25141 tctttccttt ttcataagaa agttcatcca ttcgtcttct tgtaggaagc ataaatgtaa
    25201 ataacggcgt aacgacaaaa ccaatgattc cacccaatgt atttgtcact aaatcattca
    25261 catcaaataa gcggtatggt cttgggtaga taaaataaag tccacttagc tgcgtcaatt
    25321 caaaaaatag agaaaggcag aaactagcaa tcatcgtttt cataaaagaa agccggaaat
    25381 aatatcttaa ataaatgcca aatggtatga gtaataaaac attaaaagct ggttccaaaa
    25441 atacactctg cttcattgcc ggcaggtagg tactaggttc agacaagacc aataccgtgt
    25501 tgtctctgaa atgttgccat gaagcaaata aatgcagctc catctttggc cctgtcattt
    25561 gggcaaccgt ctcctttgcc ggaagtggta agatcaccaa aaaatacgca cacaataaat
    25621 aaaaaacgaa agaatacaaa ataactgccc ttgttactag aaatgttcca tactttcgat
    25681 attcataaat aaaaaaaaca gatgagatcg ctaaggctaa aaatggaaaa acaatcagcg
    25741 caaaacgaat cggttcgata taacttgcca tatgactcct ccttgattct ttgtatctct
    25801 cttatcttag cagtcctttg ctgtttcaag caagtaacga ggctattatt tctcatttct
    25861 ttcaaagtaa agtaagataa acacaaaggg ggtgtcgaga tgctgaatat gtactttgtt
    25921 tttggcgtac ctattttttt actgttcctt tatgctacga tcgcttatgt aagaaaaaga
    25981 actacaattc attatttagg atttattctc ctgattattt cgggtttcat gctagtcttc
    26041 aatcttcaga cttggcagca agcacttctt gaaatggata aaatgactcc acatgctttg
    26101 tctaaagtgc ttggctaccc tgtctatctg atttggcttc ccattttcat ttctggttgc
    26161 ttggttttat taaatattta tcgcggcgta cgccgaatag ttcagttacg caaatcaaag
    26221 taaaagacag aaatcggttg agcccgattt ctgtctttta ctttgctatt tcatcgtaga
    26281 caaaacgaca tttacgtgat caaaattaac gagtactttt gacatttctt ccacgtactc
    26341 ctcatttaaa taaaatattt tgtttggcag agaagtgcag ttctaataaa attcgaatgt
    26401 tttagctatc tacgttttaa atagagataa atcgggatac cagcaagtgt caatactatc
    26461 cccatcgaag ctaaaaaggt ctgagtgata agcgtgcttg atacgataaa agtccctcca
    26521 atgattgcta ctagtggaat aaacggatac attggaactt tgtatggtcg aaacaaatca
    26581 ggttcttttt tacgaagaag aatgacacca acaaagacca tcgtataaaa aatccaaatg
    26641 acaaagatca gcatatccgt tagtgtatca aaaccaccga tcatcatcat gccaatagcg
    26701 atgactagct ctaagatacc tgcggctaca ggaactttag ttttatcatg aagtttagca
    26761 aagagcttgc taaaaggcag gttgttttct tttgccataa cataagggag acgcattcca
    26821 gtaagggtat agccattgat ggttccataa acagaaatca agataccaac tgtgaccagt
    26881 cttccaccga agccgccaaa aatcattttt gcaacgtcac ttgccgcatt gctgtttcct
    26941 gcgactccgt cgatagatgc ggttcttaag aacaccgcat ttactaataa ataaacgatc
    27001 atgattccga tgattcctaa agaaattgct tttggcaaat ctttcgccgg ctttttcaat
    27061 tctcccgaaa tatttcctac gtgtatccaa ccgtcgtatg cgaacatcgt tgctagcaag
    27121 cccgctccca aggctgagaa aaatgaaaga ttttctcctg cttggatcgg aaacagttgg
    27181 aagtctactc cttcttgtcg gaacaaaccg aaaatgacga tcacaaacaa aggaataagc
    27241 ttgcagacaa gcgtaatcga ctgaaaagcc cctccagctt tagaacctaa aaaattgatc
    27301 aacaggatcg atacagcagc tgtaacagct acgggaacaa tcattgactg ggaaagacca
    27361 aatagattga cgaactgtgt accgaaaatg atcgacaagg ctgctacatt tgctggaaaa
    27421 taaatcacga cttgcgccca gcccaataaa aaagctgctg tatttccgta aatccgttcg
    27481 atatatttaa tcatccctcc tgtttctgga atagcagctg ccagttcagc tcctgttagt
    27541 cctgcacaga cagaaatcat accaccgaga aaccaagaaa acatatgcaa actggcggat
    27601 cctgtaactt ctgctacgct tgcggcttta aaaaataccc ctgcaccgat cactgtgccc
    27661 ataacagtcg ataaagcagt aaaaaatccc atcgtacgtt ttaattcatt ttctattttt
    27721 acttctttta ctggcgtggc ttgatattct tccatccctt gtcccctttc ataccatagc
    27781 caatcccaaa agtataaaaa actttttgga tgtggttctt gttttatacc gtctctacca
    27841 atttttcttt cagtttttcc aacatatcct ttgtcatcac atctaagtcg tatttcggcg
    27901 aaaagcccca ttcctcttgc gcacaagaaa catctagtga atttggccaa gattctgcta
    27961 ttgactgacg tacaggatcc acatcataat ccatttcaaa atctggtatg attttttgaa
    28021 tagatgcttt gatctcttcc gggtcaaaag acatcgccga tatattgaag gcatttcgat
    28081 gaaccaatcg atcaggatct gcattcatga gttttataat ggcatcgatc gcatctggca
    28141 tatacatcat atccattgat gtaccttgat caatgaaact agtgtacttt ccttctttga
    28201 ttgctgaata ataaatatcg actgcgtagt ctgtcgtacc gcctcctggc aaggttgcat
    28261 aagaaatcaa tccagggaaa cggacaccac gagtatctac gccatatttc acataataat
    28321 aatcacatag tagttctcca gctaccttcg ttaccccata catggttgta ggacgctgaa
    28381 gagtatcttg tggggtatga tcttttggag tatttggacc aaacgcgccg attgaacttg
    28441 gcgtaaaaaa tttcagctgg aattctctag cgacttccaa tgcattgacc agcccagtca
    28501 cgttcaaatt ccaggctaat tgtggttttg cttctgcaac agctgacaaa agagcagcca
    28561 aatgaatcaa tgtgtccact tgatattgtt ctgctatgct gcgcattttt tcgtaatcca
    28621 ttacatctag gatctcaaag atatcgtttt tctcaatgac ttcaggcatg cgtatatctg
    28681 tcgcaatgac attttcactg cctagttctt gacgtagccg tgtgatcaac tctgaaccaa
    28741 tttgtcctaa acatcctgtc accaatactt ttttcatgat gatcctccct gatctttttt
    28801 actactatct tcagatgatt aacaatatat gcgtgaacat gttctctaat tttaaataga
    28861 cgaaaaaact aaatttagtt aaataatttc catttctttt cctatttttt gataaactgc
    28921 tactgcttca tcaagcattt ccctcgtatg ttccgcagtc ggcatatttc tgatcctgcc
    28981 tgtcccaaga ggcacagttg gataaacgat tggtttagca taaatcccat gttcgatcag
    29041 cttcttagag aaagtctgag tcactttttc gtctcccaaa atgactggag taataggtgt
    29101 ttcagacata ccgatgttat agcctacttt tttcaattct tctttaaaat aattagcatt
    29161 ttcccaaact ttttcgacaa gttccggatg ttcttgcatc aaagtaatcg aagccaatgc
    29221 agctgcagct gcacctggag ttagcgaagt agaaaatagg aatggccgac ttctagcttt
    29281 caaccagtcg attagtgttt tcgatccagc tacataaccc ccaacaacac ctatagcttt
    29341 cgataacgtg cccatttgca tatcgatttt gtcgcttaat ccaaaatgct tgacggttcc
    29401 tgcacctttt ccagttacgc ctgagccgtg cgcatcatcg acgtaggtga tcagtccata
    29461 tttttcaaca atcggaataa tttccggcaa tcgtgcaatg tctccatcca tggagaaaac
    29521 accatctgtg atatacatga tttttttata tttcttactt tctatcgctt cctttgcttt
    29581 tttctcaagg tctttcatat cttgatgttt aattcggatg atttttgctc cggataagcg
    29641 gcaaccgtca ataatagatg catggtttaa ttcatccgat aagatagcat cttcttttgt
    29701 cattactgca gagatagcgc ccatattgca gttgaatcca gattgaaagg cgattgctgc
    29761 ttctgtccct ttgaattctg cgattttttc ttctaattgc tgatggattt tcagcgaacc
    29821 attgatcgtt cggactgctc ctgctcctac accataagtt tcggttgctt caatacatgc
    29881 ttttttcaat tcttcgcgat tggcaaatcc aagataattg ttggatgcta gattgatcat
    29941 cttttttccg tctacgatga tacaagcacc attttcactt tctagtatgt cgatcgtatt
    30001 gtataacccc ttttctttca gttgatccaa ttctttctct aaatacattg ttagttcttt
    30061 ggaagacatt gtctccacct ccatattcat ctgtcttgca gaacatacca aacatatatc
    30121 gtgtcattca tctacaaagc aagtatgcta tatttttgat
//
LOCUS       DACDKL010000014        28038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDKL010000014 DACDKL010000000
VERSION     DACDKL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.014"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(205..1104)
                     /locus_tag="IV329_000517"
     CDS             complement(205..1104)
                     /locus_tag="IV329_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8939724.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="IV329_000518"
     CDS             complement(1114..1470)
                     /locus_tag="IV329_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8939725.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="IV329_000519"
     CDS             1825..2121
                     /locus_tag="IV329_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8939726.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="IV329_000520"
     CDS             complement(2765..3718)
                     /locus_tag="IV329_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8939727.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="IV329_000521"
     CDS             complement(3741..4496)
                     /locus_tag="IV329_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8939728.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="IV329_000522"
     CDS             complement(4493..5452)
                     /locus_tag="IV329_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8939729.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="IV329_000523"
     CDS             complement(5445..6395)
                     /locus_tag="IV329_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8939730.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="IV329_000524"
     CDS             6545..6997
                     /locus_tag="IV329_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8939731.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IV329_000525"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="IV329_000525"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8939732.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IV329_000526"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="IV329_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8939733.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="IV329_000527"
     CDS             complement(10549..11358)
                     /locus_tag="IV329_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8939734.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IV329_000528"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="IV329_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8939735.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="IV329_000529"
     CDS             complement(14968..15654)
                     /locus_tag="IV329_000529"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8939736.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="IV329_000530"
     CDS             complement(16002..17783)
                     /locus_tag="IV329_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8939737.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="IV329_000531"
     CDS             complement(17809..18723)
                     /locus_tag="IV329_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939738.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="IV329_000532"
     CDS             complement(18750..19712)
                     /locus_tag="IV329_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8939739.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="IV329_000533"
     CDS             complement(19712..20656)
                     /locus_tag="IV329_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939740.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="IV329_000534"
     CDS             complement(20657..21661)
                     /locus_tag="IV329_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939741.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="IV329_000535"
     CDS             complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="IV329_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8939742.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="IV329_000536"
     CDS             complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="IV329_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8939743.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23343..25379)
                     /gene="recG"
                     /locus_tag="IV329_000537"
     CDS             complement(23343..25379)
                     /gene="recG"
                     /locus_tag="IV329_000537"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8939744.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25496..27178)
                     /locus_tag="IV329_000538"
     CDS             complement(25496..27178)
                     /locus_tag="IV329_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8939745.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27230..27592)
                     /locus_tag="IV329_000539"
     CDS             complement(27230..27592)
                     /locus_tag="IV329_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8939746.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27862..>28038
                     /locus_tag="IV329_000540"
     CDS             27862..>28038
                     /locus_tag="IV329_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8939747.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKV"
BASE COUNT         7755 a         6072 c         4934 g         9277 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg ataaaaagct gcatgcagct
    21901 ttttatcctt ctttgctttt atttgaaatt atttaagatg gctgctgata taatctactg
    21961 cgccaccgac tgtttcaatt tgttcagcgt cttcatctga aatttccgta ccgaattcat
    22021 cttcaagctc taaaacgaat tccataatac taattgaatc agcttttaaa tcatctttga
    22081 tgttcaattc atccgtcact ttatcggtat ctatctcaaa atgatttgaa ataattttcg
    22141 ctactttgtt aaatacttct tcgcgagtca actttttcac ctccaagaat catacacttg
    22201 tcaattacct acaccatggc taattttact ggcatttttc aggaatgtcc accttttttt
    22261 caatggaacg gatcgcttca gatatccaaa tactattctt cgcttttttc aaattgttcg
    22321 actaattgtc caactacatc tgtctcaagc attgtgtgga tttggcggat cgtatagcga
    22381 acagcatcgg ggcctgttgc tccatgagtt ttaatgactg gtgctttcaa gccaaacaaa
    22441 acggcaccgc catgtttgga ataatccatt tctgctttca aagaacgtaa gccgtctttt
    22501 aaaagcatag cgcccatttt tcctttgatc ccttcattta gaatagcaga ttttaataga
    22561 ttcatcatat tcattgctgt tccttcaata gatttcaaaa cagcgttgcc tgtaaatcca
    22621 tctgtgacca ctacatcagc cacaccattg agtagctcac gagcttctac gtttccaata
    22681 aaattcaagc ttgtttcatt ctgaaggagt tcataggctt ttttcgtcac ttcactacct
    22741 tttgttgctt ctgttccgtt gtttaagagt gcgacacgag gacgtccgat acctcggact
    22801 ttacgtgcat aaaaagaacc taaaataccg tattgcaaca aatgttctgg tttgttttct
    22861 gcgttagcgc caagatccag catatcgaaa ccggcacctt ctttccctac tactggcaag
    22921 gtagacatca agccagggcg ttcgatattt ttgatacgtc ctacgatgaa caaccctgct
    22981 gctagtagcg cgcctgtatt ccctgctgaa aatacagcat ctgcttcacc gtttttcact
    23041 gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc gaattgcttt tactggctca
    23101 tcatcactat tgattttttc gtctgtatgg ataatcgtga tgtttttttc atttgttaag
    23161 tatttcttga tttctgcttc ttttccgtaa agtaagaatt ctacttctgg aaaatcttgc
    23221 tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt gatctccgcc cattgcatct
    23281 acggcaattt tcattgtttc cactcctttt tccgtcatca gtataaagta tagcacagtc
    23341 tgctaatcga aataacgatt atcctgattt tctatttgga ggctttgcgc taaaggctga
    23401 tactcaggaa gcatctgcca atcctttatt tgccaaaggg ctgcagcctc ttctctggct
    23461 acctccaaaa catttccatc cgtcaccaag tcagcaatcg caaattgagg cagaccagat
    23521 tgacggaagc caaatacttc accaggacca cgtaattcca gatctttttc gcttagtaca
    23581 aaaccattgg tggtctctgt catgattttc atccgttcca cacctagttc atttttagga
    23641 ttagcaacta agatacagta ggaagcatca cttccgcgtc caacacgtcc acgaagttgg
    23701 tgcagctgtg ccaatccaaa tcgatccgca tccatgatca gcatgacggt cgcgttcggc
    23761 acgtttaccc ctacttcaat tacagtagtc gaaactaata tctgcatctg gttgtctttg
    23821 aaggcttcca tgattacttc tttttcctga ttcttcattt taccatgtaa caagccaact
    23881 tcatattttg gtgcaaatag ttcacgaagt ttttcataga tttccactgc attttttaca
    23941 tctaatgctt ctgattcttc aatcagcgga cagatgacat acatttgatg tcctcttgcc
    24001 agttcttttt tcgtccaatc cagtacactg tccaattgag gtgttcttac ccagcgtgtt
    24061 tcgatcggga tacgtccagc tggaagttca tcaatgatag atacatccat ctcaccatat
    24121 gctgtaatag ctaatgttct aggaatcgga gtcgctgtca taaataagac atctggtcgc
    24181 cagccttttt cacgaagtac tttccgctgg ttcacgccaa aacggtgctg ctcatccgta
    24241 ataactagac ctaaattctg aaattcgact ccatcttgga tcaatgcatg tgtgccgatc
    24301 acgacgttaa tttccccttg ctctagtttt tcaaggagct cacgtcgttc ttttgctttc
    24361 gttgaccccg taatcaatgc gatggttact tcattgggat caaacagctg ttgcaagctt
    24421 tccatatgtt gttgtgccaa gatttctgta ggcaccatca acgcaccttg gaaacctgct
    24481 gtaacggtag cgtataacgc aatcgcggca accactgttt ttccgctacc tacatctccc
    24541 tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc gacagatttc attcgttaca
    24601 cgtttttgtg catttgtcag ttcaaaaggt agcttttgag taaattgttt caatttttgg
    24661 acatcataaa ggatctccaa accattcgtt tctgcttttt ctgctttttt caatccttga
    24721 agacgcattt gaaataaaaa gaactcttca aaaacgacac gtcgtttggc ttgatggttt
    24781 tcttcgtggt cttttggaaa atgcatactt cttacagcag aagctcggtc aagcagtcga
    24841 tatttctcca caaggttgtt tggcagattt tcttcaatca atgtgccgta ttcttgaaac
    24901 ccttttttga ttagatcaac taatgtcgtt tgacgaattg atttgttgac gtgatatatc
    24961 ggcgaaaagt catccattgt ttgagaacct aaaatcttca tcccgttcaa ggctttacgc
    25021 tttgcatccc atttaccgta tacggcgatt tcctccgata aaatgacttt ttcttttaga
    25081 taaggttgat taaaaaaaga aacgttgaac acatcatgtt cttgcatcat acgaaattgc
    25141 aatcggcttt ttttataacc aaatcgattc atcacaggag gcgagactac gattcctttc
    25201 aaagtaacct tttcttgatc ctggatctcg tttagatttc tttcctgaat atcttcgtag
    25261 cgaaaaggat aataagtcag taagtcttca atcgtttgga tgcccaagct ggcaagacta
    25321 gctgctcttt tttctccgac tcctgccagc atagtgattg gatcatctaa agaacgcata
    25381 aatttttcct ccctccttta acaaaaaaag gacttggtca gcgatcatac ctgatcacta
    25441 accgaaggcc ttttatgaat gaatactgtg ttagaaagat attctgacac agaaattatt
    25501 cagcagaaaa tagataagga tagacaggct gatctccttg gtggatctcg atttccaaat
    25561 caggatgttt ttctgttagt ttctcagcaa attcatttgc ttgttcaact gttccttcat
    25621 cacctgccaa gatcgttacg atctctgtgt cctcattgat catttgttct agtgtactta
    25681 acgctgttgc tgtgatatct gggttagaga tcaggatttt tccatcgatc atgcccaaaa
    25741 agtctccttc tttgatctct acaccgtcaa ttgaggtatc tctgatcgcg tgagtgactt
    25801 gaccgctcac aacgctagac aacatttctg tcatcgtttc tttgttttct tctaaagaag
    25861 cttgatcatt gaatgaaagc atagctgtca atccttggga aatcgttctt gaaggaacaa
    25921 ctgctactgg gatatctgct acttctgctg cttgatcagc tgccatgaag atgttcttat
    25981 tgtttggcaa gacaatcact ttatctgcgt ttacttcttg aatcgcttta acaatgtctt
    26041 ccgtacttgg attcattgtt tgtccaccac tgataacata attagcgcct aaactttcaa
    26101 acagctcttg gacgccttta ccagcagcga tcgctacgat accatacggg atacgtggta
    26161 tttctttttt cgcaaactcg ttcacttctt catcgtgttc caaaatcgtc tcatgctgca
    26221 agcgcatgtt gtcgactttg attttgacta atgaaccgaa tttttgtcca taattcaaga
    26281 cttctcctgg gttttctgta tgcacgtgga ctttgatgat ctcgtcatca ttgaccacaa
    26341 gtaaagaatc tcctatacca tctaaatagt tacggaattc ttcgtaatcg aatgtactat
    26401 cgactgtagg tccttctccg atttttacca tgatttctgt acagtaacca aaatggatat
    26461 cttctgtcgc catttgacct tggacactgc gatgatgttc tgcattgacc atatcatcca
    26521 tttctgctgg agaaggttca tatgtttcat ccgcttggaa ttctccattc aatgcactta
    26581 aaaagccctc ataaacaaac agcagtcctt gccctccact gtctacgaca ccgacttctt
    26641 ttaggactgg aagcaagtct ggagttttag ctaaagcacg ctttgctcct tttacaacag
    26701 ctgtcattac ttcaacgaca tcatcagtac tttttgcttt cttttctcca gctttcgctg
    26761 cttctctagc tactgtcaaa atcgttcctt ctactggttt cattactgct ttatacgcag
    26821 tttcaactcc atgagtgaag gctgcagcaa gatcagaagc atttaatgag acaacatctg
    26881 gaatttgctt agaaaatcca cgaaacaatt gagacaaaat aacgccggaa tttccgcgcg
    26941 cacccatcag caatcctttt gaaagcattg ctgctaattc tcctactttt tctgaggttg
    27001 aatctactac tgccttagct ccactagtca tagatagatt catatttgtt cccgtatcgc
    27061 catctggcac agggaataca tttaatgaat tgacgtattc tgcatttact tgcagacgat
    27121 tcgcacctgc ctggaccatt tcctggaact ggcttgcact tatttctgtt acactcacct
    27181 agataatcct ccttcagatc gactgctgag ttaccagcca gtctcctgcc tagtctggca
    27241 gtacacgaac accttgtaca aagacattaa ctgaattggc agttacgcct aataatgcct
    27301 ctagattgta tttcactttt tcttggacgt tgcgggaaac ttcagagatt ttcgtaccat
    27361 agctgataat cgtataaaca tctacagcaa taccattatc ttcttggcgg acaactactc
    27421 ctcttgagta attttcttta cgcaaaatct cagtaatatt atctttaatt tgatttttgc
    27481 tggccattcc tacaacaccg aacacatctg tcgcggcgcc accaacaact gttgcaatta
    27541 catcattggt aatttcaatg gttccagctg acgtttgaat ttttacagcc atgaatgagc
    27601 ctccttaacg ggtaaaaaac tcccattttt cttctatcat attttatcat agcgcaactt
    27661 gaaataaaag gcagttaccc aaagtactca aaaaccttgc aaaatttcag gtataacctt
    27721 gtttaattta caattattct tctcaattgt caattgtatc tatgaaaatg tacttgcaaa
    27781 ttcccattct atatgatatg ttagtctggt atgagctgaa ttattctatt gctccaaatc
    27841 aaggcaaagg aggaaattac catggcaaaa gtttgttact ttacaggtcg caagacaaaa
    27901 agtggaaaca accgttcaca cgctatgaac tcaacaaaac gtactgttaa acctaactta
    27961 caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag tttgggtgtc aactcgtgct
    28021 ttgaaatcag gaaaagtt
//
LOCUS       DACDKL010000015        27927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDKL010000015 DACDKL010000000
VERSION     DACDKL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.015"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IV329_000541"
     CDS             25..696
                     /locus_tag="IV329_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8939748.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IV329_000542"
     CDS             755..1195
                     /locus_tag="IV329_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939749.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IV329_000543"
     CDS             1207..1875
                     /locus_tag="IV329_000543"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8939750.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IV329_000544"
     CDS             1939..2562
                     /locus_tag="IV329_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8939751.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IV329_000545"
     CDS             2618..3004
                     /locus_tag="IV329_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8939752.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IV329_000546"
     CDS             3217..4608
                     /locus_tag="IV329_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8939753.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="IV329_000547"
     CDS             5219..5587
                     /locus_tag="IV329_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8939754.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="IV329_000548"
     CDS             5767..6708
                     /locus_tag="IV329_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8939755.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="IV329_000549"
     CDS             6777..7508
                     /locus_tag="IV329_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8939756.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="IV329_000550"
     CDS             7652..9832
                     /locus_tag="IV329_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8939757.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="IV329_000551"
     CDS             9852..10334
                     /locus_tag="IV329_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8939758.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="IV329_000552"
     CDS             10350..11111
                     /locus_tag="IV329_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8939759.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="IV329_000553"
     CDS             11242..12576
                     /locus_tag="IV329_000553"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8939760.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="IV329_000554"
     CDS             12883..13368
                     /locus_tag="IV329_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8939761.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="IV329_000555"
     CDS             13361..14044
                     /locus_tag="IV329_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8939762.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="IV329_000556"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="IV329_000556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8939763.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="IV329_000557"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="IV329_000557"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8939764.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="IV329_000558"
     CDS             17660..18289
                     /locus_tag="IV329_000558"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8939765.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="IV329_000559"
     CDS             18341..18673
                     /locus_tag="IV329_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8939766.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="IV329_000560"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="IV329_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8939767.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="IV329_000561"
     CDS             19499..20872
                     /locus_tag="IV329_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8939768.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="IV329_000562"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="IV329_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8939769.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="IV329_000563"
     CDS             21927..22367
                     /locus_tag="IV329_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8939770.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="IV329_000564"
     CDS             22511..23212
                     /locus_tag="IV329_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8939771.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="IV329_000565"
     CDS             23209..24330
                     /locus_tag="IV329_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8939772.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="IV329_000566"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="IV329_000566"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8939773.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="IV329_000567"
     CDS             25709..26617
                     /locus_tag="IV329_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8939774.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="IV329_000568"
     CDS             26652..26924
                     /locus_tag="IV329_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8939775.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..>27927
                     /locus_tag="IV329_000569"
     CDS             26935..>27927
                     /locus_tag="IV329_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8939776.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRV"
BASE COUNT         9374 a         4833 c         6093 g         7627 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggtagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtg
//
LOCUS       DACDKL010000016        27851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDKL010000016 DACDKL010000000
VERSION     DACDKL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.016"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV329_000570"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IV329_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8939777.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV329_000571"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IV329_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8939778.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV329_000572"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IV329_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8939779.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IV329_000573"
     CDS             1642..2274
                     /locus_tag="IV329_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8939780.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IV329_000574"
     CDS             complement(2276..3181)
                     /locus_tag="IV329_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8939781.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IV329_000575"
     CDS             complement(3266..3919)
                     /locus_tag="IV329_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8939782.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IV329_000576"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IV329_000576"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8939783.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IV329_000577"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IV329_000577"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8939784.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IV329_000578"
     CDS             6168..7253
                     /locus_tag="IV329_000578"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8939785.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IV329_000579"
     CDS             7250..8314
                     /locus_tag="IV329_000579"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8939786.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IV329_000580"
     CDS             complement(8563..9366)
                     /locus_tag="IV329_000580"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8939787.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV329_000581"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IV329_000581"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8939788.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IV329_000582"
     CDS             complement(10244..11251)
                     /locus_tag="IV329_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8939789.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IV329_000583"
     CDS             complement(11258..11902)
                     /locus_tag="IV329_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8939790.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IV329_000584"
     CDS             complement(12002..12760)
                     /locus_tag="IV329_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8939791.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV329_000585"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IV329_000585"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8939792.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV329_000586"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IV329_000586"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8939793.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV329_000587"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IV329_000587"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8939794.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV329_000588"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IV329_000588"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8939795.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV329_000589"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IV329_000589"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8939796.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IV329_000590"
     CDS             18158..19027
                     /locus_tag="IV329_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8939797.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IV329_000591"
     CDS             complement(19153..20052)
                     /locus_tag="IV329_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8939798.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IV329_000592"
     CDS             complement(20217..21041)
                     /locus_tag="IV329_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8939799.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IV329_000593"
     CDS             complement(21052..22347)
                     /locus_tag="IV329_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8939800.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IV329_000594"
     CDS             complement(22363..22842)
                     /locus_tag="IV329_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8939801.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IV329_000595"
     CDS             complement(22848..23876)
                     /locus_tag="IV329_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8939802.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IV329_000596"
     CDS             complement(23893..24999)
                     /locus_tag="IV329_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8939803.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV329_000597"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IV329_000597"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8939804.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7850 a         6122 c         5034 g         8845 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt t
//
LOCUS       DACDKL010000017        27682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDKL010000017 DACDKL010000000
VERSION     DACDKL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.017"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            142..1908
                     /locus_tag="IV329_000598"
     CDS             142..1908
                     /locus_tag="IV329_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8939805.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2010..3269
                     /locus_tag="IV329_000599"
     CDS             2010..3269
                     /locus_tag="IV329_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8939806.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3416..4717
                     /locus_tag="IV329_000600"
     CDS             3416..4717
                     /locus_tag="IV329_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939807.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4720..5571
                     /locus_tag="IV329_000601"
     CDS             4720..5571
                     /locus_tag="IV329_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939808.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5584..6399
                     /locus_tag="IV329_000602"
     CDS             5584..6399
                     /locus_tag="IV329_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8939809.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6550..7716
                     /locus_tag="IV329_000603"
     CDS             6550..7716
                     /locus_tag="IV329_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8939810.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7946..8593
                     /locus_tag="IV329_000604"
     CDS             7946..8593
                     /locus_tag="IV329_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8939811.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8677..9621
                     /locus_tag="IV329_000605"
     CDS             8677..9621
                     /locus_tag="IV329_000605"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8939812.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9756..11207
                     /locus_tag="IV329_000606"
     CDS             9756..11207
                     /locus_tag="IV329_000606"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8939813.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11228..12064
                     /locus_tag="IV329_000607"
     CDS             11228..12064
                     /locus_tag="IV329_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8939814.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12064..12723
                     /locus_tag="IV329_000608"
     CDS             12064..12723
                     /locus_tag="IV329_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8939815.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12849..13538
                     /locus_tag="IV329_000609"
     CDS             12849..13538
                     /locus_tag="IV329_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939816.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13544..14500
                     /locus_tag="IV329_000610"
     CDS             13544..14500
                     /locus_tag="IV329_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8939817.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14544..15056
                     /locus_tag="IV329_000611"
     CDS             14544..15056
                     /locus_tag="IV329_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939818.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15312..15860)
                     /locus_tag="IV329_000612"
     CDS             complement(15312..15860)
                     /locus_tag="IV329_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8939819.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKCGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15949..17130)
                     /locus_tag="IV329_000613"
     CDS             complement(15949..17130)
                     /locus_tag="IV329_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8939820.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17366..18874)
                     /locus_tag="IV329_000614"
     CDS             complement(17366..18874)
                     /locus_tag="IV329_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8939821.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19033..19224
                     /locus_tag="IV329_000615"
     CDS             19033..19224
                     /locus_tag="IV329_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939822.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19427..20344
                     /locus_tag="IV329_000616"
     CDS             19427..20344
                     /locus_tag="IV329_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8939823.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20337..21410
                     /locus_tag="IV329_000617"
     CDS             20337..21410
                     /locus_tag="IV329_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939824.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21558..22076
                     /locus_tag="IV329_000618"
     CDS             21558..22076
                     /locus_tag="IV329_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939825.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22057..22449
                     /locus_tag="IV329_000619"
     CDS             22057..22449
                     /locus_tag="IV329_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8939826.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22525..23460
                     /locus_tag="IV329_000620"
     CDS             22525..23460
                     /locus_tag="IV329_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8939827.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23593..24372
                     /locus_tag="IV329_000621"
     CDS             23593..24372
                     /locus_tag="IV329_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8939828.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24365..24682
                     /locus_tag="IV329_000622"
     CDS             24365..24682
                     /locus_tag="IV329_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8939829.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24926..26536
                     /locus_tag="IV329_000623"
     CDS             24926..26536
                     /locus_tag="IV329_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8939830.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26693..27541
                     /locus_tag="IV329_000624"
     CDS             26693..27541
                     /locus_tag="IV329_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8939831.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8954 a         4895 c         5560 g         8273 t
ORIGIN      
        1 taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg
       61 ataacatttt tgaaagcgtt gtcttttgtt tctctatcac ttatagtgat gacgtcatat
      121 taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc ggaaagtgaa
      181 tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag gaaaaatgat
      241 atcaaaaaag tatatgtgct ttgtggagat ccatatacca taacaactga aaaatggtat
      301 caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt
      361 gaagtgacga gcgaaacaag gcgcttacag tatgcgttcc atgttgttgg ggaagataac
      421 acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt gattactgaa
      481 ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac tattccagaa
      541 tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa
      601 gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg agaagacttc
      661 ttcgggggag atttgcaagg tgtgagggat catttggatt acttgacgga ccttggcgtc
      721 aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata cgatacgatt
      781 gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa cttgattgat
      841 gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca tatgggtgtc
      901 cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta taaagactgg
      961 ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc agaaactgct
     1021 gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact aaatacagca
     1081 aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa agaattcgat
     1141 attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg gaaaaaattc
     1201 agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat ttggcattcc
     1261 tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc attcacagat
     1321 tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt cagtggaatg
     1381 aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa cttattagat
     1441 tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt gatgaagtct
     1501 gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg gacagaaata
     1561 gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga gaaggaggaa
     1621 caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa acagttgtct
     1681 aagattattt ctgaaggttc tactcagtgg ctaatagtat atgatcgtga agataagctc
     1741 tatttcacac gggaactaga gggacagatc atctatgttt actttaatca gtcaaaagaa
     1801 ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca gttgttagaa
     1861 gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa actgttaccg
     1921 gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa gggtgtaaca
     1981 gcacgttgaa tacacaggag gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt
     2041 tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat ggtaactctt
     2101 cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat tctggatata
     2161 aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc
     2221 tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg cctgctggaa
     2281 agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga cagcaaggac
     2341 atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac aaagcccaag
     2401 tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt gttttgtact
     2461 ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag aatctatcca
     2521 aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt ttagctaaat
     2581 ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat gtatttggtg
     2641 atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta gaaggaatct
     2701 cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg
     2761 caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc attgacggtc
     2821 catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc aaaattccta
     2881 ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca
     2941 attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa
     3001 atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa gctagggaaa
     3061 cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat gaaacagctc
     3121 aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa aacttgatgt
     3181 tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa
     3241 tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt tttataaaaa
     3301 atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat cttgctcttg
     3361 ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca ttcagatgca
     3421 gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg
     3481 gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg ccatattcgt
     3541 cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat tggttacttt
     3601 gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca ctttgcaatt
     3661 catcattaca atcattttct tgatttttta cggtttgaat cttttagacg cttatcgagt
     3721 agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag tcatacataa
     3781 tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc tcatgatttt
     3841 tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt atgacttcaa
     3901 tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt ttagtatctt
     3961 ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg tgatttggac
     4021 tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg ttgctcacca
     4081 gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat gggcggtccc
     4141 agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag gtgccatcaa
     4201 tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg caatctcttg
     4261 gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct ggttaggatt
     4321 tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag acttatatga
     4381 agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc
     4441 tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt tcaataattt
     4501 ctccatgatc tatctgttta acaatggtgg tcccggaagc gtaggcggag gagctggttc
     4561 tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc cgcagtattc
     4621 tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt cattgctggt
     4681 atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt
     4741 gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt aatactgtca
     4801 gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc aggaaatatt
     4861 gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac tcggctattc
     4921 agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt cctaacaatg
     4981 atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta tcgttttatc
     5041 ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac tatggcagca
     5101 ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta ctggttcttg
     5161 acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa aggatacttt
     5221 gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg gcatttcagg
     5281 atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca agcattatgg
     5341 gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg aacacctgaa
     5401 aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa ccaaagagtc
     5461 tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt attcttcttt
     5521 ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata ggagcagagt
     5581 atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac tcaagtgttt
     5641 gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt tttagtttcc
     5701 ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat tccattagaa
     5761 caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt
     5821 gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac ccctgaaatt
     5881 ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt tattgatttt
     5941 gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg ctatacgatg
     6001 aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt ccaacgattt
     6061 ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta tagtttaagt
     6121 cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc ctttttcttg
     6181 tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag tgcaaatttg
     6241 atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt tatccacttt
     6301 gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct tttatgggtt
     6361 tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc gatttatgcg
     6421 acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta
     6481 aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt ttttttaagg
     6541 aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc tttctcgctc
     6601 cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac ggacactcct
     6661 ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac aagtaacgct
     6721 tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg aagtatccaa
     6781 gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa agattatcta
     6841 aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg
     6901 cattatgact taggtaacga cttttataaa ctttggctag atccaacttt gacttattct
     6961 tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa taaagtccat
     7021 catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc
     7081 tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac tgggatcaca
     7141 ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca tttagaagat
     7201 aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc
     7261 acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg
     7321 gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag ccgacaacaa
     7381 ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta tgtccctggt
     7441 gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga tttggaaagc
     7501 ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca tcaagtccaa
     7561 gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct atatttacaa
     7621 gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt gcttgttcaa
     7681 cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta ctgtttaaag
     7741 actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt tgtctaactt
     7801 ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata aaagtttggt
     7861 tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa tagaatacta
     7921 ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta gtttatttgc
     7981 agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc
     8041 ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag caagcacatc
     8101 ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata tcaaagtgtc
     8161 tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa ttactagttt
     8221 ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg atgatcaaaa
     8281 agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga atccagaaac
     8341 tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa
     8401 attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc
     8461 aacagattgg gatttggata aagatttagg tactacttat tgggaagtca aagtaaaaga
     8521 cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga
     8581 acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta gtcatgatac
     8641 actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac gacctttgcc
     8701 aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat gactacccag
     8761 gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag ctgtacgatc
     8821 aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg gagtatcaat
     8881 tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct tgcgtatgaa
     8941 aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat gagtgtccag
     9001 gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat cactgaatta
     9061 aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt tgaggccact
     9121 tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat caaattgcct
     9181 ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca gtttccattg
     9241 acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac agaacaagaa
     9301 gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta tatcaagccg
     9361 actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat ccaaatcatt
     9421 ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg tggcgcaagc
     9481 atgttacaga ttggtacaga gcttcataaa gaagggccgg aaattttttc tagaatcata
     9541 aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga atttaaaggc
     9601 aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc gtcttttttt
     9661 ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt tagacgaact
     9721 tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa
     9781 aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata cagaaaaaaa
     9841 taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt
     9901 cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc ccatttcctt
     9961 taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct cgtttatttg
    10021 aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg ataggtctag
    10081 ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca gacccagtca
    10141 tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga tcaagtggag
    10201 gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt gatggcggtg
    10261 gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct tcgcgaggga
    10321 cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt gatttcggct
    10381 tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt atccattccg
    10441 gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa aaacggttga
    10501 agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag gcagtacttg
    10561 ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca gagattcctt
    10621 acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc gcagaaacag
    10681 cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg
    10741 aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct acttacatcg
    10801 attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat caagaatacg
    10861 atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa gatttgcaaa
    10921 gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta gaaggtttag
    10981 acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca cagttggcta
    11041 atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa aacatgcctc
    11101 taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa atcggctatc
    11161 tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag aaacaaggag
    11221 aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt ttattttaag
    11281 cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg tagaaaaaaa
    11341 gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt ataatgatca
    11401 aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac ggttaaatgt
    11461 caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt caaaaagaga
    11521 atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa aattctccct
    11581 ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag atgattcagg
    11641 aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca atactagcta
    11701 tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg ccacgaatga
    11761 tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg attattatct
    11821 gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg agaacgttta
    11881 tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc tgaaaaagaa
    11941 aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa tatctgaaaa
    12001 atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt cggtgagtga
    12061 gtgatgtact ttcccctaat tgatttttcc ctcatgtgga cttcacttcc tttcgtactg
    12121 caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg aaatttggta
    12181 gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt atttattcgt
    12241 ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct gttatatttc
    12301 gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac gattaccagc
    12361 agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc aggtcagtgg
    12421 gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat cgttttgccg
    12481 caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct agtgaaaggt
    12541 acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa gatcataggc
    12601 ggtcggacat tcgattattt ttctctctat attttagttg cactgattta ttgggtcatt
    12661 tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatcca tcagtcaatc
    12721 tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt ttcttgtata
    12781 ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata tgttcggagg
    12841 tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac caatattgca
    12901 tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag
    12961 atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc tatgagctga
    13021 aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga cattataatt
    13081 attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct acaggaatag
    13141 gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct ggtacatttc
    13201 tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc
    13261 gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa
    13321 aatattttcc aaaagatcaa ttgtacaaag aattgaaaag acgttcagaa aatcaagatc
    13381 cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt caggcaatca
    13441 atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg aggcaacaac
    13501 agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact atccttattt
    13561 aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag gattgctctc
    13621 caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta aaaatgtgat
    13681 cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc aagtaggaca
    13741 attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata gctctgtcgt
    13801 ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac ctccagcgtt
    13861 tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac agttggcagg
    13921 agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc tgcccgaaaa
    13981 caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt atgcagataa
    14041 aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat atattggatt
    14101 ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta gccgagcgct
    14161 tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac attttgtctc
    14221 tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag gagataaaat
    14281 gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa tgattgaatt
    14341 attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa agtggtcact
    14401 tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt ggcttaaata
    14461 ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg taaatgaaaa
    14521 aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa gaagtagaag
    14581 aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat
    14641 ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa
    14701 gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg
    14761 atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct tatttatcga
    14821 ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag
    14881 aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc gatacttatc
    14941 cagctaatga accgatggaa aagaccatct attctgctgg attcacttat caagggatga
    15001 ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc aattgactat
    15061 aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat catatgatag
    15121 ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc
    15181 ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc tgaaactccc
    15241 ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct cggcttatcc tacgagtatt
    15301 attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc ttggaacgct
    15361 ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa ctgtgctaaa
    15421 tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc acgttttttc
    15481 aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac taccgtcccg
    15541 aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca atttcctact
    15601 gtcagtttct ttttaatttc tgctttactg gaaccacatt tatgttgagc atattggtcc
    15661 cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga aacaaaataa
    15721 taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt
    15781 tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga aggaccgata
    15841 aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt gaaaaagctt
    15901 tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatttt ataccagcaa
    15961 gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc
    16021 gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac cagaaatgat
    16081 cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg ctgttgtacg
    16141 ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt catttgtaac
    16201 cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga ttccgctgat
    16261 gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc cgaatatccc
    16321 gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca tagcagcaac
    16381 cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga aaaatgtctg
    16441 ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat agggatttac
    16501 aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga tcacatattt
    16561 agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag acatagccga
    16621 tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt ttatggaaat
    16681 aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt ccaatttgcc
    16741 catcccgttg attttactta atcctagacc aacagctttc ataaataatg gaagaatctt
    16801 gatatagcgt agaataccga taatagcaga aataaataca attggcatca atacggaaag
    16861 aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa caccttgccc
    16921 agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg ctgtaccaaa
    16981 agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca caattagtgg
    17041 tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa
    17101 taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa ttccgtttta
    17161 ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta cttttttaga
    17221 gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg tttaaaaagt
    17281 gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa tagtttcgtt
    17341 ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa gggtagacag
    17401 gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt ttattgacca
    17461 tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa gcaacttgag
    17521 cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg atgcttagtg
    17581 catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca agaactaatg
    17641 gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca tttaagataa
    17701 ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact tcatctggta
    17761 attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga atcttatctg
    17821 catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg acttcttcca
    17881 taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg aagtcgcatt
    17941 ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca acggtatcat
    18001 ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga taaacagatt
    18061 ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca acatcccgat
    18121 tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc atattgacat
    18181 cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc aacggaacat
    18241 tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt tggctttcat
    18301 gatacagttg gttcaagcaa gtagcgagct ttccacttcc tgggccagga gctgtcacta
    18361 caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct tctgagacga
    18421 ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc ttgatgtttc
    18481 gtctttctag tttattgata aagactttcg tggcaggctg tccactatag cgagtgataa
    18541 cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc aagatatcca
    18601 tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct ccagcataga
    18661 cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt ttcgcatctt
    18721 cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactaacttc ccaccaaatt
    18781 ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac tgctcttcaa
    18841 tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa gtactaaagc
    18901 attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca aaaaaacaaa
    18961 gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag tttttgataa
    19021 ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca ctatatccgt
    19081 tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca attgacgttt
    19141 acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat tcatttcaca
    19201 acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc gattctgtcc
    19261 tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg
    19321 ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat ggtactattg
    19381 aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga aagagccaga
    19441 tacaaatgca aattatgagc cagacgaact agaggagacg aacgcggatc aggatacagg
    19501 acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc tttcccaatt
    19561 attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta
    19621 ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg aaaatcacga
    19681 gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat ttccttttat
    19741 ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca cttattacca
    19801 tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt tgcctaagat
    19861 cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca aaaaaatcaa
    19921 tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag atgaacaaat
    19981 aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct tgaatcgtaa
    20041 aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat ctaatgaaaa
    20101 attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt
    20161 gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag aacaaaggca
    20221 aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg aattcttgat
    20281 gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt ccgagtatga
    20341 atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat catgaagaaa
    20401 cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa gaaatcccta
    20461 tgctagagga aacaaaaata catgaaaaag aaaaagtaac tccgacgaat acgaatactg
    20521 cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa ggaaaagagc
    20581 aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta gcggatttac
    20641 gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca tatgatcgtt
    20701 ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc gaatacttca
    20761 cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat tcagcttatg
    20821 acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa aacgaattag
    20881 tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg atgagtctcc
    20941 taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg ctttctttga
    21001 tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata ttcaatcaca
    21061 aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat cagacattac
    21121 tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg acgaatgaag
    21181 tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg gaaaagatca
    21241 acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata acaacaaacg
    21301 aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct gtcattgatt
    21361 tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga ttggataagt
    21421 tggattacca gatgtcattc agataattct gttccaaagg acttgcggca gttatccaga
    21481 ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt gctgatgaag
    21541 gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta ttgtctgctg
    21601 cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca atgactgcta
    21661 tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc atgagtcttt
    21721 ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc caatggatca
    21781 gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga
    21841 atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga ataggtatga
    21901 ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac cttcaagggg
    21961 tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt tttctgccag
    22021 tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat ttatagtttg
    22081 cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta tacacaatcg
    22141 gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa
    22201 aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt
    22261 attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc gcttgaaggg
    22321 cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg gaaaagtgct
    22381 actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt caatttgtct
    22441 aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt cagtgagttc
    22501 agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat gttttccgta
    22561 attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg ctatgcaatc
    22621 agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg cagcaatcag
    22681 attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt gcatcaattt
    22741 ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct tttcttcggc
    22801 ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc ccatgaagtg
    22861 ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga aggaagttcg
    22921 ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc
    22981 tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga cttgtgtgct
    23041 gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac cgggattgct
    23101 gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc cgccattttc
    23161 ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag cgatttatca
    23221 aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat aaaaacagtt
    23281 catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg taacaaagag
    23341 aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac
    23401 aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc tcgtaaataa
    23461 aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa
    23521 cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt tgtaattaaa
    23581 ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg
    23641 atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt gttgcaaaaa
    23701 gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc cggtggcgcg
    23761 cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac attattgatg
    23821 gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa
    23881 aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga aaactatgcg
    23941 gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc tttatatgtc
    24001 aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt tttcggctcg
    24061 gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt cattttcatg
    24121 tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc aggcgtatta
    24181 ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc aacagtcatt
    24241 gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc taagaaacta
    24301 agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca ggaggccaat
    24361 caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg tggcatatat
    24421 cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct ggttcagtga
    24481 ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag atgttttcat
    24541 tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga ttctagtagc
    24601 aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt
    24661 ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat tgcgacataa
    24721 gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga tgcaagttta
    24781 ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt tattttagga
    24841 attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat gtttatttta
    24901 tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa cattttggct
    24961 aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc cctggctaag
    25021 tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg ttggctatct
    25081 tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa
    25141 agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt ttagaagtac
    25201 gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc
    25261 tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag ctatcagaag
    25321 tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat atttccaagg
    25381 aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg tgcgagtgat
    25441 cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca tattagaagc
    25501 tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta tccatatgta
    25561 tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt ccagccgtta
    25621 tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac taccgtttat
    25681 ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga tgaaaccact
    25741 gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat tcagccgtgc
    25801 ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa cagtagctat
    25861 aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac
    25921 agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga ctggaatgtc
    25981 tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa gtgtcggata
    26041 tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc tatctacgat
    26101 gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag caattatcct
    26161 gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga tgagcttatc
    26221 aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt gcaaagaata
    26281 ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc gctccaccct
    26341 gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga
    26401 atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg gcttagtatt
    26461 tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag
    26521 cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg
    26581 tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta atcttactta
    26641 tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac acatgaaact
    26701 tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc atcccgactt
    26761 attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag gaatcgatat
    26821 cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta ctacttcaca
    26881 acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag ggtatgacac
    26941 gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat tatttgcgat
    27001 gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca ctagtatgcc
    27061 gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt cccttgatga
    27121 aatacttgca catatcgatc gaatccgaga taatacctat gcttatctga ttgtagatga
    27181 tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga ttggcagctt
    27241 attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat tcgaaaaaat
    27301 ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa gaaataacga
    27361 aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag tagcgattgc
    27421 ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg
    27481 accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt cagcgcaata
    27541 attcctttta ttcaaaaaac ataccaaaaa tattggctct ttgtcaaatg atgttggtaa
    27601 agagaaatct tagaataatt tggacagaac agaaagaaga aaacctcttt ctgttctgtt
    27661 tatttggtta aaaatgtttt gc
//
LOCUS       DACDKL010000018        27096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDKL010000018 DACDKL010000000
VERSION     DACDKL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.018"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(93..287)
                     /gene="ssrS"
                     /locus_tag="IV329_000625"
     ncRNA           complement(93..287)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV329_000625"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(359..1645)
                     /locus_tag="IV329_000626"
     CDS             complement(359..1645)
                     /locus_tag="IV329_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8939832.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1783..2454
                     /locus_tag="IV329_000627"
     CDS             1783..2454
                     /locus_tag="IV329_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8939833.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2420..2908
                     /locus_tag="IV329_000628"
     CDS             2420..2908
                     /locus_tag="IV329_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8939834.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2921..3868
                     /gene="prmA"
                     /locus_tag="IV329_000629"
     CDS             2921..3868
                     /gene="prmA"
                     /locus_tag="IV329_000629"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8939835.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3869..4633
                     /locus_tag="IV329_000630"
     CDS             3869..4633
                     /locus_tag="IV329_000630"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8939836.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4866..7079
                     /locus_tag="IV329_000631"
     CDS             4866..7079
                     /locus_tag="IV329_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8939837.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7090..7536
                     /locus_tag="IV329_000632"
     CDS             7090..7536
                     /locus_tag="IV329_000632"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8939838.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7707..8966
                     /locus_tag="IV329_000633"
     CDS             7707..8966
                     /locus_tag="IV329_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8939839.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9055..10002)
                     /locus_tag="IV329_000634"
     CDS             complement(9055..10002)
                     /locus_tag="IV329_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8939840.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10396..11688
                     /locus_tag="IV329_000635"
     CDS             10396..11688
                     /locus_tag="IV329_000635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8939841.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11703..13472
                     /gene="aspS"
                     /locus_tag="IV329_000636"
     CDS             11703..13472
                     /gene="aspS"
                     /locus_tag="IV329_000636"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8939842.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13581..14456
                     /locus_tag="IV329_000637"
     CDS             13581..14456
                     /locus_tag="IV329_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8939843.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            14722..16605
                     /locus_tag="IV329_000638"
     CDS             14722..16605
                     /locus_tag="IV329_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8939844.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(16680..16964)
                     /locus_tag="IV329_000639"
     CDS             complement(16680..16964)
                     /locus_tag="IV329_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8939845.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(16954..17688)
                     /locus_tag="IV329_000640"
     CDS             complement(16954..17688)
                     /locus_tag="IV329_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8939846.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            17817..18440
                     /locus_tag="IV329_000641"
     CDS             17817..18440
                     /locus_tag="IV329_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8939847.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            18632..19753
                     /locus_tag="IV329_000642"
     CDS             18632..19753
                     /locus_tag="IV329_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8939848.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            19760..22888
                     /locus_tag="IV329_000643"
     CDS             19760..22888
                     /locus_tag="IV329_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8939849.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            22907..26101
                     /locus_tag="IV329_000644"
     CDS             22907..26101
                     /locus_tag="IV329_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8939850.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(26247..26954)
                     /locus_tag="IV329_000645"
     CDS             complement(26247..26954)
                     /locus_tag="IV329_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8939851.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9045 a         4835 c         5808 g         7408 t
ORIGIN      
        1 taaaatccta atcatgaaca aatcctccaa aataagcaac aaatagaaaa agcaatttgc
       61 caagagcaaa ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc gttgtttgtc
      121 ctgtagtatt gatcccgcga attcaagcag gtgggtccca tggtcgtagc ttcgaactac
      181 caaatgataa ggcatgttac cagcagtgac acaagacggg tcccaagata tcaataaaaa
      241 tgttcggtca acacacaaag aggacaattc gaacatcaca gatttcccac ctctatagta
      301 gcatatgaat ttaaaaagag caatgagaga gctcgcagaa acagaaccgt tttcttgtct
      361 agttttttgg aggatgcttt ttccactctt ttatacggta atactgttga cccagagcct
      421 gttcatattt ccctgtatct tttggctgat aatactgagc attcttcagt ttatctggta
      481 aatattgctg attgacccaa gcctgatcaa aatgatgagg atactgataa cccacgccac
      541 ggttgagtga tttcgcacct ttgtaatggc tatctctcaa atgatcagga acatctccag
      601 ctttgccctc tcgaatatca gcaagtgctg catctaatgc catgtaggca gaattggatt
      661 ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc tcaggcaatc
      721 ccaatttttc agcagctaat acagcgttca ctgtacgagc cgcagcagca ggattaccta
      781 aacctatatc ttcataaccg ataaccatca gacgccggca aatacttgcc agatcacctg
      841 cctcaactaa acgggcgaga taatgaagtg cagcatccac atcacttcca cgaatcgatt
      901 tttgaaaagc cgaaataacg tcataatgag catctccatt tttgtcatgg gtaagagctt
      961 ttctttggac acattcttca atgatcgaca gtgtaagatg aatccttcct tctttatctg
     1021 aaggagtcga tagtgtagcc agttccagcc cattcaatgc actccgcaaa tcgccgttcg
     1081 ttgcacgcga aaggtgaaga agagcatctt cgtctaactg gattgcttgt tgtccaaggc
     1141 cgcgttcttt atcccttaaa gcatgttcga ctgccaactg gatgtcttgt tctgttaatg
     1201 gtttgacttc aaagatctgt gtacggctgc ggatcgctgg attgatggta atataaggat
     1261 tctcagtagt cgctccgatc aagatgattc ggccactttc taaatgagga agcagaaaat
     1321 cctgttttgt tttatctaat cggtgtactt catccaataa gaggatcact gtaccactca
     1381 tttttgcttc ttccgctaca acttgtagat ccttttttga atctgtagcg gcgtttagca
     1441 tgcgaaatgc ataattagta gagccggcga tcgcacttgc gatactcgtt tttccagtac
     1501 caggaggtcc atatagaatc atggaagaga gcatgcgagc atcgaccatg cggcgtatga
     1561 tttttccttc cccgactaga tgctgctgac caacgacttc atcgatcgtt cgaggtctca
     1621 tgcgataagc caatggctgt tgcataggat tttcctcctt cttgttttta cgtcccttaa
     1681 ttatatcata tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt gttttcatag
     1741 cttatccaac taatattgtg tatgataaag taggtgagaa aaatgacgga taaacaaaag
     1801 atagatgaat ttttttggaa taaaacgacg ccagaagtgg cacgggattt attggggatg
     1861 tatttggagc atgacacacc agaaggaaga ttagctggat atattgtaga cgcggaagca
     1921 tacctaggtc cagaagacga ggccgcccat agctatggtt taagaagaac accgcgtgtg
     1981 cgggcaatgt atgaaaaacc aggtacgatc tacctttaca cgatgcatac tcatcggatt
     2041 ttgaacatca ttactcagcc agaagggatt cctcaaggag taatgatacg tgcaatcgaa
     2101 cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg tccagatatc
     2161 agcaacggtc cgggaaaatt agtggaagca ttagcaattc ctcaagaact ttatggtcag
     2221 tctatagcag atagttcgtt acgcttagtt tttgagaaaa aaaagacacc aaaaaagatc
     2281 aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa gccgttgcgt
     2341 tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat cgagaaagat
     2401 tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta acatatttag atgaaataat
     2461 tgaaaaacaa gtgacagact atgatgtagc tttagattgg gatacaaaaa atcatacgat
     2521 cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg atgatgcaga
     2581 agggatcgtt tcgcaagaac caattatcga atttgaagac ggcgtacttc tttttaatcc
     2641 tcagaaatct gtttttgatg aacgcaatta tctagcagta atcccttatg aaggaaaaaa
     2701 agggttggca aaatcagtag ctgattcttt agtggaatat ctaaatgaag tattagctca
     2761 agggcaaagc gatttgcttg attttttgga tgaagacgat gacgaagctg tattcgagct
     2821 tcactgggat aatcaacaac tagcagaatt agtagaagta aaacagcaaa atgaagcaga
     2881 tacttattta ccgtacccaa gttattaagg agtgacagat atgaaatgga atgaagtaaa
     2941 agtagaaact gaaagtgaag cagtcgaagc agttgctaat attttgatgg aggcaggggc
     3001 gagtggtgtc gcgattgaag attctttaga tgttgaacat tttcagtcgg atcaatatgg
     3061 tgagatttta gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta
     3121 ttttccggaa acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca
     3181 attacctgag tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga
     3241 aagcaactgg gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct
     3301 gacggttgcg ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac
     3361 actagatcct ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac
     3421 agcactagaa acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg
     3481 cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga
     3541 cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt
     3601 ccatgtatca gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc
     3661 gaacatttta gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca
     3721 aacaggcacg ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc
     3781 catgttagaa caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat
     3841 tattttgaaa aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga
     3901 atcaaaagac tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag
     3961 gatggaaccc gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga
     4021 aatcacggag attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca
     4081 aaaggaactg ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga
     4141 ttggatcgtt cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc
     4201 agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa
     4261 gattgctcaa gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt
     4321 ggaaacgaaa caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta
     4381 tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa
     4441 accagatagt cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt
     4501 cgattatttt gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac
     4561 agaaacagca cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc
     4621 ttctttcagc taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata
     4681 gtcaacaatt tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa
     4741 aaagctttta aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac
     4801 aattatcaaa cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga
     4861 ttcctgtgac aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat
     4921 acatgggtcc cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac
     4981 atgacggaca gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag
     5041 gtatattagc agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg
     5101 ttgttgaaga tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg
     5161 ctatgttagt ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc
     5221 agctagcaga aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca
     5281 tggtcaaatt agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata
     5341 agcaaagaag gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag
     5401 ggatcagccg gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac
     5461 agtattaccg tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa
     5521 gtggtactgt tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct
     5581 atggacggcc aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt
     5641 tcaacgaaat ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt
     5701 atgctgtatt aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt
     5761 atattgccat gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg
     5821 caggaaatcc ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg
     5881 gggttgctgc acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta
     5941 tgacgaaaca attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg
     6001 cttctgaatt tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca
     6061 caccaaaagg agacgttact gagttaccaa aaggatccgg accattggac tttgcataca
     6121 gtatccatac agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc
     6181 agcttgatta caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat
     6241 ttggcccaag tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca
     6301 aacgtttctt caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg
     6361 tcaaatgtat tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc
     6421 aagaagcact cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt
     6481 atggagaagt tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac
     6541 aaaaaatcga gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac
     6601 ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac
     6661 taattcgtat cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca
     6721 ctaaaggcag agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac
     6781 caaatgtagc agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag
     6841 agtatgatgc agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac
     6901 ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag
     6961 ataaaatggc gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta
     7021 tcgtggataa aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg
     7081 aggacagata tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag
     7141 caagaaattg gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat
     7201 acgcaaaaag atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat
     7261 gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct
     7321 caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca
     7381 cgaccagaaa cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc
     7441 atcacagtcg ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga
     7501 cctgtaacga tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa
     7561 aactatgaaa cacgtcgctt aagttagact gataaactct aacttaagtg gcttttttgt
     7621 ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa attatacaag
     7681 ttatttttta taggaggttg ctgaagatga aagaagtacc agtagtagat atcaaaatca
     7741 cagatttaaa aaaattgtat caaaaaggat acaacatgtt ggaggagtta cgccatgaag
     7801 cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg atctacggat
     7861 catcagcagc aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt gctatgccaa
     7921 aattagtatt gaagacactt tttggtcaag gtggagtaca gactttggat ggagctgctc
     7981 atcaccatcg gaaaaatatt tttatggatc taatgacacc ggaaagaatg gaagattatc
     8041 atcgtatctt agataagaat ctgacacaag cacttgaagc acagcatggt caatttgaac
     8101 tatttgattt aagtaagatg gttttcttta catctatttg cgagtgggca ggtatcaatt
     8161 tatcagcgat ttctaaagat gaggtcgaaa aattagcaga atatcaaatc tctatgatca
     8221 gcggaacgtt tacttctccg attgatcaca taaaaggtgt cgagaatcgc aaaaaatcag
     8281 aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct ggaaaagaaa
     8341 atgtggcatt atatgctttt gccaatgcca cagatttaga tggtcagctt ttacctctgg
     8401 aagtggcagc tgttgaatta ttgaacatca tccgtccaac ggttgctcta actgtgtggg
     8461 cggcattgat gggacatgca cttttcagta gaccagattt gtatcagcaa ttaaaaaatg
     8521 acttttcgac gttacaagat ccgtttattc aagaaatgag acgctattat cctttcttcc
     8581 caatgctacc tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt ataccagaag
     8641 gcagttgggt tattttagat ttatatggaa cagaccatga tgaacgaaca gttgaagcgc
     8701 cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc tataaagaag
     8761 aatatgagat gattgcacaa ggcgggggaa attttcgaca aatgcatcgc tgtgcaggag
     8821 aatggatcac tcttcatagt ctacgtgtgt tttccgatca gctagtaaat aagtttgagt
     8881 tctctgtacc tgaacaagat tggacgattc cattcaatca gtttccgact tatccgaata
     8941 gccgagcgct actttacaag aattagatta gcataaactt atcgaaaaaa aggaagatca
     9001 aacagtgctg atcttccttt tttcatatta ctataaattg ataaggtcaa ttgatcaaaa
     9061 tccacgaaat gcttcatcaa tggtttgata atcttcattg gataaatgca aatctaaagc
     9121 ttggacattg tcagatactt gctctggacg cttagctcca ggtataacta cagacactcg
     9181 tggattcttc atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt atcttttagc
     9241 aatggggcga agtacatcga cagcggttaa aatttctctg aaacgttctc cttggaaatc
     9301 tggatttctt tttcgaggat ctccttcacc aaattgtttt tcctcaccaa gttcatattt
     9361 tcctgtcagc aaaccggaag caagaggaaa atagggaaca aaactgattt tgtttttttc
     9421 aagataaggg aataattctt tttcagcctg ccgatggatc aaactgtatt tatcttctac
     9481 tacatcgaca tatccatctg cattcgcttc tttcaattgt tctaacgtga agtttgacac
     9541 accgactgct cggatttttc ctgcttcttt taattcgtgc aatgtagcaa ctgattcatt
     9601 ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat cagtttgcag
     9661 acgttttagt gcatcttcca ccgattgttt gagaaactca ggagaattat caaatgttcg
     9721 ccctttattt ggtacatgag cagcttttgt agcgatgacg acacgtgagc gatcatattc
     9781 tttttccttc agtacctcgc caatcagttc ttcggaacgg ccgtttccgt aagcataagc
     9841 ggtatcgatc aaattgatcc cgctattcaa agctgtacga actacttgtt ttcctgtctc
     9901 atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca tttgctccta atccaagctg
     9961 ttctgcgtag acttgtgaat gtccgattct tatttctgcc atagctgatt cctccttttt
    10021 gtctagtcta aaacaaaata ccaaaaagct gaactaaaac gtttttgtga ggggaaatag
    10081 tcagatatct cttgactttg agactggttt ttagtaggtt ataagtaata tcgaatgtca
    10141 atgaaagagc gagtaagttt ttgaaacttt acaagagagg atcatcgagg ctgaaaatga
    10201 tctaaagttt cattagccta aagacactct ggagatggac gagtgaaaag ccgtcccgcg
    10261 tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa attaggtggt accgtgtatg
    10321 aatttcattc acccttgttc atgatgaaca agggtgattt ttttatttac atagctaaga
    10381 aagggagaga aaactatgag tttccaaaga ccaaaaggaa caaatgatat tttgccaggc
    10441 gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt tattattcag agattatcag
    10501 tataatgaaa tgcgtacacc gatttttgaa cactatgaag tgatatcccg aagtgtaggg
    10561 gatacaacgg atatcgtttc aaaagagatg tatgattttt atgacaaagg ggaacgccat
    10621 gtgacgcttc gaccagaagg tacagcacca atcgttcgtt cttttgttga gcataaattg
    10681 ttcggcccag agtatgcgaa accgttcaaa actttttata tgggaccaat gtttcgctac
    10741 gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga agcatttgga
    10801 tcagaaaacc ctgcaactga tgtggagagc atggtgatgg cacttgattt cttcaaacag
    10861 ttggggatca cgcaaatccg gctagtcatc aattcattag gtgataaaga aacgcgtcaa
    10921 gcctatcgtc aagcattgat cgactatctt ttaccttttg aatcagagct gagtgaagac
    10981 tctaaacgcc gccttcatga aaatccatta cgcgtattgg acagtaaaga caaacgagat
    11041 caaaaatttg tggcagaagc tccatctatc ttagattatc taagcgagcc tgctcaaaaa
    11101 cattttgata ccgttgtttc catgctggat gcattggctg ttccttatga aatcgacagt
    11161 aatatggttc gcggactaga ttattacaca catacgattt ttgaaatcat gagtgacgca
    11221 ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac gttatgacaa tctagtggaa
    11281 gaactaggtg gtccttcaac acctggcttc ggatttgcaa tgggtatcga gcgattactc
    11341 ttgactatgg aggcagagga agtagttatc cctgcattta atgagttgga tgcttacgtg
    11401 gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag tggtccaagc gattcgaaac
    11461 tttggttttt cagctgaccg cgacttcatg aaccgtaaag caaaagcgca atttaagaca
    11521 gcagataaag cgaatgccaa attagtattg gtgatcggtg aagatgagct tgctagtcaa
    11581 acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt agaagagata
    11641 tatgaacatt ttgatgaagt ctatgatgaa atgactttat tcgagtagga ggaaaaaaat
    11701 aaatggcaca aagaacgatc tattgcggat tagtttccga acaacaatta gaacaaacag
    11761 ttacattaaa aggatgggta caaaaaagac gagaccttgg gggagtaatc tttatcgacc
    11821 tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac aaagaagcat
    11881 gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga agtgactggg gaagttagaa
    11941 gacgcggaga aaatgcagta aatcctaaaa tggccactgg tgaattcgaa gtgattgcaa
    12001 gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt tcaaattgaa gatgatcaag
    12061 caatcagtga cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt ccaaaaatga
    12121 ctaaaaacat caaattaaga aatgcgacaa caaaagcgat tcgtcattat ttagatgata
    12181 atgactttct cgatatcgaa acaccttacc tagggaaatc tacaccagaa ggcgcaagag
    12241 actacctagt tccttcacgt gtccatgctg gacatttcta tgcgttgccg caatctcctc
    12301 aattgtttaa acaattgctg atgaatgctg gattcgaccg ttattatcaa atcgttcgtt
    12361 gtttccgcga tgaagattta cgtggagacc gtcagcctga atttacgcaa gtagatattg
    12421 aaacaacgtt cttaacagca gaagaaatcc aaacttacac agagggattg attgcgaagg
    12481 taatgaaaga agttcgcggt atcgaagtca cattgccatt tcctcgtatg acctatgatg
    12541 aagcaatggc tcgctacggc agcgacaaac cggatacacg ttttgatatg gaattgatcg
    12601 atctaagcga tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct cttgaaaacg
    12661 gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga tcgctactca cgaaaagaca
    12721 tggatcagtt aggtcaatat gtaggacaat tcggagctaa ggggcttgca tggctgaaag
    12781 tagaagaaga cggattgaaa ggtccaattg ctaaattcat gggagaagca acagaagcaa
    12841 tcattaaagc aacagatgca aaaccaggtg acttgctgat gtttggcgca gataaaagtg
    12901 aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa ttaggactaa
    12961 ttgatgaatc gaaattcaat ttcttatggg taactgattg gccacaattt gaattttcag
    13021 aagaagaagg tcgatatgtc tctgctcacc atccattcac gatgccaaaa gaagaagata
    13081 tcccgttatt ggctgaagac cctgcaaaag tctatgcaga agcttatgat atcgtcttga
    13141 acggttatga attaggcgga ggttctatcc gtattcatac tcgtgagctt caagaaaaag
    13201 tactagaaac tctaggcttt acaaaagaac aaatggaaga acaatttggt ttcttgttaa
    13261 cagctttaga ttacggattc cctccacatg gcggtattgc attaggattg gaccgttttg
    13321 caatgttatt agccggagaa gataatattc gtgaagtcat tgcctttcct aaaaacggaa
    13381 aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa ttgttcgaat
    13441 tgaatattga tgtaacgaat atagaagaat agccgataaa aaggtaaaaa ggatttgtcg
    13501 aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca ctttataatg aagccataac
    13561 atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat catgattaca
    13621 cgaccaagct ttcggtatct atcgtttatg cgttgatcgc atcagtcgca ttgaatttct
    13681 tttatcagcc aggacatatt tattcaagtg gtattactgg tctagctcag attttgacga
    13741 ctttgtcaaa atcactgata ggattcgaag taccggtatc aacaactttg tatttactga
    13801 atataccttt gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca attttcactt
    13861 ttatcacagt cactttgacc tccctttgta tccatatttt accggaaaca gtcctatcta
    13921 ccgatccaat catctgtgcg ttgtttgggg gggctgtgat gggactaggt attgggctag
    13981 cgttgagaaa cggcctatca tcgggcggtc tagatatcgt gagtatcacg attcgtaaaa
    14041 aaacaggaaa gtcgattggt tctatttcga tctacttcaa tgcattgatc gtttttgttg
    14101 ccggaattct atttggctgg caatatatgt tttacagtac gttgtctatt ttcgtcagtg
    14161 gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg atcgtaacga
    14221 aacatccaga aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg atcacgatca
    14281 tcaatgaagc agaaggtgct tataagcatg ataagcagac agtattgatg acgatcgtta
    14341 cacgatacga actgcctcta cttagagaag caatgaaagc ttctgatcca caagcttttg
    14401 taagtattgc agaaaacgtc cagatacttg gtcgtttcta tgaggatggc atttagatcg
    14461 agtttgacaa aaattcatga aatactgctg tagacagacg ttcgtttgtc tgcagttttt
    14521 ttctaccata gtagaatcag caaagaaaaa ctgctttttt atttgaaatt ttcttaaagt
    14581 tgaaaaaaag catgtttatt tttttaaatt tatgggatga tagaaaaaaa tagtagagaa
    14641 aggacacgat tctacttgct attttcactt aatacccgta tattacgaat gaaaaaagtt
    14701 tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt taaaaaacag ccgctacatc
    14761 actgggtttg atgggctccg ttcattagct gtggtgggtg ttatcttcta tcaccttctg
    14821 ccaactagta tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt atctggttat
    14881 ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa tatttggcaa
    14941 ttttatgtca gaagaatgaa gagactctat ccggcacttg tttttatgct tgtgacagct
    15001 tcggcttata tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg agttgtggtc
    15061 agcagcttac tgtatgtcaa caactggtgg caaatcaata acggtttatc ttatttcgat
    15121 cgatttgcta atgagtcacc gtttacgcat atctggtctt tagcagtaga aggtcaaaac
    15181 tatttggttt ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa aagaaaatgg
    15241 atcttttata ctgtgttagg tacatccttc ctctccgcca tcttgatgat gatcttgtat
    15301 acaccaggag gcgatccaac ccgtgtttat tacggtaccg acactcgtct gttttctata
    15361 tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa aaacatcccg
    15421 aaacaagcga aaaatattct gaatatcgct ggaggaatct cgcttttggc actgattctt
    15481 tgcttcttct ttttagatga tcattacaat ttcatctatt acggaggaat gctcctagtg
    15541 agtctgttat gcgtagtgct agttgcagtg acagcacacc ccggagcaag tttgaatcga
    15601 tggctgacaa atccagtatt tacttggatt ggcaaacgaa gctatggcat ttatctgtat
    15661 cagtttccag ttatgatttt ttatgaagca aaggtgactg atattgccga tcatgttttg
    15721 ttgcatactt tagtggaaat cacattgatt ctaatcatca gtgaactttc ttatcgatat
    15781 gtggagcgtc cgctagcacg ttttgattat ggtcgtacat tccaagtgat gaaaggatgg
    15841 ttcagcaaac cgatcatcag taagagcaaa ccatgggttg ttccaggcac attacttgta
    15901 gcaattgcac tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc cgatcaaaaa
    15961 caactgcagg caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag aaacaattca
    16021 gaaaacacca ctagttcggt tgaccaagcg atccttgata agtacgagtt gtccaaggca
    16081 caagggaata aggcacaaaa attagaacta acggcatttg gtgattcagt tatgttggat
    16141 gctgcaacgg atctacaaga actttatcca aaagtagtgg tagatggaga tgtcggacga
    16201 caattatatg ccagtgtacc gtacattgaa aaattaaagg aacaagattt gttgaaagac
    16261 actgttctga ttggactagg aacgaatggc tcattctcgg aagctcagtt tgatagtgtg
    16321 atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc gacgaagcgc
    16381 tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga aaaaatacga caatatgacg
    16441 ctgattgatt ggtacagcta cagtaatgac catagtgact ggttttatga tgatcaagtc
    16501 catccaaatc ctgaagggat gacacattac attcacttag tcagcgaagc cttgctagga
    16561 ggaagttcaa gtacgaacag cagtgagaca aacgtgacag aatgatagct acaaaaaact
    16621 tcaggcaaaa acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt
    16681 catatagatt caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt
    16741 cgggtgagtt ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact
    16801 tctgcatgga tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta
    16861 ccattgttat gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc
    16921 tgatctttac agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat
    16981 ctctgcagtg tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc
    17041 gtctaatttg ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt
    17101 tgggtaaaca aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat
    17161 ttctaagaac cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt
    17221 acttgaaata tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag
    17281 gtgtttatta gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac
    17341 gactaagtca caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg
    17401 gacagtcatt tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag
    17461 acgaggttga agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc
    17521 aactgcgcca tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa
    17581 gtgagcaagc agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt
    17641 gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca
    17701 aaatgaaaaa aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt
    17761 gtcaaacctc tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt
    17821 tttatcgatt tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc
    17881 gctatgagaa taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc
    17941 gtacttggtg ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta
    18001 tggcaaaaga agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg
    18061 cttttccagt taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt
    18121 tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac
    18181 ttaaaggcgg aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat
    18241 atcaaggtcc gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg
    18301 gcgaaccaat cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg
    18361 aacgacgaac acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat
    18421 atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg
    18481 tcctgttcag ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt
    18541 tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa
    18601 taattagaag gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca
    18661 tatcggaaaa aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc
    18721 tatactagat atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata
    18781 tgataggagt gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg
    18841 gaatttaaag gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag
    18901 actttcagcg ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc
    18961 agaagcattt gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga
    19021 accaattgca gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt
    19081 agccaaggtg atagataaaa tgcaggagct atttgattca gaaaaaaaac aagtattgat
    19141 tagccatttt tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa tcgaagtagg
    19201 cgggttggat tcgatccctg gtgaactact tacaccgttt gattatgtag cactaggaca
    19261 tttgcataat caagctgctt tgagacagga taatgctcgc tatagcggtt ccccattgaa
    19321 attctcttta tctgagatca accaaaaaaa aggcgtatgg atcgttgacc tgacaacagg
    19381 cctggagttg aattttaggg agatcgaacc cctgcatgat attgaagaaa taacagcgag
    19441 tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc aatcgggaga attacatcta
    19501 tatctatctc actgatagaa cgatcatccc gaatatgatg aaccagcttc ggcaaatata
    19561 tcctcgtatt ttggggattg aacgggcaaa cggtcgagca gaacaaacaa agcgcaaacg
    19621 aaaagtacta gaaatgaaag atcctcaaaa attagcagaa gcatttttta gtgaaatgac
    19681 ggaagagcag ctaactgcaa accaagaaaa atggctcgga gaaacgttgc aagaaatcat
    19741 ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt ttggtccttt
    19801 tatcaatgag accgtagatt tttcacgttt gacagaagca ccgctttttt tgatcagcgg
    19861 gaagacagga gctggcaaaa cgacgatttt tgatgggatc accttcgcac tttttggtga
    19921 aacttccgga agacttcgct ctggaaaaga gatgcgttct ttgtttgcta caccagacga
    19981 agaaacaagc gtgacttttt cttttgaaca tcagcagatg agctatgaaa tcgaacgaaa
    20041 accagaacaa gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg cagccaaagt
    20101 aacgttgacc atttttgatg ggaaaggaaa agaaataaaa caaatcacaa aacgtacaga
    20161 agcagatcag ttgatcagag aactgatgaa tctcgatgcg aaacagtttt ctcaaatcgt
    20221 tttactaccc caaggagaat tccgtaattt tttggtttca tcaagcagtg ataaagagac
    20281 tgttttacgt catttattcg gtactcaatt tttccaacag ttcaatgagc gattaaaaga
    20341 aaaagcaaaa caacagcaac aaaaattgga tcatttagag caagagctgc atcttctgca
    20401 aaaaaggttt gttttagtca aagaagagga accagcagca gggtttgaag ccgttttagc
    20461 ccaatggaca aaccatcagg caatactcga aaaacaaatc acggaagaaa aagaagctct
    20521 aacggaactt caggaaaaac aaaagcaact agaaaattcc tactatcgtt ttgaaagctt
    20581 gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag caaaaggaat tagctgctca
    20641 aaaagacgaa atagaggaaa agaaacgatg gattgggtat tatgaattta cgcaacgatt
    20701 agtcgaacca atccagcgcc tacaagaaat aaaagttgaa aaaatagaac tgtcacaaaa
    20761 aagtagtgag caaacaaaga aactagcgat gactacgcag aattatgaag agtggcaaaa
    20821 aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc aaaaactgca
    20881 aaacaaacag atgatggtgc caattgtcga atccttacgt agtaaaaaac aagacgcaga
    20941 acaattaaca atttcattgg ataaagaaga attgaatgcc gagaagctgt atcaaaatct
    21001 cgctatctat caggaaagaa ctcgaattct tcaagaaaat ttactggatc aggaaaaact
    21061 gcaagaagat cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc agcaagaaat
    21121 agaaaaacaa aaagcaaaag acactgggtc gctcgcacag ataagagaag aactcaaaaa
    21181 agcaactgaa gaaataacaa aaaatagtca gctattgaaa gaacagacag aacaagcaga
    21241 acagctgaaa agccaatggg caaagcagca aattgctcgt cttcagcttt ttttgcgtcc
    21301 aggggagcca tgtcttgttt gtggatcaac ggaacacccc atacaacaat taaaacacga
    21361 acagccaaat aaggaagata tcttaaaagt agaacaacaa ctggcagaag ctgagtctgc
    21421 tgtagaaaga actcagcaaa tcattgccaa aagtgaagca catgaaaata atcttcaaca
    21481 acaattatca gatttagaac aaaaaatcat ggcaacgaat atacaattgg ctgaacaaga
    21541 agaacacttg caacaggctt tgaaaaaggc atttcctgag ataacagaac aaaaaacaaa
    21601 aacaactctt gtagtaatcg aacaatcttt agatagacga aaaagcaaga tggccaatga
    21661 aagaatagag ctggaagaaa taagacaaca agcagaaaag ctcgaacaac aattagctgt
    21721 ctacaaacag cagcagacag aaaaaaataa tcagctccag caactgcaag gagaaatcaa
    21781 aagtttagaa caacaattgc aagatacgga tagtaactgt ttagaagagg agatacgacg
    21841 acttgaaaaa cagttagaag aagactcgaa tcaactagct gttgagcaga aagcaggagt
    21901 gcaattgttg caagatcttc atacgcttcg gacacagctt gatcttcaac atgaacaaat
    21961 agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa agttagaaag
    22021 ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc aagcacaata
    22081 taaagaaaac caacgtacag ttacttctta tcatgaagag caattagtga cagctgaccg
    22141 gctgcgtcaa ttagaaaata tccagctaac tgagacattc ccagatacag aagagatcag
    22201 ccaacagtta gaagaaataa gacgtatgat cgaaataaag caagaaaaga tatacggttt
    22261 acaagaacaa aaaattagta atcaaaaaat ctacgatgaa tgtctttcga tttatcacac
    22321 aagccagacg aaattagatg agcttacgca attacagcag ctttcgcaaa cattcaacgg
    22381 agaaaatcct aagaagacta gtttggaacg gtatgtgctt caggtttatc ttcaagaagt
    22441 gttacaagta gcaaatgatc atctccaacg tttaacgaaa aatcgttatc aatttgagct
    22501 agcggatacc attggcagtt atcgagggaa aaccggatta gaaatcaata tctatgatga
    22561 tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt ttattgcagc
    22621 actttcttta gcattagctc ttgccgatgt gatccagaca caagctggtg gtgtagcaat
    22681 cgatgcattg tttattgatg aaggatttgg gtcattagat gaagaagctt tggaaatggc
    22741 aatggaagct ttagaaacga tcgaaagtga aggaagaatg atcggcatca ttagtcatgt
    22801 aagagaattg aaagaacgag tattacaaca aataagaatt gatacagaag gtagcggaca
    22861 aagcaaaata cgctatcaac ttggataaaa agggagattg gtaactatga aatggagtat
    22921 tccagaaaaa gtcattgcac gtggacgtca atacttgcaa gaaggccgag tgttatcggt
    22981 tgtccctgac caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg aattgtatat
    23041 ggttgatttg gatggtacag caaaagagat agattactgc caatgtccct attgggagga
    23101 acataaatat tgtaagcata cagttgctgt ggaactttat ttacggtcga aaaatctatc
    23161 tcgtatcatg aaaaaagacc agccaatgct agaaccggta aaagcaacaa gtgagggaga
    23221 aatcattgca aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag cggtgcaacc
    23281 tttgattatt gaataccaag tagaaacgat tgagactaat cagtatcacc cagagctttc
    23341 tattttggca ctttacttgc gtatcggttc attggcatca ccgaaaaaaa catatatcgt
    23401 aaaaaacatc tatgattttt tacaagctta cacagaaaaa gaaacttaca ctgttaataa
    23461 acagtaccag tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa aaatactcac
    23521 gcatcttgca ggaagtgctc agacgcatca attattaggt acgaacggct tacaagtcaa
    23581 aggaaaacta gataaaagat atctgcttct cccagtagaa caagcccgct tcctgttaga
    23641 gcagatgaat cagactacac gtttgcatct gcagataaat gaacaaaacg atcaaggaat
    23701 acaattttct gatcaaaaca aacctttatc attcaaagtg gaaaaatttg gagaagatta
    23761 tttgctgcaa gcattgaatg attttgattt tttctttatg cattatcagt ggggagttat
    23821 ccaagggaca ttttattctc tgacgaaata tcaacaaacg gtttatcaaa cttggaaaca
    23881 attgattcgc cgattggaga aaccagaagt agtgttcaag aaaaaagaat taccgtcttt
    23941 gtttaaagag attatccctc tgctgtcaga gatcggcgac gtagctattg cccctgcagt
    24001 agaagaggaa gtagcagata tccccgtcga atttattttc tttttccgaa aagcaaaagg
    24061 aaaaatccaa gctcgagtcg atttcgtcta tggtgaagtc atctattcga ctgacccgaa
    24121 gcatgaggtc tcgacagaac aggcttttca ggtgttacgt gacacagtag aagagaaacg
    24181 agtacttgat ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt atgaacgtcc
    24241 attgccaagc ggggaagaac tatatgcctt tttccgcaca gaactatctg tcttccgtca
    24301 gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg tatttagatg cgcataaaca
    24361 tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc gatattcgat tcgatgtgac
    24421 agggatagag gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa atgatgcttt
    24481 ttatacatta gaaaatggtc aggttctttc ttttgattca gaagaatttc aacaaacaag
    24541 ccaagtgttg caacaattac gggaatcgat acgtacagaa gaaggaacga tccatgtacc
    24601 taaaaatcaa ggattgatca ttcaaaatca attggaaaaa agcaatgcta cttttagtga
    24661 atcgttccaa acgatggtac aagatctcat tcatccagaa agataccagg cacagcttcc
    24721 caaaggattg aatgcaacga tgagagatta tcaaaagcaa ggttttcgat ggttgaagat
    24781 gttaggtcat tatcagtttg gcggtatttt agccgatgaa atgggacttg ggaaaacact
    24841 acagacaatt gcctttttac tttcggaaaa agaagaacgg aaaagcttct ctgctttgat
    24901 cgtagcgcct gcgagcttaa tttacaactg gcaggcagag gtgagaaaat tcgccccttc
    24961 tttaagtatc caagtaatca atgggaataa aaaagaacgt gaagaattac tggctaaaga
    25021 cacagatatc cgagtgactt cttatgccag cttaagacag gatctagcta attatcagtc
    25081 gcaaaaaatt gattatttga ttcttgatga ggcacaaatg gtcaaaaata gcagtacgaa
    25141 aactgcacaa gcattgagag aattagctgt acctcagcgg tttgcactga gtgggacacc
    25201 aatcgagaat aatttagaag aattgtggtc attgtttgca acgattatgc caggattttt
    25261 cccgactaaa acaaaattca gggaacttgc cgcagaagaa attgcgcaaa tgattcgacc
    25321 gtttatttta cggcgagata aacaaactgt gttgaaagac ttgccggaaa aaacagagat
    25381 gaatctatat tctgcactta cagaagaaca aaaaactgtt tatttagctt acttgcgtca
    25441 aatgcgtgaa gaaatcacat caatggattc tgaagcattc aagaaaaacc ggatcgggat
    25501 acttgctggt ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt ttattgaaga
    25561 ttaccaaggc ggttccggca agctcgaaca agtaaaagat ctactggtag ctgccaaaga
    25621 gaacaaacga agagttttgc tgttttctca atttacaggg atgctgacta tcttgcaaga
    25681 agagttagcc gagctaggga tctcgacatt ttatttaaga ggcagtacga aaccacaaga
    25741 tcggttaagt atggtagacg catttaatgc tggcgaaaaa gatgtttttc tgatttcctt
    25801 aaaggctggg ggaactggat tgaatctcac gggagcagat acggtcatct tgtatgacct
    25861 ttggtggaat cctgccattg aagaacaagc tgctggacgt gcacatcgga tcggacaaaa
    25921 aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg attgaggaac gcatggattc
    25981 actccagcaa gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg aagaacagtt
    26041 gacgaaactg actgaagaag acatccgcat gattttaagt gtaggagaaa tcgaggagta
    26101 agagactcga aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa gcgattgttt
    26161 atttgattta aaaaatagca aggaggctgg gattccattt tatcccagct tccttgctat
    26221 cattttttta tttctatttt taaaggttat acaaagattg tcttcattgt ctctaacagc
    26281 tgatcaaaag aagaaatctc gatatcgcaa attggagtct gttcaatttt gcgttctcga
    26341 tggttgaacc aaagcgcttg ccagttagca ctttttgcac ctagaacatc attgtcgtaa
    26401 ttatcgccaa cgtataaggt ctcttctgga agcatatgaa attctttctc agccagctga
    26461 aaaatctctt tttctggttt ttggaaacct gttgcttgag aaatgagcat attgcgggaa
    26521 ggaatccaat tgttcaattg aagttgcttc agtttttttg tctgatggtc agtcggccca
    26581 tttgtgataa ttcctaatgg gacattcttt tccttcaaga aatcgagggt ttttttgacc
    26641 tcctcatgca agcagatatt atcaagttct tcctcgtaaa ttttttgaaa taagaggcca
    26701 tcttcttccg taatatgagg gtagtctaaa tcttttagtg attggctgat ccgatgtgct
    26761 cgcatatatt ctagtgtcca atctcctgcc attacttttg ggaagttttc atcactatga
    26821 tggcggaacc ggatatatag tggatgcata tctgcatctg aaacaagcgg aacgactctt
    26881 ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac atcaaacaca
    26941 actgatttaa tcaaaatttt acacgccttt cggttttcat tgattttttc acaaatcctt
    27001 gactattgta acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt ttcttactat
    27061 atttctattt ccttgacaga ataaggtttt tatatt
//
LOCUS       DACDKL010000019        26303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDKL010000019 DACDKL010000000
VERSION     DACDKL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.019"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(106..1050)
                     /locus_tag="IV329_000646"
     CDS             complement(106..1050)
                     /locus_tag="IV329_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8939852.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1271..2485
                     /locus_tag="IV329_000647"
     CDS             1271..2485
                     /locus_tag="IV329_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8939853.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2507..4522
                     /locus_tag="IV329_000648"
     CDS             2507..4522
                     /locus_tag="IV329_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8939854.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4605..5978)
                     /locus_tag="IV329_000649"
     CDS             complement(4605..5978)
                     /locus_tag="IV329_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8939855.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6004..6288)
                     /locus_tag="IV329_000650"
     CDS             complement(6004..6288)
                     /locus_tag="IV329_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8939856.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6290..8305)
                     /locus_tag="IV329_000651"
     CDS             complement(6290..8305)
                     /locus_tag="IV329_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8939857.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8484..8789)
                     /locus_tag="IV329_000652"
     CDS             complement(8484..8789)
                     /locus_tag="IV329_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8939858.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8878..9909)
                     /locus_tag="IV329_000653"
     CDS             complement(8878..9909)
                     /locus_tag="IV329_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8939859.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10116..10991
                     /locus_tag="IV329_000654"
     CDS             10116..10991
                     /locus_tag="IV329_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8939860.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11143..11985)
                     /locus_tag="IV329_000655"
     CDS             complement(11143..11985)
                     /locus_tag="IV329_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8939861.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12077..13954)
                     /locus_tag="IV329_000656"
     CDS             complement(12077..13954)
                     /locus_tag="IV329_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8939862.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(13951..15372)
                     /locus_tag="IV329_000657"
     CDS             complement(13951..15372)
                     /locus_tag="IV329_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8939863.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15375..18764)
                     /locus_tag="IV329_000658"
     CDS             complement(15375..18764)
                     /locus_tag="IV329_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8939864.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19021..20412
                     /locus_tag="IV329_000659"
     CDS             19021..20412
                     /locus_tag="IV329_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8939865.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20485..21132)
                     /locus_tag="IV329_000660"
     CDS             complement(20485..21132)
                     /locus_tag="IV329_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8939866.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21140..21826)
                     /locus_tag="IV329_000661"
     CDS             complement(21140..21826)
                     /locus_tag="IV329_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939867.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21832..22905)
                     /locus_tag="IV329_000662"
     CDS             complement(21832..22905)
                     /locus_tag="IV329_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8939868.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23127..23801
                     /locus_tag="IV329_000663"
     CDS             23127..23801
                     /locus_tag="IV329_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8939869.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23806..25146
                     /locus_tag="IV329_000664"
     CDS             23806..25146
                     /locus_tag="IV329_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8939870.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25295..26281)
                     /locus_tag="IV329_000665"
     CDS             complement(25295..26281)
                     /locus_tag="IV329_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8939871.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7652 a         4965 c         4824 g         8862 t
ORIGIN      
        1 tagaactgaa aataaatcca aaaaaattcg ctgcaacgat ataataagac agcaatcgca
       61 cctaaaacaa ttttccttca tatgctttag tgaaagattt tcttcttaat gtttatttct
      121 ccattcattg ggagtacatg ttgaatactt cttgaataat ttgaataaag aagtcgtttg
      181 tttatatccg atacgattag cgatttcttc caacgaaaaa ctcgtttctt gaatcagttc
      241 caacgcaaag tcgaattttt tctttagaac cagttcttga aaggaagcat caaaatacat
      301 tttcaattta ttactaagat agaccggatt gtagccaaaa tgagctgctg tttcttttaa
      361 cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct cattttctgc
      421 gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt cgatcagtaa
      481 tagtgatgta tagagattta acgaacggat cttatgactg tatgtatcaa aataaatcaa
      541 cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa aatgaatgaa
      601 ttgtgtatgt tccccttttt cttttgatgc atttatcaaa aaagaagaaa gtagattatt
      661 tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta ataagatgtt
      721 gattaaaata tcctcttgcc ctgtgtattc gattctttga gtagcttctt tatcaatgat
      781 caccacgtca ctttcttcca tctctatcct ttctccatta ataatttgta aacatgtacc
      841 gctatacata taattgattt caacaaaatc atgtgtatgc tcgggaactt ccgcatatcg
      901 acggtgttta ctgatatgaa tatttccctg ttgaaataga tcccctcggt ggatttttgg
      961 cggtgtctga agatggtaag ctaagtttaa atcattgata ttcattcctt gttcacgctg
     1021 cagagtttcg attgattctt cgttacgcaa aaaaaatgtc taattcagct agttccaatt
     1081 gactccctcc tttttgtaag cggtttcttt cgcttattag ttcacgctgt actgctatta
     1141 tatgcaaagc tatttttggt ttcataaaga ctttctatgg ttgggacatg aacttcctgt
     1201 gattccgcta ttgagagaat aaccacttca tactatattt ttgataagcg cttacaaaaa
     1261 tgaaaggaac atgcttatga ataaaaagat gatgaaattt tctctgctgt cagtctctct
     1321 tattttgact tctgctggtg cagtcacgtc tgcaattccc agtatgtcag ctacttatgc
     1381 cacgcgttct ttatctgctg ttgaattatt aacgactgct ccctctttta tggtcactat
     1441 ttttgttttg attagtagtt ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat
     1501 cggattagtg attgcatcga tttgtggtat cctccccctc ttctcacaaa gttacagtat
     1561 tgtcttagct tcaagagctg gactaggtat tggctttgga ttgatcaatt ctttggcagt
     1621 cagcatgatt gctgcctttt atcacggaga tgaacgagcc tctatgattg gttttcaaag
     1681 tgcatttcaa ggatttggtg cagctttaat gacatttgtt gcaggacagc tagtacagtt
     1741 cggttggcaa tatacctttt tagtctacgc catcagctta cctattcttg tcttgtttgt
     1801 tttgtttgtt cctacacctg aaacaactga acttcaatcg catggcacag ccccaacaag
     1861 acaagtaatc aaaacgaatc ctaaaatgat tagttatggt atttttctct tgctggtcat
     1921 cgtggtatat aatgctgtat cgattaaatt agccacagtc ctgacgactt attctctagg
     1981 aaacgaaact aatgcagcga atatcttaac aatcatgcaa ttggcaagta tggctgccgg
     2041 agtttgtttt ggctggcttc agacaaaagc acatcgctat atgcttacac tttctttact
     2101 actgatggga atcggctttg tattgattgc cactgtgata aacctgtatg tgattgggtt
     2161 tggtgcgatc ctaacaggaa tttctttttc gttattcatt ccttacttat tcaaccaagt
     2221 aagtatccaa gcgcccaagt acgcggagag tttcaatact tcaatcttat tggttggcgc
     2281 taatttaggt actttcattg caccatatgg tttgctcttt ctctcttttg cttctcttgg
     2341 gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat
     2401 tagccttcta gtagtacaca ggacgaatag aaagacagtg ataagagtag atgaagaaat
     2461 aaaagaacaa ttgacaacaa aataaatttg atggaggtcg tgtaaaatga caaatgaaaa
     2521 acataaagat agatggcttt ggtatcctgg agactttgag atccgccatg gactgctaca
     2581 aaatttccaa cgggaagaac gaggttttga ctggccagcg tactggtaca tggatgattg
     2641 ccatcggaac gtcaagttta agagatatta ctttcttgat caaccttcca tgtttaaagt
     2701 gacgatccaa ggagttgggt atgtagaaat taatggacaa aaacatccat gtggaaaatg
     2761 gctgacttgt ccagctggta aagcaaaaat tcgtatcttt gtaggacata catcagggct
     2821 accagcgatg tttatcgagg gagacgaagt aaaatcagat attggctgga cagcaagtaa
     2881 ttttattgaa gaatatccag ctggctggag tccgctttat gtcgatatcg caaaagatcc
     2941 aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc actgaaacgg agattggcgg
     3001 aggaatctta ttcgattttg ggcgagcagt gaatggctgt gtagaactaa tatctctgga
     3061 tgaccatatc gaatctatca ctctatgtta tggagagtcc gatgtagaag ctatggatat
     3121 cgattattgc tactataagc aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc
     3181 ttttcgctat ttgtttatac cagaggtttc tactggaatg gttcaacttc aagtttttca
     3241 tgaattttta tctctttcga aacaagctgc tttttcaagc gattcttctt tactgaatca
     3301 gatctggtca gtatcagaag aagccttcaa gttatgtagt gatttgtttt ttatcgacgg
     3361 tatcaaacga gatcgctgga tttggtctgg agatgcgtat caatcattca tgatcaatca
     3421 atatcttttt tttgacgagg aaatcaataa acgaacgata ttagcattaa gaggacaaaa
     3481 tgaaatcaag cagcatatga atactatcgt tgactattcg atcttgtggt tgattggtat
     3541 cgaaaaccac tatatgatga cgaaagacta tgaattttta cgcatcgtct accctaagat
     3601 gcaatcatta atgagatact tgatggagca aacgaatgaa ttagggttta tttatgggag
     3661 agcaaaagat tggatattta ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc
     3721 agaacaagtc ttattactga aagcctatca ttccatgatc acttgcggag atatcttagc
     3781 tgaaacatat ggagagaacg aaaattatca tactcatctc tatcaagaaa aatatcaact
     3841 attgaaacaa aacctatttg actacttttg ggatgaagaa cttggcgctt ttatcgattg
     3901 ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat attttcgctg ttttattcga
     3961 ttttgctaac aaagaccaac agcaatcgat cttaaagaat gtcttattga atgacactat
     4021 cacacagatt actactccat atttcaaatt ctatgaacaa gatgctttat gcaaactcgg
     4081 acagacgaat atcgtctacc aaacgattct tgattattgg ggagggatgt tatctcatga
     4141 tgcggtaact ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa agtacagtat
     4201 gtatggagat ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc caatctatct
     4261 tttaggacgc tattttatcg gtttgtatcc aacaaagcca ggttatgaag aatttatgat
     4321 acaacctaat ctagattcat ttgaaacact gaattgtacg ctacctatca aagatggaaa
     4381 gatttcgctt gaaaaaacaa aagataagat cgtgattact tctgatagaa caggcggaac
     4441 agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa cagccacttg tcttaacaag
     4501 ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg acatatcaga agactttaaa
     4561 tcttcttctg atattgtcca agcaattttt attttgaagt ttgcttaatt ctgttcttta
     4621 gaaacaatgt ttgttctttt tccggcaaaa agaaagctag ataatacgac aagtaaaaag
     4681 aaagcgatga ttccaactgt caaggctatt tgaccgtatt ctcctagttt cccaagtaca
     4741 agtcctggta ctagataatc agtatcggca aatgtagaag aagcgatttt aagatctcct
     4801 aatactggaa ggataaataa tggcagccaa ctaatcaata agctattgaa agcagaacct
     4861 aataccgccc ctcttctacc gccagtcgag ttgccgaaga caccagaagc agctccgcag
     4921 aagaaatgtc ctactacccc aggaatgata actgtagttt gcaagcctat catagcaaac
     4981 atggatagtg ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc attaggtgca
     5041 tatggaaaaa cgataggaac atctaatgct ggtttagaat cgggtacgag ttttttagca
     5101 atcccttgga atgctggaac gatttctgcc aacaccattc gaaccccttg aaggacgata
     5161 acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa agattacagc gttgtctcca
     5221 ttgcttagtt ctctttcaac aaatccagga ccagctaaca aagcaagtgc gacatatacg
     5281 atcgacatca agagcatgat cgatacagta gaatctcgca aaaagctgaa acttttagga
     5341 atctttatgt cctctgtaga acggtctttg tttccaaaaa actttccgat ccatccacta
     5401 gaagcataac cgatatttcc ggtatgccct aacgcaactt gattgtttcc agtgatgttc
     5461 ttcataaacg gttgacaaag agcaggcgta atagttaata gtgttccttc gaagaccccg
     5521 cctaataaga tcatccacaa atttgcagta aaccccgcgc ttactaaaat gactgctgtt
     5581 agacaagaaa cataaagcat tgcttgacct gtaagaaaaa tgaatttata tcttgtgaat
     5641 ctagcaagta aaatattcac gatcatccct actagcatga tcaaagcagt aggtgtaccg
     5701 tattgttgca aagcaatcgc cactactgct tcattgctag gaactactcc ttgagtatgc
     5761 agagctgttt ggaacatcga agcaaaaggg gttagtgaat tactgattaa accagcaccg
     5821 ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc cttggatcac ttctgaaaat
     5881 ttcttcttct gaagaacgag accaagcagt gcaatcaaag aaacgaggat ggagggctga
     5941 ctgacgattg acacaaacaa attcaacaat ttatccattg atagttcctc ccttgaatca
     6001 tttttattct tttgacaaga tcttcgttac tttttctttt acttcttcgc tgtcaatgat
     6061 cgattgcaaa ggtacgagct tttcatcagg tatagaagtt agcgtatctt tcaaagaatg
     6121 ctcaatgaag aaataatcag ccatatcgct agatacactt ccaacatccg tatgacttac
     6181 ttcaaactcg tccacatctg ctccaagatc tgcaagtgct tgtttgatgt tcatctctac
     6241 cataaaactt gttcctaatc cactttgaca tactgcaaaa attttcatat cattcgtctc
     6301 cttttcttat gatcgctaat atttcttctt ttgttgatgc gtgtaataaa tcttgaagat
     6361 tttgtttatt agaaagtagc tttgttaaat ttgccaaagc ccgaagatgt gcctcattat
     6421 cgatagcagc taaacagatg aacaaactaa tgggatgggc tggattatca agcagatcca
     6481 caggttgttg aagtttcagc aaagacatcc ctacttcgtt tacgccatct tctggacgag
     6541 catgaggtaa tgcgatgtga tcgccaatat ggataaacgc accatactgc tccacgcgct
     6601 cgatcatagc tttgatataa ctttcttcta ttttattttc tgataatagc ggctgtgcgg
     6661 caaatgtgat tgcttcttgc cagttttttg gttgatccga taattggatc atttcttctg
     6721 ttaataattc agatagcata ggacgttgat cctccttctt ttctaaaagt ttgttcattt
     6781 tacgatttaa tacttgatag agcttttctt cgtctacgcc ttcttttaat tcgatataag
     6841 gcagtagcgc cgacaacaat tcttgggcag aaggcattga aaatcctggt atcagccaat
     6901 ctctttgtac ttgattgatc agctgatttt tttccaagtc agtcatgatt ggatgcagaa
     6961 catagagaga ctttttctta tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt
     7021 aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt agtctctgta aaaaagatca
     7081 tcgggaataa ttgctgaagt tcacttttta agatcaatga agagctaatg ccacttggac
     7141 agacgatcac ggctcttatt tttctatttc tttcttctgc gtccgctttt ctgatctctc
     7201 cgccaaatag tatagtgaaa aaaccaatct cttcttcagg gatgctcttc cctattcgtc
     7261 tttctaacgg aagtaatgct tttcttgtca tttcgtagat ggaagcatat tgatgcttaa
     7321 tttgatcgat catcacatta ggtaaataaa aagaatagga aatacgaaaa taggcaggga
     7381 ccaagtgata aaagacattc atcaataatt cacgaaacga agcaaattct acggccatca
     7441 ggaattccat gcgatggatg acttctgaag aacattccag caaaaaatca agtgcaggct
     7501 cctggacatc tccttcaacg atcgatgcta agcaaagaga gaaaaatata atctccgaat
     7561 tttgatgttc ggaatagcca aggccagtca atacttgtcg actaatgtca gtgactgcaa
     7621 gagaagccaa cagttcagca tcttttacca gttccatgga tgtttttcca attcgaatgg
     7681 ttgtcaaact caaataataa gctaattcat ccattcgatt tggtgcgaat cgaagatctg
     7741 atagtgaaat cgttttatct agcaaatctc taactttggc gtaataatca atggaaaggg
     7801 ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc
     7861 gattttttgc ttctcttctt aatattgctt ctgaccctag cagcctaaaa ccagttttcc
     7921 tagtatacga cagctgacaa tcttccactt ctaattcatt tcttatcttt ttgatatctg
     7981 acaggactgt ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg
     8041 ttgcagtcga ataaaccatc aaataaagaa gatctcttcg ctcttgttcg gtatacacag
     8101 cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc gattgctcgg aatttcttta
     8161 tacggatacg ggtatcatcc acatattcgg caaaaggagt taattgtttg atcgcctcgc
     8221 ttgtatttac ttcgatgatc tctgctgctt tttctagact gatttctctt ggtgttttca
     8281 ggagataact aattttgtag ttcacagcgc tgttcacctc ctacttatat tctagtatgg
     8341 tagcgctttt aaatcgaggg gtgtttggta caacttacag ttggattttt tttacgactg
     8401 taccgaacga tttctatcga atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc
     8461 ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt tattttttga ggtcagttct
     8521 tcaaattttt ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc
     8581 tcattaggct ttgtttcctc ttctacagaa tttgaaccag ataaagctgt ttctttattt
     8641 tctgtttcac cagagtttgt gccagctaat gattcttctt tgtcttctgt ttcaccagaa
     8701 ttagttcctg ctaaagccac ttcttcttct gttttagggg ccattgaacc tcttaatcgt
     8761 ttgtgttttt caccgtcata atttgtcatg ttggttcctc ctagtagttc tatacttttt
     8821 catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca
     8881 tatttttcct tctgattctt gaatgctttt gattccttct tccaaaattt tcatttgtaa
     8941 gattgtttct tcatctttga tgactttttc tgcgccatta actaatgtct cgtaaatccc
     9001 ttcgtagaca cggctgtaat caccaacctc ggaaattact ttttcttcat gataactccc
     9061 ttgttcatct atataggtca atacaccata atgttcgggc aaatcgatac caaagtcttc
     9121 atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg tttgtttcac
     9181 gaacatccct ttttttccgt aaacgacgaa acttggacgt tctttgatgc ggaaataact
     9241 ggattttatc gaaattttca tatttccata gaaaaaatcc agatcgaagt aatcattcat
     9301 tcgtcctttc cctaaaagtt gacgtacatc atagctgata tcatctggtt cgccgaaata
     9361 agaaagaact tgatctactg tatgacaagc atgtccatat aaatagctgt tcgcagtaga
     9421 aaattcatgc gtattttctg gtacttccgg tctgaaataa tcataatgca tttctacttc
     9481 taaaatatcc cctaattttc cgctttcgat tactttttgg actgtcaaga agtcagaatc
     9541 gaaccgtctg ttttggtaac attggataaa tagacctttt tcttttgcta atgcaaataa
     9601 ctcttctgct tcctttgacg tttctgcaaa aggtttctct actagtacat tcttaccatg
     9661 ttcgagtgcc atttttccat attcataatg aaattgacta ggtgtagtga tgacaactaa
     9721 ttggatctca ggatcatccc aaagatcaga cagctgatca gtatattcga tgtcttcgaa
     9781 tgttttccac actggttcag atggggaaaa aatttttttg atattgattt tgttcgtaag
     9841 ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt ccattgccaa gataagcgat
     9901 tgtaagcatg agtggtgcct ccttttattg atgttttcag tatatcctag aaaagcgcag
     9961 aaagtattta taatagctat tctggtaaaa aagttcagaa aaacacttgt tatcattcgg
    10021 tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa aagatagtag cggaaccaag
    10081 tgtttatcag taagatagaa tttggaggtc agatcatgct tattgaagaa aaactaacaa
    10141 aacaagagtt gtttaccacg acagagaaac ggattgtcga ttacatccgt aggaatatcg
    10201 aagctgcagt ctacatgacg attgaagaat tagcaaaagc tacgtatact tcccattcag
    10261 ctatcattcg tttatgcaaa aaaatgggat tcagcggatt taaagaattc cgtttcgagc
    10321 tggggcggga agttcatcaa ttgattgctc agttcaatca aacgattgat cctaattttc
    10381 ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc
    10441 agtcaataaa aaaagcacaa ttgcagattg aacatgaaga tttagatcaa atcggtaaag
    10501 tattgaacaa ggcacagcga atctttcttt ttgcaaaagg tgattctcaa atcacggcaa
    10561 ggaaattcca gaataaaatg gtcaagttga ataagttttt gatcatggct gaagaataca
    10621 gtgattcttc ttggaatgca gccaatttga ctagcgagga ttgcgccatt tttattagct
    10681 acagcggtcg catccatcat tatgaacgaa taatgaccta tttgaagcat gtagaggccc
    10741 ctaccttgct gattaccgga aatcagcatt cagaaatggc caaacaggct gatatgtgtc
    10801 ttgttatatc gcaggaggaa tatgattttg ctaaagtagc gactttctcc tctcaaattg
    10861 ccttcgatta cgtattaaat acgttattct cagtgatata ttctcaaaat tttgaagaga
    10921 atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa aggccttctt aaagaatatc
    10981 ggaaaactta agaaaagatc ctcatttagt aataagaaag atagtgtttg ataggctctc
    11041 tttgatatca tcataaaaac aggctgaagt tttctttttc aaacctcagc ctgtttgttt
    11101 acggaacgat tcgaatagat atattataga aatgaggaat agctaacttc tgtttcttat
    11161 tttgaacgat tgacgaaaaa gatatataag aaggaggaaa atcagtatac atgcagctaa
    11221 caatccatac aggaaaaatc gtttgttctc ggatactttc tgtacttctt tttgaacttc
    11281 ttttgcatga taaggaaccc ggtgccctct gacaagtaag cgatgactgt tgaccatata
    11341 aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt tcgattctca gatcttttgt
    11401 atcagtcggt tctaccgtct tgatctgatc gacttgataa gcgagtgtcc gtccattgat
    11461 ttcgatatag aaagaatcac ctaaagtcag tttcggtaag tctgtgaaca actttgcttg
    11521 aggcagtcct cgatggctgg aaataacggc atgtgtattt tctccccccg taggataaga
    11581 cgttccttcc aataatgagg cacctttttc tagcagcaca gtattcgttt catcaaaaat
    11641 tggcaaatgg acattgatct cagggatcgt caaaataccg atcgtatgtt tctcaaaata
    11701 ggttctatcc tttttcgcag gttctttttt cttagtaaag ggatctgcac ctggatttcc
    11761 accttctttt gctaattgct ggtttttctt tgtcatgttt tcttggattt tagccatgac
    11821 ttcttcattt tctttgaccg cttgttgctg ataatgagag atgatctgct gatccagata
    11881 attattcagc gcgtcgctaa caaatggata agctagtatc cctattcctg agagaagcaa
    11941 gaaaagcatc agcagatcta gaagtttcct tttctttggt ttcatttgtg ttctccttgt
    12001 gatgactgtt tgataataaa agaaaaagga gcatttgcgt aatccgctgc tcctttttcc
    12061 aattcttgca aaaagactaa atctgactgt gcttgcgtct agcaaagtac agtccagcaa
    12121 tcagtagaag gactactcct gcaccgatat acacatagat tcccttaccg cctgttgaag
    12181 gaagtgtacc tttgtgttta tttggtattt tttcaggaga aatcaactgt cctgctgtta
    12241 cataagattg ttcatcgata gtaaatgcta cacggtttgt taatggaaca tatttttctg
    12301 gcgctttcgt ttcttccaga tagtacgtgc catattttag acctgtcaca tcgattaaac
    12361 cgttacttgt ggttgtaaaa accgttgctg attctttcgc cgatacccag ctgacggctt
    12421 ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact atcttgatca cgaacgacga
    12481 attctgctcc agcaagtgtt tggtctgatg taacgtcacc atctacttta acaaatcgtt
    12541 tgcccccagt aacgacatcg actgacggtg gtgtttgatc atttgtatgc ccgttatcga
    12601 catttgcttg gttagaaaat cctttggttg gatctgcttt ttcgttcaaa tgcatatagt
    12661 aaacgaattt caatgtaccg ccaggagtta atgaaggaat ataattcgga tcaactgaaa
    12721 ccgtgaatcc gtctgtttgc tcagtgacag aataatttac tgggtcgatt tctttatttc
    12781 catcatataa ggcataagca tacgttcctg aagaatcatt atcaaatgtt aaagcagcgt
    12841 catgagtatc gataagtttg aatgttgtgt acttgttttg cgttccttct ttatcagcga
    12901 tacctaatgg gatattgaca gaaatctcat attgaatttt ttcaccgatt gcgacatctt
    12961 ttccattcaa ttgaggtgtt gttttatcaa ctttagacgt atcatttttg atggtctttt
    13021 ctactggtgt ttggctattt gccaggatct caaaaggcgt gattgtttgc ttttcaatca
    13081 tttccgcatt atcaggagct tttacttctt ctaaattata ttttccaact tctaaatgat
    13141 taacgatcaa ctggcctttt tcaatagatg cctcggcaaa gtcattgttg ccgatgtcat
    13201 aagaatgacc agtaatgaaa tgtttggctt tggtttgatc agttgtccaa gtgtacaatc
    13261 catctgtgac actttggatg tattttttga cgcttggtgt tccttcttct ttagaaataa
    13321 taaattctgc tccatttagt gcttcgtttt cggcagtacc gatttttgta actttcaacg
    13381 ttccatcatt accgactgta tttttagggt agagatggat agtatctagt tcttctgtcc
    13441 cgtatttata agagccatct gcttgtttga tcatctcata tacagggaaa gctaaaacca
    13501 tgtttgcggc agctgacact ccgtcttttg gttcttcttt gatcgtgtag actgcatctt
    13561 tcccattttg ttttttaggt aaagataaag tgacatttcc atcagcatct gtcgttcctg
    13621 aagcaactgg tgtaccagga gtcaaagact ggactgcttg ttttgctgca tccacggacg
    13681 ctcctttatc tcgttgcgca taaaattctt gagtgacgtt ataaactgaa aatgaaatat
    13741 cggctaatcc ttggtattga tcgaattcgc tcatttcttt cccgctgttt tggattaaag
    13801 gatcgggtaa atcagtcatt ttctttttat gaaggatgac ttgagctgct gtctcctctg
    13861 caaacacttt ttttgcgccg aagctgtttg tgagtaatgg taaaataagg aaaaggactc
    13921 ctaacataac gtttattttt ttatgatttt tcattttttc actcctgttc tttttttgat
    13981 aaacaaccag attgcaaagc ttgcgaatac cataccaaca acgcctaaaa gtgtattttt
    14041 cgtttcgttt gtttttggaa gaaatggacg gctattttct tttttattga ctactttcaa
    14101 tatactgtcc gcctctttat cctcagaaag aaaagttgaa atggtaaacg gaatttctgt
    14161 ttcacttaaa atataaccag aaggtgcttc gatctctttt aatcgataac ttcctgtttt
    14221 caacccggaa attgaaaaag cgccattttt atcggaaatc aggtgaagcg catctgattc
    14281 gttctttgtc cacttgtatc cgttggctgt ttgctggagg taatttcctg ctttgttttt
    14341 aataaggaat actgcgccag ctaatcgttt atctggttta tttgtctcga ttttgacaaa
    14401 ttgcttactc cctgtatata cagagacttt ctttgtactg acaccaaaac caaaatccag
    14461 tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta ctgcttagct gcatctgata
    14521 agaaatggtc agtttttttc cagcatattt ttccaacgaa gggatcgaaa ataaaacatc
    14581 aaatccgtga ggtttcttgt gtatgacaaa gccttctgtc agcttttctc cattcgatga
    14641 aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc gcactatctg acaattcgaa
    14701 cttttgatag tcaaggatat ttacaggaat tgtcgtatgc aaggaataag tgattttttc
    14761 accaatcgta aaatcatttc taggctcttc taataccttc tcaaaaggtg gatcagggta
    14821 gtcgttttct tcatttttag gatacaaatg gatagttgaa agtttttgat tgttttgtcc
    14881 atgtacagga agaacaacta ccatattctc tgccttttct ttgacgactt ctggtacttt
    14941 ggattcaatg aaaagatacg ctttatctct tttttcttga tctttagcgg ccagagaaaa
    15001 agaagcgata ccatcttcgt tgttaagagt tgtagttgtt gcttctgcgg taaacatacc
    15061 ggaagacgca ccgttttttg cgatctctgc ttgtgcttct tctaccgttt tgcccttttc
    15121 ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg aatgtgacac catttaatcc
    15181 tcgatacgtt tgtaataaag ctttttcttg tccgtcattt ggatgatttt tcggcagttg
    15241 cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc atttctgttt ctgtttctgt
    15301 tgctacctga gtaaaagctg gtttaaatag taacaagaag agacacatac taagccaacc
    15361 aagttttttc atatttagct gccccctctt tcttttttca aaaaaagata ccaaatagaa
    15421 aacgcacaac caatcatccc tactagatag attcctaaac ggccaattcc tcccgtttca
    15481 ggtaatggta cttttgcctg attcgtgatg tctaaagaaa tctggttgtt tttggctcct
    15541 ggtgacagaa cagattcatg atcttgtcca tccacttgaa ttgacccatc ttcgtgtata
    15601 actatagtaa ctggctcttt tagaccttgg tatccttctg gtgtaaaagt ttcggtcagc
    15661 gtataagttc caggttttaa attttcgaat agaaaggtat ctgtttcttg tccatctttt
    15721 ggcgattcta agtccatttc tggtccctgc agtctgaatt ttgctccttt taatgtctgt
    15781 ccgttgtctt cttttttgtg taccgttaga tcaaacgctt ttagtcgatt ttgatgagtg
    15841 agcgtccata cattatttgt catctcggta ttttttcccg tgtaaatgat ttttccatat
    15901 ttatcgattt ggaattcata atttcccggc gaagtatcat atccggcagg accagctgtt
    15961 tcaacgactc gatatttacc aggtgtgagt gaatcaaaac tggcaagacc tttttcattc
    16021 gttgtttggc tgtcaattgg ctgataagta ccatttgctt cttttttctg tagggaaaat
    16081 gctgctcctt taagatttat ctctttgtcc gtcccttgca tcgcatactt acgtactgct
    16141 acaggaactt caacaaaagg attttcaata gtcaactgga tcgtatcatc ggaagtagtg
    16201 actgtttttc catcaatggt taccgtacca tcagaagcga tcttgatttc ccaagtagtc
    16261 tttttgctta atccatgccc ttctggagct tttgtttctg tcagcgtata ggtcatttct
    16321 ttttgcaatt tgacattttc tggaagagaa taggttccgt cgccatgatc tgttagtaaa
    16381 gtatcaatag aatctcctgt taatttgaaa acagcgccaa taagatcctt ttcacctgta
    16441 gaggaatttt tcgttatttt caagtttaac ggtgtgaatt gtttagtatt tttccatgtc
    16501 attgcatcta tttgttgaga atcgtatcct ggtacaggta attctttgat tgtttggtaa
    16561 ctgaatgctt gaccttgatt attgtattga ggtagtgata atatctcttg ataactttct
    16621 ccgctatttg cagataattt gtcaatgtct gcacgttccc acgtatttgt tgtatcttta
    16681 gctggtttaa tgattcgaat atatccgttt ttccaagctg cagcatttgt cgtatgttcc
    16741 cgttgaatct caaaagtaac ttgatctgga cgatcagcta gattgttgtc aaattcttcc
    16801 cataactttt tgatgtgaag actgactccg ggagctttag cagatggtat accgaattca
    16861 gctaattcat tggtatcaat gtttggctgg aaagttgtcc tgccgttcat ttgataccag
    16921 aaatttggat ggaagtcctc attttctgtt tggattctca cttggtaatg gatttggatt
    16981 tcttggtctt ttcctaaata gatctgattg ctcttgatgg tatttccttc tatggaaata
    17041 gatggagtga ccgttgtagg acttgtcccc acactcttga ctgaaagtgt accaggctga
    17101 taaatgaatg gttctgcgat tggatcattt atttgtccat tgcttacagt tgctgagatc
    17161 tgtacagctt ttttggctag gtattcagag atatctgttg catgatcagc tgattcatag
    17221 taaagatcgc ctttttcatc tgatgaaacc atttgtcgca tacgagactc tacttctgct
    17281 tttgagagac cagctgccgg atcgctttga agttggatgc caagaccatg tatttcgatt
    17341 cctcgttctt tgagtgccat cgcttctccg atggttgcga taaacgtact gtcgattctt
    17401 ctggatagat tgttttggtc aggtgcatca tagattcttg agattagaga agtatttccc
    17461 ggccgatctt gcgtattaga aaactgagtt ccgtaataat tgctgcttga ttgtgcgtgt
    17521 acccgctgta ctttatagga aaatgttggt acaccatccg tcagcaaaac gatcactttt
    17581 ttatgtccat ttggaacgga tagcatgctt cctgcatctc ttagtgcttt ttgtgtaaaa
    17641 gtaccaccat ttgtccgtga aggtgtaatg gattttactt gatttttcac tgaatcaaat
    17701 gaacccatct gtactgcacc gttactgtag ctatatcctt cgcttgagta gccgacatat
    17761 cccatattga ttttgtctgt gataccgcta tctgctaaag tatcgacaaa acgatcgaca
    17821 ccaatcttta cttcaccgat ccgattattg tcgttcatac ttcctgacca gtctacgacc
    17881 aatacgagat caagaggcgt gatatctttt tgtacatttc cacgagcatt caaataaaca
    17941 tcaaacaatc cgggtgtact tgtttccttt gcatactttc gtatcgcata gtctgcttga
    18001 ttaccggttc ctccatattc gatatacgaa tgggtccgat cattaggatc tccattccaa
    18061 gaatttatac catcccattg atttgttccg tttttattgc cttgatgatc aagaacattt
    18121 gtatttcctg taggttgcca actggcttta ggataagtgc cctgatcatc tgttgtatat
    18181 tctgtaggaa tcgtttgatt agatggcaca ctcacagctg gcaaggataa tgtaggcaat
    18241 gtaaatgggc tgacagaatt ttcgttttct tttgatgatt cttcttctat ttcgtctggc
    18301 tttggtactg acacatctgt atctttctta ttttctgtct catcaataat gtcatattct
    18361 ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa gtgctggatc gattggaaaa
    18421 ctcagatcga tagtttgttt agtgtttgat gagatttcca acgtaatttt cccgctattt
    18481 acctgataag catagccatc tccttctttt ctttccacat cacttaagta attttcgact
    18541 agaccaattt ctttttctat cttttcagca ctggtgttat tcgcatttag cttgatgttc
    18601 agtgcttttt ttccagactg atccgaaatg acttcagctg aaattgtttc taaaactgtt
    18661 ggatcatctg tagtgtctgc tattgcagat aatggcacaa agtttgataa actcaatatt
    18721 atcagcatga aaataacttt cagtttcttc cctgtggtcg tcatttttcc cctccttttt
    18781 ctttttttct gttttgacgt gaatagtgaa aattattaaa ataaacaggc gttacataat
    18841 tttatttatt ttaatatgat aattattata cgaagggcgc taaagggaat cacaccaatt
    18901 ttcataccaa ttggtttcat ttatgaagtt gattccttcg attaatgagc gtttcattga
    18961 aagaaaaggg ttgagggaga aaaacatatg atcaggttgt tggaaggaaa ctatagaaag
    19021 atggaaaaaa tcattcgaat tctgtccatt tctgacaagt ggatgactaa aaaagaactt
    19081 gccaaacgaa ttggtagttc agaatctaca tttgtaaggt atatcgaaga gatcaaaatc
    19141 cgctggggaa cggatgtaac gattcaatct tcacaaaaat tggggtatcg tcttgaacat
    19201 ttgaatgtac cagcttattt aaatgttctg atggaaatgt cgcaaagttc tacaaacagt
    19261 cgtttattaa atgagattat tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa
    19321 atttccatca gccggtcttc ttttacacgt aagttgaaac aatgcaatca aattttagaa
    19381 agctatttat taaaagtgat cgttgaccaa ggttttcagt taactagcct taaaagtgag
    19441 ctccagttgc gtatctttgt tacctttttc tttttaagtt actacggaag acgcacgttg
    19501 ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg
    19561 aatttgtttc aggaagaaag cccatatgag caatctttct ttattatgta ttttgttgtg
    19621 catctcatga gagaatccca aggaactcca tttgaatgga aagctggaga ctcttatgta
    19681 cagttgataa atatcgataa aaaagacttc ttaatcttga aaaatctctt aggaaaattg
    19741 aagtacagca gctatataaa agtagtgaac tattttgtca atacgatttt ttcagaattt
    19801 tcggagaaag aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc ctactgttcg
    19861 ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa
    19921 atttattttt tctcccgtat tttcccatac gatacgactc atttgacgat ccgtgcaact
    19981 tactttgcac agcagatgca acaccgccag atacgtctat atgctggatt tcgtgcagaa
    20041 cttcaatttt attccactct actgaatcag aatctgattt attatttatc tagctatatc
    20101 ttttggatgc ttatcggtaa tcctgaaatt caccattata cttctgataa aaaaatactg
    20161 attatcagcg acttaagtct tcgacatagt caatatattg aagaatatat cagtgatatt
    20221 cttgctgtgc ataaaattca ctctgagaca actgctatta ctgaagatca gctgtcgaaa
    20281 tacaatcttc tcgagtatga tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt
    20341 cctcatatat tgattgatga tagtgtgtca ttcgcaaatg aagaagaact catccgctta
    20401 ttcgagttat gaataatcaa aaaacagcta tgtataaacc accctgataa aaatttcagg
    20461 acaaatacac atagctgttt ctttttattg tattccttca gcaatccaac gagatttata
    20521 gcgaaagtct tttaacaact cttcttttgt ccaattattt gcaatgagat ccgtcaaagt
    20581 gttcattacg agggtcatgg aaaagtatag gaaactaact gcttcttccg acgattgaat
    20641 ggagaaatga ttgattctta ataattccag ccatttttcg ttcattggag aatcagccga
    20701 tagttctcta cgtttggttg tctgctgatc atttcgctgg gttaacaagt ataacctttg
    20761 ccaatagata ctttcgtgat ctaagtcgca gcaccactct aaatattttt cgatccccaa
    20821 aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata tcttgatgga ctatgtgaga
    20881 actgcttttg atcgtataca aataagcatc ttccaaatct tgaaaatatt tataaaaagc
    20941 accgcgcgac atccccatct tgtcaacgat ttctgagact ttgacttggc tgatgtgctg
    21001 tgagcaaaat atctccagta ataggtcatc gattctctgt ttctttgcgt tcgataaatt
    21061 gaaaaaagtt tcttttggca tccacatctc tcctttatct ctgattatac gtggatagcc
    21121 cttcattgtc atgaaggatt cattccttct ttttctcttc ttctgtcaag tacccatctt
    21181 ccattcgata aacgcgatca ctccattgaa tcatacggac atcatgagta accataatag
    21241 tcgctttttg tttctcatga gcttctttaa ctagtaattt gacgacttca tatgcatgat
    21301 ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa gcttggctca ttatataaag
    21361 cgcgagcaat tgccactctc tgtctttctc caccagaaag gtctttagga taactatttt
    21421 taagtgactg gatatctaaa gattgcagca aggaatcgat ttttgttgaa tcttcttttt
    21481 ttgaagtaac ttggtcaact agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat
    21541 tagagctttg gagaataaat cctatttctt taaagcgtaa atctgctcgt tttttttctg
    21601 gaagctcagt tagtgagtga ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc
    21661 ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg atgctgacga
    21721 attctccttc ctttacataa aaatcaattg ctttcaaagc tgagaccttt gtatgaccag
    21781 aaccaaatgt tttggtaact tttttcatcg ttaatatttt tttcattttt attaacctcc
    21841 tattgctgta attgggtcta ctttggttat cgtacgtatc gaaaacaatc cgcctagcat
    21901 tgcgacaatg atcaaaatcc cactatatag caaccattgt gaccaaaaaa cagcaaaagg
    21961 catcgcgctt ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc
    22021 taccacccca acaataaagg actgtgctac taaagattta gcgatgaaga aatttttgat
    22081 cccttgtgct ttcattactc caaaaatagc agtcttctga agtgtcatca cgtacatgaa
    22141 aataccgacc acggcagcaa ccactaagaa caaaaagtat atcatcgcat ttaaggtcat
    22201 attttgggca gaatagccag gaatactttc aatgaatgtc ccaatagtta ataactgtag
    22261 acccttcgat gctttttctt tcgaaatttt agctgaatcc tttattacga tcccattgat
    22321 cggctgttct tcttcagaag caaacggctg ttgtccatac ttcaaagcag tccacgtatc
    22381 tagggacgca tagatgacgg gagaaactgt gtaataagtt tcaggaaaga taccgacaat
    22441 cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct ccaattttat agcctgaatc
    22501 agccagattt tgagagatga tgatttcatt ctttgtttta aattcatgcc ctttcgtgag
    22561 ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt ctttgttttt
    22621 tcctttgatt gctccgctgt acaatccgat tggtgctttc tctcctcctt caatgtattt
    22681 cagatctcct cttgttaatt gtgaagctgc aaatacttga tttgcatcct ccgacaaaac
    22741 aatctcttgt gcgtcccaat catcgattgc ttttttaaac tcttctgaaa gtccattcgc
    22801 tagtcctgac aacatgaata cgacatacgc aatcaagaac ataataccga cgatcaagcc
    22861 atatcgtaat ttagaatagc gaatttcttt gatagctaga aacatttgtt tttcctccct
    22921 atgatagatt gatagtgaca aaatgtcact tgttgaatag tgtaactcta aagtgacaat
    22981 ttgtcacttg tgttgttttt ttcttatcaa taaattgtct aagacatctt tcgataaaac
    23041 cttaacatga gtcataaaaa atttatttaa gccttattta tgctttccat gataaactaa
    23101 agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt agtagtagaa gacgataaag
    23161 tattatccaa taatataaaa gaaatactcg ctgcaatcgg cgagataaca caagtttatg
    23221 atggtagtga aggactgttc gagttggaaa gtggagttta tgatcttgca atcttggatt
    23281 tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac
    23341 agactccagt attgattttg actgcaaaag atagtttgga agataagatt gaaggatttg
    23401 aacgaggtgc agatgattat ctaacaaaac ctttctatcg agaagaactg atcatgcgcg
    23461 tgaaagcatt attgaaacgc tcgctaggtc tctttgatga aaatgttctg aagtataagg
    23521 atatcagttg tgatttaaaa agcaatgaag tcttgtataa agaacaagtt ttgcccatac
    23581 aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa taagggaatc attttgacaa
    23641 aagagcagat atttgaccgg atctggggat ttgattctga tacgacgatt acagttattg
    23701 aagtttatat gagcaactta cgaaaacatc tgcgaagtac tggattggat aaagaaatca
    23761 aaaccttacg taatgtcggt tatatactgc aaagggtgtg atgcaatgaa tggagatatt
    23821 acacgaaagc aacgtagtcg cttttttgcg acaaatgtag cagcatttgc ttttatcttt
    23881 tttcttttgg gattcattac tttacagata ttaaaccaat ctgcatacag agaaacagat
    23941 ctgtcattag agaaaataaa ttttgattct cgaacagtac aattagagat acaaagatat
    24001 cagcaaggcg atcccttttt aaacccgact caaacggaac aaaacagaac aaacatggaa
    24061 cagccattaa tggaaccatc aaatgaccgt tttaacactc aagtgatctt gtggtctaaa
    24121 aatggtgaga tattgaataa agcagctatt ggtgggcgtt tcaaccaaat caaagaatta
    24181 aaaatggata cgaaaaatct gaataccatc caaaatattg aattaaatga agacagtggc
    24241 tcatctgccc tctctttccg ttcgattacg aaaaaagcgg agacgaatca agatggcgtt
    24301 gcctacatcc aaattttaga aaatacaaat caaatcaaga attcattagc gacttttcgc
    24361 acgattatga ttttgtgtat gattgttttt tggattctct ctattggtat cagttattac
    24421 ttgtctagtc taagcatgcg ccctattatt ctttcttgga gaagacaaaa agaatttgtt
    24481 gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag tttggaacat
    24541 ctttttacca agcctaatca tactattatt gaagaatccg aaagcatcgc tcaagcattg
    24601 tcagagacga ggcgcctcac tggattgact tcggatttgc tgacaatcgc tcggaatgat
    24661 tctgatcagc agctgctttc aaagcaaatg atcaatacac aagaatatat cgaacaactt
    24721 gttaaaccat ttcaagagat ggccatcata gatggaaaag agtttattct ccataacgat
    24781 gcaacattag aagtaccaat tgatgaaaaa aaaatccatc aagttttggt tattttgctg
    24841 gataatgcat taaaatatac gaatccagaa gataagatca ttttggattc atctgttaac
    24901 cataaagaat ggctgattca cgtaaagaat actggccctt ctatcagtga agaagatcaa
    24961 gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga gactggcgga
    25021 tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg acagatttct
    25081 gtatccaatt atcttccaca aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa
    25141 aaataatgct tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt
    25201 tgcgaataaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttctttaagc
    25261 tgaccacttc tgtcacaacc tctttagctt acgcttacga ttttttattg cggtcttcaa
    25321 tgtgtacatc accgcttgtg tcaatatcca attcttctcg gctaacatct tctgtcactt
    25381 tttcaacatc ttcatgttct tctttatgaa tcccaacttc ttcacgaata actgtatgtt
    25441 tgcttacatc tacttgctct tctttgattg ggatagtaat cgtgtcatct tccaattttt
    25501 cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg atttcttcac
    25561 gtttgaccgg tacttctacc gtttcagtat cgttgaccac atgtttgtgg atgtcgactt
    25621 ctccagttgt cacatcatgt gtatttacat ctaatttttc ttcttttagt tgaattgtat
    25681 catcatctag atttggtttg cgatcgatgt cttcagtctt gcttgtaccc attgtatcag
    25741 tctcgccagg tacgtccatc ggatcagttc ctggtgtcat attcaatgga tccgttgtat
    25801 tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca gtttctaatc
    25861 cagtccccat tgtgcttgct gggctaacag tatcttgtgt atccattgta tcttctgttg
    25921 tttctggctg acgatagcct tttactgtga tgacaaattt tccatcagaa atatctcctc
    25981 tatattgata taaaggatca ttttctggtg tagccgtctc agaatcatag gtatcaaaag
    26041 aaaaagtatc ttttattttt tcccataaag aacggtcttc ttcaccatcc atccgatttt
    26101 catctgtttc aacatctata ccagacaatc cgtcaaatcc tagtctagat gctgcttcat
    26161 cagtagtata aataacgata tcatctcgtt catacccttc gttacgcaac cgttcaatta
    26221 ctgataatgc ttcttccctc gtactatagc ttccgatgac cgttaaattt ttctcttcca
    26281 tgtaaattcc tcctagcaaa tag
//
LOCUS       DACDKL010000020        25382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDKL010000020 DACDKL010000000
VERSION     DACDKL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.020"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(92..274)
                     /locus_tag="IV329_000666"
     CDS             complement(92..274)
                     /locus_tag="IV329_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939872.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            249..1688
                     /locus_tag="IV329_000667"
     CDS             249..1688
                     /locus_tag="IV329_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8939873.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(1695..4247)
                     /locus_tag="IV329_000668"
     CDS             complement(1695..4247)
                     /locus_tag="IV329_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8939874.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(4361..5692)
                     /locus_tag="IV329_000669"
     CDS             complement(4361..5692)
                     /locus_tag="IV329_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8939875.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(5658..7136)
                     /locus_tag="IV329_000670"
     CDS             complement(5658..7136)
                     /locus_tag="IV329_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8939876.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7441..9276)
                     /gene="tdc"
                     /locus_tag="IV329_000671"
     CDS             complement(7441..9276)
                     /gene="tdc"
                     /locus_tag="IV329_000671"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8939877.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            9746..11173
                     /locus_tag="IV329_000672"
     CDS             9746..11173
                     /locus_tag="IV329_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8939878.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(11265..11630)
                     /locus_tag="IV329_000673"
     CDS             complement(11265..11630)
                     /locus_tag="IV329_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8939879.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(11627..11992)
                     /locus_tag="IV329_000674"
     CDS             complement(11627..11992)
                     /locus_tag="IV329_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8939880.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(12184..12432)
                     /locus_tag="IV329_000675"
     CDS             complement(12184..12432)
                     /locus_tag="IV329_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939881.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            12431..12559
                     /locus_tag="IV329_000676"
     CDS             12431..12559
                     /locus_tag="IV329_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8939882.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            12616..14310
                     /locus_tag="IV329_000677"
     CDS             12616..14310
                     /locus_tag="IV329_000677"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8939883.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            14377..14583
                     /locus_tag="IV329_000678"
     CDS             14377..14583
                     /locus_tag="IV329_000678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939884.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            14961..16094
                     /locus_tag="IV329_000679"
     CDS             14961..16094
                     /locus_tag="IV329_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8939885.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            16125..16445
                     /locus_tag="IV329_000680"
     CDS             16125..16445
                     /locus_tag="IV329_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939886.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(16474..16938)
                     /locus_tag="IV329_000681"
     CDS             complement(16474..16938)
                     /locus_tag="IV329_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8939887.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(17070..18323)
                     /locus_tag="IV329_000682"
     CDS             complement(17070..18323)
                     /locus_tag="IV329_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8939888.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(18352..18669)
                     /locus_tag="IV329_000683"
     CDS             complement(18352..18669)
                     /locus_tag="IV329_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8939889.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(18695..19015)
                     /locus_tag="IV329_000684"
     CDS             complement(18695..19015)
                     /locus_tag="IV329_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8939890.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(19479..20894)
                     /locus_tag="IV329_000685"
     CDS             complement(19479..20894)
                     /locus_tag="IV329_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8939891.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(20911..22227)
                     /locus_tag="IV329_000686"
     CDS             complement(20911..22227)
                     /locus_tag="IV329_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8939892.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(22377..23075)
                     /locus_tag="IV329_000687"
     CDS             complement(22377..23075)
                     /locus_tag="IV329_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8939893.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(23364..24353)
                     /locus_tag="IV329_000688"
     CDS             complement(23364..24353)
                     /locus_tag="IV329_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8939894.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(24433..25266)
                     /gene="msrA"
                     /locus_tag="IV329_000689"
     CDS             complement(24433..25266)
                     /gene="msrA"
                     /locus_tag="IV329_000689"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8939895.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         7854 a         5231 c         4282 g         8015 t
ORIGIN      
        1 tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt gctaccatct
       61 actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg gtatcatttt
      121 tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa acaatgattg
      181 aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa tagaagggag
      241 tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt cgacgtcatg
      301 tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa ttagctgaaa
      361 tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc tctcagctgc
      421 ccgaaggctg ggacatcatc agcgatcaag caggaatcag tttgcaaaac cagcaaaatt
      481 tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc gaactgctga
      541 aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac catgggcttt
      601 cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca agttacgact
      661 tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt gcgattcgta
      721 ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc gaggattatt
      781 cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact gaactagcgg
      841 ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc cgtataaaag
      901 cgaattgccg attagattct tctatccata ccaacagcac actctcctcc ctgtatgact
      961 ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact gagctttctt
     1021 ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga caagaaaccg
     1081 ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta gaggaactag
     1141 cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac ttggctctcc
     1201 tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt caataccatc
     1261 gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa gagaatttac
     1321 tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc caactcttgt
     1381 ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat gtctatttta
     1441 ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa gattacttag
     1501 gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt tcttttgaaa
     1561 aaacagatat cttgatttcc aatttccctc tagattctct agatatccct attgtataca
     1621 tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact ttcaaaactt
     1681 ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt gcgaaacagt
     1741 ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac atttgctcca
     1801 atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg gccgactggt
     1861 agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa aagcgctttg
     1921 ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt gatcatgtaa
     1981 acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct ttgcccttca
     2041 aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata gatgatccga
     2101 aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg cttcatgatt
     2161 tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt taccatattg
     2221 acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag tgcccaaatc
     2281 aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt ttgcaaatta
     2341 tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt atcatctcct
     2401 aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat agcaattcct
     2461 acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccgacgatt
     2521 tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt cgttcgagca
     2581 aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag atccaaatcg
     2641 atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat cgcttttgct
     2701 gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg gcattccttt
     2761 acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc tgcaaaagtt
     2821 aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt ataagcaaat
     2881 cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca tgcttcttgt
     2941 tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt gacgtaaatc
     3001 accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga actgaacgga
     3061 atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg aatcagcgca
     3121 acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt ttttaattcc
     3181 tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac atccacaact
     3241 gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt catagaacct
     3301 aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat ttcatgtact
     3361 ccaacggata gaatcatggt caaaacagct ggcaatcctt cgggtaccat tgacaccacc
     3421 aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa tgtcgtaaaa
     3481 ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt taatcggttg
     3541 atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg attgatttta
     3601 ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca
     3661 agcgttcctg aaaaagccat atttttctgg tcgccgatcg attcttcttc tgtcaaaacg
     3721 gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc cactaataaa
     3781 tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg taagacaatg
     3841 acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt tgatagtact
     3901 atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc ttttctttct
     3961 tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact tccttcaata
     4021 tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat cagcagatca
     4081 gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt tatttcatta
     4141 tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc tgttgatgtt
     4201 tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct
     4261 gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag
     4321 gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata tttctattgc
     4381 ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa aaaggaataa
     4441 agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac accaacaaag
     4501 caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat tgaacatgtg
     4561 cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct gccaaaagct
     4621 gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca aaaaataaca
     4681 ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca caaaaccaat
     4741 ggggcaaact tttttctttt gctgtttgat ataaaatcct gctagtactg tagactgcac
     4801 tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct gctccgcctt
     4861 ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct aaacaatgcc
     4921 atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga atacgccaag
     4981 gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg ccaacggtta
     5041 agccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag actaccattc
     5101 cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg attccgatag
     5161 actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa aggaaaatcc
     5221 ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg actgcaacaa
     5281 ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct ggtacatgta
     5341 caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca gctccaagcc
     5401 agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa
     5461 cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc atggcccgca
     5521 tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct ggccctgctg
     5581 atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca cctaatgcga
     5641 ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc atcaagttca
     5701 cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt ctttattttt
     5761 tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc aattagattg
     5821 atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc aaataaaaca
     5881 ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat tctaaatggt
     5941 cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat gatgacatag
     6001 acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg attgacgcca
     6061 ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt caaaataacg
     6121 gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg atacgcacct
     6181 tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga taattggact
     6241 gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg caaaccaaga
     6301 atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg tccttttggt
     6361 acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa aattaacgcg
     6421 gcaaatactc cgcgaatata cgttttaact ggattttcca atcgagtgat atacacagag
     6481 gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa tgaggatgca
     6541 gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg
     6601 atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa aggcacgtac
     6661 aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt caaattcaaa
     6721 agagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct gttcaaacca
     6781 agcggcacat tatcgataat attaccaaac aacggagcga gtgctgaagc gaccatcacc
     6841 atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca tttttttcct
     6901 agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc agcaaattca
     6961 ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat gtaaaaaatc
     7021 atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac aagggcgcag
     7081 gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt
     7141 attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt caagaacaga
     7201 aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat ccattgaacg
     7261 atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga acgttttata
     7321 aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat tgttttgatg
     7381 aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca aaaatcagtt
     7441 ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttttt ttgtataaaa
     7501 attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta gcagagtgat
     7561 tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa aacgaatcgg
     7621 agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc gttcaccata
     7681 aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt cattgatagc
     7741 gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa aatcaggatg
     7801 attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat gatcgagaaa
     7861 ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat atccggtcac
     7921 attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc cagcttttga
     7981 tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt caaacacata
     8041 tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga ttcctccagc
     8101 agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg cttctgaaat
     8161 agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga aaacatgatg
     8221 ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt cgtttagaaa
     8281 cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat agatcccttc
     8341 atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt gaccttcttc
     8401 tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgtccag ctaattcacg
     8461 aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg ggatagcaac
     8521 catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat agtgttttgt
     8581 ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg aagaagctgc
     8641 tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac
     8701 agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca gcacttcctt
     8761 gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa gattagcaat
     8821 agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg aagcaaaatc
     8881 ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg caacattatt
     8941 tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg tttcagcatt
     9001 catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg
     9061 actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat ctggagtgag
     9121 cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc tccatccaag
     9181 atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt cagctttgtc
     9241 gcctataaag acggccttga tatccatatc tttcattttt cttccctcca tattttgttg
     9301 gtcaatgtct tgcagataca tgtgtttatc tgtaacacct cttcactata gcgccaaaaa
     9361 tgagaaattt ttttttataa catcaaatcc acgtagattt ttacaaacaa aaataaataa
     9421 agtcggattt ttctgataat tcaacgcaaa aaagcgaaca atttttcaat aaatcagtct
     9481 tctaaaatag ataacctaaa aaaagcgaac aaacaaataa aatgactgtc cttcattcac
     9541 taaacatatt tttatttttt tatattgtta aaaattcaca atttattttt taatagaatt
     9601 atctgttttc caaattttat aacataaaaa acctttttta acggcattta tatgcttttt
     9661 cagaaattaa ctgatgattt caaaaagaaa acatgtcata atatttccca tattgtgcaa
     9721 aaaaatacaa ggcggaggct ctactatgat tgaagaatat attgagaaaa atattttaag
     9781 acagctgttt ttatgtgggc aattctatgt aaataaagaa gtcaatctag aaaagctctc
     9841 caatctcctg catgtttgca aaaccacttt actcaatgat atcaataaca taaaaaaaga
     9901 atttgaagaa cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc
     9961 agaacatata cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa
    10021 aacttgtctg ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga
    10081 attcatctct gtcagcaaag cttattcatt aaaaaaacaa gtattagctt actttaatga
    10141 ctgcgggatc gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct
    10201 gcttcttaat gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt
    10261 ttttgaacgt gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga
    10321 taaagaaaat aaagaaatct tatcgattgg ttttgctatc agtttcctac gccagcaagt
    10381 gtgtgctgtg acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa
    10441 ttatgtcgaa tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt
    10501 cgcctttatt ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat
    10561 tgcggaagac tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga
    10621 actagttgct acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga
    10681 acgtgcgctg attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga
    10741 aaaattttat cttttaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt
    10801 ttccagctgg tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc
    10861 ttttgtgatc gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat
    10921 cgtcacaaat tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac
    10981 tttcgatttt caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc
    11041 gcagcagtcc tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca
    11101 atatgaaaat attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt
    11161 gcaaaacaaa taaggggctg tgacaaaaat catgtcacag cctcttattt gttgaagatg
    11221 cccatttctg taacaatctc ttgatacacg tcaggctcac aacctcactt attttggaca
    11281 tctaggggtc cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac
    11341 gccgctagtt ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct
    11401 gaaacaagaa tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg
    11461 ttctcgctta ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc
    11521 ttgattgata gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt
    11581 acaagttttt cgaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc
    11641 ctcttctttg tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc
    11701 gaatgataat aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact
    11761 cgatgatatc agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt
    11821 ttcttgtatc tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat
    11881 caaaaagttt gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat
    11941 ttaaaatagc aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta
    12001 cctactttct tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc
    12061 tatttaaaag ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccat
    12121 ttttctccct ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa
    12181 ggcttagtcc gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa
    12241 atttctaaaa atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt
    12301 atttacagga aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc
    12361 tatcatttgg cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga
    12421 ggcgtttttc atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct
    12481 tggcgctgca tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga
    12541 tgaattcgaa tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac
    12601 attggaggaa tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat
    12661 atggcagcgg ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt
    12721 tattcattgg atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa
    12781 tattggaata aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc
    12841 atgcttaatt accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt
    12901 actgagccag ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc
    12961 tttgacaaag cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga
    13021 ttcaataata aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa
    13081 gaaaaactag acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa
    13141 acaaacttct ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa
    13201 gaattgcgtc gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg
    13261 ggcgtcaatc gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac
    13321 ttgaaagaac aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa
    13381 gaaacaccga tgcaagatga aatcactgtg actggtttga aaattaccaa tacactgact
    13441 caagaagaag aatacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc
    13501 attacagatt cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat
    13561 ggtgcagctt ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct
    13621 gacaaattct ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa
    13681 aaccatttgt tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg
    13741 aattcattta tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct
    13801 attgtcgtgc atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg
    13861 ggttatttcc tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg
    13921 aatggaaaag aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca
    13981 gtgaatattc gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc
    14041 tatatgcctt atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc
    14101 attcctaaac attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag
    14161 atgatcttta ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg
    14221 ggtattccta tgtcccgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta
    14281 gctcgtgccg caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat
    14341 caagtaataa gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta
    14401 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agctgccctg
    14461 catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac
    14521 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt
    14581 tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt
    14641 gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc
    14701 tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg
    14761 gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac
    14821 acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa
    14881 ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga
    14941 atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa
    15001 tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag
    15061 caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa
    15121 aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc
    15181 aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc
    15241 atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg
    15301 gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc
    15361 taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg
    15421 aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag
    15481 gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc
    15541 aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc
    15601 gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac
    15661 aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg
    15721 taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa
    15781 gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc
    15841 aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc
    15901 gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg
    15961 caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat
    16021 cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg
    16081 ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac
    16141 gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct
    16201 tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg
    16261 ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca
    16321 cctattttta tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa
    16381 ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg
    16441 catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag
    16501 acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc
    16561 ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc
    16621 ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac
    16681 aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac
    16741 gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact
    16801 ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca
    16861 ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat
    16921 ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat
    16981 acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aaacggaaaa
    17041 caatcacttg ttctccgttt tttgattaat caagcatcta aagcttcttt tcttctttct
    17101 tggatcgtag ctaccttgac gaacggtaga tagatcaaaa tagaaagaac caaattgaca
    17161 gcggctagta caccacctgc aatacttttt gttgccaata caccgccaat aattggtgga
    17221 gttacccatg gaggcataac ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa
    17281 gcaacactaa ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac
    17341 acgataggta acccaaacat cgttggttca ttaatattga agacacctgg cgcaatcgac
    17401 aagtttgtta caaccatata tggtttgttt ttacgtccta ctaaataaat agctaacagt
    17461 aaacctaaag ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc
    17521 acgataaacg gtgcgctatg tccagctgcg attgcattaa cgtttgcttc aatggctggc
    17581 gcattgattg tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc aaagaaccat
    17641 aagaatggtg tgataaatgc aagcagtaag gcagatggat aagagtttgc aagtcccata
    17701 aatggttctt ggaccactgt atagaaagaa cctacgatat catcaacacc gaatgcagca
    17761 aaaattgcag caactaaagc aaacgcagag atagtgatca ttgctggtag taatgaagca
    17821 aatgcttttg caactgccgg tggcacacca tcaggcattt tgatgatcaa tttttcattt
    17881 ccgcttaaac gtacaaagat ttctgttgaa ataatcccaa caataatagc aataaacaaa
    17941 ccgtttgagc tcatacctaa tgctccgcct aaagcgaaga aagagcctaa agaaatcaca
    18001 cctgcggaaa caccgtcttt tcggtagccg ttagctaaat gataagcaag caagaaagca
    18061 atcaagacag aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat
    18121 gattcaccac caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg
    18181 atcattactg ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga
    18241 atcgcaacta aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc
    18301 ataaatgcaa caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt
    18361 caatcgacga tacagattaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat
    18421 cagatgatct tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc
    18481 ttgtgtcagc atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt
    18541 gattttttca tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc
    18601 atcgcttttt gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg
    18661 atcttccatt tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt
    18721 ggtcgagtac tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag
    18781 gaatgccttt aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc
    18841 ctaaaagtaa aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata
    18901 cagcaaagat gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta
    18961 ggagacttgt actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa
    19021 acacgttcct ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc
    19081 gctttaaaaa gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa
    19141 aagaaagcgc acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca
    19201 caaaaaaagt tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag
    19261 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg
    19321 ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa
    19381 tcagctgacc ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata
    19441 accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc
    19501 aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt
    19561 ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc
    19621 attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct
    19681 tctgttgctt tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct
    19741 tgcacgattc ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct
    19801 gttatcagcc aagggatatt atgataattt tcttttagat acatggctat atcatacatg
    19861 attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag
    19921 acttcataat atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg
    19981 cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc
    20041 gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg
    20101 aactcttctg tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa
    20161 tcatacatat aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca
    20221 ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct
    20281 ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca
    20341 tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat
    20401 gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca
    20461 taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg
    20521 acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc
    20581 atttcactta tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa
    20641 aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc
    20701 acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc
    20761 caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt
    20821 cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga
    20881 atagtaattt tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc
    20941 tacttttaca aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat
    21001 aactgcaccg atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca
    21061 agggaccatt acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt
    21121 tccagtgata agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg
    21181 cataccaaaa ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt
    21241 gatttgtttc atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt
    21301 gactccagac ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg
    21361 tacttctttt ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg
    21421 ttcccgtatc ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc
    21481 aataaacatc aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg
    21541 tacgacattg taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc
    21601 cgcagccgcg atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga
    21661 aacattcgtt ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca
    21721 gcaataaagt tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc
    21781 tgtatattgt ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc
    21841 ataaacagtt ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc
    21901 atccacttta ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa
    21961 gaaacttagt gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg
    22021 caagaaactt atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt
    22081 gccaaaagca cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat
    22141 gatatacctt aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc
    22201 cataaaactc attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt
    22261 gtctattagt cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca
    22321 aatataaaac aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa
    22381 ttagtttctt tttctcctat tattttctgt aattctctcc gatttattgc catatggaca
    22441 gtcattgcat caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta
    22501 tgaatatcga tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca
    22561 attgattcat taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca
    22621 atcatcgtat gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa
    22681 gtttcaatct tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc
    22741 gctgccaaac ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc
    22801 atccgatcct gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta
    22861 tcactgacaa atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc
    22921 cgcacagctt ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca
    22981 ttcggcagtt tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca
    23041 gtagttactt ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat
    23101 gtattttttc gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa
    23161 ttgattaatc cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg
    23221 ataaaattca ctcctagcct tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc
    23281 aataacagac gatcttcatt cgattcttga tgtcatcttt tgtttttttt aatatatgga
    23341 taaaaagttt gattacatcg tttctattct agaatttggt cgattctcaa ttcttcttct
    23401 ggtgagaaat ctaaattatc catcattcga acactttctt gtaactgact taaccgactt
    23461 gctccgacca gtacacttgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc
    23521 gctaatgatt gtcctcgtga tacggcgatt gtttgcagtg ctttgatttt ttccagagtt
    23581 gatccaactt gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca
    23641 ggaataccat gtagataacg atttgtcagc aacccttgag caagcggact gaacgtgatt
    23701 gcccctagcc cttcttcttc taatactgga gatagatcgt cttctagtag tgcacgagaa
    23761 aacatattgt agcgcatctg atgaataatg aacggtgctt cctttctttt taaaatttca
    23821 gtcatttttt ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct
    23881 tgtcttacaa gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg
    23941 cgatgatgat agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa
    24001 cttgaaatga tggatttacg agagccccat tccccatatg gcccttccca catatgataa
    24061 cctgctttgc tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata
    24121 cgcccaaagt ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa
    24181 tgggtaattc ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca
    24241 taatcaccaa aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga
    24301 ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct
    24361 cctcctcatc aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt
    24421 aaagaaaagg agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac
    24481 tcgttgatag atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg
    24541 ttttttgtaa aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat
    24601 ttttgtgacg attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc
    24661 cgctaattct ttttgtcgtt cgacttgata aaaaatcact ggacgatatt gcttccctct
    24721 atcttgaaat tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa
    24781 ctcgctataa cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt
    24841 gtaaccacca cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga
    24901 caaaacagat aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc
    24961 ccctgcaaat actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc
    25021 atatttttgc tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg
    25081 cgtcagattc attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc
    25141 tttaacggat atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca
    25201 atctcgtgta ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc
    25261 atccatagat tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc
    25321 ataaagtgag gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag
    25381 ag
//
LOCUS       DACDKL010000021        25166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDKL010000021 DACDKL010000000
VERSION     DACDKL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.021"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            510..1154
                     /locus_tag="IV329_000690"
                     /pseudo
     CDS             510..1154
                     /locus_tag="IV329_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            1382..2671
                     /locus_tag="IV329_000691"
     CDS             1382..2671
                     /locus_tag="IV329_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8939896.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            2747..3628
                     /locus_tag="IV329_000692"
     CDS             2747..3628
                     /locus_tag="IV329_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939897.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYACQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            3618..4433
                     /locus_tag="IV329_000693"
     CDS             3618..4433
                     /locus_tag="IV329_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8939898.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            4449..5573
                     /locus_tag="IV329_000694"
     CDS             4449..5573
                     /locus_tag="IV329_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8939899.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            5551..6354
                     /locus_tag="IV329_000695"
     CDS             5551..6354
                     /locus_tag="IV329_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8939900.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            6805..7077
                     /locus_tag="IV329_000696"
     CDS             6805..7077
                     /locus_tag="IV329_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8939901.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            7061..7396
                     /locus_tag="IV329_000697"
     CDS             7061..7396
                     /locus_tag="IV329_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8939902.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(7575..8789)
                     /locus_tag="IV329_000698"
                     /pseudo
     CDS             complement(7575..8789)
                     /locus_tag="IV329_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8990..10264
                     /locus_tag="IV329_000699"
     CDS             8990..10264
                     /locus_tag="IV329_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8939903.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(10647..11375)
                     /locus_tag="IV329_000700"
     CDS             complement(10647..11375)
                     /locus_tag="IV329_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8939904.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(11353..12927)
                     /locus_tag="IV329_000701"
     CDS             complement(11353..12927)
                     /locus_tag="IV329_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8939905.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            13156..14094
                     /locus_tag="IV329_000702"
     CDS             13156..14094
                     /locus_tag="IV329_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8939906.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            14106..15017
                     /locus_tag="IV329_000703"
     CDS             14106..15017
                     /locus_tag="IV329_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8939907.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            15041..16513
                     /locus_tag="IV329_000704"
     CDS             15041..16513
                     /locus_tag="IV329_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8939908.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            16564..17175
                     /locus_tag="IV329_000705"
     CDS             16564..17175
                     /locus_tag="IV329_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8939909.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            17205..19118
                     /locus_tag="IV329_000706"
     CDS             17205..19118
                     /locus_tag="IV329_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP8939910.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            19246..22227
                     /locus_tag="IV329_000707"
     CDS             19246..22227
                     /locus_tag="IV329_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8939911.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            22224..23885
                     /locus_tag="IV329_000708"
     CDS             22224..23885
                     /locus_tag="IV329_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8939912.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            23925..24857
                     /locus_tag="IV329_000709"
     CDS             23925..24857
                     /locus_tag="IV329_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8939913.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         8106 a         4053 c         5143 g         7864 t
ORIGIN      
        1 ctgtctgtga ggacagcttt ttttgtgggc taggcatagc aaaaaggggt agtgacagtc
       61 cactgggagg gtgcacatcg accaatcgct agagaaacgc aaggtggtat ttgtgagaag
      121 atgacggaag aaatcctgta tcaaagactt ggttcaacga gcagaaatct gatagtaagt
      181 ctcggtcatt caatgggaat aagccattga acaccttgat ggtatcctgt aagaaagaac
      241 ctcaaggact ttcagcaaga agaaggaata gctgaaaaca aaaaaaggtg tagtaggtta
      301 aaagcgggga atgtagaact acttaattgg tgaaactagc tagaagggca aaatcgacga
      361 ttcatgagtt cgtaggaagg agcatgcaag atttacaact agtttactga aaaaacgcag
      421 ggaatttaca agaaattaac aagaaattaa cgcttcatcg gtatagtgga cgtgtgggag
      481 aaaatcacgg aattaaagga ggggtcaaaa tgaccatgat cactgcacac tctggtagtg
      541 acaatacgga ggagaatagc tgggcatttc ttcgcttttg tttggatcgc cagctttgtt
      601 ttgaagtaga tgtgcgtcag caaacagatg gtacgctaat actagcccat gacaggtaag
      661 aagttgtccc agattcggta acccttgagc aggtgtttca agcagtggcc aatgagaagg
      721 caaccatgac cattaactgt gatttaaaag aagagggatt ggaaatggcc gttttttcgc
      781 tggcacaaac ctatcaaatc tggccacaaa ttgttcttag tggctatgta caattatctt
      841 gggctgcgat ttggggaaag catatatttt ataatattga aaatcagttc gaagggaaaa
      901 aacttgggga tattaccgaa gcagatttga tcgatacctt acagaaatta gcctatcaaa
      961 gcgttcaaat cgtcaatatt catgaaggat ggttaactaa gttggcccgt gaagtggggc
     1021 agcagctagg attgacattt tctgtatgga cagtcaatga actagaaacg attgagtacc
     1081 ttgcgcgctg tggtgtttat aatataacct cacgttgtgc atgggcctat ttatggaaga
     1141 aaggagcaca gaataaacta ttttataatc aaaacagagg agttaagaga tttggagatg
     1201 ggatacatga ccatatgggg gctttattct tagcttgatg gatatgtcca gtgaccctgt
     1261 accccgaaat gcacaaattt cgaattattt acagaaaatt aactagaaat ttacattggt
     1321 agttcataat atttcttgat tagaaaaatg gccataaaga aattaggagg aagttaatgt
     1381 tatgttaaag aaaaatcttg ttataggggt tgtgtctgtt ttaagtgtca tctctttggc
     1441 agcttgtggt agtgggaata ccaagacaac aaaaaaatcg gagggtcgtg taacgataga
     1501 gtattggcat gttaatgcgg ataccctagg tggaaaaaca gtgaatgaat tagtagataa
     1561 atacaatcaa tctcaagaca aagtgaaggt tgtagcaaaa tataatcctg atatgtataa
     1621 aggcttgatg caaaatttac aagcttctgt tgcctccaaa gaaataccgg acgtagtaca
     1681 agtgggatgg gcttttaaag attattttgc agaaaatttc aaatatacta atcctcaatc
     1741 tttagtagaa gaagtggatc cggataatgc tgattatttt aaagatcatt ttttagacaa
     1801 tatcatggca ctgtctgaaa aagatggtaa acaagtgggt attccttact ccattagtaa
     1861 tccggtttta tatttaaatc aagatatatt aaaagcagcg ggcttggatg aaaatggtcc
     1921 aaaaacttgg gaagaagttc aaactttttc taagataata aaagaaaaaa ctggaaagta
     1981 tggcgtgtat attcaagaac ctgctgataa ttgggcacaa caagcaattt tagaaagtaa
     2041 tggggcaagt gtaataaaag atggcaaagc tacctttgct tctaaagatg gtatcgctgc
     2101 ttatgaatta tatcaaagga tggtaaaaga ccagacggca ttacacacta cgtgggaaca
     2161 aggtgtgcaa agctttattg atggtaacgt cgcgatggtc tataccacta ttgctcaaag
     2221 ggacaacatt caaaaaaata gtacttttac tgctaaagca gttgagtcac caagttggga
     2281 agggaaaaaa gttaaactac ctgctggtgg cgcaatgtta gctgttacat cacaaacgga
     2341 taagaaaaag gaagcaactt gggatttctt aaaatatctt tatagtgttg aatctttagc
     2401 taaatggaca gaaggtactg gttatttacc accacgtaaa gatgttgcag acgcccaaaa
     2461 tggattaaaa gctttcttag ctgaaaatca gatgatgata ccagcaattg atcagatgga
     2521 taagatggtc agttggagtt catttcctgg agatgctgga ttagaagctg aacaaaaatt
     2581 gttggatatg cgggataaaa ttttatcagg taattccgtc aatgaaacga tgaaagctac
     2641 tcaaaaggaa attaatgagt tactcaaata agcaaagcaa gcagacgaca gcgcctgtaa
     2701 gaaaggaaaa acaaggtcgt ctgcttttga cataaggatg aggaggctgt cggaaatgaa
     2761 gcgctatgta ggttatttgt atctggcgcc agcaattatt ttgttgggcg tgtttgtttt
     2821 ttatccattg atttatacga tctatttgag tttttttgac tggaatatgg tggcccccac
     2881 taaagaattt gttggctttg ccaactatgt aaaggtgctg actgatccag tctttcataa
     2941 agtgttgaag aatactttgt cctataccgt tctatttgtc ttgctgagct gtgtgttccc
     3001 atatattaca tcctttttat taggaatagt tatagaaaaa gggcagaatt tgtataaacc
     3061 attatttttt attccagctg tgatttcttt ggtagtggcg gggatggttt ttacatggat
     3121 attaaatccg gtatcgggac caattgctgt cttgttggga aattttggtt taagactgcc
     3181 tatgtggatg aatttacaag gttgggtaat tgctgtgatt gcaggtatta ctgcttggaa
     3241 agtttttggt tataatttca ttgtcatttt tgcagcaatt gtgggaattg atcaagagat
     3301 gatcgaagca gctaagttgg atcgtgtacc catctggcgc atttttttta caattgtgtt
     3361 gcctatgagt agttcgacag gactatattt attggtgatg accattgttc aaggtctgca
     3421 gtatgtgttt actccgatta agatcatcac tcgtggtgga ccgaataatg gtagtgcaaa
     3481 cgtgatctat tatgcttgtc aaaaggcttt tgaaatgtat aaagtcggag aatcttcagc
     3541 aatttcagtt ttaactttat taatatttgc tgttttctta ggggtaatat ttaaatttgt
     3601 ggaaaagggg gtttactatg aaaattaaac gctggttgcc acatctctta tttatgttca
     3661 tcataatcat tattatctat ccattattgt ttattatggc caattctttt aagccattga
     3721 aagatgccta tcagacgatt actagtttga taccacagcc gtttaccttg gaaaattttt
     3781 gctatttgtt cgaaaatctt ccaattatca aaattacgag aaatactttt tttattgcta
     3841 caattataac ggttgttaag ttaggcgtag ctttttttgc ggcatatagt tttacttatt
     3901 gccgaatcaa agggaagaaa atctggtact ttatcatcat tggtacgatg ttcattcctt
     3961 ttactgttgc gatgattcct aattatttgt tgattgcaaa acttaattta attgatacta
     4021 cttggggtgt gataatacca caatttgctg atgcgatggg cattttttta ttgacgcaaa
     4081 caatgcggga aattccagtg agccttctag atgtggcggc attggataac atcaaacaaa
     4141 caaccgtcat gagtaaaatt gtttttcctc ttactagaca tgcgattact tctaccggta
     4201 tttggttttt tattacctct tggaatgagt atgtttggcc ggtattaatt ttacggacag
     4261 tagataatta tactttgccg ttggcgatgc agacctatat tagttctgaa ggtgggacaa
     4321 attttaccgt agcaatggcg attagtttaa ttacaatgtt tgtaccgtta gtcttgtatg
     4381 caatttttca aaaatatatt atcgggactt ttgtttctgc aggtattaaa taaattggag
     4441 gacaaatcat gggagaaacg cttgaattaa gaaatatcgg aaaaaaatat ggagaacata
     4501 ccattttaaa agaaattgat ttagctgtgg ccgctggaga acgaattgtg ttactgggac
     4561 catctggttc aggaaaatct actttgctga gaatgattgc tggtttggaa aaaattacga
     4621 gtggagagct ttttttaggt acacaactag caaataagct accttgtgga gaacgagaga
     4681 tcgcaatggt ttttcaaaat tatgctttat atcctcacat gactgtaaag gagaatatta
     4741 tttttgcatt aagggcgaat aaaattaaga cagctgacat tcagcaccgt ttggaagaag
     4801 ctttgaagat gttaggttta acgccatacc aagatcgact tcctaaagat ttgtcaggag
     4861 ggcaacggca gcgtgtagca ttggctcgtg cggtggttaa acggagtaat tattttttat
     4921 tagatgaacc gttatcaaat ttggatgtgc gtttacgatt ggatgcacga aaagaattgg
     4981 taaaaattca tcaacgttat aaacaaactt ttgtctacgt cactcatgat caaattgaag
     5041 cgatgacgtt agctgaccgg attgttttat tgaatgaagg tagaattcag atgattgata
     5101 cgccgaaaaa tgtttatacg aaacctaaaa atacttttac tgcaaatttt attggttcgc
     5161 caggcatgaa tattatgcca gttacttatg atcaaggaac attaaaggta gccagtcaat
     5221 ttttaacatt gaatccagct tggcaaaaac aacttcttca ggttagtggc tcattgtatt
     5281 taggcataag accagaacac gtaacctttt caaaagaaac aagcgattta cacggtcaag
     5341 tgaagtatcg tgagctatta ggtcaaagtt atgcgtacac cgttaaagtc gatggtatag
     5401 aaatgattgt ccttaatgaa aaacctactt ggcagattgg tgatgcagtc aatctccgca
     5461 tagaacctga aagaatgcat ttttttgatt atcagacgca agaaaattta ggatatcctg
     5521 attattttat tgaagaaaga gggaatagcc atgcgctttt tgcatctctc tgatatccat
     5581 tttagagaaa actatcaaac taacttgttt actgctgaac tatttccaac aattaaaaat
     5641 ccacagttgg cattggaaaa attgttacaa gaactctcgt taacagtgta cgattttatt
     5701 ctattaacag gtgatttagt acatgaaggg gggatttctg attatcaagt cttacggaaa
     5761 atgttggatc aatatttccc aaccacacct tactattttt gtcgtgggaa tcatgatcgt
     5821 cgcagtgttt tctttgaagg aatgagtgtt gaaagcaatg aaacgggaga gtatatccat
     5881 tgtacgaatc aaaatgggtt acgaattatt tccttggatt ctgctcaaga tacccagcac
     5941 gaaggaaaaa tatcgatagg acagatgatt caattacaag agtggttggg tacacctgct
     6001 gaaaagggaa cgattttatt ggttcatcat ccattgtcgt gggaagatcc atctattgta
     6061 actgaagttc cggaaggttt tgctgaattg attgccgaaa gtgatatttt aggtattttt
     6121 gtaggacata tccatcaagg tagcagtgct gaatttgctg gtaaaaaaca gtttatggct
     6181 gaatctttat catttggtgt agatgaatac ccagactatt ctctttttac caatagaaca
     6241 ggattcacta gtggcagttt aattgacgga aaaattttta cttatcagca ttatttaact
     6301 ccacatcaaa tagaaatagg gcgcatgaaa aaaccaatta ctggtaatca ttaaaaatta
     6361 ttgattaaaa ataaagactt cattcacttt ttagccgtgg tgctcaatgt ctggttccac
     6421 ttgaagaact tggaaaatcg ctatcaataa accactatcc ctccaattac aaaaagggtg
     6481 gagaaagttt tccaagaacc atatcggtct tcgtggttgt gccattcgtt ttttaccaga
     6541 gcataccaca tctttgtgga gttttcagac acgtcctagt taatttggtt cttttaggag
     6601 tcttttcctg ttctgtgtgt cgttgagtgt tcgcttcaat tatcgctgtg gcggattttg
     6661 aaacttttct atctatcaag gccatttagc actatatcct ttccttatat ttagataaat
     6721 acactgcatt tcaaggagtt caaaagtagt tgtattaatt ccatttgaac tctttccgtg
     6781 atataatgtg tttgtcagaa agaggtggag aaaatggcta agttacaaat ttctatggac
     6841 gaaaaattga aaaatgaagc agaaagcatt attgatgaac taggcatgag ccccaaaaca
     6901 gccgtcaccg ttttttataa gcaaattgtt gagcaaggaa aaatcccatt ttctattgaa
     6961 ttgtcggaaa aaagtaaacg aatccataat attcaaagat tagcggaaaa attgcctgtc
     7021 caaaaactag acactgacga aaaaattgaa gaatggttca atgaagatga atactaatga
     7081 tatagcaacc gtctacgttg ctttcacagc tgcagagggc ggtaaacgcc ggcctgtcta
     7141 tattctaaaa aatggactgg acacaatttc gtttctgtca attacttcaa aatatcatac
     7201 taaatccgat aaaataaagc tgaaatacgt tgaaataagg gattggaaac aggcaggact
     7261 aaaaaaagaa tcttggattg acgtaggaac tctaaacgaa ttacccaaaa acaacaaaaa
     7321 ggtcaaatgg aaaaaaattg gggaattaag tccaagcgat ttacagcgac tggaagaacg
     7381 catggaagaa ttataataat actaaaaaga agggctcttt gtcaataagg actgatgggt
     7441 tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcagattt ttggctcttt
     7501 gtcaataagg actgatgagt tgtgcaaaat caaatccggg tcagaatgaa ccacattctg
     7561 gttcagattt tttgttatct tacattgatt aattgattga tcaaaaagat tctaattctc
     7621 atgttctcga atgatttaaa tccgtaggat actcgtttca ttgtctttat gtgggtattc
     7681 ttcgcttcaa tttttccatt ggaataagga tagatcattg cgctggtgat accttcttca
     7741 taggtcagaa ggttttgaag cttctcccga aagccgtcat ctaacgtttc gggaagttct
     7801 gccaataagg agaaaaataa gtcagggtct ttgtctcgaa aggcttcgac taattcatga
     7861 aaaaagggat acgcctcctt tagaggcgca gaaaactcaa gcaatcgatc aatcatcatt
     7921 gcttcagtga gaaatgggta ttttggtgct cggaaacttt tccatgtttt gtactcataa
     7981 tggttgatgc ttgcacgatt ttttagcaga aagcgccagt tctttttcag tttttctgcc
     8041 tgacttttct gtcctgcttt acgaagctca ttcatttcac ggatacgcaa ctcattgagt
     8101 gcctgattca tgtgtttgac aatatgaaac cgatcaatca ctactttcgc atttggcaga
     8161 acacgtttgg taagctggaa gtaagcggcg ttcatgtctg tgactaagaa ttctacttct
     8221 tctggattgg tgcagcctaa gaaatagctt gttaatcgag gtaatttacg cgtaggcaag
     8281 acagcaatta agtttcctgt ttcaccatct gcgcaaataa agctcatttt atcttctatg
     8341 gaagcgtgcg aacgaaattc atcaaccatc aatactctgg ggagaatctt cttagaaggc
     8401 tttggtaaat agcttttaaa ctctttcaat gtacgagtaa cggtagtcaa agatacctga
     8461 caacttttcg caataaaaga caaagatact ttttcagtca gtaaagaagc aattttatat
     8521 cgaacatgat ctgcgattga atggctgagt cggacaaaat aactttgagc cgtccaatgg
     8581 gtacgacagt ttttacaggt atagcgttgc ttttttaggc gcgtaaccaa aggcatatga
     8641 ttgtattgtt caaaacggac aatcgtttcc ttcttcccat ttttcactat aattgctttc
     8701 ccgtttccat ctaccacagt agaaccacaa cttctacaag cacgaggagt aggcgagaga
     8761 acagcatcga cgaccaacgt ctttttcttg gctctttgtc aaataggact gatagagtga
     8821 tataagaaca tctaaggtag aattgatttt gcttctataa tattttgtgt tggtggaaaa
     8881 tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga gactcacttt
     8941 tggtaaaatt tagtcgacca aaacgtcatc ctaccaaagg agtctctcaa tggataatca
     9001 tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag aatggctgat
     9061 tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa ggaaaattgg atgactctcc
     9121 ttcctattgt gaacactgtc gatcggttcg tattatccgc aacggttcct atacgacacg
     9181 cactcagata ttgaaagtca aagaaaagtt aactatttta gaattaaaac gtacccgttt
     9241 tctctgccat gattgtggaa ggaccttttc tgcgaaaacg gacttagttg atgaacatca
     9301 tcaactaaca aaggaactga agcaggcaat tctcatggaa ctgtacgaaa atcaatcacg
     9361 taaactaatc gctaagaagt acttcgtctc ggatgggacg gtgacacgga tcttgcgtga
     9421 agcaacgaag cattaccagt cacgtatgaa cttccttccg acggtgttgt gtatggatga
     9481 gttcaaatca atgaagtctg tttctggttc catgagtttt atttgtgttg atgggacaac
     9541 ccatcagcta tttacgatcc ttgaagatcg cagactttac aaactaactc aatacttcct
     9601 gcactttcct agaaaagccc gtttaaaggt caagtaccta gtgatggaca tgaatgccag
     9661 ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag atcgtaactg accgctttca
     9721 tattgtccaa cacatcaatc gcagcttcaa tcaattacgt gtccagatca tgaatcgttt
     9781 tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
     9841 cttactcaaa gattccacga catgggagcc actcaaacgg cattatcatc gactgtttaa
     9901 acgaccaatt agtcaaacag aaatcgttga tgagctgtta agctacaacg aagaattgcg
     9961 aactgcgtat catttttgtc aattattacg ctattatttc gttaaacgag gtacggaagg
    10021 ctttcaagaa acccttaaaa caatttcatc tacgttacct cagtcgttta aaaagaaatt
    10081 cacgatcttc cattgctacc aatcgggtat ttcaaatgcc tttaaggtgt cccactcaaa
    10141 cggcgtcact gaggggctga ataacaagat caagctgatt aaacgaattg cgtttggtta
    10201 tcgcaacttt tataactttc gggcacggat ctatttacaa caaggtctga tctttgaaaa
    10261 ctaaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt
    10321 gtcttcacca acacgatttg acgaagagcc tatttaaata actacatcca tttattctat
    10381 cttctaaaat agcctttttc atttcagtta aatagttcag cgccattttt gcactcgact
    10441 tgcatagtag ttttttccat attcctcaac ctcagcgggg gttgaagaca taaggaaaat
    10501 cctatgcgtt atgctcaact ggatctgatt aaatattcta atgtattttt ctctaccatc
    10561 attaaatggt taatagccaa aaatcaccac ttttcaatgg ctccttgttg tataagtata
    10621 actagaagat gaacacgtct gagaaattat agaagctttt tacggtaatt actggggctg
    10681 tgtccagtag tttttttgaa gagaacagaa aaatagctca cgtgatctat tcctactttt
    10741 tgactaattt catcaataga taagaatgtg tctttcagta gagcacttgc tcttttgatt
    10801 cgaaagtcta gcaagtaatc atgaaaggaa taacctgttt cttttttgaa ggcccgtcct
    10861 aaataagcgg gatggtaatg gaattgtgta gctacatctt tgagcgtaag cttttcacta
    10921 taattgtttt gaacgtaatc aatcacttgt tgaattgact cattaaaaga atcatcagtt
    10981 agagagtttt gattagctgc tgatttacgc attttttctt cttgtactaa cttaactaaa
    11041 taaggaaaat caatgggttt cgttatgtaa cttttaatag ataaatcaat cccctttttt
    11101 gcatactgaa aattttggtg ggcactaatg acaatactag taaagtagcg attggatgtt
    11161 tgttgcaaag attcgattaa ttcgagtcca gaaattttcc ccatcgtaat atctgtgata
    11221 agtaaatcaa agcgctggtt ttgcaggttt ttaaatgctt cgtctccgtc tgtgtagcag
    11281 tgatcgataa tgatgtcggg gactttcttc aaaagataat gccttatgcc ttctacaatt
    11341 gtacaatcat cgtcaacaat tgcaattctc aacatgcata ttcccccctt tttgtatttt
    11401 gatagtaatc gttgttccga cgtgtagttg gctttctata acaaaacgtg tatctccagg
    11461 atagagatgc tttatacgtc gaaaagaatt tgagatgccg atattctcac tataaggaat
    11521 atcatcatta agtttttgtt gaatatgctc cagcttgtct tggtccattc ctttcccatt
    11581 ttcaacgatt tttaacacat aatggttggt tgtttcagtt gcatagacag tcaaacgatt
    11641 ttcctttttt gatgaatcga agccgtgttt aatataattt tcaacaaatg gctgtaaaga
    11701 aagttttggt aacattaatt gatcaatatg atcaatcagc tgatagtcaa agttatgtcg
    11761 ataacgacgt tgatgtagtt gcaaggagag gtcgatatta attaattcct ctttcaaatc
    11821 aattatgtcg ggctgatatg tattattgcg atacaacttg ctcaataaat atatactgtc
    11881 gctgatttct tcgtcatcat tcaatatggc agtcattcga ataaattcca aattattaaa
    11941 tagaaaatga ggctcgattt gatattgaag tgctgcatac tttgcttctt gcttcaacca
    12001 attattttca taaagtgttt tttgatatgt agcattatct aaataaaact gattgatatg
    12061 catagcgatt tctttcaaat caccgacttt gttttcgata ttgattgact tgtagtctcc
    12121 tgtagatgaa tgatttaagg aagtaatgat ttcctgatac tgctggatat agttacgaaa
    12181 acttctattt aataattgat gaatagcaag aataaacaaa ctcattaata agaaaatcat
    12241 gattgccaga cctaataagg tttgtgacga ataatttgga ttttcatata cttgtaatgt
    12301 gtggttaccg atagctatct gttgcgtttt tttccattta gaaggcgaga tgtcttcaga
    12361 taaattgacg gaatcaatac cattagacaa agaataattg taatgtgttg gaaagagact
    12421 cttttttagc gcgttgatcg aaacgtctag aatcaatgta gcatcgggca tgtcctcact
    12481 aatgaaactt aatgaacgaa taaaagaaat tttttcgttt tttgtaggtg cgtaggcact
    12541 taagtatttt tcgtaatcta atgagataga tatattttta acttcatcat cttgcacaag
    12601 tttttttaag ttatctacta aataggtagg ggagcgatcc tttgacaaaa tatcttccat
    12661 gtattcagca ggtgatgaga ggtagtagtc attaaggttt tcttggaaat cataattttg
    12721 atagatctct gtcacgatac cggagagaat acgattatat gtttccatat agtgggtgat
    12781 ttgctgaatg ttttgcaggt tttgattttt ttctttttca atcacattga tataggaaaa
    12841 gaacaatgtg aaacaggcaa gtaaagtaat aaaaaaaatc aatatgtatg taaaacgcat
    12901 aataactttt gataaatcgt ttttcattca aagtcctcat tttgataatc tctaatttta
    12961 tgaatcatta tagcacatat tacaagtgat gtaaacgctt tgcaaacagg gctacttatg
    13021 attgtgaata cagtttccgt ttaaaaacta ttctattgta gaggtttaat aaactaaaaa
    13081 taatgtataa aaaattaatg taagcgatta accaagtggg tataatcaag gaaacgctga
    13141 gagaggagag acaaaatgaa gctaaagaaa gggttattag gaaatctaaa aaaaaaccga
    13201 gcattgttta ttatgctttt accaggtgcg ttatggttat ttatattttg ctatatacca
    13261 atgtttggag ttgtaatcgc ttttaaagat ttcagaattt catcaagggg attctttaca
    13321 agtttgttca acagtccttg ggttggctgg gaaaatttca aatatctctt tgcttcgcaa
    13381 gatgcttgga tcattactcg caatacggtt gtttataacg ttgtaggtat tttgctaagt
    13441 actttttgta cagtgtcatt agcaatcatt ctgagccaaa tggcgaacaa acggttattg
    13501 aaaaagatgc agacatttat gatttttcct aattttttat catgggtagt catcagttac
    13561 tttgtttacg catttttgag tacggataaa ggattaatta atattttact aagcaatttt
    13621 ggtgtggaac ccataaattg gtatagtgaa ccgaagtact ggccttttat tattgttttt
    13681 acgagtttat ggaaaggcgt aggctatgga tccatcattt attacgcgtc aattatgggg
    13741 atcgatacta gttattatga agctgcttta gttgatggtg ccagtaaatg gcaacagata
    13801 aggaatgtag tcttgccaca tttaagatcg attattgtca ttatgttgat tttgtctgtt
    13861 ggtagtattt ttcgagctga ttttggattg ttttatcaat tgccaagaaa ttcgggggct
    13921 ctttatgatg tgacgaatgt attagatact tatgtctacc gtggattaat gaatagcgga
    13981 gatatcggaa tgtctacggc agcgggatta tatcagtcat cagttggctt tgtcttgatt
    14041 ttgattacta ataagatagt caacaagtta gatcctagtt cagcactatt ttaggaggaa
    14101 tcaaaatgat atcaaaatta aaacggcaga tgtacttttc gctgccagtc gaacggtttt
    14161 tcacaatatt actaatctta tttactctta gctgtatttt tccctttatt ttcgtaatcg
    14221 tcatttcatt aacagatgaa aaagcattac aactcaacgg ttacgaatta ttgccggctc
    14281 aatggagcac tgatgcatat caatacttaa tacaagatgg cggacagtta cttcggtcat
    14341 taggcgttac aattatgatt acagtgattg gaacgttgat tactgttttt atgacgggaa
    14401 cctatgctta tgttttatca agagcctctt tcccttaccg gaagttcttt actttttatc
    14461 ttttttttac catgttattt gcgggaggga tggttccttc ttacttagtg atgacgaaga
    14521 tgcttggctt aaaaaataca atttgggctt tgattctacc tcttgctttt agcccataca
    14581 atgtcattat tttaaggaca ttctttaaga agtcgattcc agaatcgatt atcgaatccg
    14641 ctaaaatgga tggctgtagt gagtttcgag tttttttcca aatcgttttg cctttagcga
    14701 tcccaggggt tgcaacaata ggtttatttt caagcttagg ttattggaat gactggttca
    14761 acgcattgct gtatattgac accaacaaat tgattccatt gcaatatttg ttaatgaaga
    14821 ttcaaaattc aatggaattc ttagcaaata ataatgacat tacattagcg cagcaacaag
    14881 caattcaaaa tagccttcct caagaatcaa cacgaatggc aatggtcgta gtagctactt
    14941 taccgattgc aattgtttat ccgttcttcc aaaaatactt tgttcaagga ttgacgatcg
    15001 gtggagtgaa agaataaaaa agaataggag atgttaaaag atgaaaaaga acatgcagaa
    15061 atatttgatt ggtggtgctt ctgtactaac ccttggccta ttaggtgctt gtggtagcag
    15121 taatggcgaa gcaaataatg aaacaggaac cacaaaagtt acttggtatc aggttggaga
    15181 agaacccaaa gattttgatg aggtcatgac aaaagtcaac gaaaaattaa aggaagaaat
    15241 caatgtagag ttggatatgc ggctgattcc ggacggggat tatcagcaaa agcttggtgt
    15301 gatgatcaat tcaggagaag aatatgatat ttgtttcgtc aatggcacag attatgtgaa
    15361 ttatgggaac aaaggtgctt tcattagtct caacgatcat ttggataaag agttgaaaga
    15421 ttatgcagct gaattgaatg aaggatttat tgaaggtggg gcaattaatg gcgaaactta
    15481 tggattgcca gtcaataacg atattgccac aacggaatat tggaccttta ataaagattt
    15541 ggtagatcaa taccaattgg atgtagagaa tgttgaaggt gtagaaacat tggctccagt
    15601 attagaaaag tttgcagaaa aaaatactga tacgaatatt acgccaatcg gtatgacccg
    15661 taccagtgtc tgtattcctt atgacaatat tatcggtggt ggtgtaccgc tagcggttgc
    15721 atttgaaggc gatacacaaa cagttgttaa tacttatgag actccagaaa tgatgactta
    15781 tttgaaggct atgcatgagt tttataaaaa aggatatatt ttgaaagatg cagccactgt
    15841 tacgacagat ccatttcgaa ttgatagtaa aaactggttt ttaggtcgta actcaagtgg
    15901 ttatcaacca ttagcacaaa aaaaattaga agctctagcg ggacatggat taattttaaa
    15961 accagctgga aaagctttta aaacgactgg atttgcaaga gtggcaatgc atgcggtatc
    16021 tgcttcaagt aaacatccgg aagcagcctt gaaattgttg aattatgtga ataccaatca
    16081 agaagtagcg gatttaatgg tttatggaat tgaaggaaaa cactatgatc gcaatgaaga
    16141 tggttcgatt actttaacaa actctgatga ttattatcaa ggcgcttggg cattggtaaa
    16201 tacttatcta gtttcaaaaa cttcaccgat tacagaagaa agtaaaaaac tagaagaaga
    16261 aagtaaaaag tttcaaaatg aagcaccgat gtctcctatt ttaggtttct cttttgacgc
    16321 ttctgatttt cgaacagaaa ttgcaagtgt acaaaatatc gtgtcacaat atgaagtact
    16381 tttaagctcg ggaacagttg accctgaaca atatgtacca gagttgttag aaaaactaga
    16441 aaaagctgga ttggataaaa ttcaggcatc tgctcaagaa caatacgatg aatggcgaaa
    16501 atctaacaaa taatttttta ctaaatgaaa agaatagact aagcagttag tggggggatt
    16561 caaatgaacg aaaacaaatg gaccaaagga tttaattggg tttttcaatt gttgttacta
    16621 aactttactt attttatact aatttgttta ggatttggaa ttttaggttt tttccctgct
    16681 acgatagctt tgattgctgg cgttgtgaat cgaaaaaaat atggcttgca agagtctgtg
    16741 aagttcatga gtaaaatcta taagaaaaat tttgttagat ataatctata ttgctggtca
    16801 gcaactttgt tgttggccgg caatattact acgatctggc tttgttggca gcaaagcgca
    16861 ccattgtttt atttattaag tacattatta tcggtaatta cactgttcct agtctttgca
    16921 atgttgaatt gggtcgtgat caaaagtctt gggttgtgga tgatgggaga ccgactgatt
    16981 gactatttgc ttttcacagc aacgatgttg ccaacggtag tcatgcaagg cttggtgctt
    17041 ggaatttatt tccgaatatt gatgattttg cctgtgattt ttctgtttgg cggaacagct
    17101 gtaacggtaa tattgattag taaattaact gaaagagcgt tagcaaagtt gccttgtcag
    17161 atacaatcgc actaattttt tcacaaaaag gagtatttct ttctatgaag ttaactgata
    17221 aacaagtagc taatatagcc tttatccagc aacttgagat tccattgaca caaaaagatg
    17281 aagaacaaat agacttgatt tttactgaca caaaggctaa tgtgcgacaa atcaagatta
    17341 ctaaaaatga gcagcaacga atttgcttgt cttattcaca tgaagacttg ctgtatcgag
    17401 gattgtttat ctgccttcca cgttttttag ctcaaggagg agagatgcca tacattgaag
    17461 aaacctcgat cattgagacc agttattcat tagatgcttc tcgtaatggc gtgatgaata
    17521 aagagactct aaaaaaattc atttcttact tagcgttgat ggggtacaac acattgtata
    17581 tttacacaga agatacctat gaagtagaag accttccata ttttggttac cttcgcggag
    17641 cgtatacaaa agaagaagtg aatgaaattg ttgaatttgc ttctggtttt ggcatagaag
    17701 tagttccatc gatccagacc cttgctcact tgacccaatt tttgaagtgg tatgctcaac
    17761 aagaactgat ggatgatcaa aatactctac tgatcggtga agaaaaggtt tatgcggcaa
    17821 tcgaaaagat gattattgcc tgcaaagaaa tgtatcagac agagcgtatt catttgggaa
    17881 tggatgaagc ctatcaagca ggtctgggac gatatttaag cctgtatggg tattgtgatc
    17941 ggacaacttt aatgattcaa catatggacc gtgtgttatc aattgttgat aaatataaga
    18001 tgaaaccaat gatttggagt gatttcattt ataagctatt agatgaaacc ggtaaagata
    18061 ggtattacaa tcctgatttt aaactcaaag cattagcagc taaaggatta ccagcaggat
    18121 taacctatgt tcattgggac tatggttcag atgaagtttc ctcctatgag aaagtaattg
    18181 ataaccattt gaaattttgt gacattgatc attacgtttt agctagtggt gcccatatct
    18241 gggggagact agccccgaat catgggaaaa cagaagcaat tgtttctgca gctgtaaaag
    18301 cttctaaaaa taaaggtgta cgttctgtga tgttaactac ttggggagat gaagggcagg
    18361 aaacagagca ttggcattcc ttaattgggg cattaaaatt agcagattat gtctattctg
    18421 aagaagagtg gaatgatttt gaggaaatct acgatcgttt attaggaaca ggtagttatc
    18481 aattaatgtc cgaattacga tatttcgatg aagtggatac cattacaaat aacaacttac
    18541 aaatggcttc cgtgagtaaa ctgctacttt ggcaagatcc gttagttggt atatatgact
    18601 ttcatgtagc cgctcataac aaaattgcca ctagaacaat tggtacttat tatcatgagt
    18661 tagctcagaa attagatatg aataaaaatt ggaatccaaa acaaccaatt ttaacaacta
    18721 ttcgtcaacg atatatactt ttagctgaaa ttttagcaca aaaggcagaa ctaggcatct
    18781 atttacaaca ctgctgtctg gtagaagacc ttgcaggatt aatggaatta gcgaatcggt
    18841 gtgaagcact agccaatcga gtaaatgaat tggcatctat tcatgaaata atttggtatc
    18901 aaacatacaa aacctatggt tgggaagtat tagaacaacg atatgctgca ttagctcatc
    18961 gtctgaaaac cgttgcgcgt cgtattaaaa atcatcttca ggggcaagat gccctagagg
    19021 agcttttgga agaacgatta ccgttttgtc aatatcaagt gccgcttgaa atttctggct
    19081 ttaattatcg gcaaagctcg gtgtcaggct acaattaacg aaggaatctt cccgttgaac
    19141 ggaccacttg ctcatctaat gggaaagtgg ttcgttttct attcataagt agcttaccac
    19201 taactctttt attaagaaga gaaaaaaagt aggaagtgag aaaatatgtt atcggactat
    19261 tatcaaaatc tgtcagttat aagtgtcaat acagaaccat tacgtaatta ttatattcct
    19321 tatgcaagta aagaagaagc aaaacaagag ttagaaaggg aaaaaacgag ccagtttatc
    19381 gatctcaatg gtacatggga ttttcagtat tatccttctg tccaacagtt accggaaaat
    19441 ttttttcaag aggcctttac tgaaagctat gatagaattt ctgttcctgg agtttggcaa
    19501 acagagggat ttgacttacc tcagtatatc aatgtatctt atccaattcc ttttgatccg
    19561 ccttatgtac ctaatgatac cccttgtggt gtatatcggc gtcagtttca aattgagcaa
    19621 acagatgcac gcttttactt aaatctagaa ggagcagata gtaattgttt tgtttggata
    19681 aatagaaact ttgtaggcta tgaccaagtg agtcatagta catctgaatt tgatgtgacc
    19741 aagtatttac aggttggggt taatgaaatt caaattctag tgatgaagtg gtgtgatggt
    19801 acctactttg aagatcagga taagtggcga atgagcggtc tatttcgtcc agtgtatctt
    19861 cttcaacgac caactatgca tctgacaagt tatcaactac ggacgttgac tgatcctaaa
    19921 aaaggaagcg gaacattaat ggtttctttt caacgattta ctcctattga agtatcgtta
    19981 cctatttctt acgaattatt ccatcctgat ggacgtctca tagcgaaagg ggagtgttac
    20041 tcggaacaac aaataaattt ggatgttgaa gattgccttt tttggacagc agaaacgcct
    20101 aaattatata cattactttt aacatgtaat ggtgaatatt ttaaagaaaa agttggcttt
    20161 cgagcactga cgatcaagaa tcgtaagtta ttgctgaacg gaacacctat taaattatat
    20221 ggagtcaact atcatgacgt gaatcagcaa ggcacgccag tgatgacccg agagcagtta
    20281 cgtgcagatt tgttattaat gaaaaaatta aatgtgaata ctgtacgaac ggcacattac
    20341 cctaaatcac cagattttta tgaattggcg gatgagattg gtctgtatgt aatcaatgaa
    20401 gcagatttgg agtgtcatgg tgtggttaat ctatatgata ctgaagcgaa ttatcaacta
    20461 attgctaatg atccgacatt cgaaaagccg attatggacc gagtgatccg ttctattctt
    20521 ccttatcaaa atcgaagctc tatcattatg tggtctatgg ggaatgaatc tggttatgga
    20581 gtaaattttg aaaaagctca agctttagca cggaaattgg atgattcacg gttgattcat
    20641 aatgaacgct cgattgaacc agttattgac caagtgaatg attttagtaa cttggatgtt
    20701 attagtcaaa tgtatccccc actggaagat gttcatcgtc attgtcaaag aagtgatact
    20761 gaaaaacctt tttttatgtg tgaatatagc cacgctatgg gcaacggtcc tggagatctt
    20821 aaaacgtact tcgatttgat tcaacagtat gattcctttc ttggcggttg tgtctgggag
    20881 tggactgatc atgcaatcaa tagaggaact ttagaaacgc catcttattt atacggtggt
    20941 gattttggtg aaaaatatca cgacggcaat ttttgcgtag atggtttatt gaccccagat
    21001 cggcaattaa cagtaggtgc attggagtac cgtaatattc attcgcctat tcaactgatt
    21061 gctgtagatg aggagctgca agaagtaacc ttgagcaata taactgcttt tacagccggt
    21121 tcagcattta acctttctct tgaatggtat atagatggaa aaaaaacagt ttatgaagta
    21181 atgaatcctt tcatacttga gcctggcaaa agttgttcat tgcccatcac agtacctaca
    21241 acgattcatg gtgctcatac ggttgctgtt tcgctatctg tttataaaaa tgatgagctt
    21301 tatggacagc aacagctctt tcttaaaaaa gaaaaaatga gattgccaag aactgctcat
    21361 cataatgatg caccacaaat ttcagtaaaa ggcacagagc tgataatcaa aggagaatcc
    21421 tttatttata tatatcaatt agcaaaagct gcgttcactt ctgtaaagat caatcaaaag
    21481 gaacttttgg aacgtcctac tcaattttca atttggcgtg caccaacgga taatgatcgt
    21541 aacttagtaa acaaatggaa agcagctgga tatgctgaaa tgtctttaga aatcagaaaa
    21601 aaagactgga aattcgtaga tgatcatctc ctgatcgaaa cgtcttatcg aggagttatt
    21661 ccaggattac agccaatttt tacaatgcac atacagttga aaataaatgg tgcaggcgta
    21721 atcaaccaaa aagtatcagt ggagaaaaat ccgatttttc cagatttccc tcgctttggg
    21781 atggactggc cgttgaatga tactttttcg aaggtttctt attgtggcta tgggccgtat
    21841 gaaagttatc ctgataagca ctatgctgcg atgtttggta tttatgagat ggagattgag
    21901 gaatttgatc agccatatat caaaccacaa gaatatggta accgctggca atgtgattgg
    21961 gtatcaatca aaaatcatca aggatctacc atgacggttt atggaaactc taatttttct
    22021 gctctgcctt atagtcaagc tcaattaacc gaaaagtcac accattatga attaataaaa
    22081 gaaaatagaa cttggttcaa tttaagttat aaacaaaatg gtatcggctc acacagttgc
    22141 ggtccttatt tagaagatcg tttccgtttt atggaagata cttttgactg gaatatagtg
    22201 attactttca aaggagaaaa tagatgaaag aaacaaagat caatcaagtc aaaaaaaaat
    22261 tatctcgtta tcaagaagtt gtttatcgaa accattgctt aaaatggtca aaaaataagc
    22321 tgaatgttcg ttttaatgga ttattttatc gtttcgaaca gttagaagaa gtctggaaac
    22381 cggtaacgaa tttccaatca tgtcctgtac agctcataaa tgatccaaaa gaaacatacg
    22441 atttatgtat aacacacctt tataaccagc atttgaaaaa tgacggattt tatttaaagg
    22501 ttactaatga caatcaactt acgattgaat cagcaaacct tcgagggttt caatatgcta
    22561 tggaagcctt attagagagt tttttttcgg taggagatca attattatta ccggaattga
    22621 ttctgaaaca tgaaccatca gtgaaaattc gaggaatcat tgaagggttt tatggtatcc
    22681 cctggacaca tgaaatgcgc ctctctcttt tttcatatat gaaagataac caattgaata
    22741 cttttatgta tgcccctaaa gatgatgaat tattacgtaa aaaatggcgt gaactctacg
    22801 atgcgcaaga attagacaaa tttaaggagc ttttatctgc tgctgaggct agcaatatcg
    22861 atttttggta cttaattagt cctggaaatg atattgatat tacgtgtgaa gaagacattc
    22921 aagttcttct gaaaaagtta gaacaactga ttgagcttgg cgtatttcaa tttggtctat
    22981 taatggatga tattgactat caattaaaag gtgctgctaa acgccgtttt cgagagccag
    23041 cattcgctca tgcctattta gtgaatcgag tagaggaata tcttagcact cggttgtcca
    23101 atcatgaatt agtcatttgt cctacagaat atgataatcg tcatggttct cgctatttag
    23161 caacgttaag tcaagaaata ccagaacaat taccgttctt ttggactggc ccaagcacgt
    23221 tagcggcgtc aatttctaca gaagagctgc aggaaatggc ttctgtctat caacggccga
    23281 tgctgatttg ggataatatt ccggtaaatg attatttaga ggataaagaa ctattgttca
    23341 tgagtccata tgagaatcga acaccgaatc tttctaagga gagataccag gttacaggag
    23401 tagtttcgaa tccaatggct caattagaag cttcaaagtt tacgatcaat agtatggcta
    23461 attacttatg gaactgtgag cgttttgatc ctttggaaac ttggacgtca gtcgttgaaa
    23521 aagtagttgg aagtaaacta caaccagctt atcttacctt aaccaatgct tttccaaatc
    23581 attacacaag cagtctgctt tcagatgaag aattactgct agccaaagac ccagaatggg
    23641 tcacaaaaga aatggctgct ttagtccaag cagtaaaatg gatccagcaa gaacaacata
    23701 catctagatt gagaacagaa ttggagcctt ggttacaagc aatcacagaa agttggactt
    23761 tctggcaaga atggcaatta aaaaaagatc caaaagaagc agaagaatgg ttggctaaga
    23821 aaaaaacaca ccgcatagga cgtgatttag taactgccca tttagagcaa attatcaaaa
    23881 gttagatatc ttaaaaaata aaaagcaaga aaaaggagaa caaaatggtc aagagattaa
    23941 ttagtgcaaa ctacagtgat gtcaagaaaa tgacgtcaaa agagttgaag caatccatca
    24001 aagcgagtga aggacgggtg attgtttgtg aaacggttgc taatactctt ccacaatctg
    24061 gagaaattac gaatgcagaa gttgcgtcag catttggtgc agatttgatt cttttaaatg
    24121 gtttcgattg ttatcgtcct gcagtttttg gactaccaaa tgtgacgatt gagaaggttt
    24181 ttgatctaca gcaaacgatt gaaaacccga ttcctaaatt aaaagaacta gtcggtcgtc
    24241 ctgtgggaat taatttagaa cccattgccc ctaaaacaaa tatgatgagt gagaaagtag
    24301 aaatcagcca aggaagagca agctctgttg aaacgataca aaaagcagaa gaactaggag
    24361 tcgattttat ttgctttacc ggtaatccag gcactggagt aaccaattta gaaattacta
    24421 aatccgtaaa aatggcaaaa aagtattttt cgggattgat tattgctggg aaaatgcata
    24481 gcgccggctc agaagagcca gttgtgaata aagaggcgat tatttcttat gcggaagcag
    24541 gcgcagatat tttactagtt ccttcagtag ggtctatccc aggcttttta gagcaagacc
    24601 taattgaagc ggttcggttg gcaaaaaaat atgatttgtt aacgatgtct gcaatcggta
    24661 ctagtcagga aagtgcccga ccagaaacat tgcggcaatt agccattatg aataaaagtt
    24721 gtggtgttga tttacaacat attggagatg ctggatacgg gggattggca ccagcagaaa
    24781 atattttcga aacctcggtt gcaattagag gaatgcgaca taccatcaac tgcgttagcc
    24841 gctcgatcaa tcgctaacaa aattaaataa aaaatggtta aattgattat ttaagtggga
    24901 acatacttga gatatcagct aacttctgtc actttaaaaa agggactgtg acagaagtga
    24961 aaatagacaa aaaatatccg aacaagagac taggatagca gaaaaactat cgttttgcaa
    25021 cgaggaccaa ctggaccgta ggagcaattc tcttattgtt cggatatttc gcttgttcaa
    25081 ggacttatgt ctgtagtgcc cttttttggt aggaaaaatg gtagggaacc atatcatttt
    25141 taatgaaaat caagcatttc aaaagt
//
LOCUS       DACDKL010000022        23963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDKL010000022 DACDKL010000000
VERSION     DACDKL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.022"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            96..371
                     /locus_tag="IV329_000710"
     CDS             96..371
                     /locus_tag="IV329_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8939914.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            377..1144
                     /locus_tag="IV329_000711"
     CDS             377..1144
                     /locus_tag="IV329_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8939915.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1312..3141
                     /gene="typA"
                     /locus_tag="IV329_000712"
     CDS             1312..3141
                     /gene="typA"
                     /locus_tag="IV329_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8939916.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3402..5174
                     /gene="recQ"
                     /locus_tag="IV329_000713"
     CDS             3402..5174
                     /gene="recQ"
                     /locus_tag="IV329_000713"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8939917.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5288..5590
                     /locus_tag="IV329_000714"
     CDS             5288..5590
                     /locus_tag="IV329_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8939918.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5593..6756
                     /locus_tag="IV329_000715"
     CDS             5593..6756
                     /locus_tag="IV329_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8939919.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6782..10210
                     /locus_tag="IV329_000716"
     CDS             6782..10210
                     /locus_tag="IV329_000716"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8939920.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10231..11355
                     /locus_tag="IV329_000717"
     CDS             10231..11355
                     /locus_tag="IV329_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939921.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11345..11782
                     /locus_tag="IV329_000718"
     CDS             11345..11782
                     /locus_tag="IV329_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8939922.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11766..12104
                     /locus_tag="IV329_000719"
     CDS             11766..12104
                     /locus_tag="IV329_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8939923.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12223..12783
                     /gene="rsmD"
                     /locus_tag="IV329_000720"
     CDS             12223..12783
                     /gene="rsmD"
                     /locus_tag="IV329_000720"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8939924.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12780..13271
                     /gene="coaD"
                     /locus_tag="IV329_000721"
     CDS             12780..13271
                     /gene="coaD"
                     /locus_tag="IV329_000721"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8939925.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13261..14304
                     /locus_tag="IV329_000722"
     CDS             13261..14304
                     /locus_tag="IV329_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8939926.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14463..15191
                     /locus_tag="IV329_000723"
     CDS             14463..15191
                     /locus_tag="IV329_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8939927.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15307..15552
                     /locus_tag="IV329_000724"
     CDS             15307..15552
                     /locus_tag="IV329_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8939928.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15549..15767
                     /locus_tag="IV329_000725"
     CDS             15549..15767
                     /locus_tag="IV329_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8939929.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15903..16442
                     /locus_tag="IV329_000726"
     CDS             15903..16442
                     /locus_tag="IV329_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8939930.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16435..18708
                     /locus_tag="IV329_000727"
     CDS             16435..18708
                     /locus_tag="IV329_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8939931.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18746..19792
                     /gene="holA"
                     /locus_tag="IV329_000728"
     CDS             18746..19792
                     /gene="holA"
                     /locus_tag="IV329_000728"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8939932.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19881..20135)
                     /locus_tag="IV329_000729"
     CDS             complement(19881..20135)
                     /locus_tag="IV329_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8939933.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20535..20804)
                     /locus_tag="IV329_000730"
     CDS             complement(20535..20804)
                     /locus_tag="IV329_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939934.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21274..21729)
                     /locus_tag="IV329_000731"
     CDS             complement(21274..21729)
                     /locus_tag="IV329_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8939935.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21867..23795)
                     /locus_tag="IV329_000732"
     CDS             complement(21867..23795)
                     /locus_tag="IV329_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8939936.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         7983 a         4115 c         4987 g         6878 t
ORIGIN      
        1 atattattat gtaaacttaa aaatatgaca acttcttggt ttattaccac ttcctatgct
       61 acactgaaga ggaatatgaa ataggagagt tgaagatgca agaatatcag tatccacttg
      121 atttagattg gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac
      181 gtgcgaatga aaaagggata aacaatgaag cttttttgaa gacttatcag cagtttaaaa
      241 ctgttgtcaa atccattggt gaggaaaaac gattgggacg ggaatttgaa aaagcttccg
      301 gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa
      361 tgaacgggtg agataaatgg aacagatgat tgatcatatc aagtcctggc ttgaagaagc
      421 tggtgagaaa atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac
      481 ggatctagta acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa aaatccattc
      541 ctttgatcca gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat tatcggatct
      601 cgatggaaga gtctttatca tcgatcctat tgatggcacc ttgaattttg tgattgaagg
      661 ggaaaatttt tgtattatgc tcgctgttta tgaatccggc gttggaaagc ttggtttcat
      721 ttatgacgtg atgagaaatg aacttttttg gggtggaaaa ggaataggcg tgtataaaaa
      781 tgatgagcca ttgcctagac cagcaaataa atccttatcg gaaggcttac ttggtgtgaa
      841 tagttatctt ttcggtcata ataaatatgg aatccgagaa attgcggaaa gcagcatggg
      901 gacacgtatg tacggatgcg ctggtcttga actagtggca atcttgaaag gaaaccacat
      961 tgcttatatt tcaaacctta gcccttggga ttatgcagcg ggaaatgtct tattggaaga
     1021 gtttggtatg gtaacaagcg gtctttttgg tactcctttg acatttgaaa aaagggaata
     1081 ctatttagca gggacttctt gtgcatatgc agaaatcgca gaccttttga aaaaagaagg
     1141 ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag attaattttc
     1201 tcgaaaaaat gaaaacattt tactatattt catgaaaaaa gtctgctata attagcagtc
     1261 gagtaaaatt tgccctgtca taaagaacca ataaaaaagg agttagttac attgaattat
     1321 agaaacgata tccgtaacgt cgcaattatt gcccacgttg accacggaaa aacaacatta
     1381 gttgatgaac tattaaaaca atcagataca ttagacgcac atacgcaatt gcaagaacgt
     1441 gcgatggact caaatgcgct agaaaaagaa cgtggtatca cgattttagc caaaaacaca
     1501 gctgtagact ataaaggtat tcgcgtaaac atcatggata ctccaggaca cgcggacttc
     1561 ggtggcgaag tagaacggat catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg
     1621 tatgaaggaa caatgcctca aacacgtttt gtattgaaaa aagcactgga acaacatatc
     1681 acaccaatcg ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga gcatgtcgta
     1741 gatgaagttc ttgaactatt catcgaatta ggagcagatg atgatcagtt agactttcct
     1801 gtgatttatg catctgcact aaatggtacg tcaagcttgt cagatgatcc agcagatcaa
     1861 gaaccaacca tggcacctat ttttgataca attattgaaa aaatccctgc tcctgtcgat
     1921 aacagcgacg aaccattaca attccaagtt tcattacttg actacaacga ttatgtaggt
     1981 cgtatcggaa tcggacgtgt atttagagga acaatcaaag taggagacca agtagcatta
     2041 attaaattag acggaacagt taagaaattc cgtgtaacga aattatttgg tttctttgga
     2101 ctaaaacgtc tagagattca agaagcaaaa gctggagact tgattgctgt ctcaggtatg
     2161 gaagatatct ttgtcggtga aacagttact cctgtggatc atcaagatgc gttacctatc
     2221 cttcacattg acgaaccaac attgcaaatg actttcttag ttaataattc accatttgcc
     2281 ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag aacgtttgat ggcagaactt
     2341 caaacagatg tatccttacg agttgaacca acaaactctc cagatgcatg gacagtttct
     2401 ggtcgtggag aattgcattt gtctatcttg attgaaaaca tgcgccgtga gggatacgaa
     2461 ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg
     2521 tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg gtagtgttat cgagtccttg
     2581 agcttacgta aaggtgaaat gcaagatatg gtccatacag gaaacggaca aatccgtttg
     2641 acattcttaa cacctgcccg cggtttgatt ggttattcaa ctgaattcct ttcaatgaca
     2701 cgtggttacg gaatcatgaa ccatactttt gaccaatatc tgccaatgct tcctggtcaa
     2761 attggcggac gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc aacgacttat
     2821 tctatcatga gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac agaagtatat
     2881 gaaggaatga ttatcgggga aaacagccgt gataatgatt tgactgtcaa catcacgaaa
     2941 gcaaaacaaa tgacaaacgt acggtctgct actaaagacc aaacatctgt catcaaaaaa
     3001 ccaaaacaat tgacgttgga agaatctttg gaattcttga atgatgatga atattgcgaa
     3061 gtaacaccag aaagcattcg cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa
     3121 gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag agaaaccagc ttgttataaa
     3181 aaatagctgg tttctttttt tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg
     3241 gacataaatt cagaaaatac gccgaaagac gtttaaaaga cttttaaaaa acttatttct
     3301 agagagtttt tgtttcataa taagataatg tttcacgaaa ggagatcaac ttgtccttcg
     3361 aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac
     3421 aatactttgg ttatgatgag tttagacctg gtcaaaaaga aattatccag aaagtcattg
     3481 atcaggaaaa tgtattagga atcatgccta ccggaagtgg gaaatcaatc tgttaccagc
     3541 ttccagcttt actattagat gggcttactg tggttgtgtc ccctttaatc tctttgatga
     3601 aggatcaggt ggacgcggcg aatcaattag gtatacccgc cacttttatc aatagctcgt
     3661 tagatggtta cgaaacagct agacgcttcc aagaaatcga tagacagcag taccgtttac
     3721 tttatatcgc tcctgaacgt tttatcatgc cggattttat ccaagcaatg aagcgatgga
     3781 acgtccgtat gatcgctatt gacgaggcac attgtatctc tcagtggggg catgatttcc
     3841 gcccaagtta tttacagatg gcgaaccagc tagatcaact gccaaatcgt cctgtaattg
     3901 ttgctttgac agctacagcg actgtgcaag tagctgctga cataaaaaga cttttaaaaa
     3961 ttccagaaaa caaccatatt cagactggtt ttgaacgaga aaatctacga tttcaagtaa
     4021 ttaaagatca aaaaaaagaa caatatctaa tcgaatattt aaagatcaac aaaaatcaat
     4081 ctggtatcat ttatgcagca acgagaaaag aagtagaccg tctctatcat ttgcttaaaa
     4141 aattcgattt ttccgtgggt agatatcatg gtggtctaaa tgagaatgag cgaacagaaa
     4201 tgcaagaagc ctttttatac gatcgcttgc agctgattgt agcgacaaat gcatttggta
     4261 tgggtataaa taaaagcaat gtccgttttg ttatccatta tcaaattcct ggctctctag
     4321 aagcatacta tcaggaagct ggtcgggcgg gaagagatgg tctttccagt gaagccatcc
     4381 tgcttttctc tcctcaagat atccaagtcc aaaagttttt tatccagcag tcccaaagag
     4441 aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa tacgtgtata
     4501 ttgagtcctg tcttcagcaa tacatcctaa actattttgg cgagacaagc agtccgtgca
     4561 accgttgtgg taactgttta gatgacagag aaatagtaga agtaacgaca gaagcacaga
     4621 tggttctttc ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg ctgatgaaag
     4681 tcttagcagg atcgaaagaa caaaagctgc aagctttagg ttttggacat ttatctacct
     4741 atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa tatttgatat
     4801 cgaatggcta tcttctgacc atcaatggtg agtatcctgt tttgaaagta acagaaagag
     4861 ggatacaagt attgaaagga caagaatctg tttatcgaaa ggaacccaag aaagtccagc
     4921 agctatcgga tgaagaaacc gatacactat ttgaagtgct gcgggaactg cgaacggatc
     4981 tagcatcaga agcaggcgtt cccccgtatg tcgtattttc agattccaca ttgaaagaaa
     5041 tgagccgaat acgtccatca agcagattgg agatgcttca gataaaagga gttggccaaa
     5101 gcaagcttga taaatatggc gaagcctttt tatctcggat aaaaaatgcg gactctaacg
     5161 ttctggataa gtagaaagaa cgaatacgta aaaatgaata aaaacaactg gttaaatctg
     5221 ttttttttgc tccttatgaa aatcaaatat gttataatta aggaattaga atgggaggta
     5281 agcacagatg gattctttgt cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga
     5341 acggatcaaa cgattaatcc gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc
     5401 cttcgaagaa gtggtagata cacagatgta cggcttttcc cggcaagtcg gttttgctgt
     5461 ccgtatagga gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc tagaacgaga
     5521 attgaatcag ttatatagtg aggtttacca agaaatttat gataaaaaag aagttggcaa
     5581 ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc tattagacta tagcatcttg
     5641 attccctatt taattttatg tgtattggga ttgatcatgg tatatagttc tacgtcgtat
     5701 ctattattag aaaacggaca aaatccgtcc gcatcagtca ttaaccagag tattttttgg
     5761 gtgcttagct tgattgctat cgcactactt tataaaatga aaacagatgt tttgaaaaac
     5821 cagcgtttga tcatggcagc catcgcagtt ttgacgattt tgctgctgat tgttgtgttt
     5881 tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa
     5941 cctgccgaat acctaaaaat catttctatc tggtacctgt ctctgacttt atctaaaaga
     6001 caaaacagtg tacaaaaaga ttttcttggg actgtgaaac gtccacttgc aatggttatc
     6061 ggacttactg cattagtcgc tattcttccc gattttggta atgcggcagt tatctttttg
     6121 attattttgg ttctgctttt agccagcgga gtcaattatg tgtatacttt gattgtagga
     6181 gtaggaggct tttgtctaag tacttttacg atctggctga tcaatatcac aaacggaaag
     6241 atcttacccg gacgtttaca gtatatctat aatcgatttg ctatttacca aaatcctttc
     6301 tcagacgaat taaataaagg gcatcaacta gtcaacggct actatgccat gttcaacgga
     6361 ggactatttg gcaggggatt aggtaacagt atccagaaaa aaggattttt acaagaagca
     6421 caaaccgatt ttatttatgc aatcgtagtg gaagaactgg gagtgattat ggggattttg
     6481 atattagcgt tgctattctt tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa
     6541 gatccgttca attcgctttt gtgcattggg atcggcgcaa tgtttttgat ccaagtattt
     6601 gtcaatcttg gaggaattac tggcgttatt cctttgacag ggatcacgtt cccattttta
     6661 agtcaaggag gctccagttt gctgatgctt tcgatttgtg tcggctttgt cctcaacatc
     6721 agtgcggatg aaaaacgcaa gagcttaggt ttataataaa aaaagatgta ggagtggttc
     6781 gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg
     6841 tacagaatta ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat attccgtaca
     6901 ccgtttcaaa gcggatgaag catatttagt aggacaagga aaaaaaccga ttgatgcgta
     6961 tctagatatt gaagggatca tttctatcgc gaaagaatgc ggagctgacg ctattcatcc
     7021 gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat
     7081 catttttgtt ggacccaaac tgcatcattt agatattttt ggagataaaa tcaaagcaaa
     7141 agctgcagct atcgaagcgg ggattgcttc gattccaggc acagatggac caatcgcttc
     7201 tatagacgat gccttggagt ttgcgaaaca atacggttat ccgattatga tcaaggcagc
     7261 tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg caagagaggg
     7321 ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag tgtatgttga
     7381 aaaatacatc gctaatccta agcatatcga agtacagatt ctcggggata cgcatggaaa
     7441 cgtcatccat ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga
     7501 agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt
     7561 ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg
     7621 agatgatttt tactttatcg aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga
     7681 aatgattaca gatattgata tcgtaacaac gcaactgttg attgctcaag gtttggatct
     7741 gcataaagaa atcggattgc ctcagcaaga aggaattaaa ttaaatggtt cagccattca
     7801 atgccgaatc actacagaag acccgttgaa taacttttta ccagacacag ggaaaatcga
     7861 tacgtatcgt tcacctggtg gttttggcgt acgtctggat gtcggaaatg cttacgcggg
     7921 ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc atggtgctac
     7981 ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt
     8041 gaaaacaaac attccattta tgctcaatgt catcacacat cctgaattcc aatcaggaaa
     8101 tgcaaaaaca accttcattg atagtacagc aacattattt gaatttcctc gactacgaga
     8161 ccgcggaaac aaaacgatga aatatatcgg tgaaattact gtgaatggtt ttccaggtat
     8221 cgaaagtggc gaaaaaccat tttatgaaga accacgtatg cctaaagatc taatcacaag
     8281 aactgattac gtaacggcta agaatgtatt agatgcgaag ggtgccgatg ctttggtcga
     8341 atggatcaaa ggacaggaaa atttgttgtt gacggatacg acatttagag acgcccatca
     8401 aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag cagattgcac gtttgactgg
     8461 agaaggtctt cctgaactct tttcaagtga gatgtggggg ggagcaacat ttgatgtggc
     8521 ttaccgcttt ttgaatgaag atccttggca acggttgaga aaaattcgtt ctttaatgcc
     8581 taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc
     8641 tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg
     8701 aatctttgat agtttgaact ggactccaca aatggaaaaa agtatccaag ctgtacggga
     8761 tactggtaaa atcgcagagg cagctatttg ctatactggc gatatcaacg atccaagtcg
     8821 ggcaaaatac aatgttcagt attataaaga catggcaaag gaactagaac aactgggtgc
     8881 gcatatcatt gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag cttatcgttt
     8941 gattagtgaa ttgaaagaaa cgacagatct gccaatccat ctgcatactc atgataccag
     9001 cgggaatgga atcatcactt attctgctgc ttcaaaagca ggagtagaca tcgttgacgt
     9061 ggcaatgagt gcaatgagtg ggaatactag tcagcctagc atgagcagct tgtattatgc
     9121 actggtcaat ggtccacggc tgccagaaat cacgattgaa aatgcacaga aattaaacca
     9181 ttattgggaa gatgtgcgca tgtattacaa accgtttgaa aacggcttga atgctcctga
     9241 aacagaagta tatatgcatg aaatgccagg aggacagtat tcgaatctac agcaacaggc
     9301 aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa caaatgtatc atactgtcaa
     9361 cttgatgttt ggcgatatcg ttaaagtaac tccatcatct aaagtagttg gagatatggc
     9421 actatttatg gtccaaaatg atcttacaga agaagatatt tatgaaaaag gcgaaacact
     9481 aagtttccct gaatctgttg tgactttctt ccaaggagaa ctagggcaac ccgttggcgg
     9541 atttcctaaa aaattgcaaa agattattct taaaggcaga cctgcattga atgaacgtcc
     9601 aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat
     9661 tggctatgaa ccaaaacaag acgaagtatt aagctatttg atgtatccgc aagtcttttt
     9721 ggactatcaa aaagcatata atcaatttgg cgatgttaca ttactggaca caccaacatt
     9781 tttccaaggt atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct
     9841 tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg tactgttctt
     9901 caatttgaat ggacaaagac gtgaaatcac tatcaaagat aattcgatca tcagtacggt
     9961 ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg
    10021 ttctgttttg gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat
    10081 gattactgaa gcgatgaaaa tggaaacggc catcgaagca cgttttgacg gagaagtcgc
    10141 acatgtatat gtgtcttcag gagacactat ttcgtctggt gaccttttga ttgaagtgac
    10201 tgagaaataa taagcgaaag gtggggaaac atgaaaagac tgggtttgtt ttggctgatt
    10261 ctgctattag ttattgtggc attctacatg gaaccagtct tgtttccgcc aaaacttgaa
    10321 gtgaaggaaa actctgatca acaaatatca aaccaaatga caacgtggaa ataccaggag
    10381 cttaaagcaa ctggtttttc tacttatatc ggaaaaccga tcgatcagct ggaaaaggat
    10441 tttggccaag cctatgatcg attaactagc ggatttggtt ttgaaacaag atattatgaa
    10501 aataaagcta gtcatctcac atttgaagcg aacatccaag acagcagagt cacaactgtt
    10561 aaagtactgc aaacagggcc tgctgatatc gcgccgtttt atttagggat gacgatgcaa
    10621 gatcttacga aaatcactac tatctatact aatttcactt ttgagtacca tgatacagaa
    10681 gttggaatcg aactaatgga agaggatatg aattatcgtc cgctaattgc ttttgataac
    10741 gacacttttg ctattttatt ttttgatcaa acaacaaacg gcctattttc agttgtgtat
    10801 ttagataaag atagtctttt gaaactattg ccatatcaag tgtttggtga aggccttcca
    10861 cattatcaag cagataaatc ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa
    10921 agtacgactt tattaaatat gcttagaaga gaagacgaac ttcccatata caatcaaaca
    10981 cttgcctttc aaaaccacag tcagctgcta ctacatgatt ttttgacaag gcctgaggaa
    11041 atattgtcag aggaacgtat ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca
    11101 gtgaaattct cactgacgaa tgatgaattg gagaagttaa tcagtaaaca aaaattagaa
    11161 aatactagtg ggctatttat tcatccggcg attgacgcta ctttttcgtt tttatattgg
    11221 tacagcgatc cttacaacca tgaccgtttc atggacccgg gaacggattc tgtaggcatt
    11281 gctttttcta aagaaaatat gttagtctta ttgcaagaag aaaacggaga aagtagtgat
    11341 agcagtgatc gttaacgaag agctgtttca actggaggat caatgcctcc aagtcgctga
    11401 aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa cataaacagt caatggaaga
    11461 atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct aagaaagaag catttgaaaa
    11521 tgtagcggca tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa
    11581 agcaaaaaga cagcttgata tggatgagca tgttgcagct ttccgatata gtgaaacaca
    11641 gctgcaaagt ctgttggatc aaattgtcca agtacttgcc gaagaagttt ctaatgaaat
    11701 caaagtagat gcaggtagcc cgttttttga aacgaaaaaa cactttggat gcggaggtaa
    11761 ttgtcatgca attagacaat agtgaatttg aaataacaaa acgacgcggg ctgatcgtat
    11821 gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct
    11881 caagaagaat gaaatatgtc gtgctttact taaatgaaga gacattggaa gaaacagaag
    11941 aacgaatccg taaattacat tttgttcgaa acgtagaacg ttcttaccga ccggatatcg
    12001 aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa
    12061 ttgaagagct aaatacagaa atccgattag ctgaacatct ttaaaaatct cttatgagca
    12121 gtttttaaat aaaaaataac ttggataacg atcgattttt gatcattgtc caagtatttt
    12181 tttggtatac tagtaccagt accatttcta aggagtgagt agatgcggat aatatcagga
    12241 aattatggcg ggcgccgttt aaaaagttta gcaggggcga acaccagacc aactacagat
    12301 aaagtaaaag aatccatatt caatatgatt ggaccatatt ttgatggaga gacagtattg
    12361 gatctgtttg caggaagcgg tggtttagct atcgaagcag tatctagagg ctgcagtcat
    12421 gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt
    12481 acgaaagaac cagagaaatt taccacaata aaacttgatg cggacaaagc aatcgaacgg
    12541 ctagcagcgg agaaacaaca attcgactac ttgtttcttg atccgccata tgcaaaacaa
    12601 aaaataatcg atcaaatcca taaaatggaa gaactgggac ttttttctaa aaccgcagtt
    12661 atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa
    12721 ataagaaaac agatttatgg cataacggcc gtaactatct atagaaaaga gggaaatcaa
    12781 tgaaacgtgc attattccca ggaagttttg atccttttac aaaaggtcac ttagatacgg
    12841 tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg tgtttttatc aatacttcaa
    12901 agaaaagttt gtttccccca gaagaaagaa tgacattgat cacaaaagct gtttctcatc
    12961 ttcctaatgt aaaagtcatg catcaagaaa atcaattgac ggtagaaaca gcaaaagaga
    13021 ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa agactttgaa tatgaacgag
    13081 agattgctca gatgaatcat catctatatg atgaattgga aactgtcttc ttattagcaa
    13141 aaccagaata tagtcacgtg agttccagta ttttaaaaga agtactgcac tttggcggag
    13201 atgtgagcag ctacttaccg ccagtgatca acgaagcttt agctggaaag cgtgaatcaa
    13261 atgaaagcta aacaatggtt gaaaacagta ctgattatca tatttggaat gttgatcatt
    13321 ggatttgtag tagttccctt gccgtattat gtagaatcac cgggagccac gatcgatctg
    13381 aaagaactaa ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt ctctcttacc
    13441 tctgttggta tcaggcaagc tacaggtttc ttagctttaa aagctaaaat gacccctttt
    13501 gaagaattga tcagtgaaga agaattgact ggtggggcga caaatgaaga atataatcaa
    13561 atgcaacagt attatatgga ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt
    13621 gcagacaagc cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca
    13681 tcgaatttct ttggcaagct ttcagttgga gatacagtaa caaaagtaga cgggaaaagc
    13741 tttcaaagta cagaagcatt tatggattat gtaaaaagcc aaaaagttgg acaaacaatc
    13801 gaaatcagtt atttgcataa tggtgaagaa aaaacagctt ctggtgatct gatcgagtta
    13861 ccaacagata aaaaggcagg aataggtatc acgcttactg accataccga gatcgaatca
    13921 gatacagatg ttcgtattaa ttctggctct atcggtgggc catcagcggg attgatgttc
    13981 actttagaaa tctatgaaca agtcactggt aaaaacttgc gacatggtaa gcaaattgca
    14041 ggaactggaa cgatcaacag tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa
    14101 gtggcaagtg cggataaagc tggcgtagag attttctttg caccagatga tacgatttca
    14161 gacgatgtta agaaagaata tcctgaaata aaaaccaact acgaagaagc aaaagctgcc
    14221 gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt
    14281 gactatctag agaaaatgaa atgaaactaa ataattaaag aggggttgtg acacgagttc
    14341 tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa
    14401 attgacgtaa ttagatgttg aagggacgat tttgccagag ctcactgttt cttcaatttt
    14461 aaatgaaagg agtaatgaat gatttttact ggtcattctt tgaaaaaaga acaatgatag
    14521 aaaaaatcag caagaaaata ctagcttcac ccaaattgat catttggatg tctatgatag
    14581 ttcttatctt ttgtatcgca agtggttttt tattatcaaa tttgctattt tatagagaaa
    14641 cagaagagac tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga gaagatccat
    14701 cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac accgatatca
    14761 aaggaagtgt gaaggagcct ggtatctatt ccttttcttc agaagaacgg gtctatgatg
    14821 tactaaagcg tgcaggtgga ttattagaag aagcggatag cgatcgaatc aacttttctg
    14881 cgaagataga agatcagcaa gtcctatata tccctgctgt aggtgaagag ctgccagaac
    14941 atcttgatca gttggcatcc tcggaaggaa aacagcctac agcagattcc gagcctccca
    15001 aaataaatat caatacagcc actccatcag aacttcagca gattcctggg atcggatcag
    15061 tcaaagcgca agaaatcatt cgatttagag aagaaaacgg atccttccag aaagtggaag
    15121 acctgcagga gatttctggt atcggtgaaa agacagttga aaaactaaaa aacttcgtta
    15181 caattaaata gaatgattca aaatcgactg gcacgagcaa taaatggacg ctaatggata
    15241 ctaagaataa tccaattcta agagcttggc aggacgagga ggtagtattt ttggatagga
    15301 aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa
    15361 aataaaatat ctgggattca tattatgaat acggctgtca gcggcaataa cagtcaagct
    15421 ctacgagaac gctttgccaa agatgtactt agctatacag cagataaagt atttattctc
    15481 attgggacga atgatctagc agagcataaa caactttcta aagaaacgta ccaaaaaatc
    15541 tgcagtggat gatagttcag ctgaaaaaag actatagttc tgatcatgct ttctttatca
    15601 cgccaccagc agtcgatgag acaaaacaaa aatatcggaa taacaagttg atcaaagaat
    15661 atatctctgt tctcacagaa gtagcaaaac gccatgattg tgaagtatcg atttttatac
    15721 ggaatttctt gaacaaatgg attcgaagaa tttttataat gggctaatgg atgacggact
    15781 tcatttcggc agtgaagggt aggatctttt gtcagacagg atgattcgtt atatttaaag
    15841 aatattttgt ttaagcaatg agtacttttt aatcaacttg aaaaaacgga ggaatataaa
    15901 acatgttaga aaaatcttca aaagcatgcg atacgaataa agaaaaagtt actgataagt
    15961 cacataatga acgcattcct tgggaccagt actttatggc tcaggcagta ctgttatctt
    16021 tacgtagtac gtgtacacgt ttagaagtag gagcgacact cgttcgagaa aaaaggatca
    16081 ttgctggcgg ctataatgga gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt
    16141 acgtcgtgga tggacactgc ttgcgaacga tccatgcaga gatgaatgca ctcctacagt
    16201 gtgcaaaact tggtatcccg acggaaggtt cagaaatcta tgtcacacat tttccatgtt
    16261 tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat ctccatgact
    16321 atcgaaatga tccttatgcc caagcgttga tcgaacaatt aggtgtatct gtacaccaag
    16381 ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga attaacagga aaacatgtct
    16441 agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt
    16501 atctgtttgt tgtattacac tgacaattgg ggttatcgtt tgcttcttgc cggaattttt
    16561 ttatggtttt cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt gtgtagcata
    16621 acggtatcca tttcttatag gctacaagag tattcatttt tagaggaaaa aacaacatct
    16681 gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg gtaatttttt gacaatggaa
    16741 ggaaggttat cagagaataa acaaaagctc cagctttctt atcaagtaaa cacagaagca
    16801 gaattgaaaa catggcttaa cttccgagga aagggaatag aattgactgc tgaaggcgag
    16861 ttcatctctc cagcaaaaaa gcggaatctt tataattttg accaagaagc ttacttcagg
    16921 aaccatagaa tttctggacg gttccaaatt caaaagattg aacagatcac catgcaaaat
    16981 cactggcgaa actggcttca gagaaaaaga ggatctttta tctcctttgt ccaatctgaa
    17041 tttccgcaaa aaacaagtgt atatattaat tctttattat ggggctataa agatgcagga
    17101 ttcagagaac cagaagaaat ttttagggag agtggtttgc tgcatctgtt cagtctatcg
    17161 ggtctacaca tccaatttta tttagggtgg ctgtattatt tatttcgacg tatcggatgg
    17221 ccactacacg tcagtattat tccgttgagt ctactaacta tctgttatgt ttctattgca
    17281 ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa
    17341 ttgctgaata agcattattc caccatggac ttatttgcag ttgtcttttg gcttctttta
    17401 ctatttgatc ctcgatttct ttttcatgcg ggtggacagc ttagtttttt catgagtttt
    17461 ttgtttatcc tgatttcttt tgatggagat tggttgaaaa aaacggcctc tcacgtcctg
    17521 tttactatga tcacgatgcc gttgattgta tggcattttt atgagtggcc tattttaggt
    17581 agtcttttta cattactgat tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta
    17641 tttctcacac tcttcaatcg ctggctgccg gaaactttac ttttgctgat agaagaggga
    17701 ttaatcttat ttgagcgaat aatcgatttt tcttcagggt cgtctctcat tataggacgt
    17761 ttgccccttg tgctatctgg gatgtatatt ttcggattac tgatttgtat ggataggttg
    17821 aaagaacaac ctgtgaaaca cttgttttat gttggtcttt tttcttttat ttttttgact
    17881 ttgccatttt tacgcttttc ttcaacgatc gcttttatcg atgtcggtca aggagacagt
    17941 atctttttgc aaagtccttt tcgaaaagaa acgattttga tcgatactgg aggtcagtta
    18001 cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc
    18061 gttccatatc taaaaggtca ggggataagt gtaatcgata aactcatctt gacacacgac
    18121 gatacggatc atgtgggaga attacccacg ttatctcagc attttactat caaaaaaatt
    18181 tatatcggtt ggggcgctgg acgaaatgag ccattacaaa ggcatttatc tgaagctcaa
    18241 aaaaatggta ctgagatcat tgaaatcaaa catggggaca ggatcagcgg ttattatgat
    18301 ctttatgtat tgactccttt tgaaaaggga gagggaagaa atgaagattc gattggtcta
    18361 tggatggaat ataacaatag gcgcttttta tttttaggag atttgaatca agcaatggaa
    18421 aaacaattgc ttttgattta tcctaatctg aaagccgatg tagtcaaatt aggtcatcac
    18481 ggtagccgta cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa acatgggatt
    18541 atatcctgcg gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt ggagacacta
    18601 gaagaaaatc agataaaaac atggcgaacg gatcaacaag gaatgatcac ctatcgctgg
    18661 catccagttt ttcatcctca tgggctagtg gaaacaatga tagactagta attctcatct
    18721 gctcatggta ggataatttt tgaaagtggt gagtaatttg agtatacaag cagagttaca
    18781 aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag aaaagtttct
    18841 ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa ttagagggcg aagatgactt
    18901 aaatttttta agttttgaca tggaaaatgc aagtttagat gaggtagtag ctgaagcaga
    18961 gacgttgccg tttttcggag atcaacggtt agtattcgta gagaatcctt attttttaac
    19021 aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat ttattgacgg cttatttgaa
    19081 agaaccatta gaatcgactg tattagtatt ctttgcgcca tatgaaaagt tggatgaacg
    19141 gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa
    19201 tgaaaaagaa gtacgtcaat atctgatgaa taccttagag aattcctcaa tcaaaatgga
    19261 acgaagtgca attgatttat tcttgaggtt gaccgactta gatttatcca aaatgatgag
    19321 agagttacag aaattggtcc tttatggaca aaatcaacag caaatcacgg taagagaagt
    19381 agaagaatta gttcctaaaa cgttagaaca taatattttt gacatgaccc aatatatttt
    19441 aaaagggaag acggaacaag ctttgcgttt atacgaagat ttagttatgc aaggggaaga
    19501 aacaatcaaa attaatgcga ttttgctttc tcaattacgt ttatttttac agacaaaatt
    19561 tcttgtaaag attggttatc agcaggcaaa tattgctgaa acattgaaaa tccatccata
    19621 tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat gaacatttgc tggtacagtt
    19681 gtttgatcaa ttagttgaga tggattatca aatcaaaacg ggacaaatcg aaaaagaatt
    19741 aagttttcaa ctattcgttt tgcgcacagg gcagttgtta agaaatcgat aagaagaaag
    19801 acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa
    19861 atcggccgaa gccgattctt ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg
    19921 ttagctttgt ttttgtggat tagacctttt gattcagcca tatcaatagc tttaacagct
    19981 tctttatata acgcatctac gttgtcagca cctgaagcta cagcatcttc aaattttttg
    20041 attgcagtac gcatagcgct tgtttgagat gaatttttca cattagcgtt ttcgcttgta
    20101 cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt tcacctccga taacttaagt
    20161 tcttacgcgt gttaccgcat aattcaacat tcattattat acctaaaggc agcgtgcttt
    20221 gcaatatgaa catgacaagt ttttttcctt aacagtcttt tattaggatt ttttttcagt
    20281 aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg
    20341 tcaaaccaaa ctagattttt atttatctat ttaaacaaag tttgaatggg aatgtttggt
    20401 actaagttat tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa agggatacga
    20461 ttccctgttg ttctttgttt ttctattaag aaactgcagt tattttataa ccagtatcct
    20521 ttgatcagta aaggctattc tggtgtaccg attattttaa tgtattcatc gatatttttt
    20581 atcaacattt tttttatgac caactggttc cgacggttcc atttgccact tacttccagc
    20641 acatatttat ttaggggcaa acgtaagata tattttgcca aatagcgatc tttaagcagg
    20701 ccattgatca cacggtcctc aagctgcaag ttgatcccag tcagcatcat tggctgttct
    20761 tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt ccacaagcgt cttcctttct
    20821 tttcaaagta acctgaaaac aggcactaat tcttccattt tgatattcgt tgaagtggat
    20881 gcgatcattc tttcttttgt ccactgtaat ctagtataac aaaaaagagc agaagagcca
    20941 aaaagaaaaa agagcatcga attctatagg aagtctcatt ggcacggcct aaattattac
    21001 aaaacattga ttaaaaaaag cccctgcatc gcaggagcat cccctatatc gttttaatag
    21061 gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat caggaaataa tagataagct
    21121 aagcccagaa tcaaaagtac cgctagaaga aaccagccga ttgttcctat gaaatacttt
    21181 aatttttcga acattgtatc acctcgttgt cagtatatca cggatcagta aattgggggt
    21241 gttaagaatc tcctaaaata gacaaaattt gttctatttt cctaaagcaa atacttgtaa
    21301 gatacagtaa gtgataccag aagcaatgac cggtccggcg gcgattcctc ttaagaagac
    21361 aacacctaaa atcgtgccga aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa
    21421 tcctacaccc ttggaagata agacagccac tagcccaccg caaaaaacag ctacccagcc
    21481 aataggggat ttaaaggctt ctaatagatc acgtagaccg atttctcctg ttgcgatagg
    21541 gattagaata gccgctgtga tgatcgtcac cccaatattg attcctttgc cttgaagcca
    21601 agggaaaaag gaacgggagg ctggtatgag cttcataatc aaaacaaaag ctgccgctat
    21661 catcagactt tgatttttag ctaaaattgc tacaagtaaa ataattccta aaaaaagcca
    21721 actttccatg aaatatattc ctcactttct tcgaaacaat cataacatgt ttttagaaag
    21781 ttacaaaagt aggaattcat ccttcaagac ctattcataa gctgtttttt tcataaaata
    21841 aacaaaatca taaaaaaaca ctttgcttat ttgacagaag acagccgcgc tttgtatagt
    21901 ggacaagact tacatttttt tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc
    21961 aagccattca gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga
    22021 tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa taacaagaat cgaaaaaata
    22081 atattttgtt tgatgatccg ccgcattttt aaagatagcc gatgactgta gacaaattta
    22141 tttaagtcgc ttttcattaa gacgatgtct gcgatatcca tagcggcatc tgtcccttgc
    22201 cccatagcga cacctaaaga agcggctgct aaagcaggcg catcattgac tccatcacca
    22261 accatcgcat ttacttggta aagttcattc tcttttttga ctagttgtgt tttttcttcc
    22321 ggagtacagc ttgcatagta atgttcaatc cctacttgtt cagcaatggc ctttgctgtg
    22381 ccagaatggt cgccggttat catacttgta tggatgtttt gggatttaaa gtaggaaatt
    22441 acttgttggg tatttgcttt tggaatatct agcagaccta aaacagcaat taactgctga
    22501 tctttagaaa gataaatcac ggttttgcct tgattttcca aacgttcgat catttccgca
    22561 atctctttgc tgattatcat cgtctcagga tcatatgcgt gcttgccaac tttccattgc
    22621 gcacctaaat aggtagtttg taatccaaaa ccagtaattt cttctattgg taagtattgg
    22681 atttcttcag gtattgtgca atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc
    22741 gttttttgtt ccatagcaac aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag
    22801 ctgtcagtta caactggttt tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct
    22861 tttaattcag aaagcttttc taaatggata ccgcctttga ttaaaatccc attttttgca
    22921 gaatttgaaa gagcagctag agaagctggt gtagcagatg cgacaagcgc acaaggagaa
    22981 gccacaacta ataataccat tcctcgataa aagctttcat cccaactcca acctaggaaa
    23041 aagtggggga gaaggatcat tagaggaaca gcaagcaaaa tcaattttac ataattattt
    23101 tcgattcgtt cgataaatga agctgtttga gttggcgtat tctgcgcttc ttctactaat
    23161 tggataatct tagcaaagac agtttcggaa ctcgtttttg ttactcgaat cgtgatggga
    23221 tttccttgat tcagcgttcc tgcatacatc tcatcgccta aatatttttc tacaggcaca
    23281 gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa aaagccatct
    23341 atcggaacag ctgctccttt agcgacaaat accaagttat ccacctttag ttgagaaaca
    23401 tctacttctt gcatttgtcc attttctttt aataattggg ctttttgtgg ttgaagattc
    23461 atcaaagcag taatttcttt ttgactttta ttagttgtgt attcctctaa tgcaccgctt
    23521 aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa acaggcacca
    23581 atagcagcta aagccatcaa taaatccaca ttcagtgtcc gatcattcca caattctttc
    23641 aagccttccg atgtttgttt atatccaccg atgataatgg cactgatata aaaaagtggg
    23701 gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga tcataaataa aaggcagccg
    23761 attgttgctg caaaagcttt tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac
    23821 aaggatagca taataaaaat gtaattgata ataataacag ctaaatgaga acgataatgg
    23881 ttatcatttg gtgattaagc ttgatgttgt ttttattatt tataatgatt attattaaag
    23941 aaaataaaaa accagaacat ttc
//
LOCUS       DACDKL010000023        22784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDKL010000023 DACDKL010000000
VERSION     DACDKL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.023"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            474..725
                     /locus_tag="IV329_000733"
     CDS             474..725
                     /locus_tag="IV329_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939937.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IV329_000734"
     CDS             759..1037
                     /locus_tag="IV329_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939938.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IV329_000735"
     CDS             1634..1891
                     /locus_tag="IV329_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939939.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IV329_000736"
     CDS             2004..2615
                     /locus_tag="IV329_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939940.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IV329_000737"
     CDS             2794..3351
                     /locus_tag="IV329_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939941.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IV329_000738"
     CDS             complement(4050..4571)
                     /locus_tag="IV329_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8939942.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IV329_000739"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IV329_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8939943.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="IV329_000740"
     CDS             8651..9538
                     /locus_tag="IV329_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8939944.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="IV329_000741"
     CDS             9535..10704
                     /locus_tag="IV329_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939945.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="IV329_000742"
     CDS             10728..12083
                     /locus_tag="IV329_000742"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8939946.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IV329_000743"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IV329_000743"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8939947.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="IV329_000744"
     CDS             14369..14659
                     /locus_tag="IV329_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939948.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="IV329_000745"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="IV329_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8939949.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="IV329_000746"
     CDS             15700..16359
                     /locus_tag="IV329_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8939950.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="IV329_000747"
     CDS             16516..17448
                     /locus_tag="IV329_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939951.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="IV329_000748"
     CDS             17602..18891
                     /locus_tag="IV329_000748"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8939952.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="IV329_000749"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="IV329_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8939953.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="IV329_000750"
     CDS             20471..21058
                     /locus_tag="IV329_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8939954.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            21423..22751
                     /locus_tag="IV329_000751"
     CDS             21423..22751
                     /locus_tag="IV329_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939955.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         7581 a         3882 c         4877 g         6444 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttctagat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgagta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa atgctttgtc aaatgctctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aacatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcctg ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacgtg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagcgtg
    19801 ccacctcagg gaggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagtcttttt
    21121 ttgagcttaa cttacttttg tttcttgtct tttttctttt cattttgtgg ctgtggtttt
    21181 agaaatcgat cgatctcttc ttttggtgca actgtttttt ccggatcgac agcaaatgat
    21241 tcgaaaagtt tatctaaatt atctgtacgt tcaaatttca accccattgt aaacacctct
    21301 ttgtttcttt ttacttagtc tatcatgtaa tcgtccaaat tggtttctaa gacataaagt
    21361 tttagttttt ctttatcgtt ggttataatt aaattataaa aagattttaa agaggtgaga
    21421 aaatgattgg cgaagataaa ttattgatga ctatccagct cacagactta aatgaagaag
    21481 aaaaaaaaca ggaaataagc gattgggcta ttttgacacg tctgttgatc cagccaacat
    21541 ttatccgctc tttcacacaa caagcggatc cgtatgatct tcgaaagctt caagaaaaaa
    21601 taatgctagc atcggtaaga gacgaaagtt ggttagttgt aggaaatgac gagaacgaat
    21661 gtagttttcg cttggaagat aatcaattat tgataaaaaa tgtattgtct atctctgttt
    21721 tcaaagaaaa gcaatttttg attcgagact tcattcagaa aaaaatgatt gctcatgggg
    21781 tgttcgccta tatgcgagct tatagcgagt ttatctatca caatacaaaa cagatctctc
    21841 aacggctatt gtttgagaaa aaggatgaaa tcgaacattt acctaaaatg aaacaacaaa
    21901 acggggaagt agtagtcgat tgcaaccaat ttccaggata cgatcttttt taccaaggtt
    21961 tatgtttcac ttcgtgttgg gaaatgtatt atagtcgcta ttatcatcaa atcatcccta
    22021 aaccgatttt tctcgacatc caacaagtag aaaaagtgaa ggaattagaa aacgaagtca
    22081 tatgtatcca gctttatcga gaccccttta actggcaaaa gccaaacaat caaatgtttc
    22141 aatcctattt tagggaccag cttgggtttg atcatcttgc ttgggacaat ggtgtcggtt
    22201 tattgaaacc gccattcgtt gaatacattt acactgacca tacgatccaa tctgtccagt
    22261 atcagaatgc acaaatgcaa cctgttccta aaaagaatgc ttcatttttt gtaacaaaaa
    22321 gttatgatat tcaacaagga gattataaag aaaggcgtgt tcgcggaaca ctaaatgctc
    22381 aggcgtattt tccttgggtt gacgagaatc gctcgcgcat gatgtgctat aaagttatcg
    22441 atccgactgt tgctttggat aatggaatcg aagcttattg ttattatatt agggaatatc
    22501 tagaagtaga ggtaacagat gaaaaatatc aagagtatct tttatcctta agaatctatg
    22561 ttccttcaga atccctgtca gaattaccgt taaaggaaat caaacataga ctctctgatg
    22621 ttaccttcaa acgaataaag aaaaagcggc gaagtatcca attcgatgtc aagaaaggtg
    22681 agcagcatct gcgtgtgcaa tttttagatt accgagaatt agaacaattg attactttac
    22741 aaaaaatatg aggtgaagaa aatggataaa caactctatt atcg
//
LOCUS       DACDKL010000024        22595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDKL010000024 DACDKL010000000
VERSION     DACDKL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.024"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IV329_000752"
     CDS             133..3660
                     /locus_tag="IV329_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8939956.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IV329_000753"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IV329_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8939957.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IV329_000754"
     CDS             7448..7789
                     /locus_tag="IV329_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8939958.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IV329_000755"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IV329_000755"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8939959.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IV329_000756"
     CDS             9146..11566
                     /locus_tag="IV329_000756"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8939960.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV329_000757"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV329_000757"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8939961.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IV329_000758"
     CDS             13000..13737
                     /locus_tag="IV329_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8939962.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IV329_000759"
     CDS             13751..14566
                     /locus_tag="IV329_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8939963.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IV329_000760"
     CDS             14579..15226
                     /locus_tag="IV329_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8939964.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IV329_000761"
     CDS             15242..15898
                     /locus_tag="IV329_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8939965.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IV329_000762"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IV329_000762"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8939966.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IV329_000763"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IV329_000763"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8939967.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IV329_000764"
     CDS             18240..18758
                     /locus_tag="IV329_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8939968.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IV329_000765"
     CDS             18865..19365
                     /locus_tag="IV329_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8939969.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IV329_000766"
     CDS             complement(19645..20565)
                     /locus_tag="IV329_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8939970.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IV329_000767"
     CDS             20730..21743
                     /locus_tag="IV329_000767"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8939971.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..>22595)
                     /gene="fabI"
                     /locus_tag="IV329_000768"
     CDS             complement(21853..>22595)
                     /gene="fabI"
                     /locus_tag="IV329_000768"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8939972.1"
                     /translation="GKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMKKSLV
                     KLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNVSDISR
                     DGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKASLEA
                     AIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTIEEVG
                     NACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7522 a         3942 c         4816 g         6315 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tcctt
//
LOCUS       DACDKL010000025        22430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDKL010000025 DACDKL010000000
VERSION     DACDKL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.025"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..308
                     /locus_tag="IV329_000769"
     CDS             <1..308
                     /locus_tag="IV329_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8939973.1"
                     /translation="LFLFLAFEMHLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFD
                     DIASIWPVLLGAVVVCSIVTMVAAGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            301..1002
                     /locus_tag="IV329_000770"
     CDS             301..1002
                     /locus_tag="IV329_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8939974.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1002..2588
                     /locus_tag="IV329_000771"
     CDS             1002..2588
                     /locus_tag="IV329_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8939975.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2607..2828
                     /locus_tag="IV329_000772"
     CDS             2607..2828
                     /locus_tag="IV329_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8939976.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2825..3586
                     /locus_tag="IV329_000773"
     CDS             2825..3586
                     /locus_tag="IV329_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8939977.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3966..4475
                     /locus_tag="IV329_000774"
     CDS             3966..4475
                     /locus_tag="IV329_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8939978.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4550..5089)
                     /locus_tag="IV329_000775"
     CDS             complement(4550..5089)
                     /locus_tag="IV329_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8939979.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5229..5840)
                     /gene="rpsD"
                     /locus_tag="IV329_000776"
     CDS             complement(5229..5840)
                     /gene="rpsD"
                     /locus_tag="IV329_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8939980.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6156..6953
                     /locus_tag="IV329_000777"
     CDS             6156..6953
                     /locus_tag="IV329_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8939981.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            6981..7616
                     /locus_tag="IV329_000778"
     CDS             6981..7616
                     /locus_tag="IV329_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8939982.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7636..8409
                     /locus_tag="IV329_000779"
     CDS             7636..8409
                     /locus_tag="IV329_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8939983.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8817..9614
                     /locus_tag="IV329_000780"
     CDS             8817..9614
                     /locus_tag="IV329_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8939984.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9592..10005
                     /locus_tag="IV329_000781"
     CDS             9592..10005
                     /locus_tag="IV329_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939985.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9989..12634
                     /gene="mutS"
                     /locus_tag="IV329_000782"
     CDS             9989..12634
                     /gene="mutS"
                     /locus_tag="IV329_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8939986.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12652..14760
                     /gene="mutL"
                     /locus_tag="IV329_000783"
     CDS             12652..14760
                     /gene="mutL"
                     /locus_tag="IV329_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8939987.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14782..15342
                     /locus_tag="IV329_000784"
     CDS             14782..15342
                     /locus_tag="IV329_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8939988.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15427..17415)
                     /gene="treP"
                     /locus_tag="IV329_000785"
     CDS             complement(15427..17415)
                     /gene="treP"
                     /locus_tag="IV329_000785"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8939989.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17638..18351
                     /gene="treR"
                     /locus_tag="IV329_000786"
     CDS             17638..18351
                     /gene="treR"
                     /locus_tag="IV329_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8939990.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18428..18874
                     /gene="msrB"
                     /locus_tag="IV329_000787"
     CDS             18428..18874
                     /gene="msrB"
                     /locus_tag="IV329_000787"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8939991.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19044..19646
                     /gene="ruvA"
                     /locus_tag="IV329_000788"
     CDS             19044..19646
                     /gene="ruvA"
                     /locus_tag="IV329_000788"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8939992.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19659..20660
                     /gene="ruvB"
                     /locus_tag="IV329_000789"
     CDS             19659..20660
                     /gene="ruvB"
                     /locus_tag="IV329_000789"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8939993.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20689..21267
                     /locus_tag="IV329_000790"
     CDS             20689..21267
                     /locus_tag="IV329_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8939994.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21319..21801
                     /locus_tag="IV329_000791"
     CDS             21319..21801
                     /locus_tag="IV329_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8939995.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21918..22292
                     /locus_tag="IV329_000792"
     CDS             21918..22292
                     /locus_tag="IV329_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8939996.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7309 a         4041 c         4847 g         6233 t
ORIGIN      
        1 ttcttttttt atttttagca tttgaaatgc atcttgttga tcctgaaaaa atcggagcaa
       61 ctggtgattt tttattgaat aatctagcca ttttatttgt tcctgctggt gtaggtttat
      121 tggaatattt tgatgatatt gcttcgatat ggcctgtttt actgggagca gtcgtcgtgt
      181 gttccatcgt cacgatggtt gcagcgggaa aaacggcaga aggtgtagaa gctttgctgg
      241 gctatgtccg taagaaaaaa gcagaaaggg tatctgtaaa cgaaatggag gagaatcaaa
      301 ttgactgagt tttttgtgaa tccattattt ggcttaagtt tgactttagg agtgtacgta
      361 ttatctgaca aatttttgcg gaaatataaa ctgcctgttt tgaatcctct cgtattatcg
      421 attgttgtta ttatcttatt tttgcatttt tctgggatca gctataaaga ctataatatc
      481 gggggcaatt ttttgactat gctgattact cctgcgacag tggcacttgc catccctctt
      541 tataaaaact tccgtttact gaaagagaat ttttatcctg taatggctgc gattttagca
      601 ggaatagtcg caaattgctt ggttagtgta gggttcggtt acttcttctc tcttcataaa
      661 gagatggtga tctcattact acctaaatct gtaacgacgg ctatttctgt cgatttatct
      721 catacgatgg gcggggtgaa tgccgtgaca cttgctattg ttgtctctac aggtattttt
      781 ggttcgctga tcgccactca tattttccgc ctattcaaga tcgaaagtcc agttgctcgc
      841 ggtgtggcgt taggaagtac ttctcatgct attggtacag caaaagcaat cgagattggt
      901 gaaatagaag gaatcatttc cggacttgcg atatgcgtca acggaatatt gaccgtttta
      961 ttgctaccgt tattctttca gccttttgct ggcttgtttt gatgcgaaaa ccactatttt
     1021 atgctacaat aaccaaaacg ttcgagcata aaaaaggagg gaaagacgtg tatttgtctt
     1081 ccgagtatct taaacaattt gataaagatc tatattataa gattctcctc ctagaaagtt
     1141 ttgaggatca agcttggcat accgctgcac aattagcgca agttgttcag ctggatgccc
     1201 gcagcgtgtc gaaatacctg aacgagctgt ctaaaaacta tcagcagttt agcggtaaga
     1261 cacacccttt gtttacaaaa aatcaccgtt ctggttataa cttttatgat acgctcgatt
     1321 caattgaaca tgagcgtttt ttgatctatc tcgtgcaaag tacgctgaaa ttccagttgc
     1381 tgcacgacat tttttttgaa gaattccata caatgtacca atttgcgcaa aagcattaca
     1441 ttagtgaatc tactgctcat cgaaaaatca atgaatggaa acagcaactg caaacttacg
     1501 gaattcggct gcaacgaggt acatatatcg ctcaaggcga agaagaaatc attcgacttt
     1561 acttgcatat gacattctgg cagttgtttc gagggaaaat ctggccattt gaaacgattt
     1621 ctcaaatgga cgtaaagaat atggcagaac atatcatggc tttttttaat gttcgtctga
     1681 acgagatcaa aaaaagacga ttggaatata tgctaggtgc ctttttttta agaaaatcac
     1741 agaagcatta tgttgtattg aatgagaaaa aaagacggct gatttctgac aatctgttgt
     1801 tccagcgatt ttgccaggtc atggagccgg tatttcctaa ttattttcaa gtagaagacg
     1861 agttaggcgc attatttctt gtactgatga ctcgtgaaga atattatagt gatcctaaga
     1921 tacggaagaa aatttttgat tttcatcaac aagcaaaaac accgcccttt acagcccttt
     1981 cagaagcaaa agccgctctt tctctttatc aggaagagca ggggctgcca gcagaaaatc
     2041 taaccttcga ggcagaaaac tatttattca gcagtcattt tttcgcctat ctttttccta
     2101 atgcgaaaga aacgattgat ggcaatagta gtgattttat taatcattta gttattgaaa
     2161 acaaggaatt gaaacaatgg ctcgtacatt tttttgaaag tagacacaaa catcccaatc
     2221 atctggcatt taaaaatcat gcgtttttga tggggcggta tctgactgtg tttaaaacat
     2281 taggagcctt tactccacag cttccaaaga tcacgatcct tttaatgaca gatttcccat
     2341 tatttgaaga gcaactgtta gaggagggat tacggacatt tttccgtaat gagtatcagc
     2401 tgatttttct gccaacagat tatcggggga gagaagttga tcttctgatt tcgacaagta
     2461 aagtccaccg caaaccatgg gcggatcttg actattttat tgtgactgaa gaactaaaat
     2521 taattgatta catccaacta tctcaaaaat ttgaaatgat tcaaaaacaa aaactattaa
     2581 aacaataaca aagaaggaga aaaaagatga catttgaaga aattttacca gaattgaaaa
     2641 aaggtgcaaa gattattcgt aaaggatgga gtggatttga actttatgtt gttctcgtat
     2701 ccgatgatga atttgatggc tgtccggtga cgccgtactt attgatcaaa acaagtgacg
     2761 aaggtttttc cagcttcgct cctacagttt gtgatatttt agcagatgat tgggcgatcg
     2821 tagaatgaaa aaccagtatc cagatctagc taaacaggtt gtatttatca caggagctgc
     2881 ttctggaatt ggtgctgcgc aagcccgagc gtttcttgaa caagatgctt ttgtatttgg
     2941 attagatcaa aagttcggta aaatggaaga aatttctttg cgatttcctg aaacttttgc
     3001 ctacgcacta ggtgatgttc gacaaatgaa agaactccaa caagcggtat cgaaatgcca
     3061 aaatcatttt ggtgaagtta cgattttact gaatacagcg gggatattgg atgcctacaa
     3121 acccttgatg gaaactgacg aatcgctgtg ggatctgatt tatgagacga atgtgaaaag
     3181 catgtatcag ctgacaaaaa tcatccttcc tgatatgatc cgccaaaaaa gcggaacgat
     3241 catcaatatg gcttcgattg ctggcttgat tgctggtgga ggtgggatcg cttatacaag
     3301 cgcgaaacat gcgattgtcg gatttaccaa acaactcgct ctggattacg caggacaggg
     3361 tatccgagta aaaggaattg ctccaggagc catccaaaca ccaatgaatg cggctgattt
     3421 tgaagggaaa ggtgagatgg cagagtgggt ggcaaatgaa acaccagtca agcgatgggc
     3481 acagccggaa gaagtagctg agctgacgtt atttcttgcc tctcctcaag caagctacat
     3541 acaaggaacg atcatcccaa tcgacggcgg atggttgtta aaataacctt gtgttttttt
     3601 caagcttctg tatgataaaa ctaaagagag aaacaacggt tgatgataaa aaaagtcgat
     3661 gactgttccc gacattcgat gccgaacagc cagttcaaaa aattcttttt aataatcgtt
     3721 aatattcgaa actaagcggc ttttcacgac ctcttgatat aaacgatatt caaaagcttg
     3781 caactgcggt aacaagtcca gccaaatgaa aaatataagg agctattttt atttgaccat
     3841 tcttattact tgttgcaggg cagctttttc actaccttct gcgacacttc agttggaatt
     3901 ttcttcccat cctttggtcg cggtcattac caagataaaa ttatcttgca aaggagaaat
     3961 agaacatgga aaaaaataat acccgttcag ccaaacgctg gacaacgaca gatgtgactc
     4021 ggatggcgct agtcactggt ttatatgtgg cagtgacgct ggttttgtct gtctttagct
     4081 ttggagtgat tcagattcgt ctctcggaga tgttcaacta tctgtccttg tataacaaaa
     4141 gatacatcgt cgcggtaaca gcgggagtag ctttggctaa tattgcttct cctcttggac
     4201 tgatagatgt tgcagtagga agcgtcagca cgctgcttgt attgcttatc aactataaaa
     4261 tcactagtcg catcaaaaac atgaaaatca aaatggccgt gacagcatgc gtatttgctt
     4321 tttccatgtt tacagtagct ggtcagctaa cgattttata tcaactacct tttttcttga
     4381 attggtgggt gattgcttta ggagaattgg catcaatgat tgtcggtggg ataattattt
     4441 attggatcgg taaaaaagtg gatttgtcaa aataaagatc tgtttaattt aaaatagggc
     4501 agcatggaag agactgaatg ttgagtcttc ttccatgctg cttttttgat taatttgtat
     4561 gaagaaatcg atttagccgt tttttcatca agataccgat ataagctgct ccagttgcct
     4621 tgattgcttc cggcagtaaa aacggcagta gtccactggc aaatgcagtt gtaaaagtaa
     4681 gatcaccact tattttcagc cagatcgttc cgcagatcaa tgcagcaaga gagcccatta
     4741 gattggcaaa gattgcatat aagtaatgag catccgttct ttcgatcatc caaccagtta
     4801 tccacgtttg taagagaaat ccaatcaaat atccgccagt tggcccaaat aaaacagcta
     4861 tacctccagt catccctgaa aatacaggaa gaccaatcaa gccaagtaaa agataaactc
     4921 caacagatat caatcctagt tttcttccga gaaaagttgc tgttaatcca atagcaaatg
     4981 tttgccctgt caaaggaacc aaaccaaaag gaatggtcat ctgagaaagg acagaaataa
     5041 tcgcggcaaa ttccgcagca agtatcaatt ctttcaatgt tcggttcata acgccctctt
     5101 ttcgttaact taatattttt atttggttaa cgaaattgta aaacaaagtg attactttgt
     5161 aaatagaaaa tataaaaaaa gaccagctat ccaatagata gcggtctttt tcaagaaagc
     5221 ttttgaaatt ataagctttg gttgtagtat tcaacgacaa gagcttcgtc gatttctgga
     5281 tatagttcgt cacgttctgg taaacgagtc aatgaacctt ccaatttttc agcgtcaaag
     5341 cttacgaatg ctgggcgtcc aacagttgct tctacagctt cttttactgt tacgatgttt
     5401 tgtgattttt cacgaactga gatcacttga cccacttcta cgtggtatga agggatatca
     5461 acgcgtttgc catcaactaa aatgtgacca tggttaacta attgacgtgc ttgacgacga
     5521 gtcgttgcaa gtcctaaacg gtaaacaaca ttgtctaaac gttgttctag taagatcatg
     5581 aagttaacac catgtttacc ttctttgatt ttgctagctt ttacgaacaa gttacggaat
     5641 tgacgttcgt tcattccgta catgtgacga agtttttgtt tttcagttaa ttgcatgccg
     5701 tattctgatt gtttgccacg gctattaggt ccatgttgac ctggtgcgta agggcgacgt
     5761 gctaattctt ttcctgtgcc tgacaatgag atgcctaagc gacgagaaat tttccatgat
     5821 ggtcctgtat aacgagacat taaaatttcc tccaatgaat ataagttttt ggagtaaaat
     5881 aatccgttga aaaattcata atcatatagt ttgttcttca atcttcacct ttgcagccgc
     5941 ggttacacaa tcgaacccga gacggggcaa caaactaggg atgagttatt atttttctgc
     6001 tgcattattt tacacaaatt acagtatatg atagatcatc cacgaatgtc aagataatta
     6061 gtacataaaa acgctttaca aacctaggct gtctttataa aatgagaggg tatgtgcaaa
     6121 tggaaaaaag cgaaggaagt ttaaggagaa aatcaatgaa tccggtatca ccattatttg
     6181 agaaaatcga tgcttcaatt gaaaagaagg cgaatctttt tgaaaacagc ttttcacgtt
     6241 acgctgttcg agcggttctc gcctgtttgt ttttgacttt aggaacggct gtggcatttg
     6301 cgatcgcgat gaagggggaa gatatcgcgc atggtctagg aaagatttta tacgcattta
     6361 tgttcagctg gggtctggtc atgattctat acatgaacgc cgaattagga acatcgaata
     6421 tgctttatat gaccgtgggt gtttttcggc agagacttgc tttaagtaag gcagccaaga
     6481 ttctatttgc ctgtattttt ttcaatatga ttggcggtat attatgtgga tttctaattt
     6541 ctatgactac gccatttcaa gatttaacgt tggacaactt tatgttagaa tcaatagcag
     6601 taaaactagc aaaaccttca gcaacgattt tggtggaagg gatgtttgcg aatatcgttg
     6661 tcaataccgc tgtactgatc agcatgcgga tgaaagagga tgtcggaaaa gtgatcacag
     6721 tcgtattcat cattttcatt tttgcttttt taggatatga acacgtgata gcgaattttc
     6781 ctgcctttac gcttacttac tttgcttctc acggacagat ggatggaatg acagtcagca
     6841 atgtgttaca taatttgttt tttgctttgg caggaaatta tattggtggc ggtttagtaa
     6901 tgggactggg atatgcttgg ctgaatcagt caaaatcatc ctatgtggac taaaaagtcg
     6961 ttcattaaag gggaagtgcc atggaagttt tagtttttcc aaaaaatgag gaaaaagcgt
     7021 tacaaatgga aaagtacatg aaaaatcttt tttcctttgc tggagtacct aagccggaac
     7081 gcgctctttt acaacaaccg ttactggatg agagcaaaaa tttatctatt tctgaagttt
     7141 tagcttttgt tcattattat tatgaacaaa tcgaaagaga gtatcagtat gtagcgatcg
     7201 atttagtcca gaaaaacatc aaacggctta catttgaaga cttagtcgta ttgttgccac
     7261 tagttcagaa aaaagaatgg tgggacagca tcgactcttg gagaaaagta tacagcacat
     7321 ggtgtaaagc ctacccagag cagcgtgaag aagtctttca attgtttgaa aaaaaggaag
     7381 atttttggct acgtcgaata gcaatcacgt tgcaattagg ttataaacag caaacagaca
     7441 caggactatt gatccgagca atagacgaag atcgtcaaac aaaggaattc tttatccaaa
     7501 aagcaattgg ttgggcatta cgcgattata gtaagaccga tgccgattgg gtaaaaagac
     7561 agctgacaac agatttgagt aagttagctg ttcgagaggc aagtaagtat ttataagaaa
     7621 gcgagacgac aagagatgag acgcaaatta tttgcttttg atattgatgg aacgttgtta
     7681 gattcaaaca aacaagcttt agatagcaca agagaagcat tggaaaaatt acgcaaagcc
     7741 ggtcatctgg tcacgattgc tacagggcgg agccgatttc atgcgcaaga gatcattcgc
     7801 gatcttgatt ttacgaacta cattttatgt aatggagcgg ccggtttttt agaccatgaa
     7861 caagtatata agaatttact agatcgcgac cagttggatg cgttcgttca agaagcgcat
     7921 catgagcaga tcgatacagc ttttgtcagc ttagacaata tcaagcgttc ttcttccaat
     7981 gatgtaacgg tgatggaaga agcgatgcat tctttcggtg ctgtcttgcc tgaattagat
     8041 cagttttttg cagaggaaca agaaatatac caagcgttag catttttcga tcaaacatat
     8101 gatgggaagt tctcttcata tgataaaatt cgatttgtcc gctggcatga gaacagtgta
     8161 gatgtcgtac ctaaaaatgg ttctaaagca gcgacaattc tgcacttagc agaacaagta
     8221 ggcatccctc atgaagacat catcagcttt ggcgatggac aaaacgaccg tgaaatgttg
     8281 cgcatgtcag gaattgggat agctatgggg aatgctgtgc cagaagtaca agcagaagct
     8341 aagatggtca ctgatacaaa cgatcaggat ggaatttgga aggcattgaa agagttaagc
     8401 gtgatttaaa agaggttgtg aaaaagctgt tctgcatcaa gtaataagcc cagccaaaca
     8461 aaaatagctc ctcctatttt tcgtttttct gggcttatta ccgcagatgc aagctttcga
     8521 acaccgttta aggaagaggt cgtgacagaa gtggtcagtt tcaagaaata agaagggatt
     8581 tacgaaaatt gcttctcaaa tttttgtaaa attacggctt atttctgaag aagtagatga
     8641 aacaacgttt attcgggaaa tgggactgtg acaagatgtt tgtcatagtc ccattttttt
     8701 attttatttt tcaaaactaa gaaataccag gaaagagttc cttttgtttg accttttctt
     8761 tcttattgaa aacaactata atatagagtg atgttaaaag agaagaggaa tttagtgtgc
     8821 gggtattgtt tattggtgat gtagtaggtt caatgggacg agagatgatt actgaatatc
     8881 ttcctagatt gaagaagaag taccgtccgc aagtaacgat tgtaaatggt gagaatgccg
     8941 cttctggacg tggaatcaca gaaaaaatct ataaaaaatt tttgcaagac ggcgtagatg
     9001 tcgtgacgat ggggaatcat acgtgggaca accgcggaat ctttgagttt gtaaatgaag
     9061 ctaaaaagat ggttcgtccg gcaaattttc cggaagggac gcctggacaa gggatggtct
     9121 ttgtcaaagt gaaccaagtc gaactggcag tgatcaacat gcaggctcgt tcttttatgg
     9181 tggacttgga tgatcctttt cgtaaaatga atgagctggt agaagaagcc agaaaacgta
     9241 cgccgatcat ttttgtagat tttcatggag aaacaacaag tgaaaaacaa gcaatgggct
     9301 ggtttttaga tgggaaagtt tcagcagtgg tcggtacaca tacacacgtt cagacaaatg
     9361 atgcgcgaat tttgccaaga ggaacagcgt atctgacaga cgttgggatg acaggtccct
     9421 atgatggtat tttgggtatg cgcagagaac ctgtaatcga aaagtttctg actgcattac
     9481 caaaacgttt tgaagttgtg gaacaaggcc gatcgatttt aagcggctgt atcttggaat
     9541 tggatgatac gacaggtcat gcaaaagaaa tccagctgat tcagatcaac gatgatcgac
     9601 cgttcatgga gtagaaagag aggcagtttt ttgcagcagg agccggaaat tcaaaaagaa
     9661 gtaaggaaac tgacaaaact tttaagagag aatgaaacga tcattcgcta caaagaactt
     9721 gaagaaaaga tccagcaaaa ccagtattta gctgagttga gagaaaaaat caaacaagcg
     9781 caaaaagatg ctgttcattt tgcccactat gacaaaccag cagcagaaaa agaagcaatc
     9841 aaacaagcgg atcaattcat gcaagaattt gatcagcatc cgcttgttgt agcttataga
     9901 aaacagctgt tggaagcaga tgatctgctt catcatttaa caactatgat acaagaagaa
     9961 atcaatggac aaatagagga ggaaaaacat gcctcaaaaa actaaaaata ctcctatgat
    10021 ggaacaatat ctaagcatca aggcccaata ccaagatgct tttttgttct atcgtttagg
    10081 agacttttat gaactatttt acgaagatgc gatcaaagcg tcacagcttt tggaattaac
    10141 gttgacgagc agaaatcgaa atgcagaaga acctattcct atgtgcggtg tacctcacca
    10201 tgcagcacaa ggatatatcg atgccttgat tgaaaaaggc tataaagtag cgatttgcga
    10261 gcaggtagaa gatccaaaga cgactaaagg gatggtcaag cgcgaagtga tccagctagt
    10321 cactcctggt acagtcatgg acagcaaagg attgtcggca aaagacaaca attttttaac
    10381 agctgtagtt agtcaaggaa atgaatttgg ctttgcttat gccgatttaa gtacgggaga
    10441 attgaagacg gctcggttgc atgatgaaga agctgtttta aatgaagcct ctgcattgca
    10501 gacaaaagaa ctcgtattag gcagtgagat cactgatacc cttcgtgaac agctaagcgg
    10561 gcgtttaggg ctagtctttt ctcaacaaaa tgagatggaa gaaaacgcgg aatttagctt
    10621 tttgacaagc gaactagtcg atccgctgga aaaagaagca accggcaaac tattgactta
    10681 tttatcggtg acacaaaagc ggagtttagc tcatatccaa aaagcagtag aataccaacc
    10741 agatcatttt ttgaaaatgg attattattc aaaattcaac ctagaattaa gccaatctat
    10801 tcggactggg aaaaagcacg gcactcttct atggttgtta gatgaaacaa aaacagcaat
    10861 gggtggccgt ttactaaaac aatggctaga ccgtccgctt atccaggcaa aacaaattaa
    10921 agcaagacaa gaaatggtcg cttctttgct agacagctat tttgaacgta tcgatttgca
    10981 gtcagcatta acgaaagtct atgatctgga acgcctagca ggtcgagtag ctttcggtaa
    11041 tgtcaatggg cgagacttga tccagctgaa aacttcttta gaacaagtgc caatgatcag
    11101 agggttgatt gaaggaatcg atcaaggtca atggcaaagt ctgctttcag aaatgcagcc
    11161 gatggaccat ctcgtccaat tgatcgacca agctatccaa gatgatccgc cgttacaaat
    11221 aacggaagga aacatcatca aagatggatt caatgagcag ctcgatatgt accgttctgc
    11281 tatgagaaac ggaaagaaat ggttggcaga acttgaagca aaagagcgtc aagaaacagg
    11341 aatcaagaac ttaaaaatcg gatttaaccg cgttttcggt tattatatag aaattacaaa
    11401 agcaaatctt ggaaatgcag aactggagaa gtatgaacga aaacaaactt tagcaaatgc
    11461 agagcgtttc atcacaccag aactaaaaga attggagaca caaatcctcg aagcagaaga
    11521 aaaatctgtt gatttagaat atcagttgtt tttagctgtg agagaagaag tcaaaaaggc
    11581 tatccagccg ctacaagtac ttgcaaaagc aatcagtgca gtagatgtgc tacaaagttt
    11641 tgcaacgatc agcgaacgct atcagtatgt tcggccagaa cttgtcagtg acaagcatca
    11701 gcttctgatc aaagacggac gacatcctgt agtagaaaaa gtattaggac atcaggaata
    11761 tatccctaac agcgtagaaa tggcagaaga cgaaatgatc ttgctgatta caggaccgaa
    11821 tatgtctggt aaaagtacgt atatgcgaca gttggcatta actgttctga tggcgcaaat
    11881 gggctgtttc gttccggcaa aacaagcagt tttacctatt tttgatcgga tttttacccg
    11941 aattggtgca agtgacgact taattgctgg acaaagtact tttatggtag agatgatgga
    12001 agccaatcaa gccctgcgtt atgctacgcc aaacagtttg attttgtttg acgaactagg
    12061 gcgaggaaca gcaacctatg acggaatggc gttggcccaa gcaatcatcg aatatatcca
    12121 tcgtcatgta caagcaaaaa cactcttttc tactcattat catgaactga cggtacttga
    12181 gaaagaactt ccgcagctta aaaatgtcca tgttggagcc gttgaaaaag acggagaagt
    12241 tgtcttcttg cataaaatga tggatggacc tgcggataaa agctacggca tacatgtggc
    12301 aaagatcgca ggattgcctt ccaatctact agaacgagca gcagatatcc tgcatacgct
    12361 agaaaatggc gataacggac accatactgt tccagctgaa gtagaagaga aaactaaaac
    12421 agccaaacag gagcaaataa aaccacctgc agaaaaagta aatcaagacc atatacaaga
    12481 ccgtgtaaaa gaagaaaacc aacagctttc tttattcagt gaattatccg aaaatgaaac
    12541 agaagtcatt gaatcactga agcaaataaa tttactagaa atgacgccaa tggatgcact
    12601 aaacacatta tatgaattac aaaaacaact gtaggaagaa ggcggatatc gatggccaaa
    12661 atccaagaat tatctgaacg tttagctaat cagatcgctg cgggagaagt agtggagcga
    12721 ccagcttccg ttgtgaaaga attagtggaa aatgcaattg acgccggcag tactcagatt
    12781 gatatcttag ttgaagaagc gggactgcgg acgatccaag tgattgataa cggagaaggc
    12841 attttggcag acgacgtaga gaatgccttc aagcggcatg cgacaagtaa gatccataat
    12901 cgcgacgatc tttttcgcat acgaacgctg ggctttcgag gggaagcttt gccaagtatt
    12961 gcctctgttt ctgaaatgac tctggaaacg gcgaatcagg aagaaaaaca aggcacctat
    13021 ctgtcgctga aaggcggaga agttattgag catcgaccag caccactgcg tcaaggaacg
    13081 aagattaccg tcagcaatct gtttttcaat acgccagccc gcttgaaata cgtgaaaaca
    13141 ctgcacacag aattagcgaa catcggggac atcgtcaatc gactggcatt gagtcatcca
    13201 tcgattgctt ttcgtttagt acatgatgga aacaaaatgc tggcaactgc tggaaatggc
    13261 gatctgaaac aaacaattgc gggaatctac ggattatcta ccgcaaaaaa aatgctgaaa
    13321 atcgaaggaa aagatctaga ttttgagtta tttggctatg tttcattgcc tgaggtgact
    13381 cgtgccagta ggaattactt atcgacgatc atcaatggac gttttatcaa gaacttttcc
    13441 ttgaataaag caatcgttgc aggttatggg tcaaaattaa tggtcggtcg tttccctatt
    13501 gctgtattgg aaatcaaaat ggacccgctt ttagtagatg taaatgttca tccgacgaag
    13561 caggaagtcc ggctgtcgaa ggaaaaagaa ctaacagagc taattagtaa cgccattcaa
    13621 aaagcattgt ctcaagaaaa tctgattcca agtgcagcgg ataatctacg gtttaagaaa
    13681 aaagtacctg atcagcctaa aacagaacaa atcgaaatcg atttgtccta cgcttttgaa
    13741 gaagaaaaac ctaaaaagcc aagtagtttg aattttgatc gagagacagg tacctttttc
    13801 gtagaagaac taaagaacac atttgttgac agaattgttg ataagtctgt ggaaaatgtt
    13861 gaaaactatt ctggtttttc tgaagaaaac accacttatc cagaaaaaac agcgaaaacg
    13921 gtggataagt ttggtgaaaa tgttgataac tttgtagaaa aaacagtgga aaaatctgaa
    13981 aagaaagaat ttcccattgt ggataacatg gagaaaacaa atgaagaaca agcagataaa
    14041 tccgtagaag aggatgaaaa gatttacgag gaagaattga cactccatcc tgaatttgat
    14101 ttcactcaag gaaacgacaa aacattagaa caagccatca agaaattaga aacggaaaaa
    14161 ccagtacaac gttttcctca gttggaatat tttggacaga tgcacggaac gtatcttttt
    14221 gcccaaagta gcgatgggtt gtacatcatt gatcagcatg ctgcccaaga gcgcattaag
    14281 tatgaatatt tccgggaaaa aattggggaa gtatctgatg atctgcaaga attgcttgtt
    14341 ccatttgtgt tagactatcc aaacagtgat gctttgaaat taaaagagca aaaacacttg
    14401 ctagaagatg cagggattta tttagaagaa tttggtcaaa acagctttat tgtacgggcc
    14461 catcctactt ggtatcccaa tggagaagaa gaatcgatta ttcgggagat gatcgatatg
    14521 ctgctgacaa ctggaagtgt gagtgtaaaa aaattccgtg aagcaacagc aatcatgatg
    14581 agttgcaaac gatcgattaa agccaaccat tatttgaatg aagcacaagc acgtgtgctg
    14641 ctgaaggatc tggcacgatg cgaaaatcct ttcaattgtc cccatggccg tccagtattg
    14701 attcacttta ccaattcgga tatggaaagg atgttcaaac gaatccaaga cccgcattaa
    14761 gtcaaagaga agagggaaaa catgaagatt gtattagcat cccaatcacc aagaagaaaa
    14821 gagttattgg ctcatatcgt ttcagaattc gagattgaac ctgctgatat cgatgaaacg
    14881 ccatttccag acgaacagcc cgtcgattat gttcgtagaa tggctgaaga aaaagcacga
    14941 actgtctggg aacaagatga aaaagaagac catttagtga ttgccagtga cacgactgtt
    15001 gttctcaatc aagagatcat gggtaaacca gaagatctgg ctgaggctga attcatgctg
    15061 aaaaaactaa gcggggaaac acatactgta tatacagctg ttgttttaaa aacaaaggaa
    15121 cgagaagaac ggatactagc agaagcacat gtcacttttt atcctttgac agacgaagag
    15181 atcaaacgct acttggagac gggggattat gcggataagg caggggcata cggcatccaa
    15241 aatgctgcga gtgtattcgt caaagaaatt tctggagatt attacagtat cgtaggattt
    15301 ccgattggtg ctgtcaatca ggcactgaaa acattcattt aaacaaaaaa gcggactatc
    15361 tgattttcga ttgaaaatcc atgatagccc gtttttttta ttgaaaagat tacttatcta
    15421 ctctgactaa gattttactt cagctttacc gactgcttcg cccgggcgaa caagtcctga
    15481 ttggtcaaga gaaagtcctg caactttttc attattggtc aaagcaacaa taatgtcagt
    15541 tttcttgcca gcttgttcta tttctggcaa atccatttca gcaatcaggg tctctggtgt
    15601 gacaaggtat ccttctttca cgaatacatt gaaaggaaca ccctttagtt caacagtatc
    15661 taatcccatg tgaacaagta cttccagccc ctcattagaa agaataccaa tggcatgttt
    15721 tgtttcaaag acactcgtca ctttaccgtt tactggtgca tagactttgc cgtttgacgg
    15781 ttcgacagca taaccgtcac ccatcatttt ttgtgagaaa actgggtcat ttacttcagt
    15841 tatttccttg atggtccctg cagcgactgc gaataatgtt tcttttgttg caactgccgt
    15901 accagttgct gaaacaggtt cgaaagactg tggcgttgct gttgcgccat ttgctgtttg
    15961 aagttgtaca tcagcagcat tttcaggaac agcaggatca atcttattca aaattccctg
    16021 acgtctaaag atgatcgtca agccaaatgg aatgatgaca gcaatgatca ttgcgatgat
    16081 gaaaggtacc catgtttctg cttggatagc tagtattcct ggcaaaccgc ctactccgat
    16141 cgcgttagct cgaacaccca tcaagttagc aaacatgcca gctaatccag agccgaccat
    16201 tgcagcgacg aaaggataga cgtatttcaa attgattccg aacatagcag gttcagttac
    16261 gcctaagtaa caagaaatca ttgctggaat cgagatttgt tcttcttttt tattcccacg
    16321 atgtaagaag atgatggcta agactgcaga gccttgggcg atatttgata aagcaatcat
    16381 cggccataaa ttcgttgatc cgaaatctgc aatcagctgc atatcgatgg cattggtcat
    16441 atggtggaga ccagtaatca ctaaaggagc atacaggaat ccgaatacgg cactaaacaa
    16501 ccagctgata gaagacgtca atccagtatt tacgatattt gagatccagc ttccgactgt
    16561 ccagccgata ggacctaaaa taacgtgtgc agctaaaaca gttggtaaca atgaaaacaa
    16621 tggaacaaaa atcatcgaga tcgactgagg gatatatttg cggaagaaga tttctagata
    16681 cgctaacata aaccctgcca gcatggctgg aataacttgc gcttgataac caatcatctg
    16741 gacttgagca aaaccaaaat cccaaaatgg aatatctgct gcggctgtgg aagctacact
    16801 ataagcattt aataattgcg gagaaactaa cgtaattcct aaaacgattc ctaatatttg
    16861 tgtagtaccc atcttttttg caatactcca agtgatacct actggaagaa aatggaaaat
    16921 tgcttcaccc ggtaaccaca aaaaggcatt gacaccattc caaaacttcg agtgttcaac
    16981 gattgttcca ccaagacttt caaactggat accttcaagt acatttctga atccaaggat
    17041 caatccaccg acaatgattg caggaatcag tggtgtaaag atttctgcta aaacggcaat
    17101 agcacgttga acgggatgaa ggttttgttt tgcagctgct tttccttgtt ctttggaaac
    17161 cccttcaact ccagatactg caacgaagtc gttataaaaa gtagaaacat cgtttcctat
    17221 gatgacttgg aactgtcctg cattcgtgaa catccctttt acgctaggta tatcttcgat
    17281 cgctttttcg tccgcttttc ctggatcatt caggacaaaa cgcatacgag tcgcacaatg
    17341 actaactgca gcaatatttt cttttccgcc gatatcatgt aacagcttct cggcatctgc
    17401 ttgatatttt gccatatcaa taaacccctt tctcttaact tgtctatgca agttgtttct
    17461 tcatttacta tacttttttt cggagagaat tgcaagcgtt ttattaaaaa gtacgagaga
    17521 aaatgatatc gttttaaaaa gcttaaaata agcttttctt aaaaatagga acattgaaat
    17581 acaaaaagag ctgtagtata gtgaagaaat gatggaagaa cgagtgaggg aaaaaacatg
    17641 aataagttca atgaaatttt tttagatttg gaacagaaaa ttttagcgaa agaatatcct
    17701 ccgcattcgc ttttacccag tgagaaccaa ttgatcaaaa tttatggtgt ttcacgagaa
    17761 acaattagaa aagcgctgaa tcttttgact acggaaggtt atattcaaaa aaaacaagga
    17821 aaaggttcga tcgtactgga tcggaatcgc ttcgactttc ctatttctgg attgactagc
    17881 tataaagagc tacaggagac ccaacgtatt ccaagtgaaa cgatcatcca tacgttagaa
    17941 gaaacagaag tgacaaaagc attgaacgag atcactggct gggagatcgg tgcgcctgtt
    18001 tggcatttga tacgcgaaag aaaaatcaat ggcgaagtag ttatattaga caccgattat
    18061 ctattaaagg aaatcgttcc tattttaact ccagcacaag cacaaggatc gatctatgaa
    18121 tattttgaaa atgagctttc tttgacgatt gcttacgcgc aaaaggagat cacagtagaa
    18181 gaagtaacgg aaatcatgaa aacagcgatg gatcttcacg atgacactta tgcagtagta
    18241 gtcagaagcc tcgttcatct ggaagatacc cgctgtttcg agtattctga gtcggttcac
    18301 cgattagata aattccgttt cgttgagttt gcccgacgcc gaaaagtgta aatagttcaa
    18361 aagaaagcaa attgttggca tctgtccact ttggcgctat gataggatca caaagggggc
    18421 atttttaatg gataaaccaa taagaccaga aaaagaagaa ctgaaagaaa agttatcacc
    18481 catggcttat gcagtcacac aagaaaatgc aacagaacga ccatttagcg ggaaatatga
    18541 tgatttttac gaaaagggga tctatgtaga tatcgtcagc ggtgaaccgt tattcagctc
    18601 tgcagaaaaa tatgatgcag gttgtggctg gccatcattc agcaaaccaa taacgaaaag
    18661 acaagtgcgc gaaaaagccg atttttctca tggcatgcat cgaatagaag ttcgaagcaa
    18721 agaagcagat tctcatctcg gacatgtgtt taccgatgga ccagtagatc aaggtggtct
    18781 acgttactgc atcaattctg cctcactgaa attcattcca tatgaaaaga tggatgaatt
    18841 aggttatggg gaattcaaat cacttgtaga ataatggaaa tacacaaaga gcggtacaat
    18901 acggggattt actcgatcgt ccgctctttt tttattcaag cgattgctat ttgaaagaaa
    18961 cgctccctag actccttttt tcacaaggtg aaatgtgcta caataaaggg aacgtatgtg
    19021 tgcaaaagaa agaggactag aatatgtatg aatatctgac aggtgtggtt acctttatca
    19081 acccctatta catagtagta gaaaccaatg gtatcggcta ccagatcgca ctaggaaatc
    19141 cttaccgcta cagtagcaaa ctaaatcaaa aaataaaaat ttatgtccat caggtaatac
    19201 gtgaagatgc gcatcttctg tatggatttg attcgctaga agaaaagcaa ctgtttttgc
    19261 gattagtaag tgtttcagga atcggtccga aaagtgccct tgcaatcatg gcaagcgaag
    19321 accactccgg tttgattcaa gcagtagaat ccagtgatgt cacttatttg acgaaatttc
    19381 caggagtagg gaaaaagaca gcacaacaaa tgatcttgga tctaaaagga aaattcggtg
    19441 agctgagtat cgatacacct tttagtctat ttgatgaagc aaacacaaaa gacgctacag
    19501 ctctatctga agcaatagaa gctctctcgg ctcttggtta cagtgatcga gaaatcaaac
    19561 gtgtagaaaa acaattaaaa gaagtagata atcagacaac ggatgagtat ctacgccaag
    19621 cactcaagct aatgatgaaa aaataaggag gagaccaaat gacagatgaa tacttgttat
    19681 caccagaatc tggcgaaaat gaactgagtt tggaaaaatc tcttcgacca cagctgcttt
    19741 ctcaatatat cggacagaga aaagtcaaac aagaacttca gatttacatc gaagctgcca
    19801 gaaatcggga agaaccttta gatcatgttc tgttgtacgg gcctcctgga ttaggaaaaa
    19861 cgacgatggc catggtcatt gctaatgaga tgaccgtaaa tattcgtacg acgagtggcc
    19921 ctgctatcga acgtccgggt gacttagtag caatcttgaa tgaactggaa gcaggggatg
    19981 tcctgtttat cgatgagatc catcgattac ctagagtggt agaagagatg ctttattcag
    20041 ctatggaaga tttctatgta gatatcatgg taggacaagg gccaaccgca catccggttc
    20101 atttccctct tcctcccttt actttgattg gcgcgacgac acgggcaggt atgctttctg
    20161 cacctttacg agatcgattt ggcatcattt ctcatatgga atattatgac gaacaggatc
    20221 tgcaggagat cgttttacgt tcttcggata tttttcaaac agagatcatt gaagaagggg
    20281 cattggaaat tgctcgccga tcacgaggaa cgcctagaat cgctaatcgc ttgctcaaac
    20341 gggttcgtga ttttgcgcaa gtccaaggag acgggaaaat cgatcgtcct atagcggatc
    20401 aggcacttac tttgctacag gtcgatcagg ctggattgga ttatgttgac cagaaattgt
    20461 tgcgaacaat gatcgagtta tatggcggcg gaccagtagg gttaagtacg ctttcagtaa
    20521 acataggaga agagcgtgag acggtagaag atatgtatga accgtatctg atccaaaaag
    20581 gatttttaaa gcgtacacct agagggcgaa ttgctactgc ttatgcctat gaacattttg
    20641 gttacgacta tcatatatga tttttgcttt tagattggag gaaaatgaat gagtgataca
    20701 acacgtacga aacaattgat caaaaacgta ttgattgatt tgtgccaaac gcaatctttt
    20761 cgaaaaatca gcgtccaaaa aatcgcaaac gaagcaaaaa tcaatcgaca aacattttat
    20821 tatcatttta cagataaata cgatttgctt cgttgggtct attgtgagga tgcattgcat
    20881 tatttagatc cacaatgtct ttcacttgat aattgggaag agcaagcatt gaaaatgctg
    20941 aaagcaattg ctgagaagaa agaattttat gcaaatactg tgctttctga cccagaaatt
    21001 ttacaaaaag agttttctca actgatccaa aaatcattta tcaagatttt tgaacaaatg
    21061 gatgaagaaa gacaactttc tgcaagagac aaactatttt atgctcgttt tttttcttac
    21121 ggttgcagtg gtgtattagt caattggatc acgaatgaat atgaagaaac accgatggaa
    21181 atcgctatgc agcttttccg tttagccaag gatacggaat tatattctta tcgtctttat
    21241 gcaaaagaag agttggaaga acagtaatag aaaaattttt tgttggaaaa gcaataatcg
    21301 ctaaaaggag atggggaaat gacaaaaatt ttatttgttt gtttgggaaa tatttgccgt
    21361 tcaccgatgg cagaaggctt gctgagaaaa cgaatagcat tagaaggaag ggaagcagaa
    21421 ttttttgtgg attctgctgc tacaagcacg tatgaagttg gaaagacacc gcaccctgga
    21481 acgaaaaaaa tattaaacca agaaaaagta gatatgacga atatggttgc tcggcaaatc
    21541 acaccccacg attttgaaac ttttgattgg atcattggca tggatcaaga aaatgtagaa
    21601 gaactgaaac gaagagcacc acgctcagcg caggggaaaa tccatttgtt tttaagtagc
    21661 attcctggaa aagaaaaaga aaatgttccc gacccatact ataccaataa ctttgatgag
    21721 acatatcact tgatcacaga aggcctgaat cattggtgga atatttggat tgatgaaaat
    21781 aaaataacaa ataatttgta aagttaacgc tttcttttta aggcgatgtt agagcatttc
    21841 cttgtttttt tgtaaaatgt taggtaaaat aaaattaatt aagtaacact ttcttagcta
    21901 aaggaggagt gcattcgatg aacgaagaga cagtatcgcc aatgaagaag cttacgaagt
    21961 tagttactca tacttcgtat caactttttg attcttttgt aaatgacact gaagaacatg
    22021 ctaagaatgt acgattgcaa ggaaagttta ttcttgagct gcaaaaagct gctactgaac
    22081 atagtctagt tatcttacaa gtaagagcaa atcgtttgag tagcaagtat gaaacgatct
    22141 caggctgggt ggcgacaaaa acggtcgaaa aagaccgagt gatgatccgc ctacaaaacg
    22201 aagagcagca gatccggatg gttcctgtag aacaaattga aaaaataaca atcctttcac
    22261 cagcagggga acaggaacgg ttatcaaaat aatttatacc agatagaaaa gcacgtatga
    22321 gcttgtacca tcaatcatta ctaatcaaaa ataaccgaat gctttaagct gatatcagta
    22381 aaacggaacg ttttaggatg agtatcatag catgacagca ttcggttgat
//
LOCUS       DACDKL010000026        21902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDKL010000026 DACDKL010000000
VERSION     DACDKL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.026"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(449..1078)
                     /gene="udk"
                     /locus_tag="IV329_000793"
     CDS             complement(449..1078)
                     /gene="udk"
                     /locus_tag="IV329_000793"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8939997.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1162..1857)
                     /locus_tag="IV329_000794"
     CDS             complement(1162..1857)
                     /locus_tag="IV329_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8939998.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1870..2586)
                     /locus_tag="IV329_000795"
     CDS             complement(1870..2586)
                     /locus_tag="IV329_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8939999.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2916..3524
                     /locus_tag="IV329_000796"
     CDS             2916..3524
                     /locus_tag="IV329_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8940000.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3849..4172
                     /locus_tag="IV329_000797"
     CDS             3849..4172
                     /locus_tag="IV329_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8940001.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4354..5724
                     /locus_tag="IV329_000798"
     CDS             4354..5724
                     /locus_tag="IV329_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8940002.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5854..7155)
                     /locus_tag="IV329_000799"
     CDS             complement(5854..7155)
                     /locus_tag="IV329_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8940003.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7509..9500)
                     /locus_tag="IV329_000800"
     CDS             complement(7509..9500)
                     /locus_tag="IV329_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8940004.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9644..9910)
                     /locus_tag="IV329_000801"
     CDS             complement(9644..9910)
                     /locus_tag="IV329_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8940005.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10420..12546)
                     /gene="pnp"
                     /locus_tag="IV329_000802"
     CDS             complement(10420..12546)
                     /gene="pnp"
                     /locus_tag="IV329_000802"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8940006.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12734..13003)
                     /gene="rpsO"
                     /locus_tag="IV329_000803"
     CDS             complement(12734..13003)
                     /gene="rpsO"
                     /locus_tag="IV329_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8940007.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13254..13817
                     /locus_tag="IV329_000804"
     CDS             13254..13817
                     /locus_tag="IV329_000804"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8940008.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14070..14591)
                     /locus_tag="IV329_000805"
     CDS             complement(14070..14591)
                     /locus_tag="IV329_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8940009.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14594..15436)
                     /locus_tag="IV329_000806"
     CDS             complement(14594..15436)
                     /locus_tag="IV329_000806"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8940010.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15429..16154)
                     /locus_tag="IV329_000807"
     CDS             complement(15429..16154)
                     /locus_tag="IV329_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8940011.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16330..16626
                     /locus_tag="IV329_000808"
     CDS             16330..16626
                     /locus_tag="IV329_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940012.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17194..18786
                     /locus_tag="IV329_000809"
     CDS             17194..18786
                     /locus_tag="IV329_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8940013.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18799..19539
                     /locus_tag="IV329_000810"
     CDS             18799..19539
                     /locus_tag="IV329_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8940014.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19707..21056
                     /locus_tag="IV329_000811"
     CDS             19707..21056
                     /locus_tag="IV329_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8940015.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21184..21774)
                     /locus_tag="IV329_000812"
     CDS             complement(21184..21774)
                     /locus_tag="IV329_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8940016.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4461 c         4151 g         6996 t
ORIGIN      
        1 caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga aataattttc
       61 gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc gttaaacggt
      121 gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg aggagctgtt
      181 ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct ctacttcacg
      241 gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata aacagctatt
      301 ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct cttgttataa
      361 ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt tcaaacggtc
      421 gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta gctacttttg
      481 ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg atatccgcat
      541 agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc aaatattgtt
      601 cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata cgacgaatga
      661 tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca cgcagacgtt
      721 cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc tgaatgatcg
      781 ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg gctttatagt
      841 tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat gggtgatcgt
      901 aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa taagaatctt
      961 gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg ctgacacttg
     1021 tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt tttgtcattg
     1081 actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa aaaacacgaa
     1141 aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat aaagttcctc
     1201 taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt tatttctatt
     1261 gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag tcaaagaatc
     1321 accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga catgatcaaa
     1381 aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat ccttgaagta
     1441 atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac cgttcgtcac
     1501 aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc ctggaacaag
     1561 atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac tatctactgt
     1621 tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt ctcgtgatag
     1681 tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc tggtttcgat
     1741 ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat tggcttcagt
     1801 taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat atttcatctt
     1861 tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg tcaacgatcg
     1921 atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta gccgtttcaa
     1981 tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga attttccttc
     2041 ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt ttaaatcagc
     2101 cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg ttacgtctgc
     2161 cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt tttctggaag
     2221 tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg ttgtatctct
     2281 atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa tcagatcgat
     2341 tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt tattgagaag
     2401 ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat gcagtaaact
     2461 atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt gcttcaaagc
     2521 aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta atttatctaa
     2581 acgcatgctg tttcctttct tattactact aattaattat accttgaata ttcttgcttg
     2641 caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac ggttaaatag
     2701 taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa gttgaatcct
     2761 gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc gccctgcctt
     2821 cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt ccgttaccaa
     2881 agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta gaagtaagta
     2941 aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg aaagttccag
     3001 ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt ttgacactaa
     3061 gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc gatggacaaa
     3121 aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat ttcgaacatg
     3181 cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa gtcgttcttg
     3241 ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg tatacagtag
     3301 tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg acaaacttgg
     3361 aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac aaattcgtga
     3421 ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa gaaagcacag
     3481 gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga aacaatcagg
     3541 tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc taatcgttta
     3601 ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc ctaatttacc
     3661 aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt gaaaaaaagt
     3721 attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg ttgaagtaag
     3781 tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat tcgaaggagg
     3841 ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca gttggtgtcg
     3901 tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac ctagatctta
     3961 agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat cgtggaaaag
     4021 tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat ttgccgtttg
     4081 cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg agagaaacga
     4141 tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg atgtccctta
     4201 ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc tttttatgga
     4261 gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct atatactact
     4321 aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa aggaagcccc
     4381 gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt tcagcagttc
     4441 tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc gctggcagcg
     4501 gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg tttaacagct
     4561 ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg tctgaagaaa
     4621 agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac tgtcctaagt
     4681 ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat acttgatcaa
     4741 gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag tttcaatttg
     4801 ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt tctgatcatt
     4861 gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg catgggtgtg
     4921 gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact gtgtctagtg
     4981 tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa atttgaaaaa
     5041 gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc ttcgatcatc
     5101 gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga tgtcgtagct
     5161 gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat gtcatttggt
     5221 attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg ccgaatcttg
     5281 caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc aggtgctatc
     5341 gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga aacacgtgta
     5401 tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat cttagcagtc
     5461 ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc tacgatcgcc
     5521 ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg aatgtgggga
     5581 tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc cgtgttgctt
     5641 tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga acatttcaca
     5701 ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc taaaaatgaa
     5761 aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga taacaaaaag
     5821 gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt ccgtggactc
     5881 tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac ggaatgatgc
     5941 aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa agcccgcaag
     6001 tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac gctggaaatc
     6061 atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg ctatcaatgc
     6121 ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa tatcttcaat
     6181 cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat gcttaccctt
     6241 ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta cgatcggctc
     6301 ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga tcaaatctgg
     6361 caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat agcgagtatg
     6421 tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt cgaatacttg
     6481 catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt caaacacgtt
     6541 ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat tcaatgcgac
     6601 taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac gcacacctaa
     6661 tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa acattacttt
     6721 ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat tgacgatact
     6781 atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta aaataaatcc
     6841 cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac cgatcactgg
     6901 ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac cggttagcgt
     6961 aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat gaagagctag
     7021 cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc tgattttcac
     7081 taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa ataaaataat
     7141 gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt tttctacaaa
     7201 aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg gcatttttca
     7261 gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca gctttttcat
     7321 gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc caaacagatg
     7381 aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag tacagctttt
     7441 tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt caggtcaaag
     7501 cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat gataagcgga
     7561 acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg cgaggtagcg
     7621 atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc tagagttgtc
     7681 acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc tcgccctggt
     7741 gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa atcatggatg
     7801 acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa cagattcact
     7861 tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata tggctcatcc
     7921 gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt atctaaaatc
     7981 gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa gaagaccaca
     8041 tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg cgggatataa
     8101 tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg ttcccaaata
     8161 aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc tcggaagaat
     8221 ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa ggtaactaaa
     8281 tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag tgctgttgtc
     8341 atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg agacgttcgg
     8401 aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc tgggatatac
     8461 atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag tttcaacttg
     8521 attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc aatgactgca
     8581 gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat ttggaagaat
     8641 ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa caaccacgca
     8701 gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct cagacgaata
     8761 tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt cgtagccagg
     8821 aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag cagctggata
     8881 gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt cattaatccc
     8941 attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt tccgacaata
     9001 tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt gataatgacg
     9061 atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg caagccttca
     9121 atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa ggcagctcct
     9181 ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt gtagagtgag
     9241 aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata tttgattgtt
     9301 gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa atcaggattg
     9361 attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag cggacttgtc
     9421 ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga tatctttttg
     9481 gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata tcctttaggt
     9541 aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa ttgcttctaa
     9601 ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct ttctttggac
     9661 gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt gcacccaata
     9721 atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt aaaaattcgc
     9781 cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc gggacttcct
     9841 ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac gaccataaaa
     9901 aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt acgctttatt
     9961 cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt gtaacaaaag
    10021 tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag gttgtgaaat
    10081 cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg
    10141 ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag aggttgtaaa
    10201 aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct aatgttttta
    10261 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa
    10321 ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg tccccctgtc
    10381 accttcacga tcacaagtac attattccta tgaattaaat tatttttctt catttttcgt
    10441 tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt gtttatcgat
    10501 ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga ctttattgac
    10561 acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga tcaagttgac
    10621 gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac caactttgac
    10681 ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg catcagctga
    10741 agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt catcgatgat
    10801 tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag gttcgatctg
    10861 gatcatttcg atttttggtg catattggct taattcttca cgtggagcag caatcgttga
    10921 agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg cttcagttag
    10981 gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga taccatcttt
    11041 tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt ggatatcagt
    11101 taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg cgactggcgc
    11161 tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac ttgcttggga
    11221 agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag gaaattcttc
    11281 ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc cgatttcccg
    11341 acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga aattgtagtg
    11401 gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt gctctcctag
    11461 aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca aacctgatcc
    11521 atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat ccaatttacg
    11581 cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt tttcaagatc
    11641 ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac ctgcaaattt
    11701 ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt cgcgagcaag
    11761 tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa agatttcttt
    11821 tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc ctactgcttg
    11881 aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa acagcaatgc
    11941 accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat tgatggcttg
    12001 tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta ctgttggatt
    12061 caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc cgtcaaatgg
    12121 gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag ctggcgagca
    12181 gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga agccttctgc
    12241 aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt ccgtacttgg
    12301 acggccttct cgtttgatga aacctccagg gattttccct gctgcataca ttttttcttc
    12361 ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg ccacagctgc
    12421 acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt gttttgctag
    12481 ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata cttgtttttc
    12541 tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca aatgaaaact
    12601 gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc tagcttgatc
    12661 acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg aatcccactc
    12721 tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg taacgttgaa
    12781 cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt ttcattaggc
    12841 cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag tggttgattt
    12901 cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca tggcgagcgt
    12961 attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc acctctttcg
    13021 attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg gcaggtcatg
    13081 tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag tagttaatca
    13141 ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg tggtaaacta
    13201 taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat tccatgatta
    13261 caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca aaagaagttt
    13321 ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag ttcttaaaaa
    13381 acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt ggcttagctg
    13441 ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt cctgacccag
    13501 aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg agccattctg
    13561 ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga gaagtaccag
    13621 gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat ggtgaaaaac
    13681 acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc gaccatatca
    13741 acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta aaagaaggcg
    13801 tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca agaaagcttt
    13861 ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc cactaatcga
    13921 aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg gatacgcatc
    13981 ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg atagaatagt
    14041 ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc tgcttcagct
    14101 agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt atgcagatgg
    14161 atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg tctcataaag
    14221 gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc gtaaagggga
    14281 tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct cagttcttct
    14341 tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt attggtttct
    14401 tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc agctcgtagt
    14461 aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc aaacttgcta
    14521 gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg ccgtgtctct
    14581 ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag tgtttcatca
    14641 aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc agactaacaa
    14701 ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt tcttcttttt
    14761 tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca accaactctc
    14821 gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt agttcttcct
    14881 ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg ggaaaagatt
    14941 tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca cccggcgtga
    15001 cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt gcgtactcca
    15061 gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc tggacattcg
    15121 gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat gtacaattga
    15181 aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa ttgataactg
    15241 gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat tgggatcgca
    15301 gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac atacggtcgg
    15361 caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt tctttttcgg
    15421 ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac aaccgctccg
    15481 tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt gattactaag
    15541 attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac ttgaaaactc
    15601 gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg ctccatgact
    15661 ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc tccgcatgct
    15721 tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca ttttcgacaa
    15781 tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc ctttgcgtgc
    15841 aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag atgtgctatt
    15901 tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac aaattgatag
    15961 aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg ctgctccatc
    16021 ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt ggatacccct
    16081 gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc ttcgtctact
    16141 gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc gaacaaattt
    16201 attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg agcaatttat
    16261 cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat aaaagtgagg
    16321 tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg tatcgcctgg
    16381 ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa tggaaatctt
    16441 tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac attagccgta
    16501 tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct aatattagcc
    16561 atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa gaaagcggca
    16621 gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt ctttttttat
    16681 tgtattacat acatttttgt gtataattat actatcgttt gcaatggata gatgattcac
    16741 attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat tttttcaaaa
    16801 accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt gtataaataa
    16861 ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta accctgtgga
    16921 gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca cttttttgct
    16981 tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag tgatgctaag
    17041 ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc tttttatatc
    17101 tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca tctagaccgc
    17161 tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt ttcagttgcc
    17221 gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa tgcagatgaa
    17281 aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc cttcaactgg
    17341 tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag ttacgccggc
    17401 ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa attagtcatc
    17461 gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat cgacgcgatc
    17521 atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac cgatccttat
    17581 tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa agcaaaaagt
    17641 ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca ctatactgtg
    17701 atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc agcaatgaga
    17761 acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga aggtgtaacg
    17821 gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga tggcttccag
    17881 acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc ggatatccaa
    17941 aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat tatggaccaa
    18001 gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat catttctgat
    18061 ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg acttaccttg
    18121 ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat tggtgtcttc
    18181 cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat cggaaatgca
    18241 cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca agcaatggtc
    18301 atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat tgccgctttg
    18361 tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg ggggattttt
    18421 gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca tggccaaacg
    18481 atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac aggaaatgag
    18541 tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc tgatttgttc
    18601 ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt tacgatcgtc
    18661 gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc ggtagaaaag
    18721 aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc aaatttgcag
    18781 gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct aagaaaaagc
    18841 ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg tgaagttgtg
    18901 acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat caatttactg
    18961 gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc tcctaaatat
    19021 aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa cctgttcaac
    19081 aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa acgagataaa
    19141 gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga tcgttatatc
    19201 gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat tgctcgggca
    19261 ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt agaccctgaa
    19321 atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt gacaatgctt
    19381 atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat ttttatggat
    19441 aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc gaaagaagaa
    19501 cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct gattgggaca
    19561 aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag gtaaatttct
    19621 gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg aagtaattaa
    19681 acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag cgtggactga
    19741 aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg ttgtttttag
    19801 gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat cttattactg
    19861 gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca aacaccaata
    19921 accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct ttcatcactg
    19981 gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca gccatcggaa
    20041 tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg attgcattag
    20101 gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta gagttttggt
    20161 ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct tatatgatcc
    20221 tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg tctcatggcg
    20281 gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta acctttgctt
    20341 ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct gaaaaagtgt
    20401 tgccggaagc tatcaataat attcctatca ggatcatctt attttactta ggatctcttt
    20461 ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt ccatttgtag
    20521 aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta gtcgtattaa
    20581 gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg cttcgttctt
    20641 tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat catgtgccag
    20701 ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg aattacgtga
    20761 tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc ttgtttatct
    20821 ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc aaaaaagctc
    20881 attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt tacatggcct
    20941 ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta ctaacacctg
    21001 tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag aagtaagaaa
    21061 gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga aaattgcttc
    21121 ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt cccagcctct
    21181 tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat aggaaaaaat
    21241 ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa aaaagacacc
    21301 ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct ttgacaaaac
    21361 tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca agataccaaa
    21421 agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa aagcttcagg
    21481 gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga gcaaaaagat
    21541 attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac gatgatggtt
    21601 cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg tatttcggat
    21661 gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata caataactct
    21721 caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg tcacgatgat
    21781 tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat aaccatcgtt
    21841 ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt acttttaaaa
    21901 aa
//
LOCUS       DACDKL010000027        21862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDKL010000027 DACDKL010000000
VERSION     DACDKL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.027"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..378)
                     /locus_tag="IV329_000813"
     CDS             complement(<1..378)
                     /locus_tag="IV329_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8940017.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITFQEPLAIQH"
     gene            complement(455..985)
                     /locus_tag="IV329_000814"
     CDS             complement(455..985)
                     /locus_tag="IV329_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8940018.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(989..1849)
                     /gene="rlmB"
                     /locus_tag="IV329_000815"
     CDS             complement(989..1849)
                     /gene="rlmB"
                     /locus_tag="IV329_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8940019.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1842..2237)
                     /locus_tag="IV329_000816"
     CDS             complement(1842..2237)
                     /locus_tag="IV329_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8940020.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2234..3646)
                     /locus_tag="IV329_000817"
     CDS             complement(2234..3646)
                     /locus_tag="IV329_000817"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8940021.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3643..4179)
                     /gene="cysE"
                     /locus_tag="IV329_000818"
     CDS             complement(3643..4179)
                     /gene="cysE"
                     /locus_tag="IV329_000818"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8940022.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4501..5961)
                     /locus_tag="IV329_000819"
     CDS             complement(4501..5961)
                     /locus_tag="IV329_000819"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8940023.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6131..7288)
                     /locus_tag="IV329_000820"
     CDS             complement(6131..7288)
                     /locus_tag="IV329_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8940024.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7322..8692)
                     /gene="radA"
                     /locus_tag="IV329_000821"
     CDS             complement(7322..8692)
                     /gene="radA"
                     /locus_tag="IV329_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8940025.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8755..9282)
                     /locus_tag="IV329_000822"
     CDS             complement(8755..9282)
                     /locus_tag="IV329_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8940026.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9512..10318
                     /gene="proB"
                     /locus_tag="IV329_000823"
     CDS             9512..10318
                     /gene="proB"
                     /locus_tag="IV329_000823"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8940027.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10315..11562
                     /locus_tag="IV329_000824"
     CDS             10315..11562
                     /locus_tag="IV329_000824"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8940028.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11638..11982
                     /locus_tag="IV329_000825"
     CDS             11638..11982
                     /locus_tag="IV329_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940029.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12099..12425)
                     /locus_tag="IV329_000826"
     CDS             complement(12099..12425)
                     /locus_tag="IV329_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940030.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13040..14242)
                     /locus_tag="IV329_000827"
     CDS             complement(13040..14242)
                     /locus_tag="IV329_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8940031.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14359..14874
                     /locus_tag="IV329_000828"
     CDS             14359..14874
                     /locus_tag="IV329_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8940032.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15073..15519)
                     /locus_tag="IV329_000829"
     CDS             complement(15073..15519)
                     /locus_tag="IV329_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8940033.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15566..17044)
                     /gene="msr"
                     /locus_tag="IV329_000830"
     CDS             complement(15566..17044)
                     /gene="msr"
                     /locus_tag="IV329_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8940034.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17398..18027)
                     /locus_tag="IV329_000831"
     CDS             complement(17398..18027)
                     /locus_tag="IV329_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8940035.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18011..18475)
                     /locus_tag="IV329_000832"
     CDS             complement(18011..18475)
                     /locus_tag="IV329_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8940036.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18962..20230)
                     /locus_tag="IV329_000833"
     CDS             complement(18962..20230)
                     /locus_tag="IV329_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8940037.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20461..21198)
                     /locus_tag="IV329_000834"
     CDS             complement(20461..21198)
                     /locus_tag="IV329_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8940038.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21265..21834)
                     /locus_tag="IV329_000835"
     CDS             complement(21265..21834)
                     /locus_tag="IV329_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8940039.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
BASE COUNT         6089 a         4694 c         3973 g         7106 t
ORIGIN      
        1 atgctgaatt gctaaaggtt cttggaatgt gatctctgct aaaaagtctg gtccttctgt
       61 ctctattttt tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg
      121 cttcctcaac agagaataga tttgattgct tataacgctt ttataataac ttccaaagct
      181 aacacctttg cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg
      241 ttcccaatcc tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag
      301 tggaaaatat ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg
      361 caataactcc tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc
      421 atcaatgatt catcgaactg attagaacga tatgttattt attttttctc attaaatcgt
      481 ccaatttttg ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat
      541 caataaaatg catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct
      601 tatataattc ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg
      661 ctaaatcgct tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc
      721 tctcgatata actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt
      781 tcttataatt ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca
      841 ttccttcata tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca
      901 ttttgtcctg ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt
      961 ctacaatcaa cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat
     1021 acataagcaa accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga
     1081 tcgtcagcaa ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac
     1141 caatgactaa agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat
     1201 ctgtgccaaa tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg
     1261 tcacgcgtgc aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg
     1321 tgattccaac tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg
     1381 tccgtaaaat cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg
     1441 ggtcttcttt tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag
     1501 gtgtaatcgc aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt
     1561 gtttaggaac ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta
     1621 ctttttctcc tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg
     1681 cttctactgc ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat
     1741 cagaaactga ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt
     1801 cttcaaatcc acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca
     1861 actttctgaa tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc
     1921 agatagccca tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt
     1981 tttgcgctcg tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct
     2041 gtcaaaagat gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca
     2101 taattcgttg ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg
     2161 tcacgaacat agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat
     2221 tgtgtgtaat ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata
     2281 agatcccttt ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc
     2341 ttcttgcttg atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg
     2401 ctgtaaataa aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca
     2461 agacagcggc agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat
     2521 agaccactgt gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct
     2581 gttctaaatt ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag
     2641 tttcttggcg gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg
     2701 cttctttcat cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga
     2761 agaaacgcaa gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc
     2821 ctagagattt actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc
     2881 agtaattagc gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat
     2941 gaggaaattc taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg
     3001 ttgccataac ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc
     3061 aagaaatctc gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt
     3121 gctgctgttc ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt
     3181 cacttaattt cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt
     3241 cataagcata gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat
     3301 ccatgactct tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa
     3361 atgctcggat gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac
     3421 ggatgatttt atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat
     3481 attcaaaata gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga
     3541 tataattata cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga
     3601 tgaattcttc tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac
     3661 tttttctcct ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg
     3721 aacatctttc aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg
     3781 tcctaatatc tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt
     3841 acccgtttct tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc
     3901 gatttctgcg gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc
     3961 aatcgttgct cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat
     4021 cttcgccaat aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa
     4081 ggcatgaaaa ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt
     4141 tctctttgct gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa
     4201 gaattttatg taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc
     4261 cttatcaaaa gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg
     4321 acagctaacg cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg
     4381 ttttctgtgt ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct
     4441 tgcaccaccc ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt
     4501 ttagttcagt acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt
     4561 ttcaggcaat tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag
     4621 attttttcct tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg
     4681 agcatctact acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac
     4741 tgtttcacca gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa
     4801 tagcgtagac agatcaacga tttctgcagc atagctcatt tgaggttgat acaggctaac
     4861 gatcttcttc acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc
     4921 gattagataa ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg
     4981 gttgtttacc cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt
     5041 tggatcgaac attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga
     5101 ccagcccaat aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata
     5161 ttgttcgata aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact
     5221 gttgatgatc aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat
     5281 catcagttgt tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt
     5341 catcaaatga tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg
     5401 gatgacaaag tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc
     5461 tgtaaatgcg tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg
     5521 tgcttctttt tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg
     5581 catttctcct cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata
     5641 gcatttgtat gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt
     5701 acgttcagat tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt
     5761 cattcccaac caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt
     5821 ttggtcagta tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata
     5881 attgaataat gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata
     5941 gcgtacgcgt actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc
     6001 aaaaattccg tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat
     6061 tttacctttt ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg
     6121 aatttctttt ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc
     6181 ctcgtcctgc atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac
     6241 ttgttacaac gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca
     6301 tcgtgccgtc atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta
     6361 gaacattcat cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga
     6421 tattaagaac aggtacattt tggaattcag agactttatt caagttatag tcattggtta
     6481 caatgacacc atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat
     6541 cttcaaagtc tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta
     6601 ggatatctaa tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt
     6661 attgtagttc gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct
     6721 ttgtaatatc ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat
     6781 ggaaattttc tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt
     6841 gctcgatttt tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc
     6901 ccattctcaa tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga
     6961 cgaatggaat ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga
     7021 tgatcgcacc gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt
     7081 ctacttgttt gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa
     7141 taagtgcgcc taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac
     7201 cgattgcctg ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca
     7261 tcagcaaagt aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta
     7321 gttagcggaa cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc
     7381 cttttggcgc ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga
     7441 gtttttggac ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca
     7501 gtccaatttc accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag
     7561 caatactgac agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag
     7621 cttttaagta agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca
     7681 tgatcagaga tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca
     7741 tagtaggtgt gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca
     7801 caacgatagc agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat
     7861 tagcgacttc ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc
     7921 caaatcggtt ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat
     7981 ataacaccgt atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg
     8041 taacatgacc gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg
     8101 cagtggtttc tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca
     8161 tcgtttggat ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga
     8221 tttcgttcat atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt
     8281 ccgcgcgtaa ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc
     8341 ctatggctgc taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat
     8401 cgccaccaat caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt
     8461 cttctaattt tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg
     8521 gttttgcttt ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga
     8581 tttcttcgac catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg
     8641 acacataccc gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc
     8701 ctccttttat cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt
     8761 actggaagaa ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa
     8821 aaacggcaaa aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa
     8881 accaaagttg gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc
     8941 actgtcaatc acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga
     9001 acggttagca agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac
     9061 gagggttggt ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac
     9121 tgtttccaac ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc
     9181 tgcttcaaaa tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg
     9241 atctttatat ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat
     9301 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
     9361 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt
     9421 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
     9481 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
     9541 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
     9601 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
     9661 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
     9721 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
     9781 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
     9841 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
     9901 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
     9961 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
    10021 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
    10081 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
    10141 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
    10201 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
    10261 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
    10321 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
    10381 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
    10441 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
    10501 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
    10561 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
    10621 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa
    10681 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
    10741 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
    10801 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
    10861 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
    10921 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
    10981 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
    11041 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
    11101 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
    11161 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
    11221 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
    11281 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
    11341 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
    11401 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
    11461 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
    11521 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
    11581 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
    11641 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
    11701 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
    11761 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
    11821 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
    11881 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
    11941 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
    12001 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
    12061 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
    12121 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
    12181 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
    12241 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
    12301 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
    12361 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
    12421 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
    12481 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
    12541 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
    12601 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
    12661 caacctcttg gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta
    12721 aaatatgaga agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt
    12781 tacaacctct gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc
    12841 aaaatatgag gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt
    12901 tacaacctcc tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
    12961 cgaaaaatat gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt
    13021 tttcacaacc tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa
    13081 tgatacaaca tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac
    13141 caatatgttc accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga
    13201 caaaactata gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg
    13261 tcgtcaacag tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta
    13321 caaggactgg catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa
    13381 atgctgctaa aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa
    13441 tcgaagcaat agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag
    13501 aatagccaat caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata
    13561 acaatgcgat tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc
    13621 aaaggcctac ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa
    13681 aaccgcaaaa catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca
    13741 agaccccacc gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca
    13801 tgattgcttt ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga
    13861 caaataaagc attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc
    13921 cagataatcc gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt
    13981 ttttcaaacc gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta
    14041 acatgtaact agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa
    14101 tcgtttttaa gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg
    14161 ctacaactag cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta
    14221 tttgattctc caatatttcc atccctttct ctctattagc atcatacatg atttatttta
    14281 catgtcaata tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag
    14341 acgagaggta ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc
    14401 aaaccacgtt ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac
    14461 agtcagatct atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac
    14521 aaagacgagc actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta
    14581 gaggaatgga tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa
    14641 atctatgctt tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa
    14701 caacagctga acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac
    14761 aagaaacaag aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag
    14821 gaactccaat ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata
    14881 aacagaacct ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata
    14941 cagttggaaa agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat
    15001 ttttctagaa ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat
    15061 aacagtgttc catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc
    15121 aacgatgatc aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata
    15181 agcagagaat aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca
    15241 tgtcaccatt cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc
    15301 tggatttact ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc
    15361 caaaatgaaa gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag
    15421 atcttgatgc agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat
    15481 tgttgtaaaa attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt
    15541 tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct
    15601 gcccttttat ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag
    15661 taaacaaaat cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca
    15721 tatcaataaa attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca
    15781 aagcaatcgt cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag
    15841 tttcctgagc aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg
    15901 tttgtctcat gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct
    15961 gatatacttg aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat
    16021 gttctaataa ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta
    16081 aagaaaactg aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat
    16141 tttgtcccat gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga
    16201 tttgtttcat ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg
    16261 ttttcgcatt cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc
    16321 gatccagtct gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt
    16381 gttttttctt ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg
    16441 cttctcgtag ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc
    16501 ccgaaaactc tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta
    16561 gaaaatagcg atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa
    16621 tcagtcgatc aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt
    16681 gttcataaga agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt
    16741 tccgctctga agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt
    16801 cctttgcttc agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga
    16861 ttttcccatg atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga
    16921 caccattttc tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag
    16981 atagttctag ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt
    17041 ccatatgttt tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca
    17101 gaaaaatctg tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta
    17161 tgactaaatc accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg
    17221 acatactcca tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat
    17281 tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta
    17341 ggttaccaca cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta
    17401 ttcgaacata gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc
    17461 ttttcctatt gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc
    17521 tgccaactga tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc
    17581 aactaacatg aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc
    17641 ttctcttgaa aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc
    17701 ttttggcatc ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca
    17761 ttgcatatcg ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa
    17821 acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca
    17881 tggtttcata ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc
    17941 ttcgtgtggg attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat
    18001 tgttgaaaaa tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat
    18061 tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc
    18121 tgaaagccgg ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt
    18181 tcttctgggc tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct
    18241 gacgcgaaac atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat
    18301 caatctgcaa cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca
    18361 tcatgatctc gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt
    18421 tttgatcggc taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt
    18481 cctcctttct aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga
    18541 aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga
    18601 aaagaaattt tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca
    18661 agattattga ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac
    18721 cgccaaaaat ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc
    18781 agtatgctta aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt
    18841 aaacggtgtt caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa
    18901 atctgttttt gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt
    18961 tttaataacc ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc
    19021 tttccttagc actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc
    19081 gataaattgg cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt
    19141 ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat
    19201 tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt
    19261 cccagcctac agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc
    19321 ctgtatgacg gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact
    19381 gtgtcagatt ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg
    19441 cataacgacc tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg
    19501 caccggaata gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat
    19561 cttgcaatga ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt
    19621 gcccattcac atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa
    19681 tcccaaatag attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa
    19741 cgatggcttg agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg
    19801 cagttgaggc aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa
    19861 aagcatagcc agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg
    19921 tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt
    19981 ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg
    20041 ttgaaattgt attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa
    20101 ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta
    20161 gcactgttgt gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct
    20221 ttgctttcat cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta
    20281 aaaaagtaaa agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt
    20341 tgctttttct gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat
    20401 tgatggtcgt ttgaataact atttaacaag taattctctc tggatattat ccgttatgga
    20461 ttatacttcc tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt
    20521 ttctatcgca aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg
    20581 cgcttcatga agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg
    20641 aacagctcct ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa
    20701 tccttccatg attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata
    20761 aacaacatgt cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat
    20821 gattggaagt acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt
    20881 ttgccattca tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt
    20941 gtacaataca tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg
    21001 tgcaaaatct cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt
    21061 tattgctttt aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc
    21121 taatagcttc gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt
    21181 ttctgataaa tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca
    21241 ctatctctta tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc
    21301 agggattcct gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc
    21361 aacaatggca ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca
    21421 agcatttttc ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt
    21481 tagatggttc accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca
    21541 gatgccacct aagtcaacga acatcacttg acgattgata aagacatttt tccctatact
    21601 aatatgtttg ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt
    21661 gtcactaatg atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt
    21721 cagcgctgct aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg
    21781 atccgttact cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct
    21841 ccctcctttt atccttactt ta
//
LOCUS       DACDKL010000028        21317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDKL010000028 DACDKL010000000
VERSION     DACDKL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.028"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(6..596)
                     /locus_tag="IV329_000836"
     CDS             complement(6..596)
                     /locus_tag="IV329_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8940040.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            1080..1307
                     /locus_tag="IV329_000837"
     CDS             1080..1307
                     /locus_tag="IV329_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8940041.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1323..2489
                     /locus_tag="IV329_000838"
     CDS             1323..2489
                     /locus_tag="IV329_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8940042.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2723..3766
                     /gene="hrcA"
                     /locus_tag="IV329_000839"
     CDS             2723..3766
                     /gene="hrcA"
                     /locus_tag="IV329_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8940043.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3860..4423
                     /gene="grpE"
                     /locus_tag="IV329_000840"
     CDS             3860..4423
                     /gene="grpE"
                     /locus_tag="IV329_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8940044.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4474..6303
                     /gene="dnaK"
                     /locus_tag="IV329_000841"
     CDS             4474..6303
                     /gene="dnaK"
                     /locus_tag="IV329_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8940045.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6454..7620
                     /gene="dnaJ"
                     /locus_tag="IV329_000842"
     CDS             6454..7620
                     /gene="dnaJ"
                     /locus_tag="IV329_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8940046.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7702..8367)
                     /locus_tag="IV329_000843"
     CDS             complement(7702..8367)
                     /locus_tag="IV329_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8940047.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            8343..8516
                     /locus_tag="IV329_000844"
     CDS             8343..8516
                     /locus_tag="IV329_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8940048.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(8561..9298)
                     /locus_tag="IV329_000845"
     CDS             complement(8561..9298)
                     /locus_tag="IV329_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8940049.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9300..10802)
                     /locus_tag="IV329_000846"
     CDS             complement(9300..10802)
                     /locus_tag="IV329_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8940050.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11061..13055
                     /gene="uvrB"
                     /locus_tag="IV329_000847"
     CDS             11061..13055
                     /gene="uvrB"
                     /locus_tag="IV329_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8940051.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            13065..15881
                     /gene="uvrA"
                     /locus_tag="IV329_000848"
     CDS             13065..15881
                     /gene="uvrA"
                     /locus_tag="IV329_000848"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8940052.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16144..17409
                     /locus_tag="IV329_000849"
     CDS             16144..17409
                     /locus_tag="IV329_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8940053.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17413..18144
                     /locus_tag="IV329_000850"
     CDS             17413..18144
                     /locus_tag="IV329_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8940054.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            18279..19163
                     /gene="rapZ"
                     /locus_tag="IV329_000851"
     CDS             18279..19163
                     /gene="rapZ"
                     /locus_tag="IV329_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8940055.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19160..20158
                     /locus_tag="IV329_000852"
     CDS             19160..20158
                     /locus_tag="IV329_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8940056.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20192..21127
                     /gene="whiA"
                     /locus_tag="IV329_000853"
     CDS             20192..21127
                     /gene="whiA"
                     /locus_tag="IV329_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8940057.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
BASE COUNT         7018 a         3720 c         4603 g         5976 t
ORIGIN      
        1 aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc aagtttgtat
       61 agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg aagaatcaag
      121 tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc tttcacagct
      181 tgttgttcta taaaatcttc tttattctga taattttccg attctaacca aggaaattgc
      241 gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata atcaactgcc
      301 ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat ttgatataaa
      361 tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc aactttttca
      421 ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga agtgacctct
      481 gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt gctagtaata
      541 taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt tttcattttc
      601 aagctctcct ttactatcta taacaaggac aacagtttta acctacagac attcattaaa
      661 attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat acagatagtc
      721 aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt taccagcatt
      781 ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg agtacaatat
      841 taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca tatcgggcac
      901 tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat gaaagaaaaa
      961 atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa tacgtttagt
     1021 atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag gaggaatctg
     1081 tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc agctttgcga
     1141 ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat aagcaagcat
     1201 ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag ttggtgcttg
     1261 aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga gaaagaattt
     1321 caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt catatcccat
     1381 tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa gggcagccag
     1441 tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa agtcatccga
     1501 cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta tctgctaaac
     1561 aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat ggtaatgagt
     1621 ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta atgaaaaact
     1681 atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta ttgaaaaaaa
     1741 ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta gaagcagaaa
     1801 attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca ctagaaggat
     1861 atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct ttgtactcat
     1921 tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga ctcgaactgc
     1981 ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg gaaaagcatg
     2041 gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct caacataacc
     2101 tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc ggttatcttg
     2161 gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca ttacgagcaa
     2221 atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata gaagaagaaa
     2281 tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat gatcgctatc
     2341 aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa gaagggttac
     2401 ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg gggaacaatg
     2461 tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt atctgaattt
     2521 aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat aactggattt
     2581 acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg gagtctttct
     2641 cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta gaatatatga
     2701 gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt tacgtctgat
     2761 catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga tgtctgaggg
     2821 agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag atgaaggatt
     2881 gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct atcgttatta
     2941 cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc tgattcgtca
     3001 gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg ctgagatatt
     3061 atctgagctg acaagctata cgactttttc attgggacct gaaataaaag accgccgact
     3121 gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg tcgtaacaga
     3181 tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa gtcaggaact
     3241 agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga tgacggtgta
     3301 ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa cgacagaagg
     3361 gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg tttatgtcag
     3421 tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt tcaaatcgat
     3481 gttttctttt atgaaagact cagacggact tacacaaatg atcactccga tggatagcaa
     3541 tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata tgagtatgat
     3601 tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt tagggcctac
     3661 tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag aactagcagc
     3721 taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg aataacgaag
     3781 ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc tgactgaata
     3841 atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga
     3901 tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta tttcagaagc
     3961 tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa tggaagataa
     4021 atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga
     4081 gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat caattgacaa
     4141 cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg
     4201 cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat
     4261 ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc
     4321 gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct acaaattaca
     4381 tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg gatataaaac
     4441 ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg tattgactta
     4501 ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat catcgctaac
     4561 ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga aatccaagta
     4621 ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc aatcaaacgc
     4681 catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac accacaagaa
     4741 atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt aggtgaaaaa
     4801 gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg tcaagcaaca
     4861 aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga accaactgca
     4921 gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt agtattcgac
     4981 cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt ctttgatgta
     5041 ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa aatcatcgac
     5101 tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga caaaatggct
     5161 ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc
     5221 acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca tttggaaatg
     5281 acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg tacaaaagta
     5341 ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga cgaagttatt
     5401 ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa agaaacagga
     5461 aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc tgctatccaa
     5521 ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac accattgtca
     5581 ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa tacaacgatt
     5641 ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc agtagacatc
     5701 catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg aagattccaa
     5761 ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac attcgatatt
     5821 gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa
     5881 atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat ggtaaaagat
     5941 gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct acgtaatgat
     6001 gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg caaagtggac
     6061 gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc tgttgaagca
     6121 aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt tcaaaacttg
     6181 actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc agaagcagct
     6241 caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg cgacgataaa
     6301 taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt tttcttttga
     6361 aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata acaagcgacg
     6421 tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga ttattatgaa
     6481 gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta tcgtaagctt
     6541 tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa attcaaagaa
     6601 gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata tgaccaatac
     6661 gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg atttggtgga
     6721 ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga ctctttcttt
     6781 ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat
     6841 acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat caagtataat
     6901 cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac acaaccaact
     6961 acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac accacttgga
     7021 cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga aatcaaagaa
     7081 ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt taaagtgaat
     7141 gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg agaagctgga
     7201 gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga aagtgatatc
     7261 tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt acaagcagct
     7321 ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa gatccctgct
     7381 ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa gttacgtggt
     7441 ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa
     7501 caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt tgaagaacag
     7561 caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag
     7621 tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca atcatatgtt
     7681 cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaagccagct ttagaatatg
     7741 ataatcgtca tagtttctag gggtataacc agtgagagaa tggaacttgt tcaatcgata
     7801 ttgcaaggtg tttttatgaa taaataattc ttctgagcaa tttttgatgc ttccgttatt
     7861 tttactgaag catgccaaag tcttcttgaa caaatgtact tcttcttctg tcaggctttt
     7921 caaaatacgt tttacatata atgttcgttg atctgacggg attgctggta aaacgatccc
     7981 gaggtctaaa tcttcaaaaa agcggatctc gttcacagat tgatggaaag ctatccattc
     8041 gcaaacttgt ttggcatgaa ggaaagattg cggtgtctct tgctctgtat aacaaagcga
     8101 cccaattcca aagtttagct gtaactgata cattttatta aactttgtct ggattttatc
     8161 aagcaatgat agcaatgagg ctctggaaat ccgattgact aggataatga tttgttgtct
     8221 atgtacagtg agcaaagcct gtccgtaata agagaagacc ctttcgagca actccaataa
     8281 aggattgatc cgttcttgac acaattgctg attgtcagaa agttgtccca caactacata
     8341 atatggagaa gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat gtacttgata
     8401 tcatcaatga accgatggta ctttcttatg cgatggaaca ggtagagcga ttgcttgttt
     8461 gtgcaggaaa acaagcgatg cggctgatac gattaggtga gaaaataaga agataaaaat
     8521 acacggtccc ttcatggcga ccgtgcattt ttggttttta ttaaatattt aatactttat
     8581 tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa gatttgttga ggcgttccat
     8641 cttctaaaaa gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct ttagcgaacc
     8701 ccatttcgtg cgtaacgata accatcgtca taccttgttc tgctaacttt ttcatgacgt
     8761 tcaagacatc accgaccatt tcaggatcga gtgcagaagt aggctcatca aaaagcatga
     8821 tgtcagggtt catcgctaga gcacgagcaa tagccacacg ctgtttttgt ccaccagaaa
     8881 gcatttcagg atacatatct tttttatctt ctagaccaac tgtctgcagt aatttgattg
     8941 cttttttctc tgcttcttct ctagattctt tcttcaggtc catcggtgct aaaatgatgt
     9001 tttccaaaat agaaagatga gggaataggt taaagtgttg gaataccatt ccgatatgtt
     9061 gtctcaccaa gttgatatct gtgcttttat ccatcaagtg ggcgccgtca ataataattt
     9121 caccgtcaga aggttcttct aaacgattca agcaacgcaa gaatgtactt tttcctgaac
     9181 ctgaagggcc aatgatacag acgacatctc cttcgttgat ggatacattg atgtcgttta
     9241 atactgtgtt atcgccgtat tttttgacta aatgctcaac taagattttt tctgccatct
     9301 tacttcacct tcttttctaa aacttttgct aattttgtca atgctgtgat caaaatcaag
     9361 tacatgatcg caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc aacaatgatc
     9421 ttaccagttt gcagcaattc gactacaccg attgcggaaa tgatcgttgt gtcttttaat
     9481 gagataacga actgattgac gaatgatggg atcatgatgc gtatcgcttg cggcaaaatg
     9541 attttttgca ttgtccgatt ataagaaaga cctaaacttc tggaagcttc catttgtccg
     9601 acaggaacgg cgttgatccc gcctcgaaca atctctgcga tataagcact tgcgttaaga
     9661 gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa atccaacgat accaggcaac
     9721 ccaaagaaaa taaagaacgc aagaaccatc attggaattc ctcggatgat atcgacataa
     9781 atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc aaaaatcaca
     9841 ccgattacta tagccaaagc aaaggagatc aaagttagga gaattgttct ccaaagtcct
     9901 ttcaacaatg cttgatagtt gttttggata agtccaagaa atgttgattc atcagcagtg
     9961 gaagactcac tggaagtttc tgaaccatcc gctacatatt ctttaataat cttatcgtat
    10021 tcgcctgttc tttttaattc tttcaatcct tcattgaaca tattaagaag ctcaggattt
    10081 tgtccttttt taacggcaaa tgcatatttt ccgccagatt cttttttgat cggtgtttcc
    10141 aattcttgtc cttgtttgat cgcataaccg attaccggat aatcatccat cattgcatcg
    10201 attgcgccaa cttttaaccc gtcgtacaat tgatcagcag catcaaaata tttgatagaa
    10261 tagccatatt cagattcatg gtcgcttaaa aattcagcac tttcggtacc aacttttgct
    10321 ccgactgttt ttcctttcaa atcttcataa gacttgatcg aagtatctcc ttttttgaca
    10381 gctaattgga tcccgctatc aaagtaagaa tcagaaaaat catagctttt ctttcgttca
    10441 tctgttacag tcattccggc aatcattccg tctgcttgtc ctgattccaa agcttgcaaa
    10501 gcagcagaga aaccacggta gtccattcgg atgttaaatc cttgcagttt ggcgatgttt
    10561 tccatcaatt ttacatcgat gccttcgtac tcgccctttg cgttttgaaa ttcaaatgga
    10621 gcaaaagcag agtcactggc aattacgtat acgtcttttt tgggctctat tttttgcatt
    10681 gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac atgaccaaac
    10741 gcaaatgtca caagtcctgt cattaagaaa acaaatgagt gaaataaatg ctttcttttc
    10801 ataatcaacc tcctaaattt ccaatatttt attattatag gcgaaaagtg ctgaatcaga
    10861 caagactgta agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt atgttataaa
    10921 acataacatg tataataaga taaaaaagtg gcgaaagtat gttcggtgtg gtataatttt
    10981 ttagtaagga atgcctttgc tttccttttc tttttattgg tcgtaaaatg aaaaggaatg
    11041 aatgaaaagg aggacgaaga atgattgaga gagatacctc ccgacacttt gagcttgtct
    11101 cgaaatacca accggctggt gatcagcctg aagcgattaa ccaattagtg gatggcgtag
    11161 tcggcgggaa aaaagcacaa atcttattag gtgcgactgg aacagggaaa acatatacga
    11221 tttccaatct tattgagaaa gtgaataaac caacactgat tattgcccat aacaaaacat
    11281 tggctggtca gctgtacggg gaattcaagg aatttttccc taacaatgct gttgaatatt
    11341 ttgtttctta ttatgattat tatcagcctg aagcttatgt gccttcaagc gatacgtata
    11401 tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt acgacattcg gctactagtt
    11461 cattattaga aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt tttggattag
    11521 gctcgccttt tgagtaccaa aaacaagtag tttcgattcg acagggagca gaactagatc
    11581 gcaatcagtt gattcgtgac ttagtcagta tccaattcga aagaaatgat atagattttc
    11641 agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat ttttccggct tcaagagatg
    11701 aacgtgcctt acgtgtggag ttttttggag atgaggtcga acgtatccga gaagtcaatg
    11761 cactgacagg tgaagtttta ggagaaacgg aacacgtagc aatcttcccg gcgactcact
    11821 ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa tatcaaagcg gaattagaac
    11881 agcgtttaac tgtcttgcga aatgaaaaca aacttctgga agctcaacgt ttagaacagc
    11941 gaaccaatta cgatattgaa atgatgttag aaatgggcta cacgtcggga attgaaaatt
    12001 attctcggca tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta ttggatttct
    12061 ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt aaccatgcct caaatcagag
    12121 gaatgtataa tggtgaccga gcgagaaaac agatgctagt agattacggt ttccgtttgc
    12181 catctgcttt agacaatcgg ccacttcggt tagaagagtt tgaaaaacat gtcaatcaga
    12241 tcatttatgt ctctgcgacg ccaggaccat atgaacatga acaaacagat accgttatcc
    12301 aacaaatcat ccgtccgaca gggctgcttg acccagtgat cgaagttcgt ccaatcatgg
    12361 gacaaatcga tgatctagta ggcgaaataa atgaacgagt cgaaaaagac cagcgggtgt
    12421 ttgtaacgac attgacgaaa aaaatggcag aagacctcac agattatttc aaagaattag
    12481 ggatcaaagt caaatatttg catagtgata tcaaaacgct ggaacgaaca gaaatcattc
    12541 gagatcttcg attaggtgaa tttgatgtgt tagttggaat caatttgctt cgtgaaggat
    12601 tagacgtacc tgaagtttcc cttgttgcta tcttggatgc ggataaagaa ggctttttac
    12661 gtagtgaacg ttccttagtc caaacgattg gacgagctgc tcggaatgaa gaaggtaaag
    12721 taatcatgta cgcagacaaa gtaacagatt ctatgcgtct agcgatggat gaaacgtcaa
    12781 gacgtcgtac gattcagcag aagtacaatg aagaacatgg gatcgttcca aaaacgatca
    12841 ttaaagaaat ccgagatttg atttctatta cgaaagaatc ggaagatgat acaaaagaag
    12901 cggtacaagt ctcgtacgaa gaaatgacga aggaagaaaa agacacgtta ttgatgaaat
    12961 tagaaaaaga aatgaaagat gcggcaaaag ctttggattt tgaaacagcc gcgaatgtgc
    13021 gagatatgat tttagaatta aaagcttcaa agtaggagga caacatggct aacgataaaa
    13081 ttacgattca tggtgcacgt gcccataact tgaaaaatat agatgtaacg attccacgag
    13141 ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa aagttcccta gcatttgata
    13201 cgctgtacgc tgaagggcag cggagatacg tagagagcct ttctgcttat gctcgtcagt
    13261 ttttaggaca aatggataag ccggacgtag atagcatcga cggattgagt ccggcgattt
    13321 ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact gtgacggaaa
    13381 tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt cccaacgatc
    13441 atatcgagat taccagtcaa tcagtagatc aaatggcaga tcaagtaatg gctttgcctg
    13501 agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga caacataaaa
    13561 aaatattcga acgaatccaa aaagagggat acgtccgcgt tcgagtcgat ggtgagatct
    13621 atgatttaag tgaagtgcct gagctggaga aaaacaaaaa acatgatatc gcgatcgtca
    13681 tcgaccggat cattgtgaaa gaagggattc gttctcgcct atttgattct ttagaggcgg
    13741 ccctgcgatt agcagacggc tatgctctgg ttgacgtgat cggtgaagaa gaaatgttgt
    13801 ttagcgaaca ctatgcctgt ccttattgcg gatttactgt tggcgaacta gaaccaagac
    13861 tgttctcatt caatgcgcct tttggagctt gtccagactg tgatggttta ggtgtcaaat
    13921 tagaagtcga tcaagattta gtgattccag atagaacgaa gacacttagt caaggtgcta
    13981 tcgttccatg gaatccaatc agctctcagt attatcctca aatgttagcg caggcttgtg
    14041 aaagtttcgg tatcgattta gatacaccat tcgaagaatt accggaagag catcaggaga
    14101 tcatcttgaa tgggacagat gatcttttcc atttccatta tgaaaatgat tttggtggcg
    14161 ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa tatcgagcgt cgttatcatg
    14221 atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa ttaacttgtc
    14281 agacatgtaa aggttatcga ttgaatcccc aagctctatc tgttcaaatc aatggaacga
    14341 atatcgggca aacaaatgag ttgtctattc aaaaagcagt agatttcttc tcaaacttaa
    14401 cgttatctga acaagagaaa gtaattgcca aaccgatttt aaaagaagta aatgacagac
    14461 tttcattctt ggaaaatgtg ggactagact atctaacttt gagtcgcgct tccggaacat
    14521 tatctggggg agaagcgcag cgaatcagat tggctacaca aattggatcg aatctttctg
    14581 gagttttgta tattttagat gaaccatcga tcggattgca tcaacgcgac aacgatcgac
    14641 tgattggctc attaaagaaa atgcgtgatc ttggcaacac cctcgttgta gtcgaacatg
    14701 atgaagatac gatgtgggca gcagattatt tgattgacgt cggacctggg gccggagaac
    14761 aaggcggaca aatcgtggct gcaggcactc ctgaagaagt agctaatgat gaaaattctt
    14821 tgacagggaa atatctttca gggaaaaaat caatccctgt accaaaagaa cgccgtaaag
    14881 gtaatgggaa agcaatcaag atcactggtg cttcagaaaa caatctgaaa aatattagtg
    14941 ttgaatttcc attaggagaa tttgtagcag tcacaggtgt gtctggttct ggaaagagta
    15001 cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa attaaataag aattctgcta
    15061 agccaggtaa attcaagaca atttccggct acgaaagtat cgaaaagatc atcgatatcg
    15121 atcaaagccc aatcggccgg acgccgagaa gtaatccagc gacttataca agtgtatttg
    15181 atgatatccg tgggttattt gctcaaacga acgaggcaaa aatgcggggt tataagaaag
    15241 ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg gatggaatta
    15301 ttaagatcga aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta tgtcatggta
    15361 aacgatataa ttctgaaaca ttagaagtgc attacaaagg aaaaagcatt gctgatattt
    15421 tggaaatgac agtagaggat gctgtagaat tcttcaagca cattccaaag attcatcgca
    15481 aactgcaaac gattgttgat gttggcttag gttatgtgac gatggggcaa ccagcaacga
    15541 cattgtccgg tggtgaggca caacggatga aacttgccag tgaattgcac aaaatctcta
    15601 atggaaagaa tttctatata ctagatgaac caacgacagg acttcatagc gatgacatcg
    15661 cccgcttgtt gcatgtatta caaagattag tagatgctgg taacacagtt ttagtgattg
    15721 aacacaatct agatgtaatc aaaacagcag attatatcat tgatttagga ccagaaggtg
    15781 gagaaggtgg aggaacgatc cttgcgactg gaacaccaga agaaatcatt aacgtaaaag
    15841 aaagttatac aggtcactat ttgaaaaaaa taatggtata aagaggatag atagctgaga
    15901 tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt attaatatca gtgttctaga
    15961 atcattacat ggtagttctt tctacactga ttggtataga taaaaacttc ctataagttc
    16021 aatccgttca aaaaagaatt gagcaatcgg aggactttcc tttgattgct tttttcttta
    16081 tggtatgata ttccttgtgt agttggaaaa gaaaaacaag agattatgag taagtgagag
    16141 ataatgatga acagtattga aaatgaagaa tttatcccta tattattagg aagcgacatg
    16201 aacgtttatg gtatggcacg ttcttttaat gaagcatacg gcaaaatctg ccaagcatat
    16261 gcttcggatc agttagcccc tacccgctac agtaaaattg tcaatgtaga agttattcca
    16321 ggatttgata aagaccctgt atttatcgag acgatgcttc gtttggcaaa agaaagatat
    16381 tctgataaga gtaaaaaata tcttttgatt gcttgtggtg atggatatgc agaattgatc
    16441 tcccaacata aacaagagct gtcagaatat tttatctgtc catatattga ttattcttta
    16501 tttgaaaggc tgatcaataa agtaagcttt tatgaagttt gtgaagaata tgatcttccg
    16561 tatcctaaaa cattgatcgt cagagaagaa atgttagtaa atggacattt ggaacaggaa
    16621 ttgccttttg aattcccagt tgctttgaaa cctgcaaaca gtgtggagta tctttctgtc
    16681 caatttgaag gaagaaaaaa agcttttatt ttagagacta gagaagagtt cgatttaatt
    16741 ttaggccgga tatacgaagc cggttataaa agtgaaatga ttgtacaaga ttttatcccg
    16801 ggtgatgata gtaatatgcg tgtgctaaat gcgtatgtag atgaagatca tcaagttcgg
    16861 atgatgtgcc ttggtcatcc actattagaa gatcctacac cagcatcgat tggtaattac
    16921 gttgtgatca tgccagatta taacgaaaaa atctatcaaa ccattaaggc atttttggaa
    16981 aaaatcgaat atacaggctt tgccaatttc gatatgaagt atgatcctag agatggagaa
    17041 tataaattat ttgaaatcaa tttgagacaa ggtcgtagca gtttctttgt gacattgaac
    17101 ggcttgaatc tggctcgttt tgtcacagaa gatcgagtgt tcaacaaacc ctttgttgaa
    17161 acgacctatg gaacgaatca atcagataaa gcaagattat ggatgggtgt gcccaaaaaa
    17221 atctttttag aatatgctcg ggaaaatgaa gacaaaaaac tagctgagca aatgatcaaa
    17281 gaaaatcgtt acggtactac tgtattttat gaaaaagacc gatcaatcaa gcgctggttg
    17341 ttgatgaaat atatgttcca taactatatt ccaagattca aaaagtattt tcatgtgaaa
    17401 gaaggctagt tcatggagaa ttttttcagt attttaggcg gaatgggcac gatggcgaca
    17461 gaaagttttg ttcgcttgat caaccaccga acgaaagcta caaaagatca agaatatttg
    17521 aattatgtct tattcaatca tgcaacagtt cctgatcgta cagcttatat tttagatcga
    17581 tcagaagaaa atccaatgcc gtttctgctg gatgatattg agaaacaaaa tttattgcga
    17641 ccaaacttca ttgtgttgac ttgtaacacc gcacactact tttttgaaga gttacaagcg
    17701 gcaacggata ttcctatctt gcatatgcca agagaagcag caaatgaact tgtccgtcag
    17761 catacgacag ggagagtagc gattctagga acagaaggaa gtatgaaagc tggaatctat
    17821 gaacgcgaag tcaaaaatct tggtttcgaa acagtgattc ctgatactgc gcttcaagaa
    17881 aaaatcaatt acctgatcta tcatgagatc aaggaatcgg attatttgaa tcaagagctc
    17941 tattacgaaa tcttagagga agcggtagag cgcctaaact gtgaaaaagt catcctaggt
    18001 tgcacagaat tatctttgat gcatgaattt gctgaagata atcattaccc agtgatcgat
    18061 gctcaatcta ttttagcgga tcgaacgatc gaacgtgcat tagctgaaag aagcgaggca
    18121 ttggatacag catcggaaaa gtaaaaaaat tggatttggg gcaaatatcc taaaagatat
    18181 ttggtccaaa tctttttgta aaagaaaaag aaaactggta atgatacgcc cgggatgata
    18241 taattttatt atgcacaatg tgagaggggt tcgtaaatat gacagataat ttgcaattag
    18301 tcatcataac aggtatgagt ggtgcaggga aaacagtagc tatccaaagt tttgaagata
    18361 tgggctattt ttgtatcgat aatatgccgc ctcgattgat tccaaaattc tgggaattga
    18421 tcaaagaatc aggtaaagtc acaaaaatgg ctttaggagt ggatttgcgt tctcgttcct
    18481 tctttgaaga aatccaaaac atgctgatcg aaatcgaaaa tacgaacttc atcgacactt
    18541 ctatcttatt tttagatgct tcagacgaag aactagtggc tcgctacaaa gaaacaagac
    18601 gagcacatcc aatggcgatg gacggtttga tcactgaagg gatacgtaaa gaacgggcga
    18661 tactagacga cttgaaagcc caagcttccg tgatcattga tacgacaaca ttgacaccac
    18721 gacaattacg tgaaaaaatc aatgagtcat tcaaatcaaa gggagaccaa ggatttcgga
    18781 tcgaatttgt atcatttggt tttaaatacg ggttgccgat cgattcggat attgtgatgg
    18841 atgtgcgttt tttgcccaac cctcattata tcccggaact aagaccattg accggaaaag
    18901 atcagccagt ctatgattat gtgatgtcct ttgatgaaac gcaatcattc taccataaat
    18961 ttttggatct tctggaaact gtattgcctg gatacattaa agaaggaaaa agcagcttga
    19021 cagttgcaat cggctgtaca ggcggtcaac accgttctgt ggcattgact gaacgtttgg
    19081 caaatgcttt agggaaaaac tataaagtca atgtgacaca tcgggacaag gataaaagga
    19141 aagagacggt gaaccggtcg tgaaaatgaa aacctaccgg ataagaaaac caaagatcgt
    19201 agtggtcggc ggtggcactg gtctgccagt tatcctgaaa agtttacgaa atcaaggagc
    19261 agatatcaca gcagtagtga ctgttgcaga tgatggtgga agcagcggtg caatcagaga
    19321 atcgatcgct atggctcctc ctggtgactt gagaaatgtc ttagtggcgt tatcggatat
    19381 gccacaattc tatgaagata ttttccaata ccgtttcaaa aaggaagatc agtttttagc
    19441 caatcatacg atcggtaatc tgatcattgc cgcagtttcc gagatgcggg gaagcactta
    19501 tgaggcgatt caattattag caaaaatgat gcatgtagat ggacatgttt atccttcttc
    19561 agaaactcct ttgaccttgc atgctgtttt taaagacgga acgactgcag taggagaatc
    19621 taaaatcgcg atcaaccgta aaaccattga ccgtgtgtat gtctgcaaca caaatgataa
    19681 aagtgaagcc aaagcggctc gaaaagtcgt ttcagcaatt atggacgccg acatggtcgt
    19741 tttgggaccc ggaagtttat tcaccagtat ccttccaaat ttagtgatac cagaaatcgg
    19801 cgaagcaatg ctcaagacaa ctgctgaaac cgtctatatc tgtaatatca tgacccaaaa
    19861 aggggaaacg gaacatttca cagatgccga tcatatacgt gttttacaca agcatttgaa
    19921 tcagcctttt atcgatacag tattggtcaa tacggaaaaa gtgcctgaag attatatgga
    19981 cccagagatc tatgatgagt atctagtaca agtcgcacac gacttcaacg gattgagaaa
    20041 tgaaggatgt cgagtgattt ccaccgactt tttagaattg aaagatggtg gtgtcttcca
    20101 cgatggagac aaagtagtag aagaattgtt ccgtttagtt ttcggttcaa aatattaagc
    20161 aatttattgt tggagaaagg aggagaatag gatgtcattt gctgcagatg tgaagaaaga
    20221 actaactggt ttggaagttc atcgagaaca tgcgaaagcg gaattagctg ctttgctgcg
    20281 aatgaacggc tctttaggta ttgtgaacca gcagtttatt ttgaacgtcc aaacagaaaa
    20341 tgctgcaatt gccagacgta tctattctct tttgaaagat cattatcagg tacgaagtga
    20401 gctactcgtt cgtaaaaaaa tgaaattaaa aaagaacaat gtctatatcg tccggttgaa
    20461 gcaaggcaca aaagatgtgt tgatggatct tgatatcatg gatggtatga tgttcaatgg
    20521 acacgtttcg gacgagatta tgggaaattc gcaaaaaatg cgttcttact tacgtggtgc
    20581 ttttatggcc tcaggttcag tcaataatcc ggaaacgagt cgttatcatt tggaaatatt
    20641 ttctatttat gaacagcata accaagacat ttgcgatatg ttaaattatt acggtctaca
    20701 tgctcgaacg cttgaacggc gcaatggcta tatttcgtat ttaaaaggag cagaaaaaat
    20761 tgctgatttc ttaacattga ttggtgcaac caactcaatg ctccgatttg aagatgttcg
    20821 aatcgtacgg gatatgagaa attcggtcaa tcgtctagtc aattgtgaga cagcaaatct
    20881 gaacaagacg attgatgcgg cttctaaaca aatcgaaaac atcgaattga tcgaagcaaa
    20941 ggtcggtcta catgcccttc ctgaaaaact tcaggaaatc gcagaacttc ggcttcaaca
    21001 tccagaggtc agcttgaaag aattagggga aatgattccc tcaggtgcaa tatcaaaatc
    21061 gggaattaat caccgaattc gcaaaataaa tgaatttgcg gaaaaactgc gtgaagaagc
    21121 agtttaatcc aaagaaaaaa ctgatacatg aagcaggaga tgcgaatgta tcagtttttt
    21181 ttaaaagcta tacttgcttt tcttccgtca aactataatt ccaactttac tggagtaaca
    21241 taatgttgaa aggcttctat tgttttttaa tacaatcgta taaaaagcgg ggagacaact
    21301 gaatgaagat tttagtt
//
LOCUS       DACDKL010000029        21287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDKL010000029 DACDKL010000000
VERSION     DACDKL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.029"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..621
                     /locus_tag="IV329_000854"
     CDS             <1..621
                     /locus_tag="IV329_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8940058.1"
                     /translation="QEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLN
                     KKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILL
                     GDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDS
                     GQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1195..1764
                     /locus_tag="IV329_000855"
     CDS             1195..1764
                     /locus_tag="IV329_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8940059.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1751..2611
                     /locus_tag="IV329_000856"
     CDS             1751..2611
                     /locus_tag="IV329_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8940060.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2801..3505
                     /locus_tag="IV329_000857"
     CDS             2801..3505
                     /locus_tag="IV329_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940061.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3510..5345
                     /gene="walK"
                     /locus_tag="IV329_000858"
     CDS             3510..5345
                     /gene="walK"
                     /locus_tag="IV329_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8940062.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5342..6658
                     /locus_tag="IV329_000859"
     CDS             5342..6658
                     /locus_tag="IV329_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940063.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6659..7528
                     /locus_tag="IV329_000860"
     CDS             6659..7528
                     /locus_tag="IV329_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940064.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7583..8392
                     /locus_tag="IV329_000861"
     CDS             7583..8392
                     /locus_tag="IV329_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8940065.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8495..9124)
                     /locus_tag="IV329_000862"
     CDS             complement(8495..9124)
                     /locus_tag="IV329_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8940066.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9294..10586
                     /locus_tag="IV329_000863"
     CDS             9294..10586
                     /locus_tag="IV329_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8940067.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10662..11324)
                     /locus_tag="IV329_000864"
     CDS             complement(10662..11324)
                     /locus_tag="IV329_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8940068.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11549..11833
                     /locus_tag="IV329_000865"
     CDS             11549..11833
                     /locus_tag="IV329_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8940069.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11884..13509
                     /gene="groL"
                     /locus_tag="IV329_000866"
     CDS             11884..13509
                     /gene="groL"
                     /locus_tag="IV329_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8940070.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13598..14746)
                     /locus_tag="IV329_000867"
     CDS             complement(13598..14746)
                     /locus_tag="IV329_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8940071.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14848..15432)
                     /locus_tag="IV329_000868"
     CDS             complement(14848..15432)
                     /locus_tag="IV329_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8940072.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15559..15990)
                     /locus_tag="IV329_000869"
     CDS             complement(15559..15990)
                     /locus_tag="IV329_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8940073.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16008..16328)
                     /locus_tag="IV329_000870"
     CDS             complement(16008..16328)
                     /locus_tag="IV329_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8940074.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17066..17323
                     /locus_tag="IV329_000871"
     CDS             17066..17323
                     /locus_tag="IV329_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8940075.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17294..17602)
                     /locus_tag="IV329_000872"
     CDS             complement(17294..17602)
                     /locus_tag="IV329_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940076.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17680..18426
                     /locus_tag="IV329_000873"
     CDS             17680..18426
                     /locus_tag="IV329_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8940077.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18441..18644
                     /locus_tag="IV329_000874"
     CDS             18441..18644
                     /locus_tag="IV329_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8940078.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            18749..19048
                     /locus_tag="IV329_000875"
     CDS             18749..19048
                     /locus_tag="IV329_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940079.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19048..19230
                     /locus_tag="IV329_000876"
     CDS             19048..19230
                     /locus_tag="IV329_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940080.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19236..19712)
                     /locus_tag="IV329_000877"
     CDS             complement(19236..19712)
                     /locus_tag="IV329_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940081.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            19765..20490
                     /locus_tag="IV329_000878"
     CDS             19765..20490
                     /locus_tag="IV329_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8940082.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            20664..21005
                     /locus_tag="IV329_000879"
     CDS             20664..21005
                     /locus_tag="IV329_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940083.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            20998..>21287
                     /locus_tag="IV329_000880"
     CDS             20998..>21287
                     /locus_tag="IV329_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8940084.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7190 a         3571 c         4285 g         6241 t
ORIGIN      
        1 caagagatct tgaaattact tgacattttg tcagaagaaa aactctattt ggtagttctt
       61 ttaacagcat tttatggatt gcgaagaagt gaagttttag gattaaaatg gtctgctatt
      121 gattttttaa acaaaaaaat catgataaac catgtaatca tagaaaatcc agagaatatg
      181 tcacagttaa taaagaaaga tcaaacaaag aactcttcca gctatcgtac attacctttg
      241 gttgaaacga ttgaacgagc attagtaaat caagctaaat ggcaacaaga taatcgtatt
      301 cttttaggag atgattatat tttagaagat aatgagtacg tatttactat ggaagatgga
      361 cggttgatga agcctcaata tgtaacgcat cgtttgagta agttgattaa gaaaaatgga
      421 ttgaaaaaaa ttcgctttca tgacttgcgt cattctaatg cttcaataat gttagatagt
      481 ggccagaata tgaaatccat tcaagaatgg ctgggacatg catcgtattc gacaactgct
      541 aatttataca cgcatttaac cgcaggagta aaagatcgtg cagcagaagc attagaaaac
      601 gcatttgggt ttaaagaata aaaaaacaaa aaacaatatc tttggggtat cgcttcttgt
      661 ttttagaatt tttttgttgg caatttgttg gcagtatgtt ggcaatgaaa aaattcacaa
      721 actaaaaacc tatttataaa gctttataaa acttttttca caaatagagc atttggttaa
      781 gagtcagctg ctctaccaac tgagctaacg gcccgtacaa aaagtactta ataatagtag
      841 cactaagaat tttaaaatgc aagactacat ttaaagaaaa tcagaaaaat ctttacttcc
      901 ttataggaaa tcattcatag attattcaca tttgctcgct attatataag cataccaaca
      961 aacaaagttc ttgcaagaga acaaacccta acactttttt cattttttac tcctttcccg
     1021 ccgtctcccc ggcgggtctc ctgttcttaa cgataaaaga caagaaggct tagccttctt
     1081 gttttttgtt ttcatggaat cccgctaatc aaatcaccta gttaaagcaa ctagattgaa
     1141 tgtttatcat aagtctgata tcatgtttat gaaatgaaaa aaggagcggt tatgatggaa
     1201 aaaatgcgtc agcaattaat ggagtggggg aaggaactag aaacattccg tctgcctcac
     1261 tgggaagaac tccctgactt agaactttat atggaccaag tcagaacgtt agttgatcgt
     1321 tatctttcac ctgtgataca aggagaaaag catccattgc ttacctcttc tatggtaaac
     1381 aattacgtta aactaggttt gataccagca ccagttaaaa aaagatataa caaagaacac
     1441 gtagcatttt tacttgcaat cactacgttg aaacaagtat tgactattcc tgaaatcaaa
     1501 gaagggatat tgttccaagg aaaaacagta ggaattcgtg aagcctataa tctattttgc
     1561 gatgaacaag aagcagctgt gtggatggtc agccaattag cacaaggtaa atctcatccg
     1621 caaaaattcg accaggcaac ccctgtagaa tatattgctg taagagcggc aactttatcg
     1681 tttgcaatga aacttcttgc ggaaaaaacg attgttctag aaaccgaata tttaaaagag
     1741 gagaaaacaa atgaaaaaca ataaaatcgc aattttagta gattcaggta cggatgttcc
     1801 agaagaattg ataaaaaaat acgagatgta tatgatccca ttgaaaatta tttataaaga
     1861 tcaggtatat acagataaaa ttgacattac ccctgaagag atctatcaac gtttgccaga
     1921 ggaaatccct ggtacatctt tgccggatgg tgagtcgatc acagaaatct ttgagcaaat
     1981 caaaaaagac ggttatgaaa aggtacttgc ggtaacaatt tccagtggat tgagcgggac
     2041 atataatgtg gttcgtttaa ttgcacaaca gcaagaagaa ttggaagcat atgtactaga
     2101 tacgaaaaat attggtattg gtgcaggttt ttctgcgatc caagcagcaa aatggctgga
     2161 agaaggagta gaatgggatc aactgatcag taatctgaat gagttagtcg aaagaacaaa
     2221 agttttcttt aatgttgcga cattggaata tcttcaaaaa ggtggacgga tcggtttagt
     2281 tgcttctatt gtaggaaccg ctttaaagct taacccaatc atctcatgta atgaagaagg
     2341 gatttattac acagtaggaa aagctagggg acgtaaaaaa agtttggata aaacggttgc
     2401 ttacgtcaaa gaaaaaatcg gtaatgcaaa ggtcttcaac ttagctgttg cccatgggga
     2461 tgcgaaagaa gaagcaattg aaatggaagc gagacttaaa agagagttcc cacaagcaaa
     2521 ccaaatctac tttggccaga tttctccggc attagtagtc cacactggtc cgggattatt
     2581 gggtgtcggt gtacaagttt tagaaggata aatcaaaaaa aggaaacact gaggtgaaaa
     2641 tcagtgtttt ccttttttat tgaagttaga acaaaaaata ataaagtatt ttgcactttc
     2701 attttaattt tctcttttgt tttataggca aagatgaaac aaaagcagaa atacggtata
     2761 atgagaaggt atgacaataa tatataagag gtggctcttt atgaaaaaag ttttagttgt
     2821 cgatgatgag aagccgattt cggatatcgt caaattcaat ttagccaaag aaggctatga
     2881 cgtttatact gcatatgatg gggaagaagc cttggaaaaa gttgctgagg tagaacctga
     2941 tttgatttta cttgatttga tgctacctaa aatggacggc ttagaagttg ctcgtgaggt
     3001 acgcaaaact tatgatatgc cgatcattat ggtaacagcc aaagattctg aaattgataa
     3061 agtcctaggc ttagagttag gcgccgatga ttacgtgacc aagccatttt ccaaccgaga
     3121 attagttgca cgtgtcaaag caaatcttcg tcgcggtgcc acggctgcga aggaaccaga
     3181 agaggcagca cctgcggaat tgacgattgg tgatttgacg atccatccag aagcttacat
     3241 ggtgacgaaa cgaggagaaa caatcgagtt aacacatcgt gaattcgaac tactctttta
     3301 cttggcgaag caccttggac aagtgatgac aagagaacat ttattacaaa cggtttgggg
     3361 ttatgattat tttggagatg tacgaacagt ggatgtaact gtacgtcgtt tgcgtgaaaa
     3421 gatcgaagat aatccaagcc atccaaatta tttagtgaca cgtcgtggtg tcggttacta
     3481 tttgcgtaac ccagaacagg agtaattgga tgaaaagaaa agtccgcttt tttcgatcag
     3541 tcaattttaa gatcgctatt acctttattc tgattttatt gatctcgatc gaaattattg
     3601 gtgcctactt tatacgtggt ttagaacgtt ccacgatcaa tacatttatt aaagatatga
     3661 atcaaacggt ggaatcatta gcaacaacaa tcagcccgga attgaatcga aaagataatg
     3721 cagatgatga agaggtaaat gcgaatatca aaagatttat tgaaaacagt gcgactagcg
     3781 atatcatcga aatacgggtc gttgacgaaa aagggatcat ccgaggaaca accgatgtca
     3841 atgaacaaag cgctgtggga aagaaaaatg attatgttga tatcaatgat ttcacgacta
     3901 aacgttatgt tgctttagat aacgacgata aacgggtgaa tatcaatgtt caaccgatcc
     3961 tgtccccgac cggcgatgcc gttatcgggg ctctttacgt caaaagtaat atcgaacaaa
     4021 aatattcgga aatcaataac acagcagtca ttttctttac tgcctcacta attgcggcat
     4081 ttatttcaat gatcgtatct gttttagttg ctcgttcgat tacacagccg atcggtgaaa
     4141 tgagagaaca agccattcga atcgctagag gagactacag tcggaaagtc aaagtgcatg
     4201 gacaagatga acttggacag ctggctgata ccttcaatca gttagctgaa cgtattgaag
     4261 aagctcaaga tacaatggaa gctgaacgca atcgtttgga tagcgtgcta tcccatatga
     4321 cagacggcgt cattgcgaca gatcgcaggg gtaaagtcat tacgatcaat gatatggctg
     4381 tttccttgct agatatcaaa aacgaggaag caatcggaca atcgattttg acacttttag
     4441 atatcgaaga agaatatacg ttgcggaaat tattagaaga tccaaacgaa atggtacttg
     4501 accgttcaac cagtacattg gaaagtgatc agatgatctt gcgagtagac ttttctatga
     4561 tccgtcgcga atcaggtttt atcagcggat tagtagctgt cttgcatgat gtgacagaac
     4621 aagaaaaaac agaacgggaa cgtcgtgaat tcgtctctaa tgtttcccat gaacttcgta
     4681 caccgttgac aagtatgcgt agttatatcg aagcattgag cgaaggtgcg tggaaagacg
     4741 aggaagtagc accaaatttt ttgaaggtaa cgctagatga aacagaccga atgattcgaa
     4801 tgatcaatga cctcttaaat ctatctcgca tggatacagg taatacccag cttcaattag
     4861 aatatgtcaa tttcaacgag ttagtgaatt ttgttcttga ccggtttgat atgatggtgg
     4921 gaaatcagga aaaaaattat aagatccgtc gtgaatttac gcaaagagat ctttgggtcg
     4981 aagtagatac agataaaatc atccaagtag tcgataatat catgaataac gcgatcaagt
     5041 attcaccaga tgggggaacg attacttgtc gcttattaga aacgcataat aacgtgattt
     5101 taagtatcac cgatcaagga ttggggattc ctaaaaaaga tctgaaccga gtgttcgagc
     5161 ggttctatcg agtagataaa gcacgtgcaa gagcacaagg tgggacagga cttggattag
     5221 ctatttctag agaagtgatt aaggcgcatc gaggcgctat ttgggcagag agcaaggaag
     5281 gaaaagggtc gactttttat atctcgctgc catatgaacc ttatgaggag gaatggtggg
     5341 aatgagaatc tctgaaaaag tcgttcgtat aggactcatt ttgatggttg cgttaagcat
     5401 atacttctct tatgcaatct ggctgagccc agcaggaaaa agttcaatca acgtagatga
     5461 gacaaacact caaatgatcg agtctcaaag caatcgaaaa acttccgaag tctttcttcc
     5521 gcttcatgtt acatggcacc attcgggaat gatccaagaa accaacagtg aaaatctcgt
     5581 gtaccgattg caattgatca tcgataaagc aagatttgga aagctttctg aagtagtttc
     5641 aggagacaaa tcacagtttg aacaggtgaa aaatctgaac tctggttttg agttggccta
     5701 taatgcgcct tttttgctaa gagaatacaa ggaagcattt gatttagatt tagatgtgtc
     5761 gaacttggca aaagaaagcg aaaatatgta ttttactaga atacaattcg atcaaggaaa
     5821 taaaaaaatc cgatttttga attattcgaa ccatctcgta tatgaagcaa atatttcgtt
     5881 gaattggaca gatatcaata aagaattaac agcaaaagac acaaaatgga cagaaatgac
     5941 aacagaacca tcgacgatcg acacgcaata tgctacgaaa cattcgatca aactgaagaa
     6001 gtatagttat atcctgtcac agcaacccta tacacttttt agaaatgctt ttttccagaa
     6061 accagatagc gtgaaaaata atgatgaaag caatgaattg attttttacg gtgggaatga
     6121 aacaatgtct atccattcgg aaacgcaagt agttgatttt cgtggagagc tgccagagaa
     6181 aaaagaaaat gctagtgtat tttctgaaag tttcccctat attagcaaat taggcagttc
     6241 tttagggaac ctcagatatt ttgaccgcag tgacaatacg atcgattatc ggatattcgt
     6301 ggaaggcttc ccggtttttg ggacagacag cagaggacag attggtgtgg aagttggcgg
     6361 tgaaacgaat ggttctgtgc aagtgaatat ccaaaccagt ttgaatacga ttcaagtgcc
     6421 gataccttca gacgaagaag tagaacttgc aagtacgaat gaaatcatcc agcagttaaa
     6481 agctaaggga gcagatagca acaagattgg ttcagtgatc gttggctata catggcaaaa
     6541 tgtcaaagaa acgaatcgtg tcgttgatct cttacctgaa tggtatgtga aatataaaga
     6601 taactggtat tcagctaacg accttctgga aaaattacct ggattggagg cgaattaatt
     6661 ggatttcaaa aaaatcgaat ggatattttt cgtcgctttt ttagggttga atgtgttttt
     6721 attcagcagt tatcatgacg cggtatacca agaaaacaat gtcattcgct ctaatcagac
     6781 tgaatcaatc caacaacgtc tggctagcga cgatatcact tacacgggga agatttcgtc
     6841 tgagaacaag caaggctatt atcttagcgc ggagcagacc aatcttagca cttcattagc
     6901 gaattatcgc aaggctggta aaagcggctt gctggcaagt ggtgtgtttt tagacggtga
     6961 tgttttgacg aaatctttat ccagttcaga tgctgaaaag aacgtgatcg atccagataa
     7021 aattagtagt tccttggaag attttttatc taacaaagac aatattttat tcggtaatga
     7081 ttatgtgtac acgaaaaact tttctcattt agaagaagaa ccactagaaa tccaagcaag
     7141 ccaagagtat gaaggcatcc cattcaatga tgatacagca aaaatcgttc tttctttaga
     7201 aaaaaacgat aatgattata gcatcacgaa atacatgcag acgcatttat ctgatatcga
     7261 gcagctgcgc gaaaagacgg aactgtatac ggaagaggaa gcaatcaata cattgtacat
     7321 caataacaaa ataccaagag gatcaaaaat tttatggcgt cagctagctt attcctgtat
     7381 tctgaaagtc cgggaaaaaa atgtgtatgt tcctgtttgg catgtagcga tcgaaacaag
     7441 cgataaagtg gtccaaattg agagcgtcaa cgctttcagc aatacgatcg tgacaaacaa
     7501 tactgtacca aaagtggaag atcaataaaa aagcgagtat aatgaaaaaa gtttacaaag
     7561 taagaaggga agtaggagtc aaatggctga agaaaaacca gcttttaaaa tcagtatcct
     7621 agcaagtgga agttcgggta attcacttta tatcgaaagt gaaaaaaaac ggcttttggt
     7681 cgatgcagga cttagcggta aaaaaatcac ttccttgatg gctcaaattg atcggacacc
     7741 ggaccaatta gatggaatac ttgtgacgca tgaacatcga gatcatatac atggcgtggg
     7801 tgtcttggct cgtaagtatc atttggatgt ttttgccaat cagcagactt gggaagcgat
     7861 ggaaccactg attggaaatg taccagtgga acaaaaacat ctatttgaga tggggaaagt
     7921 cagaacattc ggcgatatcg atattgagag tttcggcgtc tcccatgacg cagcggctcc
     7981 gcaattttat cgtttccata aagaaaatcg gtcattcgtc atgttgacgg acaccggata
     8041 ttgcagtgat tatatgcgag gtgtcattga aaatgccgat ggttatttga tggaaagtaa
     8101 tcatgatctt gagatgttac gcatgggacc ttatccgtgg aatttaaaac agcgtatttt
     8161 aggagataga ggacatcttt ccaacgaaga cggtgcactt gtcatgactg atgtgatcgg
     8221 taatcggacc aaacgaatct atcttggcca tcttagcaag gaaaacaata tgaaagaact
     8281 tgctcattta acaatggaaa atgttttgaa agaaaaagat tttggcgttg gacatgattt
     8341 tgaaatctat gatacagatc cagatgaagc aactgagcta ttttcgattt aaacagttca
     8401 atcaataaaa atataaaaag cagaagataa aatcaatcag ggttggtttt atcttctatt
     8461 tttatttttt tagcatatta aaaaaataac cgtattatcg actgactaca ctgatgtgct
     8521 gcttttcatg tttctcatcc agtatctcat ctactaatgc aatcgcgtaa tctgccatgc
     8581 tgatcgtact gtcgctagca gcgtttcttt gtaaataatc atcacttaac aaataagtac
     8641 ctgtacgttt gccatgtgga tcaaataaag aagctggact gatataagtc caatgtagat
     8701 ttttagattg tttcaatttt aacaatcctt cgtacatatt gaaagctgtt ggatagaagg
     8761 gtgcattttt aggtacttgg tcaatcatac gagtcgtctt ttctgtatct aagaataacg
     8821 aagaggcacc cccaacgaca attaagcgtg tatcggttcc ttcaagcatc tcaatcaaat
     8881 gggacaaaga agtttgatgc atttcttctt ttcctttggg agcgttgaaa gcatcaacga
     8941 caacatcaaa agatgcaaga tcagtagttg tcaactcaaa taaatccttt tgtataaacg
     9001 gaacatctga tggcaatttt tctggatgac gcacgattgc cgtcacgtct aaatctctgg
     9061 acaatgcttc gtctaaaata aatgaacctg catggccagt agctccaatg agtgcaattt
     9121 tcataaaaaa cctccttttt tattcctgtc cattgtaagc agttgctgaa tattcgtcaa
     9181 agaatccgat ttttgaaaag ctcagtgcgc attcctttca tggttcgtga tcattgggta
     9241 tagttaaatt aagaaaactg attgataaaa atagaagttg aaggagataa gaaatgaaag
     9301 agtggttaga tcgtgccttt ttaccaatga taacatctgt tgtgaatctt agacccttaa
     9361 ctgctttgcg taatggaatg acttacacga tcccaataat tattgttggc tcgctgtttt
     9421 tcttatttgc tcattttcct gtggattaca tcgctcagtg gatagacaaa acaggatttt
     9481 ctatgtattt tacacaagta taccaggcta catttgatgt gatagccttt tggatcgttt
     9541 ttgccattgc atacgcttat gcaaaagaag aagacatcga tcgcttacca gtcggcataa
     9601 cagccttgtc tgcttatcta ctgcttattc agccactaaa tactcagata gatactgtct
     9661 ggacaggtgc tgaagggata ctaggagcag ttacaactgg gctcatcatc ggatggggct
     9721 atgcacgttt agtaagactt gcgaaagggt ggaagcaaag agagaagata cctgaaaatg
     9781 tttggcagtc ttttacttca ttgattccaa tcattgttat agtcactttt acgcttatcg
     9841 gttcagcggt ttttcaacat ttgttcagaa tgactgttgt tcaaggagta tggacatatg
     9901 tccagcttcc cattcaatcg ttcatagaca cgttgtttgg tgtgctttta ttaggttttt
     9961 ttgttccgtt tttatggtta tttggtcttc atggatctgc gatggtcaat gggttggtta
    10021 gtcccgtctt gcaagcgaat tcattagcaa atgcagaaat cttagcctca ggacaagagt
    10081 tgacggtggc aaatggcgga catatcgtta ctcagcaatt tctcgatcag ttcatgacgg
    10141 ttacgggtgc tggattgacg ttaggggctg tattttttat gacggttttt gcgaaatcga
    10201 agaaatacag agaattagga aatcttgctc ttttaccagc ctttttcaac atcaatgaat
    10261 caattatttt ttccacaccg atcgtgatga acccgatgat ggctgttcct tttattttcg
    10321 caccggtcgt ttcaggattg attacctatt ccgctttata ttttggattc gttcctttat
    10381 ttacagcagt ccaagtccct tggacaacac cgccgatcct ctcgggtttc ttagtaggcg
    10441 gagtgcctgc agcgatttta caaggtatcg tgttgtcaat tagttttttc atttatttcc
    10501 catttttgaa aaaaatagat tctgcaaact gcaaaaagga aagacagaca aaaaatgatg
    10561 gagcagcaga aaagatgaac gactaaaaaa caaaaatact cctgtcgctt tagattggga
    10621 aaatcaccaa tctaggatga caggagtatt tatagtcatg tttatactag ccctttttgg
    10681 acagctaatt gaacaagaac aactaatgtg ttcattccta cgtgtgtgat catcgatgtc
    10741 cagatccgac cagttgcttt gtactctagg ctgaagaaga aacctaggaa aaagtagatt
    10801 agcagatgac catcattatg agcaaacgca aacagcaaag agctgatcac tgctccgacc
    10861 caaaaatttg aatatcgact gatgatcccc agtatactgc ggcggaatac gaactcttcc
    10921 atgatgggtc cagcaacagt tgtcgccaaa ataaaagcgg gtgcttccat gatcatctgg
    10981 atgatatttt gtgtattctc ggaaggggtt gctttgccaa acaatacaga ttctaacatg
    11041 acagcgatgc tttgcagaat caatgcgaca aagattccaa ccaatccata aataacaatc
    11101 tttggagcag aagctttttt taccgctgat tcgatcgcta agggttcacg ttgcttaaaa
    11161 taaaaaatag ctaggacaac ggcgcctagt atatacatga ctgcagttgc tgtgatcaat
    11221 tcagacgtag attttacaag tcctatcgaa gctaggatca agggtgagat aaaaattaga
    11281 ccatagcaaa aaatgctgag gaagctatat ttttttaaag acatgtaatt cctcctttta
    11341 agggataaca agcatgcaag gcaagcttat atcgattgat ttcaattata tcataatttc
    11401 aataagcagc tgtcagaata aaatttcttt tctttgattt aaaagcttgc aaaaaagagg
    11461 gagaatgagt attattaaaa atgtaggtta gcactcgaag gttaagagtg ctaacagatt
    11521 aaaaaaagag ttatcggagg gatttatcgt gttaaaacca ttaggtgatc gcgtcattat
    11581 cgaagtcgca aaagaagaag aaaaaactgt cggaggaatc gttttagctt ctgctgctaa
    11641 agagaaaccc caaactggga cagttgttgc agtcggtgaa ggccgtttgt tagaaaacgg
    11701 tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt atgttcgaaa aatacgccgg
    11761 tacagaagta aaatacgaag gaaaagaata tctgatcgtc gcaggaaaag atatcatggc
    11821 gattgttgag taaatttgga agataaaaaa ataatcaaaa ataatcaatg aggtgaatca
    11881 atcatggcaa aagaattgaa atttgcagaa gacgcacgcg cagctatgtt acgcggggta
    11941 gataaattag cagatacagt gaaagtaaca ttaggaccta aaggccgaaa tgtcgtacta
    12001 gaaaaatctt acggctctcc attgatcaca aatgatggtg tgacgatcgc aaaagaaatc
    12061 gaattagaag atcattttga aaacatgggt gcaaaacttg tatcagaagt agcatcaaaa
    12121 acaaatgata ttgcaggaga cggtacaacg acagcaactg ttctgacaca agcaatcgtt
    12181 cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc taggcattcg tcgcgggatc
    12241 gaattggcaa caaaagcagc agtcgaagaa ttgcataata tctcaactgt tgttgattca
    12301 aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt ctgataaagt tggacatttg
    12361 attgctgatg caatggaaaa agttggaaac gacggtgtca tcacaatcga agaatcaaaa
    12421 gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt ttgatcgtgg ttacttatca
    12481 caatatatgg taacagataa cgacaaaatg gaagctgttc tagaaaaccc atatatcttg
    12541 atcacagaca aaaaaatctc taacatccaa gatattctgc cattgttaga acaaatcttg
    12601 caacaatcac gtccattgtt gattattgct gacgatgtag atggtgaagc attgccaact
    12661 cttgtattga acaaaatccg tggaacattc aacgtagtag ctgtaaaagc tcctggtttc
    12721 ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt tgacaggtgg tacagtaatt
    12781 actgacgatc taggtttaga attgaaagat gcaacaatcg aaaacttagg aaatgcttct
    12841 aaagttgttg tagataaaga caatacaacg atcgtagaag gttctggaga aaaagaagca
    12901 atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg aaacaacttc tgattttgac
    12961 cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg gtgtagctgt tgttaaagtt
    13021 ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac gcattgaaga tgcattgaat
    13081 gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg gtggtacagc tctagtgaac
    13141 gttatcagca aagtttctgc tgtagaagca gaaggagacg tggcaacagg tatcaagatc
    13201 gttgttcgtg ctttagaaga accaattcgt caaatcgctg aaaacgctgg ttatgaagga
    13261 tcagtgattg ttgataaatt gaagaacgtg gaactaggca ctggtttcaa tgctgctact
    13321 ggagaatggg taaacatggt tgaagcaggt atcgtagacc caactaaagt aacacgttct
    13381 gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa cgacagaagc agtcgttgca
    13441 gataaaccag aaccagcagc gccagcagct cctgcaatgg atccatcaat gatgggcggc
    13501 atgatgtagt aattaagaaa gaaccctatt ttaacagggt tctttcttct ttttttgttt
    13561 tattttaagc aaaaggggca aagaaggggc aaaaatatca aaggctattt agtttatcaa
    13621 ttacttgttg tttggctctt tttgttacat ggttataaat agacagagtt gtgtttgcat
    13681 cagaatgccc tacacgctcc ataatggctt taagaggtac gcctaattca gataatagtg
    13741 aaacgtggct atgtctgaat atatgtgagg atagactttt ttctaattcc aactcttcct
    13801 ctactttatg gagtatagcg ttaaatgagt gcagcgcaag cggagtgcca cttgtagata
    13861 taaatatata ttgatcggga tctgtgggtc tacctgcaag aatattgtca gctatcacac
    13921 tttcaatcaa ttcttttgca cgattgggta attgcacttc acgttgcgaa taagtatttt
    13981 ttggagttgt ttttatagca ttatccattt tcacagatgt gtaatctaag gtcccattaa
    14041 tggaaatttt cccatcctca tagtccttca tttgcaaagc tagcaattcc ccatatctca
    14101 aaccagttaa atatagaaac tcagctatta tgccgtgtag ttttctgcga ggattggagt
    14161 atagctgttt cagtatttga tcaatttctt ctttctccag atatttttta ttcatagaaa
    14221 gccttctttt ttcttcttcc acttttttag gatggatttt aactgctagc gcagggtttc
    14281 tttgaatgta ttttctatcg attgcatagt ttagcataac agatagagtt gtttttgttt
    14341 gttttgtgta gttcagtgag aggtcaccaa acgtatacat atcttcaatt atcttattaa
    14401 tgagtgtctc atcaatgttt ctaacgatcg tatcatcgct tatgtgctta gaaacatgtt
    14461 tcatcatcat tggaaccttc aaatagctag tacgtttaac atgctgctta taatattcat
    14521 accattcttt atacagctca ccaaaagtga tatctgattt attgtaatct tcgagtgctt
    14581 ctttaatttt tttatctaga attttctgag cttttttcca cgcttgtggt gaattacttg
    14641 taagtgttgt agattttttt cgtgtttttt ctgtataagg atctacatat ctttcaataa
    14701 acttgaattg gccatctttg gtctgttcaa cccacacttt taacatctcc tatcatttgc
    14761 tataataggc ataacaaata gacctatata ggtttgtttt ctaaaagcac gctcttactc
    14821 tggacggtgg ggcgtgtttt ttatttatta tacagttgct ttaaaaatag ctgctttttc
    14881 aaatgatact aaaggagaga agtggatttc tatttctccc ttttggttta acgtgaaatg
    14941 agcagttaca tccatttgtt ttcctggagc aacagatccc attgtatttt cgttggcata
    15001 tgtctctgat tttttgtcat ctggtccata tacttccaca tctgtaccta cagggatatc
    15061 tgaatcgcca tcatttttta cggtataagt aatttttact acttgtgcag gttggttttc
    15121 ttcaaattga tttctttcat cagttagttc tacactattt agtgtatatt cagcatcacc
    15181 aacttttaca gtgtcaccaa tcttatagaa ggtatcgctt ttttcttctt tagaagatga
    15241 agcagaggtt gaagatttag ttactttttc gccaccatta tcacttgctt tattactatc
    15301 tgatccacca tttaaagcag aaccaataat tataattaaa attactgcta atacccaaaa
    15361 ccatactttt ttgtaaaaag gtttactaac tttatacatt tttccgtctt gacccataac
    15421 tttttttgcc atttaaatat tcctcacttc ttgttataat atatttgcga tctcagaaat
    15481 gaggtatgag tccgtgtgcc agcacgggct tttttaatgt tttgctgaaa tcggtttttt
    15541 taataattct tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata
    15601 gcattggcag acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat
    15661 aaatttttcg agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa
    15721 taaaattaga atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga
    15781 agtatcgtac aaaactgaat attcaaaatg agaagcggtg aaatgcgcaa gttcatgaga
    15841 taaatgaaag gcttctgcag tttcgccata tagattttca ttcaaaaaaa tgattctagg
    15901 cttcggataa tagaagccag gctctttcat ttccatgtaa actactttca aattgtattc
    15961 gctcaacatt tctttcaatt tcaaatacat acaacccatc actccaacta ttcattttcc
    16021 tctaaagctt tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca
    16081 tcaaaagaaa aaacagtatc gttttctgat aaatccacat gtttaggggt ttctctttct
    16141 tctctaccta gaaggtagtc tacagagact ccaaagtaat cagctaaccg agctaactca
    16201 atagaattag gagtttgtct tttccaactt cctaaatatc cattcgaata cccgaaattc
    16261 atttcgagct gtctaatgga taatcctttt tcttttgcta actcctttat tatttcgtaa
    16321 gtattcattg ataaatcaac ctttctgaat gcttacaaaa aaagtttaga taaataatgt
    16381 aaaaatagtt gacttatata cattattaat ctatactatg ttttgtaaac aagtttaaca
    16441 actaaaaaga caacaaaaaa tactattgat taataaatgc caaccgccaa gaaagctttt
    16501 taaatcaaag tttatatgtc ttatttaact atgcatttag tatagattat taatctaata
    16561 aagtcaacgc agttttttaa aaagttgtta aatttgttta cgaatataaa agaaaggaga
    16621 gaagaatatg gaaaaaacag tctcggcaaa aatcgaagat ttgaaattag acatactgaa
    16681 acaagcaaaa gtggcgatgg aacacgcggt agataaagaa gactctgcca tggttgcagc
    16741 catagcagag attttagctc acgtttagtc atcgttttta cggtcttctg gaagctgacc
    16801 atagataaca gcataatgaa tatctaaata agcttgaaca atttcttttg gcgaaattga
    16861 ttcgccttta gtagatactt gtgattcgtg atttagccat gcaacaacta aatccgcagc
    16921 gatttcaaac ggcgttacac tttgtgtcag ttgccataaa gaatttcaca atatgtatgg
    16981 tcgtggtaat aataccaaag agcaatttga ggaatttatg gaaattaaaa aatacaaaaa
    17041 atcatttatt aaaggaggct aaacaatgaa caaggaaatg agccaaacaa tcaaggtaca
    17101 aaaaatgata gatgatctaa cacatggaat tgatagccaa gccgataaaa tcatcaagga
    17161 attgcaagga caaaaagtta aggatgcaaa aatgcttctc aagactatca actttgaaat
    17221 gaatccaaca aaaagaaagc tcgccgatgt gttggaagaa aagttggctt ccgctatcaa
    17281 cgagcaagaa ttactatttg aaaccgatac ttttaacagc tgatgtttta tagatgacag
    17341 tttcatccga atctatatca tcgccgagag aaaagaatgt tgcttttgac aaaaagccga
    17401 taagacctac aagcggtctt gatgttgcaa gagtagatac cccaatatca ccttgcaagg
    17461 tattcacgta aatttgagaa gaatagaaca ttccatcttt ttcttcgata tttgacgcat
    17521 catcgtatcc aacgaccaaa gtatcggcaa atattttgat tttttctcca tcaagcatag
    17581 taattacaaa ttctggtttc ataaattttc acctccttaa caattatttc agcctgtcac
    17641 actgataagg aaattatacc aaagaaagga atgagaaata tgaatacacc acaaattttt
    17701 aatttcgagc aaaacgaagt tcgaactatt ttagtaaatg atgaaccata ttttgtagga
    17761 aaagatgtag cagacgttct aggatattcg aatccgcaaa aagctattcg agatcatgtg
    17821 gacttggagg ataagacgca gaacgattcg ttcaccgtca atggaacagc agttgttttg
    17881 attaacgagt caggccttta cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa
    17941 tttaaacgtt gggtaacgag tgaagtcctt ccaacaatta gaaaaacagg tagctattca
    18001 aacgtacctc aaagttttgc acaagcattg cgtttagcag cggatttaga agaaaagaac
    18061 caattactcg aacaacaaat tgccgagtac gaaccaaaga ttagctactt agatacgatt
    18121 ctttcatcga cagatacggt agctacttct caaattgcag ctgattacgg aatgtcggca
    18181 attgctctaa acaaattgct aaacgagtta ggtgttcagc ataaagttag cggacaatgg
    18241 atactttacc gaaaacatat gaaccaagga tacacaaaat cgcacacaag tgagataccg
    18301 aaagccgatg gcggcactaa agttgtaatg aataccaaat ggacacagaa agggcgagtg
    18361 tttatttaca acttattaat cgcagagggc tattaccctc aaatggattt agaggaaatt
    18421 ggttagaaag gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa
    18481 aaatcaaaga atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat
    18541 ttggaattag caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag
    18601 caaacagaat tatagattcg attatcacta tgtacgaatt gtagagggag gtaaagtcgt
    18661 tggaagataa aatcattctt acaaaatcag agcttcaaga aatgttaaac atcgagtatg
    18721 gaacgataat ttttaggagg gataataagt gaaaaaacca acactttcag agctgataga
    18781 agctgctgag aaggcagtaa aaccagacga ctggtaccga caaagtttaa tcttggagaa
    18841 gttccacggc atgtcaaaaa ctactttagt tgaatactgc aaggaaatgg aaacaattcc
    18901 tgaatttgca gaaggaatac ttcgtccagg tcactcaacc acatttattc attaccatac
    18961 ttttatttgg tttttaaagt ggaaagacgc aaacaaatat cgtgtaaaaa gtttatcgcc
    19021 ttcagatgtt ttgaaggagg caagttaatg ggcaaattca acagagcact agtattcagc
    19081 gcaccgctaa tcatctacgc tttaggcctc tggggaagca gacaagcgtt gatagggaca
    19141 atcgtttaca tggtttggat ttttatgggg cttgatgaag ctgagcacag agcgaaaaaa
    19201 gccagtcggg agggactgac taaaatatga tttttttatt tttgttcctt tgggctgcta
    19261 ttgcgtactt catccagctc cttgttgtca aagttgctct tatttctttt aggaagaaca
    19321 aaagaaaata gcgctataaa tgaaagataa gtagccgcta cattaaactg ttcttttgaa
    19381 aaagttacag tcatcggaat gataattgat attgaggaag taatcttaat taaaaatata
    19441 gcaatatttt caacatcgcg ttggttttta tttaacattg tccaacatgt tttagcaaca
    19501 ataaaaataa ttacacatga tattagcaca cagactgaaa aaagttttag aaatgcgatg
    19561 tagtcagtgt tattgaactc cttcaaaata ctatttttat tcaatgtaat aaaaggaatt
    19621 tttttaattg aagacaggaa agataaggta ataaacagaa ttatatatat ggttaaagtc
    19681 gcagtaagaa tgtaattgag ctttttcctc atagaatcac ctcactttct aacgaaaatt
    19741 ataccaaaaa ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct
    19801 gtaactacga gcttacaagt ggcagaaagt ttcaaaaaga aacataagca tgtactagaa
    19861 gcaattgaat caataaaaag atcggtcgaa aattcggcca atgttgaaga tgggtccaat
    19921 tttggacaga tgtttgtgga agggaacgag ccagactcat acggaagaag tcggagagtt
    19981 tatttcatga atagagacgg attttccttg ctagctatgg gattcactgg aagtaaagca
    20041 ataaatttca aactaaaatt tattgaagct ttcaacgaaa tggaagatgt tattcggaag
    20101 aatactgttc ctcaaacaat tgaagacatg atgatctatc aattagaaga aatgaaagat
    20161 gttaaaaaag atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt
    20221 gaaattaagc aaaaaggaaa tatgaaagtt atggaagttc tagggggaaa agaaagccga
    20281 gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc
    20341 ttttcaatcc caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt
    20401 attgaaatgt ggttaccaga aactgcgatc cgcatggaaa tcgatcaact gaacagacaa
    20461 caaagacttt tcggtgatga aaatgaatag agctgaagcg ctaagaatag ggacggtaat
    20521 tgctaatcgc tggtggagac acaataaacc aaacatccta agccaacaac atatcgaaaa
    20581 gcaaaaagca tggcaacaaa taaaaaagtg actcagccgg caagcataga gtcacaaaac
    20641 atatctaagg agaattttag catatgaata aagaactttc cactttagat caatatttga
    20701 ctgattctga atggggcaag tcgaatatca aggaaacaaa taatcgaaaa atcagacgaa
    20761 atcttttgac gaacgaagaa ctatcatgtg atcaagatga cttgggaaat tttgtgagta
    20821 tttgggatca tgtttacctt atccatctat caaaacattc aaataaacct gaatacatct
    20881 atgtcatcga agatggcttg attgatgcgc tagaagagta caacagagat aacttgattg
    20941 atatctctta ttacggatca ggtaagaaat acattgctga aatggaggca gaatttgatg
    21001 agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt
    21061 ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa
    21121 aagaatgatc cacaggcaac ggtaactatt aatgagtata aacactacag ggttgtttct
    21181 ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaacaggg
    21241 acttatgtat ctgtctcagt aacggttaaa ggacatacgg aaacaga
//
LOCUS       DACDKL010000030        20548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDKL010000030 DACDKL010000000
VERSION     DACDKL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.030"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            27..395
                     /locus_tag="IV329_000881"
     CDS             27..395
                     /locus_tag="IV329_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8940085.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            565..996
                     /gene="mraZ"
                     /locus_tag="IV329_000882"
     CDS             565..996
                     /gene="mraZ"
                     /locus_tag="IV329_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8940086.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1017..1976
                     /gene="rsmH"
                     /locus_tag="IV329_000883"
     CDS             1017..1976
                     /gene="rsmH"
                     /locus_tag="IV329_000883"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8940087.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1981..2382
                     /gene="ftsL"
                     /locus_tag="IV329_000884"
     CDS             1981..2382
                     /gene="ftsL"
                     /locus_tag="IV329_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8940088.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2383..4575
                     /locus_tag="IV329_000885"
     CDS             2383..4575
                     /locus_tag="IV329_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8940089.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4601..5563
                     /locus_tag="IV329_000886"
     CDS             4601..5563
                     /locus_tag="IV329_000886"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8940090.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5577..6938
                     /locus_tag="IV329_000887"
     CDS             5577..6938
                     /locus_tag="IV329_000887"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8940091.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6964..8052
                     /gene="murG"
                     /locus_tag="IV329_000888"
     CDS             6964..8052
                     /gene="murG"
                     /locus_tag="IV329_000888"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8940092.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8067..9287
                     /locus_tag="IV329_000889"
     CDS             8067..9287
                     /locus_tag="IV329_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8940093.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9443..10768
                     /gene="ftsA"
                     /locus_tag="IV329_000890"
     CDS             9443..10768
                     /gene="ftsA"
                     /locus_tag="IV329_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8940094.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10791..12032
                     /gene="ftsZ"
                     /locus_tag="IV329_000891"
     CDS             10791..12032
                     /gene="ftsZ"
                     /locus_tag="IV329_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8940095.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12049..12726
                     /locus_tag="IV329_000892"
     CDS             12049..12726
                     /locus_tag="IV329_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8940096.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12740..13339
                     /locus_tag="IV329_000893"
     CDS             12740..13339
                     /locus_tag="IV329_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8940097.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13354..13641
                     /locus_tag="IV329_000894"
     CDS             13354..13641
                     /locus_tag="IV329_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8940098.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13690..14472
                     /locus_tag="IV329_000895"
     CDS             13690..14472
                     /locus_tag="IV329_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8940099.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14493..15200
                     /locus_tag="IV329_000896"
     CDS             14493..15200
                     /locus_tag="IV329_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8940100.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15462..18260
                     /gene="ileS"
                     /locus_tag="IV329_000897"
     CDS             15462..18260
                     /gene="ileS"
                     /locus_tag="IV329_000897"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8940101.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18309..19835)
                     /locus_tag="IV329_000898"
     CDS             complement(18309..19835)
                     /locus_tag="IV329_000898"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8940102.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19850..20497)
                     /locus_tag="IV329_000899"
     CDS             complement(19850..20497)
                     /locus_tag="IV329_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8940103.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6936 a         3562 c         4402 g         5648 t
ORIGIN      
        1 caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc ttgttttggt
       61 atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa
      121 atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc
      181 atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt atttctgttt
      241 tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa attatttatc
      301 cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg
      361 tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt
      421 cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata aaaaaagttg
      481 ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac taattttagt
      541 tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa tattgatgca
      601 aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg
      661 acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga aaatctggaa
      721 gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt gcgattcttc
      781 tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat acccagcacg
      841 ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt
      901 gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga gaactttgac
      961 gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc tgactaatga
     1021 ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga ttgaacatca
     1081 aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt gaatatttat
     1141 taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg
     1201 atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca tttatcaaag
     1261 cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta gacggcattt
     1321 tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga tttagttatc
     1381 atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc gcttatcacg
     1441 taattaatga atattcctac catgaattag taaagatttt cttccgctat ggcgaggaga
     1501 aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa
     1561 caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca agaagaaaag
     1621 gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat gacgaactcg
     1681 gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat ggaagaatca
     1741 gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt aaagaatata
     1801 gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc caaccagaac
     1861 tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca gaaaacaatc
     1921 gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag gagtagaatg
     1981 atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt ggtggatcag
     2041 ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg
     2101 gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt
     2161 gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag cggagctgaa
     2221 aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa aacgagttta
     2281 gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc agaagaaaaa
     2341 ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct taaaaataaa
     2401 attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt
     2461 atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg tttctcgtat
     2521 atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa acagttgtac
     2581 caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg
     2641 ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac atatacctct
     2701 ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga aatcctgcat
     2761 aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa
     2821 gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga aaccaagcaa
     2881 aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt cgaccatcag
     2941 gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc agttccagac
     3001 aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa tgatatcttg
     3061 agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc tctgccaggt
     3121 acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac tttagacagc
     3181 agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata tcagccagaa
     3241 gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat gggacaacga
     3301 cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat ctggcgtaat
     3361 ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac tactgcagct
     3421 gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa aatccaagta
     3481 gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa
     3541 gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt aggcggacga
     3601 tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg attagatgat
     3661 gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag tgcttatgga
     3721 caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat tgcgaataac
     3781 ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac tggagaagag
     3841 cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac agaaaaagtc
     3901 cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat
     3961 agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc ttctgataca
     4021 gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat ggttccttct
     4081 gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata tgatcgaatg
     4141 gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa agaaactgaa
     4201 gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta tcgtaatctt
     4261 gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt
     4321 aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat ctctggagaa
     4381 aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg atggtcaaaa
     4441 gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga tggagatggt
     4501 tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa acttagtttt
     4561 actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga cacaagcatt
     4621 gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat ttattggtta
     4681 ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag
     4741 caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta ttttgacagg
     4801 aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct tgctttttgt
     4861 attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat tcaagaaaag
     4921 aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg gaatcatctt
     4981 ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg gactagatct
     5041 acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat tttcaaatgc
     5101 agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta tctcttttgc
     5161 agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca tttgcttaag
     5221 tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca agatttttat
     5281 gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta taatgctaaa
     5341 ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag caagtgtgat
     5401 gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt ctccaattca
     5461 tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt tttggttgat
     5521 cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga cgggaaatga
     5581 aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca aaaagcggag
     5641 taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat gatggaaaac
     5701 cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa gtaataacag
     5761 gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa aatccaggga
     5821 ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg gtaattacgg
     5881 aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca ggaacgaacg
     5941 gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca gatcaaggaa
     6001 ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat
     6061 cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc acagatttcc
     6121 gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat catggtacaa
     6181 gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa aaagattatc
     6241 tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca aaagcacgcg
     6301 tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac gattactcaa
     6361 tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca ggtaagcata
     6421 atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata tcaaatgaag
     6481 ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag
     6541 aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta gcgactaaaa
     6601 tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga ttagacagag
     6661 gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg gtcgtatttg
     6721 gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag acaatagtaa
     6781 cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca gatggtgatg
     6841 tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt gaaactagag
     6901 gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag cgagcgataa
     6961 aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc agctcttgcc
     7021 tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa
     7081 cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac cttggagatt
     7141 caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt gtttcttaag
     7201 agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt tatcggtact
     7261 ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat tccaacagtt
     7321 atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg atatgtggat
     7381 cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa agcagttctt
     7441 gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat tttgaaaaca
     7501 tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca aggagcactt
     7561 aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc ttaccaagtt
     7621 ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa tcctgaaata
     7681 caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc ttccagtgat
     7741 ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct ggggttaccg
     7801 gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa tgcaatgagt
     7861 cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg tgaatcacta
     7921 agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat gtgccgagct
     7981 tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt aaaacaaata
     8041 atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc
     8101 atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga aaatatctgg
     8161 cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta gatacacccc
     8221 tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa agccagtccg
     8281 agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga ccatataacg
     8341 gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc
     8401 ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc tattatgttt
     8461 ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc aacagccaag
     8521 aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag tattttcatc
     8581 gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca gcaaatatac
     8641 atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag attgctttga
     8701 tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta gatgaaaaag
     8761 tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct gcaaaaatca
     8821 aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca atttctgaaa
     8881 tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg aatgacggca
     8941 atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat tcgcaagtag
     9001 ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt
     9061 acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact gaaagtgaag
     9121 aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac
     9181 ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa
     9241 tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa aacaacttta
     9301 aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga agcagtgaat
     9361 atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta tatggaatag
     9421 taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg
     9481 aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga atattattgg
     9541 agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata tcgataaaac
     9601 ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta tccagatcaa
     9661 aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc agggaatgat
     9721 tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg tggcttcagc
     9781 tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt
     9841 tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg taagattaga
     9901 aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac gtaaatgcgt
     9961 ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc tgactgaatc
    10021 aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg gtggcggcca
    10081 aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg accaagaagg
    10141 tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta acaatgcaga
    10201 agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag atgaagaatt
    10261 tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct acttgtcaga
    10321 aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat tggaccaaat
    10381 cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg
    10441 tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca
    10501 catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt attcagcaaa
    10561 cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg
    10621 tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag cacctgaaga
    10681 aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa
    10741 gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat atggaattct
    10801 caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc ggtggtggcg
    10861 gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa ttcattacag
    10921 ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc caattaggcc
    10981 ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa aaagcggcag
    11041 aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt ttcatcactg
    11101 caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag
    11161 aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac
    11221 gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat acattattaa
    11281 ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg cttgaagctt
    11341 tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat ttgatcacag
    11401 cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa aaccaaggta
    11461 cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa
    11521 aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa gtacttctta
    11581 acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca gatatcgtag
    11641 caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat gaagaaatgg
    11701 gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca
    11761 agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag actgtattag
    11821 atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc gattgggata
    11881 tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac aatatcgaaa
    11941 aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa
    12001 atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat gattgctgac
    12061 aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca tcgtgatccg
    12121 aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg
    12181 gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa
    12241 caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt acagcgcaga
    12301 aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag tttaagatta
    12361 gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga agtaaatgtt
    12421 tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt tgtggaatct
    12481 ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc taaagaagct
    12541 tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga tcttgttcaa
    12601 gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag tcaagacttt
    12661 caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct attcaaagac
    12721 gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta attttttcgg
    12781 attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa aaaaggctgt
    12841 acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat ctgctgcgaa
    12901 tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa gtattcagcc
    12961 ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg tagcgaaaga
    13021 aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa aaatcactgt
    13081 aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca ttgctggaga
    13141 agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa tcgttgattt
    13201 tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag atgagatttt
    13261 tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt ttgcagatgc
    13321 gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact attacttcat
    13381 ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc attgctctct
    13441 tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat ctgcgaaccg
    13501 tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt cacaatttta
    13561 ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt tatatggata
    13621 actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa tagataggag
    13681 gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg tacatttatc
    13741 gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta tctgaccgaa
    13801 tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag cagtgatttg
    13861 cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt gattttcccc
    13921 gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat ccattatcct
    13981 agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg aacggggatc
    14041 aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt atttattgca
    14101 aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc
    14161 ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac acaagaaaga
    14221 ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa tatttccaga
    14281 cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac agaaaataca
    14341 agacctgatt ttgtacttga actattagat attatttcag tcagaggtta tggcagagtc
    14401 cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact gttcggtgta
    14461 ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta gatatccaaa
    14521 ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat gatttcttag
    14581 atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag
    14641 tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct ttgaaccaat
    14701 caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc aaggaatccg
    14761 aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac gctgaaaaac
    14821 gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa
    14881 cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa acacgagtat
    14941 tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa agtccggaat
    15001 gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa gtgatcaaag
    15061 aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca gacacaggag
    15121 tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg atcgtgcctg
    15181 gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc gagccgatga
    15241 gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga cctgcttttc
    15301 cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag tttgcactag
    15361 atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc
    15421 ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg aaagaaacct
    15481 tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga gaaggcgagt
    15541 ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg aacgaaggaa
    15601 aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc catttaggac
    15661 attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg tctggctatc
    15721 gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa gtattggcta
    15781 ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat
    15841 atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga gttgcaggag
    15901 attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa gtacgcgtat
    15961 ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt tactggtctc
    16021 cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta aaatctccat
    16081 ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa gatacagctt
    16141 ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc
    16201 caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt
    16261 tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta aaagaatttg
    16321 ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt gaatcacttg
    16381 tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat actgctccag
    16441 gacatgggga agatgactat atcgctggta gcaaatataa actatctgta gtgtcacctg
    16501 tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc ttttatgaca
    16561 aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg aagctagatt
    16621 tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa
    16681 ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat gaagtagaaa
    16741 aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg
    16801 gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt ttctatgctg
    16861 aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg
    16921 aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct gaaggattta
    16981 cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg gatgtctggt
    17041 ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta tcattcccag
    17101 cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca agtatcacga
    17161 ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa ggatttgctt
    17221 tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca ccagaaaaag
    17281 tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta gattatgaag
    17341 cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat cgtaaaatca
    17401 gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa aaagatactg
    17461 tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg aaccaagtaa
    17521 tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt taccgtacag
    17581 ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct aaagatgtag
    17641 tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa
    17701 cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca gaagaaatct
    17761 ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct ggttatgaag
    17821 agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac ttccgtgata
    17881 atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa tctttggaag
    17941 caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag
    18001 atattgcaca attattgatc gtttcaccag actactttga agttatggca gcagatgaac
    18061 aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa aaagcagacg
    18121 gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat gaaaaacttc
    18181 ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg
    18241 aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg tcacaccccc
    18301 ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg ttgccagatc
    18361 cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat tgatccagct
    18421 ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac atccacgata
    18481 tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc attcaacgca
    18541 tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc actatttcta
    18601 aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc attcaaggat
    18661 aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg aagtgtcgta
    18721 tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg tttgaatacg
    18781 atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca
    18841 cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc agtctctgat
    18901 tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc caattcacct
    18961 tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata aatgcggata
    19021 gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc tactggcggt
    19081 tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg
    19141 ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc aaaagtaatt
    19201 tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt ggcaaaacgg
    19261 atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct gaaaatatct
    19321 tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg gtaatcagaa
    19381 ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat attttgtgat
    19441 ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata atagacacgg
    19501 ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa tgtctgataa
    19561 tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa ggtagttgct
    19621 tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg gtagtgctca
    19681 tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa atttcctttt
    19741 ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg
    19801 aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt tatattttat
    19861 cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta tcattttgga
    19921 tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg ttgatattca
    19981 ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt gcgatacgca
    20041 cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg acaacttcct
    20101 cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa tagttttcta
    20161 aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca tcatggactt
    20221 cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa
    20281 tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga taaggggaat
    20341 gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat gcagctgaga
    20401 catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca cctagttcta
    20461 aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa
    20521 aaaaccatca ttagtaagtg taacaaaa
//
LOCUS       DACDKL010000031        20038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDKL010000031 DACDKL010000000
VERSION     DACDKL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.031"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IV329_000900"
     CDS             complement(<1..450)
                     /locus_tag="IV329_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8940104.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
     gene            complement(555..1418)
                     /locus_tag="IV329_000901"
     CDS             complement(555..1418)
                     /locus_tag="IV329_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8940105.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1614..3713)
                     /locus_tag="IV329_000902"
     CDS             complement(1614..3713)
                     /locus_tag="IV329_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8940106.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3933..5042)
                     /gene="rpoD"
                     /locus_tag="IV329_000903"
     CDS             complement(3933..5042)
                     /gene="rpoD"
                     /locus_tag="IV329_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8940107.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5066..6907)
                     /locus_tag="IV329_000904"
     CDS             complement(5066..6907)
                     /locus_tag="IV329_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8940108.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7089..9482)
                     /locus_tag="IV329_000905"
     CDS             complement(7089..9482)
                     /locus_tag="IV329_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8940109.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9589..10449)
                     /locus_tag="IV329_000906"
     CDS             complement(9589..10449)
                     /locus_tag="IV329_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8940110.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10508..11029)
                     /locus_tag="IV329_000907"
     CDS             complement(10508..11029)
                     /locus_tag="IV329_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8940111.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11349..12068
                     /locus_tag="IV329_000908"
     CDS             11349..12068
                     /locus_tag="IV329_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8940112.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12094..14685
                     /gene="mprF"
                     /locus_tag="IV329_000909"
     CDS             12094..14685
                     /gene="mprF"
                     /locus_tag="IV329_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8940113.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14873..16060)
                     /gene="tuf"
                     /locus_tag="IV329_000910"
     CDS             complement(14873..16060)
                     /gene="tuf"
                     /locus_tag="IV329_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8940114.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16428..16784
                     /locus_tag="IV329_000911"
     CDS             16428..16784
                     /locus_tag="IV329_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8940115.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17025..18029)
                     /locus_tag="IV329_000912"
     CDS             complement(17025..18029)
                     /locus_tag="IV329_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8940116.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18195..19046
                     /locus_tag="IV329_000913"
     CDS             18195..19046
                     /locus_tag="IV329_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8940117.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19063..19308
                     /locus_tag="IV329_000914"
     CDS             19063..19308
                     /locus_tag="IV329_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940118.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19495..>20038
                     /locus_tag="IV329_000915"
     CDS             19495..>20038
                     /locus_tag="IV329_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940119.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVI"
BASE COUNT         5641 a         4116 c         3516 g         6765 t
ORIGIN      
        1 tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt ttactaaaaa
       61 atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt ctacttcctc
      121 gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc cttcttttct
      181 caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt tcaattctgt
      241 caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct tttttttcac
      301 taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa gcacaagcag
      361 agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct gttttgtttt
      421 tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt ataataatta
      481 caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa
      541 catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta
      601 atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca
      661 aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca
      721 gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt
      781 ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc
      841 tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa
      901 tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct
      961 ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct
     1021 aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct
     1081 tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta
     1141 tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca
     1201 aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt
     1261 tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg
     1321 cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata
     1381 ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa
     1441 aggttttctt aatacatttc tttgctatca taacataatg ttatctactt gaatagctga
     1501 gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt
     1561 tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg
     1621 ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa
     1681 gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata tagattataa
     1741 tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat
     1801 ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct
     1861 ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag
     1921 tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct
     1981 tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct
     2041 gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg
     2101 agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc
     2161 actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact
     2221 tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc agcatattga
     2281 tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg
     2341 attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg
     2401 atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata
     2461 tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca
     2521 ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca
     2581 tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct
     2641 tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca
     2701 ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc
     2761 gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc
     2821 acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct
     2881 atttttgctt caagatcagt accctctttg cttacagatt gattttcagc atcggattca
     2941 ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc
     3001 tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca
     3061 gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg
     3121 ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc
     3181 tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct
     3241 tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt
     3301 tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt
     3361 cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta
     3421 gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc
     3481 attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct
     3541 gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt
     3601 tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga
     3661 cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag
     3721 cgatcggctc ctttactagg tttacgctac catttaatct atcatattct atacgtcacg
     3781 ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa
     3841 aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac caaacagttt
     3901 tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt taattgtttt
     3961 gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg aatacgttcc
     4021 cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc atcatctaaa
     4081 ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag tacatcttcc
     4141 aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc ttcctggtct
     4201 tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc aagggaaaca
     4261 ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc catttccgca
     4321 ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg ttggatacgt
     4381 atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg tgcttggtca
     4441 gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga aaacttaaat
     4501 cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc
     4561 aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac tactaaacga
     4621 aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct
     4681 aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt cagatacata
     4741 cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt
     4801 tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt ttcatccacc
     4861 acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc tgcattcaaa
     4921 gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg tttgggtttg
     4981 ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt tccatttgcc
     5041 ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc gttaaattga
     5101 tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg gcttcttgct
     5161 gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc acatctagca
     5221 agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg atttccacca
     5281 ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat
     5341 aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca tcatgaataa
     5401 actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat gcacgatata
     5461 atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt gcagtttctt
     5521 gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc tgatttgtcc
     5581 ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac tctgttgaca
     5641 gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt tcaactagta
     5701 attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct tgttttaaat
     5761 aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct tggaaagatg
     5821 cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg actacgacag
     5881 aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt gcttcaaaac
     5941 ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc atttgttgga
     6001 tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata ccagcctgcc
     6061 atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt ttccggatat
     6121 cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt tctgtttctg
     6181 gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca agccagcgtc
     6241 ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact ctttgataaa
     6301 aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt
     6361 catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa
     6421 aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc aaatattcta
     6481 aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc gctgcttttt
     6541 catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct atagtacgct
     6601 gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc actgcttctg
     6661 gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct ttaccacaac
     6721 caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta cgttcttcat
     6781 gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca tattgtccaa
     6841 tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga ggaatccgtt
     6901 gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga agatatttca
     6961 ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa aggacacaat
     7021 tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca tttctatctc
     7081 attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc cttccagtac
     7141 aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac gctctctccc
     7201 aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata tatccctacg
     7261 atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc ttttacaact
     7321 gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc agcatttccc
     7381 catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac aagcaaggca
     7441 tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt tcttttatct
     7501 tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt
     7561 tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga taaaataaat
     7621 gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt gaccactcgg
     7681 cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa taccagattc
     7741 gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc tgcttctctt
     7801 aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc gctttctttc
     7861 aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac cgtatagcga
     7921 tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac atacgcatca
     7981 taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt gtctccagaa
     8041 atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg agcgtttttt
     8101 cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg tttttcttcg
     8161 ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt cgcttgggct
     8221 gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc tcctacgtac
     8281 acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga aaatgcgact
     8341 ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc ccgcatagtg
     8401 atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat ggctttctcg
     8461 tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt aatggtccct
     8521 gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata catgtcttga
     8581 acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat acttattagc
     8641 aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat aacagaaatt
     8701 ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc caaaggaata
     8761 atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc agaacgaata
     8821 gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc acctactcgg
     8881 ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca
     8941 gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc ttctgcccgt
     9001 ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt taccaaatct
     9061 cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat cactagtact
     9121 tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat ttcttgataa
     9181 attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga atacgcacct
     9241 acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt caatatatta
     9301 tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt ttgctggcca
     9361 gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt tttctccatt
     9421 ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac
     9481 aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa aagatgccaa
     9541 cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt acttatattg
     9601 ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa gaacaggaca
     9661 agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca taatcgttga
     9721 agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg agaaaatgta
     9781 atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga tcggcacaat
     9841 cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc ccatatacac
     9901 caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca atccgccggc
     9961 aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag cgccgtataa
    10021 taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag aagtatctct
    10081 tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac atccgacaaa
    10141 aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa aagaaagtag
    10201 aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat tttctttgaa
    10261 attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa agacagctgc
    10321 taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca ttcccttcac
    10381 aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca ttttttgcct
    10441 tttttccact atttgcgcac tccttctttc atttaatttt attcatgatt gaacatttcc
    10501 tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc attttgacaa
    10561 tatcaggata aaacatgtta cctccattat gatagagata gccgccattg taaagcaagg
    10621 actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt gggctcagca
    10681 catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga ttttttacat
    10741 aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt
    10801 gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca atactgtcag
    10861 ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg
    10921 tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca
    10981 tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg
    11041 aacgataagt tcttcctcat aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt
    11101 aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg
    11161 acatcttttt ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt
    11221 gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt
    11281 tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag
    11341 gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact
    11401 atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc
    11461 gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata
    11521 gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg
    11581 aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta
    11641 gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat
    11701 tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca
    11761 gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa
    11821 atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa
    11881 aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt
    11941 ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac
    12001 atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg
    12061 aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg
    12121 aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc
    12181 ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc
    12241 actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct
    12301 atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac
    12361 ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt
    12421 gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc
    12481 caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt
    12541 tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg
    12601 ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa
    12661 ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg
    12721 gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt
    12781 gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg
    12841 attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt
    12901 gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt
    12961 ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt
    13021 tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta
    13081 ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag
    13141 tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt
    13201 atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga
    13261 gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat
    13321 tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa
    13381 aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc
    13441 attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct gccggatttc
    13501 cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt
    13561 tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt
    13621 caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt
    13681 acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt
    13741 gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa
    13801 tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct
    13861 tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag
    13921 attgttatga ttttgcatga attcggctac gatttcatta agatggggga agaagcttat
    13981 gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg
    14041 aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag
    14101 caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg
    14161 ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa
    14221 aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa
    14281 gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat
    14341 ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta
    14401 gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt
    14461 gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat
    14521 aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg
    14581 attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa
    14641 ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat
    14701 agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta
    14761 caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac
    14821 cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt
    14881 cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg
    14941 ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag
    15001 gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga
    15061 agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg
    15121 tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa
    15181 gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat
    15241 ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt
    15301 tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac
    15361 gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg tcttccactg
    15421 gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat
    15481 ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg
    15541 aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa
    15601 cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga
    15661 aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag
    15721 gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg
    15781 taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt
    15841 tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg
    15901 gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg
    15961 tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt
    16021 taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat
    16081 aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa
    16141 atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc
    16201 aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt
    16261 tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg
    16321 aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat
    16381 agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa
    16441 ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt
    16501 tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc
    16561 aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag
    16621 atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg
    16681 cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg
    16741 cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat
    16801 tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata agaaggagtt
    16861 gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    16921 gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga cacaattcat
    16981 ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa acgattcaat
    17041 acttcatcca catttttgat catcacggat atcgtaccat ccggattatt cacaaattca
    17101 atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc gtttgatagc
    17161 ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt tacaggaggt
    17221 gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt tgccgtgatg
    17281 ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt ttcttctatc
    17341 gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt ctcaaatttt
    17401 tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa agaaggtgca
    17461 ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga aaactcatat
    17521 tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc ttcatctgct
    17581 ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc gatccctgct
    17641 tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa taaagataag
    17701 tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg tgcttcttca
    17761 ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc cacaaaattt
    17821 tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg tccagttttt
    17881 gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat atattctttt
    17941 gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc aataatatgc
    18001 agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt agtttacgga
    18061 aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat ttcaagagta
    18121 gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga acgaataaaa
    18181 aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat ggttacgaaa
    18241 tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct gtttcctctg
    18301 ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga tacaagaatg
    18361 aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag atattcttga
    18421 caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct tttgaagaat
    18481 cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg ccaaaccctg
    18541 ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca tttgaagaac
    18601 tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc caccatctag
    18661 atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc cttgcaccag
    18721 gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc ctcgaagcat
    18781 atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa atcgctgaag
    18841 cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac gaattccttc
    18901 ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta tttgattttg
    18961 aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc ggcaaagcaa
    19021 aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc aatcgaacgc
    19081 ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt ctttttgatc
    19141 gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca aatcatcgaa
    19201 gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga acatgaagtc
    19261 atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa agaatggaac
    19321 ggattatgta tatatacata atccgttcca ttttttattt ttctttcaaa aaacgggaaa
    19381 atacgcaaat ttttctgttg taatttgcgt atacgcaagt ttttttgcat tactcctttt
    19441 catttggctg tttttttctt ataatcgaaa gggtatgagc gaaggagaga acatatgaag
    19501 aaaaaacaga aacaaaaatt attaaatcaa ttccgtcctt cattggatgg tgtaagagga
    19561 caattattcc gttctctaga agaagagagc ttgcgacgtt atggtttgcc gttacaagtc
    19621 atgcttgatc ccaaaaaccg tcaaatctta acgatcggat tagctgggaa aacaaatgaa
    19681 gatacacgga tcaacgtgcc aatcgatgac aacttcaata ctgtattaaa gagaattcgt
    19741 tcaggagaaa acgggatttt cgaacgattc cgtgacaatc ttcttattga aatcgtgtct
    19801 tactggaatg atcaacgatt gaaaaacgat gaaccaactg cgcaagctgt tgcgacacca
    19861 gttacagaag aaacagtcag tgtgccagaa gaaaaaacgg aaaaaccaaa agcggacaca
    19921 actgaaaata cacgctcaac tgatacgaat gccgctttaa gttttgctga tttttcaaaa
    19981 gccgttgctg agtatcctaa attttacaca gaacaaaatg aacatgaagt agttatta
//
LOCUS       DACDKL010000032        19872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDKL010000032 DACDKL010000000
VERSION     DACDKL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.032"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IV329_000916"
     CDS             4..816
                     /locus_tag="IV329_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8940120.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IV329_000917"
     CDS             813..1613
                     /locus_tag="IV329_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8940121.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IV329_000918"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IV329_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8940122.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IV329_000919"
     CDS             3110..4819
                     /locus_tag="IV329_000919"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8940123.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IV329_000920"
     CDS             5031..9389
                     /locus_tag="IV329_000920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8940124.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IV329_000921"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IV329_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8940125.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IV329_000922"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IV329_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8940126.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IV329_000923"
     CDS             11227..11520
                     /locus_tag="IV329_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8940127.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IV329_000924"
     CDS             11517..11828
                     /locus_tag="IV329_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8940128.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IV329_000925"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IV329_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8940129.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IV329_000926"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IV329_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8940130.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14678..14851)
                     /locus_tag="IV329_000927"
     CDS             complement(14678..14851)
                     /locus_tag="IV329_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8940131.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15299..17661
                     /locus_tag="IV329_000928"
                     /pseudo
     CDS             15299..17661
                     /locus_tag="IV329_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18029..18952
                     /gene="truB"
                     /locus_tag="IV329_000929"
     CDS             18029..18952
                     /gene="truB"
                     /locus_tag="IV329_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8940132.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18956..>19872
                     /gene="ribF"
                     /locus_tag="IV329_000930"
     CDS             18956..>19872
                     /gene="ribF"
                     /locus_tag="IV329_000930"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8940133.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQAT"
BASE COUNT         6803 a         3504 c         4369 g         5196 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag cagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtggtc acagcagccg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcagtaata ggagtttacg agctgtttcg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtattg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattta atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg ttcgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcctgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccggataaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcgt attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct acaccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gattatcgga gacggtggcg caatgggcgg
     3721 gaaagactca aaagaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgcggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtactttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaat ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tcttcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaactg
     4501 gccaaaagga attgcgccat ttgatttgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaaac ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gtgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctgcatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggggttgcg ggattttttt gttttcatta
    14581 agacgtatta cctaagagtt ccatgacttt agtcttgcgg cgtgtcagct aaccaaaaaa
    14641 gagaaaaaga aaagtaaaaa attcgacttt cttttttcta gtagtatata aatttttgca
    14701 tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag
    14761 tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg
    14821 tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc
    14881 cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc
    14941 tgggatacca attcacatat tgacaaagat aacagagact tttacataaa acggatcaat
    15001 caggaaagga attgctcgat ccagccccag ttttccagca atgtgttaac tggcccagcg
    15061 tcattcagca aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata
    15121 acgacaagtt ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt
    15181 ttatacctgt tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag
    15241 gtttcaattg ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat
    15301 ggattattct agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta
    15361 tctttctgtt gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc
    15421 cgaagatgtc aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat
    15481 ttatgtccat ttgaaccgag tcatcaataa atatgatttg aacatgtttt atattgaagg
    15541 gccaggacac ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga
    15601 gatctatcca gaagtaacgg aagatgagac aggaatgcaa aaattattca aacgattttc
    15661 atttcctgga ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg
    15721 cggagagctt ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt
    15781 aatctctgct gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg
    15841 gttctcaaat gtctttatca atccagtgat agatggagct gttttaccga tccttcattt
    15901 aaatggtgca aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc
    15961 aaattacttt aacggacttg gctgggaacc tttctttatt gaaggaaatg attcagaaaa
    16021 attaaatcct gttatggctg aaaaaatgga tcaagcaatc gagaaaatta aatccatcca
    16081 aaaagaagct cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat
    16141 cgtacgcaca ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg
    16201 aaccttccga gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga
    16261 tgctttactc cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg
    16321 tattttagaa gaaatcagag aaatcgcgcc tactggtgac caaagaatgg caaaaaatcc
    16381 tatcaccaat ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac
    16441 tttgcaattt gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt
    16501 cgttcgcgag atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac
    16561 aaaatcaaac cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat
    16621 cgaaccagaa aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc
    16681 ggaacaccaa gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt
    16741 gcaagttacg aatcattctt gcgagtggtt gactctatgt tgactcagca cttcaaatgg
    16801 atgcggaaat cacatgacct ttcttggcga aatgattatc cttcgctaaa tctcattgca
    16861 agctcaacag tcttccagca agatcacaat ggatatagcc accaagatcc aggaatattg
    16921 acacatttgg cagaaaagaa ggcggagttc atccgtgaat atctgcctgc ggatgcaaat
    16981 acattgctcg cagtcatgga taaagcattc cgctcgtcag aaaaaatcaa tttgatcatc
    17041 tcttccaaac atccgcgtgc tcaattttac tcagcagaag aagcagccgt cttagtgaat
    17101 gaaggcttga aaatcattga ttgggcttcg acagcaaaag aggaggaacc tgaattggtc
    17161 attgctgctg caggaacgga atctaactta gaagcattag cagctgtaac tttgctgctg
    17221 gaagaattcc caaaactaaa aattcggttc atcaatgtcg tagatttatt gaaattacgt
    17281 catccaagtc aagatccgcg tgggttgagc gatgaagaat ttgatcaata ttttacaaaa
    17341 gacaaaccga tcttatttgc tttccacggt tatgaaacat tggtccgcac gattttcttt
    17401 gatcgtcata accatcattt aatgattcat ggctacaaag aaaatggaga tatcacgaca
    17461 ccatttgaca tgcgtgtagt aaatgaactt gatcgttatc atttggcaaa agacgcagct
    17521 ttaaaaatca aaggaagtca agcagaagat tttgcgaaaa aaatggatca gaaattacaa
    17581 gaacatcaaa actatattcg tgaaaatggt attgatctgc cagaagtatt ggattggaaa
    17641 tggaaaaatc ttgaccaata aaatgataaa atcgatcccg caaaatagaa ttttttacat
    17701 tgattaagaa actgaaaaaa taaataaaaa aaacggaaaa aagactggaa gaatggccag
    17761 tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc cacccataaa
    17821 tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta aaaaagcata
    17881 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
    17941 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
    18001 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttacctttat ggaaagaacg
    18061 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
    18121 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
    18181 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
    18241 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
    18301 agatatgtct ttatctgaaa aaaaaataga tgaagtgatg gcttcattta ttggtgagat
    18361 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
    18421 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
    18481 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
    18541 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
    18601 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaagga
    18661 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
    18721 gctgccaata gaaaaaggag tggaacggtt caaacgagta gatattactg atgaagtatg
    18781 gcagaaagta aaaaacggta tgagacttga ttatactgta tttgcgctcc cagaaatgcc
    18841 gtcagaagaa attgctttgt tttataaagg aaaagttgtc agtatttatc agccaaaccc
    18901 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
    18961 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
    19021 agtgctggga tttttcgacg gcgtgcatat cggacaccag aaagtcataa agacaggtat
    19081 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
    19141 tattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
    19201 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
    19261 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
    19321 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
    19381 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
    19441 aggtgaaaaa atcagctcta cccgtattcg tggattacta gacagcggac aagtaaaaga
    19501 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
    19561 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cggttcattt
    19621 gcctaaagaa ggtgtttatg tgtcggaaat aaaaattggg gaaacatggt atccagccat
    19681 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagagttgta
    19741 tattttggac tttaatcaag atatctatgg tgaacatgtc gcggtacgct ggaatcaatt
    19801 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaggagga
    19861 tgaacaagca ac
//
LOCUS       DACDKL010000033        19049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDKL010000033 DACDKL010000000
VERSION     DACDKL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.033"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IV329_000931"
     CDS             complement(<1..703)
                     /locus_tag="IV329_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940134.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IV329_000932"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IV329_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IV329_000933"
     CDS             1758..2837
                     /locus_tag="IV329_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8940135.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IV329_000934"
     CDS             2869..3462
                     /locus_tag="IV329_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940136.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IV329_000935"
     CDS             complement(3572..3799)
                     /locus_tag="IV329_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8940137.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IV329_000936"
     CDS             complement(3902..6490)
                     /locus_tag="IV329_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8940138.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IV329_000937"
     CDS             complement(6510..7169)
                     /locus_tag="IV329_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8940139.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IV329_000938"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IV329_000938"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8940140.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IV329_000939"
     CDS             complement(7942..8808)
                     /locus_tag="IV329_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8940141.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IV329_000940"
     CDS             8917..9558
                     /locus_tag="IV329_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8940142.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IV329_000941"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IV329_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8940143.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IV329_000942"
     CDS             complement(10999..11898)
                     /locus_tag="IV329_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8940144.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IV329_000943"
     CDS             complement(11900..12697)
                     /locus_tag="IV329_000943"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8940145.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IV329_000944"
     CDS             complement(12675..13379)
                     /locus_tag="IV329_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8940146.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IV329_000945"
     CDS             13510..14085
                     /locus_tag="IV329_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8940147.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IV329_000946"
     CDS             14203..14880
                     /locus_tag="IV329_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8940148.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IV329_000947"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IV329_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8940149.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IV329_000948"
     CDS             complement(16943..17941)
                     /locus_tag="IV329_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8940150.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IV329_000949"
     CDS             complement(18167..18820)
                     /locus_tag="IV329_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8940151.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5492 a         4125 c         3318 g         6114 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttct
//
LOCUS       DACDKL010000034        19013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDKL010000034 DACDKL010000000
VERSION     DACDKL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.034"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            20..574
                     /locus_tag="IV329_000950"
     CDS             20..574
                     /locus_tag="IV329_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8940152.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(747..1220)
                     /locus_tag="IV329_000951"
     CDS             complement(747..1220)
                     /locus_tag="IV329_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8940153.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1232..1768
                     /locus_tag="IV329_000952"
     CDS             1232..1768
                     /locus_tag="IV329_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8940154.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1808..2179
                     /locus_tag="IV329_000953"
     CDS             1808..2179
                     /locus_tag="IV329_000953"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8940155.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2463..3734
                     /locus_tag="IV329_000954"
     CDS             2463..3734
                     /locus_tag="IV329_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8940156.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3979..5004
                     /locus_tag="IV329_000955"
     CDS             3979..5004
                     /locus_tag="IV329_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8940157.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5298..6407
                     /locus_tag="IV329_000956"
     CDS             5298..6407
                     /locus_tag="IV329_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8940158.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6490..7386
                     /locus_tag="IV329_000957"
                     /pseudo
     CDS             6490..7386
                     /locus_tag="IV329_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7402..8226
                     /locus_tag="IV329_000958"
     CDS             7402..8226
                     /locus_tag="IV329_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8940159.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8258..9568
                     /locus_tag="IV329_000959"
     CDS             8258..9568
                     /locus_tag="IV329_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8940160.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9716..10975
                     /locus_tag="IV329_000960"
     CDS             9716..10975
                     /locus_tag="IV329_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8940161.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10985..11857
                     /locus_tag="IV329_000961"
     CDS             10985..11857
                     /locus_tag="IV329_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8940162.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11869..12699
                     /locus_tag="IV329_000962"
     CDS             11869..12699
                     /locus_tag="IV329_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8940163.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12739..14922
                     /locus_tag="IV329_000963"
     CDS             12739..14922
                     /locus_tag="IV329_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8940164.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14983..16203
                     /locus_tag="IV329_000964"
     CDS             14983..16203
                     /locus_tag="IV329_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8940165.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16200..17660
                     /gene="gtfA"
                     /locus_tag="IV329_000965"
     CDS             16200..17660
                     /gene="gtfA"
                     /locus_tag="IV329_000965"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8940166.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17737..18843
                     /locus_tag="IV329_000966"
                     /pseudo
     CDS             17737..18843
                     /locus_tag="IV329_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6452 a         2904 c         3710 g         5947 t
ORIGIN      
        1 tttaagaagg gaaacaatag tgagctataa tgtacaaccg ttaaggacaa atgaagagat
       61 acaagatttt cagttttggc taagaagaac aagtaatccg gaaagagata gttttttatt
      121 tttgtttgga atcaataatg ggttacgtat gtcagatatt ataggattaa aagttggaga
      181 tatacgaggc aaaagtaagc ctattattgt tgaacataaa acaggtaagc gaaaaccgat
      241 ttttatagat aatttgagag aagaaatttt attatatact gaaggtaaag aagaaaatga
      301 ttggttattt ccgagtcggc aacaaggtcg acatataaca agggatcgtg tctatcagat
      361 atatgcagat atagcagaaa aattaggacg agatgatata ggaacacata cattaagaaa
      421 aacttttggg tatcactact acaagaaaac tagagacatt gctaccttaa tgtttatttt
      481 taatcatagc tcgcaagcaa ttacaaaaag gtacattggt ataactgaag atgaaatagg
      541 agcatcactt cgtggattta aactaggtgt ataatttaaa aatttttata aacagtaaag
      601 acaaagatta aaagctattt ttatctagaa cgattttatt tcataaagtt cgcggaataa
      661 aagctggggt tactggtact tggtataggt tcgtgttata taaactaagt aattaaataa
      721 catttttagg ttggaaaagg atgaaattaa aaaaagagaa tcaatacggt ctcaaaaacg
      781 caaacgctgc ggaataagaa aaatactgat aactcaacgt ttagcagact atctaatagg
      841 gatagtctgc ttttttcgtt tttgaccata cttttgacct tctgcaaact tccttgcttt
      901 tcctctgtag aactcatgaa atctgcaaaa cgaattgccg tttcctctgt ctgctttttc
      961 gcgatatggg tgtatatatc atccatagtg gttttaatat ctgtatgtcc aagtcgaaca
     1021 tacacttctt tgacagacgc accagattca aagagcagac tacaatgggt atgacgaaaa
     1081 ccatgaagtg tgatatgttt taagttattc tctttaatga tgattttcaa attgtgattt
     1141 acatagtcta aataaagggg cttattttct tctgatgaaa acaaaagttg ttcaccatct
     1201 tttataggtt tgattctcat ttttagatat tatgcccgca cgtattcatc acatttctat
     1261 tgtgaatcgc tttattcgac cgacatttga cttttatcat aatatactcg gtttaaaatt
     1321 actaatgaaa acaattaatc aagacgatca taccatgtat cacctgtttt tttcagataa
     1381 tcaccaccgt gtgggaactg aactgacttt ttttgaagta caagaaggga acaaccagtt
     1441 ttttggaacc aatacgattg aacgaacgat actaaaagtt ccttccgaag catcattgca
     1501 tttttgggaa atatattttg aaacacaagg cgtttgccat tatggaatcg aatcattttc
     1561 tggacgacct atcttacgtt ttgaagggcc agatgcaaca cgattagctt tagttccctt
     1621 acgcgaattt gaagatattg atgattattt cccgtatgtc acagaccaga tccctacaga
     1681 gcatgccatt ttaggaattg atgccattca actacgcgta caatattccg atgcgactga
     1741 acgagagtta ttgtcggtag gctggtagca caaagaatac gtcagtttct tttcgggtca
     1801 tcaacgagtg acaatcttag aaaatcaaga cactcagttc tatcaagaag ttcagattat
     1861 tcaagatcgt gagaatccag ttgccattga agggattggt agcgtccatc atgttgcgtt
     1921 tggcgtagaa aataaatctg atttacaacg catcgacaaa caattacaag agcgcaattt
     1981 tataaattca ggaataaaag atcgtgaatt ttttgtatca ctttattatc gtgacccaaa
     2041 tcaattattg attgaaattg ctacagggga aggaaaccta gatgcaaaag cgtatgaaaa
     2101 tcaaagtcca cgctttgaag aaattccttt atttttaccg cagtacttaa atttcattcg
     2161 tgaacaagtg gagcaataat tagctaatca aatccatgac gaagagtaag aggttgactc
     2221 ataagtcaaa aaataaagaa aaacgcatca aatccattct aaagaatgtt gatttgatgc
     2281 ggttcctatt tttataaact atcattttcg actaataaaa ctacttatga gtcagcctct
     2341 ctttttttct tgatttttat acctctttct aggtaaacta aacatctgcc aaaaataccg
     2401 tgtctcattg aaacattctc aactatttca aaataaaggg gagtaccaaa tgaacgagta
     2461 tattgaaatt atccacgcaa gagaaacaac ttaaaaatgt cagtctcaaa attcccaaaa
     2521 ataaaattag tatttttacc agcgtatctg gttcaggaaa atcatcgatt gtttttgata
     2581 ctattaccca agaagctggt cgtcaactga atgacaccta tgatagtttt acccgtttat
     2641 ttttacctaa atacagtcgt ccagaggttg atgagattga taatctttct acggcgattg
     2701 tggtcaatca aaagaagatt ggtggcagtg cgcgctcaac gttagggacg attactaaca
     2761 tcaatgcgtt atttcgtgtg ttgttttcat gttttgcaac acctagttta ggatatgcaa
     2821 atgcttattc atttaatgat cctagtggaa tgtgttctgt ctgtcaggga atcggtaaga
     2881 cgattgcatt tttaatagga ttgctttatt tttcggctat tgataatgct gattttgtat
     2941 tttctgatcc atttaccgca gaagttttgt ctggaattga ggaacaggca aagaatcatg
     3001 gttattttgt attggtgcat aatgttaatt cagcagaaga tgttaaaact attcagagaa
     3061 attggcgttt tgaggggttc attgttgttg gtgtacgtgt aaaagaattt ttggaattaa
     3121 ataacgtttt tgatggtcct gttgtttata ttgatacgca tctttcaaaa gagacattaa
     3181 gagaacttga acaacaaatg aatcgtttat ttgtaaatac aaatgattac caagctagta
     3241 cattggcagt ggaacatttg attcattatg gtcatgagaa aatagcgttt ctttcttttg
     3301 actacattgt aggtgaacca agtgttattc aagaacgaca tcaagcttat atcgatacat
     3361 taaagaaata tcatttaacc tttaaatcgc attggatata taacaactct gaatttgagc
     3421 gaatttatca agaattagct gaatttactg caattgttgt tactggagat tatttagcgg
     3481 ctaaattagt aaaattttta aaaaataaga ataaacgagt gatggaacaa ttatcgatta
     3541 ttggatttga tgatattact tttgcagaat taatggatcc tgcgttgaca acaatacgac
     3601 taaatccagg taacaagggg gcgattgctt ttcaccaact gattggactg gccaaacaag
     3661 aagaaataaa aaatcaagta ctactattag agggagaatt gatttctcga gactctgttc
     3721 acaaaatcaa ataaaaaagc tctgtgaaat aagtatagtt aacaataaaa tatccgaacg
     3781 atagagagaa tgaactgtga tctagcagtt cctcgtcgca aattcactag aattagcatt
     3841 atttactcta aattgttcgg atattttttc ttgtttttaa ttatgattac agtgcctatt
     3901 tttaacgaaa tttgcttatt ttcaaaatgt atgattatat gttagtaaac actttcaaat
     3961 atgaaaagag gagctattat ggtaggaatt agggatgttg caaaacgggc gggtgtgtcc
     4021 attgcaacag tatcgcgcgt gctaaatgag gatccgacac tttcagtaac cgatgaaacc
     4081 aaacgaaaga ttgcagaatc ggttaatttt tatcattatg tcaaaaaaag tcccgttgca
     4141 aaaaatttaa caaatattgc attaataacg acagtttctg aagtagatga gttggaagat
     4201 ccctattttc gaacgattcg cagagggatt caagtcgaag cagaacaaag caaagtcaat
     4261 ttgaagaaaa ttattcgttt gtcagaaaca ccgttggagc aagacgattt gctaaaatgt
     4321 caaggtgtgt tgattattgg gcaggtgcta tcttctgtga ttgaagaagt atcagcaatt
     4381 aattcgaatg tcgtagtgat tgatgaccct agtattgaag ctgaagtaga tgcagtttat
     4441 actgatttaa caaaagcaac gaatcagcat ttagatcgat tgtataaaaa aggacaccga
     4501 aacattgcgt ttatcggagg aggacgtgtt cgtttagata gccaaggaaa taaacacgtg
     4561 tcagatgatg attatcgaaa aatagcctat gagacgtgga tgaaacaaaa aggattagaa
     4621 aaatatagtc aaatttattt agaaggctgg accacacttg acggaatgca ggcagcagat
     4681 ttacttttga aaaaacatgc taaagataag ttgccgactg ctatcatggt aggaaatgat
     4741 cctattgctg tgggggttta tcgttcactt caaaaaaatg gtctacatat tccagaagat
     4801 atttcgattg tgagttttga taatattgaa gttgctgaat ttttaacacc tgcattaaat
     4861 acggtgaatg tgaatacaga agaaattgga aaactagcag ttcgcatggt taatgaacga
     4921 ataaaagaag ttagaaatgt ttcgatcaaa gtgattgttt ctaataatat tattgttaga
     4981 gaaagtgaaa aaaatatcga ttaaaaaagt aaacatccaa aaaagggtgt ttactttttt
     5041 gtttactaat atttttagta aaaaatctcg acaaaacatt aagtaaacag ttatggtgtt
     5101 gttaacaaaa agaaaacgca ttcaaaacag gaggaattaa gatgaaacat ttcaaaaaaa
     5161 tagctttaac agggatagct gttttaactg ggacattact cgcagcatgt ggtgggggag
     5221 atagtaattc taaagacggg caagtaactt taagctatgc gagttgagat actgaccaag
     5281 ctccaggact aagaaaaatg ttagatgaat ttgaaaaaca aaatcctgat atcaaagtag
     5341 aaatggagac aacgccttgg gatcaatatt gggttaaatt agaagctgca acaacgggag
     5401 gtaatatgcc agatgtagta accatgcact ctagcgaatc ctataaatat atgtcacaga
     5461 atgctttgat gggacttgat gatgttatca aagaaaatga tattgacatg ggaaacttta
     5521 ctgaaggaat tgctgatttc tatacgcttg aagacgaact atacgcaatc cctaaagatg
     5581 cttctgtagt ggggctatgg tacaacaaag aattatttga taatgcaggt gtcgattatc
     5641 cagatgaaac atggacatgg gaaacactca gacaagctgc aattgattta acagatgaat
     5701 caaaaggcat ctatggtttt gcatcacata ttggaacaga gactggctac tggccattta
     5761 tttatcaaaa tgggggagaa gtatttgccg atgacaatac aaaatcaggt ttgaataccc
     5821 cagaagcaca agaagcttta gctttttatc gagatattgc acttgttgat catgcttcac
     5881 caacaattac agaattacaa gatcctgata agttaagtcg ttttcaatca ggtagattag
     5941 caatgatgat tgaaggaaac tggtttgcac cgggattttt gaaaaatgac tatctagttg
     6001 aaaatgttgc ttctgccgtg ttacctaagg gaaaagttaa tgcaacaatt acaaatggac
     6061 ttggttgggc tgccagtgcg agtacaaaat acccagaaga agtgaaaaaa ctaatggcat
     6121 ttttagctag tgaggacgcc aataaaatcc aagcggaaac gggggcatca atcccagcac
     6181 taaaaggctt tgaagacgag tgggctgctt cttcagatac aattgatttg agtgcttttg
     6241 gaaaaatgtt ggagtatgga gtccctcgtc catacaataa agatggatta cgagctgaac
     6301 aagcagaagt tcaaattatt aaccgtattc tgtctggtga agtaactgtt gaagatggaa
     6361 ccgctcaaat tgaaaaagaa gtcaatgcga tattgagcgg aaattaaatt agttaattat
     6421 acaatataca ggcgaaagga ttctatttcg tctgtatatt aatggatgaa taccaaaaag
     6481 ggggatatta tgaaaaatcg aatttttgga aaatatcgta aagatcaagt gatatggggc
     6541 tatattttta tttttccaac ggcattagga ttaattttat taaatatatg gccagcaatt
     6601 caaacagcaa ttttaagttt tcaaaaaacg gtagggttcg gtgatactga atgggtcgga
     6661 ttaagaaatt accaaaaaat gtttgcagac ggtgaagttt ttcagtcttt attaaacaca
     6721 ttaatttatg cagtggtttc gatacctagt attgttgtat tatccttgtt ggtggcagtt
     6781 ttaatgaata gaaaagttaa aggattgtcg atttatcgga caatttattt tctaccagtc
     6841 gttgctgcac catccgcagt cgcaatgatt tggcgctgga tgttcaataa tgattatgga
     6901 ctgattaata acatgttggc taaaataggt ctcgatggac cagcatggtt aacggatcca
     6961 tcgattgcga tttattctat tatcattgta ggagtttgga gtgctattgg ttataacatg
     7021 gtgttattac tagccggctt ataagaaatt cctaaagatt attatgaagc agcggaaatt
     7081 gatggtgcta gtccaatcca gcagtttttc aacattacgt taccattagt tacaccaaca
     7141 ttattttttg tagttgtaac aacagttatc aatgctttcc aagtatttga tgttattttt
     7201 atgatgatta ctcctaatag taccgccatg tttaaatcac aatcattagc ctatttattc
     7261 tataaacatt catttatttt aaatgacaag ggatatggtt cggcaatcgt tatgttcctt
     7321 ttagtgatta tattaattat tactgcaatt caaatgaagt tgcagaaaaa gtgggtaaac
     7381 tattaaggag gctatcaaat tatgaaaaat aaattttttt caaatgcgcc aatccatatc
     7441 atgcttattt taggtggtat tgcaatggca ttacctttta tttggatgat tttaacttca
     7501 gggaaaacgt tggttgaatc tacccaaatt ccacctacaa ttttccctga gaaatttcag
     7561 ctaagtaact atcaacaagt acttgattta ttaccattca tggcttttta tatcaataca
     7621 tttttaatgg ttgttggacg agtgattggc tcggttttat tttcagcaat ggctgcatat
     7681 gcttgtgcgc gtctaagttt tccagggaaa aatatccttt tcagcttagt acttattcaa
     7741 atgatgattc ccggttccat ttttattact ccacagtatt tattagcaca aaaattgggt
     7801 attttaaata caattccagc attaatatta ccaggggtag ttagtgcgtt tggtacgttt
     7861 ttattaagac aatttttctt aggtttacct gatgaattgg aggaagcggc ctatttagaa
     7921 ggtgccaata tttggacgat ctttttcaaa attatgttgc cgcttgctaa atcaggattg
     7981 gttgcacttg gaatattcac agcgttgttt gcatttaaag atttaatgtg gccattgatt
     8041 gtcaatactt caattgataa gatgccttta gctccgggat tagcaaactt acaaggacaa
     8101 tatgccacga acttcccaca attaatggca ggttcgttat tagcaatttg gccaatagtt
     8161 gtgatattca tcgttttcca aagaaaattc attgaaggaa ttgctacaac aggtagtaaa
     8221 ttataaaact attaattagt gattggagag taagtacatg acaaaaatta catttattgg
     8281 tgcaggaagt actatttttg caaaaaacgt attaggcgat gcgatgttaa ctccaagtct
     8341 acaagatgca catattgcat tgtacgatat tgatgaaaaa cgactaaaag aatcaaattt
     8401 aatgcttaaa aatattaata aaaattcgaa tgaaaatcgt gctgatattg tttcttatta
     8461 tgatcgcaaa gaagcgttaa gagaggctga ttttgttatc aatgctattc aagtgggtgg
     8521 ttataaacct tcaacagtga ttgattttga tattcctaaa aaatatgggt tgcgacaaac
     8581 tattggtgac accatgggga ttggtgggat ttttagaacc ctacggacac taccagtaat
     8641 gttagacttt gctaaagaaa tggaagaagt ttgtccaaat gcttggttat tgaactatac
     8701 aaatccgatg gcgatgttaa ccatgggtgt tttaaaagcg acgagtatta agactgttgg
     8761 tttatgtcat agcgttcaag tatgcgtacc agaattgttt gagcatttag gaatagccga
     8821 tcaatatgac ttaaatgact ttcaatggaa aattgcaggc attaatcata tggcattctt
     8881 gttggaaatt acaaaaaatg gggaagattt ctatccaaca attcgtcagt tagctgcaga
     8941 aaaaccaaat ccacatccag attctgtacg ttttgaattg ttgaaacgtt ttggttacta
     9001 tattacagaa tcaagtgaac acaatgcaga ataccatcca tactttatta aatcaaaata
     9061 cccagaattg attgacgaat tagggattcc gttggatgaa tacttacgtc gttgtgaaca
     9121 acaaattaat gattgggaaa gcatgcgaga ggacattgta aataatgggg agttaacaca
     9181 tacgagaagc cgtgaatatg cttcatatat catggattct attacgacag gaactccgtc
     9241 agtgattgca ggaaatgtct taaataaagg cttaatcaca aacttaccga cagatgtttg
     9301 tgtggaagta ccttgtttag tcgataaaaa tggcgtgcaa ccaacctatg tcggtgaatt
     9361 gccaacacaa ttagctgctt taaatcggac gaatatcaat gttcaagaac tgacagtaga
     9421 agccgcttta acactagata aatcaaaaat ttatcaagca gcgtatttag atccacatct
     9481 atcatctgaa ttgtctttaa cagatatcaa aaatttagtg gatgatttaa tcgaagcaca
     9541 tggggactat ttacctgaat ataattaaaa agaagctgaa agagtaagga attttcaatt
     9601 ccttactctt ttcttagtta aatttagtaa aaatattgac aaaacactta gtaaacagtt
     9661 acattagtgt taacaaaaag aaaacgcatt caaattgtat aaggaggagc ataaaatgaa
     9721 gtggtctaaa aaaattattt tagcttgtat agcactagga gcaacgatct ctttgactgc
     9781 ttgtggaaaa aatcaggaag atgcaagcgg taaggtagaa atagagtttt ttaatcaaaa
     9841 aaaagaaatg actcaaacga ttcaggagat tgctaaagat tttgaagcaa aaaaccctga
     9901 tattcatgta aaagttgtgg atgtaccgaa tgctggtgaa gtaattaaaa cacgtatgct
     9961 tgcaggcgat gtaccagatg tcattaatct ttatcctcaa agtatagaat tgaaagaatg
    10021 ggcgaaagct ggttatttag aggacttaac cgaagaaccg tatttagaga atatcaaaaa
    10081 tggatatgca caacgttttg ctattgagga tcgtgtatat agcataccat taaccgctaa
    10141 tgtctatggt ttttactata acaaaactgc ttttgaggaa atggggattc aagcaccaga
    10201 aacttgggca gaatttgaaa aaatagtagc tgagattaaa gatcaaaaca aagtaccgtt
    10261 tgcgattgca ggagcagaag gctggacttt aaatggttat caccaactag ctatagcgac
    10321 tgctgctggt ggtgaagagg aagcgaacaa tgtttggcgt ttctcagaag ttaatggaat
    10381 taatgctgaa agcaaagaaa tgcaaaaaga ttttaatcga ttagatttgt taagagaacc
    10441 aaaagctttg caaaacaact ggcaaggtgc cggttataac gatacagtag ttacttttac
    10501 aaaaggtgat gcgttgatta tgccgaatgg ttcatgggca atgccgatga ttcattcaca
    10561 aaatccagat tttgaagtag gaacttttcc ttttccagca gatgaagcag gtcaaagtct
    10621 aacgattggc gcaggagacc tagccttatc agtttctgca acttctcaac ataaagaaga
    10681 agccaaacgt tttgtagaat atatgacaac acctgaagcg atgcagaaat attatgatgt
    10741 agatggtgca ccgtgtgctg ttgagggcgt tatcgagaat actcaggatt caccattaag
    10801 tggtttaact gaacttgctt ttactgatcg tcatttagtt tggttagcca aagattggaa
    10861 tagtgaaaat gatttttata ccttaacaac aaattattta cacaatggca atcaaaaagc
    10921 catgattaac gcattaaatg ctttctttaa tccaatgaaa gctgacggaa ggtaggaaaa
    10981 taaaatgaaa ataaaagaaa ttttaaatcg ttactgggca atcatttttg tagcaattcc
    11041 aattattttg cagatgattt ttttctactt tccaatggtt caaggtgctt tttacagttt
    11101 aactaattgg actggtctga cttataattt tgattttgtg ggtttgaaca attataagat
    11161 tcttttttct gatccgaagt ttatcaaatc gattggcttc actttaattg tgacgctttg
    11221 catgattgct ggacaaattg gttttggtat tttgattgcg cgggcgttga acgcaaatat
    11281 taaagggaaa acattcttta gagcatggtt cttttttcca gcggtcttat cgggcgtggt
    11341 tgtttcttta gtatttaaac aaatttttaa ctatggttta ccaaaaattg gtagtatgtt
    11401 aggcattgaa tttttacaaa ccagtctttt aggaacaaca tggggagcga tttttgcagt
    11461 aatttttgtt ttactttggc aaggaattgc catgccagtc atcattttct tagcgggttt
    11521 tcaaagtatt ccacaagaaa ttattgaagc tgctagaatg gatggggcga atgataagca
    11581 aattttttgg aatattgaaa tcacctactt attaccaagt atttcaatgg tatttattat
    11641 ggcattaaaa ggtggcttaa cagcatttga tcaaattttt gctttaactg gtggtggacc
    11701 aagcgatgcc acaacatctt tggggttact tgtttataat tatgctttta aaaacaatca
    11761 gtttggttat gcgaatgcta ttgctttagt cttatttatc ttaattggga ttgtttcact
    11821 ggcacaatta aaattatcga aaaaatttga aatctaggag gtttcccaat gaaaacatca
    11881 aaagaaaaaa atatttctaa atacttgctt ttattattag ggggcttcat gattttagtt
    11941 cccttagttt caacgctatt cggttcattt aaaacaacac ccgatattat gaataatttc
    12001 tttcgttttc ctaatccagc gactttggaa aatttccgaa gattgttagc ggatggaatt
    12061 ggtcattact tttggaattc ggcaatcatc acaatcattg cagtagttgc tgtgacagca
    12121 attattccta tggctgctta ttcactagca agaaaaatga ctcgtgggaa agcatacgga
    12181 ttgatgtatt tatttttaat tttaggaatt tttgttcctt tccaagtaat tatgattccg
    12241 attaccgtta tgatgagtaa aattggttta gcaaatatgt ggggattaat cttactctat
    12301 ctcacttatg ccgtaccaca gacactattt ttatatgttg gttttattaa actaaatgtg
    12361 cctatcagtc tggatgaggc tgctgaaatt gatggggcta gtcactttac aacttatttc
    12421 aaaattgttt tcccaatgtt aaaaccaatg catgcgacga ctattattct taatgctcta
    12481 tggttttgga atgatttcat gctaccatta ttaattttaa atcgtgattc taatatgtgg
    12541 acactaccat tgtttcaata taattatacg ggacaatatt ttaacgacta tggtccaagt
    12601 tttgcttcct atacagtagg aattgtcacg attacactag tttatcttgc tttccaaaga
    12661 agtattatag ctggaatgag caatggagca gtgaaataac caataaatta ttctcattat
    12721 aaaggagttc ataaacttat gattatattt gacaatcaca cacaaacatt tcatttacaa
    12781 aataaaaaaa ttagctatct attaggaatc gaagaaaata attatttaac gcatctttat
    12841 tttggaaaaa agattacgca ctattctggt gggtatcgct acccccgaac agatcgttcc
    12901 ttctcaccca acaatgcaaa tgcgcaagat cgtttattat ctttagacac attaccatta
    12961 gaatttccag gatatggaca tggtgatttt agagaaccgg cgcataatat taaattagga
    13021 aacggtagtc gtgtaaatga ttttcgttat gatagctacg aaattcaaaa aggaaagccc
    13081 agcttagcgg gattgccagc cttatatacg gaagatgatg atgaagctga aacgctgatt
    13141 attcaattga aagacgcgat tagtggattg actttacagt tgttctacac aatttatcaa
    13201 aatcacagtg taattattcg ttctacgcaa ttagtaaata atagtgatga aacagtggag
    13261 attaaccgct tagctagtca atcaattgat tttccaaatc gtccactcga gttaattcat
    13321 ttaaatggta cctgggcaag agaacgtcaa atgactagag aaaaaattca tacagggata
    13381 aaagtattag atagtaaacg cggatcaagc agccatcaac aaaatccgtt tgttgcttta
    13441 gtagatgcca aaacagatga atttcaagga gaggcatatg gatttaattt agtttatagc
    13501 ggtaatcacg aaacggtgat tcaaaaagat ccatttgacc aaacacgggt cattatggga
    13561 attaattcat ttaattttgg ttggcaacta aaaactggcg actcatttca aacgccagaa
    13621 gtcgtgatgg tttatagtaa tgaaggtctg aatacgttat cacagacgta tcatgaattg
    13681 tataatcacc gtttaattcg tggaaactat cgtttaaaag aacgtcccac gttaatcaac
    13741 aactgggaag caacgtattt tgattttaac gaagaaaaaa tcatgaatat tgtcgatgat
    13801 gcgaagaact tgggcattga aatgtttgtt ttagatgatg gttggtttgg cggtcgtgaa
    13861 aatgacttca cttcattagg tgattgggtt gaaacagaag gcaaacttgc ttgtggtttg
    13921 gaaaatttag ccaagcaagt gcatgataac gggatgaaat ttgggatttg gtttgaaccg
    13981 gaaatgattt ctgaagatag tgaactattt cgagcacatc cagattgggc attgggtatt
    14041 ccaggtcgtg accgctcaat tagtcgaaat caatatgttc ttgatttttc aagacaagat
    14101 gttcgtgata atatctatca acaaatgacg gcgatttttg atcgtgtacc gattgattat
    14161 gtgaagtggg atatgaatcg aaatatgaca gaagtctatt ctgtcttgtt ggactcaacg
    14221 atgcaaggtg aagtggctca tcgttatatg ttagggttat atgaattttt agaaaagtta
    14281 acaacgaatt atccaactat tttatttgaa agttgttcgg gtggaggcgg tcgttttgat
    14341 gcaggtttct tgtattacat gccacaaact tggacgagcg acaataccga tgccgtggct
    14401 cgtctgaaaa ttcaatatgg aaccagctta gtgtatccaa tttcaagtat gggtgcgcat
    14461 gtttcagcag tcccaaatca tcaaacagga agagaaacga gtttaactat tcgtggcaat
    14521 gctgcgatgg ctggtgtttt tggttatgag ctagatctaa cgcaattaac ttctgaagaa
    14581 aaagcagaaa ttaaagaaca agttgccttt tataaaaagc atcgccaact tttgcaatat
    14641 ggaacttttt atcgcttgga aagtccgttt gaaggcaatg atactgcatg gatgtttgtc
    14701 tcaaaagacc aaacagaagc catggtcttt tatttccgag tattagcaga agctgcgcat
    14761 ccattagtca cattgaaatt agcaggatta gcagaagaag caacttatca aatggaagat
    14821 gtaacatttg ctggcgatga attgatgaat ctcggttttt atattgactc agaactacat
    14881 ggtgattatg cgacacaacg cttttatttg aaacagatat agtagcttga atttacaata
    14941 taagtgcaac accaaaagag actcattcca aacgaagaaa atatgtttta tttacacgca
    15001 tttactaaaa agcagcctga tttgaattgg gaaaatgaag aagtacgtga agaaatctat
    15061 gcaatgatta actattggtt agataaagga ctaggcggat ttagaatcga tgcgatatta
    15121 aacatcaaaa aaaaactaga gtatggcatc tttgaatctg atggagaaga tgggttagcc
    15181 ttcattgggc cctggattct taatcagtcg ggcattgaag tgtggttaaa agaaatgaga
    15241 gagcggacat ttaaaccaca taatagtatg acggtagcag aagccgatgt tcctaatgaa
    15301 cgtttagatg aatatattgg tgaagatggc tattattcga tggtttttga ttttagttat
    15361 acggatattg atgtacctga aactggtgaa tggtttaaag atagccaatg gacatggacg
    15421 gatatgcgta ccaatatttt tacgaatcaa ttagttacgc aagataaagg ttggggcgcc
    15481 ttatatttag aaaaccacga tcaaccacgt tcaattaata aatatattcc agaagaatgg
    15541 atcaatgatt atagtaaaaa aatgttggcg acgcttttta tgttactaag aggtacccct
    15601 tttatttatc aaggacaaga actaggaatg accaacatag aaatggcatc attagaagat
    15661 tttgatgatg tagctacgca ttctcagtat aaacgggcat tagaatatgg attggcacca
    15721 gagcaagcat tgaaagctgt caataaacga agtcgggata attctcgcac acctatgcaa
    15781 tggaatacgc aaaaaaatgc cagattctca acttctgata atgtatggtt aaaagtaaat
    15841 cctaattatc cagaactcaa tgcagaagct cagttaacca atcaacagtc ggtattcaat
    15901 tattatcatc aattaattaa attaagaaaa aatagtgtat acaaagacgt gctaatctac
    15961 ggacaatttc taccagtgaa agataaaaat gaacatattt tgctttatga acgccaatta
    16021 gaagataaaa tcgtattagt tatcttaaac gttacagcaa cagaacaaga atattatgta
    16081 gatgaacagt accaacaagt attaatcaat aattatgaag atgttttatt agatcaaagt
    16141 cgaaaattgc gtttacgacc gtttgaaagt attgtattag ctaattatgg aggacaatta
    16201 tgaaaattaa aaacgaagca atgctcatca cctatgcaga tagtttagga aaaaacatgg
    16261 gcgaattaaa acaagtctta gacaaacatt tacaaggtgt tattggtggt gtacatctgt
    16321 tgcctttttt tccatcgaca ggtgatcgag gctttgcgcc gagtgattat acgacagttg
    16381 atccagctct tggaacatgg gaagatgtgg aagcattagg tgaagattat tatttgatgt
    16441 ttgattttat gattaatcac atttctcgtg aatcaaaatt tttccaagat ttcaaaaaaa
    16501 accatgagca gtcaccatac aaagaaatgt ttatccgtat tcatgaattt ttcccagaaa
    16561 atcgaccaac acaagaagat attgatttaa tctacaagcg taaagacaaa gcgccgtttc
    16621 aaacggtaga atttgcggat ggtacgacag aagaggtttg gaataccttt ggggaagaac
    16681 aaattgattt agatgtgacc aaagaagttg tgaaagaatt tattcgagaa accatcaaag
    16741 atatggcgaa ccatggctgt tctttaattc gattagatgc gtttgcttat gcgattaaaa
    16801 aattagatac caatgatttc ttcgtggaac cagatatttg ggaactttta gacgaagtgc
    16861 gtcaagaagc agcgaaatat gacgtcgaat tattaccaga aatccatgag cattattcca
    16921 ttcaaatgaa aattgccaat catgattatt atatctatga cttcgccttg ccaatggtca
    16981 ctctgtattc actttatagt ggaaaatcag aacgtttagc caattggttg aaaatgagtc
    17041 cgatgaagca gtttacaaca ttggatacgc atgatggaat tggtgttgtg gacgctcgtg
    17101 atttattaac agatgaagaa ttggattaca cctctgagga attgtataaa gtcggggcga
    17161 atgtgaagaa ggtctattca agtgccaatt acaataattt agatatttat caaattaaca
    17221 gcacctatta tagtgcatta ggcgataatg accgtagcta tctgttagca cgtgcgattc
    17281 aagtatttgc gccagggatt ccacaaattt attacgttgg gttattagca ggaaaaaatg
    17341 atattgaact gttggaagca acgaaagaag ggcgtaatat taatcgtcat tactatgatt
    17401 tagccgaaat tgaagaagaa gtgcaacggc cagtgattca acaattattt gcacttttaa
    17461 aatttagaaa tgaatctgcg gcgtttgatt tggatggaac gattgaagta gcaacgccat
    17521 cagaatcaga aatagtgatt acacgtaaaa ataaaaacgg tagtgatagt gcaacattaa
    17581 cggctaactt ggatacgaaa gaatttatca ttgagtctaa agatataaaa attttcacta
    17641 gtgttgcatt aaaattttaa cctattccat agaacaaaaa atcctttata ctagcacttg
    17701 gatgacttat ccaagaccaa taaaaaggac tcatacatgg ataagtataa aacaaataca
    17761 gcatttaata aatggttttc tgccataaaa ttaggaaaac tttctgtcaa ctcccaaaaa
    17821 tgtatttttg attacaattc gtatcgtaaa aaactgtctt ttgaagctgt gctcaagctc
    17881 tttctctacg cagttaatga agaaaaagaa agtttgcgtg atttgagtac agctcttatc
    17941 gaccgatcgc tccaactaga aaccgatgtg tcatcgatta gtcataccca attgtctcga
    18001 gcatttaatg cacttgaacc taaagtgtta gaagaaattt ttcaacaact gttagaaaaa
    18061 gttcaacatc aaatcaaacc aaccaagcga aatcatctgt atctgattga ttccagtacg
    18121 ttttcgctta gtttaaaacg tcataagtgg gcgactttcc gtcaaacaaa atcaggtgtc
    18181 aagttacact taaactattg ttatatggac gatactacga tgtatccaac tgattttacc
    18241 ttaacaaatg cccatgaaca tgatctcaat caactaccag tattggtgaa tcaaccagag
    18301 gcaacttacg tgtttgatcg tgggtattag gactttgaaa agatggatca gatgcattgg
    18361 gatggctatt tcttcgtcac tcggattaaa aaaaacacca aagttcacgt gatagatact
    18421 ctggaaacat cgccagaaac agaaattcta cgagacgagc tggttcgttt aggatctaaa
    18481 acctatctaa ccgccaattt ccgactagtc actgttcaag ataagaacgg acgtgtgttt
    18541 caatttatca ccaatcgaat ggacgtctct tcaaaagaaa tctctgatat gtatcacgct
    18601 cgttggcaaa tcgaattgtt tttcaagcac atcaaacaac acatgaccat taaaaccttc
    18661 ttttctcaaa gtgaaaaagg cgtgcagaat caattgattt taacgatgat ttccgctctc
    18721 ctcacgtttc tcatcaaatt ggagacgaaa acggaaaaat cagtgttcca aatcaaacga
    18781 ttctttcgtt atttactgtt tcagcccttt gaatgttgtc tagaaaaatt aattcccaca
    18841 tgagaaaaaa gaaatttggc tcgttacttt tggttttcag ttgaataaca gataaaaaat
    18901 tttctggaaa aagtcatcct ctgcgtcagc acagtttttt ttgacttatt ttcagaggaa
    18961 atagttttca gaatattcta aaatgtttta tgcaacacta gtgatatttt tta
//
LOCUS       DACDKL010000035        18928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDKL010000035 DACDKL010000000
VERSION     DACDKL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.035"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            592..1920
                     /locus_tag="IV329_000967"
     CDS             592..1920
                     /locus_tag="IV329_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8940167.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IV329_000968"
     CDS             2122..2955
                     /locus_tag="IV329_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8940168.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IV329_000969"
     CDS             2971..3708
                     /locus_tag="IV329_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8940169.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IV329_000970"
     CDS             complement(3863..4831)
                     /locus_tag="IV329_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8940170.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IV329_000971"
     CDS             5063..5896
                     /locus_tag="IV329_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8940171.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IV329_000972"
     CDS             5930..6769
                     /locus_tag="IV329_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8940172.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IV329_000973"
     CDS             6795..7355
                     /locus_tag="IV329_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8940173.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IV329_000974"
     CDS             7554..9275
                     /locus_tag="IV329_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8940174.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IV329_000975"
     CDS             9440..10582
                     /locus_tag="IV329_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8940175.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IV329_000976"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IV329_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8940176.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12382..13029
                     /locus_tag="IV329_000977"
     CDS             12382..13029
                     /locus_tag="IV329_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8940177.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13422..16067
                     /locus_tag="IV329_000978"
     CDS             13422..16067
                     /locus_tag="IV329_000978"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8940178.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16377..17690
                     /locus_tag="IV329_000979"
     CDS             16377..17690
                     /locus_tag="IV329_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8940179.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17680..18333
                     /locus_tag="IV329_000980"
     CDS             17680..18333
                     /locus_tag="IV329_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8940180.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDLEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18388..>18928
                     /gene="radC"
                     /locus_tag="IV329_000981"
     CDS             18388..>18928
                     /gene="radC"
                     /locus_tag="IV329_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP8940181.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPS"
BASE COUNT         6368 a         3201 c         4002 g         5357 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaaaaagc
    12181 tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttacgtttg
    12241 gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc tcacaccctt
    12301 ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa atatgtgaaa
    12361 ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa agcaactgcg
    12421 aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg tataagtaga
    12481 atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat ccgcagagac
    12541 ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc ttttcttatc
    12601 gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt agtaggatat
    12661 ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa tctaaatatc
    12721 gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg agaaatgatc
    12781 tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt agcaatctct
    12841 acggtaccaa gtaaatattc acaatctgct attgatgaaa ttgtcaaagg aaatgtaacc
    12901 gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa tgttcaatac
    12961 attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta ctcagaagtt
    13021 ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg taattctttc
    13081 aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga ctaaaaaaaa
    13141 aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac attcattctt
    13201 agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta gcttgggagt
    13261 ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact tgagaggcaa
    13321 taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct agaatacgaa
    13381 agtattttag gacgtttttt agtttaaagg aggaaaacag catgacggaa gaaaataacc
    13441 tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat tggttagaac
    13501 aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc gtaatcccgc
    13561 ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc ttgcaagata
    13621 tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct ggtatggacc
    13681 atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa gggatttctc
    13741 gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa gaagagtatg
    13801 cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac agtagggaac
    13861 gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt accttatacg
    13921 aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa gcacgaacag
    13981 ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat catatgagct
    14041 atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca gaaacgatgt
    14101 tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg ctcatcggca
    14161 aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat gaatatgtag
    14221 atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca aacgactttg
    14281 aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat ggtacgatga
    14341 atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa gcaatcgtgc
    14401 aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac agtgtgggtc
    14461 attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg tttgtaaaaa
    14521 tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca gtggaattct
    14581 tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat gattgggtaa
    14641 tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa gaaacaggtg
    14701 aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa gatcctgatg
    14761 ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc tggccagata
    14821 cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc ggttatgaca
    14881 tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact ggcgaacgtc
    14941 catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg aagatgagta
    15001 aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga gcagatgctc
    15061 tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc agctatgaaa
    15121 aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt ttcgttatca
    15181 tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa tcagtggctg
    15241 accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag ctatttgatc
    15301 gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat gatttttgtg
    15361 actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt tcaaaacaaa
    15421 ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg catccaatca
    15481 tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat tctctagtag
    15541 tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct aaagggatgg
    15601 aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg aatacaccat
    15661 tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat caatttttag
    15721 ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg atatcaagtg
    15781 agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa atcttcttgc
    15841 cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa gaactggaaa
    15901 agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt tttgtttcta
    15961 acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat ttagagaagc
    16021 aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa tagaaaaaga
    16081 gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt tacaggaaag
    16141 agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat tttgtcgcat
    16201 ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca tgtatcaaga
    16261 gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt tgtcatactt
    16321 ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa ttgttgatga
    16381 cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca cgtcctggcc
    16441 ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa gtaaaaacga
    16501 tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga agcctattag
    16561 aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg ttcaatgaac
    16621 ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt gtaaaaaaat
    16681 atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt actgaattcg
    16741 agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac gtagcgatta
    16801 tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca atgctgacgg
    16861 gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt gaagaaatcg
    16921 ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga aatattgccc
    16981 cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg ctttatgtat
    17041 acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga gaagtatttg
    17101 aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta gggcaacatc
    17161 aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa ttggaaaagc
    17221 tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg ccaggaagaa
    17281 tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat acgcatgcaa
    17341 tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc aacattttgt
    17401 tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag caagtaccaa
    17461 atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta agtgaagtat
    17521 cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga ttagcagata
    17581 tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt tatttcattt
    17641 cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa tgcagtgatt
    17701 tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc cacacaagta
    17761 gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt cttaggtgtt
    17821 tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg caaaacaagt
    17881 gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc gcaaggagcg
    17941 gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca tcagattccc
    18001 aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa gaagaacaat
    18061 ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc aaaaccagac
    18121 ctagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga aacattgatt
    18181 ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc ggtagtcatg
    18241 gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg ggttgtttct
    18301 tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg tatcttattt
    18361 tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga agtacctact
    18421 agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc ggatcaggag
    18481 cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc gattgctgga
    18541 aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt gcatgagtta
    18601 gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt gatcgaatta
    18661 ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac cagctacgaa
    18721 ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt ggtttgtatt
    18781 tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg ttcgctgaat
    18841 cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg tgcagcaagg
    18901 atcgttttag cgcataatca tccttctg
//
LOCUS       DACDKL010000036        18166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDKL010000036 DACDKL010000000
VERSION     DACDKL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.036"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(111..566)
                     /locus_tag="IV329_000982"
     CDS             complement(111..566)
                     /locus_tag="IV329_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940182.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="IV329_000983"
     CDS             complement(720..1928)
                     /locus_tag="IV329_000983"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8940183.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="IV329_000984"
     CDS             complement(2040..2372)
                     /locus_tag="IV329_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8940184.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="IV329_000985"
     CDS             2520..3395
                     /locus_tag="IV329_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8940185.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="IV329_000986"
     CDS             complement(3477..4058)
                     /locus_tag="IV329_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8940186.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="IV329_000987"
     CDS             complement(4064..5323)
                     /locus_tag="IV329_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8940187.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="IV329_000988"
     CDS             complement(5325..6371)
                     /locus_tag="IV329_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940188.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="IV329_000989"
     CDS             complement(6606..6881)
                     /locus_tag="IV329_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8940189.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /locus_tag="IV329_000990"
     CDS             complement(7124..8434)
                     /locus_tag="IV329_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8940190.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IV329_000991"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IV329_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8940191.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="IV329_000992"
     CDS             complement(9980..10660)
                     /locus_tag="IV329_000992"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8940192.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="IV329_000993"
     CDS             complement(10709..11344)
                     /locus_tag="IV329_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8940193.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="IV329_000994"
     CDS             complement(11410..12813)
                     /locus_tag="IV329_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8940194.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="IV329_000995"
     CDS             complement(12800..13831)
                     /locus_tag="IV329_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940195.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="IV329_000996"
     CDS             13862..14083
                     /locus_tag="IV329_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8940196.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="IV329_000997"
     CDS             complement(14248..14985)
                     /locus_tag="IV329_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8940197.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="IV329_000998"
     CDS             complement(15138..16478)
                     /locus_tag="IV329_000998"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8940198.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..>18166)
                     /locus_tag="IV329_000999"
     CDS             complement(16715..>18166)
                     /locus_tag="IV329_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8940199.1"
                     /translation="IAVLTWFIPAGHYSVDDAGNIVAGSYERVQQNPQGLWDIFMAPI
                     NGMLGVPAGELTKETPAAIPISFFILVVGGFLGIINKTGALDAGIASVVKKFKGKEKM
                     LIPVLMLLFALGGSTYGMAEETIPFYALLIPVMMAVGFDTVVAVAIVLVGSQVGCLAS
                     TVNPFATGVASQAVGISMGEGIGLRFLMFVILLAISIVYVYRYASKIEKDPTKSLVYN
                     QRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFITFGIMIISLIPWTTLNANFTIFESL
                     TNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFLVMSIVVAITYGMKESEFVSEFLAG
                     ASDLIGVAIVVAVARGIQVVMNNGLITDTILHWGEQGLSSLSSSVFIIITFIFYIPMS
                     FLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITAYQSASGLVNLITPTSAIVMGAVAI
                     ARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5120 a         3880 c         3081 g         6085 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgat
//
LOCUS       DACDKL010000037        17896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDKL010000037 DACDKL010000000
VERSION     DACDKL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.037"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..126
                     /locus_tag="IV329_001000"
     CDS             <1..126
                     /locus_tag="IV329_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940200.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            113..1285
                     /locus_tag="IV329_001001"
     CDS             113..1285
                     /locus_tag="IV329_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940201.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(1384..2349)
                     /locus_tag="IV329_001002"
     CDS             complement(1384..2349)
                     /locus_tag="IV329_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8940202.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     regulatory      2887..2933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2946..3449
                     /locus_tag="IV329_001003"
     CDS             2946..3449
                     /locus_tag="IV329_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8940203.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     gene            complement(3505..4149)
                     /locus_tag="IV329_001004"
     CDS             complement(3505..4149)
                     /locus_tag="IV329_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8940204.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            4311..4463
                     /gene="rpmG"
                     /locus_tag="IV329_001005"
     CDS             4311..4463
                     /gene="rpmG"
                     /locus_tag="IV329_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8940205.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            4487..4657
                     /gene="secE"
                     /locus_tag="IV329_001006"
     CDS             4487..4657
                     /gene="secE"
                     /locus_tag="IV329_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8940206.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            4758..5303
                     /gene="nusG"
                     /locus_tag="IV329_001007"
     CDS             4758..5303
                     /gene="nusG"
                     /locus_tag="IV329_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8940207.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            5380..6267
                     /locus_tag="IV329_001008"
     CDS             5380..6267
                     /locus_tag="IV329_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8940208.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            6440..7459
                     /locus_tag="IV329_001009"
     CDS             6440..7459
                     /locus_tag="IV329_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8940209.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7550..8422)
                     /gene="sdaAA"
                     /locus_tag="IV329_001010"
     CDS             complement(7550..8422)
                     /gene="sdaAA"
                     /locus_tag="IV329_001010"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8940210.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(8435..9103)
                     /gene="sdaAB"
                     /locus_tag="IV329_001011"
     CDS             complement(8435..9103)
                     /gene="sdaAB"
                     /locus_tag="IV329_001011"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8940211.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            9446..9868
                     /gene="rplK"
                     /locus_tag="IV329_001012"
     CDS             9446..9868
                     /gene="rplK"
                     /locus_tag="IV329_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8940212.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            9972..10661
                     /gene="rplA"
                     /locus_tag="IV329_001013"
     CDS             9972..10661
                     /gene="rplA"
                     /locus_tag="IV329_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8940213.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            11071..11574
                     /locus_tag="IV329_001014"
     CDS             11071..11574
                     /locus_tag="IV329_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8940214.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            11627..11995
                     /gene="rplL"
                     /locus_tag="IV329_001015"
     CDS             11627..11995
                     /gene="rplL"
                     /locus_tag="IV329_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8940215.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            12101..12790
                     /locus_tag="IV329_001016"
     CDS             12101..12790
                     /locus_tag="IV329_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8940216.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            12872..14074
                     /locus_tag="IV329_001017"
     CDS             12872..14074
                     /locus_tag="IV329_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8940217.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(14265..15797)
                     /locus_tag="IV329_001018"
     CDS             complement(14265..15797)
                     /locus_tag="IV329_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8940218.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            16031..16732
                     /gene="nagB"
                     /locus_tag="IV329_001019"
     CDS             16031..16732
                     /gene="nagB"
                     /locus_tag="IV329_001019"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8940219.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16986..17699
                     /locus_tag="IV329_001020"
     CDS             16986..17699
                     /locus_tag="IV329_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8940220.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT         5571 a         3222 c         3673 g         5430 t
ORIGIN      
        1 aaactttatt tctacactga tttggctagt ttttatacca atcttctatg gttgatcgga
       61 tttattttcc tttttgcgtt aggaaatgct tggatggaaa gaggggtgcg ttatgacgct
      121 ttataaaacg atttggacat tgcttaaagc aaataaatcg aatttactga taggtgtaat
      181 tatcactgtt gttgccacat tcttttttgc gcaaaatatt gatgttgaaa atacctctct
      241 gcaagatgcg aaaatcgcct tgattagtga agaagagtcg cctgtagtcg atggccttcg
      301 taaatatttg aatgacaagg aaacagtcgt tacattggat ggaacctcac aaaaagaaat
      361 cgatgatgct ctttatttta atcgtgtaaa cattatctta catctgcctg cagactttac
      421 cgaacaagtc gaagtaggaa agatgccgac gatcaagata cagagtcgcc cagatgcctt
      481 ttctaaaaca gttgttacac agcaagtgaa cacctttttg caaacactga ttctgtttca
      541 agaagaagat aaaacaatcg atgcagctat ggaacagaca caaatggcac tctcggttac
      601 tggagaagtt gagctaagtg cgggatatag tcagcggatg aaaaagctgt tgacgggcac
      661 aacctttaat ttcttgtctt atgggttgtt tctatccatt tttagcggtt tcagcgtcat
      721 caatctagca tttaatcgca aagagatcag taaacgaaac cagagtgcgc ccattaccaa
      781 acgaaacttg aatcgcaaag taacctttag tttgattagt tactcgctgc tgttgttcag
      841 tggcttctta cttctaatgg ttctgttagt gatccacacc tcgtgggata taacggtatg
      901 gtatcatatt ttaaatactt tcttgttttt attgccgatc attagctttt cggcttgtat
      961 cacttcattg gtaaaaaact cagaaaccag cggtgggatc aaagatattt ttatcacagg
     1021 cagttgcttt atcggtgggg tgtttgtacc agcagaatac ttgccagaaa tggttagcaa
     1081 aatagcggca ttcacaccaa catattggtt tgttcaaaat aataacttga tcagtgaaac
     1141 actcagttac aatcaaacat ttgcagaatc tttttggttc aatggttgtg tcttagttgc
     1201 ttttgctgcg gtgttttcaa tgatccaatt tattttgggt aaagaaagaa actatcgatg
     1261 gcaacccaat tgggcaaaga attaactgca tagtaaaaaa aggactgtga caaaagtcgt
     1321 gtcacagtcc ttttttccga ataaacggtg ttccagatgt agcttctgac aaaacctctt
     1381 ttattaacac caaaacaact gtttagttgt ggttattgtg atatcaaaag gtcgtttttc
     1441 catttcttct ccatcttctt gaccatgttg cggtgttgtt gaaacgatac gtaatctgtt
     1501 tgttgtgtag tgatggaaat atttcgattt caattgtttt ttctgtataa gtaaaagaat
     1561 gagccacaag attttaaaga aattgattcg ttcgaccacg acaaaatcaa tgactggttt
     1621 agacgcatca gcagttggaa caatcgcgat tcctccgcca aaaaaaggat gcttcgttgc
     1681 tgtacataaa aatgcgtttg aaaaacttaa ttgttttcct cctgcatcta ccagaattgg
     1741 aaatcctttt tgagtgaata aagcacggag aatagagaat aaatacgaaa atgatcctaa
     1801 gttgtatttg ttcaatcgct tttttgtgga agaatgattt gtcgcatgga cgatagctgc
     1861 atctaatcct ataccgaaat tattaacagc tattcctttt ttttcactta ttttttcttc
     1921 ataagccatt atgtggactt tttctggtga ttgagctgct aaaatcagct gaagtccttt
     1981 tttgggattt tttggaaggc ctgcccctcg agcaaaatca tctccagaac ctgctggaat
     2041 ataagcaact gggaattcta cttccatctg atagaaagta tcaagtacct gatgtaaggt
     2101 gccgtcacct cctacgatga tcaaaagggg gaaagtatcg attgttttct gttcttcttc
     2161 tatccaagga atcaatgttt cttttgccaa agtttcagca atctcagcat cgtgtccttt
     2221 gtattcacta taatgaagtg tatagtcaat atgcttattt tttaataccg gtaaaattct
     2281 ttctgctact ttatagccat ttccgctgcc tgccgatgga ttgatcaata tatgataatg
     2341 gaatttcatg atttcctcct gctattcaat aaaacaagat attttaatac tcctgtttgc
     2401 tggttaacca ataattagca agctttttct attttttgaa tgaaagaaac ttgttttttc
     2461 aaaaaaatct gttacaaaat taattcttgg ttattatagc agaagtctag gaaaatgaaa
     2521 aaggtccgaa gaccgaaaaa cacaaaaaag cctagcaagc gcatgatcca ttacttacgt
     2581 aaggagaagt tccaaggttc ggcatgtcca cttagatctt cagctgcttg ctagaccgtt
     2641 ctctagtaag aggttgctca aattcccaca tcctttttta tttatttaag tttcacaaca
     2701 attctgttct tgcagatcct acttcgtctg ctttactggg aattcttatg accccttaca
     2761 cttactttat accatatccc acgattttta gcaaacgttt acattgttta agcttttgct
     2821 tttttaaaag cagataagac ggattgaaat aaatgaaaag tctgttataa ttattgtttg
     2881 tttcacataa ccgcggttca tcaaccatcc cgcgttaaaa aaactaggag aaaagaggag
     2941 agaaaatgca aaaaacgaaa agcagacaac ggttatccgt aatcgttgta aatggcatca
     3001 tcatggctct ttatctagcg ttaactattt tagttgcgcc tgtatcctca gggccgatcc
     3061 agtttcgtat ttcagaaagt ttgaatcatt tagttgtctt taaccgtaaa ttgctttggg
     3121 gagttttagg cggggtcatc gtctataatg ccttctttgg ttttggtatc atggatgtgg
     3181 tttttggtgg cggccaaaca ttgctttctt taggcctgac agctttattg caaaacaaag
     3241 tgaaaaatgt aaagatgcgt ttagtattga acacgatctt ctttaccatc agcatgggac
     3301 tgattgctta tatgttagtt ccaacaggtg gccaagcatt ttggacaacc tatggcacgc
     3361 ttgcattaag tgagtttatc gtcatggctg tttcagcacc attgatgtat tatttagatc
     3421 gcgcgcttga ttttgaaaag cgtgtctaat tataaaaagc gaaaaaagaa agcagattag
     3481 ctgttttctt ttttcgcttt ttgattatgc ttcttggttt tgcaaatttt caatttctac
     3541 cactttataa ttctttttct ctaagtggtt gacgatcgtt tcacatactt tcgcttcagt
     3601 gttagccggt agcgtgatca atgtacgtcg aatgtattcg tctttttcga tatcaagagt
     3661 aatacagctg gcaatgctgc tgtgttttga aatgattttt gcaatagcag ccaaatctcc
     3721 ttgctttcct gtggaagcaa ttgtcagtac atagcttcct tgttctacgc tccaagattg
     3781 agagagcata ttaagcaacg tactatgtgt caaaatacca taaaaatgat tttcactgtc
     3841 aaggacagca atataaggaa gctctttgat cgtaaagaat actttgaaga aagaagtatc
     3901 caggtaaatg aactttgtag cgtttttcaa aagatgagtg acaggaagcg tcatatcacc
     3961 gccatttgct ttatgacgat agatatgcat tttgtaaatg tttccacgga aaatattacc
     4021 tgaagaatct aagatcggca cacaacggta tccggaagtt tctaaaattt ttaatgcttc
     4081 ttctaatgta ctagtctctg taacggttgt cagatctttt ttcttatata caagtgtttt
     4141 taatagcatc gctatacctc caattgtctc attttagctt aatttcaaat taatcatatc
     4201 ataattttat ccaaaacaat agatagttta tttcacaaat tgacaagtaa aaagttacgt
     4261 gatactgtta tgaagtataa gtctgattct cagcaagggg gcagaagtaa atggcaaaga
     4321 aaaaagcagc actcgcttgt tccgtttgtg ggtctcgcaa ctacacaaaa tcagtcagtg
     4381 aaggaaaaag cggcgaacgt ttggaaatca ataagttttg taaatattgt aatcaatata
     4441 cattacataa agaaacgaaa taaacagtag caagggaggt tgtctcatga aatttctaaa
     4501 aagcgtaaaa gatgaaatca aacaagtaac ttggccaagc agaaaacaac tccgtaaaga
     4561 tacgttagta gtcatcgaaa cttctattat tttcggattg atgttttacg taatggatac
     4621 agccatccaa agcttatttg gctggatttt aaaataactc gctagccaca acgtactttt
     4681 agtgctataa tgaagcgaaa gaaaaacttc gggtgtactg aggttttttt attttacaat
     4741 gaaatgagga gccttcaatg gaatcttttg aaaaaaactg gtatgtcttg catacatatt
     4801 caggatatga aaacaaagta aaagcaaata tcgaatctcg tgcgcaaagt atgaaaatgg
     4861 ccgactttat tttccgtgtc gttgttcctg aagaaaaaga aactgaagtc aaaaatggga
     4921 aagaaaaaga aatcgttcat aaaacattcc caggatacgt acttgttgaa atgatcatga
     4981 cagacgcttc atggtatgtt gtccgcaaca cacctggtgt gacaggattc gttggttcac
     5041 acggagccgg aagcaaacca gcaccattac ttccagaaga agtcaatcat atcttgcgtt
     5101 ctatcggtat gagcctgcga caaaatgatc tagaagtaga agttggcgaa gtcgtgaaaa
     5161 tcatcgaagg cgcatttgct ggattagaag gacaagtaac agaagttgac ggagaaaaag
     5221 aaaaattgaa agtcaacatc gatatgtttg gacgtgaaac cagtacagaa cttgatttcg
     5281 atcaagtaga taaaatcgat taataaagag acttgaaatg tcgatcatat gattggcctt
     5341 tcaagtcttt cttttttcta tatggaaagg aagaggtgta tgacagaacc aatcaaaaat
     5401 ttatgcaaac gtgcaactgc agcagcttta gtccttatca gtttgtttgc gttaagtggt
     5461 tgccagacga cgaagataaa tactactgaa gtttctgata aagaaaaaac ggcagaatcc
     5521 agtaaagtga tagaaaaaac cactattcca acactttttt tccacggcta cagcgggaca
     5581 gaaaattcct ttaaagggat gttgcggcgt ttggaaaaaa atcagcaagc agtaaaagaa
     5641 ttggttttaa cagtaggaac agatggacaa atacaagcaa caggagattt gtctggaaaa
     5701 gcagacaatc cgatggtcca agtattgttt gaagataata aaaataacga gtggaatcaa
     5761 gcagaatgga tcaaaacttg tcttttgtat ctgaaagaaa actacggcat agataaagtc
     5821 aacatagtgg ggcattccat gggaggagtt agtgcactac gctatttagg aacgtatgga
     5881 caggatacgt cattgccaca agttcagaca ttaacagcaa ttggcgcgcc ttttaatgat
     5941 tttgtcgatg atagtgcgca gtctctgaca gatgagttag caaaggggcc tgctgtcgtt
     6001 tctaaccgct atcaagatta ccagcaaatg atcggtaata ttcctataac cactcgattt
     6061 ttcttgattg ctggtcaact agatgaaaca gacttgagtg atggaacagt accgttaaac
     6121 agtgcattag cggtctatgc gttgttgaag caacgcggaa atgaaataga ggaaaaagta
     6181 gtgacaggtg aaaatgcctc tcatagtatg cttcatgaaa accaagaagt agatcagctt
     6241 gtaagtcgat ttatttggaa agaatagcga taaaagtatc tgagagtaaa tgtttgctta
     6301 ctttttgtaa gtttagtatg atttatatgt aatcaagaag atgaaagaag gatctactat
     6361 gacttatcat acaagtaatg aagaacatcc aatcgatatt gtaaacattg cctaactaga
     6421 aggtcgtgta aaagagcgca tggaagcagg agcttttggc tatattcgcg gtggttctga
     6481 agatgagtgg acgatgaaag aaaatacgac ttcttttaat acaaagaaaa tcatgccacg
     6541 gatattacgc ggaatcgact cagccgattt gcatacatct gttttcggaa ttgaattaga
     6601 tacaccgatt atccaagcgc catcagcggc ccaaggattg gcacatgaaa aaggggaagc
     6661 tgatacagct aaaggagtag ctgctgctgg atcaatcttt tccatcagca cttatgcaaa
     6721 tacgacaatc aaagacgcag ccgatgcagc gccaggcgca ccgcagtttt tccaattata
     6781 tatgagtaaa gacgacagat tcaatgagtt cattttgaac aaagcagtag aagcaggggc
     6841 taaagctatc attttaacgg cagattcaac gctcggtggt taccgtgaag aagacgtcat
     6901 caatcaattc caatttccat taccaatgcc taacttagct gcgtatagcg aacaaagtgc
     6961 aagtggtaat ggcgaaggaa aaggaatcgc agaaatttat gcagctgcaa aacaaggctt
     7021 gacaccagat gacatcaaaa caatcaaaga aatcacccat cttccagtta tcgtaaaagg
     7081 aatacaatcg ccagaagatg cagtaatcgc tatctctgca ggggcagatg gtatctgggt
     7141 atccaatcat ggtggacgtc aactagatgg aggtcctgcg tcctttgaag tattgccaaa
     7201 aattgccgaa gtagtcaaca aacgtgtacc agtgattttc gactctggtg tccgtcgagg
     7261 agagcatgtc ttcaaagctc tggcaagtgg tgctgactta gttgcgattg gccgaccagt
     7321 gatttatggg ttgaatctag gtggggcaga aggagtaaca tctgtttttg aacacctgaa
     7381 caaagaattg tcgatcacga tgcaacttgc aggaacaaaa acgatcgatg aagtaaaaaa
     7441 tacgaaattg atggattaat aaaaaacgaa gagaagggca attagtcctc tcttcgtttt
     7501 ttgttatgaa gataactaat ctcttaatac tcgaaattaa ggagctttat cacgccctct
     7561 tatttaaaat ctcttccgca agtcgtttag cagttggcgt aacagctaag ccaccttctg
     7621 ctgtttcttt gaaaatcgac ggcatttgac gtccgacttg gtacattgca gcaacgactt
     7681 catccggcgg aatgacactt tcgatacccg ctaatgccat atcagcagag ataaaagctt
     7741 gagaagagcc taaagcatta cgtttgacac acgggacttc aactaagcct gcaacgggat
     7801 cgcaaatcag tcccatcata tttttcaatg taatggctac tgcttgtgct gcttgatgag
     7861 gagtcccatt ttttgcacaa accaaagctg ctgctgccat tgcacttgca gaaccaactt
     7921 ctgcttgaca tcccccttct gcaccactga tagaagcgtt gtttgcgatg actaacccga
     7981 aagcgccagc ggtaaataag aattccaatt gctgttcatg tgtcaaatca agccgatctc
     8041 ggacagccat cagcacacca ggaacaacac ccgcgcttcc ggcagtcggt gtagcacaaa
     8101 tcaaccccat ttttgcattg acttcattaa cagctacagc attgcgtaca gcttttaaga
     8161 ttgtttctcc acttaaaaag tctgcctcat ttaaataatc atctaatttt tttgcatcac
     8221 caccggtaat tcctgtgacg gaggtaacac cttcttttcc ctcgttgatt gattgttcca
     8281 ttactgagag gtttttttcc atgatctcga tgatgtgact acgggaacgg ccactcattt
     8341 ccatttcaac tgcaatcatc aattctgcta cacttggata agcttttgct tgatctacta
     8401 attcttcaat cgataaaaac atttttttag ctccttaatc gaaataattt acgctgtcga
     8461 tatttggcag ctgtttgagt ctttttaccg tttcttctac ttgaggatca tctacttcaa
     8521 ttatcataat ggcattttca ccttttgatt cccgtgtcac tgtcatagtg ccgatattcg
     8581 tattggtctc agaaagtata ctagtcactt tagcaatcat acctggaaca tcttggtgga
     8641 caacgatcaa ggtaggagta cccatgctta aagaaatttt gaatccattc aattcagaaa
     8701 tctggatatt gccaccgccg atcgaaatgc cagtgactga aagctttcgt gttccttttt
     8761 ttagaagcat ctttacagag tttggatgtt cggctttttc actcttagga acaaacaaga
     8821 cttccatacc ggcttcatat gccaatttca aagaatcagc taaacgttca tcatcgggtt
     8881 ccatccctaa caggccgcca actaatgcaa tatctgttcc gtgcccccga taggtttttg
     8941 caaatgattc atatagataa atatctacag aatcaggctg ttcgccaaat atactacgta
     9001 tgattttccc gattctagca gctccggcag tgtgagagct acttggacca atcatcacag
     9061 gtccaataat atcaaagaca cttctatacc ttaaatcttt cattttctcc acctcatttt
     9121 gttatgtagg aatctttgta caagggtcta aaaaaccgtg ttattgtttg ctttcatctg
     9181 ttgaaccatg ctagcgcaag tgtaacataa aagaagaaat gagaacgaaa aattgttgtt
     9241 tttttagaat aaatttagcg tttattcaag caaaatgtaa aaatagcaga aaaaaatctt
     9301 gaaaaaacaa ataaatttgc tatactgtac cagtgtgcat tgttttgcac attatttgtc
     9361 tggatttttt cagatcagat aaaacgtggg aggagaaata tgcatctcca attaaccaca
     9421 tcacggactt aaggaggtat gtctcgtggc aaagaaagta gaaaaaatcg ttaaattgca
     9481 aattcctgca ggtaaagcaa ctccagctcc accagtaggt cctgcactag gtcaagcggg
     9541 tatcaatatc atgggattca caaaagaatt caacgctcgt acagctgatc aagcaggttt
     9601 gatcattccg gttgttattt ctgtatacga agaccgttca ttcacattcg ttacaaaaac
     9661 accgccagct gctgttctat tgaaaaaagc agctaaaatc gaaaaaggtt caggcgaacc
     9721 aaacaaaaat aaagttgcga aagtttctag cgatcaagta agagaaatcg ctgaattaaa
     9781 aatggaagac ctaaacgcag ctaacgttga agcagcaatg cgcatggttg aaggaactgc
     9841 acgaagcatg gggatcactg tagaataatc tatacaccct aagacagcag cttctcagtt
     9901 ggctctatat tgaaagatat agaacacgtg ggaggtacaa ccgttataac cacaatcaag
     9961 gaggaaccaa aatggctaaa aagagcaaaa aaatgcaaga tgcattaaaa aaagttgatt
    10021 caagtaaagt ttatcctgtt gccgaagcag tagctttagc aaaagaaaca aacattgcaa
    10081 aatttgatgc aacagttgaa gttgcatacc gtttgaacgt agaccctaaa aaagcagacc
    10141 aacaaatccg tggtgctgtt gtattaccaa acggaactgg taaaacacaa actgttttag
    10201 tttttgctaa aggagacaaa gcaaaagaag ctgaagcagc tggtgcagat ttcgtaggtg
    10261 acgacgacat ggttcaaaaa atccaaaacg gatggtttga ctttgatgta gttgttgcaa
    10321 cacctgatat gatggctact gtaggtcgtc taggacgagt attaggacca aaaggcttaa
    10381 tgccaaaccc taaaactggt acagttacaa tggacgtaac aaaagcagta aacgaagtga
    10441 aagctggtaa agtaacttac cgtgtggaca aagctggaaa tatccatgta ccaatcggaa
    10501 aagtttcatt tgacgatgaa aaattagttg aaaactttgc agcaatccat gatgtattgc
    10561 taaaagctaa accatcagca gctaaaggtc aatacatgaa aaatgtaacg atcacaacaa
    10621 catttggacc tggtatccat gttgaccaag cttctttcta attaaatatt agaatgttga
    10681 ttgatccaac tgaaaaataa ttcttgactc aactagttta gcatgctaag ctagttgagg
    10741 ttaataatta acttgtaccg aagacagtag gtggcatcaa gccttaattt cctaccgagg
    10801 acgtttattg aatatattca atagtccctc tatgtctgcg gtggcataga gttttttatt
    10861 gtgaatcact atgaacgaag tgaagagatg atgaacaata ctggttgagc aaagcgaaag
    10921 gagtttcctt tcgagcaaag cgagtagaat cactatgaac gaagtgaata ggtgatgaat
    10981 aatactggtt gagcaaagta actataaaag tgacttgcct tttcagtgca aaaaactctt
    11041 ccatcaaaat tcaggaggtg aaactcaaaa atgagtgaag caattatcgc aaaaaaagca
    11101 gcaatcgttg atgaagtaag cgaaaaattt acagcagctg catcagtagt agtcgttgac
    11161 taccgcggat taacagttga tgaagtaaca cgtttacgta aacaacttcg tgatgctaac
    11221 gtagaaatga aagttatcaa aaattctatt ctttcacgtg cagctaaaaa agctggcctt
    11281 gaaggcatgg acgaagtatt cactggacct acagctgttg cattcagtaa cgaagatgta
    11341 gtcgcaccag cgaaaatcat ggacgaattt gctaaagaag caaaagcatt agaaatcaaa
    11401 ggcggtatca tcgaaggtaa agtctcttca ttggaagaaa tcacagcatt ggcaaaattg
    11461 ccaaatcgcg aaggacttct atctatgttg ctatctgtac tacaagcgcc agtccgcaac
    11521 gtggcttacg ctgtcaaagc agtggcagac aaagaagatg gagatgcagc ttaattgccg
    11581 cataaagcat taaataaaaa agaacaatta aacggaggaa attataatgg cattgaacat
    11641 tgaaaacatc gttgctgagt taaaagaagc aactatccta gaacttaacg acctagttaa
    11701 agctattgaa gaagaatttg gcgtatctgc tgctgctcct gtagctgtag ctgctgctgg
    11761 tggcgctgct gctgctgaag aacaaacaga attcactgta gaattaactg cagctggaga
    11821 ccaaaaagtt aaagttatca aagcagttcg tgaagcaact ggtttaggct tgaaagaagc
    11881 taaagctgta gttgatggcg ctcctgcacc agttaaagaa ggcgtatcta aagaagaagc
    11941 tgaagaattg aaagctaaac ttgaagaagt tggcgcatct gtaactgtta aataattttt
    12001 actatttaaa agcactgaga tcctatgttg gatttcggtg cttttttgtt tttatgttaa
    12061 actattattt aatgaatata gatttttttc aggaggaaat atgaaaaaga aaataatccg
    12121 attgatattg atctcgttgg gaatcattct gatactggta gtgggctatc ggttgtattt
    12181 agaatatcag acagacatcc aattattttt aaatccgaga gcgagtaagg cagagctact
    12241 cgcacatatt cgttcgcatg ggacttttgc atcgatactc ttgatcgtat tgacagcggt
    12301 gatgtgtgct gttccaggta ttcctacttc agtagttgga gtcattgctg gtttgagctt
    12361 tggaccactg acaggtgcga tcatcaacat tttgggtaat gctcttggta acatcactgc
    12421 aattttcctt atgcatcgct taaaattttt agataagaaa accgaaacga atcattgggt
    12481 gcaagcaatc cgacaaatga aacatccaaa aataggtgta atgttaggct atatgatacc
    12541 ggttattcca tcttctatgg tcaattttgc tgcagatgcg atgaaattgc ctctacagca
    12601 aatcatattg tcgatcgtga ttggagtcat cccttcgtct gtgttgtatg cgtgtggtgg
    12661 agaagctctg tttcacggat ataacaaaac tgcagtcacg ttggttgcaa gtgttactgt
    12721 gctagtaggg ttagttgtct tcatttataa agatcgcaag aaaaaacaag gaaaacaaaa
    12781 gcacaaataa taagtgtggc gaatagttgt atttttctat ttccaatctt ttccataagt
    12841 tgtaaactaa gataggaaat gggggaaatc aatgaagagt aatgaaaaaa aacctttaac
    12901 aacttcgctt attttactat tatcaatcac ttgtggcgtt gttgttgcca atatgtatta
    12961 catccagccg attggaacaa aagtagcagc tagtttatct gttagcacaa gtgcagtcgg
    13021 gatattgacg atgttgaccc aattaggcta tgcgttaggg ttactgtttt tagttccttt
    13081 aggggacgtg gtggatcgtc caaaattgat cattcgaatg gcagcgttat ctgccgtctc
    13141 tctgttggcc gctttttttg cgccttcgtt tacgttattt gcatgcgctt cttttttaat
    13201 aggattactt tcgatcgtgc cgcaaatcat cattccttac ggtgcggtac tagcgggacc
    13261 aaaagcaaga gggaaggtca tgggtacatt gcttagcggt ttactcgtgg ggattttgct
    13321 ttctcggacg gtatctggta ttgttgccag tcttttttct tggcggatga tctatttatt
    13381 tgctttatgt gcagtcggaa ttcttacact tctgctttac tggaaaatgc cgcgttctgt
    13441 ccagcaaagt agtacagctg tttcatacct tgattcactt aaaagtttgc cgcatttagt
    13501 tgcttctcaa cgtctattaa gagaagcggc agtcagtgga tttttcatgt ttggaacatt
    13561 ttcgatcttt tggtctacgt tgatttttta tatcagcagt cccgcctaca attggggaac
    13621 attcgaagca gggatattag cgatttttgg tttgtctgga gcagttgctg cacctatcgt
    13681 gggacgactg gcagattctt attctgaaag aaaaatcgtg tatatgggac ttgttatgca
    13741 aacaataagc tttattgctt tattggttgg cggaagccat ttggccattt tgcttttagc
    13801 tattatttta ttagacgtcg ggaatcaatt tggtcaagtc gccaatcaaa cacgagtaca
    13861 agggctaggt gaagcagcaa gcaaccgaaa taatactgta tttatgttta tgtactttat
    13921 tggaggtgcg gctggttcat tgctagggac gacaatgtgg cagcagtatg gctggttagg
    13981 tgtaacggtt agcgggcttg tttttcaagg atgtgcgttc ttctttcaaa cagttgtttt
    14041 taaaggaaag agaaatctcg aaaataaaaa atagacgaaa agaggttgtg aaaagctgtt
    14101 ctgcatcaag taataagaat agccaaataa aaatagctcc tcatattttg ccgttttttg
    14161 agctaatacc gaaggtcagc taattggcca ccatttattc agaagaagga gttgtgacaa
    14221 aacttttgtc acagctcctt tttttcaact attcaatcaa acgtttattt tggttcaatc
    14281 tcaatgactc gttgcacaag atcttgataa aattcttgtt catgagagac gatgattact
    14341 gttcctggaa atttctgtat cccgtcttgt aagctttctt tcgtttcttg atcgatatgg
    14401 ttcgtcggtt catcgagaat cagtaaatta ccttggttca ttgtcaactg tgcaagtttc
    14461 accttagttt gttccccacc acttaatgta gtgagaggtt cacttgctaa ttgattaact
    14521 aaccctgctt tagctagttg gcgacgtaat tctttgatag tcgcttttgg aaattgactg
    14581 cttaaatatt gtagtggtgt ttctaatgga ttttcccagt acagatcttg tgaaaagtaa
    14641 ttgatttttg tgttatctgg gaagtggaaa tcgccactta ttttttcaat ctcaccgatc
    14701 agtgttttta ataaagtaga ttttccaatc ccgttgaagc ctcgaatggc aatattttca
    14761 ccaaagcgga tggtcaaatt gatgggtgca agtagcggtc gatcatatcc gatgactaaa
    14821 tcagttgttt ccactgctaa tgtcgtgaca ataggcgaga atggaaaatc aaagacagct
    14881 ttacttgctg aagctggagg cgtcagccgt tcgattttat ctaattgttt ttgtcggctt
    14941 tttgccatcg ttgatcggga accggctttg tattttcgaa tataagcttc ggtcttttcg
    15001 atttgttttt gttgagcatg atattggcgc aaatagcttt cgcgattttg ttccttttgt
    15061 ttcaatgctt gttttagatg gccagtatac ttagtaatct ttccaaactc gatgtctgcg
    15121 atatgggtgg tgatctgatc aagaaattct tggtcatggg aaatcacgat atacgcaccg
    15181 tcaaaatcct gaagaaactg cgtcagccat tggatatgct gatcatctaa gtgattggtt
    15241 ggttcatcta agatcagaac atctgggttt tctaaaagta gttttgctaa aatcaccttt
    15301 gaacgttgtc caccacttag atttttcaac agtgaatcta aacctaagac atcgatcccc
    15361 aacccgctgc tcatctcagc aactaaggtg tcgatttgat aaaacactcc ttggtctaat
    15421 tccgtttgga gtttcccagc tttttccagc aaggaatcat cgaaggtctc gctatatgtt
    15481 tggtaaagaa aagcaatttt ttcttccttt tcgtattctt ttgcgtacgc agtttttaga
    15541 aattcttcga ttgttaatgt ttcatcgact tccacatatt gatcgaggta acctttgtga
    15601 atattttttt gccaatggat ctgtccgtca tctggtaaga tctcaccagt gatgatttta
    15661 atcaatgtac ttttaccggc tccgttttgt ccaatcagcc caagatgttc gccagcattt
    15721 aattgcagat cgacaccttc atagaggatt ttgtcgccaa attgctgggt aatgttgttt
    15781 aatgtcagta tactcataat aactcctttt tcataaaaaa actgcagaca taaagcgtcc
    15841 acagtgaatc acttgcttgg ttttcatgca acatggattg tttgattgaa ccatcttatg
    15901 aaacctaaca cggattccta tttattgtca agaaaatgaa aaaaatgcaa tttggtctat
    15961 actaattatt gtaaaaagaa aaaaagaatg ctataattgg gctataccaa aatataaagg
    16021 agcaatcact atggaaatca ttcgtgtaaa aaacgctgaa gaaggcggaa aaaaagcatt
    16081 tgaaatcatc aaagaaggta tgaacaaagg tgcaaaagta ttaggattgg caacaggaag
    16141 cacaccagtt actttatata aagaaatgac ttcaagcgat ttggatttca gtgaaatgac
    16201 ttctgtaaac ttagatgagt acgttggact aggcggttca gatgaacaaa gctaccgtca
    16261 tttcatggat gtccaattat ttgataaaaa accattcaaa gaaacgtttg taccagatgg
    16321 aaaagcagaa gatctggaag cagcatgcaa aaagtatgat caaatcatcg atgcacatcc
    16381 aatcgatatc caaatcttag gaatcgggca aaatggacat atcggtttca atgaacctgg
    16441 tgcttcattt gacggaacga cttcagtagt tgacttaaca gaatcaacaa tcaatgcaaa
    16501 caaacgattc ttcgataaag cagaagatgt accaacaaaa gctgtttcaa tgggaatcgg
    16561 ttcaattatg aaaagtaaag aaatcgtctt gatggcattt ggcgaaagca aagctgatgc
    16621 aatcaaaggg atgattgaag gacctgtaac gaatcatttg ccagcaagtg tattgcaaaa
    16681 tcacgacaat gttgttgtga tcatcgatga agaagcagca agtaagttgt aattttcaat
    16741 agtaggctaa agaggttgtg aaatcagcgt tttgacctga gtattagaga agacaaagga
    16801 aaaatagctc cccatatttt gccgtttgtt gagtaatacc gagggtcagc tgattgaaca
    16861 ccgtttattc ataaaaagga ctatgacaaa aattttgtca cagtcctttt ttcgtaagag
    16921 gtaaaaattg cacttttttt gtctgctcct ttcagacatg atatattgga ctggagggtg
    16981 tttagatgaa tgatatggta ttgacaattc cagagattat tttacgttta gggatcgcta
    17041 tgctatttgg cggagcaatc ggttttgaac gacaatataa aaatcgtcca gcaggtatgc
    17101 gtacgcatat tttagtttgt atgggagcat gtatcattgc attgatccaa gttgagatcg
    17161 ctagtggtgc attaagagat gctttagagt atccacagct tgctggaacg attcgttcag
    17221 atgaagcaag gctgattgct caagtggtga gtggtgtcgg tttcttaggc gcgggaacga
    17281 tcatcgtcac gaaacaatcg gtgacgggac ttacgactgc cgcttcttta tgggcaattg
    17341 ctggattagg gatcgccatc ggcatgggtt actattccat tgcgatcatc agttttatcg
    17401 ggattgctat tgcactcaca gtagtgaaac gcgtgatcca tgtacctacg actaaaaagt
    17461 tagaaatcca atttatccat agaaaagaaa ccaaagattt tcttaccgat tattttgaag
    17521 agaaaaatat cgtaatcgaa gatgtgaatt ttgacgtaaa attcgttgat gactaccgag
    17581 tgtacaaaaa tatctatacg attgatcttc caaaagggct cacttatgcc gatgtgatcg
    17641 aggagttgtc cgtacacaaa aatatcacaa agttgcattt agtcagtatt gcgcaataaa
    17701 tgatctgagg ttgtaaaata gatgtcctac atcaagtaat aagcccagcc aaataaaaat
    17761 agttccttat atttttcgtt tggctgggct tattacgaag atgcaagctt ttgaacaccg
    17821 tttatttgga aaaagggact gcagtataat ttttgtcact actccaaacc gtgtttttgt
    17881 ttaagtactc gtctac
//
LOCUS       DACDKL010000038        17872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDKL010000038 DACDKL010000000
VERSION     DACDKL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.038"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            352..1041
                     /locus_tag="IV329_001021"
     CDS             352..1041
                     /locus_tag="IV329_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940221.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IV329_001022"
     CDS             1028..2236
                     /locus_tag="IV329_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8940222.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IV329_001023"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IV329_001023"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8940223.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IV329_001024"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IV329_001024"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8940224.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IV329_001025"
     CDS             complement(6339..7034)
                     /locus_tag="IV329_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8940225.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IV329_001026"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IV329_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8940226.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IV329_001027"
     CDS             complement(8233..8424)
                     /locus_tag="IV329_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8940227.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IV329_001028"
     CDS             complement(8445..9335)
                     /locus_tag="IV329_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8940228.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IV329_001029"
     CDS             complement(9322..10089)
                     /locus_tag="IV329_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8940229.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IV329_001030"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IV329_001030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8940230.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IV329_001031"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IV329_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8940231.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IV329_001032"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IV329_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8940232.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IV329_001033"
     CDS             14705..15043
                     /locus_tag="IV329_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940233.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IV329_001034"
     CDS             complement(15211..15969)
                     /locus_tag="IV329_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8940234.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IV329_001035"
     CDS             complement(15987..16805)
                     /locus_tag="IV329_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8940235.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IV329_001036"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IV329_001036"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8940236.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IV329_001037"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IV329_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8940237.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
BASE COUNT         4985 a         3701 c         3265 g         5921 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac ta
//
LOCUS       DACDKL010000039        17765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDKL010000039 DACDKL010000000
VERSION     DACDKL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.039"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..1560
                     /gene="arcD"
                     /locus_tag="IV329_001038"
     CDS             139..1560
                     /gene="arcD"
                     /locus_tag="IV329_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8940238.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1574..2692
                     /locus_tag="IV329_001039"
     CDS             1574..2692
                     /locus_tag="IV329_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8940239.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2752..4044)
                     /locus_tag="IV329_001040"
     CDS             complement(2752..4044)
                     /locus_tag="IV329_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8940240.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            4194..4754
                     /locus_tag="IV329_001041"
     CDS             4194..4754
                     /locus_tag="IV329_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8940241.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            4883..5260
                     /locus_tag="IV329_001042"
     CDS             4883..5260
                     /locus_tag="IV329_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8940242.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5193..7265
                     /locus_tag="IV329_001043"
     CDS             5193..7265
                     /locus_tag="IV329_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8940243.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(7476..8423)
                     /locus_tag="IV329_001044"
     CDS             complement(7476..8423)
                     /locus_tag="IV329_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8940244.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            8669..9133
                     /locus_tag="IV329_001045"
     CDS             8669..9133
                     /locus_tag="IV329_001045"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8940245.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(9195..10238)
                     /locus_tag="IV329_001046"
     CDS             complement(9195..10238)
                     /locus_tag="IV329_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8940246.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(10615..11580)
                     /locus_tag="IV329_001047"
     CDS             complement(10615..11580)
                     /locus_tag="IV329_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8940247.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            11814..12659
                     /locus_tag="IV329_001048"
     CDS             11814..12659
                     /locus_tag="IV329_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8940248.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            12850..14037
                     /locus_tag="IV329_001049"
     CDS             12850..14037
                     /locus_tag="IV329_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8940249.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            14043..14399
                     /locus_tag="IV329_001050"
     CDS             14043..14399
                     /locus_tag="IV329_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8940250.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            14401..14739
                     /locus_tag="IV329_001051"
     CDS             14401..14739
                     /locus_tag="IV329_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8940251.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            15134..16168
                     /locus_tag="IV329_001052"
     CDS             15134..16168
                     /locus_tag="IV329_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8940252.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            16161..16847
                     /locus_tag="IV329_001053"
     CDS             16161..16847
                     /locus_tag="IV329_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940253.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            16871..17719
                     /locus_tag="IV329_001054"
     CDS             16871..17719
                     /locus_tag="IV329_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8940254.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         5903 a         3261 c         3769 g         4832 t
ORIGIN      
        1 cttgaaatac ttgtgtaaaa agttcataat atccaataat atgaataaag ttgctttttg
       61 ttaagcaaga gcagttgcaa aatagaaggg aatcttttgc aagtgatttg ttcaatttta
      121 taagtgaggg tgatggaaat ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt
      181 gctctggttg tcagttcgtc aatcggaagc ggtgtgtttg ggatcacaag tgatctggct
      241 agttctgcag ctccagggcc agcgatcctt tcatggataa tcgttgggat tggtatttta
      301 gctttggtat tatcgctgaa tcatttagga gaaaagcgtc cagatctaga tggtggtatt
      361 ttcggttacg cggaagcttc atttggtaaa ttaggtggct tcatcagtgg ttggggttac
      421 tggttgtctg cgtggctggg aaatgttgct tttgcaacga tgctgatgag cgccatggga
      481 gaatttttcc cgatattcaa aggtggacaa aatgttcctt ctattttatt tgcaagtatt
      541 tttatttggg gtctgacttt attagtaaac aatggagttg aaggagcgag cgtcatcaat
      601 acaatcgtga cgatctgtaa attagtgcca ttattcctat ttttaatatt tgtatttatc
      661 gcatttaaag tgaatatatt cactgctgat ttttggggta acgtatctga taatctagtg
      721 aatggaacag ggcagcaagg aacgatctgg ttgcagatta aaggttgttt gatggttatg
      781 atgtgggtct ttgtagggat cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt
      841 tcagaagccc agcaagcatc gatcattggt ctgctttgtt tattgtttat ctatatcctt
      901 gcttcgatct tgccatacgg cgtcttatct caagaagagt tagcaactat ttcacaacct
      961 gctatggcaa atattttaaa aggaatcgtg ggaaattggg gagcagcgct gatcaatatc
     1021 ggcttgatta tttcgatcat tggttcttgg ttgtcttgga cgatgctccc agcagaaaca
     1081 acgatgttga tggctcgaga cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa
     1141 aatgccccaa cttattcgtt agttgttact gcaatcttga ctaatctatt cttattgact
     1201 ttcttagtta ctgattatgc ctatcaattt gcctattctt tatgtacagc ggcaattttg
     1261 atttgttatc tacttgtcgg gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa
     1321 aaacaagtag tgatcggtgg gattgctgta gtttttgaat tgatgggcat cattttagca
     1381 ggattttctt atgtcctttt atgtagtatt gcctatcttc ctggtttttg cttctactgg
     1441 atcgcttgtc gtgaaaccaa acatcaaatt acgggaaaag aaaaagtaat gattgggctg
     1501 atttgtgtgg ccgcactgct ttcaatcggg ttgcttgctg taggctggat caagatataa
     1561 gggggaaaga aacatgaaaa tcgcaaattt cggtgtagaa gaatggctaa ataaatggga
     1621 aaagaaagcg gtatatgata ttgcacaaag ctcgatcgaa gcattgacat tagacgaact
     1681 cgttggctta gatgggacta gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt
     1741 ggactatggc tggatcgaag gatcagatgt tttcaaacaa gaagtcgctt ctctttatca
     1801 aaccgtcgat ccggaaaaca tcttacaaac gaatggagcg actggtgcta atcttttagc
     1861 tctatacgcg ttagtggaag caggagacca tgttgtttcg atgcttccta cttatcagca
     1921 attatatgac atccccaaat ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga
     1981 agaagattgg cagtttgatt tagaacagct agaagcctta gtcaaacctg aaacaaaaat
     2041 catctgtttg aatagtgcaa acaatcctac aggcacattg ctagatcgga aaactttaga
     2101 aaaaatcgca gaaattgctc gtacagtaga tgcatatgta ttgatagatg aagtatatgc
     2161 gcctttaacg gataaaggtg aattcttgtc gatcgtagat gtctatgaaa aaggtatagc
     2221 aaccaattcc ctatccaaaa cctactccat tccaggtatt cgtattggct ggacagctac
     2281 tggacctgag ttggcagaag tttttcgaaa gtatcgagac tacacgatga tttgcggcgg
     2341 agtcctgtca gatgatcttg ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg
     2401 aaacaaaaag atcattacag agaaccttgt gattttaaaa caatgggtag ccaatgaacc
     2461 gaaagttgaa cttgtcgctc ccaattacgt ctctacttct ttcattaaat tagccatcga
     2521 agaagatgac cagacatttt gtatcaattt attagaagaa acaggagtac tattagtacc
     2581 aggaagtgcc tttgatttac cgaagcatgc ccgactaggt tactgttgta aaaaagaaac
     2641 cttggaaaag ggattattcc tcttatctga gttcttgaaa aaacaggcct aaacaaaaac
     2701 actgcaagga cagtctgtga caactgttca agcagtgttt ttcatttacg cttattgtct
     2761 atgagaagga gagtgccctg aagctatgga gactcctgaa gctttttctt ctgcagtttc
     2821 ttgctgatgg atctcttgtg tattgtgtcg tacaagcaag aagttgacca aaacaagaag
     2881 agaagtcgaa agaaagacgc cacgataacc aaatgcactg gaaacactgg agccgatcat
     2941 aggccctact acgttaccag ttgcttggaa agattggtta taactgaaga tccgtccagc
     3001 agcttgatga ggcgaatatt ttgtgatcaa tgcttggaca gcagggagca gacaagcatc
     3061 agagatacca atcaaaaaac gtaaggcagc taattgccaa acattcgtga cgaatgccat
     3121 tgggatataa accagaatcg caaaacccaa accaatcgca agtattcttt cactcccaat
     3181 cttgtcacct aagcgaccaa atcttggagc ggctatcaaa gtggcaatcc caggtatcga
     3241 agcaatgatt ccgctgatca acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta
     3301 cagactgatg attggactaa tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa
     3361 cataccgatg acgacgtgtg gatatttcaa gtctttgaaa atctgtttgg cagaaaccat
     3421 gttttctttt ttcactggga caaatttttc atggacgaaa aacagactca acaagaagac
     3481 gaaaaataag atgatccctg taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga
     3541 gatcccgcca agaagcggtc caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc
     3601 aagaacttga ccactttttt cttttggcgt cccagttgcg acaagggcag tggcattact
     3661 gatataacca gaaaaaatcc cttgaagcag acgcaatgcg actaattgat acacactagt
     3721 cacgcaaccc atcaaagaaa tgacgattgc cattcctaat gaagcacgca acagcatcaa
     3781 ttttcttcct ttttggtcgg ctaattttcc ccaccaagga gagataagcg tagtcaccaa
     3841 gaaagtagat gaaaaagtca atccgctcca aaagttcaat tgtgacgtac tataattacc
     3901 taacgtatct atgtaaagtg acataaaagg catgactaaa ctaaatccga taccagccat
     3961 aaatgttcca aaccataaga caaacaagtt ttgttcccaa gtttctcttt tcctaaataa
     4021 tttttccttt attcttgcag ccaattagac tccttctttc tgaaaatttt catctacagc
     4081 tctttattat aaactacaat atgtagttta tcaatgaaag tctctttttt ctctttcttt
     4141 ttcgtggtaa actgaaagag tgaaggattt attttatgag aggaaacatg gaaatgaaaa
     4201 aaagaaatct gtctcaagag aaaatcatcg actgctttcg agaactagca gaagagatgg
     4261 atgttcagca aataaccttt cagcatttag ccaaacgctt ggatatcaag gctccctcgc
     4321 tgtataacca tttcaaaaat attcgtgatg tcaaaacagc gctaacagcg aaacttctgc
     4381 aggaacttaa tgaccagctg cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc
     4441 agatctatgc agaaacttat cgatcctttg ctttttcaaa tcaagcagtg tatgagttac
     4501 tgatcagtgt accgcatacc aatgaagctg ttttattaga tggaatccat gaaacgaatc
     4561 agatcatttt gcagatattt gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa
     4621 gcagggaact tcgtagtatg atccacggtt atctttcctt acgtttttta ggatacttta
     4681 caaaagaacc aacaatcgat ccagaagaca gctatttttg gatgatcaat aactttatcg
     4741 ctactttgcc ttaaacaatc agattcattt gtaacaattt taagatgttc attaaatgac
     4801 ccttttataa gagaaaattc ttgcaacttt cttgaaaagt gactaagatg agtctagaaa
     4861 agctgaaaga aggagcacga gtatgtatca agtaattacc atgttcgggg acaatgagcc
     4921 ttggtggttt tttgaagact gggaagaaga tattgaagaa gaagaaacat tcgagtcttt
     4981 cgaagaagca cgacaagcgt acgaaaaaca atggaacgaa atccatcaaa attatgaata
     5041 tatcaatgca aaaagtaact ttctcagcgc cttttggaat gacggagatg agcgctggtg
     5101 tgaagagtgt gacgatgatt tacagcaata caagggactt gcgttgttga aaaatcatca
     5161 gcccatcaca gtagaaagca gaaaggaatt ttatgaaaca actaattcta gcggaaaaac
     5221 caagcgttgc gaaagaccta agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt
     5281 atgaaggacc caaggtgatc gttacttggg cccttggtca tttattagga ttgaaaatgc
     5341 cggaagatct gaataaggaa tggcagagct ggcaaatgga cacgttgcca atgatcccta
     5401 aaaaactagg aatcaaacca cttcctaaaa caggacatca attaaaagca atcaagcagc
     5461 tggctcagcg aaaagatgtc tcagaagcag tcattgcgac agatgccgga agagaagggg
     5521 aacttgttgc tcgctggatc ttagagtatg tgcatttcaa taaaccagtg aagcggttat
     5581 ggatctcttc tcaaacagat aaagcaatca aaactggatt caagcaacta agacctgcga
     5641 aagaatatga tgcattgtat gaatctgctc ttgcccgggc aaaagcggac tggctggtag
     5701 gattgaatgt gaccagagcc ttaactgtga agtacgcgga taatttatca gcaggccgtg
     5761 tgcagacacc gacactagca ttagtgagag accaagaaaa gaaaatcgag cagttccgtc
     5821 cacaaacgta tttcacgatc acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa
     5881 aaaatcaatt tgctttaaaa acacaagaag aagcacaaaa acttgttcaa ttgatcagta
     5941 caagcaaagg aactgtcaca gaagtccaag aaaaattaaa aacggaatct gcgccattgc
     6001 cttatgattt aacagagatc caaagagaag ccaatcaacg ctatggctat tctgcaaaga
     6061 aaacattaag tcttgtacaa agtttgtatg agacccataa gatcgttact tatccgcgta
     6121 cagacagcaa atatctgaca aatgacatga aaagtacgat gaaagaacga ttgcaagcag
     6181 tcagcgactt tgctcctgag gtcaagcagt atttgaaaaa cggagccatc gtccaacaga
     6241 caaaagtatt ccaagacagt aaagtgaccg atcaccatgc acttttgcca actgaaaaca
     6301 gagcacgtta tgaaaaatta agtaatgaag agcgaaaaat ctatcaaatg attgtctctc
     6361 gttttttagg actgtttgct caaccccata aagtcagcca aacaaaagtt acggttgaat
     6421 ttggtaaaga acgattcatt tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg
     6481 aaactgaaac tgagacggaa acagtcaaat gggaaaaagg aatgagaatc aatcccgatt
     6541 tcgccatcaa aaaagaacta tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg
     6601 gtcaaatgga aaaatacagt ctgggaaccc cagctacgcg tgcagagatt atcgagaaac
     6661 taatcaaatc tgaactgatg gaacgtaccc ctcaaggact gcaagtatct cctaaaggta
     6721 agcaattatt agtattggta aatccctctt tagtaacacc agatttgaca gaaagctggg
     6781 aaaaagacct tgaagctatt gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa
     6841 tcgaaaaaga aacaaaacgc ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag
     6901 atttctcgat tacccaaaag aaatgcccag aatgtggggc caatctgcgc gagaaaaaca
     6961 cacgtgacgg caaaatctat gtctgcacca attcagattg caactaccgc agaagaaaag
     7021 atccaaaagt ctctaatcat cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag
     7081 gtaaaaacgg tgcttatttc cgttgtaaat acgatggaac gacagaaaaa ttgcttgata
     7141 aaaaagagca gaagaagaaa atgaccaaac atgaagaacg acgcttgctg aaaaaatatg
     7201 cacaagcaga agaaccagaa gaaagcccat tagcagcagc actaaaagca gcaatgaaga
     7261 agtaataaga tatgaaagag gttgtgatag aagtggtcag cttcaagaaa taagaaggaa
     7321 tttacgaaaa ttgcttctca aatttttgtg aaatttcggc ttatttccga agaagctact
     7381 tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca cagtcctttt
     7441 tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt caattcattt
     7501 ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt ttctaagaag
     7561 attttcccaa tatatttccc gatgattcca agacataaca attggattcc accaatgaac
     7621 aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa caatgcccgc
     7681 aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc gataaatgaa
     7741 gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta gttgaataat
     7801 ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata agaaatatat
     7861 tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta ttctgttaat
     7921 tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc gccatcgacc
     7981 atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt
     8041 atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat atcagaagta
     8101 gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc aacatccatc
     8161 acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc ttctttccca
     8221 aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag cttcttgatt
     8281 gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa ctcatgggca
     8341 cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc attaaaacat
     8401 gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt atatatggtt
     8461 tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt acgtaaaaga
     8521 acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa
     8581 cttttaagaa cattttttat tagttggcaa gtagttttgg atagagaaaa aggaggaagt
     8641 tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg gctttttatg
     8701 gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt cagaaagcga
     8761 atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat attttgcggg
     8821 aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa
     8881 tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata aagaagtagc
     8941 cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc gaaccaatcc
     9001 attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac tgacaggata
     9061 tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt tagaaggata
     9121 tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat cacaactgtt
     9181 ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat cactgtttgg
     9241 agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat gaattttgca
     9301 aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct gctgataaaa
     9361 tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa acaaaagatc
     9421 acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt atcagttggc
     9481 gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata gacaacaaca
     9541 acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac agaattgtca
     9601 agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc caaaagtgca
     9661 cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag agtcaacatc
     9721 atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc ctctttcgca
     9781 tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt tcctccagcc
     9841 atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta agcaccgatg
     9901 atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat ttgagcaatc
     9961 gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag tttgctttca
    10021 gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc taataaaata
    10081 cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc aagaagcctg
    10141 ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg gaccgctgaa
    10201 tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc tttcgtcaat
    10261 aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac aaaagaacaa
    10321 gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt tccagaagta
    10381 gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
    10441 ttcttatttc ttgaagctga ccacttcttt tacgacctct tggtaaacgg tgttcaaaag
    10501 cttgcatctg cggtaataag cccaaccagt acaaaaacat gaacagctgt ttttgtttgg
    10561 ccgttcttat tacttgatgc agaacagctt tttcacaacc tattgaatcg attattaagc
    10621 gattttttta gaaacaaatt ttccatgttt gctatgttct ttaattaaga aacgtacgta
    10681 atgagcaaga acgtagaaga caactacact tgcaaacaga cattccgcaa cttttaagaa
    10741 atccaatacg ctactataga tacctaaact gttgaagtaa tgaacagtag taaatatagc
    10801 tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag cttggataaa aacgaagctt
    10861 gaacaaacgt ggaagcatag acagcacaac gaaatacaat acttgagata aagcgaacaa
    10921 tgctacgaca agataaaaat taggttcagc aaagttttta agataacctg ttaggcaaag
    10981 agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc ggatgagcca tatttcttac
    11041 gaaaaagaca cgtttgatga tgatcggcaa taagaacgca tagcagatca atgaaatcca
    11101 aaagaaaatt tttccaataa gtgggaagaa gttcccggca gttacagtga tgaccccaaa
    11161 tccaacgtaa atgataaacc agcttggata aattgcttca attgaaagat cagctttgat
    11221 aacatggtga taattgaaat aaaagacaag gatcacttgg aagataacag cgcacagcca
    11281 aatgaatttg acaattggtg caatactttc atatgaaaca aaataagtac aaataaccat
    11341 gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt ggatcatgca acgtttgttt
    11401 cgtgtgttcg aataagataa acaatttaac aaaaatgaat atcataagaa tcatagagat
    11461 accgccagtt agattgccta aagcaacatg gtgatagtct ttaaacagat ttccaaggga
    11521 agctaaccca aggatcaacc cgcagatcgg gatcggaata attttcaaaa agttcttcat
    11581 atataatatt ctccttttta tttaatagat tttagtacca agatgaacga atacattgta
    11641 aaatgaatag gaatgtatga ttacgagcaa gcagacagct gattgtcttc atctaagtta
    11701 ctagttggat cagcaactgt tttgttgtac atttctatta tagatggttt tttgtatatt
    11761 taaatcaaat agtttttata gtaattatca taaaaagtta tggaagtggt gccatgttta
    11821 agttattaac gacttttcga gcagtatatg aaacaagaaa tttttccaga gcagcagaag
    11881 tactgttttt atctcaacca gcagtctcga atcaaatcaa acagttggag cgggaattga
    11941 atatcgatct ttttcaacgt aacggaagaa aagagatgct tccaactaag caagccgact
    12001 tattatatga acggacactg gttttgttag aagaatggga agaaactcag caaaaattgc
    12061 tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc ttcccacacc tttgctgtct
    12121 atcttttgcc tcagttgatg aaacatctga tcaaaaggtt tcctaaagtg cattttgaga
    12181 tcgaaatcac gaattctcat gaatcattag aacaagtagc gaaacatgaa tccgattttg
    12241 gttttatcga aaagccatta gagacttccg gcgtacaaag atactctttg atggaagacc
    12301 agctagtcat cgctggggac ccaaaaagca aattgtggct tgtaagagaa gcaaactctg
    12361 gtgtttttca ttacacgcag cgttattttg aagagatgaa tatccaagat aagaagatga
    12421 tcgtgaaaag taacgatgta attgttgcgt tgctgaagga aggaatcggt cgctcgattt
    12481 tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc gttgaatttc catcgatatt
    12541 tttatttcat ccaacgaacc aatctggttt ctgatcagct gctagagatt gccgaagaga
    12601 tccttcgata caatgaaagc acagaaactt ctcacagcga ggagaatctc agctggtaga
    12661 taattgcatg tatcaaaaaa agttctatga tacactaaag ataagcagta ggcttttgta
    12721 ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt tttcccaaat gtctatggaa
    12781 agactttcca ttatctaact ggaccgctat aataatcagc agtatggttt ttaagaaaag
    12841 ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata atcgtattga ttatggcgtg
    12901 attttaccag tctttctgtt atgtttgatt ggtctgctct cattatatgt agcacttagc
    12961 catgatccga acaatccagc agtcttgaaa ggcgtaggga tgcaagttgt ctggtattta
    13021 gttggagcgg cagcaatcgt tgtcatcatg catttgaatt ccaaatggat ctggaaactg
    13081 actccttacc tttatggttt ggggttagtg gtaatgggac tcttgctcaa attctatgat
    13141 cagaatttag ctgcttcaac agggtctaaa aactggtttc gattcggtgc gtttactttc
    13201 cagccagcag agttgatgaa gattgcgtac atcttgatga tggcactgat cgtaactaaa
    13261 cataatacgc aagtaaaaga acggacgatg aaatcggact tttggctgat tgggaagctc
    13321 ttgatcgtga caataccggt tttagcattg atcatggccc aagatgactt tggaacgatg
    13381 cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt ctggaatctc ttggcgcatc
    13441 atcgcacctg tagtaatctt ggcagtagtc gttggagcag gaacgatctt cttggttacg
    13501 actgaaggcg gaagagactt gctttataaa gttggattca aatcttatca gtttgcacgt
    13561 attgattcat ggctggaccc tttccatgat acgtcaggaa tgagttatca gccggcccaa
    13621 ggacttttgg caattggtac cggtggcttg tttggtaaag gattcaacgt aagcaatatc
    13681 tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta tcggtgaaaa cttcggcttt
    13741 atcggtggtg cattcgtcat tttcttgtat tttattttaa tctatcgtat gattcgggtg
    13801 tgtttcgata cgaataatga attttacgcc tatatcgcaa gcggtttgat catgatgctg
    13861 ctgttccatg tctttgagaa tattggagca aatatcggat tacttccttt aacagggatc
    13921 ccattgcctt tcattagtca gggaggatcc tcgattttag ggaacatgat cggtatcggg
    13981 ttgatattat cgatgagata ccaaaatgaa gcacctgcaa gatcttcaag gaggtaagaa
    14041 aattgtacac cttttactgg tacccgaaat gttcaacatg caaaaaagcc aaagcgtggt
    14101 tagaccagca tggcgttagt tatcaaaccg ttgatatgat caaagaagta ccgaaggcct
    14161 caacgattga gacttggcta gaaacgagtc aagtaccaat ccgtcgcctg ttcaacacaa
    14221 gcgggatgaa atatcgggaa cttggactga aagatcaagt aggcgagttt acggcttcaa
    14281 aagcgagcca agtcttatcg acagatggta tgctgatcaa gcgaccactg atcttacgag
    14341 aggaccagct tgttgcaatt ggattcaatg aaaaaattta tgaaggagaa ctgatataaa
    14401 atggcaggca agcatttgaa gaaaaaggac aacctttgga tcttatttaa tggaaaagaa
    14461 tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg gagacatcac gtttgccaat
    14521 ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc tgatcgaagt tgaagcggaa
    14581 aaagcggtca atgaatttgc cagcccgcta acgggtacag tttcttctgt taatgataaa
    14641 atcggtgaag atatcaatat cttgaatgat tcagatgaaa tgaatgcgtg gatcttaagt
    14701 ttcaaagatg ttgatccaaa agaatttgac gaattataaa atcagaaaat cacttgaaaa
    14761 aagttgacac tgattcttcc agttgctata atcataagca atcctatatg aaacgagaaa
    14821 gctttgaaaa gaagagtaca tgtcacggac gtttgaagag agccctgttt gctgaaaatg
    14881 ggtaacgaaa atgagatgga agatggtctt agagcagagt aagcgagcgt aagcaaactt
    14941 actacgggag tgcccgttac agcgcgacag tatacggaaa gaagctaagt ccaagtactg
    15001 gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg taacacggaa gttcaagctt
    15061 tcgtcctttt gcttaaggca agagggcgaa ggcttttttg tttgaaaaat cataagagaa
    15121 agatgtgaat ggcatgtcat taatccaact aacgaatgtc aaaaagacat tcaatgggaa
    15181 aaacggaaac atccaagccg tcaatgacgt ttcactaaac gttgaaaaag gggatattta
    15241 tggaatcgtc ggctattctg gcgcaggaaa aagtacatta gtccgtctcc tgaatggact
    15301 tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa gatattacaa aattaaaaaa
    15361 caaagaatta cgtacattcc gaaagaaaat cggtatgatc ttccagcatt tcaacctgtt
    15421 atggtcaaga actgtacttg aaaatatcca gctgccgctg gaattagctg gcgtaccaaa
    15481 gagcaaacgt aaagaacggg cagaagaact gttgcgttta gtcgggttag aaggacgcgg
    15541 acaagcatat ccttcacaac tttccggcgg acagaaacaa cgcgtcggta tcgcacgagc
    15601 attggcaaat gatcctgaga tcctgctttg tgatgaggct actagcgcat tggacccgca
    15661 aacaacagaa gaagtattag acttattgtt agcgatcaat aagaaattga atttgaccat
    15721 cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc tgcaacaaag tcgctgtgat
    15781 ggagttgggg aaagtcgtag aagaaggaga cgtattgact gtcttcagac aaccgaaaaa
    15841 agaagtaacc aaacgctttg tacaacaaga catcgaacca gaagctgatt cgacagaact
    15901 attagctgaa ctcatcaaag aaaatccaag cggccgtttg gcaactttga cctttagcga
    15961 aagaaatgcc aacgagccag tcatttccca agcgattcga cgctataaca tcgatgtcaa
    16021 cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc tttggttcat tgactgtctt
    16081 gatgactggt gaaaccaaag aattagatca agcagaagtg ttcatcaaag aacaaggagt
    16141 aggaattgag gtgatccatc gtggataaaa gcttttcaga aacatatcta gattttaaac
    16201 aaatcaaccc ggaaacgatg aatcaggcta tccttgatac actatatatg acattaatct
    16261 caatggtctt agtgacgatc atcggattag ttttgggatt gatcctttac tctattggac
    16321 gcaaaaacaa accgtatgct cgaatcattt atggaatcgt atcgatcatc agtaatattt
    16381 tccggtcaac gccatttatg atcctgatgg ttttgatcat tccatttact aaagcaatcg
    16441 taggcactat gctaggtgcg caagcagcca ttccagctct tgttttatcc gcagcaccat
    16501 tttatgcccg tctagtagag attgcttttc gtgaagtcag ccctggtgta ctggaagcag
    16561 cagatgcaat gggagcaagt tactgggaaa tcatctggaa ggtcttgata cctgaaagta
    16621 ttcccgcatt gatttctgga ttaacagtta caacgatctc gatgatcgga tttactgcga
    16681 tggccggcgc gatcggcgca ggtggacttg gaggtcttgc ttggcaggaa ggctaccagc
    16741 gtggtaatct aacagtgaca ttcgttgcga ctttaattat tttactaatc gtatttatcg
    16801 ttcaaggaat cggtgatttc ttaactaaga aaacagacaa aagataatag gatatagggg
    16861 gatgtaggat atgaaaaaga aaatttttgg atttgcagca gcgcttttgc tgactgtagg
    16921 attagcagca tgcggtaaca acagcgacag caaagattca gcaaataaag cagacgacac
    16981 gacattgaaa gtcggcgctt caccgacacc gcacgcagag attttggaac atgtgaaacc
    17041 attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa ttcgatgact atgtattacc
    17101 gaaccaatct ttggaagaag gcgacattga tgccaattac ttccagcata ttccttattt
    17161 gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat gccggtgctg tccacatcga
    17221 accaatgggc ttgtactcta aaaaagtcaa agatgtttcc gaattaaaag atggtgcaac
    17281 aatcattact tcaaattctg aatcagactg gggacgcatc atcacaattc tacaagatgc
    17341 tggcttagtc aaagtcaaag atggagtaga tctggaaaca gcaacattcg atgatattga
    17401 agaaaaccct aagaacttga agttcaacca tacgatcgac ccatcacttt tagcttctac
    17461 gtaccaaaat gatgaaggtg acctagttgc aatcaacgca aacttcgctt acggcgcagg
    17521 gttgaatcca ttagaagatg ccgtattgct tgaaaaggac aactctcctt acgtgaatat
    17581 tattgcgact cgtaaaggtg atgaagacag tgacaaaatc aaaaaactga ttgaagtgct
    17641 tcaccgcaaa gaagtccaag aatggatcac agacaaatgg ggcggctctg ttaaaccagt
    17701 aaaggctgat gcaaaataag gtatggaaca aaaaagatga gaaataagtc tgtaatggct
    17761 tattt
//
LOCUS       DACDKL010000040        17626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDKL010000040 DACDKL010000000
VERSION     DACDKL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.040"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..1824
                     /locus_tag="IV329_001055"
     CDS             139..1824
                     /locus_tag="IV329_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8940255.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1838..3121
                     /locus_tag="IV329_001056"
     CDS             1838..3121
                     /locus_tag="IV329_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8940256.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3191..4060
                     /locus_tag="IV329_001057"
     CDS             3191..4060
                     /locus_tag="IV329_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8940257.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4057..4893
                     /locus_tag="IV329_001058"
     CDS             4057..4893
                     /locus_tag="IV329_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8940258.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4919..5986
                     /locus_tag="IV329_001059"
     CDS             4919..5986
                     /locus_tag="IV329_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8940259.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5983..6810
                     /locus_tag="IV329_001060"
     CDS             5983..6810
                     /locus_tag="IV329_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8940260.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6855..7880
                     /locus_tag="IV329_001061"
     CDS             6855..7880
                     /locus_tag="IV329_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8940261.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7877..10060
                     /locus_tag="IV329_001062"
     CDS             7877..10060
                     /locus_tag="IV329_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8940262.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10060..11190
                     /locus_tag="IV329_001063"
     CDS             10060..11190
                     /locus_tag="IV329_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8940263.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11187..11864
                     /gene="pgmB"
                     /locus_tag="IV329_001064"
     CDS             11187..11864
                     /gene="pgmB"
                     /locus_tag="IV329_001064"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8940264.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11948..12475
                     /gene="msrA"
                     /locus_tag="IV329_001065"
     CDS             11948..12475
                     /gene="msrA"
                     /locus_tag="IV329_001065"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8940265.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12676..13362)
                     /locus_tag="IV329_001066"
     CDS             complement(12676..13362)
                     /locus_tag="IV329_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8940266.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13442..14980
                     /locus_tag="IV329_001067"
     CDS             13442..14980
                     /locus_tag="IV329_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8940267.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15100..15567
                     /locus_tag="IV329_001068"
     CDS             15100..15567
                     /locus_tag="IV329_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8940268.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16086..16529
                     /gene="rplM"
                     /locus_tag="IV329_001069"
     CDS             16086..16529
                     /gene="rplM"
                     /locus_tag="IV329_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8940269.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16545..16937
                     /gene="rpsI"
                     /locus_tag="IV329_001070"
     CDS             16545..16937
                     /gene="rpsI"
                     /locus_tag="IV329_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8940270.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(17025..17573)
                     /locus_tag="IV329_001071"
     CDS             complement(17025..17573)
                     /locus_tag="IV329_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8940271.1"
                     /translation="MITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTK
                     GRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHAD
                     YLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYV
                     NTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         5745 a         3231 c         3789 g         4861 t
ORIGIN      
        1 taattattga tacataagct ttcttatttg tatttttgtg tatatcaaaa ttatttaaaa
       61 aaatgctatt gacagtaagc gttttccata atatgatcaa atcgttggga acgctcccaa
      121 aaaagaaagg ggaatccgat gtatacaaaa cttttacaag cgatttatcg cgactcagat
      181 ttgtctgaga aaaccgcctc tatgattgaa caaaaagtca gtgtttatca gcagaagaaa
      241 ggccaggcag gcaaactgca ttgggacgaa gaagatattt tcttgattac ttatggggat
      301 caattttttg aagagcagca accaactttg cgtacattta agaaatttta tcaaaagtat
      361 ttggcagata catttagagt tgtacatttc ttgccatttt tcccatatac atctgatgat
      421 ggtttctccg ttgtaaatta tcaggcaatc aacgaccgtt tgggcacatg ggaagatata
      481 gaagaaatga gaagcgaaac acggttaatg ttcgactttg tctgcaatca tatgtctgcc
      541 gaaagtactt ggtttcagga atatttaaac ttgaatgata cgtacaaaga tttttttatt
      601 gatttgccac tggatacaga tacaagcagc gtgactagac caaggaccag cccgttactt
      661 acaacgttca ttgacaaaaa cgggaaagaa aaacacgtat ggacgacttt tagcgaagat
      721 caagtagact tgaattttaa aaatccagaa gttttattga aaatgatcga tattcttttg
      781 ttttacttag aaaaaggcgc ttcatttatt cgcttggacg ctgtgggatt tttatggaaa
      841 gaactaggaa cttcttgtat acacctgcca gaaacgcata aaatcatcca attattccgt
      901 gcaatcgctg atgaagcagc tccaggaacg gtattgatca ctgagacaaa tgtgccacac
      961 aaagataata tcagttattt tgggaatggc gagaatgaag cacagatggt ctatcagttt
     1021 tcgctaccac ctttgaccct tcaagcgatt cgttcgggta atacaagtaa gttgaaagaa
     1081 tggttaaaaa ccatcgattt cgcatcgaaa aaaacaactt tttttaattt tttagcctcc
     1141 catgacggta tcggattgaa tccggtacga ggtattctgc cggaaaaaac gatccttgaa
     1201 ttagtggaac agctgaagaa agagggtgcc cgagtaaact acaagaaaaa cccagatgga
     1261 acgaccagtc catatgagat aaacgctacg tatctagatg cgttaagttc tccaacagat
     1321 acgacggaat ggagaagcaa acgcttttta gctgctcatt catttttggt aacgttacca
     1381 ggcgtgccgg caatttatat tcaaagtgtg ctgggaagtc gaaatgatta caaaggcgta
     1441 gaaaaaacag gagagaatcg aaaaatcaat cggcaaaaat ttttgttagc agatattgag
     1501 aaagagttgc agacagatcc attaagaaca gcagtatatg gtgaattgac ccagatgcta
     1561 tctattcgaa aaaaagaaaa agccttccat ccgaatagtt tgatttactt actggactca
     1621 gcagattctt ttatcagttt tattcgcgaa gcgaaggaag agaaagtctt agttatccat
     1681 aatttttcca atcaatcagc aggttatctt ttaccaaaag gagattacct agacctgttt
     1741 tctggacaaa ttatccattg cagtcaagaa cgaaagttga tattccagcc gtatgaatat
     1801 atgtggctga aaataataaa ataagaggtg gaaaaaaatg aaaaaaatta ttgttactac
     1861 ctgtctgcta gctactggat ttcttcttac tgcttgtggt gaaagtcaaa gctctaacga
     1921 atcacaacag acaacgattc aattcatgca ctcttctgtc gaacaggaac gattaagtgt
     1981 gatcaatgaa ctggttgctg attttgaaaa agaaaatcca gacatcaaaa tcaagcaagt
     2041 gccggtagaa gaatcagcgt tcaatacaaa aatcgttacg ttagctcgtt ccggtaaatt
     2101 accagaagta atggaagtca gtcaggattt tgctaaagtg atggataaag atgaactgat
     2161 tgatcagacg gcagtccaaa acgttatcga ggaagctgga gaagataatt attatgacgg
     2221 agccaaaaac cttgttcgtt cagaagatgg aaaaagctat attgctgcac caattagcgg
     2281 ctgggttcaa ggtatttggt atgacaaaga aaaattatca gaagctggtt tttcagaacc
     2341 agaaaattgg gatgatattt taaaaattgc tcaacatttc acagataaag agaataaaca
     2401 atatggaatt gctatgccta ctgctgaagg aacaatgagt gagcaagcgt tctctcaatt
     2461 tgctttgtca aatggagcaa atgttttgga tgatgaaggc gaggtaacga tcgatacgga
     2521 aaagatgaga gaagcattgt cgttttatca aaacttatcc caatacacga tgccaggctc
     2581 taatgatgtg acagaaatca aagatgcttt catgaatgga acagcaccaa tggcaatcta
     2641 ttcaacttac attttgcctt ctgtttatga agcggggaac tcagaaaata tcggttttgc
     2701 tatcccaaca caaaaagatc aagctgtcta tggcacagta tctggattga cgatttcttc
     2761 tggtctagac gagaaacaaa aagaggctgc tgaagcattc acagctttct tatctcaacc
     2821 gaaaaacatg gaaaagtggg tcttgatgtc tcctggtggt gctcaaccag tgaataaaca
     2881 agtagtcgaa ctggatggtt acaaagaaaa tgaagtcatc aagtcttttg gcgagctgcc
     2941 atctgagatt gctagtgctt ttgatgaagt acaagtcttt ggactagtcg gggaaaagaa
     3001 tttcacgaaa atgggagata tcacaagctc aggtgtgatc ggaaaagcag tgaaccaagt
     3061 aactgtcgga aacgaagatg tcgatcaagc tttagctgac gcacaaaaaa atacgaaata
     3121 acaagaagtt ctgccaatgg tgttcgaaaa aagtagatag ctattggcag attatcaggg
     3181 ggaggtttca atgaatcgtc caaggaaaca ttctgactta aaattagcca tgggactttt
     3241 agcaccaagc atgcttttat tagctgtttt agtgctgatt ccaatgcttt ccaatattca
     3301 gatcagtttt ttcgatatgc cgctgaatcc taactcagac gccgttttta tcggattaag
     3361 caattatctt gaaattttaa gtgatagtga attttatcgt tctttaggtt taacagttgc
     3421 ttacacattc ttagttgtag caggcagtac agctttagga ttagccgtag ctattttgct
     3481 taaccgcaag ttcagattta ggaaaacggt gcgttcactg attttattaa gttatgttac
     3541 gccatctatt tctttgattt ttgcttggaa atatatgttc aataatagtt atggaatcgt
     3601 caatgatctg ttagtagata ggttgcagct tttcaacgaa gcaccgctgt ggttcgacca
     3661 gccagttgct agcttcttta tggtcgtttt gtttgctatc tggagatact atccgtatgc
     3721 gtttatttca tttttggcta ttttgcaaac agtcgacaaa acgctttatg aagcagctga
     3781 aatggacggt gcaaatgctt ggcagaagtt caagatcgtg acactgccgg caatcaaacc
     3841 agtactagca actgtgattg ttttacgatc tatctggatg ttctatatgt ttacagatgt
     3901 ctatctactg acgaataaag tcaatatctt aggcgtttat ttgtatcaga cagcctttgc
     3961 gttcaatgat cttggaaaag cagcagctat atcgattcta ttgttcatca ttattgcagc
     4021 ggtcgtcttg ctctcacgaa agaaggtgga cttgaaatga aaaataaaaa gagcaggaaa
     4081 atcttatttt acgcgacgat tttgttgcta ttagctgtca atttgtttcc atttttgatc
     4141 atgctgatga catcattcaa ggattcacaa gaagcaattg caacgacaca aactattttt
     4201 ccgaaagaat ggacttttga acattatcag gatattttca atgctgagat ttttccattc
     4261 atcatgtatt tcaaaaatag tttagtgatc tccttgatag cctccgtaac tgcagtagtg
     4321 attgggatca gcggggcata cgctttgtca aaactacgct tcaaagctcg ctcgaaaatc
     4381 aatgctagct tttacaccgt ttatatgttt tccggtattt tgatcgttgt tccattattt
     4441 aaaatcattt ccggtctagg tttgtatgat accaatacag cactgattat cacgatggtc
     4501 gttcaaacac ttcctactgc gatattcatg ctaaaaagtt attttgacac gattccgact
     4561 gatattgaag aagcagcaat gatcgatggt cttagccgga tccagatcat tattcggatc
     4621 attataccat tggctatttc tggaatcatt tctgtcttcg tttattgctt tatggtttcg
     4681 tggaatgatt atttatttgc ttcgattttc ctgtcgacct ccgaaaaatt tactttacca
     4741 atcggtttga acacattatt cagtacacca gactacattt ggggacgaat gatggccgct
     4801 tcattggtga ccgctttacc agtcatcatt atgtatgcgt tatctgaacg atttattaaa
     4861 ggtgatttag tcgaaggcgg cgtaaaagga taaaaagaaa agaaagagga aagaaaagat
     4921 gaaaaaatta gttgcattag aaccaagaat agccggattc aaagattacg aagaccgtga
     4981 gatcgaaaag aacgaagtac gtatccgagt aattttcgct actccaaaac caggaacaga
     5041 aattgtcgac tttagaggat taagtccatt tatcgatgaa gaattttcag aagaatggcg
     5101 cctattcttg ccaagagaag aaggaagcca acgcggtgtg gtatttggcg aatttcaatt
     5161 aggaaatatg attgtcggta cgatcgaaga aacgggcagt ttggtgacag agtatcaaaa
     5221 aggagagtta gtctgcactt atggacccat tcgtgaaacg ctgattacaa acggtgttga
     5281 taattataaa ctacgtaaac ttgatcggtt ggatcaatgg aaaaatgcag tatgttacga
     5341 tcctgctcaa ttcgctttag gtggtatccg ggaagcacat gttcgagcag gtgatgctgt
     5401 agcaatcttc ggtttaggcg ctatcgggca aattgcgatt caattagcta aaaaagcagg
     5461 tgctactacc atcgttggga tcgatcccat agcgcagcgt agagaattag cagaaaaaca
     5521 cggtgccgat tacacgctag atcctatgaa agcagatgtc ggttttcgtc taaaggaatt
     5581 gacagataaa cgtggagtag attcagttat tgaaacgagt ggcagtgctg ccgcattaca
     5641 agcttcttta atagggatcg gctatggcgg gatcatttct tatgttgcct ttgctaaacc
     5701 ttttccagct gggtttaatc ttggtcgcga agcacatttc aataatgcaa aaatcgtctt
     5761 tgctcgagca tctagtgaac caaacccaga ttacccaaga tggaaccgta aacgaatcga
     5821 ggacacatgt tgggaattat tgatgaacgg ttacttggat tgtgaagaaa tcattgatcc
     5881 agttgtgcca tttgcagaaa gcgcagaagc ttacatgcgt tatgtagacc agcacccaga
     5941 agaaagtatc aaaatgggcg ttcaagtccg aggaggagaa caatgaaatt cggtacgcaa
     6001 gatcaagcat ttttcccaag agacatttta gaaaaattta gatttatcaa agactgtgga
     6061 tttgaagaat acgaaatcga cggtaagttg ttaatagaaa atatcgacac agtcaaacag
     6121 gcaatcaaag aaacagggat cccagtagtg acagcttgta acggctacga tgggtggatc
     6181 ggtgacttta tcgaagaacg gcgtcttaat ggtgtacgtc aaattgctga gattttgcgt
     6241 gcactaagcg aggttggagg caaaggaatc gttatcccag cagcttgggg aatgttcact
     6301 tatcgattac cgccgatggt ttcaccccga agcaaagaag gagactataa agcagtcagc
     6361 gaatcacttc gttatttaga taaagtagcc gaagaaacag gtacggttat ttatttggag
     6421 cctttgaacc gataccaaga ccatatgatc aatttgttag cggatgcgcg tcattacatt
     6481 gactctaatc aattgaaaca tgtgcagatc atcgctgatt tttatcatat gaatattgaa
     6541 gaagatgata tttcaaaagc attgactacg caaaaagagt atgtgggtca tatccattta
     6601 gcagataatc atcgctacca accaggatct ggcgcaattg attttactcg ccattttcaa
     6661 acgctacttc aaaatgagta tgaaggttat atggtatatg aatgtcgttt gcgaggagaa
     6721 aatgaagtga aaatttatca ggaatcattg caatacttga aaaactgtct ggaaacagcg
     6781 cgtcatttaa atgatttaga gaaagaatag agaaagaata acaataagta aataagcaaa
     6841 ggagagagaa ataaatgaag aaagtagcaa tcataggcgc tggacaagta gcagaaaaag
     6901 tacatgctgc ttattatcaa aaaagatcag atgtcttaga attggccgct gtggttgatc
     6961 cagtcagcca gcgagcagaa gacttttgcc gacgtacagg attttctcat gcgtatacat
     7021 cagttacaca aatgctgaat gagatttctc ccgatatcgt gagtgtctgt acacccaatc
     7081 gctttcactt cgaacatgta ttggctgccc tcgaagctaa tgcttcagtg atgtgcgaga
     7141 aaccaccagc tatgacagct gatcaagcaa gaaagatggc agagacagcc gataaaaacg
     7201 ggcagatttt ggcttatgga ttccagcatc gtttttctga cgaagcaaca atgatcaaga
     7261 atcatcttga tgttctcggg gatatttatt acgtagaagc gaatgcgctt cgtcggtctg
     7321 gtgttccagg ctggggaacg tttatcgaca aagaacttca aggaggcggg cctttgatcg
     7381 attatggcat tcacatgctt gatacagcga tgtatattac tggatttcct aaagtaaaaa
     7441 gcgttcgggc gtacagcttc caaaaaattg gtacgaaaaa aaattcaggg aaattcggga
     7501 aatgggatcc aagcaaattc acagtagaag atgcaatttt tggcacactg gaattagaaa
     7561 acggcaagat catccaactg aatacttcgt ttgctttgaa tatcagagaa gacaaactga
     7621 atgtggcttt ttgtggcgat caggcaggtg ctaatctgta tccaggcgaa ctattcgaag
     7681 atgtagatgg aaagctggtt tccttacaaa agaccacgcc agttaatgac ccacatgaac
     7741 taagtatggc tcattttgtg aatcgggcgc tcggtgacca gactcagatg attgcagatg
     7801 gctggcaagg atatgtcatc cagaatctgg tggaaaattt atatcgttcg gcagaaacag
     7861 gagaagttat taccttatga aagtagaaga aaaacagttt gatttagagt atatcaataa
     7921 gtatgcgagt cagatgacca ttggaaatgg attttttggt atcagaggaa gccaggagga
     7981 agactatcgg aatcaagtaa ggggaatgtt tgcagctggt gtatataacc gacctttagg
     8041 agcagaatca gccgagttag tgaatctccc agatgtcatc agatgggaaa taaaaatcga
     8101 cggtgaacat ttttctcttg agacgatgaa agttcttgca tacgagcggg cactagattt
     8161 agaaatggga gagcttaaac gctatgcgct cattgaaact gctcaaggaa aaaaagtgga
     8221 agtcattact tgtcgtgttg ctgatcaaaa taatctccat caggcagccc tgaaagtggt
     8281 catcaagccg ttgaccgaag actgccaagt agatgtgaaa acgggtatcg atggacagca
     8341 aaccaatttt ggtacacagc agttggttga aaaagaactg cgagtatttg gtgacgattt
     8401 gatcagtgca tcctaccaga caacagaaag taaaattgat attgggattg cagctaaatt
     8461 caataaagca ggtgtattcc aagcaaaaaa tcgccggatc accgcttcct tttcagaaaa
     8521 gatcagaaaa aatacgtcct ttacgctgga gaagatcatc gtgctctcta cctctcttca
     8581 gcaaacagac ccgtttaaga aaagtattgc ccaagcccag aaaagcgtta gttatacaga
     8641 aattttagag cagtcgatgt cttattggac agatttttgg catcagcatc ggataacgat
     8701 ccaaggcgat tcttctttcg atcagttagc gatggacttc gctgcttacc atttacagat
     8761 tatgactccc cgcaatgatc caagtgttag tgtaggggcg aagggactaa ctggcgaagg
     8821 ttataaaggg catgttttct gggatacgga aatttttatc ctgcctttct ttttatatca
     8881 gtttcctgaa gtagcaaaga atttgctgct gtaccgttat gatcgattag aaggcgcacg
     8941 gcaaaaagca agagattatc actatcaagg tgcacttttc ccatgggaaa gcgcacacac
     9001 tggtcaggaa gagacaccca aatttgctgc attgaatatc aaaaccggaa caagacaaga
     9061 agttgcttca gctgccgcag aacatcatat cagtgcagac cttgcttatg ccgttcaaga
     9121 gtatgtccaa gcaacagagg atcaatcgtt tttagaaaca aagggaaaaa cgctcattca
     9181 tgaaacagca gctttttggc tgagcagagc tgtaaaagtc gatgatcatt tagagataca
     9241 cagagtcatt ggtccagatg aatacacaga atatatcgac aataatgcat atacgaacta
     9301 tctggcgcat tataatgtgg ccttagctgt taaatatcaa gctggcgatc cgcttttttt
     9361 gaaagaatgt caaaattttt tagataagtt atatttgcca gtgcctaaca aagaacagct
     9421 gattccgcaa gatgacacgt atctcagtaa gccagttatt gatttgacca cttacaaaga
     9481 acgtcctggc agccagtcta ttttacttga ttatgcccgt agcgaagtaa atgaactcca
     9541 agtcagcaag caagcagatc ttgtcatgct gttatactta ttacctgagt atttttctca
     9601 accaatcgtt tctaagaatt tgcattacta tgaaaacaaa acaatccacg actcatcgtt
     9661 gagtaaagcg attcacgcca tagaggcttg ccgtatccaa gaaatggaat gggcgtatca
     9721 tttattccaa gaagcgtgcc tgattgattt aggaccagat ccgcattcat cagatgacgg
     9781 aattcatgct gcttcattag gagcactatg gctagcagtt atatttggtt tttcaggtat
     9841 tacagtaacg aatggaactc tttctatttc tccaagactt ccttcagcat gggaaaaaat
     9901 ttcttttcct tttatttgga aaaatcacag gttgctattt gttttgacaa aaaaacaaat
     9961 agtaataaaa aaagagacga aagaaacatt acctattttt gtcaatggag accaatacca
    10021 gctggaaaaa gaactgatct tggaacggag gggaaaataa tgcgtattct gattgcgcca
    10081 gattctttca aagaaagtct gacagcctta gaggcagcac aagccattca atcaggtttt
    10141 caagaagttt ttgttcaggc tgaatatgat ctgctaccta taggcgatgg aggagaagga
    10201 acggtttcat cattagcaga cagtttaagg ttgaagcggc aaacagcagt cgttactaat
    10261 gcatttggca aaaacaaaga aatccagtac gcttcagacg gtcaaactgc gatttttgaa
    10321 atggcagaaa tcgtcggatt gaaagatgtc ccactaaacc aacaacagcc acttaggtta
    10381 tccacgcgag gagtaggaga actgatacga caattgttgc aacaaggtgt tcgacacatc
    10441 atcatcggcg ttggcggaag tagtaccaac gatggcggca tcggcatggc agaaggtcta
    10501 ggttatgctt tttacgatca agataatcaa aaagtagaag cactgggaga aaatctaggt
    10561 aaaattgcta cagttgataa aacgaacgtg cctattgagc tgattaaagc caaaatcact
    10621 gtcattactg atgttactaa tcttttatgc ggaaaagaag gcgcttcttt tgttttcgga
    10681 ccacaaaaag gcttgaaaga aaatgaatta gcagtggctg accaagccat gcatgatttt
    10741 tatatgagat ttgcccctca agtaatcgag gcatcgggca gtagtgctgg tggtggaatg
    10801 ggtgcaggac tgttagcatt tgcttcaggg aaaatagtct ctggcatcga ctacgtattg
    10861 gatttactgg agttcgataa acgggtacag caagcagacc tcgtaatcgt aggcgaagga
    10921 cgtctagatg gacaaagtct aaaaggaaaa gcgccagttg ggattgctag aagagtacca
    10981 gagggcgttc ctgtgatagc tatttgcgga agtgttggta aaggaagcga aaaagcagca
    11041 gatgttggca tttcggccgt ttttccgatc cttcctgcag ctgtgccttt acaagaagct
    11101 ttacagcaag ccactgataa tcttcatcgc acagctaaaa acatcgcatc tttacttcaa
    11161 gtcgttcatt ttcaaggagg taatcgatga aaatcaaagc agcactattc gatttagatg
    11221 gcgttcttgt agacacagct cgttatcatt acgaagcgtg gctggtttta gctaaccagc
    11281 tgtccattcc attcactgag aaagaaaacg agcaactgaa aggaatatcc agaaccgagt
    11341 cactagagcg attgctgtca tttggaaaaa tggaacagaa attctcagaa aaagagaaat
    11401 cagcctttgc tgagcaaaaa aacaatctat atcttcaagc gattcaaaaa atggatgaaa
    11461 catccgtttt gcctggtgca atagctgttc ttgagtactt gaaaaaaaca aacatcaaga
    11521 tcggattggg atcagcaagc aaaaatgcac gcctcatttt agaaaaaacg aatttgacaa
    11581 gttattttga tgtccttatt gacggcacgc aagtatcaaa agcaaaacct gatccagaag
    11641 tttttctcaa aggagcacag caattgaatg taccgccaaa tgcatgcttg gtcattgaag
    11701 attcagaagc tggttgtcaa gcagctttgg ctggtaacat gcatgtattg ggaataggtg
    11761 aaaacataaa tcttccttct gctgaatatg tgattcctga tttaaccgtt tttgatcaag
    11821 tacgttcgtt ttggcattta tcagaagccg tatcacgtat ataatagttt ccgttttttc
    11881 tgtcacaagc aaaacgttat cctccttttg gaaatcaagg tatgatgaga aaaaaggggg
    11941 attttatatg acaaaaaaag cagttttcgc aggcggatgc ttttggtgta tggtcaaacc
    12001 atttgaagag cagccaggga tcatttctgt gacatccggt tatactggcg ggcatgtacc
    12061 aaatccaact tatgaacaag taacaacagg cacaacaggg catacagaag cagtagagat
    12121 cgaatatgat ccagaagtta tttcttatga tcagttggtg gaaatctatt ggcagcaaac
    12181 cgatccgact gacgcattag gacagttcgc tgatcgcggt gattcttatc gtccagtgat
    12241 ctactataat gatgaagaag aacgaaatat tgcagagcgt tcaaaacaag cattgcaaga
    12301 cagcgggcga ttcgataagc caatcgtgac aaaaatcgaa ccaagaagcg aattttatcc
    12361 tgcagaagat taccatcagg attattacaa gaaaaacaag ctccattaca atctttatcg
    12421 agaaggttct ggacgtgcag gtttccttcg tcgacattgg ggcgtcaaga aataagaagc
    12481 cgaaaaagct acttatggaa caccgtatat cagaggttgt aaaaccagca ttttgagcta
    12541 atactgaggg tcagctgatt gaacaccatt tagtggagag gttgtgaacc tgtcgcctag
    12601 ctccaagtca caaggatttg gtaagagaac actatatatc agaggttgtg acaagtcgtt
    12661 tgtcacaacc ttttttcaat acctaagatg aaggacaatc agcaacgcac tgctcaaaaa
    12721 aggaatgaaa ggcagctttt ccaacttttt tttatgacag ataaagtaaa cagaaaaaac
    12781 ggatattcca aacagactag caagaaacaa gatctgaagc aactcttcta gagacaagcc
    12841 accactccaa gaaagcatca acagagtatc gccagctccg agataggatt gaccatagcg
    12901 aaggcacaca ccgcctacga taaacgttaa cagcacactt tgccattgga agacgccggc
    12961 ctgcaaccaa aaaatccata aaattgccca agaacagaat agcgtgtgag aatggaccat
    13021 gaagtaaaat aaatccatca acgaaaatag caaagccgaa agcatccata tgaacgtcga
    13081 aaaatttctt gcggaagaaa aagtaaagaa ccaggcaaaa agaccaccac ataaggcttc
    13141 tgccaacaaa tagtagaagg gcaaccgaca cctgcaatag cggcagcgaa atcgttggac
    13201 gatgatcgag agaatcggaa tcaattcata ccaagaaagc gaacgacagc atttgacaca
    13261 atgagatcta ggataaataa tagagtgacc taacggaatc cgctgggcgg ttaaacataa
    13321 aaatgatccg atacagcaac caatgataaa ataaagtaac acgccatctc ccccttttca
    13381 aaaccaagat acgcaagctt aaactaaatt tttgtacata cataaaattt gaggtgaaaa
    13441 aatgaacgtt tcaatcggat tagatatcgg aaccacacaa accaaagcag ttgctttcac
    13501 agatgaagcc caagaagcag catccgctta ttttcgctac cctttgatcc aagaaacaca
    13561 agggatggca gaacaagatc aggaattgat ctttcaaggt gtctgcaccg ttataaaaga
    13621 agtaattaaa cagttgcctt cagacacaat cattcaattt gtctccttct cctctgcgat
    13681 gcatggcctg atattgttgg atgaagagaa aacaccgtta tctcgaatga tcacttgggc
    13741 agataaccga gcagcggacg aagcagaaaa actgaaatat accgtgcttg gcaaagaatt
    13801 ttatcggcag actgggacgc cgattcatcc aatgacacca ttgaacaaaa tcaagtggct
    13861 gcaaaacagc caacctgaat tattcaaaaa aactgcttat tttgtcggta tcaaagacta
    13921 tatcttttat agattgtttg gcgaactgat cagcgactac ggaacagctt caggaactgg
    13981 atattttgat atccatgaat tccaatgggc agaagaagtg cttgactact tgaacattcg
    14041 aatagagcaa ttgccgcaag tctttccttc cacctatcaa gtaacaggtc taccatcaaa
    14101 aacagcagca gaactaggtt tgccaaatga tatatcattt gttttgggag gggcagacgg
    14161 gccgctttct aatctagggt taggtgcttt tggtgatacg atcgccgctt tgaccgtagg
    14221 aacgagcgga gcacttcgtt ttattactac tcagcctcag ttccatccag aaatggagac
    14281 gttttgttat gttctggatc agaaacattg ggtagtggga ggagcgacta gcaatggtgc
    14341 tggcatcttt gattgggcag ggcatacatt tttacaggaa gtcgttcaat ctgctaaaga
    14401 aaaaggagaa aatccatatg atgcactgct ttcggctatc gcttttgtgc cagcgggtgc
    14461 caatggtctg atttttcatc cctatttact tggtgagcga gcaccgcttt gggacgcaga
    14521 tgcgtttggc agttttatcg gtttacagcg gcaacataca gaaaaagaaa tgatgaaggc
    14581 gattctagaa gggatctgtc tcaaccttta tcggatttta aaaggagttt gccaggaaaa
    14641 tcagctcaca gagatccgtg ctacaggcgg gtttgctcaa tctccgattt ttcgtcagat
    14701 gatggctgat gtactgggtt acccgcttgt tttccctact gtcacagaag catccgcatt
    14761 aggagctgtg attttaggct ggagaagcat cggaaagatc caaagtatca gtgaagcaca
    14821 ggaattgata gaattgaaag agcaagttgg agtacaagaa actgatcata aaaaatatca
    14881 gaaaatctat ccattatttg tttcgacaca aaagcattta gcacagactt atcagcaatt
    14941 tgctcaactg cgagaagaat tagctgattc tgaaaaatga ttggctttac ggataaaaaa
    15001 gcgaactgaa aatcgttctc aggtaaatga ttttgttgta cttttcattg aaagtctact
    15061 attattttaa gtgtaagaaa atgttaggag gcaagaaaga tgaaattcca acaaaccaaa
    15121 gaagtattaa atcaattagt tgcagatcta agccaaatgt ctgttgttgt tcaccaaaca
    15181 cattggtaca tgagaggacc aggtttcctt acattgcacc caatgatgga tgagttcatg
    15241 gatgatttga acgctcagtt agatgaagta tccgaacgct tgatcacatt agacggttca
    15301 ccttattcta cattgcgtga atttgcagat aacacaaaaa tccctgatga aattggccgc
    15361 tgggatcgta ctattccaga acgtttggaa acacttgttg caggctatcg ttatctagca
    15421 gatctttacc aaaaaggaat cgaagtttct ggagaagaag gcgacgatcc aacacaagat
    15481 atctttatcg aattcaaaac agccacagaa aaacgtatct ggatgatcca agctcatttg
    15541 ggcaaagcgc cagagatcga cgcgtaaaaa ctgttgttga acttgaccga aaaagaggtt
    15601 gtgaaatcag cgttttgacc tgagtattag agaaacaaac gtcaaaatag ttcctcatat
    15661 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt accaagaggt
    15721 tgtaagcctg ccgttcagct ccgagtcaca agaaacaatt ttaaaaaaca gcttcttatg
    15781 ttttatggat attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcaaaa
    15841 taaggggttg tgacaagact tttgtcacag cctctttttg tttttcattt tgacaggaaa
    15901 cgaccctttt atataaaaga aagaaaagaa gtaaagtttc atataaattt ttcaatcaag
    15961 cagaaaaacg ttgacattga ctagattagc gggtaatatg ttaaatggta ttgtttgccc
    16021 cacaaagagc cccggaaact cgaagtgtat gtcaaacatc agaacttaaa ttggaggaaa
    16081 ataacgtgcg tacaacatat atggccaaac caggcgaagt agaacgtaaa tggtatgtag
    16141 tagacgcaac tgatgttcca ttgggacgtc tttcaacagt tgttgcatct gtactacgtg
    16201 gtaaaaataa accaactttc acacctcatt tggatactgg cgatttcgtc atcgtcatca
    16261 atgcagacaa agtgaaatta acaggtaaaa aagcgactga caaaatttat taccgtcaca
    16321 gcaactaccc aggaggattg aaatcaatca ctgctggcga attgcgtgct aaaaattctc
    16381 gtcgtttgat cgaaacttct gtaaaaggta tgcttcctaa aaacacttta ggccgcaaac
    16441 aatttacaaa attgaacgta tacggtggag cagaacatcc acatgcagca caacaaccag
    16501 aagttttaga catcacaaac ttaatctaat aggagggaat ttcattggca caagttcaat
    16561 atatcggcac aggccgtcgt aaaaacgcag ttgctcgcgt acgtctagta ccaggaactg
    16621 gcaaaattac tgtaaataaa aaagatgttg aagaatacat tccacatgct gacttgcgtg
    16681 aagtaatcaa tcaacctttc gctgttactg aaacaaaagg cgcatatgac gtatttgtta
    16741 acgtaaacgg cggaggatac gctggacaat caggagctat ccgtcatggt atcgctcgtg
    16801 cattgctaga agttgatcct gacttccgtt tagctcttaa acgtgctggc ttacttactc
    16861 gtgacgcacg tatggtcgaa cgtaaaaaac caggtcttaa gaaagcacgt aaagcttcac
    16921 aattctcaaa acgttaatat cctttatata tcaacgtttc tggcactttt caattatttg
    16981 gaaagtgctt cttttgtttt taagaacaca tttaagaaca catatcaatc acacttattc
    17041 aatcctctct gaaaagcttc aaaaacagcc ttatcaacta tatctggtgt gttcacatat
    17101 atctttgttg tagaaatatc tgagtgtgtt aaagcatttg aaatttgatt catatctgct
    17161 ccaccttgtc tagctatagt tgcataagta tgacggagtt tgtgtggaga taaatgaact
    17221 aagtcaggat gccttctttt aattgaattg attcgatagt ttaggtaatc ggcatgaaca
    17281 ggacaattta cttctccaga tggtttagta taagtaaaga gatactgatc taacgtttgc
    17341 ttaattttta atttcaacaa ttgatcagct tgtaccgatt tccattcact tagcaatgtc
    17401 attacaactt ctggtacttg aatgacggta tcttttcgac cttttgtaaa cttttttctt
    17461 tgatatttat ctaaagtatg tttaagacgg accgtttgtt tttcaaaatc aatatatttc
    17521 cattgcaaag cgtaagtctc actttttcta tcaccaagat acagggtaat cataaaaagt
    17581 gtgtaatcat gaaaagtaag taaatgatta ttaaaatcat cattta
//
LOCUS       DACDKL010000041        17540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDKL010000041 DACDKL010000000
VERSION     DACDKL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.041"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..277
                     /gene="yaaA"
                     /locus_tag="IV329_001072"
     CDS             35..277
                     /gene="yaaA"
                     /locus_tag="IV329_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8940272.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            264..1388
                     /gene="recF"
                     /locus_tag="IV329_001073"
     CDS             264..1388
                     /gene="recF"
                     /locus_tag="IV329_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8940273.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1385..3331
                     /gene="gyrB"
                     /locus_tag="IV329_001074"
     CDS             1385..3331
                     /gene="gyrB"
                     /locus_tag="IV329_001074"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8940274.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3351..5822
                     /gene="gyrA"
                     /locus_tag="IV329_001075"
     CDS             3351..5822
                     /gene="gyrA"
                     /locus_tag="IV329_001075"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8940275.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6058..6357
                     /locus_tag="IV329_001076"
     CDS             6058..6357
                     /locus_tag="IV329_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8940276.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6407..6919
                     /gene="ssb"
                     /locus_tag="IV329_001077"
     CDS             6407..6919
                     /gene="ssb"
                     /locus_tag="IV329_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8940277.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6945..7181
                     /gene="rpsR"
                     /locus_tag="IV329_001078"
     CDS             6945..7181
                     /gene="rpsR"
                     /locus_tag="IV329_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8940278.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7324..9297
                     /locus_tag="IV329_001079"
     CDS             7324..9297
                     /locus_tag="IV329_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8940279.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9303..9755
                     /gene="rplI"
                     /locus_tag="IV329_001080"
     CDS             9303..9755
                     /gene="rplI"
                     /locus_tag="IV329_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8940280.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10029..11396
                     /gene="dnaB"
                     /locus_tag="IV329_001081"
     CDS             10029..11396
                     /gene="dnaB"
                     /locus_tag="IV329_001081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8940281.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12176..13468
                     /locus_tag="IV329_001082"
     CDS             12176..13468
                     /locus_tag="IV329_001082"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8940282.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14204..15067
                     /locus_tag="IV329_001083"
     CDS             14204..15067
                     /locus_tag="IV329_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8940283.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15196..16818)
                     /locus_tag="IV329_001084"
     CDS             complement(15196..16818)
                     /locus_tag="IV329_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8940284.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17067..>17540
                     /locus_tag="IV329_001085"
     CDS             17067..>17540
                     /locus_tag="IV329_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940285.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGF"
BASE COUNT         5722 a         3179 c         3750 g         4889 t
ORIGIN      
        1 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
       61 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
      121 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
      181 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
      241 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
      301 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
      361 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
      421 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
      481 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
      541 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
      601 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
      661 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
      721 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
      781 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
      841 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
      901 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
      961 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
     1021 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
     1081 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
     1141 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
     1201 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
     1261 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
     1321 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
     1381 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
     1441 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
     1501 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
     1561 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
     1621 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
     1681 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
     1741 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
     1801 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
     1861 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
     1921 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
     1981 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
     2041 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
     2101 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
     2161 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
     2221 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
     2281 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
     2341 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
     2401 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
     2461 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
     2521 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
     2581 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
     2641 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
     2701 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
     2761 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
     2821 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
     2881 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
     2941 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
     3001 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
     3061 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
     3121 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
     3181 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
     3241 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
     3301 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
     3361 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
     3421 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
     3481 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
     3541 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
     3601 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
     3661 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
     3721 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
     3781 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
     3841 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
     3901 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
     3961 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
     4021 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
     4081 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
     4141 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
     4201 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
     4261 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
     4321 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
     4381 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
     4441 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
     4501 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
     4561 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
     4621 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
     4681 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
     4741 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
     4801 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
     4861 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
     4921 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
     4981 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
     5041 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
     5101 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
     5161 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
     5221 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
     5281 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
     5341 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
     5401 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
     5461 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
     5521 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
     5581 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
     5641 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
     5701 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
     5761 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
     5821 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
     5881 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
     5941 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
     6001 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
     6061 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
     6121 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
     6181 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
     6241 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
     6301 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
     6361 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
     6421 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
     6481 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
     6541 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
     6601 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
     6661 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
     6721 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
     6781 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
     6841 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
     6901 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
     6961 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
     7021 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
     7081 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
     7141 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
     7201 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
     7261 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
     7321 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
     7381 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
     7441 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
     7501 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
     7561 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
     7621 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
     7681 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
     7741 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
     7801 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
     7861 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
     7921 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
     7981 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
     8041 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
     8101 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
     8161 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
     8221 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
     8281 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
     8341 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
     8401 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
     8461 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
     8521 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
     8581 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
     8641 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
     8701 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
     8761 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
     8821 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
     8881 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
     8941 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
     9001 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
     9061 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
     9121 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
     9181 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
     9241 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
     9301 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
     9361 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
     9421 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
     9481 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
     9541 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
     9601 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
     9661 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
     9721 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
     9781 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
     9841 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
     9901 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
     9961 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    10021 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    10081 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    10141 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    10201 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    10261 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    10321 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    10381 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    10441 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    10501 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    10561 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    10621 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    10681 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    10741 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    10801 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    10861 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    10921 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    10981 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    11041 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    11101 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    11161 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    11221 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    11281 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    11341 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    11401 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    11461 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    11521 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    11581 caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
    11641 gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag
    11701 tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt
    11761 aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    11821 acgcccaatc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
    11881 tgcaggacag ctttttcaca acctctgatt cacggtgttc caaaagtagc ttcttcgaaa
    11941 aataagtcga aatttcacaa aaatttgaga agcaattttc gtacattcct tcttatttct
    12001 tgaagctgac cacttctgtt acaacctctt ttttaataac cattctaaaa agataaagtt
    12061 cgttttttaa gcgtttttat aaaataaaaa taaaaatcat tcgattttat ttgaaatatg
    12121 tatctattat tgatacaatg gttcaggatt aaataatata gaacgaggtg ttcacatgtc
    12181 atctgtagta gtagtaggta cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt
    12241 tctcagtgaa aatgctgaag tgattgcacg gtaccaaggt ggcgacaatg cagggcacac
    12301 aattaagttt aacggtgtga cttataaact gcatttgatt ccttctggga ttttttataa
    12361 agacaagatc agtgtgatcg gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga
    12421 attagcttat ttacatgaaa aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc
    12481 gcacgtaatc ttgccttatc atatcaaatt ggaccaatta caagaagatg ctaagggcga
    12541 aaataagatt ggtacaacga ttaaaggaat cggacctgcc tatatggata aagcagctcg
    12601 tgtaggtatc cgtattgcag acttattaga taaagaaatt tttgaagaac gtcttcgttt
    12661 gaatctagat gacaaaaacc gtcaatttgt aaaaatgttt gattcagaac cacttgattt
    12721 tgacgatatt tttgaagaat attatgaata cggacaacaa atcaaacaat acgtcacaga
    12781 tacttccgtt atcttgaatg atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc
    12841 acaaggtgta atgctggata tcgaccaggg aacttatcct tttgttactt cttctaaccc
    12901 agtagccggt ggggtaacaa tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt
    12961 cggcgtctgc aaagcctata cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt
    13021 tgatgaaaca ggagaacaaa tccgcgaagt aggacgagaa tacggtacga caactggtcg
    13081 tccgcgtcgt gtaggctggt ttgattctgt agttatgcgc cattctaaac gagtttcagg
    13141 tattaccaac ctatctttaa actcgatcga tgtattaagt ggattggaaa cagtaaaaat
    13201 ttgtacggct tatgaattag atggcgaact aatctaccac tatccagcaa gcttgaaaga
    13261 gttgaaccgc tgcaaaccaa tctatgaaga attgccaggc tggtcagaag acattacagg
    13321 atgcaaaaca cttgcagaac tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga
    13381 gcttgttggt gtacgtatct caacattttc tgtcggacct gatagaacac aaacaaacat
    13441 cttagaaagc gtctgggcgc aaatctaagg ataaaaacga cgactgaatc aggacgttct
    13501 taccagagcg agtcgaagtg tttatctata cgataataat aacttgaaca ctgtttattc
    13561 agaataaggg actgtgacaa aaaccgtgtc acagtccctt tttccgaaca aacggtgttc
    13621 aaaagcttgc atctacggca ctaagcccaa tcagatgaaa aatatggagt gctattttta
    13681 tctgattgtt cttactgctt gatgcagagc agctttttca caacctctcc actaaacggt
    13741 gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt
    13801 tttgaaattg ttccttgtga ctcggagcta aacggcaggc tcacaacctc ttggtaaacg
    13861 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    13921 ttttccgttt ttttctctaa tactcaggtc aaaacgctga tttcacaacc tctgattcac
    13981 ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaat ttgagaagca
    14041 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctcttattt
    14101 tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag atttgctata
    14161 atagacgata agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac
    14221 agattcttgt tgtgatctgc cataccatgt attagcaaaa gaaaaagttg attttttatc
    14281 aatgaatctc caattgaatg atcaagagtt agtagatgac ctcggaaaga cattcaatta
    14341 tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt
    14401 tggccgatat ttagaatttt tccgttctta tgcggaaaaa cagattcctg ttttatatgt
    14461 ggctttttcc agtggattaa gcggctctta cagtagtgcg cttcaagcgg ttgaattgct
    14521 gaaggaagaa tatgaagacc ctgagatcta tgtttttgat acaaaagctg caagtattgg
    14581 ggaagggctt ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga
    14641 aaccctagag tggctggaaa gtaattattt gaaagtccat tcatgggtaa cagtagacga
    14701 tctgaaatat ctagaacgag gcggtaggat ttccaaagca agtgctactc ttggcagtct
    14761 attgagtgta aagcctatta ttacggtgga taaagaagga aggctgacga atgttgacaa
    14821 agttcgagga agaaacaagt cgatccagac aatcgtcaat gagacggtaa aaacaatcgt
    14881 tgcaccactt gatcaaatcg tttatgtggc ttacgctggg gatctagaga gcgcggagaa
    14941 ggcaaaacgg cttttggaag agcagataaa gatgaaagat gtgaagcttt atcccttagg
    15001 accaacgatc gccagtcaca caggctatgg ctgtatcgcg attttttcga tgggaatcag
    15061 tcgataaaag cagtaactca ttctgtaaaa aagcccagaa attgattgta acaaaaacag
    15121 cgactcttaa aagatagatg agcatcctat ctcttagagg tcgctgtttt tttatttttt
    15181 aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc
    15241 caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat
    15301 gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc
    15361 agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt
    15421 tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg
    15481 gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa
    15541 cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg
    15601 acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa
    15661 ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc
    15721 catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt
    15781 gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac
    15841 acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac
    15901 gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt
    15961 tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc
    16021 ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc
    16081 tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa
    16141 atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac
    16201 gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc
    16261 cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc
    16321 taataacact tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc
    16381 ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt
    16441 aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc
    16501 ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat
    16561 gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc
    16621 catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa
    16681 cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc
    16741 gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac
    16801 atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt
    16861 atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca
    16921 cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt
    16981 tattagtttg caacagcata atttttgacg ttatttataa gagggagtac tataaaactg
    17041 taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat
    17101 tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa
    17161 atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac
    17221 tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa
    17281 tcgggatcat cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg
    17341 cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta
    17401 ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata
    17461 ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat
    17521 taagcttttt agttggattc
//
LOCUS       DACDKL010000042        17537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDKL010000042 DACDKL010000000
VERSION     DACDKL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.042"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..263
                     /locus_tag="IV329_001086"
     CDS             33..263
                     /locus_tag="IV329_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8940286.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            360..1661
                     /gene="murA"
                     /locus_tag="IV329_001087"
     CDS             360..1661
                     /gene="murA"
                     /locus_tag="IV329_001087"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8940287.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1674..1850
                     /locus_tag="IV329_001088"
     CDS             1674..1850
                     /locus_tag="IV329_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8940288.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1985..2281
                     /gene="yidD"
                     /locus_tag="IV329_001089"
     CDS             1985..2281
                     /gene="yidD"
                     /locus_tag="IV329_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8940289.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2465..2932
                     /locus_tag="IV329_001090"
     CDS             2465..2932
                     /locus_tag="IV329_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8940290.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3017..3901
                     /locus_tag="IV329_001091"
     CDS             3017..3901
                     /locus_tag="IV329_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8940291.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4018..4251
                     /locus_tag="IV329_001092"
     CDS             4018..4251
                     /locus_tag="IV329_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8940292.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4339..4419
                     /locus_tag="IV329_001093"
     tRNA            4339..4419
                     /locus_tag="IV329_001093"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4373..4375,aa:Tyr,seq:gta)
     gene            4426..4497
                     /locus_tag="IV329_001094"
     tRNA            4426..4497
                     /locus_tag="IV329_001094"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4458..4460,aa:Gln,seq:ttg)
     gene            5012..5719
                     /locus_tag="IV329_001095"
     CDS             5012..5719
                     /locus_tag="IV329_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8940293.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5779..6423)
                     /locus_tag="IV329_001096"
     CDS             complement(5779..6423)
                     /locus_tag="IV329_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8940294.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6550..6837
                     /locus_tag="IV329_001097"
     CDS             6550..6837
                     /locus_tag="IV329_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940295.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6945..7265
                     /locus_tag="IV329_001098"
     CDS             6945..7265
                     /locus_tag="IV329_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8940296.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7346..8659
                     /locus_tag="IV329_001099"
     CDS             7346..8659
                     /locus_tag="IV329_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8940297.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8656..9375
                     /locus_tag="IV329_001100"
     CDS             8656..9375
                     /locus_tag="IV329_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8940298.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9503..10054
                     /gene="raiA"
                     /locus_tag="IV329_001101"
     CDS             9503..10054
                     /gene="raiA"
                     /locus_tag="IV329_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8940299.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10276..12810
                     /gene="secA"
                     /locus_tag="IV329_001102"
     CDS             10276..12810
                     /gene="secA"
                     /locus_tag="IV329_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8940300.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            12937..14038
                     /gene="prfB"
                     /locus_tag="IV329_001103"
     CDS             join(12937..12999,13001..14038)
                     /gene="prfB"
                     /locus_tag="IV329_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8940301.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14143..14829
                     /gene="ftsE"
                     /locus_tag="IV329_001104"
     CDS             14143..14829
                     /gene="ftsE"
                     /locus_tag="IV329_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8940302.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14813..15706
                     /locus_tag="IV329_001105"
     CDS             14813..15706
                     /locus_tag="IV329_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940303.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15858..17306
                     /gene="cls"
                     /locus_tag="IV329_001106"
     CDS             15858..17306
                     /gene="cls"
                     /locus_tag="IV329_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8940304.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
BASE COUNT         5877 a         2984 c         3836 g         4840 t
ORIGIN      
        1 attataaaac aatcgttaga aaggacgtca ttatgcaagt atacggaatt gatgcggtgc
       61 ttcgaatcgt gagtcatttg gcgtttatct acttagcctt ctggtcttta cgttcgttac
      121 gcattgaaac ttttttcaga ccaatgcata atacgcaaat tagaatggca atcgtacttt
      181 tttcaatttt tttagggtac actgcaagta attttttctt agaagttatc gctttatgcc
      241 gaaatctttt cgttagcctg taaattttaa aaaaaggtac tattttaaaa gcaaggatgt
      301 tataatagaa aagattttga gtaagtgtat gctcattgga attttttgga gggaacaaca
      361 tggaagagat catcgtaaga ggtggcaatc aattaaatgg aacagtaaga attgaaggag
      421 ctaaaaatgc tgttttgcct attttagctg ctagtttgtt agcagaagaa ggtattacga
      481 cattagacaa cgttccgatt ctttctgatg tcttcactat gaatcaagtg attcgtcact
      541 taaacgtaga tgtagatttt gatgaacaaa agaatcaagt aacaatcgat gcttcaagac
      601 aattagaaat cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca atcgttgtga
      661 tgggtccgct acttgccaga aatggacatg caaaagtagc tatgcctggt ggatgtgcga
      721 ttggtaaacg accaatcgat ttgcatttaa aaggatttca agctttagga gcaaaaatta
      781 ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt gatcggtaac acgatttatt
      841 tagatttccc tagtgtaggt gctactcaaa atatcatgat ggctgccgta aaagcaaaag
      901 gaacaaccat cattgaaaac gtagctcgcg aaccagaaat cgttgattta gcaaacattt
      961 tgaataaaat gggcgctcaa gtctatggag ctggtactga aacaatgcgt attgaaggcg
     1021 tggatcattt acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa gcaggtacct
     1081 ttatggttgc tgctgcaatg acacaaggca atgtgctgat tgcagatgct atctctgaac
     1141 ataaccgccc attgatttct aaattgatcg aaatgggtgc agaaatcatt gaagaagaag
     1201 gcggagtccg tgtcatcgga ccaaaacata tcttacctac agatgtaaaa acgatgcctc
     1261 atcctggttt ccctacagat atgcaagctc aaatgacagc tatccaatta gttgcagaag
     1321 gtacaagtgt agtgactgaa acagtgtttg aaaatcgttt ccaacattta gaagagatgc
     1381 gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat ggaaaccatg
     1441 aattgcaagg tgcagaagta tatgcaacag acttgcgggc agcagcagca ttagttttag
     1501 ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt gaattatcta gaccgtggat
     1561 attataattt ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt gtagacatgg
     1621 atcaaacttc tgctgaaaaa acagcacaaa cgattgctta aggaagtggc tgaatgagta
     1681 accaacgata tatcatatta actttgataa agattttagt ggtgatctta ttgcttatcc
     1741 ttctttttgt ggccggaacg atgatcggat atggtgtcat cggtggcggc aatccattta
     1801 aagtattcca gccaagtttg tggattcata ttcgagactt ttttcattga taaataagaa
     1861 tcacagtggt tctagtcgat atgaagtgag ccatgaagca gaacatgaca aaagaaaaca
     1921 gaagggaacg ttgacgttag tcgcgatccc tttttttagt aaaagaaaag aaggaatcta
     1981 attgatgaag aagatattga tttatttagt tagaggatat cagcgcttta tttcgcctgc
     2041 atttccacct agctgccgtt attacccaac ctgttccaat tatatgataa aagcaattca
     2101 aacccatggt gcactaaaag ggacggtaat ggggactgca cggatcttac gctgtcatcc
     2161 ttttgtcaaa ggcggtatcg attatgtacc attgacattc caattaacgc gaaataagaa
     2221 agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat acgagtagca ctgagcgata
     2281 aagaaacaat ttggaaaata gcttcttata ttttgcaaaa ttgtgagtta ttgccgaagt
     2341 gttggatttt gaacaccgtc aaaaaaaggt tgtgaaaaat acgggtagca ctgagcgata
     2401 aagaaaaaga ggaaaattat tgtcaaagta acaactccgc aactacttat atgggggaca
     2461 aaaaatgatc gaagaactaa acaagaaaga tttaccatgg tccttgttat tagatgcaga
     2521 tcctgataaa gaaaaagttc aagtatatgt ttccagaggg agtggctgga tatggaaaga
     2581 aaaagaaaaa acaataggtg tactgatcta tgtggcaagg gaaaaagaat acgaaatcgt
     2641 caatgtagca gtagccccaa cgtatcaagg aaaggggatt ggcggaaaat tacttgagac
     2701 agcttttcag aagctttcac aactcgcttc cagtcaaact cagatcatca tacgaaccgg
     2761 cagtactagc agtgcggcgt tacatctcta tcaaaaaatg ggctatgtgg agattggtcg
     2821 agtcaaagac tattttatcc atcattactc tgagcccatt tttgaaaatg gagatcaatt
     2881 aagggatcaa gtaattttag caattcactt acctaaaaat agctttaact aataaacttt
     2941 caattcgaga aaatgctttc aaatgctaaa tagatacatt acaatagctt tagcaaacaa
     3001 aaaggagaat atttttatgg gaatattagt ttttgatttt ggtggatcgg ctgtcaaata
     3061 tggttgttgg gatggcaaag agattaaagg taagggacaa tttgcaacgc ctgaatcttg
     3121 ggaagaaatg aaagcgcaat tatttcaagt ttataaaaaa atactcgttt cttttgaagt
     3181 cgaaggaatt ggaatcagct ctcctggagt agtgaataat gagaaacagg tgattgaggg
     3241 aatcagtgcg attccttata ttcatggatt taatattttc cgagatttgg aaactctgtt
     3301 tcaactccca gtaacgattg aaaatgacgc caactgtgcc ggaatggctg aattttatga
     3361 aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg gcaggaactg ggattggcgg
     3421 ggcgttgttc acgaaaggac aaatcaataa aggggcacat ctttatggtg gagaatttgg
     3481 gttgatgttc ttaaatggcg ataagacatt cagtaaactt ggtacagcgg tacagatggc
     3541 ttggcgttat tgtgaacgca aaggtctcga taaaaattct tataccggaa aagatgtctt
     3601 tgaattagca gaaactggag atgcaatagc aaaagaagaa gttgagagtt tttatacgta
     3661 tcttacaaag ggattattca gtattcaatt ttcgtttgat ccagaagtga tcgtattagg
     3721 cggcggtgtt tctgcaaaag aagggctcat tgatgagatc aaaagccgaa tgaagaagct
     3781 gacagaacaa ttcgatctgc atgatttcga accgcagatt ttattatgtg aatacagaaa
     3841 cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag gggagaatta
     3901 agagctttag agaaggtgca gcttgcataa aagaagtgaa tgaaatgaat aggagatttt
     3961 tacctaagga atgttttatt tgctgcagta tgataaaata tctgagaggt gaaaatgatg
     4021 tcaaaaaaaa ataaagatgt tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt
     4081 aagaattatg aagtcactca gttatttata gggaaaaaaa tgattggtga gatcttagcc
     4141 tatgggccaa aagaatatga aattttcttt ggtgaagaag attttggaaa agaaaaaagt
     4201 cttgaaaatg cgatagaaac agtgattcga cactggaatc tgcacgaata aaaaaatatc
     4261 aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat taggtattat aactaagtca
     4321 ggttgataca aacctttcgg aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc
     4381 gttccttagg gttcagtggt tcgaatccac tctcctccat ttcattgggg tatagccaag
     4441 cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct accccagtat
     4501 accacaatcg taagctctcg ttagttcgag agctcttttt tttgctttgc taccttgttc
     4561 cttgaagatt ctgctgattt aagatagagt taagttaact acaagaattg gagggatcca
     4621 catgaaatgg aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc
     4681 agaaaaatac aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa
     4741 atcaaaacgc ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca
     4801 aaagatccat aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga
     4861 acatatctaa aaaattgttt ctgacaatag gagtaggaag aacgatgtgt gtctaaatcc
     4921 tctgttgatg gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat
     4981 aatagaagaa acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca
     5041 gattcatgac caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct
     5101 tccttcagaa agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc
     5161 aattcaaaac ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacatatgt
     5221 tgcgcgagaa aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga
     5281 atcgcaaaat cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc
     5341 aagtgaaatg gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat
     5401 tcgtttcgca gatgatgtcc ctatttgttt tgaagttgcc agtgtgccac aaaagctgat
     5461 cgatggctac agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg
     5521 cgggaaaaag atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat
     5581 tgctgactat ttagaggtaa aacgaggaga tgcagttctt cgtttgcgcc agatttctta
     5641 ttttgaagac ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga
     5701 gttttatttg gaaaaataaa aaacaggtga tccctgattc cttacttaga aataaaagag
     5761 aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat aagaaacagg
     5821 accctcttca aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtc
     5881 agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt
     5941 tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat
     6001 catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg
     6061 agagacagca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa
     6121 atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga cttccaacat
     6181 cggtacgcca gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt
     6241 ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc
     6301 ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac agataaagcg
     6361 tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat agcgctcgat
     6421 catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt ctaggttgtt
     6481 tcaacactgt aacggttgcg caatcgctgt agtatgcgat aataaagaca aaggtaggtg
     6541 gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac
     6601 aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct gtttcccagt
     6661 aacggacttc ccaaaacagc aagggaaagt acagattggt gggcaaatga ttataaacac
     6721 cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt catcaatttg
     6781 gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt tgattaaata
     6841 aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag taacagacgc
     6901 gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa
     6961 tcatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa atgcaaaaag
     7021 ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct gacgcagata
     7081 acaatcttgc aaataaagat gtagatgtac tgttattagg acctcaagtt cgctttatga
     7141 aagctgattt tgaaaaacgt ctggaaccaa aaggcattcc tttagacgtc atcaatatgg
     7201 ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg ctcataggtg
     7261 aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt
     7321 cgtatatttt tttagggcga ataatatgaa tcatttttgg ggtagaaggc ttctgacgag
     7381 agatattaaa gcaatagact gtgaggaggt aaatttacca aattatgaga agatcgcagc
     7441 agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga cgtccgtcat
     7501 tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt tagggcgatg
     7561 cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt
     7621 ctttttttgg acaggacagc ttactgaaaa acaaacggaa atcgcaaaga gaatactttg
     7681 ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga aaacagagat
     7741 gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga tttgtacacc
     7801 gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc cagaagaaaa
     7861 aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt ttgttatctg
     7921 tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga tcgatgagat
     7981 cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa cagcgattaa
     8041 accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac taaaggaaat
     8101 caagcagacg tttgacatcc acaaacttgc tttgcgcttt catcggcgtt tacttcctga
     8161 acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga agaagataaa
     8221 atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt tattttgtcc
     8281 aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc cgagatatga
     8341 gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga aaatgcgaga
     8401 agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca catttgaaag
     8461 aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt ctttggtaca
     8521 aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat actttttcta
     8581 tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga
     8641 agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca
     8701 ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc agtttgtcgc
     8761 gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc aatccatttc
     8821 aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag tgactgtcaa
     8881 gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt tgtgtacgat
     8941 gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg actagcaggc
     9001 acctttgcat ataaatggac gcagataaaa aaagaatacc cgacatacat ctattgtcca
     9061 atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag cgagatgttg
     9121 aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct gctggctcaa
     9181 gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact tgcagtcacc
     9241 ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac aacaggacag
     9301 actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt
     9361 tctttagcaa gatgaattag atgtggaaat ttgatagcga tttaatttgc aaataaccta
     9421 gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt cttgattgtg
     9481 gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa atatcgaagt
     9541 aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac gctatttcag
     9601 tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa
     9661 agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa cttctccaga
     9721 tttatacgca agtatcgatt tggttgttga caaattggaa cgccaaatcc gcaaattcaa
     9781 aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg ctactgcttt
     9841 attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac gtacgaaacg
     9901 cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca tgctgggaca
     9961 taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt accgccgtaa
    10021 agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa
    10081 acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct cttttagatg
    10141 aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc aattatctat
    10201 aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca gaaggaaaag
    10261 gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga taagaaagaa
    10321 ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca aatggcagca
    10381 ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata ccaaaaagga
    10441 gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc agcaaaacgt
    10501 gtattggggt tatatccata ccacgtacaa ttaatgggtg ggatcgtgct tcacgatggg
    10561 aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ctgcaacgat gccggtgtat
    10621 ctgaatgcgt tgactggcga aggggtccac gtagttaccg tcaatgaata tttagcaacc
    10681 cgtgactcga cggaaatggg cgagctttat aatttcttag gattgacagt tgggttgaat
    10741 atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgcgatat cacttatagc
    10801 acgaataatg aattaggatt tgactatttg agagacaaca tggtcgttta ccgtcatcaa
    10861 atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcgat cttgattgat
    10921 gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatcaacagc tttatataca
    10981 agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat cgatatacaa
    11041 tcaaaaacaa tcagtttaac tgaagcaggg atcgaaaaag ctgaagaaac attcggtttg
    11101 gacaacttat atgacattga aaatactgca ttgacccatc acttggatca agcattgaga
    11161 gccaactaca tcatgctttt agacatcgat tatgttgttc aagatggaaa agtattgatt
    11221 gttgaccaat ttacgggtcg gatcatggat ggacgccgtt attcagatgg tcttcatcaa
    11281 gcgatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaacgat ggcgacgatc
    11341 actttccaga actatttccg tatgtacaag aaacttgcgg ggatgactgg tacagctaag
    11401 acagaagaag aagagttcag agagatttat aatattcaag tataccagat tccgacaaac
    11461 cgtccggtca tccgtgacga tcgaccagat ttgctttacc caacattaca aagcaagttc
    11521 catgctgtcg tacaagatat caaagatcgc taccgcaaag gacagcctgt cttagtcgga
    11581 actgttgcag ttgaaacatc tgagttatta tcagatatgc tgaatcaaga acgtattcct
    11641 catgaagtac tgaatgcgaa aaatcacttt aaagaagcag aaatcatcat gaatgcgggt
    11701 caaaaaggtg cagtgacgat tgctaccaac atggctggtc ggggaacaga tatcaagcta
    11761 gggcttggcg taagagaact tggtggccta gcagttatcg ggacagaacg acatgaatct
    11821 cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca
    11881 caattctact tgtctttaga agatgatttg atgaaacgat tcggttcaga acgaatcaaa
    11941 gctttcttag atcgaatgaa gatcggtgac gaagatgctg tgatccaaag caagatgctg
    12001 acgaaacaag tagaagctgc acaaaaacgc gtagaaggaa acaactacga cacacgtaag
    12061 aacgtcttgc aatacgatga cgtgatgcgt gagcaacgtg aagtaatcta taaacagcgt
    12121 caagaagtga tcatggaaga aaaaagctta tctaaaccac tgatgaatat ggtcaaacgg
    12181 acaatcagtc gtgtggtaga tgcgcatacg caattagaaa aagaaaattg gaatttggaa
    12241 agtatcgtcg attttgctgg cagcacgctt gtccatgaag acagtatttc ccttacagat
    12301 atcgaaggaa aaacctctca agaaatcaaa gaatatctaa acgaacgagc acaagaagta
    12361 ttcaatacca aagcagctca attgaacgga ccagagcaac tgcttgaatt tgaaaaagtc
    12421 gtgatcttac gtgtcgtgga ctctaaatgg acagatcata ttgatgcaat ggaccaatta
    12481 cgtcagtcaa tcggcttgcg tgcctatggt caaaacaatc cattagtcga ataccaaaca
    12541 gaaggttata agatgttcga agatatggta ggtgccattg aatatgaagt cactcgtatc
    12601 ttcatgaaag ctgagatccg tcaaaacgta caacgtgagc aagtttctca aggaacagct
    12661 tctcactcag aagatggaga tgccaaagaa aacagtaata ctagcgcgaa aaaacaacca
    12721 atccatgtga acaaggttgg tagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat
    12781 aagaactgtc acggaaaaga tgcaaactaa tttctgacag caggattggt gagacaaatt
    12841 cttttttaaa gaatttgtct caccttcttt ctgttatttg acgattctgt tagactagat
    12901 ataataaaaa aagtatagtg aaagaaggaa aaaaacatgg aaatatccga aattcgaaat
    12961 ctcctgacag aaatgcagga aaaggtcaca agcttcagga ggtctctttg acttagacca
    13021 gctggaagaa gacattgcgg aagcagaagc aaaaatggct gaacctggtt tttgggatga
    13081 tagcgaagct gctcaaaaag tgatcagtga aaacaatgca aataaagaaa cttatgatca
    13141 attccattct cttgctgaag aactggaaga aatcgaattg atggtggaga tgttgcagga
    13201 agaacctgat gcagtaatgc aagaagaagc agaagcacgt atcaatgcgc tagatgaaaa
    13261 aatgaatacg tatgaattat ctatgttgtt agatggtcct tatgatcgaa acaacgccat
    13321 cgtcgaattg catccagggg ctggcggaac ggagtcccaa gactggggca gcatgctttt
    13381 acgcatgtat acacgctggg cagagcagca tgggtatcaa gtagagacac tagattatca
    13441 agcaggcgat gaagcaggaa tcaagagtgt gaccctactg atcaaaggac acaacgcatt
    13501 tggctatctg aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc cttttgactc
    13561 agcaaaacgt cgtcatactt ctttttgttc ggtagatgtg atgccagagc ttgatgaaac
    13621 gatcgatata gaaatcaatc cagatgattt gaagatcgac acatatcggg cgagtggtgc
    13681 aggtggtcag cacatcaata agacagaatc cgctgtacga atcactcact tgcctacagg
    13741 aacagtagta gcgagtcagg cacaacgttc acaattgaaa aaccgggaac aagcgatggg
    13801 gatgctgaaa gctaagcttt accaattaga agtggaaaag aaagaacaag aagcagctgc
    13861 actgagagga gaacaaaaag aaatcggctg gggatcacag atccgttctt atgttttcca
    13921 tccatattct atggtaaaag accatcggac aaacgtagag acaggaaatg tccaagctgt
    13981 tatggacgga gatcttgatt tatttattga cggctatttg aaaatgcatc tgcaataaga
    14041 gccctttgtt aaattgaaat aaaattgtaa gattttgcaa gcaagttgaa acattgccat
    14101 gctataatag ctaaagactg aaaaagatat ggagagaacg ccatgattga aatgaaggat
    14161 gtaatgaaga agtactcaaa cggtaccact gctatccgca atctctcgat tgtcatcgaa
    14221 caaggagagt ttgtgtatgt tgttggtccg tctggagctg gtaaatctac attcatcaaa
    14281 ttgatgtatc gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg tcatgatcta
    14341 atagccatca aaaaccgcga agttccttat ctacgcagag aaatcggcgt ggtgttccaa
    14401 gactataaat tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc catgcaagtt
    14461 atcggcagaa aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga tcttgttggt
    14521 ttgaaacaca aagtccgagt ctttcctagc gaattatctg gtggggaaca acaacgtgtt
    14581 tctattgccc gagcaatcgt aaatacacct aaagtactta tcgcggatga accgacaggg
    14641 aatctagatc cggaaaactc atgggaaatc atgaaactat tagatcggat caatgcacaa
    14701 ggaacaactg tcgtgatggc tacacataac agtacgatcg taaatacgat tcgacaccga
    14761 gtaattgcta tcgaaaacgg ccgcattatt cgagaccaag cggaagggga atatggatac
    14821 gatgattaga acctttttcc gccatttatt agaaagtatc aagagtttga aacgtaacgg
    14881 ctggatgaca gtagcttcgg tcagtgcggt aacgatcacg ttgactttag ttggtgtgtt
    14941 catggcggtc atcatgaacg caaccaaact agcacaagat atagaaggaa acgtggatgt
    15001 atctgtgttc gtagacattg gtacaaaaga cgaagagatg aaaaaattag aaaaagaatt
    15061 aaatgactta gatcatgtca aaaaagtcga attttcaagc aaagaacagg aattgaagaa
    15121 aatccaaaac gaaatgggcg attcttggaa attgtttgaa ggggataata acccgttata
    15181 tgatgtatac gtagtaagtg ctaccgatcc ttcttataca aaacaagtag cagaaaaagc
    15241 ggcagatttg aagaatgttt cccgttctga ttacggtggg gcttcctcag atcggatttt
    15301 ccaaattgct ggtgcagttc gaacatgggg attagttgcg gcggcattgc tgctgtttgt
    15361 agcaatgttt ctcatttcta acacgattcg gatcacgatt ttatctagac agcgtgaaat
    15421 tcaaatcatg cgtttagtag gggcaaaaaa tggatatatc cgctggccat ttttccttga
    15481 aggtggttgg atcggcttaa tcggtgccat tcttccgata ctgatcatta catttggtta
    15541 cgcatggttt taccaattga tcaatccgtc attattacga tctcattatt cattgatcca
    15601 tccagaaaat atcgtttgga aaatcaatct tttgatggtc ggtattggtg tgatcattgg
    15661 ttcactcggc tcgatcatct ctatgagacg tttcttgaaa ttctagtgtc acaagataaa
    15721 tataatcaga aagttggacg atttcgttcc agctttcttt ttttatcagc taggtggagt
    15781 aaatggggcg gcttttttct ctttacttta aaaaacaact tgggtattct taagttggta
    15841 gaaaaagagg tgaagccgtg aagattatcg ttgacaattt ttttacgatc cttttgatta
    15901 tgaatatttt gctttccttt attatcgttt ttcgagaaag aaaggaaact gcgcaaacat
    15961 gggcatggct gctcgttttg atgttcattc cagttgtagg atttatttta tatatttttt
    16021 taggaagagg gatttccaaa gagaaaatct ttgatttgaa gatccaaggg aaaatcggta
    16081 agaatctaga aatcgaagaa gataaacaag cattgatgcg agggctttat cctcatccgc
    16141 ctactggaca agtagatgtg aaacaattga tctatatgct tactgtattt gaaagtacgc
    16201 tttatacgac taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc
    16261 tgatcgagga tatcaaacag gcagaagacc atatccattt ccaatattat atttaccgca
    16321 gtgatacatt aggtgaagaa gttcgagagg cattgacaga ggcagcaaga cgcggagtaa
    16381 aagtcagagt ccttcttgat gcatggggtt ccacacaagt ttcttctagt ttttttcaga
    16441 atttgaaaaa agcaggtgga gaagtcgcat ttttctttcc tctattcgtt ccgtatatca
    16501 atcctcgaat caattatcgc aatcatcgta aaatcgtagt gattgatggg aaaatcggat
    16561 atacaggtgg gttcaatgtt ggaaacgaat atctaggatt ggtaaagaaa tttggttatt
    16621 ggcgagacaa tcatctgcgt atctatggag aagcggttta caccttgcaa aaccgttttt
    16681 tgatggactg gaattctcag catacaaaag aagtaggata ttcaccggaa tttttcccga
    16741 ccattcattc tacaggtgat atggctgttc aaatcgtgac aagcggacca gataccgaac
    16801 atgagcaaat caaaatgaca tatttgaaaa tgatttcgtt agccaaacgg gagatattga
    16861 tccaaacgcc gtattacata ccggatggat cgattcatga ggcattgaag ttggctcttc
    16921 tctcaggcgt aaaagtccat atccaaatac ctaacaaacc agatcatctt ctcgtttact
    16981 gggcaacgta ttctttcgct gcagagctga tcgagtatgg ggcaagaatc gaaacatatg
    17041 aaaatggatt tatccatgca aagacgatga ttatcgatgg cggcatcgtt tccgttggtt
    17101 cagctaatat cgacgttcgc tcatttagat tggattttga agtcaataca ctgatctatg
    17161 atgaacgaat ggcttctcgc gtaagaaaag cattctttga agattcaaag atttcgacgc
    17221 atctcacaaa agaagtgtat gaaaaccgag ggatgatgat caagatgaaa gaaggattag
    17281 caagactgat ttcgccgctc ctttaaaata caaaaaggac aggagagcca tcaatagatg
    17341 gaaatctcgc gtcctttttt ctcggggcca tttctgagag tacgtcagaa atggagaaag
    17401 cgtctcgcat gagcgaaatt aactttgggg tatctcttgt tttttgggga aagcaagaaa
    17461 taagctaata tatgctatta aaagagacac atttcaaagc cagtatagca tagaaacttt
    17521 taatgagata gcttttt
//
LOCUS       DACDKL010000043        17457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDKL010000043 DACDKL010000000
VERSION     DACDKL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.043"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            76..1017
                     /locus_tag="IV329_001107"
     CDS             76..1017
                     /locus_tag="IV329_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8940305.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(1167..2063)
                     /locus_tag="IV329_001108"
     CDS             complement(1167..2063)
                     /locus_tag="IV329_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8940306.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            2332..3672
                     /locus_tag="IV329_001109"
     CDS             2332..3672
                     /locus_tag="IV329_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8940307.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            3890..4333
                     /locus_tag="IV329_001110"
     CDS             3890..4333
                     /locus_tag="IV329_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8940308.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="IV329_001111"
     CDS             complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="IV329_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8940309.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(6658..7881)
                     /locus_tag="IV329_001112"
     CDS             complement(6658..7881)
                     /locus_tag="IV329_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8940310.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(7912..8751)
                     /locus_tag="IV329_001113"
     CDS             complement(7912..8751)
                     /locus_tag="IV329_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8940311.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(8768..9448)
                     /locus_tag="IV329_001114"
     CDS             complement(8768..9448)
                     /locus_tag="IV329_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940312.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(9450..10523)
                     /locus_tag="IV329_001115"
     CDS             complement(9450..10523)
                     /locus_tag="IV329_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8940313.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(11170..11841)
                     /locus_tag="IV329_001116"
     CDS             complement(11170..11841)
                     /locus_tag="IV329_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8940314.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="IV329_001117"
     CDS             complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="IV329_001117"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8940315.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            15265..15702
                     /gene="ndk"
                     /locus_tag="IV329_001118"
     CDS             15265..15702
                     /gene="ndk"
                     /locus_tag="IV329_001118"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8940316.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(15724..16590)
                     /locus_tag="IV329_001119"
     CDS             complement(15724..16590)
                     /locus_tag="IV329_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8940317.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(16750..>17457)
                     /locus_tag="IV329_001120"
     CDS             complement(16750..>17457)
                     /locus_tag="IV329_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8940318.1"
                     /translation="YLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEF
                     DNAQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQ
                     HDQVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLS
                     DKLNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTT
                     NLYPVTRTDVEQQKNNQ"
BASE COUNT         5077 a         3577 c         3090 g         5713 t
ORIGIN      
        1 aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata
       61 aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc
      121 ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac
      181 attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca
      241 aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc
      301 gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt
      361 actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa
      421 aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
      481 ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca
      541 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg
      601 atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg
      661 aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca
      721 gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc
      781 atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
      841 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
      901 gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
      961 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
     1021 gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa
     1081 gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta
     1141 cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg
     1201 tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca
     1261 taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat
     1321 tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga
     1381 gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc
     1441 aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt
     1501 tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga
     1561 atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt
     1621 tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa
     1681 aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc
     1741 aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc
     1801 gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat
     1861 gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga
     1921 taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg
     1981 gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact
     2041 agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg
     2101 atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta
     2161 acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt
     2221 ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta aatattgcta
     2281 tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt tatggaagat
     2341 aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt aggtggagca
     2401 atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg cccttctgta
     2461 ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa
     2521 atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg cccttatcta
     2581 ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat tatcaatatc
     2641 atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca cattccagcc
     2701 tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta ttcggtacgg
     2761 ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat tatcttactg
     2821 atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt tttccctacc
     2881 cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg aatcatcagt
     2941 tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat cacggtaagc
     3001 gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat gggaagaatc
     3061 atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg gaatacttta
     3121 gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat cccatttgca
     3181 gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa ttcgggtgtg
     3241 tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa
     3301 ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg taccagtgtt
     3361 ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg ctatctggca
     3421 ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc atcctttgta
     3481 ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc attagctgtc
     3541 ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac tgtattgact
     3601 ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg
     3661 acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga tggagctatt
     3721 gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata aacagtctca
     3781 tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat tgttctccat
     3841 gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa tgaaaaagta
     3901 ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta tgatgtactt
     3961 gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca tggcgttgat
     4021 gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt atgaaaacaa
     4081 aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt ccttgtttat
     4141 ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga ttcctcatca
     4201 ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag taaaaaaatt
     4261 agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa aaatgatcaa
     4321 agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag aagttatcag
     4381 cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt gtgaaaaagc
     4441 tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat ttttcgtttg
     4501 gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtaaaat
     4561 cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca tattttgcca
     4621 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga ggttgtgaaa
     4681 aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca catttttgta
     4741 taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt aaaagggatt
     4801 gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt gcgctgtctt
     4861 gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg tgaaggcact
     4921 gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt
     4981 aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca ctaagtagtt
     5041 gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa atcctgcttt
     5101 ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt gttcaccaat
     5161 aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg gtgtgttcgt
     5221 catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga tcaacaatcc
     5281 acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt cgattcctcc
     5341 gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc ctgattcgat
     5401 gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca ttcctactgc
     5461 tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg cacacagtaa
     5521 cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt ctagaacatc
     5581 agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta aaatcatgaa
     5641 taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct tgatgtgttt
     5701 gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata atggagacat
     5761 cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg gatttaagcc
     5821 tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact
     5881 ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa accactggac
     5941 actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt gtatccatac
     6001 agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg gtttgatgcc
     6061 attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg catacaacat
     6121 gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc cgacaacgcc
     6181 aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa aacttggtgt
     6241 tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta
     6301 agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa aataaaaaga
     6361 cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt
     6421 ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg tcaattaggc
     6481 ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc tactgcgtga
     6541 aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata
     6601 ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta ttttttacta
     6661 gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt gcttgattct
     6721 ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta gttctgctgc
     6781 aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt cttctgtgtg
     6841 tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta tgacttggct
     6901 gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta tttcttcatc
     6961 tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag gagtaacgat
     7021 gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc
     7081 agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt gccgatttcc
     7141 tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag ctactcctcc
     7201 gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat
     7261 tccattaaac acttggatca atgcttccaa agcatttact cctttttcag gtgctgctgc
     7321 tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta aagattctgt
     7381 cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat cctcaaaaaa
     7441 accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt cccctggggt
     7501 gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta tgctggcggc
     7561 taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac ctggtaaagc
     7621 atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat aacgtgcatc
     7681 aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt tttctaattg
     7741 tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat gttcgtggat
     7801 atctagagca agtgcttgat attctggtaa acgatattgg attagatcct tcaattggtt
     7861 tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt cttattttct
     7921 cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga ttactttgac
     7981 tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt tttcatcttc
     8041 ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg gttctaagaa
     8101 gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat tgatcactga
     8161 aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag aaatgtctag
     8221 attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg ccttatccac
     8281 tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg taacatcatt
     8341 agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag tgttagaata
     8401 aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc cgtgttcttt
     8461 attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg atccatctgc
     8521 taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat taatgttctc
     8581 ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa taatccctag
     8641 ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac accctgccaa
     8701 ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca ttttttctcc
     8761 tccactttca atgtgtaatt tttttaatca gttgattact gataaattga cttaaaaaaa
     8821 ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat cgattatacc
     8881 ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc atggtagtca
     8941 gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc aaaccttcta
     9001 acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct gccgcttcga
     9061 tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa
     9121 ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca actaagaaac
     9181 gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga atgatattga
     9241 tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta gggcgtgtca
     9301 ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct gtccaaaaag
     9361 acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa taaacattgg
     9421 gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac tttgatacga
     9481 ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat caaataatcc
     9541 aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact ccctaacggc
     9601 gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg atatttgctg
     9661 tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa ttccaccagc
     9721 catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg
     9781 tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg attgctgaag
     9841 atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc taccttgttg
     9901 cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa acctaactgg
     9961 cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg
    10021 gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc gattgctact
    10081 cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc ttccaaaccg
    10141 acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt tccagaatac
    10201 tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt gaaatgctga
    10261 aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag ctgactcaga
    10321 ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt catcacacga
    10381 actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc gcctttttca
    10441 atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt gaaacataca
    10501 gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc gtcctcaaga
    10561 aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc atgttcaagc
    10621 tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata tcggtcgcag
    10681 ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt tccaatagtc
    10741 gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta tcgtacttac
    10801 catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt
    10861 aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct caagcccaac
    10921 atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa tttgcctact
    10981 tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc ttctcaccag
    11041 ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat cgatattttg
    11101 ttgttgggat taatctacat gaggaaaact gaaaagtcaa gtacgaaatt caaattattt
    11161 tttctatatt tatttttctg tttttttatt taacatttca ataacagttc gaagatcttt
    11221 tccttccgca agaagtttca atttttcctt ttcttcgacg gtataatttc ggacaaaact
    11281 ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa
    11341 agaatcttcc actgcaaagg ctgaactagc ttctaatcct gttcgttcca aagctgtcag
    11401 ataaggaatc ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa
    11461 gtaatcagct aatttaaagt gatccaagat atctgttgct cttttcgtaa cagtggtcgt
    11521 agcaatgcct agtttcatat gacgttcttt taagtctgcc aatatttctt ttgcatatgg
    11581 ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg
    11641 gacttcctgt actttttcag ggctacctgc tgctttcact aaaatatcaa aactttgttt
    11701 ccagtttttt ccgccccaag cgtcaacgat ttcttttggg atatggatat caaattgctg
    11761 aaatccccaa agccagccat cacgatacgt tttttctgtg tcaactaaca aaccatctaa
    11821 atcaaatatt gcacctttca tctatacatt aatcctttcc aaaacaatac acatagttta
    11881 gcacgactgg tccaaaaaga acagaagcaa agaaccagcc ttctcgttta taagagttct
    11941 tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata agcgatgact
    12001 gtagcaaata accataacca gtaattgcca ggaagtggca tcaagcctaa ttcagcacca
    12061 aaacttgtaa aaggaaggac agaaccgaca ataattccta atgtagtgat aacaaacatg
    12121 gcaaatgttg cgttgctttg gataaatggt actttcggtg tacgcagtgc atggagcact
    12181 aaggtttgtg tccatagtga ttcgacaaac catcctgcat ggaataatgc gatgaaaatc
    12241 actcgctgtg aactatccaa tgtatggaaa cttccgccca ctacagcagg acaaataaca
    12301 aaatacatca acaagtaagt agtaatatca aatatcgaac ttgttggacc aaagtaaacc
    12361 atgaattccc caatactaga tgcattccat ttttttggct cgtccaaata ttctttatcc
    12421 atccgatccc atggcaagga aatgcaggaa acatcataga tcagattcaa aaacaaaagt
    12481 tgtaaaggca gcattggtaa aaatggcagg aacgtacttg caatcagcac ggaaaacata
    12541 ttgccaaaat tagaacttgc tgtagccttg atatatttca tgatattccc aaaagtttct
    12601 cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct
    12661 gcagattctt tcgcaatatc cacagctgta tctacagaaa taccgatatc agccactttc
    12721 attgcaggag cgtcattgat accatctcct aaaaatccga cagtatgacc atttttacgt
    12781 aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt ttacgaatac ttcatggtct
    12841 tcagctactt gtgctaattc atgatcactc atttccgaga ttttctcacc ggtaaccaat
    12901 tcattgatct ctagacctac ttgacggcac actgaacgtg taactaattc gttgtcccca
    12961 gtcagaactt ttacagctac accatgttct ttcagagctt tcaacgcttg tttcgttgtt
    13021 tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga ctcatctttg
    13081 actgaaaatt ctccaacgac gcttggattg gtcttttgtg cgacaccaat cactcgtaag
    13141 ccatcttcat taagtgactg tacttttgca agtacggctt cttttcgttc ttttgttaat
    13201 ggtgtaacag taccgccaag atcaataaat ttagaaatgt tcagcatttc ttcaatcgcg
    13261 ccttttgtga tcatttgtgt tttaccagct ggatcagcaa ccacaacact catacgacga
    13321 cgttcaaaat caaaaggaat ttcatccact ttttgataat tgatcttatc tgtatctaaa
    13381 gtttctgttg cttcatcaat gatagccaca tctaacaaat ttttcagccc tgtttgatag
    13441 tagctattta gatacgcatg gcgcaaaaca cgatcatctt catttccttc acaatccata
    13501 tgatattcaa gaatgatttt atcttgtgtc aatgtccccg ttttatccgt acataacaca
    13561 tcgattgcac caaagttctg gatggcattc aaatttttga tcactgttcc ttttttggcc
    13621 atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt
    13681 agaccaactg ctacagatag tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc
    13741 ccattgatga cgataactgc tggagccatc aatgacataa atttgatcag tagcatggat
    13801 gttttatgga tgcctaattc aaagcttgat tgaattggtt tttcagatag cgtttctgct
    13861 acatgaccaa atagtgtttc atttccaaca gaaacaatga ttccttccgc acttccactg
    13921 atgacattac ttcccataaa gacaagattt tcgtaacttg tttcgctttg ctgatcgtct
    13981 tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc tgcttgggaa
    14041 atgaataaat cctttgcgga caataaacgc atatccgctg gtatcatatc ccctgctgaa
    14101 agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtgta ctttccatta
    14161 cgttttacag ctgccgttac tttcacaagt gatttcagtt tttcagccgc ttgattggaa
    14221 cgtacggact gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa gataatggct
    14281 acaccgaaaa gatctttatc tccaggagct gctaatacat attccgtgat gaatgaaata
    14341 attcctaatg cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa tatttctaca
    14401 atgaatggtg tttttttgcc atatgaaatc gcattttcac cgtaattttc tcttgaacgt
    14461 tcgatttgtt cttctgtcaa tccatttgct gatgcatgga atagttcaaa taattcttgt
    14521 ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt ttccatctca
    14581 atctctcctt tgtgtccaaa agacagctgt tctcgctgct tttggtcttt agctattcat
    14641 cggatcgata gagaaaaaac tgaaaggggc tgagaacaga tagataatag ccctggcctt
    14701 tcctctagat ccttgttttt cgaatgactg tcatccattt gttctcatct cctttactaa
    14761 gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tatttttcca attagaccac
    14821 ctccttttta tttttcaccg atggggattt gcggcaacaa aaaaacctct cgccaaatac
    14881 tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct cagatctcat cgttgagttt
    14941 tgacactgta caacgtaaaa agcagatacc tgcttttagc taccttatga aaagccttat
    15001 ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag tagcctatgt
    15061 ttgtatagga gcctcaccta acaaatctta ttcggttttc cttcgttaat ataacaacta
    15121 aaaaagagga tgtcaaatca attttccatg tttttcatac tttttttaaa atacacacgt
    15181 tatattcctg tctcacgcgg ttttatttgt ctcattttcc acctcccgct ataatagaaa
    15241 tatagtagga aacgaggtgc tgttatggaa agaacacttg ttattatcaa gccagacggc
    15301 gtgagacgcc atttagtcgg aagcatcatc caacgttttg aagcaaaggg actggccatt
    15361 gctgaaatga agtttgaaac tatgactcct gaattagcaa aagaacatta tcagcacttg
    15421 actgagcgca gtttttttga tgaactgatc gattacatga cttccggtcc tgtcgtttat
    15481 cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggttgg agcaacaaaa
    15541 gctgccgatg ctgttccagg tacgatccga ggagactacg cgctgccggg gactgaaaac
    15601 atcattcacg cttctgattc ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg
    15661 ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct
    15721 ggtctagttg ctgggttcag caatggccag cagttttttc tctaaggacc tccgttgatt
    15781 ctttcctaac atatattcga gcactgtgaa gaattcttgg tagtctttcg tcttttcttc
    15841 ccctaaagca gcagaaatca atctttgata ataatctgcc atcaatgttt catagatcac
    15901 tgtacttctt tctttatgtg ctaagctccg tttcatatct tcattcacac tacgtgcctt
    15961 tttcaaccga ccattttcta tataaaacga agtcaggttc attaaaaaag taaagagata
    16021 ctgctcatga ttgttcaact gtgctaatcg tcgcattctt tttttgattt tcaaataatt
    16081 gttctcaatg aaccgatcac tgaatatcca aggaaaatca gctagaatag atagttcaaa
    16141 ttgaccatag tctggtatct gcatcaagta ttcttcaatg attcggcatt cataacgtaa
    16201 aaattcaaat tctgtctcag atacttctgt tttttcgacc atgtgaagat tcaaaaccaa
    16261 ataatacaga tcttctgttt tcttaaaatg cctacggtta ttgctgatct ctttataagg
    16321 atcaaagctg tcttcttgta cccgatctag cattcgtctt tttttcttta actttattgg
    16381 ttcatccagc tggtttcgca tccattcata ttctgcaagt gtcatgttca ttcgctctaa
    16441 aaatgatctc aatgttacaa atgacatttt tttcactgaa tttgtctcaa tcccgactag
    16501 agaagtctta aaacagattc cttccgctaa ttgggactgt gtcaacttct tgttgactcg
    16561 aagtttcttg atcaattcac catcatacat aatagttctc ctttttaatt taaatttttc
    16621 cacattcaat aaatagatac gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt
    16681 tttttcataa aaaataccaa ccttcaagca ttagaatgtt gtctaactac tgaaggttgg
    16741 tttgattgtt tattgattat ttttttgctg ttcaacgtct gttcttgtta ctggatataa
    16801 gttggttgtt gttttattac cattttttga aaagacactt gtcgaatgtt cgcctttttg
    16861 cttttcgatt gcttcattac gttccgcttc atttttatag tcatattttt tcggatcaat
    16921 cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca gcttatctga
    16981 aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcat cgattcctgc
    17041 tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc ctaggacggt
    17101 gtatttgggt gtcacaaagt tcccatttcg aaaagcaact acttgatcat gctgagaaga
    17161 taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata gatgtagtaa
    17221 tgtcggcaaa acatcgattt ctccaccgta ttcatgattg actgtccctt tagtataacc
    17281 aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt catcccatgt
    17341 atctggatct ttaccaagag cgcttgctaa atctttatta tctgtattcg agatcccaaa
    17401 atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt tcaaata
//
LOCUS       DACDKL010000044        17070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDKL010000044 DACDKL010000000
VERSION     DACDKL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.044"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..341
                     /locus_tag="IV329_001121"
     CDS             3..341
                     /locus_tag="IV329_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940319.1"
                     /translation="MYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAF
                     GIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSF
                     IVWMFYLVKK"
     gene            436..1320
                     /locus_tag="IV329_001122"
     CDS             436..1320
                     /locus_tag="IV329_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8940320.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1544..4711
                     /locus_tag="IV329_001123"
     CDS             1544..4711
                     /locus_tag="IV329_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940321.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4731..5276
                     /locus_tag="IV329_001124"
     CDS             4731..5276
                     /locus_tag="IV329_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP8940322.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5304..5759
                     /locus_tag="IV329_001125"
     CDS             5304..5759
                     /locus_tag="IV329_001125"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP8940323.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5756..6514
                     /locus_tag="IV329_001126"
     CDS             5756..6514
                     /locus_tag="IV329_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8940324.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6600..7340)
                     /locus_tag="IV329_001127"
     CDS             complement(6600..7340)
                     /locus_tag="IV329_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940325.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7401..8654
                     /locus_tag="IV329_001128"
     CDS             7401..8654
                     /locus_tag="IV329_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8940326.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            8976..9203
                     /locus_tag="IV329_001129"
     CDS             8976..9203
                     /locus_tag="IV329_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8940327.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9326..9652
                     /locus_tag="IV329_001130"
     CDS             9326..9652
                     /locus_tag="IV329_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8940328.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9720..10433
                     /locus_tag="IV329_001131"
     CDS             9720..10433
                     /locus_tag="IV329_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8940329.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10743..11594
                     /locus_tag="IV329_001132"
     CDS             10743..11594
                     /locus_tag="IV329_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8940330.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11715..13580
                     /locus_tag="IV329_001133"
     CDS             11715..13580
                     /locus_tag="IV329_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8940331.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13628..15037
                     /locus_tag="IV329_001134"
     CDS             13628..15037
                     /locus_tag="IV329_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8940332.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15145..15846
                     /locus_tag="IV329_001135"
     CDS             15145..15846
                     /locus_tag="IV329_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8940333.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16005..16484
                     /locus_tag="IV329_001136"
                     /pseudo
     CDS             16005..16484
                     /locus_tag="IV329_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5694 a         2877 c         3681 g         4818 t
ORIGIN      
        1 atttgtatat aaaatatatt catcaatttt ccgttttggt gttaggcaca ctaattatag
       61 cggggattgt cgcatttatt attcaaggcc gtgacatatt aacagtacat caagagtttt
      121 tcagttatgc atttggtatt cctctgatcg ctataaggtc tgataacaaa aagtggaaga
      181 aacgctatta cattctcttg ctatttttgt gtttttatct gccacctaag atttttactt
      241 tattaggtat acaatgccta tcattttctt ttatttccta tatcattgtt ttaattttcc
      301 tcagtttcat agtatggatg ttttatcttg tcaaaaaata aatgaatgtt tcatgaacag
      361 tacttcatca cagcctaatt tttttcttta tgctatcgtt atattaaaga aatcaatcag
      421 aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa accccagttc aggcggtgaa
      481 caagcaaagg aatttgagca attagcgata gcaaaattgg aatcggtgtt tgatgaagtg
      541 gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact ttactcgcga agcggctacg
      601 gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa cagtcaacga aggaatcagt
      661 ggaatcgctg aacaagaaca tcggccaaat ttcggatttt tcccattggg tacggtgaat
      721 gacttggctc gagcattagg gataccgcta gaacctgagg aagcgatcaa tcatttttcg
      781 attgaatcag tcaagccatt ggatattgga aaaataaatg acgactattt tatgaatgtc
      841 gtcgcaattg gctctatccc tgaatcaatc aatgatgtag atgcggagaa gaagacgaaa
      901 tttggaaaac tagcttattt catgtcagga atcaaacagt tagcttccac acaatcctac
      961 tcgtttcacg tagaagttga tggaaagaag gaagaaattg agagcagtac cttgctgata
     1021 ggactaacta actcagtcgg cggttttgaa acattacttc caaatgcaaa agtggatgat
     1081 ggtaagctcc atttggttta tttaaaagat tcttctttga tcgatacgct taaaacattg
     1141 ccagacctat taaaaggagt agatgaatct acgaaaaatc tagcctacct tacttgtgag
     1201 caattagttg tgtcattagc tgatgaggaa gaattgacga cgaatgttga tggagatgaa
     1261 ggggataaat tacctgtgac aatcaaagta ttgccctcac atttacaggt ttattgttaa
     1321 aatggaggtt gtgaccgagg agttggcggg agaacaccgc ttgttcggga taagggactg
     1381 tgacaagact tttgtcacag cctttttttt gtacaaaaaa gaaatttaca cctaatagaa
     1441 aaatttttcc ataaaaatac atattttaaa tatgaaagta caattggaaa tagattaaaa
     1501 cgataaaata aaattgtcaa aacgtcagag aggatggtgg agaatgaatc aatgtgtaaa
     1561 aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg atggtgacat ccgtttttgc
     1621 cgatgtagag aaaacaaacg aatcaacgca atcagagaat caaagttttg acaagactac
     1681 tttttcagaa gaatcgatta cttctgaaac tacagaatct caaagtgagg cttctactaa
     1741 taaaaggcaa gaagaaaata agacaagtga gacctcagaa gtagctgaac aaacgtctga
     1801 tagcagtgaa gcggcactgg caagagctgt ttcagcggat gggtacagcg aagctgccac
     1861 aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta actaagatcc gactgattca
     1921 gcctttgact ttagacaggg aattagagat caaacgagat atcgttattg attttggtgg
     1981 attcgctcac aatttcggca cacaccatat ttatatcaat gagatacctc agtcgatcga
     2041 gttccagaat tttaaaggaa cagctgcaca tccaggtgga ttgacaccaa atatcgatgg
     2101 aaatgccatt atcattgctt atatgaccga tttttgggga agcagatatc attttacggg
     2161 agaaataaaa tttaccggga cacttgattt gtatgatggg tccaaattag gactgattta
     2221 cgctcctcgt gcaactgtca cattagatgg tgtttccggt gtgttggatg tccaaccagt
     2281 gaaggaagga ctgaatgccg ctccgggtaa agcctatttt tgtagaagct atcaattgaa
     2341 tgtgatcaat ggtagtcaac tttatggacc gtatctaggg aaattctacg gttttctaga
     2401 ttcaggagat gagactggta gtggaaaaac agcgccagga atccatatct taagcggttc
     2461 aaaggtctct cttgattatg atcgggcaga tggcgttagc gatgaaggag aggcagtcga
     2521 tacgctacct ggtaatgtca tgtttgaagt cagtggttcg ggaagcgaat tttcggtaaa
     2581 tacaaagatc aatattacca atgacaacaa tcgcggaatc atccaaatga gaggttcagg
     2641 atcgaaagtc actgtatcga atggtgggaa aatcaccatc aatacagcaa ctacgggcgg
     2701 atttcgcttg caaggagatt ctagtcaaat ccatgtatca agtggtggac aaatctcggt
     2761 caaaatggct ggagatgacg atcaacccgg gaacaatggg atgcgttttg taggaaaagg
     2821 cctttctttg atcgtagaag gagccggatc agttatcgac atcgataagc agagtgggcg
     2881 ttccagcgca gttcgttttg agaatggtac gcaaaagctg acagtaacca atggaggctc
     2941 attgagggta agaaatacag gtgatggcaa atcttacgtg aacagactca atcaaggaaa
     3001 tcaagcgatc caattttatg acgcttctgg ttttctagaa agtccaggtt cagctgattt
     3061 tacagtcact ggagaaaaat caaatatcga tatacgcgca gattccggag caactgtaga
     3121 tacaactgga gatattgatt taaattttac ggcaggtgaa aaaacttatt tagtcatggt
     3181 cgggaaaaca ggggaagata agaccggcat catcagtggg gatgtgttga atgtggactt
     3241 agaatctcct acctattttg atttccaaaa caaacggatt ggaagcgaga ccaatggcat
     3301 aggcggctgg gtattccaag gaaatggaga ttcttcttta aagatcgccc attcagaaat
     3361 cgctttatgg cgaaaaacag gcgcaacatt cgataacttt gaaaaagacc cggattattt
     3421 ttctgatatg acagacttat cgatcaatgg gaccgactta ggcaatttcg tatcaagccc
     3481 tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa attataatcg
     3541 aatcagtgcc aataatcagc tcccgatgat tgaatcctta agagtgccaa cgaatgcaga
     3601 taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc caagagatgc
     3661 ttggacaggc gaagtagaag tgactttacg tattacttac gcagatacga cgaaatcatc
     3721 tgtagaaatt actgctctca cgcaaggaca aacagatgaa aatgaagggg caggatataa
     3781 cccatgggga gaaggcgaac aaggcggctt gttcgaagtt caagtaccca atgatgagag
     3841 attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa aaagtaagag gtggccaaga
     3901 agtgacagaa ctggactctg ccaaaacgac agtcgatgtg attcctcctg aacctgctaa
     3961 tttaagtacc acattgatca atgcagcttc tcgcacgatc acaggtacag gtgaagcagg
     4021 agccttggtt tcattgaaaa aaaatcaagc tgttttgtca gaaacaacta ttgatgatac
     4081 aggggaattt gttctttcta tcccagaaaa tcaacttttt gcaggagatg tattagaaat
     4141 tgtattaaac gaccaagcag gagaagctac tgctaagggt gtgcagaaca agccaagtac
     4201 caacgatgaa attgggaatc acaatccaag tgatacgcca gttgtttatc atgatgcccc
     4261 gccttttgca cctgctacga agatcgtagt agaaggtggt ctctcatttc ttgccccttc
     4321 caaactcgat tttggtcaga tcaaagcaac tggtttgaca caagaagcat ttggccaaat
     4381 ggatatacag gaaaaattat ggatttatga tcagcgagaa acgaaaaatc cgtggatctt
     4441 aacgataaaa ttatctcagc ctttcacaaa tgatgcagga ttttctatgc aggatgtcct
     4501 atatttcaag aaaggagagg agtatcaatt agtgaatgaa gaagaactgg aaattgtcag
     4561 aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa tatttaaata acggtcgggc
     4621 gtttaaactt gtcttaacca aagaaaatca gatagctggt aattactcag cagagataac
     4681 atggacatta gcagatgcgc cgacagaata aagatcaagg aggatgattg atgaatcgaa
     4741 tatggaataa aagtcatcga aacaactgct ctttacttat gatccttctc cttggattca
     4801 tcctttggca gacacctgtc catgcctccg aattcaattt tgcggtagat tttgaattac
     4861 cggctaatca gattgataag aacaaaacat atataaatgt aaggatggag cctgggaaaa
     4921 cacaggaaat cccctacacg gtctttaacc gaacagataa atcagtaaca gttgagacat
     4981 tgatccactc cgctacgacg aattcaaacg gtgtgatcga atatggagaa tcaaaaagta
     5041 aatttgatga aacattaagt tatgatatga aaaacttagc aaaaacagtg ggagaaataa
     5101 ctgttcctcc tcaaagtgcg gtcactgaga cactttcact gaaagcgcca gaaaaagagc
     5161 tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa aaaagagaac gtttctaaca
     5221 aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa agcaatcttg atttgaaata
     5281 gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc ttatgcggat caaagcgatg
     5341 gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc ttcttatatg ttcggcttga
     5401 ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc ttacctttca ataaaaaaag
     5461 aaaaaataga tgtagcaccc aatacaaatt ttgatttact tgtcccatta gaaggaaaaa
     5521 aattagctcc gggacattat caagcggttg tgaagggtgc ttggaaagaa aaacgctgga
     5581 atctccatac cgattttgaa ataactgatg atgcagcgga ccaattgaat gaaaaggata
     5641 ttgatttaaa aaatcagcca gaaacatttg actggcgatg gctgatactt gttggagtca
     5701 ttcttattgg cttatttgct ggattattta ttttaaaaga aagaaggaaa aagaaatgaa
     5761 aaaaacaatt gcattaagta ccgttctagc tggaagtcta ttattatgta cacaaagtgt
     5821 ttttgctgag aataagagct cagccactac agaaggaacg atttctgtga ccaaaccaga
     5881 tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt gatccaaatg atcctgatac
     5941 tccttttaca cctgacgaac caaatcaggg aacatccgga ttactgacga ttgaccaagc
     6001 tccagatctt gattttggaa caatcaagtt aggtacagca aaaacagtat atgctgcatt
     6061 acaaagcgga acggacagta ctggagcaca aacacaagta cagaatttcg tacaagtaac
     6121 ggataaaagc ggaaattatg ccggttggaa attatcagtc aaacgaacag aatttattgg
     6181 tacaacatct gatacgaata aactaactgg ttcaaaggtg aaattcaaaa acgccgtgat
     6241 tcgaaatagc gcaactaata gcagtggaat gccatctacc gtttctggaa ctggcgctga
     6301 aggaatcgaa atcccagcag atcaggcagt agatctagta actgcaggag caaatgaagg
     6361 gattggcaca tggatttatt cattaggaga gaatgtggaa caagggagac aatcagtgga
     6421 attatttgtt cctgtatcca attatgctgt agacaactac gtttcaacat tcacatggtc
     6481 gttaacagat gctcctacag aagaggaagc gtaaaatcat atcaacgatt tcagggagtg
     6541 gagccgattc aaggcccact ccctgaaatc tgctatgctt acgtctcagc tcttattcat
     6601 caactcgaag cagttttttg gaagtcatgg aaacgttcct ttgtcttttc aaagatcttt
     6661 cgaccaacga gtaaggagtc accgttgaaa aattctaaca cataatgagc agaatcaatt
     6721 ttctgcaatt gcgataagtt gatacagtaa gattttaaaa gtaaaaagta agcatacttt
     6781 tctaactctg tcttcaattg ttttaaagaa gtattcacta atttatcttc tgtagttgtt
     6841 ttgaccataa cttggttttt caaacctttg atactttcaa tatataaaat ttcccgagga
     6901 tacaggacga gacttccacc tttttgatca ataaagattt tatcgacatt tacgatctgc
     6961 tgttctttcc aattgatctg ttctagcact tgacgaattt gttcagataa attcctgtct
     7021 tttctgatat atccaatcgg aaggattttc cgattgatga tctctactgc aaaattcgta
     7081 taagcggtga aaaaaacaat attactatgt gcattttttt tacgtatttg ttcggcgaca
     7141 tcaatacctg tgagtgattg cttcaagtcg atatcgataa agaaaacatc gtgattatca
     7201 atagtgccca actggatcaa ctgattgatt tgagagatag aagtgagctg cacgatattc
     7261 tcgatatgat gattgaccaa ttctctgatg atccattcgc tgtgaaactg gttatcttct
     7321 atgataaaat actttttcat atgcaggctc ctattaaaaa aatatgtgta cattttgaaa
     7381 caaaacaatg gaggaataag atggtcgcaa cagtgacact ttatttgacc tttactttga
     7441 ttttagattt aggtatctat ttattctttt cgaaacaact gctagtaaaa aacagctttg
     7501 gttgtatagc tttgatggtg gtgacacttt tacttcctat ccctaaagtg agctttcctt
     7561 acctagttgg cttattattg atgtttcttt tttattttac tacgaaaaac ctgtttatca
     7621 gctgtttgat cagtaatctt ttttttgcta tcactggact cagctttttt ctggtttttg
     7681 atctgatgga ttatttagga aagagacatc ttggaacagc aagttttttt gcactactta
     7741 tcgcatttat tttctatcta ttagtaaaaa gtatagacaa acagacgaga tttctcaact
     7801 tgctttttac ttatccaact gaacactatt ttggaggtat ttgtatttta ttaagctggg
     7861 gcgtgattta cttggtattg ggaagtgttc acttccgctc atctaattat ctgcttctat
     7921 ctcttttgtc gatagtcatc agtttttttt ctttgttttt ttccattatg ttgatcagtt
     7981 tccaagtaaa agaaagacaa catcaggaat cgcttcaact gtatcaaata aatgaagaat
     8041 attaccatca tctagaggaa ttcaaacatg attataaaag tctactgttt tcattaaaaa
     8101 ttactcttca ggaaaataaa aaagaaacgt tggaattttt agaaatatta gaagatcatt
     8161 ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac aaatattgta tccccagctg
     8221 tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc ggagaaaatc ccgatccaag
     8281 tagttattcc tgtcgaagtg aaaacaatcc cgattgatct ggttgttttt attcgctgtt
     8341 tatctatttt tatttctaac gcattcgatc atcgtgtagc cgatcagagt ccgattacga
     8401 tcaatctgct ggaagaaaaa gagggatcac gttttagtat agcgaacaag gcaaatccaa
     8461 cgaatttgac gctatcggag atggtcaaaa gaggaaaaac ctctaaaaaa aatggcgggt
     8521 taggtctata cagtgcgaaa aaaatgttgg atgtttacga aaatgcagaa ctaaaaatcg
     8581 aattttcaaa acagaatcac tatttttttg tagaaatgta tctggcatct agaaagagca
     8641 aatcagccta ctgacttact gtcaaaggat aagtttgagg aaagtctaca cggagataga
     8701 gacaacaaca cagcatattc aaaagaaaca gaagcgtgat gaccgttgtc acccttctgt
     8761 ttcttttgct atgtagttaa acaaagtagt tgacacttcc gctacttagt attagtacga
     8821 aagtatactt catgatacta agtagataag aagtgaaaag cggaacccac gatcgtcaaa
     8881 atgctaactg ctttgcttac accagataaa gggaagagca atatcgctta ttttaggcgt
     8941 ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct gacccagcag ttaagagagt
     9001 tagaacaaga tgaaagtatc catcgaaaag cctataacca agtcccgcct aaagtagagt
     9061 actcgctcac cgaagaagga aaatcattaa gagagattct agtagcgttg tctgtttggg
     9121 aagaagaacg tatcgaaaaa caaaaagcct caagggaaga agacacgcta ctgcatacac
     9181 atggtggcta cctaaattat tgattttcta tgacatttat tttaaataaa agaaaacagg
     9241 agttttttta aaagcatcat ctatttttta tgtaaaaaaa tggtatgatt aatcaagtaa
     9301 gaagtgattg gaagaaggca agcaaatgaa tagtatggaa aaagtcaacc agtttagaga
     9361 tgaacgtaat tggcgacaat ttcataacga aaaagatttg gctatctcta tttctttgga
     9421 agcatctgaa ttgttagaac tttttcaatg gaaaacgccg gaagaagtaa aagaaacaca
     9481 attagaacga atcaaagaag aactggcaga tgtattgatc tattcataca tgatggcaga
     9541 caaccttcac ctggatctag atgagatcat tgaagaaaaa ttgatcaaaa ataatttgaa
     9601 atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac acagagttgt agaaatgtgt
     9661 cattcggctg ttggaaagag caaaagaaaa acagtaaaat gagtctggag ggagagaaaa
     9721 tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat cctcttgttc ctcttgttat
     9781 tagtaggact agctttgatt ttcaatgaac agatcaagga ttattttgtc agagagacag
     9841 gagataaata tgctatagcg aatgtaacaa aagaagatct gaaaaagaat aacgataaag
     9901 atgtcagttt tgactttgat gcggtagaac caatgacgac agaaggggtc atgaggtcgc
     9961 aaatgagagg aacagatcta ccagtgattg ctagtatcgc agtcccttca gtttcaatca
    10021 atttaccggt tttcaaaggc ttggataata cgtcactact ttacggagca ggaacattat
    10081 ctccagatca agaaatggga aaaggaaatt atgccttagc aagccatcgt gcaacgaacc
    10141 ctgaattgct gtttacaccg ctagaaaatt tagagatggg tgccaagatc tatttgacag
    10201 atttagaaaa tgtgtataca tacaaaacat tttttaaaga aaaagttgct cctacagata
    10261 cccaattact gaatgaagtc gagggaaaag aaattgtcac actaattact tgtggcgaca
    10321 tggacgccgt cacgcgacta gttgtacaag gagaactgga aagtgttaca tcgataaaag
    10381 atgcgacaga tgatatgaga tctgctttca atctagagac aaaaacattt taggatactg
    10441 ggacataagt cgttacagat taaaaacagc caaaggatat gaatcgataa ggaaaaacca
    10501 atttgctgaa aaaaagagaa acgcaaagag gtgcatctct gatttggttg ttttctagat
    10561 agacttgtgt tccagttttt ttatttttta tatgaaaagg cttgcataaa taaaaattcg
    10621 atggtatgat gattttgaat aagaaatgga ttgttactgt acggcaggca aaaccagaat
    10681 tcagctaaga aacagtaggc gaattctagg tttttttatt tttcttgaga gggcgaagca
    10741 aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat ttcgaaaaat ggattcgggg
    10801 aagaagtcgt ttgtatggga aaaggactag cttttcaaaa aaagattggg gacgagattt
    10861 cgccagaagc ggtccaaaaa gaatttgttt taagagattc ttttgctaaa aaccaattcc
    10921 agcagctgat ggctgatatt ccggcagagg agatcgaatg ggtcaaacaa gtagtcgaac
    10981 tggctgaaga taaactaaaa gttgaatttt cgcctaatat ttatctgaca ttgaccgatc
    11041 atcttcatta tgcgatcaca cgagcaaaag aaaatatcca actgcctaat ccattattat
    11101 ttgaaacaaa aaaattttat ccgaaagaat atcaagcagc gaaagaggct cttttgttcg
    11161 tgaaacaaaa gacgggtgtg gaactttctg aggatgaagc cggttttatt gcctttcatt
    11221 ttgtgaatag ccaacaagga aacgaagaca tgcaagtgac gatgaccgct accacaatgg
    11281 taagagatat actgagtatc atcagtaaat tcttcggcat cgtgttcgat gaagaatctt
    11341 tgaattatca gcgtatcatt actcatctgc agttttttgc ccagcgttat ctcaaagggg
    11401 aaggatctga cgagcaagat gaatttctct acgcattagt tcaaggaaag tatccaaaag
    11461 cgtttcattg tgtcgaacgg atcaatgagt atcttcgaca aacgcaaaaa caggaaatgg
    11521 ggatcgcaga gcagatttat ttaacgattc atattcaaag agtagtaagt gagaaaaaaa
    11581 gcatccaatc ataaggattg ttactgtatg gcaggcaaaa cctggattga aaaaataggt
    11641 tcacgaatgt ttgtgactgt ttttttcaat ctgggttttt attttttaaa taaaaaagaa
    11701 agaaggaaaa aacaatggat aatcaagcag ttggtcgtcg tgtatgggaa gcggtaggag
    11761 gacaaaagaa tgtcaaaagt ttagtacatt gtgctacgcg gttgcgattc agattaaaag
    11821 atgaatcttt agcagatacg caaaagttaa aagaagaccc ggacgtgatc caagtcgtac
    11881 aaagtggcgg tcagtatcaa gtagtgattg gcagtaatgt agcagatgtt tatcaatcga
    11941 tcgtggatga ggaaggtctt gcagtttcgg aggatggaaa agaagagtcc aaaaatattt
    12001 tgaatcgtct gatcgatatt atctctagta tatttacacc ttttctaggg gcgatggcag
    12061 ccgccggggt attgaaagga ttcttatcat tggcaacggt gatgggctgg ctgacagctg
    12121 atagcggtac ttatcaaata ttgtttgctg cagcagatgg tgtctttacc ttcttacctg
    12181 tcatgcttgc ttttacagca gctaaaaagt tcaaagccaa ccagttttta gcagtagcaa
    12241 ttgctatggc acttgtgtat ccagctatca cagcagttgc tggtgcggga gaagctatca
    12301 gtttctttgg gatcccagtg attctttcac catctggtta tacttcttct gtcataccaa
    12361 tcattctagc cgtatgggtt caaagtaaat tagagccatt cgtaaaaaaa gtgattccgc
    12421 aatttctgca aatgatttta gttccgcttg tcgttttagt agtgatggtt ccattaacat
    12481 ttcttgcttt aggaccaatc gggacggttg ccggtaatgc tttaggcggt ctcttcaact
    12541 cgatttatgg atttagtcca atcgtagcag gtctaatcat gggaagttta tggcaagtat
    12601 tcgtgatgtt cggcatgcat tggggattcg taccgatcat gtttttgaat atcgaacaat
    12661 atgggtttga tgtattgatg ccaatgcttc ttccagccat cttggcacaa ggtggggctg
    12721 ctttagctgt agcattgcga acaaaagata cgaaacttcg agcattaggg atttcttcta
    12781 cagtgacttc gttatttgga atcactgaac caactgtgta tggtgtgaca ttacctttga
    12841 agaaaccatt cattgccgct tgtatttctg gaggtattgg tggagctatt atcggatttt
    12901 ccggagtaaa agctttctcc agcagtttgg tcagcttatt gacgattccg actttcatta
    12961 atacggtgga tggtgtagag tcaaatgtga cagtagcagt aatagctaca ggaattgctt
    13021 ttgttcttgc attcgtaggt acattgattt taggatttga cgaacaaaca caagacaatc
    13081 aattagaaaa caaacatgcg aatgcaggaa aaccgattac ttcagcacgg cataccttaa
    13141 aaagtccttt aacgggtaaa gtactgccat tgagtgaagt accagaccaa gttttttctt
    13201 ctggcgtgat gggaaaaggt atcgctattg atccagaagt aggagaacta gttgcacctg
    13261 cagacggaga gatcacgact atttttccaa ctggacatgc tgtagggatc acaacgactg
    13321 acggagcgga gattttgatt catatcggga tggatacagt agaattaaat gggaatggct
    13381 ttgagatctt agtcaaacaa ggggatctcg tcaaagcagg agatttattg attcgttttg
    13441 acatcgaagc aattagagca gctggctata gcgtaatcac tccagttgtc attacgaata
    13501 ctgatgcatt tgccgatata cttgaattag accaaaaaga gattattgca aacgaagatg
    13561 ttctggcaat tgtaaaataa ggaggaaatc aggatgcaat cacgtttttc agaaaaattt
    13621 ttatggggtg gagcaactgc tgccaaatca attagaaggg gcttatgatc gagacggaaa
    13681 aggactatcc gttgcagatg ctatgcctgg aggtaagcag cgctttgcga ttatcggcag
    13741 tgaagaattt gactggacga ttgatcaaga aaaatatatt tacccgaatc atcgaggaat
    13801 cgatcattac gatcgtttca aagaagatct tgccttgttt gcggaaatgg gattcaaatg
    13861 ttatcgtttt tcgattgcct ggacacgaat ttttccaaat ggagacgaag gtactcctaa
    13921 cgaagcggga ttagaatttt atgatcagct gattgatgag tgcttaaaat atgatattga
    13981 acctgtgatc acgatttctc attacgaaat gcctcttcat ttggcaaaag aatacggtgg
    14041 ctggaaaaat cgaaaactga tcgacttcta tgaacgattt gctcagacag tattagagag
    14101 atacagcagt aaagtcaaat attggatgac tttcaatgaa atcaattcgg cctttcactt
    14161 tccagcttta agtcaaggat tagtaaaaag caatggagca ggagagtacc aaaatatttt
    14221 ccaagcatgg cataatcaat tcgttgcaag tagcaaagcc gtaaaaatcg gccacgaatt
    14281 gcgttcggat atccaaatcg gctgcatgat tatctatgca acaacttaca gcatcgatgc
    14341 caatccagtc aatcaggcag caacaatgat ccaaaaccaa gaattcaatt ttttctgcac
    14401 ggatgtccaa gtacgtggag aataccctgc atacacagcg cgtacccata aaaaatatgg
    14461 agtcgatccg gaaaaattag agcaaacgga aaaagacttc cagttgctaa aagaatatcc
    14521 agtggactac atcggattta gttattatat gtctacggca atcaatgaga ctgatccaca
    14581 agcagcaact tcagaaggga acttgctggg aggagtaaaa aatccgtttc ttgaggcaag
    14641 tgaatggggc tggcagatcg atccagaagg gctgagaatc gcattgaatg aattatacaa
    14701 tcgttaccag aaaccattat tcattgttga aaacggtctt ggagccattg atcatatgga
    14761 agaggatggc acgattattg atgattatcg gatcgattat ttacgtcgtc atatcgaagc
    14821 gatggctaat gcagtagcag acggtgtaga tttgatgggc tatacgcctt ggggatgtat
    14881 cgatcttgtc agtgcatcaa caggagaaat gagtaaacgt tacggcttta tctatgtgga
    14941 tctagacgat gaaggaaatg ggacactgaa tcgatcaaag aaaaaatctt ttgattggta
    15001 taaagaagta attgcctcaa atggcgtaaa cttgtaatta aagcagctga atgaaaaaga
    15061 acacacttct cacatgtgat ttgtgttctt tttttatttt ttatgtgcta ttcttttagt
    15121 aatagtaaag caaagggtga cgttatgaaa aggacacgtg tactatactt ggaagtcgca
    15181 gataaaatta aagaagatat tttctcagga aaatatccag tcggttcgat gttgccgaca
    15241 gaaagtgaat tagaagaact gtttcaagtc agcaaaatca ctgtccgaaa ggcaatcgaa
    15301 ctgctggcat cagatgaata tgtcgcaaag aaaagtggtc gtgggacgac ggttctaagc
    15361 aatcgtcctt acaatcgatt atccaaagcg gcaagcttta cgcaaatctt ggagaactcc
    15421 cgatatgaag tccggaaaga aacgctggat ttgcaattgg tccatataaa gaaggaagat
    15481 cctttgtttt cctttttcgg tgagcaagct gtttgtttta agcgcttata ttatttgaac
    15541 aatcagcctt atatttattt tgttcattat cttccacaag aaatgatgca attcagcaga
    15601 gaagaatttg gcaaagaatc tttgtatcgt ttgctgacga aaaaaggata tactattgac
    15661 tcattttctg atgatttctc ggcagtattt ttaacagaag aagaacaagg aattctgaaa
    15721 acagacgcac agatcggcat caaacggata cggaaatcgt ctacgcaaat tggggaaatg
    15781 attgaatatt cggaggcggt ctaccatacg gagctgcatc cttaccatat tgattatgaa
    15841 acataaataa aaaaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca
    15901 aacaaaaata gcttctcata tttttcgttt ggttaggctt attaccgcag atacacggct
    15961 gtttctggat taccatttat tctggataag ggggtgtggc acgattttca gaaggaatct
    16021 atgaaccaat cactgtattg attttagctt cgatggtaac cccattcggc ttgacgatcg
    16081 cttatttctt agggaaaatc attcgtaaaa atatactcaa tcgtcaagaa atcgatacat
    16141 tgaagaccgc ttttcctatg gggatctgtc agatcacaga gggatgtttc cctatcgtat
    16201 tgaacgactt ggcacgcaat gtcatagcaa cgggagtcgg tgggacagta ggtggcggat
    16261 taagcatgtt ttggggcgca gatagccgta ttccggcatc gggaatgttt gcagtcgcta
    16321 cgatgaccag accatgggca ttcattggtg cattgttagc tggttctttt gtcacaggaa
    16381 taaccatttt attattgaaa aaaccagtag atccgaatgc agaaatcatc caagaagaaa
    16441 aagaggaaga agacatatct tgggatgatt tgacaattag ttaggagagg cagaatggat
    16501 tacaagaaaa aagtacaata gatgtacctt gagacaggaa tggtatgaaa gaatcagagt
    16561 tagaaagaat cgaatataca gattttggtt taggagcgat cgaaactgtc cagagcatcg
    16621 gcaccctcct tgaaatgacg atgaaggaaa aatgagaacc tttcgttgtc gaaagggtct
    16681 ggtatacctt atgtcgaagg aaaggaaaca aaaaaacttt cctgtactat tccaaaaagg
    16741 aaagaaagtt attatacagt aaaaaaagaa attattcttc atgctagaga tcagtacacg
    16801 ttcgagccca atatcaaaca ctagttccag gcaggcgcaa caggtgcggt tatcactgaa
    16861 ttttcttcca gcagtgacga ttcaagtgat atttttacaa atccagctat ttatcgttaa
    16921 caaaaagatt tttaggagaa gctgatgaac aaaaatgatc aaattttgat cgtttagttc
    16981 gtcggctttt ttatttagag aaacatcatt tatcagtgaa acataacacg agaacaaaat
    17041 gattttttcg ttaataaaag aaacaagtat
//
LOCUS       DACDKL010000045        16898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDKL010000045 DACDKL010000000
VERSION     DACDKL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.045"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(15..911)
                     /locus_tag="IV329_001137"
     CDS             complement(15..911)
                     /locus_tag="IV329_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8940334.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(1192..1869)
                     /locus_tag="IV329_001138"
     CDS             complement(1192..1869)
                     /locus_tag="IV329_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8940335.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(1960..2865)
                     /locus_tag="IV329_001139"
     CDS             complement(1960..2865)
                     /locus_tag="IV329_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8940336.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            3040..4494
                     /locus_tag="IV329_001140"
     CDS             3040..4494
                     /locus_tag="IV329_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8940337.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            4510..5541
                     /locus_tag="IV329_001141"
     CDS             4510..5541
                     /locus_tag="IV329_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8940338.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            5548..6735
                     /locus_tag="IV329_001142"
     CDS             5548..6735
                     /locus_tag="IV329_001142"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8940339.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            6891..8273
                     /locus_tag="IV329_001143"
     CDS             6891..8273
                     /locus_tag="IV329_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8940340.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            8245..8631
                     /locus_tag="IV329_001144"
     CDS             8245..8631
                     /locus_tag="IV329_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940341.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(8831..9499)
                     /locus_tag="IV329_001145"
     CDS             complement(8831..9499)
                     /locus_tag="IV329_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8940342.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(9688..11114)
                     /locus_tag="IV329_001146"
     CDS             complement(join(9688..10541,10541..11114))
                     /locus_tag="IV329_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8940343.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            11357..11812
                     /gene="tnpA"
                     /locus_tag="IV329_001147"
     CDS             11357..11812
                     /gene="tnpA"
                     /locus_tag="IV329_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8940344.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            12077..12175
                     /locus_tag="IV329_001148"
                     /pseudo
     CDS             12077..12175
                     /locus_tag="IV329_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12398..12628)
                     /locus_tag="IV329_001149"
     CDS             complement(12398..12628)
                     /locus_tag="IV329_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8940345.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(12858..13676)
                     /locus_tag="IV329_001150"
     CDS             complement(12858..13676)
                     /locus_tag="IV329_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8940346.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            13837..14526
                     /locus_tag="IV329_001151"
     CDS             13837..14526
                     /locus_tag="IV329_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8940347.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            14540..16042
                     /locus_tag="IV329_001152"
     CDS             14540..16042
                     /locus_tag="IV329_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8940348.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            16055..16531
                     /locus_tag="IV329_001153"
     CDS             16055..16531
                     /locus_tag="IV329_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8940349.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(16628..16738)
                     /locus_tag="IV329_001154"
                     /pseudo
     CDS             complement(16628..16738)
                     /locus_tag="IV329_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5177 a         3066 c         3189 g         5466 t
ORIGIN      
        1 ttttattgct tttatcaatt caattttagc gtctcaccgg gagtcaggaa ataattctcc
       61 atgctcatcc cattcagcga caagatcgtt tctactggta cgccagttcg ttcggctatt
      121 tgatttggac cttctccttc caaaactacg gttgtcgcag cctgtgaagt cgcgctgttg
      181 gtattagttt cagaggaatc tgttgtgtct tgtgttgttc gagcttgcat ttcagaagct
      241 tctaccgact ctgctgtctc agaatcagaa gatgttgtgt cttgtgtgct tgttgactct
      301 gcttgaagag aggcgctagt ctgcaaataa atatcggaaa tcttagcgac atgattcgta
      361 taattagccg ataattgatt gatcaaagta tctaagtcta caccgtcttt attttctgct
      421 gcctgatacg ctgcacgtaa ttttctgatc ccttcgtcgg agattgcttg aagctcacgt
      481 gctttttggt tagcaactgc tgataaacca gctattccct cttgatttcc ctgaatttct
      541 gtctggtatt cttcaatcaa tgcaggcgtc ttttcttcga tctttttcga ataatcattt
      601 acgattgctt gaagttgctc tggcaaacca tttgctgttt tttcttcaga agtttgtgtc
      661 tcttttgttt tagttgatga ctcacttgtt tgtttcgtct ctgttttctt tgtcgttgtt
      721 tccgttatgg tcgtactttt cgcttctgac gaattttttc catgaaaata tagacccgca
      781 actcctcctg ctatgatcaa aaacacggca actgctgccc caatcatgat catccgctta
      841 cgagctgcct gtttttgctg tcttcttcgg gtcatccgaa cattttctcc ccactcattt
      901 tcatcataca tttttttcac caccaatttt tatgtataat agcgttatca aacgcttcat
      961 attcttattg tagcaaataa ttgtcgctgt gcacattttt atctttgtta tttataagaa
     1021 aatttcacca gttgagatga ttccaagatt gtccgtcgat ttctccttgc agctgcattg
     1081 ttcgttcgtc tatctcagcc atcagtttct ttatttcata taagagcgca cggtcttgat
     1141 attctgattt ttcgcttaat tcatctaatt gttggatcag ccaatcattt gtcataatga
     1201 agttcctttc atttcttgca gtgtaaaggt caagtcttcg atctgctctt ttgcctcagt
     1261 cagtgcttct acgatatatg cttgtttttc agattcttct tcctgcattt tctgattttc
     1321 ctgtactctt tgaataaacc agtcaatccg ctgagtcagc gatggtttgc cttccaatga
     1381 gagatactgc tgataagcaa ttagcttctc ccaacgtttt tcctgggata aatacataaa
     1441 ttgtgctaaa acgaatggcg gaagataggt catatggaat ttatttgcga ttcgctgata
     1501 cggcctcaaa aattcggaaa tcgtatattc ttcttttcct ccagcttggt attctttttc
     1561 cgccacacct gttgtcaata cgattcctag ttctttccct tttaatacat gtgctgctcg
     1621 ttccaagaca ttatcttgcc aaatttttaa atgagcagga gaactatacc agtaaaacgg
     1681 aaactggaaa atgatccgat catgctgggc caagagctgt agttcagtct ctatatctat
     1741 ttttccgtct ggataacttg attccaaatg atgccaagta gctggtaaac cagcagaagc
     1801 ttctttgaaa aattgctgat ttaaagattc ttcgatcgtt ggatggctaa tgatgataag
     1861 tgtacgcatc ccttttcctc ctttttagtc ttcgctttac agtgtagcat aaccgccaca
     1921 agtttctttt gccgatgctt gtcattttaa ccagactaat caggttgtcc aagaaactgc
     1981 ggtttgtttt tccaactgtt ttttcaaggt atccaaagca atattcttaa aattttcgtt
     2041 tttcatacgt ttacgatagt tcgtagccgt ttctccggta ctctttttga acattcggct
     2101 aaatacgact agactattga accctacttg ataggcaata ctttccacgg attcatccgt
     2161 cagagaaaga agaaacgccc ctcgattgat ccgtaccccg atcaaatatt ccttgatact
     2221 ggtacccata tgatttttga atactcgcga caaatggctc cgattgaccg ctaaatgctg
     2281 ggcgatttcc tcaacagaca atttttcagc ataatgtgcc tcgatgtatt tgactgtttc
     2341 aacagctaaa gaggaatgaa ccttttgtac accaccgctg acctttccgc catcttttaa
     2401 aaggactgat aaaaaacggt aggttaatgc tgttaactgt aattcatttt ccaaggtttc
     2461 ttccgtatct ttcatgcatt ctaaaataat gtcgatatat ttttgtatgt cagaagagac
     2521 catcgtgtat tggtcttctc tgaaaagcga ctgccgaatg atttcttctg cagcactccc
     2581 accaaatgaa agccagcaat acatccacgg atcttctcca tctgataagt aaaacgtcga
     2641 gtctcctgga cgaatcaaga acagatcacc ttttttcaat tggaattgtt gatttttcac
     2701 atgataagat cctttgcctt ccatcacgac atgcaggatg taatcattgc gaatagcagg
     2761 accaaaagaa tgaaacggca tcgttttaga gaaaccacaa aaatcaaaat acaattcttt
     2821 ttctacttgt tgatgttcct tttttaaaaa aatcgattgc tccacgtcta tccctcctta
     2881 ctcctttagt ataatgcaac aaaaagttaa ataaaagtca cagaaatata ttaaaaagga
     2941 agcgatttct tgttaagttt aagtcattaa aagatctttg ttattcgttg taaccggttt
     3001 acttagtatt atatattgaa caggagagga atacagatta tgaaaagcaa aatgatccaa
     3061 cgtgtgtcct acgctttagg cgcttttgga cacgacacat tctatgttac attaagcact
     3121 tattttatgg tattcgtgac aagccagatg ttcgcaggaa cagataaggc aacgaatgca
     3181 aaaatgattg ctacagttac aagcttagtt gtaggtatcc gcttagttga aattattttt
     3241 gatcctttga ttggtggcat catcgataat actcgtacaa aatacggtaa attcaaacca
     3301 tggttagtta tcggcggcgt tgttagttcc gtactcttgg tagcaatctt taccaatttc
     3361 tttggcttag ctactcataa tacgacttta tttatcatat tatttgcaat cgttttcatc
     3421 ttattggata gtttttattc atttaaggat atcgcttttt ggtcaatgat tccagctttg
     3481 agtacggaaa caaaggaacg tgaaaaattt gctacgtttg ctcgtttcgg ctcttctcta
     3541 ggtgcaaatg gtactactct agtcgttgtc cctatcgtta ctttctttac ctatcttgtt
     3601 actcataaac atattgaagg cgcttcaggc tggttctggt tcggtgtgat tgctgctgtt
     3661 atttcaggcg gtactgcgat tatcactgcc ttgggcacaa aagaagtcga tactgcgatc
     3721 cgtcagcaaa atgaaaaagc tgggatcaaa gatgtgttca aagcagttgc tcaaaatgat
     3781 cagttgatgt ggcttgccct tgcttatatc gcttatgcga ttgccaatgt agcaacaact
     3841 gccgtattat tctacttctt caaatatgta cttggccaac caactgaatt ttggatcgtt
     3901 ggtctagttg ctgccttact tggtttagta gctgttccac tattcccaat gcttacacgc
     3961 gtcatctctc gtcgctacgt ttaccttgga gggatcgcaa tgatgatttc ttcttacttt
     4021 ttctttacga tcgcaggttc aagtttgcca atcgttatca tcgggctagt attgttctac
     4081 ttccctcaac aattgatttt cttatccgct ttgatgacga tcacagactc tgttgaatat
     4141 ggtcaatgga aaaatggtat ccgtaacgaa gctgttactc tatcaattcg ccctttacta
     4201 gataaaatcg ctggtgcttt gtctaacggg atcgtagggt ttgtcgctat cgcttctggg
     4261 atgactggta atgctactgc tgctgatatc actgctcatg gcgtaacgac tttcaaagct
     4321 tatgcattct atgctcctgc tgctttgatg attttctccg ctattatttt tgcttggaag
     4381 attacattga cagagaaaaa acatgcacaa atcgttgagg aattggaaaa caaattggct
     4441 ccagctgata caatggaaga tgaacttcac gatgcagtag ttccttccca ttaagaaagg
     4501 atcttgaaaa tgaagattac agaaaaagat tttggccaag gttatcactt gataaccttg
     4561 gagaacaaaa acaagctagc actttctatc tctgatttag gtgctagaat cgtcagttta
     4621 aaatcaaatg atcgagaatt agttttggga tttgacaccg ctgaagaata tatcgaaaaa
     4681 gatccgtata tcggtgcttc tatcggtcgc accgcaggga gaatcgaaaa tggccgcttt
     4741 tctctcaatg gaaaaactta ccaattagcc acagatccaa aaactggaca taatctgcat
     4801 ggtggtgctc ctggatttga attaaaaaaa tggtcttatg tcattttgaa tggcgaaaat
     4861 gaagcttctg tcatttttac tactacaagt cccgacggag aacatggttt tccaggtaca
     4921 atggatgtgg aaatccgcta tactttgaca aaagataata tctggcgagt gacctctcgc
     4981 ggcaccagtg accaagatac tttattcaat ccgacaaatc atgtttactt caacttgacg
     5041 ggagatgctt ctcagtcaat cgatcaacat gagctttggc tcaatagcga agcttatgct
     5101 cctttgcgaa cagatagtat tcctataggg gtaaaagaaa atgctgcagg aagtgctttc
     5161 gattttcaaa tacctaaaaa attagcgtcc gtttttgcta gtgatttgga tcaaaaaaat
     5221 cttgttgacg gaatcgatca tccatttttc ttaaaagaaa ccggcttggg taaagaagcc
     5281 gctcgcttga ctagccccga taaaagaatc caagtaggta ttgctacaga tgcttctagt
     5341 gtggttattt tcactgcaaa tttcggcaca gaaacaccag aaatgcgcaa ccgaaaactg
     5401 gcacatcacg gtggcatcac atttgagact caaacagctc caggtgcaga gcgcttctca
     5461 gcatttggtt cgattcattt aaaagctgga tcagtatttg aaacagtgac agaattcaaa
     5521 atcaaaacaa gaaaggaata actcatcatg gaaatcaaaa gaagcttaaa cacaaaattt
     5581 gccgaaacat tcggacctaa aaaaactaca caatacttct caccaggacg catcaatttg
     5641 attggtgaac acacagatta caatggaggc catgtcttcc ccgcttccat cacttatgga
     5701 acgcaaggtg tagcagcacc tcgagacgat aataaaatat tagtttattc cacaaacttt
     5761 gacgatatcg gtgtcattag ttttacttta gatgagctag aatatgataa aaaagcaaac
     5821 tgggcaaatt acgtaaaagg aatggtcctt aaattaaaag aagctggaca ctcaatcgat
     5881 catgggtttg agttgttagt ggaaggaaca atccctaatg gtgcaggact ttccagtagc
     5941 gcttcattag aattattagt aggtgttgta ttagaagatc tgtttgattt aaaaacagat
     6001 cgcttggaac tagtccaaac aggtaaaaaa gtagaaaatg aattcatcgg tgtcaattca
     6061 ggtatcatgg atcagtttgc catcggcttc ggtgagctag atcatgcaat cttattagat
     6121 acaaatacgc tgaaatacga aatggttcca gtgaaactag atggttatgc aatcgtcatc
     6181 atgaatacga ataagcggcg ggaattggca gattcaaaat acaatgaacg ccgtagcgaa
     6241 tgcgaagaag ccttggcacg tttgcaaaaa actttgccta tctcttcttt aggagactta
     6301 gatgaagaag aatttgaatc aactattgac caaatcggcg atgatacatt gatacgcaga
     6361 gctcgccatg ccgtttctga aaatcagcgg actttgaagg caaaagctga attagaagct
     6421 gggaacttag aagcatttgg tcaactgtta aatgattctc accactctct tcgctatgat
     6481 tatgaagtaa ctgggatcga attagataca ctagtagatg ctgctcaaaa acaagaaggt
     6541 gtcttaggtg cacgaatgac aggagctgga tttggtggct gtgcgattgc tcttgtcaaa
     6601 gaagaaaata tacctgcctt caaaaacaat gtctatgatg aatacatgaa agttgtcggc
     6661 tatgctccgg aattttatgt tgcacacatt ggaaatggga caacaaagat cgatccagaa
     6721 gctgttcatg tttgataaaa aaatcaacga tctcagctaa aaacaaatag ataaaaataa
     6781 cctctcagca atttccaaat gtgagccttt tgctcgtatt tgaaaattgc tgttttttat
     6841 tgacacggaa agagaacgtg tgttctaatt gtgttgaaga ggtgattgtt atgtgggcga
     6901 atcaaaatcc ggtatttgtt tatgaaaaag aaccttcaag agatatttta tgtattgact
     6961 gcaaatcttt ttacgcctcc gtagagtgcg ttgaaaagga tcttgatccc cttacaacta
     7021 aattagtagt catgtcctat ccgtccgatt cgacagaaac acggggtagc ggattaatct
     7081 tggcttccag tcctgcagca aaaaaagctt acggcatcac caatatcagc cgtgccagag
     7141 atcttccctt cccctatcca agtgatttat atattgttgc cccaaggatg gcctattata
     7201 tggaaaagaa cagagaaatc aattctatct ataaaaaata tgcagacgaa gcgaaccatc
     7261 atgtctattc cgtcgatgaa agcttccttg atgtaacaaa tagcatggca ctctttggta
     7321 aaacaaatgc gtacgacatg gctaaactga tccataaaga tgtgtttgag cagaccggaa
     7381 tctacacgac tataggaatt ggcgataacc cgctacttgc taaactagcc ttagacaatg
     7441 gctcaaaaga cggaaagcaa atgtttgctg aatggcgtta tgaagatgta ccagaaaagt
     7501 tatggaaaat ctcagatatc actgactttt ggggaatcgg caaacgaaca gcaaaacgtc
     7561 tgaaacaaat gggtatacat tctgtttatg atttagctca cgcggattat tatcacttga
     7621 aaagcaagct aggggtcatc ggtactcagc tttatgccca tgcttggggc atcgatcgaa
     7681 gttttttggg acaagaatat acaccaaaat caaaaagtat cggaaatagt caaatcctag
     7741 atagagatta tacaagacga gaagaaatcg agatcgttat caaagaaatg gccgaccaag
     7801 tcggcactcg cttgagacga tctgcagcaa aaacacaagt agtcagctta tgggtaggat
     7861 actctttagg ctatcttgat ccaaatggca agaccgggtt tcaccagcaa atgaaaatcg
     7921 aagcgacaaa tgctagcaga gatctagcag atgctttatt gaccattttc caccagcatt
     7981 accactacca ggatattcgg cgtgtaggcg tgaactgttc cgacctcgta tacgcgacag
     8041 gtttacagct taaccttttt gaagatcctg aaaagcagat aaatgaagcg aaagttgatt
     8101 ttgttgtcga tacgattaga aaaaaatacg gctttcaatc aatcatccat gcgcatagtc
     8161 tcttgcaagg aggacgcgcc atcgcacgta gttctctcgt tggcgggcat gcaggtggta
     8221 tgagtggatt agaaggtgga aaagatgaga cggacagcaa aaaaattcat tgactataac
     8281 gagtatcgag atcggccatt tggattaaaa tgggggactg cttttgcgat ggaagaacta
     8341 gtcaacggta tcaaaaaaaa tgaagaagaa gcacgcaaac agcctcttcc taaacaacaa
     8401 atgacattgc aagaaatcga tagtatactg acagaagcct ttttatctcg taaagcagta
     8461 atcattcagc tcaatttaaa agatgaattt ggaagactct tggatgaggt cgaagggcac
     8521 ttcattggtg aagcctatga agattacttt atgattgacc atcagccgat attgtgggaa
     8581 gacgtacggt atattgagtt aaaggaaaca aaaaaatggt ttgtgacatg agaggttgtg
     8641 acagaagtga tcaacttcaa aaaataagaa aggatttttc gaaaattgct tcttaaattt
     8701 ttgtggaatt ttggcttatt tttcgaagaa gcaacttctg gaacaacgta tattagatat
     8761 cagaggttgt gaaaaagtcg cttagttttg agtaacaagc caaggcgctg atttcacaac
     8821 ctctgtctat ctatccatct attcggtaag attcatgcat gacgtcttct ttatttagta
     8881 tgtagagccc tttttcttct gaccattcaa cataaatcag ctgttttaca tctgcatagc
     8941 cttctttctt caagttctcc ataagccatg cttcagtcaa ttgattggac tgaagcactt
     9001 tatgctgaat ttgcccttca tctacaagca ggatcgataa agctttgtct tcatctgatt
     9061 ttttcaatac actcatttga ccagcattct ctaggataac atgtttggca ttttcaagag
     9121 aaaagcattc ttgctgtctt aagattgctc gcaactgctc catctcgata tggttatttg
     9181 tcaactctgt catgttgatg acgccgtctt tgattagaac agatggctcg cctttgaccc
     9241 aacgattgac cttttcattt ttttggacga tacgttcgat accataaatc attactgccc
     9301 aaagcgcaat ggcaaatagg acatgcccta catggacatt gtcatcatag gttgattctt
     9361 ctaagatccc acctaaaaca agcgtataga ctaaatcaaa cggtgtgatt tctgacatgg
     9421 atttcttccc aagcaaacgt accactaata ataaaccgat caacccgaac accagtttca
     9481 ttgtaattga aaaaatcatt ttttctcccc ctaactatct gttaattaaa ttatatatgc
     9541 tttctcacga acacgcatgg aatatacaag gaagaaatgt atccttttac taaaaaaagg
     9601 actgtgacac tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactcc
     9661 ttttttttat gctctaaaat gatattttta cacaaccgtt acggttgttt tacgaaattg
     9721 gacgagactt tgccagttta atttctgttt tatccgttca ttattgtagt aatagatata
     9781 gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt gaaaaatctc
     9841 ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt tccctttccg
     9901 agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt tcatttgata
     9961 cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag tggtctggat
    10021 agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga tacgatagga
    10081 taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct tttgacggat
    10141 aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg tgattttctg
    10201 atggcagata ctggtgtaga aacgacgatg gatccgattt ttggctactg ctccgacttg
    10261 tcctcgataa gaattgtagc gccgtgattt acgtgtgtat gtcgttactt ttattcctag
    10321 cttcttgatc aggcgctgaa ctttcttttt attgatagag aaaccgcggt ttcttagttc
    10381 attggtcatc cgtaaacagc cataatcctt gtgcttctcc cgaactttaa gcatttccgc
    10441 ttcaatctct tgatctggat ttttacggtc caatcgtttt tgccagtaca taaagattgc
    10501 cttagggaac tttaaagttt ctagaagctc aactaattta aacggtcctc ggaggcttgc
    10561 gatgattcgt gcgattgctt cattcgtcgt tgttgggctt cctgttttcg cagcttcctc
    10621 aattctttta aaaaggcatt ttcaatttga agggaacgga ctcgtttctc caactcctcg
    10681 atgcgttcac gttctttaga cgttgcttta gttgtagatt ttttcttatt tttgggttca
    10741 tttttcttag acaagatagg tggacatcct ttcgtttttg agagtccatc gatcccaccc
    10801 tcacggaatt gacgcatcca actagcgatc aaattagggt tgttcatgtt aagtgtgttg
    10861 gcaacttcac gataggacag ctcacttgtt agatataact ctatcgcatc cagcttaaat
    10921 tgaacagaat attttttctt ttgtcgcttt cttactagac cctcttcacc aaattcttga
    10981 taggcattga tccagcgctt cacttgtgat ggagccttaa tggaatattt ctttgccaga
    11041 taatcacttc cgcctttacc atccaagtaa tcatgaacga tctttaattt aagttcaaaa
    11101 gtatacttag ccataaaaaa taccgacctc caaaagttag attttttggt ctaacttttg
    11161 ggggtcggta caaaacttaa aagttttaga cccttttatt ttcttaatga cttcagtcag
    11221 ttttgtgcgt aaagcgtgcg aaacaagaaa accgcgcgtt tctacgcgtg gaagaagtat
    11281 ctttagcgat aaaaactcct ttcgatatac tagtgagtgg ctaccaaccg ctcatcagat
    11341 cgaaaggagt tttttcatgt caaatgacga taaaagttta gcatatactc gttggaattg
    11401 taagtatcat attattttta caccaaaata tcaacgaaaa gtaatctatg ggcaattacg
    11461 aaaagatctt ggaaaaatac ttaggaagtt gtgcgaaatg aaagatgtgg aaatcataga
    11521 agcacatgca atgctagatc atattcacat gcttgttcga gtaccaccaa aaatatgtat
    11581 atccagtttt atggggtatc tgaaaggtcg tagtgcggta ctaatccacg agcagcacgc
    11641 aaatctcaaa tataaatacg gaaacaaatc cttttggtcg aagagatact atgtgagtac
    11701 agtcggattg aatcaaaaga caaatgaaaa atacatccgt gaacaagaag ctgatgatcg
    11761 cattcgatat attataagta aaagagagta cgtagaacca tttaaaaaat aaagagaagt
    11821 aagtgagtga agatgagcag ttggcggtct tttaaaaatc cccttttaag gggcagcgag
    11881 tgtcgacccc ttctaggggg attaaaaata cacccgtcct cacgggtgta tgggttactg
    11941 atagacttga ttttaactgg tgtcagaaac ttatgtccag aaagggtgat gaaattttgg
    12001 caatttacgg ctattcaaga attagcacaa gcaaccaaga ttacaaaaca caaattcaaa
    12061 cattagaaaa tgctggcgca gaaataatct tcctagaaaa tcactggatt atcgaacacc
    12121 tttggaagta tttttgagtt acgtaagtat tgatgatctg tctaacttaa tttgacaatt
    12181 aagagtatat ttacttcaga tggtaaggca acaaaataaa atacaatagg cagatgaact
    12241 aataaataaa atctgtatta aatacagata aattaatata aaaatcaaaa ggatcataaa
    12301 cttttaacaa gtgatgaaat gggtggaaat tgattcttat agacagtttg tctcataaca
    12361 ttgaagtatt ttgcaatttt agcattgaaa cactttttta atcttcaacg ttgttcttat
    12421 aaacaagatg attgggaatc ttttgaatta acactgtata gcttagaaca ccctttatag
    12481 aaaacattcg cttagcaatc ttaataattc cttgtgcccg atgtcgttta gattcagtag
    12541 gttcctgctc atcgatcatg gaaaaaattt gaaggatcag atccttaata aatttattta
    12601 agagaggatg atccattgct tcatccatac tcgataaact agtgataatg agctacacgt
    12661 ttttctttca agttgatcaa tagcttccac gataaaaata tctttagata aaatcaagaa
    12721 ccgtaggaac tacagggata tttggtcaat aagagccacc gctattagta aaaaataagc
    12781 tatcaagtat gctctcatcc caagaattgc ctacttcaat cgttaaaaac cgttaggttt
    12841 agctaagtgg gggtagttca ctcaatactt ttatcaataa tagagcgcaa aactttttct
    12901 cttaattcaa ctgaatcaat attattgttt tgttcataac catgaactaa tagatcacaa
    12961 atataaagtt gtgatatttt tccagctaaa gatccaccat taagaaattc ttcaatggct
    13021 gtttgtaaaa ctaagtctgc agactttcct ataggtgaat gaatatagtt agttatagca
    13081 ataatttttg cgccattttt tttagctatt ttcaatgaat cataagtatc tttcgttttc
    13141 ccagataaag aaaaaatgat tactaaatca ttaactgtta ataaagaagc gacttgggct
    13201 tgaaaatgag ggtctagtac agcttttgct tgaactccta cgcgaagaaa catattttct
    13261 aaatcaagac tagtatttcc actagatccc actccaaata tatatatgtt ttttgattga
    13321 gtgatcattt taatggcttc attcaatttt tcttcgtcta aaagcctaat agtggaatag
    13381 agagcttctg ttaaactatt agcaatttca tcatagtact ttacactagt cggatgttct
    13441 tttttttgac taaagtcttc ctttgcaatt tcaattttta aatcagaaaa accagaaaaa
    13501 ccaatttttt gacaaaagcg aataatagta gcatcgccaa cttttgtgtt tttcttaata
    13561 tcactcatcg tactataaat aatagttttc ccagaattta aaataaaatc agcgactttt
    13621 ttttcagact tcgttaaaaa tggataattt tctttaatta aatagatata actcatacaa
    13681 actcctctac aatttcaact tttctccatt atactccaaa ataataaaaa aaactcctaa
    13741 atactccatt tttatcttta cttttatgaa agcggatgct atattgtttt tggagttgtt
    13801 atccaaaaat aataaaaaat tggagtgttt aggataatgg ataaaaagag gtttttagat
    13861 cttatttcaa aaggtttgat tgtttcctgt caagcattac ctggtgaacc attttacaaa
    13921 gaggaagaaa gtttaatgcc ttattttgct ttggctgctc aaagggctgg agcagttggt
    13981 atacgtgcaa attctgttcg agatattatt gaaattagaa agtgtgtatc attaccaatg
    14041 attggaatta ttaaaagaga ttatccacca gaatctccat tcattactgc cactatgaga
    14101 gaagtagatg aattagtaga aaccggtgtt gaggtaatcg ctcttgattg cactcttaga
    14161 aatcgacatg atggactatc tataaattct tttattacta aaataaaaga aaaatatcct
    14221 catcagttat ttatggcaga tatttctaca tttgaagaag gcaaaaatgc atataaagca
    14281 ggaatagatt ttgtaggaac tactttaagt ggatatactg aggaaagtaa aaaacaagat
    14341 gggccagata tagatttgat taaaaaattg gtagaagaag ggatttgtgt aattgctgag
    14401 ggaaggattc atactccaga acaagcaaaa gagataaaaa aaataggagt tgcaggtatt
    14461 gttgtgggtg gtgctattac tcgaccacag gaaattgcat caagatttgt taaagcatta
    14521 agttaggggg agaaataaaa tgtttaaaaa attttcacaa ttaggacgcg ctttcatgtt
    14581 accaattgct atattaccag tagccggatt actattggga ctgggaggag cgttaacaaa
    14641 tgagagtgct ataaatgctt atccattttt agatcaaccg tggttgcata caattttaag
    14701 tattatgagt tatgctggaa atgcagtttt tgcaaaccta gcactaattt ttgcaattgg
    14761 tattgctgta ggacttgcaa atggagataa aggaacagct ggtttagctg gtggagtagc
    14821 ttatctagta tatactgcaa ctattagtgg gtttcttgaa cttttttcag caaaagatgc
    14881 aacactagat acaggtgtag tgggatcaat tgttgttggt ggtactgttg catttttaca
    14941 caatcgttat agaaaaattg aattaccaca attcttaggt ttttttggag gatcaagatt
    15001 tattccgatt gtttctagtt tagctgcaat cattattggt tctttctttt atttaatttg
    15061 gccaccaatt caaaatggtt tagtagttgc tggtgaacaa attgcccaaa tgggtagttt
    15121 aggaactttt ttatatggtt ttttattgcg tttaacaggt gctgttggac tacaccacac
    15181 catttaccct ttgttctggt atacatcttt aggtggtacc gaaactgtag ctggatcaac
    15241 aattgctgga gcccaaaata ttttctttgc ccaactagct gatccaaacc acactggtct
    15301 gtttacttat ggtactagat tttttgcagg aagatttgca acaatgatgt ttggattacc
    15361 agcagcatgt tatgcaatgt atcgtgcaat tcctaagaaa aatcgtaaaa agaatggtgg
    15421 gctttatttt agcggggccc ttacatcatt tttaactgga attactgaac cagttgaata
    15481 tatgttttta tttgttgctc cttggcttta tgtgattcat gcattcttag atggattatc
    15541 tttttatttt gctgatatct taaatattcg tgtagggaat tccttttcag gtggtttgat
    15601 cgacttttta ttattcggtg tacttcaagg gaatgacaaa actaattggt taaaattaat
    15661 tccttttggt attgcatggg catttatata tttctttgtt tttacattct ttatcaaaaa
    15721 atttaaggta gctattcctg gaatggaaga agaatcagaa gttcattctg aaaaagtaat
    15781 aaacaaaaat acatcattac atgaagagtc tcttatcatt attgaagctc ttggtggaaa
    15841 agaaaatatt gagtctgttg aagcttgtgc aactcgtttg cgtgttgctg ttacgaatgg
    15901 ggagattatt caaaaacaaa caataaaaga cactggtgca actgctgtct tcgaagtgaa
    15961 gggtggcatc caagctgttt ttggagggaa agctgactta ttaagtcaag aaatcaatca
    16021 aatattagga aaagataatt aggaggtttt taatatgttt gaaattttca aaaagaaaaa
    16081 acaagaaact ttttatgcac cttgttccgg aaaaatcatt tccatcacag aagtaaatga
    16141 tcctgtcttt tcacaaaaat tactaggtga tggttttgcc gtcatacctg atggttcaaa
    16201 aatttatgct ccattggctg gtcgagtaac aaatatattt cctactaaac atgctattaa
    16261 tataaaaagc aactcaggca ttgattattt gattcatatt ggaattgata cagttgaatt
    16321 acaaggagag cctttttcga tttgtgttca agaaaatgat gaggttactc ctgaaacctc
    16381 gttggttgat gtggattttg acaaaataat aaaatctggt aaagatactt ctgttatagt
    16441 tgtttttccg gagaaaaatc agttcaatga tttaataata gataatggtc gaattgctca
    16501 tgggaattca tgtggaaaaa tcacttttta aaaaagtgta tataaaaagc tataaaataa
    16561 gctaaatagg atatttgtca aatgaagttg gtgaacagaa agaactgttt tctttctgtt
    16621 cattttttta ttttattagt aatgattccc catgccaaat catgatccgt aatttaaagt
    16681 tataaaagct tcgaaatcca ttggcatttc gttggaatac cttgatatga ttattcaaat
    16741 ataaaatata gagagaagag agagagggaa gatatttata aagggcaaag ttatgtttac
    16801 tcaacttagg aaatagaaat agatcaattt atattctctt gtcaatcgaa tatgaacctt
    16861 agcactatat accaaatata gtaaaatgct gaaagatg
//
LOCUS       DACDKL010000046        16798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDKL010000046 DACDKL010000000
VERSION     DACDKL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.046"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..317
                     /locus_tag="IV329_001155"
     CDS             <1..317
                     /locus_tag="IV329_001155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940350.1"
                     /translation="LRTQISPAEFQVILFNSLYIKRGFGLGIQLIGSGFFGDDFDFET
                     NQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELGSVEYKKIANFESLYKLFNET
                     KL"
     gene            361..1533
                     /locus_tag="IV329_001156"
     CDS             361..1533
                     /locus_tag="IV329_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8940351.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1520..2224
                     /locus_tag="IV329_001157"
     CDS             1520..2224
                     /locus_tag="IV329_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8940352.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2229..3389
                     /locus_tag="IV329_001158"
     CDS             2229..3389
                     /locus_tag="IV329_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8940353.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3465..3743
                     /locus_tag="IV329_001159"
     CDS             3465..3743
                     /locus_tag="IV329_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8940354.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3724..4095
                     /locus_tag="IV329_001160"
     CDS             3724..4095
                     /locus_tag="IV329_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8940355.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4111..4515
                     /locus_tag="IV329_001161"
     CDS             4111..4515
                     /locus_tag="IV329_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940356.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4512..4922
                     /locus_tag="IV329_001162"
     CDS             4512..4922
                     /locus_tag="IV329_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940357.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4983..5570
                     /locus_tag="IV329_001163"
     CDS             4983..5570
                     /locus_tag="IV329_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8940358.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5643..6110
                     /locus_tag="IV329_001164"
     CDS             5643..6110
                     /locus_tag="IV329_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8940359.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6156..6464
                     /locus_tag="IV329_001165"
     CDS             6156..6464
                     /locus_tag="IV329_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940360.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6659..13393
                     /locus_tag="IV329_001166"
     CDS             6659..13393
                     /locus_tag="IV329_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8940361.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13397..14269
                     /locus_tag="IV329_001167"
     CDS             13397..14269
                     /locus_tag="IV329_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940362.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14266..14893
                     /locus_tag="IV329_001168"
                     /pseudo
     CDS             14266..14893
                     /locus_tag="IV329_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14854..15315
                     /locus_tag="IV329_001169"
     CDS             14854..15315
                     /locus_tag="IV329_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940363.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            15319..16143
                     /locus_tag="IV329_001170"
     CDS             15319..16143
                     /locus_tag="IV329_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940364.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16161..16706
                     /locus_tag="IV329_001171"
                     /pseudo
     CDS             16161..16706
                     /locus_tag="IV329_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         6007 a         2578 c         3572 g         4641 t
ORIGIN      
        1 cattaagaac acaaatttct ccagctgaat tccaagtgat tttattcaat tctctatata
       61 taaaacgagg ttttggctta ggaattcaac ttattggaag tggttttttt ggagatgatt
      121 ttgattttga aactaatcaa cattttgaaa caagtatcaa tgaacagtgg tttctatcac
      181 tatcaactgt tgacagtgat aactctataa agcgacaaaa attatctgaa tatattaaag
      241 aattaggatc cgtagaatat aaaaaaatag ctaattttga aagtttatat aaactcttta
      301 atgaaactaa gctctagaaa tagggctttt ttatttttct ttgaaaggtg gtgaaaacat
      361 gtgagcttat ttgatatctt taagcagtca ataagaaatg aagagccttc tgagtggata
      421 ccggattttg tatatggaga ggaagtatct gctcgggcat acctcaaaat tatggcaaaa
      481 aatactgttt tagattttgt tgcaagaact atgtcaacgt tagaagttaa gtttaaaaat
      541 aaagatggga catcagactg ggattatatt ctaaatgttc gacctaattc tgatatgtca
      601 gcagctacat tttggcaaaa atttttttat cggctcttag atgacaacga agtgttagta
      661 atatttacag atgataatca attgctgatt gctgatgatt ttagtcgaac ggagtatgcc
      721 gtgtatgatg atgtttttac aaatgtgatg gtaaaaaatt ttgtctttga acgttcatgg
      781 aacatgtctg acgtcattta tatggaatat aataacgaag aattagaaag gtttacacga
      841 ggtttatttg aagattacgc cgcgttgttc ggacggattc tagaagttgc aatgcgtaac
      901 aaccaaattc gtgggtctgt atccattgat tcaacgggaa cggcgaatga tgaaaaagga
      961 aaagatggta agactagatc tcaaagatta caagaatata ttgataggat ttggaactct
     1021 tttaaaacta actcagttgc aattgttccc aaagtgaaag gctttgagta tgaggaatac
     1081 accaataaac aaggcgtatc gaatcaatcg ctagatgaac tgaataagat gaagtcgtct
     1141 ttaattgatg atgttgccaa cgccatagga gtacctacgg cgcttattta tggtgaaaaa
     1201 gctgaactgg attcaaatat taaagctttt cgtaaattgt gtattgctcc actagttaaa
     1261 aaacttcaag acgaattggt ggcaaaaata attagtcgtc aagagtacaa aaacggggaa
     1321 cgtatagaag ttaaaaaagt tctccaaaaa gatctattag agtatgctac tcaaattgat
     1381 aaaataacat ctggtggtgc gttttatatt gacgaagttc gtgatgaagt tgattatgat
     1441 gagttgccaa ataatgaagg aaaaattcgt attcgtacga aaaactatga agaaactgtg
     1501 aagggaggtg agaacgacaa tgccgaaagc taaaaaagta ccatttcagt ttactaacga
     1561 aattcagaat ggtaagcaca ttctcacctt gagtggaaat gtacagaaaa aatattggcg
     1621 tgatgatgat gtcataaacg ctaaagatat tcgtgactct ctagatactg taaccgatga
     1681 tattgtaatc aagttgaaca gcccaggcgg tgatgtgttt gaagggattg agatttacaa
     1741 ttatttaaaa gatcacccat caaatattac tgtagaagtt acagggcttg ccgcaagcgc
     1801 agcaacgttt attgtcgctg gtgccaatga tgtgattatg aatgttggga cttctttgat
     1861 gattcatgaa gcttctacat ttgcttgggg gaataaacag gacatccaaa aaacgttaaa
     1921 cgcattggaa acgatcgatg attctatctt agctatttat tcagataaga ctggccaatc
     1981 tgttgataag ttgcgtgaat ggatgaatga agaaaaatgg tttacagcag atgaagctgt
     2041 caaatatgga tttgccaatt ctgtgaagcg tgaagaacca caaaatgaac ctcaaaatat
     2101 tgcagcgatg attcaagatg ctgttgccgt tgcaatagcg aatttaaagg aacctgttac
     2161 taataaaatc gaacaagaat caaaacaaaa atcattaatc gcacgattgc gaaaaggaga
     2221 ataaagatat gttaaaaatt acagataaaa ctgcagatgc aaaaaaggca tttaatgcta
     2281 tttcttcgaa agaagatgca acacccgaac aaattaatgc tgctctagaa gaatatgtaa
     2341 ccgccattgc agaagatgcc ggcaaacagg tgcgtgctga atatgaagaa ttgaaaaatg
     2401 tgacggacaa ccgtgtatta gaggctcgtg gaattcctac actaacagca gaagaaacaa
     2461 agttttataa tgaagccgta aaatctggag gtttcgattc tgacgtagtt tggccagaaa
     2521 cgattttgga acgtgttttt gaaaatcttc aaaacgatca tcctatttta ggcctcatta
     2581 actttacacc aactgtaggt cgtgtcaaag taattagagc acgtcgtaaa ggtgttgctg
     2641 tttttggacc attgcataaa gaccttgaag gccaactaga tgctgaattt ggtgcaactg
     2701 aatttgttca acttgcgttg accgcatttt tcttaatttc taatgatacc ttagatttag
     2761 gaccacgatg gattgaccgt tttatcaatc tttctttaag tgaagcagta cgtgatgttt
     2821 gggctaaaaa aattattaca ggttctggta aagatgagcc aattggattg cttaaagatc
     2881 tagatggtgc ggtcacaagt ggtgtatatc cagataaagc tagcgcaggg acattaactt
     2941 ttgcgaaaga gaagattgtt cttgaacttg caggggtgat gaaaaaatta tctaaataca
     3001 catacaaagt agatggtgac gatgaaggtg aagtgaaata ccgttctgtt gctggacgag
     3061 tgtatctaat tgttaatcct gttgattact gggatatcgt agctagagtt acatttgcaa
     3121 atttaaacaa tgtgtatggg actacgatgc catttatcgc tgttgatcat atcatcgaat
     3181 ctgtagacgt accagaaagt aaatgtattg catacgttga taaagagtat gaagctactc
     3241 aatcaagagc tgaaaaagtc tatgtatata aagaaacttt tgcaatgaaa cgcgcaactt
     3301 tgtatgctgt cgacatgctt ggtaatggct atccaacaaa taatgatgca gcacaagtat
     3361 atgacttgga ctttacagcg ggggagtaat gtcccctgca attggtgaga taacaccgac
     3421 gactgatggg gcagtcatta atctttcgta aatggggtta gagtatgaac actgaagaat
     3481 ttataaaaga atataaggcc cgatttcgta ttttccatac ttccgaagat gaggatatcg
     3541 ggaaacagtt agagagcggg tttgccgata taaaatctct aatcggcagt tttgatcctt
     3601 ccacttatga gaaaggaaaa gaattagttt atgaacgtac tcggtatcta cggaacgaat
     3661 cactagagta cttttacgac aattttcaaa caatgattat ggatgcatca attgatctgg
     3721 caggtgctgg atatgccaat taaatcaaat tataagaagc ctaaaattgt agctggtgat
     3781 ctaaatacgc cagttacttt ttttgagttt caacctaata agggtccaga acctggtgat
     3841 gaagaaaaaa aggaactgta tttttgtaca gcactagttt acaacccatc aatgaaagat
     3901 cgtgacattc ttggcacaaa agaaggcgtt acgataaaaa tacgggatcc ccatcaatct
     3961 tatattccta ctaacaaaca taaagtaagg atattagatt atcgatatgg agataaagtt
     4021 tgggaaataa tcgatgtctc accggatttg gaagaaaatt caattgtaaa gatagtatta
     4081 gaattaatta cttaaatttg gtgataaatg atgagtaacg taaagtttga aggcgttcaa
     4141 gaaacaatcc aagctttaga aaaaaagtat ggagaaaaga aaactaagac attaacaaaa
     4201 aaagcaatca atgttggcgc tgaaaaagta gaaaaacagt tgcaaacgga catgaccgtt
     4261 tttaaagaca aaggttatac gatcgctgag gttgttcgca agaacgctac gtatagagat
     4321 tacaaggcag aagctgaaat cggttggaat ggtccacatc aacgatatag aatcattcat
     4381 ttgaatgaat ggggatatac acgaaagggc aaacaaatta gaccgagagg atttggtgta
     4441 ataacaaaat ctcttaagaa ttccgagctg ttgtactttg atgctgtagg agaggaggtt
     4501 aaaaagagtt tatgaaagat atgcttaata tcatttacga ggctctttgt ttgaatgagt
     4561 atattcattc aatgacctac aacgagaata ccgagcaata tcgtattaaa ttttatgagc
     4621 aacctgaaac cgccgataaa acaggtccaa tgatcacaat tcgtcctgta gatacaccta
     4681 acgaagcgta ccacggaagt aacaaagagc tttctgtgga gcatatgata cagattgacg
     4741 ttgaatcgaa atatagagca acatgcaagc agattcaata tgagataaaa aaagagatga
     4801 aaaaactagg ctttggccaa atttccggtc aagggttaga tgaatacttt actgaaacaa
     4861 accgctttgt agacgcgcgt cgttacgaag gaaatacaaa aatttacgat accgaatatt
     4921 agaaattaag acacgaaaat aacgtgtctt tttttattac ccaaaattag gaggaaaatt
     4981 ttatgacttt agtaggattc aaaaaaatga caatcggggt ctttgacagc acagggaaga
     5041 ttccagcagc aaatttatat gtaattgaag gtgaacaaaa caaaggggcg actgtatctg
     5101 ctgaaatcag tgggctatca aaagaaccat caaaagttta cggatcgaac atcgcttact
     5161 atgtctctca aaaaggaaca ggcgatgttt cagctacttt tggattgtta gatttaccaa
     5221 cagaagtaaa tgacaaaatt cttggttaca aaaccgatac aaacaaaatc agcttcttgg
     5281 gagaagatac tgagccacca tattgtgcaa ttttgatgga atcggaggat ttgaacggcg
     5341 atacagcaat gcttgcgatg ttcaaaggaa aattcagtcg tgaatctatt aatcttaaca
     5401 ctacgacaaa tgaagctttc gaaccagaag cagaagaata tgtattttca gcgattgcca
     5461 atgatgttga aggtgatgct aaaggccaaa cagtcgcaaa atatgttgga gacgaagaag
     5521 cttctattac tggtttgaaa gagatggtat ttcctgcagc gggggagtaa ctagccccgt
     5581 tgttggagca atcactccca ctattaatgg ggcaacagtt gaattaagtt aggaggataa
     5641 ctatgccaga tacatttaaa atctataaaa aaaatggtac tgaatttaca aaagtcgcag
     5701 agggtgaaag tccactttct attactggaa ttgcagcaaa tactcaagtt gctaaaggag
     5761 attatcaagc tacgcgtgtg gtcggaggtc aagaatcagt aaaagtagat attcctgcat
     5821 tcaccactct tccaatttca gttactggtg tgacgttgga taaaacgaca gctgaagttg
     5881 aacaaggcgg aaccttaaaa ttaactccta ctgtaacacc agcaaatgca acagacaaaa
     5941 caggaagctg gagcagttcg aatacagctg tagctacggt tactggaggc aacgttacag
     6001 taaaaacaga cgctgaagtt ggcggaacta cagaagtttc atttactact acggatggcg
     6061 gtaaagtagc aaaatgtact attacagtta ttgaaaaagc agcgggctag tcttcggact
     6121 agctttttta tttggaaaat aatggaggaa tctaaatggc aaaagtaaga attgaattga
     6181 aagaagaatc aggaaagaaa gtctatgaaa acatagacac aacagggaaa gattatcgaa
     6241 aagctttaga aacaatcaaa aagctaaatg aagacggagt aatgatttgg aatcgtttag
     6301 acatttattt ggattttgct gtaggtatct tcagggatag caaattgaca gctgaacaaa
     6361 ttcttgatgg cttaccatct gagaaggtaa tgagcacatt ggacgacatc ctaggagaag
     6421 taatggggat tgaaagtaat ccagacccag acgcaaaaaa gtaacacccg aagaagcaga
     6481 agaaatgtac ttaaacctat gcagagagtt agtgaaagcg ggatggtcac tatctgacat
     6541 agaagataat tcctttgaaa tgatgatgaa aattgcgtgc acttcgccta agaaagagaa
     6601 acaaaaagaa gttgatctta aagacttctt gaaatccatg tagagaggag gtaaacatat
     6661 ggcaaatgga aaacctttag gcaatatgat tatcaaacta gacttagaca gttcagcttt
     6721 ttcaaaaggt ctagccggag ctaaaaacgc tgttaaccat caaatgaaag caatgaaatc
     6781 acagatgcaa gtaatgaact cttcaggaaa ttctctcggt gctttacaag caaaatacaa
     6841 tggtttaggc ggagttctac aagcaaatga aaagcaagta gaattgctca ctaaggctta
     6901 taaggatagt tttgactcta acggaaacgc tactgcttcg actgctaaat acgctaatca
     6961 attgaatcaa gcaacagcta gatcagcaag ttacgaatct cagatgaaaa caactgttgg
     7021 acagattgct cggatgaaag tggaaacgga agggtttacg gggaaactaa aggctaattc
     7081 tgatgtattg attaattctg ggaaaaaaat tgaagccttt ggtaaaggag tttctgggat
     7141 aggatctgcc ctaactgttg gagtaacagc tccaatagtg gcaggcgcaa ctgctgttac
     7201 tagtgcagct atttcatggg aaagtgcttt tgcaggtgta aaaaagacaa atgatgaagt
     7261 tgtagactcg aatggaaacg tagtttattc ctatagcgac ttagagaaag gtctgcgtaa
     7321 tcttgccacc caattacctg gtagccatca agaaatagca aatgtagccg aagcagccgg
     7381 gcaattaggt ataaagactg aaaacgtagt tagcttcact aaaacgatga tagacatggg
     7441 tgaatcgact aatatgtcgg ctgaaactgc ggcaacttct ttagcacgat ttgctaatat
     7501 tacccaaatg tctcaaaaag actttgataa attaggatca gtcattgttg atctaggtaa
     7561 caattttgct acaaccgaat cagaaatcac tgaaatggga ttgcggttag cgggagctgg
     7621 taaacagatt ggaatgagcc aaggcgaaat tctaggattt gctactgctc tatcttctgt
     7681 tggtgtcgaa gctgaagcgg gtgggtctgc cttttcgaag gtcatgatcc agatgcaact
     7741 agctgtagaa aaaggatttg gcgcttttga tcagctgaaa cagatggcag aagaacaagg
     7801 tgttccgtgg gttaatttag taaatgctgt gagagacggt ggaaaatcgt taaaagcagt
     7861 atcagagcaa atgggattca cttcatctga tttaaagaaa atgtataaag aagcggacaa
     7921 ttcgaaaagt tcgcttgaaa attttgcgaa tgtggcaggt gttacaagtg aacaattcca
     7981 gaagttgttc aagagtgatc cttcagaagc aatcatcaag ttcattcaag ggctgaaaga
     8041 ttcagaaaaa catggtactt cagcaatcaa agttctggat gacatggata taaaagaagt
     8101 tcgactacgc gacagtttat tgagagctgc aaatgctagt gacatattca aaggagcaat
     8161 tgaaaaagga aacggcgcgt ggaaacaaaa tacagcttta acaaatgaag ctaataaaag
     8221 atacgaaaca actgaatcaa agctaaagat attgaaaaac gaagtgattg atacagcaat
     8281 cgatcttgga ggtccatttg tagacgcgtt gcgtgatggt ctaaatgtat cgaaaccatt
     8341 gataaaaaac ttagggaata tggcgaaagc tttctcagct atgagtgatg atcaacaaag
     8401 aaacattatc aaatgggttg gattagcagc agcagctggt ccagcgctga agcttttagg
     8461 aactggtact tcagtaatag gtaaagttac ttcaactact ggaagattga cgaaaagcct
     8521 tgttgattta gcagcgaaag cagcagaaaa gaaggctatg gatgcctttg catcgtcggt
     8581 aacaacagct ggtgctgcag ctgcaaagac agcaggagct ggaggtattg gcgggttagt
     8641 ccctatgatg ggaaaaacag ctagtgctgc aagtactgct gcgggcgcca gcggaatagg
     8701 cgcaatgaca acgtcactag gtttgttagg tcctgctcta cttggtattg ttggcgttgg
     8761 tggcgcactg gcagttggct atggagcatg gaaattattt ggtgaagaag cttggaattc
     8821 ctcgcaaaga gtaaagcaat ggggaacaga tgtcggtgag gagatagata gtactcttga
     8881 cggagtacag aagaatattg aaggtgcaaa tggtcaattc ggtcttctca aagagggttt
     8941 tactgttgat gatgcaaata atatggcggc taatttcgca accgctggaa catctttgga
     9001 aacatcttta acgggtagaa taaaggcatt agacgatgca ctaaaagggc tacctcaatc
     9061 agttcaggat gccatgaaag aagtagctga atcagaagaa gaaaccttag ggcaatctct
     9121 ttctaaaata aaagacaaca atgaagaaat aaaaaaaatc agagaaagag ccgcaaatca
     9181 gaacagagac ttgactgttt cagaactaca acagatacag gatctaatga aaggaacttc
     9241 tcaagcgtat gtggatacgc tcgatgtttc tgctaaagac agaaaaaaga tactatcagc
     9301 catgaatggt gatgttgcac aagctacaga agaagaagcg aagacgtggt tacaatcttt
     9361 agcgaaacaa aaagctgcaa ctaccgaaaa ttatagacaa atgcttgaag aaaaaaaggc
     9421 ggctttaaag gaaaagggag taagcaaaga attcattgat gcattagaag atgaaatgaa
     9481 tgattacatc aaaactacaa atcaaggatt cgatagccaa atagctgcta taacaaataa
     9541 atatccacaa ttgattaacg aagtgagttt atcaaatggt caattattta gcaaaacggc
     9601 agctgctatg gacaatactg gtcagtatac aaaagttttt ctcgaaagta atgaaaagat
     9661 tttagcccaa gcgaagaaaa catcggatga aatagcagat aatgccgaaa aaaatgcaga
     9721 acaacttggt atggtagctg atgaggctac gcatgctgga tcaacttgga atggcttagt
     9781 tttagacgaa aaaacaggaa aagtaaaaac taatgtacaa gaagtagtga atgaagcaac
     9841 gaaggatttc actaaatgga atgaattgaa acctgttgtt catgatgcga aactaacaac
     9901 gaatgcaaaa gatgtcattt ccatagcggc agtacaaaat ggctattggg atgatatgga
     9961 atggaaagaa aaagaagcat tattagaaga tgagtgttct aaaaatgtat acaaggcttt
    10021 agaaaatgct ggtaaatgga atgagttaga tattccttct aaagaagcaa tcctaacttc
    10081 aaatactcct gaagttatgg gtgaaactct catgaatttg ggattatggg atacttacaa
    10141 acttcaagtg aaagatttag atttgaatgc gtataaagta tacgatgcta tcgatggatc
    10201 agaagaaaaa ttaaatagtt gggctcaatt agataacccc acaaaagaat tgttattaga
    10261 taactcagat ttttctacaa aactttttaa ttccgaagaa atgtttaaac gttggaatca
    10321 gttatcatct aatgcaaagt ttctttcggc taataatact aaatttacag aggttctttt
    10381 atcatctgac agaatgtgga atcaatgggt aaaccttcct gataatgaga aaagattact
    10441 tgcagataac gaagatttag ctaccactgt tttatcttca gaagaaatat acggaagatg
    10501 gactcaatta ccagataatc aaaaatatat gttagctgac aatactgact tagcaaacaa
    10561 aatattcgct tcagaagaat catggaatgc atggaaagca attccagaca cagttaaaaa
    10621 cttgcttggt aataatattg atttactggc taaagtgaaa gacgggactg taagtgtaga
    10681 agaatataac aaagttatgc cacttcttaa aactctatat ggagataacg ttaatttaaa
    10741 agaggcaatc agtggggcta aaagctctat agagaattac aacaaaaata tttttcctag
    10801 tgataaaact gcagtaggac acgatgaggc ttcacaagct gctaaagatg catttgaagc
    10861 gttcaacatc ttccaaacaa aaataccaga taaaattact aaaactgtat cagctgactt
    10921 tataggtcct atgccgaaaa atgcgaaagg aacaaacttc catcctggcg gagcagctat
    10981 ggtaaacgat caaaaagggc ctacttatga agagcttata accttgccaa gcggagaaac
    11041 ttttatacct aaaggacgta atgtcgtttt agacttacca agaggttcga aagtactgaa
    11101 cgccactaaa actaaacgtc tagtgcctaa atatgctgat ggtataggaa acataacgac
    11161 ggtttcatct acgctaaact tagatgcttt aattttagct attaacgagc taacgacggt
    11221 attgagaact caactgatgg caggaacgac tgatgctaaa gatactaaac tatcagatga
    11281 aacaaaagtt gccgatccga ttattccgga cagtctttct gaaaaatcag atcagtatct
    11341 aggaattgga gctcaatggt taactaatct aatgaatggt tggaactcag cggttcctca
    11401 atatatgaat agtgaaatgg tttttattac aaactatctc aatgcgctaa aacttcaaaa
    11461 caatcctaat tatacgcaag gagcaacatg gaataagaac ttactgaatg gatggaatag
    11521 tttaactggg acctttattg ctacaatcaa ttcattttgt aatcaagcaa tggtcacgct
    11581 tagaaactac aacactccta tgtacaacaa tggccgaacg tggcagcaaa ataatcttaa
    11641 tggttggaat tcattgtatg gatcattcat agcccgtgta aatcagcttg gtaacgattc
    11701 gattaacaac cttcgttcta aaagtggtgg tttttacaat gcagggacat tcttgttgca
    11761 atctttaata aacggaatga attcgttagg taattctctt tctactacaa tgaacaatgt
    11821 cgctaataca atggttggtg gtataggcaa aggagttaat ggcgtcatat cgggagttaa
    11881 ctatgtgctt aaggaagtcg aatcaagtaa gaatatcgga aactggccaa ttccacaata
    11941 cgctaaggga actgatggac atcctggtgg acttgctatg attaatgatc aaaaaggcct
    12001 agttcacgaa gaatatgttc agatgccaga tggacgtagt tttattgcca aaggtagaga
    12061 tcttcttgtt aatttgccta aaggtgctca ggtcttaaat gcttctttaa caaagaaatt
    12121 aaaggaacgt ttaaatattc cacactacga aaaaggtatt ggcaatttag atattgttga
    12181 tttgttagat gatgaaaaaa gaatgttaga attcttaact agcaaagttg atttttcagg
    12241 cattaatgaa ccatggcttg atatgactaa atcaggaacg tcattaatgt ccaaagctgc
    12301 aaatacaatg cttcaatcaa aattaagtga attcttcact catggcaatt ttgatggtgc
    12361 agtaaatgcc aatggtgttt atcaatattt agttgatgtg gcacagaaag taatgggtaa
    12421 gttccccgga cttacagtaa cttctggata tcgagcagga gatgcctact atcacggaaa
    12481 acggcaagct atcgacttgg cttatccagg tatttctgga gatccgagat atacagcagc
    12541 agcaaattac gcttttgaaa aattcccttc aaaaattgcg tatgtcatta cgaatggacg
    12601 tgtacgtgac cgcatgggat tatctggaac aaggtcaagc ggacaatgga cgaattggcc
    12661 agatggcgat cacttcgatc atattcattt aaacggttcg atggggtctg gcgatatatt
    12721 caatggagga gttggaggaa gtggtgttgc acgctggcgt tcttatgtgt ctaaagcatt
    12781 gaaaatgaat ggattaccag ctacagcagc gtatataaat gcctggatgt cccaaatcca
    12841 aacggaatct ggtggtaatg agaaagcaat cggcggcaat gatgggctcg ccgaaggcaa
    12901 tgcaacagga ttacttcaaa cgaaacccgg aacttttgca gctaacgctt ttcctggtca
    12961 cggaaatatt atgaacggat ttgataacat gttagcagca atcaattatg ctaagaaacg
    13021 ttacggagtt gcaggtatgt tgcaagttat cgggaaagga catggatatg ccaatggcgg
    13081 cttgattacc aaagacggac tatatcgagc aggagaagga aataaaccag aaatggttat
    13141 tccattgaca agaaaaacaa gagctatcga acttatgggt caagcattag ccttcctttc
    13201 tggaagcaat aaaaaagaaa cgaaaagtga gattaattct gtagataata gacatatcct
    13261 acaagcgata gaaaaacagt ctcgtatgac aaatgatctg ctatcattat tactctcgtt
    13321 ttttaaaagt aacaatcgat cagataaaga actagcttta gatattcaga aaattttaat
    13381 caggaggaca tagtatttga ggacagtaat attaagaaat aaattgaatg aagaaattga
    13441 tttgtccaca gaagattatt ttgctactga aatgtcgaat ctaggttttg aagttacaaa
    13501 agagcatatt ggacaatggg ggagtttcag agaaatagga gagagtattg aattgtctga
    13561 atttcaatca tcagttatga tttctgttca cggatttaga gaacaacaat tatatggttc
    13621 attagttgaa tttctggcta atggaccttt tgaactagaa ttttcttttg attcagaaac
    13681 aatgatcagg aaatgtagtt tgaaatcatt atctaaaaca gaaattgatt cccaaagctc
    13741 attgctaaca gatactttga atctatattt tacttcaagc tggtattcta ttaagcgtga
    13801 aaaattaata cagaattcaa cttctctcaa tacacgtgga aaagtttact catttgttcg
    13861 accatatgtc tacaccaaaa atgtatggga aaagaaaggt gtatttaagt tcagtaataa
    13921 ttctgtatat ttgacaaata gtacagagcg aatgtctccg ttaaaaataa ggattattgg
    13981 agagtgctca aatccatatt gggaagttat ccaaaactct aaagtagtag cttcagatgg
    14041 ctattttatt aatatgaaag acacacaaat attagaagtg tctagtttgc tagaagacca
    14101 aacggcaatt ttaaaagatg taggtggagt tgaatcttct gtttatcaac aacaagattt
    14161 tactaaatca aacttcgtcc aagcgcctct aggagaattc agcgttgttt ttcatgttgg
    14221 acatgcaaat gtagagattg aactttatga ggagcgtgat ctattttgat tttagccatt
    14281 accctctttc accgagatat gaatttatat aacgaacgtt taatttcgac tgagttcgaa
    14341 tttggaacgg atgaaattgg agaagaagta agtaatttta ttacagataa atatgtccat
    14401 gtaaaatcag gagattttct actagcaaaa tttattccta gcgggaagat tgcttatttt
    14461 ggagtgatta gctcgcaaga agacaaaaaa ataaattgca aaggattact cagtttggcc
    14521 gatagcgaga taccgactgt aaaaatatca ggtgctaatt atgaagagca tattcgacga
    14581 ctgattgaat actacttatt gaacgatcca acaaaacaac taaaaaatat tttggaggta
    14641 aaaacagaaa gcgttacttc tcattcttat caagctgttg acacaaataa gagacgattg
    14701 aatgcatata taataaatgg gttcaaaaaa tataatgtta aatggtatgt caaagagatt
    14761 aaaggtaaaa aaatatatac tggtattcga gcagttgatg attcaattta tattaaagat
    14821 aattcatctg agtttaatga ctgggatgtt tttgtgcaat ctcctggacc agggaacgag
    14881 aacaaattat tgatcgtaga taaagcaatg aaagatatag aaaatccctt ggtattgtct
    14941 acgtggtatt tagatgacga aaataatctt acgcaagatt caacgaataa gaacattgca
    15001 ttgcctaccg ttaatttagt aaatatatat gatcaaactc aagaagatag accttcttat
    15061 gaagaggtag ctaaatcgga gcttaaaggc aacgcctatt cacatgaaat taaagttaat
    15121 gttgtgctaa atgctagaaa tatcaatgta gaaaatatcg aaacaggaat gttagcgact
    15181 attgtttaca aagagcagac ttacaaatct gttctaactg cctggcgaat atccagcagt
    15241 aaaaagaata tagaattgac atttggtaat gtccgaagtc gttttatgga ttattttgaa
    15301 gataatgggg gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa
    15361 gtaagcgcag aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt
    15421 attgatggtt acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct
    15481 gcaggagcag cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca
    15541 gttccagcaa actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata
    15601 cctggatcta ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta
    15661 gaatttataa caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta
    15721 ccactatgct cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt
    15781 tacgagctga ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat
    15841 actcaaactg tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta
    15901 tggctcccat acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat
    15961 aaggagcgta taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat
    16021 cacaaaaagt ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt
    16081 gcagcaagca atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt
    16141 tagttaggag gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac
    16201 agccttatct acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca
    16261 atgtacaact tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt
    16321 tgaaatttgt gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg
    16381 attcgactaa tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc
    16441 aattcaaaca agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga
    16501 acttcatcat agacgtgtta gaacgagtag atattaattc ggagcaagcg aaatactata
    16561 tttcttcatt ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg
    16621 ataaacaggt ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa
    16681 atgaatcaga taagcaagta aatgatcgta ttgataaggc aaataatcgt attgatcaaa
    16741 ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac agcattga
//
LOCUS       DACDKL010000047        16582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDKL010000047 DACDKL010000000
VERSION     DACDKL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.047"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..109
                     /locus_tag="IV329_001172"
     CDS             <1..109
                     /locus_tag="IV329_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8940365.1"
                     /translation="KLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            182..298
                     /locus_tag="IV329_001173"
     CDS             182..298
                     /locus_tag="IV329_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8940366.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(353..2035)
                     /gene="recN"
                     /locus_tag="IV329_001174"
     CDS             complement(353..2035)
                     /gene="recN"
                     /locus_tag="IV329_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8940367.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2053..2502)
                     /locus_tag="IV329_001175"
     CDS             complement(2053..2502)
                     /locus_tag="IV329_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8940368.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2609..3427)
                     /locus_tag="IV329_001176"
     CDS             complement(2609..3427)
                     /locus_tag="IV329_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8940369.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3436..4326)
                     /locus_tag="IV329_001177"
     CDS             complement(3436..4326)
                     /locus_tag="IV329_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8940370.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4328..4552)
                     /locus_tag="IV329_001178"
     CDS             complement(4328..4552)
                     /locus_tag="IV329_001178"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8940371.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4557..5894)
                     /locus_tag="IV329_001179"
     CDS             complement(4557..5894)
                     /locus_tag="IV329_001179"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8940372.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5895..6749)
                     /locus_tag="IV329_001180"
     CDS             complement(5895..6749)
                     /locus_tag="IV329_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8940373.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6846..7295)
                     /gene="nusB"
                     /locus_tag="IV329_001181"
     CDS             complement(6846..7295)
                     /gene="nusB"
                     /locus_tag="IV329_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8940374.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7288..7713)
                     /locus_tag="IV329_001182"
     CDS             complement(7288..7713)
                     /locus_tag="IV329_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8940375.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7959..9023)
                     /locus_tag="IV329_001183"
     CDS             complement(7959..9023)
                     /locus_tag="IV329_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8940376.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9195..9440)
                     /locus_tag="IV329_001184"
     CDS             complement(9195..9440)
                     /locus_tag="IV329_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8940377.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9579..9953)
                     /locus_tag="IV329_001185"
     CDS             complement(9579..9953)
                     /locus_tag="IV329_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8940378.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10036..10950)
                     /locus_tag="IV329_001186"
     CDS             complement(10036..10950)
                     /locus_tag="IV329_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8940379.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(10971..11774)
                     /locus_tag="IV329_001187"
     CDS             complement(10971..11774)
                     /locus_tag="IV329_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8940380.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11807..12796)
                     /gene="manX"
                     /locus_tag="IV329_001188"
     CDS             complement(11807..12796)
                     /gene="manX"
                     /locus_tag="IV329_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8940381.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12899..13390)
                     /locus_tag="IV329_001189"
     CDS             complement(12899..13390)
                     /locus_tag="IV329_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8940382.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13581..16415)
                     /locus_tag="IV329_001190"
     CDS             complement(13581..16415)
                     /locus_tag="IV329_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8940383.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         4617 a         3709 c         2765 g         5491 t
ORIGIN      
        1 taagttacct attgctgaaa gggatgatgc ctttatatgg ctagttctat taacattatt
       61 tatttgtatc gtaactacct attatttaaa aaaagggaag tttttataaa aaagccctct
      121 tgtggaaatg gaaaaaagat tgtacacttg aatcaactaa ttaatgacga ggttgaaaac
      181 aatgcgtgaa aaaaaacaaa caagtacttt tgctaaagta acaaaatttg tcatttggac
      241 aatgctgatt ttaacgattg gatctgttgt attgactgct gtaatgagtg tcatgtaaaa
      301 ataaaatata gctatcagct gctgaaaagg acctgaacca ctgcgaggtg gctcaggtcc
      361 tttttgcgag tttcaatagt tcttttgcat gttcgagtgt gagttttgta atctcacttc
      421 cagccagcat acgtgcaatt tcttcttctc tttcttttgt tttcaactct gaaacagaag
      481 tttgcgtacg tccatcaatc actgctttca caatataata ttgatagtcg gcgactgcag
      541 caacttgagg cagatgagtg atgcacaaaa cttgagagta tttcgatatt tgagaaattt
      601 tatctgcgat ggcctgtgca actcttccgc tcacacctgt atccacttca tcaaaaataa
      661 tacttgtcac accttgctca gaagaaaaaa tagttttcaa agcaagcaac atccgagaca
      721 gttcgccacc agaagcgact ttcaccaaag gcttcagtgg ctctcctgga ttggtcgtaa
      781 tgtaaaattc gatttggtca aatccatcat gattcaattg tctattttct tcttttaaga
      841 aacgtacctc aaactcagtg ttttccatat acagactttt caattcatgc agaatggatt
      901 tttccaattc ttttgcgatt tttttacgcg cttcatgaag atctgcggct tgttgctgaa
      961 gtagaagctc tttttgctct agttcctctt tcattttgtc caattgacct tctgaaaaat
     1021 cagaagaagc tagctcttcg gtgatttcgt catagtaagc caatattgac tcaatggatt
     1081 cgccgtattt acgtttcaac tgtcggatta attctaaacg ttgcgtaact tcttctaaac
     1141 gactctcatc taactccaat aaatcaatct gacgactcat atcgccgatt gcatcttgca
     1201 gcaaatagta agcactttgg atattttcat aaattgcttc atactccaca tctagatggg
     1261 cgatcccttg cagttctgaa acggccaagc caacaccatc tacactgttt tgttcattat
     1321 tcgtcagtgc tccgtaacct gcagctaatc catcagcaat tttttgataa ttgctaagtt
     1381 tttctctttc ttcttttaat ttctcttctt ctccgacttg caaatcagca gcagctattt
     1441 cttcttgctg gaaatggagc atatctattc tctgaacata ggatttttca ttttgctgca
     1501 cttttcgtac ccttttttcc agctcacgat aagagatata ttcttgctga taactctttt
     1561 tcttctgttg gaaagctaca tctccaaaac gatccaataa agcaagatgt gattcaggct
     1621 ggagcagttc ctgatgttcg ttttgtcctt ggatatctac taaataactg ccgattcttc
     1681 tcagattagc taatgtgata atatgaccat tcactcgaca aacatttttt ccagttaagg
     1741 acatatcccg tcgaacaatc agattgtcct catctgtttc gatccctaat tcaaccatca
     1801 gttcagagaa tccttcttgt tttggtagtt caaacaaccc ttcaagcaca catttttctg
     1861 ctccttgacg tatataatcg cttgaacctc ttcctcctgc taacagacct aaagcatcga
     1921 taataatgga tttacccgca ccagtctctc cggtcagcgc ggtcattcct tcgtgaaaag
     1981 aaagccgcaa ttccggaata atcgcaaagt tagtaatact gatttcttgt agcatcacat
     2041 tcgctcctca acttaaaggt aatgcaggaa gtctttcttc aataaaactg cctggtcttc
     2101 agttcgtgta atcatcaaaa cattatcgtc atcattgata atcgaaaata attctttctt
     2161 gtaacgttta tcaatcaaat ttgctgctgc cgaagcattt cccggcaatg ttttcaaaat
     2221 aacaaatttt tccatttggt caactgccac gaaagcgtct tttaatagtt tttccaattt
     2281 agtatttaca tcttcgtttg tttcagctgg tatgctataa cggtagccgc catctgaagc
     2341 cggtaccttg atcagtttca gttcctttat atctctggaa atcgtagctt gagtaacaga
     2401 tattcctttg cttttcagga tttcaacaaa ttcttcctgc tttcggatat cttgctcact
     2461 caacaatcga gtaatcaaac gatgccgatc tttttttctc attacgtctt cgctccttag
     2521 catattcttc ttaatcaatg atagcttatt tccttggtat aataaagaag aatccgcata
     2581 cttctcactt gattttcaca gttacaattt atttatggtc aaactgttcg tgtgcatttg
     2641 aaaccacttc tgccatatta atagctgaat tgattgttcc gctttcgggt actttcctca
     2701 agtgagccaa gaactcgata tttccttctc cccccgtgat cggagaataa cttaattcca
     2761 gcacatcata tccaccttgt gccgcaaatg acaaaatgtc ttctaaaact ttttgatggg
     2821 tctgagggtc acgaacaatt ccgtttttcc ctacagcttc tctgcctgct tcgaattggg
     2881 gtttgattaa agcaatcact tctccctctt cttttaaaat agcatgtaat ggaggcagca
     2941 tcagttttag tgaaataaaa gaaacatcga taacagcaac ttctggaatt cctaaggtaa
     3001 agtcttcggg ttttgcataa cgaaaattca ctctttccat tacgacgact ctaggatctt
     3061 gtcttatttt ccaagctagc tgattgtagc caacgtctaa agcataactc atttttgcgc
     3121 cattttgcaa agctgcatca gtaaatccgc cagtggatgc accaatatct aacaatgttt
     3181 ttccttcaac agatagatca aatgctttga gtgctttttc taattttaat ccccctcgag
     3241 aaacataagg aagtttttta cctttgactc ttaattcact actcactgga attttttctc
     3301 ccggtttatc aaaccgctcg tttttttcgt tgtaaatcaa accagccatc actgaacgtt
     3361 tggcttgttc tcttgtttca aataatcctt gtttgaccgc taatacatct acgcgttctt
     3421 tttccattct ttccctcatt tctccacatc aaattgcttg atcatctggc gcatcagccc
     3481 gccatcaaaa gaaaaaacgt cctcttctaa tttgtctatg atcttgttgg cggataacag
     3541 ctcaatctcc aacgcctcac gtgtcttttc taggcctaat aaacgcgggt aagtattttt
     3601 ttccatacgt tcatctttac cagctgtttt ccctaatgtt ttcgttgtac caatcacatc
     3661 gagtagatca tctcttatct gaaaagctaa acctaaatgt tcggctaatc gttgaagtag
     3721 caataagatt tcttcaggtt gcttcgctaa tatgccacca gccagcaagg cataacgaat
     3781 caaatgccct gtctttcttt catgaataaa tgctaattct tctaaagaaa gctttttcga
     3841 ttcgccctcg atatctgcag cttgcccagc aaccatacct tgtgatcccg cacaaacagc
     3901 taattgctga agcagcagaa gcttaggaga attacctaag tgtgccatac tgattagctg
     3961 gaatgctcct gtaagcaaac catctccggc caatatagct aatgcttcgc cgaatacttt
     4021 atgattggta ggttttcctc tacgaagatc atcattgtcc atcgcaggca aatcatcgtg
     4081 gatcaaggaa tacgtatgca ccatttctaa agcagctgct acttgataga cgggaacatc
     4141 aataggctca tcaaaggaag caacggccgt taaaagtaat aagggacgta ttcttttccc
     4201 gcctgctttc actgaataac tcatcgcatc atagagtcct ctatccgttg tatgctcatc
     4261 taaaaaagac aagatttctt tttctacagc aggtagatga atagctgaaa agtcagttaa
     4321 tctcatctca gctttcctcg tttccgtcaa aatcaacttc ttcatttgat tctgtcatca
     4381 ttttcgtcaa ggtctcttct gctttagcta atgtatcttg gcaatgtttg cttaattcca
     4441 ttcctcgttt aaacgaatct agagctgatt ctaatggaac gtctccttgt tctaactgca
     4501 tgacaatttt ttctaattct tgcagtgact cttcaaaagt tggattttcc atatttttat
     4561 tctcctttgt ctatctcttg tacttgtcct ttaacgattc catcttgata atggatcgtc
     4621 agcaaatccc cttgatgaat ctcggtgaca ctttttacta cttgatcatt taacgtcgta
     4681 tagctgtagc ctcttcccat gattttcaat ggactcaaca aatcaagaga ttgaacagct
     4741 tgagcaaaac gctgctgctg ttgtttcata tattgttgga ttttctcttc taagcgtttt
     4801 tctagataag cagtttcttg gcgagctgct ttaactcgat aaagcggagc cacttgttcc
     4861 aattggtggc ttaatgcgat cgtttgtttt tctttcgcat gataaatttg ctgtgtagct
     4921 tgatataatc gctgtttcaa ctggtctaat ttgatggtct gaccttcata caatctttca
     4981 ggctgccgaa atacatagga acgtcgagca cgctcgaacc gttcttgttt gcgctggatc
     5041 tggcgtacaa acgcctgctg caatctagct tgccgttctt gaatgcgcat caactcttct
     5101 gacaaaacag gaacagctaa ttctgcagct gctgttggag tggcagcccg aacatctgct
     5161 actaaatcag cgatcgtggt atctgtttca tgtcctacag atgaaatcac cggcgtatta
     5221 gcttgataaa tcgcccgagc aacccgctct tcattaaacg gccataaatc ctcaatcgaa
     5281 ccacccccac gaccgatgat cattgtatcg aaattgccca tctcttctac tctctggata
     5341 ttcctgacaa cattgtccgc tgcttggttt ccttgtacaa ctgttgggaa caaaaccaat
     5401 tggacaattg gatatcttct tttcaccgtc gtgatgatat cgcggataac cgctccgctt
     5461 ggactggtaa ggaccgcgat tcgctttggg aaacgtggca gcattttttt aggcccttga
     5521 aaaaggcctt ccttcatcag cttttctttt cgttcttcca attcttgata taaagcacct
     5581 attccatcag gctccatgtg ctctacatag atttgataag aacctcccgc ttcatataat
     5641 gaaatacgcc caacgacaag gactttcatt ccttcttttg gctggaatcg taatttttgg
     5701 aatgctcctt tgaacataat agcagatatt tttgcatggt catcttttaa gctgaaatat
     5761 tgatgggcat tggcacgtaa acggaaattc gatatttcgc ctgttaaata tacgcgttct
     5821 aaataaggat cggcatcaaa ttttcttttc aaatacttcg ttaatgtagt gacagttaaa
     5881 tattcttgtg ccatctattc acctgctcca aaagcttcgg cagattttac ggtttgatac
     5941 atcagcatcg tgatagtcat tggccctaca ccctttggca caggagtgat ccagcctgca
     6001 ataggctcca cttcatcaaa tttcacatca ccgatcagct taccgttagc atcacgattc
     6061 atccctacat ctatcactac agcacctggt tttacgaatt cttttgtgac aaaatgtcca
     6121 cgtcctattg ctacgactaa aatatctgct tcttgtgcca gttcagccag attttcggtt
     6181 ttagaatgag caacagtaac agttgcatct gccatcaaga gtagctgagc catcggttta
     6241 ccgacgatat tgcttcgtcc gataactact gctcgtttac ccgccggatc gatagaataa
     6301 gcttcaaaca ttttcatgat gccgtatgga gtacaaggaa tcttgtccgg tttacccgca
     6361 aggagtctgc ctaaattcat cggatgaaaa ccgtctacgt ctttttcagg gcggatagct
     6421 aacaggatct tttcttcatc tatatgcttt ggcaatggta attggaccaa tataccatga
     6481 aaactaggat cttgattgta tttttcaatt tctgcaagta gttcgtcttc agaaatcgtc
     6541 tcaggataac gctctaccaa tgaatggatt ccgatttctt ttgctacacg ttctttattt
     6601 cttacataga tttggctggc tggattttcc cctaccaata aaacgactaa tccgggatgg
     6661 ataccattat ctttcaattt ttggattttt tcagcaatct ctgcctgcat tttttcagct
     6721 aattcttttc cgttaatcaa ttctgccatc taaccactcc cagatatttt ttgtgttttc
     6781 attctaccat agaattttcc cgatttataa caggtttatg atgattcata gaaaaaagct
     6841 aggacttacg ccccagcttc taattctttc aatacattgg ataaaacacc gttgacgaat
     6901 ttacgtgaac ggtcatcact aaatgttttt gccaactcga ttgcttcatt taaagcaaca
     6961 gccgctggta catctgtcac ataagtcatt tcaaaaatcg cgatacgcaa gatcaccaaa
     7021 tccatttttg agatccgatt gatgtgccaa ttgtttttta aatgtttttc aatcacttta
     7081 tccaattcat ctttttttgc acagacaccg ccaactagcg tatcaagata aacaggaaca
     7141 aactctgatt catcttcgtt gatcatgtcg cggtggtcta attcgattgc gttgaaaatc
     7201 gcatcttctt ttgttaggtc tgcattgaag tctaatggaa ataaagcctg caatgccatt
     7261 tcccgaatct cgtgtcttga taattcttta ttcattctct tcctcttcgt cttcaaaaag
     7321 ttcttcgatt gttggttgtt ctaatttttc aggaacaaca gctactacat ggatattgac
     7381 ttcagataaa tcgatatccg tcatgaaaag gacttgctgg ctgacccgtt cctgcatttc
     7441 cattgctact ttaggaactg aaacaccata ttctagataa cagtaaagat ctactttcaa
     7501 gccatcttcc tcgtttgcta agtaaacacc ttttccgtga gctgcacgtc ctaataattc
     7561 agtgacattg ctggcaaaag ttccgcgcat tccgtatacg ccttctactt tagaagctgc
     7621 aatcccgata attacttcga tgacttcagg agcgatcacg atctctccta aatcttggtt
     7681 tgcgtttaaa atcaaatttt tttcatctgc catttattta ttcctccctt tgcctaagca
     7741 cgagatacat ccgaattctc tatgatgcgg catagtgatc acgtggtaag aaaactgccg
     7801 ccgatcagct gcaaactgct atgaattgga gaaaacaccc aactttatta tctcttacag
     7861 cttaaaagtt cttctatagt gtatcattaa atcgagtgat tttctataac aaagtctgtg
     7921 aacactgatt tttacgctta cttcttctat tgatgaaact ataagatgat caattctttt
     7981 ggtgaacgag tcaacacacg atttccatct tctgtgatca acaagtcatc ctcaatacgt
     8041 actccaccga ttcctggtaa gtagatccct ggttcatcag tgatcacatt tcctggtaca
     8101 aattgctttt ctgcacgaga agaaacgttt ggtccttcgt ggatctctaa accgatccca
     8161 tgtcctgtag aatgtccaaa tgcttccccg taaccatgtt ttgaaatata atctctagca
     8221 actgcatcta attggacacc agtcattcct ggttttgcag catcaataac tgctaattga
     8281 gcatctaaga ctatttgata aatttctttt aatttttccc caggatcacc aatagcgaat
     8341 gtacgtgtca tatcggaaac gtacccttcg taatagcaac caaagtccaa tgtgatcaag
     8401 tcaccttgtt cgatgatttt tttgcttgct acaccatgcg gcatagccga acgcaatccg
     8461 ctagcaacga tcgtatcaaa agaaacacct gaagcgccaa gagaacgcat ataaaagtct
     8521 agttgatttg cgacttcaat ctcagtcatg cccggttgga tcattttcag gatgtgatca
     8581 tatgccatat ccgcaatatg acatgctttt tcgataatag cgatttcttc ttcatctttc
     8641 acttctctga gttcttcgat catctgtgaa acaggtacta attccgcatc gatgatttct
     8701 tctaaaaccg aatattctaa aaatgagact gttgtttcct caaaagctaa tgagtgcagt
     8761 tcttctttgc gtactaagtt tgctacctca tcaaaaatag gtccgacatt tttaataatt
     8821 tcaaaccctt gagcctgcgc tgccgcctgc tcggtataac gaaaatcagt aataaaaaat
     8881 gctttttcca aagtaataac agctaatcct gtcgtacctg taaagttcgt taagtaacga
     8941 agattgtacg gactggtaac caaaaatgaa tccaaatttt cttcttgcat ttttttgcgc
     9001 aatttttcta cacgtagcat cataatgaaa ctccctcttt ctttgcacgc ttttttccat
     9061 tatagcaaac ttttcttcgt acacgacagt taaataaaaa tttccggaaa acttcttttt
     9121 attttaaata aataaagcca aaaaggagtc acagtctcgc tgcaactcct tttagtttac
     9181 tattttgtaa aaacttatga tcgttttcca aagaaaaatg agacaactgc taccaaaatc
     9241 actgcgccta aaatagatgg aataagtgcc atacctgcag cttttggtcc ccattcgccg
     9301 aaaatcgctt gtccaatact tgaaccgact agtccagcaa tgatattcgt tacacagccc
     9361 atcgaatttc ctttactggt gatcgcacct gcaattgctc cgatcacggc tccaacaatc
     9421 aatgtccata accaactcat aatataacct ccttctttgc taatcaattt tagcatatgg
     9481 gatttaaagg ggacaaataa ttggttttgc tatttcatat tttcttctgc aaagtaaaaa
     9541 aataaagaaa acctcctctt agaagttttc tttatttttt acttgcgttt aaacatacta
     9601 cgaataccac gtttcactac atcaaagaaa ctcaatgatt gtaccattct atcttcatct
     9661 acatattctc tgatcgcacg aagcactttt ttaggttctt tggaagaaaa tgtgtatgta
     9721 ccattttttt tcgtttgaat ggcatatcga ggaatccatt ttccctttag catgactgat
     9781 gctagtacat aatccacttc ttcccatgga atttggatat atttgcgtct atctcgcgca
     9841 tggtaaaact caaaaccatt atcacctatc atgatttgcc cgtaatctgt cagacccgta
     9901 aacgaagtag catcaattac caagtctact tttgtattga ttgattgaac cattttctcc
     9961 ctccagatat ttgtttatga acagtatact atataaaaaa agctcgggac aacgcccgag
    10021 ctatattttt atggattata aaagtccgat gacgtgtcca acaacaccga ctacgaataa
    10081 accaaggatg ataacgattg gagaaacctt tttcttaagc aaccacatac ataacaatgt
    10141 taatcctaat gcagctaaac caggaatcaa ttggtctaag ttgtcttgta atgttgttac
    10201 cttttcagga gaaagggcta agccattatt aacttgttca aatgcactct tgattccttc
    10261 accacctgaa ggcaatttat cccattcgat gtaagcacct ttatctaatt ttacagttga
    10321 tacaactggt aagaatttga ttgataccca tctttgtaca agggcagcta atacgaacat
    10381 ccctaaaatt gaagcacctt ttgttacgtc ttgtaacaat ccgccagaaa ggtcatcagt
    10441 aattttagaa ccagctttgt agccaaattc ttgtgtatac cacatgaatg cccaacgaat
    10501 aatgttccaa cctaagaaga agatgattgg tccaaggata tttcctccca tagctaatga
    10561 agcacctagt gcacctagca ttggacgtac tgtaaaccag aacactgggt caccaacacc
    10621 agctaaagga cccatcatac ctactttaac cccttgaatc gctacatcat caacaggcgc
    10681 gccattcgca cgttcttctt ccagagccaa tgtaacccct aaaattggag aagcaatgta
    10741 tgggtgtgta ttaaagaatt ccaagtgacg ttttaatgct gcagaacgtt cttcttttgt
    10801 tttgtataat tttttgattg ctggaatcat agaaaatgcc cagccaccgt tttgcatacg
    10861 ttcgtagttc catgaacctt gaatgaaggt agagcgccac gcaacagcta aacgatcttt
    10921 ttttgataat ttgatttttt cttctgccat tttattttcc tccttcaaga ttaatagtca
    10981 ttcaaaatgt cgcctaaagg gtcaccagag ttgcttccgc ctccattatt tgatgaacca
    11041 cccattttag aaaggttcaa gtaaataaga gctaatgcaa cacctaaagc acctagagca
    11101 atcaatgtta attgagaaat tgcagctaca acgaaaccaa taacgaagaa tggccatact
    11161 tcttttgtag ccatcatgtt gattaccaaa gcgtaaccta cagctacgac cattccccca
    11221 ccgatagcca taccatctgt taaccatgct ggcattgatt caaggaatga ttgcacagtt
    11281 tgtgctggga taaatagcaa cgctgctgct gggaccgcga tacgaacccc ttgtaaacat
    11341 acagcaatga tgtgccacat ttcaacctta cggatgtttc cttcttcagc agccgcatcc
    11401 atcaaatgaa cgattggaac agcgattgta cgtacgatca ttgttaagaa caaccctgca
    11461 acagccaaag gaacggcaat cgcgatcgca gaaggaacac ctttaacacc ttgtccacct
    11521 aaaactaaaa taattgctga tgctactgat gctaatgcag catctggtgc tacagcggca
    11581 ccgatatttg cccaacctaa agcgatcatc tgaagcgtac cgccaagtac gataccagct
    11641 tcgaggttac cggtaactaa accgatcaat gtacatgcca ctagtggttg atgaaattga
    11701 aattcatcaa gaataccttc cataccagct agaaaggcaa ctaatacgac taaaattatt
    11761 gaaataatag acatgataag cctcctataa attttaatat tatttattac gcttttgcta
    11821 attcagcttt tgcttttttc aaaatatcat ccatgttatc gcgtgaatca ttaggtactt
    11881 tacgtacatc gaatttcaca cctttttgtt caagcttctc aaacgcttca acgtcttctt
    11941 gtcccataga caatacttta cttacaacga ctttaccgac cgaatgcgcc attgacccaa
    12001 catttaattc tttgatgtct acgccacctt caacagctgt caatacgtct tcaggatttt
    12061 caaataaaag caatgctttg gtatttccaa aacgagggtc ttttgctact tcgatcattt
    12121 tgctaatagg aataacattt gcttttacac ctggaggagc agcttgttcg attagtctct
    12181 tacgcagatc atcctttgat actgcatctg aaacaacgat aatacggttt ggttgcactg
    12241 cttttgtcca agctgtcgca acttgtccat gtaacagacg tgagtcaaca cgtgctaata
    12301 catatttgat ttttccgtct ccgaccacag ttccttctgg aagtgctcct ttaggttgtg
    12361 cgtcttcaac tgctgctttc ggtgcttctg ccggttctaa ttcttccggt tttactttta
    12421 caccgtcttt tgctgtttca atgatgtgtg ctgcaatttc atgagcagat tccattgaaa
    12481 agcgtgatgc gtaggcttca atcaacattg gtaaattcaa accgctgacg attgcccatt
    12541 tatctttgtg ctcttcgaat aatgtgtttg attgattgaa cggtgttccg ccccataaat
    12601 caactaagaa taacacttcg tcttgattat caaaagaagc aaccgcttct tccaatttgg
    12661 ctctcaagtc atctggacct tcacttggtt tcaaaatcac ggctttgacg ttttcttgtt
    12721 cgccaaagat cattgaaccg gattgtaaga ttccttcagc aaattgtcca tgacttgcta
    12781 ggataatccc taccatattt atacctccta tttacttttg ttgtaacaaa ttcaaaaata
    12841 ttaataattg ctaaccatta caacagaatt atttgacctt ttccaacaac cattcttgct
    12901 acaccttttt gattttcgct agtagttcca tcaaaaggat tttcctgtca gcaggtactt
    12961 ttcgaatctc taactccact tcttgactgt tcaagtaacg aaaggaatca atatcttgtt
    13021 gatttactga aacaaaattg gtaatcattg atttgccttc agtaaaactc ataccgccga
    13081 tattcaacga cttcaactgt acaccgcagc ggacaaccgc ttcaacatct tggggattgg
    13141 tgaataaaag catcactttt gctcgtgata ataatccact atgatagatt tgaatcattt
    13201 tctgcacggt tattacattt gctgtgatcc ccggcggtgc agcttctttt agtagaaact
    13261 ttctcaattt atcttgagcg actacatcgc tgatcacaat gatacgttca acacctgttt
    13321 gcttcgacca aactgttgcg acttgcccgt ggattaaacg atcatctata cgtgccaatc
    13381 gaatatccat ctgcaaaact ccttcttcct cactacttca cagaaacccc tatcatttct
    13441 gtgtgaacaa ccacctccta gcacacacta ctataagcaa aaagcgtgcc aactttttta
    13501 cgatacacta attgaattta aaggattctt aaccaataca caaaaacgat acactataaa
    13561 agtgtatcgt ttttgtgtat tcatggcaca gtgtattctt ttttatctaa ctgattatgg
    13621 actagacgta gcagataata catttcttct atcggcactt ttacttttat ctgatcttcc
    13681 aacagaacaa ctgaggctgc caattggcta taaagaggat cattcgttgt atttgtcaat
    13741 tcgttttctt ccacagtcaa tggttcattc cgaataatac gttcgatcat tcctgctgtg
    13801 tgcatcacta ggttgatttt gaatccatat tcttcatcgc cgattcccgt cgtctgagag
    13861 atttgtgtag aaaattgcca cattggatcg attaacttac taccattaat aaagatgaag
    13921 ttatcagaga taaactcaac acacgtattt ttagctgact cttcatctag ggagatgaat
    13981 tcagtttcct ccaattctga ttccatcagc aactgatcca aaatgacttc gatattttga
    14041 tcaataaaac gttctaaagg aataaatggg gcttggatat tcaaatcagt aatcccagta
    14101 gtagctatga tctgatattc tttttggata actggcagtt cttctttcac ttccacaatc
    14161 gacaaagcta gaacttctaa ttctgtttct tgacgcttgt ttaccgctcg ttcgatcaat
    14221 tctttgattc tttgtgctgt accttcgcca gatgcgcaga tagccaaaac agcttttttc
    14281 ttatgaatag gttttgcttc ctgaacagat tctacagaag atatttctgc gtaacctcga
    14341 aaatttttca atgattcatg caacatatct agatctgtcc ctaatactga gacctttcgt
    14401 acagtttcca aaactagaga agttgttacc atatcaacag tacgtactgg aattccagtg
    14461 tctctttgaa tttgcgaatt aaacgacgca agggacccca tatctactaa gagtatgacc
    14521 ccgcttcctt catctacttc ttgtacattg cgttcaattc ggtccaatgc tgttttcgga
    14581 tccatatcca aaggcatgtc tactgctcgt acgttctctg catctaacaa ctgttggaca
    14641 acttggacca tactgcttgc tgtgctgttt ccgtgagctg caacgactac accgattctg
    14701 ccgcttgctt gatttgcccg taacgagacg agtagaacgg tcaagtaata gtcttcactt
    14761 tctggaatct ccacttgaaa atacgtttcg atataactac gcacttcttt cgcaacagca
    14821 tattcatctg gaaaatcaaa agccatctca cggatgttat cattcgtatg ccgttcttct
    14881 cctaagttga tttttttcaa aaaagaactg atatgcaagc tcatagcata aataaagttt
    14941 tgctgaaact catatggtaa agatcttttg accattacat aaatctgatt ggtcacttct
    15001 atgattttcg gatctacaaa ttctgctaat ttgctgtctg cattaaaaga aaaaccatgg
    15061 tctttataga aagacttcag atggatgttg atatctgtag aaatgaaatg attgattgct
    15121 tcttgatcca aaccatccga cttcagtaaa gctgctttat ctccgatgat atcatataga
    15181 ttataaggta actcatagga gtccgattgg attttcatga tcgtatcatt tggtgctaca
    15241 gttatctttg gctccaatat tttagaaagc tctgccattg cactgcggtt gcttgctaat
    15301 tgaatcaatc cgctacgaat cccttctgtc aaatcatcga ttgtgatcga gatttcgggt
    15361 gattgcatat gatttagaaa tcctcgtgca catactaact ggatatttga cttcaattgt
    15421 cctatatttc cataggtcac actgccgatc aatgcttttg ctacatcttc tgtcaaagaa
    15481 atctttcgct gcgtgcggtt tgcttcgtgg gcaatcatga tttttactaa atcgactttc
    15541 tcatttgcag gacgtttttc aaaagccgga agtttgatat tgattgggat acgccgaaca
    15601 aaagtttcta atagagaaga acctgggtct tctgtggtcg cacctacgat ccgtacattt
    15661 gcttcatggg atttcgttgt ttccccaaga cgactgtaag ttccatgatc cataaagtaa
    15721 aagatcattt cttgaccttc aggcggcaac cggtgaatct catccaaaaa cagcataccg
    15781 ccatccgctt gatcgataat ccccattttt tcttcatctg cgcctgtaaa agcacctttt
    15841 gcgtacccaa ataaatgact catcaaaagc tcagggttat tggcataatc agcacaattg
    15901 aaaacaatca gttccgtttc ctcatctatt acatgatgcg cttttgcaaa atgatacatc
    15961 gcgtgtgcaa aataagtctt tcctgatccg gtaggtcctg taatcaaaca atttaatcct
    16021 cttggcggat acaaaatcgc agcttttgcc tgttccactg aatttttcat gctgccgtta
    16081 gctccaatga tttttgcaaa gatatcctta gtatctattt ggagtgtatt tttttcttca
    16141 gagaccggcg tttctttgta actgactaca tgttttgtaa ctggctttac gtgcgtaacg
    16201 agtgtttgat atatgtaacg tactggtcgt ccgtctgtct ttatcagttt tccttcgcgc
    16261 actagctgat tcagatcttt actgctattt gttcgctgaa tccctaacac ttcagcaatt
    16321 tcctgtgttg ttactccttg atcatattct gcctttgtta aatgagcggt tttttctttg
    16381 acatacatat aaattcggtc aatgcgtttt gccaatggta tcgctccttt tctatagact
    16441 attgtactta ggcttcaaaa cataatacta tgtatttatt tctgtttcgt caactattta
    16501 cctgattttg ttttgttgat aacacgcgga tagatagaaa tgttcaattt aaaagaacga
    16561 aaaatcccga tacgattttt at
//
LOCUS       DACDKL010000048        16500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDKL010000048 DACDKL010000000
VERSION     DACDKL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.048"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..550)
                     /locus_tag="IV329_001191"
     CDS             complement(152..550)
                     /locus_tag="IV329_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8940384.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="IV329_001192"
     CDS             complement(547..1320)
                     /locus_tag="IV329_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8940385.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="IV329_001193"
     CDS             complement(1317..2000)
                     /locus_tag="IV329_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8940386.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="IV329_001194"
     CDS             complement(2167..3054)
                     /locus_tag="IV329_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8940387.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="IV329_001195"
     CDS             complement(3051..3920)
                     /locus_tag="IV329_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8940388.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="IV329_001196"
     CDS             complement(4057..5343)
                     /locus_tag="IV329_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8940389.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="IV329_001197"
     CDS             5616..6194
                     /locus_tag="IV329_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8940390.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="IV329_001198"
     CDS             6194..7123
                     /locus_tag="IV329_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8940391.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="IV329_001199"
     CDS             7120..8856
                     /locus_tag="IV329_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8940392.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IV329_001200"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IV329_001200"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8940393.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IV329_001201"
     CDS             complement(9752..10261)
                     /locus_tag="IV329_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8940394.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IV329_001202"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IV329_001202"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8940395.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IV329_001203"
     CDS             complement(11562..12653)
                     /locus_tag="IV329_001203"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8940396.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IV329_001204"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IV329_001204"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8940397.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IV329_001205"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IV329_001205"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8940398.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IV329_001206"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IV329_001206"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8940399.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4770 a         3634 c         2926 g         5170 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
//
LOCUS       DACDKL010000049        16351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDKL010000049 DACDKL010000000
VERSION     DACDKL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.049"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(181..1116)
                     /gene="murB"
                     /locus_tag="IV329_001207"
     CDS             complement(181..1116)
                     /gene="murB"
                     /locus_tag="IV329_001207"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8940400.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1189..2109)
                     /locus_tag="IV329_001208"
     CDS             complement(1189..2109)
                     /locus_tag="IV329_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8940401.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2222..2974
                     /gene="xth"
                     /locus_tag="IV329_001209"
     CDS             2222..2974
                     /gene="xth"
                     /locus_tag="IV329_001209"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8940402.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3838..4371
                     /locus_tag="IV329_001210"
     CDS             3838..4371
                     /locus_tag="IV329_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8940403.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4861..5385)
                     /locus_tag="IV329_001211"
     CDS             complement(4861..5385)
                     /locus_tag="IV329_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8940404.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5378..5851)
                     /gene="tsaE"
                     /locus_tag="IV329_001212"
     CDS             complement(5378..5851)
                     /gene="tsaE"
                     /locus_tag="IV329_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8940405.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5974..6954)
                     /gene="pta"
                     /locus_tag="IV329_001213"
     CDS             complement(5974..6954)
                     /gene="pta"
                     /locus_tag="IV329_001213"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8940406.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7107..7787)
                     /locus_tag="IV329_001214"
     CDS             complement(7107..7787)
                     /locus_tag="IV329_001214"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8940407.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7913..8782
                     /locus_tag="IV329_001215"
     CDS             7913..8782
                     /locus_tag="IV329_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8940408.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8808..9323
                     /locus_tag="IV329_001216"
     CDS             8808..9323
                     /locus_tag="IV329_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940409.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9370..9933)
                     /locus_tag="IV329_001217"
     CDS             complement(9370..9933)
                     /locus_tag="IV329_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8940410.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9988..10167)
                     /locus_tag="IV329_001218"
     CDS             complement(9988..10167)
                     /locus_tag="IV329_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940411.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10244..10753)
                     /locus_tag="IV329_001219"
     CDS             complement(10244..10753)
                     /locus_tag="IV329_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940412.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10849..11229)
                     /locus_tag="IV329_001220"
     CDS             complement(10849..11229)
                     /locus_tag="IV329_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8940413.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11559..12308
                     /locus_tag="IV329_001221"
     CDS             11559..12308
                     /locus_tag="IV329_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8940414.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12492..13769)
                     /locus_tag="IV329_001222"
     CDS             complement(12492..13769)
                     /locus_tag="IV329_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940415.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13848..14492)
                     /gene="pcp"
                     /locus_tag="IV329_001223"
     CDS             complement(13848..14492)
                     /gene="pcp"
                     /locus_tag="IV329_001223"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8940416.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14691..15635)
                     /locus_tag="IV329_001224"
     CDS             complement(14691..15635)
                     /locus_tag="IV329_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8940417.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15637..16323)
                     /locus_tag="IV329_001225"
     CDS             complement(15637..16323)
                     /locus_tag="IV329_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8940418.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4709 a         3382 c         2890 g         5370 t
ORIGIN      
        1 ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct
       61 atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca
      121 caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta
      181 ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac
      241 ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa
      301 ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc
      361 agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc
      421 tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg
      481 ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc
      541 tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg
      601 acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa
      661 aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata
      721 aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc
      781 tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt
      841 tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc
      901 atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg
      961 gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc
     1021 tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat
     1081 cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca
     1141 aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg
     1201 acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg
     1261 tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat
     1321 tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt
     1381 tccatcagcc tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt
     1441 acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc
     1501 cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct
     1561 tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag
     1621 atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt
     1681 ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa
     1741 gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc
     1801 acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc
     1861 tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc
     1921 gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt
     1981 ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg
     2041 ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc
     2101 aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac cattttttac
     2161 taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt
     2221 attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt
     2281 agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc
     2341 aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa
     2401 aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat
     2461 gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt
     2521 tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt
     2581 agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat
     2641 cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa
     2701 tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa
     2761 cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg
     2821 gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac
     2881 gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag
     2941 tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt
     3001 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata
     3061 ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc
     3121 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct
     3181 tattaccgca gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac
     3241 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
     3301 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
     3361 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
     3421 tggacttatt accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca
     3481 gcgtcctgac ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt
     3541 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa
     3601 agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat
     3661 aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga
     3721 gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa
     3781 tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg
     3841 aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt
     3901 gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct
     3961 gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc
     4021 aatgcgccga aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt
     4081 ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat
     4141 tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg
     4201 tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta
     4261 gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa
     4321 gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact
     4381 tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt
     4441 tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg
     4501 agaacaccgt ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag
     4561 ccaaacaaaa atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag
     4621 cttttggaca ccgtttacca agaggttgtg aaatcagcgt tttgacctga gtattagaga
     4681 aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
     4741 ctgattgaac accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc
     4801 ttcgtgtgtt tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt
     4861 ctattcaatc aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc
     4921 ccctctaggc atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct
     4981 ttcattacga acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca
     5041 actgatgatt tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat
     5101 actgataccg acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc
     5161 tgttccaata atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta
     5221 tatataatcc aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa
     5281 aactaaaaat tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc
     5341 tcctggcact gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt
     5401 tttctagaat tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat
     5461 accgcttttc taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca
     5521 gtgcttcttc taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt
     5581 catctaaccc taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg
     5641 gaatccgacc ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg
     5701 ttatccctaa tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac
     5761 cagtcaatat cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt
     5821 ctgtcattgt taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt
     5881 ttgccagtat atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc
     5941 agctgctgtt tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt
     6001 gataatcgat aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg
     6061 tttgttcaag ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat
     6121 tttatagccg atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc
     6181 tacttctgaa tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg
     6241 caattcgccg tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac
     6301 tactttgtct acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac
     6361 ttttggttcg atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa
     6421 ttcttgcgca gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc
     6481 tcttccacgt accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat
     6541 gatctgaagt gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc
     6601 gtctgcaatc cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt
     6661 ttctgcttgt tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc
     6721 atcccactta tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct
     6781 ggcatgcgct gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc
     6841 ttctgctttc aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac
     6901 gattttgatc ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg
     6961 acctccagac gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat
     7021 tttatctgta tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg
     7081 cactataaca ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt
     7141 tcttctccta acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag
     7201 ccacgacttg cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta
     7261 tcgatcattt tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt
     7321 tcccgttcat ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga
     7381 tgagaatacg cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt
     7441 ttagcccatg acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc
     7501 tctttataga tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat
     7561 aagccatgag cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact
     7621 tcttcataag gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt
     7681 tgggacgcat attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat
     7741 tctgaggaca aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc
     7801 taataaaaag aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac
     7861 ttgattaata tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa
     7921 attaattgca tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga
     7981 aaatgccgag gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg
     8041 tcgaaactat caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat
     8101 cactttgaac ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat
     8161 tccaaataat caagcaatct ctgttttaga tattctagat gctaacggta tttactatga
     8221 agtagcaacc aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc
     8281 ttccatggtg gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc
     8341 agctaagctg gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga
     8401 aaagaatctg gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg
     8461 tccagttgga aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa
     8521 caatatcgaa gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca
     8581 cgagcgtggg atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag
     8641 catgctgcaa gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga
     8701 ttatgccaaa tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg
     8761 cgcgattgca gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt
     8821 tcattcagga aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac
     8881 attccctgtt tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg
     8941 ccatgaatgg cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt
     9001 ttgagctgta taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg
     9061 gtgactttta ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc
     9121 aatatcaaat ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt
     9181 caggagatta ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta
     9241 ttgctgcggt tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt
     9301 cttaccgact taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg
     9361 tacatctttt tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg
     9421 accgacaact ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc
     9481 cacacttaga ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa
     9541 taatcgcgaa ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata
     9601 ccattcgttg ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata
     9661 tttctctaag ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc
     9721 gtaagcaact cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta
     9781 acgataattt ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt
     9841 ctgcttcaaa aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc
     9901 tggagcatct tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa
     9961 catgcagaaa aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat
    10021 gaaataacca tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg
    10081 catttcttta tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc
    10141 gcaattaggg catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt
    10201 agcttactct tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata
    10261 cctgacggtc ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga
    10321 ctagaagtgt ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca
    10381 tcgctgcttg cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg
    10441 ccttttccgt gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt
    10501 gtccttcaca aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt
    10561 caactgcaaa atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg
    10621 ctgctttttt caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg
    10681 cattgagcag tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca
    10741 tcattttttt cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac
    10801 gaatgaaaaa tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag
    10861 ttctaatggc aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc
    10921 ttctctaatc ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat
    10981 atcgtctact ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg
    11041 tggcgcatta gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt
    11101 atagtcatct cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac
    11161 ataaaaatca cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt
    11221 aaggttcata tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt
    11281 ccattgtaaa tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg
    11341 caaaaaaaac agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga
    11401 aagtaagaaa ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta
    11461 taattgaaag actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca
    11521 gaacacacta catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt
    11581 tagctcatca gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg
    11641 gctgccaggt catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta
    11701 tcggtaccac gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg
    11761 gtattttagg tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact
    11821 atttaagtat cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa
    11881 gtaaagaatg gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt
    11941 ttatcgcaat catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt
    12001 atacagatga tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg
    12061 gtatcatcag tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg
    12121 ctcaaatggt cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg
    12181 tgaacagctc tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata
    12241 accaaaaaga acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac
    12301 acgcataaaa aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    12361 cacgtataaa cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    12421 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    12481 gcctctattt tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt
    12541 gattcttcta acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa
    12601 tgttttcctt tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact
    12661 actagttgcc atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct
    12721 tctcgttctt tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg
    12781 aagaaccctt ctgtaaaacc atagcgacaa tattgcttcc aaataaatag atagattttt
    12841 ggttggatga atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt
    12901 cgttgacgtt ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt
    12961 actggcactt cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg
    13021 atcaagaatt cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt
    13081 tttatctgtc gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt
    13141 tccaatgctt gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc
    13201 cgctgagcta attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag
    13261 acaaaacagt atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat
    13321 aataattcgt ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact
    13381 gtcggagaga catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg
    13441 gtttttggcg atttaacgat gtattgcact tttaattctc taccacactc acaatacaat
    13501 ttttgctggc ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa
    13561 tctgttgcag ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc
    13621 ttatcgacta gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg
    13681 cttgtgtatt gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat
    13741 tcttcaaaga cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag
    13801 ctggctacta acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt
    13861 ccggcatttc cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa
    13921 gaagaagcaa tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga
    13981 tcgggtgaga atggcacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc
    14041 aaatgaagca aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg
    14101 tacgctggta gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta
    14161 aagtaagcgg ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta
    14221 tcagggattc ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc
    14281 ccacctgctt gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg
    14341 actacttgac tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg
    14401 ctgatttcat ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca
    14461 aatggatcaa aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa
    14521 catatggtta tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag
    14581 tttaaataac ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac
    14641 aatgttagaa gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat
    14701 agaatcatat aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg
    14761 atcactccgt atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca
    14821 gccatcggtg tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac
    14881 gctggattcg ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg
    14941 atggccgcaa atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg
    15001 atgatgccga tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact
    15061 gtcccaactc cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg
    15121 cttaacggcc ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga
    15181 ttatctcgag aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg
    15241 actaaagcac ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa
    15301 gctggaataa aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat
    15361 ggaagccggc tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag
    15421 atcaagccac cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag
    15481 accgtccaga aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt
    15541 actccagtgt aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg
    15601 cctaataaaa tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata
    15661 tttcttcgct aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt
    15721 gatagccact ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc
    15781 tttcagtgtt gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa
    15841 gaaccagcag acattttcca tagctgaagc catacctttt agttcttctt catgcttagg
    15901 atggacttct aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg
    15961 aacaaatcca gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat
    16021 cccataaata tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc
    16081 cgctgattct ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa
    16141 aatcgccatc ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat
    16201 gccaagaccg ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc
    16261 taatttaaat gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc
    16321 catatatttt cctccataaa atgacaatat t
//
LOCUS       DACDKL010000050        15989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDKL010000050 DACDKL010000000
VERSION     DACDKL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.050"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            49..705
                     /locus_tag="IV329_001226"
     CDS             49..705
                     /locus_tag="IV329_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8940419.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1147..1944)
                     /locus_tag="IV329_001227"
     CDS             complement(1147..1944)
                     /locus_tag="IV329_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8940420.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1941..3125)
                     /locus_tag="IV329_001228"
     CDS             complement(1941..3125)
                     /locus_tag="IV329_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8940421.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3150..4397)
                     /locus_tag="IV329_001229"
     CDS             complement(3150..4397)
                     /locus_tag="IV329_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8940422.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4612..6357
                     /gene="dnaX"
                     /locus_tag="IV329_001230"
     CDS             4612..6357
                     /gene="dnaX"
                     /locus_tag="IV329_001230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8940423.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6377..6691
                     /locus_tag="IV329_001231"
     CDS             6377..6691
                     /locus_tag="IV329_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8940424.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6716..7312
                     /gene="recR"
                     /locus_tag="IV329_001232"
     CDS             6716..7312
                     /gene="recR"
                     /locus_tag="IV329_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8940425.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7376..8020
                     /locus_tag="IV329_001233"
     CDS             7376..8020
                     /locus_tag="IV329_001233"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8940426.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8035..8364
                     /locus_tag="IV329_001234"
     CDS             8035..8364
                     /locus_tag="IV329_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940427.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8378..9316
                     /gene="holB"
                     /locus_tag="IV329_001235"
     CDS             8378..9316
                     /gene="holB"
                     /locus_tag="IV329_001235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8940428.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9352..10176
                     /locus_tag="IV329_001236"
     CDS             9352..10176
                     /locus_tag="IV329_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8940429.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10169..10516
                     /locus_tag="IV329_001237"
     CDS             10169..10516
                     /locus_tag="IV329_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8940430.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10585..11457
                     /gene="rsmI"
                     /locus_tag="IV329_001238"
     CDS             10585..11457
                     /gene="rsmI"
                     /locus_tag="IV329_001238"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8940431.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11566..12687
                     /gene="dinB"
                     /locus_tag="IV329_001239"
     CDS             11566..12687
                     /gene="dinB"
                     /locus_tag="IV329_001239"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8940432.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12741..13343)
                     /gene="nrdG"
                     /locus_tag="IV329_001240"
     CDS             complement(12741..13343)
                     /gene="nrdG"
                     /locus_tag="IV329_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8940433.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13658..15847)
                     /gene="nrdD"
                     /locus_tag="IV329_001241"
     CDS             complement(13658..15847)
                     /gene="nrdD"
                     /locus_tag="IV329_001241"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8940434.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         4974 a         2981 c         3348 g         4686 t
ORIGIN      
        1 aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt ggatagtaga
       61 gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg atatcataaa
      121 tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga gggaacggta
      181 aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat caaagatctt
      241 gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc gtataatatt
      301 cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt ttggcaaaat
      361 atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta tcgtctgctt
      421 tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc tgtttgtacg
      481 caactttacg aattatatga tttattcggt gtgcggacag aagaagggat gctgatcgat
      541 ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc tagcagtgta
      601 aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga ccatcatatc
      661 gttattttga atatggactt gttagaagac aatattatct tttgatgaac aaagaggttg
      721 tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
      781 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta ccaagaggtt
      841 gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt
      901 ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt taccaagagg
      961 ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac agtctcttat
     1021 gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc gtttatttgc
     1081 aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac attcatttag
     1141 caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag aatgggtagt
     1201 acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg tcgtgacaaa
     1261 agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt ctcggaatgg
     1321 atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat aaaagacaag
     1381 actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca aaaccttata
     1441 gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa ctccgtcttc
     1501 cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg cagcatttgt
     1561 catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca aataatcgat
     1621 catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata gcaatactga
     1681 gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt ctcttggttc
     1741 ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt cattcagctt
     1801 ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag cctcatcatt
     1861 taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta gcaaaagaaa
     1921 gaataaaaaa aactttttca tcatatcaag taacctccga tcagaaagag gatcgctagc
     1981 ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg caggacaccg
     2041 ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact tgagtcattg
     2101 cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc gtttgtgatt
     2161 gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc ttttaacagc
     2221 ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc atattcgata
     2281 tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat tgctttatct
     2341 aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga taaggcattt
     2401 tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg cttcgtttct
     2461 gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc ctgcatggca
     2521 ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa cttatcgatt
     2581 tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca agcgagaaat
     2641 ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg caagaattct
     2701 ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata agtcgcagca
     2761 gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag atctatcgct
     2821 gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc gtcaaccgct
     2881 ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt aaaatcttcg
     2941 gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt ttgcttttgc
     3001 tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca atgaaatttt
     3061 tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg tgtaagaaca
     3121 tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt aaacggttta
     3181 ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc attgcattca
     3241 ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc tccgcagttg
     3301 gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt atccgcattt
     3361 ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc ttggttattt
     3421 tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac acccataaag
     3481 ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc aatggaacct
     3541 tgcacgtatc cttcacgtac gctaggatca cctagcatcg ccccttcgtt acgcatattg
     3601 atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga aacaatctcc
     3661 atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt gcttaattcc
     3721 aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg attgattgct
     3781 gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt gatttccact
     3841 tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac gacatgaata
     3901 gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc aaagtagttc
     3961 aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat ataaaaaact
     4021 tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc agcaattgct
     4081 tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt gtaataacct
     4141 tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat catgtttgga
     4201 tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg attggagcca
     4261 cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc actcatattg
     4321 tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat acttgttgct
     4381 gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa acaattattg
     4441 tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg aattgaaatg
     4501 gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt ttttttacct
     4561 aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct tatggcatat
     4621 caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg acaaaaagct
     4681 gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc ctacttgttt
     4741 acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa agcaatcaac
     4801 tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg atcaattact
     4861 gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg agtcgaagaa
     4921 atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta caaagtgtac
     4981 attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt gaaaacattg
     5041 gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca caaaatacca
     5101 gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac ggcagatatt
     5161 gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc agctgcattg
     5221 aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat tcttgatcag
     5281 gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt gacaggtagc
     5341 ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga agtaccagaa
     5401 gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg ctttctagag
     5461 gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa cctagttgag
     5521 gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt ttcagcggat
     5581 cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg ttttaccaat
     5641 agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc gtctgcttct
     5701 gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct acaacaagag
     5761 attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat tgagaataca
     5821 aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta tcgtgtaccg
     5881 acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa ccaagtaaaa
     5941 gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat gcttaaagca
     6001 agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta tgagattgtt
     6061 tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa cttaagccga
     6121 atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg gccagaactt
     6181 cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt tgcttctgaa
     6241 gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga agaaacactt
     6301 gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga tgattaataa
     6361 aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga tgaaacaagt
     6421 acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa cagagtttac
     6481 aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc gcatgaaaga
     6541 tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc aggatcttgt
     6601 cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga aaaaattagg
     6661 gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa tgaatatgca
     6721 atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag gaatcggtca
     6781 gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag tagtaaatga
     6841 atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta tctgcggaaa
     6901 catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc gaagcgttat
     6961 cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag aatatcacgg
     7021 actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc cagaagacat
     7081 caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag tgattatcgc
     7141 aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc tgatcaaacc
     7201 cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg atatcgagta
     7261 tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat aaatttctaa
     7321 ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca acaatgtgaa
     7381 cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca tcttgaatga
     7441 actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa gagaaccagg
     7501 aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa atgatcggat
     7561 ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc tagtcgaaaa
     7621 ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg tcgacagttc
     7681 tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc ggttgaatga
     7741 gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag attcagacac
     7801 cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag attcggaagg
     7861 tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag agaatccgga
     7921 aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa caagttatca
     7981 tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac gtttatgaaa
     8041 attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga attgattgat
     8101 gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc tggaaacagt
     8161 acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat catcaaaaaa
     8221 acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat ttcgatggat
     8281 gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt cgtcttacct
     8341 gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa taaacttggc
     8401 tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg ggcgtatcgc
     8461 tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag gtatatggct
     8521 tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa aatgcaacaa
     8581 ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac cggaaggaca
     8641 aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca gcggttacga
     8701 atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca gtgcggcaaa
     8761 cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt tagaaacaga
     8821 tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc attttcaaga
     8881 actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat tggaaaaagc
     8941 aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct ctcaagatga
     9001 atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc aaaagcaaga
     9061 tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag aaaaaacaca
     9121 acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc gtcaagcagt
     9181 agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac ggatattgat
     9241 cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac agttcgtttt
     9301 gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga aatggtagaa
     9361 gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc aggagactct
     9421 gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca tattgctact
     9481 gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat ccgtccgatc
     9541 ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa agatgcttat
     9601 gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat gaaaatggtc
     9661 aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc ggacgggcga
     9721 attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg tattgagctt
     9781 cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc atgtgggcgt
     9841 ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa aatggcaaaa
     9901 gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg cttgatgtgc
     9961 tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc tgactttgga
    10021 aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct gcttagccgt
    10081 atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg ggatgaattg
    10141 aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg acggattgaa
    10201 tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga agcatgcttt
    10261 gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc tgcgggaaca
    10321 cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc aggaactttc
    10381 taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct gctacgattt
    10441 atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag tgatctatcg
    10501 cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac tgctccagtc
    10561 tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg aaatccggta
    10621 tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat gagctacaga
    10681 agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg taatacacaa
    10741 aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga acataattat
    10801 aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat tgctcaagtc
    10861 agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt agcctgcata
    10921 aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac ggctttgatt
    10981 gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg taagaaaaaa
    11041 gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat tttttatgag
    11101 tcaccttatc ggatcactgc tacattgacg aattttttag aaatttttgg aaatcgccaa
    11161 gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg aagtacggaa
    11221 gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct gcttgtagaa
    11281 gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac gttaaaggaa
    11341 caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa agcagtagcg
    11401 ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt agactgagat
    11461 agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact tttcaaatct
    11521 cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat ggagctgcaa
    11581 tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga tgcttttttc
    11641 gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt aattgccaga
    11701 caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga agcaagaaaa
    11761 ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc gcaagccatc
    11821 tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg ggaagtattc
    11881 tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct tgatgtgacc
    11941 gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca agcagatatt
    12001 tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt tttggccaaa
    12061 ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca agacgctgtg
    12121 ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa aactgtacct
    12181 agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac agaaatgatg
    12241 ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag aggaatccat
    12301 gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca tacttatggg
    12361 cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc tgaaaaagta
    12421 gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa ggtcaggtac
    12481 acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta taaaaaagaa
    12541 gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga aaaaggaatc
    12601 cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa catcgtcctt
    12661 ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc ggaaatccgt
    12721 tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc ataggtttct
    12781 gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga tttttggacg
    12841 tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg tttgcttaat
    12901 tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt atcttctgaa
    12961 tcttgtagca attcatcaaa ggcatagcct gtccatgacc agatatcttt tgtcgtaccg
    13021 aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa aaaaggttcg
    13081 ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat gatttgttct
    13141 tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt gttgaagcag
    13201 ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc ttcaccatcg
    13261 acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt tgctaaccat
    13321 tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca aagatatagc
    13381 tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta aatccattgt
    13441 tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc catcgaaaga
    13501 gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt tcaaaagctt
    13561 gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt tgtttagcta
    13621 ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt ttcacacgtg
    13681 aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt cctaaatagc
    13741 cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat tctggacatt
    13801 cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag caatgatcga
    13861 ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat acagcttcta
    13921 aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat ccacctgaac
    13981 aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg tttttacgca
    14041 catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg atgatgccga
    14101 atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga gttgaataga
    14161 cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt aattctttga
    14221 cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca ccgaagaaag
    14281 ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga cggtttttga
    14341 ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagaacca tacatatata
    14401 aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag agaagcgcat
    14461 ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga tctccttttg
    14521 attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt cgtccgacat
    14581 ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag cccataggta
    14641 ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca ggatacattc
    14701 ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct tcagggttta
    14761 agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc cgcttttcac
    14821 tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt tcaaaccagt
    14881 tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa gtaaataagg
    14941 tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg gtttttgctt
    15001 ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct tttagatgtt
    15061 ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg gcagaacagc
    15121 caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct gtggcggtct
    15181 ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg tttagcatgc
    15241 ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa tccaaatcat
    15301 ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac attttcagtc
    15361 cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa acattactgt
    15421 ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt tcaatcgaaa
    15481 agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa ttgatgtact
    15541 cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg ttttgaatct
    15601 catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc aagtctgcaa
    15661 tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc ttttctgctt
    15721 tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca tcgcgtttga
    15781 tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct tcataattta
    15841 ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca aaatcgggga
    15901 aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt ggatttctat
    15961 actatatgta gtgtatattt tattgtgaa
//
LOCUS       DACDKL010000051        15988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDKL010000051 DACDKL010000000
VERSION     DACDKL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.051"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IV329_001242"
     CDS             complement(59..187)
                     /locus_tag="IV329_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8940435.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV329_001243"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV329_001243"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8940436.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IV329_001244"
     CDS             complement(1674..2729)
                     /locus_tag="IV329_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8940437.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV329_001245"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV329_001245"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8940438.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV329_001246"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV329_001246"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8940439.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV329_001247"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV329_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8940440.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV329_001248"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV329_001248"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8940441.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV329_001249"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV329_001249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8940442.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IV329_001250"
     CDS             10491..12050
                     /locus_tag="IV329_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8940443.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IV329_001251"
     CDS             complement(12161..14092)
                     /locus_tag="IV329_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8940444.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV329_001252"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV329_001252"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8940445.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IV329_001253"
     CDS             complement(15021..15773)
                     /locus_tag="IV329_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8940446.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4123 a         3499 c         2917 g         5449 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttct
//
LOCUS       DACDKL010000052        15942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDKL010000052 DACDKL010000000
VERSION     DACDKL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.052"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(378..800)
                     /locus_tag="IV329_001254"
     CDS             complement(378..800)
                     /locus_tag="IV329_001254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8940447.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(814..2220)
                     /gene="atpD"
                     /locus_tag="IV329_001255"
     CDS             complement(814..2220)
                     /gene="atpD"
                     /locus_tag="IV329_001255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8940448.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2243..3145)
                     /locus_tag="IV329_001256"
     CDS             complement(2243..3145)
                     /locus_tag="IV329_001256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8940449.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3159..4715)
                     /locus_tag="IV329_001257"
     CDS             complement(3159..4715)
                     /locus_tag="IV329_001257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8940450.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4738..5280)
                     /locus_tag="IV329_001258"
     CDS             complement(4738..5280)
                     /locus_tag="IV329_001258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8940451.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            complement(5267..5791)
                     /gene="atpF"
                     /locus_tag="IV329_001259"
     CDS             complement(5267..5791)
                     /gene="atpF"
                     /locus_tag="IV329_001259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8940452.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5882..6097)
                     /gene="atpE"
                     /locus_tag="IV329_001260"
     CDS             complement(5882..6097)
                     /gene="atpE"
                     /locus_tag="IV329_001260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8940453.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            complement(6150..6869)
                     /gene="atpB"
                     /locus_tag="IV329_001261"
     CDS             complement(6150..6869)
                     /gene="atpB"
                     /locus_tag="IV329_001261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8940454.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7171..10014)
                     /gene="rnr"
                     /locus_tag="IV329_001262"
                     /pseudo
     CDS             complement(7171..10014)
                     /gene="rnr"
                     /locus_tag="IV329_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10017..10763)
                     /locus_tag="IV329_001263"
     CDS             complement(10017..10763)
                     /locus_tag="IV329_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8940455.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10875..11111)
                     /gene="secG"
                     /locus_tag="IV329_001264"
     CDS             complement(10875..11111)
                     /gene="secG"
                     /locus_tag="IV329_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8940456.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11229..11507)
                     /locus_tag="IV329_001265"
     CDS             complement(11229..11507)
                     /locus_tag="IV329_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8940457.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11620..12588)
                     /locus_tag="IV329_001266"
     CDS             complement(11620..12588)
                     /locus_tag="IV329_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8940458.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12803..12847
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12871..13845
                     /locus_tag="IV329_001267"
     CDS             12871..13845
                     /locus_tag="IV329_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8940459.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13850..14425
                     /locus_tag="IV329_001268"
     CDS             13850..14425
                     /locus_tag="IV329_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8940460.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14492..15631)
                     /locus_tag="IV329_001269"
     CDS             complement(14492..15631)
                     /locus_tag="IV329_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8940461.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4337 a         3442 c         2873 g         5290 t
ORIGIN      
        1 atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt attgacaaag
       61 agccacaaaa gtcttgtcgc agtcccttat tccaaataaa cggtgttcaa tcatctgacc
      121 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttccattt gtttctctaa
      181 tactcaggac aaaacgctga tttcacaacc tccgatatac ggtgttccag aagtagcttc
      241 ttcgaaaaat aagctgagat ttcacaaaaa tttgagaagc aattttcgta aattccttct
      301 tatgtcttga agctgaccac ttctgtcata acctcttttt actgttctta aacacggata
      361 aacatccgca tctcatttta tgagtgctta gatactttga tacgattgat tgctcggtgc
      421 aacgcaacag ttgcccgttt caattcgttc gtatcttgtt ttgcttttgc ttcttcgatt
      481 tggcgttctg cgcgttgttt tgcacgttcc gcacgagata catcgatatc acgttcacgt
      541 tccgcactat cagcaacgat tgaaacaaca ttatcacgaa cttccatgat tccgccatta
      601 acagcgatcc aatcgacgtg ggtatctgaa tctgttcgtt ttacacgaac ttcatcaatc
      661 gtcaatggaa cgatgattgg tgcatgttta ggcaaaatcc caagctcacc agctgtcgta
      721 cgtgcaacga cgatagctgc atggtgatca tagaccaaac cggcaggagt taccacattt
      781 actgttaaat attgatccat ggggcacccc tttctagtag cctagtgttt tcgctttttc
      841 tacgacgtct tcgattcggc caacacttcg gaaagcttct tcaggaagat catcgtattt
      901 gccttccaag atttcgcgga atcctttcac tgtttcttct acagggacat atgaacctgg
      961 aagaccagta aattgttcag ccacgttaaa gttttgtgac aagaagaatt ggactctgcg
     1021 ggcacgagaa actaacactt tttcttgatc ggataattca tccataccta agatagcaat
     1081 gatatcttgt aattcacgat atcgttgcaa tacgtgttgg acttctgttg ctactttata
     1141 atgttcttct cccacgattt caggtgctaa agcacttgaa gaagaagcca atggatctac
     1201 cgctggataa atcccttgtt ctgttaattt acgttccaag ttggttgtcg catccaaatg
     1261 ggcgaatgct gttgccggcg ccgggtcagt gtagtcatcg gctggcacat agattgcttg
     1321 gatcgatgtg atcgaacctt ttttcgtaga tgtgatacgt tcttgcaatt gacccatttc
     1381 tgttgccaat gttggttgat aaccaaccgc agaaggcatc cgtcctaaca aggctgatac
     1441 ttcagatcca gcttgagtga aacggaaaat gttgtcgata aacaacagta catcttgacc
     1501 ttctacatca cggaaatatt ccgcaatcgt caaaccagtc aaagcaacac gcatacgtgc
     1561 acctggtggt tcgttcattt gtccgaacac catggctgtt ttttcgataa ctcctgaatc
     1621 tttcatttca taatacaagt cattaccttc acgagtacgt tcacctacac cggtaaatac
     1681 agaaatacca ccatgttctt gggcgatatt atggatcagt tcttggatca gtacggtttt
     1741 accaacaccg gcaccaccga atagtccgac tttcccacct tttaaatatg gggcaagcaa
     1801 atcgataact ttgatccctg tttccaaaat ttctgtactt gtacttaatt cgtcaaaggc
     1861 tggtgctttt ttatgaattt cgcttctttc cgcatcttct gggaaaggtg tttctaaatc
     1921 gatcgtatct cctagtacgt tgaatacgcg acctaatgtt tctttaccta caggaacaga
     1981 gattgctttg cctgtatcaa tgacttccat tcctctttgt aaaccatctg ttgattccat
     2041 cgcaatggtc cggatcactc cgtcccctaa ttctaacgct gcttcaagaa caacttttga
     2101 tttattttcg tcatttttat aaacaactaa tgcgttgttg atatctggta aggattggtc
     2161 taaagaaaat tccacgtcga caacgggacc gattacttga acaatcttgc ctgaactcat
     2221 cttttttcct ccaatctcaa tactattcta gtgccgaagc tccggcaaca atttcggtaa
     2281 tttcttgggt aatagcccct tgtcttgcac ggttgtaaga aaccgtcagc tcatcaatga
     2341 tattagcagc attatcagtt gctgttttca ttgctgtcat accagcagca tgttctgctg
     2401 ttttggcgtc aacgatcgca ccgtagatca aactttcagc atactgaggc aataactgag
     2461 acaagatttc ttcttttgaa ggttcaaaaa tatattcttg ttcgaaagta gttgcttctt
     2521 ctggatctaa gtctgagatt ggcagcattt tttctacacg aaactgacta gttagcgaat
     2581 tgatatgatg gttataacat acgtataatt catcgaatac ttcgttttgg tacatagttg
     2641 ttgccatatt tacgattttg cggacttcat caaagcttgg ctgatcagat agattgcgca
     2701 gctcatatgc tagattgata ccgcgtgctt taaagaagtc tgcaccagtt ccgccaattg
     2761 cgatcatcac gtaatcatcg ggtgattggt ggtcttcttc taaaatagac atcgtttgtt
     2821 tcaatatcga gctgttatat ccaccgacta aaccgccatc tgcagtgatg acgatataac
     2881 ccgttttctt aacaggacgg ctgagcaaca tgctgttgta attgatatcc cctctaggat
     2941 tttctgaggc aatatcattt aactgagtag cagtcaaatg cgtaacgatt tctcttactt
     3001 ttgttgcata aacctgaaac ttctgtgaag aagcttcaga ttttgtcaac ttagaagcag
     3061 acaccatctg cattgcccgc gtgatttggc tcgttttttt cgttgaagcg atacgtgtct
     3121 tgatttcatt taatgaagca cccattcatc tcacctcact atgcattttg aatggatttc
     3181 aatttatctt ctgtagagga attatttcct tttgtagcta agaacatgtc tttatattcc
     3241 tgaatcgcac tgttcaacgc ttcttcttct ggaagatctt ttgttgtacg gattgtttca
     3301 aagatatctg aatgattggt atccagatat tcgaataatt catgttcgaa gtctaaaata
     3361 ctgtctacag ggatactatc taagaatcca tgtgtcaaag catacaaaat cactacttgt
     3421 ttttcaacag ctagcggtgc atgcaatttt tgtttcaaga tttctactgt acggcgtcca
     3481 cgatttagtt ttgcttgtgt tgctgcatcc aagtcagaac cgaattgtgt aaatgcttct
     3541 aattcacggt aacttgctaa gtctagacgt aaagtcccag caactttttt cattgctttg
     3601 atttgcgcag aaccccctac acgagataca gatagtccgg catcaaccgc tggacgagta
     3661 cctgcgtaga acaagtcgct ttctaagaag atttgtccat cagtgatcga aataacgttt
     3721 gttgggatat aagcagagat atctccagct tgtgtttcaa caaatggtaa ggcagtcatt
     3781 gaaccgccac ctaaatcatc acttaatttt gctgcacgtt ccaataagcg tgagtgtaag
     3841 tagaaaacat cccctggata agcttcacga cctggtggac ggcgaagcag caaggaaagt
     3901 tcacgataag caacagcttg ttttgaaaga tcatcaaaaa tgatcaatac atgtttaccg
     3961 ttgtacatga attcttcacc cattgcagta ccagcatatg gtgcgatata aagcaatggt
     4021 gctggttgag acgcaccggc attcacaacg attgtgtaat ccattgcgcc atattttttc
     4081 aatgtttcaa cttgtgtacg aactgtagaa tctttttgtc cgattgctac atagatacag
     4141 atcatatctt gacctttttg gttgatgatc gtatcgatcg caatagaagt tttccctgtt
     4201 ttacggtcac cgatgactaa ttcacgttgt ccgcgtccga ttggcacaag ggcgtcgatc
     4261 gcttttagcc ctgtttgcat tggttcgtta acagatttac gttgcataac acctggtgcc
     4321 atcgcttcta ctggacgagc tttgtcagta acgatttcac ctagtccgtc gattggttga
     4381 cctagcggat tgactacccg accgatcaag gcctctccaa ctggaacttc catgatctta
     4441 cctgttcgtt ttactttatc tccttcacga atggactcga aatcgccaag gataatgata
     4501 cctacatcat ttgactctaa gttttgcgcc attccgtatg agccgtttga aaattcaagc
     4561 aactcaccac tcatcgcgtt ttctaaacca tgtgcacgag cgatcccgtc acctacataa
     4621 gttacggtac cgatttcttc gactgaaagc acgttttgat aattttcaat ttgttcttta
     4681 atcaaggcac taatttcttc tgctttgatg gccattcgat tcacctacct ctttgtacta
     4741 tctgtttaat tgattacgca ttttttctaa ttgcgtgcga atgcttccgt cgattactcg
     4801 atggttggct tcaacaatcg cgccaccaat aatagaagaa tcaacgattt gttttaattc
     4861 aactgattga tagtccattg tttttgcaac attcttttct aattgtattc gttgttcgtc
     4921 tgaaagtggg actgctgttg tgacacttcc caatagtaac ccttgatgtt catcatatcg
     4981 acgttcatat tcttcgatca tgaacaaaag gtcatccatt cggttatact cataaacaac
     5041 ttctaaaaag tttttaacga tcccatcata accgctgaca agcttgtcca tgatttctcg
     5101 tttttcatga ggttctagac gaacatcact taacatattc cctaatccag gtacttgttc
     5161 atagatttga cgtaaactta cgagatcttg atagatttct tctgcttgat ttgaatcgat
     5221 cgccagttca aataaagctt tcccgtaacg tcttcctact gtatatttat ctagtttcat
     5281 ttgaagaacc tagaccttca atgtattgat tgattaatga ttcatgcatt tctggagata
     5341 attctttgtt caagattttt tccgcgattt gaagagagag ttctgcaacg tcgtctttta
     5401 cagagttcag cgctgaatca cgttctacag tgatatcagc ttgggctttg ctttttagac
     5461 gagctgcttc ttcttgagca tccttcaaaa tattttggcg gcttaattct ccactttctt
     5521 tcgcattttt gatgatatca gctgcatcag aacgagaggc taacaattgt tgttcgcgtt
     5581 cttgttccat tttcgctgag ttgatgcgag attgttctgc agaatctaaa tcattggcga
     5641 tcttgtcttc acgttttttc aaaatatcgc tgattggtcc ccaagcaaag tgcttcaaga
     5701 gagccagtag tatcaaaaat gagccgctga caacgatgat attacctaac atcgcattgc
     5761 caacttctgc aattgccaat tgattcagca taggcgagcc ttcctttctt tggagtattt
     5821 ctagtttcca tgtaaaaaag cagtaacaaa cgatgttgtt tctgccttaa tactgattca
     5881 attaaacggc aaaaactaga atcaaggcga taactacccc taagatagga acagcttcaa
     5941 ctagagctac cccgataaac attgttgtac ggaattgacc agacatttca ggttggcgag
     6001 ccattgattc gattgtttta gagataactt gaccgttacc ataaccggct ccgattgctg
     6061 ctcccatgat tgcgattgct gctgcgatat aattcataat tgttttcctc ctaaaataga
     6121 taaatagtag tttgtttttt aaccgagtat tattcttcta cttcaatctt atggctcata
     6181 taaaccattg acaaagtgac aaagacaaat gcttggatgc tcccaataaa caaggagaac
     6241 gcgatccaga ccatctctaa tggaattgct aatggtaatg agaaccagcc taagcttgtc
     6301 atcatttttg caatcaaggt tagcaaaact tcccccgcat aaatattccc ataaagacgt
     6361 aaagcaagtg ttaataaatt tgtaaattct tcaatcagtt tcatcggaaa cataaaacta
     6421 tagggtttca agaacgaatt tacgaaatat cctttgaagc ctaacttttt caccccgaat
     6481 aagtgagtaa gtaagatcat tataaatgcc aaagttaaag taacaaacgg gtctgctgtc
     6541 gggcttttcc aaagtgttgt ttcgttaggt aagacgattt ttgttactaa acctatgttg
     6601 tttgcaacaa aaacaaataa aaacaatgta aatgccaaca aatggaaatt gctcaattct
     6661 tttctaggca gattatctgt tacgattcct cgactaaaat caatgaccca ttctatcgca
     6721 ttttgtttgc ctgttggttt caacttaaga tttcttgtaa aaaagtaagc taagaagaaa
     6781 acaatcaaac aggtcagtaa caccattata caaactgtac catcaaacca aattggtcca
     6841 atgtgaaacg ttagcgagcg ttcatccaat ccatttcacc tcttttcccc gaaattcaca
     6901 taaaatcaat tcatgtgcgt atctggtttt ttccaatcgg tttctaacca cacggtatca
     6961 taccaccatg aaacagaaaa ttcaaaacat tttcatgaaa attagacact tttttatttc
     7021 agtcaaaaac gaagcgaatt tttccccatg cacggtgctc ctgatctaag aaaaaacctg
     7081 tgataatcat gttttatcag attaaatgct aacaaatttt atcattaagt aaaagcaaaa
     7141 gcaccgaata tgcgaaaaag gataatgaaa ttatctagag aaatttttta atgttcgatc
     7201 aatttgtcga tcgatatctt ttcgtttcaa atcttcccgt ttatcatacg attttttccc
     7261 ttttgccaga ccaatcaata ccttggcata gccgttacga atatagactt ttaaaggaac
     7321 aacagtgatt ccagtgtttt tcaattcgtt ttcaagacga acgatttgtt tcttgtgtaa
     7381 taataacttt ctcgtgcgca acggatcatg attaaaaata tttccttgtt cataaggact
     7441 gatatgaaca ttatgcaaga aagcttcccc gttgcggacg cgaataaatc catctttcag
     7501 attgatccgg ctgtttcgta tcgacttgat ctccgttcct tgcaagacca tccctgcttc
     7561 catcgtgtcg atgatcgaat aatcatggcg agctttcttg ttttgtgcaa ttagttttcc
     7621 ctcgcctttt ggcatagctg tttcctccaa ctcattttat tttttctttt tcttcttttt
     7681 gacatctttg taaaatggct ttttcccttt tttcttaccg aacgtttgct tactatcact
     7741 atatcgtttg ttcttcttgc catttttttc tgagcgtttg cctgcttgtt ttccttctcg
     7801 ttttccaaat tttccattgc gtgcttttgg acgttccaac gcagtgacct cttctgcgga
     7861 aacgagttca aaatccactg ctcgttcatc tggatcagct ttgactactt ttacgcgcac
     7921 tttttgtccg attttaaaag tcagaccagt tctttcacct acaagagcta aatgattttc
     7981 aacaaaatgg aagtagtcct gtttcaattc attgatatgg atcaatcctt ctactgtatt
     8041 cggcagttcg ataaacaggc cgaatttcac aacagagctg atgatcccat cgaattcttc
     8101 tcccactttg tccatcatga attctgcttt cttcatggaa tctacttcac gttctgtttc
     8161 tactgcacga cgttccatct tagaactatg atctgcaatt tcaggaagtt ccgcagccca
     8221 cgaactttga ttgctttcac ttttatcttc actgtaagaa cggatcagac gatggacgat
     8281 tagatcggga tagcgtcgaa taggagatgt aaagtgtgtg tagtaatctg ccgctaagcc
     8341 ataatgccca aaattgtctt ctgaatagcg tgcttgttgc atgcttctta gcaacatagt
     8401 attgataact gcagcttctg gtttgtcttc tactccttcg atcacatgct gtaaatcttt
     8461 tggtgtgatc gttcctttcg ttcctcggac cagaatgccc aaagctgaag caaaatcaaa
     8521 gaagcgctgc attttttctt cttttggctg ttcatggatt cggtaaataa acggcagatt
     8581 taatttatta taatgttcag ctaccgtttc attggccgca agcatgaaag attcgatcaa
     8641 gcgttcacca attcctctgc ttcttagctg gatgtcattt gggtgtcctt cagcatcaac
     8701 aaggattttc gcttcgcgat cctcaaaaga aatcgcgcca cgtttgattc gcatcgtttc
     8761 aaggattcga tgcaattcac ccatttcttg gaacataggt acgagctgag catagcgttg
     8821 catcgtttct tcgtcttgat cttctaagat ttgattgaca gctgtgtagg tcatccgttc
     8881 tgttgtttgg atcacacttg ggaaaatatc atgagagact acttgtccat ttggatcaat
     8941 ctccatttcg caactcatgg tcagccttgg aacatgggga ttcagtgaac agataccatt
     9001 cgataaacgt tgcgggatca tcggaatcac tcgatcagtc aaatatacac ttgtccctct
     9061 ttcatatgct tctctgtcca attgactgcc ttgtgtcaca tagtaagaaa catctgcaat
     9121 gtgtactcca agaaaataat taccgttatc taatttttta accgtaactg catcatctag
     9181 atcttttgca tcttctccat caatcgtaac aatgagctga tttcttaaat ctctacgtcc
     9241 agttatatcc ttttcgctga tttgatctgg aacttcatct gcttcagaca atacatcatc
     9301 aggaaatttc gtagggatac cattagccac gacaattgag aggatatcca tgccagggtc
     9361 attcttatga ccaatcactt gcttgattat cccttctaaa ctagtcgcat accctttttc
     9421 agggtaatgg gtcagttcga tcagcacaat acttccgtca actggacgaa ctccctctgc
     9481 tgctgcatat actttaaatt ggttgagctt tctatctttc gggataacaa cgccgtaaag
     9541 atccgtttct tgtatttctt cttctgaata agcaacgaat tcccctgcta actgagtagt
     9601 cgcccggtct cgaatctcga ctactttccc ctctgctccg cgatccatat ctgtatttgc
     9661 tcgcttcatg atatcgattg ctacggtgtc tccatccatt gcaaaaccag tcgcttcttt
     9721 tgggatatag atgtcatctt cttcgggatc aatcgttaca aatccaaatc ctcgttcatt
     9781 tgctcgaaat gttccttcga tcaagattgg ttttaaagga agtttcactt ttcctttttt
     9841 attgaaaact atactctgtt ctctttccat ttgagcaatc gtttgtacta gtaatttaaa
     9901 atcatcgctt ttttggaacc ctaaaccttc agcgatttct tccatagaaa aactgcgttt
     9961 gcgactgctt tccatgaaaa agagaatttt ctcttttatc gtttcttttg tcatatttat
    10021 tcctcattcc aaggtaattt ttctaaaaat tccaagacgt ctttttctaa ttcgcgtctt
    10081 gctgtaccaa ctgtaatcac atgaccactt tcaggatacc atcgaagtgt cactcgctga
    10141 cggctcaact tgcgtgcagt ttcaaatacg ccattagggt cgatcatttc atctttccca
    10201 gcttgagcta aaaatatcgg ctgttggata tctgacaacc tactttctac gattgctgcc
    10261 tgatcttgta tatccattaa ttgttgttca gctaacgagc ggtaagcaac tgctttttca
    10321 ttgatttctt tctctgattt acctgctgtc ttcagcactt gacgtacgta tttttcaaag
    10381 ttttcaggca cgtttgtttt tacaggagac aaaggcgaac agaaaaaacc gccgccaatc
    10441 actgattctt cttctagtgc tcgtacagca aagatgccgc ccattgataa tcctaatacg
    10501 gcaatatcag aaaactgctc agactgtaaa aaatggatgg cttttttagt gtctgcccac
    10561 cagctttctg ctttttcttg caaaatatca tatggctcca ttgttccgtg tcctttaaaa
    10621 agcggcgcat aaactgtata atctgctttt tccaaaaatc gtgcgagcat tcgaacatcg
    10681 tttggacttc ctgagtaagc gtgaagcagc aaaaccgctc gctttccatg ttttgcataa
    10741 aggggttttg gtaattgtct cactcgtctc acctctttcg cctcatttta ccatagtttc
    10801 tttcataact tcttcttaag cgttctcttt gcaataaaaa aagccgcccg ccgttttgcg
    10861 agaggcttat tcaactactt cgaagataaa tatgctagga caaataataa gatcatccag
    10921 atagctccca taactgcagt tgatcgctgc atgacagctt caaagccgcg agctttttgt
    10981 ttgccgaata gttgatccgc accgccggta aatgcactcg ctgcactatt ttgcttgctt
    11041 ggctgcatca taatcgcaat gaccataaca atcgataaaa cgatcacgat tcccaaaatt
    11101 aaattataca tatcgcagcc tccttcccaa aagatatcgt ctctttaact ttatcataga
    11161 taaaagtccg tgacaactct atataaatgg aataacagct gaaagttcaa aactttcagc
    11221 tgttattctt atgactgacg taagacgtct tgatattctg gtttcttttc aaactctttt
    11281 tttgcataag ggcataaagg aatgatcttt ttcccgtctc gtcttgctaa ttctactcca
    11341 gtatacacta gcttttcagc taacttttga ccacgatatg ctggatctac aaatgtatgg
    11401 tcaatgatca tgatgtctgg gcccgcatct gaccatgtca tttctccgat ctcttgttgt
    11461 tcatcattga ataaagcaag acgatttttt tcttctttga tttccatatg ccgcactcct
    11521 caaataactg atattttttt attcctacct tattttagca gtctcctgat ctataaacaa
    11581 ttatttatcc tcgaattact tacaaaaaag aagagaaaat taattcatcg ttattctttg
    11641 ttcaataaat gaaaaaatca ctgtccaata ggttttcgga tctttcatcg atgcttccaa
    11701 gtgttctgct tctggaacaa gcagcagtgc tttatcccct gccgtagcat tttgaatcgt
    11761 gtacgctgct tctgtaggga caaaatgatc tctttcacca tgaatgacca ataaaggcag
    11821 atggttgctg ccaagctgtc tcgtagctga tgcttgcttt aaggagtaac cggcgtattt
    11881 ctgggcataa tgattcgcat atctcatgac cattttttta ggaaagacag tgattttact
    11941 taacatatat cggaattctg cataaacaga aacatatccg ctgtctaaaa taaatccttt
    12001 gaccgctgat ggaagttttt caccacttgc catcatgatc gtagaagctc ccattgatcc
    12061 gccgtgtaaa ataatggacg cattcggctg ttcatctaag atgagttgga tccaggcaat
    12121 taaatctagc cgatctagcc atcccattcc gatgatttct ccttcacttt ctccgtgcgc
    12181 acgtaaatca ggtacaagaa cgttgaatcc tttctctgca tatcttttac ctatatgaga
    12241 catgtctctt ttccctgtgc ttcggtaatc atgtacgcaa atgacccatt tattctgctt
    12301 atttggatgg atgaacatct gaccagctag tttcaacccg tcataacttt tgattgtttt
    12361 gggtgttccc tgctcccaaa agctttcaca ctcttcttct aatttttgtc gttttttttc
    12421 tactgacgtg actgcagcag ggtccatcaa acgatgacca gtgtcttcat accagtcata
    12481 tcctctctgt actcccgctc ttactaattt ctttgcataa aaataagaaa gaaaatggta
    12541 actgatccct ataattatca gaataagaat gaaataaaaa ataaccattg tacacccgcc
    12601 gctttctgcc aaactataat ctaactatta tccaatgtac cgatccatag gattctttat
    12661 tttcggcaca cgtaaattcc atttcttttt cattgtatca tttacttaaa gttctcctca
    12721 atagatttac ttgtaagaga gcatttacat tcgcaataaa agccagtata ctaatttcgt
    12781 gcattataat caagacgctc gtcttggact ggttcggaaa cttcccagaa taaaaaacta
    12841 agtatctcta aagaaaggag attggaaata atgcgaaaag taattattga ttgcgatcca
    12901 gggatcgacg acactttagc tttatcatta gctgtcaaat cccctgatat cgaagtagtt
    12961 gctattaccg tagtttgtgg gaatgtccct gcagatatag gtacgcagaa tgtgctgaaa
    13021 tgcctggaac gctgtgatcg tttagacatt cctgtttatc aaggaatgga aaaacctttg
    13081 aagcaacctt tcatcagtgc acaagacact catggtatgg atggtttagg ggaaacaaat
    13141 ttcccgatga tcttgagaaa acaggcagaa cctctccatg ccgttgattt tttggctgat
    13201 tatttcaaag aaaagacaaa tacatcagtc attgctttgg gtcctttgac caatatcgct
    13261 tttgctttga aagtcaaccc aaatattggg aaacatatgg atcgttttgt ctcgatgggt
    13321 ggaacttaca aaagccatgg taactgttcg ccagttgctg aatacaatta ctggtgtgat
    13381 cctgaagcag cttcttatgt attcgagaat ctcaaacaaa cgatagaaat ggttgggtta
    13441 gatgtgacaa gagaaatcgt cttgacaccg actatcctcg aatactgctg tcaaatgaat
    13501 cctgaagagg gagagtatct aaaagcgatc actcgttttt attttgactt ccactggaaa
    13561 caagaaagaa ttttaggatg tgtcatcaat gatccgttag ctgttgctta ttttatcgag
    13621 ggggccatct gtgaaggttt caaaagcttc actgctgtgg aaactcaagg aataagtcgt
    13681 ggacaaacat tggttgatcg ttatgaattt tggcagaaac ctgctaacag taaaattatg
    13741 acaaaagtag atactcgtct ttttttccga aagtttttaa ccgttctttt aaatgcacaa
    13801 gaagaaacga ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa tgaataaaaa
    13861 caaaatggca actcgcgtac ttacagtgat ggctttgtgt attgggatca attatattgg
    13921 aggaacgatt gctttatggc ttcgtttgcc aatctatctc gattctatcg gaacgatttt
    13981 tgctggtgca ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca gcggtatttt
    14041 aagtggagtg actacagata ttttttctct gtattattcg ccaatccaaa ttctcacagg
    14101 cttgcttgct ggattgattt tgccaagata tctcggcaaa gataaaaaca gcttgaaact
    14161 gttattactc gctttgattt tgtcggtacc tggaacgatc gtttcttcga tcattaccgt
    14221 tcagttattt ggcgggatca cctcctcagg atccagtatg atcgttcagc tattacatgg
    14281 gttaggttta agtcagacaa caagtgtgat cttggtacag gcaggtacag actaccttga
    14341 tcgtttgttg tctatcgtcg ttgtttcatt agtcatcact atgttgccac aacgcttgat
    14401 atattcaaaa agaaaaatag gctgaataaa cagagctgga ttagcaaaat aatgctaatc
    14461 tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa ataattcctt
    14521 gatatttttc aatcatatcc aaataattat cgatttcaac atactcatct acttgatgag
    14581 gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta gctttcgtaa
    14641 attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg ctgaattgtt
    14701 gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt ttgttgtaat
    14761 cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg attgattgca
    14821 gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc ccttggagct
    14881 gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta acattatgga
    14941 ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt tcagcatttg
    15001 ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct ggcatagagc
    15061 tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg tgcgtataca
    15121 tcaaactgta attagttggc tctccgatga tcaaggcatc taagtcatct acatagcctg
    15181 ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg gctaataatt
    15241 tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca atcaccattg
    15301 ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc ccatggattt
    15361 ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg acatccatat
    15421 gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca atcaaattat
    15481 ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc aaaagcttat
    15541 tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg atgatctctt
    15601 gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca tttcttttta
    15661 ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa tgaagaaaga
    15721 gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata cgagaggact
    15781 tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga tgctagttat
    15841 tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta aacatcatat
    15901 acagaagcta gttgcaaaaa aaactctcta cctgctgata tt
//
LOCUS       DACDKL010000053        15900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDKL010000053 DACDKL010000000
VERSION     DACDKL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.053"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..1476
                     /locus_tag="IV329_001270"
     CDS             139..1476
                     /locus_tag="IV329_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8940462.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1524..3653
                     /locus_tag="IV329_001271"
     CDS             1524..3653
                     /locus_tag="IV329_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8940463.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3628..4317
                     /locus_tag="IV329_001272"
     CDS             3628..4317
                     /locus_tag="IV329_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940464.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4725..5411)
                     /locus_tag="IV329_001273"
                     /pseudo
     CDS             complement(4725..5411)
                     /locus_tag="IV329_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5547..5723)
                     /locus_tag="IV329_001274"
     CDS             complement(5547..5723)
                     /locus_tag="IV329_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940465.1"
                     /translation="MIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKR
                     IGGRVKPIQEVRPD"
     gene            complement(5791..6231)
                     /locus_tag="IV329_001275"
     CDS             complement(5791..6231)
                     /locus_tag="IV329_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8940466.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6235..7080)
                     /locus_tag="IV329_001276"
     CDS             complement(6235..7080)
                     /locus_tag="IV329_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8940467.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7229..8647
                     /gene="pepV"
                     /locus_tag="IV329_001277"
     CDS             7229..8647
                     /gene="pepV"
                     /locus_tag="IV329_001277"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8940468.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8704..9651)
                     /locus_tag="IV329_001278"
     CDS             complement(8704..9651)
                     /locus_tag="IV329_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8940469.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9874..10224)
                     /locus_tag="IV329_001279"
     CDS             complement(9874..10224)
                     /locus_tag="IV329_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8940470.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10424..11503
                     /gene="pepA"
                     /locus_tag="IV329_001280"
     CDS             10424..11503
                     /gene="pepA"
                     /locus_tag="IV329_001280"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8940471.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11647..11967
                     /locus_tag="IV329_001281"
     CDS             11647..11967
                     /locus_tag="IV329_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8940472.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11989..12453
                     /locus_tag="IV329_001282"
     CDS             11989..12453
                     /locus_tag="IV329_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8940473.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12658..13263
                     /locus_tag="IV329_001283"
     CDS             12658..13263
                     /locus_tag="IV329_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8940474.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13302..14591)
                     /locus_tag="IV329_001284"
     CDS             complement(13302..14591)
                     /locus_tag="IV329_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8940475.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15019..15498
                     /gene="rlmH"
                     /locus_tag="IV329_001285"
     CDS             15019..15498
                     /gene="rlmH"
                     /locus_tag="IV329_001285"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8940476.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15701..15900
                     /locus_tag="IV329_001286"
                     /pseudo
     CDS             15701..15900
                     /locus_tag="IV329_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5009 a         2933 c         3246 g         4712 t
ORIGIN      
        1 gcataattaa ggttttttaa ttgaaaactg aacatttatg gtcacccttt aatgaaagcg
       61 ctaactacat aagttagaat gagtttgact gatatattag tttgcttatc agaaaaacaa
      121 aatggacgga ggaataacat gagtgatatt attattggta ttttgttagt tatctcattc
      181 tttttcatgg tctggtactg tgtaaaaggc tacaacttga tggtcggctt tgccatcatg
      241 gctactgtat ggatgggatt agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg
      301 gaagggcaag gagtaattga tgtgttgacg catatctata cgacggggcc agctgagtac
      361 gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat
      421 agcggaatcg cagctaccct gattcgaaag gtagttgaac ttggagggga caagccaaga
      481 atcacgatga gcttactatg tattgtaacc gctgttatat tcatgtcgat gacaggaatc
      541 ggaccagtga tttctattgc agttattgtg ttgccaatct tgatgtcatt aggtatttca
      601 gtaccggttg cactgttctc atttatgggc tcgattatgg ctggaatctt tgctaatatc
      661 gtcaatttca aacaatatca aacgatttat gctggtttca atccagcggc tgaaagctat
      721 acttataatg actatttcca aattggtatg attgggatga ttgtaagctt agttgtggta
      781 ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct atgcaatggc agcaaacgta
      841 ccagctgaag gtggcgacgc accaatgatc tcatggttag ccgtattatt accagtttta
      901 ggggtcgttc ttttagatct tccaatcatc ttaggtttta ttctagcagg tatctgggca
      961 ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa
     1021 ttatttacag atggcgcagt agatgttgcg ccaatggttg gtttcttgat gaccttagcg
     1081 atgttcaata attcagctgc gtatgcttct ccttattttt cagctatttt tggagattgg
     1141 ataccgcaat caccattagt cttggcaatc gtatttgcta tattgacacc actaggattt
     1201 ttccgcggtc caatgaatct agtcggttca ggctcagcta ttttagctgt cgtattagct
     1261 gttaatccaa cgatgtcgcc agcctttttg ttcccgttat ttgcaatcac aacgattgct
     1321 cctcagcatt tagatattac acaatcttgg gttgcttggg gattaggtta tacaaaagta
     1381 acttctcgag aatatatgaa gaaatccatc ccaactggtt ggatcatcgg agcgatctta
     1441 tgcctgatca cgttcttatt gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga
     1501 ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg
     1561 gtacacatac gaaagcagtc gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt
     1621 cagtgaaaac aacccacgat gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc
     1681 aaaattgttt gctggaaaat aatatcagtc cagaagatgt tgtgtttgtt gcacattcta
     1741 ccacacaagc gactaatgca ctgattgaag gagacgtagc aaaagtcggc gtaatcggga
     1801 tggcaaaagg tggattagaa ggttttttag caaaacgaca aactagattg aacgatatcg
     1861 atttagggaa taagaaaaaa atagagattg tcaatgcatt cttacctgtc aaacacttaa
     1921 atgtagaccg tgtttcagaa acgatttctt ctttagaaag agaaagagcg gaggtcttgg
     1981 tttcttctat ggcatttgga gtagataatg gggaaccaga acgagttgtt tatgaagcag
     2041 catcggtaaa atcgatccct acaacgatgg cttctgacat cacaaaatta tatggattga
     2101 caagaagaac tcggacagca gcaatcaatg catctatttt accaaaaatg ctagatacgg
     2161 caacctctac agaagattcc gtacgagaag caggggtcaa tgtttctttg atgattatgc
     2221 gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc
     2281 tttccggtcc agcagcatca gtcatgggct ctttgatgta cttgagagcg tctaatggtg
     2341 tttattttga agtaggcgga acaacaacca atatcggggt cattaaaaat ggacgtccag
     2401 caatcgatta ttcaattgta ggtggccacc caacatatat ctcttcatta gatgttcggg
     2461 ttctaggtgt ggcaggcggt tcgatggtac gggctaatca gtcaggaatc atcgatgtcg
     2521 gtccacgttc tgcacatatt gccggtctag attatgcagt atttacggag acagaaaaaa
     2581 tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg agaccctgct gattacgtca
     2641 aggtagtgat ggaagacgga gaagaagtga ctattacaaa tacatgtgca gccaatgtcc
     2701 ttggattagt gcaagaagaa catttttctt atggaaatgt cccatctgca cgaaaagcca
     2761 tccaagcatt agctgattat tgtcatacga cggttgaaga catcgcagaa cagatcatgg
     2821 aaaaatccta tgctaagatt gaaccggtga ttttagaatt ggcagacaag tatcacttgg
     2881 agaaagatca gatttctctt gtaggtgtag ggggcggcgc tgcctcattg attacttact
     2941 ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa cgccgaagtg atttcttcta
     3001 ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg tatcatccct tcgccaagta
     3061 aagaagatat acgatcgttg aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta
     3121 caccagaatc gatcgaagtc cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta
     3181 tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac caaggagatc acaacagaag
     3241 aagcactaga gcttgctgca gaggatatgc gattaaataa aaatgaagtt tgcttattgg
     3301 aaaatacacc tttcttttat gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc
     3361 ttcggatcat cgatcaaaaa ggatttatca aggtccaacg tggacatgct tcatgtatga
     3421 aaacaactgc agccaattat atgacagctg tcgaacaact ttgggaagat atggcagttt
     3481 atcaaactga attgattgcc cgaccagaat tttatctctg cttaggagca agagtctctg
     3541 attttacagc gacggatctg gaacaactac agcttttgat ggatctggaa gtatctacta
     3601 tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat caaacaaaca taagttagaa
     3661 gagttactaa acgaattaca acagatcccc gaagaaattt ggggattcta ccagtttcgg
     3721 cgagatttat tttggaaaaa gattcctttg tccaagcaga aaattcttat acaacaatca
     3781 atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta
     3841 ggagaaattt gtgaacaaat ggctgttcca atcgtttcct gtgaatcaga gcaaataaac
     3901 gaacgaatca cttttgcaac ttatgcagaa gatgaaggga ttcgattgat gacggagccg
     3961 ttagaaaaat taaaatgttc aggactagct tcgatctcta aagaaacagc gcaagctttg
     4021 attattggac atgaactatt tcaccacatc gaagcttctg ttaagggaat ttatacgcaa
     4081 agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac atcaatcaaa cattcgtgca
     4141 ttgagcgaga tagcagcgat gagtttttca aaagagatga atcaaagcga tttttcgcct
     4201 tatgtattag aggcagtctt actttggcca tataatgaaa ttcgtagcca agggatctta
     4261 gaagaaataa aaaaaataga aaaaagatgt acggagtatg atttagatta caaataaaga
     4321 gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa taaacggtgt tctcctgtca
     4381 actcctcggt cacaagtcaa aatattcaac aaacatgaga agctgttttt tgaaattgtt
     4441 ccttgtgact cggagatgaa cggcaggctc acgacctctt ggaaaacggt gttcaaaagc
     4501 ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctatt tttacttggc
     4561 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat
     4621 cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat atgaggaact attttgccgt
     4681 ttctttctct aatactcagg tcaaaacgct gattccaaaa cctcttattt gatgggtctg
     4741 tcctgaatat cttcaaaata atcggggtaa ttatgaatca cgtattcttg ctcctcgata
     4801 atcatatgta aatgcatcgt gcaaagaaat ccgacttcat caaagttatg atcaatcatt
     4861 gattgaagca ttgtctgatg ttcatctaat actcgacccc agtctaattc tgaaatcgtc
     4921 aaacgtaacc atctgaaacg gttcaagtgc gtactataac tttctagcca atcccaaatt
     4981 tctcgttttc cagcaatttc ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa
     5041 gcttttttat tttgagcgat tactgcctga tcggtttgtt ctagtatttt ttctagttta
     5101 gcttgatctt acttggtcat tttcgcactg cattcttgca ggatcggttt ttccaacttt
     5161 tcccgcgtat aacgggctaa tgaagcagat cggatatcga tttttgaaac ataagtgccg
     5221 gactgtggaa tggtttttac aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc
     5281 ggtgtacgcc cgatagctag ttgttcttct aacactttat cggagatttt acttccaggt
     5341 gctaactcgg aataaatgat ttgtttacgt atagattcat aagcttgctg ttgcaaatta
     5401 ttgataacca tttaaccttc ctttctatgc agatataatc tattataaca taaatcgctt
     5461 acaaatcgtg tgggcataaa aacacagtct gcaaaaaaag ccctgatagg catgcctatt
     5521 agagcctcga aaagaaaaat attgaatcaa tctggtcgta cctcttggat cggtttgaca
     5581 cgaccgccga ttcttttagc aaaagcttcg gattcttttt tgtctttgaa aatgagcaga
     5641 ttgtctaagt ttttttcccg agtgcgacag ccagttttat cgatgtaacc gtgcattact
     5701 ttaacgacat acatggggat cacctgcttt tttcttttca ttatacgaac tactcgacac
     5761 aatacaagaa aaatgcttag acggtcttta ttaagaattt acatcatcca aagaagcgta
     5821 cgcttggaat aactgatcta actccggatt gactttgctc agcttcacta atcgttgcga
     5881 atcagaaagt aaaaaacagt gtttactact gccagtagtt tttaattttt tgtcttcagt
     5941 tcccacaatt tcgtagctaa aatccaaccg tgtccccgta tacttttgga tagttggatg
     6001 gatgctcact ttttctccgt aatggatcat ttctttatag ctgcaattta cttccagaac
     6061 ggggatgatg atcccagatt cttcgatttt tttataggga aaccctagat gtaccaataa
     6121 ggctacccga gcctcttcaa accagcggat ataattgctg tgatgaataa tccccatttg
     6181 atctgtttca taataaaaag gttctcgcac ataacctgta aatttctcca tgactcatgc
     6241 ctcgttttcc attgattttt tacaaaaata attcatccat ttcggaagtg cttcgctgat
     6301 ttgtgtcggc aaaacaatat attgttcttt tgccagctgg tctccaatga aactatgaag
     6361 ataaatagct gctgcaatcg tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat
     6421 catcccagct aaagtgtcac ccatgccgcc cgttgccata gcagcagaac ctaaaggatt
     6481 ggtgtagaag tgctggtgac cataaatttt tgtctcatga cttttcaaaa cgagcacagc
     6541 accgatttcc tgttgcttct ttttgttttc tttatcccgt tgttcttgta tcggtagaga
     6601 actcaaccgt tgccactcca tttgatgagg tgtaagtaca gtttgttcgg gatagggtag
     6661 caaaagatga ttagcagcaa gtaaacttaa agcagagcca tcaatcacca gccattgttc
     6721 ttttgattgg ttttctagta cttgtttgaa acgttccaaa ctcttagaag aagtacccaa
     6781 tccgggacca atcaagagta catctgcttg ctttattacg gaaagaaact ggtcagtatc
     6841 cgtccaaact acaatcattg cttctggcag atgactgtga agagcactcc gatttttttc
     6901 atcagttact acagtcgtga gacccgctcc gctgtgtacg gcagctaaag cactcataat
     6961 gattgctccg ccatattgtt cattgccgcc aatcaaaacg atacgtccaa atgttccttt
     7021 atgtgaaatt tttggacgag gtgtgatgac tcttttcact agttgttcgt ttatcagcat
     7081 ctaaaaacct ccttttactc attatagcgt atattttttt tagagaaaaa cagataagaa
     7141 aaggaattgt cagatttttt gaactatttt tttctgtaat ttgtggtatc atgaacacga
     7201 agacttaaat aagtggagga ttaactttat gacaattgat tggcaaaaag aagtagaagc
     7261 acgtaaagaa gacttgcttg aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga
     7321 tgactcaaaa gtaacaccag atgcaccatt tggacctgga cctcgcgacg cattgaaaca
     7381 tatgttagct tatggcgaac gtgatggatt taaagtaaaa aatgtcgata actatgcagg
     7441 gcatattgat cttggcgaag gagacgaaac gctagggata tttggacaca tggatgtcgt
     7501 tcctgcagga gatggctggg acacagatcc ttatgaacca gtaatcaagg atggaaagat
     7561 ctttgcacgc ggatcaagcg atgacaaagg tccaagtatg gcagcttact atgcgatgaa
     7621 gatcattaaa gaacttgact tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga
     7681 tgaagaaagc ggctggggcg atatggctta ttatttcgaa catgaagaag aaccagattt
     7741 tggtttctca ccagatgctg aattcccaat catcaatggt gaaaaaggaa atgtttcttt
     7801 agctttacgc ttcaaaggcg acaatgcagg agattatgtc ttgaaatctt ttgtttccgg
     7861 acttcgcgaa aatatggtgc caggaacagc aactgcagca cttcaagtac caagtgcaga
     7921 tgcagctatc gcgatggaag aagcatttta ccaattcatc gaagcaaatc cagtaagcgg
     7981 aacgattgaa gcggacaata cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg
     8041 tgcaagccca caatctggta tcaatgcagg ttctttcttg gcattgttct tagataacta
     8101 tgaattccta ggatctgcta aacaatttat ccatgtagca gctgcttatg tccatgaaga
     8161 tttttatgga gaaaaactag gagtcgctta tgaagatgaa aaaatgggca aattgacaat
     8221 gaatgctgga ctatttgctt ttgaagaaaa tggtacagag gaagcgaact tcatcaatat
     8281 gaatttccgt tttccaaaag gtgtaacagt agacggattg caaagtgaca tcgaacaaac
     8341 agtggggcaa gaaggcgcaa cagttactcg aggtgctcgc gtaatggaac cacattacgt
     8401 accaatggat gatccattgg tggctacatt attgcaagtc tatgaagacc acactggaga
     8461 aaaaggctac gaacaaatca tcggtggcgg aacttacgga cgtttgttga aacgtggcgt
     8521 tgcttatgga gcgatgttcc caggatatac tgatacaatg catcaagcaa acgaatttat
     8581 gagtcttgat gacttgttca gagcaacagc aatctatgca gatgccatct atcgtttggc
     8641 gaaataatta attaaaaaga ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt
     8701 tttttacttt ctactgtggt attcatgata aacatgatat agattcaatt catattcttt
     8761 cttagcgttc gtgacgactg tatccaaaat caaccctgtt actaatgaaa gaagtgctaa
     8821 agtcatcaat ccagtactta aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat
     8881 atattcgcga atcacaggaa tccccgtgac caatccaaat aaaaagaaga agatggtcca
     8941 aatcccaaag aaaagcaaag gcttataatc tttaaacatt ttaataatca tcatgatcac
     9001 tttgaatccg tcagagaatg tattcaattt tgattcgctg ccttcaggac gatcacgata
     9061 atcgattggg atttcaacga acttgaattt tttatctaaa gcatggatcg tcaattccgt
     9121 ttcgatttga aaaccagaac tcataatagg aaaacttttg acaaagatac gattgaatcc
     9181 tcgatagccg gtcatgacat caagaatttt tgttttatac attctagtaa ttgtatttct
     9241 aacaaggtta tttccaaaat cgtggaaagg tctcttattc tcatcaaagt aggttccatt
     9301 tgataggcga tcaccaatga ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc
     9361 atgtacagct tctgcaggat aagtatcatc gccatctacc attaaataat agtcggcatc
     9421 gatatcaaag aacatttggc ggatcacatt tccttttcct tgacgaggtt ctttttttac
     9481 gattgctcca ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat tatcatagac
     9541 ataaatatct gcttctggta attctcgttt aaagtctgca atgactgttg aaattgttgc
     9601 ttcctcattg taacaaggaa tcagaacagc aattttttct tgttgttcca tttttcactc
     9661 tccttaacta tagaaaacaa aagctaaatt aaaaatacct cataatcaaa ggaaatgcta
     9721 gtttttcccg ctaaatatca tttatttaaa aagaaaatac tggaatatat gtttggaaag
     9781 ctctatctaa aaaaccaccc actacaaaag aaggacatga tacctctctt ttgaatagtg
     9841 ggtgcgtatt cgttctctca cttttttgac gtattaatct gttgcacctt tttttcttga
     9901 aatttctaat acggttcctg tatgagcatc tgctaaaaat tcataataaa cagtttcgcc
     9961 atcttctatt cgagaaagac ctccccgata agcactcgac cgaacggcga atttcctaat
    10021 aggttccttt tcaaaagaga tccaagaacc ttcgattgca ccttctttta gaaaagatga
    10081 cttgatattt tcaagtactt ggtctgctga caatactttt ttgcgatgat accaaatcgt
    10141 tgaagctacc ccgctcaata atcctagacc gattccaact gctaacccgc ctttaaaata
    10201 gcttagttcg ctttcttcat acatgtggac atacctcctt cttctcactt gttactcctt
    10261 gtgcttattt taccacactc tcatattttc tttgaaataa ataaccgcta ccagaaaaaa
    10321 taagctataa taaatacgat cggtctgcag aaaaaatcat attttatccg cagatcaata
    10381 taagtctgag aggagtttag tctgaaattt agacgaatag catatggaag aaaaaacatt
    10441 tcaacgaatc aaagagttaa cagaattaca aggaacaagc ggttttgaac aagatgttcg
    10501 cgcttatatg gaaagtcata tggaaccgct ggtagatgag ttacagttag atggtttggg
    10561 cggcattttt ggtctgcgcc atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc
    10621 acatatggat gaagtcggat tcatgctgac acaaatccaa gaaaacgggc tttttaaagt
    10681 cgtacctcta ggcggctgga atccttacgt tgtttctgct cagcgtttta cacttaaaac
    10741 ctctactggt aagaattatc catgtatttc ttcttccgtt cccccacatt tattgcgagg
    10801 aacaagcgga caaaaatcag tagaagtaac cgatattttg ttcgatgctg ggtttgaatc
    10861 aagagaagag gcaatgagtt acggtgtcct tccaggagac acaatcgttc cttacgctga
    10921 aacgatcaaa acagccaacg gaaagaatat tatcagtaaa tcatgggaca accgctatgg
    10981 ctgtacaatg gtattagaag ctttagaagc gttgcaaaat gaaacattag gtcatacatt
    11041 gattgcaggc gcgaatgtcc aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa
    11101 taaattcaag ccagatctgt tctttgcagt agactgctct gcagcagatg atacagtgac
    11161 gaaaaatggc acattcggac atttaggaga aggtacattg atgcgtatcc aagatccagg
    11221 tttgatcatg cttcctcgtt tgcgcgagta cttgctggat atcgctgaaa caaacaatat
    11281 cccttatcaa tactttgttt caaaaggcgg tacagatgca ggtgctgctc atacacaaaa
    11341 cgaaggaatc ccaagtacag tgattggcgt agtgggacgt tatatccata cgcatcaaac
    11401 gatgttcagt attcgcgatt ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg
    11461 tttggataaa tcaacagtaa atacgatcgt atacggaaaa taatgcgata gtgaaactat
    11521 aaaatctggc aaaagattgc tttttgccag atttttttta taaaaagagg atcaatgttt
    11581 gttagttcga aagaaaggtg tataatttaa ttgaaaaata cataaacaga aagtagagga
    11641 caaagaatga ttatcccaaa tagtttagaa gaattagcag gctacgtaga aactggaaag
    11701 agtgtatttt tctttactgc cggctggtgc ggagactgcc gttttatcaa accgcaaatg
    11761 cctgaaattg aaaatgactt ttctgattgg caatttattg aagtagaccg tgacaagtac
    11821 atagatgtag ccgctgaatg gaacattttt ggtatcccaa gttttgtcgt gatccaagac
    11881 ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg
    11941 ttcctagaaa gtgtaacaaa aggctgacat ctgttaggag gggtcaagat gaatcaagaa
    12001 tataagaata ttttagtagg tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa
    12061 gcggtagaag tggctcgccg taataatggg aaagtatatg tagcaaacgt aatcgatcag
    12121 caattttata actttatggg ctatgcacct attgatcaaa atctagtcga tcagcaaact
    12181 gaaacagcca aggaaatgat cgaagaatgt aaagaatatg ggaaatctgt tgattacaca
    12241 aatatagaag gaattattgc atatggttct gttcgtgaat ccatggcagt caaattgcca
    12301 aaaaaatatg agatcgatct aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc
    12361 tttatgacag gaagtgtagc cagctacgtg atacgtcatt caccttgtga tgtcttgatc
    12421 gtttctgacg aagaagaaca gccaagtaat taagcaagtg gaacttgtac gaccttttta
    12481 atcaattaac taaaacagta aagggactgt gacaaaagtc ttgtcacagt cccttgttac
    12541 gaataaatgg tgttccagaa gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc
    12601 agtgaatgca attttttgtt aagataaatc tcagcaaagg ataatggagg aaagaacatg
    12661 atttttgctt ataacaaagc acacgtcgga gacaccttga tggttattat agccgacgat
    12721 aaaggaacag aaaacactgt tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa
    12781 ggtcaagttg ttgcttggaa ctttttccat atctccgatc atctaacaat cgaagggaac
    12841 ggccaagtaa gcttaacaga tgaggaatta gcgaaattga atcgattgat caaagaagca
    12901 ggattcgaag aacagcttga agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa
    12961 acatgcgtcc cacatcctga ttctgaccat ttatcgatta ctgagacaga agtagacaat
    13021 ggtacagtat tgcaaatcgt ttgcggtgca cccaatatcg aagctggaca aaaagttgtc
    13081 gttgcaaaac caggagctat gatgccagat ggaatgatga tctggccagg aactttgcga
    13141 ggggtagata gctatggaat gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca
    13201 gcaaaaaaag gaatcttagt attgcctgag gatgccgtag tcggagaagc atttccaaaa
    13261 taaagaggtt gtgacaagtc tttgtcacaa cctctttttt attaattttc actattttga
    13321 ttttgtttta atgccgattg atcgatcgtc aattcaacag tagttgtttt ttctttcgag
    13381 ccttcatagt aagtgatttt gacagaatct cccacttttt tcttatacaa agcagattga
    13441 agttctacac cagaagaaac ttctttgtca tcgatcttag taatgacgtc gtattgtttc
    13501 aatccagctt tttcagccgg tgtagctgtt tggacatttg tcacgatgac accgttagtt
    13561 acagatgaag gaatcttcaa gatttgttct tgttgctgag tagaaacggc agataagtcg
    13621 accattgtga tacctagagc tggacgtgtc acttttccgt ctttttccag ttgattgatg
    13681 atattcacta catcgttact tggaatagca aatcccattc cttctacgct tacattagaa
    13741 gtagattcag aagtacttgc gatcttactt gagttgatac cgataacttg tccttcgata
    13801 ttaactaaag gaccaccaga atttccaggg ttgattgctg catctgtttg gatcgcattg
    13861 atattgactg tttcgttcga ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg
    13921 attcctgaag tgacagaatt tgcatattca gatcctagtg gggaaccaat cgcaatcgca
    13981 ggctcaccaa ccttcagagc acttgagtca ccaaaagagg caactgtttc gactttgtct
    14041 gaattgatct tcaacaccgc taaatctgaa taagcatctg ttccaactaa ttcagctttt
    14101 accttcgtac cgtctttcat caagacttca agaccttgct ggccatctac tacgtgatta
    14161 ttcgtgacta cataagcagt attcccgtct tttttgtaga tcacgccgct tccttcacta
    14221 taagcttcca aattactatc atctgagctg ctactttctt cttgctgccc gaatagtccg
    14281 ccaaaaccgc ttgattggtt ttgactttgt aagttgataa cagaaacgac agcgtcttga
    14341 actttatcta ccgctttggt gatatcagaa tctacattga ctttgacatt ttccacaact
    14401 gtttcaccgg cagaattctg attgccgctt gtagcagtag aactattgtt gccagtgccc
    14461 atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa cgatccctcc aacaagcccc
    14521 agacctaact tacgccagag gctattattt ttactttttt tcatctttgg tgtcacattt
    14581 tttctatcca tgtgttcctc ctccaattac attcatttat ctactgttag tatagtctta
    14641 tttgttgact ttagtctact ttgagactgt gaacttttta tgaaaaactt taggaaaaat
    14701 cattgttttc agatgaaaat atttgatttt ctttttccac agatactgtg taaaactacg
    14761 cggataggat tggaaaaata agggataaaa catatccaca gcctgtgtat aaagtggata
    14821 aattcttata aaacgtatgt ttgcctaatg aatgtgatga aataaggtaa aataacactg
    14881 ttttctgtgg ataaacctgt ggatgttgtg cataactgtt ggggaaatct ttttaacaaa
    14941 aatagccaaa ataaacatat agacgaaaaa attgattata ataaacaaag tgagcatgaa
    15001 gaccaaagga gagaaaacat gaatatcaaa attatctcag taggaaaatt aaaagaaaaa
    15061 tacctgatcc aaggtatcaa tgagtatgtc aaacggctga atgcctatgc aaaaatcgaa
    15121 ttgatcgaag ttcctgacga aaaagccccg gagaatttaa gcgaagcgca aatgagacaa
    15181 gtaaaagaaa aagaagggga acggatctta gcgaaaatca aagaacaaga atacgtctat
    15241 gccttagcaa ttgaagggaa aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa
    15301 ttaggtatcc aaggaaaaag ccaccttgtt tttgttatcg gcggctcttt aggactcagc
    15361 gaagccgtca tgaaaagaag caatgctcag atctcttttg gaaaaatgac gtatccacat
    15421 caattgatgc gtctgatcct tgtcgagcaa atctaccgtg ccttccggat caatgcgggg
    15481 gctccatacc acaaataatg atatttttta agagaaaaaa ttatccctat tttaaataat
    15541 agcaataggt agtggtaggg agaaatttat aaataacttt tttatttgct ttggtagcca
    15601 agtaagttgc aaaagaaaaa tagatactgt taaaaagata atgtagacat agaaacaaat
    15661 acgtaaaatt ttagttagaa taatggcaag gagaaaaaac atgatagatg aaaaaaagtg
    15721 tttaaaaaat ctattagagg atatggatga tattgaagaa aactatctaa atggactggg
    15781 aaaacaaata tatttcagat acttaagcta tctaaaaatg aaataagaca tagtaatttt
    15841 ttagcttttt tgtttgctcc aaatgaaacc cacaatttat ctgatgagtt ttttaaaatg
//
LOCUS       DACDKL010000054        15619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDKL010000054 DACDKL010000000
VERSION     DACDKL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.054"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(481..3186)
                     /locus_tag="IV329_001287"
     CDS             complement(481..3186)
                     /locus_tag="IV329_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8940477.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3492..4439)
                     /locus_tag="IV329_001288"
     CDS             complement(3492..4439)
                     /locus_tag="IV329_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8940478.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4629..4898
                     /gene="rpsN"
                     /locus_tag="IV329_001289"
     CDS             4629..4898
                     /gene="rpsN"
                     /locus_tag="IV329_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8940479.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5074..5436)
                     /locus_tag="IV329_001290"
     CDS             complement(5074..5436)
                     /locus_tag="IV329_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8940480.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5447..6568)
                     /locus_tag="IV329_001291"
     CDS             complement(5447..6568)
                     /locus_tag="IV329_001291"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8940481.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6584..6934)
                     /locus_tag="IV329_001292"
     CDS             complement(6584..6934)
                     /locus_tag="IV329_001292"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8940482.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7064..8575)
                     /locus_tag="IV329_001293"
     CDS             complement(7064..8575)
                     /locus_tag="IV329_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8940483.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8897..10249)
                     /locus_tag="IV329_001294"
     CDS             complement(8897..10249)
                     /locus_tag="IV329_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8940484.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10402..11478)
                     /locus_tag="IV329_001295"
     CDS             complement(10402..11478)
                     /locus_tag="IV329_001295"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8940485.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11674..13419
                     /locus_tag="IV329_001296"
     CDS             11674..13419
                     /locus_tag="IV329_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8940486.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13699..14136)
                     /locus_tag="IV329_001297"
     CDS             complement(13699..14136)
                     /locus_tag="IV329_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8940487.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14120..15493)
                     /locus_tag="IV329_001298"
     CDS             complement(14120..15493)
                     /locus_tag="IV329_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940488.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
BASE COUNT         4357 a         3309 c         2884 g         5069 t
ORIGIN      
        1 ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct
       61 aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag
      121 aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt
      181 tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg
      241 gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca taagaagctg
      301 tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac
      361 acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac
      421 tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt
      481 ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat
      541 gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca
      601 gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac
      661 cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg agacttggat
      721 cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt
      781 accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat
      841 caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat
      901 attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg
      961 agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc
     1021 tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa
     1081 cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac
     1141 aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc
     1201 gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc
     1261 tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac
     1321 ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg
     1381 ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc
     1441 attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt
     1501 cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt
     1561 ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa
     1621 gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg
     1681 gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc
     1741 attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc
     1801 ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg
     1861 ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg
     1921 caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc
     1981 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
     2041 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
     2101 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
     2161 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
     2221 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
     2281 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
     2341 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
     2401 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
     2461 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
     2521 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
     2581 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
     2641 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
     2701 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
     2761 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
     2821 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
     2881 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
     2941 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
     3001 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
     3061 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
     3121 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
     3181 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
     3241 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
     3301 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
     3361 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
     3421 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
     3481 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
     3541 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
     3601 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
     3661 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
     3721 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
     3781 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
     3841 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
     3901 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
     3961 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
     4021 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
     4081 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
     4141 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
     4201 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
     4261 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
     4321 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
     4381 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
     4441 gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg
     4501 tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt
     4561 aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga
     4621 atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg
     4681 aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta
     4741 aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc
     4801 ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata
     4861 aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa
     4921 aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta
     4981 gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt
     5041 atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc
     5101 gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg
     5161 gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac
     5221 ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg
     5281 tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag
     5341 attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga
     5401 aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt
     5461 ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat
     5521 ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg
     5581 ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa
     5641 tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc
     5701 catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt
     5761 ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca
     5821 gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc
     5881 cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg
     5941 ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta
     6001 taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat
     6061 gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt
     6121 ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa
     6181 tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt
     6241 cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag
     6301 gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt
     6361 catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt
     6421 caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg
     6481 gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt
     6541 tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa
     6601 tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag
     6661 gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg
     6721 taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc
     6781 cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag
     6841 aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa
     6901 gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta
     6961 gaacactctc attctaccaa attataacca ttttgccaca aataagtcca caaataacaa
     7021 aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac
     7081 ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat
     7141 tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat
     7201 tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca
     7261 atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt
     7321 ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt
     7381 gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac
     7441 ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt
     7501 tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag
     7561 acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat
     7621 cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag
     7681 caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca
     7741 ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta
     7801 atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag
     7861 gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga
     7921 catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg
     7981 gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa
     8041 gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc
     8101 ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca
     8161 atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac
     8221 gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac
     8281 gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac
     8341 gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca
     8401 ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt
     8461 ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag
     8521 cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa
     8581 tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg
     8641 gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc
     8701 ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc
     8761 gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac
     8821 cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct
     8881 gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca aataagccca
     8941 tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt ttgatagcat
     9001 caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt
     9061 tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct
     9121 caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca
     9181 gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta
     9241 ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat
     9301 ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc
     9361 gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg
     9421 ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg
     9481 tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg
     9541 ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca
     9601 caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc
     9661 cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt
     9721 ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca
     9781 aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat
     9841 tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta
     9901 gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt
     9961 tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa
    10021 ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt
    10081 ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata
    10141 aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag
    10201 agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc
    10261 ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt
    10321 ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc
    10381 cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta
    10441 tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt
    10501 ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt
    10561 caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc
    10621 taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac
    10681 ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa
    10741 tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac
    10801 agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg
    10861 ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac
    10921 actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg
    10981 atcaaatact tttttaggat tttctttcca ttgattctta agtactggta cataaggaac
    11041 ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc
    11101 actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat
    11161 cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt
    11221 gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc
    11281 ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac
    11341 ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc
    11401 atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata
    11461 tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc
    11521 tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc
    11581 ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat
    11641 aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc
    11701 ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa
    11761 aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata
    11821 ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa
    11881 acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca
    11941 gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct
    12001 gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag
    12061 atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg
    12121 atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa
    12181 gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa
    12241 caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct
    12301 ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta
    12361 atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa
    12421 caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt
    12481 ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag
    12541 acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt
    12601 cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt
    12661 ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg
    12721 ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa
    12781 ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat
    12841 gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt
    12901 catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca
    12961 ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct
    13021 tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga
    13081 ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta
    13141 ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa
    13201 ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca
    13261 ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt
    13321 tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc
    13381 gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga
    13441 atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa
    13501 ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag
    13561 ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt
    13621 ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc
    13681 ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg
    13741 atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc
    13801 actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga
    13861 gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt
    13921 gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc
    13981 gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac
    14041 actggtcacg ataaccacgt caagaccata ggattctcct aattttatga catcttcttt
    14101 tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat
    14161 gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa
    14221 gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga
    14281 ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga
    14341 tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag
    14401 ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac
    14461 taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac
    14521 tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc
    14581 cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt
    14641 tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta
    14701 gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct
    14761 ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat
    14821 caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga
    14881 atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat
    14941 ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat
    15001 aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga
    15061 gaaatagcca gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg
    15121 tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta
    15181 gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg
    15241 aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc
    15301 gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca
    15361 aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa
    15421 taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga
    15481 gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt
    15541 tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag
    15601 aaagaagcat aagcttctt
//
LOCUS       DACDKL010000055        15362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDKL010000055 DACDKL010000000
VERSION     DACDKL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.055"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1235
                     /locus_tag="IV329_001299"
     CDS             <1..1235
                     /locus_tag="IV329_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8940489.1"
                     /translation="DKKVFKATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEI
                     HENEDYSMINVATSGGGKIRDYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKD
                     GAEFSSLFPFICRLDNDNEVDDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRL
                     TFMTKRMNRPMEDTRFAVASYDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLG
                     KYDKDVYFTQHTFIHESALRLQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEM
                     SNKYYIKKIAMDMYRAKILKPALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFF
                     GDDAIMRWYCMNVYEEHISNGNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVT
                     VTNSSVENTGTGFTPLVF"
     gene            1255..2433
                     /locus_tag="IV329_001300"
     CDS             1255..2433
                     /locus_tag="IV329_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8940490.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            2396..3082
                     /locus_tag="IV329_001301"
     CDS             2396..3082
                     /locus_tag="IV329_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8940491.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            3082..4242
                     /locus_tag="IV329_001302"
     CDS             3082..4242
                     /locus_tag="IV329_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8940492.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            4252..5127
                     /locus_tag="IV329_001303"
     CDS             4252..5127
                     /locus_tag="IV329_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940493.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            5124..5435
                     /locus_tag="IV329_001304"
     CDS             5124..5435
                     /locus_tag="IV329_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940494.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            5425..5778
                     /locus_tag="IV329_001305"
     CDS             5425..5778
                     /locus_tag="IV329_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8940495.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            5768..6169
                     /locus_tag="IV329_001306"
     CDS             5768..6169
                     /locus_tag="IV329_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940496.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            6162..6566
                     /locus_tag="IV329_001307"
     CDS             6162..6566
                     /locus_tag="IV329_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940497.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            6578..7186
                     /locus_tag="IV329_001308"
     CDS             6578..7186
                     /locus_tag="IV329_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8940498.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            7206..7568
                     /locus_tag="IV329_001309"
     CDS             7206..7568
                     /locus_tag="IV329_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940499.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            7571..7753
                     /locus_tag="IV329_001310"
     CDS             7571..7753
                     /locus_tag="IV329_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940500.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            7770..11189
                     /locus_tag="IV329_001311"
     CDS             7770..11189
                     /locus_tag="IV329_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8940501.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            11240..11977
                     /locus_tag="IV329_001312"
     CDS             11240..11977
                     /locus_tag="IV329_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8940502.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            11987..14278
                     /locus_tag="IV329_001313"
     CDS             11987..14278
                     /locus_tag="IV329_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940503.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            14302..>15362
                     /locus_tag="IV329_001314"
     CDS             14302..>15362
                     /locus_tag="IV329_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940504.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGST"
BASE COUNT         5429 a         2284 c         3233 g         4416 t
ORIGIN      
        1 tagataaaaa agtatttaaa gctacgaagg aagttattca aaatatcgct acaaacagca
       61 aacttcgtta taacacggca aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc
      121 gctttgatga aattcacgaa aatgaagatt attcaatgat aaatgtggct acttctggtg
      181 gtggtaaaat tcgagattat agagaatttt atgatacaac taatggtcat gttcgtggtg
      241 gtccgcttga tgacattata gaagaatcaa aaatgattct ttctggagaa cttggaattg
      301 acaaggatgg agcagaattt tctagtttgt ttccatttat ttgtcgcttg gataacgata
      361 atgaagttga tgatcccgac atgtgggaaa aagcttgtcc aactattaat tacaatgcaa
      421 atttaaaacg gaaaatgttt caagaatact ctcaaatgca acgtaatgct ggtttaagac
      481 ttacgttcat gaccaaacga atgaacagac ctatggaaga tacacgattt gctgttgctt
      541 catatgatga tgttctgcat acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga
      601 taggaacagt cgattttgct gatagacgag attttgccag cgttgggttg ctaggaaaat
      661 acgataaaga tgtttatttt acacaacata cttttatcca cgaatcagcc cttcgattac
      721 aaaacatcaa acgagaggtt atagatattt ctatagatca aggaaaatca cagatcgttc
      781 atggaaaaaa tatagaagct gattatattg taggttggtt tcttgaaatg agtaataaat
      841 attatattaa aaaaatcgct atggatatgt accgtgcaaa aatattgaag cccgctttag
      901 aagaagcagg ttttactgtg gaaattgttc gaagcggatc tgttacacat ggtatgttaa
      961 aagatctggt tgatgacctt tttattaatc aacgtttatt ttttggtgac gatgcgatta
     1021 tgcgttggta ttgcatgaat gtatatgaag agcatatttc taatggaaat atacgctatg
     1081 aaaaaataga acctgaaact agaaaaacgg atggcttttt ttcattcctt catggtttga
     1141 attttttaga tgatatttat gattctgctc ctgtaacagt cacaaatagc tcagtagaaa
     1201 atacaggaac tggatttact cctctagtat tctaacttga aaggaggtga gaaagtgggg
     1261 atttttcaaa aggcggtagg atacttcaca aaaaaagcaa cggttccttt agaagaatac
     1321 ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat
     1381 ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa
     1441 aagaatgatc tttactatag gctgaatgta gctcctaata agaaaaataa tgcaacagaa
     1501 tttagaaaaa aactgatcag gagattaata ttctacaatg aagtattgat cgtttctccg
     1561 tctaataatt ctagcgaaat atttattgcg gatagttggg atgtcacaga atatgcattg
     1621 aaagatgatg tgttttctca agtgcaaatt aacaacatag tccttgatag agaatttcta
     1681 gaaagtgatg ttatctatat aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg
     1741 tattatcaag cgtatgggaa actcatttct agtgccatga atgtttacaa gcgctctaac
     1801 gctcgtagat acgtactgaa agggaattta ttccgaccgc aagacaatac aacacaagat
     1861 caaatcaata aaatgatgac atcacaattt aaggctttta tggaagctga taatgcaggt
     1921 gcggtatttc aattacaaaa tgagtacaca ttagaagatt tcagcggaaa cttccaaagc
     1981 aattcaagag atataaaaaa cttaatagac gacatctttg agatgacagc agcagcgttt
     2041 cacgttccga aaaacctact aaagggagac atgagtgggt tatcggatca agtggacgct
     2101 tttttaatgt tcgaaatcat accgattgct gaacttattc aggatgcgtt taacgctagt
     2161 ctctatgaag tagaagaata cttgtcaggg aattttgtac gtgtggatac aactatgatc
     2221 aagattacta gcttcaaaga tttggttgac gctattgatg taggcattag aaatggagta
     2281 tttacaatca acgaaggaag agaacgcgtt ggaaatgatc gctctgataa ggcgatggca
     2341 gatgaaatat ttataactaa aaacaatcaa caagtatcga aaggaggtga ggcgaatgac
     2401 gacaatgaaa acatttctag cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca
     2461 gggatttatt ggttctagtt ggttctttga agggaatact gacaagggaa tcaaaaatat
     2521 tttggatagt ctaggtgatc aagaagaaat tgaagtagta attaattcaa acggtggaga
     2581 cgtatttcaa gggattgcta ttgggaactt acttaagtca aataaagcaa aagttaacgt
     2641 tgtgattaac ggcttagccg ctagtgctgc ttcaattatc gcaatggctg gcgatactat
     2701 aaaaatctac aacaatgcac aattgatgat tcaccgcgct tccacatacg gagaaggaaa
     2761 tgtcgatgac ttccgtacga ttgctgacca actggaatca attgataaat cggtaaaggc
     2821 ttcatataaa acacgattca atggcacaga tgaagcattg caagaacttc ttgaaaaaga
     2881 atcgtttatg gatgcagaaa cagctttgag ttatggattg gtcgatgaaa ttatcgatgc
     2941 agaaaatagc tcaggtactg aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga
     3001 cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca gcagcattaa ataaaacatt
     3061 tggacaagga gatgtaaaat aatgacagtt aaaaatttaa aaggtgtaac agctgcaagc
     3121 gaccaattga tgaaagcttt taaagatggt aacgaagaat cttttagcgc agctatggta
     3181 agcttatcta aggaaattca ggataaaatt ttagaagaag caacagcaaa aaatcaagat
     3241 caattagtat taatgaaccg tggtcagcgt gtattaacta cacaagaaac aaaattctat
     3301 aacgaagtag tgaaaaacga aggttttgca ggggtcgaag aattagtgcc agctactgta
     3361 tttgaacgtg tatttgaaga tttagaacaa tctcatccac tattgcaaaa aattactttt
     3421 gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg
     3481 ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg gctttgatgt aattaacatg
     3541 aagcagttca agctatcagg ttatattcct gtatgtaagg caatgcttga tttaggtcca
     3601 gtatggttag atcgttatgt ccgtactgtt ttagtagaat cattgagaat tgcattagaa
     3661 caagcaattg ttgatggtac tggtaaagat atgccagtcg gaatgatgcg tgacatgagc
     3721 aaacaaacta gcggagaata tgctgaaaaa aaagcagaac ctattacagc tttagatgct
     3781 gtaactatgg gccgtttgat ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat
     3841 ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa ttgtgaatcc aactgattac
     3901 tggtctaaag tattcccagc taagactgta ctaactgcta atggagaata cgtacaagta
     3961 ttgccagtac cagtttcaga tttgcagtca acggctgtgc cagaaggaaa agcagttatt
     4021 ggggtagcct cagattactt catgggtgta ggatctacac taaaaattga agcttcagat
     4081 gaataccatt ttgttgaaga cgaacgcatt tatctagcta aacaatatgc aaacggtcaa
     4141 cctaaacgta acgatagttt cattgtatta gatattagcg ctttgggaac tactactaca
     4201 actacaaaac caacaaccac aacaactaca acacaagcgt aggtgatcag aatgaagtat
     4261 attctttgtc agccggcaat caatcggttt aaatgggagc ttgaagtttg tttaactaat
     4321 ctgaagaaac taggaatcaa agatatcgta ttgcttttca gcagacacga tgatcagatt
     4381 cctatttttt ttgagaagga atatggtgtt gaagttcatg tgtacgatga tctgcgggac
     4441 gacaaagagt atattccttc gattaaacca tatttatggt ggaaatattt agaagaagat
     4501 cattcgcgtg aggacgaccg atatttctat atcgattcgg atgtcatttt caataaaaga
     4561 attaatttgc gcaaattgcc ttctaaagat gatgtttggt attgtagcga ctgctgtagt
     4621 tatctaagtc ttgattatat tagaagctgt gaaaacggag aaaatattct aaaagatatg
     4681 gcaaacattg taaatgttac agtagaatct ttggaaacta taaacactaa ttcaggaggc
     4741 gcacagtggg ttattaaccg tcctaaagcg aattattgga aaaaggttta tctggattct
     4801 aatcggctat atcgctacct tagagggcaa aaaacaaata tacaaatctg gacagccgag
     4861 atgtgggcac ggctttggaa catgatgtat ttcaatattg gtcctaaagt tcacgaggaa
     4921 ttagactttt gttttgctac tgatccaata gaaaaagtta aagaagtaaa aatcttacac
     4981 aatgctggag taacaacaaa cgatgaagat ttatttttca aagggagata cgtgacttct
     5041 acgccttttg atgaagattt atcatttgta aacaagaaaa aatgctctta cgcatatgtt
     5101 aaagcaatta aggcggtggt tagatgacgc ctgaacaagt gactgaagaa ttgctaatag
     5161 ctgtgaagga taatatttac gttacctgga acgaagaaga tgagtcaatt aaaaagatga
     5221 tagctaaaaa tgctgtttat cttcaaagta aagtgagtac aacactttct ttttctcctg
     5281 aaagcttaga atacggattg ctaatcgaaa gatgtagata cgactggaat cgtgctttag
     5341 atgagtttga acaaaatttc gctagtgagt tattaggttt cattcaacat tatgcgctac
     5401 aagaatatat tgcaggtgat gtgaatggcg aataatcgta gactcgaaga aacattcaac
     5461 gatggttggt taaagatttt gacgcaaact accaaaagaa atgaactagg aaaaaagatt
     5521 ggtgtagaag atacagaaat cacttcttta aaatttagaa atctttccat gagagatagt
     5581 gatataacag ctatggatgc gatgggatcg aaattaacta agaaagtaaa gactccattt
     5641 catccaatcg ccaagaaatt taataaagat caatatttta tcgtaatcga tagtatgcgt
     5701 tacaacgtta tctatgccga ttacgataat ttttatatct atttttatct tgaaagtgtg
     5761 ggtgaatatg gtgattgata attctaaaga aaaagaacgt ttaaataagc aaatttctgc
     5821 tatcaaaact tccttagaag agcattttgg cctcaaactc tttcaagact ccgtaggcga
     5881 ggatgagcta cctgatgatt ttaattactt cattctcgaa acaggagaaa tagaaatgat
     5941 cactgagcca aaatatagcg tgggtcaaaa tctatatcta actttctatt cagaaaatag
     6001 agaagattta acaggagact cactagatat tatttcattg attcaaaatc gttcgattcg
     6061 ttttcagaga atggatccca atcatttaaa actagagaac caagatcgct atatcgatca
     6121 attggtattt acgtttagac gattattgaa gagtgattgt catggctaaa aatagttggg
     6181 agctaaaaat aaatggacat gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga
     6241 gcgaacgact gattaacgaa gcattgaaat caaaaggttc ggctattgca gtggatagga
     6301 ttacagaaaa aattcctgtt tctgaagcag atttaagaag agggcaccaa cacgcaaaaa
     6361 atagtcgtcc acttaagact caatacatta atttgggttt catcattaga cctacaagaa
     6421 aatttgagta tttaaaatat cctgatttgg ggataggtac ttctaaaaga aatcagccgg
     6481 acgaatttat gagaagagga ttaggtcttg cacttgatcc aattacagaa cttctgattc
     6541 gtcaattcga taaattaaat aaataggggg aacaacaatg gctaaaacaa aaactgtagt
     6601 aacaacgttc gataacgtaa gtatcaaacg aattgctttt aattttaaga acgcaggaaa
     6661 tgcaatcgca acgaattgta atggacaatt agatggcgaa acagaaatgc aaacggtggt
     6721 taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa ccaatcaata tgacggtaac
     6781 aattactgca catgtaccga tggaagttta tcgacgtttc aatgggttga aacaagatgg
     6841 acgtattaaa ccaggcattt actcttacgg tcctgattcc gtaggcgaag atttctcact
     6901 tgctgcagag atcgtggatg acttcgaaga aaatagaaag ttagttggta tgttagcatg
     6961 cacttcgaat acaggattaa cattctctat tgaaaatggt gcggatgaag tagctgcgtt
     7021 agaactagaa acaaaagtta tgcaagatga atttggtaaa ttttatcatg aagcaattgt
     7081 tgcagaactt gaagaagact taacagatca atggatgacg aatctatctg ctgatgtgat
     7141 taaaaaaact tcagttgtga caactacggc cactcaatca cagtaaaaaa aacggaggta
     7201 gcgaaatgaa cgaagattac tcaaaaattg aactaaacga tggaacaatt ttgaatttag
     7261 aacctaaact gaatatcaag aaattattga tgatcaatag agattttaac acagacgagt
     7321 ttgcaaaaat gactgtggga aaaggatcca tggatatttc tgttattcaa ggtgcaaagg
     7381 ctgtgtatat tgcttaccgc caagcgaaca tgactgatta tatttcattc gatgaattta
     7441 tcgataaatg ggattttgat atggctactg ccagctatat ttatcaattg atgatgttca
     7501 aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc
     7561 aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc gatgtctatt cgatgttgac
     7621 cgacgttttt tctatgcctt cagatttggt tttaagcgat atctgtttag atgacatttt
     7681 gcaaatggct tacaacaaga gtgcttatga aggatggaaa aactatgcaa taaaccaatc
     7741 tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa
     7801 ctttcatagc taacgatgac ggattgaaat ctacgttaaa agaaatcagt gctgaattaa
     7861 ctaaaaatag agcagaatta aaactagaac aagctcaatt acaacagact ggttctgaat
     7921 cagacaagtt aggaagtaaa ttatcttctt tagaaaagca gtatgaatta caaagccaaa
     7981 aagttgaagt aactagccaa cgtttagcca atgccaaaaa atattatgga gaaaattcca
     8041 ccgaagttca gaaacttgag agagaactga ttaaccaaca aacagcgcaa caacgtttgt
     8101 caaacgaaat tgataaaacg agtaatacac tagctcaagc aaaaggcgaa atacagacgt
     8161 acgagtctac aatgcaacag ttggatagtg aacaaaaaaa tgttcaagct agtgcttctc
     8221 tgattgaatc cgaatacaaa aaatggcaag taactgctgg tcaatcagct tctgaatccg
     8281 agaaattagc gaaagcccaa gaatatgttt ctcaacaatc tgaaaatgca gagaaaacga
     8341 tagatatcct gagacgacag ttagaagcta cacagtctga gtttggcgct acatccacag
     8401 aagcaatgca gatggaggcg aagcttaatg atgctgaacg tgaatttgaa gagttaggac
     8461 aagctgctaa aaatgtagat acaactaact tggacgatat cggaagcaaa atagatatga
     8521 ataatttaat ggaagcttct gacgttttaa gcgacattgg cgataagctt acagaattag
     8581 ggaaacaagc agtggactct gctaacagtg taggtagttc ccagagtaaa atacaagcta
     8641 attttggttt gactaaacaa gaggctgaag aattaacgaa tgtagccaga gacatttatt
     8701 ataaaggttt tggagaatcg ttagatcagt ccacagatgc attgattttg gtaaagcgta
     8761 atttaggcga tttaaataat caagatttac aaaatattac ggaacaagct atggtcctag
     8821 aaaacaccat gggcgctgat atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca
     8881 acttcggatt gagcgctcaa gatgcaatgg atttaatggt ttcgggtact caaaacggtt
     8941 tagataaaac gcacgaatta ggcgacaata tggcagaata tagccaatta tggagtcaaa
     9001 tgggatattc agctgatgaa acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt
     9061 ataaccttga taaagtcaat gacttagtta aggaaatggg aatatcgtta acagatggtc
     9121 gatttgagca aaacatggat atgtttagtg aaagtactag aaaagctttt gaagagtgga
     9181 aaaatggtgg aggaacacaa aaagacgtta ttaattccat gattcaagat tttagcaata
     9241 tggatggtca atacgaccaa ttaaataaag cttctacaat ttggtctgca cttggcgaag
     9301 ataacgcgat gaaagttgtc caatctttga ctgatgttaa ccatacattt gatgatgtta
     9361 gtggatctgc acaaaaaatg aatgaagatt ctactactcc gttacaagaa ttaaacggaa
     9421 aaatagctga attaaaggat tcattagctc ctataggcaa cacaatcata gatgcactcg
     9481 aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt taataatctt ccacaaccag
     9541 tacaggatta tgccgtagcg attggcggat tgactgctgc atttacttta ttaatgccaa
     9601 taatagttgg cttcatggct ctaggtggtc ctactacatt aataatagga gcagttatta
     9661 ctgctattgc tggagttata gcaattataa aaaactgggg cgcaattact gactggttta
     9721 agggaatatg gagtaaattc actgattggt tgggtggtac ttgggaaagc ataaaagaag
     9781 gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta gattgggttc
     9841 aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga ttagttgaag
     9901 gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt gattgggttt
     9961 caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa
    10021 ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt aatattatcg
    10081 tgggtatctt tgatgtagct ggacagttat taggcgcagc ttggcaagct gtttggacac
    10141 ctatttctga tttccttaaa aatacttggg atactatgac acaatggata agcatcgctt
    10201 ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg
    10261 gtatatggac atctattagt actacggctt caaatatttg gaactcaatt agtgcaacag
    10321 cttctagtat ttggaatagt atcaagaata caatcactag cttggtacaa gcagctgcta
    10381 cagtaattca aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa
    10441 tcagctctac agcatcaaat atctggagta gcgtgactag tagtataagc aatgctataa
    10501 acgcagctaa aagtgccatt caaagtgttt ggaatagtat atcttcgtgg atcagcggaa
    10561 tttggaacgg tatcaaaaac actgctttga atctttggaa tggaattaca agcactatta
    10621 gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta
    10681 ttgtttccga catattcaat aatgttaaaa gcacgatagc taatatatgg gaaggtatca
    10741 aaaagactgt tagcgctccg attgattgga ttagagataa aatcagtagt gtctttgata
    10801 atttgaatat ttctatacca catattccgt taccacattt taaattgagc ggagaattca
    10861 atccattgaa ggggaaaatc ccaacattgg gtgttgattg gtatgcgaaa ggtagtgtgt
    10921 ttaattctcc aaatattatc ggtgtaggtg aatcaggacc tgaagcagtt ttacctttga
    10981 aaagatctgt gctgcaagaa attggtgatc gtatcttgag tagcacctca gtttcatcta
    11041 ggacacaagc ggttcaacct gtgaacaatt acgaattcaa tttcacaatt gatggtaacg
    11101 cagatgaagt cactatgaag caaacaactc aacaaatcat tgatagcatt acaaaagttc
    11161 aaaatgataa tgcttcggca tggcgttaaa caggagagta tttctcctgt ttttttagta
    11221 ttaaaaagga tgtgaaaaaa tgactgattg tatacattct ataatcgatg gatttcctga
    11281 ttatttgcat aaattggctt tagctgaaag accaaccata ccttctccaa aaagacagag
    11341 agttgaaact tctgttttag gaaggttagg tggcttagtg caagattact cgtttgaaga
    11401 catgtcgttt acattgcact ataactattt agaggatgtg gaagaccatc aagcgttcaa
    11461 gcaatcgttt tatatcatgc gtcattggtt aaactatgca aagaaattag aattctctga
    11521 cgatcccaac gtctattacg ttatccagac tatcgatatt ggggatgcag aaaacgatat
    11581 tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga
    11641 agatgtacct ataaccgtag ataaaccaca gtcatttaac ttgctgaata atagtttaga
    11701 agaaagtttt ccaaagatta tcatcactcc ttcagctact tcatgccagt tcatcttaaa
    11761 tgattatgtg tttagtttcg aaggcttagt agcaggaact gatgtagtca ttgatagtga
    11821 tttgatgctt tgctacgaag agcaatcgga cggagatatt ttagatcggt ccaacaaaat
    11881 gaagaccatg caatatccga cattgcaagt ggatatcaat cattttaatt gcactggttt
    11941 gagcaaaata caaatttatc gtaacgggtt aaggtaggtg aaatagatga tcgataattt
    12001 aataactatt tacgataaaa atgacgcgaa taatttagct gaacatttat atgatacgca
    12061 aggtttaggc gctttgtcag actggttaac agctactgtt agcaataaac taaacggagc
    12121 cgagatattt cagggtactt atccaataag cggaactaat gcagatttga ttgtagaagg
    12181 acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta
    12241 tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa gctgaaccta ttttcaatga
    12301 tataagaaaa tcggtgttga ataaatatga cagcggaaca gaaaaaatca ctgctactca
    12361 ggcatggcaa aacgcaaaag ctttagcgaa accagctatc ccttcgcagt tttctttctc
    12421 gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa gcgaattttt tagaattctt
    12481 tggtggaaaa gagggatcta ttctagatcg atttcatggg gaatttctta aagataataa
    12541 cacattacat catgaaaaaa ggctaggtac ggatcataaa atcaaagcga tttatactaa
    12601 aaatttaact ggtcttgact tagagataga tgctcaaagc gttttagttg gagtttatcc
    12661 attcattagc agttcttcag aaggagaaga cgagatcact ctaccagaag aagtcatttt
    12721 cacggattac gtggatgatt atcctgctgg atatgtttct tttgttgatt ttaaagacaa
    12781 agcgactgat gtagccacat taagggaagc tgctaaagac tggttgaaaa caaacataga
    12841 taaacaaaaa ccacaagtga gtggttcgat tgaattagta ccattgaggc atcaaagagg
    12901 ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt gacggagtag atgtgtatca
    12961 tccacagtta aaagtgaata tgtcagcgag aattgtggaa tatacgttta atgttctaac
    13021 caattcatac gataaattag ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga
    13081 gaataatgta agcaatttga ttaatgatgc cattgatcaa ttgaaaaatg gtggcgaaat
    13141 cagtgattta atcaatgata ttgtagatca tcaaactgat atgattactg gtcaaaatgg
    13201 tggttatgtt ttattagatc ctaaagaagc gcctagtcgt attttgatta tggacacacc
    13261 agataagaat accgcaagga atgttttaca aatcaacaat gctggtattg gtttttctaa
    13321 aactggcatt aatggaacat atgaaaccgc atggacatta gatggcggat tcaatgcctc
    13381 gtttattacg gctggtgaaa tagtaggaat tactattaga ggtactacat taattagtga
    13441 tggcactgat tatagaacaa gtattgctaa tggcaaaatg acttggtact caaaaaaagt
    13501 taacaaagat attatggagc tagaagcacg tgattatgta agtgctgatg ccggtattgt
    13561 atcatacacc atgaaaactg gtggtggttt catgattaga aatccacagg gtaacttggt
    13621 ttttagtacg tgggataatg gtaataacag accgtttcta tcttttggtg cgcccaattt
    13681 caggtatagc aatgctagtt atgtaacttc tggcgacggt agttctttaa gcattaatgg
    13741 tagtgcgggt aactcatggg aatttaaggt agctggtagg actatgaaat ttactagtga
    13801 tggtatgcta acgttgccag gttgtttttt tggttcatgg gaagatggga aacttgctag
    13861 gtttgaacaa tcaacggtac aagtatataa agattttact gttagaggta ctaaaaactc
    13921 aaccgtacca acagaacatt atggacaacg actattaaac gcttatgaaa ctccagaata
    13981 ttatttcgct gattatgggg aagccgttac aggtgatgat ggtaaagttc gtgttgatat
    14041 tgaccccatg tttgctgaga cagtaaatct aagtcgatat atgacacatg tgacacctac
    14101 agaactagtt ttgtgtgctg ttactcatga agatattgac catttcatca ttgaaactag
    14161 taagccaaac gtattagtta gatggaattt agtggcacac cgtctagggt atgaagatat
    14221 tagattaaaa gaggatacag catatgatag cacagtgctt gaccaaaaac gtttttaaaa
    14281 cgaagacaag gaggtatata aatggctagc agtttatata atttggctct agatttcagc
    14341 aaagaattaa actacaccaa agctattatg gctcgtcaag gtgataaagg gattacagtg
    14401 actgttaaac catttttaaa tggcttgcag atggatacga gtggcggaac atttacttta
    14461 aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg caactagtgt aactagtgaa
    14521 gaagtcacgt tttctcttga tggcacattt atgagtgaag caggatatta taaacactgc
    14581 tacgtagaat atagaaaaga caatcaaatt ttaacaacgc aagatatcat ttttttctca
    14641 ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat acgtttcgca attggaagag
    14701 ttgattcaaa agtataacga aacttttgat gcttttatgg ctgaaatcga aggtagagtg
    14761 gacagcttaa ataaacagat tactgattta actggtcaag ctaaaacact acaagacaag
    14821 ttagacgctc tgaaagaaga aatttctaaa ttaggtaact tgcaggtgat gtacagtaac
    14881 agcatcgact tcgggggata tgattatagc ggtaagccga atttaatgtc caaactaaaa
    14941 tcgagcgatt ttaacgttgg ttaccacggg tcactaactt cggataacga aaagctacat
    15001 tttacttctg atggtacagg aagcattatt atgtttacgc gtattaatac acctcagctt
    15061 gctagtggga aaacctatac tctgagtgcg aaagttcgat ttgatgaagg aactacagga
    15121 gctattgata aattacgttt ggtgtatcgt acatcaccag gagaaaagat attattggaa
    15181 gcaaatagta caaatattac aacagatgat gtagggaaag aaataacaat caaaggtaca
    15241 gctaacgtta attatcaaat cacaaattta gatcgatttt atatgagtat tagctttgtt
    15301 gacagggata aaataaatgg cggatttaag ttgtacgaca tcaaaatcga agaaggctca
    15361 ac
//
LOCUS       DACDKL010000056        15361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDKL010000056 DACDKL010000000
VERSION     DACDKL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.056"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            95..373
                     /locus_tag="IV329_001315"
     CDS             95..373
                     /locus_tag="IV329_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940505.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            392..1600
                     /locus_tag="IV329_001316"
     CDS             392..1600
                     /locus_tag="IV329_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8940506.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1748..2113)
                     /locus_tag="IV329_001317"
     CDS             complement(1748..2113)
                     /locus_tag="IV329_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8940507.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2260..3033)
                     /locus_tag="IV329_001318"
     CDS             complement(2260..3033)
                     /locus_tag="IV329_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8940508.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3207..4067)
                     /locus_tag="IV329_001319"
     CDS             complement(3207..4067)
                     /locus_tag="IV329_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8940509.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4067..5485)
                     /locus_tag="IV329_001320"
     CDS             complement(4067..5485)
                     /locus_tag="IV329_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8940510.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5542..5991)
                     /locus_tag="IV329_001321"
     CDS             complement(5542..5991)
                     /locus_tag="IV329_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8940511.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6436..6699)
                     /locus_tag="IV329_001322"
     CDS             complement(6436..6699)
                     /locus_tag="IV329_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8940512.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6798..8093)
                     /locus_tag="IV329_001323"
     CDS             complement(6798..8093)
                     /locus_tag="IV329_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8940513.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8090..9361)
                     /locus_tag="IV329_001324"
     CDS             complement(8090..9361)
                     /locus_tag="IV329_001324"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8940514.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9526..10395)
                     /locus_tag="IV329_001325"
     CDS             complement(9526..10395)
                     /locus_tag="IV329_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8940515.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(10687..12297)
                     /locus_tag="IV329_001326"
     CDS             complement(10687..12297)
                     /locus_tag="IV329_001326"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8940516.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13490..14830)
                     /gene="glnA"
                     /locus_tag="IV329_001327"
     CDS             complement(13490..14830)
                     /gene="glnA"
                     /locus_tag="IV329_001327"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8940517.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(14858..15250)
                     /locus_tag="IV329_001328"
     CDS             complement(14858..15250)
                     /locus_tag="IV329_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8940518.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4358 a         3275 c         2640 g         5088 t
ORIGIN      
        1 taagtacctt tttccatcac ttatgcgatt tttttccttt tttttattcg ttatactgca
       61 gaagtaaaat tttttaagaa tagagggaaa aaacatgaat tactggatga acacaatcat
      121 taatcgatta gaaacagcat atcaaacaag atttgatatg aaagcttctt tagtcttttt
      181 aaatgatgcg tatcaaaact cgatcgaatt gatcaaagca gtggatgaga atccaacaaa
      241 cgaatgcgaa gaatttttaa atctgtttat gtctacaaga gatttattta ttcgccaact
      301 tgttgatcgc tatccatcca attatcacga tgtagaagtt caaatacaaa aattaaaagc
      361 atattcagct taaggggaat gtataacata catggaaaag aaattattta ataaatattt
      421 tattggaatc acattgatca attttattgt ctacctgatt tattatctgt tgatggtcat
      481 catggcagtc attgcgcaag acaccttaca cgccagcttg agccaagcag gttttgcttc
      541 tgggatctat atcatcggaa cattgctggc acgcttattc atggggaaac agttagaact
      601 atttggtcgg aagtttacct taagaggcgg cgcgatcttc tatctccttt caacattagc
      661 atatctttat acgccaacta tcggggcatt gtatttagtt cgattcttga atggattcgg
      721 ctacggtaca gtttctacag ctacaaatgc tatcgttact gcatatatcc ctgcatctaa
      781 aaaaggggaa ggcatcaatt attatggatt aagtactagt ttagctgccg ctgttgggcc
      841 atttttagga atgattttgc tgaatatgac tgattttcgt ttcattatct ggttttcaat
      901 tgtactggtc ttctttgtta cgattgcctg ccttgtcttt cctgtaaaaa atattccatt
      961 atctgaagaa caaaaaatat ctcttcgctc atggaatatc aatagtttta ttgaaaagaa
     1021 agcacttttc attacaggaa ttgccttttt gatgggtctt tcttattcaa gtgttttatc
     1081 atttttatct tcttatacaa aagtaatcca cttagttgct gctgggtcat ttttcttcgt
     1141 cgtatatgct ttggtcatca catttacacg tccattgact ggaaggattt tcgatgtcaa
     1201 aggagaacaa tacgtgatgt atcctagcta tttattctta acagcaggat tatttttact
     1261 gagtgtaacg acgaatagtg tgacattgct tatctctgga gcattagtcg gcctaggtta
     1321 tggtacgttt atgtcaaatg gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat
     1381 cagtattgct ttatctacct actttattgg tttagaccta ggcttaggcg ttggtcctta
     1441 tttgatgggg atgctaaaat ctcttacttc atttagagga ttgtatgtga ttgccggagt
     1501 cattccattg atatgtacag cactctatct tgttaatggc cgaaaacata gtcatgaagc
     1561 agagggcgaa ttgattgaat cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa
     1621 aaaccgttca gatctgaaaa taatctgagc ggttttttga gtaaaaatag tttatgatta
     1681 ttcttaatta aacatctatt tatttttaaa gctttcattt atcgataaga atagtttaac
     1741 tgttgattta ttccattttc cgttcatctg gtacgatgcc ttgacttagc aaccacttgt
     1801 ggaagaacca ttctgccaca ccgatcaata gagcaggaat caatactttc atccaaatat
     1861 cgactgtatc attcataaaa tatagataga gccacaagat aactgcagct gaaccagcat
     1921 ctgcgatcgt cgctgcgatg ttccctactt tacgtaagat caacagatcc cctagcacat
     1981 agatcatgac tacaataatt accccaacaa tcgttgaatc cataaagcta acaccgtata
     2041 aacctgtcag cacgacccac agaatcgccc agatcatgat tgccttcact accaacgcct
     2101 tcacatgttt cattttctgc actcctttct cctggataaa aaagtaagat tatctaacaa
     2161 gataaccttt tcattattat accatcggct gagagaagcg agcaaagata aggtcctatc
     2221 taaaaatgag gaagccactt agaacattca cgtaataatt tatccgtaca gacattcgat
     2281 atggtgttcc tttactttcg catactgttc ttttgtcagt tctttacttg tcaccagata
     2341 atcgatttca tgaaaagcaa tggcttgatg catgaattta tgtgcaattt tttgctgtgt
     2401 aactaaaacg gcaagccggt ctgattgttt tctaaaagct tttttgacca ttgcctctcc
     2461 ttcatgagtt tctgttactc cttggctgaa atctagcccg cgacaactca ttatcgctaa
     2521 atccgcataa tatgacaatg catcgttata ggcttttgaa ccgttgattg ttgctcgttt
     2581 cggaacaatt gcccctccca atacagatac ttttgccgtt gtatattcac tcaacatagc
     2641 agctgtgacg atgccattcg taatgatctg caaaccatca aatcttgcta ggtttctgac
     2701 taagtgtaaa gaggttgtac tggaatccaa gaataagacc atatcatctc gaatcaaaga
     2761 agaagctgta tctgcaatct tacgtttttc tgtttcttct tctaatttgg tgatcttcat
     2821 caggtaaggc acgtcaattt ttcgattgtc aataagcatg gcgccaccgc gaactctacg
     2881 gatcaatcca cttttttcca gaaaaacaag gtcacgttta atcgtggctt cgctataatt
     2941 tactaactcg atcaaatcgg tgactcttgc aaattggttg ttttttagat aattcacaat
     3001 ctcctgctgt ctattctcca cttcattcaa cataaaatca ccccatttac tttatatagt
     3061 aacaggaaaa aagaggttgt gacagaagtg attggcttcg agaaataaga agaaattttt
     3121 cgaaaaaact acttctgaac atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat
     3181 accctcttga ccacacattt ttatttttag tataaactag ccttgcctac tgtgttcaaa
     3241 atttccattt tatgtttgac cacttcttgt acctttttat tagctgctgg ataaattgca
     3301 ttcggctcgt acatcgatgg attttcactc aatatttctc tcaaagcatt gaaaaagacg
     3361 cttttaagat ctgagctcaa attcactttg gcaactccgt attttactgc ttcgctgact
     3421 tctgcgtccg gattacctga accgccatgt aaaacaaatg gaagatcgat ttgttggttc
     3481 aattctttca gtaagtcgat atttaatttt ggagttctgt ttttcggata caagccatgc
     3541 gctgttccaa ttgctacagc aagtgtatcg attcctgttt ctttcacaaa aatcttcgct
     3601 tgatcaggat ctgtataaat gattgtttca gcacctcctt cagcagaacc attattccca
     3661 atcgttccaa gctcagcctc cactgagaca tttaccgcat gagctaacgc aaccactttt
     3721 ttcgtaatct gtacattgtc gtcgaatgat ttcaaagacg catcgatcat cacggaagaa
     3781 tagccgtttc gaatcgcccg taagacatct tctatcgtac cgccatgatc caagtggata
     3841 acgatcggca gtttacttgt ataagcatag gctcttactg ccgcaacaaa ctcatcactt
     3901 aaataggcga tttcatcagg atgaatttct aagattactg gtgcttctaa tctctctgct
     3961 tcttcgatga ttgctttcat catatcatag ctgcaaatat tgaaggctgg tactgcaaat
     4021 tgattctctt tagccacttt caataactct ttcattgtat ataacattac gcttccacct
     4081 catttgttag attttcattt ttcgtccact tgtcttgttt tttctctttc actgtttttt
     4141 tcaatagtcc aagcataaag gctgtagtga ctgtacctga tgcaattgcc aagacataca
     4201 taggtaaatt gttcataaca tttggaatta gcatcaccca aatccctcca tgaggtgcca
     4261 ttgaagtaac tcctactgcc atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac
     4321 taggtatcac gcggatcggg tcagataccg caaacggtat agcaccttct gtaatatacg
     4381 aagcgcctaa aatccagcat gattgacctg aagtccgttc ttctttggta aatttatgtt
     4441 tgaataataa agtggctaaa gcaataccta ggggaggtgt cattcctgca gccatacaag
     4501 cagcaatcgg tgcatttact ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt
     4561 tattgatcgg cccacccata tcagctgcca tcatgatccc gataagtaat ccgaacaata
     4621 gcccggatgt atttcccaag ctattcagcc aatttgtcaa agcatccaac acccatgtga
     4681 tcggttgttc aatcacaaat accatcaaaa gcccaataat taagatcgat aaaaagggga
     4741 cgacaatcag ctgataaata gcagaaaatt ctttttttac gctaatttta tttgccagaa
     4801 aatcacagat ataccctgct agcacgccac caatcattcc gccaagaaaa cctgaaccac
     4861 ctgtagaggc cagtaatccg gcaacaattc ctggggcaaa acctgctttt ccagcaatcg
     4921 atgtagcaat ccccgcgcct aacgcaggaa ccatcatggc aaacgctgta tctcctccga
     4981 tccttgaaaa tgcagctgct aacacattat agtctgctga gttgggatca gccgctgtaa
     5041 tcccaaaggc aaaactcagt gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt
     5101 aattcacacc attcatgaaa tgattataga ttgtcggctt tttctcattt gcagctggtt
     5161 ctttttcatt agacacaacg aatacgccaa tatctctcaa tgcttcgtct attactactt
     5221 gcggttcttt gattgctcgg cttgtagaga cacgctttac ttttttcccg gcaaatcggt
     5281 ccaattcgat ttctttatct actgcaagaa tgattgcatc tgcctctttg atttcttgtt
     5341 cagataactg attttctgtt tttacacctt gtgtttcgaa ttttgcttga tggtttagat
     5401 ttcttgctgt tacttctaat ttttctgccg acatataagt atgagcaatt ccagtcgggc
     5461 aagcagttac accaacgatt ttcatgatta ttttcctcct ttttcttcac tatcgatact
     5521 cggacaaaga ctctactatt ttcattcttg actaatctgt ctgatgatat ctgctggcgt
     5581 agtggcttct tttattccct ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct
     5641 gagaatctct agatgcatat ctgctgattt ttcaggaact acgatggcaa aaatatattc
     5701 aattggcatt tcatctatcc cgctatagtc cgttaatttg gctgtatgaa aaaaacacag
     5761 tgctggtttt gtcacatatt tgctttttgc atggggaatc ccaaatccat cttctattcc
     5821 tgtggagatc tcctcctctc gtttgaatag tgccttaacg actttttgaa catttcttac
     5881 cttatttgtt tttttcaaca catcccctat tcgctcaaat aatgatcctt ttgaatctat
     5941 cgatggatcg aaaacaatca gttcttctgc gatcaattga tcaaacctca taaaaaacct
     6001 ctcccccaca aaaaatgtta acgttttctt ggaactgagg tcagtatagc atcgctagct
     6061 gtaccgttca atattatgaa tgaacagagg aaaaagaccc atcatccgcg tgtataagtc
     6121 attaaaatga acgatctagc tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt
     6181 ccttgaaagg aaagcttagc ttctaatttt aggtaaatgg ttttttactt cgaaataacc
     6241 cctcttcatt tcgttaatat aaggtgtttt tatcaagtgg ataattaaaa ctatatacta
     6301 gtttttcatt gttttgtgca ttatgatcct acgacttttt cagcatcaaa cgaagtttta
     6361 aattggcaaa aaacataaaa aaacagacta tccctatcag atagtctgct aaacgttgag
     6421 ttatcagcgt ttttattatt ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa
     6481 acggtccaca cgtccgtctg cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga
     6541 gtcagaagtt acttcgacac ggatgactgg gtatgtatta ccgtcttccc attcaactgt
     6601 ttcttgtgaa gtttttgttg aacctgacaa gaatttgaaa ccagttgttg aatccatgaa
     6661 tactactggg tggtaatctg gatggatgtt ttctttcatg attttttcgc tcctttgccc
     6721 tgctccattt gctgaaccag agttgatttg tcgatttaca acattaacat cttaacatgt
     6781 ttcagtcaag gttgcaatta tcttttcaga tttctggtcg gatttttttt accaaaggag
     6841 acatcttgaa attctttgaa aaattgctga ttgttttttg ttttacgcaa aaagcggatc
     6901 agttgttccg tatattctaa tgaatctcca gtcatatttt ttcgaagttt ccaaatttct
     6961 tctaattctt cacttggcat caataattct tcttttcggg tacctgattt tttgatatcg
     7021 atggctggga agatccgacg ttcagataat tcacgtgaaa gttgtaattc tagatttcca
     7081 gttcctttga attcttcata gatcacgtca tccatacggc ttcctgtatc gactaaagcg
     7141 gtcgctaaaa tcgtcaaact gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc
     7201 ttaggtttat acaatgcggc tgggtcaatc ccgccactca atgtacgtcc gcttggtgga
     7261 acgactaagt tataggcccg agcaagtcta gtaatactat ccatcaagat aacgacatct
     7321 cgtttatctt cgactaaacg cattgcgcgt tccaatacta attcagaaac tcgagtatgg
     7381 ttttgcggct gcagatcaaa tgtagaagat accacgtcgc ctttcacgct tcgttctaga
     7441 tccgttactt cttccggtcg ttcatcaatc agtagaacga ttaattctac ttccggatag
     7501 ttttcggtga taccatttgc gatttctttt aatacagaag ttttccctgc ttttggcggt
     7561 gcgacgatca atccacgctg accaaaaccg attggagaaa agatatcgat cattcgggta
     7621 gatagtcttt gcggtgttgt ttccagttga agctgtcttt gcggataaag aggtgttaag
     7681 gctggaaaat gcggacgttc ttttgcttct tccggatctt ttccattaac gctttccacg
     7741 tgcatcaaac cataataacg ttccgattct ttaggtggac gggcttttcc tgccactttg
     7801 tccccatttc tcaaactgaa tcgtctgatt tgtgaggcag aaatatagat atcttctgca
     7861 cttggtccat aattgattgg ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat
     7921 attccttcca tataataaaa tccttgtttc tctgcttgtg cacgaatcac agctagagat
     7981 aattcctttt tattcatctt actgtaataa ggaattttaa attctttggc ataactataa
     8041 acttctttta acgttttgtt ctctagttct gccattgtca aataatcgct cattcagctc
     8101 ccacttcctc tatcatttca attgatgccc ctaaggctgt tagtttctcg ataatatgat
     8161 cataaccgcg taagatataa tctacattgt aaatcgtcgt tgtcccttct gccaatagtc
     8221 cagcaatcac taaacaagca cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa
     8281 gtttatttgg accattcacg atgatcatat ttccttccac ggtaatatca gcgcccattc
     8341 gtgctagttc cggcacatgg ttcacacgtt tcgcatagat cgtatcgatg atttctgaag
     8401 tgcctgtagt catcaatagt aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg
     8461 gataaggata ggtcatgatg tttgcgggtt ttaattcttt cgaggggtaa acttcgattt
     8521 catcttcgct gattttcata tggacaccaa tttcctgcaa ttttgctata aagctttcca
     8581 aatgttcaaa gatcacattt ttcactttga taccatttcc tactgcagct gctaatgcta
     8641 gataggttcc tgcttcgatg cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt
     8701 cgactccttc gatacggatc acatctgtac ccgctcctcg aacttttgcc cccatattat
     8761 tcagtaaagt tgccacatct atgatttccg gttctcgtgc agcgttttca attactgtct
     8821 gtccttttgc ttttacagca gctaacatca cattgatcgt tgctccgact gaaaccacgt
     8881 ccatgaaaat ccgattgcca cgcaatcctt tattttctgt ccgtaaatac atggcaccat
     8941 gttcatttgt cacttcagca cctagagctt caaatccttt gacgtgtagg tcaatcggtc
     9001 tagggcctaa ataacagcca cctggcaggc ctactaccgc ttcgccaaat ttaccaagca
     9061 gtgttcccat aaaataataa gaagcacgca aactattgat ttttccgtgc ggcatcggaa
     9121 tcgatacgac ttctcgagga tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc
     9181 ccatgatctc taaaatttca attaatgaat gaacatcttg tatgtccggc acaccttcca
     9241 agatcactgg ggaatcagct aagattgcag ctggaatcaa tgcgactgca ctattttttg
     9301 caccgctgat cgttacttca ccagctaaag gtcggtttcc tttgattacg atttttttca
     9361 tctgtttcca ccttgtttat taatcttttt tcatggatat gcttgtattt tatcatagat
     9421 aaaaaaaaga aacgagcatg gtctgccgtt ttaagtaaaa aagaatggag agcgacttca
     9481 aaagtcgttc tccattccta tatatcgtat ctgttatgtg gatgcttacg ctttgttagc
     9541 tgaaccgaac cagtcgatat gagatttagc atcttcaacg attgctgctt tacctggagc
     9601 taataattta cgagggtcga atcctttacc ttctttatct ttaccagctt cgatatattt
     9661 acgagtagct gcagcaaatg ataattggaa ttcagtgtta acatttactt ttgagatacc
     9721 cattgagata gctttttcga tttgatcttg aggaatacct gatccgccat gtaatactaa
     9781 aggcatgtct ggacctacag catcagcaat tgcttgtaag tggtcaaaag ctaatccttt
     9841 ccagttttct ggatattgac catggatgtt accgataccg caagctaagt agtcgatacc
     9901 tgtagcaacc atttgtttgc attcttcaat atcagctaat tcgccgttac cgatgattcc
     9961 gtcttcttct ccgccgattg aaccaacttc acattcaact gagatacctt ttgcatgtgc
    10021 tttttcaaca acgtctttag ctaatttcaa gttttcttca aatggtagat gtgaaccatc
    10081 gaacattact gaagtatagc ctaattcgat acattccaaa gcagcttcgt aatcaccatg
    10141 gtctaagtgg atagcaactg gaacagtgat gttcattgct tcaactaaat cagtgattaa
    10201 atctttacag attttgtaac cacccatgta tttagctgca cccattgatg tttggataag
    10261 tactggtgct tgttttgctt cagccgcttc aaggattgct tgagtccatt ccaagttgtt
    10321 tgtgttgaat ccgccgacag cgtaaccgcc tttacgagca gcttttaaaa attcagctcc
    10381 tgatactact ggcataataa aattcctcct aagttttgta aaactaaatt tgttaagaca
    10441 attcttaacc aacgacccta ttttaccaaa catttcggta gttttctact aaaatcaatg
    10501 aaaatgaaaa ttttcaagaa gttttttgca aaaaataaaa tatccaatag cgctttaatc
    10561 gaaaaaagtc ttttgctttc tatctttttt cctacaaata ataacaaatc agaaaaagag
    10621 aaaagtgaca agaaaattgc ttatcccctt ctcttgctac ttttctcttt ctatcaagtt
    10681 caaaaattat tttgtttcgt gatctgctaa tgatgctccg acaaatgctt tgattaattt
    10741 ttgcgggcga tttggacgag aaatcaattc tgggtggaat tggcatccca caaagaattt
    10801 tttctctggc agctctacga tttctactaa acggttatct ggtgagacac ctgaaaagac
    10861 tagcccttgt tcttcaaaca attgacgata tttgttattg aactcatagc ggtgacggtg
    10921 acgttcttgc acaacatcgg ctccatcata tgcttcagcc gtcttcgttc cttttttgat
    10981 tttacatggg tataatccaa ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc
    11041 agccattaaa tcaataatat tgtttgctgt atctggattc gtttcagctg atgacgcatc
    11101 ttctaatcca acgacgttac gtgcaaattc gacacaagcc atctgcatac caagacaaat
    11161 acctaaaaac ggaacgtcat tttcgcgagc ataacgaatc gcttcgattt tcccttctac
    11221 tccgcgatca ccaaatccac caggaaccaa gattccatct gcgtctttca aacgttcttc
    11281 tacattttct ctcgttaatt cttgtgcttt gatccaatcg atctcgatgt ctgaattata
    11341 ggcgaaacca gaatgtttca atgcttcaac tactgagata tatgcatctg gtaactcgac
    11401 atatttaccg acaagcgcaa tccgtacttt tttcttcaag ttcaagacac gttcttctaa
    11461 ttctttccat tctgtcatgt ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat
    11521 ttgatccatt ccttgtgctt gtaaaactaa tgggatggag taaagtgtat ctacatctgg
    11581 cgactcgata actgcttctg gttcgacatc acaaaattgt gccaatttgt ttttcacgct
    11641 ttgagaaaca ggttgctctg tacgtaccac caaaatattc ggttggatac ctaaaccacg
    11701 tagctctttt acactgtgct gcgttggttt agttttcatt tctccagcag ctttcaagta
    11761 aggaatcaat gttgtatgga tatacataac attatctgta ccgacatctg ctttcatttg
    11821 acgaagtgct tcaaggaatg gcaaagattc tatatcccct actgttcccc ctacttctgt
    11881 aatgatcaca tctgaatcag tcattttagc tgctcgcatg attttttctt tgatctcatt
    11941 tgtaatatga ggaataactt gaacagttgc tcccaagtat tcgcccttac gttctttacg
    12001 caaaacttca gaatagattt ttcctgtcgt cacgttggag tatttgttca aattgatgtc
    12061 aataaaacgt tcgtagtggc ccagatccaa atctgtttca gcaccatcat ctgttacgaa
    12121 tacttctccg tgctgatatg ggctcatcgt ccccggatcc acgttgatgt atggatcaaa
    12181 tttttggata gttactttta agccgcggtt tttcaataag cgtcctaaag aagctgcaac
    12241 aatccctttt ccgattgatg aaacgacacc gccggtcaca aaaatatatt ttgtcataag
    12301 ttcaaaaact cctttttaat ttatgtgcag gaaaagtctc agacaataaa caaagctccc
    12361 tattcttcaa taagaatagg gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt
    12421 gcccaaggtg tataatacaa gctgactaag ggaaagtcaa gtactcaaat gaatactatc
    12481 cgcttacaat tttttctttt tcgatatcct ttgagaaatt atttcttaag tcctttatga
    12541 gtgcaaaaaa aatttaaccc acataataag cttgatataa acatagctaa cgttaaacct
    12601 aagcaaatca gaaaatcgtt actgaaaaat tttaagtgaa ataatagata aaaaaataaa
    12661 acaaacatta tgatagataa tatcccacct aacaaagaac tactttttcc tacttgcagc
    12721 aacagtttat atagcttatc gtagaaacaa tctttttctt tcacccttat ttacctcctt
    12781 tacgtaaaaa acgtagtcca cagttctcgg atttttggca tatcttttct attaaataat
    12841 atcaaaaaca aaacaattcc actaattttt tcaaagatga cctttttttc aacaaaagtg
    12901 ccttgtaggg attcattgaa ggtaacgaat cacataagta aaaaataagg agatgcgaca
    12961 aaagtcgtgc ctccttattt ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa
    13021 gtcacaatat ccatcaaaca tgagaagctg tttttgaaat tatttcttgt gattcggagt
    13081 taaatggtag gctcacaacc tctggataaa cggtattcaa aagccagttt ctcggtatta
    13141 agccaaattt atgcaaaaat gtagaaagcc attttcacaa aatttttctt aatactcgaa
    13201 actaaacgtc tttttcaaaa cctcttggta aacggtgttc aaaagcttgc atctgcggta
    13261 ataagttcag ccaattcaaa aacaagaaaa gctgtttttg cttggctatt cttattactt
    13321 gttgcagggc agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg
    13381 gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgttagtt tctctaatac
    13441 tcaggtcaaa acgctgattt cacagcctct aattcacggt attcctgttt taataaagtt
    13501 ctagatattg ttctctttcc cactcggaaa cagtttgtct gaatgccgcc cattcgagac
    13561 gttttgcttc aacaaaattt gagaagatat gttgtcctaa tgcgtcaatc attacattat
    13621 ccttacgcaa ttctttgata gcattatgga tagttgaagg taagtcctgg atatgtgcag
    13681 cttctctttc ttcctcatcc attacataaa tgttacggtc aacagctgct ggcggtgtga
    13741 tctgattacg gataccgtct agtcctgctt gcaacaatac tgccattgcc aagtatgggt
    13801 ttgcagatgg atcaactgac cgcaattcca aacgtgtgga taaaccgcga gattctggta
    13861 cacgtactaa tggcgaacga ttacggccac tccatgcgac ataaacaggc gcttcataac
    13921 ctggaacgag acgtttataa gaattgactg ttggattgca aacggctgtg taggcacgag
    13981 catgtttcag caaaccacct aagaaatgat aagctgtttc acttagttcc aattcaccgt
    14041 ctttatcata gaaaacattt ccttcatcat tgaacaaaga catattgcaa tgcataccag
    14101 aaccattgat cccaaataac ggtttcggca tgaaagtcgc gtgaagtcca tgcttgcgtg
    14161 caatcgtttt gacaacgagc ttgaaggttt ggatattatc acaagcatct accacatctg
    14221 catatttaaa gtcaatttca tgctgtcctg gcgctacttc atgatgtgaa gcttccactt
    14281 caaagcctag actttctaat tccaacacga tgtcacggcg gcaattttca cctaaatctg
    14341 ttggtgcaaa atcaaaataa ccacctttat cattcaaggt cgttgtgatc ccaccgtctt
    14401 cgtctaattt aaataagaag aattctggtt caggtcctaa attgaaagag gtaaaaccaa
    14461 ggtcgcgcat atctttcaat gctcgtttca aattcccgcg aggatcgccg gcaaatggtg
    14521 tcccatccgg attatagata tcacaaatta aacgagcaac ttttccatga gcactttccc
    14581 acgggaagat catccaagtg gagagatctg gatacaaata catatcgctt tcttcaattc
    14641 gaacaaatcc ttcaatcgaa gaaccgtcaa acatcatttt attttctaat actttgtcga
    14701 tctggctcac tggaacttct acatttttga tcgtacctaa gatgtctgtg aacatcaatc
    14761 gtaaaaatcg tacgttttct tcctcaatga ttcgtttgat ttcttcacct gttatctgtt
    14821 tctttaccat ggtatccctc ttcctttttt aatttattca gtcaaagaat tgtctttgca
    14881 atccttgcga ctgataatga cctgttttgc ttaacccacc ttgcgccaaa atctcatcac
    14941 gaaaaatttg tcgaacgtca tgatctgtca acggcttctt gttttttgct tcttgttttt
    15001 tcgcctgtgc catttcgtat acacgtttga tcccagccat attcaatccc tctgaaagat
    15061 agtctttaat ctcaagcaac atatcaatat cattcagcga atacatacgt cggtttcctt
    15121 cgcttcgttc cggatgaact aaatcttgtt cttcataata gcgaatttgc cgagcagaca
    15181 aatcagtcag cttcataact gtaccaattg gaaatacgga catagatcgg cgcaattcct
    15241 tttctcccat cagacgtccc ctttcgtttt ataagttggc taaagtgtac caattaaaaa
    15301 aaatattgtc aactgcatat gttaggtttt ctaacatgag atctctattt tttataaaat
    15361 a
//
LOCUS       DACDKL010000057        15165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDKL010000057 DACDKL010000000
VERSION     DACDKL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.057"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(145..525)
                     /gene="yajC"
                     /locus_tag="IV329_001329"
     CDS             complement(145..525)
                     /gene="yajC"
                     /locus_tag="IV329_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8940519.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(622..1767)
                     /gene="tgt"
                     /locus_tag="IV329_001330"
     CDS             complement(622..1767)
                     /gene="tgt"
                     /locus_tag="IV329_001330"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8940520.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1985..2683
                     /locus_tag="IV329_001331"
     CDS             1985..2683
                     /locus_tag="IV329_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8940521.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2851..5490)
                     /locus_tag="IV329_001332"
     CDS             complement(2851..5490)
                     /locus_tag="IV329_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8940522.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6109..7140)
                     /gene="queA"
                     /locus_tag="IV329_001333"
     CDS             complement(6109..7140)
                     /gene="queA"
                     /locus_tag="IV329_001333"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8940523.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7333..8508
                     /locus_tag="IV329_001334"
     CDS             7333..8508
                     /locus_tag="IV329_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8940524.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8512..9153
                     /locus_tag="IV329_001335"
     CDS             8512..9153
                     /locus_tag="IV329_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940525.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9150..10067
                     /locus_tag="IV329_001336"
     CDS             9150..10067
                     /locus_tag="IV329_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8940526.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10064..10735
                     /locus_tag="IV329_001337"
     CDS             10064..10735
                     /locus_tag="IV329_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940527.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11276..11764)
                     /locus_tag="IV329_001338"
     CDS             complement(11276..11764)
                     /locus_tag="IV329_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8940528.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11965..12231)
                     /locus_tag="IV329_001339"
     CDS             complement(11965..12231)
                     /locus_tag="IV329_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8940529.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12231..12494)
                     /locus_tag="IV329_001340"
     CDS             complement(12231..12494)
                     /locus_tag="IV329_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8940530.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12487..12759)
                     /locus_tag="IV329_001341"
     CDS             complement(12487..12759)
                     /locus_tag="IV329_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940531.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12970..13155
                     /locus_tag="IV329_001342"
     CDS             12970..13155
                     /locus_tag="IV329_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8940532.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13297..15120)
                     /locus_tag="IV329_001343"
     CDS             complement(13297..15120)
                     /locus_tag="IV329_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8940533.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4394 a         3249 c         2855 g         4667 t
ORIGIN      
        1 aagttattta ttattattta tatgacacac ccgattattc aatattgagc aatatatccc
       61 atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt ctgccctacc
      121 tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct cttccagcgt
      181 ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg tttcaacagt
      241 agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg ctgcacgatc
      301 gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc
      361 tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt tcatgctatc
      421 taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa aaaaccacat
      481 tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt tcacctcact
      541 aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta gttttttact
      601 taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga acccgtattc
      661 ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg
      721 tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt
      781 ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca
      841 atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag
      901 acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc
      961 aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat
     1021 caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat
     1081 ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc
     1141 gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc
     1201 aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga
     1261 cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc
     1321 aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga
     1381 taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt
     1441 ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc
     1501 ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag
     1561 ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg
     1621 ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt
     1681 tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa
     1741 acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga
     1801 caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta
     1861 ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat
     1921 tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa
     1981 aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg
     2041 gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct
     2101 tattatgatt cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc
     2161 agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat
     2221 cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca
     2281 aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg
     2341 cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt
     2401 agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga
     2461 tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa
     2521 attgatggat ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca
     2581 tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc
     2641 aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt
     2701 gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc
     2761 cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag
     2821 taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg
     2881 atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa
     2941 ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc
     3001 aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc
     3061 taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga
     3121 ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg
     3181 gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac
     3241 ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg
     3301 aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg
     3361 aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca
     3421 tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa
     3481 gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat
     3541 cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc
     3601 tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc
     3661 atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt
     3721 gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc
     3781 gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc
     3841 taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt
     3901 gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat
     3961 cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg
     4021 cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa
     4081 gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac
     4141 atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat
     4201 catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc
     4261 tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc
     4321 tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat
     4381 tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc
     4441 tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga
     4501 gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg
     4561 tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt
     4621 ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc
     4681 tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat
     4741 tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt
     4801 caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt
     4861 cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga
     4921 ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa
     4981 cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc
     5041 atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt
     5101 cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag
     5161 tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc
     5221 gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga
     5281 aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt
     5341 tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg
     5401 ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac
     5461 ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa
     5521 aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa agggcttatc
     5581 gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg
     5641 atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga
     5701 tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta
     5761 gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac
     5821 aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac
     5881 aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa
     5941 attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat
     6001 ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa
     6061 tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa
     6121 catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga
     6181 taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa
     6241 atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat
     6301 atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa
     6361 tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt
     6421 caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc
     6481 aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac
     6541 taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc
     6601 tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc
     6661 ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc
     6721 tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt
     6781 cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt
     6841 cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc
     6901 taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt
     6961 attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc
     7021 ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa
     7081 tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat
     7141 atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc
     7201 aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt
     7261 gtattttttc attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa
     7321 agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc
     7381 gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg
     7441 agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa
     7501 gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt
     7561 cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat
     7621 atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa
     7681 aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta
     7741 tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc
     7801 atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac
     7861 cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg
     7921 gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatccaa
     7981 tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga agagttgatt
     8041 ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga agttatgcta
     8101 aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa actgatgagg
     8161 gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa aggatttatc
     8221 gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga cattttgaat
     8281 aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca acgcatttta
     8341 aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg
     8401 actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga tcccgcaact
     8461 actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc tatgcagcag
     8521 ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct ttatatttca
     8581 ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt actgacacgt
     8641 tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac aatcccttct
     8701 cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc tgcgattatc
     8761 gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg gatgaaaaat
     8821 gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt
     8881 ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg tttagctgcc
     8941 gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg tttaggcgat
     9001 ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg tacgatccct
     9061 gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca ccggctgaca
     9121 ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg accatggttc
     9181 ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc aatacagatg
     9241 aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta gcaagtctag
     9301 ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac aatctggcta
     9361 ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca gcggctcgct
     9421 acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat ccgaaaaaag
     9481 cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct
     9541 atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa aaataccatc
     9601 tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt gtagatactt
     9661 cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat ggcttctcct
     9721 tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa
     9781 tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat ctagtcatgt
     9841 tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta gacaacaaga
     9901 tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat accatcagta
     9961 cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa ccatcagtag
    10021 tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc aattggataa
    10081 aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct atatctttgc
    10141 acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag cagttgtcgg
    10201 gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg tgattcgttt
    10261 ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa tcattggatt
    10321 aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct taccgatcat
    10381 caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg tcggcaaagg
    10441 catggggatg actgctagac aacgactatt catggtagag ttaccactct ctgtttctgt
    10501 tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg cgatcggtgc
    10561 attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg caacagaggg
    10621 cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca tcacggactg
    10681 gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac gctgatgtta
    10741 ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt
    10801 catttttaag gcaataatcc actctgattc ttccctcctt atgggtaaag agttgctttt
    10861 cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt tcatatctgc
    10921 atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca ttttcccatc
    10981 actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt tctttgaatt
    11041 gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc atcagtcctc
    11101 gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc ggtaacttcg
    11161 atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa catatctagc
    11221 cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac gattcttatg
    11281 gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga agagcacccc
    11341 cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa aagatatcga
    11401 tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc acgactttaa
    11461 cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta
    11521 aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac catgccaacg
    11581 aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt ttcaacatac
    11641 ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt tggcgataat
    11701 cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg attttccttg
    11761 tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct aaactcaaac
    11821 aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca ataaatccaa
    11881 gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt tgttaactat
    11941 ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat aaatttctac
    12001 aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca gcctgcgtga
    12061 gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca tatatggatc
    12121 ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt
    12181 tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca tcaaagagct
    12241 tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact
    12301 ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc
    12361 agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat ttcagtatga
    12421 ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct aggattcatc
    12481 gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta cttgattaag
    12541 tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct tttttacata
    12601 gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta ctcctttacg
    12661 actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag caaaggtcga
    12721 atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt
    12781 gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta acggttattc
    12841 aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct ctttcgtctc
    12901 ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt tgtgaatcac
    12961 caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga ggctgagatt
    13021 cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata
    13081 ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat caaaatcaaa
    13141 atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc atctttaatg
    13201 gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag cttcaattaa
    13261 gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat ggtagctata
    13321 ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca aacttgtttg
    13381 gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca acacagtagt
    13441 tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga taattggatt
    13501 ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga aatgcttttt
    13561 aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa catgcatcgc
    13621 aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta cccgtgtcac
    13681 attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg catcattttc
    13741 taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa aaatgaacat
    13801 aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga caaacaaaat
    13861 cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca
    13921 tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag caaatggaat
    13981 gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga aaataaacag
    14041 caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta gtcgatcccc
    14101 acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg caagaactgg
    14161 aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag caaattgaag
    14221 gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca aaacagtgac
    14281 ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca cgaagaagaa
    14341 acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc ttggcttctt
    14401 aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag aaccagaaga
    14461 aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg gaagtgcagt
    14521 agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga tcagtaacga
    14581 gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt ccctcagccc
    14641 acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga cttgatgacc
    14701 gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag atactgctac
    14761 tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat tctttccaag
    14821 attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga tgatttgacg
    14881 atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg gtagcgaata
    14941 ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg taccatatgc
    15001 aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg ttaatttgtg
    15061 ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta agtaatccaa
    15121 tttctaaact cctttacaca caattttact tcaagtttta tagtc
//
LOCUS       DACDKL010000058        14693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDKL010000058 DACDKL010000000
VERSION     DACDKL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.058"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(222..1100)
                     /locus_tag="IV329_001344"
     CDS             complement(222..1100)
                     /locus_tag="IV329_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8940534.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1139..2257)
                     /locus_tag="IV329_001345"
     CDS             complement(1139..2257)
                     /locus_tag="IV329_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8940535.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2254..4353)
                     /locus_tag="IV329_001346"
     CDS             complement(2254..4353)
                     /locus_tag="IV329_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8940536.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4404..5225)
                     /locus_tag="IV329_001347"
     CDS             complement(4404..5225)
                     /locus_tag="IV329_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8940537.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5222..6031)
                     /locus_tag="IV329_001348"
     CDS             complement(5222..6031)
                     /locus_tag="IV329_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8940538.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6052..6546)
                     /locus_tag="IV329_001349"
     CDS             complement(6052..6546)
                     /locus_tag="IV329_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8940539.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6558..7046)
                     /locus_tag="IV329_001350"
     CDS             complement(6558..7046)
                     /locus_tag="IV329_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8940540.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7123..9819)
                     /locus_tag="IV329_001351"
     CDS             complement(7123..9819)
                     /locus_tag="IV329_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8940541.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10247..11320)
                     /locus_tag="IV329_001352"
     CDS             complement(10247..11320)
                     /locus_tag="IV329_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8940542.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11317..12144)
                     /locus_tag="IV329_001353"
     CDS             complement(11317..12144)
                     /locus_tag="IV329_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940543.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12145..12951)
                     /locus_tag="IV329_001354"
     CDS             complement(12145..12951)
                     /locus_tag="IV329_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940544.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(12963..14048)
                     /locus_tag="IV329_001355"
     CDS             complement(12963..14048)
                     /locus_tag="IV329_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940545.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14065..14607)
                     /locus_tag="IV329_001356"
     CDS             complement(14065..14607)
                     /locus_tag="IV329_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8940546.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         4453 a         3262 c         2420 g         4558 t
ORIGIN      
        1 ttaagtgtac gccaccagtg aatatcctca aggtatacag cgcacaacaa aaagaaaaaa
       61 ggaaacgcag aagcatttcc tttcagtgat ttaatgaaaa caacacgatt tgacttagag
      121 cctttttatt tgaattcatt ttcgctttat ccaacttcat ttgatcattt gattactcgg
      181 ttaaatactt ttgacatgag tatcaatgaa acattcatta attatggttg cttatttctt
      241 accgcttttc tggccaatgc taatccgccg attattccgg cattatcacc taattcgggc
      301 gtaataatgt agctatcaat tggtggtatt tcaacatatc catttataag ctcaacaaat
      361 ttatcctgaa cttttttctt catgtgttct tgtttcatta caccaccgcc aaaaataatt
      421 ctatctggcg aaaacatcag tgttgtattg tatgcacatt gggcaaggta aaaggcttca
      481 atctgccaaa acgaatcgtc agccaataaa ttctggcctt ttacacccaa ccgtttttca
      541 atcgctggtc ctgctgccat cccctctaaa caatcttggt ggaagggaca gtttcctgaa
      601 aaattgtcct ttggatgtcg cttgatcaac atatgcccca tttccggatg actgaaccct
      661 tccacgaaag tcccattgaa caaggcacca cctccgatac ctgttcccac agtgaagtat
      721 gcaacacctg ctaacccttt gccagaacca gcaacatatt caccatagca agccgcattg
      781 acatctgttg tccattcaat cggaatattg aaatattgct ttagcgttcc tacaaaattg
      841 aaattttgcc aagcaagttt tggtgttgat gtgatatgac cgaaactcgt tgatttgcat
      901 tgaatatcaa ttggaccaaa gcagccgacc ccgatgcctg ccaattgttc cttaaaaggt
      961 ttaaaaaaat caatgacctg cttcattgtt tcaactggag ttgttgtggg aaaactgact
     1021 ctttccaaaa tcgttaattc ctcagtaccg actgcacaga caaatttagt ccctccagct
     1081 tcaattgccc catatatcat cataggttcc tcccttcgaa tatgtgaaaa acacttcatt
     1141 aacgcggcaa gcgttctcga tcagtgaaat gacgtttagt atttcctgaa gcttctgttg
     1201 aataccagta agcgacacta gcaacatcgt cttgccgctc aaataagcga acatcatcgt
     1261 tgccgatttg ttgtaaagtg accgataaat cttcggtaaa acagatgggg tcttgaagat
     1321 gccaacgata aagaccatgt cgtggcattg cgtcgtcacc aaaggcatga acggcattca
     1381 attttcccgt ttcaaaacga tctcttgtgg catctctatt tgattggtaa ggatacccta
     1441 aaaaggctgt tgaatagttt ttcgcatgtg gacgtccgaa ctcatcgtac tcatgaaagg
     1501 cccacgcccc gccaaaataa tcttctgaac ctgttgaggt tacggtcgga tactcactat
     1561 ccccatcaat gtagaattta aactcgcctt cgccccacca ataacgttct aggcaggtaa
     1621 gcgcaaggta agtacccacg taatagcctt gaccctttaa tcgatcaatt aacttataat
     1681 cctcctgtaa tttggtaatg cgttcccgat gccaatttgc gtgaaaatat aggctatttt
     1741 cttccaattc gtccaccaaa gcgtaattta tcgtataaaa aaatgaggac atatccttcg
     1801 gatgtaaatt ctcgatcgta attttagcgg atgtttggaa aggcatttcg aaataggaat
     1861 taaagccccc tgtaggatta accactatcg gcaacgagtt cacctcgcaa cgctctccaa
     1921 acccattaca aaagaaatcc cctaaaggac tttcaacagc agggctatcc gacccatccc
     1981 aataaattct taagattaaa tcacgtaaaa caaaactgcc aaattctgtt cgatcacgaa
     2041 tcgtcatcca catatgacga attattcccg ttccagtaat atccgcaatc acagtttctt
     2101 caccagaagt cagcggaatt gcgccacgac ctttgcggct tggtcctagc tcgcttggca
     2161 acatcgcagc tttacctttc tcgcctgttg gattttcggg agtgattgct cgactctgga
     2221 tttttttaaa ttgggtaata ttttttagta tactcatctt tttctccttt gaatgtgaat
     2281 cgtttttccc ttttctacgt gaagaatttt tttcgtatca cttattttca atggcttttc
     2341 tgacaaattt gaaatactca actcggattg cgtacattca atcgaaatga aggcttgacc
     2401 gaacttcaaa ttcttccaag aaaacgttaa attatcaaca ggatcaattt gcaatactcc
     2461 ttttttgaat ttcaatccga aatattgact gatcattagg ctgacaaaaa gacccgatgc
     2521 ccagccgcat ttgccgcagc caaagtctcg cacgacttct ccttgttgag cctctaagcg
     2581 atacggccac caccaccacg aaccgtctaa atctgccaaa caaattagtt cttctaatcg
     2641 ggttacgaaa gcttgagggt tgtgcagctc gctcattaaa acagtgataa agccaggaaa
     2701 agtggcgccg gattctaatc cccaagtgat gcctttgatt tcctcgctat aggttggatt
     2761 gaaagaacta gcagaaaagg ccatggtgtt ctgatataaa tgatctttat tatctaaaaa
     2821 ttgataaaaa ggcatcaacg tagtgtctga ttcttctcca tcatgcatca ataaattaat
     2881 ttttccatcc acaatcacat cacttaaaaa gattaaccct tgctcaagaa tcggtttttg
     2941 atagtgcttc actgaatagg ttgttttctc ctcatcaccg attccctcca aaaattgctc
     3001 accaaaagga ccatcgatag tcatttgttt acaaattgat tcctttagtt tacaggcaat
     3061 gtttttgtat cgcttgctaa gactcagttg ttctagggct tcaaaaattt ctgcaagtcc
     3121 aatacaggcc ttccaaagac aaagattgct gccggtatga tactttccaa gtgcaaaggc
     3181 gtctgataac caaacagatg gaaaaagcat tggttcccct gaccgacgtt gagctaatat
     3241 gtcctcaata attcttgaag cattcagtag aacctgcgga caccgctcaa agaattttgt
     3301 atccccagta tgttcaaaat aaagactagc cagtaacgca ctggataacg aattactcag
     3361 agaatgattg atacctcctg taaattttga accaggaaac ttaacgccat attgatcaaa
     3421 ccatagaacc gttttttgac ataactccgg ttcaaacatg gtgaatggca atgatgcgta
     3481 atacatatcc ttattccaaa ttcgattagt agcaggatag ctgccccaat tgctgccgac
     3541 gacctgtccc tgtcgattca taccgaaact agaaaagctt tgataaagtg cacgacgata
     3601 gagatggaca actctatcgt ttggcattga cagctgccca aatagctgag caaaatagtt
     3661 caatgtttcc ttcagccatt tttcgatatc agattcctga aaagtcatta cttcttcaaa
     3721 agcatttggg tccacaaaag caatggcgaa gcttgccact tcctgttgtg cgatcgtgtc
     3781 cttatttcct ttttgagaaa tcaacacatt ttgctgatcc gaatacttct cttgatacga
     3841 aggcacatct ttcactgaaa cttgaaggct ttcgtgtgtt tcattcacaa tgtaaacctg
     3901 ttgaacaagc atacttaaac gcttattagc cagtatggga gcaaaggata ctacaatgac
     3961 cttaaacgtc gaataatgat gaatatatct aggcaaacag tcttgaatca catcgacttc
     4021 aatacgatga tccgattctg acaaatggta gacaatcccg tttacttcaa cctttaagga
     4081 taacgaattg gtttgagaga gctgtttatt gacccaaaca cctggttttt cctcagttag
     4141 taaattttct cgataaaagg tcaacgtttg aatcgtcccg caaacatcaa ggtttgctaa
     4201 tgtcttttga ttacttacat cgaaggtcaa tccctttaca tcaggaatat tccgatagta
     4261 gctgtattcg tttgaagtag tcacatttcc gtcgtttaac tcagcattaa aaaatgggtg
     4321 tttgggtgaa taaattactt cattaatagt catcttttcc ctccttgcta aaaaaactgc
     4381 agcctagtag ctgcagcttc ctactatgcg attccaagta atgacaataa aattcccaag
     4441 acgaccattc caaaaataat tttgatgacg ccgacttttt tatctagtaa tttgaaacag
     4501 ctcaatgtta ccaataacgg tacaattcct ttaaaaatac tatcgaggat ttcttggaca
     4561 ttcattagtt cttcgccctt cattgaaaag ctcaaaacgg tattgaattt gaccatactg
     4621 atcgtcattg cacctaccat gatcagaccc aagatactag cagcttttgt cagaatttca
     4681 attaacccgt tcgcatacag cttttcaata tatttagagc ctaatgaata gcctaaaacg
     4741 ccgccatagt atcgcatcaa gtaattaggt atattgaaca taactaaaaa gataattggt
     4801 gccaaaatat ttccggcatt ggctaagttg atcgctagtc ccgcggtaac gacacgccaa
     4861 actccccaga acaatgaatc gccaattcca gccaatggtc ccatcaaact cgttttgatc
     4921 gcgttgatac tttccacgtc aaaaccagct ttttcagaat tttccttctc catcgatgcc
     4981 gcaatcccca tgacaaagga cgcgcaagga atggtcgtcg aaaaatactg tgtatgacgt
     5041 tccagcgccg cagctcgatc ttcttcactt ttgtagaaat gattaatgaa gggcatcata
     5101 gaataggcga aaccaccgcc gcccatcgtc accggattca ccgaagcata catagtcatc
     5161 gagcgccaaa acatcttccg aaccaatttt ttctcatctt tagaaattcc atcagctaat
     5221 ttcattgaaa gaaatcctcc tcttcttggt caagcgttgc atttacagca gcatcgcttt
     5281 gaactgcatg aacagatata ttttttagtt gtagatctct ttgggctata acaattgcaa
     5341 taatcgttcc aactgcggca attgcaatca atggtaactc gagatacgcc ataacaacgg
     5401 ccccaaagaa aaaccacaca gcaatatttt tactccaaag catccgtaac agcatcgcca
     5461 tcccaactgc cggcagcaga tttccagcga ctgttaatcc gttcatgatg acctctggaa
     5521 tattatccaa gacgcttttg acaacatctg ctccaaataa aacggcaaag aatggaataa
     5581 gagcataaag cgcatagtgg acaaaccaca taaagaagtg cagattgatg attccttttt
     5641 gatcaccaga ttttgcaaat ttgaaaaatt tagcagccat cggtccacat acaaaagaaa
     5701 aaatcatatt cattaactgt aatcctaata ctgctagtgg aaagacaatg ggaataatcg
     5761 catccacgtt tcctcccatt ttgattgcga acgctgtagc cagcccagca gccaaagaag
     5821 gctccgccgt actagagatt ccgatattga cgacacccat aaagatggct tctagtgacg
     5881 ccccgacaat caaccccgtc tgcaaatccc ccatcaacgc tcctgctaac ggagcaacaa
     5941 caatcggacg actcagcatg gacatcccga acatttcatg tccagcaatt ccaataaaaa
     6001 cggccaaagc gactacaaat gcatcataca taatttttct ccttcctttt tctaaaaatc
     6061 actcaaaata gagatagctg aacgtttttc catcgccggt gtaggctgaa aaacggtatc
     6121 tggataaagt tcaactaatt cttttaagtc ttgaatttct gacgtcgtta acatcacttc
     6181 ctttgataag gttttcttat cttccgatgg cggtgtcatt ttcccagcat tccccacgtt
     6241 cattaattcg atttccgttg ggaaagcttt cgcaactttt accgcatctt tgacattagg
     6301 gactaaaacc atcaagcgca tgtccttcgc ccgcggatct tttaacaaat gaatcgcgtc
     6361 atcaactgtt ttaataatga ctttgacatt cgatggtgca gccatcttca atgtcatctt
     6421 ttgaatatcg ttggtagccg cctcatcgct tgctactact aacccggtta aatttagact
     6481 ctttgtccaa gtcatcgcta cttgtccatg aattaatcgt tcatcaattc gcactgcttt
     6541 aatcattttt cttcctccta aaaaaaatct tcctcttcat ttactacatg tacctcatca
     6601 agaatggtca tctttaattc ctgttggcat ttatttacca gttcctgaat atcctccttt
     6661 gttaattcct tggttgccaa tactatttcg agcagaattg gcagattaaa gccagacaaa
     6721 atagttacag caatctcttt ctccaaaaag gttttggtaa tcttctgatt gacacttccg
     6781 ccaaataaat cggtaaaaac aaaaatctga tcctgctcgt tcacctggtt tacaaataca
     6841 ttcaactcct gttcaaaatc acactcatcc acataagcat taatcaccgt aatctcttga
     6901 accttccccg tcagtaattg aatactgcta agaaagcctg aagctaagtg accgtgagtc
     6961 gctattagaa ttttcttcat atggttaatt cctttctttc tgttcacttt tatataagca
     7021 attcctgtgc caacttttaa aaacattggt ttaatcacgt taattcacat taaaaaaagt
     7081 gtctaaccta aaaaaaacag attagacact tttttagaca ctttattttt gaatatacgg
     7141 tcctaataat tcatagagca gcgaaagctc ataagcattg actttaaaat tatatttcat
     7201 ctccattgat tgaaaaatac ttctagaaac ataataaaat tcatcttcat ctggtttcaa
     7261 atcataattc ggttcttcag atttttctgt ccgagccaag aataaacgtt ccatcatcaa
     7321 tgccgtatgc atatagagat ttagttttac acggccattt aaagtgaaat gatagtaatt
     7381 ttcatattta gaaatgactg tttccacttc actcatcacg atcgttgggt tcagaaagtt
     7441 caaacggtcc gcaattcctt ccaacgaaaa gaattttact agctcctgaa tcaagcggtt
     7501 aaattctgcc ggcttcattt caggactcaa aatatgctcc aaattggctg ctccatcacc
     7561 gtctaagatg tcataaacat taatattagg aattgaaaag gaacgtggaa gatccgaagt
     7621 cgtgatgacc aattttgtct tgtcaaaata agtttcatca ttttggtcga ttaattcacg
     7681 tagttctctg tagtccttcg tgaaaacttc gatggattca tccgataaat gatgacgaaa
     7741 aacatctttc aatttgtccg aaatccccaa acccgacata caggaaatga taatgttgct
     7801 actttgagaa aagccttcaa agtattgaac attgattgaa tacttgtctg ctgcatcctc
     7861 cgcaatctgt ttaaatggcg cattcatcgt catcttcaag ccaatatcaa gtgcaatgga
     7921 agtcgtcaaa ttattcagaa gaagtaactc cccttttaaa tcgttcttga tctgtgtgta
     7981 aatttgattt aaagagccca tatctacgat cagcactaga ctatctgtat tcacttgccg
     8041 tttcacgaaa ttccttgtcg attcgacaat ctctgacaca ttactctcta ctggcatatc
     8101 caacgcttca aaaacataat tgccgcataa ttgattcact actgcttgaa tgctgctagc
     8161 cgtactatct ccgtgagcaa gcaacaggcc cttcagctcg atggattcat ctacataatc
     8221 agataaagtc acgacaataa gcagtcttag aaagttctcg tccttttctg agagcggtgg
     8281 taaatgctcc aagaactgat tacataagta agtcgtccga ggtagatttg cagctaatgt
     8341 acgatttatc ccggttaagt ctgtcttata gcttggggaa atgacatagg atctgctaat
     8401 aatttctgca acaggtccac tcgtcgtcaa tccgtacgtt tttcctaaaa cggataccca
     8461 gacctttcta aacatctgaa tcatcaattt tctactcatt gataaaaaag aatccttatc
     8521 aatgtgatgc accaaatgct gtacttcatt ctttatcgaa gaaaaatttt cattgaaatt
     8581 accttcattt atcagctgag taattttttc caaaatcgaa tcataatcaa acggtttgat
     8641 tcgatccgtt tttatctttt gatttttctt tatcaataaa ctagcaatcg gcaagctttc
     8701 tacttcggat tccagcatat ttaactgaat caagatctca tttttcccaa tcgttaatgg
     8761 ttcttcttgt gttcgactat acacatccgc acaactgatt ttaatgatat tcttcagctt
     8821 accaatattc ccactaaagg gatgagataa cattaaagac aaggcttctc ttgaaacaat
     8881 aatatctttt tgtaaaacat ccgcttcatt ttgaaaaaat aattgaatca gctctaatcg
     8941 ttctcgaatg gggcgatctt caaaagttgg caacataaca ctaatttgaa tacgccgatc
     9001 aaaggtgtct aataaataat tttcaccttt ttccgtcgtg gcgaaaataa aacgaacctt
     9061 agcagaatgc caatttacat tttcaccgac tggacgatag tttccagtat caataaatag
     9121 aaagagcttt tcttgatttt ctttcgatag gcgatggacc tcatcgagaa aaagatagcc
     9181 gttgttggcc gttgccaata aaccatcggt atccttttca gcccccgtaa aggccccctt
     9241 cttgtatcca aacagtgttg ccgaaagcag ttcgggattg tccgcataat cggcacagtt
     9301 taagatagca aatggtgcat ctttttcaat aatttgctca tggatagcgt attcaaaaat
     9361 ttttgtggct aaaaaacttt ttccaacacc cgtggctcca tttattaata cactgagtcc
     9421 gttaggcgga tatttgacag cggctataca tttttgaatg acctctctca gacttccgtc
     9481 atgaccaact acagaatgaa aggcttcgtt tatttgctcc gtattatcta caaccgcata
     9541 ttcatctaaa ggcttccaca aaacaggctt tcccggtaat ttctcgacct ttttatcttc
     9601 cactaatctc gtcaaataat ggctggcatt ctgacgagag atacctagat tcttcgaaat
     9661 tttctcggca gtgacagaag acatattccc tgttagtcgt atcagctcat caaaaactcg
     9721 atctttagcg ctctttttca ttttttcctc cagtttaatg tgtctaaaag cataataaac
     9781 tatcggctgc aatatgtcta ttctatattt gtattccaat ttttactttg aaacacaaat
     9841 agtcgaatat ttgcctcaga tatctgacaa aataatttat ctaccaattt agtttttatc
     9901 taatatgatt tttatgtttt ttcatagcta caaatattga actggattca tatattagtt
     9961 ttattaatag attacagaag ggttaagtat atttgcatag tttccaatta ctttatcctt
    10021 ctgtaacttt ttcaatctta gatttactag cccgtctcgg gccctatttt acaatggttt
    10081 tgtccacttt tctctggtag aaaaacgtgg tagaaaaaat aaaataacac aaaaaaagcg
    10141 gcgtttcatc cgcttttggt agaagattgg aaaactcaga tagaaaaagg acgattttta
    10201 gctctttaga acgttgataa gtccccattc ttactgagtt ttgccattat ctcctataca
    10261 tcttgaattc taagaatagg tcattataga tgcctaggta tttcgatcct agatctttgt
    10321 agacttcaag atgggagatc gtgtcatcag atggatagaa ttgttcgtcc gacgaaatgt
    10381 cttttggcaa taacgctttt gcttcttcat ttggtgtaga ataaccaata tactctgcat
    10441 tttgtgccgc attttccggt tctagcataa agttgatgaa atcataggca ccttctttat
    10501 ttttcacggt ttttggcata acgatattat caaaccaaag attggatcct tctggtggga
    10561 tcacataatg taaatgttca ttttcccaca tcatatcggc agcttcgcca gaaaatgtca
    10621 ctgccacgga ggcttcttca ttgatcatat acattttgat ctcatctgcc acgattgctt
    10681 tgacatttgg tgttaaatga ttcaatttat ttgctgcttc tgtcaactgt gccatattct
    10741 tactattcaa agaatagcct aaactattca aagaaagccc cataacttct cgtgcaccgt
    10801 caatcagcat caaactgtct tttaattccg gcttccacaa atcatcccaa tgcttaattt
    10861 gatttgcatc aaagaattta tcgttatata cgatccctaa agttccccaa aaatatggaa
    10921 tcgaatactg attatctgga tcaaaatcca agtctaaaaa tcgtggatca atattattta
    10981 atcctttgat cttcgaatga tctaatggaa gtactagttt ttctttgatc attttttgga
    11041 tcatatattc acttggtata gcgatgtcgt agtttgtccc accttgctgg atttttgtga
    11101 acatcgcttc attagaatca aatgtttcat aattcacttt gtatccatat tctttttcaa
    11161 atttcgtgat caaactagga tcgatataat ctccccagtt atagatggta agtacctttg
    11221 ctccactcat gccacttgtt ttttcaagtt gacgaacacc aagcagcagc accgcaataa
    11281 tgatgagaat acctaaaaac agagattgta attttttcat cgcagccgtg ccaccccctc
    11341 gcgtactctt ctgtttttct tcagccgttt tggacggttt tctttactaa taaagtagta
    11401 tccaatgacc aatatcatgg agaacaagaa aactaacgca cttagcgcat tgatttctaa
    11461 actgattcct tgtcttgcac gagaatagat ctctactgag agcgtcgtaa agccatttcc
    11521 tgtgacgaaa aatgtaaccg caaaatcatc aagagaatag gtaaatgcca taaaatatcc
    11581 agctatgatt cctggagaaa gataaggcaa aatgatattt ttgactactt ggaaattatt
    11641 agcaccaagg tctctagccg catcgaccat tgaatcgttc atttcctgca attttggcaa
    11701 gaccatcaag acgacgattg gaatgctgaa agcaatatgg gaaagcaata cactctgaaa
    11761 acctaatccg aatcctagta gtgtaaagaa aatcaagaaa ctagcaccga taataacatc
    11821 aggagagact aataagatgt tattcatgct caacaacgct gtccgtgcac gtcgtttttt
    11881 cgtgtaatag attcccatag ccccaaatgt cccgatgatc gtggcgatca atgcagataa
    11941 gaaagccagc atgaacgtat tcaatacgat aatgatcaaa cgtgtatctt caaaaacagc
    12001 ttcataattt tcccaagtaa atcctgtaaa gctgttcatc gttcctcctt cattgaagga
    12061 ataaaaaatc agatagaaga tcggtgcata taagaataaa aagacgatcc ctaaataaag
    12121 atacgaccat ttaaattttt tcatttatga gcgacctcct tttttcttct cgcctgtcag
    12181 gagcatcacg atgaacatag caatgattaa aatcacaccg attgtagaac ccatccccca
    12241 gttttgtgta accaagaaat gctgttcgat agccgttcct aaagtgatca cccgatttcc
    12301 gccaatcaga cgagtcagca tgaataaaga aagagaagga ataaatactg cttggacacc
    12361 acttttcaca ccatttaatg ataacggaaa aatcactcgt cggaaggtct caaagctatt
    12421 cgcgcctaaa tctctactag cactgatcaa agaaggattc atctcttcca atgcattgaa
    12481 aattggcata atcataaatg gaatctcgat atacgttgcc acgaacagaa aactgaagtc
    12541 tgtgaataat atctgtttcg gcccgatccc gataaaatca aagaaatgat tcacgctgcc
    12601 gtgcacactg aaaatcccaa taaatgcgta agctttaagc agcagattca cccatgtagg
    12661 caaaataatc agcatcagcc ataattgctt gtgcttcagt tttgttaaaa aataagcagt
    12721 cgggtaactg atgatcagcg tgaaaaacgt gatcaaaaat gcataccata cagaattgat
    12781 cgtcatagaa agcgatgtcc ccgacgtaaa ataagataga taattattca gcgtgaattg
    12841 cccattcata tcaaaaaaag attgataaat gatcattaat acaggcgcga tcacgaataa
    12901 accgagccac aaaatataag ggacggaata gatcctgcgc attgttttca ttcttgtccc
    12961 ccctattctt cgtagctgtc aagacgtgca tcgaattctt cttcagactc gttgaaacgc
    13021 atgacatgga tatcttctgg ctcaaaggcc aatcctactt tgcttccttc tttagctttt
    13081 ttcgttgaat ggaccatcca ttcattttgc tgttcatcat aacagatgat ttcataatgg
    13141 acaccacgaa acagctgagt atcgaccgtt acgactagtt ttcctttatc tggtgtcgtg
    13201 atcgtcaaat cctctgggcg taaaacaact tcaactggtt catttagacg catcccgcca
    13261 tcgacacatt caaattgctt accaacaaac tcaactagat tatcttcgat cattacaccg
    13321 tccacgatat tgctctcccc aacaaaatca gcaacgaaat gattgattgg ctcatcataa
    13381 atatctacgg gtgtaccact ttggacgatt ttccctttat tcatgacaaa aatctcatca
    13441 ctcattgcca acgcttcttc ttggtcatga gtaacaaaaa taaacgtgat tcctaatcgt
    13501 tgttgcagat cccgcagttc atactgcata tctgtacgta gtttcaaatc aagcgccgat
    13561 aacggttcat ctaatagcaa tactttcggt tcatttacaa ttgctcgtgc aatcgctaca
    13621 cgctgtcttt gaccaccaga catttcacta atctcccgtt tttcgtatcc aggaagctgg
    13681 accatacgca aagcatcttt taccttcttt tcgatatctg atttggacat tttcttgatc
    13741 ttcaatccaa aagcaacgtt atcaaaaaca ttcatatgag gaaaaagtgc ataatcttgg
    13801 aagactgtat ttacttgtcg cttattagct ggcaaatcat tcaaccgttt cccttcaaaa
    13861 tagatgtccc cttctgtagc ttcggtgaat ccagctataa tacgtaggat cgttgtttta
    13921 ccacagccgg aaggaccaag cagtgtataa aattttcctt gttcgatctc aaaacttact
    13981 ttttttaata ctgtttcatc atcatagcgt ttcactactt gatcaaatga aatgattggt
    14041 ttccccacgt ttttaccccc tatattacaa atatgattgc gttgcaacaa tcaatacttt
    14101 tgttcgtttt ttgagatgat tccttaattg atgttgtttt gtcgcttgat aatagatcga
    14161 ctgtcctgct tttgccacat agaccgtctc tcctaagctt aactctaatt ccccttctaa
    14221 tacaaaaata aacgtttcag ataatgaagg ttcaaacgtc ttatattctc ctgcaccttc
    14281 aaaagtcagc aagacaggct ccatttcttt ttcattcgaa tcagccacga gccattccaa
    14341 ttcatacccg ttttcttcat cgaaataaac cgtatgatct tcttttgaat aaaccacttg
    14401 cagattcgct gcttcttgat gaaaaaattc ttctggagtc acacctaaga cttcaagaat
    14461 agaaaagaat gtttccattg atggtgaact taaatctcgt tccaactggg aaatataccc
    14521 ttttgacaaa tctgtgcgtt cgcctagttc ttcttgtgtc aaattctttt ggatgcgcaa
    14581 atttcttaac ttctcaccaa tttccataat ttcctccgaa tcgaagtttt ctaatagtaa
    14641 acttcctgtt tactaccact cgtattagta tacgaaattt attgaaaaag gca
//
LOCUS       DACDKL010000059        14393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDKL010000059 DACDKL010000000
VERSION     DACDKL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.059"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..553
                     /gene="hslV"
                     /locus_tag="IV329_001357"
     CDS             5..553
                     /gene="hslV"
                     /locus_tag="IV329_001357"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8940547.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            567..1967
                     /gene="hslU"
                     /locus_tag="IV329_001358"
     CDS             567..1967
                     /gene="hslU"
                     /locus_tag="IV329_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8940548.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1985..2776
                     /gene="codY"
                     /locus_tag="IV329_001359"
     CDS             1985..2776
                     /gene="codY"
                     /locus_tag="IV329_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8940549.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2858..3733
                     /locus_tag="IV329_001360"
     CDS             2858..3733
                     /locus_tag="IV329_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8940550.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="IV329_001361"
     CDS             complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="IV329_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8940551.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4630..5064
                     /locus_tag="IV329_001362"
     CDS             4630..5064
                     /locus_tag="IV329_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8940552.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5230..7275
                     /gene="parE"
                     /locus_tag="IV329_001363"
     CDS             5230..7275
                     /gene="parE"
                     /locus_tag="IV329_001363"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8940553.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7287..9737
                     /gene="parC"
                     /locus_tag="IV329_001364"
     CDS             7287..9737
                     /gene="parC"
                     /locus_tag="IV329_001364"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8940554.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10014..12245
                     /gene="pflB"
                     /locus_tag="IV329_001365"
     CDS             10014..12245
                     /gene="pflB"
                     /locus_tag="IV329_001365"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8940555.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12489..13250
                     /gene="pflA"
                     /locus_tag="IV329_001366"
     CDS             12489..13250
                     /gene="pflA"
                     /locus_tag="IV329_001366"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8940556.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13335..14261
                     /locus_tag="IV329_001367"
     CDS             13335..14261
                     /locus_tag="IV329_001367"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8940557.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         5050 a         2494 c         3124 g         3725 t
ORIGIN      
        1 aaacatggtc gaatcaccat ttcattcaac cactatctgt gccgtcgaaa aagacgggaa
       61 atttgctatg gctggagatg gccaagtaac aatgggtgaa tctgttgtca tgaaaggcac
      121 tgcaaagaaa gtacgacgta tctacaatgg agaagttgtt gtaggtttcg ccggaagtgt
      181 tgcagatgcg tttactttag aagaaaaatt tgaaggcaaa ctaaacgaat ataacgggaa
      241 tctacaacgc gcagctgtcg aattagctca agaatggcgg acacagcaat ctatgcaaaa
      301 acttgaagcg atgctgatcg taatgaatga taaagaaatg cttcttgttt caggtacagg
      361 agaagttatc acaccagatg atggtatctt agctatcgga tcaggaggga attttgcgtt
      421 ggctgcagcc cgagcaatga aaaactatgc tcaaaacgac atgagtgcga aagaaattgc
      481 agaaaatgcg ttaaatattg ccgcagatat ctgtatcttt actaatcata atattatcgt
      541 agaagaactg taagaggtga gtagatatgt atgaattaaa acaaacacca agacaaatcg
      601 taagtgaatt agatgaatat atcgtaggac agcacgcagc aaaaaaatct gttgccgttg
      661 ctttgcgtaa ccgctatcgc cgtttgcagt tagatgaaaa aatgcaacaa gatattacac
      721 caaaaaatat gctgatgatc ggacctacag gggtcggtaa aacagaaatc gcacgacgtt
      781 tggcaaaaat cgtcaatgca ccatttgtca aagtcgaagc aacaaaattt actgaagtcg
      841 gttatgtcgg aagagatgta gaatcaatgg ttcgggattt agtagaaaat gcgatccaaa
      901 tcgtagaaaa agagcagtac agccgtgtat acacaagtgc taaaaaaaat gcagaaaaac
      961 gtttggtaaa aatcttggta cctggtatca aaaaagaaca gaaacaatcc actaatcaat
     1021 ttgaacaaat gatgaatctt tttaatgcta gtcagcaaaa taccgctgaa ccgcaagaag
     1081 aagtgacaga agatattcga atcaatcgcc gtacagtctt ggaacaacta caaaagggtt
     1141 tactggatag tcgtgaagta accattgaag ttgaagacca aaagaaacct gcaccaatga
     1201 tgaacaatgg attggagcaa atggggatcg acctaaatga aactctaggt gctttgacac
     1261 cgaagaaaaa agtggaacgg acggtaacag tcaaagaagc tttagagtta ttgatcaagg
     1321 aagaatcagc aaaattagtc aaggatgctg atatccatag tgaggcaatc aaattagcgg
     1381 aagaaagtgg aattattttt atcgatgaaa tcgataagat cacttctaaa tcacagcaat
     1441 ctggagaagt atccagagaa ggcgtccaaa gagacatatt gccaattgtc gaaggttcac
     1501 aagtaaacac aaaatacggg atcatccaaa cagatcacgt cctattcatt gcttcaggag
     1561 ctttccattt gtctaaacca agtgatctaa ttccagaact tcaaggacgc ttcccgatac
     1621 gggtagaatt agatgatttg acagctgaag attttgtgaa gattctgacc gaaccaaata
     1681 atgctttagt aaaacaatac gtcgcgttga tcggtacaga aaatgtgtca gttacgttta
     1741 caaaagaagc aattgaacgg atcgctcaaa tcgctttcga tgtcaaccgt gacacagata
     1801 atatcggcgc acgcagactg cataccattt tggaaaaact actagaagat ctattatttg
     1861 aagcaccaga tatgcaaatg ggagaaataa cgatcacaga agcatatgtg gatgacaaac
     1921 ttggctcaat tgcggcaaat gaagatttaa gtcgctatat tttataaaaa atcggaggac
     1981 gacaatgaca gacttgctaa cgaaaacccg caagataaac acacttttgc agcaaaagaa
     2041 tacttttgac atgcaagcag atttgcctta cgataaaatg gctgtcacac taggtggtat
     2101 tcttgacagc aacacctata tcataggaag tgacggagta ttactaggtt ataatgaacg
     2161 tcacgatgtg aacaatgaac gaatcaaaac aatgtttgtt gagaaacaat ttcctgaatc
     2221 ttatactgat acagtggcac agttgatgaa aactgaagct aatatcccaa ttactagtga
     2281 tattactgca tttccggtag agtcaagaga gaaatatcca tttggtctga cgactgtcgt
     2341 tccgatcttt ggagctggcg aacgtttggg tacgattatc ttatctcgta tggaacaatc
     2401 gtttgcagat gacgatctag tcttagcaga atatggagct acggttgtcg gtatgcagat
     2461 cctttatcag aagtctcgaa gcatcgaagc agatgtacgg agtgcaacgg ctgtccaaat
     2521 ggcaatcaac acattatctt atagcgaatt gaaggctgta caagctatct ttgaagcatt
     2581 ggatggagat gaaggtcgtc tgactgcttc taatattgcc gatagtatcg gcatcactcg
     2641 ttctgtgatc gtcaatgctt tacgaaaatt ggaatcagct ggaatcattg aatctcgttc
     2701 gttaggcatg aagggaactt atttaaaagt attgaattcc cgttttaaag acgagttagc
     2761 aaaacacagt tattagaagt aaggggactg gggcagatag aaatgtttgt tccagccttc
     2821 ccattttaaa aagtaagtcg aagttggagg aatggcaatg actgttatta ttcaaaatga
     2881 ccagctgatc gccgaaatcg ctgaacacgg tgcggaattg atcagtctga aatcaaaaga
     2941 acatggttta gagtatatct ggcaaggtga tccagcgtat tggggcagac atgcaccggt
     3001 tttattccct tttgttggac gtttaaaaga tgatcagtat gtttatcaag ggaaaacata
     3061 tcaaatgggg caacacggat ttgctcggga tatggatttt gaagtcattg aacatggaca
     3121 agaaatggct agtttcatct taaggagtac gccggagaca ttagaaaagt atccatttga
     3181 ttttgaatta gtgatcagct atgaattagg aggagacggc atcacggttc attatcaagt
     3241 ggcaaatacc ggtgaaaaag aaatgttctt ttctatcggt ggacatcctg ctttcaatgt
     3301 acctcttgaa gaaggattgt catttgaaga tttctatctg gcattttctc cattgaaatc
     3361 aagattacgc ctaccattga aaggcccatt catcgatatg gatcaaaaga cgttaggtca
     3421 gacaaatacg aatctggctt tgaatcgtga actttttgag caagatgcct tggtttttga
     3481 aacaaggggt ctaaatgcgt acgccattcg aagtgaaaaa tcttcccata gcgtaacttt
     3541 aagctataaa gatatgcctt atgtagggat ctggtctcct tatccaaaag aagcgccttt
     3601 cgtttgtatc gaaccgtggt gcggaatcgc tgacacggtg aacagtagtg gaaacttgat
     3661 ggaaaaagta gggatcaatc aactaggtgc ccatgaaatc ttcaagacaa aatattccat
     3721 cacagtgaag taagaggttg tgaccgagaa gttagcaggg aacaccgttt accaaggtgt
     3781 gtaaaaaagc tatcctgtaa gtaataaggg agtgtgacat cgatttgtca cactcccgtt
     3841 ttatctcttt ggaatgaaaa agtatcagga ttttttcttg ctgctattca aaccaaacgg
     3901 gattcgactt tcagtatggt ttctgatacg cttgatattg tctttatgcc gataaaagat
     3961 aaaggaagaa actaaaaagg caatgatcgt cagtaaccaa ttgggcttag ccaaaatcac
     4021 aggagctaca taaggaagga cgatcgtaga taaagtgatc aatactgcac tgatcatact
     4081 gctcaaactc accatacttg tacaaaataa actaacgata aagatgatac aagaataaac
     4141 aaaaaataca ggattaaatc ctaaaagcat tcctgcactt gtcgcaacag cttttcctcc
     4201 tttgaaccca gcaaaaatcg gaaatgtatg gccaagaatg gcgcataccc caaaccaaag
     4261 agggttaatc ccagaaaggt ggaagatcat cggcaaacaa gtagccaatg taccttttaa
     4321 aatatccata agcaggacaa caatcccggc tgttttgcct aatacacgaa atgtattggt
     4381 cgttccagta ttaccgctcc cgtgttgtcg gatatctttt ttgaaaaaca atttgccaat
     4441 ccagacaccg gaaggaatag aacctaaaag atatgctaaa ataagtaata agatcaattt
     4501 cattttttac cctctttcaa gttgaaaact atcctttatt ttagcatgaa taggtaacag
     4561 gaacaattga aaaaatgaaa gatacaagta taatagatgg aataaaaaga aaagcaaggg
     4621 gtggcagaaa tgtctgtaaa aaatccaaca caagaaacga ttttcaacta tttggaagaa
     4681 gcaaaaaata ttgccgtcgt aggcttgagc aataaaccag aacgaacaag ttacatggtt
     4741 gcacaggtat tgcaagacaa tggatataaa atcataccag taaatccatt attggctggt
     4801 gaagaaattc taggcgagaa agtctatgcg aatttgacag aaatcgatga tccaatcgat
     4861 atcgtagatg tatttagacg gagtgaattt ttacctgaaa tagcaggtga ttttttgaaa
     4921 actgatgcta aagtgttctg ggcacaatta gggattgaaa acaaagaagc agctgagatg
     4981 ctaagaaaag caggacgtac agatatcgta atggatcgtt gtattaagat tgaacttggt
     5041 aagttgaaag aaaaccaaaa ataaaaatga ccacaaagaa gagatgatcg atagagggga
     5101 atgtcgaaaa agcggttagg agataattaa aattgatttc tcgtctaccc tctttttttt
     5161 cacgaccttt tctagtatga taatcgtggt gcggttttgc cgcaaagtat tattgaagga
     5221 gcgtttgcct tggcaaaaaa agtaaacaac gaatacaatg actcgtccat ccaagtattg
     5281 gaaggattag aggcagttag aaaaagacca gggatgtaca ttggatccac tgacagccga
     5341 ggcttgcatc atttagtcta tgaaatcgtc gataatgcag ttgacgaagc attatcaggt
     5401 tatggcagtg agattgatgt gaccatccat gaagacaata gtataactgt tgcagatagc
     5461 ggacgtggga tgcctgtagg aatgcatgct tccggaatac caacagttga agtgattttt
     5521 actgtgctgc atgctggcgg aaagtttggt caaggagggt acaaaacttc tggtggactg
     5581 cacggtgtag gagccagtgt ggtaaatgcc ttatcgaaat ggctaattgt aacgatcgtt
     5641 agagatggcg tagagtatca gcagaaattt aaaaacggcg gtaaacctga cggtactttg
     5701 aaaaagatag gaaaaacaaa aaaagcaaac gggacgactg tccatttttt accagatgat
     5761 acaatattca gtacaacgaa attttcatat gaaattttag ccgaacggtt aagagaatct
     5821 gcctttcttc taaaaggcgt taaaatctct ctttctgatc ttcgtggaga agaaccagtc
     5881 aaggaaattt ttcattacga agaaggaatc aaagaatttg ttgattatct gaatgaagaa
     5941 aaagatacat tgacaccagt tgtttatttt tcaggagaaa aagaaggtat cgaagtagag
     6001 gtcgcttatc aatacaatga cggttattca gaaaatgttt tatcttttgt caataacgtt
     6061 cggacaaaag atgggggaac ccatgaagct ggtatgaaag cggcgatgac gaaatcttat
     6121 aatgaatatg cgagaaaagt tggtttgctg aaagaacggg acaagaatct ggaaggtagt
     6181 gatttcagag aagggttagc tgctgtactt tctatccgtg tacctgaaaa tcttctgcaa
     6241 tttgaagggc aaacaaaaga aaaacttggt acgccagttg ctcgtaccgt agtagataac
     6301 gtcatcagtg aacaaatggg cttttatttg caggaaaata gcgaaatgag ccagatgttg
     6361 gtccgtaaag caatcaaagc cagagaagcg agagaagcag cacgtaaagc cagagaagaa
     6421 agccgaaatg ggaagaagcg taaaaaaggc gaatctcttc tttcaggaaa attgacaccc
     6481 gcacaatccc gaaaccctaa gaaaaatgaa ttgtatttag tcgaagggga ttctgctggc
     6541 ggatctgcta aacaaggaag agacagaaag ttccaagcaa ttctaccttt acgaggaaaa
     6601 gtcatcaata ccgaaaaagc taaaatgcaa gatatcttga aaaatgaaga aatcaatacg
     6661 atgatctata cgattggtgc aggagtcggt cctgagtttt ctatcgaaga ctgtaattac
     6721 gacaaagtaa tcatcatgac cgatgcggat acagacggcg cccatattca agtattactg
     6781 cttactttct tttatcgtta tatgaaaccg ctgattgaag ctgggaaagt atatatcgcg
     6841 ttaccgcctt tatataaagt atccaaagga caaggaaaga agcaagtcat tgaatatgcg
     6901 tggacagatg acgaattggc agcaatgatt aaaaaagtcg gcaaaggata tatgctgcag
     6961 cgatataaag gacttggaga gatgaatgcg gaacaattgt gggaaacaac gatggatccg
     7021 acttcccgta cgttgatccg cgtccgtatc gatgatgctg cgcaagctga acgtcgagta
     7081 accacactga tgggtgacaa agtagaacct cgaagaaaat ggatcgaaaa tcacgtgcaa
     7141 tttacgctcg aagaagatgg cagtatctta gataaaaaag aggatactga aatctctcct
     7201 tcagtttcta atgatttatt agatgaagaa cgagcagata aaaatgaaaa caatcagtta
     7261 tttgaagtag aataggaggt cacctgatgg aaaatcgaca agaagtacaa gaattaactt
     7321 tagaagaagt gatgggtgac cgcttcggac gttattccaa atatattatc caagaacgtg
     7381 cactgcctga tatccgtgat ggattgaagc cagtgcaacg ccggatatta tttgccatga
     7441 acaaagatgg caatacgtat gacaaaggat tccgtaaatc agcaaaatct gtcggaaata
     7501 tcatggggaa ttatcatcct catggtgaca tcagtattta tgaagcgatg gtccgcatga
     7561 gccaagactg gaagttgcgg gaagtactga tcgaaatgca cgggaataac gggagtatgg
     7621 acggtgatcc acctgctgcg atgcgttata ctgaagccag attatccaag ttaagtggag
     7681 agatgctagc agatatcgag aaagaaacag tcgatctcgt gtggaatttt gatgatacag
     7741 aaaaagaacc aacagtcctt ccagctcgat acccaaacct tctcgtcaat ggatcgacag
     7801 gtatatctgc tggatatgca acagaaatcc cgactcataa cttagctgaa gtgatcgatg
     7861 gcacaatcta tatgatcgat catccacaag cgagccttga aaaattgatg gaatatattc
     7921 ctggacctga ctttccaaca ggagggatct tgcaaggaaa agatgaaatc aaaaaagctt
     7981 atgaaacagg acgaggaaaa gttattcttc gttcgaaaac aaagatagag gcaatgaagg
     8041 gtaataaaca gcaaatcgtg atcacagaaa tcccatatga agtaaataaa gcgaccttgg
     8101 tgaaaaaaat ggatgagatc cgtttgaata agaaaatcga tggaatagct gaagtacgag
     8161 atgaaagtga tagaacaggt ctgcaaattg tcgtcgaatt gaaaaaagac gccaatgctc
     8221 aaggaatttt gaattatttg ttcaaaaaca ctgagctaca gatcaattac aatttcaata
     8281 tggtcgctat cgaccatatg actccgcatc aagttggact gaaagacatt ttaagaagtt
     8341 atatcgagca tcgaaagcaa gtcataacaa aacgtagtca attcgattta gccaaagctc
     8401 aaaaaagaca gcatatcgta gaaggattga tgaaagcttt atcgattctg gatgaagtaa
     8461 tcgcaacgat tcgtgaaagt aaagacaaaa aagacgccaa aaagaattta gtcgacgtct
     8521 ttcaatttac agaagaacaa gcagaagcaa ttgtcacatt gcaattatac cgtttgacga
     8581 acacggatat cacagagctt caaaaggaat ccgaaagctt gattgcgcaa atcacagagt
     8641 tgaataaaat cctttcaaat gacaaagagc ttttctctgt tatgaaaaaa gaattgcgag
     8701 aagtgaaaaa aaattattct tccgctcgtc taacgacaat cgaagacgaa atcgaagaaa
     8761 tcaaaattga tacgcaagtc ttagtggcac aagaagacgt aattgtcagt gtgacgcgag
     8821 aaggctatgt gaagcgaaca agccttcgtt cttatagtgc ttccaaacca gaagaaatcg
     8881 ggatgcgaga aggcgactac ttgctatact caggtgaatt gagtacattg gaccatgttc
     8941 ttctgatcac aaataaagca aatgtgatct accgccctgt ccatgaacta cccgatttga
     9001 aatggaaaga tgctggtgag cacatttctc aaacaatctc caatctgtct gtagacgaat
     9061 cgattttagc tgttttccca tatcaaaaaa ttcaagcaga gaaaacattt gtattcatca
     9121 gcaagaatgg attgatcaag caaacacgaa tgacagagtt cgaaccatgg cgcacctata
     9181 agagccgtcc gctcagctgt atgaagctaa aggcggaaga agatgaactg attgctgtgt
     9241 atctgaaaga agatcagcca ggcttagatg ttttcttagt aacgcatcaa ggaatgggtt
     9301 tgcgctatcc tttaacagat gttcccgttg ttggagcaaa ggcagctgga gtgaagtcta
     9361 tgaatttgaa agatggagat aggattatca acgggctgct tgtgttagca gaaggtgata
     9421 cgccgatcgt aatcgttact cagcgaggag ctgttaaacg catgcttgct caagaaatca
     9481 gccaaacttc acgtgcgaag cggggagtga cggtcatgcg tgagctgaaa aaaaatccgc
     9541 accgtattat ctatatgtcg gaaggaagat caaaagaaat aaccttgatg aatcaaaaag
     9601 ggcagcagtt aacgattgac ccaactgatt tccctatagg tgatcgaaca tcgaatggat
     9661 catttgcgat ggatgagaaa aaaggtggag aagtcatcga agttatcgat gctcctgaga
     9721 ttttcatcga gtcgtaactg ttgtagtatt ttaatgaaga gacgccatct gtattaatac
     9781 aggtggcgtc tctttttcat tcatttttaa taaatcctta tacgtttgca aaaaaagatg
     9841 aaagtgttca caatgcttaa atgttggtat gtcaacgttg atttaaatat atgtaaataa
     9901 aacataaaaa ttgttaaata aaaaacaaga aaacccttgc aaagataggg tgctgtgtta
     9961 tactaatcac gtaaaaactg ttacataaaa cagaaattta ggaggtccat acaatggagc
    10021 aatggaatgg atttaaaggt accaaatgga aagaaggcgt agacactaga gactttattc
    10081 aaaataacta cactgaatat cgcggtgatg acagcttttt ggaaggccca acagaagcaa
    10141 cagaaaaatt atgggatgaa ttgcaaaagt taaatgatat ccaattcgag cgtaacggtg
    10201 tctatgatat ggacacagaa atcgtatcaa ctattacttc acacgatgca ggctatttgg
    10261 ataaagattt ggaaaaaatc gtcggtctac agacagacaa acctttgaaa caagcattca
    10321 tgccgttcgg cggaatcaat atggctaaca atgcgctaaa aagcaatggc tatacaccaa
    10381 acgatgaatt gacacatatt ttcactgact ggcgcaaaac acataatcaa ggtgtatttg
    10441 acgcttatac tgctgaaatg cgtgctgctc gtaaaaacaa aatcattaca gggcttccag
    10501 acgcttacgg tcgcggacgt atcatcggtg actatcgtcg tgtggcattg tatggaatcg
    10561 actatttgat ggaacaaaag aaaaaagatc atgatatgac tggttatcat acaatgagtg
    10621 aagaagtgat tcgtttacgt gaagaaatca ctgaacaata tcgtgcatta gcgcaaatga
    10681 aagaaatggc tgcgaaatat ggctatgata tttctaaacc agctgcaaat gcaaaagaag
    10741 ctgtccaatg gctatacttc ggatatttag cagcaatcaa atcacaaaat ggtgctgcga
    10801 tgtcaatcgg acgtatctca gcattccttg atatctatat ccaacgtgat ttggataatg
    10861 gagtaatcac agaaaaagaa gcacaagaat tgatcgacca cttagtaatg aaattacgta
    10921 tggtcaaatt cgcgcgtaca cctgaatata accaattatt ctcaggattc cctatctggg
    10981 ctactttatc cattgcaggt atgggattag atggtcgttc attagttaca aagaatgatt
    11041 tccgtctgct tcatacatta acaaatatgg gaccttcacc agaaccaaac ttgactgttt
    11101 tgtattctga acaccttcca gaaggattca gaacatatgc tagcagaatc gctatcgaaa
    11161 gttcttcgat tcaattcgaa aatgataatc agcttcgtga acaatggggt tctgatgatt
    11221 gtgcgattgc ttgctgtgta tcaggaacag ttgtcggaaa agacatgcaa ttctttggcg
    11281 ctcgtgctaa cttggctaaa acactattat atgccatcaa tggcggagtc gatgaagtga
    11341 ctggcgccca agttggacca gaatatcgtc caatcacatc agaaatacta gactttgaag
    11401 aagtgaaaca taacttcatg aatatgatgg attggttagc agaactatat gtcaatacgt
    11461 tgaacatcat tcattatatg catgataaat attcttatga agcagctcag ctggctttga
    11521 tggattctga cttgcaacgc acatttgcta caggtattgc tgggatctct catgcggcag
    11581 acagcttgtc agcgatcaaa tatgcaaaag taaaagcaat ccgtaatgaa aaaggaatag
    11641 cagttgattt tgaagtggat ggcgacttcc ctaaatacgg aaatgatgat gaccgtgcag
    11701 atgaattagt ttcatggatc ttggaatact tcatgggaca aatcaaacgt cataaaactt
    11761 accgcaactc aaaaccaaca acttcattat tgacgattac ttcaaatgtt gtttacggta
    11821 aaaatacagg aaatactcct gatggccgtg aagctggaaa accacttgcg ccaggagcaa
    11881 atccaagtta caatgcggaa caaagtgggt tattagctag cctgaactca acagctaaaa
    11941 tgaattacgg ctgtgcattg gatggaattt caaataccca aacaatcaat ccttcaggtc
    12001 ttggaaaaga acaagataca agaatcgaaa acttgcgtaa tgtcttagat ggatacttct
    12061 caaaaggagc ttatcatttg aatgtcaatg tattcagtaa tgatttattg cgtgatgcaa
    12121 tggaaaatcc aatgaaatat ccgaacttga cgatccgggt atctggttat gcagttaaat
    12181 tccgtgactt gacaagagaa caacaattag atgtcttgat gagaacttca cacgacagaa
    12241 tgtaaaaagt tcgtgaaaaa agtttttcga cttagcgtcg aaaggacgtt gaatagccat
    12301 cgcatcaaaa tcgaataatc agtatgctta tttgaaagaa acgatttaca ggggcaggac
    12361 ttgtatgttc cgtccctgta aattttacac ataacgaagc ggcaattgcg aaaaattttg
    12421 ttcagaaagc ttcgcttcga cagctaactt gtacttaaaa tgaaatcaaa gaaaagatgt
    12481 gaacttaaat ggaagaaaaa acaatcggct atgttcattc tatcgaaact ttcggttcag
    12541 tagacggacc tggactgcgc tttgtcgtgt ttatgcaagg atgccgcatg cgttgccaat
    12601 tttgtcacaa tccagatact tggaatattg gcggagggaa agaatacaca gcagatgaac
    12661 ttttagataa agcagaacgc ttcagacctt actggggaga taaaggtggt attactgtta
    12721 gtggtggaga acctctattg cagatcgatt ttttgatcga actatttaaa aaagcaaaag
    12781 agcggaagat gcatactaca ttggatacat gcggaaaacc ttttacgtat gaagaacctt
    12841 tcttttcacg ttttcaagaa ttgatgaagt atacagattt actgttattt gacatcaaac
    12901 acatcgataa tgaagagcat aaaaaactaa cgcattggga caatgaaaat attttagaaa
    12961 tggctcaata tttgtcaaaa atcaacaaac cggtttggat tcgccatgta ctagttcctg
    13021 agcgaagtga ttatgatgaa tatttgatcc gactagacaa ctttattcaa accttgtcaa
    13081 atgtagacag agtagagatt cttccttatc atacgatggg aaaatacaag tgggaaacac
    13141 ttggtttgaa atatccatta gaagggatcg aaccacctac aaaagaacgt gtcgaaaatg
    13201 ctaaacgatt gcttcataca gaagagtata caaaatattt aacgcgatag gtcgcatcgg
    13261 aaagaagata agtattgctt ctttccgttt ttttgctatt attaaatagg agaaatcaca
    13321 aaggagcgaa tgacatgtca aaaattttag tttttggtca ccaaaatcca gatacagatg
    13381 caatcggggc agccattgcg ttttcacact tgcaaaatga attaggtaaa gaaacagaag
    13441 cagttgctct aggtacacct aatgaagaaa cacaatacgc attagaccac tttcagctat
    13501 ctgcaccacg agtagtagag tcagctaaag cagaaaatgc agatgtcatg cttgtggatc
    13561 ataatgaatt ccaacaaagt atttcagata tcagcgaagt aaatatttta gctgtagtag
    13621 accaccatcg tattgcaaac tttgaaacag ctaatccatt gtactatcgt gcagaaccag
    13681 ttggatgtac aagtacgatt attttaaaac tatataaaga aaaccaagta acaattccta
    13741 aagaaatcgc aggaatcatg ctctcagcta tcatttcaga tacattgttg ttcaaatcac
    13801 caacatgtac gcctgaagat gaagcagctg caaaagaact tgcagcaatc gctgaagtcg
    13861 atctaaacac ttatggactt gaaatgttga aagcaggaac aaatctaagt gataaatcag
    13921 ctgcggttct tcttgatttg gatgcaaaaa gtttcccaat gggcagttcc aacgttcgta
    13981 tcgctcaagt caatacagtg gaccttaatg aagtgatgga tcgacaagca gagctagaag
    14041 cagcaatgga agatgaaatg aaagcaaatg gctacgatct gttcgtatta gttgtcacaa
    14101 acatcttaga cagtgattca gaattacttg ttgctggtaa aggaaaagaa aaagtagaag
    14161 aagctttcca aacaactttg accaacaacc gtgctttgct taaaggtgtc gtttcacgta
    14221 aaaaacaagt cgtacctcaa ttgacggctg tatttagcta atcaaatggt agtatgaagc
    14281 attcaaaatg aatggataag cgattgatat aaaaagaaac atagaacata ctaaaggccg
    14341 aagaaatgga ggagaataac agtcaattat gactcttgtt cttcctcctt ttt
//
LOCUS       DACDKL010000060        14393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDKL010000060 DACDKL010000000
VERSION     DACDKL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.060"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(39..935)
                     /locus_tag="IV329_001368"
     CDS             complement(39..935)
                     /locus_tag="IV329_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8940558.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(928..1608)
                     /locus_tag="IV329_001369"
     CDS             complement(928..1608)
                     /locus_tag="IV329_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940559.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1609..2391)
                     /locus_tag="IV329_001370"
     CDS             complement(1609..2391)
                     /locus_tag="IV329_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8940560.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2384..3301)
                     /locus_tag="IV329_001371"
     CDS             complement(2384..3301)
                     /locus_tag="IV329_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8940561.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3465..3788)
                     /locus_tag="IV329_001372"
     CDS             complement(3465..3788)
                     /locus_tag="IV329_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8940562.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4576..7803
                     /gene="fss3"
                     /locus_tag="IV329_001373"
     CDS             4576..7803
                     /gene="fss3"
                     /locus_tag="IV329_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8940563.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7916..8230
                     /locus_tag="IV329_001374"
     CDS             7916..8230
                     /locus_tag="IV329_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8940564.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8243..8617
                     /locus_tag="IV329_001375"
     CDS             8243..8617
                     /locus_tag="IV329_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8940565.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8627..8962
                     /locus_tag="IV329_001376"
     CDS             8627..8962
                     /locus_tag="IV329_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8940566.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9045..10385
                     /locus_tag="IV329_001377"
     CDS             9045..10385
                     /locus_tag="IV329_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8940567.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10463..11137
                     /locus_tag="IV329_001378"
     CDS             10463..11137
                     /locus_tag="IV329_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8940568.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11370..11804
                     /gene="dcm"
                     /locus_tag="IV329_001379"
     CDS             11370..11804
                     /gene="dcm"
                     /locus_tag="IV329_001379"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8940569.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            11805..12512
                     /locus_tag="IV329_001380"
     CDS             11805..12512
                     /locus_tag="IV329_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8940570.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            12502..12792
                     /locus_tag="IV329_001381"
     CDS             12502..12792
                     /locus_tag="IV329_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940571.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13051..14235
                     /locus_tag="IV329_001382"
     CDS             13051..14235
                     /locus_tag="IV329_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8940572.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14250..14369
                     /locus_tag="IV329_001383"
     CDS             14250..14369
                     /locus_tag="IV329_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8940573.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         4600 a         2634 c         3019 g         4140 t
ORIGIN      
        1 aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg caacttgacg
       61 gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc
      121 aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt
      181 tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg tttgatagga
      241 ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca
      301 tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga
      361 tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca
      421 gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta
      481 tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc
      541 atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc
      601 gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg
      661 agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct
      721 tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag gtccattaga
      781 ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg
      841 gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata
      901 aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc cccacaccgt
      961 atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa tatgcatatt
     1021 cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg
     1081 cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt
     1141 caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc
     1201 cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg
     1261 gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat agtcatccgc
     1321 tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat
     1381 cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag
     1441 catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt
     1501 acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaggaatg
     1561 aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct acttaatgtc
     1621 agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac
     1681 cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag
     1741 ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc
     1801 gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact
     1861 tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca gcagaagcaa
     1921 tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa
     1981 tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca cgccaagtgt
     2041 tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa
     2101 agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa
     2161 tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa tccctgttgc
     2221 accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata
     2281 ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat
     2341 tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc
     2401 ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga ataccatcca
     2461 caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca attccttcaa
     2521 aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga gagtcgagaa
     2581 ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg
     2641 ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata ccatagtgag
     2701 tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt
     2761 cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat
     2821 tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta
     2881 aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc
     2941 gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga
     3001 tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac
     3061 ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat
     3121 caatcgagat caaacccata atagttctca taaaggtaga cttacccgct ccattttccc
     3181 caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct aatgctttgg
     3241 cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca
     3301 tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa agaaagcagc
     3361 cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa caatcggaca
     3421 atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt tcctgattct
     3481 ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt tgcttctttc
     3541 agatcaagct tgagtaattt taccagaccg gttataagga taactatagc aagactccct
     3601 cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca
     3661 ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacata
     3721 agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa
     3781 gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac
     3841 aattagctac caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata
     3901 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag
     3961 caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa
     4021 gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa
     4081 tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt
     4141 tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt
     4201 cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag
     4261 aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc attgaataag
     4321 tttgaaaaac cttgatttat aaaggttttc ccgtaaatat attttattat atttgattaa
     4381 atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa ggcagtatat ttcgtatttt
     4441 agattaaatt tttaggctca ttcattctca aatccattct attcagtcaa taaaaatttt
     4501 aaaaagactt tataaacaaa gatgttcaag cgagcatctt atttttttgt gcttaatttg
     4561 aaaggagaac atttcatgta taaaaaacaa aaaaagaaat tatgtgtatt aactattttg
     4621 agtttggctg ttcaagcaat tgttggagca attgctccta cgattacttt tgctgatgaa
     4681 attacccatc cacaaacggt aaccgtagaa ctagatttag cacatcaata tgctgttgaa
     4741 ggaactttta gtgatggtcg tccaatgtca gaagtcactg ttccgcatta tgcagtgtac
     4801 aacggcttta aacaagatat tttctgtatt gaaccaggag tcccgattta caatgagttc
     4861 actcctggct atgagaaaaa cccgttacca gatatgtccg ataaagccaa attagtttcc
     4921 gttttatgga aaaaagtagg tacagatgca gatactcata ttgtggcaca gaagatgatc
     4981 tggcaagaag ttaatgggta tacccttcac tcaataaaac gatcagatgg tagtgccgta
     5041 aatatcgcag ccattgaggc aaaaataaat caagccattg acgattatca gaagaaacca
     5101 accttccata atagtacggc taaaacagta ttagggcaat cgactactgt gacggatacg
     5161 aataatctga atttgtcaga gttcgatgag gttgtggaaa atgccgcaaa cattgattat
     5221 cgtgtgaatg gtaatcagtt gataatcacc ccaaatgcca attcaaaaga aagtggtgtg
     5281 ctgacgctta agaaatctgc tggtaccgga acacctgtcg cctataaaaa ggctggccaa
     5341 cagactttaa tggccggggc gattgataaa cctaatacct acacaatcaa aattgatgtg
     5401 gaaacggagg gttctttaaa gatcaaaaaa gtcgataaag aatcgggtga tgttgtacca
     5461 ggaacggttt tccatttaga ctttggaaaa actttacctg caaaagacgt gactactgac
     5521 aaagaaggca ttgctacatt ggatgagatt cctcatggta caaaagtaac cattaccgaa
     5581 aaatcagtac cggcgcctta tacgattgat accaccccca tgactaccac cattaaagca
     5641 ggcgaaacca tttacgtgac ttcaaaaaac gcacgggaaa aagggcaaat cattctggat
     5701 aaaagcggag ttgaaacagg gagtgatctt tggaatgaca attattcctt agctggtaat
     5761 acgtttgcca ttcgcaaaga cagtccaacc ggtgaaatcg ttcaagaaat gaccacggat
     5821 gaaaacggtc atgcggaaac accaaaagag attgctaatg cgttggaatt gggaacctat
     5881 tatgtgaccg aaactaaagc tagtcatggg ttcgtgaata cgttcaaacc tgtaaaagtc
     5941 gaattgaagt atgccaatca aaccgtagcc cctgttacca gtaacgtaaa agggcaaaat
     6001 caagaagtca ctggcgaaac cactttgacg aaagaagaca aagatactgg ggataaagca
     6061 caaggtaagg cagtctttga gggtgctgaa tacaccttgt ttactgctaa ggatggaaaa
     6121 gctgttaaat ggagtgaggc ttttaaacca gagttagcga agggaacgaa aggttctgat
     6181 gaaacggtca ctttgacttt agacgaaaag aaccaagttg ccgttaaaca tttagctatc
     6241 aacgagtatt attggcaaga aacaaaagcg cccgaaggat atagccttga tgaaacaaag
     6301 tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa atgccgtgat tacccgaaat
     6361 gttacggcaa aagaacaagt gattcgcttt ggctttgatt tctttaaatt tgctggatca
     6421 gctgcgggca ctaccgaaac tggcttcaat gatctgacct ttaaagtgtc gccattggaa
     6481 gggaccaatg aaatcacagg tgccgaagat gaagcgacca cagcttataa cgagcaatta
     6541 gggtttgatg gttatggcaa gtttgaaaat cttccttatg gggattattt acttgaagaa
     6601 gtagaagccc cagaaggttt tcaaaagatt acgccattag aaatccgttc tacttttaag
     6661 gaaaacaaag aagactttgt gaagagtgag tatgtcttta ccattaccga acaagaccaa
     6721 aaacaaccaa ttaaaacggt gaccgttcct tacgagaagc tgacgaacaa agcattttct
     6781 gttagtttga accgtttaat gctctatgat ttgcctgagg aagaagatag tttaacttct
     6841 cttgcgactt ggaaagacgg aaacaaagaa ttaacttctc tggattctac cgagctagtt
     6901 gataaattga gttacaatct acatgaaatc aaagaagact ggtatgtggt agcgcaagcc
     6961 attgatgtgg atgcgacaaa agctgcccaa gaaaaagatg aaaaagccaa accagtggtg
     7021 attgcagaaa caagcgcaac gttggcgaac aaagagaaaa caggaacttg gaaaattcag
     7081 cataaattaa ccgccgaaca agttttaaat aaaactattg tcttattcaa ctatgtgtat
     7141 gaaaataagg aagcttttga agcaggcgat aagccagtag cgaaggatgt tagcttgaat
     7201 aaccaagccc aaactgtcag ttgtacagtg gaacaccatg tttctatcca aacaaaggcc
     7261 cacctagaaa acggttctca gacctttact catggtgatg tggtggatat gtttgatgat
     7321 gtgtcgatta ctcatgatgt actggatggc tcaaaagaag ctttcgaaac aattctgtat
     7381 gctttattac cggatggtac aaacaaagaa atttggaaat ctggcaagat tgattatgaa
     7441 gtaaatgata aagaattcac caaaactgtg cttgcgaaaa aagtagatac cggaaaatat
     7501 ccagaaggaa ccaagtttac ttttgcggaa atcaattacg ataaagatgg gaatatcaat
     7561 ggaaaacaca atgaagattt gaaagaaaaa tctcaaacct taacaccaaa agaagttcca
     7621 accatactaa gtacgccgaa acaaccggaa acaccaactg ttccaagtgg ctctcaagaa
     7681 tctagtccca cagtgaagac cttccctcaa accggggaga aaaattccaa tgttttactg
     7741 ttcattggtt ttactttgat ttttgcgacg gcgggctatt atttctggaa tcgccggaac
     7801 taaggtgatg agatgataca agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa
     7861 aaagtagtcg cgctattttt gtatccaaaa ctaacaatga aaagaggaaa agaagatgga
     7921 attgaaattt gtcgtgccag atatggctga aacctttgga aagttaagtt atgccggtga
     7981 aggagaaatt ataacagaag gatatggacg gaatactacg gtgattggtc gcagttatca
     8041 tttgtattcc agtaagcaac gggccgatga tattgaggtg gtagtagctg cggaagccgg
     8101 tgaaaaggat tttgatcaag accaaccgct aaaagccgtg aatccccatt tggttgccaa
     8161 aggctatgag attgagaatc gtgggtttac tgattacgtg ttgtatgttg atgatttagt
     8221 gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag attcaggttt
     8281 aacatttggg aagttgcgtt tttccgcatt acgccgagaa gtacggaagc aaaatgagga
     8341 tggaacaatt ggtaatgagg taaaagaacg aacctataat ttaaaatcct ctgcacaagg
     8401 gcgaatgatt caagttagta tcccagctaa tgtaccctta cgcgagtttg cttatgatgc
     8461 agaggtagag ttggtcaatc caatcgtgga taccgttgcc aattatgttt ttcgagaagg
     8521 aactaccgtt aattggttca ttaaggcgga tgatttagtc ttgaagcggc agccgaatca
     8581 aggaaatcct acgaaccaaa atgaaagaaa gaaataggtg aagattatgg aaattaatgt
     8641 tgaacatatt ttagattgtc tcgatcaata tggaaaaggc gagctgactg aggaacaact
     8701 cgttaaagca ttaacctctg atgaaaaaat gtttctgatt atacatcaag gtttacttgg
     8761 agaaaatagc gacagtgaag aagactctga tgtactagac tggttaggag aagaagagtc
     8821 tttctttata gttgtcgaag taaatgaaaa cttatgtcgg caggcagaat ccattttgga
     8881 agaaattggt gtggagatgc cggatgctat tgaaggcttt ttaaatcaat tggttgaaac
     8941 gaaacaactc ccctcctcgt agcagtaaat gactagcata actttttgaa attgcctaat
     9001 gaaatcctat tgggcttttt cgattggagg tggaaaccat caaaatgtac aaaggacatc
     9061 gcatacgcgc aggagatcag cacttggttt atcactttgt tcttggctgg ctactcgctc
     9121 tgttcatcgg ttggatgagc gtcttttatt ttcaagagtt tagacaattt gatatttcta
     9181 agctatcact ttctactatt gaaatcgttt ggtctataaa agatttagta tgtctgcttg
     9241 gaagtctcgc tttttcagga gctatgatat tgctttatat tcactttttt cttgatcatt
     9301 ggcgaagctt gtggcatcga caaaagctag ctcggatgat tctagagaac cattggtatg
     9361 aagtgaaaca aactcaaagt gaaggctttt tcaaagattt gaatagtagc cgaaccagag
     9421 agactatcag ttacttcccc aaaatctgtt atcgaatgaa agatggatta ctgtctattc
     9481 gcgttcaaat ttcacttggg aaataccaag atcagctttt aaaattggaa aagaagttag
     9541 aaagcggact gtattgtgag ttagtagaaa aggaactcaa agactcctat gtggagtata
     9601 ctttactgta tgatatgatc gccaatcgaa ttgggataga cgaagtagtg gcagaaaatg
     9661 ggactctgcg attgatgaaa aatcaagtat gggcttatga ttctttaccc catatgttaa
     9721 tcgctggtgg cacgggtgga gggaagacct atttcctcct caccattatc gaagcactat
     9781 tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa tgcagattta gctgatttag
     9841 gtacggtgat gcctcatgtt tattcacaaa aggaagaaat ttctgcttgt gtggaagatt
     9901 tctatgaacg tatgatggct cgtagcaagg caatgaaaga aatgtctaac tacaaaacag
     9961 gagagaatta tgcctatctt ggacttccac ccaacttttt aatctttgat gaatacgtgg
    10021 cttatatgga gatgttaacg acgaaagaaa gtgcggtgat tttgaataag ctcaaacaaa
    10081 tcgtgatgtt gggtcgtcaa tctggtttct ttctgatttt ggcttgccaa aggccggatg
    10141 cgaaatattt aggagacgga atccgcgatc aattcaactt tcgcgtggct ttgggtcgta
    10201 tgagtgaact cggatattcc atgatgtttg gggaggtcga aaaaaacttc tttatgaagc
    10261 acatcaaagg tcgaggttat gtggatacag gtggcagtgt gattagtgag ttttatacgc
    10321 cacttgtacc aaaagggtat gatttcttag attcaattag taaattagaa aagatgaatg
    10381 attgagtgat cttcaatact gactttatac ttcaagtaac gtataatact tttatatagt
    10441 aggaagaaag gggataatta atatggaaag taatgattct tatttagagt atgcaaaagc
    10501 aaataaggat aagtttattt cttatattat caaagggaaa tcccccgaag ttggacgaga
    10561 cgcagttttt atggcaggga gtcctggggc tggaaaatca gaggttgcca ttggattagc
    10621 tgagaattat gataaccatg tgattattga tgcggattac tttcgcaccc agtttcctga
    10681 ttataatgga aaaaactcta gtctctttca aaaagcatct tcttggttgg tagagcaggc
    10741 atttagatat gtcttagatc atggctattc ttttattctg gatgcaactt ttgcaatatt
    10801 aagtgctgaa aaaaatatct cgcgggcgtt gaaaaaacat tttcgagtga cgattttcta
    10861 tgtttatcaa gaccctaaaa tcgcttggaa atttacacaa gaacgtgaaa ttgcagaggg
    10921 acgtcatgtg ccgaaaaagg catttattaa cgcttttttc aaatcaagag agaacattgc
    10981 aaaagtaaag agtaaacatc cagatgtatt gcttcatatc ataataaaag attatacgaa
    11041 taatatttcc gaagttcatt atgctacgga taatctagaa ttatttttac cactagggta
    11101 tagtaggaaa gatttggagg ttcaattaca tgactgagaa agaacaagta acgaaaattg
    11161 taaagaaata caataagagc attgcggatt tatcggaaaa tgctactgca aaagagttca
    11221 aaacggtgat taaatatgta gccgatcagg ccaatgaaaa acaacggaaa ttagttggat
    11281 taaataaaaa atagtaggac aaattaataa tgtatgagcc aagagcaaaa tgttcttggc
    11341 ttttttcttt gcaatgaatg aggtaatgta tgagattttt agatttattc gccggtattg
    11401 gtggctttcg tttaggtatg gaacaggcga gccatcattg tatcggtttt tgtgaaattg
    11461 ataaatttgc tagacgtagt tataaagcaa ttcatgatac aagtaaggag gtggaaatgc
    11521 atgacatcac aagtgtatca gatgagttta ttcaatccct cggaccagtg gacatccttt
    11581 gcggcggatt tccgtgccaa gctttttcaa ttgcgggaaa gcggcaaaga ttttccgata
    11641 ctcgaggtac tttattcttt gaaatcgctc ggttcaccac tcttctccga cctaagcttt
    11701 tattccttga gaacgtccga ggattgctca atcacgaagg aggggctacg ttcgagacga
    11761 tcctccgaac gctggatgga ttggggtatg acgtggagtg gtaggtgctt aactcaaagg
    11821 cctacattcc ccaaagccgg gagcggatat tccttatcgg acattctcga gacgcatgta
    11881 ccgaacaagt atttcctatc attggaccgt cttcaacatc tgatcaaaac atcagaaatt
    11941 tggtaaatat caatccttcc aatcgaggaa tgggtggaca agtctatggt tcagatggcg
    12001 tagcgccaac cttaacaggt gatgaaggaa tcaaaatcgc actgccagca agggatggaa
    12061 tctctgtagc aggactatta ccgggaaagt ttgaacaagg taatcgagtt tatgaagtta
    12121 ctggaaccgc ccccaccttg tcaacgaaac aaggtggtac aaaaatcatg attcgaaaaa
    12181 atgaaacaga atatcaagag gtgaagccag aagacagtgt gaatctggct tttcctaatt
    12241 ccacaagtcg ccgaggtcgt ctgggaaaac agagtgttca taccttacta acaggagacc
    12301 aacaagcgat cgtcacggat cagtatcaaa ttcggaagct aacacctaga gagtgttggc
    12361 gacttcaggg ctttcccgat tgggcttttg atcgagattc tcaagtcaat tcagatagtc
    12421 agttgtataa gcaagccgga aactctgtaa cagttccggt catttttgat attgccagaa
    12481 gattaaaaga gttggatgta catggtttat gatgagttga ttggagaaat ttattgggtg
    12541 attggaaaga ttcagtcaga cccagaatta gaagaacaat tgcgacggtt aaaatttgat
    12601 attcgcaaga atggtgtcaa agtcgctggc gatccatatt taatgaatga ggagacggat
    12661 gctcgaattg aaataaatca agtaattgca gaatttgagc ggattgcgga ccaagcaaaa
    12721 gaaccagata ttcgtcaata cttgttagaa atgaaagcag agttggaagt aaatggtatt
    12781 acagatgagt aacctaaacc aacacaactg actgttaggc gctgaccgcg gaggattcgc
    12841 gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac ggcgttagcc gaccatgatg
    12901 tctcaccccc gtatctaaca ggggggtaca aaaacacaaa aaataagtaa gcaaccatta
    12961 agaatgcttg ttgtatcaaa gatagtaagt aatttgtata ggtgttaact catttgaaaa
    13021 agttaacacc ttgaatttgg aggtgagaac ttggcacaac ggaatttaga ttatcggcta
    13081 ttaaaagacc gacgcaacga atacggcgtt tcacaaaata aattggctac ggcttgtgga
    13141 cttagtcggc cgtacttaaa tcagatagaa aatggtggcg taaccgcatc tataaaaacc
    13201 atgaggaaaa tttttgacca actggaaagc tttaatcccg atttgccttt gacgttactg
    13261 tttgactatg taaggattcg ctttcccaca acggatgcac gaaaaattat tcaagaaatt
    13321 ctccatttga aatttgagta tatgctccat gaagattatg ccttttactc ctatcaagaa
    13381 cagtatgtca tgggtgatat tgtggtgatg ttgtctcatg aagaagataa aggtgtgctt
    13441 ttagaattaa aggggcgagg ttgccgacaa tttgaaactt ttttactcgc tcaaaagcgt
    13501 agctggtacg actttttcga ggattgtcta aaggccggtg gcgtgatgaa acgcttggac
    13561 ttggcaatca atgatcgagt ggggctattg gatatacctg agctaacgaa aaaatgtcag
    13621 aaggaagaat gtatctcctt gtttcgtacc tttaaaagtt atcgttccgg ggaactttta
    13681 aaggctgatg aaaaggacgg catgggcaat accttatata tcggcagtct caagagtgag
    13741 gtttatttct gtttgtatga aaaagactat gaacaatata tcaagttagg gattccatta
    13801 gaccaaactg agacgaaaaa tcgttttgag attcgactga aaaatgaccg tgcttttcat
    13861 gcgattcaag atttgttaaa aggtcgtagc attgagagca ccacgttttc cattatcaat
    13921 cgttacttgc gatttgcgga taaagtagaa ggtaaaaggc gaaccaactg gcccttgaat
    13981 gaacaatggg ggcgttttat tggacggaat cggaaagaga ttcaattaac ttccgagcca
    14041 aaaccgtata ccattgaacg aaccttgaat tggctaggcc gacaagtcgc ccctacgtgg
    14101 aaaatggcta aggagttgga tcgattgaat cggacaacct atatccagga tatggtacag
    14161 aatgcacgct tatcagaccg acataaaaag attttggagc aacaaagtat ggcaattgaa
    14221 aatttgatta tatgaaaaga ggaatcgtca tgggaattgt aaaagatatt tttttggtat
    14281 ttggtggcag tatgcttggt gttactgtga tgtgcttgat gaatgcaagt gcagcagcgg
    14341 atcgagcaat ggaaaagaaa ggaaagtgac aagatgaatt tcggacaaaa ttt
//
LOCUS       DACDKL010000061        14357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDKL010000061 DACDKL010000000
VERSION     DACDKL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.061"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(269..3922)
                     /gene="rpoC"
                     /locus_tag="IV329_001384"
     CDS             complement(269..3922)
                     /gene="rpoC"
                     /locus_tag="IV329_001384"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8940574.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(4001..7618)
                     /gene="rpoB"
                     /locus_tag="IV329_001385"
     CDS             complement(4001..7618)
                     /gene="rpoB"
                     /locus_tag="IV329_001385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8940575.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTYKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8346..9314
                     /locus_tag="IV329_001386"
     CDS             8346..9314
                     /locus_tag="IV329_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8940576.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9332..10237
                     /locus_tag="IV329_001387"
     CDS             9332..10237
                     /locus_tag="IV329_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940577.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10234..11040
                     /locus_tag="IV329_001388"
     CDS             10234..11040
                     /locus_tag="IV329_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8940578.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11197..12165
                     /locus_tag="IV329_001389"
     CDS             11197..12165
                     /locus_tag="IV329_001389"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8940579.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12482..13006)
                     /locus_tag="IV329_001390"
     CDS             complement(12482..13006)
                     /locus_tag="IV329_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8940580.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13293..>14357
                     /gene="scm"
                     /locus_tag="IV329_001391"
     CDS             13293..>14357
                     /gene="scm"
                     /locus_tag="IV329_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8940581.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4076 a         3089 c         2816 g         4376 t
ORIGIN      
        1 attcgaggaa ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc
       61 atcaatttat tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt
      121 ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc
      181 cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata
      241 atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg
      301 cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt
      361 cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta
      421 taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg
      481 tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg
      541 atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta
      601 gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat
      661 ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga
      721 tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca
      781 agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc
      841 cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg
      901 tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac
      961 gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt
     1021 gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat
     1081 cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac
     1141 cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg
     1201 caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag
     1261 aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa
     1321 tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg
     1381 tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg
     1441 cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg
     1501 ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg
     1561 tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac
     1621 ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac
     1681 gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca
     1741 tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg
     1801 cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac
     1861 ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt
     1921 cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt
     1981 accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga
     2041 agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct
     2101 ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga
     2161 cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt
     2221 tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg
     2281 gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt
     2341 tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc
     2401 cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgagaa ggagtaacaa
     2461 ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt
     2521 ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg
     2581 cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg
     2641 gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt
     2701 gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac
     2761 gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg
     2821 gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag
     2881 gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac
     2941 ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg
     3001 gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca
     3061 gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca
     3121 aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac
     3181 gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca
     3241 taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc
     3301 ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg
     3361 cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag
     3421 cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt
     3481 gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga
     3541 tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt
     3601 taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc
     3661 gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga
     3721 tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac
     3781 agaacaaccc ttcacgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta
     3841 cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt
     3901 cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa
     3961 gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg
     4021 cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct
     4081 tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca
     4141 aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga
     4201 cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta
     4261 cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga
     4321 ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt
     4381 ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca
     4441 atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga
     4501 ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt
     4561 cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa
     4621 ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac
     4681 ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg
     4741 aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca
     4801 tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt
     4861 tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc
     4921 ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc
     4981 cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga
     5041 tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc
     5101 ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta
     5161 aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta
     5221 attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat
     5281 cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca
     5341 tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt
     5401 ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt
     5461 agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt
     5521 tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac
     5581 ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg
     5641 gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac
     5701 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt
     5761 taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag
     5821 cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt
     5881 gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac
     5941 gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt
     6001 tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg
     6061 gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac
     6121 gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtatgtcaat tcgccaagag
     6181 ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg
     6241 ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg
     6301 acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta
     6361 aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc
     6421 cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa
     6481 ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt
     6541 ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt
     6601 ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca
     6661 tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa
     6721 ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta
     6781 ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca
     6841 gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt
     6901 ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg
     6961 tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc
     7021 ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag
     7081 agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag
     7141 aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac
     7201 ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg
     7261 tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt
     7321 ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt
     7381 ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc
     7441 cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga
     7501 accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga
     7561 ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc
     7621 tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt
     7681 tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc
     7741 aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat
     7801 taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac
     7861 ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt
     7921 tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt
     7981 gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg
     8041 cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt
     8101 agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat
     8161 ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc
     8221 agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt
     8281 tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa
     8341 aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag
     8401 gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat
     8461 tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag
     8521 cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca
     8581 atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac
     8641 ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga
     8701 ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg
     8761 gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt
     8821 taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact
     8881 ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag
     8941 tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg
     9001 tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg
     9061 aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg
     9121 gcggtctagc aacagtagga atcgactacg aaagcttggg caaacaaaca gggatcatgg
     9181 cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt
     9241 tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca
     9301 aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc
     9361 atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg
     9421 tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc
     9481 ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg
     9541 gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct
     9601 tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc
     9661 gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat
     9721 aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt
     9781 acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct
     9841 ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat
     9901 ttctaatgga tgcattgctc tttctggctc cttacttgcg caaaacaacg gctttgctga
     9961 tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt
    10021 tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat
    10081 ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact
    10141 tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca
    10201 tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat
    10261 ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat
    10321 ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct
    10381 actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat
    10441 ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc
    10501 caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct
    10561 atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt
    10621 ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc
    10681 ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt
    10741 ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg
    10801 gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact
    10861 catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg
    10921 atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg
    10981 ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa
    11041 tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct
    11101 ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc
    11161 ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt
    11221 caattattga aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa
    11281 gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt
    11341 gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc
    11401 cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa
    11461 tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc gaagttattt
    11521 gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct
    11581 ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct
    11641 caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt
    11701 tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt
    11761 ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc
    11821 gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc
    11881 tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg
    11941 caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa
    12001 tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt
    12061 gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt
    12121 ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca
    12181 aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct
    12241 tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata
    12301 aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac
    12361 acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa
    12421 cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc
    12481 cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag
    12541 catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt
    12601 caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag
    12661 cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt
    12721 gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt
    12781 cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta
    12841 attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt
    12901 ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat
    12961 cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt
    13021 tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca
    13081 cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa
    13141 gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct
    13201 ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata
    13261 ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc
    13321 tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca
    13381 ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa
    13441 ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg
    13501 gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag
    13561 gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag
    13621 gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag
    13681 cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg
    13741 atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat
    13801 tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag
    13861 attttccaga catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt
    13921 tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc
    13981 caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt
    14041 caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata
    14101 tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa
    14161 caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag
    14221 gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca
    14281 gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg
    14341 atagctcaga ttcatca
//
LOCUS       DACDKL010000062        13852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDKL010000062 DACDKL010000000
VERSION     DACDKL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.062"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(235..597)
                     /locus_tag="IV329_001392"
     CDS             complement(235..597)
                     /locus_tag="IV329_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940582.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(671..1051)
                     /locus_tag="IV329_001393"
     CDS             complement(671..1051)
                     /locus_tag="IV329_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8940583.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1133..2308
                     /locus_tag="IV329_001394"
     CDS             1133..2308
                     /locus_tag="IV329_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8940584.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2385..2969)
                     /locus_tag="IV329_001395"
     CDS             complement(2385..2969)
                     /locus_tag="IV329_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8940585.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3170..4168
                     /locus_tag="IV329_001396"
     CDS             3170..4168
                     /locus_tag="IV329_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8940586.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4280..4789
                     /locus_tag="IV329_001397"
     CDS             4280..4789
                     /locus_tag="IV329_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8940587.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4819..5478)
                     /locus_tag="IV329_001398"
     CDS             complement(4819..5478)
                     /locus_tag="IV329_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8940588.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5480..6244)
                     /locus_tag="IV329_001399"
     CDS             complement(5480..6244)
                     /locus_tag="IV329_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8940589.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6252..6929)
                     /locus_tag="IV329_001400"
     CDS             complement(6252..6929)
                     /locus_tag="IV329_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8940590.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6966..8351)
                     /locus_tag="IV329_001401"
     CDS             complement(6966..8351)
                     /locus_tag="IV329_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8940591.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8320..8574)
                     /locus_tag="IV329_001402"
     CDS             complement(8320..8574)
                     /locus_tag="IV329_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940592.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8561..8645)
                     /gene="ffs"
                     /locus_tag="IV329_001403"
     ncRNA           complement(8561..8645)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV329_001403"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8668..9183)
                     /gene="tadA"
                     /locus_tag="IV329_001404"
     CDS             complement(8668..9183)
                     /gene="tadA"
                     /locus_tag="IV329_001404"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8940593.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9290..10147
                     /locus_tag="IV329_001405"
     CDS             9290..10147
                     /locus_tag="IV329_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8940594.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10228..10563)
                     /locus_tag="IV329_001406"
     CDS             complement(10228..10563)
                     /locus_tag="IV329_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940595.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10630..12000)
                     /locus_tag="IV329_001407"
     CDS             complement(10630..12000)
                     /locus_tag="IV329_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8940596.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12078..12614)
                     /locus_tag="IV329_001408"
     CDS             complement(12078..12614)
                     /locus_tag="IV329_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8940597.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12923..>13852
                     /locus_tag="IV329_001409"
     CDS             12923..>13852
                     /locus_tag="IV329_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8940598.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQ"
BASE COUNT         4234 a         2774 c         2257 g         4587 t
ORIGIN      
        1 gttaaattta tcatgtattt cttccttggt caactataaa ttaagcatta ctttgtgaaa
       61 tgatttttcg atcaattatt attattcgtt atacaaccgt tttcaacata ttttgaaaat
      121 aaaatgcact aaaaaactat ctttagcaga acatttcatt gtattttttg gaccaaacaa
      181 taaaaaaaga agtttgaaaa aaattcaaac ttctttgttc gcttaatctg tattttagaa
      241 gaaaggcact ccctctactt cgtaagttcc aagaacagag cgcatttgac ctggttcttc
      301 ccaaatatat ccttcgccta attcttctct ttgattcttt tgttcagttt caaagacaaa
      361 caacggaaca ttttcctctt taatatgtgt atttgtcaat tccatcaaac taatcttgct
      421 aacatcgata tgaactattt cttttaagaa attaagtata ttcgctaaac cagtctttcc
      481 ttcagaaagt tccgctaacg caaattccaa cttgtcatca attgaatgga ccaaaaattt
      541 tttagtacca tcctccaaat gcaacataat tgtaccagca actcttttgc ttgccaaaat
      601 ttccacctcg atcaacttgt tctatggctt acataaaaat tctagcacat tttcagaaaa
      661 aatacacttt ttatttctta ttttctttcc gttctagttc agataatgct tcttctatcc
      721 aaataggtaa ctgctccgca atagtggtaa atccgatctt acgattttta ttcgtgaaat
      781 acctttttct gcgatatgga acagctggat ttgttttttc taacgttctt aaagataaac
      841 tgattttttt ggaatattca tcaatgtcga tgatctgaac tgttactggc tgtccgactt
      901 ttaacaatga atggatattt ttcgtataac cggattgtac ttctgaaaca tgaataagtc
      961 cttgcgtctc gttatcaaga gaaacgaatg cgccataagg ctgtatccct gtcacttttc
     1021 cttcaattat ttgtccaatt ttataagtca tctacttccc tctcttcgca tttatcatta
     1081 tactgtaaat agctcaaaaa aagaagcgac gaaaatgact ggaggtctta agatgatcga
     1141 atttgataaa cagatttcaa gaaaagatac agaaagtgta aaatgggacg caattgctga
     1201 gacatatcaa atggatgact tacttccttt gtgggtagca gatatggact tcttgtctcc
     1261 cggtggggta gctaaagcgt ttagtgaata catcaaacat ggaatctttg gttatgccac
     1321 tctgtctgat aaactatatc aggctatcat tcattgggaa cgccaacacc atgctgtgga
     1381 gttgacgaaa gaaaatatcg tttttacaag tggtgtgtta accagtttag ctgcagcagt
     1441 acaaactttt acaaaaccag gcgattctat tttgattcat gacccagttt atcctccatt
     1501 ttcctctatt atagaaacaa atcaacgtca catcgttcga agctctttgc tagaaaaaaa
     1561 taatcatttc atcatggact tgtctgacat ggagaaaaaa atcgaagaaa acaatgtaaa
     1621 agcaatgatt ttatgcaatc ctcacaatcc tggagggcgt gtttggagca aagaagaatt
     1681 gaaacagtta agcgaattgt gtttaaaaca tcaagtgatt ctgtttagtg acgagatcca
     1741 tcaagattta actttattcg atcatacatt tacttccatg ctaacgattg atcctaattt
     1801 agatgatcta ctagttgctt ttacttccgc aaccaagact ttcaatctag ctgcaatcaa
     1861 aaattcaatg gtatttatca aaaatccaga gatgaaagct gattttgaaa aacatttagt
     1921 gatgaaccaa cagcacgaaa tcaacacttt tggactgatt ggaacccaag cggcttatga
     1981 gacgggtgga gaatggttaa gccaactgat tccttatcta gaaaaaaatg ttcaaacagt
     2041 taatcagttt tttgaacagt atcttccgaa agtgagaata atgaaccctg aaggaactta
     2101 cttaatgtgg ttagatttta ccgcctatat ttcagacgac caagaattgg agaacactct
     2161 tgttcggaaa ggaaaagtcg tattaaatcc tggcatcacg tttggtcctt ccggtcatgg
     2221 gcatatgcgc ttgaatattg cttgtccaga agaaacttta ctggaaggac ttaatcgaat
     2281 caagtatgct ttagagacaa tcgattaaat catttattca aaataaaagg acgagacctt
     2341 actaggtttc gtcctttttc ataattactc cgtaccatac caaactattc tgaaatttca
     2401 attgtttcaa tcactacatc atgtacaggt cggtcttgtg gaccacgttg tacatttgcg
     2461 atttcatcta ctgtgtccat cccttctagt acatgtccaa agactgtatg tcggaaatct
     2521 agccaaggag ttccgccacc tttatacgct tcaatgattt ctgatgggaa tccagcacct
     2581 tctagttgtc caagcatatt agctggcaca tttgtgttat ttacaataaa gaattggctt
     2641 ccgtttgtgt ttggacctga atttgccata gataaagctc cgcgaaggtt gaacagttca
     2701 cgactgaatt catcttcaaa actttcgcca taaatacttt cgccacccat tcctgttcca
     2761 gttggatcgc cgccttggat catgaaatca ggaataacgc ggtgaaaaat cacgccatca
     2821 taataacctt ttttggaaag ttcaatgaaa ttttgaaccg tcttcggtgc tagttcaggg
     2881 aataattgaa cagtaatgtc accacggttt gttttgataa cagcttttgg tcctttttca
     2941 ctttttaaat ctagttgtgg gaatgccata ttgtttcctc ctaatatttg catacttcat
     3001 aataataata acataatacc taacctctcg taaaaatact agatttgttt tcttttattt
     3061 ttcatcacta gatacattct ataacttacc gtttcgattt cgaattcaaa catttttgag
     3121 aagcattctc aattaaattc tgctatagtg tgaaaaggga ggaattttta tggaaatcta
     3181 tgacattaca atcatcggtg caggtcctgt agggatgttt gccgcttttt acgcaggaat
     3241 gcgccaagct aaaacaaaaa tcattgacag tctacctcaa cttggtggtc aattagcaac
     3301 tttgtatccc gaaaaatata tttacgatat tcctgggtat ccagcaataa aagcaagtga
     3361 attaatcgat caattagaaa aacaattaac tacttttaac catacattcc atttaaaaga
     3421 agaagttctc tcgcttactc gtgaagacga agttatcgag attactacaa ataaaggcat
     3481 ccattattcc aaagcagtca ttttagcttt aggaaatggt tcattccagc caagaaaatt
     3541 gaatttagat aatgctgaat catttgaaaa ccacggattg gattatttcg tgaatgattt
     3601 gatgaagtat gctggaaaaa aagtcgctat tgctggaggt ggtgattctg ctatcgattg
     3661 gacgttgatg ttagagccta ttgcaagcga agtttacttg attcatcgtc gcccggaatt
     3721 tcgtgcgcac gaacatagtg tatcacgctt gaaatcttca tctgtcaact tgcttactcc
     3781 ctatctcatc gatggattat caggcaataa tggggaactg acagatatcc ggttaaaaaa
     3841 agtgaaaagt gacgagacga ttgatttgat gattgattct ctgatcgtca attatggatt
     3901 ctcttctaat ttagaacatc tttcttcttg gggacttgat agtaccagaa atgccattac
     3961 tgtaaaatca gatatgtcta cttctattcc aggcgtttat gcagctggtg acatctgtac
     4021 ctatgaagga aaagtgaaac tgattgctac tgggctcggt gaagcaccta ctgcagtgaa
     4081 caacgcttta cactatatta atccaaagga acgaactcag cctggtcaca gcacaagctt
     4141 atacgataaa aatcttcgtc ctagttgatt ttttatatct ctttgcattt caagttaaac
     4201 ccgaaagcag tttcttgcat ttttttcatg ttccgttaac atgaaataga gaaagaaaaa
     4261 ataaaggggg agcaataata tggtcgtcaa aactgagact ttagaagaaa aggcaagaga
     4321 actattgaag gaacgtggcg taactgtaga agatatcgca caacttgtca tgtttttaca
     4381 aaaagactat atttctgatt tgactttggc ggattgtact gaaagtgtca acagcgtgct
     4441 aacaaagcgt gaagtacata acgccattat cactggaatc caattagata tattagcaga
     4501 aaagaaaaaa ttgatgagtc cgttgcaaca tattatcgaa gatgacgaag gcctttacgg
     4561 tatcgatgag atcctagcct tgtcaatcgt gaatgtttac ggtacgatcg gctttacgaa
     4621 ttatggttat atcgacaagg tgaaacctgg tattttaaaa gaactaaaca gccacgatgg
     4681 cgttcatgtc cataccttct tagatgatat cgtcggagcg atcgccgctg ctgctgccag
     4741 tcgattagca cacaaagaac cagaaaaaag aagtcgcctt actcaataat tcaacaaaaa
     4801 aagaaagaaa acgtctattt agacgagttc tttctttttt gtttgtttca agctattttt
     4861 tcctaagaaa agaaaaacag caaaaaataa ttctaataat acaaaaaata agataaagct
     4921 atgcatgaaa aattcttctg gaagtacatt gatgattgga tcagtaacgg gatcgaataa
     4981 ccagtcatcg ttgttgaaaa acgtttcatg aaaaaggata aaaaagcgat caaatcctat
     5041 catcatgaaa aaaccgaaaa caactggcag taacatgccg atttgaaacg gacgaatcaa
     5101 ccgccaaagc cgatcattct tttgcaaata tttgataaac aaaatactcg ggatcaaagt
     5161 gatgaaaaac acagcataat ccactaaaaa caaaattttc acttcataaa agtgatgggc
     5221 tccgctagca gaaactggga aatctggcaa gctgaggatg gttttgaagg gattattgag
     5281 ataattcatt aggtgatcaa aatttttcag taacgtctct tgatctaaag aagtataatc
     5341 caaaatttgc aaatgtccga tatcaaatac gtatagtggt cgaaagttga tcgtcacacc
     5401 aactgccaga ctgatcagtg tcagaaatag gctgaacaat ccagcatatt ccatccactg
     5461 ccatctcgaa tttttcatat tagaagctcc attcatccag tgattgtttc acataggtag
     5521 ctgggatagg caacccaggt acatcttctt cttttgtaaa acctgataaa acgagcaaag
     5581 tatctatatt attgcgtatc ccagcttgga tatctgtttc atagttatct ccaaccatga
     5641 ttacttcttc tttttgcagc cctaatactt caacggcttt atccataatg atggcttctg
     5701 gttttccgat gtagatcggc ggcgtttggg ttgctgtctc tacaaaagag atgactgacc
     5761 ctgctccagg aagaaggccg cgttcagtag gtatattctt atctgggttc gttccaatga
     5821 aagtagcacc tttttgaata gcaagtgttg ctttaactac tttttcatag gtcagataat
     5881 tatctaagcc taccactaca tagtcaggag cctcttcttc ccatgtaaat ccggcagcta
     5941 aaatcaagtc aatcaatcct gcttctccga taacatagac ttttttccct tttccatctg
     6001 ctttcataaa atcgattgtt gccaaagtag ctgtgtagac tgtctctgga gctacatgaa
     6061 tgtcaaactc atctgctaga cgattagcta ctgtttctgg tgacttagtc gtgttattgg
     6121 taacaaacaa gaaaggtaat tttctttttt gcaattcttc cacaaatcgt ttaccggccg
     6181 gtatgggttg tgtcccacga taaatcgttc cgtctaaatc aattaaatat cctttatagt
     6241 ccattcatcc actactcttc cctttttctg atcgtaaatg ctgtttttgt tttttctact
     6301 tgtttaggtt gttcttgttt ttttactttt tcagaggttt ttgctgactc tgtttttcga
     6361 tttttaataa ccggtttttt gcgattagat gcgactggtc cttttttctc atcgatatac
     6421 gcacgattat ggtttttttt cttttttcgg cgctgagttg tcttttcttt tttaccgccg
     6481 attctctcga tgacaaaata cgcacaacca aaattgcaat attcgtaaag atagtcttcc
     6541 aaggaatcga ttcgctgatc tggatgcgcc cgtcgattat ccgcttggaa aaatcctttt
     6601 aggcgtagtt gttcataacc ccaatctcct acgatataat catatcgggc tagtacatca
     6661 ctgtaacgtt ctccaagctt ttctgcatta aatccttcac gataatcgga cacaagccga
     6721 taatgtcttt cacctatcaa aaaattactg tcatctaaca gtgtcactag ctcgcctttg
     6781 actttttctg ctttaggctc ctcaacgatt tcttccaaag cagctgtcag ttcttcggtg
     6841 actgttgttt cctgttcagc tgatgatttt ttcttagcaa tttctgtttt atgctcaacg
     6901 ttctgctgtt cattttgttt tgcagacatg cgttcaccac ctatcttttt tattatacct
     6961 tgtttttatt tgattgggta gtgagctttc aagtaatctc ctaccacact tcggataaat
     7021 tcagggaaca aaaattcatt ttttccagca atctcaatcg ttggaaaaaa tggaacaaac
     7081 atcaaatgat ctacggtagt aaaagtataa tttttattca gatcgattgg cttgttcttc
     7141 caaaagactt gatggttaat agaatcatat gtgattccat tatagaccat ctgcccaaaa
     7201 atcttaccgc gaaatcccat tcccttcata ggatagttac gtaaaaagtt acgatttttt
     7261 tctatttcta aaactaagcg gatcagatca tttccaccca atgtgacatt taacaagtgc
     7321 atcggatgcg gcaacgcagt gtgaagctga tcctgattga cccgcccttt tggaatttca
     7381 gttaaaaaca gaccactgtt taatattgct gcttccgtct ttcctcgttc tttcaccgcc
     7441 tcaaaggcta cttggataaa cgaatgctca ttgaagatat ctagactcag agaaaatggc
     7501 aaatctgcta cttctttttc ttttaataat tcatgcccac gttctagata actatttatt
     7561 tcttgttcat cttctgtaaa agctgtcatt gtttcggtag ggatggcttt ggctgaatgc
     7621 ttaaggattt ttttctcttc atcgatggtt aaatggactt caccgacata ctgcccgtac
     7681 tttccagcgg ctgctatttg gacatcattg attacgtgac cttctctgaa taaatgatgg
     7741 gtgtgcgatc ctaaaataac atcaattgct ggtatttctt ttgcaatttg gaaatcatcc
     7801 tgaatcccga gatgactcat caaaactaaa acatccactt gtgaacgtaa atcttcgact
     7861 aattctggca aaatatcata aggattccgt atgtcccagc cattcggatt ataagtaagc
     7921 ggaaatggag cagtaaatgc aattagtccg attttcgtct ggtgtttcgt tgtaatgatt
     7981 ttatatggtt tcgtccatgc cggtggctgg agcgtatttt tatcgaataa gttatccagg
     8041 atgatatcaa actcagcatg atcgtataaa tgattcaact cttctttgga atttcctacg
     8101 ccttcattgt ttcctatcgt cgctgcatca taaccaactt cattcatcag caagatattt
     8161 tcctgtccat tcgttgcttc tgacaatgga tgccaacgat ccacaaaatc acctagatct
     8221 actgcaatcg ttgtcgtttt ttctttcttt tcgttttctc gtttacgttg atccaaaaac
     8281 cttctgatcc ttggccaatt ttctaaatga gaatgcagat cattcgtatg taaaatcact
     8341 attttttcca tgtaccctct cgcctctctt tatctttatc ataccatagg cagctttttc
     8401 ttccattgaa acaagctcta tttcagagtt tttcctgcct tgaagcaaat tgtataagta
     8461 caatattcga taaatcagtc tggctaaaaa atcaggaaca ttcattttta cagtactata
     8521 aaaataaaaa aaacagaccc gaaagtctgt tgtcgtttca ttataatcag acacatggcg
     8581 gaacctgctt agtgctgctt ccttccagac ctgacacgct tcacaagccc accattgtcc
     8641 tagcctttcg gcaaagaata gtatacctta tttttcattc gttgaccagt ctttttttaa
     8701 tttctttttt cgctggcgga gctttcggaa aaagtcagtc aaaatcatcc cacattcgtc
     8761 ttctaaaatg ccaccttcta catatgcttg gtggttaaac cgctcgtcct ccaaaagatt
     8821 catcaaggta ccggctgttc cgccttttgg atcatacgcg ccgaaataaa cttctgctac
     8881 acgagacaaa atcatcgcgc cgctgcacat aggacaaggc tctaaggtaa caaaaagtgc
     8941 agcatcttcc aagcgccagc ttcccacttt ttcacaggct tccttgattg caagcatttc
     9001 tgcatgtgta gtagcatctt gcgtggtttc tctaaggtta tatcctcttc caatcacttt
     9061 tccatccaat acaaccactg ctccaatcgg aacttcatga agcatttctg ctttctttgc
     9121 ttctgccaat gcaaggcgca tccatttttc tttttctact gcatcaagat ttgtatttac
     9181 caattaccct tccctgcctt tgttttttct tgttttactt tagcatatga taaaaaggct
     9241 gtaactaact tttttttcta tggtaaaata aatacaagag gtgaacaaga tgaaaatgga
     9301 taaattactt gctctttacc cacaagcaca gaaaaaaaac tctccagcat cagaagcgga
     9361 tatcctttct tttgcaattg atgatgcttt tatctggatc aaacaagatt cattatctca
     9421 acaagaaacc agccttttga aagcgttatt ccctgtaatc aacgatcaaa aaaaacaccc
     9481 atggtatcgc tatcttttta aagatgcttc ttgtgttcaa gaaaaaagct ttcgtttgat
     9541 ccagcttcac gtcgaatcaa aaggagaatt cttaaaaaaa gaatggcaag atactatttt
     9601 ggaattattt cgctattctg ctgatttctt tttttataca gacacagatg cgctcttagt
     9661 cgaacaaata aaccgtgatt atcttgatgc tactgaaatc aacggtattt ttctatcttt
     9721 agatgctgat tttgatacga caacgtctgt attcgttggt tctttccatt cacctggcac
     9781 cgacctcgtc cctttgtttg ctgaagaaag aaaaattttt ttaacagagc aaaacaatct
     9841 ttatactcac agccgtactt tttctatgca agatgtggct ttacattact atacaaaaga
     9901 tactgtaaaa gatagtaagt tgatcaaaag ttacatagaa ctgatcaaac aaaatgaaat
     9961 ggaagtgatc attcaagaat tatggaatga attaggcaat gtcagctctg ctgcaaaaac
    10021 actgtttctt catcgtaata ctctaaaata tcgaatcgaa aaatttcaag aacagtctgg
    10081 tttcaatcta aaaaaagcca acgatttact gttttgctat cttttattgc ttcatgaaca
    10141 taattaaaga tagattaaag gggttgtgac aataattttg tcacaacccc ttatttcgaa
    10201 tgaatagtgt tcaaaagcta gtttctctta catcattttt tcaacaaaat gcattagccg
    10261 gtcttttaca tcttcggttg aatctttgat tgaatcacca taaacagaga ttttcttttt
    10321 tcctgatttg atttttgcta acattcccat caaagaatcc aagtcactat ctgaaagacc
    10381 atctacgatt tctagtaatt tttcatttcc atcaaatttg tcatagacga atttttttac
    10441 tttcgtccga gtagtcatat gatggatctt ttccattact tttcccgata caacgactgc
    10501 tgtcgctaca ctggcagcgg ctgctacgct taatccgatt gttacttttg ttgatgtttt
    10561 cataagtatc agctcctttt tatctatctc catcatagca tagacaaaaa aataaacgaa
    10621 ctaatccact cagtgaaatc tcaatccttt agggaaaaag ttctttactg ttccttttac
    10681 ttgtttccca aatccgacag gtatcttatc taatactagc aaaacccatc cttgattccc
    10741 atcacgttga aatgtttctc ctgctgtgta agattgccat tccttttgag tgatcggcaa
    10801 gcaaggtata ttctctatct tttttgtagc aagcgctaat gcatagctag gttcaaatcg
    10861 attctttttg aaatctccta aatgcaaacc tgtacgtacg acttttaaac catcgagaga
    10921 aggggctaac tcaggaactt cccaaagatg gtcgttgaac actaaaagtc ttccagtagc
    10981 ttcataatga aaatcatttg aaaactccgt ccagagtttt tcctgctctt ttgtcatttg
    11041 cactttactt ttttttcgtg tcttcttttc tttatgcatc tgattttgtc catgaaaagt
    11101 cagctttgca acaaaatgtc cttcaccctg atctttatga ggccatatac gtattgtttt
    11161 ttccaaacca gctacgcttc cccattcaga acgtcctgaa ctaacggatt gagtcaaagg
    11221 aatctcctca atcgttactg gataattttc tacaagccag ctaatgatct cttcattttc
    11281 ttctggtgca aacgtacaag tcgaataaat cagttgtcct ttatttttta gcatttttat
    11341 tgctgaagac aaaatctctt gctgacgttt ttgacagtat agcggagatt cttctgtcca
    11401 ctcttttatt gcatttggat cttttcggaa cattccttct cctgaacaag gagcatcaac
    11461 cacgatccga tcaaaaaagc ccgaaaaatg agggacaagt tctgcgggtg catgatttgt
    11521 cactattgcg ttcgaaactc cccatcgttc tatattttct gataaaatct tcgcacgttt
    11581 cggaaagatc tcattcgtca ctaataaccc tttccccttc atttgagcag ctaattgggt
    11641 tgatttacca ccgggagccg cacacaaatc caaaactttt tcaccgggtt ttgctgcagc
    11701 agctgttcca acgatcattg cacttggttc ttggctatat tcatacccag cttgatggag
    11761 gaaagacttt ccattgacag tgccaagaaa tccctcattg ctgtagggag cgggttgtaa
    11821 ttcttcgcta tggtaagttt ggaccatgtc caatcctgcc ggttttagtg gattccaccg
    11881 aaatcctttt ttcacgcttc cttgttcaag cgcagaaaaa aaatcggatg cttcttcgcc
    11941 taaaagaaga cgatattttt tgataaattg ctgtggtaac gttgtcgctt cctccttcat
    12001 tccaatgttt cgttagacat tttacaatct tttttacgaa aaataaaggt ggctgatcta
    12061 ctttttcatg ttgctgccta aggtgactgt tcctttaaga tattttccaa gtattcatcc
    12121 gtcacctcgt ttttatccca aacagcgttc atcatttcaa gaaaatcttg agaagaatca
    12181 tctgagcgca aacttcccaa tgcattaagc cataccaatt caagcgactt ttcttctttg
    12241 atcttcgtgt gacctttgac atatgagaaa aatggccgct taaatagcga atccatgtct
    12301 aaatcagatt ttgcaatgat ttcacgaata ctttcatgga tagaaaaaaa atctttcaat
    12361 aagtagacaa tgcttaaaac atcatcggaa ctgcattcag ctaagtagtt ttcaaaataa
    12421 acagtgattt cttctaatat cttctctttt aacccgctga ttgatttgaa atgtgtataa
    12481 atcgggtatg tagaacaacc gcagaactct gctaactctc ttgcagtaat tgacttcatt
    12541 ccatattctt ccacgtatac catacttttg aacagcaaat attctttact ataatttgtt
    12601 actctcccca tcattcttac ctccgtctct tttcatctac ccataaataa ataacataaa
    12661 taattctaaa aatattattg ataattatca aaacatattt agttatatcc attaaaaatc
    12721 aatttttccg ccttaaaaac aagaatatct tgacaaaata ccctaggaaa taaacaatag
    12781 aataaacaag gtaacaaaaa aattaaataa cgttatataa aaaagaaggc gacactctgt
    12841 gagaataaca cagactgaaa tgactttcca ctcacagttt ttgtctaact atatacaaaa
    12901 acagaataaa aaggaggtat ttatgctaat tgaagcaaga aaaaaagagt tacttgttct
    12961 acaaatggtc attcaaaaaa ataattgttc tttacaagaa ttatccaatg acttacatat
    13021 tccaaaacgg acaatcaaag aactgatccg aaaaataaat gtatcactca agcaattttt
    13081 attgattgat gaatttattt attctaatca taaaggagag atccaaacca aagaaaccga
    13141 tcaaactaaa aagttagctg ttttttctaa gttaaaatta tgctacctaa aagaatccaa
    13201 tcgttttaac tatttgatgt tactaatcaa ttatccacaa actgctgtac caaaaaaata
    13261 tttacttgaa caactttata tatcgccctc ctatctcgaa aaactgacaa aacagctcaa
    13321 tcattcctta aaaaaattcc atttacggat cctatctgta aaaaattgct gtactcttga
    13381 aggaaatgag ttatctatcc gtctctatct ttactttttt ctttcagata catttggagg
    13441 taacgattgg ccattttcct cacttgaatt aaatgcacta aaaatgaaca gtacagatga
    13501 gcaaatagaa aaaatttcaa aacaagaaaa aaatatctac ttgctgttaa ctttattcat
    13561 attacgaaac aaacaacagg catattttcc aaaaacttct tctgaaatca ctgaattgat
    13621 ggacttattg cagcatacac atgattattc agaatacttt acttcttttt cagtgagtcg
    13681 cccttgtgaa gagacagaaa cagagagagc ctactttaat tattttttct attgtttttt
    13741 tccaaatcaa atctcttttc gacaaaaaca ggagattggt tttttatttt ccacaagcaa
    13801 aaaccttatt tgtcgacaag tcggtcaatt gatgacttac ttaactcaac ag
//
LOCUS       DACDKL010000063        13571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDKL010000063 DACDKL010000000
VERSION     DACDKL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.063"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            141..1187
                     /gene="gmd"
                     /locus_tag="IV329_001410"
     CDS             141..1187
                     /gene="gmd"
                     /locus_tag="IV329_001410"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP8940599.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1267..2280
                     /locus_tag="IV329_001411"
     CDS             1267..2280
                     /locus_tag="IV329_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940600.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2317..3261
                     /locus_tag="IV329_001412"
     CDS             2317..3261
                     /locus_tag="IV329_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP8940601.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3329..4642
                     /locus_tag="IV329_001413"
     CDS             3329..4642
                     /locus_tag="IV329_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8940602.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4726..5628
                     /locus_tag="IV329_001414"
     CDS             4726..5628
                     /locus_tag="IV329_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP8940603.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5633..7285
                     /locus_tag="IV329_001415"
     CDS             5633..7285
                     /locus_tag="IV329_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8940604.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7309..8175
                     /locus_tag="IV329_001416"
     CDS             7309..8175
                     /locus_tag="IV329_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8940605.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8217..8408)
                     /locus_tag="IV329_001417"
     CDS             complement(8217..8408)
                     /locus_tag="IV329_001417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940606.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8438..8977
                     /locus_tag="IV329_001418"
     CDS             8438..8977
                     /locus_tag="IV329_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940607.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9105..9458
                     /locus_tag="IV329_001419"
     CDS             9105..9458
                     /locus_tag="IV329_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940608.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9926..10402
                     /locus_tag="IV329_001420"
                     /pseudo
     CDS             9926..10402
                     /locus_tag="IV329_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10402..10596
                     /locus_tag="IV329_001421"
     CDS             10402..10596
                     /locus_tag="IV329_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940609.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10602..10887
                     /locus_tag="IV329_001422"
                     /pseudo
     CDS             10602..10887
                     /locus_tag="IV329_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10901..11267
                     /locus_tag="IV329_001423"
                     /pseudo
     CDS             10901..11267
                     /locus_tag="IV329_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            11481..11879
                     /gene="tnpA"
                     /locus_tag="IV329_001424"
                     /pseudo
     CDS             11481..11879
                     /gene="tnpA"
                     /locus_tag="IV329_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12040..12261
                     /locus_tag="IV329_001425"
     CDS             12040..12261
                     /locus_tag="IV329_001425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8940610.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12603..12974)
                     /locus_tag="IV329_001426"
     CDS             complement(12603..12974)
                     /locus_tag="IV329_001426"
                     /inference="COORDINATES: protein motif:HMM:NF025126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940611.1"
                     /translation="MERQVFNRTKTTTHSPLYQFRTTTRDGYKKYVSKSFICESCPLL
                     KNCTESQKHQKEIHRHIWEDYLDEAEHLRHTVYNREKYKKRKETIERIFADAKEKHGM
                     RYTKYRGLEKIRCIQCLFLSQ"
     gene            complement(12842..13366)
                     /locus_tag="IV329_001427"
     CDS             complement(12842..13366)
                     /locus_tag="IV329_001427"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8940612.1"
                     /translation="MDSTHIKANANKRIFTRDMAHKEAILYQKQLEEEINEARIAEGK
                     RPFGSIIRQEVVERKFSIADPESGYYVKTEREKQFAYSAHTVGDENGFVLDVMITPGN
                     LHDSRMLVPQIERVKSRYGVAANAAYKTPWNVKYLIERKLRPILPYISFAQLLEMAIK
                     NMYLNLLFVKVVHY"
     gene            complement(13406..13546)
                     /locus_tag="IV329_001428"
                     /pseudo
     CDS             complement(13406..13546)
                     /locus_tag="IV329_001428"
                     /inference="COORDINATES: protein motif:HMM:NF017416.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3932 a         2686 c         3301 g         3652 t
ORIGIN      
        1 cacgaaaaac taatattgtg ctaatgcgtg cgtagattgt tctatgaaat atcgaaaatt
       61 atcgtgtatt cttgaaaagt attagaaatt acgagccatt tatatggcct tatcacttac
      121 ataggaaagg ataatgtatc atgaaaaaag cgctgattac tggtattact ggtcaggacg
      181 gttcctatct ggcagatttc ctgctagaga agggctacga agttcacggt atcactcgcc
      241 gtgcttctat ttccaatacc gctcgcattg accatttgat gggcaagatc acccttcatg
      301 acggtgacct gtccgactca tcttctctga ttcgtatcat cagcattgtt cagcctgatg
      361 aaatctataa cctcgctgca cagagccatg ttcaggtttc ttttgatgtt cctgagtact
      421 ccggcgatgt tgatgctttg ggcgttatgc gtattctgga agcttgccgt attctgggcc
      481 tgacgaagaa gactaagatt tatcaggcat ctacttctga gctgtatggc aaggttgaag
      541 aagttcctca gcgtgaaacc actccgtttc atccttacag cccttatgct gttgcaaagc
      601 agtatggctt ctggatcact aaggaatatc gtgaagctta tggcatgttc gctgtcaacg
      661 gtattctgtt caaccatgaa tccgaacgtc gcggtgagaa tttcgtcacc cgtaagatca
      721 ccctcgctgc cggccgcatt gcagaaggcc tgcaggacca tctggaactg ggcaacatgg
      781 actccctgcg tgactggggc tatgcaaagg actatgttga gtgcatgtgg atgattctcc
      841 agcatgagac tcccgaggac ttcgtgatcg ctaccggcga gcagcacact gttcgtgact
      901 tcaccgagaa ggcattcgca gcaaatggca tgaccattcg ttgggaaggc actggcattg
      961 atgaaaaggg ctacgatgtt gaaaccggca agatgctcgt ttgtgtaaat ccacagtggt
     1021 tccgtccgac tgacgttgac aacctgtggg gcgacccgac taaggcaaag accgttctcg
     1081 gttggaatcc gcagaagacc tcttacgagg aactggttaa catcatggca aagcatgacc
     1141 gtgaattggc aaagcgcgaa gctgcaatga agaacgcagt caagtaagct gacattgaat
     1201 aaacatatct gtttctatct ttccagtcac ggcttcggac atatcgcgcg gaatctgccc
     1261 attattatgg caacactctg ccgatgcgaa gcgaagatta cactggtttg cggcgcaaat
     1321 catctggatt ttgcaaagcg caatttggag caggaactgc ccaaagagct gatagaccgc
     1381 gtgcgctatc gtccaataaa cacagatatc ggtctgatta accgtgaggg caccctactg
     1441 gtggacacgg gtaagctgac cgagatctgt gaatattttt tggcaaaggt tccttctttg
     1501 gcaaagacag aagccgaatg gctcaagcga gaagctgtca gcgcagtgct ttgcgatatg
     1561 ccaatctggg caatggatgc ctgcgatatg gcaggagtac ctttcctgta tgtaggaaac
     1621 ttcacttgga cagagctgta tcgcgaattt ttacccgaac atatttggaa agaatatgcc
     1681 aagcgttacg gtaaaattca gcacaccatg ctttatgcgc taaataacga agaaatgttg
     1741 gaatttctcc aaaacagcga aaaaacaccg acttccgttg tggcgcgtcc gattcataac
     1801 gacgaagtgc aacgcattaa atcacagcat gaaaaaccgc ctgtatttgt cgcactcggt
     1861 atgtccgcgc ggttcaaaca gccgattgat gtcagtcacg cgccgtatga tttcattaca
     1921 acgctaggtg ttccgcttgt gggagaaaac gtaaccgtta tttctaacag cacccccaat
     1981 acacaggact atatagcggc ggcagattac gttattacaa aggctggatg gggaactgtt
     2041 gcggagtgta ttctcgcaca gcgtccgatg gcactgttcg cccgcgatac agttttggaa
     2101 gatagaacaa ctatttgtaa attgcaaaaa ctgggtctgg caatttccgt cacaccggaa
     2161 aacctccacc gcgtagagcg ggtgattgag gacctcaaac agctagacac ctcaaatttt
     2221 gatacatttt atgatgctgc gagcgaaatc gcagacaaat tgatttctct catgcaatga
     2281 gagatgctct aaatttagaa aggagcaagc attattatga tggaaaaaga cgcaaaaatc
     2341 tacgtagccg gtcatcgtgg catggctggt tccgcaatcg tgcgcgagct gaaccgtcag
     2401 gactacaaca atattatcac ccgcacccat aaggagcttg acctgtgccg tcaggacgct
     2461 gttgaagcat ttttcgcaca ggaaaagccg gattatgtat ttctcgctgc tgcaaaggtt
     2521 ggcggtatta tcgcaaacca gaacgctctg gcagacttca tgtatgaaaa catgattctg
     2581 gaaatgaacg tcatcaactc cgcatgggag aatggctgta agaagttaca gttcctcggt
     2641 tcttcctgta tctatccgcg tatggcaccg cagccgatgc cggaaagctg tctgctgaca
     2701 agtgagctgg aaaagaccaa cgaggcttat gcactggcaa agatttctgg cctgaagtac
     2761 tgtgagtttc taaacaagca gtacggtact gactatatct ctgttatgcc tactaacctg
     2821 tatggaccta acgacaacta tcatcctact cacagtcatg tgctccccgc tttgattcgt
     2881 cgcttccatg aggctaagga agcaggtctg cctactgtta cctgctgggg tgatggttct
     2941 cctctgcgcg aattcctata tgttgacgat ctggcaaacc tgtgtgtgtt cctgatgaat
     3001 aactatagcg gtgacgagac tgttaatgca ggtactggca aggaactgtc tatcaaggaa
     3061 ctgaccgaga tggtagcaaa ggttattggt tacgaaggcg agattctgtg ggataccagc
     3121 aagccgaacg gtacacctcg taagctgctg gatgtttcca aggcaaccaa gctgggatgg
     3181 acatataaga ctgagttgga agatggcatc cgtctgtcct acgaggactt cctgaataac
     3241 ccgatgcgtg ctgaacgcta atttaccgag gttgctgtga tttttgagga ttgcagcagc
     3301 ctctttctta taaaaaggag agatcattat gaatattgtt ttactgtctg gcggttctgg
     3361 taagcgcctt tggccgctgt ctaatgacat ccgttccaag cagttcatta agattttcaa
     3421 aactgaagat ggatatgaat ccatggttca gcgtgtctac cgccagatta agaaggtaga
     3481 taacgacgca actgttacga ttgcaacctc taagacacag gtttctgcta tccataacca
     3541 acttggtgag gatgtgggtg tatctgtaga gccttgtcgc cgcgatacct tcccggcaat
     3601 cgcattggct accgcttatc tggtagatgt gatgggcatt gatccagaag agtccgtagt
     3661 tgtttgcccg gttgaccctt acgtagaaga tgactatttt gaggccctga aatctctgtc
     3721 cgatcaggct gacaaaggcg aagctaatct ggtgctcatg ggtattgaac cgacttatcc
     3781 ttccgagaag tacggctaca tcattcctga gagcgcagag cagactgctt tcgtaaatac
     3841 cttcaaggaa aaaccttctg aggctgtggc aaaggaatat atcgcacagg gtgcactgtg
     3901 gaacggtggt gtgtttgtct acaagctgaa gtatgtcatg gagcgtgcac atcagctgat
     3961 tgactttgta gattatcagg atctgttcaa caaatacgat actctgacta agatttcctt
     4021 cgactatgcc gttgttgagc atgaggacaa gattcaggta cagcgttttg ctggtatgtg
     4081 gaaggatctg gggacttgga atactctgac cgaaggtatg gaggaatcca ttatcggtaa
     4141 aggtgttatg aatgagaatt gcgatggcgt acacatcctg aacgaactgg atgttcctgt
     4201 gttggctatg ggtctgcatg atgtggtcat ctgtgcatct cgggaaggta ttctggtttc
     4261 cgataaggaa cagtccagct atattaagcc tttcgttgac aagttcgagc agcagatcat
     4321 gttcgcagag aagtcttggg gtagttataa ggttatggat gtagaagcag agagcatgac
     4381 cattaaggtt actctgaatg ctagtcactc catgaattat cacagccaca agaaccgtga
     4441 cgaggtgtgg gtggttctgg ccggtactgg taagaccatc gtggatggat atgagcaggt
     4501 tgttaaggct ggcgatgtga tcaccatgag tgctggctgt cggcatacca ttatggcaga
     4561 tactgagctg aagctgattg aagtacagct tggcaaggat atcaatgtta aagataaaca
     4621 gaaatttgag ttagagtatt aaaaaacgga ggagggctcg ttatacagaa accgttttta
     4681 ttgaagcctt ccgcaaagga ttatctgtgg gggggaaccg cctgaatgat gaatttgcaa
     4741 aaggaattga gatgtctcct cttgcggaaa cttggaatgc tctacgcacc cggatggacc
     4801 gagctttgtt gtgtctggtc agagtatcag gggatgacct tgacggaggt gttgcaagaa
     4861 cacccagagt ttctgggaac gcatccgcag accaaaggag aattgccgat cctgatcaaa
     4921 ttcattgatg cgaaaaagga tttgtctgtt caggtacatc ccacagatga atatgcaatt
     4981 gaaaaagaaa acgggcagct tggaaagaca gagatgtggt atgtactgga tgccagctta
     5041 atctatgggc tgcagaaaat aacagaagaa gctgtgatcc gtgaagcgat tgctcaggga
     5101 actgttgagc gttatcttca gaaggttccg gtcaaaaaga atgatgtgtt ctttattgag
     5161 gcgggaacga ttcatgcgat tggtgcgggt gcactaattg cagaaattca ggaaaattcc
     5221 aatctgactt atcgtctcta cgattatgac cgtgttgaca aaaatggtaa aaagagagaa
     5281 cttcatgtag acaaagcgct ggaagtagcg aacttgaaag ggttgtcaga accgaaacag
     5341 ccgcttcgtg ttctaaaata tcgtgctggt gtagctgcgg aattgctttg cagatgcaag
     5401 catttcgagg tctctcgtat gattgtcaac actgagagaa gacagacggt acactatcgt
     5461 gcggattcaa tcagcttccg tgtgttgctg tgtgtcaatg gctgtggtac cgttcgattt
     5521 gataatgaat cgttggacat ctataagggg gactgccttt ttgtgccggc cgattctact
     5581 gaaatcagca ttcacgggtc gattcagttt ctggatgtga gaggataata atatgaatta
     5641 taaagtagaa tatgagcgtt ggctgaatca tgccaatgca gatattatag aagaactgcg
     5701 ttcctattct gagaaggaca ttgaggactc tttctataaa aatttgtctt tcggtactgg
     5761 tggtctgcgt ggtaccatcg gtgcaggcac taatcgcatg aacgtacaca ccgtaggtaa
     5821 ggcaagccaa ggtctggcag actatctgaa taagaactat gaagccgctt ccgtggtggt
     5881 tggctatgat tcccgtatca aatctgatgt gtttgcaaag actgccgcag acatctttgc
     5941 tgcaaatggt attcaggtgc acttgtggcc agaactgctt cctgttccta cagtatcttt
     6001 tgcgactcgt tatctgaagg cagatgccgg tgttatgatt acggcatctc ataacccttc
     6061 taaatataac ggctacaagg tatacggtgc agacggctgt cagattacta ccgaggctgc
     6121 tactgcaatt ctggcagaaa tcgaaaagct ggacatcttt gctgatgtaa agatggatgg
     6181 cgatgcttct aagattgcat ccattcagtc ggaagttctg actgctttca tcgaagaagt
     6241 gaaggggcag tccgtcctgt ttggcgaaga agtggacaag aatgttgcta tcgtgtattc
     6301 tcctctgaat ggtaccggtc tggtgcctgt tacccgcacc ctggcagaga tgggatacag
     6361 caatgtgacc gttgtggaag aacagcgtct gccggacggt aatttcccaa cttgtcctta
     6421 tccgaaccct gaaattaagg aagctatgtc tctgggtatg gagtatgcca agaagtacaa
     6481 tgcggatctg ctgttggcca ctgaccctga ctgcgaccgt gttggtatcg cagttaagag
     6541 cgcacatggt gactatgttc tgttgtctgg taatgagact ggtctgctac ttctggacta
     6601 catctgttct cagagaatta agcatggaaa aatgcctgct gaccctgtga tggtcaagac
     6661 catcgtcacc atggatttgg gtgaacgtat cgcatccaac tatggtgttc gtaccatcga
     6721 tgtgctgacc ggctttaagt ttatcggtga gcagattggt atgttggaaa aggctggtaa
     6781 ggttgacagc tatatcttcg gctttgagga atcttacggc tatctgtccg gtagctatgt
     6841 ccgtgacaag gacggtgtta acgctgctta tctaatctgt gaaatgttct cttactacaa
     6901 gactcagggt atcagcctgc tggataagct gaacgagatt tatacaaagt atggctattg
     6961 tttgaacacc ctgcattcct tcgagtttga cggtgcggca ggttttacca agatgcagga
     7021 aattatggtt gaattccaca agggtctcga tataattggt ggtaagaagg ttattgaaac
     7081 tttagactac agtaagggcc tgaatggtct gccgaagtct gatgttttga agtttatgct
     7141 ggaagataac tgctctgttg tggttcgtcc ttctggtact gagccgaagc tgaagaccta
     7201 tatcagcgtc agcgcagaga acaaggaagc cgccgaagct atggaagcta ttatggctgc
     7261 tgaactggcg gaaatgttcg agtaagaaaa gaaaggagaa atcttgtaat ggaggaactg
     7321 ttttctaacg ctttgaaatc ggtgaagtca aatcgtgtgc tgtacacggc atcgttcttt
     7381 gcaagatcct ctcttttgta tttgcaggaa attggacagc tgactgctct gaaggcacat
     7441 acatctaggc gtacaggatt gtcatcttac ctttgtttcc tggtagaagt tggttcaggc
     7501 acattggagt ataaggggca gaaatatacg cttcaagtag gagatatggt gtttattgac
     7561 tgtttgaagc cgtatgtcca tgagtcatca gaagatgatt tgtggacttt gcggtggtgc
     7621 cattttaatg gcattggtat gacggaagtc tacaaaaaat accttgaacg aggtggtcaa
     7681 ccggtcttta aaacatcaaa aacagataag tatgctgact tgctgaggag ccttcatatg
     7741 gtggcagagg gacaggagta tgtccgggat atgaaaatca atgttctact cagtgaactg
     7801 ctgatgcatt tgatggaaga ttcttggaat cctgacaaag tgccgaattt ctctacaaaa
     7861 aaaatggaat tgcagaacat aaaacaatat ctggatgacc attacacgga gaagattacg
     7921 ctggatcagt tgagtgcaga gttttttatc aataaacagt acttagctaa gatttttcgt
     7981 gaaacttacg gtacaactgt tgtcggttat ctgaatcaga aacgaatcac acaggccaag
     8041 cacttgctac gctttacaaa tctaagtatg gaagagattg caacaaaaat tggcataaca
     8101 gatgcaaact atttttccag aacttttttc aaggtggaag gtgttcgtcc cagcgaattc
     8161 cgcaagatgt ggtaataaaa agccggcggt gttgagccgc cggtcagtac ctgtcgttaa
     8221 cccgcattca aagctttaat ctcagctttg atttcagcac gaacacgata gtaatcttca
     8281 gtttcgattt cggtatccag ccgagttgct gtcttttcgc agtcaggaca acgctctggc
     8341 tgttcgtcgg cagggaagct gtagtggcag gaatcacagg tgtaaatgtg atcgtcacgt
     8401 ataatcatca cctaccaatc cagtgcttag atttttcatg caattactat tatatcgaat
     8461 tggtgattcc acaaaaatcc gcttcttgat aaatgtacga ttcagagtga tatatagtac
     8521 tgtactgcca aagggaatac cacaacggca gaaatggatg attcacatgg aagaaattat
     8581 gcagatgatt ttagggaacg caaaattcca gctcggacag gtggtttcga cccctgctgt
     8641 attggaaact ttcaaccccc tgcagaccgc aaacggcatt cccgaagcct tgagcaacct
     8701 ttggtttaga caggaaatta gtattgcgga gatcgaggac gcagttctgg atgccaagaa
     8761 cggcaaggag ctagcaaaca gcctcaaccg catcaagctt ttccgcaagt tcaccctcga
     8821 ccgagagctg gacggcatgg tcagactgaa gggcacggac gtctggaaga acatcagcta
     8881 cctcaaggtc tacaccgcca cgccgcctaa gaaaccgacc cccccaaggt tccagtcgaa
     8941 cggctggagc cttttcttct ctcataaatc ggcataatgt atacaaatac agctccaaat
     9001 gattgtgtag tatatgcctg tggattgact tgatatagtc gagagactat ggcaatatac
     9061 acacaaccaa aggggaaaat cccaaaaaca aggaggaaac gctaatggca atgagcgaaa
     9121 aggcaaagaa atatttgaag gagatcaagg gtgcaaagac catcccggag ctgaaggatg
     9181 tggagatcgc aatcaagcga gacggtatcc ttgcttgggc agagttcacc aagttgaacg
     9241 aggcggtgga agagaagaag gtcgcccttc gcaagaaaaa gcaggagacc agccttcagg
     9301 agatcctttt ctgggcctac aaaaaggaat cagacaagct gctgaagatg atggacgagg
     9361 gagcagacaa ggatgccatc cagatgcagg tccagcgtta cgacagcatc ggccagatca
     9421 ttgcagaggc agacctcgaa gatgagtatg aggtttgaga atacagcctg aaccactaag
     9481 aaccaaatac cgaaagggac ttctgaaaca gacagaggtc cctttctttt gctcactttg
     9541 gcggtcaatt atgaaccaaa aaccagtcga atgattgtgt acattatggc tttcaaacga
     9601 cttgataata atcactttta gagtgatata tgtactacaa aaaaacaaaa gactggagaa
     9661 aacaacagat gagcgaatac agaatcattt atatcagcga cgaggacggc ggcgaggtgg
     9721 aaggatgtgg ctacacggct acgatggtta tgatgcggtc agaaacttct tggaatttgc
     9781 ctgcggcatc ttgaaaatcc tgcgggtgcg attcatcaga agagtggact gaaatacaag
     9841 ttagcagata gagaagagag tcaggtttcg acttggctct gttctctgtc tactggcaga
     9901 aatacattga agggaggtga cgactgtggc aaccagagga agaaaatcaa ccccgacagc
     9961 catgaagaag ctggagggaa atccgggcaa tcgcccactc aatgcaaaag agtcaacgcc
    10021 ggaaaagaag gcaccgacct gtccgaagtg gctggaacca gaggcaaaga aggaatggaa
    10081 gcgtctggca aagcagatgg aggccatcgg aatcttgaca gatgtggata tggctgcctt
    10141 tgccggttag tgccaagcct acgcccgctg gaaggaagcc gaggaattca ttacccagca
    10201 cggtatcatc gtgaaaaccc tatccggcta ttggcagcag gtaccacagg tgtttattgc
    10261 ccagacctat ctgaaaatta tgaaccgatt ttccgagcag ttcggtctga cgccttcgtc
    10321 cagaagccgt atcattgccg gtgatggcgg tggtgtcgga gcagaagatg cgatggaggc
    10381 tctgttggga ggtggtagct gatggcagaa accagaccaa agggctatcc caaattaaag
    10441 gaatacacac caagccgatt catgctttcg gaatgtcact acgacaaagc cagagcggat
    10501 cgggcggtga actttatcgg acagctccga cacaccaaag gcaaatgggc aggcaaccgc
    10561 ttctggctct tgccttggca ggaacagatc atctagtgta atctcgctta actgtattaa
    10621 cggacgtagc gactttatta agagtgttca tggtaggagc attagttgca acaatgattg
    10681 atattaaatt tttatggtac caacgattgg cgatgtgaca ttgaatatgc aaaactatat
    10741 cgatatgata ttatctaaaa tcttactcgc tgggatcgtc gaggtgttta ttggttgtta
    10801 ggaaagaaaa atatgaccca actcgagcga tttttatcgt attacttgtg tgtggcttta
    10861 tctgcattgt gtgtgatttc gaaataaggg agagccaaaa atgagtagaa aattgatatt
    10921 gattagtcac ggaaaatttt gtgaggaatt gaaaaaaagt acaaaaatga ttatgggacc
    10981 tcaagagaat atctacacgt tacctttatt accagaagat ggcactgaaa catacagaca
    11041 aaagctctta ctgtcataga ttcgttaaat gattttgtgg tactatgcga tcttttaggc
    11101 ggtacaccta ctaatatcat ttctaaactg ataatggaag gacaacagat taaactttat
    11161 gtcggattaa tcttcctatg gtcattgaat ttatcaactc gcaagtgatt ggttcagagc
    11221 ccgagtttgt tcgtgcagga tgtgaaagca ttgtatctgt caactaatta attagtggaa
    11281 tagtagaaga tgaagagtaa taaaaagaga gtgggacaat agtcagaaaa aaatctgatt
    11341 attgccccac tttttttatt gactttagtc agtttcgttg gcgtagcgta cgaaacaaaa
    11401 aaacgcgcgt ttctacgcgt ggacgaaata gctttagcga taaaagttta gcacatacga
    11461 gatgggattg taagtatcac atagtcttta ccccgaagta tcgacgaaaa gtgatttatg
    11521 gaaagttaag agtaagtatt ggccagatat taagaaaatt gtgtgagagg aaagatgttg
    11581 aaatcattga agctcatgca atgcctgatc acattcatat gttagtaaga ataccgccaa
    11641 agattagtat ttctagtttt atgggctact taaaaggacg aagtgcaacc ttaatccatg
    11701 aaaaacatgc aaatttgaaa tataagtatg ggaataaaag cttttggacc aaaggatact
    11761 acgtgagtac ggttgggtta aatcaaaaaa caattgaaat atatatttga gaacaagaag
    11821 cagatgatcg aattagagac agtataagta aacaagagta tgtagatcca tttaaatagt
    11881 taaagagaga tagggctgtg agtggtgagc ccttctaggg ctgttaaaaa gccacccgtt
    11941 ttcacggatg gatatttact gattttaagg tacacaaaaa gcactcatcc ctgtaggatt
    12001 aaagtgacca aacccaatca gaaaggaagc gagtgcttta tgtttaaaaa ttataccatc
    12061 aaccaaaaaa ttttaccatt agatatggaa caatatattt ctgaaacaga tgtcgctttt
    12121 gcggtcaacg ctttagtcga agaaaagata aaagtggatt tatacgtgtc ttcaaaattt
    12181 acgaatgtga aagtggtcgg tgctgacctg ttcgtagtca atgtacgaaa gcaaaaagtg
    12241 agcgaaaccg acaaatttta gtgaataata catggcgtta tttcaaagca gaatgtaaaa
    12301 aaacttttag aggaaaaaga aggttcgatc tatcggaaac ggatttccac ttatgaagga
    12361 aacaaggagc caagattgac attggcttgg cactcatggc gttaaattaa gaaaattagg
    12421 aaaatacatg gagaggaaaa gacgtaaaga agaaaaaact attgtcccac tctctaagac
    12481 tgtagacaaa agcatcagtt tcctctttac taagagtgta gactgatact tttgtctatt
    12541 tttgtataat aaaagatata acgacatatg ctcccactgc caagtagcta attttttcag
    12601 attcattgcg acaaaaacaa gcattgtatg cacctgattt tttccaagcc tcgatacttg
    12661 gtgtagcgca ttccatgttt ttcctttgcg tctgcaaaaa tgcgttcaat cgtttctttt
    12721 ctttttttat atttttcacg attgtacacc gtatggcgta aatgttctgc ttcatctaag
    12781 tagtcttccc aaatatgtcg atgtatttct ttttggtgtt tttggctttc tgtacaattt
    12841 ttcaataatg gacaactttc acaaataaaa gatttagata catatttttt atagccatct
    12901 cgagtagttg tgcgaaactg atataaggga gaatgggtcg tagttttcgt tcgattaaat
    12961 acttgacgtt ccaaggtgtt ttgtacgcag cattagcagc tacaccataa cgtgatttaa
    13021 cgcgttcgat ttgtggcacg agcattcggc tatcatgtag atttccaggc gtaatcatga
    13081 catctaatac aaaaccattt tcatcaccaa cggtatgagc agaataagca aactgttttt
    13141 ctcgttctgt ttttacataa taaccacttt ctggatctgc gatgctaaat tttctttcga
    13201 ccacctcctg acgaatgatt gaaccaaaag gacgtttgcc ttcggcaatt cttgcctcat
    13261 tgatttcttc ttctaattgt ttttggtata aaatggcttc tttatgtgcc atatctcttg
    13321 taaaaatacg tttgttcgca ttcgctttaa tatgagttga atccatatac aaaatttctt
    13381 catgaataaa tctagcatca actgcttatt cgagaataat gataaatatc tgttcaaaca
    13441 aatcggtttc tctgaaacgc cgtgcatagt ttttaccaaa agttgaaaaa tgaggaactt
    13501 tatcaattaa actaagccca agaaaccaac gataagctag gtttgtttca acttatttaa
    13561 tggtctgacg c
//
LOCUS       DACDKL010000064        13397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDKL010000064 DACDKL010000000
VERSION     DACDKL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.064"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IV329_001429"
     CDS             complement(14..706)
                     /locus_tag="IV329_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8940613.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IV329_001430"
                     /pseudo
     CDS             791..967
                     /locus_tag="IV329_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IV329_001431"
     CDS             complement(1143..1712)
                     /locus_tag="IV329_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8940614.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IV329_001432"
     CDS             complement(1786..2109)
                     /locus_tag="IV329_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940615.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IV329_001433"
     CDS             complement(2263..3075)
                     /locus_tag="IV329_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940616.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IV329_001434"
     CDS             3429..4277
                     /locus_tag="IV329_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8940617.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IV329_001435"
     CDS             4422..5363
                     /locus_tag="IV329_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8940618.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IV329_001436"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IV329_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV329_001437"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IV329_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8940619.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IV329_001438"
     CDS             complement(9827..12013)
                     /locus_tag="IV329_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8940620.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV329_001439"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IV329_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8940621.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IV329_001440"
     CDS             complement(12235..12675)
                     /locus_tag="IV329_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8940622.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IV329_001441"
     CDS             complement(12749..13288)
                     /locus_tag="IV329_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8940623.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3764 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaata
//
LOCUS       DACDKL010000065        13282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDKL010000065 DACDKL010000000
VERSION     DACDKL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.065"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            171..1910
                     /gene="yfcC"
                     /locus_tag="IV329_001442"
     CDS             171..1910
                     /gene="yfcC"
                     /locus_tag="IV329_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8940624.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2067..3251
                     /gene="iadA"
                     /locus_tag="IV329_001443"
     CDS             2067..3251
                     /gene="iadA"
                     /locus_tag="IV329_001443"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8940625.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3362..3565
                     /locus_tag="IV329_001444"
     CDS             3362..3565
                     /locus_tag="IV329_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8940626.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3666..4328)
                     /locus_tag="IV329_001445"
     CDS             complement(3666..4328)
                     /locus_tag="IV329_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940627.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4341..5843)
                     /locus_tag="IV329_001446"
     CDS             complement(4341..5843)
                     /locus_tag="IV329_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940628.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6391..8070
                     /locus_tag="IV329_001447"
     CDS             6391..8070
                     /locus_tag="IV329_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8940629.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8197..8676
                     /locus_tag="IV329_001448"
     CDS             8197..8676
                     /locus_tag="IV329_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8940630.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8895..9575
                     /locus_tag="IV329_001449"
     CDS             8895..9575
                     /locus_tag="IV329_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8940631.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9583..10914
                     /locus_tag="IV329_001450"
     CDS             9583..10914
                     /locus_tag="IV329_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8940632.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10929..11891
                     /locus_tag="IV329_001451"
     CDS             10929..11891
                     /locus_tag="IV329_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8940633.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12002..12340
                     /locus_tag="IV329_001452"
     CDS             12002..12340
                     /locus_tag="IV329_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8940634.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12419..13126
                     /locus_tag="IV329_001453"
     CDS             12419..13126
                     /locus_tag="IV329_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8940635.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4237 a         2338 c         2911 g         3796 t
ORIGIN      
        1 taaatggaaa gagaaaaaaa taatcataca taggagaaaa aaaacaaaat agaaaaaagt
       61 gattttctaa taaattattc atgtggaaat gtcattttta ctgtataatt catttgatta
      121 tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat ttgaagaaaa
      181 caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc catacgtatg
      241 tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt ccagcaggaa
      301 agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt actcgtactg
      361 ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat gtgtttgatc
      421 aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt ccggaaaaag
      481 gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa
      541 tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat gatacttcta
      601 aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc tttggtttct
      661 taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct gtagggatcg
      721 ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg
      781 acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta gctttaccgc
      841 tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa gaggtaattc
      901 cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg atcgtagcag
      961 taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg agcccgttta
     1021 gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg acattccgat
     1081 tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata tatgcagaaa
     1141 aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat ttccgttctc
     1201 atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa ttgatcttat
     1261 tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa ggctattaca
     1321 ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc attgctgttc
     1381 ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa tctggtgcag
     1441 ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt cttgtcttgg
     1501 gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg atcgggacgg
     1561 cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc caaagtttgt
     1621 tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca atcatggctc
     1681 cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat cagcttggtt
     1741 caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg ctaggtgttg
     1801 cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg
     1861 ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag tactggagac
     1921 ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt cattttacgg
     1981 taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc tatcatcagt
     2041 tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa gtgtttgcac
     2101 cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg gctgtagaag
     2161 aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt gagggaaaag
     2221 tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga ggagaaggcg
     2281 gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct ggtgtaacga
     2341 cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg ttgatcagca
     2401 aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt tcgtaccgat
     2461 tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt gataaaatta
     2521 tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt tttgaagagt
     2581 tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa gctgggatca
     2641 tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga atcgtagaag
     2701 aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca ccagaattat
     2761 ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact gctagcgaag
     2821 atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca ctgaaacgat
     2881 tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga caaggaagct
     2941 tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt gccaaggcat
     3001 tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa atcgccttac
     3061 gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa
     3121 ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt gatacagtta
     3181 tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg actttcgaaa
     3241 aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc tgtttgtctg
     3301 tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag gaggaagcac
     3361 aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa
     3421 agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga aagcacaatc
     3481 actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca aagacaagtt
     3541 ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca agcaaataaa
     3601 aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga tcattatcct
     3661 gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac ttcatcacag
     3721 ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg ttcatgggcg
     3781 atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt tccagtaggc
     3841 tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc gactcgctgc
     3901 tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc aattcctaca
     3961 cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt agtgatttcc
     4021 attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta agcttggaaa
     4081 ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag tgaacgatct
     4141 tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga aagaaaagtc
     4201 gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc gaaattcagt
     4261 tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc
     4321 cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa tcttaatatc
     4381 cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc ggctaatagt
     4441 cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga aacagcaaat
     4501 ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg tctgttacct
     4561 ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca
     4621 agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc aactcctaat
     4681 gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt acttctggat
     4741 tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt aatcataatg
     4801 atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt tttagaaaaa
     4861 cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt tgattgtaag
     4921 ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc cattttatct
     4981 ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt cccatttagt
     5041 tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa gttgaatttc
     5101 atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt catttcataa
     5161 gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc caggtcattt
     5221 gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt
     5281 ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg tgcagttgct
     5341 gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc cattcctcca
     5401 gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc agaactagtg
     5461 atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga ataactcttt
     5521 acattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt aagagaaaaa
     5581 ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt
     5641 ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc tttttgtgct
     5701 tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac aaaaataaga
     5761 atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt tgtacttttc
     5821 catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat ttgataatag
     5881 acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca acatgaggtt
     5941 ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat
     6001 gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct agatgctaca
     6061 gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat ttagtttatt
     6121 tgttgtataa taatggggaa cagctgaata cttatataag tattctggct atagcgtttc
     6181 gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc caactgtcag
     6241 aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa tagtgatttt
     6301 gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg acatctttaa
     6361 acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat cccattaggc
     6421 ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga tatttttatt
     6481 ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct
     6541 gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt aacacatgga
     6601 catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt accagtattc
     6661 gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa
     6721 ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg tgaaacagta
     6781 gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa tctgaaaaca
     6841 aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc tatcccaatg
     6901 tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt agccttacta
     6961 agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca aatagcagat
     7021 gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt
     7081 aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg aaaagttgtc
     7141 ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga aaaacttcaa
     7201 ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga tccagaagaa
     7261 ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca aaaaatggca
     7321 aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat cactactacg
     7381 ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta tcgagctggt
     7441 ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa tcctaatgac
     7501 ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca aggagagtat
     7561 cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata caaaaatatc
     7621 tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag tgttgcagga
     7681 actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat cggaaacatt
     7741 gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt tgtcactatc
     7801 agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg attcgtttac
     7861 gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac aatcgttgag
     7921 aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat ccgagatcaa
     7981 ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc agtcattatg
     8041 gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac ctctctatag
     8101 agtgctcgtc ttgctctttt tttgcgaaaa acaaataatt tttgaataca gcatccctcg
     8161 ctttataata aacgatagaa taaagggagt gaatcaatga tgagtcaaac aaaagtcgaa
     8221 cgttatttag aagaacaaaa gatcagctat caaccacttg attttagtca tgtggtagaa
     8281 gaatctttca cagaagaact acaaaaaaga gggattgatc cagctttgat atgtaaaact
     8341 ctcgtagtga aaggaaacaa aactggggtg atcatcgcat tgattccttt gaacgatcac
     8401 ttagattaca aaaaaacgag acaagtgacg aaagatcgta aagttggttt tcctggaatg
     8461 gattttgtat taatgcatac aggatatccc cacggagcaa acacccctat tggaataaaa
     8521 ctcgcacatc ctgattattg ttttttagca gacagttctt taaaagaaaa agaagaagta
     8581 attgtgtcaa gcggtgaaat cgggagaagc cttcggataa aagtagccga tttagaaaag
     8641 atcgtccatc ctatttacgc tgatttaatt agttaaggga cgagaagaga agctaagcag
     8701 agcagagacc tcaaacttga gattatatct gcttggtact tggtgcatcc tgtattatct
     8761 gttacaattg ctttgtacat actgtactat caatccaatg aaaatatatt tagcagctat
     8821 attttttcat tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata cagagaaaac
     8881 gcgaggtgta aaatatgaaa attacgtatc atggacattc ctgtttaaca attgaaatgg
     8941 aaaatggcca acgcgtgatg attgacccgt ttattacagg aaatccattg acagacttaa
     9001 ctgcagaaga agtaaagaca gattggttgc tgattactca tggtcatagt gaccacatag
     9061 gagatatgat tcctattgca aaagaaaatc aagccacaat tatcagtatt gttgagattg
     9121 ctgattatgc gaacagcaga ggagtagatt cattcggtat gaatatcggt ggaaaacatg
     9181 cgtttccatt cggcgtggta aaattcgtac atgcacagca tagttcaagc tatgaagtag
     9241 acggtatcgt tcagtatatg ggcgagccta gcggaattat tattcaagca gaaggaaaaa
     9301 cgatttatca tgcaggggat actgcttatt tcagtgatct aggattgctt gcagaagaat
     9361 tcgatattga tgtcgctttc ttgccgattg gcgataacta tacgatgggg ccagaagatg
     9421 cagcaaaggc tgcgcaaaca attcgtgcca agaaagtagt tccaatccat tacaatactt
     9481 tccctgtcat tgaacaagat cccgaatctt ttgtagagtt actggaagat agtgaaggaa
     9541 aaataatgag cgtctcggaa acgctggaag tgtaggcaga aaatggcgac aaaacatgat
     9601 ttgattttag aatatattga aagtttacct gttggcaatc gaatttctgt aagaagcatc
     9661 gcaaaagaat tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga tgcagaaaat
     9721 gtcggactag tttcaacaat tcaacgagta gggaccatcc gtatcgaacg caaattaaaa
     9781 aaacatattg aacgtttgac atttggcgag gtcgtcagga tcattgaagg ggacgtttta
     9841 ggcggggcgg caggattaga caaggtcttg aataaattcg tcatcggcgc gatgactaaa
     9901 catgccatgg aacgctatat tacgccagga tcactaatga tcgtcggcaa ccgggcggaa
     9961 gtccaaaaat tagcattgga agatggcgcc gcagtattga tcacaggagg attcaatacg
    10021 actgctgaga ttgcaaagct agctgatgat cttgaaatgc cgatcttacg cacctcttat
    10081 gatacattta cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt gatcaaaaaa
    10141 gatatcatgc ttgtcagtga tatctatatg ccagctgaaa agactcatta tttgcagcag
    10201 acagatacga tttctgatta tcagcgtttg tcagaagaaa cgcagcattc gcgttttcca
    10261 gtcgtcaacc gccatcatcg tttgatgggg attgttacgg caaaagatgt tttagggaaa
    10321 tcgccaaacc agatcatcga tcgggtgatg acgaaagaac caatcagtgt aaagaaaaca
    10381 atgagtattg cctctgttag ccatcaaatg atctgggatg ggctagaagt catgccggtc
    10441 gtttccgatg acttgacatt ggaaggcttg attacccggc aagatgtaat gaaagcgatg
    10501 cagcttgtcc agcgacaacc gcagattgca gatacgattt cggatcagat atctggaaat
    10561 atccatacga ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa gttcaaattc
    10621 gtagtagcac cacaaatggt caatggcgtt ggaaccattt cttttggcgt acttagcgaa
    10681 atcatttctg atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa tatcttgatt
    10741 gaacaagtga acctacatta tttgcggctg atccaattgg aaagcgaatt agacattcgt
    10801 ccacgtgtat tggaaatcgg cagacgttcc gctaaactgg atatcgaagt gtttattgaa
    10861 aacaccatcg tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc ttagaaagca
    10921 ggagtgaaat gacagtacag caagaaatag tagatacaat aaaaaaatac gataaaatca
    10981 ttatccacag gcatttgcgc cctgatcctg atgcattagg atcacaagta ggattagccg
    11041 aactattacg ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa acgattgaag
    11101 ggcttcgctt tttagcagaa atgcaagaag ttgaagatgt cttctaccaa ggagcgctag
    11161 tcatcgtgac tgatacagct aattcaccca gaatcagtga tcaaagatat cagttggcag
    11221 aaaaactgat caaaatcgac catcatccca atgatgaccc ttatggagat cttgtgtggg
    11281 taaatacaaa agcaagtagc tgtagtgaga tgattgcaga atttgctttg atgtttcctg
    11341 aatctttaaa gatgaacgtt aaggctgcac ggttacttta tgcaggaatc gtcggagata
    11401 cagggcgctt tttgtatccc tccacgacgt cccgtacatt agagattgct tcgatattga
    11461 gaaattatcc atttgatgca agtgctttga acagagagat tgaacaaatg ccgatgaaag
    11521 tcgctaaact ttccggttat ctttatcaaa atatccaggt ggatgaaaat ggtgcaggaa
    11581 gaataatcct tccgcaagaa attcttgata aatatggaat cgatgattca gagacagctt
    11641 cgatcgtttc acttcctgga gtgattgacg aagtgctggc ttgggggatt tttgttcagc
    11701 agccttcagg gtattaccgc gtacgtttac gttctaaggg gccgatcatc aatgaattgg
    11761 ctaaacgcca tcatggagga ggccatccat tagcaagtgg tgcgaatgca aaagatttag
    11821 cagaagtaga ggaaatctat aaagagatcc aagatatatg ccaatcgtat aagagcagaa
    11881 aaacagaatg atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac aaatgtatct
    11941 tttcaagatc ttctttttcc gttacaatag tgctcactaa agattttaaa ggagaaagaa
    12001 gatgacatta ccaaattgcc cagaatgtgg ctcagagtac acatatgaag atcgaggctt
    12061 gttgatttgc ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa ctgtcataga
    12121 agacggatta gtggtattag atgcaaatgg aaaccgtctg ttagagggcg atagcgtgac
    12181 agtgattaaa gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag gaacaaaagt
    12241 caagaacatc cgtttagtag aaggcgatca taatatcgat tgcaaggtag aaggatttgg
    12301 accgatgaaa ctgaaatcgg aatttgtgaa aaagaattaa agaatattca aggtcttttc
    12361 acattctttt cctactacta atgataaaat aaagtaaacg aaaaggagtg aaacgaaaat
    12421 gtatggttat ggaatgtatg gttttttaga tccgacattt cttttggtca ttcttggatt
    12481 agttatttcc ggaattgcgt cagcgtacgt caatagtact ttccgaaaat acgatcaagt
    12541 gagaagcaaa aacaatgtga ctggtacaca agcagcacaa tatatattac aaagtcaaca
    12601 gataaataat gtcggtgttc aacaaattgc cggagatctc acagataact acaattcagg
    12661 taataagatg ttgagtttgt ctcaagcaac tgcacagtct acttcagttg ctgcgatagg
    12721 tgtagcagct catgaatgcg gacatgcggt acaagatgct tcgggttatg tcccattgaa
    12781 gattcgtgca gcacttgttc cagcagctaa ctttggttcg atgatttcgt tccccttgat
    12841 tatggtaggt gtattgctta gctggaactc tacgttaatc aatatcggga ttttcgcttt
    12901 ttctttagca ttacttttcc aattggtgac attaccagtc gaatttaacg cgtctcggcg
    12961 agcaatccaa attttgagtg aaggcggact actgacggaa gaagaagtac cgatggcaag
    13021 acacgtatta tttgcagcag cactgactta tgttgcagca gcgctttcga cattcctgca
    13081 gctgttgcgc ttgatcttat tgttcggcgg caaccgaaga gattaaaata aagaagttgt
    13141 gaaatcagcg ctttgatctg agctaatacc gagagttagc tgattgaata ccatttattt
    13201 ggaataaggg ctctttgtca ataaggactg atgggttgta caaaatcaaa tctgggtcag
    13261 aatgaacctc attctggctc ag
//
LOCUS       DACDKL010000066        13280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDKL010000066 DACDKL010000000
VERSION     DACDKL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.066"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(11..1534)
                     /locus_tag="IV329_001454"
     CDS             complement(11..1534)
                     /locus_tag="IV329_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8940636.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1786..2967
                     /locus_tag="IV329_001455"
     CDS             1786..2967
                     /locus_tag="IV329_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8940637.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3027..4655)
                     /gene="treC"
                     /locus_tag="IV329_001456"
     CDS             complement(3027..4655)
                     /gene="treC"
                     /locus_tag="IV329_001456"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8940638.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5052..7763)
                     /locus_tag="IV329_001457"
     CDS             complement(5052..7763)
                     /locus_tag="IV329_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8940639.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7773..8474)
                     /locus_tag="IV329_001458"
     CDS             complement(7773..8474)
                     /locus_tag="IV329_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940640.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8660..9259
                     /locus_tag="IV329_001459"
     CDS             8660..9259
                     /locus_tag="IV329_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8940641.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9340..10353
                     /locus_tag="IV329_001460"
     CDS             9340..10353
                     /locus_tag="IV329_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8940642.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10461..11378
                     /gene="rbsK"
                     /locus_tag="IV329_001461"
     CDS             10461..11378
                     /gene="rbsK"
                     /locus_tag="IV329_001461"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8940643.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11406..11801
                     /gene="rbsD"
                     /locus_tag="IV329_001462"
     CDS             11406..11801
                     /gene="rbsD"
                     /locus_tag="IV329_001462"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8940644.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11801..13174
                     /locus_tag="IV329_001463"
     CDS             11801..13174
                     /locus_tag="IV329_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8940645.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         3772 a         2632 c         2458 g         4418 t
ORIGIN      
        1 tttttgccta tcagtgcaaa agcatttcct gtgcaatttc acgtccatcc attgtatctg
       61 gataaaatgt tggccaattc tccatttctt tcagcaatac ttcttgactt tcatttcctg
      121 catataaatg aaaatgttcg gagtcagccg gtgcaatcgt gtgatcactg aattgaatat
      181 atctaaaagc gccactttcc ttatcttctg cttcgaatag ataccttacg ccacggtttc
      241 ctgcctcgta ggtcaagatt tctttcccta cataacgata atttgaacgt ttcgttgttc
      301 catccttaaa cgtaaatgcc atctctttct cactgatctt gattgaagca atatctgttt
      361 gatagccctt cgtataatat tctttatatt cttgtgctgt cttatcctga ttcaattttt
      421 ctttatattc aaaaacagga tcaaaagtcc catcttctac atacgtatct actgtttgcc
      481 aagttcctgc ccaatctgaa agagagcgat cttgtacatc ctcgtcttca aaataaccgt
      541 tgtagacgct cttttctttt ttgggggtca aagtatcttc tagcgggttt tctgtctctg
      601 tcgttttttt caacgcttcg aggtttgctt ccatgataga aagatagttt tctccatttt
      661 ctatttgttc attcgtcaat ccttccaaag gattcaatac ttctaaagat acacctgctt
      721 cgtttgctaa cgttctggca atactatctt tggcgttttc ttcaaaataa atatagttga
      781 ttccatgttc tttaacaaag tgtttcagtt cagcaattct cgccgcagac ggttcttcac
      841 tagaagaaag accagcaatc gatacttgat tcaatccata ttctaacgct agatagttga
      901 atgcagcatg ttgcgtgaca aaagatcttt gttttgcttg cttaaaggtt tcttgataaa
      961 gctgatggag tgcttgaagt ttctcggtat aggctgctgc attttttgtc catacttcct
     1021 gtttttctgg gtaagcttca atcagctgat cacgtatgtt tgctacctgc ttgattgcta
     1081 aacttggagc tagccataca tgtgggtcta gctcatgtgt atggccttct tccccgtgtt
     1141 catggtcatg atcatgtcct tcttcacttc ccggaagtaa aagcatacct tctgtcgctt
     1201 ttatcgtatg gacattgcct tcttgtaatg ttttttggat tgcggggacc catgtttcca
     1261 tgttttcatc attataaaca aagatatctg tatcttggat ctttgccatg tctttagcag
     1321 aaggttcata atcgtgggaa tcagtccctg ctggaatcaa taactctact tctccttcat
     1381 ccccgacgat tgcttttgta aaatcataca tgggataaaa ggtcgtcatc acgctcaatt
     1441 tttctttact gtcctcggct ttcccacttg ccccgcaagc agttaacata cctgctaaaa
     1501 ccagcatggc caatatacct atcaatcgtt tcatttatga cactcctctt ctcatcttct
     1561 ttcatttgaa tgttccgatt cgcttttcgc aaaccgtaat agttacgttt tagcaacagg
     1621 atttaattta tcatatggtt aaaagaactg tcaagcgttt tcttatttta attgatgaga
     1681 aagtttatat gaatgattga cgatattttt gcaatcaacc tctatcattt tccttttata
     1741 cccgcttttg tctataatga agttatagat aaaggaggaa aaagagtgaa aaaagaaaca
     1801 gatacaacga atcgtttgat ccgcttttta ggaggaaaaa cctcttacta tgttttagga
     1861 atagttatct tacttgcgct gatcatttat tttttccatc agatctcgtt tatattcaag
     1921 cctgtcggaa tcattatcgc tactatcttg cccccattat tgtttgcact gatcttgtat
     1981 tatcttttgg aaccagtcat caaacgtttg agcaagcacc tttcgaggaa cttgtcagtc
     2041 attatcgttt atgtgattat tttattttta ttggggcttg gaagcatatg gcttattcct
     2101 tttcttgaag aacaggcaaa gacattgatc agtagtttgc cagatttgtt tcaagatttt
     2161 caagagagtc tccgacaatt tttggagaaa acaccttttg ccgattcatt taatcaattt
     2221 tttgcttcta ttgacgacgt gacgtctgat atagctggat ttattggaaa ttattgggaa
     2281 agtggcgcac aacggatcag caacattttt tcaactgtta cagctatttt tattacttta
     2341 tttactggtc caattgtcgc ttttttcctt ttgagagatc ctcaaaaatt ttatcagtca
     2401 gttttagcga ttgtcccacc agcatttcgt acagatttta aaaatttgac aaagattgct
     2461 aatcaacaat tgggttcatt tctaaagggg caaatcattg cttcatttat ccttggagca
     2521 gtctactggg tttgtttctt actgatcgga ttagaatttg ctagtgtttt agctatttct
     2581 gctgggttgt tatgtatcat tccatatatt gggccgttta tcgccttctt tccaggtttg
     2641 atcattgcat tccaagactc tacttttatg gctgtcaaat ttgtcattgt ctggtttgcc
     2701 gtacaacttc tccacggtga cttggtgatt cctcgagtaa tgggtgatcg tttacagatc
     2761 catccgatta cgatcttaat cgttttactt gtcatgggtg atctaatggg aattgtgggc
     2821 gttatttttg gtattcctat ctatacgtta gtcaaattac tcgttacttt tattttccgt
     2881 aaattcaaac aacgctacaa taaattttat ggcgataaag gagagtatga aaactcttca
     2941 ttttctgaag aagattattt taaataaaac acaaaacgtc gatcttacga gaatagctcg
     3001 taggaacgac gttttgtgtt tttgttttaa tttttcagca aagcaattgc ctggtatggt
     3061 tttagctcca gactttgatg aagaacagta tcggaataat tatcgataag aatattggct
     3121 tctgtgaatc cttctgggat ggcgatcctt gtatctttat catagaaatt tgttaataca
     3181 agcagctgct gtccttcaaa ttggcgaaga aagccataaa cttccggatg atttgcttca
     3241 taagcatgat aatctccttg tgcaacgatt ggatatgttt ttctcagccg aatcaatttc
     3301 ttgtaatagg acaaaataga atcttcagaa gatagttctt tttctacatt aatctcttct
     3361 agcttgcctc caacttttaa ccaaggtttc cctgtagtaa atcctgcttg ttctgtgtct
     3421 gaccattgca tgggtgttcg agagttatcg cgagattttg ctttgatccg acgaaacgct
     3481 tctttttctg tgaatccttg ttcaaggagt aacttataag cattcaaact ttctacgtca
     3541 atatagtcgg aaatcgatga atagtctggg tcgatcatgc cgatttcttc tcccatataa
     3601 atatagggcg tcccacggtt caaatggatc atggttgcta gcatctctgc tccttgtttt
     3661 cgatactgat cgataactac aaatcgattg agcgcacgtg gctgatcatg attattaaaa
     3721 aatagagcat tccagccgtt accctcactc atattttctc cccatgtatg aaatagtcgc
     3781 tttaattctg caaaatcaaa tggaggagta gaccatttat tcccattttc ataatcgact
     3841 ttcaaatgat ggaaattaaa gaccattgat aattcttctc tactaggttc tgtatacaag
     3901 atacaatttt cgatcgtcgt agagctcatt tcaccgactg tgatgatagt ggaatccgtc
     3961 ccaaacgttg cttggttcaa ttcttttaga tacgtatggg tgattggttt gtccgtatat
     4021 tcaggtttgc catcattttc tttattattt ttcaattgtt cgtctttacc gataacattg
     4081 ataacatcga aacggaatcc ttttacccct ttttccatcc aaaaacgaac aactttgaat
     4141 agttcctctc ttactgcttg gttccgccag ttcaagtcag cttgtgtccg atcatataag
     4201 tgcaaataat atttccccgt atctccaaat ggcgaccaag cacttccacc aaattttgat
     4261 acccaatccg ttggctcttc tcttaaaatg aagaagtcct gataatgctg gtcccctgct
     4321 agtgcttttt tgaaccattc atgttctgtc gaaacgtgat tcagcaccat atcgagcatg
     4381 atttctatcc ggtatttctt tgcttcggct attagctgtt cgaattcctc catcgtacca
     4441 aagataggat cgatggctgt gtaatcagag atatcataac cgttgtcttt ttgagggctt
     4501 ggataaatag ggtttagcca aatcatgtcg atgcctagat cattcagata cgggagtttt
     4561 tctttgattc cttttaaatc tccaattcca tcaccattac tgtctttgta agatttggga
     4621 tagatttggt aaatgacttt gtctttaaat gtcatggata tttacctcca ttcaactgat
     4681 ttattaccta ccacagctta tatatacaag ttatcattta ttattataac tgaacagaaa
     4741 gcgtttgtaa aatttatctt tatacattct aagaggtctg ttgcccaata aaaaacaaga
     4801 gatcagacat gtatttgtct atcctcttgt ttaaaatagc aaaatattct tgtttcttct
     4861 ataaatggtg ttcaaaagct tttatctacg gcaatcagtc ttctcaaatg taaaacatga
     4921 ggagctaatt ttatttgagt attctttttg ctcgatgcag gacggctttt ttacaacctc
     4981 tgattcatgg tgttcaatta gcagctgacc ctcgttatta gctcaaaacg ctgatttcac
     5041 aacctctgtt tttattcatt cgatttcaac gcatcaatca tatcgatatg ctttaatttg
     5101 aaatgggtaa caatcatcac aataaccgta aatacgatag ttaatcctgc tgataagaca
     5161 taagcccacc atgtgatcac taatggaaag atgacattct ccatagacgc ttgttgcaag
     5221 atgaagtcag tcaatatata tcctacacca aatcctccga tgattcctaa aagagtaaag
     5281 atgatatttt ctcgaacaat gtacatcgtg acttctttat caaaaaatcc aaggactttt
     5341 attgtagata attcgcgtac acgctcggaa atattgatat tagttagatt atacaagacg
     5401 ataaaagcca gcagtcctga taatacgaca aagattaaga caaccgagtc taagttcgcc
     5461 attgattctt cttgcgtttc gatttgtttg gacatgaaag acgtatttgt cacagcgtct
     5521 gtggcaagca gtttctcgga aagcatttct tcttcttttg ttgtcatctt atctgtacgc
     5581 agcaaaaatg cattatccat catcgattga tcagtaactg aagcataata ttcaggggac
     5641 atataaataa agctacctag atagtttttc acgatttctt ttacttcgac tttccattct
     5701 ttttgatctt catcataaag cgaaatatga tctcctgtag ataagtcaaa tagttctgct
     5761 actttttgcg taatagcgat tcctgtttta ggcaaggagc gtgggttctc ttcaagatca
     5821 gttaaatgga taaaacgatc caaatcagct gttttacttg gtacgatcac tgtcagactt
     5881 tgcgaagcat gtcctttttt tctcaattct acggtttgag tatgaatagc taggatctca
     5941 tttacttttt cttgcttctg cagcatatcc gctgcttttt gacgactatc ttccgtggat
     6001 gaatccaatg taacgattgc ttgatagtca atgatcggtc caaattgttt ggctgaaaca
     6061 gaacttagag aatctttcaa tcccacgcct gcaaccatca gcccggcaca tccagcaatt
     6121 ccgataatcg ccataagcat tcttgactta tatcggaaga gatttcggta actgattttt
     6181 tgattaaagc taaggcgtga ccacagcgga gtgatgtatt caagaaaaat gcgtttccct
     6241 ggcttcggtg ctttcggttg aagcaatgca gcaggccgtt ctcttaaatc tttgaataaa
     6301 acgatcattg cagcgccaaa tgcggcaatg aagaatgcgg cagctgcttg taaaatcggc
     6361 ggccagtcat aaaaaataag cgcactccca atatcatacc gctcgttaga cagctcaaaa
     6421 atgatccttg gcaaaagatt cgttcctaat atcgttccta aaacaatacc tatccctgag
     6481 gctagacttg catagattgc atattttttt gcgatttcaa ctttcgtata acctaacgcc
     6541 tttaatgtcc cgatttccct gcggttttct tctaccatac gtgtcattgt cgtaaaggtg
     6601 atcaatgcgg caataaagaa aaagaaaaca ggaaaaacat tggcaatcgc agcgatacga
     6661 tcagacagat caccaaattc ttgaaaaccg acattttctg aacgttcatt gtataggtat
     6721 gatggtttag ttaaattttc aattttttct gcatttcgat tcaattcttc ctctgcagag
     6781 gccagttcac gttctttctc aactaattgc ctttcttgtt cagcaagttg agcttcttgt
     6841 tgaggtaatg tttgaacagc gattgttcgt tgcgcttcag gaagctgctg gatagttgct
     6901 gactgttgcg ctaactgttt tttagcatct tctaaccgtg ttcttgcttg cgttgtttgt
     6961 gctttgccat ccgctatttt ttgctgtgcc ggttctaatt cttcgttggc cgattttttc
     7021 agctcttcta agcgttcttc gggtcgatct tgtaaatact tttctgcctt ttcttgattt
     7081 tgctccattt tatctttgta agctttgctg tacgtattca aattctttac gtttgtaaaa
     7141 tcaagatata agacagaata aatatctgtt ttaaaaacgg attcaggtaa atagacaaaa
     7201 taatcgacgc taccatttcc gacgttcgcg tatcctctct ccaaattgtt gatgaacaat
     7261 ggagaacgga caaagcccgt gatggtatac tctttttctt tcaattcatc tgaatcaatc
     7321 tgataaatat caccgatttt atacccttca tcttccgctt tttcatccaa tacaagctgg
     7381 tttggttttt ccgggagttt tccatcagtt aattgtagct tgttctgctt gttatttttg
     7441 ttataagaaa atatttgaat cacttcgttt ttgccagtat tggaataata gatctgtttt
     7501 cctgtttcta cttttgcatc tgggatcttt tctgcttgag caatatcttt ttgtgtcaag
     7561 ccaccagtag aaacgatttg caaatcagaa agtcctgctg taccaacata gtgatccatc
     7621 gttttttgca tgacaggtcc agcagttttg acacctacaa ataaaagtgt tcctagcatg
     7681 acaataagga tgattgcaat gaatcgtcct ttagattgcc gaatttcgcg aaaacctgct
     7741 ttaagatatg ttcttgtttt cataaaatcc ccctaccata caatctcatc aatggatgaa
     7801 ggatgatcat ttaattcaac agaccttact ttagcatcat taatgtgaat gactctatca
     7861 gcgatgggcg ctaaagcaga attatgtgta atgatcaaaa cggtattgcc gtgttgtctg
     7921 ctagcgtttt gcaataattt caatacttgc ttgcccgttt caaaatccag cgcgcctgtc
     7981 ggttcatcac ataaaagaag tttgggattt ttagctagtg cacgagcaat tgaaaccctc
     8041 tgctgttcgc ctcctgataa ttgggacgga aagttattca atcgatgagc taggcccact
     8101 tgcctcaata cctctacagg atcaagcgca tcaggagaaa cttccgttgc caattccaca
     8161 ttttcttttg ctgttagatt aggaacgaga ttgtagaatt ggaaaacgaa ccctacatct
     8221 gttcgtcggt aagcagtaag ctgtttatct gaaaattgcg caatatctgt gtcatcaata
     8281 atgatctgac cttcatcagg tgtatccatc ccacctaaaa tattaagaat cgttgacttt
     8341 cctgctccac ttggaccaag tataatcact aattcgcctt tttcaattga aaaagaaata
     8401 tcgtcatttg ctgtaatggt cgtttctccc attttgtatc gtttgtattc attttttact
     8461 tcgacatact tcactctgat tcctccattt tcacgatatt tctcgctata tctatgcatc
     8521 gaatgcgttt tctcacactt atttatttta ccactgaatt tctctagact aaagaaagga
     8581 atcctttaat ttagttgaca aataaacgat ttatgattta cttacataaa aaacattgtt
     8641 atttgaaaga aggaacatca tgaatgattt aaaggaaaat atgatttttg atttatcaaa
     8701 aactgctttt gtcgcgatcg acatgcaaaa agagatcgta aataacggta cactctctcc
     8761 acacactgca gcatctattc tgacaaacaa tgatttgtta gtaagaacat tgaaacgtac
     8821 cgctgccttg aaagttttag tgaacgtaga catctccact ttcccttatt tgagtcaaca
     8881 aactgatatg ggtggaatga ctgcttctgt acctccagaa ttcactgatt tacttttgaa
     8941 ggattctttg aaagatacag ataatatgtt gactatcaaa aaatacaatc caagtgcttt
     9001 ttttggtact tctcttgatc ttcaacttcg ccggcgtgga atcgaaacta tcattttaag
     9061 cggtgtagcg acaacaaacg gtgtatatgc tactgcatta gatgcctttc aacacgggta
     9121 ccatatcgtg cttgctgaag atgcttgcag tgatcgtgac aaagaatccc atcaattgtt
     9181 cataaaaaag atatttccga aaactgcacg agtacgctca acgaaacaaa taatcgaagc
     9241 tatccaacaa agcaaataag tactggcaca cgtgattttc atttacttct ttttgatttc
     9301 atgggaaaat aaccacagga tttagatagg agtatgaaca tggtgaaaaa aaagatcaca
     9361 atcaaagaag tcgctaaact ttccggcgtt tcaatcacaa ctgtctcctt gatattaaat
     9421 ggaaatacgg agaaattcag tccgaaaact gttcagaaag ttttagctgc caaagaagaa
     9481 ttaggctatc agcctgacta ttttgctaga caaatgatta ccaaggaaac gaaaacaatt
     9541 ggtgtactcg ttcctgatat cacgaatcca ttcttcagta cactgatgcg aggaattgag
     9601 gatatcttgt ataagcagaa tttcgtcacg atattatgta atgctgattc tgatcatcaa
     9661 aaagaaatcg aatatctagc cgagctcaca cgtcgaggtg tggatggatt cattatcgcg
     9721 acttctgctg tctctacaga tgcaatcaac gaaaatttga aaaaacaagg ccggcccttc
     9781 attgttttgg atcagaaaaa atctgaagga tttagtgatg ccgtacgaac agacgatttt
     9841 cgcggtggtt acctagcggg catgcacttg cttagtctag gacatcaaac gatcgccctt
     9901 gtctatcctg aaaatccacc ggaaaatgtc cacgctcgga ttgaggggtt taaatctgct
     9961 cttgatgttt accaaatccc tcacgaccaa ctgatcctct tacctacaca gttctcaaaa
    10021 caaggtggct accagatcac agcagaactt cttgattctg ctgcaactgg tgtgtttgct
    10081 ttaaatgatg aattagcttt tggattatat cgcggattag aggaagctgg aaaaagcatc
    10141 cctgaagact acagcattat tggctatgat aatatagata tgtgcgagta tatcaagccg
    10201 aaactgacaa cgatcgcaca accaatcttc gaattgggac aaacatctgc caagttattg
    10261 ttagatcgga tccaattccc agaaaaagaa tgggaagaaa aaagattacc tgtacgatta
    10321 gaaaaaagat tttcaactgc acccttgaaa tagctttcaa ggtgtgctat atttttatcg
    10381 aactattaaa acgttttact aaaacgtttt aaaaaataat tttcccatca aaataaatga
    10441 gatagttgga ggtttgtaaa atgaacacgg ttacagtaat tggaagtatt aatatggaca
    10501 ctacccttcg tttaaccaac atgcccaagc caggcgaaac gatgcatgcg catgagattt
    10561 ttcacgctgg cggaggtaaa ggtgccaacc aagcagtggc agccaaacgt tcaggcgcac
    10621 ggacatcttt tattggaggc gttggggctg acagcgaagg acaacagtta ttagatctac
    10681 tgacaaaaga aaacatcgat acatctggga tagctgaaat ccaaggcgcc acaaccggac
    10741 aagcaatgat tatggtagac gcagcaggag aaaatagtat cttgatccat gcaggggcaa
    10801 ataatgcttt ccatgaacaa gaagtcctaa aaaacaagca actgattaca aacagtgact
    10861 ttattattgc tcagtttgaa agtagcttag atgcgacgat cctagctttt tctattgcaa
    10921 aagacgcagg aaaaacaacg atcctgaatc ctgcaccagc tcgtgaaacg attcctactg
    10981 aactacttga aaagacagat atcattatac caaatgaaac agaaacagaa atcattacag
    11041 gaatcagagt gacagatcat aacagtttgg tcgcagcagc cgaaaaactc catgaattag
    11101 gtattggaac agtaatcatt acactaggca gtgccggcgc tttctatcat acagaaaaag
    11161 aacacggtat cgtccctgct tttaaagtgg atgccgttga cacaacagcc gcaggtgata
    11221 catttattgg cgcattaagc agcacacttc agccagacct tagtaacttg aaagaagcga
    11281 tcctttatgg aaatctagcc tcttctgttg ctgtgcagtc ttatggtgcg caaccttcta
    11341 tcccttaccg cgaagcttta gaacaatcag cagtctaaaa ttaacaatga gaggaagaaa
    11401 caaacatgaa aaaaggaaaa gtaatcaatt cagatatttc acgagtaatc gcacaaatgg
    11461 ggcactttga taaattaagt atcggggatg caggcatgcc tgtccccttt ggtactgaaa
    11521 aaatagattt ggctgtctca aacggtcttc caagtttttt atctgtttta gaaaacgttt
    11581 tagaagaact tgaggtacaa tcgattgaat tggcggaaga aataaagaca atgaacccgg
    11641 aaatattgga aaaaatcaaa ttattgttac cagatacacc gattagcttt atcccacatg
    11701 aagaaatgaa aaaagagttg aataactgtc atgcatttat tcgtacaggc gaaatgacac
    11761 cttacgccaa tattatatta gtgagtggag tggtcttcta atggaaacga caatgaatcc
    11821 tgaacagatg gtactgtccg agcagattca tatgctgatt gaacagttgg gaggaaaaga
    11881 caatattgat tctgtccttc catgtgcgac tcggcttcgt atcaaattga aaaaacagcc
    11941 agtagccaat gcagaaaaat taaaacagca aatcccttcc attaagtatt tcattttact
    12001 cgatagatat gcagaaataa tcatggatca gggattggct cctgcttaca aggaattgac
    12061 gcgtcaaatc gatcattcag ctccgaaaga aaagctaaat ccgatcgaaa aactatttgg
    12121 tttgatatcg gaaatcttta cgccgatcat tccgctgttt gccggttctg gtatattaaa
    12181 aggtctggtt gttttagcga ctacgattgg ctggcttagt gaaacaagcg gaacttatca
    12241 tattttgtct gctgcgtcga atgccgtctt ttattttctg ccaatctttc ttgctttttc
    12301 tgctgccaaa acgtttaaag tgaatggata tattgcggct gtcattgctg cagcactgat
    12361 tgaaccgaat ctgacctctt tactttcaga cagtggcaaa gcagctactg attttctggg
    12421 gattcctgta gtactgatgc agtatacgtc tactgtcatg ccggcgattt taggtatttt
    12481 cttttattct tttgttgaaa aattcctcaa aaaatatatt cgcgaaaata tgcaattagt
    12541 atttgtacct ttactttcat tagttatcac ggttcctttg acgttaatgg tattcgggcc
    12601 tgcgggtgtt taccttggcg aaggtttagc tgccgcaatt acttggatga tggatattaa
    12661 cggaccgatc agtggcgcaa tcatcgctgg tggttggaat attttggtaa tatttggtat
    12721 ccaatgggct gtaaaccctg tcatgatcag taatatctct gcttatgggt tcgaccgaat
    12781 cgttccgttg acaggagcag ccaattttgg aatggctggt gcagctttag gtgtatttct
    12841 gcgaagtaaa cgttcaaaaa cccgttcgat ctccggttct gcttttgctt cgattttact
    12901 tgcgggagtg accgaaccaa ctgtttacgg aattgcgatc ccattgaaaa aaccatttgt
    12961 tgctgcttgc attggtgcag ctgctggtgg agctgtaatg ggatttgccc aagtgaaagc
    13021 gatcgctttt gtatttggta gtctgaccac attgcctgca ttcatttccg gtactttttt
    13081 ctggtactta gcaggactgg ctgttagtct agtcgtagca atgatcacta ctctcgttag
    13141 cgggtttgat gaagacctaa tgtcttatga atagttaatg aaaaagactg aagtatgaga
    13201 tgtttatcac atatttcagt ctttttcttt ttaggatcat tatttttata aatttttgtg
    13261 atactttcca aactttggta
//
LOCUS       DACDKL010000067        13268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDKL010000067 DACDKL010000000
VERSION     DACDKL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.067"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            258..1451
                     /locus_tag="IV329_001464"
     CDS             258..1451
                     /locus_tag="IV329_001464"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8940646.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1581..3053
                     /locus_tag="IV329_001465"
     CDS             1581..3053
                     /locus_tag="IV329_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8940647.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3136..3924
                     /locus_tag="IV329_001466"
     CDS             3136..3924
                     /locus_tag="IV329_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8940648.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3991..4659)
                     /locus_tag="IV329_001467"
     CDS             complement(3991..4659)
                     /locus_tag="IV329_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8940649.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4774..6486
                     /locus_tag="IV329_001468"
     CDS             4774..6486
                     /locus_tag="IV329_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940650.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6489..8258
                     /locus_tag="IV329_001469"
     CDS             6489..8258
                     /locus_tag="IV329_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940651.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8463..9029)
                     /locus_tag="IV329_001470"
     CDS             complement(8463..9029)
                     /locus_tag="IV329_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8940652.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9029..9985)
                     /locus_tag="IV329_001471"
     CDS             complement(9029..9985)
                     /locus_tag="IV329_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8940653.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9985..10641)
                     /locus_tag="IV329_001472"
     CDS             complement(9985..10641)
                     /locus_tag="IV329_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8940654.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11011..13029
                     /locus_tag="IV329_001473"
     CDS             11011..13029
                     /locus_tag="IV329_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8940655.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT         4161 a         2382 c         2794 g         3931 t
ORIGIN      
        1 actttttgag tatctttttc tttttatctg aaagaatgat tttaaaaagc ttagcagagt
       61 tatctttatg gatgattgtg ctaagctttt ttggacagtt ctaatgtaaa atatttttat
      121 attttttaag caaaaaaatg aaaaaatacg agcagaaaac tgttttatct gctatacttg
      181 gttcgttgca agttcccgaa aggatccgca ggacggagat gccttgtaac cgaatttaac
      241 tagggggaat cattataatg gtagaaagac acttatttac atcagaatct gtttctgaag
      301 gacatccaga taaaattgct gaccaaatca gtgatgcaat cttggatgca attttaaaac
      361 aagatccaac agcacgagta gcgtgtgaaa cgtctgtaac aactggtctt gttttagtat
      421 ttggagaaat atctacgacg gcatatgtag atattcaaaa agtcgtacgg gaaacaataa
      481 aagaaattgg ttatacacgt gcaaaattcg gatttgatgg agatacagca gctgtattgg
      541 ttgcgattga tgaacaatct cctgatattg ctcaaggagt tgatgaagca cttgagatcc
      601 gggatgaaga taaaaaagac gtattagatg agattggtgc tggtgaccaa ggtttaatgt
      661 ttgggtttgc tgtagatgaa acaccagaat tgatgccttt accaatcgct ttgagtcatc
      721 gtctggtacg acgcttggca gatttgcgta aatcaaatga attaacgtat ttacgtccag
      781 atgcaaaatc tcaagtaacg gttgaatatg atgatcaagg acaaccggaa cgcgtagata
      841 caatcgttat ttcaacacag catgatgatg cagtagataa tgaaacaatt cgtcatgatg
      901 tcatcgaaaa agtagtgaag gaagttattc cagctgaatt attagatgat caaacgaaat
      961 attatatcaa tccgactggc cgatttgtca ttggtggtcc tcaaggggat gccggattaa
     1021 caggaagaaa aatcattgtt gatacgtatg gcggttatgc tcgtcatggt ggcggtgctt
     1081 tttctggtaa agatgcgaca aaagttgacc gttctgccag ctatgctgca cgttatattg
     1141 ctaaaaacat tgttgcagca gggcttgctc gcaaagcaga agtacagcta gcttatgcga
     1201 ttggtgttgc tcaacctgtt tcgatctcaa tcaatacttt cggtactgga acggttccgg
     1261 aagaagaact gattgctgca gtgagagaaa acttcgatct tcggccagca ggaatcatcg
     1321 agatgcttga tttgcgtcgt ccgatttaca aacaaacagc tgcttacggt cattttggtc
     1381 gtacagatgt agatttgcct tgggaacata cggacaaagt agatgcgttg aaagcaagtt
     1441 tagcaaaata agttatattc agctggaacg atcgccaagt ggtggttgtt ccagcttttt
     1501 ttatggcagt taacctcttg aaaaagatag taattattta tctattttta gatcctgtca
     1561 taatgaaagg gaggaataag atgaaaaaag aaacaaatgt tactttggtc acagtgagta
     1621 ttttcattgc aacgttcatg acggctattg aaggtacaat cgtaacaacc gctatgccaa
     1681 cgattgtcgg ttcattacat ggaatggaaa tcatgaactg ggtattttcg atttatttgc
     1741 tgacaaacgc tatgcttact ccgatttacg gaaaacttgc ggataaagtg ggacggaaac
     1801 caatctttat ttttgggaca ttattgttta ttatcggttc gttattttgc ggtttatctc
     1861 aaagcatgat tgggttgatt gctgctcgtg cgattcaagg gatgggtgct ggggcaatga
     1921 tgccagtagc tttgacgatc atcggcgatc tttattctat tgaaaagcga gcaaaagttt
     1981 taggtttgaa tagttcagcc tgggggattg ccagtgtttt tggaccgctt gcgggaggat
     2041 tcatcgttga aacgatcagc tggcactgga tcttttttat caatgtgccg atcggaattc
     2101 tattaattat tttgatttgg ctatatttaa atgaaccaaa aatcatacat gagtctaaac
     2161 caatggatat ttttggtagt tttagtttga tggccatgct tgtctcattg ctgttagggt
     2221 tccaattatt aggagacgaa ggaatgagtc cgcttgtttc tgctttgctg ctaggtagta
     2281 tcgtaatgtt aggtattttt gtttttgccg aaaaaagagc taaagatcct gtcatcatga
     2341 tgcatttatt caaaaatcca ctatttgttg tggtcaactt ggctgcagca ttggctagtg
     2401 gttttttgat gggaatcgat gtttatatcc ctatgtggat gcaaggtgta ttagggctga
     2461 atgcagcaat tggtgggctg gtattagcac cattatcact agtctggatg gtcggttctt
     2521 tccttgcaag caagtggatg actcaaatga aaactcattg ggtcttgaga attggactac
     2581 taatcgcagc tattgggggc gcttggttgt atcttatgcc tttcagaagt aactatttat
     2641 ggttttttgc tatatcaacg gtcatcggta taggcttagg tgtgaccatg actacctcga
     2701 ctgtcagtgc acaaaatagt gtatctcgtg accagcttgg tgtagcaact tcttttaata
     2761 cgttagttcg aacaattggt caaacagtca tggtttctat ttttggttta ttgataaatg
     2821 gggtaactaa acgagaactt gcagcagcaa atttaacaaa tgatgcagat atcatgaata
     2881 aactggtgaa tccacaaacg gctaaatata ttgatgcgga tattttgtta ccattaagaa
     2941 aaattcttta tgatgggtta cattccgttt atctagttgg cttgttactc gtgatcattg
     3001 ctttggtctt aacgcttttc gttcatgaaa aaagtggtaa aataaaacag tagtttcggt
     3061 taagatatgt acgtagccga atcattcaaa tttaaagtta ttcaaaaata acttcttact
     3121 aaggaaggcg aaaatatggc atttattcaa gcaaatattt attcgaatgt gttagaaatg
     3181 gaagtcaatc taaatgtgat actaccgcaa acaacaaaaa aggtgattgg caccagttca
     3241 gagcaagaga caacggatat acctgtttta tatttacttc atggaatggg tggcaatcat
     3301 agtgtatggg aaagacgtac gtcgattgaa cgttatgtag caaactacgg attagctgtc
     3361 atcatgccat ctactgatct tggctggtac acagatacag tttatgacat gaagtattgg
     3421 acattcattg cagaagagct acctgagatt tgtcatgaat tattcccgca attaacaaga
     3481 aaaagagaaa aaacatatgc cgctggatta tcgatgggcg gatatggcgc gttaaaacta
     3541 ggccttgcaa aatcagaaaa tttcgcagca gtaatctctc tttcaggcgc tgtttctgta
     3601 ggaaatcgaa tggatgatct tttgatggtt cgtaaagcat ctttttggga agggatcttt
     3661 ggaccattgg atcaaatcga aggttcaaaa aatgatccgc aatacttatt gaaagaacta
     3721 gtaaaaagcg gaaaaccagt tccaagattt tatatggctt gtggggaatc tgattttctt
     3781 tatcaggcaa atcaggaaat ggtatctgct atgcaggaac aaggcttggc ggtcaccttt
     3841 gaacaaggac ctggcgatca taactggatc ttctgggacc aatggattca acgtgcattg
     3901 gcgtggctaa tggaagaaaa ataaaaaaag agtcttaaaa agatagctcc tatagagatc
     3961 acaaacatag tgatctctat aggagctttt ttactgctcg ttttctcttc ttttattttg
     4021 ttggcataag ccttcaatat aggtttgtgc actcgttaga aagagtgctt tttcattcaa
     4081 ttctgcatgg gactgcatac tttctttaaa tccctctaat tgattttcaa ctacataatt
     4141 acgcataaat agaactgttt ctttcaaaaa ttcatttccg tccataattt tttgtcgaag
     4201 ctgctgttca tgctgctgat ccattaactg tccaatcaat aaattgtgga gagaggcaat
     4261 ggcctgatga ctttgggtga ctgaaaatcc ttcacgaaca agtaattgga tcatttgctc
     4321 caaatgttct aatcgactcg gaaaagcggg cacagtattg atttctaatt ctgccccaca
     4381 agggaaacgt gtataaaggt catagtaatt ttccatatac tgcagcaact gttcattcca
     4441 aggcaaagtt ggatctggaa gcgctaattc ttcttccatt gtttcagcca ttatttgcaa
     4501 gagctgttgt ttgtttttga aataccaata taaagcaggt gcttgaacat gaattctgtt
     4561 tgcgatgttt cgcatggaaa gctgcgaaag agcttgtgtt tcagccagga tatcaaaggc
     4621 ggcctgaatg atgattgctt gagaaagctt tatttccatt ttatcaacct cgtgtattgc
     4681 attatctttt taaacatggt aaattgttag tcggtctaat tttacagtgt taaattagtc
     4741 ctgtcaattt aaacaaaggg gtgtaatcaa cgaatgaaat taatcttacg acatgcgaaa
     4801 aaatataaag tagctgtttt tatttccttg ctgtccgtag cagtcatggt tgcagcagca
     4861 ttatggcaac ctaagttact acagcaagta ttggaagcta ttattacaga agataatgat
     4921 gaaatgaaaa caattggtat ttacctgatc agtctggctt tgctaggatt agccgcaggt
     4981 gtaacgaaca cgattttctc tgcaaaagta gctcaaggcg taagtgcaga tatccgagaa
     5041 gaagcatttc gaaagatcca gacattttca tttggaaata tcgaacagtt ctctgctgga
     5101 aatcttgttg ttcgtctaac aaatgatatc acgcaaatcc aaaatttagt gatgatttct
     5161 ttgcaatcac tttttcgaat tccattctta tttattggtg ccttcatttt agctatgatc
     5221 acgatgccgc aattatggtg gatcatcgta gcgcttatcg ttacggtatt tctgattacg
     5281 gcactttcat ttacaaggat gggcaagcac ttcatgatca ttcaaaaact gattgatcgt
     5341 gtgaatagca ttgcaaaaga aaacttacta gggattcgtg tagtgaaatc ttttgtacaa
     5401 gaagaaaacc aattgaagtc attcagcaaa gtaagtgagg acttaacaaa acacaacatc
     5461 atagtcggta cattattttc tgtgatgatc ccgtccttca tgctggctgc caatttggct
     5521 gtggttgggg cgatcttttt tgtgagcgat cttgttaaag atgatcctac gttaattggt
     5581 ggaattgctt cattcatgaa ctacttgatg cagattatga tggcaatcat tattggcgga
     5641 atgatgatga tgatgacttc aagagcagcc gtatcgctga agcggattgc ggaaatttta
     5701 gatgctgaac cagatcttac ttatttagac gttccagaac aagagttgac gggttctgtc
     5761 acttttgacc atgtttcttt tcgttatcct ggtgaggata cagatacgct taaagatatc
     5821 agcttttcta tcaaacctgg agaaatggtt ggtatcgtcg gggcgactgg tgccgggaag
     5881 tcaacactag cacaattgat ccctcgtcta tttgatccga cagaaggcaa agttgaagta
     5941 ggtggtgtag atcttcgtca agtaaatgaa aagagtttaa gacaaacggt ttcctttgtt
     6001 ttgcaaaaag caattttatt ctcaggaacg attgctcaaa acttgcgcca agggaaaaag
     6061 aatgcaacag aaaaagagat ggaacatgct tcttctattg ctcaagcaaa agaattcatc
     6121 gaaaagttat ctgatcgtta cgaagcacca atagaagaac gtagcagtaa cttttctggt
     6181 ggtcaaaagc aacgtttatc cattactcgt ggcgttatcg gtaatccaaa gatcttgatc
     6241 ctagatgaca gtacgagtgc actggatgct cgctctgaaa agctggtccg agaagcactc
     6301 gatcgagatt tgaagggaac aacgacgatt gtcatcgcac aaaaaattgc ttctgttgtc
     6361 aaagcagacc gcatcttagt attggatgaa ggcagattag tcggcgaagg aacccatgag
     6421 gaattggtag ctggaaaccg tgtctatcag gaaatctttg aaactcaaaa aggaacggag
     6481 gagtaagtat gactgatcta ataaaagcaa gtaaattttt ctatcactat ttgaagcggt
     6541 acaaactctc ctttttcttt atctttatca cagtgatcat tgctacctat ctacaagtaa
     6601 aggcacctca atacatcggg gaagcttttc aagaattagc taattacgta ggaggcttga
     6661 tgcaaggcgt agacgataaa agcaaatttg tttctgtaat atggaaactc ttgctgtttt
     6721 atgtactagc tagtgcagcc aactttatct atagtatttt gttcacacaa gtagtaggga
     6781 agtcaacgaa ccgaatgcgt atcggtctat tcaacaaatt ggaaaaattg acgatccgtt
     6841 tctttgattc tcatcaagac ggtgaaattt taagtcgttt taccagtgac ttagataata
     6901 ttcaaaatag tttgaaccaa gctttgcttc aagtcatcac gaatgctgtt ttacttgtcg
     6961 ggatcttgat catgatgttc cgccaaaatg tagagttagc ttgggctaca attgcttcca
     7021 ctccagtagc cgttttgata gctgtcttga ttatccgcaa agcacggaaa tatgtggatc
     7081 ttcagcaaga tgaagtgggg aaactaaatg gttatatgga tgaaaaaatc agcggacaac
     7141 gtgtcatcat cacaaatggc ttacaagaag aaacgattga aggattctta gagcataatc
     7201 aaagtgttcg cgaagcaacc ttcaaaggac aagcttattc tggtctacta tttccgatga
     7261 tgcaaggaat gtcactggtc aatacagcga tagttatctt tttcggcggc tggttagcat
     7321 taaatggtga tttggaacgt tcggtggcat taggcttagt tgtgacattc gtccagtatt
     7381 cacaacaata ttaccaacca ctgatgcaga tttcatctgg atacagcatg atccaattag
     7441 cgattacagg ggcaagacga ttgaatgaaa tatttgatga aaaagatgaa gtcaaaccag
     7501 aaaactgtaa agcatttgat ggtgttgaaa aaggtcttgc gttgcagcac gtagattttg
     7561 gttattctcc agaaaaacta gtcttgaaag atgtctcgat caatgtggac aaaggagaaa
     7621 tggttgctct tgttggtcca acaggatcgg ggaaaacgac gatcatgaat ctgctaaacc
     7681 gtttttacga tgtcaatgat ggttcagtag tcatcgatgg aatagatatc cgagattatg
     7741 atcttgatgc attacgtaga catgtaggaa tcgttttgca agaatctgta ttgttctccg
     7801 gaacgatcaa agacaatatc acattcggca aaccggacgc ttcagatgaa gaagtcatca
     7861 atgcagcaaa acaagcgaat attcatgact tcatcatgac gttagaaaaa ggttatgata
     7921 cagagatatc cgaagaaaat aacttattca gtacaggtca aaagcagttg atcagtattg
     7981 ctcgaacgat catcacaaat ccatctttat tgattttgga tgaagcaaca agtaatgtag
     8041 acacggtaac agaagcaagg atccaaaaag caatggatga agcaatcaaa ggaagaacca
     8101 gctttgtgat tgctcaccgg ctgaaaacaa tcttgaatgc agatagaatc gttgtcttgc
     8161 gtgatgggga agtcattgaa gaaggcagtc atcacgagtt attagcagaa aatggatttt
     8221 atgcggaact ttatcataat caatttgtat ttgaataaag aggttgtgaa aaagctgtcc
     8281 tgcatcaagt aataagaaca gccaatcaaa aacagcttct catgtttttg atttggttgg
     8341 acttattacc gcagatataa gcttttgagc accgtgaata aagaggttat gaaatcaacg
     8401 ctctaacctg agtattagag aaaaaaacgc aaaacatatc atttatgttt tgcgtttttt
     8461 atttatcttt ttttctcaat acaatataaa ataggcggct ggttcttttg gttgataaac
     8521 cgataattca gcacattata tgtttcctgt ggcagtttgg aacaaaaaga atcaaccata
     8581 tccagttctt tttctccacc ttcatgacca tagtagacga caaggatcaa tcgtccgcga
     8641 ggcacaagtc gtttcaatat ttcttccata gctgtgcgtg tagtctctgg catagtgatg
     8701 attgctttat cactttttgg gagataccct agattgaaaa tccctgcttt gagattttgt
     8761 tgttcagcta tcacttctcc taacgtttcg tggccttgta gaaacaatga gacacgttct
     8821 tctaactgac gctgttctag cctttcacga gtagcatcga ttgcttgttg ctggatatca
     8881 aaactataga catgaccagt ttttccgatg tgttctgcta gaaaaaccgt atcgtggcca
     8941 ttgcccatcg ttgcatctac gacgtgatcc cctggttgca agatttcttg cagtaatgta
     9001 tggctgaaat gcaaagcagt ttgcaacatc agacgctcac ctttttccgt acatcatatt
     9061 ttccttggaa gctatctctg cgaagaagtt cttcatcaat tgcattcaag acttcccatt
     9121 ttttcaagct ccacattggt ccaatcaagg aatcccacgg cgcatcccct gtcaaacgat
     9181 ggatgatgat ctcttttggt atcatttcca gctggtcgca aatgacattg acatagtcag
     9241 gacggctcat taattgaagg cgaccttcat gatagtcccg caacattctc gttttgcgca
     9301 tcagatgcat caaatggagc ttgattcctt ggatatctga atctaaaatg gtgcgtctga
     9361 cattttcttt catcatttct aaactttcgc ctggtaatcc gttgatcaaa tgcgtacaaa
     9421 cacggatatt atgtttgcgt aatttagcca cgccatctaa ataagtttga taatcatgtg
     9481 ctcggttgat cagttttgaa gtttcttcaa aagttgtttg caatccaagt tctacccata
     9541 aatacatccg ctcgttcagt tcagcaaggt aatcaactac ttcatctggc agacaatccg
     9601 gtcgtgtacc gactgataat cctacgacac ctggtaaatt gactacttgc tcaaatcgtt
     9661 ctttgattat ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa acgatgtatt
     9721 gttggacatg aggccatttt tgatgcatca tgtcgatttc tttctggaat tggattggaa
     9781 gagggtcttc aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa aaagtacaac
     9841 cgccatgggc aacagtccca tctcgattcg gacagtcaaa tcctccatcg atcggcacct
     9901 tgaaaatttt cccgccaaac tcatttcgta aagcgtaatt ccaagaatga tatcttttat
     9961 ttccttctga ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt tatctatttg
    10021 ttttcttttt tctctataca ctggataagt caggcacagc caagaaagac tgagtaagta
    10081 accgccgatg atatcagtag gaaaatgaac gcccagataa atgcggctta tcccgacact
    10141 tactataata aacatcatca gcaattgaag caatgtgcgt aaggttcttt tttgaacaaa
    10201 agctggaagc agaaatagta aagaaccgta aagaatcata ctgcctgctg catgtccact
    10261 agggaaactg taacttgttt caactactaa gtgcgccaat gaaggacgct ctcgcatgaa
    10321 aaatagtttg atcaccgggt tgatgattcc agcaattaca ataaatccag aaacgaacca
    10381 aaatgtttct gccctttttt tcttatacca catccataaa gccgcacaag caaataaaat
    10441 gatgacggta agaggattac cgaatttagt cataaagagt tggtagctgt tcagatcagg
    10501 ataagaagct ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc acgacagatg
    10561 gaactttacg atccagccca aaatgaaaaa caggacaaga aagaagctac cagccacctg
    10621 aaatgatgtt ttatatttca tacaattcct cctttgaagc aatgacttat tatagcattt
    10681 ttggtccagc gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct ctttgaataa
    10741 ccaatattct tataaaaaaa ataactgtaa tcggaaagac attttttggt aatgcaaatg
    10801 aaatgttagg gaaataaaat cagtgttttt cgaaatcctt ttaaacaaga acgtagacgt
    10861 aggaagaact tgaaaatgaa cagaagttgt tcattacggt aaattacaat atgattacaa
    10921 aaaacaacaa agctcttaaa taacttttaa taaatattaa gagaagttat aattattaat
    10981 aatagataaa ttgaagttgg aggatagaag atggaaaaca ttgcaagaaa agaacggaga
    11041 cgtttagaac agaccaaacg ttttcgaaaa gcaaaacgag gggcagcaat cgtgggaact
    11101 gcgatggtag gctgttctgt cgctgcccca ttagtacagc cggttcctgt tcaagcggat
    11161 gaaacacctg ttcagttcgg cgcaagaatc aatacagcgg catttatcac agaaattgct
    11221 acttatgctc aaccaatcgc tcaagccaac gatctatatg catcagtgat gatcgcacaa
    11281 gcagttgtag aaagcggttg gggcagtagt gcgttatctc aagcacctta ttataatctt
    11341 tttgggataa aaggcagcta tcaaggacaa accgtctaca tggatacgct tgaattttta
    11401 aataatcaat gggtaaataa aaaggagcct ttccgacaat atccttcttt tgctgaatct
    11461 ttcaatgaca atgcctatgt acttagaaat acttcatttg gcaacggtta ttactatgcc
    11521 ggtacttgga agagcaacac gaaatcctat acagatgcga cagcttgttt gacagggcgt
    11581 tatgcgactg atcctggtta cgcaggcaaa ttaaataata tcatcgcaac ttacggattg
    11641 acccaatatg atacaccagc tagcggaaac gcaggcggtg gagcgactac aggtggcaat
    11701 aatactggaa gtgcaggtag tactggcggt tcaagcaatt ctggtaatac aaactcaggt
    11761 tcagcaactt cgaccactta tactgtccaa tctggtgatt ctgtttgggg aatctcaaat
    11821 aaattcggta tcacaatgga tcaattgatc caatggaaca acatccaaaa caatttcatt
    11881 tatccaggac aaaaactgac aatcaaaggt agccaggaaa atggctcttc gacgaataat
    11941 tccggtaaca ataccaattc ttcaggaaat gctggtacat caaacggagg acaaacaact
    12001 ggtgcgaaat acactgttca atctggtgat tctgtttgga agatctcaaa tgatcacgga
    12061 attacgatgg ctcaattgat tgaatggaac aatatcaaga acaatttcgt ttatccagga
    12121 caacagctaa tcgtatcaaa tggcggttct tctaatgctg gagcaaatac tggctcaaat
    12181 actgggaata caagtggctc tagttcgtcc agctcaagtt cttctaatac agcttcagga
    12241 acgaaatata cagttaaagc cggagactct gtttggagcg tctcaaataa atacggaatt
    12301 acgatggctc agctaatcca atggaacaat atccaaaata actttatcta cccaggacaa
    12361 caactgactg tttcaaacag tggatcaaca aacagcacgt caggtaatac aggttcgtca
    12421 accaatacaa ataacagcag ttcagttaat actggcagca gttcgtctca aggcacttat
    12481 accgtgaaag caggtgaatc tgtttggagc gtggcaaata aaaacggcat cacgatggat
    12541 cagttaatcg aatggaataa tatcaaaaac aacttcattt atccaggaca gcaattgatc
    12601 gttaaaggtg gaacttcggc aaatacaaat acaggttcaa ctacttctgc agcgaaacca
    12661 aatacaccga atacgtctgc tacgacaagt acatcatcca ctggtaatac gatgtatacc
    12721 gtgaaagcgg gcgagtcagt ttggagcgta gcgaacaaac atcatatcac gatggatcag
    12781 ttaatcgaat ggaataatat caaaaataat ttcatctatc caggacagca agtgattgtg
    12841 aagaaaggtt catctcaatc aacaaaccaa caatcagtta ctggttcgaa aacctatact
    12901 gtgaaatctg gagaatctgt ttggagtgtt gctgacagtc atggaattac aatggctcaa
    12961 ctaatcgaat ggaacaatat taagaacaac tttatttatc caggtcaaac gttgattgta
    13021 gctaaataaa atagctgttc gaagaaataa cgattgaatt ccacaaaaat atttgttact
    13081 ataaggctac ttaaatatga caacacttga aaaggaagta gtaaaaagac tgaagtttca
    13141 gagagcgcat ggctggtgag aatgtgcacg gatgcttttg aactcgcctt tgagttgccc
    13201 ttgctgaata cccgagtaag caagcggcgg tgacggccgt tacaacgttc gaggcctttt
    13261 cactaagg
//
LOCUS       DACDKL010000068        13141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDKL010000068 DACDKL010000000
VERSION     DACDKL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.068"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..183)
                     /locus_tag="IV329_001474"
     CDS             complement(<1..183)
                     /locus_tag="IV329_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8940656.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFF"
     gene            complement(431..1267)
                     /locus_tag="IV329_001475"
     CDS             complement(431..1267)
                     /locus_tag="IV329_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8940657.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1278..2207)
                     /locus_tag="IV329_001476"
     CDS             complement(1278..2207)
                     /locus_tag="IV329_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8940658.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2399..2752
                     /locus_tag="IV329_001477"
     CDS             2399..2752
                     /locus_tag="IV329_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8940659.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2826..3482)
                     /locus_tag="IV329_001478"
     CDS             complement(2826..3482)
                     /locus_tag="IV329_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8940660.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3708..5642
                     /locus_tag="IV329_001479"
     CDS             3708..5642
                     /locus_tag="IV329_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8940661.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5728..6951)
                     /locus_tag="IV329_001480"
     CDS             complement(5728..6951)
                     /locus_tag="IV329_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8940662.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6945..8843)
                     /locus_tag="IV329_001481"
     CDS             complement(6945..8843)
                     /locus_tag="IV329_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8940663.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8837..9955)
                     /locus_tag="IV329_001482"
     CDS             complement(8837..9955)
                     /locus_tag="IV329_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8940664.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10038..10871)
                     /locus_tag="IV329_001483"
     CDS             complement(10038..10871)
                     /locus_tag="IV329_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8940665.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10864..11781)
                     /locus_tag="IV329_001484"
     CDS             complement(10864..11781)
                     /locus_tag="IV329_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8940666.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11804..11983)
                     /locus_tag="IV329_001485"
     CDS             complement(11804..11983)
                     /locus_tag="IV329_001485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940667.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12008..13132)
                     /locus_tag="IV329_001486"
     CDS             complement(12008..13132)
                     /locus_tag="IV329_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8940668.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4119 a         2642 c         2171 g         4209 t
ORIGIN      
        1 gaaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa attgtataac
       61 gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg attcttccaa
      121 aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata tacgcgttac
      181 cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca acttttcaac
      241 atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc tttgaaaccg
      301 atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg aatacatcat
      361 gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta tgtttattta
      421 ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag actttctcag
      481 cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag tctgattgga
      541 tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc ggcatatgcg
      601 tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat cggaacaaga
      661 agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag ctgatttttt
      721 tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa tagttttcat
      781 ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc atttggtcta
      841 agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt gtctgcatct
      901 ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct tctacgatcg
      961 tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa gctagcagta
     1021 cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg tgctcgtgtg
     1081 ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa gccaaaagag
     1141 cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata tattgatcga
     1201 gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa atcaaaaacc
     1261 gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag ggcaatcagt
     1321 ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag tagatttttg
     1381 atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc gaacagactt
     1441 aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt aaaggccaat
     1501 aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat aattctagtt
     1561 ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata acgacctgta
     1621 tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat cagctgattt
     1681 tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg attttctgct
     1741 actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc aagccagaca
     1801 ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat ctgccaagaa
     1861 ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg agcagtcagc
     1921 ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg aacgatcggt
     1981 acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt aaaaatagag
     2041 aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa tacttgtaag
     2101 gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac gatcgataag
     2161 aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag tttcctcctt
     2221 tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa acaagttgct
     2281 ctgtaaattt ttacacaact cttatttttt accaaagcac ttcaattgac tttttctgtt
     2341 attcccatta aattaaggtc tcatacatat cacttctatc ataaaaggga gaataaaaat
     2401 gaaacaaacc actaacttga ctgaagttca agacatactg caacagtcat ccgttgccgt
     2461 catttatctt tcgatgccta attgcagtgt ttgtcacgct gttcgtccac gactagagga
     2521 acttctcaca cattatgata ttccggcact tcatttggat gcatttgaaa caccagaagt
     2581 agctagtaaa tttgaagtgc tgactgcgcc agttgtcctg atttttcatc aaggaaaaga
     2641 agtctttcgg caagcacggt tcatcgactt taaaaaaatc cgttatctac tagatgaact
     2701 gacagcagaa gatgattccc tttcttatga agaaatcttc cgaacaaaat aatcgttgtc
     2761 caaacagaaa aaagcgattg aacaagtgaa agttttgcct tcatttgttc aatcgctttt
     2821 tatttttatt ctgctgtatt ttcggaatgc gcatttgcgt cagaatgcaa gaaatagatc
     2881 aaggtttgca attcattcgt caaatcaaca ttttggatca atacatctga cggagcattc
     2941 aatcgagcag cggtaaagtt caagattcct ttgattcctg cttccgttaa ctgattgact
     3001 acttcttgtg ctctttcagc gggtatcgtt aaaattgcta cttcgatttg ttggatcttg
     3061 atttgttctg acatatcttc cattggataa acggggatac cgtctacgat gcgcccaacg
     3121 atttcctcat tcacatcaaa agcacaactg atacgtatgc tattactttg atggaatttg
     3181 tattttagta aagcacttcc caagttccct acaccgatca gcgcaacatt tgtcaattga
     3241 tcgtcgttta atgtctttgc aaaaaagtgc ataagatctt ctacatcata accataccca
     3301 cgttttccta attcgccaaa ataagaaaaa tctcgacgga tcgttgcact atctacttga
     3361 acggcttcac ttaattcagt tgaagacact tttttctttc cagcattatt taaaatcctt
     3421 aaatagcgat aatacaaagg cagtctttta gctgtcgcct ttggaataat atgatctttc
     3481 acgaaatgac ctccacgtat tgtctaatta catctttgat ttctccatca tagcagtatt
     3541 tataggaaaa aatcaattcc attgctcaga tattgtgaaa ttttattctt tattcattta
     3601 tttatcattt ctgcaaatct cgcagtataa tggaatttat ggtagactac taattgaaaa
     3661 atagtttttt atttgaggag aattcacaaa gtagaaaaga ggattccatg atattattac
     3721 aagccaatca gattgctcgc cattttggtg cagaagtctt atttgaaaac atacatttag
     3781 aaatcagtac aggcgctcgt gtcgctttag ttggcagaaa tggcgctgga aaatcgactt
     3841 tattaaagat cattgctgga attgaagcac ctgatgaagg aaagatcgct aaaaataaaa
     3901 cagcgactct cggatacctc gctcaggaca caggacttga ttccaatgaa acagtttgga
     3961 acgagatgct gaaagctttt gaacaagtta gacaaatgga agagcggatg cgcgagctag
     4021 agattttcat tagcgaaggt acgccagaca cacctgctta tagttcttat ttaaaagaat
     4081 acgaccggct gcaacacgat ttttcagatc gaaacggcta tggttatgaa aatgaaattc
     4141 gttccgtatt gcatgggttt aaattcgatt cttcatttta tgatcagaaa atcgatactt
     4201 tatcaggcgg acagcgcaca cgtttagctt tagctcgcat gcttttgcaa caccctgaca
     4261 ttttgattct agatgaacca acgaaccatt tagatatcga gacactttcc tggcttgaga
     4321 attatctgca aagctatact ggtgcattat tgattgtttc ccatgaccgt tattttttag
     4381 ataaagtagt caatgaagtc tatgaattaa gcagaagaaa aatgcgccat tacaaaggta
     4441 actattcgaa atatctagat ttgaaagcag aacagttagc aagtgaatgg aaggcttatg
     4501 aaaagcagca aacagaaatc cataaattag aagacttcgt tgctcggaac cttgttcgcg
     4561 cctctaccac aaaacgagcg caaagccgca gaaagacatt agaaaaaatg gaccggctgg
     4621 atcgtcctca aggggatgaa aaacaagcaa actttctatt cagtatagaa aagccttctg
     4681 gaaatgtagt cttgcaaatc gaagatgccg ctatcggata tgataatgtc accttatctg
     4741 aaccaatcaa tctagatatc cgtcgtcaag aagcgattgc tctagtaggt ccgaatggta
     4801 tcgggaaatc gacgttgctg aaatcaatca ttcaccagat cccttttatc cgtggaaaag
     4861 aaacgctagg gacaaatgtc accatcggct attacgatca aggtcaagct gatctgcata
     4921 caaacaagac gatcctagca gaattgtggg atgagcatcc taccactcca gaaaaagaga
     4981 tccgaagtgc tttaggtgct ttcttatttt ctggcgaaga tgtagaaaaa ccaatccctc
     5041 tgctaagtgg cggtgaaaaa gcacgggttg ctttagccaa gttagcgatg aacaaagaaa
     5101 attttttgat tttggatgaa ccaacaaacc atttagatat tgacaacaaa gaagtattag
     5161 aaaatgcatt gatcgattat gacggcacac tgctttttgt ttcccatgac cgatatttca
     5221 tcaatcggat cgcgacaaaa gtcgtcgaac tctctgaaaa tggcagcaaa gtctatctag
     5281 gagactatga ttattattta gagaaaaagc aggaagaaga agaaatcgcc gaattgttgg
     5341 cacaagatga gccagaatct gctcctgctc ccacgaacaa atctgattat taccaaagca
     5401 aagaacagca aaagcttatc cgtagccttc aacgaaaaat tactcaggtt gaagaagaaa
     5461 tggcgcggat cgatgaatgg atcgatcagt tggaacaaga aatggtcgcg ccagaaaacc
     5521 tggaagatca cgtaaaactc aatgagctga accaagattt agaagctgct agacaagatc
     5581 aagaaacaaa attgacggaa tgggaagaac taagcttgga attagaagaa tatgaaaatt
     5641 aacgaaaaaa ccaaaacaga aaccattcat gctgttttgg tttttctttt tttattttat
     5701 caaacaaaaa ttccatattt acctttttca ccaataagaa gaccaattac tagtcattct
     5761 cactaaagct tctaaataat agtaatcacc ccaaatacaa ggtgtattta ccccattatt
     5821 tttgggctta ttataaacag actgtgttaa aaagccatct tcttcttccg ttgtagcata
     5881 tctttctttc aattgtcgca taattttcca aacagcagac tggtataagt tcttttctgt
     5941 tttgtctaat agacctgatt ctattaattc taataatcca caaaccatga tcaccatact
     6001 agatgtatct ttttcttctc tgctagaatc atcaaagatc aaatcccaat aacaaatggt
     6061 atcttctggc aaccgattca taaaataatt ggctaatatt ttgcttaaat caaaatattt
     6121 ttgttctttc aaataacgat aacttaatgc aaaaccgtaa atcccccatg cttggcctct
     6181 agcccaacag gaattatcac tataaccttg atgcgtactt ccatatcttg gttttccatc
     6241 tttgacatcc atataaaatg tatgataagt tgaaccatct tctcttacta aataatctgc
     6301 tgcattttga atatgagcct gagcagcttt tttatatttt tgatttccag tttcttcact
     6361 cgcccaatac aataatggaa gattcatgca acaatccatg atcattctac cttgttgtga
     6421 aggatcacta ggatctcccc aagcttgtat aactcctgcc ttctcccaaa aacgctgaag
     6481 taatgtttct gcagcatcaa ttgaagcttc cttcattttt cgatttcccg ttattttata
     6541 atctgctacg gttgataaag aatacaaaaa accaagatcg tgatgattag tgataatttg
     6601 acagtcaatc ctgtggataa aatctatatc tgctttttca gctgtttgtt gaaataaagg
     6661 atcacccgtc ttctcaaata acaaccaatt aattcctgtc caaaatcctt ctgtccaacc
     6721 aatattgtct cttaatggat aacgtccttt tataggacac tcgcttggga attgatactg
     6781 actgttactg atacgtttga ttaacctctc cgtgtgtctg cgacaacgct cgatctcatt
     6841 attccattct ttttccatta attctttata acattcattt atccattttt ctggatgtat
     6901 tatctcttca aacatcactt tatcaagcat ttccccacca gcctttagca taattttagt
     6961 ctttttttat tgtttttatt ttggatagtt agcccggttg tcccatcaaa atcaattttg
     7021 ggacgattgt tccatacttc taaactataa tcccgatctt gatgagcaac cactcctgtt
     7081 ccgagccaat gggttccttt tccaaatgta ccctctaatc caggaataaa agtgttttcc
     7141 catgtcatca agtttgtatt gggcaaaccc ataatagctg tggattttcg tttaatagta
     7201 tcgtcactta aacagataat acctgaaaag ccagcttcac tcaccctata gttttccttg
     7261 tcttcttcta tgattattcc ttctttctca ggagaacatt caatagcaaa ccctgtctca
     7321 tatagaacat attctgtatc taattctact ttgtgtacgc gaatctgcca gccttcaaaa
     7381 ggaataagcc aagttctgat ttttgcctgt aaagtaggtt cccatgtaga ggcaagtacc
     7441 tctttcttac atacaatgtc tcgtacatta tttcgaacga tgaactgtcc ataggttttg
     7501 atgctctcat tcttaaaact ggtcgtgata tatgtgtccg ctcgctgaat ccctagcatt
     7561 gaatccattg ccatttctgc tttcaattga ttcgctcgcg gtatcgaaaa accaaattga
     7621 ctagaataag caaattttaa atatttttca ttggtcaatg tatggaaatt tggccctaat
     7681 tgtcccgcat tcaaaaaaaa tgtatgccca ttatcatgtt gtatgagcaa attggcttct
     7741 gaaagaagtt tagttccttc acgaataggc ataggcattt ctttagccgt ccaatactca
     7801 tggtcttcag gtagcgccaa caataaaaaa atcttattca gccataatgg agataacgta
     7861 ctattatagg gttcagtcaa tgtcaattga gggtaggtat aaccaatatt caggatacct
     7921 ttttcatcaa atatttcttt ttgcatccac catcttatat tccgattgac taaccccttg
     7981 atttcaccta atttaaaagg caaaagatcg gcccacaaca tagctgacca aaagcttgaa
     8041 actgcaaaac gataaatcat gcttcgacca aaaggcacat ttgcaccatc ttccgcaaaa
     8101 aaatgaatat agtctctaga aaaatcaata gctcggttct tatatatctg gctaatgggc
     8161 ttttctggtt ctagtacgct ataaatcaaa ccataataat gaaatgcaaa aggattataa
     8221 taatcttggc gacctcttga actgtctcga taccagccat tttgaagata acaattttct
     8281 atattatcaa atgcttcttt cagtgctgat tctttaactg gataaccaaa tttttggata
     8341 attgttccga caattatctt aaaaaattgc caattaccat ctgcacattc atattcaaaa
     8401 atttgcgaga accaatgaat aatctggctt ttttctcctg tatctaattt tttccataat
     8461 aattgttcat gatgatataa cgctaataac attgcgggca tttctaccat acgctggtct
     8521 ctttcatgta tttctcccca ataatcggga tgggaaggat ctgttccagc ttttattcct
     8581 gatagaatta atttcaacca tcgttcatct aattggtcaa ttgcgggccc cgctccccat
     8641 aaaacccgag agaatccttc cattcctgca attttatttc catatcctac cccttgatgt
     8701 atataatcaa tttgtgcgcc acttgctcga taaaacggct ggagtggttg atttatcttt
     8761 ttaaataagt caaagtaatc ctgtctggtc cttaatagtg gtactggatg tttcattgta
     8821 ttttctcctc tttttactac caaatagcta attccgactg ttctaatcga agtaagcctt
     8881 caataaaaaa ataatcagaa tagatcagtg gcacattata agtcagttct ctatggtatg
     8941 cttctgctcc gcctccaatg atcccatctt catccatatt ccaatttgca tagtgctttt
     9001 ctgtcttttg gagtatattg atagcagctt catgatacat ttttgatcgt acagaggaaa
     9061 catatcctgc taattccaga agaccgcatg cagcgcatac tccagcactg gtatcaatat
     9121 tcgaatcttt tacagcaaaa tcaatgagag gaatataacc agttttttcg attttctgaa
     9181 taaaatttac tgctatcttt tctgctatag ctagatagta cggatttttt gtgtaacgat
     9241 aacttaacgc gaatccatat aaagcccatg catttcctct gctccaacaa gaattctcat
     9301 tgtatccttg cccgccaagt aaccgaatat atttccctga ttctgcatca aattctgcga
     9361 tatgcccggt tgatccatct ttcctcacta gatacttagc tgttgtatct gcatgctgcc
     9421 gagcaatttt agagaacctc ggatcaccag tttccctcga agcccaatac agtaatggta
     9481 aattcatcat actatcaata atcatccaac caggtttatg gtcattccat gctactaaat
     9541 agttccctga aatattatag cgtccagcaa gtaccgtagc tgcatgtaac cctagttgtc
     9601 gactatgttt gttttgagac tgttgataat gtgcaatagc cgatggtaag aataaaaaac
     9661 ctacatcatg atgaacgtct gaaaaatggt acagggcaga ttctaataat tgttcttgtt
     9721 gtattgctgc ctctttgaac ttttctcgtt ttgtcttgtg aaagagctgc cataaaatac
     9781 cactccaaaa accattggtc caccagtcga ttcctctgtc ctttattacg tctgtatatt
     9841 ttccgttgta agaaatatat ggaattttat tccccattct gtctaattcc gcatctatct
     9901 tttcagaagc tacattcaaa atttttcttg accattcctt cgtctgttca ttcatggata
     9961 tcccgccatt cttttaattt ttatgaatgt ggagttgctc ctagaacgaa ctagatggtt
    10021 aacattcgtt atttttttta cttatttgca gctccaatgt cattctcctt aaatttagac
    10081 tgcgcccaca cataaatcaa gaccattggg atcactgaag ttgctaaaat tagcataatt
    10141 gcgccataat taattggctc acctgtacta gaaaccgtca tatttttgat aattaaggga
    10201 accgtatatt tatctttatc tgaaagcatg actaatggaa atagatattg attccaactt
    10261 cccataaaga taagtaaacc actagtaata atcgtaggtg ataaaatcgg tacagcgatt
    10321 ttcgtaaaaa tcacccactc attggcacca tcgattctgg cagcctccat cagtgctgta
    10381 ggaaattgac tgctatattg tcgcatcata aacactgaag ttgtactggc aatcgaaggg
    10441 atgatcacac ctgcataagt attcgataat cctaaacgac tcataatgta aaataaagga
    10501 atcaatgtgg taaaaaaagg caccatccta gatagcataa ctgtattgaa caacagttta
    10561 ttccccataa aacgatactt tgctaatgca taacccgcca ttgtagaact ggctgtagct
    10621 aaaactgtac tgattacagc aataacaatc gaattccaaa taatcaaggg ataattatat
    10681 ctcgccatga tggcttgcca gttttttaat agcatatccc caaacttgaa attgattccg
    10741 ccacttaaaa tttcttgatt actttgcgtt gaagctacaa tcatatataa atatggcatc
    10801 aaaaatatta aggcaccaaa cacaataatg acgtacgtaa aaattctgct ttgtatcttt
    10861 tgtttagtca tttttatcac ctccgaattt aaattgaata gccgataata taacagatac
    10921 aaacaccatc gtccaggcaa tagcagaggc ttttccaaaa tcaaccattt caaaacttgt
    10981 tttatataaa ttgactccta cgaccaaagc tgaattttct ggtccattgg aagtcccaaa
    11041 gaatatattt gggatattga aattacccaa gccattgata gtggctaaaa ctgtggccat
    11101 taaaatgatt ggctttagca tggggatcgt taataaaaag aattgccgaa atttcgatgc
    11161 tccatctatt tcagcacttt cgtatatctc tttagaaatc ccttgaagac cagctaaaaa
    11221 taaaatagtg ttatatccta cactaagcca aatcatcgtg atgatcattg atattcgtat
    11281 ccaaaaagga tttgttaacc actgtacacc gttaattcct agcgcattca aaaatccatt
    11341 gaataaccca gtcggattga aaaaagtcat gaatacggaa gctacagcca ccgttgatgt
    11401 aatggttgga atataataga cagttctaaa aaatcctttt ccttttaact gtgctgaatt
    11461 caataaaaca gcaaagtaaa tagctaatag catcgttatt ggaattgatc ctgccatgat
    11521 aatcaaaaca ttcgtgtaag atttaataaa caatggatct cggaagatag ctagataatt
    11581 gtcaaatcca acaaattcaa atgagtagcc aattttcata aaactaaaag caaacgagat
    11641 aatgatggga atcaaaatcg tcacaataaa gaaaataaca aaaggtgtga ccaatccata
    11701 agcacttttt ttatgagacg tatctgttgc ttttttcttg ctgttcttta ttgtcttgct
    11761 tggtaacgaa gattgcatca tcgttaagtc ctcctaatat tttttaatca ccagttaatt
    11821 ttacgccgta cttatgagca aaattttctg cttcttttag atatgaattt tcaaaatcac
    11881 ctgtttgcca atagttatat gcagcggtag tcaaataaga cgtagcatct gaataagtgg
    11941 ctggtaaaac agtatttgat gtattttcat tgattttatt caatagttca tagtattttt
    12001 gtcctcctta gtagtcaaac tttttctcag ctgcttctgt ttgataggcc aatgagttag
    12061 atgtagccac gcccagctcc agtgcgttgg ctaagcattc ttcatctgtc agcataaatg
    12121 taagaaattg ttgtgccact tgtggatttt tggcatcttt agggacataa aaacttgatc
    12181 ctcctgatac tgcacctttt cctggatttt cttttgaaaa aggaatcagt ggagccatgc
    12241 gccatttgtc agatgcatca ggaaaattca aagacatatt cgtagcatac cagccgccag
    12301 caacaaacat tgcagattct tgaaatgctt tctcagcatc ttgtgcaccg tagaaatgta
    12361 caatatctga gtcgatcatt tctttgatta actttgcagc atgtttcgct tctttagtat
    12421 tcagacggat atttccttct tcatcaagca aagattgatt ctgctgggct agcatgttga
    12481 taaacaattc ttggtcccca gattctggca aggcaattcc aaaactacca gttttttctt
    12541 ttaatgcttt tgccacctgg atatattctt cccaagaact gatttcttca aaatctacac
    12601 ctatttcagc aaattttttt ggttgataat aacaaagtgc tggggtaaaa tcactagccc
    12661 aaggaataat cgtttcatta ctcgtttgtt gacgcaaaat attcaatttt tgatcataaa
    12721 actgatcgcc atatttgtca taaaatccat attcatcagc actataaaaa gcatcttgaa
    12781 atttttccag cacacctgct gctgaagtgt ctgccatgga gataatatcc ggcatatctt
    12841 cgtgtgttac caattttggg gtgatcattt gtactgttgc tgttcccgaa gctacattca
    12901 caatttttaa gtcaatattt gctccagttt tgcggttata gatttttgca gcattttcaa
    12961 catagtttgt cagtttgccg tttgcgacat atacttctaa tgtagtggct ttttttatca
    13021 ttggatctgc gatgtttgct gttacttctt cgttagtttt tccacatgca cttaagctga
    13081 ataatagggt aatgtttagc aaagctatca ccaatttgta tctttttttc atgtctatcc
    13141 c
//
LOCUS       DACDKL010000069        13037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDKL010000069 DACDKL010000000
VERSION     DACDKL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.069"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..266
                     /locus_tag="IV329_001487"
     CDS             <1..266
                     /locus_tag="IV329_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8940669.1"
                     /translation="IILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAII
                     FGFWGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            784..1239
                     /locus_tag="IV329_001488"
     CDS             784..1239
                     /locus_tag="IV329_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8940670.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            1550..1906
                     /locus_tag="IV329_001489"
                     /pseudo
     CDS             1550..1906
                     /locus_tag="IV329_001489"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1919..2329
                     /locus_tag="IV329_001490"
                     /pseudo
     CDS             1919..2329
                     /locus_tag="IV329_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            2360..2608
                     /locus_tag="IV329_001491"
     CDS             2360..2608
                     /locus_tag="IV329_001491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HAP8940671.1"
                     /translation="MQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITENEF
                     AYLKANFGKYSGFLGLDLEMLLSLIMIRSNNSTQILTN"
     gene            complement(2759..3738)
                     /locus_tag="IV329_001492"
                     /pseudo
     CDS             complement(2759..3738)
                     /locus_tag="IV329_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3743..4510)
                     /locus_tag="IV329_001493"
     CDS             complement(3743..4510)
                     /locus_tag="IV329_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8940672.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            4999..5424
                     /gene="lacA"
                     /locus_tag="IV329_001494"
     CDS             4999..5424
                     /gene="lacA"
                     /locus_tag="IV329_001494"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8940673.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            5441..5956
                     /gene="lacB"
                     /locus_tag="IV329_001495"
     CDS             5441..5956
                     /gene="lacB"
                     /locus_tag="IV329_001495"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8940674.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            5967..6899
                     /gene="lacC"
                     /locus_tag="IV329_001496"
     CDS             5967..6899
                     /gene="lacC"
                     /locus_tag="IV329_001496"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8940675.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            6902..7882
                     /gene="lacD"
                     /locus_tag="IV329_001497"
     CDS             6902..7882
                     /gene="lacD"
                     /locus_tag="IV329_001497"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8940676.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            7910..8227
                     /locus_tag="IV329_001498"
     CDS             7910..8227
                     /locus_tag="IV329_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8940677.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            8227..9933
                     /locus_tag="IV329_001499"
     CDS             8227..9933
                     /locus_tag="IV329_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8940678.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10075..11481
                     /gene="lacG"
                     /locus_tag="IV329_001500"
     CDS             10075..11481
                     /gene="lacG"
                     /locus_tag="IV329_001500"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8940679.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            11504..11776
                     /locus_tag="IV329_001501"
     CDS             11504..11776
                     /locus_tag="IV329_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP8940680.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            11797..12696
                     /locus_tag="IV329_001502"
     CDS             11797..12696
                     /locus_tag="IV329_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8940681.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4190 a         2203 c         2557 g         4087 t
ORIGIN      
        1 gaatcatttt aagttacagc caatatttgg acaaattgga aaatatgata aacaatatga
       61 tttcgtatca gctaaacatg attatgaaaa ccctaaccga gatatcaatt gttttgacta
      121 ttccagcaat tatttttggc ttttggggaa tcaatgtcca tgtcccgttt gaaaagtctt
      181 cttatggtgt tttagccgta ttgattatct ctgctatact aagtctaatt tgctggcttt
      241 tgatgcgacg caaaacatat ttgtaaagta tttgtgatac cctaatatga tttaaagcag
      301 aatttctttg ttgacttttt aaaaggctga ttcctttcaa taatttaata gcttcagcgg
      361 ctgctcagaa gtcaataaat tggactattt ttattcagtt acttaggcaa catttgagat
      421 acgattgcga ttataaaaat tgatatacca accaatggct gcttggaaga ctgacatatt
      481 agcctcgata tcatcgaata actaaataca gaaaaataca ataaatatta tcaaactagg
      541 gactatacta gaaaacatca gtacattcct taactcaggg tctataatta gaaaatcgcc
      601 cctttgaaaa ctgattaaaa cagcccccat tatctagtga ctagataatg ggggctgttt
      661 ttttgttaag tgatataata aaccataaaa gaagtttaga aatacggtcc tggtcatcaa
      721 taaataaaca atttaattaa ttatttattt aattatttaa tttcagaaag gtatacatga
      781 atcatggtgc aacaaattgt cctacccatc aaagactcca gcgtcttaaa gatggtacaa
      841 gatacacttc tcgatagttt ccgtgctggt cgccgaaatt acaccgtttt tcaagttggt
      901 aaggccacgc tactacgtgt gagtgatgtc atgacattaa aaaaatcaga tgtctataat
      961 ccagacggct ctgtcaaaaa tgcaaccttt attcatgata aaaagaccgg taaagcaaac
     1021 acgttatatt taaagcctgt tcagcaagac ttgttgcaat atcatgattg gctggcccaa
     1081 gaaaatatca attctgagtg gttatttccc tcgacagccc attatgatct tcatatcact
     1141 gagaagcagt ttcataaggt gatggcgcat gttggtgatc tcttaggaat caacttggaa
     1201 gacaaagaga aaaaattgcg agtcagaaaa ccgttttgag atatgaacag catttgcctt
     1261 cacgccttag cgattttaaa taatagcctt gaaagtgatt atggaaaagc cctctacgct
     1321 caatgcaaaa ttgacgtaga aactattttt gatcgattga aaggtgtatt tggtatgtga
     1381 agaacccatg tcagaggcaa gaacgttgca cctactgaca ttggaatgat gcttatgagc
     1441 atgaatctaa ccaaattatc ccttgaggct agaagaaaag cggaagcttt tcaacacaaa
     1501 gcagccaaaa ataaaaaacg cgacgaagca atcacattta tgattattca tcgcgttttt
     1561 tatatttaga gctagttatt tgctagaccc actatttaca gatgaaacgt ttatcacctt
     1621 tggttttggg catttagtgg aattagcaga accaggtcat tatgacgaaa agtggcaaaa
     1681 ttggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     1741 taagggaaag cagtttaaaa ttgttgcaga acttctcaaa aaggcaaata caattattgt
     1801 tgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataatgcaaa
     1861 tgccttttca aaagataaaa catttaaaag actatggatc aatagcttag aaaaagatgt
     1921 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     1981 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttgtatac
     2041 gttaaattta caacagaagg gcgtacaagg tacattttca ctaggacgtg ttcaaacgcc
     2101 caccttatac cttatttttc agcgccagga agccatagag aattttaaaa aagaaccttt
     2161 tttcgaggtg gaagctaagt aaaacaaggg tcatttaagg gcgttctaag ccccacacag
     2221 cgttttaaaa cccaagagga gctttttagc ttttgtttct tctaaacaag ctaaaatagg
     2281 caatcaagag gggataattg ctgatgttca aaccaaagag aagaaaacga atagtccgag
     2341 ttcgttttct ttaagtagtt tgcaatcaaa agtcaatcag ctttataaag cgacagcgag
     2401 ccaaacttta aaagctatgc aaggactgta tgaagcaaaa ttattgagtt atccaagaac
     2461 agatacacca tttattacag agaacgaatt tgcttattta aaagcgaatt ttggcaaata
     2521 tagcggtttt ttaggacttg atcttgaaat gttgttatca ttgataatga tacgttcaaa
     2581 caacagcacc caaattttga cgaattagtg aaactctatg aaaaagacgt agtaaaacat
     2641 gcaacatcct attctaatca gctggtaaag cttaattagg ttatataagc ttgaaaataa
     2701 agggtttgtc caatatggac aagccttttt cttatgtttt gccacagatt tgccacactt
     2761 attgtgtaag agtatcaagg ataggtataa tttgatactt tgttttttta gtgatgtgca
     2821 aataaatttc tgttacacga ctacctctag aatgccctac acggtcttga attgcttcta
     2881 ggggtattcc ttactctgct aagaaactaa tatgcgtatg acggaatata tgggtcgtaa
     2941 tatgtttatt aaaactaagt ttattatcaa tatcaattcc tgctcgtttt ggattacctt
     3001 ttcgatttaa ttttccattt tcaggtgagt tgaggaaatt gttaaggaca ctataatgaa
     3061 gaggtgttcc attatagtcg ggtttatgac gacttttgga agtaaaaatc catccattat
     3121 ctttataatc aggattgaat ctcatatgtt ggtgatttaa ctcaatttcc tctttaagaa
     3181 tttctatagc ccttttagat aattggacat ttcggtaact ttgtaagttt tttacattac
     3241 cttcatagct attttctgca gctcctccgt gagtggactg actaatgatg atattttgct
     3301 tttcaaaata ttgataggca ccaatatttt ggagtttcga ctctgtaagt caacagggcc
     3361 attagttaga atacctattg ggatgcggtt tttatgtaga gccagcagta gttgatgtat
     3421 ctcaggaatc atcgtaatct gatcgagttc atattcatag tcagcttgga agtctagagc
     3481 ttctgtccta gaaatactat cgatgccaaa ttcttccata gtcttctgac agcgaaaaat
     3541 tctaagctgc tcaatactga tgagtttttt cgtgtattta ggaaaagcaa catcagacca
     3601 atatctgaaa cgtttgtaaa tgttatctat ttgttggata tcaatcgttg gaaaacattt
     3661 ctcaacgcta cgtttaaagg ggagttgatg gtcataaaga gtgtcatcaa ggtcaaaaac
     3721 aatattttta taagtcataa ttctagtccg cttttaaaat agtagtgtac tggctaaatt
     3781 cctctaagtg ttgagggcta atctggttat ctgtaatgat ggtatcgagt tgatctagat
     3841 tatagaagtt aaagaaatcg tatcgatcga atttagtact gtctactaat aagaattttt
     3901 ctacagcatt gtttagggca agttgttgaa tcacaccttc cttgtcacta tatgtagcaa
     3961 tagaattatg ggtaacagca tttgcactaa caaaagcttt ggcaaatcta attgctttta
     4021 aatttgtcga agccattgaa cctacaaaag ctccagttat ttctctatat tcaccgccaa
     4081 gaagcaataa atcaatggtt tcgctatcat ttagaatcaa gaacacaggg agactatttg
     4141 taatgacacg aattttataa cctttacgac ctttcaattc taatgccagt tgttcaagtg
     4201 tagttccagg tccaataaaa atagtatcgc catctttgat taaagagcag gcttttttag
     4261 caatgtcaat tttttctttt gtatttagac ttttcttctc gatatgtgtc ttttcaagtg
     4321 gctttttaga ggaaagcaat tgtgcaccac catgagtacg tgttaaaagt ccatcagctt
     4381 ctagagcatc aaggtctcta cgggctgtca tatcggaaat atctagttca tctattattt
     4441 cttttatggt tatggtccca tcaatcttta acatatctaa aattttttct aatcgtcgtt
     4501 ttttgttcat atgaagaccc ttctttcata aagtaatttt tttccaaaga taattctctt
     4561 ttaattgtat cataaaagac aatattttca aggtaaaaca aacaatttca aacaaaaaca
     4621 aatgttagat gatgaaataa gaacagagga ttgacgtata ttagcttagg tcagattttg
     4681 tataagacga aaataaagta ggacctctta atcagtaagt tatagaaagt aaaagacttt
     4741 tgtaatactt gaatagatat ttcacgtcca ttttgtgatg gattaaatga acaaaaatga
     4801 acaataattt aacggtgtta tctatttttt aaaaaaacaa ataaaaaaac aaaaaataaa
     4861 caaaaaaagt gttgcttttt gtttgaatgc ttgatattat ataaacaaag aaatgatgaa
     4921 aacgtttttt cgaacatttt gcaaaatgtt ttctacttct acgtagcatt tctttctaaa
     4981 atttaggagg tggtcaaaat ggctattgtt gttggtgcag atctcaaagg tacgagatta
     5041 aaagatgttg tgaaaaattt tcttgtagaa gaaggttttg aggtaattga tgtgactaag
     5101 gacggacaag attttgttga tgttaccttg gcagttgcct ctgaagtaaa taaagatgag
     5161 caaaacttag gtattgtaat tgatgcttat ggagctggtc cattcatggt agcaactaaa
     5221 attaaaggta tggttgcagc agaagtttca gatgaacgct cagcttatat gacacgtgga
     5281 cataacaatt cacgaatgat tacggttggc gctgaaatcg tcggtgatga gcttgctaaa
     5341 aacatcgcta aaggtttcgt caatggtaaa tatgatggcg gacgtcatca aatccgagta
     5401 gatatgctta acaaaatgtg ttaagaaaag gagttaagga atgagaatcg caattggatg
     5461 tgaccacatt gtcacagatg aaaaaatggc agtatcagaa ttcttgaaat caaaaggaca
     5521 tgaggtcctt gatttcggga catatgacca cgcacgtact cactacccta tctatggtaa
     5581 aaaagtcggt gaagcagtag taagtggtca agcagacttg ggagtatgta tctgtggtac
     5641 aggtgtcggc attaacaatg cagtcaataa agttccaggt gttcgctcag cgttggttcg
     5701 tgatatgaca tcagctatat atgcaaaaga agagttaaat gcgaatgtta tcggtttcgg
     5761 tggaaagatt actggtggtc ttcttatgaa tgacatcatt gaagctttca ttgaagctga
     5821 gtacaaacca acagaagaaa ataaaaaatt gattgcaaaa atcgaacacg ttgaaacaca
     5881 taatgcacat caagcagatg aagatttctt tacagaattc cttgaaagat gggaccgtgg
     5941 tgactaccac gattaaggta taacaaatga ttctgacagt cacactaaat ccttcagtag
     6001 acatctctta tcctctagaa acattgaaaa ttgatacagt gaatagggtg aaagatgtta
     6061 gtaagacagc tggtggaaaa ggcttaaatg ttactagagt cttgtatgaa tctggtgata
     6121 aagttactgc tacaggtttc ttaggtggta aaattggtga atttattgaa agcgaattag
     6181 aacaatctcc tgttagtcca gctttttata aaatctcagg aaacactaga aactgtattg
     6241 ctatcttaca cgagggtaac caaacagaaa ttttggaaca aggaccaaca atttctcatg
     6301 aagaagcaga aggctttctt gaccactata gcaatcttat taaacaatca gaagttgtga
     6361 caatttcagg aagtttacct tcaggacttc ccaacgatta ttatgaaaaa cttatccaac
     6421 ttgcttcgga tgaaggagta gcagtcgttt tggattgttc aggtgcacct cttgaaacag
     6481 ttctaaaatc gtcggctaaa cctactgcca tcaaacctaa taatgaggaa ctttcacagt
     6541 tgcttggaaa agaagtaaca aaagatattg aagaactcaa agacgttctt aaagagtcac
     6601 tttttagtgg tatcgaatgg atagtggtct cattaggtcg aaacggtgcc tttgcaaaac
     6661 atggtgatgt tttctataag gtagatattc ctgatatccc agttgttaat ccagtcggat
     6721 caggggactc aacggtagct ggtattgcat cagctttaaa tagtaaaaaa agtgacgctg
     6781 acttattaaa acatgcgatg acattgggta tgttaaatgc tcaagaaaca atgacagggc
     6841 atgtgaatat gactaattac gaaacactaa actcacaaat tggagtaaaa gaggtataaa
     6901 aatggtactt acagaacaga aacgcaaatc tttggaaaaa atttcagata aaaacggtgt
     6961 tatctcagct ttggcatttg accaacgtgg tgctttgaaa cgtttgatgg cacagtatca
     7021 agatacagaa ccaactgtag ctcaaatgga agaactaaag gttttggttg ctgatgaact
     7081 tacaaaatac gcttcttcaa tgttgcttga ccctgaatat ggacttccag caacaaaagc
     7141 attggataaa gaagcaggtc ttcttcttgc ctatgaaaaa acaggttatg atacatcaag
     7201 tacaaaacgt ttgcctgact gcttggatgt ttggtctgca aaacgtatca aagagcaagg
     7261 cgctgatgcc gttaagttct tgctttacta tgatgtagac agctcagatg aacttaacca
     7321 acaaaaacaa gcttatattg aacgtgttgg ttctgaatgt gtcgctgaag atattccttt
     7381 cttcttggaa atccttgctt atgatgaaga aatctcagat gcaggttctg ttgaatatgc
     7441 aaaagtgaaa cctcgcaaag tcattgaagc gatgaaagtt ttctctgatc cacgctttaa
     7501 cattgatgtt cttaaagtag aagtaccagt taacgttaaa tatgtcgaag gttttgctga
     7561 tggtgaagta gtttacagta aagctgaagc tgctgacttc tttaaagcac aagaagaagc
     7621 gactaacctt ccatacatct acctaagtgc aggtgtatct gctaaattgt tccaagaaac
     7681 attgcaattt gctcacgact caggtgccaa gtttaatggt gtgctttgtg gacgtgctac
     7741 ttgggcagga tctgtagaac cttacatcaa agaaggtgaa aaagcagcac gtgaatggtt
     7801 gcgtactact ggattcgaaa atattgatga acttaacaaa gttcttgtta aaacagctag
     7861 tccatggact gataaagtat agtaaaaaca taaaacggag gatattgtta tgaacagaga
     7921 agagatgact ctcttagggt ttgaaattgt tgcttatgct ggagatgctc gctctaaact
     7981 tttagaagcg cttaaagcgg ctgaaaatgg tgatttcgct aaggcagata gtcttgtagt
     8041 agaagcagga agctgtattg cagaggctca caattctcag acagctatgt tggctcgaga
     8101 agcttctggg gaggaacttc catacagcgt taccatgatg catggtcagg accacttaat
     8161 gactactgtc ttattaaaag atgtgattca tcacctcatc gaactttata aaagaggagc
     8221 aaaataatgc ataaactcat tgaacttatt gagaaaggga aaccgttctt tgagaaaatc
     8281 tctcgaaaca tctatcttcg tgctattcgt gatggattta tcgctggtat gccagtcatc
     8341 cttttctcat ctatctttat ccttattgcc tatgtcccaa atgcttgggg attccactgg
     8401 tcaaaagaca ttgagacctt cttgatgact ccttatagct attcgatggg tatcctagca
     8461 ttctttgttg gtggtactac cgctaaagct ttgacggact caatgaaccg tgacctacct
     8521 gcgaccaatc agattaactt cttatctacg atgctagcgt ctatggtagg gttccttttg
     8581 atggcagctg agcctgcaaa agaaggaggc ttcttgacag ccttcacggg aacaaaaggt
     8641 cttttgacag catttattgc agcctttgtg acagttaatg tttacaaggt ctgtgtgaaa
     8701 aataatgtta ctattcgtat gcctgaagag gttccaccaa atatctctca agtatttaaa
     8761 gacttgattc cattcactgt agcggttgtt ctcctttatg gatttgaact cattgttaag
     8821 ggaactcttg gtgtaaccgt tgcagaatca atcggtaccc ttcttgctcc tctcttctca
     8881 gctgcagatg gttaccttgg aattacactt atctttggtg cttatgcctt cttctggttt
     8941 gttggtatcc acggtccttc aattgtcgaa ccagcaatcg ctgctatcac ttatgccaat
     9001 atcgatgcca acttgcatct tatccaagct ggacaacatg cagacaaagt tatcacttct
     9061 ggtactcaaa tgtttattgt taccatgggt ggaacaggag ctacattgat tgttccattc
     9121 ttgttcatgt ggatttgtaa atcagaacgt aaccgtgcca tcggacgtgc ctcagttgtt
     9181 ccaacattct ttggggttaa tgagccaatc ttatttggtg caccaatcgt attgaatccg
     9241 attttctttg taccgttcat tttcgctccg attgtcaacg tttggatctt taaattcttt
     9301 gttgacacat tgaacatgaa ctcattctct gccaaccttc catgggttac tcctggtccg
     9361 ttgggaattg tactcggtac taacttccaa gttctatcat ttatcttagc cggactcttg
     9421 gtagttgttg atactatcat ttattaccca tttgttaagg tatacgatga acaaattctt
     9481 gaagaagaac gttctggtaa aacaaacgat gctcttaaag aaaaagtagc tgcaaacttc
     9541 aatactgcta aagcagatgc cgttcttgga aaagcaggcg ttgctaaaga agatgtagca
     9601 gcaaacaata atatcacaaa agaaactaat gtccttgttc tttgtgcagg tggaggtaca
     9661 agtggcttgc ttgccaatgc cttgaacaaa gcagcagcag aatacaatgt tccagtcaaa
     9721 gcggcagcgg gtagttacgg tgctcaccgt gaaatgttgc cagagtttga cttggttatc
     9781 ttggctccac aggtcgcttc aaacttcgat gatatgaagg ctgaaacgga taaattgggc
     9841 attaaacttg caaaaactga aggagctcaa tatatcaaat tgacacgtga tggacaaggt
     9901 gctcttgcat ttgttcaaca acagtttgat taataaattt gtaaaagtta tttgagacga
     9961 gattattctt tcaattcctt ataatattgt taaccatatc attgcatttc atttccttgg
    10021 tttccggaac tcagaatatc gtctcaaatg ctttttttga aaggatttat agttatgact
    10081 aaaacacttc ctaaagattt tatttttggt ggtgctacag ctgcttatca agcagaaggt
    10141 gcgactcaca cggatggtaa aggcccagtt gcatgggata agtatcttga agacaattat
    10201 tggtacacag cagagcctgc aagtgatttt taccataaat atcctgttga tttagaatta
    10261 gctgaaaaat atggcgttaa tggtattcgt atttcaattg cctggtctcg tattttccca
    10321 actggctatg gcgaagtaaa tgaaaaaggt gttgaattct accataatct ttttgccgag
    10381 tgccacaaac gtcatgttga gccattcgtg actttgcacc actttgacac accagaagtt
    10441 cttcactcag atggagattt cttaaaccgc gaaaatatag aacactttgt agattatgcc
    10501 gctttctgtt ttgaagagtt cccagaagta aactactgga caactttcaa tgaaattggc
    10561 ccaattggag atggtcaata cttggttggt aaattccctc caggcattca atatgatctt
    10621 gccaaagtct tccaatcaca ccacaatatg atggtttctc atgcacgtgc tgtgaaattg
    10681 tataaagata aaggttataa aggcgaaatt ggtgtagtac atgcgttgcc aaccaaatac
    10741 ccttatgatc cagaaaatcc agcagatgtt cgtgccgctg agttagaaga tatcatccat
    10801 aacaaattta tcttggatgc aacctacctt ggacactatt cagataaaac gatggaaggg
    10861 gtcaaccaca tcctagctga aaatggtgga gaacttgatc ttcgtgatga agacttccaa
    10921 gcacttgaag cagctaaaga tttgaacgat ttccttggta tcaactacta catgagtgat
    10981 tggatgcaag cctttgatgg tgagactgaa atcattcaca atggtaaggg tgaaaaagga
    11041 agttctaagt accagattaa aggtgttggt cgccgaatcg caccagatta tgtgcctaga
    11101 acggattggg actggattat ttatcctgaa ggcttgtatg accaaatcat gcgagtgaaa
    11161 aatgattatc caaactacaa gaagatttac atcactgaaa acggtcttgg atacaaagat
    11221 gagtttgtag ataataccgt ttatgatgat ggtcgtattg attacgtgaa gaaacacttg
    11281 gaagttttat cagacgcaat tgcggatggt gcaaatgtta aaggttactt catctggtca
    11341 cttatggatg tcttctcatg gtcaaacgga tacgaaaaac gttatggact attctatgta
    11401 gactttgaca cgcaagaacg ctatcctaag aaatcagctc attggtataa gaaagtagca
    11461 gaaactcaag tgatagagta atattacttg aaaatgtaaa aaattgttat ccgatacttt
    11521 agaaaaagta caacacttat ttagaccagg ctttgaaaag gtatatattg ttagtttcga
    11581 agattgtcct atgattcctg agcttgaagc taccccgttg ctaaaatgtg gtaaatggta
    11641 tgtttcaact ggaaaagagt ggatttgcca ttctgattta gagttatcag catttgaatg
    11701 ggaattttta caatcactcg atgtagaaat acgtgagact attcacttcg aggtaaatta
    11761 tttgcctttc caataataaa aagaaaggaa ttagttgtga ctatcgaatt aaaaaacgag
    11821 tatcttactg ttcagtttaa aacacttggt ggtcaactga cttcgattaa agacaaggat
    11881 gggatagagt atctctggca agctgatcca aattattgga atggacaggc gcctattcta
    11941 tttcctatct gtggtagttt acgaaatgat tgggctattt atagacctca agaaagaccc
    12001 ttttttacag gtcttattcg tagacatggt tttgttcgaa aagaagaatt tattctggaa
    12061 gaagtcaatg ataatagcgt gacctttagt attaaaccaa atgctgagat gcttgataat
    12121 tacctatatc agtttgaact gagagttgtt tataccttga atggtaaatc aattagaacc
    12181 gaatttcagg taacgaactt ggaaatggaa aaaacaatgc cttactttat tggagcccat
    12241 ccagcattca atagtccttt agtagaaggt gaaaactatg aggattatta tattgaattt
    12301 agtgaggttg agtcttgctc gataccaaag agttttcctg agacaggatt attagatttg
    12361 caagatcgga cacctttttt ggaaaatcaa aaaattctcg atttggacta ctcacttttt
    12421 tcccatgatg ccattacact tgaccagctc aagtctcgta gcgtaacgct tcggtcaagg
    12481 aagtctggta aaggtcttcg agtagatttt gatgattttc cgaacttgat tctgtggtct
    12541 acaactaata aaagtccatt cattgctttg gaaccttgga gtggtctctc cacttcactt
    12601 gaagaaggta atttcttaga agataaaaga caggtaacga aaatttcacc tcaagaaact
    12661 tctagaaaaa tctatgatat tactatcctt aattaaaatt tgttattatt tgagtcttca
    12721 ataagatttg aatactctta ccttgtgatg atagattggt cttctccgtg ccagtcttac
    12781 atcgcaaggt ttttaagttt tttgtgggtc tatgtcaact gtagtgggtg acgaaaagct
    12841 aatatctaaa gacagtctgg tagtaaaaaa taagattatt tactacttct tgatttaaaa
    12901 taatagtgga gccattaatt tgtggcaaat ttaatttaat attttgcctc aaattagcca
    12961 cactttttat agaactaaga ggaaaaatga aattttcttc ctattaatat agttgtttga
    13021 aatcttaaat atagcgt
//
LOCUS       DACDKL010000070        12961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDKL010000070 DACDKL010000000
VERSION     DACDKL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.070"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IV329_001503"
     CDS             139..477
                     /locus_tag="IV329_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940682.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IV329_001504"
     CDS             complement(682..1257)
                     /locus_tag="IV329_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8940683.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="IV329_001505"
     CDS             complement(1989..2570)
                     /locus_tag="IV329_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8940684.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="IV329_001506"
     CDS             complement(2753..3379)
                     /locus_tag="IV329_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8940685.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="IV329_001507"
     CDS             complement(3401..4732)
                     /locus_tag="IV329_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8940686.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="IV329_001508"
     CDS             complement(4887..5288)
                     /locus_tag="IV329_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8940687.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="IV329_001509"
     CDS             complement(5374..5580)
                     /locus_tag="IV329_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940688.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="IV329_001510"
     CDS             complement(5675..7177)
                     /locus_tag="IV329_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8940689.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IV329_001511"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IV329_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8940690.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="IV329_001512"
     CDS             complement(7748..8365)
                     /locus_tag="IV329_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940691.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="IV329_001513"
     CDS             8586..9959
                     /locus_tag="IV329_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8940692.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="IV329_001514"
     CDS             10034..11146
                     /locus_tag="IV329_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8940693.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="IV329_001515"
     CDS             complement(11218..12855)
                     /locus_tag="IV329_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8940694.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3506 a         2749 c         2311 g         4395 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 a
//
LOCUS       DACDKL010000071        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDKL010000071 DACDKL010000000
VERSION     DACDKL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.071"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV329_001516"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV329_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8940695.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV329_001517"
     CDS             complement(322..891)
                     /locus_tag="IV329_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8940696.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV329_001518"
     CDS             1077..1979
                     /locus_tag="IV329_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8940697.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV329_001519"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV329_001519"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8940698.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV329_001520"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV329_001520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8940699.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV329_001521"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV329_001521"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8940700.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV329_001522"
     CDS             8547..8732
                     /locus_tag="IV329_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8940701.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV329_001523"
     CDS             complement(8794..9990)
                     /locus_tag="IV329_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8940702.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV329_001524"
     CDS             complement(10160..11596)
                     /locus_tag="IV329_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8940703.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV329_001525"
     CDS             11889..12698
                     /locus_tag="IV329_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8940704.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDKL010000072        12515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDKL010000072 DACDKL010000000
VERSION     DACDKL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.072"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..720
                     /locus_tag="IV329_001526"
     CDS             <1..720
                     /locus_tag="IV329_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8940705.1"
                     /translation="IVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCCAPCSTH
                     TLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIVDEYRPN
                     EFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISPKKNAAL
                     INQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAKQQGCDL
                     KEINREAKAYLKTSTVVFEEN"
     gene            845..1324
                     /locus_tag="IV329_001527"
     CDS             845..1324
                     /locus_tag="IV329_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8940706.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            1321..2088
                     /locus_tag="IV329_001528"
     CDS             1321..2088
                     /locus_tag="IV329_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940707.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            2318..2887
                     /gene="lepB"
                     /locus_tag="IV329_001529"
     CDS             2318..2887
                     /gene="lepB"
                     /locus_tag="IV329_001529"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8940708.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(2991..3593)
                     /locus_tag="IV329_001530"
     CDS             complement(2991..3593)
                     /locus_tag="IV329_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8940709.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            3699..4168
                     /locus_tag="IV329_001531"
                     /pseudo
     CDS             3699..4168
                     /locus_tag="IV329_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            4274..4858
                     /locus_tag="IV329_001532"
     CDS             4274..4858
                     /locus_tag="IV329_001532"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8940710.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            4861..5934
                     /gene="prfA"
                     /locus_tag="IV329_001533"
     CDS             4861..5934
                     /gene="prfA"
                     /locus_tag="IV329_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8940711.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            5927..6766
                     /gene="prmC"
                     /locus_tag="IV329_001534"
     CDS             5927..6766
                     /gene="prmC"
                     /locus_tag="IV329_001534"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8940712.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            6759..6938
                     /locus_tag="IV329_001535"
     CDS             6759..6938
                     /locus_tag="IV329_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940713.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            6960..7985
                     /locus_tag="IV329_001536"
     CDS             6960..7985
                     /locus_tag="IV329_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8940714.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            8160..9404
                     /locus_tag="IV329_001537"
     CDS             8160..9404
                     /locus_tag="IV329_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8940715.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            9557..10504
                     /locus_tag="IV329_001538"
     CDS             9557..10504
                     /locus_tag="IV329_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8940716.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            10638..11267
                     /gene="upp"
                     /locus_tag="IV329_001539"
     CDS             10638..11267
                     /gene="upp"
                     /locus_tag="IV329_001539"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8940717.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(11406..11648)
                     /locus_tag="IV329_001540"
     CDS             complement(11406..11648)
                     /locus_tag="IV329_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940718.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            11769..11841
                     /locus_tag="IV329_001541"
     tRNA            11769..11841
                     /locus_tag="IV329_001541"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11802..11804,aa:Thr,seq:cgt)
     gene            complement(11960..>12515)
                     /locus_tag="IV329_001542"
     CDS             complement(11960..>12515)
                     /locus_tag="IV329_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8940719.1"
                     /translation="LFAPSIISIFSLTITGFIAFTLVFGKKDTKKMFSKPVAPVKNIA
                     KYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFLA
                     IFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKSN
                     SIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         4322 a         2128 c         2640 g         3425 t
ORIGIN      
        1 atcgtagaaa aaatgaaaaa tcaaaaaata aattatgata cagttctgaa aaaaatgatc
       61 atgcaatggg agaaagaaga agtaaagcct aagatattaa tacatacatg ctgtgctcct
      121 tgcagtacgc acacattgga attcatgtct caatatgcag agatcacgtt atttttttca
      181 aattcgaata ttcatcctaa aagtgagtat ttgcgaagat tacatgaaca aaaacgattt
      241 gtagaccaat ttaatgagaa aactgaccat catgttgcgc taatcgttga tgaataccgt
      301 ccaaatgaat tcaccaaaat ggttttaaca aaccatcttg aaaacgaaaa agaaggtggt
      361 gctcgctgca gtgcatgctt caatatgcga ttagatcttg ttgcgcaaaa agcgcaagaa
      421 ctaggatacg attactttgg aagtgccctg accatctcgc caaagaaaaa tgcagctttg
      481 atcaatcaga tcggaatgga tatccaaaag atttacggga cacaatactt gccaagtgat
      541 ttcaagaaaa acaaaggcta tgaacgctct ttgcaaatgt gcaaagagta tgatatttat
      601 cgccaatgtt attgtggttg cgtgttcgct gccaagcagc aaggatgcga tctgaaagaa
      661 atcaatcggg aagcaaaagc ttacttaaaa acatcaaccg tagtttttga ggaaaattaa
      721 agaaatcttt cggaaaaacg taagaaaaaa ataaagaatt tatggtagaa ataaggtaga
      781 ctgtataatt tgtccgtagt aaagtaagga aacaagaata aatttcaatg aaggaagaga
      841 taagatgcga aaagtagcac ctattttgaa gccaatcgct gtattaacaa tctcgacgat
      901 tgtcgcgact actacaggta ttgtgaatag ttccagctac cgtgcaccaa gttcaagttc
      961 tagtactgta gaagaaacaa caccaactac ttcagtctcg acatctgaac caacaacaac
     1021 ggaaagtttg aatgaaacca ctacgacaag tacatgggac gatacaacaa ctagttcgtc
     1081 gtatgatgag acatatacaa ctgacagctc aagcgaaaca agtaatacgg taccagatgg
     1141 cactccatcg gattcaacca atccaagtac aagtccggat acaagtgcta gctccgaaac
     1201 gataccaagc agtacgactg tagatgataa cacgcaaaat gatgatgcag cacgtacagc
     1261 acagcaaaac caagattcag gaaataccca aacgtataac cagcaagcag gtgattctca
     1321 atgacatttt tactagacct tatcaacggg gtgcataaat tccttggcta tttagacatc
     1381 agtcctaaat atcttaaccg tggatatacg attttaagtg tgattcccac gctttattta
     1441 ttgagaattg tttatggact ttggcaaaac caaaactatc ttcaattttt cttatatgga
     1501 cttgcatttc tagtgcttgt ttattttact gttttaaatg tattttatta ctttttagat
     1561 aaaaacacga aagccgatgt cactcagctt tttgtgaagt atttacctga tgaagcgttc
     1621 aacatccaga cagaagaatc gatagataat ggaaattcga tcgataaagt gaatacacaa
     1681 gaggtcatgg tttcttatga cgaagattat caattgaaat tagccgagaa tatgcggtat
     1741 ttgattggaa atggtgagat caaaacaaat gaactcggtc gcatggatgg atttctggtc
     1801 gatagaaata cattgtatcc ttattactat gtcaagaaaa caggagagaa agagtatgtt
     1861 ctaaatattg gacgaagcta ctcggacatg gaaaaagtag ggaccattcg acttgactca
     1921 acagaagaca cattggaacc tgtcggattg ttcatcgtcg gcggtgattt tgtcaaagac
     1981 gggttacgct atcatgagcc ttatcgtctg aaattgattg caaaaaaggc acagtcgcag
     2041 gaagaaccag tggtttatag tcgtagccaa agaagaagac attcataatg tgatgaaacg
     2101 ccgtgtatca gtggttgcga taaaaacgtt tattcggaat aagggggatg tgacacgtct
     2161 tttgtcacgt tcctttttta tataacgaga aagataagac gaacacatca cttattcttt
     2221 cctttcattt ataaacattg tagaataaag aaagtacaac gaagtattgt gaaaaaacgt
     2281 aataaataac gaggaaaata caagtaggag gtaagctgtg aaagaatttt tgaaaaattg
     2341 ggggattttg atccttgtat tagcggctat tttattagct agagtgtacg tgtttacacc
     2401 agttactgta aacgggcatt cgatggaccc tacattaagt gatggacaac gattgatttc
     2461 atctaagatc tcaaactatg aacgaatgga tattattact acaaaggaac cgggggatga
     2521 agaacggatg attgttaaac ggatcatcgg tatgcctgga gatacagtca agatggaaaa
     2581 tgaccagttg accattaatg gcaaaaagta tgatgaacct tatctggatg agttcaaaaa
     2641 agaattttca gaagataagt tacaaggaga atatgcttat agctcaggat tccaagcaca
     2701 agcagaaagt tcatccacct ttacgagtga ctttgaatat actgtgccaa aaggaaagta
     2761 tcttgttcta ggcgataacc gcttgatttc aaaagacagc cgaatgtttg ggttagtcga
     2821 caaagatatg atccaaggaa aagttgtttt ccgttattgg cctctttcag aaattaaaat
     2881 catgtgatga aaaaataaaa agaagctgtg acatgttttt tgtcacagct tctttttccg
     2941 aaaattcctt cttatttctt ggagttgctc actttttttg caatctcatt ttaagctttt
     3001 tggctgatgg ataagaaaag cgaagcgttt ttgttcataa atagcttttc cccacgatgg
     3061 aaggtatgac tagtagcaga gacaaagccc gttttttcca atcgattatt caattcttct
     3121 tggctaaagc cattatggac tttcggatga ctaatttgtt cattcttgtc aaagtctacg
     3181 atgatcaatt gaccgccagg agctaaaatt tcataaagtt ttgtaagaat attctccgta
     3241 tcaggaatgt gtaaaagtac aagtgataga aggatgagat tagcttggac agcaggaaat
     3301 tcgacagaag catcagcgtg gatcgttcgg acatttttca gatctgctgc ttggatcttt
     3361 tcttctgcca ttttcagcat agtttctgaa gcatcggcaa tgatcagttc tttgaatctt
     3421 tccgcaagcg gtaaactaac aagcccagtt ccgcctccgt aatccagcag gactttttgg
     3481 tggctgtcgc gcggtagata agtagttagt tcttcgcgaa taatcttggc taatgcttga
     3541 cggtcagggg aatcataatg gttagcgatg gaatcaaaaa tattttttgt catcaaatag
     3601 ctccttacgt aaaactttga aattttaact tattatagca cgtgaaaaga aagaaagcat
     3661 gctatactca attcaaaaag ggagaatcta gaaagaatat gccgatatcg tttgtaaaag
     3721 atagagagga aaaaggaaag tgtgtccgag aaatattact agacttgcct gagtggtttg
     3781 ggcttcctga atcaacggaa aaatatatcg aggaatcaag taaactacct ttatggtgtg
     3841 aaaaaagaaa agaagaatat ttaggcttta tcactctttc acagacaagt gaagatactg
     3901 ccgaaatcta ttctattgta tgggaataaa aaaagactat catcaccaag gattaggaac
     3961 aaaacttttt aaagaagcag aaggttacgc agcaaaacat tacaaatatt tgcaagtgaa
     4021 gactgtagac gaggggcact actctatata tgatcaaact atttgtttct acgaatcatt
     4081 aggattttca aggttggaag tttttccaaa tttatgggat gaatggaacc catgtttagt
     4141 tcttgtcaaa aaattggaac aaaagtgagc aatagattta atactaatct gtgcttgatg
     4201 aaaattctca ctgtaaaaaa tgctgaaaat caagtataat accataggaa caaactggaa
     4261 ggtgaaaaag agcatggcac aactattttt taagtatggc gcaatgaata gcggcaagac
     4321 gatcgagatc ctaaaagtcg cacataatta tgaagaacaa aataagccag ttgtcttaat
     4381 gacaagcggt attgatacaa gagatggttt aggcgttgtt tcaagccgaa tcggactgaa
     4441 acgtaatgcg atgccgatat ttgaagaaac agacgtattt gacgtaattc agaaaatgga
     4501 aagcaaaccc tattgtgtgc tgatcgatga agcgcaattc ttaaaaaaga aacatgtcat
     4561 tgcttttacg agaattgtgg acgaattgga tattccggtt atggcatttg gtttgaaaaa
     4621 tgattttcga aacgagttat ttgaaggatc aaaatattta ttgctttatg cagacaaaat
     4681 cgaagaaatg aaaacaatct gttggttctg ccacaaaaaa gcaatcatga atttacatta
     4741 tattgatgga aaccctgttt atgaaggcga tcaagtgcaa attggcggaa atgaagctta
     4801 ttaccccgtt tgtcgaaaac attactttca cccatcaatg attacagaag gagaataagc
     4861 atgtacgatc aattacaagc aattgaagac cgttatgaag agcttggaga acttttaagt
     4921 gacccagaag tgatttctga tacaaagcgt tttatgcagc tttcaaaaga agaagcaaac
     4981 acgcgcgaaa cagtcgaagt ttatcgtcgt tataaagaag tagtacaagg aatcaccgat
     5041 acagaagaac tgcttggtga aaaactggat gatgaaatgg ctgaactagc aaaagaagaa
     5101 ttatctgaat taaaaaaaga aaaagaagtc ttagaagaaa aaatcaagat cttgctttta
     5161 ccaaaagatc caaatgacga taaaaatatc atcatggaaa tccgtggagc agcaggcgga
     5221 gacgaagcag cattattcgc tggcgatctt ttcaatatgt accaaaagta tgctgaagca
     5281 caaggctgga aaacagaagt gatggaagct aacgttaccg gaatcggtgg atataaagaa
     5341 gtgatcatga tgatcaccgg agacaacgta tattcgaaat taaaatatga aagtggtgcg
     5401 caccgcgtcc agcgtgttcc ttcaactgaa tcgcaagggc gtatccatac ttccaccgca
     5461 acagttgtcg tgatgccaga agctgaagaa gtagagatcg atattgaaga caaagatatc
     5521 cgtacagata tctatcatgc ttcaggtgct ggcggacagc acgtcaataa aacagcatct
     5581 gctgtacgtt tgactcactt accaacaggg atcgttgttg ctatgcaaga tgaacgttct
     5641 caaatcaaga accgtgaaaa agcaatgaaa atcttgcgtg ctcgtgtgta cgataagatc
     5701 cagcaagaag cacaaagcga gtatgatgcc aatcgaaaat cagcagtcgg aactggggat
     5761 cgttcagaac ggatccggac gtataatttc ccgcaaaatc gtgtgactga tcatcgtatc
     5821 ggattaacta tccaaaaatt agatcagatt ttagcaggga aattagatga gatcgttgat
     5881 gcattgatca tgtatgatca aacatcaaaa ctggaagcta tgcaaaatgg gtaaaatgac
     5941 ctatcgagaa gtcctgtcac gggcttcttc ttttttagag gatcatggga aagaagcata
     6001 tagtatccaa tttttatttt tggaacgaaa acaatggaaa aaacttgatt ggctccttca
     6061 tatgaacgaa gagatttcag aagaagaaca gcggttgatc gaaaccgatc tgcgacttct
     6121 gcttgcagat catccacctc agtatttgct aggatatgca gatttttatg atcatcggtt
     6181 aaaggtaact gaagcaacac tgatccctcg accagaaaca gaggagttag tcgaatggtg
     6241 tttagacgag acgcctggtg taccgttaga agtgatagat attgggacag ggactggagc
     6301 aattgctatt agtttgaaag cagcaagaaa aaattggcat atatctgctg tggatctttc
     6361 agaagaagca ttagaagtag cgaaagaaaa tgctcagaaa gaaggaacga aaatcagttt
     6421 ttatcatgga gatacattag gaccagtaat ggatcagcaa tttgatgtga ttatttctaa
     6481 tccgccgtac atcagtcgga atgaatggga actgatggat gaaagtgtcc ggtcattcga
     6541 acctaaaatg gccctttttg ctgagaacga tggattagct atttatgaaa aaatagcagt
     6601 tgaagcacca agcgtactaa aatcaaatgg aaagattttt ttagaaatcg gatttcgcca
     6661 aggtgaagca gtaaaaaaaa tatttcagca agcatttcca gataaaaaag tggcgataaa
     6721 aaaggatctt tttggcaatg aacgaatgat ccaagtttat ggttgaagtt gtggatgaaa
     6781 aattttttat ttgttttttt attataaaaa aacacttatt tgacaaggat gtaaacgact
     6841 ttttggttta tcaacaagtt tatccactat tgacaaatag attatccaca aacagtggaa
     6901 aacttgagga taaactcaga caaaataaga atgtatgaaa aaggggttgt ggataaactg
     6961 tggaaacgaa acgatatgat gaaacagaat tgcaagaggc agccgaagca ctgaaagaag
     7021 gagaacttgt tgcttttcct accgaaacag tatacggatt gggcgccaac gctcttttac
     7081 caaacgctgt caagaaagtg ttttctgtaa aagggagacc gcaagataat ccattaatcg
     7141 tccatgtcgc ctcttttgaa caagtaaaag agtatgtgga caactttcat ccggcagcag
     7201 agaagattgt gaagaacttc tggcctggac cattaacttt gatatttaag atcaaaaaag
     7261 acactttgcc ctcagttgtg acgggaggat tatccacagc tgcatttcgt ataccggata
     7321 acaaaaaaac actagaagta atcgaacttt ccggtgttcc gcttgtcggt ccaagtgcaa
     7381 atacgtcggg aaaaccaagt ccaacgactg cagatcatgt atatcatgat ctacaaggaa
     7441 aaattaccgg gattattgat gatggagcta cgcgaattgg tgtagaatcc actgttcttg
     7501 atttaagtga tccaacagcc atgcctatga ttttacgacc aggtgcagta acgaaagaac
     7561 aaatcgaagc agtcatcgaa tctcctgttg ctattgatca gcatcttgta aaagaaaatg
     7621 aaacaccaaa agcccctggg atgaaataca aacactactc acccgataca cgtgttctga
     7681 tggtcagaga tggcgattgg tcaactgcag ttcaatgggc aaaaaataag aagatccgtg
     7741 caggtgtcat agcaagcccc gaaattgcgg atcaagtgcg gacagatact gcagctgttt
     7801 atatgtataa cgacaactca gtagaagcgg cggctaaagg attgtttgca ggtctaagag
     7861 gattagatga accaacactt ggattagatt tgatttttgt acaagtgtat ccagaaactg
     7921 gattggggaa tgcttacatg aatcgcttga aaaaagcagc aggacaaaat tattttgaaa
     7981 aatgatcaat tggttattgt cgcattcttt tagtatgata caatgctatc aagtaaatct
     8041 tctatcagtg aacgaaaaac taagcgtaac taatcgtaat agaagagata cgaatatttg
     8101 gcaatttgtt tttgatggga aaaataaaat tgtaggaaaa taaaaaggag ctgttgtttg
     8161 tggtagatta caaaacgttt gaccctgatc tttgggctgc gatcgccaaa gaagaagaaa
     8221 gacaagaaca taatttggag ctgattgcct ctgaaaactt tgtctccgaa gcagtgatgg
     8281 ctgcacaagg aagtatcttg acaaataaat acgcagaagg ttatccaggg catcgctatt
     8341 atggtgggtg tgaatttgtc gatatcgttg aaaatttagc gattgaccgt gcaaaagaat
     8401 tattcggagc gaaatttgcg aacgtccagc ctcactccgg ctctcaagcc aacacggctg
     8461 cttatctcgc tttggtagaa ccaggggaca cgatcttagg aatggatctt tccgcaggcg
     8521 gacatctaac acatggatca ccagtcaact tcagtggcaa aacctatcac tttgttgctt
     8581 acggtgttga tccgacaaca gaagttattg attataatgt ggtacgtatt ttagctagaa
     8641 aacatcaacc aaaattgatc gtagcaggtg ccagtgctta cggacgaacg atcgactttg
     8701 cgaaattcag agaaatcgca gatgaagtag gcgcaaaact gatggtagat atggcacata
     8761 tcgcaggatt agtagcagca ggacttcatc ctaatccagt tccttatgca gatatcacaa
     8821 cgactaccac tcataaaaca ttgcgtggcc ctcgaggcgg catgattttg acaaacgatg
     8881 aagcattagc aaaaaaaata aatagtgcgg tatttcctgg tattcaaggc ggccctctag
     8941 agcatgtgat tgcgggtaaa gcagtagcat tcaaagaagc attagatcct gcatttaaag
     9001 aatatagtga acaaatcatc gccaatgcaa aagcaatggt caaagttttc aatcaagcaa
     9061 ttggaaccag agtcatttca ggggcgacag ataatcatct gatgttgatc gatgttcgcg
     9121 agttaggtat aaatggaaaa gaagcagaga gcattttgga cagtgtgaat atcactgtaa
     9181 ataaaaactc gattcctttt gaaacactaa gtccattcaa aacaagcggt attcgaatcg
     9241 gcacgccagc aatcactact cgcgggttca aagaagaaga tgctgtaaaa gtggctgaac
     9301 tagttgtcaa agcattacaa gcaaaagatg ataacgctca actagacgaa gtcaaaacag
     9361 gtgtacgcga attaactgaa aaatttccgt tgcataaaaa ataaaaattt aaattccatt
     9421 agtaaacaga aaagttcaat gttgggcttt tctgtttatt ttttgggaaa acggattctt
     9481 tgacaatatc ggtgatgctc tctagtatat gaggtgtaat tttagtcttc acactaaatc
     9541 tcagggagga aaagtcatga ctgatcgtat ctcgttagaa aaggaagcag tagcattcag
     9601 tgatgcaaat gcgcctcatc cgcgtatcta tgaactagca ccggaagaag gaagagtttt
     9661 actagaagaa gtacaagaat cacctgtgaa caaatattcc gtcgatattg aagacttgat
     9721 ggtggatact ggcgaatggg gaaaactcaa tattcgtctc gtacgaccag aagggaatat
     9781 ggataaatta ccagttattt tttatattca tggagcaggc tgggtttttg gcagtgctca
     9841 aacccatgat aaattgatca gagaacttgc ggttcgtacg aattccgtcg ttgtctttcc
     9901 agaatatagt cgatcaccag aagcaaaata tccaacagca attgaacaaa attatgctgt
     9961 gttgcaacag ctgaataaat tagccaagtc aaaaaatctg gatctcgaga atctaatcgt
    10021 tgcaggagat tcagtaggtg gtaacatggc tacagtcatg acgatcttaa caaaagaacg
    10081 aaagggcctc ccaatatcca agcaattgct gtattatcca gtcacaaacg ctgattttga
    10141 tacagattcc tatcatgaat tcggagaaaa ctattttttg acaagagaag gaatgatgtg
    10201 gttttgggac caatatacga cgaatgaaga agagcgtgct gaaatcacag cttcgccttt
    10261 gagagcaaat ataaatgaac taaatgatct tccttcagca atgattttaa cgggagaagc
    10321 ggatgtatta agagatgaag gtgaggctta tgcgcgcaaa cttcgtgatg caggggttcc
    10381 tgttacacaa gtacgcttcc aaggaatcat ccatgatttt gtcatggtaa atgctatgga
    10441 tcagacaaag gcaacgcgtg cagcaatggc attatctact tcttggatca agaaaaaaga
    10501 ataagctttg cttgtccgca ggaaaacttc ttttcaaaaa cggattttct taggaaaaat
    10561 ggcgagtttg actagtcaat cacttgattt tgttttacaa ttaaaaagaa acgacagaac
    10621 aaaggagagc ttaaaaaatg ggcaaatttc aagtgatcga tcatccattg attcaacaca
    10681 aactaactat tattcgggac aaaaactgcg gaacaaaggt ttttcgtgaa gtagtcgatg
    10741 aaatcgctat gttgatggca tatgaagtat cacgggatat gccattggaa gacatcgtga
    10801 tcgaaacacc gatcactgaa acaacacaaa aaacgttgtc tggtaaaaaa gtagcgatta
    10861 ttccaatctt gcgcgcaggt attggaatgg ttgacggtat cttggaattg attccagctg
    10921 ctaaagtggg acatatcggt ctttatcgag atgaagaaac attggaacca catgaatatt
    10981 tcgtcaaact tccagaagac attgatgcac gtcagttgtt tgtagtagat ccaatgttgg
    11041 caacaggcgg atcagcaatc atggcaatcg atgcattgaa agaacgtggc gcgtcgaaca
    11101 ttaaatttgt ttgtttggta gcagcacctg agggagtaaa agctttgcaa gaagcgcatc
    11161 ctgatattga catctataca gcttctctag atgaaaaatt gaatgaacat ggttatatcg
    11221 ttcctggact aggggatgcg ggtgaccgtt tgtttggtac aaaataagta aatacgaaaa
    11281 agggtgtgac acaacttttg tcacatcctt ttttggtcat ttttaacgtt tgatgcagaa
    11341 cagctttttc acagctttcg tttgcatata aaaaaaccat cccaaaaatg cagagatggt
    11401 caaattcaaa tcttttttag ttctttgtag ttaacgatta ctacaccctt actttgcgtc
    11461 atcaagttcg cattttctgc agtttcatca atctcaacaa cagcggaatt tttcaattgc
    11521 tttgaaatcg ttccagtttg cgacttgccg tgaattgtga acattacgtg agtattgatc
    11581 ttgaattttt caccaatttc cgatggtacg atagttgtat caaattttcc aaaagttgcc
    11641 attttcataa aacctgcctt tcacatcaat tataccacgt tttatgaaaa atatcatgaa
    11701 attaaactaa ttttccatcg gactctttac aatcagccaa atagagtata taatttcttt
    11761 tggataaggc cagcttagct cagttggtag agcaacgcac tcgtaacgcg taggtcgtag
    11821 gttcgatccc tatagctggc attaaaagag aagacagata tatcaaggat ttttaatcca
    11881 ttgatacatc tgtctttttg tttaaaggaa ataaagtatg ctttcttagc aatagagcat
    11941 gtattatttc tataaaaatt taggagaata aaacgactag caatatagct gtgaaatcaa
    12001 atatcacatg aactgcccaa cttgtaatga tcgagttgct tttaatggca gcttggttga
    12061 ataacaatct tgccacccct tgtatcaaaa ggatgtgtgc tacagtgtgt aaaatatttc
    12121 cattatcata agtactgtaa tggataaatc caaagatgat tgctgacaag aaactagcga
    12181 caggaatgct gaacttacta gaaaaaatag cgagaaaata cactgagaac aattcttctc
    12241 caagtatcat gataggcaaa ataataaata ataacgggct gaaagcctca tttattggat
    12301 tgcctgttaa accccatttc aaaatttgtt gcaaaaatac cgagactgcg gcactgatta
    12361 ctacattgat aaaaaaatat ttggctatat ttttgacagg agcgacaggt ttcgagaaca
    12421 tttttttcgt atctttttta ccaaaaacaa gtgtaaatgc tataaatcca gtgatagtta
    12481 aagaaaaaat gctaataatg gatggcgcaa atagc
//
LOCUS       DACDKL010000073        12512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDKL010000073 DACDKL010000000
VERSION     DACDKL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.073"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..567
                     /locus_tag="IV329_001543"
     CDS             <1..567
                     /locus_tag="IV329_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP8940720.1"
                     /translation="IRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPE
                     NYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITV
                     PRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNT
                     NIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            634..1128
                     /locus_tag="IV329_001544"
     CDS             634..1128
                     /locus_tag="IV329_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8940721.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1184..1822
                     /locus_tag="IV329_001545"
     CDS             1184..1822
                     /locus_tag="IV329_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940722.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1894..2289
                     /locus_tag="IV329_001546"
     CDS             1894..2289
                     /locus_tag="IV329_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8940723.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2273..4726
                     /locus_tag="IV329_001547"
     CDS             2273..4726
                     /locus_tag="IV329_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8940724.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4723..6750
                     /locus_tag="IV329_001548"
     CDS             4723..6750
                     /locus_tag="IV329_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940725.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6747..7769
                     /locus_tag="IV329_001549"
     CDS             6747..7769
                     /locus_tag="IV329_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8940726.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7786..8715
                     /locus_tag="IV329_001550"
     CDS             7786..8715
                     /locus_tag="IV329_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8940727.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8960..9076
                     /locus_tag="IV329_001551"
     CDS             8960..9076
                     /locus_tag="IV329_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8940728.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9092..9895
                     /locus_tag="IV329_001552"
     CDS             9092..9895
                     /locus_tag="IV329_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8940729.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10070..10366
                     /locus_tag="IV329_001553"
     CDS             10070..10366
                     /locus_tag="IV329_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940730.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10373..12325
                     /locus_tag="IV329_001554"
     CDS             10373..12325
                     /locus_tag="IV329_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8940731.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12397..>12512)
                     /locus_tag="IV329_001555"
     CDS             complement(12397..>12512)
                     /locus_tag="IV329_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8940732.1"
                     /translation="EVNYEEWNEVFAQKGIKNDKNPYNYYFHVYERKNLLS"
BASE COUNT         4317 a         2053 c         2467 g         3675 t
ORIGIN      
        1 ataagagttc cttttgatga tttagacttt tttgaaagtc ataaagattt gattacccag
       61 acagcaagct ccatatatga taaacaaggt gataatgtat tagtggatac tattttagta
      121 ccacttccag agaattacga agtaattaac tttagtcagt tgaaaattag tgatgtggtt
      181 tcacaagcta ttgaagacgc tgagtctttt atgagtaatg gtgaatatca acgtgccttt
      241 gacagagttc acactgcttt tcatggatat ttaattgaaa tactaaaaaa atatgaaatt
      301 acagttccta gagacgaaaa tctatctaaa ttatatagta ggatacaaca attaattgaa
      361 aaagaaattc aacctactga acttgctgat atagtgaaga caacgataag gtcatcaaat
      421 ggaatgatta gctctcttaa tgaagcaaga aataggcatt cacttgcaca ccctaataca
      481 aatattattg gaaagagaga agcaaaacta ataataggta tttcaagtac agttacagat
      541 tatattagcg gataccttga taagtagcgt cattaactga ttgctacttt ttttatgact
      601 atttttataa aaaggatgtg ataagaattg gaaatgaaag tctatgtggc aaatttagga
      661 cggtataatg aaggtgaatt agtcggagca tggtttactc caccaattga tgaggaagaa
      721 atggcagaaa gaatcggttt aaatgaggac tacgaagaat atgcaataca tgattttgag
      781 cttccttttg atgtggacga atacacgccg attagtgaaa tcaaccgtct atgtgaagcc
      841 attcaagaaa tcgaaggtac acctatttat aatgaactca aagaaattca aggcatgtgg
      901 tttagtagct tggaagaatt actggaaaac aaagaggata ttcattgtta tagtgattgt
      961 gattcgatgg aagatgtcgc ccgttattac gtggaagaaa caggacagct tggtgaagtc
     1021 ccttccaatt tacaaaatta tattgactac caatctttgg gacgtgatat ggaaatagag
     1081 ggtaattacc tagttacctc tcatggggta tttgaatatt gtcagtaagt ttaacaatcc
     1141 gtgttataat taaattaata aatagcacgg agaggtataa attatggtaa atgtatttga
     1201 aaatcttaaa ctattaaaac tagacatgaa agataaaggc tgggtgatag attcctttta
     1261 ctttcgttac aaacagcaaa attatattgt acttgttaag ctatttgaaa aagaggaaaa
     1321 agttccagaa tatgctctgt taaaactaga atttttaaag gaaaacgatt tttcagatat
     1381 gctagctgtc tatgctaatt cggtaaaatt atatacagac acgaaaacaa taagagaata
     1441 ttttggtatc gaatacagta gtaacttggg agatgtttta tttcaattta gccaaactct
     1501 tgctcgattt attccgacag aagttagtga gaagaaaaat gaagaccaaa aagaagccat
     1561 gtgtttctca ttaagtcaaa gtgattcaga agatcccaga aaaaaatatt gtttttcagt
     1621 tagaagaaat cctttaaagg gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa
     1681 taaaacgaga ttgtatagac ccagcttata tgaacgatta gggtcagata ccaatttaag
     1741 tttcagatat tcaatgaatc cagatgatga agaaactgat gaaggtatta ttgcaaaatg
     1801 gacaaaaaat aaaattgaat agttaaagca ttgaaagagc aatcacctat tttgattgtt
     1861 ctttttctat ttttacggaa aggacgtgaa gacatgaaaa aaataagaag ttacaccagt
     1921 atttggtcag tagaaaaagt gatttatgcg attaatgatt tacaactccc tttcccaatt
     1981 acctttacgc aaatgacatg gtttatcgtt tctttgtttg tcgtcatact ctttggcagt
     2041 ttaccaccac tttctctgat tgacggagca ctactcaaat acttagggat tcccattgtt
     2101 ctcactgggt tcatgtgcca aaagaccttt gacggaaaga aaccctatgg atttctaaag
     2161 tctatcatta cctatgctct acgccctaaa gtaacctacg ctggcaaaca aatacgatta
     2221 aaaaaagaaa caatgaatga atcgattacg attgtaagga gtgaacaact agatggcata
     2281 tccgattaaa tatatggaag ataatctcgt atttaatcat gatggcgaag tattcgctta
     2341 ttatgaatta attccctata attacagttt tttaagttcc gatgaaaaaa ttcaagttca
     2401 tgataatttc tgtcaattga ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact
     2461 agcaaccgaa tcaagtattc gagccacaca ggaacaatcg aaaaaagaaa tcactggacg
     2521 attaaaagag atagcttacg aaaaaataga tgaacagacc gaagcgttaa tttctatgat
     2581 tggtgacaat caagtagact atcgcttttt tattggtttt aaactctacc tcaatgaaca
     2641 ggaagtatcc gtaaaaaatg ttgggaaaga aattcaaaaa gcatttgcgg aattctttca
     2701 tggtgtgaat cacaaattaa tgggcgattt tgtttctatg cctatggcgg aaatcgaacg
     2761 cttttctaag atggaacagt tgttagaaag taaattatcc agacgctttc agattcgtaa
     2821 attggagaaa gatgattttg gttatattat tgaacatctt tacgggcaaa caggtgtccc
     2881 ttttgatgac tacgactacc atttaccgaa acgatacgaa gataaaaaag tgttggtaaa
     2941 gaaatacgat attctcaaac ccactcgctg tttaatcgaa gaaaatcaac gctatttacg
     3001 gattgaacag gaagatacca ctacttatgt ggcgtatttt acaattaata gtattgttgg
     3061 tgaattggat ttccctaata gtgaaatttt ttattaccag caacagcagt ttactttccc
     3121 cgttgatacc agtatgaatg ttgaaattgt cgccaataaa aaagccttat ctaccgttcg
     3181 caataaaaag aaagaactga aagatttaga tagccacgca tgggaaagcg acaacgaaac
     3241 cagtactaat gtggtggacg ctttagaatc tgtcgatgaa ttagaaagta atttagacca
     3301 aaccaaagaa tctatgtaca aactaagcta tgtggtacgg gtatcagcaa agaatttgga
     3361 cgagctgaaa caacgttgtg atgaagtaaa agatttttat gatgatctgt ctatcaaatt
     3421 agtacgacct tttggcgata tgctaggctt gcataatgaa tttattccct caagcaagcg
     3481 atatatcaat gactatattc aatatgtcac gtcggatttt ttggctggtc ttggtttcgg
     3541 tgctacacaa atgattggtg aaaattctgg tatttacgtc ggttacaatc tcgatacagg
     3601 cagaaatatt tatctccaac ctagccttgc aagtcaagga attaaaggct ctgtaacaaa
     3661 tgcattagcg tccgctttta ttggttcatt aggtggcgga aaatcattct ctaacaatat
     3721 gctggtctat tattcagtac tctttggcgg acaggcaacc attgttgacc caaaagctga
     3781 acgtggtgaa tggaaagaaa ccttacctga tattgcccat gaaatcaata ttgttaatct
     3841 cacgagtgag aacgacaata aagggttact tgacccttat gtgattttaa aactcccgaa
     3901 agattcagaa agccttgcca ttgatattct cacgttctta acaggtattt ccagccgaga
     3961 tagcgagaag tttcctgtcc tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg
     4021 agggttgtta ttggtgattg atgaattgcg aaaagaagat acgcccatca gcaatagtat
     4081 tgctgaccat atcgaaagct ttgtcgatta tgattttgcc catctgttat tcagtgatgg
     4141 cagtgtcacc caatccatta gcttggaaaa acaactcaat attattcaag tggcagattt
     4201 ggtcttaccc gattccaata gctcttttga agaatacact accatggaat tattaagtgt
     4261 ggcaatgctg attgtcatta gtacctttgc tttagacttt atccactctg accgttccat
     4321 ttttaaaatt gttgaccttg atgaggcttg gagtttttta caagtggcac aaggaaaagc
     4381 tctatctatg cgattggttc gagcaggacg tgccatgaat gcaggggtct atttcgttac
     4441 tcaaaacgcg gacgatttac tagatgaaaa gatgaagaat aatattggct tgaaatttgc
     4501 ctttcgttcc actgacctag tggaaatcaa aaagacctta gaattttttg gtgtggacaa
     4561 ggaagatgaa aacaatcaga aacgcttacg tgaattagaa aatggacaat gtttagtttc
     4621 agacatctat ggtcgtgtag gggttatgca gtttcacccg atttttgaag agttgcttca
     4681 tgcctttgat accagaccgc ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag
     4741 aaatttcttc actatagttg gataacgctc ctttgtgtgg gcgttatttt acttttatta
     4801 ggcacgctgg gcaatgcagt acaagcgaca ggcttagttg atgaaaccat tgatacaagc
     4861 aatgaatact ccaagtatgg tttgaatcaa tatcagcttg attattatgt ggacaatagc
     4921 tggggctggc ttccatggaa ttggagtgat ggcataggac aatcggtgat gtatggattg
     4981 tatgccatta ctaattttat ttggacaatt tctctttatc tatcgaacgc cacaggttat
     5041 ttggtgaaag aagcctatgc cttagatttc attagtcaaa catcccaagc aattggcgaa
     5101 aacatgcaga cgattgcagg cattacaccg aatggctttt ccagttcagg gttttatgtc
     5161 ggtttcctcc ttctttttat tctcattgtt ggaatctatg ttgcctatac aggattggtt
     5221 aagcgagaaa cgacgaaagt gatacgagct gttttaaact ttgtcgtggt gtttattcta
     5281 tcggcttcct ttattgcgta tgcacccgat tatattgcga aaatcaatga tttttccaaa
     5341 gatgtcagta ccgccagtct gaatattggg acaaaaattg ttcttcctaa cacggatagt
     5401 caagggaaag acagtgtgga catgatacgt gacagcttat tctctgttca agtaaagcaa
     5461 ccatggctat tgcttcaata tggctccact gatgtagaag cgttagggga agaacgtgtg
     5521 gaaagtttat tgtctaccag tccagataca aacaatggtg aagatagaga aaatgtagtg
     5581 attgaagaaa ttgaagatca tgacaatacc tatttaaccc taccgaagac gatttccaga
     5641 ttaggaacag tcttcttttt gtttgtattc aatattggta tctcgatttt tgttttcctt
     5701 ctaacaggaa tcatgatttt ttcgcaagtg ctatttatta ttttcgctat gtttttgcca
     5761 atcagtttcc tattaagtat gttgccgagt tttgatggca tgggcaggca tgccattacc
     5821 aagttattta atgtgattat gacaagggct ggtattacct tgattattac cgttgccttt
     5881 agtatttcca ctatgcttta ttcgctgtca gcaagttctc cctttttcat gattatgttt
     5941 ttacaaattg tgacctttgc agggatttat tttaaactgg gcgatttaat gagcctattc
     6001 actttacaaa gcaacgactc tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa
     6061 atgatgaatc gacaaaccag aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa
     6121 gcagggtcaa cgaagcaaaa gacaatgaca acaaagcaga atcaaaccaa taaagctaac
     6181 catactcgtc aaaatgaacg gactacacca aagaaagaaa cagcactttc caatgtgaaa
     6241 aagcaacaag gcggtatcgg gcaacgatta ggtgaaaaga cagcccatgt attggacacg
     6301 aaaaatcgtc tagtcaataa aagaaagcag gcaaaacaac agatacaggc gacaccaacc
     6361 catgtcaaat atgcccttca tcaaggaaaa gagaatgtca ctcataatgt aacagacttt
     6421 aaacaatcta tggcagatac caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag
     6481 caaaaacgaa agaacattgc tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga
     6541 cgaaaagaaa acccatcatc tcctgttcag aataaccagc agcatgttcg caagcgtcag
     6601 caaaccgtca cgcctgtttt gtcacaaaac actaaacgtg aaaatcatca gcgaccattg
     6661 aaaaaagagc aaaaacaaaa gacaccaccg aagcaattca atcagaggaa gctaaataaa
     6721 agaacggata agcgaggaca acaaacatga agctaaaaat aacagtgatt tgtagtgtgg
     6781 tcttctctct cttctttttt ctcttattat tcctagtcat tttctttgca gatgatacgg
     6841 acagtggcga aaacaacaag gattcttcta ttccacaagg cggtgtgacc gtttcaccag
     6901 aagtgttggc acaccgtccg ttgattgaaa aatatggtaa ggaatatggg attgaagact
     6961 atgtttctta tatcttagcc attatgcaag tggaatcggg cggtacagca gaagatgtta
     7021 tgcaaagttc cgaatctttg ggtttaccgc ccaacagttt gagtaccgaa gagtccatca
     7081 aacaaggggt taaatatttc agtgaattat taaccagtgc agaacaacaa ggagtggata
     7141 tagattctgt tatccaatct tataattatg gcggtggctt tttaaattat gtgaggagtc
     7201 atgggaaaaa atatacctat gaattagccg aacagttttc taaagagaag tcgggtggtc
     7261 aaaaagcaga ctatcccaat cctatagcca tacctgtgaa tggtggttgg cgatataact
     7321 atggaaatca gttttatgtt cagttggttt cacaatacct aaccgacact tccccaacag
     7381 aatttgatga tgaaaccgtg caagtcatta tggacgaagc cttgaaatac gaagggtttc
     7441 cctatgtctt tggaggggcg tcgcctacca cctcttttga ttgtagtggc ttaatacaat
     7501 gggtgtatga caaagctggc atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta
     7561 cgcaagaaat ctctatggaa gaagcccaag caggcgacct tatcttcttt cattcaacct
     7621 ataatgcagg cacatatgtg actcatgtgg cgatatattt agagggaaat cgcttttatc
     7681 atgcaggcga cccaatcggt tatggcgact taagcagtcg ttattggcaa gaccatctga
     7741 ttggggctag acgtgttatt cataactaag aaaggaatga atcaaatgat tataaaaatc
     7801 gagcgaaaag aaaagaagga gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa
     7861 gttccgatga ttaaagttgg cacgcataag aaattaacct ttgttttatg ggttttattg
     7921 attggtagtg tcggatttgg aatctataaa aattttactg ccattgacac tcatactgtc
     7981 cgtgaaacag aaatcattaa acagcagatt gtcgatacca accaagtaga aagttttgtg
     8041 aaatcctttg cgaaagacta cttttcgtgg caacaatccc aagaagccat tgataaacga
     8101 aatgaaaaat tgacccatta tttgacggaa gaattgcagg tattaaatga agaaatgatt
     8161 cgtaaggata ttcctactag ttctagtgtg aacgatatac aggtgtggca ggtttctcaa
     8221 gtgaatgaga acacctttga ggtactgttt tctgttgaac aagttattac cgaagacaaa
     8281 gacaaggaaa ctatatcgtc tagctttcat gtggttgtcc atatagatga atcagataat
     8341 atggttatta tcaaaaatcc gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc
     8401 aaacagctag aaagtgacca tacagtagat acagaaacga tggacgaaat cactagcttt
     8461 ttagaaacgt tcttccagct ctatcctacc gccacagaaa aagaattgac ttactatgta
     8521 agtaatcatg tgttacctat gattaacaag gaatatgttt tcgaggaatt ggtgaatcct
     8581 atctttacca gaaaagataa ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa
     8641 gaaacaaagg caacccaaat ctcgcaattt gagttgatat tagaaaaaca agataattgg
     8701 aagattgtaa aataacaaat attggtacat gattacagat actttgtaat catgtactct
     8761 ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagtg
     8821 actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
     8881 atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt tgtacccagt
     8941 ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta tgctttgtat
     9001 acctatggtt atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc
     9061 ccttttgggt ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
     9121 tcatgttgat gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
     9181 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
     9241 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
     9301 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
     9361 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
     9421 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
     9481 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
     9541 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
     9601 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
     9661 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
     9721 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
     9781 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
     9841 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
     9901 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
     9961 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
    10021 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
    10081 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
    10141 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
    10201 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
    10261 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
    10321 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
    10381 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
    10441 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
    10501 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
    10561 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
    10621 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
    10681 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
    10741 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
    10801 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
    10861 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
    10921 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
    10981 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
    11041 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
    11101 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
    11161 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
    11221 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
    11281 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
    11341 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
    11401 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
    11461 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
    11521 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
    11581 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
    11641 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
    11701 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
    11761 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
    11821 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
    11881 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
    11941 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
    12001 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
    12061 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
    12121 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
    12181 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
    12241 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
    12301 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
    12361 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
    12421 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
    12481 tacctcattc cattcctcat aattcacttc tg
//
LOCUS       DACDKL010000074        12330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDKL010000074 DACDKL010000000
VERSION     DACDKL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.074"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            61..1182
                     /locus_tag="IV329_001556"
     CDS             61..1182
                     /locus_tag="IV329_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8940733.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1440..2942
                     /locus_tag="IV329_001557"
     CDS             1440..2942
                     /locus_tag="IV329_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8940734.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3009..3536
                     /locus_tag="IV329_001558"
     CDS             3009..3536
                     /locus_tag="IV329_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8940735.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3747..4808
                     /locus_tag="IV329_001559"
     CDS             3747..4808
                     /locus_tag="IV329_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8940736.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4811..5707
                     /gene="murQ"
                     /locus_tag="IV329_001560"
     CDS             4811..5707
                     /gene="murQ"
                     /locus_tag="IV329_001560"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8940737.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5721..7703
                     /locus_tag="IV329_001561"
     CDS             5721..7703
                     /locus_tag="IV329_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8940738.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7768..8214)
                     /gene="nrdI"
                     /locus_tag="IV329_001562"
     CDS             complement(7768..8214)
                     /gene="nrdI"
                     /locus_tag="IV329_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8940739.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8425..9849
                     /locus_tag="IV329_001563"
     CDS             8425..9849
                     /locus_tag="IV329_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8940740.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(10030..10803)
                     /locus_tag="IV329_001564"
     CDS             complement(10030..10803)
                     /locus_tag="IV329_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8940741.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10800..11705)
                     /locus_tag="IV329_001565"
     CDS             complement(10800..11705)
                     /locus_tag="IV329_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8940742.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11859..12179)
                     /locus_tag="IV329_001566"
     CDS             complement(11859..12179)
                     /locus_tag="IV329_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8940743.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         3960 a         2188 c         2667 g         3515 t
ORIGIN      
        1 tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa gaagaaagtg agggatacag
       61 atggatattt tggcggcaat tgattcattt aaaggaagtg cttcttcatt tgaactaaat
      121 caggcagttc taaatggctg ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca
      181 gatggcggag aaaatactgc agaagcaatc tattacgcgc tcaggggaac atggcgtacc
      241 attgatggga tggatctctt attccgtcag cgaaaaatca gctatttatt gacaacttgg
      301 caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga tctacttgat
      361 aaaccaacgg atcaaactat ccgacttgct tccagttatg ccttaggaga acttattaaa
      421 gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag cgggtgtagc
      481 gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga
      541 aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa
      601 gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct
      661 tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa
      721 gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca
      781 ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt
      841 tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat
      901 gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac
      961 ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga
     1021 gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt
     1081 ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta
     1141 ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct
     1201 ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa
     1261 gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag
     1321 attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt
     1381 gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa
     1441 tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat
     1501 ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg
     1561 gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag
     1621 taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa
     1681 cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc
     1741 aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag
     1801 agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg
     1861 atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa
     1921 agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg
     1981 accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct
     2041 atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa
     2101 aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag
     2161 aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata
     2221 aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc
     2281 ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt
     2341 ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc
     2401 aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc
     2461 gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg
     2521 gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt
     2581 atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc
     2641 aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt
     2701 ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat
     2761 ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct
     2821 tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg
     2881 aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct
     2941 aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag
     3001 agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag
     3061 tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag
     3121 cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac
     3181 gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg
     3241 aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact
     3301 ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg
     3361 tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc
     3421 tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta
     3481 atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat
     3541 gtttttgtgt tatttaaata aaatatttca aggcactgct tttgcttagc agtgcctttt
     3601 ttaggttatg acaattcttt tgataatata aactcaacct ttagaaatga aatttaatta
     3661 ttttaataaa agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa
     3721 gacggataca gaaaaagagg gataatatgt ttggcttttc aatatttatg aacgattctt
     3781 tgtcagaaga gaagaaacag tatatccgcc aaatggcatc caatggcttt gtcggtattt
     3841 tcacttccat gcatatacca gaagatgatg tttcggcata tcaaaagcga ttgatcgaac
     3901 taggaagcga agcgaaaaag aataaactag aattgatggt cgatatttca ggagaggcat
     3961 tggaacgagc gggtttttca ttagaagatc taacgccttt gaaagcaatt ggtgtaacag
     4021 gtttaagaat ggattatcat atttccaatc agcagattgc cgactggtca catcaattga
     4081 aaatcagttt gaatgctagc acgatcacgc ctaaagatat cgatgaattg aaagaagcag
     4141 aagctgattt ctcacagatt gaagcttggc ataattatta tccacgtccc gaaacagggc
     4201 tggacaaaga atggtatcag aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc
     4261 aaggttttgt accaggagat acagagttga gaggcccact atatcaagga ttgccaactt
     4321 tggaagaaca tcgagggatc catcctcttg caggcgctct agatctatta gcaagtaaca
     4381 cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt
     4441 ctttccaaaa agaacagaca gtcttgctcc atacggaacc tgtagatgaa gaattttacg
     4501 aatatatatt agggaagcat acgaatcggc aagacgatgc aagagatgtc atacgaagcg
     4561 tagatgcacg ttttcgagag attccgccta tcccagcaag aaatacagct acgcgtatga
     4621 aagggtctat tactttagat aacgaaaagt atttgcgcta tatgggtgag atccaattaa
     4681 ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag
     4741 agctaccatt gatcaaccaa atcaaagctg gtatgaacta tcaatttata agaaaagaag
     4801 gaagataaaa atgagcaaaa tcaatttaga gaacctaaca acggaacgca gaaaccaaga
     4861 aacttttgga ttagatgaga tgagtatttc tcaagccgtt caactaatga acaaagaaga
     4921 tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag tgattgctaa
     4981 cacgattgag tcctttaaaa aaggtggacg tctgatttat atgggagccg gaacaagtgg
     5041 aagactaggt gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat
     5101 ggtcataggt ctaattgctg gtggagaaga agcaatgact gtagctgtcg aaggggccga
     5161 agattcttat gagctagggc aagaagatct aatcaagctg aatttgacaa aaaatgatat
     5221 agttattggt atcgcagcta gcggtcgtac accatatgtg atcggtggat tagattacgc
     5281 aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat aaacaagccg aaatcagtaa
     5341 acacgcggat ttcccaatcg aagtcgatgc aggtccagaa tttttgacag gctctactcg
     5401 attgaagtca ggaacagcac aaaaacttat cttgaatatg atttctacta tcagtatgat
     5461 tggtataggt aaagtattca ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact
     5521 aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa gctgattacg aaacagctga
     5581 aaagtacttt gaagaagcag atcaaaatgt taagctggct atcgtcatga ttttgacaaa
     5641 tagtgataaa gaggaagctc aagcaaaatt agctgaagca aacggatttg taaaagggac
     5701 cttatgaggg ggaaaagacc atggcaaaaa acgaactgac tttggaagaa ctggcaaaac
     5761 aaatttataa aggggcaggc ggcatgggca atgtagaaag cattgtccac tgcatgactc
     5821 gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc
     5881 caggagtgct tggtgtagta gaagatgaac agcttcaagt catcatcgga cctgggaaag
     5941 tgaacaaagt agcacagcaa atggttgatt tggcaggcgt gaaattagga gaaaaacttt
     6001 cttctgtaag tacagctaca gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac
     6061 gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg
     6121 tattaaaaga catttctaat atctttgtgc caatgattcc tgcatttgtc ggtactggga
     6181 tcgtggcagg gattgccgca gttttagcaa acttagtcac agccggaaca cttgatgcag
     6241 caacatggca acaatatatc gatgtgatga acatcttgaa aaacgggatg tttagctacc
     6301 tagttatcta tacaggtatc aatgctgcac aagtttttgg tgcaacacca actcttggtg
     6361 gagttatcgg ggccgttgtg atgctgacag ggatgaatcc ggaagcacca ctgaaaaact
     6421 tatttactgg tgaagctctt gcagctggac aaggtgggat cctaggtgtc atcttcgctg
     6481 tttggctatt gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat gcggtagata
     6541 tcatcgttac accaactctt tcattgattg ccatcggtct gattgaaatt ttcttcatca
     6601 tgcctttagc aggtttcatt tctgatggca tggtcggtgg gatcaactgg gtacttggcg
     6661 ttggtggagc atttgccggc ttcgttctag gcacattatt cttgccgatg gttatgttcg
     6721 gactgcatca aattttgact ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc
     6781 tattgcttcc aattcttgcg atggctggtg caggacaagt gggtgcagca ttggcattat
     6841 ggattcgctg caaaaaagat aaagaattaa cagaaatgat caaaggagct ttaccagtcg
     6901 ggatcctagg aatcggcgaa ccattgattt acggagtaac attacctcta ggacgcccat
     6961 ttattactgc ttgtatcggt ggaggtatcg gcggggcagt gatcggaatg atcggaaatg
     7021 tcggggctat cgctattgga ccttcaggag ccgctttgat cccattgatc tctgacggta
     7081 aatggttagg ctacatcctt ggattattgg cagcttatgc tggtggcttc gttgctacat
     7141 tcttcttcgg tattcctaaa gaccgtttag aaaaagaaga attagcgact gaaacagcca
     7201 cagtaaatga agtgcctgaa aaagtagtac ctcaaacttc agctgaaagt gtgactatcc
     7261 tctcatcagt tgctgacgga acagttgttt ctttagataa agcaagtgat ccagtttttg
     7321 cacaaaagat gatgggtgaa ggctactttg tagaaccaga aaacggacat atctattctc
     7381 cagttactgg ggtagtcagc tctgtcttcc caacgaaaca cgctattggg atcacaacgc
     7441 caaatggttt agaaatcttg cttcatatgg gaatcaacac agttgactta ggcggtacgc
     7501 catttgatgt gaaagtaaca gaagggcagc aagtgacacc tgatacgctt gtagcagaag
     7561 ctgacttaga agcgatcaaa gcagcaggca aagaaacatc catgatcgtc ttggtaacca
     7621 atatggatcg tgtaaaaaac tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac
     7681 ctgtgatgga tgtcaaagct taatatgata gaaacaatag aaacgaaaga agaaactgaa
     7741 atctattttt cagcttcttc ttttttatta tttattgttt tctgctaaaa tctgataaat
     7801 tcgtgtcaca tcagatggtg ttccccgtaa ttcataatct gccaagaaag gaaagccaaa
     7861 tgtttgtgca tattgtttgg ctgttagaca atactgatta ttgaagtttt tatttccgct
     7921 accgactaca ccaagacaaa gtttatgatt gtcttcatgt tccagatatt cacgcatcgt
     7981 ttcggtcaag atttctgtat caccgttatc taaaccattt ccgccgtcta aataagtagg
     8041 aacgaaagta aaaaatggtt ctgtttcttt cgtaaaatca ctattttcat ggatttcttt
     8101 taaagtaact tcaggattga caggatcttc tgcatgcatt ttttcagcat attctgctag
     8161 atgtttggca aaggcacgcg tatttcctga aatcgatata tataaaatgt tcatggatca
     8221 tcctccaatt ctttcctttt agtatagcat cttttttaag ttttttggtg gtgtaatttg
     8281 gaaagggact aggcgctttg cttgtataat gagaaaaaag ccggtagaat tatatttcta
     8341 ttaaagtttt tattaattaa gtaaaaataa aaaaggtttg ttatactttt tgataaaagc
     8401 ggttttattt tggaggatgc ttacatggat acagagcaac agaaaaaagg aattggttta
     8461 attccacttg ctgccttagt aattggttca gcgattggag gtggcgtatt tggtattatg
     8521 acggacatct ccggatcggc tggcggccca gctattttca gttgggtact cgtaggaaca
     8581 gcaatgttga tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa
     8641 ggcgggatct ttagttacgc agaagaaggt tttggccgtt tcgctggttt tatcagcggt
     8701 tggggttact ggctcacatg ctggttaggc aacgtagctt ttgcaacatt gttgatgagc
     8761 gcattaggat atttcttccc agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg
     8821 agtacagtct ttttatgggg atatgcatgg ctagtgaatc ggggagtcga aaatgctagt
     8881 ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat tatttttatt tattgtagta
     8941 gctatcagca cgttttcgat ccaacaattc attgataact tttctagcga cattatattg
     9001 aatgcgaatg gacagccgat cccgttttcc agtcaagtat tgagttgcat catggtgatg
     9061 ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa
     9121 tcagatgttg ggaaagcaac gattctaggg atattcggct tgattattat ttacgtcttg
     9181 gtttccatgc ttccatatgg gacaatgacg atgaatcagc tgcaaacgat caaggcgcag
     9241 cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac
     9301 ggcggtttga tcatttcttt agttggtgtt tggctctcat ggacgatctt accgattgaa
     9361 acaatgggaa acatggctga agacggactt ttgcctaaag catggggaaa actcaacaaa
     9421 aatggggcac caacttatgc tatcgtattg acgacgttat gtacgaatgt ctttttactt
     9481 tcattattat tcactgacag tgcttataac tttgcttaca cacttggtac agcagctatt
     9541 ttcttcacgt ggcttttctt aggattgtac caaatgaaac tttcttatca gcggaaagaa
     9601 tgggtccaat ttttagtagg gcttttagca agtgcctttc aagtatgggc tatgctagtc
     9661 gtagccttta atgaagtaat gcttgttctt gttcttttct tgccaggcat ttatttttac
     9721 ctcaaagcac gtaaggagca aaacgagcct gtacaacata tccacttaga taagatttta
     9781 cttataggtg tgacgatggt cggagtgatt gcaatggttt tatttgccac tgggatgctt
     9841 cacgtataga agtataaaag aagtataaag agaggttgtg agcctgtcgt ttattcaaaa
     9901 acatactagg aaaggagctg tgacacaaat gttgtcacag ctcctttttc agaataaacg
     9961 gtgtttaaga gacagccaat cggcaataag gctgaacgtc tctttcacag cctctttttt
    10021 gatgagtcat catacatttt ttcgcttaaa tacgagatat cctaatgcta agaacaaaac
    10081 gatgtaagct aaattaccga atagcatttg attagtagaa agatgagtca attgcttcat
    10141 gatctcttcg tttccaattt ggttttgcag attcaacata ctgatcggat tccatttgat
    10201 ccactcccat ttttggatga gtgcaataag gataccagat atgattgacg aagcaaaata
    10261 aaagacgatt cctgctgaga tggatgcgcc ggaactattg ataaagcaag caatcatcaa
    10321 cactaaactt aaaatcagcc ataaaccgat ataactaccc aaagtataag tgaacaagct
    10381 ttggatcagt gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat
    10441 cagtttcatc aacacagtga caataatagc taaaaggtat aaataaacag aataaataac
    10501 taatgtgatc catttgctta ctaatatttc tcctcgagaa tactttcgat aaaggagatt
    10561 tttcaatgta ccatattgtg cttccatcga aatgatcgtg cttgcagctg caatcatgat
    10621 aaagacaatc caagatgtgg cactaaaaag ctgagcagtc atttcaactg gatcaatgat
    10681 cgtcgtgtat tttttggcta gaagtgctgt tcctatgagc aaaacaacta atagaacaga
    10741 taaaacagca gttgactttt tcttgatcaa tttaaataat tcttgtttta caagcaatgt
    10801 catttttctt cttccccctt tactgaatcc agaatttcca atagagaaga ttccaaatca
    10861 ttcgtcagat gctggacgtc tgtcacatgg atattttgtg cagacaatag ctggatcacg
    10921 gttcctaata attcttcgtt ttcttttaaa agcttgattt tatcaggatc ttccaaagta
    10981 tatccagctt ctgtcaagat cgttcgagca gctagatcat ttgttgtaga gataacgatg
    11041 aaatcttgcg ctgtttggtt caattcagcc attgtacatt gtttgatgat ctttccttga
    11101 ttgatcagga tcagctcatc gaccattttt tcaagctcgc tgagtaagtg actggagatc
    11161 aagaaagagg tgccctgagt agccaagtcg gtaatgattt ttcgcacatc tcttgttgct
    11221 tgtggatcca aaccgttcat cggctcatct aaaataacga tagacggatg gttcagtaag
    11281 gcaagagcaa ttcccaattt ttgtttcatt cccaacgagt aggatttcgc ttttttgtga
    11341 atgtactttt ccatcttgag ctgattcacg atagtgtcga tagcagatac atcgttggtt
    11401 tcagagaata acttcagatg atcatagcct gttaagaatg gatagatacc cgggtattcg
    11461 atcaatgctc ctacttgttc caacccttta tgactagagg gagaaatctc attttcacca
    11521 atacagattg ttccatctga gtaatggatc agtcctaaaa tagctttcat gatcgttgtt
    11581 tttccagcgc cattaggacc tactaatcca acgattttgc caggggataa gctaaaactg
    11641 acatcgctga gggcttgttt gtgaccaaag ttcttggata attcttggac cgaaagactt
    11701 ttcaaaaata ttcactcctt ttctatagat agtacaagca tacacctgat gagtggagaa
    11761 gtcgctaggt cattggactt atttcttatt ttttcataaa aaaattgaat attaaataaa
    11821 aaggcttatt ttttctgatc agtacttatt ccaagtgtct agtggccgct agtaatcttg
    11881 atgccaatga tactgatgag tagcatcccc acaaagaacc aagtggaaag attcagctta
    11941 tcatgaaaca agaccacacc aattatgacc gaaccaactg cgccaatccc tgtccagata
    12001 ggataagaga gactcatcgg caaacttttg attgcctttg acaaaaagta aaaactagca
    12061 atcatgccaa tcactgtgta aaaagaataa ttgattttag aaaaaccatc actccatttc
    12121 atcatcgttg accagaatac ttccaaaatt cctgctatga ttaattccat ccatgccata
    12181 cttacattcc tccttaacta taaaaaaata cacaaaaaga ggaaaatcat cccttcaaag
    12241 acctaatgga atgatcatcc tctttatcct acccggtggc agattgtata tctttttctg
    12301 tagtatagca cacctatcca gatatcggca
//
LOCUS       DACDKL010000075        12250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDKL010000075 DACDKL010000000
VERSION     DACDKL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.075"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(172..1341)
                     /locus_tag="IV329_001567"
     CDS             complement(172..1341)
                     /locus_tag="IV329_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8940744.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1545..2720
                     /gene="mltG"
                     /locus_tag="IV329_001568"
     CDS             1545..2720
                     /gene="mltG"
                     /locus_tag="IV329_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8940745.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2856..3335
                     /gene="greA"
                     /locus_tag="IV329_001569"
     CDS             2856..3335
                     /gene="greA"
                     /locus_tag="IV329_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8940746.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3504..4235
                     /locus_tag="IV329_001570"
     CDS             3504..4235
                     /locus_tag="IV329_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8940747.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4232..5299
                     /locus_tag="IV329_001571"
     CDS             4232..5299
                     /locus_tag="IV329_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8940748.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5306..5938
                     /locus_tag="IV329_001572"
     CDS             5306..5938
                     /locus_tag="IV329_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940749.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6078..6743
                     /locus_tag="IV329_001573"
     CDS             6078..6743
                     /locus_tag="IV329_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8940750.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6771..7064
                     /locus_tag="IV329_001574"
     CDS             6771..7064
                     /locus_tag="IV329_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8940751.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7132..8079)
                     /locus_tag="IV329_001575"
     CDS             complement(7132..8079)
                     /locus_tag="IV329_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8940752.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8257..9060)
                     /locus_tag="IV329_001576"
     CDS             complement(8257..9060)
                     /locus_tag="IV329_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8940753.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9206..10120
                     /locus_tag="IV329_001577"
     CDS             9206..10120
                     /locus_tag="IV329_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8940754.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10168..10638
                     /locus_tag="IV329_001578"
     CDS             10168..10638
                     /locus_tag="IV329_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8940755.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10723..11331
                     /locus_tag="IV329_001579"
     CDS             10723..11331
                     /locus_tag="IV329_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8940756.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11465..11665
                     /locus_tag="IV329_001580"
     CDS             11465..11665
                     /locus_tag="IV329_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8940757.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4116 a         1885 c         2471 g         3778 t
ORIGIN      
        1 ttggacagaa cagaaagaag aacacctctt tctgttctgt ttatttggaa ttaaagtctg
       61 tagcaaaaat tgatttttag atttgtccca cactctttcc tacgtttagt cgagtacttt
      121 ttttaagcaa atgctattca aagaagtagt atttgctatc ttttcatcat atcagctatt
      181 tgccaactta gtagttcctt ccatcaggtc atagatttgg tttcttagat cagcagtagg
      241 cagtttgata gttttgtcag cttttatatt gctttccatg atgatagcat cctgtgaatc
      301 tttgctaaat atagcagatg tatcaaaaga gtcgatattt ccttgcgaca ggatataatt
      361 atctccgcta tataccccac cactatatgg atgtccattc gcattttcac gccataatga
      421 gaagagtaag tctttggaaa ctagttttcc agataaaact tgtgtaaaca gcgtatttaa
      481 atcatagacg gacatactga tattaccagt ccctaattcg ttatggatct ctgtttcaga
      541 ttctgtcagt ggtttaaagt aatcatcctc tttagacatt tgataggata gtgcatgatt
      601 ggatgaattt ttgacttggt cataaaattt tgtatgctgt aatcctaaag gttcgatgat
      661 atcatcggtc aataattttt catacggttg atttgttaat ttgatcaaaa ttccagtcaa
      721 tatggtaaag ttcaatgaat cataatgaga ggtctgataa tctgtaaatt ttaagtgctg
      781 aatcgcaaat tgaacgactt cttcatcagt catcggtatg gttggagaag cagttcggtg
      841 caagcctgag cgcatataca gcaagtcacg gatagtaatt tgctggctgc cagcaatttg
      901 tggatagaat tgagccagct tagtatctaa agaaaggtgc ccacgctcga cttgttttaa
      961 aatcaaaaga gtagtcaatg ctttttgaac cgaaccaatc tgataataaa gagaaggatc
     1021 atttggttgt tgcgtgagtt tattcgcata gccatagcct ttttccatga tgatgcgatt
     1081 atttttcttt acgtaaattg agccaatata atcatttttt cttaatagtt gatcgatttt
     1141 ttcaacgagc tgttgttcat tggtagtcag tatcaacttt ttttgagctg attgagcatg
     1201 ggcaactatc tgagagtgtt cattattgta aaattgccat ccaagaatag ttcctgtaga
     1261 gaacgcacta aggctaaaca acacgacaac gatcattaaa aatgaatatt ttcttttttt
     1321 agcatgtttt ccttttttca taatagttta tttagtctcc tttctttttt acagaaagaa
     1381 taaagctaaa atagcttgta tcttgataac atttatttta atcttatagt aatggattta
     1441 ctacttacat ttttgataga atatttaaat aaaatcattc acgccaattc aaatatcatg
     1501 tataatgaat aaagttacga acttgatagt aaggaggagt tgttttgtca ggaaacaaac
     1561 aacacccaag taaagataaa aaagaagcca gtaaggcagc tacagcagat ataatcgaca
     1621 agcggagaaa tctgcgtaaa aaagaagata agattgtgag gaagatcatt ttagtgattg
     1681 ctcttacatt attgattatt ggcggatttc ttggatttac cgtgtatcgt tatgtagata
     1741 gcgggttgaa gcctttagat aaatcagatg accaattagt ccaagtcgag atcccgagtg
     1801 gctcttctaa caagcagatt ggtgagattt tggagaagga caatattatt aaaagcggga
     1861 ttgttttcaa ttattataca aaatttaaaa atttaactgg ctttcaagct ggctattatc
     1921 agttagcgcc taatatgact cttgacgaga ttggcaaaca gttgcaagaa ggcggtactt
     1981 ctgagccaac aaaagttgca gatgggaaaa ttgccattcc agaagggtac gatatcgatc
     2041 agattgctga acgggtagcg aaggtaactg gtaaagacaa gaaagaattt ttagatttag
     2101 tgaatgatga gactttcttt aacgagttgc accaaaaatt cccagaactg ttggaaagtg
     2161 catctaaagc tgagaacaca aaataccgtt tggaaggtta cttattccca gccacatatg
     2221 attattacac gagtacttcg cttaaggatc tagtgattga aatggtgaac aagacgaata
     2281 cggtcatgca aaactattat tcagctatca agcagaaaaa tttgactgta caagaagttt
     2341 taactctagc ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt aaaaacattg
     2401 cacaagtctt ctttaaccga atcaaagcaa atatgccact tcagtcagat atctctgttt
     2461 tatacgcatt aggtgaacac aaagaattag tgacgtatga agatacagct gtagattcac
     2521 catataattt gtataccaat accggctatg gacctggacc ttttgacaat ccaagcgaag
     2581 aggcaatcaa ggcagtatta gaaccagcag aaaatgacta ttattatttc gtagcagata
     2641 cgagcactgg aaatgtttat ttcgctaaaa cttatgaaga acatatggaa ttagtggaaa
     2701 aatatgtaaa taattcttag aaaaacaggc tctagacatt gatcattttt agaaaaacat
     2761 ttacatttga agaaaagcgt ggtaatcttt tttggactat attcctcaaa agattacctt
     2821 ttgcggtatt ttatcgaaaa aaggagacgt tacacatggt agagaaagtt tatccaatga
     2881 ctctagaagg taaagaaaaa ttagaaaaag aattagaaga actaaaaaca gtcaaacgcg
     2941 gagaaatcat tgaacgaatc aaaattgccc gcggtttcgg agatctttcc gaaaactctg
     3001 aatatgaatc agcgaaagat gaacaagcat ttgttgaagg aagaatcaca acaattgaaa
     3061 atatgattcg ttttgctcaa atcatcgata acgacggtgt agatgcagat gaggtatcaa
     3121 ttggtaaaac agttacattc atcgaactac cagatggaga agaagaagag tacacgattg
     3181 tcggaagcgc tgaagcagat ccgttttctg gcaaaatttc caatgattct cctattgcgc
     3241 aagcattgat tggcaaacgt aaaaatgatg aagtaactat tgctactcca ggtggcgata
     3301 tgttagttaa aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc tagagagttc
     3361 atcttactga tgatacactc tagatctttt tctctttgtg tattttagat ttcaacaaac
     3421 gatcttacat ctaaagtcct atagccaaca gttaaaaaaa taggtatgat gaaatgagaa
     3481 tgtgtcagga gggagaaaga gcgatgaata gttcttggcg tttttttgtg gtagttgaag
     3541 cactactgct gttatttgct gtttggcaaa tcgtaaacaa tactggactt ttgcttttag
     3601 tgatattcgg tattttcaat atatatctag ctatgcgcaa acatcctcgt actaaatttc
     3661 aaaattttca attgatatta ggaagtttgg tcattttttt tagcttagtc aacagtcctg
     3721 ctttatggat gatggctgta tttgccgttt tatttattgg tctgaaaggt gtagagattt
     3781 cagggataga tttaaccaaa aatgcttttt ggcgaaagaa acagatcatg atgatccaaa
     3841 cagagcaatt aaaaacacat aataatgagc ggaaaagaca gcaattattc ggtaaccaac
     3901 gtattggaaa cgacgtctat gaatgggacg atatcaacat cgccgtcatt tctggtgata
     3961 ctatcataga tttgggcaat acgcttttgc caaaagatga taatattgtg attgtaagaa
     4021 aagggatagg cagaacacgg atcttagtac ctttgggcgt agccatccga ttagagcatg
     4081 cgacattagt aggaaatgtc ttgtttgaag aagagcaatt ttctttgaaa aatgaacaaa
     4141 tcaaaatcta cagcagtgat tatgatgaaa accctcgtag attgaagatt attactaata
     4201 ccttgattgg agatgtcgag gtgatacgaa tatgatggga aaaatatcca gagcgatgct
     4261 agctgtttat tcggggattg cggctttcct tattattcta ttttcacttt tcacttattt
     4321 ttatgccagc aatcaaagtc attggtgggg agaattactg cgtgcacgtt tattatatgt
     4381 tccgcttatc ttccatttgt tagccatttc cttaggtgta ggattaattg tctttctatt
     4441 attatcactt attcaaaaga caaaatacgg gaaaatcgaa gaaaagctgc gtgcactttc
     4501 ttctggcaat tatgaatcca aattgttgct tcttccgatc ccaagtgcat cggacgattt
     4561 atacatcaaa gatattgata aggaaatcac taagataaaa gaaaaaatga ttgaagtatc
     4621 tagtgaatta caaattgtaa cgagccgtcc ccaatatgta gatggacaaa ccaaagaaga
     4681 aattttagaa ttagaaagac atcgattagc ccgtgagctg catgattcag tttcgcaaca
     4741 attgtttgca gcaatgatga tgatgtcggc attgacggag caagcagaaa aaagcgagac
     4801 accagaaatg tttcgcaagc agttgaaaat ggtagcagag atcatcaacg cttcccagtc
     4861 tgagatgcgt gcgctgcttc ttcatctacg tccagtcaat ttagaagaga aaagtttaaa
     4921 gcaaggtatc gagcagttat tgaaggaatt gcaaaataaa atccagattt cgctgaaatg
     4981 ggatgtagaa gatgtaaaac tatctagttc cattgaggat cacttgttcc gaatcgttca
     5041 agaattgtta tcaaatacat taagacatgc taaagccaat gaattagagg tatatttgca
     5101 caaaatagac aataatcttt tgttacgtat tattgacgac ggaacagggt ttaatatgaa
     5161 tgaaacgaaa acgggaagtt atggattgaa caatatcaaa gaacgagtag ctggtattgg
     5221 tggaacagta aaaatcatta gttttaaagg tcaaggtaca agtgtagaaa tcaaggtccc
     5281 tttgatgaag gaggcataga taaggatgat aaaagtttta ttagtagatg accatgaaat
     5341 ggtgcgctta ggcgtctctt cttatttatc tattcaagaa gacattgaag taattggaga
     5401 agctgaaaat ggacgacaag gctatgagaa ggcgatgaca cttcgaccgg acgtcatttt
     5461 aatggactta gtcatggaag aaatggatgg tatcgaatca acaaaagcaa tcttaaaaga
     5521 ttggccagaa gctaaaatca ttatcgtaac cagttttatt gatgacgaaa aagtttatcc
     5581 tgccattgaa gcaggtgcag cagggtattt gttgaagacc tctaccgcac atgaaattgc
     5641 tgatgctata agggcaacgc aacgtggcga gcgagtatta gaaccagaag tgacaacaaa
     5701 aatgatggaa aaaatgagtc ggcgaaatga gcctgtatta catgaagaac taacgaatcg
     5761 ggaaaatgaa attttaatgc tgatttctga aggaaaaagt aatcaggaaa tagcggacga
     5821 attgtttatt acactgaaaa cagtaaaaac acatgtttcc aatatattgg ctaagctaga
     5881 agtggaagac cgtacacaag ccgctatcta tgcattcaaa catggtttgg tgaaataaaa
     5941 acgatgataa agaggataat caaaagctga gaaaataact attatttttc ttggcttttt
     6001 tacaataatg atgcttgctt ttgtttttgt aaaggctttg ttatactgag aaaagaagat
     6061 actaaaggag gaaaacggtg aaacaaaatt ttgcaattgt aggtcttgga cgatttggcg
     6121 gaagtatctg ccgaacactg atcgaagctg gtcaagaagt actggctatt gatagtaatg
     6181 aagatcgggt caatgaatat atgaatatag ccactcatgc tgttgtggca aatgcacagg
     6241 atgaaatgac acttcgatca ttaggcataa gaaatttcga tcatgtaatt gtcgctattg
     6301 gagaagatat tcaagccagt atcttagtta ctctgatggt aaaggaaatg ggcgtaccaa
     6361 atattttagc aaaagcccaa aatgagtatc atgccagagt acttgaaaag atcggagctg
     6421 atcgcgtcgt ccatcccgag cgagacatgg gaataaggat tgcgcataat ttagtgtcaa
     6481 agaatatttt agattaccta gagttgtcag ataaatattc tttagcagaa atccgagtct
     6541 ccaatcctaa atttttcaac aaaacattag ctgaattaaa tttcaggcaa cgttttgact
     6601 taacagtagt cgccatacga agagtagatc aaacggttat cgcttctcct gcagcagatg
     6661 aatatgttcg tgaaaatgat aatctactgg ttattggaga gacaggcgat gtggatattc
     6721 tagacgataa gatgaatcaa tagaaagaaa agagaaagta ggttgttggg atgaaattaa
     6781 cggtaacgcc aaaagcacaa aagtggtttg actcagaaat tgatctgcca gaaacttacg
     6841 gaatacgttt ttttggaaaa gtctacggca aaacagaggt tcatgatggt ttttcgattg
     6901 gaatgtcagt tgaacagcca gaacatcctg taaagaaagt ggaaatggat gggacacttt
     6961 tttttatcga ggaagcagat gaatggttct ttaaaggata tgatttactt gttgattatg
     7021 atcccaacct ggaggagcca acctatcact ttaaaaaaca ataaaaaaag agagggtgac
     7081 acaatttttg tcaccttctc ttttttcttt tgaaaatatt gttttcagct attaatatcc
     7141 tgcagacaat tttacttgat tggtttgaag ctcatgggtg gcaaagtagc ttttcagatt
     7201 ggttaaaaaa atagccatta atttcttttg aaaatcaggt gtcaaacctg caatgtggga
     7261 agtaatcagt acattgtcca ttttccaaag aggatgatct tgtggaagag gctcttcctc
     7321 aaaaacgtct aatgctgcaa aggaaagttg ctggttattt aaagcctgca ccagatcttg
     7381 tgtcttaaca gaagcaccac ggccaacatt gatgaataca gattttggat caaagtgttc
     7441 aaacatatgc tgatcaaaga gcccagttgt ttcctccgtt agaggtaaga tattgactac
     7501 gaaatctact tcaggaagaa cttcctttag ttgatttaaa gaaaaagtct tttccattcc
     7561 ttctgctgga tgaccggatg tattgattcc atagcagttc atgtctaatg ctttgcattt
     7621 ttttgcaatc atttttccaa tatggccagt tccaatgatt agtatttttt tcccttggat
     7681 agattggtaa tggaccgatg aacctaacca tttcatgtca ctctgtgcct tttgtgcttg
     7741 aaaaagacca cgagaatacc caaggatgat tcctaagaca tgctccgtaa tcgattcact
     7801 gtggatgcca ctaccatttg aaagtaaaat atgtttcttt gaaaaagtct caaggggtag
     7861 ctgatcaaca ccagctgaga tagattggac ccatttcaag gtagaagagg ctaatagttt
     7921 ttcttggtag tctgagttcc agcccaaact gatctctaca ttttgtagat cctctggttg
     7981 taattctgtt ttaaagatat aaccaggcgc tatagtatgg atttcttcga taaattcatc
     8041 acgaaatgga cgatctgcat aaatgattct ttctgacata gttaatttcc cgcctttctt
     8101 tactctaagt gtagcgcata caagaagaac tttgaagcgc cttgcttgtt ttttgagaaa
     8161 gaaatatttt tcaaataaaa aaggggctgt gacaaaaaaa tgtcacaacc tctaaaaggg
     8221 tctattgaag tagaaataac gagttgtctt tatttcttaa ttgatcgata tttttgccca
     8281 tcctttgaca gcttgagcaa aaataaagat tcctgcaaaa gcaatgaagg cgctagtgtc
     8341 aaaagaagga agaaagacca tcaagattgt tgcaattata gtaggcaatg aaattgaata
     8401 gatcaatgtt tttaaacaat caagaaaagt agcttttgtt agtcgaaagc gtgcatagag
     8461 aaaagcagca atgttggtaa atagcaaagt gatcagcaga ttcagaaaac taggataaat
     8521 agaaaatata aatgtcagga cttttatcca aagtggtata gatgcttcac ttaaaaaaga
     8581 acggagctgc ttacctgtta agttttttaa agattcattg gtgtagggaa gttctaattg
     8641 gttattgttc aacaaagcag aagtcgtgcc tgtattagga agagctatga taagtttgtt
     8701 ttttaacatc ccgacactaa caaaattccc catcaaatca gtagagatgt cctgttctgt
     8761 tcgttttccc tctggatcaa atgtgaagat aatggagttt gtttggtaaa tgaatccttc
     8821 tttttcttcg gtttgaagct gcccgttttg gattgtaaaa tcaggaatgc gagtagcaat
     8881 cttttgccca tccaattgaa tgttgtttaa gacttgaaaa acttgaaaag agattggcaa
     8941 agccataatt atactcaaaa ctaaaagata gacaatggct ttccaaaatg ggacatttct
     9001 cgcctccttt aggtctttga atcgtataaa ggaactgata attaattgtt ttgttgtcat
     9061 aagtactcct catctagtag tgtcatcact tgcaattcta gcgaagtatc aagtgaaata
     9121 caagtgcttt aaggaaacag ctgaatgatt ggtgaaatat caaaaaaatg ttattcttgt
     9181 ctaaagacta tccaatataa ataaaatgga ggaaggaata gtgcctacac agccacagag
     9241 atcatcaaaa aaactatttg gagtagtggt tttgaatgcg gtgatcactt tatcagaatt
     9301 tattggaggg attatgtcag ggagtttgtc tcttgtttca gatgctttcc ataaccttag
     9361 tgatacattg gcaattattt tttcttacgg ggcacaaaaa atggcccgaa aagaagcaaa
     9421 taaaaaacgt acgtatggct accagcgttt agaaatattg agtgctttca tcaactcatt
     9481 catattgatc atattatcct tgtttttaac cgttgaagca ttcaagcggt tcacttcacc
     9541 agaaaagata aatagtcatt tgatgctaac agtagcagtt attggattgc tcgcgaattt
     9601 gttctcagta ttattattac gacaagaagc agatgaaagt ttgaatataa aatctagtta
     9661 tttgcatttg ttaagtgata cactgtcttc ggtaagtgtg attatcggag caattttgat
     9721 ccgttttttc gatatctact ggatcgatcc agttattaca ctgatcattt cgatatatat
     9781 attgatagaa gcgataatgg tgatcaaaaa agctgccgct attttgatac aaagtgcacc
     9841 tgctatcgac tatgaaaaaa tggaacaaga aattgaagca atcgatgggg taaaggatgt
     9901 tcatcatgtc catatttggc agtatagcga aaagataatt attttcgacg gacatattga
     9961 ttttgaagat cagctactaa gcgagattga aaagatatat cctcgtataa tttctttatt
    10021 aaaattaaaa tatggtatta cacatgtgac gatccaagca gagacaaagg caaaggatca
    10081 aaaaaaatta atttttttaa ataaagatga aagttattga aatatagagt atgataaata
    10141 agaattttta ataaggtagg ttggctcatg aagcagtatc gacagttaaa aggatattta
    10201 gaagcaaaag gatattggga gatactagtc tatctctttt ttggtggttt ggctacgatt
    10261 gtaaattttg ttagttttgc tctcgcacgc caatattttg atttaagtat ggccttatcc
    10321 aattctattt cgtggttttg ttctgtattg tttgcttttg taacgaacaa actttgggta
    10381 ttccattcaa aatcaccgaa ttttacacat gcacttattg aatgcggtaa gttcttcttc
    10441 taccgtatcc tttcttacgg attggacatg ggggcaatgg tattgttgat caatgttatg
    10501 aacagcaatg agtatgtggc gaaaatcatc actcaaatta ttgttatcct tgcgaactat
    10561 attttcagca agttgtttat ttttaaagaa actgaggttt ttgaagaaga agtaggtcaa
    10621 agtgaaaaag acgactaggc ataacaattg aagaatttcc ttctctttgt tactctttgt
    10681 aatgtagaca ttattaagaa aacattgagg aggaattttt ttatgactta cacattacca
    10741 gatctacctt atgcatatga cgcattggaa ccttatattg acgaagaaac gatgcatctg
    10801 catcatgata agcatcacaa tacttatgtg acgaatttaa attcagcaat tgagaaatac
    10861 ccagaattag gcgaaaaaac aatagaagaa ttattatctg atatggacgc tattccaaca
    10921 gatatcaaga cagctgtacg taacaatggt ggcggacatg ctaaccattc atttttctgg
    10981 gaaatcatgg caccaaatgc tggtggcgaa cctacaggag aaataaaaga agcgattaat
    11041 gaagcttttg gtgatttttc ttcttttaaa gaagaattca aaaaagcagc cgctggacga
    11101 tttggttctg gatgggcttg gcttgtaatg gaaaatggaa aattagctat tacctctact
    11161 gcaaatcaag attctccatt gatggaagga aagacaccaa ttctaggttt ggacgtttgg
    11221 gaacatgctt attatttgaa atataaaaat gtccgaccag attatattgc agcattctgg
    11281 aatgttatta attgggatga agtaaacaaa cgtttcgaag cagcaaaata accatttcct
    11341 ccgtcatatc agtcttaaaa ataatttgga aaataaaaga gcgaaagctt gctagcgttt
    11401 tctaaattgg gtataatgtt cttgcaaggt tacttgcaag aacttttttg gaggatatac
    11461 atttatggaa cacggaacgg ttaaatggtt caacaatgaa aaaggatttg ggtttattac
    11521 agtagacggc ggagatgacg tatttgttca tttctcagca attcaaggag acggcttcaa
    11581 gagcctagaa gaaggtcaag aagtagaatt cacgattgtt gaaggtgcac gcggacctca
    11641 agctgctgaa gtgactaaat tataataatt tgttgaataa agacacgact tcttagagag
    11701 tcgtgtcttt ttgtctattt atgaagtttt tagatttttt ggctaagtaa atgcatttgt
    11761 tcacttgaca tgtcctaact aattatcaag caaggaacat tttcttcgtt agtaatggat
    11821 tttaaagaaa tttcagtttt tggatgttga ataaaagacc atttgtgaaa ccactagtat
    11881 acatactcat taccatgtca attctattac tttaaaagaa aatcgtttta ctttgaagag
    11941 gagcatatct atccattaaa agatgatggt aatgcaatca ctagttcgag tcattttaag
    12001 agccatgcag tgagctggaa ataaaacgaa tagaagatgg gtttaacaaa aggaccagtc
    12061 ataggaagaa attgtcgtga aatcgaatga taatctgaac ggtaggcaaa gactgttaaa
    12121 aagatgggaa aggtatgtgc gatcatttta ctgcacgatt atttggaaaa taccttgttg
    12181 ttcgcttttt ctggaaaaaa tacataaaaa aagataaaac gattgcgaac tgcgtgtatt
    12241 cggtctttcg
//
LOCUS       DACDKL010000076        12177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDKL010000076 DACDKL010000000
VERSION     DACDKL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.076"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(130..687)
                     /gene="frr"
                     /locus_tag="IV329_001581"
     CDS             complement(130..687)
                     /gene="frr"
                     /locus_tag="IV329_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8940758.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(690..1412)
                     /locus_tag="IV329_001582"
     CDS             complement(690..1412)
                     /locus_tag="IV329_001582"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8940759.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1548..2429)
                     /locus_tag="IV329_001583"
     CDS             complement(1548..2429)
                     /locus_tag="IV329_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8940760.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2528..3310)
                     /gene="rpsB"
                     /locus_tag="IV329_001584"
     CDS             complement(2528..3310)
                     /gene="rpsB"
                     /locus_tag="IV329_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8940761.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3669..4148
                     /locus_tag="IV329_001585"
     CDS             3669..4148
                     /locus_tag="IV329_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8940762.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4365..5456
                     /gene="phnW"
                     /locus_tag="IV329_001586"
     CDS             4365..5456
                     /gene="phnW"
                     /locus_tag="IV329_001586"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8940763.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5449..5577
                     /locus_tag="IV329_001587"
     CDS             5449..5577
                     /locus_tag="IV329_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8940764.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5581..6126
                     /locus_tag="IV329_001588"
     CDS             5581..6126
                     /locus_tag="IV329_001588"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8940765.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6580..8271)
                     /locus_tag="IV329_001589"
     CDS             complement(6580..8271)
                     /locus_tag="IV329_001589"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8940766.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8691..9638)
                     /gene="arcC"
                     /locus_tag="IV329_001590"
     CDS             complement(8691..9638)
                     /gene="arcC"
                     /locus_tag="IV329_001590"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8940767.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9753..10772)
                     /gene="argF"
                     /locus_tag="IV329_001591"
     CDS             complement(9753..10772)
                     /gene="argF"
                     /locus_tag="IV329_001591"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8940768.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10863..12092)
                     /gene="arcA"
                     /locus_tag="IV329_001592"
     CDS             complement(10863..12092)
                     /gene="arcA"
                     /locus_tag="IV329_001592"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8940769.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3331 a         2523 c         2201 g         4122 t
ORIGIN      
        1 ttcctatgaa atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt
       61 ttaaataaaa gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt
      121 atgattttat taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc
      181 tttgatgctg ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc
      241 ggtaatatca ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt
      301 acgaatcgcg atttttgcat tttctgcttc ttttttaaca tctttagcta attcttttct
      361 gcgttcttct gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact
      421 gattccgata tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa
      481 aggtgtgatc attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg
      541 tgttggcgca ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc
      601 tgcgcggatt tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt
      661 tgcttctgtc ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt
      721 tcgcctaaga ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga
      781 atatcattat ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca
      841 ataacttcca gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca
      901 gcagaataaa caccgtctac attgttttta gccatcagga taacatctgc gccgatttct
      961 gccgcgcgta atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca
     1021 aagataacga tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct
     1081 gcgatttgac gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac
     1141 gtatcctgta aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga
     1201 gcacgttcca tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca
     1261 acgattgcca tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg
     1321 attactggtg gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt
     1381 aagactacac gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc
     1441 attttaccat acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa
     1501 aaaagggagc gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact
     1561 tggctcataa cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg
     1621 aaacgtacga atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata
     1681 tcaggatctt taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag
     1741 cgtccttcta ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct
     1801 gtaagaacag ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa
     1861 cgaggattga tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt
     1921 ccatctagaa cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca
     1981 gcattgtcgt ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact
     2041 tgagttgctt cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca
     2101 gcttccatgt ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct
     2161 tggaacattt cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca
     2221 gtgttacctt tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc
     2281 gcagcctttg ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct
     2341 acttcaacta atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct
     2401 tttaccattt tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta
     2461 aaaaaagctg cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca
     2521 ttcaaaatta ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg
     2581 atgttgctgt gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc
     2641 cttcgatgaa agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt
     2701 cgtttgaagg gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta
     2761 cgattgggat atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa
     2821 cgatgtacat tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa
     2881 gacgttcacg ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc
     2941 catcttcttc cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc
     3001 agtttgtcaa tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg
     3061 cttcttcttt gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc
     3121 cttcttcagc aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt
     3181 gcaagtcaat gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat
     3241 tccagcgacg agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa
     3301 ttactgccat gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct
     3361 tgccggcaaa ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg
     3421 cataaagcac cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact
     3481 tttctgctca acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag
     3541 atttctcttt gtttctttat tatccttttt ccataaaata attttaggta taaaagacga
     3601 atattattgg gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga
     3661 gaaagaaaat gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc
     3721 cgatccgtac gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc
     3781 aggcaacgat ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg
     3841 gcgtttcccg cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag
     3901 aggatcggct ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt
     3961 tgacgatcgt caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga
     4021 tcaaacctcc ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg
     4081 aaaatgagga ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac
     4141 ttttttaatc agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt
     4201 tccttattga ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt
     4261 tcaacacctt gattgaatca caataatcat catttatatt ggatgttaac aagagcagct
     4321 tcttgctctt tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa
     4381 cttctggacc attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta
     4441 cttgggatca agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg
     4501 ctcaagtatc ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg
     4561 tagaatcggt attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg
     4621 gcgcatatag acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt
     4681 atgaggtatc ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga
     4741 atccgtctat ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata
     4801 atttggaaga gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga
     4861 tgtcaagctt tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt
     4921 ccagctcaaa caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag
     4981 cggaactgga aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt
     5041 accgcgtgat ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt
     5101 tggcttttca acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt
     5161 cacgttatct cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc
     5221 ctttcattca agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc
     5281 gatttgattt taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg
     5341 ggaaaatcag cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag
     5401 atattttgga actgacagca attatcaaag actataagga gaacgttaat gattaaaact
     5461 gtaatttttg attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct
     5521 tttagaaatg cttttctaga aaaaggaatc caactgactg acaaagaaat ccgctaaccg
     5581 atgggaaaat taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa
     5641 caatggatag ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa
     5701 caatttgaac attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca
     5761 ttggaaacga tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt
     5821 aatgctccta tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac
     5881 ttcatggcaa cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat
     5941 aaaaacatgc agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg
     6001 gtctcagata tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt
     6061 agttcgctaa tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc
     6121 gtttaaaaca gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca
     6181 atttaaaaga gttaacagaa tacattttgt acgctgacat gaatccagaa tgacttttat
     6241 acaaaataag aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa
     6301 aatagctcct catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac
     6361 accgtttatt cgaaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt
     6421 tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact
     6481 ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta
     6541 atactcggat cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg
     6601 aagtcctagc aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa
     6661 gcgtgcttct ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa
     6721 tgcttgtgct aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc
     6781 agacaagacc gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc
     6841 attcaatgca tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac
     6901 tgcacgagca tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc
     6961 ttccaggtta aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc
     7021 cccaacccca acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct
     7081 gatttgttct ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt
     7141 tcctttacga gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg
     7201 catgtcgtca gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg
     7261 atagctttgt tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg
     7321 gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat
     7381 caacgctgga ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt
     7441 cagcaaatgt ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc
     7501 ttcaccgttc aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt
     7561 catcgattca tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag
     7621 acgtttgaac caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg
     7681 gaattggacg tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt
     7741 accccatttt ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg
     7801 gttgatgcgg attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat
     7861 aactgtcgaa cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc
     7921 gattggtact gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac
     7981 tactgtttgc aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg
     8041 tccgacaact tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc
     8101 gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc
     8161 tccgtgttca gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc
     8221 atctttgaca gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc
     8281 tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta
     8341 tgccatcact agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca
     8401 gctccatctg atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc
     8461 gttgtatctt cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa
     8521 gtactctctt gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa
     8581 aaaacgaaag gctagtcgtt tcataaaaaa acaatccaga tggcctaaag gagaagaaat
     8641 accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct
     8701 acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg
     8761 attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcttctac tttcggaagc
     8821 atgcttcctg gagcgaattg cttttcatca atgtattgtt tcatttctga aacagtaaca
     8881 gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc
     8941 aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta
     9001 tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg
     9061 ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct
     9121 ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct
     9181 ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat
     9241 ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga
     9301 gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggcg
     9361 ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt
     9421 gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga
     9481 ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt
     9541 gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagatggca
     9601 ttgcccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt
     9661 ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga
     9721 ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct
     9781 aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga
     9841 cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt
     9901 tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag
     9961 aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt
    10021 ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca
    10081 gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt
    10141 gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat
    10201 tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag
    10261 ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca
    10321 aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc
    10381 aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg
    10441 aatccacgga attcgattcc gtcaaacata cgtcctagta ctttagctgt atcttctgtg
    10501 gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga
    10561 tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc
    10621 gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag
    10681 tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat
    10741 aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt
    10801 atttaagatt aaagaggaaa tcccgacggc agctgacagt aagactgccg tcaggaaaca
    10861 aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacgacct
    10921 cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg
    10981 ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt
    11041 gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc
    11101 agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga
    11161 gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta
    11221 ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc
    11281 aaatgcatca ttgcacggtt gttcggaatt ttgattgcca acactttttc aaatcctgaa
    11341 ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa
    11401 atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct
    11461 ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc
    11521 aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact
    11581 gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg
    11641 tccatgtaaa atggatagtc atcgtctgct gacagatcat atagactgct agaacgtact
    11701 tcgatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt
    11761 gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc
    11821 aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat
    11881 ttttccaagt aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa gttgtcatgt
    11941 tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct
    12001 ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta aaactgtctt taatttccct
    12061 atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac
    12121 atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaatg
//
LOCUS       DACDKL010000077        12057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDKL010000077 DACDKL010000000
VERSION     DACDKL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.077"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..657)
                     /locus_tag="IV329_001593"
     CDS             complement(<1..657)
                     /locus_tag="IV329_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8940770.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     Q"
     gene            791..1078
                     /locus_tag="IV329_001594"
     CDS             791..1078
                     /locus_tag="IV329_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940771.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1494..3032
                     /locus_tag="IV329_001595"
     CDS             1494..3032
                     /locus_tag="IV329_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8940772.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3029..3994
                     /locus_tag="IV329_001596"
     CDS             3029..3994
                     /locus_tag="IV329_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940773.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3981..5303
                     /locus_tag="IV329_001597"
     CDS             3981..5303
                     /locus_tag="IV329_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8940774.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5366..5470
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5544..6287
                     /locus_tag="IV329_001598"
     CDS             5544..6287
                     /locus_tag="IV329_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940775.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6302..7294
                     /locus_tag="IV329_001599"
     CDS             6302..7294
                     /locus_tag="IV329_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8940776.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7306..8043
                     /locus_tag="IV329_001600"
     CDS             7306..8043
                     /locus_tag="IV329_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940777.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8033..8755
                     /locus_tag="IV329_001601"
     CDS             8033..8755
                     /locus_tag="IV329_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8940778.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8839..9462)
                     /locus_tag="IV329_001602"
     CDS             complement(8839..9462)
                     /locus_tag="IV329_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8940779.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            9797..10231
                     /locus_tag="IV329_001603"
     CDS             9797..10231
                     /locus_tag="IV329_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8940780.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            10254..10574
                     /locus_tag="IV329_001604"
     CDS             10254..10574
                     /locus_tag="IV329_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940781.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            10715..10906
                     /locus_tag="IV329_001605"
     CDS             10715..10906
                     /locus_tag="IV329_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940782.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(10977..11234)
                     /locus_tag="IV329_001606"
     CDS             complement(10977..11234)
                     /locus_tag="IV329_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8940783.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            11392..11655
                     /locus_tag="IV329_001607"
     CDS             11392..11655
                     /locus_tag="IV329_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940784.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            11679..11867
                     /locus_tag="IV329_001608"
     CDS             11679..11867
                     /locus_tag="IV329_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940785.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT         4079 a         1998 c         2526 g         3454 t
ORIGIN      
        1 ctgttcaaga ggatcaagtg gttcaaatgt ttctgtagtc tgttttgctt ccaattgggg
       61 caaaacagat ccaatttcca gatacttcac tttattcaat gtataagata gaatagaaca
      121 gctaagtaat gtatacagaa aatgtgtgag atccaaataa agcaaggaga caactaaaat
      181 accaaaagta atcaagaaaa gacttttgcc aatcttaata tgaaaatatt tttcaatggc
      241 ttgataaatg atatctgttc cgcctgtgct tccgccgaat cgaaagacga gaccgcaacc
      301 aatgccagaa agaatacctg tcatcaaact attgatcagt agactatcta cagcaaaatg
      361 tatatgaagg ctctcccata aatgtaaaaa gaaaactagc gcaaatgttc cgacgatcgt
      421 ccggagaaaa gcgtgttttc caaaaagaaa caatgagata agcaataaag gtaaattaaa
      481 aagcagtgaa gtgacagaag gagcgaatcc aaaaagatta tgcaaaagca aagcaatacc
      541 agtaacccca ccatctgcca gcttagatgg aatattgatt cctgtcacag ctaatccgta
      601 gattcctgcg cctaaaatga ttccgcacaa ttctgtaaat aaatttaatt ttttcatttc
      661 tagcctcttt tcttttgaaa attttcagca acttctttat tatactgact tatactctta
      721 cttcaagagg aaaatgaaaa aaaattattt ttggctgttt ctttttattt atcaaataag
      781 gagtggaaaa atgcgagcag cagacttatt gacaattctt acaaaatcac ccaaaaaaac
      841 agagtatcaa ggagtttttt tggctcaaga acaaaaactg gtgcctgtga caatggtcca
      901 gataaataaa gcaaatcaat ttatcttttt atttgaacac ggcaaagcac cacttgctat
      961 gaaagacatc tttctttttt tgatgaagaa ccgtgagaaa gctgtttatt atcagtcaaa
     1021 agaagaactc attcctcttt atggagtgaa agagatagaa aataaagtgg tgatataagt
     1081 aaaaataaga aaacaagacg tttatgatgt caaaacaacc attttttaca tttttggcaa
     1141 aaaagtgtca tgatacagta taatattcgt tgaacaggcc aataaaggtg ttctccccca
     1201 cgagatttct actttattgg ctgtttcttt ttattaaaat aaaagttgtg tgtgtaaaaa
     1261 agatatagct gattccaagt gacaatttcg tatagatttt tatggtattc gtagtgaaaa
     1321 aacgagaacc aaaaagttga tccgactttg ttgcttggat tctttttttg tcagcatatt
     1381 tagaggtatt tttttctttt ttttgttcgt gctgtaaggt taaggcaaga caagttagtt
     1441 gggaaaaagg aaagaaatgt ttatacttaa agaataacag aaaggaaggt cgcatgaata
     1501 attttataca aacattaagt gaaagaaaag gcgagttgct ggaagctgca tttcaacatc
     1561 tgtccatttc tctcatttct ttgctgattg cagcactgat tgctattcct ttagcgattt
     1621 gggcagccaa tcataaaaaa atggcagaag tcttattgca gatcacgggt gtgctccaaa
     1681 cgattccttc acttgcctta ttaggtttac ttatcccatt tgtagggatc ggaactgttc
     1741 cagcattgat tgcacttgtt atttacgctt tgcttccgat tttccaaaac acgtatattg
     1801 gtctagctga aattgacccg tctattgaag aagcagcggt agcatttggt atgtccagaa
     1861 tgagacgctt gctgaaagta gagcttccca tcgcattacc tgtcattatc tcaggtatcc
     1921 gaactgcctt agttttaatc atcggtacgg ctactttggc agcattgatc ggagcaggtg
     1981 gattagggac atttattttg ttaggtattg accgaaatac acctgttttg acattgattg
     2041 gtgcgatcag ttctgccttg cttgcaatta tattcagcgg actgattcgc tggttgcagc
     2101 ataagaaacc tcgatattcc atcattactt tagcagcagt ggtgtgttta tttggcggcg
     2161 tttctttata tcagtctgcc ttgtttaaaa atgaaaccat cacgattgct ggaaaattag
     2221 gagcggaacc tgaaatcctg atccaaatgt ataaagaatt gattgaagac aacacaaaaa
     2281 cagaagttga attaaaaccg aattttggca agacgagttt tttgttcaat gcactggatc
     2341 aaggtcaaat cgatatctat cctgagttca caggtactgt cctagaaagc ttggtgaagg
     2401 tacctgaagc aatgaaaagt gctgatttga caaaagaaaa aacatatgac gaagcagaag
     2461 aattattgaa taagcaattc gatatgacat tattaaaacc aatggcttac caaaatacgt
     2521 atgccttggc agtcaagaga tcttttgcga acgaacatca acttaaaaag atttccgatt
     2581 tggcagctat ctctgatcag atcaaagcgg gattcacact ggaatttatt gatcgttcag
     2641 atggatataa aggaatccaa gaagcatatg gcttgaattt ttcaagtgta caaagcatgg
     2701 agccaagttt gcgttaccaa gcaatcaata atggagatgt caatattgtg gatgcctatt
     2761 ccacagatag ccagctcaag gagtacgatc tagtgactct agaagatgat cgagggttat
     2821 tcccagctta tcaaggcgca ccgctgatga aaaaagattt tgctaaagaa catcctgaaa
     2881 tcgttaaagc actgaataaa ttaagcggaa aaataacgga ggatcaaatg attgatatga
     2941 attaccaagt caatgtcgaa aagaaaaagc cgacagaagt agcacgaatc ttcctgaaaa
     3001 atgaaggatt agtaaaggag gaagatcagt gactaaatta atcgagttca aacatgtgca
     3061 aaaaaagtat gatggaaagt atgtcatcga tgacctaaat ctggcaatca aaaaaggaga
     3121 aatctttgtt ttggttggtc cttccggtag tggaaaaaca actactttga aaatgatcaa
     3181 tggattatct aaaccatcag caggagacat ctacttcaaa ggaaaatcgt taaatgaata
     3241 taatctgcag aaaatgcggt ggaatatggg atatgtgctg cagcaaatcg ctttgttccc
     3301 gaccatgaca gtcaagcaaa atattgaagt catccctgaa atgttaggtt gggaaaagca
     3361 gaaaagagca gaccgtgtag atgaacttct tcaaaaagtt ggcttatctc ctgatattta
     3421 tcgagatcgt atgcctagag agttgtctgg aggagaacag caacggatcg ggatcatccg
     3481 agctattgct gcaagcccag atgtgatttt gatggacgag ccatttagcg cattagatcc
     3541 gatttcaaga aactcattac aagaattggt tttgtcctta cacgaagaat tgggcacgac
     3601 catcgttttc gttacgcaca atatggaaga agcaatcaag ctaggtgatc gtattgcatt
     3661 tatgaaggat ggagaaatta tccaatgtga cacaccagag cagttattga tgaatcctaa
     3721 aaacgactac gttcggcatt tctttgatga accgaaacag acgaaagagt ggcgagtaga
     3781 agatttagtc gtcaatggat atttcttaaa tgaaatacca gaaaatgctc gtcaacaagt
     3841 ttgctttgat acgcctatga aagaagtata ttcgcttctt tcggtgtatc caagcattgt
     3901 gatagtagaa aagcaaagaa gtattgggtc gctgacaagt aaagaaatct ttgctttttt
     3961 aagtcgagag gaggaaggaa atgaaaacat atgatgcaat catcatcggt tcaggtgtga
     4021 gcggcttaag tgctgcttat ggattgaaag aagcaggtaa aacagtacta gtggtcgaag
     4081 aagatctatg gggcgggact tgtcctaacc gtggatgtga tcctaaaaaa gtcttgttaa
     4141 gtgcagtaga ggcacgtaat cgggtaaaac aattgagtgg aaaaggtttt aacgagatcc
     4201 caactgccaa ctgggaagaa cttcaaaaat tcaaacgaac atttacggat ccagtcccag
     4261 aaagccgaaa aaaacaatta gcagaagctg aaatcgatca tctttcagga accgctcgtt
     4321 ttttggatga ttcatctatt gaagtcaacg aagaagtctt ccatgcggat tacttagttt
     4381 tggcaaccgg gcaaagacct acgatcttac cagtggaggg aaaggaatac ttgaaaacta
     4441 gtgcagactt tttatcattg cctgtactgc ctaaagaaat catttttatc ggtggtggtt
     4501 atattgcttt tgagctagct acgattgcca atgctgcagg gagcaaagta accattgtcc
     4561 atcataatca acggccatta aaagaatttg aggcgtcttt ggtagaggaa gccgtccatc
     4621 aaatggaagc atctggcatt cagtttgctt ttggtgttga aacccaaaag atcatttcag
     4681 aaggtactcg ctatcgttta gtcggaaaag agacggaatt agtagcagat atgatttttt
     4741 gtgcgactgg gagacagccg aatacagaat ctttagcact tgagcaagcg aatatcgtct
     4801 ttgataaaca cggaattgca gtcaatgatt atctgcaaac gagcaatcca aaaatttttg
     4861 cgtgtggaga tattgtttca aggaaaaccc caaaattgac accagtcgcc acttttgaag
     4921 gaaattatgt agctaagcgg ataacggatg caacaagcga acctatcaaa tatccgatta
     4981 tacctacgat cgtctacgcc agtcctaaac ttgctgaagt aggagttaca aaaagtcacg
     5041 cctcttcgtc agatcaagtg gtagagatgg atcttaccag ttggttcacc tatcatcgtg
     5101 tcaatgaacc agttgctaag gctgaattga cctttgacca gcaaaattat ttgattggtg
     5161 ctgcagtgat tagcgagcaa gcagacgaat tgatcgatga tttgacactc gtcatcaatc
     5221 aaaaattgac taaaaaagag ttggatagct atatcatggg gtatccgaca ttggcaagtg
     5281 atctctccta tttattgaaa taatcagcat gataactaaa gcgcttgttg ctttttcctt
     5341 cttgtgctac actttaaaaa atgaatacct gtttctgggg atgtcgtaga cgacatgaga
     5401 gtaagttgtg aacttagacc cattgaacct gtcagttaat actggtgaag ggagaaacat
     5461 agcagacgct tgaagcgggt tactatgtcg tgcccatttc aagcgttttt tgttattcat
     5521 attagaagaa aggaggtaac aaaatgaaga aaaatcttta tccagcagtc agtatggcat
     5581 tcattttaat tatttggcag attgttgtca tagtttggaa aatacctgcc tttattttac
     5641 ccggacctgc tgctgtattt tctgcattag cggcagatgc acaagtatta ctttcccatt
     5701 ctgtcacgac attaaatgaa gcagtgcttg gtttggtaat agctagcgtt ctttctttta
     5761 ttactgcctt ggcaatggat tattggaagt ttttagaaat gtctttttat ccgttactag
     5821 tgatttcaca aatgttgcca atcatggtgt tagggccatt acttacccta tggtttggtt
     5881 tcggtatgct tccaaaagtg atccttgttg tcttgataag ctattttccg atcgtagttt
     5941 ctttttcgga tacattgagg aaagtatcaa aagagcaaat catctttttg aaaacgatgg
     6001 gggcagatac agggaagatt tatcgaatat ataaaatacc agcgggttta tccggcttct
     6061 tttccggttt aaaagtggct gctacttatt gtgtcagcgg agcagtcgtt ggagaatggc
     6121 taagcgctca agcaggctta gggtattaca tgatacgcgt aaaaaacagc taccagatcg
     6181 ataaagtttt tgctgcgatc atttgtgtca ttctattgag tttattgctg aatggcgctt
     6241 gttcattatt gaaaaaaggg tattatcgta tgctttatat tttttaagag aaggggatca
     6301 aatgaaaaaa ttatggcttt tattaccgct actgttgctt ttatccgctt gtggcactgc
     6361 aaaagaaaca agtagcaaac aagaaataaa ggatttgaaa aaagtgacac ttgttttgga
     6421 ttatgtaccg aatactaatc atactggcat ctacctagca aaagaaaaag gctattataa
     6481 agaggctggt ttaaatgtcc aaatcatcga accaggagat aattcgacaa gtattggtct
     6541 agtaggtgcg gacaaagcac aatttggtgt aagttatcaa gaagatgtca cttatgctca
     6601 cgcagatgga cagaatatac cagtcaaagc aattgctaca gtgatcaaac ataatacttc
     6661 tggattcgcc accctatcag acagtaacat ccattcacca aaagattttg aaggaaaaac
     6721 atatgcaggt tggcaaagtc caagtgaaga agctgtctta aaagctgtga tggaaaaaga
     6781 cggcggggac ttttcaaaac tgacaatggt aggaagcaat ggagaagggc cagaaagctt
     6841 aggaaaatcc tcagatatcc aatggtactt tgaaggttgg gatatgatca aagcaaaaga
     6901 agcgggtatt gaactaaact atatcccatt gaaagaatta gatgaacggt tagattacta
     6961 tactccggta attattacga atgatcaact gatcaaaagt gatccagaat tggtacagtc
     7021 ttttatggat gccacgaaaa aaggctatca ggaagctatt aaggatccaa atgacagtgc
     7081 taaactgttg caaaaatatg caaaagaaaa tgatcggacg tttttagaag aatcacaagc
     7141 attcttgtca aaaaattata cggatgatcc tgagaattgg ggattgatgg aagaaaaagt
     7201 ttggcgcaat tatacagctt ttatgcagga aaacggattg atcaagcaag atgtaccttc
     7261 gcaagaacta ttcactaatc agtttataaa ataggagtga tagatatgtc aatccaaatc
     7321 agccacttga atgtagtcat tgaaaacctg aaaatattag aagacatcca tttcactatc
     7381 cctaaagata cctttacatc gattgtcgcg ccaagcggag ccggtaagtc aacattgctg
     7441 aaaactttaa caggagtcca gcctatgact tctggaacga tcaaagtaga cgatcaaaaa
     7501 gtaactggtc tccatacagc atttagttat atgccgcaag aagatatgtt acttccttgg
     7561 ctgagcgtct atcaaaacgt tacgttatat caaaaaatca atcatctaac aatcaatgaa
     7621 gaacaagtaa gggattatct ggatatcttt gggttaaacg gctatgaaca ttttttgccg
     7681 gaacaattat ctggcgggat gaagcagcga acggccttgt tacgaacaat catgaatcct
     7741 tcttcttact tgcttcttga tgaaccattt ggagcgttag atgctatgac cagagggcag
     7801 atgcaagact ggatgttgaa gctgcctaaa aaagcaaagc ggactacttt acttgtgacg
     7861 catgatatcg aagaagcaat ttatttatca gacaggattc ttgtgctttc cgcccgtccg
     7921 gcacatgtga ttgctgagat tcaagtgcct gaaaaacgac ggtcacgtga atggctactg
     7981 cagcaatcag agttgaagca aacgatttat cagttattgg caggtgaatt agatgttaag
     8041 tgatgctcat tgtcatttag atgggagtca gagtttagca ctccttcagc aggaacacac
     8101 gattttgacg atcatcaatt gtgattcgcc agaagaatgg aaggaaaaca gacagcttgc
     8161 agcaagtaag actcaagcct taagttacgg tatccatcct tggaaagcag atagctatac
     8221 gtttgaacaa gttgagcctt ttttgaaaaa agcaaggatc atcggtgaaa tagggttaga
     8281 taatatttgg acgaatgtac cgatgactac acaaaaaaaa gtatttgagc gacagcttgc
     8341 ttttgcagcc ataaatgaga aacctgttgt actgcacaca aaaggatgcg aaaaagagat
     8401 cttaaattat atccaagctt accctaatca ctatttgatc cattggtatt cttctttgga
     8461 ctatcaacaa gactatattg atttaggctg ttatttcagc attggtcttg atttgaaaaa
     8521 gaatccagca gtctggcaac tagctaaagc agtcccaatc gaccacttac ttattgaaac
     8581 ggatggacta catgcagcag agtgggcttt aggggggatt ttttcagaaa aagattatgt
     8641 ctcactgctt gaagaacagt taaagacaat cgctgagatc aaagagctat cagttgaaga
     8701 agttaaaaaa aatacttatc aaaacttgga acgtttcatt agattaggta aataaaaaag
     8761 agatgtgaca caactagtgt cgcacctctt tttggctttc tttgtataaa agtaaactgt
     8821 ttgattaata aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc
     8881 ccaagaacca taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga
     8941 gtagtcacca tttaagtaag aagaatctaa ttggtaacgt ccgatgtaac gtccgttcgt
     9001 tgctgtgtaa gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtgct
     9061 tgctggagta gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc
     9121 tgcttgtttt tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt ggactggtgc
     9181 ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca gagccttctg ttgggattgt
     9241 taattgttgt cctgagtaaa tcaggttaat atcagaaatt gaatttgatt ctgcaatggc
     9301 attgatcaag gagttatcac caacgtattt ttgagaaatc gttgatagag tatcacctga
     9361 ttgaacggta taagcttcgt ccgcatgagc agtcgttccc atgaagaatg cagcgccggc
     9421 agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc atgaaaaatc tcctttattc
     9481 gttaaaaagt tttatttgtt gtgttcaacg aggaatactt taacacgtga aaatatttca
     9541 taggtaaaag aagcatgaca atttatgaca aatccacgtt ttttgtaaca gaatgatgtt
     9601 cgctcgatgt gaaaaatgat gaggaatttc acataatgca gaaataatgg gattagaaca
     9661 ggtgttctga tgagaaaaga aatgtataaa taacttgaaa gaaattgctt ttgttcatca
     9721 ttttatttac atattgcgta tattttttgt atttttgctt gagatgttac aataaaagaa
     9781 aaaggaggcg gttccaatga gtaaaagata ccagaacatc atggtagcag ttgacggatc
     9841 aagacaatcg atcaaagcgt tcgctgaagc ccttgactta gcaaaggaca atgaagccca
     9901 cttatttatc gtgtcaatca tcaacaaagt tgagctgacc catagtgcct atgccttttc
     9961 aaaaatctat gcagatgaaa aacaaagaac agaagtagcc atgctgaaaa aaatcaatga
    10021 tgccaaagag tacggcattg ctgagatcca cgcaatagtt gaaactggag atccaagaaa
    10081 tttgattgcc aatgtcatcc ctggacaaga aaatattgat ttgatcgtta tgggagcgac
    10141 aggaaaaggt gctatacaac aagcattagt tggatcgacc gcttcttatg tcgtatcaca
    10201 tgccccttgc agtgtgttgg tcgtgaagta atgagataag ggaggaatag aaaatggttc
    10261 gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat tttagcaatt attgaagacg
    10321 gcacacaaac aaagaaggga tactcgatcg ctttagtaaa gtggaaagga aaaaaaggtg
    10381 ttgctctgag atgggatgga gataaccaac aagataaagg attccctgtc acagctaacg
    10441 gttatcatcc agcgtggttt gttctgcctg ataaatttat ggaattatac agctatgatt
    10501 atgcaagagc cgcagcatca atcaaagcat tagataaatt aacagaagag cagaatgaaa
    10561 tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga aaactgatta gttcgaggag
    10621 taaaaatgaa aaaaggttat ttgggatgaa actagaataa tgcgtataac ttaaataaaa
    10681 cttaaagcaa taaaatggca agagggtgag gcaaatggaa gcaggaaaag tattacagga
    10741 agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg aaaagcctga tcgaaaagaa
    10801 caaagaaaat ttggaagaaa agtacccgat cgcacaagaa tttatagaat taaaacaagg
    10861 aacgatcctt caccgattca aacatttttt tggtataaca gaatagaaaa aacgattaaa
    10921 gatcggcaga aatttgctgc atctttaatc gttttctttt taaacatgcg agggattcaa
    10981 ttctgattct tatataaaaa atagatccaa ataataagaa aaattactga aagcgtcaat
    11041 tcgatgatgt gtgttattaa tacagacgtt gtttcccaat gcctaaaaga aaaaatttga
    11101 taagtaaaat atacagcagg taaagaggcc agtaaaactg aaaacttctt ttttgtccat
    11161 tttatgtaat agaaaagaat gcttatcata ataaaaaaag gaatataaaa aacaaattga
    11221 ccgatcgcga tcatactatt gcctccgata aataaagtcc atgatacaaa ataacacttt
    11281 gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta aaatctgtat cgattttgaa
    11341 attagaggag aaacttgcta taatcaaagt gaaagaagga gtgggatttt catggatcat
    11401 gtagtgttag aaatcgattt gcatttgatc aataatattc gggtgattta ctatgttgta
    11461 agtaacagtg tcgaacaacg tgtactgacg aataaaataa atggtatttt agcaaagaaa
    11521 gacgttcacc gattcaataa tggtgaagga agttattata gtatcccagt agaaaaaatt
    11581 atttatacga cagtcaaagt cagagaagat ctagatacca aaaaagcata tgaacctatt
    11641 tttacaactt attgattgac agcagatgga ggaaaaaaat gaatccatat gaacaactta
    11701 ttcaagattt gatcgatgga aaaatcgaaa agattgatgt aaaaagagaa gaattaatgg
    11761 cttttcgaga agcgtggctg aagttggaga atcgtaaata tgtggtggga aaagcctcac
    11821 tgaacggcaa tgtgacttat cattacgatc cgacacgttt attttagtat gaagtattca
    11881 gaaaagaacc aaaggaggac gatgcagcag cattgtcctc ctttgatttt acaaaagtga
    11941 aagttttact tatttgaata gatttttaaa tttgtttttg acactgtcga tatttacatc
    12001 ttttaaaaga ttttgggctt tctctttata aggtcctaat tgttctttat gttcttc
//
LOCUS       DACDKL010000078        11668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDKL010000078 DACDKL010000000
VERSION     DACDKL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.078"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IV329_001609"
     CDS             complement(37..216)
                     /locus_tag="IV329_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940786.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IV329_001610"
     CDS             complement(597..1304)
                     /locus_tag="IV329_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940787.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IV329_001611"
     CDS             complement(1297..1734)
                     /locus_tag="IV329_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940788.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IV329_001612"
     CDS             complement(1749..2000)
                     /locus_tag="IV329_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940789.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IV329_001613"
     CDS             complement(2000..2206)
                     /locus_tag="IV329_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8940790.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IV329_001614"
     CDS             complement(2643..2903)
                     /locus_tag="IV329_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940791.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IV329_001615"
     CDS             2978..3181
                     /locus_tag="IV329_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8940792.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IV329_001616"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IV329_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8940793.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IV329_001617"
     CDS             complement(3810..4709)
                     /locus_tag="IV329_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8940794.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IV329_001618"
     CDS             complement(4722..5315)
                     /locus_tag="IV329_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8940795.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IV329_001619"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IV329_001619"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8940796.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IV329_001620"
     CDS             complement(7771..9231)
                     /locus_tag="IV329_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8940797.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IV329_001621"
     CDS             complement(9292..11664)
                     /locus_tag="IV329_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940798.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtcc
//
LOCUS       DACDKL010000079        11508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDKL010000079 DACDKL010000000
VERSION     DACDKL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.079"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(110..655)
                     /locus_tag="IV329_001622"
     CDS             complement(110..655)
                     /locus_tag="IV329_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8940799.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(667..1359)
                     /locus_tag="IV329_001623"
     CDS             complement(667..1359)
                     /locus_tag="IV329_001623"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8940800.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1378..1662)
                     /locus_tag="IV329_001624"
     CDS             complement(1378..1662)
                     /locus_tag="IV329_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940801.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1675..2238)
                     /locus_tag="IV329_001625"
     CDS             complement(1675..2238)
                     /locus_tag="IV329_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8940802.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2466..3128
                     /locus_tag="IV329_001626"
     CDS             2466..3128
                     /locus_tag="IV329_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8940803.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3252..4448
                     /locus_tag="IV329_001627"
     CDS             3252..4448
                     /locus_tag="IV329_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8940804.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4549..5925)
                     /locus_tag="IV329_001628"
     CDS             complement(4549..5925)
                     /locus_tag="IV329_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8940805.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5962..6828)
                     /locus_tag="IV329_001629"
     CDS             complement(5962..6828)
                     /locus_tag="IV329_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8940806.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6917..7942)
                     /locus_tag="IV329_001630"
     CDS             complement(6917..7942)
                     /locus_tag="IV329_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8940807.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7960..8634)
                     /locus_tag="IV329_001631"
     CDS             complement(7960..8634)
                     /locus_tag="IV329_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940808.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8818..9711)
                     /locus_tag="IV329_001632"
     CDS             complement(8818..9711)
                     /locus_tag="IV329_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8940809.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9785..11482)
                     /locus_tag="IV329_001633"
     CDS             complement(9785..11482)
                     /locus_tag="IV329_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8940810.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3413 a         2522 c         1972 g         3601 t
ORIGIN      
        1 taagtttttt taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct
       61 cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt
      121 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
      181 ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      241 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      301 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      361 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      421 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      481 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      541 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      601 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      661 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      721 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      781 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      841 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      901 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
      961 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
     1021 tggtaattgt cctggtttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
     1081 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1141 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1201 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1261 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1321 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1381 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1441 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1501 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1561 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1621 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1681 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1741 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1801 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1861 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1921 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     1981 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     2041 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccacc
     2101 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2161 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2221 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2281 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2341 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
     2401 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2461 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2521 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2581 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2641 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2701 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2761 agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt
     2821 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2881 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     2941 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     3001 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     3061 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3121 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3181 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3241 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3301 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3361 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3421 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3481 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3541 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3601 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3661 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3721 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3781 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3841 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3901 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     3961 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     4021 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     4081 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4141 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4201 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4261 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4321 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4381 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4441 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4501 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4561 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4621 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat
     4681 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4741 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4801 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4861 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4921 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     4981 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     5041 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     5101 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5161 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5221 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5281 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5341 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5401 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5461 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5521 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5581 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5641 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5701 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5761 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5821 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5881 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     5941 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa
     6001 aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc
     6061 aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc
     6121 aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa
     6181 taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa
     6241 gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa
     6301 attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc
     6361 tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc
     6421 ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac
     6481 ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat
     6541 taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg
     6601 gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat
     6661 ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc
     6721 gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc
     6781 aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct
     6841 tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt
     6901 cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag
     6961 tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg
     7021 ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat
     7081 ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc
     7141 ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt
     7201 ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga
     7261 tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt
     7321 tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat
     7381 attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt
     7441 ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa
     7501 tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat
     7561 ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta
     7621 cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg
     7681 tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc
     7741 cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct
     7801 caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca
     7861 tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt
     7921 tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg
     7981 tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg
     8041 attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg
     8101 cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg
     8161 atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac
     8221 attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga
     8281 acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac
     8341 aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga
     8401 gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc
     8461 caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga
     8521 gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc
     8581 catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa
     8641 tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc
     8701 ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc
     8761 gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta
     8821 tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa
     8881 aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt
     8941 ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac
     9001 tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg
     9061 cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac
     9121 ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc
     9181 ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg
     9241 tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa
     9301 caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat
     9361 ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc
     9421 ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt
     9481 ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc
     9541 aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc
     9601 taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg
     9661 taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac
     9721 cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg
     9781 ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca
     9841 aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc
     9901 cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt
     9961 cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa
    10021 gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt
    10081 gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc
    10141 actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata
    10201 ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac
    10261 tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct
    10321 ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt
    10381 tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat
    10441 ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt
    10501 ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt
    10561 ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa
    10621 ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag
    10681 aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta
    10741 gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa
    10801 ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca
    10861 aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca
    10921 tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag
    10981 ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg
    11041 tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac
    11101 ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg
    11161 cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat
    11221 cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac
    11281 caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt
    11341 ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta
    11401 gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg
    11461 tgacaatcat ttttttattc atttttttct ccccgttctt tttttatt
//
LOCUS       DACDKL010000080        11414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDKL010000080 DACDKL010000000
VERSION     DACDKL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.080"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            363..1355
                     /locus_tag="IV329_001634"
     CDS             363..1355
                     /locus_tag="IV329_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8940811.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1371..2255
                     /locus_tag="IV329_001635"
     CDS             1371..2255
                     /locus_tag="IV329_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8940812.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2304..3911
                     /locus_tag="IV329_001636"
     CDS             2304..3911
                     /locus_tag="IV329_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8940813.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3912..5396
                     /locus_tag="IV329_001637"
     CDS             3912..5396
                     /locus_tag="IV329_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8940814.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5573..6565
                     /locus_tag="IV329_001638"
     CDS             5573..6565
                     /locus_tag="IV329_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8940815.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6617..7486
                     /locus_tag="IV329_001639"
     CDS             6617..7486
                     /locus_tag="IV329_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8940816.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(7906..9471)
                     /gene="guaA"
                     /locus_tag="IV329_001640"
     CDS             complement(7906..9471)
                     /gene="guaA"
                     /locus_tag="IV329_001640"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8940817.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9526..10800)
                     /locus_tag="IV329_001641"
     CDS             complement(9526..10800)
                     /locus_tag="IV329_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8940818.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            11088..>11414
                     /locus_tag="IV329_001642"
     CDS             11088..>11414
                     /locus_tag="IV329_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8940819.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKAR"
BASE COUNT         3564 a         1926 c         2435 g         3489 t
ORIGIN      
        1 ctaaatagat taaacttact ggtgagtggt cttgaacata cttttaaatc acattttact
       61 gagttttatt ggattttata attcattgga aacctcgtac ttaatctgtt tttatctatc
      121 gactagaatc tgtatatatt ccagctatca atgaggccgc agaaaagggt gcttaaaaat
      181 aatctgttgt ttaaaatatt attatcagaa tacttagata caaaattctg gcacaaagta
      241 aaaaaaatat tgacttaaaa aaatggatat ggtaatctat aaacgaaaat agtggtaacg
      301 tttccaaaat gcatcttcgt taaattttta tttttttaaa cgaaaatgga aacgtttcta
      361 aaatgtattg ggaggagatt gaattggaaa agacattggg accaggtggt gagataaggg
      421 cggtacaacg ggataaaaaa aatatttcga agtgggaaaa attaaagaaa cagttgcggc
      481 gtgataagtg gatgtacttg ttgctcttac caggtatcat catgacgttt atctttaagt
      541 atgtgccaat gtacggtgct gtgattgcat ttaaagacta taatcctatg ctaggaattt
      601 tggaaagtcc ttgggtagga ctagatcact ttagggaatt tatcagttca ccaaatttta
      661 cgcttttgct gagcaacaca ttaaaaatta gtatttatgg tttattgtta ggtttttttc
      721 caccaattat tttagcactg agcttgaatc aaattatcaa taaaaaaata aaacagaaaa
      781 tccagttgat tctatatgcg ccgaatttta tctcagtagt tattattgtg gggatgctgt
      841 ttttgttcct atccgcaaat ggacctatta atgggttagt agaaaaggtt acggggcaac
      901 caattatgtt catgtctaat ccagcttatt tcagaacgat ttatatttta tcgggaattt
      961 ggcaaggaat cggctggtca tccattcttt acaccgcaac gctggcggga gtcagtaccg
     1021 atttatatga ggcagcaaat atcgatgggg caaatatttt acaaaagatt tggcatatcg
     1081 atttgcctgc attgaggcct gtgatggtga tcagcttcat cttggccgcc ggcggtatta
     1141 tgaatgtagg atatgaaaaa gcctacttga tgcaaacttc aatgaacatc cctgcctcag
     1201 aaatcatttc cacttatgta tacaaagtcg gcttgcaatc aggtgattat gcctactctg
     1261 ctgctgttgg tctgttcaat atgattatta atgtcgtttt attactgatt gtcaacaata
     1321 taaccaagcg gctaaacgat ggtcaaggct tgtaatgaaa ggagaaaatt atgaatagtg
     1381 ctttatcaaa aatcgaccgt caaatcttat ggttgaatcg agtgattgta acttttttag
     1441 tcgtcatcac cattgttccg ctgatttatg tactggtagc ttcttttatg gacccagcta
     1501 ctttgatcaa taaaggtata agcttcaatc cgaaagattg gacaatacag ggatatcaac
     1561 gggtatttgc agatgattcc attttacgcg gttttgtaaa ttcgttgttt tactctttta
     1621 gcttcagctt attgactgta tttattacga tgatcactgc gtatcctttg tctcgaccta
     1681 acttggcggg gaaaaagatt attatgactt tttttgtcat tacaatgttc gtgggtggtg
     1741 gcttgatacc tacctattta ttggtaaaga acttgggcat gttagatagt gtatgggcga
     1801 ttatcgtacc aggtgctata aatgtgtgga atattatctt agcccgtacc tattttagtg
     1861 ctttgccaaa agaactttct gaagctgcta ctatcgatgg cgccaatgac ttgcaaatac
     1921 tgttcaaagt catgcttcca ttagcgaaac caattatgtt cgtattgttt ttatatgcct
     1981 ttgttgggca atggaactca tactttgatg ccatgattta catcaaagat cccgacttac
     2041 agccgttgca actcgtatta agaaatattt tgatccaaaa ccaaccttca caagacatgg
     2101 tgggagccaa tactgccatg gcagaaatga aacaaattgc tgagttgatc aaatacgcaa
     2161 cgatcgtcat ttctagtctg ccattaatca taatgtatcc tttcttccaa aaatattttg
     2221 ataaaggtgt tttagttggt tctgttaaag ggtaagtatt gttcactatt tgtatgaata
     2281 aaaattataa aggagatttt aagatgaaag caaaatggat gctgccaatc gtttttactt
     2341 ccgtactaat tttgggggct tgtggaggct caaaagaaaa gagtaaatca agccctgatt
     2401 atgaactaga aaatgtctct tttccattga aagaaaaagt atctttaaaa atgatgtctc
     2461 aaagtgcccc tttggctccc aatgatccta atgacaaatt gattttccaa cgattagaag
     2521 aagagaccaa tctccatatt gattggacaa actacagtag tgattttgcc gaaaaaagaa
     2581 atttggatat cgctagtggt gatttaccag atgccatctt caacgccgct gcaggtgact
     2641 atgatttatt aaactgggcg gaaagtggcg ttattgtacc gattgaagat ttgatcgaag
     2701 attatatgcc aaatctgaag aaaatttttg atgaaaaccc cgaataccga caaatgtcta
     2761 ctgcacctga tggtcacatt tactctatgc cttggattga agaattaggc gaaggaaaag
     2821 agtcgattca tacagtaaat gggatggctt ggatcaacaa ggagtggttg gataatttgg
     2881 gtcttgaaat gccgcaaacc actgatcagt tgatgactgt gttggaggct ttcaagacgc
     2941 aagatccaaa tggtaatggt gaagcagatg aaattccttt tagctttatt aacaatggag
     3001 gaaacgaaga cttaaaattt ttaatggggg cattcggtct aggtgataat gatgaccact
     3061 tagtcgttga taacgatggt caggtacaat ttactgctaa caaagaaggg tataaagaag
     3121 ccatcaaata tttcaatgaa atgaataata aaggtcttat tgataaagag gctttcgaac
     3181 aagattggaa tacgtatgtt gccaaaggga aagaacataa gtatggcgtg tactttactt
     3241 gggataaagc aaatatcacg ggtgcagatg atagttatga cgtcttgcca gtcttagcgg
     3301 gaccagatgg aacgaaacat gttactcgta cgaatggcat gggtttctca agagatcgtt
     3361 tcgtaattac tagtgcgaat aaaaacttgg aacttacagc taggtggata gataaaatgt
     3421 atgaaccgtt acagtctgtc caaaataact ggggaaccta cggtgatgaa gcacaggcga
     3481 atatttttga attaaaggat ggtatgttaa aacatttgcc tttagagggg acagcaccgg
     3541 gggaactaag acaaaaaacc gaagctggag gaccattagc agttcttgat tcttattacg
     3601 gcacagtcac tactatgcca gatgatgcga agtggcgttt agatttgatg catgacacgt
     3661 atttgccata tattacagaa gacaatatct atccacgtgt ctttttagaa aaagaagaaa
     3721 cagatcgatt agcaaaaatc gaaacggata tgtgggatta catctatcgc aaaagagcag
     3781 agtggatcac cggcggtaaa atcgatgaag aatgggaaga ataccttgct gaattggaac
     3841 gtgtaggttt ggaagaatgg attcaaataa aacaaactgg ctatgataat tttttgaagg
     3901 gagcacaata agtgttagta gaagaaacgg cgattttgga taaagcaaat cgatttattg
     3961 aaatgatgcg aggaaaagtt aaccctcagt atcgacaaaa ctatcatttt tccgcaccca
     4021 tcgggtggat aaatgacccc aatgggttta tatattacaa agggcattac catctcttct
     4081 atcaatacta tccttacgat agtcgatggg ggcccatgca ttgggggcat gcgcgcagta
     4141 gtgatttggt ccattgggaa gatctaccag tagcgttagc cccagatcaa ccttatgaca
     4201 gcggcgggtg tttctcagga agtgcaattg aaagagacgg aaaactatat ttattttata
     4261 caggctgtca agaaattgat ggtattagcc atcagcatca atgtttggcg gtctctgaag
     4321 atggtattca ttttagcaaa tacagtggta atcctgtggt agcagaaaaa cagctaggtg
     4381 aaaatgggct aatacaagaa tttcgtgatc ctaaagtaat tgttcacaac gatggttact
     4441 atatggtagt agctactaaa tctacacagt ctactggtag aatagtgatg tttcaatcgt
     4501 cagatttgta tacttggcac ttttactctg tgctgctaga agggacttca gagcaaggaa
     4561 taatgtggga atgtcctgat ttattccatt tagacggacg agatgtctta atcatgtctc
     4621 cgatacaaat caagaagaac ggactggaat atcataatat cagttctacc atggcttgta
     4681 ttggtgaggt aaactgggaa acaggtcaga tgatagttga taatttccat gaaatggatt
     4741 ttggaatgga tttttatgca ccacaaacaa caagagatcc taatggtgga cgggtgatga
     4801 ttgcttggat gcagatgtgg gatcgtacat tgcctacaca tgagttaggc catctttggg
     4861 caggctctat gagtttgccc cgagagttaa gagtgaaaaa aaatcgattg gtacaaaaac
     4921 ccatctcaac tgtatatagt catttagttt atcaacaagg cagtgaaaat attgcagtga
     4981 ctgacacatc agtcgtattt cgaaacgcgc tgtctgattc ttcatattta catctagtcg
     5041 cagatttgga gaaagctgaa gtttttacaa ttgaattact gcggtctaag acggattgtc
     5101 tcaaactagc ctatgacact gctacacagg tttttgaatt ggatcgtcag aaactaggaa
     5161 gagagcttac aggggcggaa gaagaccctc tgaatgtacg aaaagttgaa gtacctttga
     5221 ttgataatca gctcgttctt gagattttta gagatacctc atcaattgaa gtctttatta
     5281 atgggttaga agttatgacg gctactttct atgagctgga aaaagctcga gatatcttgt
     5341 ttcattcgat cggagaaagt cgtattcaag cttttgaaac agcgagtgtg aaataaatgt
     5401 ttgattgctt aggcgatgaa tcaattattt aataggtttt gaaggataat gtttttagaa
     5461 aagaacagat agctagattt cccatggatt tgctattagt attttgaaaa aattagtaat
     5521 ttatgacaaa attcattata ctaaacttgt gcagaaatgt gagggatcaa ctatgaaagc
     5581 aacaatgaaa gatgtcgcaa aattagcagg tgttggagta ggaactgttt cccgggtatt
     5641 gaataacggt tctgttaaag cttctactag agaaaaagtt gaagcagcaa tgacagagtt
     5701 aaactatcaa ccagattttt atgcccgggg gttaaagatg aagcatacga acacggttgc
     5761 tttgattcta cccacggtat ggcatccatt ttttggcgaa tttgcctacc atgtagaaac
     5821 agcattagaa agacaaaact acaagcttta tttgtgtaac tccagtggta atagtgaaaa
     5881 agaacaagaa tatatccaga tggtcagcca aaataaagta gacggcatca ttggaattac
     5941 ttattcagat atcgatgaat acatttcgag taatctgcct tttgttagta ttgaccgtta
     6001 tttcactgaa gaggtgattt atgtgaccgc tgacaatgcg gccggtggac gtttggctgc
     6061 aaatgaactt tatcgccgag gatgtcagaa tgtagcatat atcggtgggt accaagaaac
     6121 accgaatgaa acgaagagtc gtcgaaaata ttttgaaatc gagtgccgtc aactggggat
     6181 ttcctataaa gcgttagata tgttagaacc tttagaccat ctctcagaac gtgtaacaaa
     6241 ttttttgtta gagaatcccg atatcgatgg gatttttacg atcaacgatc caatggcatt
     6301 agctgtcatt gatatgcttg aaaaaatagg gaaaaacgta ccagaggatg tccaagttat
     6361 cggatttgat ggtcaaaaga tgacagaagg agatccttat ctagtctcaa cgattgccca
     6421 acctacaaag caaatggcgg aagtggcagt ggcaaaatta attgctgtca ttaatggaga
     6481 gacagtagaa aaacgtaccg tattgccgat tgaatttggc gaaggtgaca caacgaaacc
     6541 tttagtgaga aacgattttg cttagtgcaa atacgtttct ttttttgaaa atgagtgaat
     6601 gaaaggtgta gataaaatgt tatatggtgg aatagaagcc ggtggaacaa agtttgtctg
     6661 tgggattggt aatgaacaat tacaagtgat agaacggaaa agttttccta ccacaacacc
     6721 agaagaaacg atggagcaag tgtttgattt ctttgataag tacaaaggaa atatcgcatc
     6781 aattggtgta ggagcatttg ggcctattga tattcaaatc gaatcaagaa cttacggata
     6841 tatcaccaac acaccgaagt tagcttggca aaattatcat tttgtaggag ctttggaaaa
     6901 cgccttgtcg gttcccatct attggactac cgatgtaaat gctgcttgtt atggagaata
     6961 tatggcaggg tatggaaaag gtaaaaatag tgttctgtat tacaccgttg gtacaggtgt
     7021 tggtggtggt gctgtagtgg atgggaaaat agtcgcaggt tttagtcatc ctgaaatggg
     7081 acatatgctc ttacaacgtc ataatgaaga tcaatttgaa ggaatctgtc cttttcatca
     7141 tgattgtttg gaagggttag catcaggacc tgcgattgaa aaacgctttg gaagaaaagc
     7201 ccagactatc tcatttgatc atccttattg gacgattgag gcagattatc ttgctcagtg
     7261 tgtgtataac acaacgttac tatatgcacc ggaagtaata attttaggcg ggggtgtaat
     7321 gaaacagccg caactactcg agttgattcg ccaatcgttt aaagaaaaaa tgggttccta
     7381 cgtggatacg cctactctcg aagaatatct actaactcca aggcttgctg acaatgcggg
     7441 cttgattggc tgcctagctt tagcaataaa gaaactcaat cattagaata gtatttatta
     7501 gaactattca taacataccg gaagtttaaa agggtattct gaaacttaaa tagtctcaat
     7561 tcattcttct ttcttgagaa tctgagtaga gacttttatt ctgtttcaaa ttaatataat
     7621 tacgaaatga tattgagtac aagaaagttc tttttaccag atcttttaaa tcgaaaaatc
     7681 tgatatatat atttatagta ctaaaaatct gattagataa aaagctaatt caataggaaa
     7741 ataaaggtag tgaataaaag caaagccctt gttagtagtt aatctaacaa gggctttggt
     7801 agggaaaatg gtaggaaacc atcttatttt ttacgaaaat caggaacttt agtaattgaa
     7861 taatgttgat ttataggtat tcctcatcca tattatgttg gctctttatt cccactccac
     7921 tgttgcaggt ggtttgctgg taatatcata cacgatacgg ttaacgtgtg cgacttcatt
     7981 tacgatacgg acactgattt tttgcaagac ttcccaaggg atacgggcaa agtcagcggt
     8041 catgccgtca atagaagtga cagcacggat acctacagtg tagtcatacg tacggccatc
     8101 tcccataacc ccaacagagc gaatgcctgg taggacggtg aagtattgcc agatgtcgcg
     8161 atctaaaccg gcattggcga tttcttcgcg caagatagcg tctgattcac ggacgatttc
     8221 taatttttct tctgtcaatt caccaaggac acgaatacct aagcctggtc cagggaatgg
     8281 ttggcgccaa acgatgctgt caggcatgcc taattgggta cctaattcac gtacttcgtc
     8341 tttgaacaat gtgttcaatg gctcgatcag cttgaattgc atgtcttcag gtaatccacc
     8401 tacattgtga tgtgatttga tggtttgggc agtttcagtt cctgattcga tcacatcggt
     8461 ataaagggtt ccttgtgcaa ggaaagaaac gccttcttca ccggcaagtt tggttgcttc
     8521 atcatcgaag acatatacaa attcatttcc aatgatttta cgtttttctt ctggatcaga
     8581 cacgccagca agttttgata agaaacgttc tttggcatcg acttttataa tgttcaagcc
     8641 aaattttccg ccaagacttt ccataacttg ttctgcttca cctttacgca acaatacgtg
     8701 atcaacgaag atacatgtta attgatcgcc gatcgctttt tgtaaaagaa caccaacaac
     8761 agaggaatct actccgcctg aaaggcctaa taaaactttt ttgtcaccga cttgttcacg
     8821 aatttttgcg atctccatgt cgatgaagtt atccattgtc cagtcaccag tacattggca
     8881 gacatctagt gtaaaatggc gcaacaagtc atttccatat tcggaatggc gaacttctgc
     8941 atggaattgg attccgtaca ttttgcgttc tgcattttgg atcgatgcga tggggcaatc
     9001 tttgctggta gcaacgacat cgaaatcagt cggtgcttgg gtaactaggt caccatgact
     9061 catccaaacg gtttgttttt caggagtgtt ttggaaaagg gccgcttctg cagaagtgat
     9121 ttccaattct gcttttccgt attcacggtt tttggctggt ttaacttttc cgcctaattt
     9181 ataagtaatc aactgcatac cgtaacagat tcctaaaact gggatcccca aatcaaaaat
     9241 cttttcatca ataccaaagg ccttctcatc ataaacactg tttggtccgc cagaaaggat
     9301 gattccttta gggttcattg cctttacttc ttctgcggta atgcggtggc tcatcaactc
     9361 tgagaaaaca ccgaactcac gaatacggcg agtgatcagt tggttatact gactaccgta
     9421 atcaagtacg ataatttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     9481 aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     9541 cagaccttgt tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc
     9601 aattcgttta atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtggga
     9661 caccttaaag gcatttgaaa tacccgattg gtagcgatgg aagatcgtga atttcttttt
     9721 aaacgactga ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc
     9781 tcgtttaacg aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc
     9841 gttgtagctt aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg
     9901 atgataatgc cgtttgagtg gctccaatgt cgtggaatct ttgagtaaga gtttccaata
     9961 tcgcttgagc cgacggtact tcttctgatt ttcagagtgt gaggttcgaa aacgattcat
    10021 gatctggaca cgtaattgat tgaagctgcg attgatgtgt tggacaatat gaaagcggtc
    10081 agttacgatc tccgcatttg gaaagactgt tttaagcaac tgacaatagc tggcattcat
    10141 gtccatcact aggtacttga cctttaaacg ggcttttcta ggaaagcgca ggaagtattg
    10201 agttagtttg taaagtctgc gatcttcaag gatcgtaaat agctgatggg ttgtcccatc
    10261 aacacaaata aaactcatgg aaccagaaac agacttcatt gatttgaact catccataca
    10321 caacaccgtc ggaaggaagt tcatacgtgg ctggtaatgc ttcgttgctt cacgcaagat
    10381 ccgtgtcacc gtcccatccg agacgaagta cttcttagcg attagtttac gtgattgatt
    10441 ttcgtacagt tctatgagaa tcgcctgctt cagttccttt gttagttgat gatgttcatc
    10501 aactaagtcc gttttcgcag aaaaggtctg cccacaatca tagcatagaa aacgggtacg
    10561 ttttaattct aaaatagtca acttttcttt gactttcaat atctgagtac gtgtcgtata
    10621 ggaaccgttg cggataatac gaatcgatcc acagtgttca caatgggaag gtgagtcatc
    10681 caatctccct ttaataaact gcgcttgaac gcctttatat tctttttcaa tcagccattc
    10741 ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat tttctagtat gattatccat
    10801 agagcgactc ctttggtagg gtgatgtttt ggtcgactaa attttaccaa aagtgagtct
    10861 ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat cgccttcgat tttccaacaa
    10921 cacaaaatat tatagaaccc tttattctaa aggatttcaa ggtgtctgtt tgcatttatt
    10981 agaaaaaaga aaaagtattg gcaacagaag gtgtcaccaa cactagatat tatagagcca
    11041 taatatgctg tataatatct agtgtatagg cttaagtgga ggtatatatg aaaatattaa
    11101 ctgttgaaga tgataatatg ataagagagg gaataagtga atatctttca gaatttggat
    11161 atactgttat tcaagctaaa gatggaagag aagccttgtc aaaatttaat agcgatataa
    11221 atttggttat cttagacatt cagatacctt ttataaatgg tttagaagtg ttgaaagaaa
    11281 ttagaaagaa aagcaattta ccaattctaa tcttgactgc atttagtgat gaagaatata
    11341 aaattgatgc atttactaat ttagtagacg gatatgtaga aaagccattt tcattgccgg
    11401 tcttaaaagc taga
//
LOCUS       DACDKL010000081        11286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDKL010000081 DACDKL010000000
VERSION     DACDKL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.081"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..1479)
                     /locus_tag="IV329_001643"
     CDS             complement(16..1479)
                     /locus_tag="IV329_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8940820.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1543..4560)
                     /locus_tag="IV329_001644"
     CDS             complement(1543..4560)
                     /locus_tag="IV329_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8940821.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4711..5550)
                     /locus_tag="IV329_001645"
     CDS             complement(4711..5550)
                     /locus_tag="IV329_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8940822.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5695..5940
                     /locus_tag="IV329_001646"
     CDS             5695..5940
                     /locus_tag="IV329_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940823.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6003..6425
                     /locus_tag="IV329_001647"
     CDS             6003..6425
                     /locus_tag="IV329_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940824.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6519..6836)
                     /locus_tag="IV329_001648"
     CDS             complement(6519..6836)
                     /locus_tag="IV329_001648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940825.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6863..7069)
                     /locus_tag="IV329_001649"
     CDS             complement(6863..7069)
                     /locus_tag="IV329_001649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940826.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7050..7232)
                     /locus_tag="IV329_001650"
     CDS             complement(7050..7232)
                     /locus_tag="IV329_001650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940827.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7246..7596)
                     /locus_tag="IV329_001651"
     CDS             complement(7246..7596)
                     /locus_tag="IV329_001651"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940828.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7622..7924)
                     /locus_tag="IV329_001652"
     CDS             complement(7622..7924)
                     /locus_tag="IV329_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940829.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(7927..8160)
                     /locus_tag="IV329_001653"
     CDS             complement(7927..8160)
                     /locus_tag="IV329_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940830.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9146..9468)
                     /locus_tag="IV329_001654"
                     /pseudo
     CDS             complement(9146..9468)
                     /locus_tag="IV329_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9591..10706)
                     /locus_tag="IV329_001655"
     CDS             complement(9591..10706)
                     /locus_tag="IV329_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940831.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10712..11110)
                     /locus_tag="IV329_001656"
     CDS             complement(10712..11110)
                     /locus_tag="IV329_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940832.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         2955 a         2330 c         1745 g         4256 t
ORIGIN      
        1 aaacagcctt attctttaga ataagactgt ttctgggttg attaatttgt cttttgttaa
       61 actaccatct aaggcaagtt ctaaatgtaa atgtggtcct gtactatttc ctgttgaacc
      121 cacataacca ataatttgtc cttgttcaac tgtttgtcct acgttcacgc aatattcctg
      181 ttggtgcgca taaagagcca cacttccgtc ttcatgctca attgtgacat aatttcctga
      241 caaagtatga tacgaagctt ctatgaccgt tcctgccttg ctcgcataga ttggttctcc
      301 ttgattggct gccatgtcta atcctctatg aaattcttct cctcgccaac cgaaggaact
      361 agaaattgaa taatttttta gaggtaccat atagccttct ttgcttagtt gtggaccagc
      421 tacttcttta tcatatttga ttaagtcata ggtttcgatt aacccattta attttttatc
      481 gtagctagtg tctgttgcgt atcttcctgt taaaaattta gtcgcttcct gataggtttt
      541 tgccttactt ttccaaacac catcataaaa agaggtattt ccaactaagc cttcttttaa
      601 cagtttggca tagtctttta agctttcttc atagctctca tattgccgaa aagttgcttg
      661 ggtcgtataa agtgtcccat tgcctaaatc ttcttgtgtc gcaaaagaga cgccctttcc
      721 gttatgtgtg cctttgattc caaatagatt gtagtttggt gcctgagcca attgactttg
      781 tccgcttgcg gattctaaaa tggcttgcgc gatcataaca gaagcgtata aatcattttc
      841 ttgtccaatt ttacgagcag attcaccgat tttacggata aacgtttcta cagacgcgtc
      901 tttttcaaaa tgaatagtcc cttcgttatc ttgtgtgctg gaagggacta attcttgtat
      961 cggtgcgctt ggtgcttggt ggtagttttt tgtctcttct gaaccgattg attgtgcctg
     1021 cggtacgtca ttcataggta cagtcacact ctgttcaggt gccttttgtg ggcgttccgt
     1081 ttttgttggc ggttccgctt ctgtttcggt acttggtttt tgaggagtct gtgagctttc
     1141 atcagaaggt tgctcatttg gttcttctga cggttcctcc tcaggttgtt gattgtcttc
     1201 tggtttgtct ggttgattcg gtgtttctgg ttgttcttca gaacctgttg gtggagcgtt
     1261 catttctgga ttatctggtg tatctattgt atcttcagtc ggtgtttctg gttgattttc
     1321 ttctccagtg gaatttgttg tcgtttcttc agtgatcgtt gcagattgcg aatttgtcag
     1381 ttgattgttg ccttgttcat tagccaaaac cgctggcgta tacccaaata cttgacctac
     1441 taataacaaa gcagataaac atttactcgt ttttttcaca agattgcctc cattcttcaa
     1501 tcaatcatct agagacagct gattgctgtc tctagtcata ctttaattgc tttgtttcat
     1561 tttacgacga accagaacag cccccacacc aacaaaaaga aacagtccca atgcaattaa
     1621 cacatcgttc actgtttctc ctgtttttgg taagctttgt tttggtgtgc tcgtcggcgt
     1681 ttgtttgaca ggggttgtcg gcgtttcttt tttaatgcct tttgctgtca atgtttgttt
     1741 ttcattcttc aaatcaaagt gacgtgcttc ttcctcttta tggtctttat ccttatagac
     1801 aatatgagtt gcgaccaatt tctccccgtc ttttaacgtg cctgctttgt agtcaaagga
     1861 tactttaaac tcttcagacg ctttagaaac tttacgtgca gtttctaccg taccaacgac
     1921 tgtatctttt ccatttttat caatcttatg gaattgagtc acccagtatt tggtttcccc
     1981 taccgtagat tctggaaatt cttgcgatac atgatcgatc aactcttggt ctttgtctgg
     2041 atcaaatgtt tggtcgccat tcactgtaat aaagcgtgtt tcgatttttg gttctttcgc
     2101 agtcactgtt tgatctttat tggttaaatc aaaatgacga gcgaattctt cttcctcttc
     2161 tttgtcgtta tagacaatgt gcgttgcgac aatcgtgtca ccgtctgaaa ccatgtctgc
     2221 tttgtagttg aacgtcacat caaatgtttc tttttcatcg gtcacttttc gtgtttcttt
     2281 taccgtatct aatacagtga cttttccatc ggcagtaatt ttatggaatt gcgtcaccca
     2341 atatttctct gtcccaaccg cagatttagg gaatacttgg gacactttat ccactaattc
     2401 ttggtctttt ttcgggttga aatcttttcc acctgtcgtt gtcacaaaca aagtagaaat
     2461 ttctggttgg tcttcttctg gaaccacgat ttcttcgttt ttcacagtaa ttggttgtac
     2521 ttctccgtct ttgaccgtta ctaaaatcgt ttctgtttgt cctgacgctt gcccataatg
     2581 ctcagggaac acaatttccg taaatttata cgtaccgtct ggtaatttta gtttttctgg
     2641 ttcttctttt gtttcataca caaaggacac atttttatta tgtgcagtca taccttcaat
     2701 caggaatttc gctcctggta attgtagatg attgattgaa tcgactttat tgaagtctac
     2761 ttcacccgtt tttaacaagt tgtcaatcac atctaattca aacgtaagca caggcgtcgt
     2821 gttatcgcca taggccacag agaacgtggt ttgttcttca gaaggttgac ggtagttgtc
     2881 tggcacattt ttttcaatca attggtattt gttttgatcg ccattgtaga attttttgtt
     2941 tgccttccag tgtccgtctt tgtcagtttt tactgaatcc aagacttctc cttttttcac
     3001 aaggactgtt tctttatctg gcgcagtaat atctgttttg gcgataatat caaattcgat
     3061 attttctgca ggaacggtat cataaacagg tttgccttga ttccaggatt cttccgcatt
     3121 atagacttct ttgactttct ttccttctgg aatgaactgt tgtaagtcat tttttgcagt
     3181 taacgtcact tctttgtttg gttcaatcgt aatcgtatga gtttgtttgt ctagaatata
     3241 cccagctggt gctttgactt ctttgacatc caatttattt tgaacatatt ttccgtcttt
     3301 ttcatagaat gcagggattt cttttaaggc gatttcccct ttggcatccg ttgtatattg
     3361 ttttgtttct cccgtatcat gacgtttgat ttcatacgtc acatttggaa cttttttccc
     3421 tgtgacagcg tctagtttgg ttagtttgaa gtcgccattt tttagaactt taatagttag
     3481 tccagctcta ttaggatctg gcacataaag ctttcctaga ttttgagcaa caggatctac
     3541 ataggcaaaa cttgaaccta cccctctaga atttggatcc agtgaagaaa cagaaattcg
     3601 tccgtcattt gagttttgat ttgcttttat aaacaaacta tttcctgatt ttcttacttg
     3661 aacgttagct gtgttttcaa caaccgaccc atcccattga gaaaacacac cttttgtatc
     3721 cttaacttct acttcttccc ctactttaac cgtcactgtt tggctatcaa aagaaggatc
     3781 ttcaagtaaa ttgtttattt ttctatctac agtctgtttg aaagcttcat aattagattg
     3841 tccaatagtt ccataattaa caaatgaacc acctaaaact tcccaaatat atgtttgtgc
     3901 taatacatag ttatccattg aaccactaga atagtagcca aaataaccaa ttaaagaaat
     3961 cttttttcta atagcacttg tcgtaattaa attttcgagt ggtgtcacat catattgaat
     4021 acccgcactc gtgattgcgc cttcttgaat gcaaaaaaca tcttctccgt caatttgcca
     4081 gcgaaccatg taatcatgag tatacattgt tccttcagga gtaattcgag tcatattcac
     4141 atctttacga ccattttcag tcaatgttag attggtagca cgtgtctgtg cattcttttc
     4201 caagattgct tgccattctt cagattcttg cgccaatcgt tcttgttcag aaaaagaagt
     4261 cgctttttga atatcttcaa tggttataaa gtatttctca taaccttcag ggacctcttg
     4321 agtcattaca ttgggcggag aaacatcttc tgtcgtgtcc tgcgtagttg tctcttctgt
     4381 tgtttcactt gtggtttcag tactgatcgc ttcttcacta ctttttgatt cttcagtctt
     4441 cattacttct tcagtagaat ttgattcatt tatcgtttct gcaaatgccg ttacagatcc
     4501 taacatggga ttgagaaatg taacgaaaat tgccaaccaa gttgccattc ttttaaacat
     4561 atttttttcg ttcctttcat tttatttttc agtcttctaa agtaatttct tatggacttc
     4621 gacaccgaaa catttttatc ctcctcaaaa caaaaaaaga ctagaactta taattctagt
     4681 cttcatcaac cacttacttt tcttttattt ttagtagaaa tctacagtgt gtttttgtga
     4741 cccgtcactg taaataataa catccaccat aaaacctttg ttgtaccact gcgaatttgg
     4801 atcgctaacc tctgcattag cccaatcata ggcttcatct acagtgttaa atacctttcc
     4861 actattacca atctctccaa ctacaattgt tggctcttca ggtgtcgttg tgccaccatt
     4921 tccgccttga tcgcctccac caggattgct tggttcttca ggcgttgttg ttcctccacc
     4981 tgtgcttggg ttagaaggag ttgtttctcc tccagtattt ccaccattac ctggttgtgt
     5041 aggtgtttga gtagtgccac cattgttttc tggtgtactt ggcgttgttg taccaccagt
     5101 tgttcctgtt tgatctccag ctggtgttgt tggtgtcgtt gtatttggtg tcgtttgtgt
     5161 atttgaagtc gttccttgtc ctactggttg attcggatca actttgtctt tctcttgaat
     5221 cactgtttga gccacagttt cacctgcgct gttttgtaca cttgctacat tgccattaaa
     5281 tactaattta tttcctgcat agattaaatc cacatttgta atcttattca tgtccgctaa
     5341 tttttggaca gtaatatttg ttgcttgact gattgcgctt agggtatctc cccagacaat
     5401 tgtgtattca ttccctttaa tttctgcttt gatttgttcc gccgaacgag gtgtccaaac
     5461 tttgttttct gctgcttcag cattcgttcc cacagcaaga ctaactcctc cgactacgat
     5521 tcctagtgcc aatactttca acattttcat tgtttttcgc ctccgatttg aataaattta
     5581 tttacaaaaa catagtacca aacttatgtc tatcagtcga tatatttttt gtagattttt
     5641 ttattttttg atatactaac tttggtacta aaataactaa aaggaatgtc acacatgcaa
     5701 cttaataatt taaatgaaat acatgattgg gctcaaaata atttacttaa tcgtaaacaa
     5761 gctgccaaaa tcactggaca aaactatgat gctttttcta gctctttacg tagaaataat
     5821 atcaaaccat ttctagaaat aggcgacgaa cctggtcctt ccatcattcg cttatatcta
     5881 agagaagata ttgaaaaata cgcaaaacag ctcgaagaaa aaagacagaa acgtcaatga
     5941 ggcgttttct gtcttggatt tacaagtaaa acaataataa acacttttac aacggaggtt
     6001 ctatgaataa gaaatacatt gtttcagttg gttcaatcgt actgattgta ctcgctatta
     6061 ttgctttttc tgtatccaac aaaacagaaa cacagccaca aaatacggtt caatcgacta
     6121 ctacaagtca atctagcgat cattccgtct atccttcact aaatagttta aaagaaacgc
     6181 atggacaaga tttaattgat cttggttctt ctcaaattac tactgtctta tttgaaaaga
     6241 aaaatattgc gtttattact cctaaagaag gaactgacct tgaattttct atcaaagtaa
     6301 caaataccaa agaaaattta ttctccattc ctgacattca atatacccct aacaagttaa
     6361 agattgggga tacgtttggt ttggctaaag taaatgaaca atactttgct tactcactga
     6421 aataagtttc taatagcaag aggaaaaacc ctcttgttat ttttatcaca taatcatttt
     6481 ataaaaacgc ttcgaaaata tcaacatttt tgaagaaact atttttctaa tttctcaatt
     6541 tcttccaagg gattttcgct aatgacccat acaggcacag attgctctcg aggctcccct
     6601 tcttctaacc accaacctgc ataatgaact tcttcctcct cagcattacc atgataccaa
     6661 ccaacaaagg attcatctac aacgaatgaa atttcttctt ctggatcaaa taattcattt
     6721 ttccatgctt caattgcttg ttctttggtg tacttttgtt tgttaaatac accacaaatt
     6781 tcgtcatcta cataaaaaat ttcaaaatca aaattactcg ttccttttac tggcatctta
     6841 acttttcctc ctttgactca cttcaataga tataccactc atgttcattc cattgtttac
     6901 tagttaattg gagttttatc ttagggcttt gtgcactctt tccaaaaact tgagcgtcgt
     6961 ttttttcttc taaccaaaaa aggtaatttc ttacattcgg tactaaatgt ttttcctgca
     7021 tttcttcatg aaaaacatat aattctgctt catttttcaa ccaattcatt attcattccc
     7081 tcattcactt cttcataccg ccattcagat agtgagattt ctttttgttt ctccacttgt
     7141 cctataaaaa tcgctgtatt caacaagctg atcacactta ataataaagc aatgaaacta
     7201 atggttacac ttactctttc acattttgtc attttgtttc cttctttact cgtttgttat
     7261 ttcttgtaat aggtctaata actccttcac cgaaatttgc aaatggttcg caaaaatttc
     7321 aataaaagca ctcgcacaca ttaccagacc ttcaactgat atatcactgc gacaataaaa
     7381 ttctggagaa acactgattt ctgattcttt cttacaattg aacgaaaaaa ctatttttga
     7441 atcaaattcc ggtaattctt ccacaactat gttttgggat tctaccaaga aagggagtgc
     7501 ctgttctaat gcttcttctg gtgtttgagc agatagaata attgcttctt gttcttttat
     7561 ttcattgttc tcaaatactt tataactttt tttcattgtt agttcctctt tcattcctct
     7621 ctcaattagc taagccgtta ctggtacgcc attctctaaa tttagtatcg ctaatcctta
     7681 atgctcgtcg aatatcaatc attcgataac ctattgcacg aagttcttta tatcttttag
     7741 cagttagcgt ctctaacgtc atttcaggca atgggttcga atcaaaaata gaattcgaac
     7801 cgattatttg ggccaattgt tgtacttgta acagctcttc tgaacttaat tcagtgattg
     7861 ctcgattgcc tatcattgca tacattttct gtcgaatata cttcttttct tgtttagaca
     7921 acatttctat ccactcctat tttcattatc atttgttacc tccacttcag taaatccaca
     7981 aataaatgaa ttcatattcc aagtaccatt cccttcaact acaacttttc tcctgtcctc
     8041 tttcggatat tcaagaatca gaccgtttac caatatgttt ttcactacta ctaatttgtt
     8101 tgtataccgt ggaattgctg ttccgatata cttcacttta tttcctacgc taattgtcat
     8161 ttcctcactc ctattctttt ttatccaaaa ctttttcctg tcctattcct gccaacgaat
     8221 atacttcact gcatgcttaa acaaaacaaa atgttgccct gttttatttc cgtcttttac
     8281 ttgttccaca tacagttcat atttggtcac accttttaaa acgccagata ctttttttca
     8341 tccattgtca caattagttt tttcatcaat cgattgatac tgcactgctc atttttcttg
     8401 ttcatatagt attcctttct ttttcaaaac tagcctcttt tgtttactag ttttcatcaa
     8461 tcatcaacga tttaacagat tttttctttc gcagggtttc tagcgtcagc aaccccaaac
     8521 tctaaaaaag tgcctttgct cccttgaaaa acgagcggac gattgtactc gtcatcatat
     8581 gtttttcata ctacctgatc cacgcaaata accttttttt gttctgtgcc acacccatcc
     8641 atacgtatcg cttgagattc accatacttt tttaggtggt ttatccttcc taagtccctg
     8701 ttagacacgc tgattaactt agaaagtcct gtttgtatcc aattttcaaa gatctcccaa
     8761 aagaaataag taggtttacg tgtatcgctt ctgttcaaag tacctgtttg tacttgagta
     8821 tttctactcc cctcgttcaa gggattcctt ccaccagcta gcttttcctt tcatcatcaa
     8881 atgttcctga aaagcattta cgtttcgtgt ccgtgtgctt acttattctt ttttcgatag
     8941 cgattatccg ctcgctactc gctattttat actttgtttg ttagctcaaa taaaaactaa
     9001 caaacaaaaa aggctagttt ctttccgttt tattttttta actgtcgata aatagttgtt
     9061 agtttaattc caagttctaa aaccaatacg cccaacaaaa ccacaggaaa aacaattggg
     9121 gcaaacaagt aaaatcggat agttttcatg tcttccctct ttctttttgg tgtcgctgtg
     9181 gtcgttcttg ccgttcaata cttcgataca ttttttattc attatttgct cgttttgttt
     9241 tcctctggac tgcttttctt gctttttgaa tactttatac gcttttcgtg cctgaattaa
     9301 taggtcttgt cggcgtttca ttcgtgagaa aacaatattt gaaaccatca aatcctggtt
     9361 cgaacaacca cgattcgcaa taattttccc atcaaaaaaa atagttttca cttgagaaaa
     9421 aaacagttgg aaatcttgtt ttgttgctaa tttttttgtc aaaatcactg tgcttttccc
     9481 tgttgaatct acaatgattc ttcgaacaat ccgattcgat aacttccaac gttgccgatt
     9541 aaacaatgtt acttcacaac aaatatgacg taagtgatta ggggtcattt tcatgtgtcc
     9601 aattcctcca accaattatg catcgaaatt ttgggcaatt gtgacagccc ataaatctgc
     9661 gtttccaggt cttcataact acaagaacgc ttggcttgaa gtaatgaata ttctgcttga
     9721 tctgatagtt ttgttgccat tggatctaga ttcaattctc ttacacaaca ttcccaaaaa
     9781 ttcaatagcg ctctaaaata aaatcctttt cgattctgaa ttgcttttcc ttcatgacga
     9841 gcaatttttt cattttgaat acaccgttga tacgttttgc ttaaatcttc tgcaaatgtt
     9901 tcccctaaaa ttcgatactc gcttccatca gataaaccat gtgtttgatt atactttcgt
     9961 tgccgctttt ctataattcg tttactgtcg tatatcatgt ttagctgctc tttcgcttca
    10021 cgccattcag ctgaactgcg aacaatccat gtcaaaacat ctttcggaac aaacgtactt
    10081 tcgctgtaat gagcaataaa ctctttttgc caatcatcag ccatttcaac aggactagtc
    10141 gtatcgattt ttgtatctag attttctgta tctagattgt tagaagaatt attaaataat
    10201 tgtgcagaat tttcacatgg atttttttcg tcaaaatcat cgaaaatcgc taaattatca
    10261 acagattcct cctcttcttt tccttgtgca gaatctacac tggttgagtc aacaggacct
    10321 aatgaataca catcattttc tgtcgtatct aattctccta gatacaaccg agctttgttt
    10381 ctttttcctg tttgttgatc aaatcccata tcctctattt ctagtagctc atattctttc
    10441 aattcttttt tgattgtatt aacagaacgt ctgcaacaat tcaatgcatt ggctaattct
    10501 tgttccgtaa attcaaaata aacaaaacct tgactatcta cttgatttcg ttgaatggct
    10561 aaatctaagc gtgaccgaaa aaccatatac gcgatttttg ctttatcaga caatgagcga
    10621 tatttttcac ttgtaataaa tacctttgga aattgatagt ataattcatt gtatttacga
    10681 gttgcggtaa atctttttgt tgccatcgtt attacacctc atcttgtcca tatattaaga
    10741 aagaattttg gtgttggtag acacgaacat cttccattgt agcaatcaat tcttttagat
    10801 aaattcgacc gaattcaatt gttagtaagt cgtaatcttc taattcattt ttgatttctt
    10861 caatttcttc ttcagacaca ttgagtaaaa tggctaattc ttgattggta taagaaatat
    10921 agacatgttt cttttcatca atccgttttt caattaagga ttcttctaag tactcattca
    10981 gtaagctata ggcgtatttt gcttgtgcag tcatcgcata atattttgac gattttataa
    11041 agaatagttt tggcatatga aaagttgctt gtgtcagcac atctgttgcg cgaaatttgt
    11101 tttttaccat attttttcct cctagaaaaa cacgcaaaaa aagctctgta taaatcgaat
    11161 tacacagagt ttcttcttgt tgatatttaa tttctaaaat acgtttcggg taaaaactaa
    11221 acacttacta atatttagat tgtagaatct tcgccttcat ttattcattt taaagagaaa
    11281 aagatc
//
LOCUS       DACDKL010000082        11183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDKL010000082 DACDKL010000000
VERSION     DACDKL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.082"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..303
                     /locus_tag="IV329_001657"
     CDS             4..303
                     /locus_tag="IV329_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8940833.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            376..1737
                     /locus_tag="IV329_001658"
     CDS             376..1737
                     /locus_tag="IV329_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8940834.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1759..2019
                     /locus_tag="IV329_001659"
     CDS             1759..2019
                     /locus_tag="IV329_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940835.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2033..2785
                     /locus_tag="IV329_001660"
     CDS             2033..2785
                     /locus_tag="IV329_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8940836.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2879..3484)
                     /locus_tag="IV329_001661"
     CDS             complement(2879..3484)
                     /locus_tag="IV329_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8940837.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3537..4511)
                     /locus_tag="IV329_001662"
     CDS             complement(3537..4511)
                     /locus_tag="IV329_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8940838.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4889..5668)
                     /locus_tag="IV329_001663"
     CDS             complement(4889..5668)
                     /locus_tag="IV329_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8940839.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5735..7006)
                     /gene="celB"
                     /locus_tag="IV329_001664"
     CDS             complement(5735..7006)
                     /gene="celB"
                     /locus_tag="IV329_001664"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8940840.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7159..8556
                     /locus_tag="IV329_001665"
     CDS             7159..8556
                     /locus_tag="IV329_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8940841.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8637..9077
                     /locus_tag="IV329_001666"
     CDS             8637..9077
                     /locus_tag="IV329_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8940842.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9225..9551
                     /locus_tag="IV329_001667"
     CDS             9225..9551
                     /locus_tag="IV329_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8940843.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9552..10301
                     /locus_tag="IV329_001668"
     CDS             9552..10301
                     /locus_tag="IV329_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940844.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10381..10836)
                     /locus_tag="IV329_001669"
     CDS             complement(10381..10836)
                     /locus_tag="IV329_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8940845.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3549 a         1958 c         2295 g         3381 t
ORIGIN      
        1 aaaatgaaaa atcggttaat tgtcgcatgt ggaagtggag tagcaacgag tcagacaatc
       61 gctagtaaga tccaaagtat gttagaagat gacggcatcg catttcctgt agaagcagtt
      121 gattacaaat cgattcaaaa tgagcttcta actgctggaa tctatgtgta tgtcgcacaa
      181 cctgatgagg aagtacttga acaagcaaaa gatttgggta ttgaagtatt tccaggaatt
      241 ccatttttaa ctggaatggg tgtagaacct atttatgaca gtatcaaaga gttagtacag
      301 taaaggagga ttgttggata aaaaagttgg ttcatagttt tatttactat atttagaaag
      361 aaagaaggtt tatttatgga agttttccaa accattatca attatgtttt gaacttagga
      421 tcagccattt ttgttcccct gattattttg atacttggat tgttggcagg gatgaaatta
      481 aaaaaagcat tcatgtctgc tttgacttta gggattgctt ttagcggaat gtctatggtc
      541 attggctata tgtctaacgc agtaagtcca gcatcagaag cattggctaa aaatacggga
      601 atcagtttgc ctgcacttga tcttgggtgg acaggtgcag caagcatcac atggtcgtgg
      661 agttatgctt ttatattttt tgcagtaacg attggggtca attttgtact gttacttttt
      721 aattggacga agacgttaaa tgttgatatg tggaatgttt gggggaaagc tttaactgct
      781 tatttagttt attatgtttc cggtagtttg gctgcaggct ttcttactgc aatggtccaa
      841 gttatattgg aattaaagct tggtgatatg tttcaaaaac atatacaaga tctcacaggg
      901 attccattag taacagtgac tcattttatg acatctgccg ctgtcttgtt gctgcctttc
      961 aatatgatca tggataaaat tcctgcttta aataaacgag cggatacgaa tgcattgaag
     1021 aagaaaattg ggatcttctc tgaaaactct gtaatgggat tcattattgg attggggctt
     1081 ggctttgctg ctgcgtatgg cgtttcaggt tctcttaatc tagcgatcca aacagctaca
     1141 gcattaagtt tgtttcctat gatctctaaa atgttcatgc aatctttgtc tcctctagca
     1201 gacgctatgt cagaatttat gaaaaagcgg tttaaggatc gtgaagtata tatcggattg
     1261 gactggccga tattagctgg tcgttcagaa atttgggtta ctgctatttt acttgttcct
     1321 gtgtttattg gttatgccat tattttgcca ggaaaccaag tattgccatt agcagggatt
     1381 atcaactatt cgattgcggt cggcggatta cttttaacag gaggaaactt ggctcgtatg
     1441 ctgatcttgg gaatcatcac tatgcctatt tatttgtacg gggccactta ccttgcgcca
     1501 atactcagtg atttagcaga aaaaagcgga gcagtcgagg gaatcaagaa aggacagttg
     1561 atcacatggt catccataga agggccggag ttcagggttt tatttgcgga ggcatttaat
     1621 aggaatattc tcgctattgt aggatgcgtg atttttctag gactgtttgt tctactttat
     1681 aaatatatga taaaagcgaa agtaccgagt gaacgttatg ctgaactaca aaaatagcat
     1741 tgaaatggtg ggaaagtaat gaaaagagaa gagacagata aaataaaatg gacagtcgct
     1801 ctttgtggta cacttctgct atttctatat ggactcttta ctcagaatat catcatcaat
     1861 ctattggtca tttttttcgc actagtgatc tataaatatg gaaatcacgt tttattcaga
     1921 gagtacgatg aaaaaagaaa acggaaaata gaagaatcaa tgaaaataaa agaagctacc
     1981 aaagaaatat tgagagaaaa aagttttata aaaagataat ggaggggtca ttatgtcaga
     2041 tggacgtatg ttatttgaga gagaacgaca ggacatggca cgtatcgtaa gagtgatttt
     2101 cgaaaggaaa aacacaaatg tcgcaggagg gaatttttcc tttaaaacca ctgatgatca
     2161 agggaaagac tacatcatca tgacgccgac catgatgtca gaagcctatc taggagagat
     2221 ttctcctgca caggttctag tagtagaacc aagaacgcgt agaatcgttg acggagtcgg
     2281 tcatttaact cgtgaaatca atatgcatga agcgatgtat gagactaatc cagctatcaa
     2341 agctgtccta catgctcatg cgcctaatgc aatgttctgg gcgactactg ggttgcctat
     2401 gcctaatttg acagaagcca cagctaaagt aaaagagatt cctgtcatgg actttgagcc
     2461 taattgctct gaagaacttg ctgaattgac aagcgactat gtaaaaaaac agcatctcga
     2521 attaccgcat atgttgctgc tcaactctca tggcgtattg atcaacacag gtggagaatc
     2581 aggaatcgaa gctatccata aagcactgca aatccttgat acagttgaat ggaatgcaga
     2641 aattgcctac aagcagacga tttttcaagc attagggatt actgacggtt tttattcaaa
     2701 aggtgagaaa atcgggggat tggaagagtt gaaacataaa acggctattt ataataaatc
     2761 tgttattgca gctggtggag actaacctat ctgacacaaa aaactacgac tttcacaaaa
     2821 tctgatccag atcgatatct gcttcaggtt gtattatgta agtcgtagtt cttttctttt
     2881 atttagttaa gaggtggtct gtccattccc tcgcttgctt gatttcgtcc atcgtcaact
     2941 gatgacctgt tacagttagc tgaatagtca catttgcttg gcgtgtttcg aattgatcgg
     3001 ccaattcttt aaaggaagca ggggaaacga ttggatcgtt atcaccataa gaaatccaga
     3061 cggttttatc ctctaatgtg gaacggcttt gatgaacacc tagagacatc ggatggaaaa
     3121 acaacccttt atttagctcg gtctcacgct ccaataataa atgagcagcg atgtttgcgc
     3181 catttgaata gccgacaagc agccagtcac ttaacggtat cagttctttt tcgctgattt
     3241 ctttgatttt ttcatggagt ttatctgttt ctttttccaa actgtccagg tcgaattgat
     3301 tcaatccatt acgcttgaaa aaacgattca tcccatcttc ttggaccgtg ccacgaaaag
     3361 aaagcaatgt actgtttggt gcaagaaagt gagcgatatc cagtaaagaa tgttcatcgc
     3421 cacctgtgcc atgaagtaag atcaaatgct tgccacttgt tcctttttca aaaatgtagg
     3481 tcattttgtt ccctcttttc tgtttctata gatgcaaatg aagtagcatg aagagattat
     3541 tcacgttttt tatttgcatc acttgtatcg aaaggacgga cataagcttc aatggcttca
     3601 cgacgttctt ctaagaatgg aggaagagat agtttttctc ctgctgattc tgctggttca
     3661 tcttcaagga atccaggacc atcagttgct aattcaaaca gtacgtgatg agcggctagg
     3721 aagtactcag attggaaata gaaacgatcg acgaaaccag aatttggcaa ttcaatctta
     3781 ttgattactt gaatccagta ttccaatgct tctttgtctg ctacacgcaa tgctaagtga
     3841 tggatatttc caaatccttc gattgctttt ggcaagtctg tccgatgttc aataatgatg
     3901 cttgcaccat ttcccccttc accgatttca aataagtaga aatctcctga atggtctgtt
     3961 tctttgaatc ctaagatttt ttcaagaatc aagcggatat gctctaattg actgacacgg
     4021 acaaagacag gtccaagtcc tgtgatcgca aactctgtcg gtacattgct gtttttccaa
     4081 ggaatgccag ctgctacacc ttgattgttt tcgtcagaga tcaattgata atactgatta
     4141 tcgaaatctt caaattcgat atattttttg ccgaaacgtt cattgattgc tgaatgagta
     4201 acgtcatatt tttcaaaacg ttccaaccaa aagtctaatg ctgcgtcact tggcacacgg
     4261 aaagaagcac gagagatcgt atctgttcct ttttttcctt ttggctgatt tggaaaatca
     4321 aagaaagtca tatctgttcc tggcgcacca gtatcgtctg taaagtaaag atggtaagtt
     4381 tcgatgtcat cttggttaac tgtttttttg attaagcgca ttcctaaaat atctgtaaaa
     4441 aagcggtaga tttttgtagc gctagatgtc attgctgtga cgtggtgaat tcctctgatt
     4501 tgtgtatcca tgataaattt cctcctgtta acttacaaaa agtaagtagt tgatttagac
     4561 atagtataga gggtgcatct ctattttgca attaatttgt cttctaaagt atcaggaagg
     4621 aatcaagaat ggaaaagagg agggacggat caataaaata aaaagactta cgactagctg
     4681 tatcgatttc aaaaaaacta gctttaaaaa gctaacttta gaatcattac gggtggtcat
     4741 aagtcttttt atgctgagtt attttttcat ttttgagcta atgcttagga tagaatagaa
     4801 tgttgatttc acgatctctt cttgttactt gaaactgctt accactgtca cgatctcttg
     4861 gtaaacggct ttttcacaac ctctttggtt aagttgaacg cacatccagc acagaatagt
     4921 tccgaacatc aaatcggttt cggctggaag aatattcaat cggctgtcca tctttgagat
     4981 agacgacttg ctgaacttcc agtacaggat cgttttctgc acagttcaaa tattgctgat
     5041 catattcatc acttttatct gcagaaatag cacgataagc ccctgcaaaa tggacattca
     5101 attcttgatg aagatgatca tagatagagg ctaacaaatg ttcttttttt aaacccggca
     5161 caagatggac tggcataaag gtatgctcta aaacatatgg ctcgccatct aagatcctta
     5221 accggtgaat attgtagaca ggttgctcgg catcgatcat caatcgttct tggatatatt
     5281 gattagggaa ttcaacatca aaatgaatga tttgactctt caaagaacga tgtctgcgtt
     5341 tcatttggag actcaaacct tcatactcgt tagcgggcga tgtgtctttt tgcataaatg
     5401 gatgattcaa gattttcgtt cctgatcctc gctgcgaata gacaagtcct tccattgcta
     5461 agatattgat ggcttttttg attgtcatcc ggctcgcacc aaattctttt accaagtcga
     5521 cttgattagg caaaaaacta tcgagaggat actcttttga acggatacgt tctcttaaaa
     5581 tatttgcgat ttcttcgtat tttggcacgt tattcacctc ttctagagta taacgaaaga
     5641 ttatgcttcc gacaactcga agtagcatct ggaacattgt ttattcgaaa aaagaggtca
     5701 tgacaagttt ttgtcacaac cccttaatga tctattaagc aatatctaaa tctggttgtt
     5761 tatctagtac tttcaggaat gggtaccaga taaaggcaac gatgacaaag tcgatgactt
     5821 gcagcatacc accaagccat gaatttgttg ctaagacacc gcttgcaatg attggcacag
     5881 tccatggaac agagacgcca gttggtgcag ggacaagtcc agcagccatt accaaataat
     5941 tgaacgtcgt tacgattaga ggtgccacaa cccaaggaat aaggattgtt gcattcaata
     6001 cgatcggtag accgaagatc gctggttcat ttacattgaa aataccaggt gctaaagcta
     6061 atcgtccaac atcacgatac tgtttctttt taagaacaaa ggccatcaat aaaacgacag
     6121 ctaaagtcat tccagagcca ccaataccaa cagtgaaggt ttccatgaat ggttttgtga
     6181 tgatgtgtgg taattctttt ccatgttgat aagcttctaa gttatcaagc atcagtgtat
     6241 tccaaatcgg gtccattaca gagttgacga tgatttgtcc gtgaagtccg aagaaccata
     6301 agaattggac aaagaacaaa gcaatcaatg ttgcaggtaa actacttccc aaaccagtta
     6361 atggtttttg gatcacttca taaacgacgt catgtaagtt agcattgaaa ataccagtca
     6421 tcacagcatt gattactaag aaaatagtta gtgtcactac agcagggatc aaagcggcaa
     6481 atgattttgt tactgcaggc ggaactccat caggcatttt gatctgccag ccgcgtttcg
     6541 tgattctgca gtagatttct ccagccaaga aagcagcgat cattccaatg aacattcctt
     6601 ttgcgccaag acgatcaagt gaaagcacac cagtcacatc ttcaccgcca gcggttttca
     6661 ttgcaaaagg cgtaagaatc aagaagctgg caaaagacac ggcaccaccg aaaatacctt
     6721 caacgtcata agattttgat aaataatagc cgataccaaa agtaacaaaa accgacatga
     6781 tgctcattgt tgcattttgc ccattaccga ataaattcga taaagttccc tttgttgcgt
     6841 cactgaaaaa aggcaagtta ttgataacga caacgatcga accgaacatc gttaaaggga
     6901 aggaaagcat gaaagcatcc cgcagcactg taaggtaacg attttgccct aataaattag
     6961 ctaagggcat gattttctca gaaagcttgt ccataaaacc gttcattttt attcacctca
     7021 aatttatttg caatcgatta catacaaagt ataacgccaa aaattttaaa tgtctagtca
     7081 ttttcataaa aagtattgat ttatttttta gggttcgtta tgattacggt atcaagaaaa
     7141 aagagaggaa gtaaaaaaat gaaaacctac acattcccag aaaatttttg gtggggttct
     7201 gctgctagcg gaccgcaaac agaaggaaga gtaagtgatg atggtaaagg agaaaatatt
     7261 tgggatcatt ggtatgaatt agagcctgaa aaattcttcg atcaagtagg acctgaaaaa
     7321 acttcgcaag tttacacaaa atacaaagaa gatgttcagt taatgaaaga aactggccat
     7381 aacacatttc gtacatctat ccaatggagt catttgattc cagaagggac aggtgaaatc
     7441 aatcaaaaag ctgtttcttt ttatcgagat tattttactg aattgctgga taatggtatt
     7501 gaaccttttg tgaaccttta ccatttcgat atgccactac cactgcaaga aaaaggcgga
     7561 tggttatcaa gagaaacggt cgacgcttat gtttcttatg cagcagcgtg ctttgaactg
     7621 tttggagatc tggtcaaaaa atggtttact cataatgaac caatcgtacc tgtagaagga
     7681 ggctacttgt atcaattcca ctatccaaat catatcaata tgaaagaagc ggtccaagta
     7741 ggctatcatg agaatctagc aagcgcaaag gtaatcaaag cctaccatga aggcggacat
     7801 gatggtgaga tcggaatcat cttgaatctg acaccaagct atccgagaga tgaaacgaac
     7861 ccagaagatg taaaagcggc gaagatcgct gacgcattct tcaaccgatc attccttgac
     7921 cctgcagtga aaggagagtt tccacaagag ttgatcgaaa ttgtcaaaga attggctata
     7981 atgccagaga tccatgaagg agacctagaa ctcatccgtg aaaacacgat tgacctgcta
     8041 ggaatcaatt actatcagcc tcgtcgaatc aaagcaaaag aaactccaat caatcacgaa
     8101 catagcccca tgccagatga ttattttgat aattacgaca tgcccaataa aaaaatgaat
     8161 ccatatcgtg gatgggaaat ttacgaaaaa ggaatctatg atattttaac gaatacaaga
     8221 gaaaactatg gtaatatcaa atgctatatt tctgaaaatg ggatgggggt cgaaggagaa
     8281 gaacgatttg ttaacagcga aggaattatt gaagatgatt accggatcga gtttgttacg
     8341 gatcatttga aatacgtcca tcaagcaatc caagaaggaa ccaattgtgt gggttaccac
     8401 atgtggacat gtatggacaa ctggtcttgg acaaatgctt ataagaatcg ctatggcttt
     8461 atcgctgttg atttggcaca agacggtaaa cggacgatca aaaaatcagg tcgctggttt
     8521 aaaacggtct ctgaaaaaaa cggatttgat gcctgatatt ttttgataaa acgttcacaa
     8581 aatgtttgtt ctaacactgt aatcgtgcta cactaacagt gaaggaggcg tttacaatgg
     8641 tagatactta tcgtaatatt ttagtagcaa ttgatggttc tgaaaaggca gaaaaagcat
     8701 ttcttgaggc aatcacgatt gccaaacgaa atcaggcaac gttgcacatc ctgtatgtca
     8761 atgaagtaac aggcaattac tttggtgatt ttgcatttgt tacaacaagc cttcaggaag
     8821 aattggatga ggttgcggaa aaccagatga aagaacatcg aaacttggca attgaaaaag
     8881 gcctgacaga tatcgagaca tatgttcttt acggttatcc aaagacactg attgctaatt
     8941 tccatgaatc gaaagaacca atcgatctga ttgtcatggg ggcgacaggt ctgaatgcgg
     9001 tagaacgtgc actagtaggt tccactactt cctatgtagt caaccacgca tcatgtaacg
     9061 tcatggttgt aaaataaata aattaaggga acagaaagca aaaaagcata acgcttcttt
     9121 tgctttctgt tccttttatt tttttgtaaa aaattacttt acctgatgaa gtaatcatgc
     9181 tattctaatt acaacgacag atggagtaat atggaggaaa gaaaatgatt tcaagtgatg
     9241 gaatacgcgg atacaatgat gcgattattt tatcgatcct taacaaaaac gattcttatg
     9301 gatatgaaat ttctaaaacg attcgtgaga ggacaaatga ttcctatgtg atcaaagaga
     9361 caacacttta ctccgttttt gcccgtttag aaaagaatca actgattgtt tcgtatcctg
     9421 gagaagtgac tcatggaaaa aagcggacat attataaaat cactgaaata gggcgtaatc
     9481 ttttagaaga aaaaaagcaa gagtggctga cgacgaaaga cgtagtcgat caatttttgg
     9541 agggaaatta aatggataca atccgtaatt atttagacag tctttttatc ggagtacctc
     9601 aaagtaccga gatagataag ctgaaaactg atttgttggc aaatatggaa gaccattatc
     9661 atgaattgat gggagaagga aaaaatgaac aggaggccat cggtacagta atttcgacat
     9721 ttggttcaat cgatgaatta ttagaagaac tagatgtcga aaaaaaacat caagctgacg
     9781 agacggaaac aaacacagct tctatttatt taagcgaagc agaaaactac tggaaagaat
     9841 acagagcggc aagtctccaa gtggcatcag gtgttctatt cattagctta tcttttgcaa
     9901 gttttctttt cttttgttca gcaggatatg tgttcatggg tatttcttgt ctgattttcg
     9961 gtatagcatt agctgtcggt ttttttattg cttctggtat gaaaatcaca cgtttaaatc
    10021 actttttgca ccatcggaaa atacctgaaa aagtattagc agaagcaaaa gaaaaagaag
    10081 aagagtatca gcgaagtttt ggcttctcac tgattgcagg aattggatta tgtatttttt
    10141 cacttttccc acttttggct tctttaatgt ggtatatgga tggtagtatc ggtgcctcta
    10201 tattttttgt tacggtagga acgggtgtct tcttgatcat ttatggcagt ctagtccgtc
    10261 attcttatcg tcaattcact caaagcgctt attactggtg aatgtattca gatttgtaaa
    10321 aatattctat ttatccggcg tagaaaaaaa gactgtgaca cgcttttgtc acagtctttt
    10381 ttatatataa taatttaaaa attcatcatc taaatccgta gaattttcta gattgcttac
    10441 ccatgtaaaa tcgttataga cagatttcag ttgacttctg cgccattgta cagggataaa
    10501 aagacgcatg ctatcttcat tgaatccgac taataatttc tttccgtcag taatgatggg
    10561 acgttgtaac aaagtaaaat gcgagacgat cagatcgaca agctctgagg tcgtacattg
    10621 gtcgatttgg tctttcaaat ttttataatc aatcgagcgt ttagctaata gatcgtcaaa
    10681 tccgttttct gttaatgaca ataaataaaa tatctctttc cttgttaaag gctgtttagg
    10741 tgtgaaaatt cgttctctgt aaggcatttg atggtctttc agccagtttg ccatcagatg
    10801 gtaactatgt gtgcgagaag aatagatcgt aagcataaaa aaactcctta aatagtccat
    10861 taatttgtta gatctgttga gataaataat aggtaaggag ctgtgcataa gcattgatca
    10921 aaacaaccca aacatcatta gaaataactt tctaaagtct taaacttgcg acgagaaaca
    10981 actagactaa tagaaaaaat gtcttagcca tttggcttcc tgtttgtttt tttgcttatg
    11041 ctgcagctcc tcgggaaagg gttctaacaa tttgaagaag gttgatgatt gatctagttt
    11101 aaattgtgtg aaatttgaaa tctggtgttt ttactagatg aatcacctaa caactatgat
    11161 tataacttat agaacttctg ttt
//
LOCUS       DACDKL010000083        10958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDKL010000083 DACDKL010000000
VERSION     DACDKL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.083"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..1179
                     /gene="lysA"
                     /locus_tag="IV329_001670"
     CDS             7..1179
                     /gene="lysA"
                     /locus_tag="IV329_001670"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8940846.1"
                     /translation="MRQTMLSFKRGFVSKRFDTKVLYASKAFQTIEMLHLVREYGFGL
                     DVVSGGEIYTALQADFPRDTIYFHGNNKTPEELIYALENDLLHIVADNLMEVELLAEL
                     SQRYLKKICIMLRLNVGVEAHTHEYIVTAHIDSKFGMSYESKECQQALQLIKESSYLE
                     LEGFHAHIGSQIFDVTAWLAEIDKLTNYLTDFDQPLSLNLGGGFGIRYTQNDQPIPVE
                     EALKKLIEYTEEALEKRKLTIRQLLIEPGRSIVGEAGTTLYTIGFIKKTPHKKYYFVD
                     GGMTDNIRPALYQAEYDCDLAAKLSEEKTEKVTIAGKMCESGDVVIKEAYLPKAESGD
                     LLAVYSTGAYGYSMSSNYNRATRPAVVFVNNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1166..1966
                     /gene="dapF"
                     /locus_tag="IV329_001671"
     CDS             1166..1966
                     /gene="dapF"
                     /locus_tag="IV329_001671"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8940847.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            1969..2850
                     /locus_tag="IV329_001672"
     CDS             1969..2850
                     /locus_tag="IV329_001672"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8940848.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2847..3563
                     /locus_tag="IV329_001673"
     CDS             2847..3563
                     /locus_tag="IV329_001673"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8940849.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3569..4264
                     /gene="dapD"
                     /locus_tag="IV329_001674"
     CDS             3569..4264
                     /gene="dapD"
                     /locus_tag="IV329_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8940850.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4280..5281
                     /locus_tag="IV329_001675"
     CDS             4280..5281
                     /locus_tag="IV329_001675"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8940851.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5278..6639
                     /locus_tag="IV329_001676"
     CDS             5278..6639
                     /locus_tag="IV329_001676"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8940852.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6599..7750
                     /locus_tag="IV329_001677"
     CDS             6599..7750
                     /locus_tag="IV329_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8940853.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7725..8894
                     /locus_tag="IV329_001678"
     CDS             7725..8894
                     /locus_tag="IV329_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8940854.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9069..10448
                     /locus_tag="IV329_001679"
     CDS             9069..10448
                     /locus_tag="IV329_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8940855.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10729..10958
                     /locus_tag="IV329_001680"
                     /pseudo
     CDS             10729..10958
                     /locus_tag="IV329_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3625 a         1926 c         2465 g         2942 t
ORIGIN      
        1 gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat
       61 acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt
      121 gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct
      181 gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc
      241 tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta
      301 ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc
      361 ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc
      421 atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat
      481 ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg
      541 ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg
      601 aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa
      661 gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc
      721 attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta
      781 tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg
      841 atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca
      901 aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc
      961 gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt
     1021 tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca
     1081 gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct
     1141 gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga
     1201 ttttatctta ttagattacg cagaagacac agattatagt caattagccg cttctttatg
     1261 cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat
     1321 gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt
     1381 tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt
     1441 agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat
     1501 gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa
     1561 ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat
     1621 tcatacagtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact
     1681 gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa
     1741 tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg
     1801 tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca
     1861 ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat
     1921 ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt
     1981 tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg
     2041 gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca
     2101 acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca actgacagta
     2161 aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa
     2221 acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc
     2281 ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa
     2341 gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca
     2401 atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca
     2461 ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac
     2521 agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt
     2581 gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga
     2641 agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc
     2701 agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga
     2761 cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt
     2821 ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg
     2881 gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg
     2941 cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt
     3001 ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag
     3061 tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac
     3121 atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa
     3181 tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata
     3241 gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag
     3301 tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg
     3361 gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta
     3421 ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg
     3481 cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt
     3541 tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca
     3601 aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt
     3661 taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac
     3721 gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc
     3781 tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc
     3841 aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc
     3901 gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt
     3961 aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg
     4021 tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc
     4081 gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc
     4141 agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt
     4201 aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt
     4261 atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt
     4321 agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa
     4381 attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac
     4441 tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg
     4501 tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga
     4561 taattcctca gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct
     4621 ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt
     4681 acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta
     4741 tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga
     4801 cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca
     4861 gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat
     4921 tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg
     4981 acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga
     5041 gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc
     5101 gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac
     5161 ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc
     5221 tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg
     5281 agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc
     5341 aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct
     5401 ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct
     5461 taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt
     5521 caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag
     5581 aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg
     5641 actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt
     5701 ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca
     5761 tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa
     5821 gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg
     5881 caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga
     5941 atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct
     6001 tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc
     6061 cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac
     6121 gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc
     6181 actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt
     6241 tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg
     6301 atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa
     6361 acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg
     6421 ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta
     6481 gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta
     6541 ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat
     6601 gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa
     6661 gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg
     6721 cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac
     6781 agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga
     6841 aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga
     6901 ctttataact aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc
     6961 agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga
     7021 agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc
     7081 tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc
     7141 tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt
     7201 gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt
     7261 tggccaaatc caaggtggaa cgatccgcaa tgttttagct ggagaagtta ttgtatccgg
     7321 aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt
     7381 agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc
     7441 tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt
     7501 agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt
     7561 atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga
     7621 tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct
     7681 tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca
     7741 atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc
     7801 aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc
     7861 ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg
     7921 gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg
     7981 gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg
     8041 cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt
     8101 attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt
     8161 ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat
     8221 cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg
     8281 caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg
     8341 atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc
     8401 cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag
     8461 tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc
     8521 tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg
     8581 tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc
     8641 aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca
     8701 aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg
     8761 caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga
     8821 tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt
     8881 taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt
     8941 ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9001 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9061 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9121 cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9181 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9241 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9301 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9361 catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9421 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9481 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9541 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9601 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9661 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9721 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
     9781 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
     9841 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
     9901 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
     9961 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10021 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10081 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10141 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10201 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10261 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10321 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10381 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10441 tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta
    10501 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg
    10561 cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt
    10621 accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt
    10681 taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga
    10741 ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag
    10801 cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc
    10861 aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg
    10921 cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DACDKL010000084        10690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDKL010000084 DACDKL010000000
VERSION     DACDKL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.084"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV329_001681"
     CDS             complement(25..144)
                     /locus_tag="IV329_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8940856.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV329_001682"
     CDS             complement(159..1343)
                     /locus_tag="IV329_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8940857.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IV329_001683"
     CDS             complement(1599..1853)
                     /locus_tag="IV329_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940858.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IV329_001684"
     CDS             complement(1878..3020)
                     /locus_tag="IV329_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8940859.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IV329_001685"
     CDS             complement(3080..4432)
                     /locus_tag="IV329_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8940860.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IV329_001686"
     CDS             complement(4503..4847)
                     /locus_tag="IV329_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8940861.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IV329_001687"
     CDS             complement(4857..5231)
                     /locus_tag="IV329_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8940862.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IV329_001688"
     CDS             complement(5244..5558)
                     /locus_tag="IV329_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8940863.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV329_001689"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IV329_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IV329_001690"
     CDS             complement(8976..9638)
                     /locus_tag="IV329_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940864.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IV329_001691"
     CDS             10255..10485
                     /locus_tag="IV329_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940865.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2953 a         2364 c         1809 g         3564 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt
//
LOCUS       DACDKL010000085        10576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDKL010000085 DACDKL010000000
VERSION     DACDKL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.085"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            81..2141
                     /locus_tag="IV329_001692"
     CDS             81..2141
                     /locus_tag="IV329_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940866.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2224..2793
                     /locus_tag="IV329_001693"
     CDS             2224..2793
                     /locus_tag="IV329_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8940867.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2968..3774)
                     /locus_tag="IV329_001694"
     CDS             complement(2968..3774)
                     /locus_tag="IV329_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940868.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3912..5426
                     /locus_tag="IV329_001695"
     CDS             3912..5426
                     /locus_tag="IV329_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8940869.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5829..6812)
                     /locus_tag="IV329_001696"
     CDS             complement(5829..6812)
                     /locus_tag="IV329_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8940870.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6817..7464)
                     /locus_tag="IV329_001697"
     CDS             complement(6817..7464)
                     /locus_tag="IV329_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8940871.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7549..8181)
                     /locus_tag="IV329_001698"
     CDS             complement(7549..8181)
                     /locus_tag="IV329_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8940872.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8197..8748)
                     /gene="coaC"
                     /locus_tag="IV329_001699"
     CDS             complement(8197..8748)
                     /gene="coaC"
                     /locus_tag="IV329_001699"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8940873.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8745..9482)
                     /locus_tag="IV329_001700"
     CDS             complement(8745..9482)
                     /locus_tag="IV329_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8940874.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9479..10468)
                     /locus_tag="IV329_001701"
     CDS             complement(9479..10468)
                     /locus_tag="IV329_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8940875.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         3410 a         1995 c         2112 g         3059 t
ORIGIN      
        1 tgattttatt tatataatga aatcatctta tgaaaattac tacttcatgt ttagacaaga
       61 gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta aataaacacg
      121 cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc
      181 gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt caggcaacga
      241 cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc cctatcacta
      301 ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg acttcctata
      361 ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac aaaacattat
      421 ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg actgatccta
      481 caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg acagatataa
      541 aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca cagtttgaaa
      601 gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg gtagaaaggc
      661 tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac aatgggaata
      721 tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt gatccaattg
      781 ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa acagctatca
      841 aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat cttgttgaat
      901 atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg aacgggacag
      961 tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac tcgaaaacat
     1021 caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct tcatcgactg
     1081 tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa acacatatga
     1141 gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat cgagtgccaa
     1201 atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct gattggatgc
     1261 aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta cttcactcag
     1321 aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat gtttatatac
     1381 cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa aaaataaaca
     1441 aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg
     1501 tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa caaacaggtg
     1561 attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc catgctcagt
     1621 cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa aaaagacgag
     1681 aagaaagctt gtcagaaaaa aaacaacaaa tggctcatgc agtttctcca acagggaaag
     1741 taaaacaaac aagaagccta gagttagcaa gaatatctag aaaaatctca gaatctaata
     1801 aaacgataag gcaagaagct catttggccc cacaagtttc tggattggaa aatgaaattt
     1861 tagagcctaa aataaaacaa caatcagaaa aagaatttgt tgctaaacag tcggaagatc
     1921 agaaggaatc agatgaccag aaggaaacga agaaaaaacg cttgtcaact attgattcca
     1981 ccgatgagca tctgcagccg caagtaccac ttcctagtcc gggaacaccc gcacctggtg
     2041 gaggtattcc ttcttctgct ttaggagatt tcatgcgagg tatggcagga acatgggtat
     2101 acgctaatcg aaatgaattt gacgcagatg actggttctg atttatatct ctatctgtta
     2161 tagtcaaata agtagctgag tcatatgtta tttggcataa tggagacgag gagggataga
     2221 aaaatgttag ataaaacgtt accttacgca gaaatctgga tgacaagacc acttcaacaa
     2281 aatgttccaa tcacgccttt agcggaaggt tttgaatttc gattttatca aaaagaagat
     2341 gaatctgctt gggcggagat tgagacttcc gttggggaat ttcaaacgac agatgaagca
     2401 cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag aacttgaaag aaggatgttt
     2461 tttgtagaga caaaaaaagg agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca
     2521 agagagcaag tgtatccggt ttttcattgg ttagcagtga aaccagatta ccaaggaaaa
     2581 gggctcgcaa aagcattgac tgccagagtc ttgcaagaat ttcctaccct ccatacggac
     2641 ggaccaatct atcttcacac acagacatgg agccatcagg cgattgctct ttataaaaaa
     2701 atgggtttta cttttattgc tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa
     2761 gttatgcagg ttttaagtaa attagataaa taacgaaatg gtgaaataag tcctcagtat
     2821 tgggataaaa aacgaaatag tcattcctca tatcttactt ttttgagtta atactatcgg
     2881 ataactaatg gcatgccata cattcaaaaa agaaggggca gtgacaaaag tattgtcact
     2941 gccccttctc ttgtttttta gcatgtttta tcgtttgatt tttttaggaa taaaattctt
     3001 gatgaacgta aatttttggt aatcgtttcg actatagagg taaccaaata cactgaaaac
     3061 aactgctcca ataaaattaa caaataaatc cttcatagta tccatgattc ccacgtctaa
     3121 gtaaccattt tggatgattg ttttatctgt tttaccatct gcattttgac tttcaatgat
     3181 tgtccgagtg atatctggaa catgatagac agtgtttccg tctttactta actccacact
     3241 attgatttgc tggatgatcg tatctttttg catatcgaat tgcagtaatt tatctgctga
     3301 aaattccaca aactcccaca taacaccaac tgtcatcgaa aaacagaagg aaacaactgc
     3361 tagtaataaa ggagtcaagt tcatggtttt tgtattttta ttgagcaaat aaacaaggga
     3421 aaagccgaca cccccagcta aaaatccgct gaatgcatgt agaaaagtat cccacattga
     3481 aaaatagcca taaaaatcac ttaactctcc taaaactgtt gaactaaaaa taaaagataa
     3541 caaaatgatt tctagcatat ttggaatctg tatctttaat atcttttcca gaaaataagg
     3601 gatttcaaat aaaattaatg tcagtaataa aagcattgta tttccccagt gttggttcca
     3661 gacctgatcc actagcataa taacgacgat gatacgtaat aaaatataga ttccgtttgt
     3721 gattctacct gcagtttttg tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata
     3781 actccttcat gtatatcctt aattatacta agaagcgtgt gtttaccaaa gctatatgac
     3841 ttaaagaaaa acagagttga tattgtgaaa aaaatcactt aatatagtat aaggagaaaa
     3901 gggggataca aatgaaaaaa ttagttgctg gtttattaac agccgttgca cttcttgcta
     3961 tgagcgcatg cggcgcagat cagaaaaata cagcgcagac aagttctagt acgaaggaat
     4021 ctgccgaagt atcatcaggc gcttctgaac aagtatatac agatccaaaa gaaatgaaag
     4081 aatcttatga tgtcgtgatt attggagctg gtggtgcagg gatggcagca gctatcgaag
     4141 caaaagatgc tgggttgaat ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca
     4201 catctaaatc atcaagcggt atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa
     4261 tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg
     4321 ataaagaaat gcttcgatac tttgtcgatc actctgctga tgcggtcgat tggctagatc
     4381 aaaacggtat caaactggac aacttaacga tcactggagg gatgagtgaa aagaggacac
     4441 atcgtccgtc tgatggctcg gctattggtg gatacttagt ggaggggtta ttgaataacg
     4501 tccatgagcg ggaaatccct atttttgtca acgcagatgt gacagacatt aagaaaaatg
     4561 gggaaacggt caatgaagtg actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg
     4621 gaaaagcaat cgtagttacc acaggcggtt ttggtgccag caaagaattt attgaaaaat
     4681 atcgtcccga tttagctgat tatattacga caaatcaaga aggatcaact ggagatggta
     4741 tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat ggataaaata caaattcatc
     4801 caactgtgca gcaggatgag ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa
     4861 tcttggtcga tcaagagggg aaaagatttg tcaatgaaat ggatacaagg gataaagtat
     4921 ctgcagcaat tacagcattg cctgaaaaat cagcttattt gatttttgac caaggcgtgc
     4981 gtgatcgtgc cacagctatt gaattttatg accaaaaagg atatgtcaca actggaaaga
     5041 caatggaaga attagcagaa aaaatcgatt taccaaaaga aacactagca aaaacagttg
     5101 atggatggaa tacggatgtt gcggagaaaa aagatgctca attcaaccgt gccacagcca
     5161 tggaacatga cttatcgtct ccgaattatt atgcgatcaa aatcgcacca ggaatccatt
     5221 acacgatggg cggtgtgaaa atcaatacga acactgaagt attagataaa gaaaaccagc
     5281 caattagggg gttatatgct gctggtgaag tcacaggtgg cttacatgga aacaaccgga
     5341 tcggtggaaa ttcagttgcg gagatcatta tttttgggcg acaagcgggc atacaagcag
     5401 ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca
     5461 gcgttttgag ctaataccga gggtcagctg attgaacact gtttagtgaa gaggttgtga
     5521 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     5581 aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tcggaaaaag
     5641 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     5701 aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     5761 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttgatca
     5821 gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt tgcttccata
     5881 tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc
     5941 ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag ctctgcttta
     6001 tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt cccaatcaat
     6061 acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa gacccaacgt
     6121 ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat gaaatccaaa
     6181 ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt
     6241 ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt ttcttctgat
     6301 cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat cgctgctgtt
     6361 cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa ttttccaagc
     6421 cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc agaaacatag
     6481 tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata ttcggcataa
     6541 ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga gatatcactt
     6601 ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg aggtttcaaa
     6661 gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg gactttgatc
     6721 agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa tacatctaca
     6781 gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg cccattcttg
     6841 gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac gagaaatatc
     6901 cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga tcgtttttga
     6961 tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt
     7021 gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat ctgcataatg
     7081 tttagtaata taataatctg tcatggactc aggccatttt gctggttcat ctgcataaac
     7141 agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca ctgttttgat
     7201 tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag caaaaatcaa
     7261 tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta aatcaaaaga
     7321 gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc tcacccctgc
     7381 aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt
     7441 tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc tttccattca
     7501 atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc agttagtaag
     7561 agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg caagacctgc
     7621 gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg aataaacagc
     7681 atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt agatcatatt
     7741 catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata aagaaacgga
     7801 atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag gaaccacccc
     7861 aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat gttctgttcc
     7921 gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa ctgtgctacg
     7981 gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac
     8041 gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac cgatagggat
     8101 aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg cagttaatgt
     8161 aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt cattaagagt
     8221 cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc caaaatctcc
     8281 acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc tcaatgtttc
     8341 cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag gggcaatcaa
     8401 ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc
     8461 gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg
     8521 ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga cgtctgtatg
     8581 gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg tgctgctttt
     8641 agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa gatcagcgct
     8701 tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt tgattcctcc
     8761 agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc ttcttccact
     8821 gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc cgtcaaatca
     8881 tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac tgccagaaga
     8941 gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt tggctgccat
     9001 tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg
     9061 ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa ccaattgtcc
     9121 agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc tgaaaaacta
     9181 aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta gtgttctttt
     9241 ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt ataaatcgtt
     9301 atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc gattgtggta
     9361 tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt cgaatgattt
     9421 gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat tagaatcttc
     9481 atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca agtagtttag
     9541 atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca tctgcaatct
     9601 gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca ttgacagaat
     9661 caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga
     9721 ttcccccagc attgccacat aaagctactg caccgttata agagatcaat ggaagaagac
     9781 tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt tttcctaaag
     9841 cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca cctagacttt
     9901 ttagccatgt gacagactct tcttttctgg aaaaggcaat gattgactgg aatcctagtt
     9961 tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta acgacaactt
    10021 ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata gcagtaaacc
    10081 ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga ggaactaacc
    10141 agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg ccaagcccaa
    10201 aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt tggacgacag
    10261 ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga ccagactcgc
    10321 tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc aagacttcat
    10381 tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca ggttccttta
    10441 ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa ttttctttta
    10501 tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac aaaaattagg
    10561 tgaaaaataa agaatc
//
LOCUS       DACDKL010000086        10573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDKL010000086 DACDKL010000000
VERSION     DACDKL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.086"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..293
                     /locus_tag="IV329_001702"
     CDS             126..293
                     /locus_tag="IV329_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8940876.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(355..2166)
                     /locus_tag="IV329_001703"
     CDS             complement(355..2166)
                     /locus_tag="IV329_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8940877.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2163..2528)
                     /locus_tag="IV329_001704"
     CDS             complement(2163..2528)
                     /locus_tag="IV329_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8940878.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2691..3086)
                     /locus_tag="IV329_001705"
     CDS             complement(2691..3086)
                     /locus_tag="IV329_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8940879.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3104..4066)
                     /locus_tag="IV329_001706"
     CDS             complement(3104..4066)
                     /locus_tag="IV329_001706"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8940880.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4066..4278)
                     /locus_tag="IV329_001707"
     CDS             complement(4066..4278)
                     /locus_tag="IV329_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8940881.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4299..4997)
                     /locus_tag="IV329_001708"
     CDS             complement(4299..4997)
                     /locus_tag="IV329_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8940882.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5017..5559)
                     /locus_tag="IV329_001709"
     CDS             complement(5017..5559)
                     /locus_tag="IV329_001709"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8940883.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5691..6695)
                     /locus_tag="IV329_001710"
     CDS             complement(5691..6695)
                     /locus_tag="IV329_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8940884.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6692..7681)
                     /locus_tag="IV329_001711"
     CDS             complement(6692..7681)
                     /locus_tag="IV329_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8940885.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7678..8484)
                     /locus_tag="IV329_001712"
     CDS             complement(7678..8484)
                     /locus_tag="IV329_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940886.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8650..9606
                     /locus_tag="IV329_001713"
     CDS             8650..9606
                     /locus_tag="IV329_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8940887.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9683..10201)
                     /locus_tag="IV329_001714"
     CDS             complement(9683..10201)
                     /locus_tag="IV329_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8940888.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10289..10438)
                     /gene="rpmG"
                     /locus_tag="IV329_001715"
     CDS             complement(10289..10438)
                     /gene="rpmG"
                     /locus_tag="IV329_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8940889.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3294 a         2302 c         1804 g         3173 t
ORIGIN      
        1 taataaaaca tttagtaaac attttcaagg gttttttgca aaggtttaca aacaaacgta
       61 aaactagcct ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg
      121 attcaatgaa aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg
      181 ttgctggttt ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag
      241 caatgatcga agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat
      301 ttaaaaatac gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt
      361 gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata
      421 aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata
      481 aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc
      541 tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt
      601 gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa
      661 tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg
      721 gttgtgcttt tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat
      781 cagtgctgct aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag
      841 atgagtaaac catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc
      901 aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc
      961 tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc
     1021 ttctgaaaag ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct
     1081 ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc
     1141 atgctcaaaa tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata
     1201 aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga
     1261 ctgatgccat tgctccaggt tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc
     1321 ttgcttttga tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac
     1381 aatcggttcc ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc
     1441 tcctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa
     1501 aaagacattt tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag
     1561 ctgatacagt aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc
     1621 atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc
     1681 tgtctgtaaa agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa
     1741 ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc
     1801 gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag
     1861 ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg
     1921 aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag
     1981 cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac
     2041 tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt
     2101 attatggaga atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat
     2161 tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt
     2221 tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt
     2281 ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca
     2341 aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca
     2401 atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat
     2461 ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat
     2521 ttgatcatag ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag
     2581 cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc
     2641 aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt
     2701 ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga
     2761 taacctgctt tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa
     2821 atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg
     2881 atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt
     2941 acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt
     3001 ttggccatta ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa
     3061 atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg
     3121 ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg
     3181 ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta
     3241 aaaattctcc tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat
     3301 tccccaagtt tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg
     3361 caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt
     3421 tgtctagcat tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta
     3481 ggcgtaccac acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac
     3541 attcgaaccc ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc
     3601 cgtgaatcaa atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga
     3661 atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat
     3721 tcgcgtcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa
     3781 attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca
     3841 cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat
     3901 tcaaacgatg attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa
     3961 tattggtatc gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt
     4021 ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt
     4081 ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt
     4141 atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat
     4201 gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac
     4261 atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt
     4321 tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc
     4381 ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact
     4441 aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca
     4501 aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg
     4561 ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta
     4621 cttgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt
     4681 atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc
     4741 tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg
     4801 aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt
     4861 ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc
     4921 tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatgcacg caattttatt
     4981 tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat
     5041 cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct
     5101 acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat
     5161 tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg
     5221 caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga
     5281 ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat
     5341 tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt
     5401 cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt
     5461 atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc
     5521 gattcctttc cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc
     5581 ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat
     5641 agaataaaaa gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc
     5701 agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca
     5761 atcaaaattc ctgtaggtac ttccccactt ggtagaatca ctctagcaac catatctgca
     5821 ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta
     5881 cgtacgacta gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc
     5941 accgctacac taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta
     6001 gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc
     6061 tgtgcataaa gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca
     6121 taaggccagc tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt
     6181 tccttagata attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca
     6241 ataccggaca ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga
     6301 tagacacagc aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc
     6361 agcgaatagt gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta
     6421 atgcccaata tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca
     6481 gaaacgccaa gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct
     6541 ctaacaatca gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc
     6601 aggtaaataa aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa
     6661 agcaagaaat agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca
     6721 gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat
     6781 tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga
     6841 caggtagaac ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca
     6901 gcccaataaa tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta
     6961 gacagaaaac aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt
     7021 cactcaaatg aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta
     7081 tatataaagg aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa
     7141 accaaaaagc aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag
     7201 taaaaaaaga actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt
     7261 tcgaaaactg caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca
     7321 ttagaaacac agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca
     7381 acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcaccc
     7441 cttgcatcaa tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac
     7501 gaggcaaacg aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca
     7561 acgcctgcca aaaatctgct ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa
     7621 aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca
     7681 taattgatcc tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt
     7741 tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt
     7801 gatcacttct tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata
     7861 atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt
     7921 catttgattg atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa
     7981 tgttgtcggt tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca
     8041 gacacgctgg gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag
     8101 acctgtagca tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa
     8161 cagtttttga taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg
     8221 gtgttcacta gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt
     8281 agagatatca gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa
     8341 acttttcaat aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc
     8401 ttcaggaatc gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat
     8461 gtcagctgct cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta
     8521 tgacgctgtt tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt
     8581 tgacagcgtt cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg
     8641 aggaaagaaa tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct
     8701 ggagtactga gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg
     8761 acgacttcat ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt
     8821 ccaaacgcgc ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg
     8881 gaaaaaccta tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat
     8941 gatttaaagg atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt
     9001 gagcctgatt tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa
     9061 tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa
     9121 caactaaaag atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc
     9181 gattatcaaa aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag
     9241 acagctgcag tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca
     9301 agtggaagaa tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc
     9361 tccaaaaaag ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat
     9421 gctgactaca tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc
     9481 tggaagaatc taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt
     9541 tggctatata acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta
     9601 aattgatttt aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat
     9661 taacaaacta gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt
     9721 cccatttttg aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct
     9781 ctataagggc gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc
     9841 tatcgatcaa tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac
     9901 taccacccga aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga
     9961 tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga
    10021 tgatatggcg ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca
    10081 ctaaaattcg atccattgta tgttcgcgaa agaaacgcat atcatttggc aatcgccatg
    10141 gtaacaaacc atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca
    10201 tattatccgc tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac
    10261 cagcaaggaa ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc
    10321 gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc
    10381 ttgttagata gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt
    10441 ttttccctcc catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc
    10501 atgatttttt ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga
    10561 taaatacaac ggt
//
LOCUS       DACDKL010000087        10231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDKL010000087 DACDKL010000000
VERSION     DACDKL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.087"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(255..2375)
                     /locus_tag="IV329_001716"
     CDS             complement(255..2375)
                     /locus_tag="IV329_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8940890.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2605..3783
                     /locus_tag="IV329_001717"
     CDS             2605..3783
                     /locus_tag="IV329_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8940891.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3799..4557
                     /gene="aroD"
                     /locus_tag="IV329_001718"
     CDS             3799..4557
                     /gene="aroD"
                     /locus_tag="IV329_001718"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8940892.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4580..5065
                     /gene="ybaK"
                     /locus_tag="IV329_001719"
     CDS             4580..5065
                     /gene="ybaK"
                     /locus_tag="IV329_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8940893.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5136..6389)
                     /locus_tag="IV329_001720"
     CDS             complement(5136..6389)
                     /locus_tag="IV329_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8940894.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6507..7856
                     /gene="gdhA"
                     /locus_tag="IV329_001721"
     CDS             6507..7856
                     /gene="gdhA"
                     /locus_tag="IV329_001721"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8940895.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7968..9314
                     /locus_tag="IV329_001722"
     CDS             7968..9314
                     /locus_tag="IV329_001722"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8940896.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9622..10008)
                     /locus_tag="IV329_001723"
     CDS             complement(9622..10008)
                     /locus_tag="IV329_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8940897.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10057..>10231)
                     /locus_tag="IV329_001724"
     CDS             complement(10057..>10231)
                     /locus_tag="IV329_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8940898.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3280 a         1851 c         2081 g         3019 t
ORIGIN      
        1 aatcgagcag tagcatagcg gattattttc atacttttca cctctataaa aagtatagaa
       61 aatgaatatg aataaagtat gaaattcctt tagtgaaatc acgaattaaa accagaaaat
      121 attttgtaaa aagcaagagg ttgtgaagaa gctgccctat tctattaccg tagatgcaag
      181 cttttgaaca ctgtttattc gaaaagagga gcagcgacaa taattttgtc gctgctcctt
      241 ttaaatttat tttgtcattc ggaagaactt gtggtcgttc cgtgaagttc ttggtacgtt
      301 tgcgttttat aaagatcaac agttgattgt ccgttgtgtt cactaaagac actagttgac
      361 ttgtcaccaa gagatttttc agcttctttt aatttctcta agccattctt gtagtcataa
      421 tcactggtat caattgggtc taatccacta ttcgtataga accgaagaag atcaccatta
      481 ttgatttggt cagaaacagc taactgttta tcaacttttt ctttcagtga atcaatttca
      541 ttctgtactt cctcagtagg ttcagtaata gcttcaccag tttcgttact atagatcgta
      601 ccaccgtagt aagtatattt tggtgtaaca aaatcaccat cacggaaaga aacaagctgt
      661 tcatgatctt tcgagaatag atcttgtcct aactgaatat aattttttgt atctacacct
      721 agtaagtgaa gtaatgtagg caaggcatca acttgtccgc cgtaagtgtg attgattccg
      781 ccctgtgttt gtccagggat atggatcata taagggacac gctgcatttg tgcattgtcg
      841 tagtttgtcc aagtagaact tgtttttcca actaattcag ctagattttt atttcttgaa
      901 ttcgagacac cgtaatggtc accgtaaagg acgatgactg aattatcata caagccgctt
      961 tctttcaaat aattgaagaa ttcttgtacg gcggtatcta aataattggc tgtcgcaaag
     1021 taaccgttga tcgtttcatc atttgtattg gctaacggga atcctgcttc gtcattggtg
     1081 aattttgaat aaggataatg gttagataca gcaatgaatt ttgaataaaa tggttgttgc
     1141 agacgttcca agtattgtac ggattgttgg aaaaatggtt tatcatgcaa cccgtattgg
     1201 aacgaattgt catcattaac atcataataa gaagaatcaa agaaataatc atatcctaaa
     1261 ttcttgtatg tctcgtttcg attccagaat gtaccagcgt tcccatggaa taccgcacta
     1321 gtgtaacctt gtgtttgact taaaatatca ggtgcagctt ggaaagtatt ttttccgcct
     1381 aattgtgtga acaaggagcc ttgattcaag ccaaacaatg aattttccat cagcgtttcc
     1441 gcatcgcttg tttttcctgc ttttacttga tggaaaaagt tgtcaaaact gaacgtgctg
     1501 ttgctgtggt aaagactatt gatgaaaggt gtaacttcat gctggagacc attctcatct
     1561 gtcaatttat aatcgatcag gaattgctgg aagctttcta aatggatata aatcacattt
     1621 tttcctttag cgagcccgaa caaatcgtca tttggtgctg cataatgttc ttttacataa
     1681 ttttctactt ctttcatatc attgggactt gcttctgccc gcacttggtt tgtctggtaa
     1741 gtttgaacac cgtcataaac catgaaagca ttcaagccaa gatatttgac caaataatca
     1801 cgagagaatg ttcgagtcag caattctgga cgatcagctt ccgccataaa aagatttcct
     1861 gagaaaatca taactgctaa tgtagagatc gcaaaagcca ttcttgctcg gatcgggcga
     1921 gggtccaact tgattttttt tgcaatcagc agaacagtca agataataaa atcgatccaa
     1981 tagatcacat catatgggcg aaacagtcga atggcacttt ctcccagacc actcgctact
     2041 ttcccagcac ctaaaatcgt gttgatcgta atgaagtccg tgaattctcg ataataagtg
     2101 acattcgaaa aaacaagcaa tgtcaaaaga aaataaatga ccatcatcgt gatataagca
     2161 actctagggc gtctgatata aagagcaatt ccaagtaata acaatgtagt tgcaataggg
     2221 ttgataaaca agataaagta ttgaatgcca ctttcgattc cgagatggaa atcaactaaa
     2281 taggcaaaaa tcgttttcaa ccaaagtaaa acgactagta aagaaaaaaa tccgagcctc
     2341 ttattcaaaa aggcaggtgt ttttatacat ttcaagataa tcttcctttc tgcacttctt
     2401 aatcacagaa gtgtcatgaa accatgttac acagctcact tattctcatt tattttacta
     2461 gtatgacaat atagtgtcaa ttgaaaccag aagtttatgc tgtttcatta aggttttctt
     2521 taagcttcat gaaaaaatga gcagtaagac aagtcctatg ttttttgtta tacttgggaa
     2581 gagaaagcta aagcaggtga aggaatgaaa gtaaaagtaa aaaaaagagc aagcaatcga
     2641 ttgaaaaaag gatatccact gatccaaaaa gaagaccttg ctcatcccca aaaagaaacc
     2701 acgcaatggg tggaattagt tgattcgcaa aaccaattta tcgcaaaagg atatctagga
     2761 aagcagaata agggaatcgg ttgggtattg agccagcaga acatcccatt tgatcaatct
     2821 ttttttgaag aattttttgt gaagtcaaaa gaaaaaagaa ctttcttttt ttcagatgat
     2881 tctactaccg cttttcgtat attcaatgga gaaggagacg gaatcggcgg tctgattgtt
     2941 gattattatg caggttatgc tgttttttct tggtataacg aaacgttata tgcacatcgt
     3001 gaattattga ttcaagcttt tcgtgaaatc tacccggaaa tcgtaggagg ctatgagaaa
     3061 attcgatttg aatcagtaaa tctgcctgag agccagttca tttacggaaa agaggcaccg
     3121 gaacccttga tcgtaaaaga aaacggagta aaatttgccg tttatttaaa tgaaggatta
     3181 atgactggga tctttttaga ccaaaaagaa gtacggggat cattagcaga aggtttcgct
     3241 tctgggaaaa cagttttgaa catgtttagt tataccggcg ccttttctgt tgctgccgca
     3301 atgggagggg caatcgaaac aacaagtgtc gatctagcaa aacgcagttt aaaaaaaaca
     3361 caggaacagt ttgaagtgaa tggtttggat gtcgatcagc aaaaaatcat tgttatggac
     3421 gtattcgagt atttcaaata cgccaaaaga aaacaatttt tatatgacat gattgtattg
     3481 gatccaccaa gttttgccag aaataagaaa aaagtctttc gcgtagcaaa agattatggg
     3541 gaactggtca aggatagtct ggatatttta gcagaagacg ggatacttat tgcgtctaca
     3601 aatgctgcga atgtctcgat agaacaattc caaacaatga ttgaagagga attccatgcc
     3661 aaaaatgttt cttttgaaca acttgcttta tatcgtctgc caaaagattt ccagacgacg
     3721 gatacctttt tagaaggcaa ttatctgaaa gtgtttgttt atcaaattaa aaataaccat
     3781 tagaaggagc gatgcagaat ggtttgggta aagggattga atataggaag cggtagacca
     3841 aagatttgtg cgcctcttat cggggccgtc cgagaggaag tattacgtga agcagttctt
     3901 gcacgtgaag caggggccga tttagtcgaa tggcgagtcg atcattatcg tgaagttttt
     3961 tcttttgatg aagtagcaga aacattggtt cttatccatg agatgcttga tggactgccg
     4021 ttattattta cgtttcgtac cttagctgaa ggcggagaac aagacatatc gatccgtcag
     4081 tatcgcgacc tttatgaaca tgtgatacac acaggattag tagatatggt cgatatcgag
     4141 ttgttcaaag tagaaagtct aggaaaaaga ctgatagagg aaatcaagtc tttaaacatt
     4201 cctttaatta tcagtagtca tgattttaaa gaaacaccag cagaccctgt cttgctttac
     4261 cgattgaatg ttatggaaca ttttggagcc gatatcggaa aactggcagt aactcccaat
     4321 aatgaacgag atgttttgcg gttgatggag ttgactagac gggcaaatgc ttttgtctcc
     4381 atgcctatta ttacgatgtc catgggaaat cttggtaaaa tttctcgtct ggctggcgga
     4441 acgacgggtt ccgttatgac atttggttcc ttaagcagcg attctacttc tgctccagga
     4501 caattaccgg tcaaagcatt gaagcaaatt attcaattat taaatgaacc gttgtagaac
     4561 aagtttgagg tgatattttt tggcaaagaa aaaaatagta aaaacgaatg caatccgtat
     4621 ggtcgaacaa aaaaagattc cctatacaga gcacgaatat gagtgggacg aaagccacct
     4681 aagtgcgtca tcggtcgctg aacagcttcc agaaagccaa tctcgtatat ttaaaacact
     4741 ggttgctgtg ggcaatgtga cggggcctct tgttgctgtg ataccgggag aagccgaatt
     4801 gaatctaaaa aaattagcaa aagtttcagg gaataaaaaa gtagaaatgc tccatttaaa
     4861 agatttggaa gcaacgaccg gatacatacg tggtggctgc tcgcctatag gtatgaaaaa
     4921 actatttcct acttatttag atcaaattgc agaaagctat gaacaaatca ttgtctcggc
     4981 tggcagaaga gggctacaga tggagttagc acctcaagat atctgtgcat taacgagcgg
     5041 acaatttgca gatataaaac aataaatcat acgtataaaa gaaagaagga ggccgggatg
     5101 ttgtttgtcc agtctccttc tttctttttt atacattatg ctctttgccc ggcattcatt
     5161 cctgctacat gccctgtagc gaaagccgct gtgatattaa agccgccagt ataaccattg
     5221 acatccagta attctcccgc aaaatacaga ccatcaatga ttttactctc caatgtttta
     5281 ggataaattt cctttagaga aacccctccg ccagttacaa atgatttctc aataggaaaa
     5341 gttttgttga ttgatagctt aaactctttg catagttgaa caaaatcttg aatttgtttt
     5401 tgagaggctt gttgtcctgt cagatgatcc atttcaagcc gctcaagaaa gaaaactaac
     5461 aatcgctcag gcaatatacc atgccaagca ttgaccaagt tctttttggt tgctttactt
     5521 ttttctgtca atatatgaat aagctcttgt ttggtttgtg tagggaaaca atccagagaa
     5581 agtgtcactg gttctcccgt tttctctaat tctttattga caaaacttga acaacgcagt
     5641 gcagccggac cagaaatacc gaaatgggtg aaaagtaaat ccatcgtatg ttcagtcaat
     5701 acacgacctt ttttatttaa aacgcgtaaa gtgatgtctt gtaaagaaag cccttgaagt
     5761 gttttctcct gaatatacgg ttcatccgat atcaaggggg actctgtcgc ataaagaggc
     5821 gtcaccgtgt gacccactcg cttcacgatc ttgtacccat ctccagtaga accagtagat
     5881 ggataagtcc gtccaccagt tgttaagata atggcaggag catggaactc ttcgaattcc
     5941 gtgcgtatac cgtaaatttt atgttctttg tggatcaatt tctctactcg agtagagaat
     6001 agaagcgtca catctagttc tttcagacga ttgagcaatg cttcgataat cgtttttgat
     6061 ttatttgtta caggaaacat ccgtccatga tcctcttctt ttaaatgtac gccttgtgat
     6121 tcaaagaagt tcatgatatc gaaattattc cattgcgcaa acgtactata taaaaacttt
     6181 ccatttccag gaatatgtgc aatcaaatca tcaacaggac gattgttcgt aacattgcac
     6241 cgaccaccac ctgtcatcag tagttttttt cctgcttttt tgtttttttc gatcaagagt
     6301 acgcgtgcgc cttgttcagc tgcactgatc gctgccatca tcccgcttgt tccagcgccg
     6361 actacgatca catcatacag tttttccatt ccctcacctc aaggaaagtg tagcatatct
     6421 gaagaaagaa aacagaaaaa aaatgttttg agccaaaaga gaaaatgagc taaaatgagg
     6481 gaagataagc aaaggagcgt ggaacgatga caaacgcaac agaatatgtt caagcaattc
     6541 aagaaaagct acatcaaaag gatcaagggc aagaagaatt tttacaagca atcgatgaat
     6601 tcatgcccac ggttatgtct tttttggaca ctcatcccga atatatcgaa aagaatatct
     6661 tagaactttt aacggaacca gaaagagtta ttcaatttcg agtgccttgg caagatgatg
     6721 aaggaaactg gcgagtaaat agagggtacc gtatccaata caactcagct atcggccctt
     6781 ataaaggtgg attgcgcttc catcctagtg tcaatctaag tatcttgaaa tttttggctt
     6841 ttgaacagat tttcaagaat agtttaaccg gtctgccaat cggtggagga aaaggcggaa
     6901 gtgacttcga tcctaaagga aaatctgata atgagatcat gcgtttttgt caaagtttca
     6961 tgctggaatt gtcaaaacat atcggcccat cgattgatgt tcctgcggga gatatcggtg
     7021 taggagctag agaaatcggc tatttgttcg gcgaatataa acgattgaaa aaatatgata
     7081 ctggtgtgtt gacaggtaaa cctttggatt tttggggaag taagatacgt acggaagcta
     7141 ctggttatgg attagtgtat tatgtcaaac atctattgaa cgaagaaaaa gattcattta
     7201 atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc catttatgca atcgaaaaag
     7261 tgcaagagct aggcgggaaa gttgttacat gttcagattc tagcgggtac atctatgatc
     7321 cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga aatcaataga caacgtttga
     7381 cggaatacgc aaaccaacgt ccttctgcta tttatcatgc tggagaatct gtttggacat
     7441 tgaaagaaaa agcggatatc gctttgccat gtgctactca aaatgaaata gacgaaagct
     7501 tggccgaaat cttagtagaa aatgggatca aaatcgtagc agaaggcgct aatatgccat
     7561 gtacagtaaa agctgtttct gttcttcatg aagcaggcgt gtggtattgt ccaggaaaag
     7621 ctgctaatgc tggaggagta gcagtatctg ctttagaaat gagtcagaac gtccagcgat
     7681 tgacttggga aagagagcaa gtggatgagc agctggatca aatcatggaa acgatttacc
     7741 atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa aaatctgctt cttggagcaa
     7801 atgttgctgg atttgaaaaa gttgccaaag caatggcagc agaaggttta gtttgaaaaa
     7861 tgaaactttc ttggaaatat tgtcagcatt ttgtttattt gcttgaaaaa taataaaaaa
     7921 aaacgtaaag tattacctgt agagtaaacc tataaggagg agaacaaatg gcacacattc
     7981 attttgacta ttcaaaagta gcaccgtttg tcaacgaaca agagttaggg tacatgcaaa
     8041 gccaagtaac agcagcccac aacgaattac gggaaggtac tggcgcagga agcgacttcc
     8101 gcggctggat cgatttacca acgaactatg acaaagaaga atttgcacgt atcaaagaag
     8161 cagctaaaaa aatccaatct gattctgaag tattagttgt tatcggtatc ggtggttctt
     8221 atttaggcgc tcgtgcagcg atcgacttct tgaatcacac attcttcaat ttattagata
     8281 gcaacacaag aaaagcacca caaatcttct ttgccggaaa ttcaatcagc tcaacatata
     8341 ttgctgactt gatcgaagta atcggtgatc gcgacttttc agtaaacgtt atttcaaaat
     8401 ctggaacaac tactgaacca gcgattgcgt tccgtgtatt caaagagctt cttgttaaga
     8461 aatatggaca agaagaagca aacaaacgta tctacgctac aacagataaa gcaaaaggtg
     8521 cagttaaagt agaagcagat gcagaaggtt gggaaacttt cgtgattcct gatgacatcg
     8581 gcggacgttt ctctgtatta actgctgttg gtttgttacc aatcgctgtt agtggtgcag
     8641 atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa agcatactca agcgacaaac
     8701 tagaagaaaa cgaagcttac caatacgcag caatgcgcaa cattctttac cgtaaaggca
     8761 aagtaactga gttgttgatc aactatgaac caggaatgca atacttctca gaatggtgga
     8821 aacaattgta tggtgaatct gaaggaaaag accaaaaagg catctatcct tcaagcgcaa
     8881 acttctcaac tgatttgcac tctttaggac aattcatcca agaaggacgt cgcaatattt
     8941 ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat cacaattcct gaacaagcag
     9001 aagatttaga cggtctaggc tacttgcaag gaaaagaagt ggacttcgta aatacaaaag
     9061 catttgaagg aacattgctt gctcatacag acggtgacgt gcctaacttg ctagtcaaaa
     9121 ttccagaaat ggatgcttat acattgggtt atacaatgta cttctttgaa attgctgtag
     9181 gtatttcagg ttacttgaat ggaatcaacc cattcgacca accaggtgta gaagcttaca
     9241 agaaaaacat gttcgcactt ctaggcaaac caggctttga agatcttgct aaagaattga
     9301 acgaacgcct gtaagataag ggtttttcgc tagatttaaa taatcagtga tagaaacatg
     9361 ttcgctttaa aatgaaaact ggtacccatt taggtactca aacatactgc atcgtttatt
     9421 cagaataagg ggctgtgaca agacgtttgt cacagtccct tgtttgtata gaaatcgatt
     9481 gattgccttg ctctttagtt tgttttagga ctttaagaac agtgaggctg acttcgaagg
     9541 ttaattctgt ttttttgttt tctagaatca gtaagcaaaa aagcactcac ttcgagtgct
     9601 tttttgcgat atattcttag cttaagatag gcctaatttt tcttttacag atgagggtac
     9661 cttatcgctg gtaattactt ttttctctat gatccgcttt tcgcttgctt tcaactgcaa
     9721 gatcgttcct tcggggtaag cagaaccatt ggcagagtca ccagaagtaa tgacatcgaa
     9781 atctaactgt cttttttcac cgttttcagc ataagcatct acgttgtatt taaaacctct
     9841 tccaattact tccccattat ctgctttgat atcagtttct gcaggcattg gagctttgac
     9901 taccgcataa taatctttac ctacataagt agactgatag tagtcccaac ctttccatcc
     9961 tataaaagca atcaatatga taacgatcaa ccctaaaatt tttttcatga aagatctcct
    10021 tttttatttt tattataccc gtggtgctag ttaattttaa tatcctaaag aatatctcaa
    10081 agttccttca aaacgtcgtt gtgctcgact taatagaaca ctgtaagcca gtaatatgct
    10141 ttcaaatcta ctctctagcc ctttaacaga acgtgagttg aaattttgac aacctaattt
    10201 ttctaaggaa gaaaaaacag tctcaactgt t
//
LOCUS       DACDKL010000088        10193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDKL010000088 DACDKL010000000
VERSION     DACDKL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.088"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            345..614
                     /gene="rpsN"
                     /locus_tag="IV329_001725"
     CDS             345..614
                     /gene="rpsN"
                     /locus_tag="IV329_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8940899.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            629..808
                     /gene="rpmF"
                     /locus_tag="IV329_001726"
     CDS             629..808
                     /gene="rpmF"
                     /locus_tag="IV329_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8940900.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            840..1217
                     /locus_tag="IV329_001727"
     CDS             840..1217
                     /locus_tag="IV329_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940901.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1238..1387
                     /gene="rpmG"
                     /locus_tag="IV329_001728"
     CDS             1238..1387
                     /gene="rpmG"
                     /locus_tag="IV329_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8940902.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1411..2394
                     /locus_tag="IV329_001729"
     CDS             1411..2394
                     /locus_tag="IV329_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8940903.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2424..2546
                     /locus_tag="IV329_001730"
     CDS             2424..2546
                     /locus_tag="IV329_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8940904.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2564..4087
                     /locus_tag="IV329_001731"
     CDS             2564..4087
                     /locus_tag="IV329_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8940905.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4111..4350
                     /locus_tag="IV329_001732"
     CDS             4111..4350
                     /locus_tag="IV329_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940906.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4543..5004)
                     /locus_tag="IV329_001733"
     CDS             complement(4543..5004)
                     /locus_tag="IV329_001733"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8940907.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5199..5417)
                     /locus_tag="IV329_001734"
     CDS             complement(5199..5417)
                     /locus_tag="IV329_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8940908.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5535..7151
                     /locus_tag="IV329_001735"
     CDS             5535..7151
                     /locus_tag="IV329_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8940909.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7392..8732)
                     /locus_tag="IV329_001736"
                     /pseudo
     CDS             complement(7392..8732)
                     /locus_tag="IV329_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9008..9163)
                     /locus_tag="IV329_001737"
     CDS             complement(9008..9163)
                     /locus_tag="IV329_001737"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8940910.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9459..9791)
                     /locus_tag="IV329_001738"
     CDS             complement(9459..9791)
                     /locus_tag="IV329_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8940911.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3392 a         1614 c         2013 g         3174 t
ORIGIN      
        1 ggcgataaac cagttaaaca taaaagattg aataaaaagt tatagtttct ttcatattgc
       61 cgttttaagt agaggaaaag ctacttaaaa cggtatttgt gtttgattgg actaataaaa
      121 gtgaattctt atttttcaga gcctaaactg catattcttt taaatgattt gattatctct
      181 atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta tttcatgtta
      241 tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta
      301 cgatttgagg tgtctaaatc aaaataaaag gagtataaag gtatatggca aaaaagtcga
      361 aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtatgcagat aaacgtcagg
      421 aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat tcaaatccga
      481 atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg cgaaagttcg
      541 gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct ggagtaaaaa
      601 aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa catctaaggc
      661 gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt catttgacga
      721 agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact ataatggaaa
      781 gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag ggaatcaaaa
      841 tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt gaaacgaccc
      901 agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga attattactg
      961 aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat tactatgagg
     1021 accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa gagtatgacg
     1081 aggagttatt tcaatgtatc gatggttatt gggagatacc tgttgatatg aaagaaaatt
     1141 tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa attcaagcat
     1201 atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata ttattttgga
     1261 atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga ataaccctga
     1321 acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt ttaaagaagt
     1381 gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat cgtttctggg
     1441 tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc accctacccc
     1501 agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta
     1561 ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt
     1621 cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa
     1681 ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa
     1741 acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt
     1801 attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc
     1861 atggcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa
     1921 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
     1981 gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc cttagttttt
     2041 gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca ccatcaagta
     2101 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttactc
     2161 caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
     2221 attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga acaggttaat
     2281 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg aaaaatgttg
     2341 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
     2401 gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
     2461 ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
     2521 gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
     2581 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
     2641 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
     2701 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
     2761 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
     2821 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
     2881 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
     2941 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
     3001 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
     3061 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
     3121 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
     3181 gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac aagttcctat
     3241 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
     3301 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaattgc
     3361 aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc ttgaaagtct
     3421 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
     3481 agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
     3541 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
     3601 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
     3661 tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg agtacaaaga
     3721 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
     3781 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
     3841 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
     3901 tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa gtaaggcaga
     3961 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg aattgcataa
     4021 ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag aaatgatggc
     4081 tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt gtctatggat
     4141 tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag attaaatggg
     4201 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
     4261 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
     4321 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
     4381 ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag cgcaacactt
     4441 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
     4501 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
     4561 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
     4621 aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga aatgattgga
     4681 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
     4741 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
     4801 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac cagttttgcc
     4861 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggacg
     4921 gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat tttttgtatg
     4981 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
     5041 ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac taattgaaaa
     5101 gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact gaaaatcgac
     5161 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
     5221 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt ttttttcttg
     5281 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
     5341 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag tatagtaaga
     5401 tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
     5461 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
     5521 gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat cttggttcag
     5581 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
     5641 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
     5701 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
     5761 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
     5821 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
     5881 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
     5941 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
     6001 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
     6061 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
     6121 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
     6181 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
     6241 cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
     6301 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
     6361 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
     6421 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat aaaagtattg
     6481 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
     6541 aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt aataaactag
     6601 tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag ggttgtttaa
     6661 ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg aaaaagtcta
     6721 atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc aagataggcg
     6781 atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt gtattctcta
     6841 tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt cgaattgatg
     6901 gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg aattatcatc
     6961 ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga gttaaagaag
     7021 ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga
     7081 gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc acagattact
     7141 acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat attattatta
     7201 ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg
     7261 acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag tcggttagat
     7321 tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga ataatcgaga
     7381 tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc tttagtcagt
     7441 tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat tcgttctcgg
     7501 tagttttgaa agtttctata gccataggca attcgtttta gtactttgat atggttattc
     7561 aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat ttctgtttgg
     7621 tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt gtagtcttct
     7681 tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag aaaatcctgg
     7741 tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc gactatctct
     7801 gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc tttcaattcc
     7861 cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg acgatcccct
     7921 tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc aatgtgttgg
     7981 ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt caccaatgtg
     8041 cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac ttttataggg
     8101 tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat atcaatcagt
     8161 tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt tttcactgat
     8221 ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat ggttttaggc
     8281 cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat acgtgcgatc
     8341 aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac tctgcgagca
     8401 atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc acagtgttta
     8461 catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac atcattcaat
     8521 aagatggata cttttttcca gccctattta atgatcgttt gttcattttg atagtggcga
     8581 tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aaacaaaaca tgtttcgtca
     8641 ttgattcgct ctttgcgaaa ggcatcttca tgaaagataa gatttaggtc tagaatatct
     8701 agtaactctt tggtaaactc tgaataggac atcttgaaac actcctttga atggttttcg
     8761 tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa aaagaaaaag
     8821 tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt ttcttcgctt
     8881 caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg tagtagtctt
     8941 ctaaataatc ttggattaac accttcatct ctattttact tttatatttg ttggagtcga
     9001 ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca acaggagtcc
     9061 cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga taaataaaag
     9121 accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct taataaattt
     9181 gtaatggacg gacgacaatc tcttttttca tgtgtgaatc taccatatag taacgtagaa
     9241 tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta catggaatgt
     9301 aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat cgattcaaca
     9361 tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc gtcgatgaaa
     9421 tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt tattgatttc
     9481 gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat ctttgtgttc
     9541 gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat gtagaaatct
     9601 gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta tcgacgatat
     9661 tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc aattttcttg
     9721 gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt acgatcatat
     9781 gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag aaaagtgtat
     9841 catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat agataagagt
     9901 ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg attggacaca
     9961 tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca attctagagg
    10021 agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc ccacttcata
    10081 cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa gctaaaaacg
    10141 aattaaaaaa cgttgataat taaaatcatt cgttttagat tctatgaatc att
//
LOCUS       DACDKL010000089        10184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDKL010000089 DACDKL010000000
VERSION     DACDKL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.089"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            109..615
                     /locus_tag="IV329_001739"
     CDS             109..615
                     /locus_tag="IV329_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8940912.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            632..1531
                     /locus_tag="IV329_001740"
     CDS             632..1531
                     /locus_tag="IV329_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8940913.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1618..2502
                     /locus_tag="IV329_001741"
     CDS             1618..2502
                     /locus_tag="IV329_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8940914.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2694..2765)
                     /locus_tag="IV329_001742"
     tRNA            complement(2694..2765)
                     /locus_tag="IV329_001742"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Arg,seq:ccg)
     gene            2825..4159
                     /gene="rpoN"
                     /locus_tag="IV329_001743"
     CDS             2825..4159
                     /gene="rpoN"
                     /locus_tag="IV329_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8940915.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4362..5399
                     /locus_tag="IV329_001744"
     CDS             4362..5399
                     /locus_tag="IV329_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8940916.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5440..6441
                     /gene="gap"
                     /locus_tag="IV329_001745"
     CDS             5440..6441
                     /gene="gap"
                     /locus_tag="IV329_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8940917.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6551..7741
                     /locus_tag="IV329_001746"
     CDS             6551..7741
                     /locus_tag="IV329_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8940918.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7845..8600
                     /locus_tag="IV329_001747"
     CDS             7845..8600
                     /locus_tag="IV329_001747"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8940919.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8716..10014
                     /gene="eno"
                     /locus_tag="IV329_001748"
     CDS             8716..10014
                     /gene="eno"
                     /locus_tag="IV329_001748"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8940920.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3564 a         1883 c         2111 g         2626 t
ORIGIN      
        1 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
       61 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
      121 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
      181 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
      241 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
      301 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
      361 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
      421 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
      481 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
      541 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
      601 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
      661 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
      721 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
      781 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
      841 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
      901 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
      961 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
     1021 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
     1081 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
     1141 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
     1201 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
     1261 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
     1321 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
     1381 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
     1441 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
     1501 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
     1561 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
     1621 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
     1681 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
     1741 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
     1801 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
     1861 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
     1921 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
     1981 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
     2041 cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc
     2101 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
     2161 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
     2221 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
     2281 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
     2341 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
     2401 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
     2461 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
     2521 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
     2581 caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc
     2641 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
     2701 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
     2761 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
     2821 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
     2881 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
     2941 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
     3001 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
     3061 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
     3121 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
     3181 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
     3241 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
     3301 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
     3361 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
     3421 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
     3481 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
     3541 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa
     3601 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
     3661 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg
     3721 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
     3781 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
     3841 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt
     3901 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
     3961 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
     4021 aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
     4081 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
     4141 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc
     4201 tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc
     4261 ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta
     4321 agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa
     4381 tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac
     4441 gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt atggggatca
     4501 cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg atcgacacct
     4561 caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact
     4621 tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta
     4681 accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat
     4741 tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag aatatcatcg
     4801 ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata
     4861 agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac
     4921 aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt
     4981 atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc
     5041 aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat
     5101 tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa
     5161 atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg
     5221 tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg
     5281 cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa
     5341 cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa
     5401 ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt
     5461 aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc
     5521 gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac
     5581 gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta
     5641 aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa
     5701 ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa
     5761 aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt
     5821 ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca
     5881 ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt
     5941 ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta
     6001 gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt
     6061 cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa
     6121 ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca
     6181 gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac
     6241 gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac
     6301 ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt
     6361 aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta
     6421 gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca
     6481 tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg
     6541 aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct
     6601 tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat
     6661 caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt
     6721 ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt
     6781 tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg
     6841 agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa
     6901 tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa
     6961 atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc
     7021 acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat
     7081 ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc
     7141 aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa
     7201 agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat
     7261 caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga
     7321 aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa
     7381 tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat
     7441 cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg
     7501 gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg
     7561 tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagccgc
     7621 agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag gtggcggcgc
     7681 aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata
     7741 aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat
     7801 gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta
     7861 tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag
     7921 taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat
     7981 tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact
     8041 gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc
     8101 taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag
     8161 acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat
     8221 gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac
     8281 aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt
     8341 atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa
     8401 tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag
     8461 ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag
     8521 aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca ttcttgtcat
     8581 tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact
     8641 aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca
     8701 aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca
     8761 cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga
     8821 atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac
     8881 aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt
     8941 gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc
     9001 gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct
     9061 atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc
     9121 ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat
     9181 gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc
     9241 aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct
     9301 cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac
     9361 gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa
     9421 gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt
     9481 tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac
     9541 gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga cgaaaacgac
     9601 tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac
     9661 gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac
     9721 tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa
     9781 atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat
     9841 tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac tggttctcta
     9901 tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt
     9961 gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt
    10021 tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt
    10081 aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg atgagttgtg
    10141 caaaatcaaa tctgagtcag aatgaaccac attctggctc agat
//
LOCUS       DACDKL010000090        10142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDKL010000090 DACDKL010000000
VERSION     DACDKL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.090"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..321
                     /locus_tag="IV329_001749"
     CDS             4..321
                     /locus_tag="IV329_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8940921.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            314..922
                     /locus_tag="IV329_001750"
     CDS             314..922
                     /locus_tag="IV329_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8940922.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            938..1768
                     /locus_tag="IV329_001751"
     CDS             938..1768
                     /locus_tag="IV329_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8940923.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            1906..2418
                     /locus_tag="IV329_001752"
     CDS             1906..2418
                     /locus_tag="IV329_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8940924.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            2444..3316
                     /locus_tag="IV329_001753"
     CDS             2444..3316
                     /locus_tag="IV329_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8940925.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3492..3854
                     /locus_tag="IV329_001754"
     CDS             3492..3854
                     /locus_tag="IV329_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8940926.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3863..4615
                     /locus_tag="IV329_001755"
     CDS             3863..4615
                     /locus_tag="IV329_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8940927.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4629..6431
                     /locus_tag="IV329_001756"
     CDS             4629..6431
                     /locus_tag="IV329_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940928.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6708..7748
                     /locus_tag="IV329_001757"
     CDS             6708..7748
                     /locus_tag="IV329_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8940929.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7800..9944)
                     /locus_tag="IV329_001758"
     CDS             complement(7800..9944)
                     /locus_tag="IV329_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8940930.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         3202 a         1739 c         2146 g         3055 t
ORIGIN      
        1 gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgctagc agcattaagc
       61 aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt
      121 gaatctacac tatatccggt tttgagaaga ttgaaaaaag aaggatatct tgagacctat
      181 gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt
      241 catttagaag aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg
      301 gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc
      361 tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa
      421 aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa
      481 aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag
      541 acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc
      601 actagcactg gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat
      661 ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc
      721 aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct
      781 gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct
      841 tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga
      901 tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat
      961 taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg
     1021 cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac
     1081 aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt
     1141 catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat
     1201 aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat
     1261 tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc
     1321 tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa
     1381 agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat
     1441 aactgcgaaa gatgaattag gctcacttcg attagatgag gccaatgtgg ataacttgat
     1501 cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa
     1561 agtcatccta aacggtggca atttatcttt gcatgagatg gaaaacagtg gctatactat
     1621 ccaaacgtct ggtgaatctt atattgaaaa agactaccag ccaataacaa atactttgtt
     1681 agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac
     1741 ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa
     1801 cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa
     1861 aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa
     1921 ggtcaaagag aaaagttaaa aacagaaatg gaaagattac atacttatat cactcaacta
     1981 agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca
     2041 gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag
     2101 aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg
     2161 aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg
     2221 gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa
     2281 ttggattatt taccgcaggc agccggaggg acacaggcaa ggggttggta catcactgaa
     2341 gacaaagagt tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaat taaaaaagta
     2401 acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag aaaaaagagc
     2461 attttatatc aatggttatg cagggattgt cggattggtc gtattagctt tagttggatt
     2521 gtttttcttc tatttaggta tgtggcaagc gaaagtttgg gcactttttt taagtatttt
     2581 tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc ctaatcaagc
     2641 aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg gctttttcct
     2701 tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt tcaatagttc
     2761 tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag tagtagtctt
     2821 taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag attttgtaga
     2881 aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg atacattcaa
     2941 tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt tgcaaaaaga
     3001 gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct tgaaccattt
     3061 agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa aagccatttt
     3121 gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg ccttagagca
     3181 aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat tgatcaataa
     3241 tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata caggcgacat
     3301 gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag gacggattat
     3361 tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa gaagaatgat
     3421 agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa agtaagagag
     3481 aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt gtggtacttg
     3541 caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat gtacaaatta
     3601 cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca gtcaactata
     3661 gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac ttcaagggac
     3721 aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac aaaaataaag
     3781 gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc gaaaacagca ctggaaaaac
     3841 taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg taacaaaagt
     3901 atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct tcgatgtaga
     3961 aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaagtcga cacttttgaa
     4021 tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc aaaaagatgt
     4081 gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg gctttatttt
     4141 ccaagatttc aatttattag aaagcttgac agcacaagaa aatatcgctg ttcctctttc
     4201 tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc caaaaaattg cagaacgttt
     4261 atccatctct catattttgg aaagctatcc ttctgagatt tccggtggac aaaaacaacg
     4321 ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg atgagcctac
     4381 aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg aattgaatac
     4441 aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa gttattgcca
     4501 acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg gagatcaaag
     4561 ccgtgattcc ttctatcgag aaatactatc tattcttgga aacttggaac agtaggggga
     4621 gattgactat gctttggaaa ttatcactta ctgggatcaa gagccgttta agagactact
     4681 tggtgttatt ctcaggatta gttatggcat ctgctatttt ttacatgttt gaatcaatgg
     4741 caagcaacga agcttttttg aaaagtaata cgatcgttgc ggctacagtt atcattttcc
     4801 gattcggttc tgttttacta gggatcatca cattcgttta tattttatat gcaaattcct
     4861 ttttgatgac gatgcggcaa aaagactatg caatgtttat gatgctggga gcaaaaggca
     4921 gaaaaatcgc acagatgatt ttttctgaaa cattcttagt agggatcgca gctactttag
     4981 tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca ccgattatta gtggatcagt
     5041 tagatctcaa tatttcgcat tttacacctt ttagtattca cgggttgttg atcactgttt
     5101 tgttttttgc tgttttgttt ttattagctg ctgttgtcaa tgctttttcg attgtcaaaa
     5161 aaccgatttt agcattgatt cgtgcggatc agacaccaac aagaatcaag cagcataaat
     5221 tcttgttttt ccttgaagta gtactgggaa ttgcttttct aggctgcggt tactatatga
     5281 tgaaacacgt ggggacctat caagtctttg gtataggggt agccttagtt acgatcatat
     5341 taggaactta tttcattttc cattctgtga tcattttctt tttatcatta ctaaagaaat
     5401 cagaacagat ttcattgaaa aaattgaaca atttcacatt gtcacagtta aactttcgta
     5461 ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct gtttgccttg gcactgggcg
     5521 ctttgacggt aggattaggt tttaaaaatg aaattgcaga cttgacagaa aacaattctg
     5581 gatatgatct agtgatgaat aatcctcggg cagaagatca gcaaaaaatc aaagaacttt
     5641 caccaacact ttctgcttct tatactcaaa aagaagatgc gggaacgatt tactataatg
     5701 caagtgaatt tgcggctcaa ccattgatca tgataaaaca tgaagatgga acaccgccga
     5761 agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga actgactgta caagaggaac
     5821 ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat caaggtagtc agtcaatcag
     5881 aatttgatca actgaacttg ccacaatcca ctttacagct agttcaggta aaagacttcc
     5941 aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa taaaatgaac aatccatctt
     6001 tgcaaaaagt agagtacagc aaccagaaat atgaaatgta tgaagtgtat aatggtctat
     6061 tctctggttt tgaatttatg ggattcttcc taggtttagc tttcttgacc atgcttgcta
     6121 gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga cattgctcgt tatgaaatgc
     6181 ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc gatccgtaga gaagtaggcg
     6241 tgttattctt agcaccagga atcttaggtg tagtccatgt cttattcgga ttacaaatgt
     6301 ttggtctatt catgcaaaat ccatacaaag atatttgggt tccgtttacg atcttcttcg
     6361 tattgtattt catctactat gtactgacga catggttata tactggaatc gtattgaaaa
     6421 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaagca aaaatagctc
     6481 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgtttg
     6541 ttcggaaaaa gaggttgtga caatattttt gtcacaacct ttttttcacg gtcttttatt
     6601 agaatatagt aaattttttc tcgttactca cggaataagc tgttaagaaa acggtttcca
     6661 tgtatactgg aatcagtgca aatgaaagaa aagagggtgc aaggaacatg gaaatcaatg
     6721 aacaaaattt tggcgaaaac tatcacttga ttacgatcac gaatcaaaat gggacatctt
     6781 tgtctgtcag tgatcttggg gcacgggttg ttcattttca aacaataatc gataatgaaa
     6841 aacgagagtt gatcttaggt tttgatgaag caaaagaata tctagaaaaa gacccttata
     6901 tcggtgcttc aatcggacgt atggctggac ggattacgaa tggcacattc aaattagatg
     6961 atgaaacgtt cgaagtaaaa acagacccgg acactggtca ttgtttacat ggtggtgcac
     7021 ctagctttga agccaagcag tggcattata cagtcatcaa tggtgaaaac gaagcatcgg
     7081 tcattttcta tcagactagc ccagatatgg gaaacggatt tcctggaact ttagatgtag
     7141 aagtacggta tacgctgacg aacgatgata catggcgagt gacggtccaa gggatcagtg
     7201 accgcaaaac agtattcaat cctacaaatc atgtctattt caatttaacc ggtgatccag
     7261 cacagtcaat cgacgaacat atactttggt tgaatagccg attatttgct acgctcaata
     7321 aagatacaac gcctacagga gaagtagttt cagtaagtgg cacagcattt gacttccaac
     7381 gtcctaaaaa attaggtgag gtctttgcca gtgattttcg ccaatcaatg atagtgggcg
     7441 gcatcgatca tccatttttg ttgaaaaaac aatctggttt actaaatgct gcggaattga
     7501 caagcccaga tgaaaaagta tcgatccaag taaaaacaga tgcttcagga atcgtgattt
     7561 ttacagcaaa ttttggagaa gatggaccgg aaatgcgcgg gaataaatta gtaaatcatg
     7621 gcggtatcac atttgagact caagaactgc caggagcgga gcgacttgaa agttttggag
     7681 atgtcactct ggaacctaac caagtacgcg agtttgtaac ggaatacaaa ataaatataa
     7741 agaaatagaa taaattaaaa agggagtgtg acagtgtcac atcccctttt taatttattc
     7801 tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa ggttagtaca
     7861 ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct tgccgtaaaa
     7921 ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt gttgacgcgt
     7981 tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca tttgattggc
     8041 acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt tgttgctgct
     8101 tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc gttcaatttt
     8161 tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc ttctttgtag
     8221 tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt cagtccattt
     8281 ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt acggaattgt
     8341 gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa ggtcacgctg
     8401 cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct tgataataag
     8461 ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc gtagattcct
     8521 tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg atcagttgtt
     8581 tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga gccgcctgtc
     8641 agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa atcaggcgtg
     8701 aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg gataaattgt
     8761 ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc ccagaagacg
     8821 agatcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg ctgttcgcct
     8881 gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt caaaccagaa
     8941 cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag atgcttgaat
     9001 gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac agcatcaatt
     9061 ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc gctttgattt
     9121 gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc attgtttgga
     9181 tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt tgtttgattc
     9241 gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga accattatta
     9301 ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt actagggact
     9361 tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt aaattctgca
     9421 gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt tacttctttg
     9481 gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat agtgtaagtt
     9541 accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc caaaggttgc
     9601 tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa agaaggagca
     9661 attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc cggatcaatt
     9721 tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc ttctgtaaaa
     9781 gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt gactgtttga
     9841 tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga cggagcaatc
     9901 agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa ctagtccttt
     9961 tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg aacagataat
    10021 cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata aaacaatttg
    10081 taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg catagaccac
    10141 ct
//
LOCUS       DACDKL010000091        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDKL010000091 DACDKL010000000
VERSION     DACDKL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.091"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IV329_001759"
     CDS             complement(247..759)
                     /locus_tag="IV329_001759"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8940931.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV329_001760"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IV329_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8940932.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IV329_001761"
     CDS             complement(3212..3646)
                     /locus_tag="IV329_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8940933.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IV329_001762"
     CDS             complement(3656..4444)
                     /locus_tag="IV329_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8940934.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IV329_001763"
     CDS             complement(4441..5382)
                     /locus_tag="IV329_001763"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8940935.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IV329_001764"
     CDS             complement(5559..6146)
                     /locus_tag="IV329_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8940936.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV329_001765"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IV329_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8940937.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IV329_001766"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IV329_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8940938.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IV329_001767"
     CDS             complement(9063..9998)
                     /locus_tag="IV329_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8940939.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDKL010000092        10010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDKL010000092 DACDKL010000000
VERSION     DACDKL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.092"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            27..176
                     /locus_tag="IV329_001768"
     CDS             27..176
                     /locus_tag="IV329_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8940940.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            187..1701
                     /gene="dltA"
                     /locus_tag="IV329_001769"
     CDS             187..1701
                     /gene="dltA"
                     /locus_tag="IV329_001769"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8940941.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1698..2903
                     /gene="dltB"
                     /locus_tag="IV329_001770"
     CDS             1698..2903
                     /gene="dltB"
                     /locus_tag="IV329_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8940942.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            2948..3181
                     /gene="dltC"
                     /locus_tag="IV329_001771"
     CDS             2948..3181
                     /gene="dltC"
                     /locus_tag="IV329_001771"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8940943.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3184..4452
                     /gene="dltD"
                     /locus_tag="IV329_001772"
     CDS             3184..4452
                     /gene="dltD"
                     /locus_tag="IV329_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8940944.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4593..5675
                     /locus_tag="IV329_001773"
     CDS             4593..5675
                     /locus_tag="IV329_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8940945.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5675..6478
                     /locus_tag="IV329_001774"
     CDS             5675..6478
                     /locus_tag="IV329_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8940946.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6548..7288
                     /locus_tag="IV329_001775"
     CDS             6548..7288
                     /locus_tag="IV329_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8940947.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7299..8303
                     /locus_tag="IV329_001776"
     CDS             7299..8303
                     /locus_tag="IV329_001776"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8940948.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8348..>10010)
                     /locus_tag="IV329_001777"
     CDS             complement(8348..>10010)
                     /locus_tag="IV329_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940949.1"
                     /translation="KDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKG
                     NHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGL
                     PPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILL
                     SCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYG
                     IFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYV
                     ACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKD
                     RRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVE
                     KILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQ
                     LLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEEL
                     DDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3197 a         1667 c         2044 g         3102 t
ORIGIN      
        1 gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt
       61 attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt
      121 atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg
      181 aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct
      241 gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca
      301 gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt
      361 ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc
      421 tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct
      481 aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc
      541 ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat
      601 agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca
      661 aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac
      721 tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg
      781 gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca
      841 gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg
      901 gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat
      961 gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa
     1021 agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca
     1081 actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta
     1141 ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt
     1201 tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg
     1261 aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat
     1321 acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat
     1381 cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc
     1441 caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag
     1501 cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact
     1561 aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc
     1621 cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca
     1681 gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt
     1741 tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa
     1801 gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta
     1861 attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat
     1921 ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta
     1981 ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag
     2041 catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca
     2101 tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc
     2161 tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc
     2221 agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata
     2281 tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc
     2341 ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata
     2401 tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg
     2461 gtacaagcta catgatggga tatcagacac caatcaattt caataaacct tttttatcat
     2521 ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt
     2581 atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg
     2641 taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga
     2701 cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat
     2761 ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct
     2821 tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt
     2881 tagataaact tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa
     2941 aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt
     3001 aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt
     3061 tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga
     3121 gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg
     3181 agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg
     3241 tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt
     3301 gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta
     3361 tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt
     3421 catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca
     3481 cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa
     3541 aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact
     3601 gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag
     3661 aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa
     3721 gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct
     3781 gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc
     3841 agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca
     3901 aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg
     3961 acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag
     4021 ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat
     4081 tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca
     4141 ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt
     4201 gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc
     4261 aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg
     4321 ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag
     4381 tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta
     4441 caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt
     4501 ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat
     4561 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
     4621 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
     4681 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
     4741 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
     4801 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
     4861 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
     4921 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
     4981 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
     5041 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
     5101 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
     5161 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
     5221 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
     5281 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
     5341 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
     5401 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
     5461 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
     5521 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
     5581 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
     5641 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
     5701 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
     5761 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
     5821 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
     5881 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
     5941 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
     6001 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
     6061 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
     6121 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
     6181 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
     6241 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
     6301 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
     6361 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
     6421 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
     6481 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
     6541 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
     6601 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
     6661 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
     6721 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
     6781 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
     6841 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
     6901 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
     6961 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
     7021 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
     7081 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
     7141 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
     7201 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
     7261 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
     7321 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
     7381 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
     7441 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
     7501 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
     7561 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
     7621 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
     7681 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
     7741 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
     7801 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
     7861 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
     7921 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
     7981 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
     8041 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
     8101 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
     8161 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
     8221 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
     8281 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
     8341 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
     8401 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
     8461 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
     8521 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
     8581 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
     8641 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
     8701 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
     8761 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
     8821 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
     8881 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
     8941 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
     9001 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
     9061 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
     9121 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
     9181 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
     9241 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
     9301 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
     9361 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
     9421 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
     9481 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
     9541 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
     9601 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
     9661 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
     9721 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
     9781 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
     9841 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
     9901 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
     9961 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt
//
LOCUS       DACDKL010000093         9686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDKL010000093 DACDKL010000000
VERSION     DACDKL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.093"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..636
                     /locus_tag="IV329_001778"
     CDS             13..636
                     /locus_tag="IV329_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940950.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /locus_tag="IV329_001779"
     CDS             1086..2135
                     /locus_tag="IV329_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8940951.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="IV329_001780"
     CDS             2132..3013
                     /locus_tag="IV329_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8940952.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="IV329_001781"
     CDS             3016..3786
                     /locus_tag="IV329_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8940953.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="IV329_001782"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="IV329_001782"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8940954.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="IV329_001783"
     CDS             5097..6260
                     /locus_tag="IV329_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8940955.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="IV329_001784"
     CDS             6377..6787
                     /locus_tag="IV329_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8940956.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="IV329_001785"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="IV329_001785"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8940957.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9327..9644
                     /locus_tag="IV329_001786"
     CDS             9327..9644
                     /locus_tag="IV329_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940958.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
BASE COUNT         3356 a         1645 c         2083 g         2602 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa cttttt
//
LOCUS       DACDKL010000094         9683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDKL010000094 DACDKL010000000
VERSION     DACDKL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.094"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1105)
                     /locus_tag="IV329_001787"
     CDS             complement(<1..1105)
                     /locus_tag="IV329_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP8940959.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYN"
     gene            complement(1977..3608)
                     /locus_tag="IV329_001788"
     CDS             complement(1977..3608)
                     /locus_tag="IV329_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8940960.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3622..5295)
                     /locus_tag="IV329_001789"
     CDS             complement(3622..5295)
                     /locus_tag="IV329_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8940961.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5308..5625)
                     /locus_tag="IV329_001790"
     CDS             complement(5308..5625)
                     /locus_tag="IV329_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8940962.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5650..7050)
                     /gene="lacG"
                     /locus_tag="IV329_001791"
     CDS             complement(5650..7050)
                     /gene="lacG"
                     /locus_tag="IV329_001791"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8940963.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7287..8072
                     /locus_tag="IV329_001792"
     CDS             7287..8072
                     /locus_tag="IV329_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8940964.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8075..8767
                     /locus_tag="IV329_001793"
     CDS             8075..8767
                     /locus_tag="IV329_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8940965.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9041..9412)
                     /locus_tag="IV329_001794"
     CDS             complement(9041..9412)
                     /locus_tag="IV329_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8940966.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2941 a         2110 c         1690 g         2942 t
ORIGIN      
        1 ggttgtaagt atcaaaagac gagactaaga aacgttccat cgactcttca ttttggaact
       61 gttccttgcg gtggctgtat ttcttgattt gtttattaaa ggattcaatc aagttcgtag
      121 aataaatact cttgcgtatg gccaagggga aatcatagaa agtgagcaag tgatcgttcg
      181 aaagaatcga ttcaaccact tttggatagc tggttttcca tttttccgca aaagtaccac
      241 gtgcttccaa cgccacctct ttagatgagg cttgatacac cattttaaaa tcatcacaga
      301 cttccttacg atcatcgaca cgcactttat gactgatatt acgggaaacg tgtacacaac
      361 aatgttgaaa acgagctttg ggatagaacc gactaatcgc tcctaccatc ccctttaagc
      421 catccgtgat gaacaggagg acatttttca aaccgcgctc ttgaaggtcc aataaaattt
      481 cctcccaaat cgtgatggat tcagtcggtg caatcgcata gctcaatact tcctttgatc
      541 cgtccgggcg aatgccaact gcgatatgaa tagcttcctt ggcgacggtt ttccgcttta
      601 acggaatata cgttgcgtcc atataaatag cagcataacg gtcatgaagc tcccgcccat
      661 taaacgccgt tacctcttct gtaaatgatt ttgttatatt ggacatggtt tggggcgtgt
      721 agtaatgccc atacattttc tcaatcaagt ctgcgatttc cgacatggta ataccttttc
      781 ggaagaggtg aatgacggtc tcctctaacg tgtcattcgt ccgtctataa gcaggaacag
      841 tctgttgctt gaactcacca ttgcggtcgc gcggaatctg gagatgaagt tccccatact
      901 cggtcttgac cgtacggtca taggagccgt tacgagaatt acccgtgtta aaaccaatgc
      961 gatcatactt ttcgtaatct aggaaagccg ttaactccgt tttgagaagc gtattgactg
     1021 ctttctccaa atggacacgg aataattcat tcaaatcgcc tttgttcgct agagtcttta
     1081 gaatttctgt agtaaaatcg ttcataggga agtcctctct tctgtgaatg tgttgtggta
     1141 actctattct acagaaggga cttccttttt tctatttaca caaaatattt tacactctcc
     1201 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
     1261 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
     1321 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
     1381 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
     1441 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
     1501 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
     1561 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
     1621 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
     1681 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
     1741 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
     1801 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
     1861 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
     1921 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
     1981 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
     2041 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
     2101 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
     2161 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     2221 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     2281 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     2341 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     2401 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     2461 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     2521 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     2581 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     2641 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     2701 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     2761 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     2821 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     2881 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     2941 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     3001 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     3061 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     3121 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     3181 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     3241 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     3301 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     3361 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     3421 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     3481 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     3541 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     3601 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     3661 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     3721 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     3781 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     3841 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     3901 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     3961 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     4021 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     4081 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     4141 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     4201 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     4261 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     4321 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     4381 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     4441 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     4501 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     4561 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     4621 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     4681 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     4741 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     4801 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     4861 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     4921 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     4981 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     5041 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     5101 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     5161 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     5221 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     5281 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     5341 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     5401 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     5461 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     5521 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     5581 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     5641 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     5701 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     5761 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     5821 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     5881 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     5941 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     6001 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     6061 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     6121 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     6181 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     6241 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     6301 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     6361 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     6421 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     6481 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     6541 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     6601 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     6661 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     6721 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     6781 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     6841 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     6901 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     6961 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     7021 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     7081 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     7141 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     7201 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     7261 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     7321 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     7381 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     7441 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     7501 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     7561 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     7621 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     7681 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     7741 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     7801 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     7861 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     7921 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     7981 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     8041 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     8101 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     8161 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     8221 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     8281 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     8341 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     8401 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     8461 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     8521 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     8581 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     8641 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     8701 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     8761 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     8821 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     8881 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     8941 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     9001 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     9061 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     9121 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     9181 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     9241 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     9301 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     9361 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     9421 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     9481 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     9541 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     9601 caataagcta attaagaaac aaactttaga aactctagaa gaacttctga atttatatag
     9661 aagtgtgtag ctacttacta aat
//
LOCUS       DACDKL010000095         9634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDKL010000095 DACDKL010000000
VERSION     DACDKL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.095"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..533
                     /locus_tag="IV329_001795"
     CDS             54..533
                     /locus_tag="IV329_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940967.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            566..2794
                     /locus_tag="IV329_001796"
     CDS             566..2794
                     /locus_tag="IV329_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8940968.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            2923..4566
                     /locus_tag="IV329_001797"
     CDS             2923..4566
                     /locus_tag="IV329_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8940969.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4639..5184)
                     /locus_tag="IV329_001798"
     CDS             complement(4639..5184)
                     /locus_tag="IV329_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8940970.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5207..5692)
                     /locus_tag="IV329_001799"
     CDS             complement(5207..5692)
                     /locus_tag="IV329_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8940971.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5682..6494)
                     /locus_tag="IV329_001800"
     CDS             complement(5682..6494)
                     /locus_tag="IV329_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8940972.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6633..8165)
                     /locus_tag="IV329_001801"
     CDS             complement(6633..8165)
                     /locus_tag="IV329_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8940973.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8274..9305)
                     /locus_tag="IV329_001802"
     CDS             complement(8274..9305)
                     /locus_tag="IV329_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8940974.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3212 a         1798 c         1860 g         2764 t
ORIGIN      
        1 taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa
       61 taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg
      121 gaagaaatgc attaaatatg gatgcaaaag ggctttcact gggccttgct cagtggaatg
      181 atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac
      241 gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta gctgcgcaag
      301 gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag
      361 tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat accaaaaatc
      421 caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga
      481 ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag
      541 agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt
      601 ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga
      661 agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag
      721 taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc
      781 tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga
      841 tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact
      901 agaagctatt taccaaacag atacacttga agctgtactg acactgatcc agaaccatta
      961 cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc
     1021 tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc gagcgattga
     1081 aaaaggctat ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt
     1141 ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg
     1201 tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgccc
     1261 attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt
     1321 agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag aacttctctt
     1381 aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg
     1441 tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt
     1501 actccagtcg atttatccaa aagttttgta taaattatcc actgattcag atgaattggc
     1561 aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag
     1621 aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa gaaagaacca ttagcgccga
     1681 agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa
     1741 gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga
     1801 aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa
     1861 tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc
     1921 acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac
     1981 gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa aaacgccgga
     2041 agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt
     2101 agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg
     2161 gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa agaacccgcc
     2221 acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt caatgatacg
     2281 ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat tttctaagaa
     2341 agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa gagaaaagac
     2401 ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg aaaaattagt
     2461 cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag cagcagaaaa
     2521 attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg gtcagccaaa
     2581 agcattgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga
     2641 atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca aagaagaaat
     2701 caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg aatttcccaa
     2761 gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac gaacgcactt
     2821 agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt ctaaaataga
     2881 atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca agaacaaaaa
     2941 tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc
     3001 gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa agatcttggt
     3061 atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa tggttatgac
     3121 gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt tgatttattg
     3181 ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat caaccatagt
     3241 tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat
     3301 tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac atcgattttc
     3361 ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca tgtttttgca
     3421 aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact attcaatatg
     3481 atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat ttctcatgta
     3541 aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc
     3601 tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta cgatatcatg
     3661 actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt agggaaagac
     3721 ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca agaaaaagaa
     3781 gggcaattag atgtccttaa attgaaacat gcattaagcg cttggcaaac ttcattggat
     3841 ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg
     3901 tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc tatgatgtat
     3961 ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat gacgaatatg
     4021 ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta taaaaggctg
     4081 cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac aacgagagac
     4141 aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc tacacatgaa
     4201 ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca agaatatgaa
     4261 ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac caataaaggt
     4321 ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta tgtctatgaa
     4381 agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc acttgctgac
     4441 tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa ttcctctagc
     4501 ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata taaaacaaac
     4561 aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt tttcagattg
     4621 ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt taggacgaga
     4681 gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga caggtacttg
     4741 gacatgcttg atatgcatac cgattgatgt gtcaccgata tcgataccgg cttgtccaac
     4801 tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc
     4861 tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt ctgccactgt
     4921 tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta gcgcaatttc
     4981 tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt ccgcactgga
     5041 attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa caaaaagatc
     5101 accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca attggtcttt
     5161 taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa tattgcttcc
     5221 gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt ccaatcagat
     5281 tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat aaaatccaac
     5341 cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct aatccccaca
     5401 tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg tggatcacta
     5461 atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga ccactggaag
     5521 caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag atcccggcct
     5581 cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt aatgaaaggg
     5641 caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc atttctcatt
     5701 cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat cagcacgcca
     5761 ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt aaaatctgcc
     5821 cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc gtttactgtt
     5881 gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata gagaagatcc
     5941 acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt tgttcctgaa
     6001 gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag agcttgacca
     6061 gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg aaaaagttct
     6121 tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt gaatgccaaa
     6181 acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg aacaaccatg
     6241 attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt cattttatca
     6301 atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat aaatcgctca
     6361 ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc aaaattctca
     6421 aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc gccaattgtc
     6481 aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt ttcaaaaaag
     6541 aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa aagaaaagaa
     6601 agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc
     6661 tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg
     6721 atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc
     6781 atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca
     6841 atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta
     6901 ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta
     6961 tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt
     7021 gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct
     7081 gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc
     7141 agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta
     7201 agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc
     7261 atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga
     7321 tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca
     7381 aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact
     7441 tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag
     7501 ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc
     7561 gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa
     7621 tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca
     7681 tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata
     7741 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
     7801 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
     7861 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
     7921 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
     7981 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
     8041 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
     8101 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
     8161 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
     8221 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
     8281 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
     8341 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
     8401 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
     8461 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
     8521 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
     8581 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
     8641 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
     8701 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     8761 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     8821 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     8881 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     8941 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     9001 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     9061 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     9121 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     9181 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     9241 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     9301 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     9361 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     9421 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     9481 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     9541 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     9601 tcagaatcga ttggctctcc atttattcaa taat
//
LOCUS       DACDKL010000096         9401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDKL010000096 DACDKL010000000
VERSION     DACDKL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.096"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            289..3057
                     /locus_tag="IV329_001803"
     CDS             289..3057
                     /locus_tag="IV329_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8940975.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3138..3557
                     /locus_tag="IV329_001804"
     CDS             3138..3557
                     /locus_tag="IV329_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8940976.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3569..4039
                     /locus_tag="IV329_001805"
     CDS             3569..4039
                     /locus_tag="IV329_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8940977.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4056..4835
                     /locus_tag="IV329_001806"
     CDS             4056..4835
                     /locus_tag="IV329_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8940978.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4822..5643
                     /locus_tag="IV329_001807"
     CDS             4822..5643
                     /locus_tag="IV329_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8940979.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5660..6709
                     /locus_tag="IV329_001808"
     CDS             5660..6709
                     /locus_tag="IV329_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8940980.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6722..7726
                     /locus_tag="IV329_001809"
     CDS             6722..7726
                     /locus_tag="IV329_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8940981.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8359..9378
                     /locus_tag="IV329_001810"
     CDS             8359..9378
                     /locus_tag="IV329_001810"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8940982.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3131 a         1568 c         1978 g         2724 t
ORIGIN      
        1 aatacaacta cagcgagata agatccgatg atttctcgaa aagttttcca ggaagtatgt
       61 ttcctgtttt tatatttatc cgatttcatt gaatagtcat agcctcctta ttctttcata
      121 tgttgaaatt ccagtataaa aactggaagg aactgtacgt tttttagaat aaaaattata
      181 agggataagt aggctgctgt ctataataag atatattttt caattcttta ttaaagattt
      241 tattgacaaa atgaatccgt tttctatgat ggaggagggg tgaataagtt gttgaagaag
      301 gaaattttat gttacttaga gaaccagaca gcctttttcg atttagaaca tatgaacgag
      361 gtctttacag cacaaaggtt agctgatcat tttggggtaa aaagaaacac agtcagtcat
      421 tatttgaacc agttaaatga tgagcaacta ctagtcaaaa tcaattctcg accagtgatt
      481 tatttccaca aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt
      541 tctatcgatg atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga cttattctct
      601 tttatgatcg gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt
      661 tactatccag atggcggatt accacttttg attacaggcg aaagtggcac aggtaaaagt
      721 tatttagtta atttggtcta tcagtattgt ctgcttcatg atctgctaga agattctgca
      781 ccattcatca cttttaattg tgcacaatat gcagataatc ctgaactttt gactagcaat
      841 ttgtttggac atgttaaggg agcgtacact ggtgcagaag aaaataaaaa aggggctttt
      901 gaagcagcag atggtgggat cttgttttta gacgaggtac atcgcttaac accagaaggt
      961 caggaaaaac tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg agaacctaat
     1021 ttgtcacgcc ggatcaaaac acgtctcttt tttgctacga ctgaagagat tactagtcat
     1081 ttcttaacaa ctttcattcg aagaatacct atccgaatcg aactacctgc acttaatcag
     1141 cgtagtcgta atgaacggct agaactgatt tactcttttt ttctcgaaga acaaagaaaa
     1201 atggggagcc ctttgtcagt cagtggacca gctttgtcac ttttaacaag tcagaaatta
     1261 tccggaaaca taggagagct gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct
     1321 gaacagcgtg aacagcctga aatccatgtc acgatccatc atttgcaaaa agaactactt
     1381 gcgcagccta ttactaaaaa tacagcacag caaggaatct atattacgca agaccagacc
     1441 ttagagcaat taatggagaa gcaacagccc gaacagcagc gaatcgttaa aagttttgag
     1501 caaatcctaa ttgatttcaa gaaaaataac tgtctgctat attcttgcga aggaaaatta
     1561 aagcaagaag tgattcaatt atttgatttt ttgctgtttg agaccagtag acaagaaaaa
     1621 cacgagctgc tggtttattg gacacaatct atccgagaga cctttcgaca aatggaaact
     1681 gcctatcaaa tcaaattcga tggaaacagt gtctatgcct tgagttacta tctctatcag
     1741 cgaagcacta tcaaatggtt accggaagaa catgatatta tccaactaat caaagaatta
     1801 gaacaacaag tttctcagtc ttatccatct agttatcact atgtccaacg aattctggaa
     1861 ttaagtaaga tcaaattaga tattgaagac acaagtatgg atcggatttt actgacgatc
     1921 tatctaaaaa aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat gattgctgct
     1981 cacggttatg caactgctag cagtatggca aatgtagtaa atcgtttatt acaagaggat
     2041 ctatttgaat catttgatat gccactagat gtcacaccac agcagattgc ttcagaaatc
     2101 cttgattatt gcgaaaacag cgatgtttca aatggattga tcatattagt agacatggga
     2161 tctctgaaag aaatccatca gttttttaaa aaacagttat ccgtaccgct tttgattttg
     2221 aataatgtga cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca
     2281 ttagaagaga tagcagaaga gactgttcga cagatccagc cagaatggcg actgctctat
     2341 ccagaggaaa acaaacccaa agcactcatt actacttgct tcactggaat cggtacggcg
     2401 gtccacttaa gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc
     2461 ccatatgaat atcagcaatt aaaagataag aaaaacagtg acccattatt ttccatatac
     2521 gaagtcgtag gaatgattgg caccacagat cctgatatca ccaatatcga ctatctttca
     2581 ttagaagacc tcatctcggg ggaaaagatg tctgtactag ctgaatggtt ggacactact
     2641 atgaatgctt cagagaagga aatattcaat cagcgcatca ttcgtaattt ttcattggaa
     2701 aaagtactgg attccgtgac tattctagat acagagaagg tgatgggaga aatcgatcat
     2761 tttatgagag aattagaggt gcagttgaat cgaagcatca gcaatcccaa aaaattagcg
     2821 ttgtatgtcc atgtgagctg tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc
     2881 tatcaaggat tagagaaatg gaagcagtgt caaaaagaag ggttatctgc tattaaatct
     2941 gcgtttagtg tcattgaaga gcattatagt gtcatgatcc ccaattctga gattgcctat
     3001 atctatgata ttttgagtga agatacagag tttctctcga tagaagaaga attttgaaca
     3061 taagcaagtt gttgccacta attttggcat gcaacttgct tttttagtat gtgtaagtga
     3121 aaagaggaga tgaaaaagtg gatagagaaa ttatcttagc ttcccatgga aaatttgctt
     3181 cagggatatt agattcatta gagctgatct atgggaaaaa tcattctatt actgtattcg
     3241 attgctatac tgatgagaat tttgatttag cggaaacagt aaggcagctt tttatgaggt
     3301 acaaagataa agaaatcatt gttctgacgg atctttttgg cggaagtgtc aacaacgaat
     3361 tcttacaata tatcaatcgg gatcatgtct atctcgttgc cggattgaat ttgccactat
     3421 tgattgagct agtatctcgg ttggacgctg aagtagagac aactgttttg atccagcagg
     3481 tgctaactga ttcaaaaaaa atgatccagt tttgtaacga gcggctcaat cagaaaatag
     3541 aagaagacga attttaggag gaagaaaaat gattgtattg acaagagtcg accatcggtt
     3601 attacacgga caagtagctt tttcatggac acaaacaata ggcgcagact gtattttgat
     3661 tgctaatgat gatgtaccta cgaatgagat acgaaagaca acgatcaaat tagccaagcc
     3721 acaaggagtc aaactagtga tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg
     3781 ggtgacagat aaatataagt tattcatcgt agtggaatcg atcgaggatg catataagct
     3841 ggcagaagcg tatccagaaa tcaaaaccat caacttagga gggatcaagg caaaacctgg
     3901 tactcgcagc atcggaaaag cggtaaatat tttaccagaa gaggaggaac gcttgaaacg
     3961 attaattgac aaacaaatag aagtagaaat ccgtcaagta ccagcagata aaaaagtgga
     4021 tgtaagagaa gttttgtaag aaaggaagaa cctcaatgat tgtacaagca gtcttattag
     4081 gaattgttgc atttgtcgca caatcagaat atgcactagg gacatcgttg ctttctcgtc
     4141 cgattgtgac ggggctgttt acaggaatcg tgttaggtga tgtaaaaact gggatcatta
     4201 tgggcgcaac tttagagtta gcatttatcg gatcgttctc agtaggtgct tcgattccac
     4261 ctgatgttgt gaccggtggg attttaggga cggcttttgc tattacagct ggagccggta
     4321 cagaaaccgc gcttttacta ggtttgccga ttgctacgtt gacattgata ttgaaaaata
     4381 tttacttagg gcttttgatt ccaattatga accataaagc agacaattat gcagaagaag
     4441 gaaattataa aggagtcgag catatgcact tgcttgccgg tatcggtctt tccttgatgc
     4501 ttggtgttgt ggtaatggtt tcttatatgg tgggaagcaa tacgatcgga aatctattaa
     4561 atatgattcc tgacttcgtt caaaaggggt tgtcagtagc tacagggttg attccagctc
     4621 ttggttttgc gatgcttgct cgtttgctga tcaacaaaca agttgcacca tattttttct
     4681 taggttttgc tatagcttct tacttagaga ttcctgtgac cggagtagct atttttggtg
     4741 cgattttagc agttgtggta gtcaatatta tgaatgtccg tcaactacca cttcaaacaa
     4801 ctgcagggga ggtagaagaa gatgaagaat tctgagatca aaaacagcaa aataacgaaa
     4861 aaagagctaa atcacgtatt ttggcgttct tttcaaatgg agttttcttg gaattatgaa
     4921 cgtcagatga atatggctta tgtttatgca atgattccga ttttgaaaaa gttatatcag
     4981 tcgaaagaag aaatggcagc agctttgaaa cggcatctag agttttttaa cacgacccca
     5041 catatcgtta cactgatttt aggaatcacg gctgcaatgg aggaagaaaa taaggatgat
     5101 cctgaatttg atgtgactgc gatcgatagt atcaaaacat ctttgatggg acctcttgca
     5161 gggttaggag attctttctt ttggggaact ttgcgattga ttgccacagg tgtgggtacc
     5221 tctttagcca tgcaaggaaa tattttaggc cctatcttgt tcattttgat tttcaatatt
     5281 ccgcacattc tgtttcgcta tctagcaaca ggttggggat atcgcttagg aacgggtttt
     5341 ttgaaaaaga tccaggcaaa cggtatgatg gaaagtttga cattaggcgc gtctattatt
     5401 ggactgatgg tcgttggagc aatgactgcc acgatgatca ccatcaatat tcctttaaaa
     5461 atcggttcag gagaaaatgc tgtgactgtt caaagtattt ttgatgatat cgttccaaat
     5521 atcttatgtc ttggagcatt tggcatagtg ttttatctat tgaagaaaga agtgaaacca
     5581 ttaacgatct tgatcggact agctttcttt ggaatttttg gttcgtggat agggattttt
     5641 taaaaaagga gcagagaaaa tgtttacgat gcaagattat atctatgaag aaaaggaagt
     5701 actaagtacg atcttgaaaa agaatgattt ttcgacaaga gaacatatga aaaaaacagt
     5761 gaatttactg atcttagcaa cgggttcatc ttataacgct tgtttagcag cgaagccagc
     5821 attggaatct tatggtgatc ttactgtcga tattcaagaa cctttttatt ttttcaatta
     5881 tggaaaactt tctccttcaa tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc
     5941 ttctactgtt gaagcagtaa aaatgataca aaaacaaggg ttacctgttg ttgctatcac
     6001 aaatgacgtg caaagtccac ttgctttaga agctgcacaa attattgatt taggtgccgg
     6061 ggtagaatca gtaggcttcg ttacaaaagg ctattctgcg actgttttac agttactact
     6121 cttagggata ggaattggaa taagtaaaaa taaaatcagc aaaaaaatag aacaagacta
     6181 catgcaacag ttgcgcaaaa taatcaacca tttaccagcg attattcaaa aaacggaaga
     6241 attttttgat ggatatcaaa gtttatttcg tttagcacag cgtttcgtca ccattggata
     6301 tggtccaaac tggggaacag ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt
     6361 tccgtcacaa ggatttgagt tagaagctta tatgcacggc ccatacctgg aggcagatgc
     6421 ttcgcatttg ctattcttta ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt
     6481 gcaaagatat atgagtcggt acgttggaga gactctcacc attaccacaa aaaaagcaag
     6541 aaacgagaaa acacttggat tagcaataga atgtgatgaa tatctgtctg tattagcgct
     6601 tgtcgttccg tttcaactat tcgcttataa aaccgcagta gctaaaggta ttgacttgaa
     6661 caaaaagatt tttgaagact ttgatacagt cttgaaaagt aaattataag aggtggaaat
     6721 catgctgaaa ttcaatgaag aagaacaaat caaagcaata aaaggcgcac tagctttacg
     6781 tccgcaagta gaagaaatca tcgataaact ttatgcggaa aaatttgacg ctgtctatta
     6841 tctaggaatc ggtggaacgt atgcttcctc gatgcaagct gtcacttata tgaatgggaa
     6901 aagcaacttg cctgtctttg tacagcatgc tgcagaatat tacactactg gaaacaaaag
     6961 attaacaaaa gattctatcg ttattttgtc ctctgttacc ggtacaactc aagaagtagt
     7021 gaaagcagta gaacaaatca agaaagtagg agcgacactg attggattta tcgataaagc
     7081 gaacagtaag ttgtctcagc tatgcgattt tgtggtcact tatccagctc ctggaacaga
     7141 acaaatcaaa ttttttatgg cagcagatcg attgatgtat ttgaatggag aattcgaagc
     7201 ttattcagaa tactatgaac agctcgaacg ttatttacca acagggttag tggaagcaga
     7261 gaaaaaagca gatgcatttg gcttatcatt tgcagaaaaa catcgacgtg atagtatgca
     7321 ttattttatt ggagcaggga atcaatgggg agctgtttat tcatatgcca tgtgttactg
     7381 ggaagaacaa agctggcttc catcgaaatc tatccatgct gccgagtttc ttcacgggac
     7441 gttagagatc gtagaagaaa caaccccagt cacattattt ttaggcgaag atgaacagag
     7501 agtgttatct gaacgtgtgg ctaaactttt gccaaaaatt tgcagcaatt atacattgat
     7561 agacacaaaa gactatccag tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc
     7621 tttcttattg atgcatgtag tgacacaacg catcgatgct catgtggagc agttgaactg
     7681 ccatccatta gagatacgcc gctactatcg tcagtttgat tattaggata gactggataa
     7741 aaagaatata aaaaataaag agagcagcga taactgctcc ctttattttt ttaaaatatt
     7801 ggcatcaatt acgttataaa acacattttt tgcattatct acagttcaac ttataatctg
     7861 tttaatggga catgtgggtg ccaaccattt tttgttatgc aatagtacta tttagttgtt
     7921 gcatggatcc gtagtaaaag tccaagtaca acgagttgcg ccagtttaca tagcgttttg
     7981 tatccagaat gttgactaaa acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt
     8041 ccgttgcagg acctgacaga tttagaaatg ttcaatctgt ctcttatgta ttttaaatca
     8101 atactcacta ggagaagttt cctaacctcc taatagtaat gctggtagta acattgtcaa
     8161 aactgcttcc tcttaggaac aagcaaagca taacgagtgt aaaaataaaa tgtagactat
     8221 tgtaacatat cattgattat ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa
     8281 aacaatggaa attagtccca actttaagaa atgtatacta taatggaaat tatcaattat
     8341 ttatttggag gaatatccat gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc
     8401 actgctttag cacaaacatt ggcagaaaac gggcatgatg tccgtatttg gggaaatgta
     8461 cctgaacaga ttgatgaaat caataagtat catacaaatc agcatttttt acctgatttg
     8521 acgatcccag aatcgattat cggatataaa gaattggcag aagcagtgga taacgcagat
     8581 gctattttat ttgttgttcc aacaaaagcg attcgttcag tcgcacagga acttattggt
     8641 aaaatgaata ctaagccaat cattatacat gcaagtaaag gactagaaca agatacgcat
     8701 aaacgaatct cagaagtttt ggctgaagaa attccagaag aaaaaagaca ggcaattgtt
     8761 gtattatctg gtcctagcca tgctgaggaa gtggcagtac atgatatcac aacaattaca
     8821 gctgcaagta ttgatcaaaa agcagcagcc tatgtacaaa aactgttcat gaatgattat
     8881 ttcaggattt atacaaataa tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat
     8941 atcattgcaa ttggtgccgg cgctatccat ggattaggat tcggagatga tgcaaaagca
     9001 gcaatcatga ctcgaggatt ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat
     9061 cctctgacat ttatcggttt gagtggtgtg ggggacttga tcgttacttg tacaagtgtt
     9121 cattctcgca attggcgtgc cggtaacttg ttaggacagg gacataagct ggaagaagtt
     9181 ctagaaaaca tgggaatggt cgttgagggt gtggcaacga caaaagcagc agtagagcta
     9241 gctcaacagc taggtgttga gatgccaatt actcaaacaa tctataatgt tttgtataat
     9301 ggagaagata tcaaaaaagc agcaaaagaa atcatgttac gtgacggaaa aatggagaat
     9361 gaattttctc ttcgttaaat agatgatcaa tagttaatcg t
//
LOCUS       DACDKL010000097         9363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDKL010000097 DACDKL010000000
VERSION     DACDKL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.097"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..995
                     /locus_tag="IV329_001811"
     CDS             21..995
                     /locus_tag="IV329_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8940983.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1017..1709
                     /locus_tag="IV329_001812"
     CDS             1017..1709
                     /locus_tag="IV329_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8940984.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            1675..3183
                     /locus_tag="IV329_001813"
     CDS             1675..3183
                     /locus_tag="IV329_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8940985.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            3257..4360
                     /locus_tag="IV329_001814"
     CDS             3257..4360
                     /locus_tag="IV329_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8940986.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            4363..5700
                     /locus_tag="IV329_001815"
     CDS             4363..5700
                     /locus_tag="IV329_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8940987.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            5687..6565
                     /locus_tag="IV329_001816"
     CDS             5687..6565
                     /locus_tag="IV329_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8940988.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            6562..7485
                     /locus_tag="IV329_001817"
     CDS             6562..7485
                     /locus_tag="IV329_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8940989.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            7482..7763
                     /locus_tag="IV329_001818"
     CDS             7482..7763
                     /locus_tag="IV329_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8940990.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            7767..9335
                     /locus_tag="IV329_001819"
     CDS             7767..9335
                     /locus_tag="IV329_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8940991.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         3048 a         1552 c         2062 g         2701 t
ORIGIN      
        1 acaaaactaa ggaggggctt atggaaagtg taaagaaaca agaaccattt caactgacgc
       61 tatcgaaaaa agaagaaaag ctcagaagac gaaaaaaaat aattgcagga atcaaaacga
      121 ataaattttt atatgtgatg attctgccgg gagtccttta tttcttgatt tttaggtatc
      181 ttcctatggg gggactcgtt attgcttttc aagattatca accattttta ggaataatgg
      241 gaagtccttg ggtcggtttc aaacatttca ttcgtttgtt tacagaaccg acattcttta
      301 cattattaag aaatacgttg atcttgttcg gattaaatat cttcgtgttt tttccgatgc
      361 caatcatttt gtctttacta ttaaatgaag taacgaacaa acgattgaaa aacgggattc
      421 aaacgatcat ttacatccct cacttcatgt cttgggtaat cattgtttcg attacttatg
      481 tatttttgaa tgttgacgga ggggtattga atgaattcct tgcaacaatg ggatttgaaa
      541 aaatcagttt tctaacatcc cctgaatgga cacgcacagt atatatcgga caagtgatct
      601 ggaaagagtt gggctggtct acgatcatct acctttcagc gataactgca gtcgatacac
      661 agttgtatga agctgcagaa atggatggag caggacgctt acgcaaaaca tggcatgtta
      721 cattgccggc aattcgtcca gtaatcatta ccttgctgat tctaaaaatc ggtcagacat
      781 tagacttagg gttcgagcat atgtacttgt tattgaattc attgaatcga agcgttgcag
      841 aaattttcga cacctatatt tatacagctg gattgaagaa tggacagtta agtttcagta
      901 cagcaatggg attgtttaaa ggggtcgtag gattgtttat ggtaatttta gctaataaga
      961 ttgccaaaaa gctaggagaa gacggcgttt actaagatag aaagagaggg aaagatatgc
     1021 tgcagtcatt agataaaata aacggaaggc taaatcgtat tttacagttg gtttatttga
     1081 attttctgtg gggcggttta gtcatcgcag gtttgggagt ttttactttc ggtcctgcat
     1141 cgtatgcgat ggttgcagtg attagaaaat ggctgcgctc aaaagaaacg tttcctttga
     1201 cgaaaacgta cttcacatat tttaaagaaa actatcgtga aaccttgctg atgagctgga
     1261 tatatggcgc catcgggatt gttttacttg tggatctttc tttcatacac aattggtatc
     1321 ttaggatcgc tttgtatatt gctgcgttca tatatgtgct ctcagcactc tatattttcc
     1381 caatcatggc tcattatgat tggaaaggat attttttcaa aatgaaaatg gcatttttat
     1441 tcggattctc acatcttcat tactcattag tgttgatatt aagtatggta gggatttatc
     1501 tgatgatcat ccaaacaatg ccaggtgttt taacctttat cggtggcagt ctcttctttt
     1561 ccgcagtcac gtggacagca aattctatat tttgccattt ggaatggaag aactcatata
     1621 cagaaaaaac agaagaaaac aaaaatatta ttaaaacagt gaaggagaaa atttatgaaa
     1681 aaagcaacgc tcgtaaaatt aggtcttagt tttgcaggaa ttctagcact tgccgcatgc
     1741 ggcagcagtt caagtaacag ttcttcagag acagaagatg gaaaagctaa aatcacttgg
     1801 ttaaatattt tgcatcatgc ttcaccgcct acagacacga ttgtgaactt gatcgaagaa
     1861 aaaaccgata cggaactgac attcaactgg attcctgatg cttcaaaaga agaaagatta
     1921 acaactgcat tggcttctga tgaactagga gatatcgtta cacttcaaat tgcgatgttg
     1981 aaaaactctt ctgtccgcaa tgcgttaaaa tcaggcgtat tctgggatgt atcagaatat
     2041 ttaaaagact ataaaaattt aagtcaaatt tctgaagata gaatcgaagc tgcgagtatc
     2101 gacggtgcct tgtacggcgt accgatacaa aaagactacg ctcgtgctgg cttagtcatt
     2161 cgaaaagact ggttggataa tctaggacta gatgttccaa ctaccatgga tgagctttat
     2221 gaagtagcta aacaatttac agaaaacgat cctgatggaa atggtgtgga cgacacagtt
     2281 ggttttggcg atcgtgcgcc tagtgatatt cgttatacca gctttaaact tttcacttct
     2341 tattttggag caccaaatgg ctgggctgta gacgataatg gcaaattcac tccagaattt
     2401 gatacagaag agtatatgaa agctttgaat ttttcaagag atctatttga gaatggctat
     2461 ttggcacaag actttgcagt tacccaaaag actgatcagc aagaacaatt tgcacaagga
     2521 agaactggaa tctacactgg aatggtggat atcaagaatc tgaagacgat gggtcaaggt
     2581 attcaagatg atatggaatt agtgcctgta aacaagatct cagatggtga aggcggcggt
     2641 tatcatgttt ggtcagaagg aaatggtatt ggcggcttgc tagcatttcc caaatctcaa
     2701 gtaaaagacg aagcacaatt gaagaaatta cttcaatttg ttgatgatct tttagaagaa
     2761 gatgtgtata aattgatgac tggcggtatc gaaggaacgc attatgaatt gaatgatgat
     2821 ggttctatcg aatttatcga tatggatctt tggcaacaag aggtgcaacc gttatcaagc
     2881 tctcgtccta gtgaagtgac gttctctttc aaagatacag accctgaaaa agaattctct
     2941 aatcaattga tcaaagaaaa tgaagaattt gctgtaatgg atccttctgt gccattggat
     3001 tcaccgacga acaatgaagt aggatcagaa attgaaaaaa tcgtcattga tgctacggtg
     3061 caatacatca tgggacagct tgacgaagat ggattcaaaa aagctgtaga agactggaaa
     3121 gcacaaggag gcgatcgaat cacacaagag tacgaggaag catacaaagc agcggcacag
     3181 taatatagac gattgggaca aatacttctt ggtatttgtc ccaaatccta ttttaatttt
     3241 taaaggagag caaataatgg aaaactggtc aaagaaatta gcaaaaagta tcatggaaag
     3301 aacacctcgc ttatatgaag aaaagtggta taaaggaaaa tggtcttatg actatggcgt
     3361 cgtacttaaa gggttccaat tactttggga acgaacccaa gaaaaaattt attttgattt
     3421 cataaaagac aatatcgact atttcgtgca agaagatgga acgatccgtg gctacagcgt
     3481 tgaagaatac aatatcgacc atgtaaatac aggaaagctt ttctttttat tgtacaaaga
     3541 aacaggcgaa gaaaaatata aaaaagcagc agaactcctt tcccagcagt tggcaaacca
     3601 tccacgcaca tccgaaggag ctttttggca taaagaaatt tatccttatc aaatttggtt
     3661 ggatggctta tatatgggtt ctccttttta tgctgaattt atccagaatt ttagtgaaga
     3721 aaaagattac caagatgttt tgagacagtt tgaaatcagt tataaacatc tattcgatcc
     3781 aaaaacaaat ttgctgattc atgcatggga tgaaaagaag gtccaaccat gggctgatcc
     3841 aaaaacaggt ctatcccatc atttttggag ccgatcgatt ggctggtatt tgatggctgc
     3901 agcagatacc tatgaattac ttcatgaaga aacagattgt tctttgctga aagaaattct
     3961 agaaaagaca ctggctgcat tacttccata tcaagaagaa aacagcggga catggtatca
     4021 agtaacaaca gaaacaaaac ggaaaggaaa ttatctggaa gcttccggca gtagtatgtt
     4081 tgtttacgca atggctaaag gaatacgttt gggactgctg aacaaagaag aatggcttcc
     4141 acaagtgaag aaatcacatc aaggattact tgatgaattt gttctggaaa caaaagaagg
     4201 ctggctgaac ttgaataaaa actgtcaagt agcagggtta ggcggagcag atcagcgaga
     4261 tggttcatat gcttactaca tcagtgaacc aattatttgt aatgatcaaa aaggcgttgg
     4321 tgcattcttg caagcattga ttgaagtgga ggcattataa atatgtatga tattttgaca
     4381 tttggcgcat caattgatga attgaatact gaagcaatac aacaagccat tgatgctgca
     4441 gcaagtgacg gaggcgggac cgtagttgtt ccagcaggtg aatttctaac aggagcttta
     4501 tttttaaaaa gcaatgtcga acttcattta agcgcaggag cggttctgaa attctctgac
     4561 gatccaaaag attatcctgt tgttcattca cgttgggaag gagtccatcg aaaagtttat
     4621 gcttcctgta tctatgctca aaatgtagag aacatctctg tcactggttt cggtacatta
     4681 gatggaaatg gaaaaaaatg gtggcatact tttagaaatg aaccagataa tctcgcttat
     4741 cctcgtccta aattgatgag tttccataac tgtcatcgaa ttactgtgaa agatattaaa
     4801 ttgatccagt caccaagttg gacgatcaac ccgattcttt gcagtaatgc aacgtttgat
     4861 aacttgacga tcttgaaccc agcggattca ccaaatacag acggaattga tccagaatca
     4921 tgcaaaaatg tgcgtattag taattgccat attgacgtgg gagatgactg tattgcaatc
     4981 aaggcaggga ctgaagatac gtacgaaagg atcgcttgcg aaaatatcac gatcacaaat
     5041 tgcacgatgg tccatggaca tggcggtgtc gtattaggaa gtgaaatgag tggtagtatt
     5101 cgtaatatta ccatttcaaa ttgtatcttc caagaaactg atcgaggaat acgattgaaa
     5161 tctagacgag gtcgaggcgg gatcgttgaa gatattcgag tatccaatat agtcatggat
     5221 aatgtgatgt gtccatttat actgaatctt tattatttct gtggtccgcg agggaaagaa
     5281 ccgtatgtat gggaaaaaaa ggcttatccg atagatgaac ggacatcagc attcagaagg
     5341 atccattttt cgaatatcac ggcaaggaat gtccatgctt ctgcaggttt tatttatgga
     5401 ttagcggaac aattcataca agaaatcaca tttgacaata tcgatgtctc gatggcccaa
     5461 gatgcagttc ccggtgttcc tgctatgatg acaggaatag aggaaatgag cagacaagga
     5521 ttttatattg gctttgcggg taaggtttcc tttaaccaga tgtcgattga aaatcacgag
     5581 ggaccagcat ttctattaga aaatactaaa gaaatcagta ttgagaaatt ccaatcaaat
     5641 caatcagaaa atatggatga actggttgtt cgtaaaaact ttgcttatgc agttgaatga
     5701 ttttttaggt gctccatcta gcagacatac ccctagtgga aaacgattat accgagaaaa
     5761 acaaaacggt tttatgcttg aatactatca attgtactta aatgaattgg aaacagtacc
     5821 agccctctat gcttatccag aaaaagaagg accgtttccg acggtccttt acctgcattc
     5881 tcacggcgga gatttttcag tcgggaaaag tgaattgatc catggtgctc cttacttagc
     5941 ttctccatct tttgcagaag tactgacagg aatgggatat gctgtttggt cgattgatgc
     6001 ttggggattt gaagaacgcg gaggtatttc tgaaagcgag ttgttcaaac aatttttatt
     6061 aacaggaaaa acgttatggg gaatgcgtat ttatgatgtg atcagcttga tcgattattt
     6121 ggagacaagg gaagacacag atactcaacg gctcgcaacg attggtcttt ctatgggagg
     6181 cttattaagc tggtgggtga cagcattgga ttctcgtgtc aaagtttgta ttgatctagc
     6241 ggcacaggtg gacatcgaaa cacttttgct acatcgccgc ctagatcatc atgggtttta
     6301 ttactatgtg ccaaatctat tgacagctta tacaacgtta gcaatccaag aaaaaatcgc
     6361 tccgcgccct cgtcttagct tagttggaaa aaacgatacg atgtgcttgg acgaaggcgt
     6421 actgaagctg agaaaagggc tggacaaaaa gtatcaatca atgggtagtc cagaaaactt
     6481 ttcttctttt tctttaacag gcggtcatat ggaaacacaa gaaatgagaa atatttggaa
     6541 acaattcata agggagcatt tatgaggatg aaagacaaac tgataattgg aaagaatgct
     6601 atagataccc cgacggattt tgaaactatc caagaaggaa tcgattattt ggcaggcctg
     6661 ccggatagcc atccaaaggt cttgatagta aaagaaggaa gatatgatga atatatagaa
     6721 agcagattat ccaattttaa acttgtggga gcaggaacag tagttatctc cggtaatcgt
     6781 tttgcaaaac aggagtatct tgacggtagc cagttaggga ctttccgaag tgctactttt
     6841 ttcttagaag gatcgaatgt cgtattagaa aatcttcaca tcgaaaatac agcaggtcca
     6901 ggagaaaagg taggacaggc tgttgcttta ttcaattcag gagatcgcgt atctttaaaa
     6961 aactgtcgac tatctggtta tcaggatacc ttatgcacgg gtccgttgcc tgatttgcaa
     7021 aaagatggta ctcctttttg tacaccggct tctgccaatc ctgagtattg tcgtcagacc
     7081 tatgaatatt gctggattga aggaacggtg gatttcatat ttggtggggc agatgccgtt
     7141 tttcatcagt gtcatatcca tagcttagcc agtaaaaatc ctggatacat cacagcagca
     7201 tctacgccga aaaatcaaaa aacagggtat cgttttgaaa gctgtacctt gactgcagaa
     7261 cctgatacta gaaacgtcta tttaggaaga ccgtggcgtc cgtatgcaaa tgtcttattt
     7321 gaagcgtgta agataggtgg gcatgttcac ccaactggtt gggataattg ggatgacaag
     7381 aaaaatgaac agactgtcaa attctctgaa cgtaataaca attatgaaac atcgatcaaa
     7441 agacctgaat ggatacattt agaaaagcag ggggttcaga aatgaaaaga aaatgggaac
     7501 tattgctagg aatggtcggt ggcagtctat ctttgatttt ctttggcggg ttagcagtta
     7561 ctttaagtaa tatgtctgcg tcagagttta aaaaaagtta tcagtcgctt gctgtagatc
     7621 attcaacttt gtctttagaa aatacatttg gattattaca ggacatgact ggtctgtttg
     7681 ccgttgtgtt gtttatttca cttgcttttt tagcagctgg ggcattcagt ttatatcgag
     7741 taagaatgag aaaaggagcc tgaatcatgc agactatcaa agtgaaaaat cctattttaa
     7801 gaggattcaa tccagatcct aatatattaa gagtgaaaga gcggtattac attattgtct
     7861 cctcttttga atggcttcca ggaatccgag tatatgagtc gaaaaattta gtcaactggt
     7921 cacatacaac ggatattttg accactcaag tatctttgca aggaaatcct acaaatggaa
     7981 gtatatgggc gccacagatc agttatgctg atggattgtt ttatcttgtt tacacagatg
     8041 tcaaaagtgc aggccgtccg tttaaagatg tccacaatta tttgatcact gcatctgaga
     8101 tcacaggtcc ttggtccgaa ccaatttatt taaatagtaa cggctttgat ccttctttgt
     8161 tccatgatct tgaaacggga aaaaaatggc ttgtcaatga actatgggat tatcgtctgg
     8221 acacaccgaa taaatccagc ggtatcgtat tacaagaata tgatgcagag aagaagaagc
     8281 ttgatggtcc catctataaa atatttgcag gtacagaatt agcgaaaacg gaggcaccac
     8341 atttgtattt tcgtaacggg tattattatt tgatgacggc agaaggcggt acaggaagag
     8401 gacattcagt tacagtttgc cgagcaaaga atattactgg tccttacgag cttgatcctg
     8461 gttttcctat gctgacagct agtgacaaac ctgcttctac attgcaatgt acaggacatg
     8521 ggagcttagt acaaaccccg tcaggaagat ggtacatgac atatctttgt accagaccat
     8581 taaacggtgc ggctattttg ggaagagaaa ccgctattca agaagtgtat tggacgtccg
     8641 acgattggtt aagacttaaa gatagccaga caactccgat ggaagaaata atgattgaga
     8701 caaatgaaga ggtacaacaa gaaaaagatc atcagtttat ggatacattt gaaggagagc
     8761 taaagaagaa ctggaatgcc agacgcattt tgccaaatgc tgattggtgt gatacaaaat
     8821 gtagaccagg ttttttacgt ctgatttccg gtgaatctct tcaatctacc ttcgaacatc
     8881 atttgttagc tattcggcaa acggatttta cttttgatgc ggagacagca tttgaataca
     8941 tgcctaataa ttttaatcag atggctggac tggtactgta cttaaatgaa tccaattata
     9001 tttattgtta tgttacttgg aatgaggaca agggaaaatg cctacgtatg atagaatcag
     9061 aaaacggagt agcacaaata gaagattgga cgattccact cacagaaaag aagacttttt
     9121 tgaaagtcac tgtaaagaaa gagacagcac agtttcattt gaaagacgta tcaactgaag
     9181 aatggaagaa agtaggtccg caaaaaaaca tgtgtatact ctcaggaggg tttactggaa
     9241 attttatcgg gatagcagtg catgatctaa ataaaaaaag aggaagttat gctgattttg
     9301 agtttttcaa gtatgatgga aaagatcatc tgtaatttaa ttctttaata aatgaggttc
     9361 act
//
LOCUS       DACDKL010000098         9356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDKL010000098 DACDKL010000000
VERSION     DACDKL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.098"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            114..314
                     /locus_tag="IV329_001820"
     CDS             114..314
                     /locus_tag="IV329_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8940992.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            461..3250
                     /locus_tag="IV329_001821"
     CDS             461..3250
                     /locus_tag="IV329_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8940993.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3297..3824
                     /locus_tag="IV329_001822"
     CDS             3297..3824
                     /locus_tag="IV329_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8940994.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3845..5035
                     /locus_tag="IV329_001823"
     CDS             3845..5035
                     /locus_tag="IV329_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8940995.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5075..6373
                     /gene="asnS"
                     /locus_tag="IV329_001824"
     CDS             5075..6373
                     /gene="asnS"
                     /locus_tag="IV329_001824"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8940996.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7549..8223
                     /locus_tag="IV329_001825"
     CDS             7549..8223
                     /locus_tag="IV329_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8940997.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8220..8561
                     /locus_tag="IV329_001826"
     CDS             8220..8561
                     /locus_tag="IV329_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8940998.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8591..8950
                     /locus_tag="IV329_001827"
     CDS             8591..8950
                     /locus_tag="IV329_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8940999.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9154..9333
                     /locus_tag="IV329_001828"
                     /pseudo
     CDS             9154..9333
                     /locus_tag="IV329_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         3127 a         1679 c         2027 g         2523 t
ORIGIN      
        1 gagtagtttt tgttcggtaa tctgtggtag cttgtttcaa ataaacatct tccagatata
       61 caccattcaa gtaattgcaa aatatgtgtt gatacacaag gaggattttt attatggaaa
      121 caggtacagt aaaatggttt aactcagaaa aaggatttgg attcatcact gcagaaaacg
      181 gaaacgatgt attcgttcat ttctcagcta tccaaggcga cggattcaaa actttagaag
      241 aaggccaagc agtaactttc gatgttgaag aaggccaacg cggacctcaa gcaacaaacg
      301 ttactaaagc ttaattaacc tatactaaaa aataaacgcc cagacatttg cgtgtctggg
      361 cgtttttgtc tattttcttt aaaaagcgat gagagtattt ttctgcactt tgtagtataa
      421 tttcaaaaga acattagagg caggagaaga ggaagatatt ttgaaaccaa gaacttatgc
      481 agtcgttgat atcgaaacga ctgggacgaa tccaaaagaa gatcggatca tccaatttgg
      541 ctgcgtcatg attgaatcag gtcggatcac ttcacgtttt tcgatcgata ttcatccagg
      601 gcggaagatt tcaaaacaaa tccagcattt aacagggatc acgaatcaac gcgtccaaaa
      661 agcgccttat ttcgaagatg tcgcccaaac gatttacaat cttttggcag acaccatatt
      721 tgtcgcgcat aatatttatt ttgattataa ttttttgaac catgaactca tgcgatgcgg
      781 acttcctagt ttgaagatac caggcatcga tacagtagaa cttgcacaaa ttttcttgcc
      841 gacggaacca agctttcgct tagctgattt gtcagaaagc cttggattta ttcatgagaa
      901 tcctcatcag gcagatagtg atgccgaagt gacaggtcaa cttcttttgc tgatcgaaga
      961 acggatgaga aaattgccaa tcatcacgtt agagcagatt gcgcgattaa gtagacacac
     1021 aggtatggat acgagtcgtt tcatttatca tgtgattgaa gaaatgaaag aaaaaccaga
     1081 gccacttgat ccctcattag aaatcgtcga tgggctagct ttgcagaaaa aagaagtgga
     1141 actattcaca tccgtccatt atggcgagcg gacatatcca agaaaaaaac aagcaaaaga
     1201 aaaaatgttt ggtaaaacac tgatgtatcg aaaagaacag aacagactga tgaatgcggt
     1261 ctacgaccat ttcacaaaag atgaatcaaa agatttgatg atcgaagcag ctacaggaat
     1321 gggtaaaaca atcggttatc tgctgccatt gagttatttg gctacacctg aaaaacccgc
     1381 tgtcatcagt accgtttcgc tagttttgca gcagcagatc atagaaaaag acattccatt
     1441 gctgaatcaa ttgctagatc agcccataca acctgtaatt atcaaaagct atcgccacta
     1501 tattgatttg cagcgtttta aaggcacact tgtggaaccg ccagaacaaa aacaatatgc
     1561 tttgtatcaa atggcggtct tagtttggct gacgcaaacg aagactggtg atttggatga
     1621 attacattta acaaatttga accattcatt ctttgcagat atcgcgcaca gaggtattgg
     1681 atttttagca agaaatcagt cattttacga acaagacttt gttcgtttct tgcaaaagaa
     1741 aatcaggcaa agtaattttt tgattgtcaa tcatgcgttt ttagtacaag aaacacagcg
     1801 aaaagaacca ttgcttccag ctagtccatt tttgttgatc gatgaagtac atcatttgcc
     1861 ggaaatcgct gaaaaagtaa atgacaaacg attaagtatt aactatttcc ataaacaaat
     1921 ccagcaatta gaagaaccaa atcagctgtt tgaccgaata caaagctatt taccgaaaga
     1981 acatgaagcc ttacgctggt tatcgattta tcgagaagaa ctttctgcgc tatgcgaagc
     2041 acagatttgt atggcagaag gattaatgag tttagtgagc ggcaagcaac ctaacggaca
     2101 gtatccagaa gaagtcatga tgacaaaaga gatgttggat cagctgccac tggaatctgg
     2161 gacagccatt gatcacctgc agctttttta tcaggagatc cagcagctac agtcacaaat
     2221 gcgtctgact tttgagatta tccaagaaag ctggacaaac cagatgcgga tcgatttagc
     2281 aagtttatat agcctatttg atcaattaga caagcaatac ttgctgatgg atcaatggct
     2341 ggaagactgg cgagatcatt tgatccattg gttcgttcct gggaacacag cgcagcagat
     2401 gatttttcat ctccatgatt ttcaagcggc tatgttgccg tcgactgttt ggtatcctcg
     2461 atatgatcgt attctttata ttggaggaac gctgaagatc ggccctaatc gacactatct
     2521 ctctaagagg cttggcattg aagcagcacc tcttaaagtg attgcctctc cttatgatta
     2581 tgagaaacag gcaaggatct atgtgccaaa agaggcacct gccgtccatc aagtcagcaa
     2641 tcaagcatat atcgattatt tagagacagc acttacaggt ctgatcgaac aagaagatcg
     2701 gccaatactt gtgttattca cttctcatga tgttttgcag aaggtctatc agaaaatgca
     2761 tctgtctatg ctagaaaaag gacgagaaat actagctcaa gggtttggtg gaagccggga
     2821 gaagcttttg aaacgcttca ttttgtcaga aaaaagcgta ttgtttggcg ctgacagctt
     2881 ttgggaaggt gtagatttgc ctggagatgc tttgcagatc gttgtagtca cacgtttacc
     2941 cttcgataat ccgcttcgcc cgatggtcaa agctaggaac gcttatttag aatcagaggg
     3001 gatcaatcct ttttaccagg aatccgtacc taaagcagct ttgaagttac gccaagcatt
     3061 aggtcgcttg ataaggacgg agacagatcg tggcgtattg cttattttgg atcgaagact
     3121 agtcactgca aaatatggcg cacggatcat ccatgcatta ccgaaaaaag taccgatcaa
     3181 agagctagct atggaggaaa tctatcagga tatccatgaa ttcttaaaga aaaatgaaga
     3241 aggaagttag aggagctgtc ttttttcata attaaaatct gctataatga aaaggaatga
     3301 agataaagga gcgacgaccc ttgaagaaaa aacagacaaa ggaaaaaacg attaatcgcg
     3361 tgttacttgg cttgatcgta tttttattag cggtcatcgt gatcagctcg attttttact
     3421 tgcgttccag tcgtccggcg gtgcaggcga agaaagaagc agtagaaatc gcaaagaaat
     3481 atgcaaatct tgaggaaacc gatcattttt attggtttac tcgaaagaag acatactttt
     3541 cactgacagg tacaaatgat aaaggaaaag aaatcgccgt tatcattcct aaatctggtg
     3601 aaaaagtaaa agtattggat caaacaaagg gactgactga aaacgaagca aaacagacaa
     3661 ttgctgcggc ccatcctgaa atccaaatcg aaaaagctgc tttagggatg tatgatgatc
     3721 agcctgtgtg ggaagtagta ggaaaagaaa caaatgggaa actgaattat tacctgattg
     3781 gctttgaatc tggagatgaa gtcaagacaa tcaagggaat ttaaaaggta aaggaatgaa
     3841 tgggatggag ttatcaaaaa gaacaaaacg attagaaccg tctgtcactt tggcagctgc
     3901 ggcaaaggcc aaggcattaa aagcaaaagg cagagatgtg ctgagtttga ctgtgggaga
     3961 accggatttt gcgacaccag aaaacatcca agaagcagca atcgaagcaa tccgcaatgg
     4021 gaaagctagc tattatacac caacagcagg aatcccagag cttcgacagg caatcgttga
     4081 ttatctgaag aaaaactatg gacttgtcta tgaaccttca caaacagtcg taacagacgg
     4141 agcgaaattt gctttgtata cccttttcca aacgatcctt gacccgcaag atgaagtcat
     4201 cattcctgtg ccttactggg tcagttacgg tgagcaagta aaactggcag aaggacttcc
     4261 tgtctttgtg aaaggggaag aatccaatag ctggaaagtt actgttgaac agctagaaca
     4321 agcacgttca gaaaaaacaa aagccgtaat catcaattct ccatcgaatc cgactggaat
     4381 gatttatagc aagaatgaac tgcaggcaat cggtgaatgg gcagtaaaaa atgatatttt
     4441 gatagttgct gatgatattt atggccgttt agtttataat ggcaatgagt ttactccaat
     4501 tgcaacgatt tcagaagcaa tcaaaaatca gaccatcatc atcaacggcg tgtctaaaac
     4561 atatgcgatg acaggctggc gcattggtta tgcggtggga aatcctgtga tcatcaatgg
     4621 aatgatcgct gttgcttccc aatcaactag caatccgaca gcagtcagtc aatatgcagc
     4681 tgttgaagca ttgaaaggtg agcaagatac agtagaagaa atgcggaacg cctttgaaga
     4741 acgcctgaat acgttatatc cgcttgtggc agaactgccg ggtgtttctc ttgaaaagcc
     4801 gcaaggagct ttctatcttt ttcctaatgt aaaagaaacg ctacgcatgt gtaagtatga
     4861 aaatgtcaca caatgggtag aagatctttt agaagaaaca ggggtagcgc ttgtgactgg
     4921 cgaaggcttt ggggcaccag aaaatgtccg catgagttat gccactgatc gcgctacgtt
     4981 agaagaagcg gtacgcagaa tcaagcaatt tattgagagt aaaagtcaaa attaatcctg
     5041 aagaaattca aggaatataa aaggagagtc atacgtggaa caaattcaaa tcatcgattc
     5101 gaaaaaccat gtgggagaaa cagtaacaat cggcgcttgg gtcgcaaata agcgatcaag
     5161 cggaaaaatc gcatttttac aattaagaga cggaacagcc tttttccaag gtgtagttgt
     5221 aaaaagcgaa gtgtcggaag aagttttcca aacagctaaa aatctacacc aagaaacatc
     5281 cgtgttagta actggagaaa tccgtgaaga cagtcgttca aaatttggtt atgaaatcgg
     5341 aattacaaat gtggaagtgg taagcgagag ccacgattat ccgatcacac caaaagaaca
     5401 cggaacagac ttcttgatgg atcatcgtca tttatggctg cgttcttcaa gacaacatgc
     5461 gattatgcag atcagaaatg aaatcattcg tgcaacgtat gaattcttta atcaacatca
     5521 ttttgtcaaa attgatccgc cgatcttgac tggttctgca ccagaaggca ctacggatct
     5581 attcgaaaca aattactttg atcaaaaagc ttatttatct caaagtggcc aattatacat
     5641 ggaagctgct gctatggctt tcggaaaagt cttttcattc ggtccgactt tccgtgctga
     5701 aaaatcaaaa acacgacgtc atttgatcga attctggatg atcgaaccag aaatggcttt
     5761 cactcatcaa gaagaaagtt tagaagtaca ggaacaatat gtagcattct tagtacaaaa
     5821 tgttttagat cattgcgaat atgcattaga tgtactagga cgagacaaag aagtcttaaa
     5881 aacttataca aaattgccat tccctcgtat tacttacgat gatgcaatcg aattactgaa
     5941 gaaaaatgga tttgatgaca tcgaatgggg cgaagacttt ggttcacctc atgaaacgtt
     6001 cattgcgaat tcattcgaac agcctgtctt tatcctaaac tatccaaaag ccatcaagcc
     6061 attctacatg aaaccacatc caacaagaga agacgtggta atttgtgcag atatgatcgc
     6121 accagaaggt tacggtgaaa ttattggcgg aagcgaacgg gcaacagact atgatttctt
     6181 gctagaacaa atccgtaatc acggtttgga tgaaaaagaa tattcatggt atcttgatct
     6241 acgtaaatac ggttctgttc cgcactctgg ttttggtcta ggccttgaac gaacagtcac
     6301 ttggattgct ggtatcgacc atgtacgtga agctattcca ttcccacgtt tgttgaaccg
     6361 aatttatcca taagaggtga tgaaaaagct gttctgcatc aagtaattag aacagccaag
     6421 caaaaacagc tgttcatgtt tttgaattgg cgggacttat taccgcagat gtaagctttt
     6481 gaataccgtt tagtggagag gttgtgaaaa agctgtttag tttcgagtat taagaaaaat
     6541 tttgtggaaa tggctttcca catttttgca taaatttggc ttaataccga gaaactggct
     6601 tttgaacacc gtttaaggaa gaggttgtga aatcagtgcc ttgacctgag tattagagaa
     6661 acaaaacgga aaaatagctt ctcatatttt accgtttttt tagctaatat cgagggtcag
     6721 ctgattgaac accgtttata tcaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa
     6781 ggagtaattt caaaaaacag tttttcatgt ttgatgacta ttgtgacttg tgaccgagga
     6841 gttggccgga gagcaacgtt tatttaaaaa aaagaactgc gaactctcgc agttcttttt
     6901 ttaaaatacg ggataaagga agtttccagc agacaagaga agaaagcaaa agaaattgct
     6961 atttgattca caaggttcta taacgaaatc gcaatcaatc aaacatatac aaagaaggtt
     7021 attcaaatgg aaatctgatt tgatgatgct tctgctgttg aaataaagca gcatctagta
     7081 cctgaaaaca aactaatagt aacattcgaa gatggagtag gtccgtattt tcagcatgca
     7141 atgatccata tgtaagttta attcatcctt gaaatcatcg gaccagataa agttatgacc
     7201 aatctatccc aagcaatcta gaagagatat tagtaaaaga acatttactg gaagtcttag
     7261 atgagcctat aaatatatgt tctgatggcg tcatcagtgg tctgattgac ggaagcttag
     7321 gtgtgatcga tttctctgga ccaaacaacg aaggaatcaa ggggaatccg gcacgttaag
     7381 aaaaaaagta taaaaaacta gacacttgat gagcgttcat tcaattgatt gaagggacta
     7441 ggctggtgtc attttttata catatatttt ctctttcgtc ttgatagggc ttatgggata
     7501 atataaggaa gaaaatcatc gttttattca aaaaggagtt tttgacaaat gaaaaaacgt
     7561 atattattta tcggtgacag cattacagac gcaggaagag accgagcaga agcaactgat
     7621 ctaggtaaag gttatccact tttgattgcc ggaaaactaa tctcccaata tccaacgatg
     7681 aaatttgaat gcatgaaccg tgggatagga ggagacaaaa ttgcagatct gcaagtacgc
     7741 tggcaagaag attgtttagc tttacaacct gactatgtat cgattttgat cggaatcaat
     7801 gatacctggc ataatactag cgatcaggct atattcggaa ctgagaaatc tgcgcagcaa
     7861 tttgaatccg tttatcgaaa attactaaat gaacttcaaa cagcggaaat ctgcaacatc
     7921 atcttgatgg agccgtttgt acttcctata accgacgacc gtcatggctg gcgtaaagac
     7981 ttagacccta aaatccaaat tgttcgtaaa cttgctaagg aatatggcgc attgctggtt
     8041 cctttagatg gcatattcaa tgctttaggg attgcttatg gtttttcgaa atatacaaga
     8101 gaagatgggg tccacccaac gattggcgga catgagatca ttgcacaaag ctggatagat
     8161 gcaattgccg cagtcgattt ttttagtgga gataaaaatg agcgaaagaa aagtgagaaa
     8221 tgatggaaat acggtgggca gtaaaagaag atttacctca aatcaatcag ctgattactg
     8281 atcatccaga atcattcgta aaagcaaaaa cggcagctga cctaaatgat aagaataaac
     8341 tattgctcgt tgctgtaaca aaagaaaaac ggattatcgg atttgcgcaa gcggacgtgc
     8401 aacagtcaga agaacgggtg gatcagcgac catcaggaaa actaatccaa cacacattag
     8461 taacaaaaga aaatacggaa gaaatccaaa aaaaatttgc agaatacttg atgctggaag
     8521 ctaaaaaaag aagaataaaa tcaatttttg cccagtttta atcaaaaaca gtaatagaag
     8581 gagaaataca atgagttgtt taggaaatgt catctggttt atattcggcg gactaatcgg
     8641 tggtcttagc tggttagcag cggggatact ttggtgtatc acgatcatag gtatcccaat
     8701 cggtatgcaa tgttttaaga tcgcctccct ttcttttgcg ccttttggca aagaagtaat
     8761 ttatcaccag tctggcatgt cattgatcgt taatatcatc tggctgatcg tttcaggcct
     8821 accaattgct ctcgcacatg tcgtcagcgg catccttcta tgtatcacga tcatagggat
     8881 accttttgcc aagcaatctt tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga
     8941 aattgtataa aaagagaata ataaaggtta gaaaacgcat agagtcaggt gggatacatg
     9001 tatttcacct gactctatgt gttttcctgt ataagttctc ccatgtgact tttacgtctt
     9061 ttatttatat gggattactg tttatggaaa gttgaaataa ttttcattaa agaactcttt
     9121 ctatattttt gaaagcgtag tggagcttcg aatatctggg aaggacattt tgcagggatt
     9181 gaaaaaagat attgtcctat cgaaaaatta atgaatttag acaaattgcc agcaacaggc
     9241 ttcaaactta gttgctatcc tgtcaagatc aagaaggcaa gtgcgggttg gataagagca
     9301 ggggcaatga tcgaagagaa aaagaaagaa taagccaaaa catctgtatg gaagaa
//
LOCUS       DACDKL010000099         9342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDKL010000099 DACDKL010000000
VERSION     DACDKL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.099"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            135..2606
                     /locus_tag="IV329_001829"
     CDS             135..2606
                     /locus_tag="IV329_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8941000.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="IV329_001830"
     CDS             complement(2709..4445)
                     /locus_tag="IV329_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8941001.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="IV329_001831"
     CDS             complement(4442..5146)
                     /locus_tag="IV329_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8941002.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="IV329_001832"
     CDS             5277..5780
                     /locus_tag="IV329_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8941003.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="IV329_001833"
     CDS             5740..6078
                     /locus_tag="IV329_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8941004.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="IV329_001834"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="IV329_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8941005.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="IV329_001835"
     CDS             7629..8207
                     /locus_tag="IV329_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8941006.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="IV329_001836"
     CDS             8211..8732
                     /locus_tag="IV329_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8941007.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9341)
                     /locus_tag="IV329_001837"
     CDS             complement(8811..9341)
                     /locus_tag="IV329_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8941008.1"
                     /translation="MGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLPFYNEDLET
                     PEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHSVWDHKPGL
                     VVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNIVEGTLSFF
                     QTILDAYLDFSNKLTE"
BASE COUNT         3020 a         1726 c         1956 g         2640 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa ta
//
LOCUS       DACDKL010000100         9084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDKL010000100 DACDKL010000000
VERSION     DACDKL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.100"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1234
                     /locus_tag="IV329_001838"
     CDS             <1..1234
                     /locus_tag="IV329_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8941009.1"
                     /translation="KEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAISFYRKL
                     FSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGDLVCHWF
                     TFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALPSKIGVI
                     LNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQLPEDYT
                     KEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGRRMNEYR
                     GWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYRIEYIKD
                     HLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIKKSGEFY
                     KQLSDTNGFRYDVDLLVE"
     gene            1533..2240
                     /locus_tag="IV329_001839"
     CDS             1533..2240
                     /locus_tag="IV329_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8941010.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2227..2550
                     /locus_tag="IV329_001840"
     CDS             2227..2550
                     /locus_tag="IV329_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8941011.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2639..3676)
                     /locus_tag="IV329_001841"
     CDS             complement(2639..3676)
                     /locus_tag="IV329_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8941012.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(3997..4980)
                     /locus_tag="IV329_001842"
     CDS             complement(3997..4980)
                     /locus_tag="IV329_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8941013.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5395..6165)
                     /locus_tag="IV329_001843"
     CDS             complement(5395..6165)
                     /locus_tag="IV329_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8941014.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6158..7093)
                     /locus_tag="IV329_001844"
     CDS             complement(6158..7093)
                     /locus_tag="IV329_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8941015.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7409..9058)
                     /locus_tag="IV329_001845"
     CDS             complement(7409..9058)
                     /locus_tag="IV329_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8941016.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
BASE COUNT         2646 a         1908 c         1654 g         2876 t
ORIGIN      
        1 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
       61 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
      121 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
      181 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
      241 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
      301 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
      361 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
      421 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
      481 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
      541 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
      601 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
      661 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
      721 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
      781 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
      841 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
      901 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
      961 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     1021 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     1081 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     1141 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     1201 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     1261 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     1321 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     1381 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     1441 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     1501 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     1561 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     1621 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     1681 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     1741 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     1801 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     1861 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     1921 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     1981 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     2041 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     2101 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     2161 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     2221 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     2281 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     2341 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     2401 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     2461 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     2521 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     2581 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     2641 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     2701 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     2761 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     2821 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     2881 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     2941 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     3001 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     3061 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     3121 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     3181 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     3241 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     3301 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     3361 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     3421 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     3481 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     3541 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     3601 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     3661 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     3721 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     3781 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     3841 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     3901 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     3961 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     4021 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     4081 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     4141 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     4201 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     4261 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     4321 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     4381 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     4441 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     4501 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     4561 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     4621 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     4681 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     4741 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     4801 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     4861 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     4921 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     4981 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     5041 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     5101 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     5161 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     5221 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     5281 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     5341 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     5401 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     5461 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     5521 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     5581 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     5641 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     5701 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     5761 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
     5821 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
     5881 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
     5941 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
     6001 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
     6061 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
     6121 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
     6181 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
     6241 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
     6301 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
     6361 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
     6421 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
     6481 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
     6541 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
     6601 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
     6661 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
     6721 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
     6781 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
     6841 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
     6901 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
     6961 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
     7021 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
     7081 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
     7141 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
     7201 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
     7261 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
     7321 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
     7381 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
     7441 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
     7501 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
     7561 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
     7621 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
     7681 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
     7741 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
     7801 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
     7861 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
     7921 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
     7981 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
     8041 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
     8101 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
     8161 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
     8221 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
     8281 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
     8341 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
     8401 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
     8461 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
     8521 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
     8581 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
     8641 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
     8701 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
     8761 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
     8821 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
     8881 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
     8941 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
     9001 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
     9061 ccctctccgt tcctttattt gtta
//
LOCUS       DACDKL010000101         9043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDKL010000101 DACDKL010000000
VERSION     DACDKL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.101"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..2243)
                     /locus_tag="IV329_001846"
     CDS             complement(69..2243)
                     /locus_tag="IV329_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8941017.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2259..4601)
                     /locus_tag="IV329_001847"
     CDS             complement(2259..4601)
                     /locus_tag="IV329_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941018.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4623..7166)
                     /locus_tag="IV329_001848"
     CDS             complement(4623..7166)
                     /locus_tag="IV329_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8941019.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7176..7574)
                     /locus_tag="IV329_001849"
     CDS             complement(7176..7574)
                     /locus_tag="IV329_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8941020.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7655..7939)
                     /locus_tag="IV329_001850"
     CDS             complement(7655..7939)
                     /locus_tag="IV329_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941021.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(7963..8961)
                     /locus_tag="IV329_001851"
     CDS             complement(7963..8961)
                     /locus_tag="IV329_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8941022.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2576 a         1955 c         1421 g         3091 t
ORIGIN      
        1 tagtttttat cagtttaaaa caaccaaata agaggaaact aacaataagt atcctaacaa
       61 taactttctt atttcgcata ttgtttctcc aaatttggtt tccgaatata gacgactcgc
      121 ggtggttcct ggacgtcttc agctgttcga tacaccacaa attcgcctgt tctcaatgct
      181 cgaatgcgat tcggattgaa tttaaagcgg tctacgtctc gtttcgtgcc tctttctcct
      241 gtccaatcaa cctttcccat atcagaataa cctgcttctt gtgtcgtcat ttccgttaat
      301 tcaatgtctt cttctgtccc cattaaatct gcccaataat caatttctgt cgggtcattt
      361 gtcttcccaa taaagaacgt attcacattc ccaatcaact gttttcgaag gacaggatca
      421 attctatcaa tgtccgcaag cgtttgaggg gcatacgccg catgcacccc agcgcctcgt
      481 cccttattga ctaagtctat aaggttatca tttagataag aagctggttc gtcaaatatt
      541 actaggactt tcgagcgatt ggctgtttct cctgcttgtt tatacacttc tgacgcaaag
      601 aaattaatat ctcccacaag catttgagcc aatgtccgaa taaactcact ataaatcaat
      661 ccgtttaacg agatataaac aatctttcct tctttaacaa cttcttgaat atctaattct
      721 ctttttcctc cagaagtatc aaagagttca ccaagctcac tatccaataa catattgata
      781 ttggtctgta aattctcaga agtcgcatac aaataaaacg ggttcttctt atgttcgtaa
      841 cgaacaaaca accgtttaaa caacgtttgc tcgatttctg tcatgagaga aatgttttct
      901 tttaataata gataatacgt ttctaagtca acggttttac gatcatgttc aacgattcgc
      961 tcttcactta actgttccac atcggcaaaa tcacgttctg ttttgtccat cgcttcattc
     1021 tttttcgttt ttttcttcgt ggttttcttc ttttctacga ccactttttc tttaaataaa
     1081 tctggatttt tcaactgaat acgatccgca aacaaatgta agacgtttcg tggcaataaa
     1141 taatgttgta accaaactag atttctttca attttatggc tcttttggaa ctcgtctaaa
     1201 agctgaatcg ttacctgtaa taacgctttg gctgctgtcg aataatacac gctttctgtt
     1261 tctgctagat tggttaattt atctcgaatg gcaatatcat tgccattttt tacaggatta
     1321 taacgcaaat cgccaccgtc cacaaagact tcaatcgggc gattgtatgc ctctgccact
     1381 tcttttaacg cttgtaaagt ctctcctgct cctttcccat cgactaagat aacggggata
     1441 cgaaacttac acgcatgttc tgctagtaat tggatcaccg tcgtttttcc tgaacctgta
     1501 gtcgctggaa taatcacatg ttggttcaat tcactaaagt tcatcacata agggagccca
     1561 tactcatctg ttcctaatag aatcactttg gctaatgaag ccagttgttt attccgcaaa
     1621 gcaacgtctt ttatgacaat tgctacctgg tacgcttgct gatactctgc tgtatatttt
     1681 tgggactcct gaaatttatt cttccgacgt tccttgtatt ctttactatt tagcaaagct
     1741 gtttgccgtt ctttctttga aacaactttt cttttttcaa taaaagacgc atacgcaatc
     1801 aaacaacttc ctgcacctgt cccacctaaa aggagggcaa aaatcgtttg tccattaaac
     1861 tgcaacgcat gagaaaacaa gtttaatgga aataataagt aacacgacaa ataccctcta
     1921 ggaagaacaa aaataaaaca aaatagattt aatagcgtaa aacctaacgc aaaaataaaa
     1981 aaaagagagc ttcttcgttt taagaagctc tcatactcaa agtcataaat atgtgcacgt
     2041 gtctcttttt tatctatcat aaagacaatc gctaaaggaa tactgcctac aataaaaaat
     2101 ggatatagac aaacacctaa taaagtgacg ataaaataaa gttgttgaaa ccgacttttt
     2161 ggcgtttctt tttcttgttt tcttttgcga tcgacagaaa acatttgctc aaaatgtgtc
     2221 ggtcgttttg gtgcattcat catactatcg atttcctcct atcgttttcg ttgtttcatt
     2281 tctcttcgat aaatcggttg tttttctcgt tttcgtttca tttttgcttg agttgctcga
     2341 aactcactcc gtgtcttcgg tgcttgtttt ggtgattttg tttgtttcat ggttgagtat
     2401 gctttagata cggttgaagg cgctttggtt tgtgctgtta ctcgattgac tgctgacata
     2461 tgttcccgaa ccccctctgg acgtcgaaca gatttcggtg tggtagaacc tgttgctcgt
     2521 ggtacattcc cttttcttag tggtttttgt accgcatgtt tttgtcctgc aactgtccgg
     2581 gttttcatcg aacttgtttt tgttacggga gtaagtgcct ccttccgacg acgacttgtt
     2641 tgtccctgac gacgttttcg atcgttcaat tctgttctag gcgtatgctt ctggccataa
     2701 gacttgtgtt tttgctttgg attcgctaat gacaccacca ttgcagaacc ctgattgcga
     2761 tacggactac gagacgcacc ttttctactt ctttctctta gactaggatt gccttgcaat
     2821 tctcgttgta ataaatcggc tgtttttctt gcctgtttct ccattgctgt tcgttgcata
     2881 cgttcttgac tttgctcttc tgcttgtttc gcttgatcat gcaagtctct tgctttttgt
     2941 gccatacgtt ccatacgatt tggtgcttta tgacgaggtt gttgtgcgga ttcacgccga
     3001 aaagcacttc gtgtggcttg tttcgccgtt tgtggcatac gaacgtctga gccaccccca
     3061 cgttttcgtt gacgttcttg tctcttccgt gcgttttctt tttctttttg tttctgtgct
     3121 tttctcgctt ctttgcgttc tttcaaccgt tgtttggctt cccttctcaa ttttgcttct
     3181 gccccaaatg gatttcccgc aaaagctaac gctcctcgaa cacccattct tccagtaaat
     3241 agactgccta aaaagcctcg gaacaaataa attcccaacg gcgcaaacaa ataaacaatg
     3301 atcaagagaa ttttttccca tggatcgtct tgtgtactac ttaattggaa cccgtaacta
     3361 agaaaagaag aaaaaaggat acatgcaaaa cctgtcattc cttttaaaaa cgtcaaaata
     3421 ccaaaaactt tggcatagtt accaaacaca ttcattgtga caagaaacaa tcctaaaaaa
     3481 agtaagaacg gcaacatcgg tactaacaat agttgcatag ttccaataat caatcgaaac
     3541 atacttaaga caaaataaac aacggtttga atcagattca cgacaaaata aaacaaggca
     3601 aatgctgtat ttttccaact attgtaatat tgtatgttct tgtttttcag tgtatctgct
     3661 tcatactctg tgactttgtc atgaattttc tcccctattt ttgtacctaa atcattgtcc
     3721 aataaaagat tgattcgatc atattctttt ccagcatatt tgactttctt ctcacgaatt
     3781 gtgtctacat tggttgtgcc ataatttaac aacagatatg gttcataaac attactataa
     3841 aagacccgag ccgccatcaa tttgcctgcc gtttccactt ttttatccgc tgttacctct
     3901 tctgtttctt ttgtttcata atttgtttct tcttcaggtg ttaacactgg attgaccttc
     3961 acgaacgccg tttccactgt agaatctaaa gaaaaaagaa catcaaaaag attcttattt
     4021 gtcccagaat cttttaatac gcttaaccca gtaaaaataa ggatcgtcat aagaaagaca
     4081 cggagtgctt gtttcattcg agcctctcca gcatatttga ttgccatcat aattgccgaa
     4141 aaactgattc caatagttcc tgcaatactt aacatattgt ccgcaatttt ttgtgttaag
     4201 ttttgaatgg gttctcgaat ggcggaaata atatctaatt caaagagaaa ttcaaccatt
     4261 gaagcattga attctcctaa catttttacg ccactccaaa caaaattttt catcactcct
     4321 gcaacattgt acgatagctt ttttttaagg ttcattgact catagtcatt ggtcatcaat
     4381 tcaaacgaat tctttttgta atttttatac aattgatcca catcagtcgt tacttctttt
     4441 acttctactt tatcgttggc attctctcct tcagaacgat cggtttcagt gacacccgca
     4501 ccatcattca tttgatcggc agaaacagta gccccttgta gacagaaaaa aagaccccct
     4561 aataaaagga gcgtccacca tttcagagat ttttttcgca aaaaaaccac ctcttttttg
     4621 ttttatcgtt gtttttcatt ttctaacatt gcttgaattt tttcatcttg attttcagta
     4681 gaagaaacag ccaataacca actatcaaac aacacatcaa tggcaatcgg ttgatttctt
     4741 cccaaatgat cctggaataa acaggttcct gtttgtaatt gttccatcat ttctaaattg
     4801 gctgaattca cttccatatc aaagaaggac aatagtttct ctcgtgcttc tttttgtttc
     4861 ggacgaaacg caaatttgta agaaattaac tcttttttat cttcttgatc gtaatcttta
     4921 aacgcttgca caacaaggta aatatccgta ttattcgcac gtccttttcg caagctatca
     4981 tcaatcaaat agcttccttg tgcggtatct tcataccctt ttgcctcgtc ataaataatc
     5041 gcagcgtctt cttctttgtt tgtagaaaag atatacgtaa acttcataat gacttccata
     5101 attgccattc ctgcaagttg ttcgctattt aatcgacccg aatcaatctc tttttgtgtc
     5161 ggaatgcgta ttccttgtgt ccctaacacg ttgatttgtg aatcaaaacg tatcggcgta
     5221 ctgtgctcat tcccaatgag aatctttcct aaaccaacgc tatgccctaa aatcaattta
     5281 gctagttctt tgtcactttt tcgaatttct tcgataactt gacttaacga atgcttacct
     5341 ggcatggtgt aaactacatc gatggcgtct tggatcaccg ttttcttgtg gctcgctgtt
     5401 tctcgattgg tatctacttc tcctaacatt cctaacacat ttttagcggt ttgaattgcc
     5461 tcttcttttg gtaaaaacaa tagaggatca agcatccctc gacagctttc atctgaagat
     5521 aacgaaataa aattaattcg ttcatacata gccacaaact ctggtatgtg tccgaatttt
     5581 tcaagtgcct ttaaaaagaa cttttgggtt tcatttttcg gatcaatata aagaattttt
     5641 tgacctaaaa acatcagcca taaaaaaata tattttacaa ggaccgattt tcctttacct
     5701 ggtggaccaa taatcaacgt atttccattg gtatgttccg cgccttcaat acttttcttt
     5761 gttaaattgg gataatacca tactagttta ctagatagcg ctaacgcttc tttcgcagat
     5821 ttaaactttt ttgcaataac aatccgtcct aaaggaaatc ctttcttatt tcctacttct
     5881 ttctttaagt ccaagccata atctgccaca aaacctgtcg tagcgacctg ctcgtaacta
     5941 cgaaaacgtt gcgtacttcc aattaagcat tgattaaaca acgttagctg atccgctacc
     6001 gatcgataaa atttgaaatc tgtagactca atcgcaaatt caatatcttt gactcgttct
     6061 tccaattctt cttttgaaga agcagataca accagcgtga cggtagaata acacaatcgt
     6121 ttttctttgc gcttcagctt atcttttaca tccattaacc gttctgtacc aaaatttaat
     6181 acatcgtctg aatctaaaat gccgtcaacg gtctcatatt ctttttcttg ttcataaatc
     6241 cgtccagcca ttttattaat ttttcgttca tcagattttg aatgctcaaa tcgaagtcga
     6301 atatgcgtat caattggaaa agataagcta tcctgaagat tttgaataaa gctactccca
     6361 tacatggcta aaggagcgtc cgtttgactt aaaaacgtcg aataatgcgt ttgattcaac
     6421 tgttcaatcg caatgtatcc tgcttcattc gaaatgacgc cctcgcaaat ttccattggc
     6481 gtaagctgtc tttcaggcaa tttttcttct gatcgatgga aaaaataata ataaatttgt
     6541 ccaatctctt gggcagataa gcgctctatc ggcttataat tggacaattc cgtatagagc
     6601 tccttttcta atgtttcata cgttggtaac acactactta taggaactcg ttgtcccgta
     6661 atacgataga taaacttttt aaaaacatgc ttaagcaatt gaaaatattc gatcggatca
     6721 gacactttca tttctgtagt gaaacgcaca aacagatacg ttcgatactc gtttaattgc
     6781 acttcgtctt ttaaaatctt tcctgcctgt tcaaagtaca ctttcccaag atctgcgcat
     6841 tctcccttta ccaaatctcg aatggtttct tcaatgtgcg tatctaggtt aaattgacgt
     6901 ggaatattga taaaatgata gtcatattca tcatgtccaa gtatgccttc gccatcttga
     6961 agatagttct tgaaaaaatc aatgtcattt aaaggaaata cttgggaaga aagttgatag
     7021 cctacccaca catcatcatt tttggtcaaa acaaaatttc ggataatggc gtctatcgga
     7081 aattctaatc gaggaagctt cgtttttttc tttttgaact gccaaatccc gataataaag
     7141 acgaccaaaa gaaacaaaaa cgccatttac tcgactcact ccttccattt gtgtagggaa
     7201 tacgtcactt tttcatggaa aaacggcaca tacatttcat gttcaaagcg ttgtttccgt
     7261 ttctgataat aggacgttcg cccaattaaa tactcgaaaa agtgtttatt gtcccatttc
     7321 attgaaaaca atgtccaaac gagaacacca atcccaaaaa tcataatcaa ccaggacttt
     7381 cgaaacagtg tctgcaagaa actaatttga ttcacatacg caatcaaaaa taggatcaaa
     7441 agaatgaaga gaaatacccc acctatgact aattgttcca tgcgaatgcc attagaaatc
     7501 cagggaattt tttgtccttt aatcccgtaa attttgtgag ggtgcttaaa ctcctttgta
     7561 tagttgtatt ccatgttcac tctacctcct tttacaaaaa aagaagcgac cagaagttct
     7621 aatcttctga tcgcttttgc agtttgtctt tttcttatga taattgtatc cagggcacaa
     7681 tcatttgcgt tacccccacg ttctcttcac tatccttaat aaagataatg ttctttagcc
     7741 attcaccaat atcaacggca ttcgataaca ataagacgag cattatcaaa ataaagacgc
     7801 caataatggc aaacgctgcc cgcgcaaagc cttgggtcgc ataagcacca accattagaa
     7861 caatcgcaac gattgccaat agccaaatga cttgtccaga tacatcatca aataacgatt
     7921 taagatcaaa aagtttcatt tccattctcc tctaagttca ttttattcat ttaacgtagt
     7981 aacaaaataa ctatcgccac ttgtttttac ttccattgaa aagtttgatt ggaactcgtt
     8041 tcctttctca aaatagaaac gatacttccc ttgcaaaaag tagtttcctt ctttattttt
     8101 tactgcatga tccagttgta cattgatcaa ttcgccgcct tctaaccctt tttcgttaga
     8161 aatcaatgcc aatttttcat catttttgac atataactca aaaaaccgtt tcacaaatgc
     8221 ttccagcttg atttcttcgc tttcagtgac cgcttgccca gaagaataaa atcgattttc
     8281 ctgatagttc acagaattgg tattcttact ctcagaaaga ttcaaaaatt gaacgccatc
     8341 aagtactttt aaaacattgt tttgataact gactggaaaa gaaagttgac tgcttttcca
     8401 atgttctcct tctgtaaaag taacctcata aaataggcga tacattgctt gtgtcaaatc
     8461 gatttcttct agtttaataa agcgaacatc tttcgctact cgattggtag ccgtcgttac
     8521 aaagcctaga tcagacgctt ccaatccttg tgctaaataa tgattgacgg aagcctccca
     8581 ttcgtttcgt ttttctggcg aataagtaaa taacctttca ataaaatgtt gtccctcgta
     8641 cttcaattgt tccgtagaag acaaggtttg gttgacttca gttaacaatg tttctggatt
     8701 gacttgtttt ttcactgcta aacgcgttaa tgtatccact cgtccaaagt ttaggaggag
     8761 cacgacaccc attaacagaa acaaacaaaa aaaagcgcct gaaaatacac tcatggcact
     8821 tttttgcgaa aattggatca ttcgatctga cggttctgtt ttcttttttt ggaacaaaaa
     8881 acgtttcttt tgtttctttt ccttctgttg tttcggtggc ttagtttgga acgcttgtgg
     8941 attaaatttt gcattactca tgagaaatgc cataccgttc taacaataat ttcggtattt
     9001 tgtccacaat cactacttcg tggacaacca cacttgcccc ctc
//
LOCUS       DACDKL010000102         8979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDKL010000102 DACDKL010000000
VERSION     DACDKL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.102"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..881
                     /gene="gnd"
                     /locus_tag="IV329_001852"
     CDS             <1..881
                     /gene="gnd"
                     /locus_tag="IV329_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8941023.1"
                     /translation="GKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAYQEISE
                     FLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRAEEFQK
                     NEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGASGSGH
                     FLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEIMEDQL
                     NEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFSAKVVS
                     ALRNGFGGHEIVEKGE"
     gene            883..1884
                     /locus_tag="IV329_001853"
     CDS             883..1884
                     /locus_tag="IV329_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8941024.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1881..2165
                     /locus_tag="IV329_001854"
     CDS             1881..2165
                     /locus_tag="IV329_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8941025.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2198..2995
                     /locus_tag="IV329_001855"
     CDS             2198..2995
                     /locus_tag="IV329_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8941026.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3009..3842
                     /locus_tag="IV329_001856"
     CDS             3009..3842
                     /locus_tag="IV329_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8941027.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3860..4717
                     /locus_tag="IV329_001857"
     CDS             3860..4717
                     /locus_tag="IV329_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8941028.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4739..5248
                     /locus_tag="IV329_001858"
     CDS             4739..5248
                     /locus_tag="IV329_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8941029.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5285..5686
                     /locus_tag="IV329_001859"
     CDS             5285..5686
                     /locus_tag="IV329_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941030.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5798..6151
                     /locus_tag="IV329_001860"
     CDS             5798..6151
                     /locus_tag="IV329_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941031.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6185..6628
                     /locus_tag="IV329_001861"
     CDS             6185..6628
                     /locus_tag="IV329_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8941032.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6645..7730
                     /gene="uxuA"
                     /locus_tag="IV329_001862"
     CDS             6645..7730
                     /gene="uxuA"
                     /locus_tag="IV329_001862"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8941033.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7723..8436
                     /locus_tag="IV329_001863"
     CDS             7723..8436
                     /locus_tag="IV329_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8941034.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3006 a         1424 c         2011 g         2538 t
ORIGIN      
        1 tgggaaaaat gggattgaat ttagctttaa acttaaaaga gcaccatcac aaagtacaag
       61 gactcgacat ttcaagtaca gctgttagtc aggcaagaga aaatgacatt cctgcttatc
      121 aagagatctc tgaatttcta gctacttttg agcagaaaaa aatcatctgg ttaatgcttc
      181 ctgctggaga gataaccgaa aacatgttaa gaaaactttt tccaaaactc ggaccaggag
      241 acattattat tgaaggcgga aactcttatt ataaagattc aatccgtcga gcagaagagt
      301 ttcagaaaaa tgaaatcggt tacttggatt gtgggacttc tggaggaatc gaaggagcac
      361 gacatcaagc ctgcttaatg attggaggag ataaagaaac ttttctatcc gtggaacaat
      421 tattcaaaga tgtagcaatt gaaagaggat atctgtatgc tggtgcatcc ggaagcggac
      481 acttcttgaa aatgatccat aatggaatcg aatatggaat gatgcaagca atcggtgaag
      541 gattccaact tgtacaaaaa agtgagtatg cttttgactt ggaacaagtc gcacgtgttt
      601 ggaatcatgg ttcggtgatt cgcggctggc tgatggaaat catggaagat cagttaaacg
      661 aacatcccaa tttaagtaat tatcggggaa tcgtttctgc atcaggtgaa gcaaaatgga
      721 cggttgaaac agcgctagaa atggctgttc cagttccagt aattgcaact agtttgttca
      781 tgaggaatct ttcacaggaa gaagatagtt tttcagcaaa agtagtttca gcactaagaa
      841 atgggtttgg cggacatgaa atagttgaaa agggtgagta aaatgccaga ttatcatata
      901 gcaatcgtta attccagcag tttcggaaga gtctttccac accacttgga aagattaaag
      961 tcagttggag acgttcattt ctttactgtt gatcaagaga ttccaggaaa agaactagcg
     1021 gagttactta aaggattcaa tatcattatt gcaagtgtta cgccattttt tcgacgagat
     1081 ttttttgaaa acaaagatga actgctattg atttcccgac atggtattgg ttacaacaat
     1141 attgatttaa aagcagcaaa agaacaccag acaatcgtat ctatcatacc tgctttagtt
     1201 gaacgaaatg ctgtagcaga aaataatata actaatttgt tgtccatgct tcgacgcacg
     1261 acacaagcac aagcaagagt caaggacgat cgatgggaag atcgggcgga gttcgtcggt
     1321 aggacgctgt ttaacaaaac agttggtgtg attggtgcag gaaataccgg aagcggagta
     1381 atcgaaattt tgcgtaacgg ttttcgctgt gatgttctag cttatgatcc ttacaaatca
     1441 gatttataca tcgaaagctt tggtgcaaaa aaagtagaac ttcaagaatt actggaacaa
     1501 tcagatatca tttgtttatg tgcgaatttg acagaagaga actatcacat gatttctgat
     1561 aaagaagtcg atcagatgaa agaaaatgtt tatctatcta atagtgcaag aggcgctcta
     1621 gttgatgaag aagcgatgat taatggtctg aaaagcggaa agatcgcagg atatgccaca
     1681 gatgttttag aagaagaacc gggacgtcaa gatcatccgt atttagcgtt tgaacaagta
     1741 gtgattacgc cacatatatc ggcctacaca agggaatgtt tagaagcaat gggtgaaaaa
     1801 tgtgtcagag atgtcgaaca gatcagtcag caccttcttc ctgaacgttc tgtccagaca
     1861 aagagcaact atataaaagg atgatgaaga aaatggtgaa cggtttaaaa gtgaaaacag
     1921 gccctcaatt ttatttatat gaagaaggtg gtatttcaaa agtttctgat ctcttgaaat
     1981 catacggcgc gaaaagagta ctggtcaccc acggaacggt atcatgggaa aaggcgctac
     2041 ctaaattagt atttttgaat gacgaaacga ttcaattttt ttatcatcgt tattctggag
     2101 aatgtagtta tgctgaagcc agacgaatag ctacaatcat caaaaaaatg aaatcgattt
     2161 cgtagttggc gttggcggag gaaaactgac cgatctagtg ggttatagtg ccaatcttgc
     2221 aagaatcgat ttcggagtca tccctacttt agctagtaat tgtgctccat ggacacctct
     2281 tgctgtcatg tataaggaga atggacaagc agaagggaaa acagaacact tttacagaca
     2341 agctgctttt tttataacag atccccaatt agtgattgat tcccctgttc catatttcat
     2401 tgccgggtta gcagatacac tagcaaaatg gtatgaatct gagctcatct tagaacaaga
     2461 ttttttgcaa gatgaatcat ttttgaagct agcacaagat actgcgaaga tctgcaaaga
     2521 agagattttg aatcattctg ctaaagcaat ccaagatatg gcacaaagaa aactgacatc
     2581 ggagttcaaa cagctttcag agattatctt tgctgttgct ggtttagttg gaggattagg
     2641 agataaatac gcaagaaatg tggcagcaca tgctatgcat gacgctatca gtaaatatat
     2701 tccggaaagc catcgttttc tacatggaga aaaagtagcg tatgggattt tctatcaact
     2761 ggcactagaa ggtcgctggg aaaaaataga cgagttatat cctttgtatc gggaattaga
     2821 actgccggtt tctctagaac aaatgggatt atttccgaaa gataaacaga taatagataa
     2881 aatggctgaa tttattgatt cgaaagagaa agtgcatttg attcctatag aggtaacaca
     2941 gaaatcactg atacagtcaa tgatggaact ggaaggttat atcagaaaga tctgaaggag
     3001 gaggacacat ggaaacatta agtgtagtac aaagtttgtt gattgcttta tgggtagccg
     3061 cggttatgtc ccgctggtta ggtggaggag ccacgttgac cttacgtttt tctcctttga
     3121 tgactggatt agtcgtcggg ttgatcatgg gggaagttgc acaagcgatg attgttacag
     3181 ccgctttgca aatgatttat atgggggttt tttctccagg aggatcaatg ccagctgaac
     3241 catctattgc agcagctatc gcagtaccag tcgcattatt aggggatctg tcgccagaag
     3301 cagcgattgc cgttgcggtg ccagtcggat tgctcggaag ttacttgtat cagtttcgtt
     3361 ttttcatcaa tactttttta ggtaaatata cagacaaagc tgtagaagat ttgaatacaa
     3421 aagctatggc ccgatcaatt attctttatc caacggttgc ctcattttta ttattcgttc
     3481 cgcttgtttt cattgcttta tatttagggg cgccattgat cgctgatgta atcaaagctt
     3541 tagagggaac agtcgttatc cacattcttg aagtagtagg tggagggtta gcggcaatcg
     3601 gtatcgcgac aactgtatac gttatcggtc gaaaagatta tctggtcttt ttcttccttg
     3661 cttattttat gagtgttgtt ttgaaatctt tagatattac gatggttact tatgccattt
     3721 ttgggattat tatcgctttg atctttgtcc agtcgcaaaa agggaaacaa gtacagcctg
     3781 ccacaagcaa taacaattca gcatcttttg atgacgaaga tgactatgat gacggatttt
     3841 aatttaggag gaatgggaaa tggctgaaat aatggataat caaacaggtg ctcctgaaga
     3901 aatcacgaaa aaagatgtaa caaaagccta tctgagatgg cattttgcaa acgaaattcc
     3961 tcactctttt gaacgttact tggcgccatc actactatac ggaatgatgc caatcttgaa
     4021 aaaactttat aaagatgaag actctttaaa agcagcatat aaacggcagt tactgttctt
     4081 caacactcaa ttaagctggg gtggcggtgt gattactggc ctaatgtctt ctatggaaca
     4141 gcaaagagca gaagaagagt atgaagaaaa agagatcttg atgcaggatg acttgatgta
     4201 taacacgaaa gcgggattga tgggggcact agcaggaatc ggagatgcaa tcgattcagg
     4261 aaccgtccaa tatattttta tcgcaatcgc ggtaccttgg gcacaacagg gaagtccttt
     4321 aggcgcactt tttcctttta tctgctttgg gttgtatcag gttttactag gggtattttt
     4381 cgcaagacaa gctttcacta tgggccgtaa tgctacaggc ctaaagcaaa acactggtgt
     4441 tcagacagcg attgaaatgc tatcagtttt agggttgttt atgatggggg tgcttgcggg
     4501 aaactatgta aaagtagaat cttcattgaa atttgcgata tccggacgtg agtttgttat
     4561 ccaggaaatt cttgatcaaa tcgtgccagg gatattacct ttagctgtcg taatgggtgt
     4621 gtacttgttc tatacgaaaa aaggattgaa agtcacacaa gcattattat ggctgactgg
     4681 aatattaatt gtgctagcag gaatcggtat tttataaaaa gaagggaacg tgaataagat
     4741 gggtgtagtc aatttagcgc gtgtggatga gcgtttgatc catgggcaag taatggtaac
     4801 gttatctcaa aaaagtggag tgaattccat ttttgttgta gacgaagttg tagcaaaaga
     4861 taaatttatg agagatcttt acaaaaatgc aggaagccgt acaggacaaa aaacaattgt
     4921 cattacacca gagaaagcta aattctattg ggatgaatat caatttaaag aatacaattg
     4981 tattttgatc gcaaaaacag tccaaacgat ttacgactta gtaaaacacg gaatccccat
     5041 ggaagaattg aatattggag ggattgctca aaaaaatcct gatacagatc ttttagtgac
     5101 gaaatctgtt tatttaaata aatcagatgc tgaaaaattg aaagaaatga atgaacaata
     5161 tggcgtaaag aatatttatt ttcaagccac tccatctgca cctaaaacag gattaaaaga
     5221 tgtattgaac aagtttgatt tgtcataaac aaatgcttag aaaaaagata aagagggcga
     5281 aaaaatgaag aagaacgagc tgtttcgtga ttgggaattt cgttatcgct atatttaccg
     5341 taagcgccgt acaaaaaaga gtaaacagcg ttttctttca gcattagtat cagatattta
     5401 cagtatgaga acagatgtga cagtcattgc ttacgatacg ctagcctacc gatcaaaaaa
     5461 tatttatgtt ggagatatcg aaaaagcaga gaaagttatc tgtacttatt acgatacacc
     5521 cgttcatgca ttaggaagtt atttcatgtt tgattggaaa gaccaaagga aaaaaacaat
     5581 ctactctata ttattgtcct tcattttgtt gttcagttta ggttggtggg gaatgatgat
     5641 atataacaaa aatcctcatc acgtattcga tctgctatcc gtataaacag gtatcactgt
     5701 actagcattt gggagctact ttttcttatt gggaaaagca gctcgcggat ggcctagcag
     5761 acaaactttt attcgaaata cttcatccat attaacgatg ctagaaatga tccgtacgat
     5821 tgatgatccg agtgtagcgt atgcgtttgt tgatgaaggc tgttatggag aaaagggctt
     5881 agacagcgtg agatcgggta tgaaaaaaga ggctattctt ttttatctag actctgttgg
     5941 ggcagataca ccgctacagt tttcgggaaa ttatttttca aataaagaac agtggctgaa
     6001 acaagttgat aagttgaaag aaaaaaacgt caattatatt tttagtgcaa gaaaaaagca
     6061 agcgcagttc ttctatttga ccaaaacaga tttacgaggg aaaacattta attggcaaaa
     6121 tgccaatcag attattgctc tttttcgata agtaaaaagt gcaaaacaag gagtgagaag
     6181 actaatgttg gaaatcatga tcgttacaca tggtaagttg agcgaaggac tgaaagatgc
     6241 ggcggaagta attgtcggga catcgagcca tattgaaaca atgtgcttag agtctgggga
     6301 ggacatccaa acattcggag agaaggtcaa agaaaaatta actatgatca acacagcaga
     6361 acccatgcta gtatttgttg atctaattag cgcaagtccg tacaatcaag cgctcttggc
     6421 agtcaatgaa ctgactgaag aaaagaaagc agccacttat gtcatagggg gcgtgaatct
     6481 gccaatgttg ttagaagcaa tcaatcatca gatgcttcac acaccgatag aagaagcggt
     6541 ggaacaaatt gtcaaacaag gaaaagataa tttaactgtt tggcatgctt cagcggaaga
     6601 aaaaaaatta gatgaggacg atttttagaa ggaaggctga aaagatgaag tggggattta
     6661 gatggtatgg tgaaaaaggg gattcgatcc cattaaaata tgttcgccaa ataccgggaa
     6721 taaatgggat aattggaact ttgctgacta aaatgcctgg agatgtttgg gagatttcag
     6781 aaattgagga gttgaaagca tctgttgaga gccaaggact ggaactgctt ggaattgaaa
     6841 gtgtagcgat ccacgatgca atcaaggcag ggacagatga acgagattac tatattgata
     6901 actatatacg aacaatcaaa aacttgtctc gatgtggggt tcgattgatt tgttatagct
     6961 tcaaaccaat ttttggatgg gcgaaaactg atttgtttaa tgaaaacaac gatggaagct
     7021 attctctttt atacgatcag aaaatcgtcg atcagatgga gccaggagaa atgtatcaat
     7081 tgatacacag ccagtcaaag ggattcaaat tgccgggttg ggaagaagaa cggttgaaaa
     7141 aattcgctca gctggaaaaa ctgtatgaag gaatgaccga agaagaatta tttgaaaatc
     7201 tgacttattt tttagaaaaa gtgattccag tgtgcgaaaa agcagatgta aaaatggcta
     7261 tccatccaga tgatccgcca tgggaacttt ttggttttcc tagagtgacg aaaaatcttg
     7321 gcgacttgaa gaaaattcta aatgcagtag attctccata taatggacta acgctgtgca
     7381 caggatcttt aggggcaaat ccggaaaacg atttagttga aatcatccat gaggtaggtt
     7441 cgcgcatcca ttttgtccac ttcagaaacg tggagttttt aggagaaaga aagttcaaag
     7501 aaacggcaca tccctctaca gaaggctccc ttgatatgta tcatattatg aaagcgttgg
     7561 tgcaagaagg gttcgacggt cctatacgcc ctgatcatgg tagaaccgtg tggggtgaag
     7621 tggctatgcc tggatatggc ctgtatgatc gtgcaatggg actcacttac atgcaaggtc
     7681 tatatgaggc gataagtaag gaaaaaccac tgggagagca ttatgaataa aatcaagctc
     7741 ttcatctttg atatggatgg ccttttatta gaaactggac gtttagctta tcgtgcttat
     7801 gtaaaatcag cacaaaagta tgattatgaa atgcgtaagg aagtctatta tttactaact
     7861 ggccaaacag aaatggcgat aagagaacaa atgggcattt tgtacggaga agatgttcca
     7921 tatatcaaat ggcgagaagc aatcaatcaa tataaagaaa aaatcgtgaa agaagataag
     7981 agagtatata cgaaaaaagg agcagaagaa attttatctt ttgcaaaaga aagaggaatc
     8041 catacaatcg tggcttcgtc taatacacgg gtaaaagtag aaatgtattt aaaaatggaa
     8101 aatctatatg ccttgtttga tcaaatcatt tcaggggatg atgtaaaaaa aggaaagccg
     8161 gaaccagaga tctttttgaa agcttgttca aaaatgaata ttcctccaag tgaagcactt
     8221 gtatttgaag actcgattgc agggatcgaa gcagcaaggc gagcgggcat tttgtctttt
     8281 ctcatcaaag atcatttgat agggttgccc gatcataatg ggaagcataa gttgaaagta
     8341 gatgtaagtt tatatgaatc caataaagca gactatgaat tttatgacct gcatcaagca
     8401 aaacgatttt tagaaagtaa agggttgtat ctctaataaa gaggactatt ttctagtcct
     8461 ctttatttat tgtcctgcac gactgaggga acagatgaaa aagaagtaat tttttatttt
     8521 gaagtatcta ccttatttgt agtgaacaat ttagaacaaa cacaaaacgc atctactgtt
     8581 atctaccagt agatgcgttt aaagagaaga acgagaatcg tccatatgag ggtttccact
     8641 ccttatgcag atttttttaa ttcttgcact gcttgttcga tcgtaacacc tgtcgctaca
     8701 tgattggtat tttctttgtc aaaaacgaca aatagattca aatctgtatc caaggttact
     8761 cgatattgta tatctttgtt atagaatgtc atagccattc tcctttcttt aaatacttgc
     8821 tgaaaggcaa cgaacgcgtt gataacgaaa ttccatttat aagtatacca ccaattgaaa
     8881 ggttttcata gttaagaaat tttaaagtca aaagttgcag ataaaagcaa ataaacaaga
     8941 aaacaaaaag tgaatcaatc gcggttattc aactttttc
//
LOCUS       DACDKL010000103         8862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDKL010000103 DACDKL010000000
VERSION     DACDKL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.103"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV329_001864"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV329_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8941035.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV329_001865"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV329_001865"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8941036.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IV329_001866"
     CDS             complement(2169..2906)
                     /locus_tag="IV329_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8941037.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IV329_001867"
     CDS             complement(2997..3524)
                     /locus_tag="IV329_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8941038.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IV329_001868"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IV329_001868"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8941039.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IV329_001869"
     CDS             complement(4401..4886)
                     /locus_tag="IV329_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8941040.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IV329_001870"
     CDS             complement(5039..5236)
                     /locus_tag="IV329_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941041.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV329_001871"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV329_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8941042.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV329_001872"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV329_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8941043.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
BASE COUNT         2496 a         1971 c         1485 g         2910 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aa
//
LOCUS       DACDKL010000104         8841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDKL010000104 DACDKL010000000
VERSION     DACDKL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.104"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(248..2629)
                     /locus_tag="IV329_001873"
     CDS             complement(248..2629)
                     /locus_tag="IV329_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8941044.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2619..3266)
                     /gene="recU"
                     /locus_tag="IV329_001874"
     CDS             complement(2619..3266)
                     /gene="recU"
                     /locus_tag="IV329_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8941045.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3330..3869
                     /locus_tag="IV329_001875"
     CDS             3330..3869
                     /locus_tag="IV329_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8941046.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3959..4378
                     /gene="gpsB"
                     /locus_tag="IV329_001876"
     CDS             3959..4378
                     /gene="gpsB"
                     /locus_tag="IV329_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8941047.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4400..4767
                     /gene="rnpB"
                     /locus_tag="IV329_001877"
     ncRNA           4400..4767
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV329_001877"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4976..6142
                     /locus_tag="IV329_001878"
     CDS             4976..6142
                     /locus_tag="IV329_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8941048.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6188..7684
                     /locus_tag="IV329_001879"
     CDS             6188..7684
                     /locus_tag="IV329_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8941049.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7765..8448
                     /locus_tag="IV329_001880"
     CDS             7765..8448
                     /locus_tag="IV329_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8941050.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2797 a         1589 c         1867 g         2588 t
ORIGIN      
        1 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
       61 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      121 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      181 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      241 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      301 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      361 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      421 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      481 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      541 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      601 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      661 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      721 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      781 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      841 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      901 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      961 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1021 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1081 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1141 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1201 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1261 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1321 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1381 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1441 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1501 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1561 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1621 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1681 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1741 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1801 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1861 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1921 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1981 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2041 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2101 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2161 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2221 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2281 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2341 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2401 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2461 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2521 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2581 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2641 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2701 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2761 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2821 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2881 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2941 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3001 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3061 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3121 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3181 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3241 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3301 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3361 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3421 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3481 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3541 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3601 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3661 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3721 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3781 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3841 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3901 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3961 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4021 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     4081 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4141 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4201 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4261 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4321 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4381 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4441 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4501 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4561 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4621 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4681 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4741 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4801 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4861 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4921 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4981 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5041 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5101 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5161 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5221 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5281 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5341 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5401 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5461 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5521 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5581 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5641 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5701 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5761 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5821 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5881 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5941 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6001 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6061 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6121 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6181 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6241 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6301 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6361 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6421 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6481 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6541 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6601 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6661 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6721 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6781 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6841 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6901 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6961 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7021 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7081 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7141 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7201 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7261 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7321 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7381 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7441 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7501 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7561 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7621 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7681 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7741 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7801 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7861 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7921 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7981 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8041 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8101 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8161 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8221 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8281 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8341 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8401 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8461 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8521 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8581 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8641 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8701 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8761 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8821 tacaattgat tttaacgaaa g
//
LOCUS       DACDKL010000105         8693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDKL010000105 DACDKL010000000
VERSION     DACDKL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.105"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IV329_001881"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IV329_001881"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8941051.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IV329_001882"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IV329_001882"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8941052.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IV329_001883"
     CDS             complement(3191..4432)
                     /locus_tag="IV329_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8941053.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IV329_001884"
     CDS             complement(4450..5373)
                     /locus_tag="IV329_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8941054.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IV329_001885"
     CDS             complement(5594..8086)
                     /locus_tag="IV329_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8941055.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IV329_001886"
     CDS             complement(8093..8560)
                     /locus_tag="IV329_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8941056.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2392 a         1831 c         1446 g         3024 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DACDKL010000106         8499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDKL010000106 DACDKL010000000
VERSION     DACDKL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.106"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IV329_001887"
     CDS             94..1248
                     /locus_tag="IV329_001887"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8941057.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IV329_001888"
     CDS             complement(1270..2022)
                     /locus_tag="IV329_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8941058.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IV329_001889"
     CDS             complement(2386..5052)
                     /locus_tag="IV329_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8941059.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IV329_001890"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IV329_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8941060.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV329_001891"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IV329_001891"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8941061.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8282..>8499
                     /locus_tag="IV329_001892"
     CDS             8282..>8499
                     /locus_tag="IV329_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941062.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMK"
BASE COUNT         2517 a         1745 c         1502 g         2735 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8221 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8281 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8341 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8401 cacagaaaaa ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat
     8461 gtttactgaa aaaaaagaat ttcataaaca gatgaaaaa
//
LOCUS       DACDKL010000107         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDKL010000107 DACDKL010000000
VERSION     DACDKL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.107"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IV329_001893"
     CDS             complement(<1..173)
                     /locus_tag="IV329_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941063.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IV329_001894"
     CDS             complement(593..1714)
                     /locus_tag="IV329_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8941064.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IV329_001895"
     CDS             2042..3451
                     /locus_tag="IV329_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8941065.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IV329_001896"
     CDS             3448..4176
                     /locus_tag="IV329_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8941066.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IV329_001897"
     CDS             complement(4304..5215)
                     /locus_tag="IV329_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8941067.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IV329_001898"
     CDS             complement(5232..6239)
                     /locus_tag="IV329_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8941068.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IV329_001899"
     CDS             complement(6236..8365)
                     /locus_tag="IV329_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8941069.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IV329_001900"
     CDS             complement(8370..8477)
                     /locus_tag="IV329_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941070.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2598 a         1728 c         1487 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga gacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDKL010000108         8420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDKL010000108 DACDKL010000000
VERSION     DACDKL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.108"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..2709
                     /gene="clpB"
                     /locus_tag="IV329_001901"
     CDS             100..2709
                     /gene="clpB"
                     /locus_tag="IV329_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8941071.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2860..3531)
                     /locus_tag="IV329_001902"
     CDS             complement(2860..3531)
                     /locus_tag="IV329_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941072.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(3524..4018)
                     /locus_tag="IV329_001903"
     CDS             complement(3524..4018)
                     /locus_tag="IV329_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8941073.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4038..5258)
                     /locus_tag="IV329_001904"
     CDS             complement(4038..5258)
                     /locus_tag="IV329_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941074.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5405..7240
                     /gene="lepA"
                     /locus_tag="IV329_001905"
     CDS             5405..7240
                     /gene="lepA"
                     /locus_tag="IV329_001905"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8941075.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7334..8416)
                     /locus_tag="IV329_001906"
     CDS             complement(7334..8416)
                     /locus_tag="IV329_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8941076.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2703 a         1613 c         1694 g         2410 t
ORIGIN      
        1 tgtgtatatt ttgaccaaaa ttgaccaaag cgatataata gtttttgtaa agatcaaaga
       61 aataagtgat cttaccttta attaggaggg tatacctgta tgaatattga gaaaatgact
      121 acgacgcttc aagaagcgat tgctgaagct caacagattg cggtcactcg aaaacatcaa
      181 gatatcgaca ttgcccatgt atggaaaatc tttttacaac ctaatcattt tggtcgaaat
      241 ttctatacgg atgccggatt ggatgtcgaa tcttttgaac atgaaattga tcgtctactg
      301 gatgagtatc cagttgtttc tggtggaaac gtccaatatg gacaaaacct cagccagaat
      361 ttctttcgat tattgaatga agcagaccaa atacgtgaaa gcttcggcga cgagtttctt
      421 tcaacggaag tcgttatttt agcgttgatg aaattaaaaa attatccgtt aactgtttat
      481 ttgaataaaa acggattatc tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga
      541 ggagaaagag tgacctcaca aaaccaagaa gaacaataca aagcattaga aaaatacggc
      601 gttgatttag tacagcaagt caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa
      661 gaaatccgtg atgtcatccg tattctttca cgaaaaacaa aaaataatcc tgttctgata
      721 ggagaacccg gtgtagggaa aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc
      781 aaagatgtgc ctgaaaattt aaaagataaa acgatttttt cattagatat gggtgcattg
      841 atcgctggag caaaattccg tggtgaattt gaagaaagac taaaagcagt cttgaaagaa
      901 gtcaaaaaaa gtgatggacg tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg
      961 gcaggaaaaa cagaaggaag tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc
     1021 ggtgaattac atttgatcgg tgccacaact ttagatgagt accgtcaata tatggaaaaa
     1081 gacaaagcgt tggaacgtcg ctttcaaaag gtattagtca aagaaccaac agtggaagat
     1141 acgatcagta ttctgcgtgg attaaaagaa cgttttgaga tccaccatgg tgttaacatc
     1201 catgacaacg cattagtagc agcagctact ttatctgacc gttatattac tgatcggttc
     1261 ttaccggaca aagcaatcga tttgatcgat gaagccagtg caacgattcg ggtagaaatg
     1321 aactctatgc caacggaatt agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa
     1381 gaagctgcac tgaaaaaaga atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa
     1441 gaagaattag ctgaacttcg tgaagaagca aatgcgatga agatgcagtg ggaaacagaa
     1501 aaagaagaag tcaattctgt ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa
     1561 ttagaagatg cagaaaataa ctatgattta gaacgcgctg ctgtattgcg tcatggtacg
     1621 atacctcaat tagaaaaaga attgaaggaa ttagaagcta aagcaaaaga cagcgagatt
     1681 aagatggttc aagagtccgt taccgaaaat gagattgctc aagtcgttgg cagattaacg
     1741 ggtatccccg taacgaaact tgtagaaggt gaacgagaaa aactaattaa attgaatgaa
     1801 acacttcata aacgagtgat tggtcaagat gaagcagttg atgcggtcag tgacgcggta
     1861 attcgttcac gggctggatt gcaagaccca aatcgtcctc taggttcttt ccttttccta
     1921 ggaccaactg gtgtcggaaa aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac
     1981 tcagaagatc atatggtacg gatcgatatg agtgaatata tggaaaaaca tgctgtgtct
     2041 cgtctagtcg gagctcctcc aggctatgta ggttacgaag aaggtggtca attaacggaa
     2101 gctgtcagac gaaatcctta tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc
     2161 gatgtcttca atatcttgtt acaagtccta gatgacggac gcttgactga ttcaaaaggg
     2221 cgagtagtag atttcaaaaa tacggttctg atcatgacca gcaatatcgg ttcacagtta
     2281 ctgctggaag gcgtgactgc tgatggaacg attccagaag cagtggctga acaagtcaac
     2341 acgctattac gtggaaactt caaaccagaa ttcttgaacc gaatcgacga cacgatttta
     2401 tttacaccat tgagcctaga caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg
     2461 gcacaacgtc tggaacatca agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg
     2521 attgctgaaa atgcttatga accagcatat ggtgcaagac cattgaaacg atttatcact
     2581 aaggaagtag aaacaccact agctaaagaa atcgttgcag gacatgttat gcctaaatca
     2641 aaagtaacga ttactttact tgatggacaa cttcatttca aaacagaaga gctagaagaa
     2701 atcgtctaat cttccattga gactataaaa gaaaaaaggg gtctgacaca actagtgtca
     2761 gacctccttt tttcaaataa acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa
     2821 atttcttgaa gctgcccgct tctgtcacaa cctcttttat taggtatttt tcactttttc
     2881 taatttataa ctttcaaagt aacgctcatc ttttttactt tgagaccatg caaacaatcc
     2941 cattaaggca gcacctatcg tatttgtcag caagtcgccc atcgtatcca tcaatgctgc
     3001 tcgtccgaca aacagtgtgc catcagaagc agcaaaacgt tgaagattca tgcctaagaa
     3061 ctggtcgcat aagaattccc agaactccca aaacacgccg caaagtccag caaacgcaaa
     3121 gccgaataag aggaacaacc aaggactggt ttttgatatc tcagcatctt tcatcaagta
     3181 accaatcaat ccatagccaa gagctgtcag gaccattgga ctcaccgcat gtaagatttt
     3241 gtcccaaaaa gagatgatac tgatcaaatg catccctgtg cctaaaaaga cagagatgaa
     3301 caagaaaaac cagtaaaaca aaacgattgc tgctggtatt ttaagtttga aaactttcaa
     3361 aatgacttgt ggcatgaaaa tcaatgcaat ccctaataaa gattccacga taaatgtgat
     3421 tccttgagaa gtcgaatatt tttgtgtgat aaattcataa aaattatagc agacaacgaa
     3481 tacaccaaaa gcaagtaaag ctatttgcac ctttttgatt tctttaatca ctgaaagtcc
     3541 ctccatctaa aaatcttttt acttctgaaa gcaactcaat ttctgaagaa accacttttc
     3601 cgttcaattt gatcaaacct attgtatata agttcaggta gtgaaattga ttttcagctg
     3661 tttcttgtag tgcagcgatt tttttcgggt gggttccgcc ttgctgtcga gtatcagtat
     3721 ataagccgat cactgggatg cctttcgcat aagccacacc gatttccgat gctaccccgg
     3781 catcgatcgt cagcccatct agtaaagcaa tcattaaatc actagccaac actttttctg
     3841 tgtcagcaag agcaatcatt cgactgtctg cataagcagt tttgtcattg attgcttcat
     3901 tttcttgagg aagatagatc gaaagctcag aagagagctc tctgatttct ttaactaact
     3961 gttggttata taacaaatca cttttagcaa acattggtgc ggcaaaataa attttcatct
     4021 aaacatcatc ctttttatca aaaataatcg ctaaagtaag cttccatgtt tggaatgata
     4081 cgctctagtg acttcagtgt ttcttcatct gtttctaacc gttcgattct tgccaaataa
     4141 gaagcacggc gataattcat catcaagcaa gtcaatgttt ggatatccag tcgaacggat
     4201 tttcctatag gttcatttaa gacacggact tgtccctgtt catcccatgt taaagcgaag
     4261 acaccattat tccaaggagc gacaggatct tcaatgatga aatgaaaagc atcagctgtt
     4321 ccaacaaacg gaaaccgttg aaggaattct tttacatcaa cgatccgagc cataaaatat
     4381 ggttcgatct gctctttgat ctgactatct tcaatcaaaa atgctaaagg ctcattttta
     4441 taaatatctc catgtaccca gtagatcatg gaaaaatgtg cggaaataaa gttccataag
     4501 ccatttcgag cttcttgatc aagatagaac atttctttta cgtgaaatat ttcttcagct
     4561 acccaataga acagaacacc tcttggttca tggttgctat cgtagtagac agcggctgtg
     4621 cgctcatctt cattttcgaa acgccaatac tcttcccaat gaaaatcact gcggaataaa
     4681 gcaccatggt tttgttgggc aaatttgtca taaacggaaa atacgtctgg atgatcaact
     4741 ggtaatcgct caaccatacc acttactggg atttgtttag gtagctgtgt atctctgatt
     4801 ttaaaggaaa gtttatcaga catgatttcc catcctttac gccgatagta ggggatactg
     4861 taaggataca aataagagat ccattgctta ttcttgcgca tattatccaa tgcaacgata
     4921 attagatctt gcatcaatcc atggttagca tattctggat aagttccaac tccagtcaca
     4981 ccacccattt tataacgtgc accgtgaata ttgacttcac aaggatagat agcgatttga
     5041 gagatcagtt tattttcgtg gaaccatccg aacactttcg atacttccaa aatcggtttt
     5101 ttggaacgga taaaagctcg tttattctca aacccgcttt cctcgatgtc tgcttcagtc
     5161 acttggaaca cataggaaag caattcattg aattgatcga tatgttcttg agtgaccggt
     5221 tttaattcaa gattttctct aaaatctttt tgatccattt tttcatctcc atttattagc
     5281 ttgataacag tatagcaaaa ttctacaaaa aaaacttcta tagctgacta attaaaaact
     5341 ctttaaatta ctgcttttcg ttggtataat ggaaagtgct gattatatga aaagaggaga
     5401 cgctatggat ataaagaaaa tgaaagaacg ccaagagcag attcgtaatt tttcgatcat
     5461 cgctcatatc gaccatggga aatctacatt ggccgatcgt attttagaaa tgacgcacac
     5521 tgtgacgtct cgcgaaatgc aagatcagct gttagactcg atggatttgg aacgtgaacg
     5581 tggaatcacg attaaattaa atgctgtaga actcaattac acagcgaaaa atggagaaac
     5641 atatatcttt cacttgattg acaccccagg gcacgtcgac ttcacctatg aagtctctcg
     5701 aagccttgct gcctgtgagg gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc
     5761 acaaacattg gccaatgttt atttagcgtt agacaatgat ttggagatcc tgccagtcat
     5821 caataaaatc gacctacccg cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt
     5881 aatcggaatc gatgctagtg aagctgtatt agccagtgcc aaatcaggga ttgggattga
     5941 agatattctc gagcagatcg tggaatatgt tcctgcacca agtggagact tagatgcacc
     6001 gctaaaagct ttgatctttg actctattta tgatagttac cgcggtgttg ttctaaatgt
     6061 ccggatcacg gacggtatgg tcaaacccgg agacaaaatc aaactgatga gtaatggcaa
     6121 aacatttgat gttacagagg tcggggtctt ctcgccaaaa gcagttgcta gagacttttt
     6181 gatggtcggt gatgtcggtt atatcacagc aagtatcaaa actgtccaag atacacgagt
     6241 aggggataca gtaacgttag cagacaatcc tgccgctgaa gctttaccag gttaccgaaa
     6301 aatgaatccg atggtttatt gtggtcttta tccaattgat acttctcgtt acaacgattt
     6361 gcgggaagct ttagaaaaac tgcaattgaa tgatgctgct ttacaattcg aaccagaaac
     6421 ctctcaagca ttaggttttg ggttccgctg tggattcctc ggacttcttc atatggatgt
     6481 cgtccaagaa cgcttggaac gggaattcaa tctagaattg atcacgactg caccatccgt
     6541 tatttaccat gttaataaaa cggatggtac gactgctaca gtagataacc cggcagattt
     6601 tcctgaacct gtaacgattc aggatgtaga agaaccattc gtcaaagcac agatcatggt
     6661 cccaaatgat tttgtcggtg cagtaatgga actttctcaa cgaaaacggg gagaattcat
     6721 tacgatggat tatttagatg attaccgagt aaatgtcgtc tataatattc cgctttctga
     6781 aattgtcttt gactttttcg acaaattgaa atcaagcacg aaaggctatg cctcattgga
     6841 ttatgaaatg tctggttatc aaaaaagcaa gttagtgaag atggacatct tgctgaatgg
     6901 agaaaaagtc gatgcattaa gctttatcgt tcaccgagac tttgcctatg aacgtggaaa
     6961 agcaatcgtt gaaaaactga aaaaactaat cccacgccaa caatttgagg tgccaatcca
     7021 agcagctatt ggacaaaaaa tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt
     7081 cttggctaaa tgttatggtg gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa
     7141 agaagggaag aagcggatga aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat
     7201 ggccgttctg aaaatggacg aggacgaacc gaagaaatag caagggtttg tttgaaaaca
     7261 gtgtaaaaaa taaaccatgt ttacatggat ttgccaccgg tttgccaccg gtggtttttt
     7321 tcggtggcaa aaatcaagaa ttctttctaa gattttccat gatatcgacc gtttcatttc
     7381 tcatcttatc tgttacatgc gagtaagtat ccatcgtaat tgaaattctg ctgtggccca
     7441 atcgttcaga gatttctttc atttttgcgc cattttcgag aagaagtgta gcatgagtat
     7501 gtctgagaga atggaagtta aaagagaggg agagtgcatt cgatatcctc ctagtattcc
     7561 atttcactac acttggcgta actagctcac cgtcttcctt cgtacatact gcatttgaat
     7621 caatgtagag ctttccatac ttcattcgat tttctaattg ttgtttctta tgttttttca
     7681 gaattgctag caaggtttgt ccaataaaaa tcgttcgatt ggaactgctt gtctttggcg
     7741 taccatatac ccatgcgccg tcattcttta ccatttgttt ctctacagta attgttccat
     7801 ttgaaaaatc gacatcatcc cacgtcagac cacaaacttc gccaacgcgc attcccgtat
     7861 aaaatccaat attcaaagga atatagaaag gatgtccttc aggagtaatt tctagcatat
     7921 gatcaaagtc ctcaagagaa atgattttta gatctttttt agtcgttggt cgtgcttcat
     7981 attttggtat ttttacatac agcataggat tttgcttgat taatccccaa ggataaaccg
     8041 ccatattcag cgcattctta aggacagagt gagtaatagt cattgttttc ttcgagtaac
     8101 cctttttaaa ttcagcattg atgaaatttt gtaaaagagc aggggagaga tccgtaagtt
     8161 ttttctttcc taaataaccg tttatatgat ttttgatggt aaatcggtag ttttcatagg
     8221 tattgtattt tagatttagt ttaacgtatt cctccatcca aaaatcaagg tattgtttta
     8281 ctcgagtatc cgtacctaaa aagtattgtc ctgtttcgtc aatatctgat aaaacttttc
     8341 gtaaagcagc ttcggcctct ggtcgggtgt ctccgccaac tttctccact ttttttcttg
     8401 agccatcatc attgatatct
//
LOCUS       DACDKL010000109         8351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDKL010000109 DACDKL010000000
VERSION     DACDKL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.109"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..321
                     /locus_tag="IV329_001907"
     CDS             <1..321
                     /locus_tag="IV329_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8941077.1"
                     /translation="TSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLV
                     SLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLT
                     RKED"
     gene            414..1576
                     /locus_tag="IV329_001908"
     CDS             join(414..690,690..1576)
                     /locus_tag="IV329_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8941078.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            1856..2836
                     /locus_tag="IV329_001909"
     CDS             1856..2836
                     /locus_tag="IV329_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8941079.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            2958..4376
                     /locus_tag="IV329_001910"
     CDS             2958..4376
                     /locus_tag="IV329_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8941080.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4447..5916
                     /locus_tag="IV329_001911"
     CDS             4447..5916
                     /locus_tag="IV329_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8941081.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            5926..6393
                     /locus_tag="IV329_001912"
     CDS             5926..6393
                     /locus_tag="IV329_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8941082.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6429..7301
                     /locus_tag="IV329_001913"
     CDS             6429..7301
                     /locus_tag="IV329_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8941083.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7382..>8351
                     /locus_tag="IV329_001914"
     CDS             7382..>8351
                     /locus_tag="IV329_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8941084.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIY"
BASE COUNT         2867 a         1262 c         1704 g         2518 t
ORIGIN      
        1 actagtatta cggaattagc tgatcgatta ggcgttacca aaggagctgt atcccaacag
       61 attcccactt tagagaaaat gaatcttata agcatttctc aaaaaaaagg aaataagaaa
      121 actaaacttg tatcgttaac aaaatatggt cgaaaggtgt acgaatcaca taattattta
      181 catgaagagc tttatagctc aataaatgaa gcgttagaat cattctcacc agaccaaatt
      241 tcaaccatta aacagattct agaaaaagta tcttatacaa tcaatgaata tcaagtaagt
      301 ctgacaagaa aggaagattg acatgaaaaa aatgattttt gaatcggcta cactagacta
      361 gacagaaaaa ataaggtgtg taaaataaat gaaaacacac tggaggaatt tttatgtcaa
      421 gacgtcaacg tagaacctat tcaaaagaat tcaaacaaca agtcgtcgat ctttatctcg
      481 caggaaagcc ccgagctgaa atcattcgag attatgagct gactacctcg gcctttgata
      541 agtgggtcaa gcaagcgcaa actactggct cgtttcagga aaaagataac ttgatccctg
      601 aacaagtaga actaattgca ttacgaaaga aaaataaaca gcttgaaatg gagaatgaca
      661 ttttaaagca agcggcgctg atattgggac gaaaagacaa gtaattgata ccaataagca
      721 caaatactca atatcagcga tgtgtaaagt gttaaacatc tcttgccaaa cctattatta
      781 tcaagccaaa ccagttgaag atgaatcaga actggaagaa ctcgttcaag aagaatttat
      841 tcgcaatcga aaagcttatg gtaccaggaa gctgaagcag tgcttggcca atctaggtct
      901 tcaagtgagt caacgcagaa tcggccgtat gatgaaacag cgtggcttgc gttcaacata
      961 cacattggct catttcaaat tccattcttc tacttgtaac gaagccaaga ccgccaatat
     1021 tctagacgga accttcctac aggaaaagtc attagaagcg attgtaaccg accttaccta
     1081 tgttcgtgtt gggaagaaat ggcagtatat ctgcctgatt cttgatttgt ttaatcgaga
     1141 aatcatcggc tattcatgcg gggagaagaa agatgcgacg ttagtcaagg aagcttttgc
     1201 aaagattccg tactcgttga ataatgtaca actttttcac acggatcgtg gaaaagaatt
     1261 cgataaccag acgattgacg aaattttatg ggcctttaat atcacgcgtt ccttaagcaa
     1321 aaaaggctgc ccctatgata acgccgttgc agaatccacc tataaatctg tcaaagtaga
     1381 attcgttcat caataccgct ttgagacatt ggctaatttg cgattagagc tatttgatta
     1441 cgtccattgg tggaatacca tccggctgca cggcacgtta ggctatgaga caccgatcag
     1501 ttttcgacag cagagattgg cgcagcgaat ccttgataat gagctcggat gtgataccgg
     1561 tggcgaggca ccttaattat ttgaatgagc ttctgcctta gagaatcaga ttcgaggagg
     1621 ctcgttgtca agggcagtcg gaacaccagc agcaagctta cgcaatacct tataattttt
     1681 gtcaaaaaaa gtgttgccat tccacatata tctttaaaca gctattgcca gataaaaata
     1741 aatgatatta tatatataaa tacttatccg aaaaggggaa tgagagagtc aaggttcttg
     1801 gaatgtcgaa tagtcgagta ggtagatttt ctatctacaa ccttgttgtt taaaaatgaa
     1861 aaaaattgga attagagaag ttgcgaaaga ggcaaatgtt tctcctacga ctgtgtcgag
     1921 ggtattgaat gatcgtggat atttgagtga ggaaacaaaa agaaaagttt ttgatgccat
     1981 gaaaaaatta gaatactatc ctaatgaact ggcgagagca ctttttaaaa acgaaacata
     2041 taccgtaggt ttgatttttc ctacaattac taatccattt cacgctgagt taattcaaga
     2101 tatcgagttc gagctttcca gcaggggata taaagtgttg ctgtgcaata gcttaaacaa
     2161 tccaacaaaa gaaaaagagt acttgacgat gttgagaaag aatcaggttg atggaattat
     2221 agtaggaacg cacaataaag atattgcgga ttatgatatt ccagggctac ctattgtagc
     2281 gattgataga agtcttggtg aaaatacagt tacagtatct tgtgataatt acaggggtgg
     2341 ggaattagct actcaactac ttgtaaatca tggctgtcag aatatactgt gtattcgggg
     2401 agatagtaag ataaagctac ccgcgaatag taggacttta gcatataaag atgttttgaa
     2461 taaattcatg ctggaaccgt tagtcctaga agtaccattt gtaaaagaaa tcaatgaaaa
     2521 aaatgaaata attaagacat atctggaaac tcatccaaat attgacgggg tttttgcagg
     2581 agatgattta ttggcttctt tggcgattaa caacttagga agttcgggca aaagagttcc
     2641 aaaagaggtt aaagtgattg gatttgatgg agctcaacaa acattgattt ataatccaga
     2701 attatcaact attcaacagc ctatagatca aatatctaaa gtagcagtaa aaaaattatt
     2761 gaatatgatt aagggcgaaa aggagacgga tgaactttca cttccagtaa agctcataaa
     2821 aaaaaataca gcataagtta aatcggcgaa aaattccttc gtcgatttaa aaatattttt
     2881 atgaaacggg ttgacatatg aaacgggttg acatatatta gttgtgaaga taaaaacctt
     2941 ttcactggag gaagaaaatg gagaaattat taattaaaag caataatcat caactttact
     3001 accaacctaa ggatgtttgg gtcggagatg ttatgcctta cgggaaagaa ggaaaattct
     3061 ttatttatca tcaacgtgat actagaaatc cagttccttt tggagaaccg tttggctggg
     3121 ctttagcaac tacgaaagat tttgttcatt ttgaagactt tggggaatct cttttaagag
     3181 gggatgacaa tgagagagac cagtttatct acgctggttc tgtatttgag gcaaatggaa
     3241 aggttcacgc attttataca gggtataatc gtgaattctt agcgcaggga aagacatctc
     3301 aagtgttgct tcatgctgag agtgatgatt ttgttcattg ggaaaaatca gaaacagcat
     3361 tggagttaga accacaagaa gggtatgata ttcgaaactg gagagatcca tttgtcgttt
     3421 gggatgatga aaaagaagaa tatttgctaa ttttaggggc gcgaaagggt caggataaac
     3481 atctacaaac cggacgttta gtggaattta catcaaaaga tttaactaat tgggaattca
     3541 aaggtgattt ttgggctccg ggattgtata ccatgtttga aatgccagaa cttttcaaga
     3601 ttggtgactg gtggtatctt gtctattcag aatactcaga tggaaataaa atactctatc
     3661 gaatgagtca aagtttggaa ggtccttgga ttaagcctaa agatgaagct tttgatggac
     3721 gggcttatta tgcaggtaga acagcttatg atggaaaacg tcgagtccta tttggatggg
     3781 taccaacaaa agatcaagaa aatgataaaa acaattattt atggggaggt actttggttc
     3841 ctcatgaagt ctatcaaaaa gaggattttt cacttggggt aaaacccata gacgaagtgt
     3901 ggaatgcatt cataaattta catgatattg ccgatgtaga gatagaaaca gcagacacaa
     3961 gagaagaagt aacgcttgtc aaaaatagtg gaactttgtt taatttagaa acaacaattt
     4021 catttgatga gaatcttagt gacttttcac tgagattata taaaaacctt gagacagatg
     4081 aatcttacga atatcgcttc aatttagatg aggggcgtct tacatttgat aaaaaccctt
     4141 gttacccttg gtttcaatat atggataaag gacttattag acctatatct ttaaagccta
     4201 accaatctta taaactaaaa gtgattgttg atgacaatat aatgacactt tatattgatg
     4261 gaatcgctct taacgctcgt gtacatggtt gttttggtca agcaattact attacagcaa
     4321 caaatggatc aatgactgca ataaatacca agttttcaaa tgaaataaag aaataataaa
     4381 cgtcaatcat gtggcaggtc tgaaaatcaa acatgacctg tcacatttaa aagggaggat
     4441 aaatgaatgg actacaaaaa aattgccgaa gatgtattgg aaaacgttgg aggaaaagag
     4501 aatatctcaa gcgctacaca ctgcattact cgtttacgtt taattcttaa agacaaaagc
     4561 aaagcgaata tggaaatgct tgaggaaata gaaggtgcaa aaggtgttct tttcaatagt
     4621 ggacagttac aagtaatttt cggaacaggt ataattgaaa aggtttacaa agagttcgtt
     4681 gaattgacag gagctaagga agtatccgtt tctgaaatga aagacgaagg agtcaataat
     4741 tcaggaaata gacttcaacg agcttttaaa gttttctcgg atatctttat tccgatcatt
     4801 ccagctttta ttggggcagc aatggtttta ggaattcgtt cgttgattac cactcctgga
     4861 atgtttggaa tggatggctc attcgctgat cacaatgtat tcctaaatga tttatccaaa
     4921 tttctgggta ttattgcaac cacatttgat tatttaccag ttttagttat gtattctgca
     4981 acaaaacgtt ttggcggaaa ccctatttta ggattacttg taggatttgt tatgattcat
     5041 ccaggactag ccaatcggaa tgatttcgtc atgggaacta taaagcctga ttattggcat
     5101 ttatttggat taagcattcc agcagttgcg tttcaagggg gtgtttttcc agcaattttg
     5161 acttcttggt ttttagcaaa agttgagaaa attacaaata aatattctcc tcaagtactt
     5221 gcttacattt tagtcccaac tgtgacaatt gttcttgcca actttgcact atttttagtg
     5281 tttggtccat tggggaattt tatcggtaat gttttaggtg gaatcattga tttcctatat
     5341 atgaagatgg gtgcctttgg tgcttttgtt tttgcaggcg cgttacaacc attagttgta
     5401 acaggaacac aacatgctat tcaaggtatt gaggcaaact tagtcgcaac aactggattc
     5461 aattatatcc aacctatttg gtcagtttca attattgccc aaggtggcgg ttgtattggt
     5521 atgtatttac tctttaagaa aaaatctaaa gatagagata ttgcgatttc aagctttatt
     5581 ccaacactgg taggtattac tgaaccggct atctttgctg ttaatttgaa atattcaatc
     5641 gttccttttg tttgctcgtt tattggtgct ggttttggtg gagcatttat gaaaattttt
     5701 gatgtaaaag ccattggaca aggattgact gtattgccag gtctgactat tgctaatcca
     5761 gttcttcctt atatatttgg taatctcata gcattcattt taccaatcat ttttattctc
     5821 gcttatgata aatataaagt gtttatccct gaagctaaac accgtaaatt tgcagaaaaa
     5881 actgactcaa aaaaattgcc agttcaagta aagtgagatg agagcatgtt aaattttttt
     5941 agaaaaaatg aaataagcgt tgtagcacct gcaaccggtg tttttgaaaa actggagaaa
     6001 ttaagtgatc cagtcttttc aaaaggaatg atgggacaag gatttgcaat tgatgcaaaa
     6061 aacgaaacga ttgtatctcc aatcgatggt tttgtttcaa gtatttttcc tacaaaacat
     6121 gccttaggaa taaaaacaaa atcaggaatt gagatacttg ttcatgtagg aatagataca
     6181 gttgagctaa atggtgaggg atttgatatt aaagttcaag aaggacaaaa agttaaggct
     6241 ggggatattc ttatgaccat taattttgta gtttttgaaa agaatcacaa gactaaagat
     6301 gtcattgttg tttttccaga ctaccaagac gaaattttca ttccgaaact tgaagaagta
     6361 aaacaaggac aagaattgtt tgttcttggc taatgataag taattaataa tagaatcgag
     6421 gaaaatgtat ggtttttggg gctattgaag ctggtggaac aaaatttgtt tgtgccattg
     6481 gagataaaga tttgaacata tttgaaaaag tcagctttcc cacgactaca cccaaagaaa
     6541 caatgaattt agttttagat ttttttaatc atcataaaga tgatttagaa ggtatcggag
     6601 tgggatcgtt cggtcctatt gatattcatt gtgaatctaa aacgtatggc ttcattacat
     6661 caacgccaaa acttgcttgg caggattttg attttattgg aagtttaaaa aagcaactta
     6721 atattccaac tgcttggacg actgatgtaa atgcctcttg ctatggtgaa tatgtttctg
     6781 gaaatggcag aggcctttca agtgttgttt actataccgt aggaacgggc ataggaggtg
     6841 gcgctatcca aaacggaaac ttcattgaag gttttagtca tccagaaatg ggacatatgc
     6901 ttgtaaaacg acacaataaa gacaactttt ctgggacatg tccattccac ggagactgtt
     6961 tagagggctt agctgctggt ccggcaatcg aaagaagatc agggaaaaag ggacaagaag
     7021 tgactgaaaa tgacgttact tgggaaatag aagcttatta cattgctcag tgtgcatata
     7081 atacaacatt gatgttatca cctgatgtca ttatatttgg tggcggagta atgaaacaaa
     7141 aacacttaaa aaagaaagta caagataagt ttattgagtt gatcaacgga tatgtcgcta
     7201 ttcccgatgt tgacaattat attatgacac caaaattgtt agataattct ggtattgtag
     7261 gttgtttagc tttagcaaga gacgttaaag aaaaatctta atttggaaaa aagatcgtct
     7321 cgactatttg tttggttgat gcgatctttt tttcaattag taattgaagg gacgcctaaa
     7381 agtgaaaaaa aaaatgaaag atgaagtatt agagtatatc aaaaataatt taaagtatta
     7441 cgatttttct gctcaagata ttgcaatgaa attttgtatt aaaagaaatg tcgcttccca
     7501 ctatttgaat cagttatttt cagatggaaa attactaaag aatgattctg ttagaccagt
     7561 aatgttcaag tataatcaac aaaagccaaa agactgcttt tctaagttta ttggagcaga
     7621 catatctttg aaatccacta tagataaatg caaagctact gtaatgtatc cccctaatgg
     7681 cctcccatta ataataaaag gaaacagtgg cgttggaaaa agttttttag caagtttgat
     7741 ctatcagtat gctcttgata gaaaagtaat tcataatgac gcaaaatttg ttgttgtgaa
     7801 ttgtgcagat tatgcaaata atccagagct attgtcagca gttttatttg gatataaaaa
     7861 aggagccttt actggggctg aaaaagatac tgcagggatg ctatcttctg ctaatggagg
     7921 ataccttttt ttagatgagg ttcacaatct aagtgcagaa aaccaagaga aattgtttct
     7981 tcttatggat tctcaaaagt atcgtaaact tggtgaaagt gtgcaatggg agtatgcaaa
     8041 tgttcgcctt atcttagcca caacagaaga taaaaatacc tctttgttag caacttttag
     8101 aagacgcatc ccagctgaaa tcactctgcc agattacaat aatagaagta catctgaaaa
     8161 aattcaatta ttatttgaat ttcttaaaga tgaagcaatt acgattgatt caaaaatatt
     8221 ttgttcgatt gaacttttta ctgatctttt atcgaaaaca tttgaaggaa atgttggaga
     8281 attgaggaat gaaatcaagc ttctttgtgc agaaggttat ttaggaaata agagaaagtc
     8341 ttcaatttat t
//
LOCUS       DACDKL010000110         8205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDKL010000110 DACDKL010000000
VERSION     DACDKL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.110"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..563
                     /gene="lepB"
                     /locus_tag="IV329_001915"
     CDS             12..563
                     /gene="lepB"
                     /locus_tag="IV329_001915"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8941085.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(646..1188)
                     /locus_tag="IV329_001916"
     CDS             complement(646..1188)
                     /locus_tag="IV329_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8941086.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1425..1823)
                     /locus_tag="IV329_001917"
     CDS             complement(1425..1823)
                     /locus_tag="IV329_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8941087.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2083..2943
                     /gene="ylqF"
                     /locus_tag="IV329_001918"
     CDS             2083..2943
                     /gene="ylqF"
                     /locus_tag="IV329_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8941088.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            2936..3703
                     /locus_tag="IV329_001919"
     CDS             2936..3703
                     /locus_tag="IV329_001919"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8941089.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3759..4616
                     /gene="dprA"
                     /locus_tag="IV329_001920"
     CDS             3759..4616
                     /gene="dprA"
                     /locus_tag="IV329_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8941090.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4738..6816
                     /gene="topA"
                     /locus_tag="IV329_001921"
     CDS             4738..6816
                     /gene="topA"
                     /locus_tag="IV329_001921"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8941091.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6785..8161
                     /gene="trmFO"
                     /locus_tag="IV329_001922"
     CDS             6785..8161
                     /gene="trmFO"
                     /locus_tag="IV329_001922"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8941092.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2861 a         1445 c         1724 g         2175 t
ORIGIN      
        1 attgatagag attgacaaaa aaacaacgct atattaatcg cttctggctt gtattcaaat
       61 acttacttgt ttctgtattc cttgcgttca tgttgcgggg atttttattc atcccggtac
      121 cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt gtgatggaaa
      181 aattttcgga gatcagacga tttgatattg ttgtttttca attagcagat ggcacgattt
      241 atatcaaacg agtaatcggt cttccaggag aaaatgtcag ctaccaaaat gatcaattga
      301 agatcaatgg gaaagtagtt aaggaacctt atctaactaa aaacctgaaa tcagatcatg
      361 caaatgcatc ctatacgacg gactttacac tacaagagct aacgggacaa tccaagcttc
      421 cggaagataa ttattttgta cttggagata atcgaagagt ttcaaaagat agtcgttcgt
      481 ttggcacgat caataaaact gatattctag gtaaagcaag atttgtctat tatccacttg
      541 atgaaataaa atggatccag tgagaaacaa taaagcgtgc agtctgttga gactgcacgc
      601 tttattgttt aatccaaaat cagtttacta ccttagtacg taaaatcaaa cagctcgttt
      661 aaacactttt ctagcctcta aagttttgat aaatgaataa acaaccatta tttgaatagg
      721 gatttggatc atattcctga tgaatctacc tagaaaaatt actttgaaat ctgcaccaaa
      781 caaaacagac atcagccaag aattgataaa aaggctgact gggatcataa tcaataattg
      841 ggcagcgatg acggaaggca ggtttatctt tttatgatat aaaaataagc cattaataaa
      901 tccaccaagg aaagccgtga taagaaaagc agcttgaaac gttcctgaac caaatatcgt
      961 gaaatttaaa gtgttcgaca acatttcaag caaaccagcc cagatcggtc catatagata
     1021 acccataagc gccatactta aaaatccgaa tgtgatttga aaataagcag gaccaatcgc
     1081 tatcttgtat aagatgacat caagggctaa taaaagagca gaggtcgaca acaatcttgc
     1141 attcatttcg ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa tattctctcc
     1201 tttgtcagtt aataataaaa ggataccata aaatcaccaa tgtgtcatga cacattggtg
     1261 atttacatac actggcatct aacaattaaa aaaagggtga gaagactgct tttataaact
     1321 gtaaaaaaga gattgtacga ataaaaaaaa tagaggcgga gaatacttat cgtatcatcc
     1381 gcctttgttt ttttgttata aaaaacgtca gattagttat ctgcttacat cttcatccat
     1441 acgggttcta atgtatttcc gtctagatct tggacttcta atgaaaacat ttcttcttca
     1501 ggcattccca tatctacttc gtggaaagta ccaccatttt cttttgcaat ttgagcaaat
     1561 ttttttactt catccgcgtt ttcaacacta aacgaaatca aagcaccact tgttttttta
     1621 ttatcagcta tttctttatc ttttataaat tttttataaa aatcatgaga gagaagcatt
     1681 atccaaaaag tgtcatccca taccattgaa ccagcttctt cagtagaaaa ttcttcattt
     1741 tttttaaaac ccagcttttc ataaaaagca acggaacgat tgacatcagc aacaggaaaa
     1801 ttaacaaaaa ccatactatt catttgtttc gtctcctttc tgttttgttc gtctatcatc
     1861 tcttatttag gaaacgctgt caaaaattta gattcatata tagaaaccaa aataaaaatt
     1921 tgcatcatca aaaaagtgaa gatgtgatag aatgaggact ggttcacttt cacttaacta
     1981 atagatggaa tttctttata ataaaaaagt gaatttatat tgaaaaagaa aaaaacaagt
     2041 aaagccgggg aatattccgg tatgaaagga atggagagaa caatgaccat ccaatggttt
     2101 ccgggacata tggcaaaagc ccgacgagaa gtttctgaaa aaatgaaata tgtggacatc
     2161 gtatttgaat tggtagacgc acgattacct ttatcttctc gtaatccaat gctagatcag
     2221 atcattcagc aaaaacctcg ccttgtttta ttgaataaag cggatctggc agatccacaa
     2281 cagacaaagc tgtggcaaga atattttcgt aaacaaggcc atcttgcatt agctatcacg
     2341 gcacaagaaa gtaaaggaat taaaggtcta gtacctgcgg ctaaagaagc attgaaagaa
     2401 aaacgggcaa gagatgctgc tcgggggatc aagcctcgcg ctattcgggc aatggtctta
     2461 ggtattccaa atgttgggaa atctacgttg atgaatcgac tagtaggcaa gaaaattgca
     2521 cagacaggaa ataagcctgg cgtaacaaaa ggtcagcagt ggcttcgttt aggtaatgag
     2581 ttggaattgt tagacacacc agggatttta tggcccaaat ttgaagatga agaaatcggc
     2641 aaaaaactag cgctgacagg cgcgatcaaa gaccagcttg tccatttaga tgatttagcg
     2701 atttacggat tagacttttt tgcccgatat tattcaggaa gaatcagcga tcgttatggt
     2761 ttatcaatcg agcaagaaga aaaattggca ccggaattac tgatggatat tacagcaaaa
     2821 cgtgggtata aagaagacta tgatcgtggc agcgaattag tcgtgcatga tattcgtcaa
     2881 ggaaaacttg gacgatacac actggatcgt tgcgaagaaa tggagcaaaa aagtgatgac
     2941 tgaatccatc caaagtatca aagaacgatt gaaaagcata cagagaatag atgatcctac
     3001 actagaatca ttaagaaatg atccacgcaa aggcgtgcag caagcactgg cttcttttga
     3061 acgacgattg aaaaaggaag cattaattaa ggaaaaatat gaagaaatgt tttgctttga
     3121 aaagcaaggg tacgcacaag gtcaccaatg gattgccggc attgatgaag taggaagagg
     3181 accattagct ggacctgtcg tggcagctgc agttatcttg ccgaaaaatg agatcatatt
     3241 agggctgaat gactccaaac aattatcaga gaaaaagcga gaacttcttt atcaacaagt
     3301 tcaagaaaaa gccctggcaa tcgggatcgg gatcgttgat cagcagacaa tcgatgatgt
     3361 gaatatttat gaagcaagta aattagcgat gatccaagca gtagaaaaat tgtccattgt
     3421 accaagctat ttgttgatcg atgcgatgac cttggatttg cctattcagc aggaaaaaat
     3481 aatcaaaggg gatgcacgat ctgtgtcgat tgctgctgca agtatcattg ctaaagtata
     3541 tcgagaccaa ttgatgaaag aatatgacgt gctttatcct ggatatggtt tctcgcataa
     3601 tgcaggttat ggaacaaagg aacacttaga aggacttgaa cgacaaggaa ttacgccaat
     3661 ccatcgtaaa agttttgcac ctgtaaaaaa atatttacaa taaaaaacag ccgcttttac
     3721 gtatcttatt agaaagagag acgaaaggcg gttttatgat gtatcaaata gaagaaaatt
     3781 tattgaaact tgcttataca aaaggaatca gtagtcaagg aaaatgggca cttgctaact
     3841 ggatgatgca gtatccttat aaagaaatcg gatttgaaga catcattaag ataggtaaaa
     3901 taaccaaaca tcgggagctg tttcgcaaaa gttggcagga aattcacgct aactggggca
     3961 aaatccaatc gaaacagtcg tttatcactt gctttgatcc caaatatccc cctcagctgc
     4021 ttcacttgac ctatccgcca attgtactgt tttataatgg tgatctatct ttactttctt
     4081 gtaacatgtt aagcgttgtt ggtggaagac acacttctgc tttagccaaa aaaacagttt
     4141 attatttact aaaaccagtg atagaagctg gatatgtgat cgtcagtggc tgcgcgaaag
     4201 ggatagatac atacggtcac caagcagcaa tctctcatgg tggacggaca atcgcagtca
     4261 ttggaacagg gatagagcaa gtctatcctg ctgaaaatcg tgacttgcaa gaggtgattg
     4321 caaatgatca tcttcttttg agtgaatatc ctcctcaatc cggcccgaaa aagcattgtt
     4381 ttccaatgag aaatcggatt atcgcagctt tgtctaaagg aacatgtgtc tttgaagcca
     4441 agaaaaaaag cggctctctg ataacagcac agcaagcatt ggatcttgga agaacagttt
     4501 tttctgttcc aggaaatatt ttaacagaac attctaccgg atgtcatcat ttgatccaag
     4561 acggtgcagt ttgcacggtt tcgggtgaag atatcttggc agaatttaaa gaataacacc
     4621 atacttttca tttgcttttc ttgacaaacg ttttctgaat ggatatgatt agctacgatt
     4681 tgtatgaatc ctcttttaat agtaggaaca cgacaatgga aaggagtcca ttcagtaatg
     4741 gcgtataaat atttagtgat tgtagaatca cctgcaaaag ccaagacaat tgagaaatat
     4801 cttggacgaa actataaagt aatggccagt gttggccata taagagattt accgaaaagt
     4861 aaaatgggta tcgattttga aaacaattat gaaccccatt atatttctat acgcggaaaa
     4921 ggcgatgtca tcaaaagcct gaaagccgca gcaaaaaaag ctcaaaaagt ttacttggca
     4981 agtgacccgg atagagaagg agaagcgatt gcttggcatt tagcgtacct acttgggttg
     5041 gatctgaaag aaaaaaatcg ggtggtcttc aatgaaatca caaaagacgc agtcaaagca
     5101 gcttttaagg aaccaagaac gatcgatgta gatttagtag atgcacagca agctcgtcgt
     5161 accttagaca gaatcgttgg ttattcgatc agtcctattc tctggcgtaa ggtcaagaaa
     5221 gggttaagtg caggacgtgt ccaatctgtc gctttaaaaa ttattattga ccgtgaaaaa
     5281 gagatccgag aatttgttcc agaagaatat tggagcatcg acggtaattt taaaaaagct
     5341 cgcaagaaat tcaaagcaaa tttctgggga atcgacggta agaaaaagaa attaccagat
     5401 gcacaaagtg taaaagaagt cactgctaga attgatggaa cagacttcga agttaaaaaa
     5461 gtagaaaaga aagagcgcaa aagaaaccct gcgaatcctt tcactacaag tagcctgcaa
     5521 caagaagctg cccgtaagtt aaactttcga acaagaaaaa ccatgatggt agcacagcaa
     5581 ttatatgaag gaatcacttt gggtaaacaa ggaacagtcg gcttgattac atacatgcgt
     5641 actgattcta cccggattgc cgatacagca aaagcagagg ccgcagcatt tattgaagat
     5701 acgtatggaa aagaattttc tagacatgat caccgaaaga tcaaaaatgc tcaaggcgca
     5761 caagatgccc atgaagctgt gcgtccaacg agtgtgcttc gtacaccaga tgaactcaaa
     5821 caatatttag ataaagatca attgaaactt tatacattga tctggtcacg ttttgtggct
     5881 agtcagatga caccagctat tttagataca atgaaagtga cattagaaca aaatggtgtc
     5941 atcttcattg cgaacggttc aaaaatcaaa ttcaaaggat tcatgcaagt gtatgtggaa
     6001 ggtagagatg acggcaaaga agagaaagaa aatattcttc cagaacttga agaaggcgat
     6061 gtagtccaat ctgttgatat tgaaccaaaa caacatttta ctcaaccacc agcacgtttt
     6121 agtgaggcta cgttgattcg tgcactagaa gaaaacgggg tagggcgacc ttccacttat
     6181 gcaccaacac ttgatacgat ccaaagacgc tactatgtaa aattgacaca aaaaagattt
     6241 gagccgactg aactaggaga aattgtcaat tcattaatct gtgaattctt ccctcaaatc
     6301 gtcgatatcc atttcacagc agaaatggaa ggagatttgg ataaaattga agaaggcacg
     6361 gaagcgtggg tcaaagtggt tgatcgtttc tataaaccgt ttgaaaaaga actgacgaat
     6421 gcggaagaaa aaatcgaaaa aattcaaatc aaagatgaac cagcaggatt cgattgtgat
     6481 gtctgtggac atccgatggt gataaaatta gggaaatatg ggaagtttta tgcttgcagt
     6541 aattttcctg attgtcggaa tacgaaacca atcgtaaaag aaatcggtgt gacttgccca
     6601 gtatgtcatg aaggacaagt cattgaacga aaatctaaga aaaacagact attctatggt
     6661 tgttcccgtt tcccagaatg tgaattcact tcttgggata aaccagtggg cagaaattgt
     6721 ccaaaatgtg acggatatct ggtcgagaag aaagtcaaag gcggtaaaca agtagtctgt
     6781 gtaaatggag attacgaaga agacatccaa aaataataga aaagaggttc tttcattgaa
     6841 aatggtaaat gttgttggag cagggcttgc cggaagtgaa gctgcttggc agatcgcaca
     6901 agcaggagtc cctgtccgtt tatatgaaat gcgtccagtc aaatcgactg aggcgcatca
     6961 cacaagtaat tttgctgaac tagtatgttc caattcatta agaggaaaca gtttggctaa
     7021 tgcagtaggg ttattgaaag aagaaatgcg ccgattgaat tcggtagtaa tcacttcggc
     7081 agatgaaaca gcagtgccag ctggtggtgc cttagctgtt gaccgcgatt ctttttctga
     7141 aacaattacc cgaaaagtca aagagcatcc attagtaaca gtgataaacg aagaaattac
     7201 tgaaataccg gaaggaatca ccgtagtagc aacaggacct ttgacttctc atccattagc
     7261 agaaagcatc aaagcattca atggttcaga tggtttttat ttctatgatg cagctgcacc
     7321 aatcgtagac aaagctacga tcgatatgga taaagtatat ttgaagtccc gctatgacaa
     7381 aggagaagca gcttacttga actgtccgat gacagaagag gaattcaaag ctttccatga
     7441 ggcactaatc aatgccgaag tggcagaact taaatctttt gagaaagaaa aatattttga
     7501 aggatgtatg ccaattgaag taatggcaca gcgaggattc aaaacgatgc ttttcggacc
     7561 tatgaaacca gttggattgg aagatcctaa aactggcaaa cgtccttatg ctgttatcca
     7621 gctgcgtcaa gacaatgctg cagcttcttt atacaacatc gtgggctttc agactcactt
     7681 gaaatggggc gagcaaaaac gtgttttccg gatgattcct gggttagaaa atgctgaatt
     7741 cgttcgctat ggtgtgatgc accgaaacag ctttatgaat tcgccagaat tgctgaaacc
     7801 gacttaccaa tcaacgaaac gcgaagatct gttctttgca ggtcagatga ctggtgtaga
     7861 aggctatgtt gaaagtgcag cgagcggact acttgctgga atcaacgccg cacgcttagc
     7921 aaaagaaatg gaaccagtcg agtttccaca agaaacagct attggaagta tggcttacta
     7981 cattactcat gcggaaggaa aacatttcca accgatgaat gccaactatg gtctgtttcc
     8041 tgaactgcca gagcgcattc gagacaaaaa agcaagatat gaagcaattg cacaacgagc
     8101 tttaaaagct aatgaggcaa taaaaacaga attagaaaaa actgcaatcc caaatgagta
     8161 aggaaactcc atgaagaggg tgagacggat agtctcgctc ttttt
//
LOCUS       DACDKL010000111         8135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDKL010000111 DACDKL010000000
VERSION     DACDKL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.111"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            42..1160
                     /locus_tag="IV329_001923"
     CDS             42..1160
                     /locus_tag="IV329_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8941093.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1288..2664
                     /locus_tag="IV329_001924"
     CDS             1288..2664
                     /locus_tag="IV329_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8941094.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3065..4645
                     /locus_tag="IV329_001925"
     CDS             3065..4645
                     /locus_tag="IV329_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8941095.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4843..5066
                     /locus_tag="IV329_001926"
                     /pseudo
     CDS             4843..5066
                     /locus_tag="IV329_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5271..5591)
                     /locus_tag="IV329_001927"
     CDS             complement(5271..5591)
                     /locus_tag="IV329_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8941096.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5739..7973)
                     /locus_tag="IV329_001928"
     CDS             complement(5739..7973)
                     /locus_tag="IV329_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8941097.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
BASE COUNT         2506 a         1440 c         1621 g         2568 t
ORIGIN      
        1 gaatataata tatactagta agaaagaaag gaggaaatct attgtttgaa aaacttagac
       61 agtctaaact aatgttttgg tcgcttgaat tattgatcat agctgcattg atcatgattt
      121 catcagaaat aaactttatt ttcgaaccga tcgggacatt tttttccacg ttatttgcac
      181 ctgtactgat tgcaggcttt ctttactatt tattgaatcc tattgtcaag ctgttgatga
      241 aagcaaaaat caaaagaata tgggctgtcg caattgtgct gctcctttta gttgcagcaa
      301 ttatttggat tttacttagt gtcatcccaa gtctagtaca gcaaatttcc tcattggcat
      361 ctaatatgcc tgatttcatc aaacaggtag aaagctggtt aaaagaggta gccgaattgc
      421 ctttgttcaa ggaagtagat atcaataaat attttgagca attagacatt tcttacggga
      481 caatcattca gcaattcctt agtggagtat ccaatagttt aggttcgatc gttggaacga
      541 tcgcttcggc aaccgtagta atcattacag caccattcat cttgttctat atgttgaaag
      601 acggagaaaa actagtgcca aacatccaac agtttttccc agaaaaaaga agaaaccaaa
      661 ttgtggagtt attggggcaa ctgaataaaa cattggctaa ttacattagc ggacaagcaa
      721 tcgaatgttt attcgttggt accttcacat ttttaggata tatggtaatt ggtgtggatt
      781 atgctttctt atttggggtc attgcgggat tgacaaacct aattccatat ttaggacctt
      841 atctaggact agcacctgca tttctagtaa cagtcttcaa tgatccggta aaagcattac
      901 tttgttgtgt ggttgtgcta atcgtccaac agttggatgg gaatatcatt tatcctaatg
      961 taattgggaa atcactgaaa atccatccat taacgatcat tatcgttctt ctagtagctg
     1021 gaaatattgc cggattgctt ggtatcttct taggtgttcc tttttacgca atctgcaaga
     1081 cgatcatttc ctatataatc aaaatcgtca aagaagacaa gcaaaatgaa aacaagaaga
     1141 aaattgctac gacctcgtag gtagttttag tacacttgtt tttattgcaa agaaaattgc
     1201 tttgtgatac cattttaacg tggctatagt gccacgtttt tttctatcgt aaactaaaaa
     1261 ttaattttat aaggagagaa tgcgattatg aatgctgacc ctgagagtca gtcgctgtta
     1321 gcgcagattt tattattgat cgttttgaca ttgatcaatg cttttttagc agcttccgaa
     1381 attgctgttg tttcgatcaa taagaatcga atcgagcaaa aagctgatga aggagatgtg
     1441 aaatctcaaa agcttttaaa aatattgcag aatccaaata atttcctatc gacaatccaa
     1501 gtaggaatca ctttggtcaa cattttgtct ggtgcttcac tagctaatac tttatcggaa
     1561 cggttagctc cggtattagg tggcggcaca gcggcaaaaa atattgcaaa tatcattgtg
     1621 ctggctattt tgacctatgt atcgattgtt tttggagaat tgtaccctaa acgtattgct
     1681 ttaaataaat cagaagaagt cgcaaagttc acttcagggc tgatacgctt gattggtgta
     1741 gtagctaagc ccttcgtctg gctgctttct gcttccacaa gtttattggc gcgtatcacg
     1801 ccgatgactt ttgatgatga agattcaaaa atgaccagag atgaaatgcg ttatatgctt
     1861 gaaaacgaag gcgtattgaa taatgaagaa ttagaaatgc tccaaggggt tttttctctt
     1921 gatacgaaag ttgcccgaga agtcatggtc ccacgaacgg atgcatttat gattgatatc
     1981 aacgacactg tggaagaaaa tgtgaatgaa gttttatccg aaaactattc aagaatccct
     2041 gtttataacg aagacaaaga taaagtagtc ggaatccttc atacaaagaa tttactaaaa
     2101 gctgcccata aattcggctt tgataattta gatatcaaga aaatcatgca ggagccttta
     2161 ttcgttcctg aaacggtctt tattgatgat ctgttatatg aaatgaaaaa gacgcagaat
     2221 cagatggcta ttttattaga tgaatatggc ggcgtcgttg ggttagcaac actagaagac
     2281 cttttagaag aaatcgtcgg agaaatcgat gatgaatcag atgaagttga gaatctttat
     2341 gaaaaaatcg atgaacacga atatatcatc caaggacgta tgctgattga tgagttcaat
     2401 gaagcttttg acagtgatct gcatatgagt gatgtagata cgatggctgg ttatttgatt
     2461 acagcattag gcatgattcc ggatgaaggc gaaaagttat cgtttgatgt cgataatatt
     2521 acgctggttt ctgaagaaat ggaaggttct cgaatcctta aaatccgtgt catttttcat
     2581 gatccggaag aaacagaagc ggaacctgaa gaagaacgaa gatacttcag aaaagaattc
     2641 gaagatgacg agccaagaag ataattggaa acttggttat gaaagacgtg ttgcttgaat
     2701 aaacaagcaa cacgtctttt cttttagtca gctgacaaaa aaatatttga ctcttatggt
     2761 ttggtggctt tttcattcgc tgaatccatg ctatactgat tcagttgtag tagttgacaa
     2821 aggttgtgaa aaagctatcc tgcattaagt aataagagca gccaagcaaa aacagctttt
     2881 cgtatttttg gcttgggtgg gcttattgct gataggctgt ctctagaacg ccgtttactc
     2941 ggaaaaagag actgctacac ggcttttgtc acagccctat atgaaattgt ctttctttaa
     3001 gagatcggac aatctgtgcc tagagaaaat ataaataatt gaatggaaga aatggagtta
     3061 ggttatgaat aatccaaaat taaagcaaca agtggacaat cgcagaactt ttgcgatcat
     3121 ttcccatccg gatgctggga aaacaacgat tacagaacaa ttactattgt ttggtggggc
     3181 tatccgtcaa gccgggaccg taaaaggcaa aaaaacaggt aattttgcca aatcagactg
     3241 gatggaaatc gagaaacaac gtgggatttc tgttactagt tctgttatgc agtttgatta
     3301 tcaagggaaa cgagtaaata tcttggatac accaggacat gaagattttt ctgaagatac
     3361 ttatcgtact ttgatggccg tggatagtgc ggtcatggta atcgatagtg caaaagggat
     3421 cgaagcacag acaaagaaat tattccaagt tgtaaaaaaa cgtggcatcc cgatatttac
     3481 ttttatcaac aaactagatc gtgatggtcg tgaaccattg gaattgttag aagaattaga
     3541 agagctatta gatatcgaat cttatccgat gaattggcca atcggtatgg gtaaagggct
     3601 agaaggtctt tatgatatct accataatcg tgtagagttt tatcgtccgg aaaattatca
     3661 ggatgaacga ttagtagaat tggatgaaga tggactgctt cccgaaaatc atcctctaac
     3721 tgaaaattcc ttatatgaac aagtcttaga agaagtagaa ctaatcaaag aggctggaga
     3781 tgcatttaat caagaaaaaa ttgctcgtgg cgatcagaca ccagtatttt ttggttctgc
     3841 attgactaac ttcggtgtac agactttttt agagactttc gttgaattgg caccagctcc
     3901 ttatggacac aaaacacaag atgatcagat ggtaagtcct tatgaggaag aattttcagg
     3961 tttcgtattc aagatccaag caaatatgaa tccagcccat cgtgaccgaa tcgcttttgt
     4021 ccgaatctgt tctggaacat tcgaacgagg aatggatgtc tggttggaac gtacaaataa
     4081 aaaactgaaa ttaagcaatg tcacacaatt tatggcagat tcgagagaaa acgtcgaaaa
     4141 agcagtagca ggcgatatca tcggtgtata tgatacaggc aattatcaga ttggggatac
     4201 attgttcgaa ggaaaactaa aagttgctta tgaagaactc ccttcattta ctccagagct
     4261 gtttatgaaa gtgactgcaa aaaatgtcat gaaacaaaaa tccttccaca aaggaatcta
     4321 tcaattagtt caagaaggag cgattcagct ttataaaaca tatctaacgg aagaatatat
     4381 cattggagct gttggtcagc tacaattcga agtattccaa tatcgtatgt taaatgagta
     4441 taacgccgaa gtaatcatgt ctccaatggg acacaaaatc gctcgctgga tcgatccaga
     4501 agatttggat gagaagatga gttcaagtcg caatatcttg gctcgtgacc gtttcgacca
     4561 accgttgttc ttattcgaga accaatttgc agaacgctgg tttgcggata aatatcccaa
     4621 tgtaaaatta aaaagtttga tgtaaagaag tcttgacaca agctgccctg catcaagtaa
     4681 taagaatagc caaacaaaaa cagcctctca tattttgttt agagccgtga cggattttcc
     4741 gtgacggctc tttttactat attaaaatca tagaaatgtc taatcaaaaa aatataaaaa
     4801 ttattttgcg tacatatgat taaagaaagg agagtgtgac acatggaaac agaatggcaa
     4861 agacgtgaaa gaaaaaaaca gaaaaagaag agatgttacg attatgtcga ttattagatg
     4921 taaaagactg ggacaaaaac gagcaaattg cacaaaaagt acgatcaatc atccataaag
     4981 aggaaaaagt taaaaataaa gacacgcgta gaactgaaaa tgaaaagaga aaagtacaaa
     5041 agaaaagaga aaaatcaaaa agctagaaag aaaaagaggc tgagacagaa gcgtttaact
     5101 ccaagaaata agctggaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcagctta
     5161 tttccgaagg agttgcttct gctttcgccg tttatacgtg tttagagcgt gagacaaaag
     5221 tgttctttac ttttgtccca cgctctttaa ttattatgtt caaaatttgt ttacacggct
     5281 aatgtagcgc ctaattcatg ttttggaatc gagatttcaa caaggcctgc caattcgatt
     5341 actttgattt catcctcgtg gtaaacggcg tctttgattt tatctaagaa ataattttta
     5401 gcatcttcga tttctttcgg ctggatttta cgtttgtcat tgatcagcag aatttcatta
     5461 tgttcagggg cttcagtgta gacaatagtg gtatttccgg ctgtgtagac cttgactaga
     5521 tgggcatcag tgttttcaag ctggtttaat acaagacggg aatggctgtt tgttacattt
     5581 accaatttca tggcgctcac ctcttttatg tatttttaat tgcttgtttc tcttattata
     5641 atttcttttt tataaaatga acagtatttt gacttttctg aaaaaagaat gagacggcat
     5701 ttctcaaaga aataccgtct cacgctataa aactggattt attcagccgt ttcttcggct
     5761 gattctttag ccaaacgttc tggtttagaa gtaacgatga tttttccatc tttatccagt
     5821 tttgctttca actcatggat agaagggtga tcaagataaa attcagcgat accatcttcg
     5881 atttgttctt ggatcgtgcg tcgaagagga cgagcaccca tagatggatc atagcctaag
     5941 tcaactaatt tttctttgac attcgtagga acttcgatgt gaagttgctg tgctgccagc
     6001 atttggttga catcgtctag cattagtgtg acgattgtca tcaagttttc ttttgataat
     6061 gcactgaatt cgatgatccc gtcaaaacgg ttcagaaatt ctggtgtaaa gtagttgttt
     6121 aattggttca atacagaacg agtaacacct tcgcgtgccg caccaaaacc aacgtttgct
     6181 tctactttac cagttccggc attacttgtc ataatgatga tcgtatcttt aaagctgacc
     6241 gttcttcctt gtgcatctgt caatcgacca tcatctaaga tttgcaagaa catatgcaaa
     6301 acatctggat gagctttttc tacttcatct aacaagacaa gactgtaagg atttctccgt
     6361 actttttctg ttaattgacc agcttcttcg tatccgacat aacctggagg agaaccaatc
     6421 aatttagaga cactatgttt ttccatatat tcagacatat cgaaacgaat cattgaatct
     6481 tgtgaaccga atagttcgta ggcaagttgt ttcgcaagct ctgttttacc tacaccagtt
     6541 ggtccaacaa ataagaagga gccgattgga cgattctgtt tgtttaatcc aacacggttt
     6601 cggcgaatcg ctttcgctac tcgatctaca gcgttatctt gtccgatgac atgagctttc
     6661 aagtcatctg ctaagttttt cagttgtgtc tgttcttttt cttttaattc acccactgga
     6721 atacctgtcc gttgttcaac gattttttcc atatcttttt cggtaatcac aggtgtttcc
     6781 tcttcagtga gctgacgttc tttcattttt tgaagtttat tgatctgatc gcgataataa
     6841 gctgcttttt cgaaatcttc ttctcgagaa gctaaaactt tttgttcttc tgcttcttgc
     6901 agttttttct cgattgtttt aggatcaacg atttggatcg tcaagttttt cttagaacct
     6961 gtttcatcta gcaaatcgat ggctttatcc ggcaagaagc gatcttggac gtaacgattg
     7021 gataaagttg cggctgcttc gatggcctca tcagtatatt ttacatgatg atagtcttca
     7081 taacgttttt gcaaaccttt caaaatactg atcgtttctt cgacagttgg ttcatctacc
     7141 cgtacaggtt gcatacgacg ttctaaagca gcgtcttttt cgatgatgcg gtactcattt
     7201 aaggtagtag caccaaccat ttgcaattct cctcgagcta aagctggttt taaaatatta
     7261 ccagcatcca tatttccatc accagcagaa ccagcaccga cgatttcgtg aacttcatct
     7321 atgaatagga tcacattttc tgcttgacgg atctcttcaa tcaatttttg catacgttct
     7381 tcaaattgtc ctcgtatacc agtaccttgg actaaagata cgacatctaa acgaatgact
     7441 tctttgtcca ttaatttttg cggtacatca ccatctacga ttttttgtgc taaaccttca
     7501 acaactgctg ttttaccaac acctggttct ccaatcaaga caggattgtt cttggttcgg
     7561 cgatttaaaa tttcaatgac tcgtttgatt tcgtcatcgc gtccgatcac aggatcgata
     7621 tcaccttgtc gtgcttgttc tgtgatattg attccgtatt ctcccaataa accttctgta
     7681 ttgccttgag gtggctgacc accattgaag ttgttgtttc cgccaaattg tgttggtggt
     7741 gtttgctcat aatttccttg ctgttgcatt tggcgagaca tggaacggaa aagatcgtcc
     7801 aaactgccaa atccaaatgg atcattttgg gccatattag tcagacctcc attttgttga
     7861 tttttgattt tttgataaca gctctgacag taatcgagtt gtgtccgctg accattgaca
     7921 gtggcataca agtgaatcgt tgcctcattc ttgccgcagt tttgacaaag catgaatcat
     7981 tcacgtcctt tcacaaaaat gggtacatcc atattttaca tctcgttttt catgaggtaa
     8041 aagaataacc cctagttttt taagagattt atgaggtaga aaaggtgaaa tctctattga
     8101 ccattcctga cctttgactc ttattatact aaaaa
//
LOCUS       DACDKL010000112         8013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDKL010000112 DACDKL010000000
VERSION     DACDKL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.112"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            384..572
                     /locus_tag="IV329_001929"
     CDS             384..572
                     /locus_tag="IV329_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8941098.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="IV329_001930"
     CDS             650..1663
                     /locus_tag="IV329_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8941099.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="IV329_001931"
     CDS             complement(1730..2764)
                     /locus_tag="IV329_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8941100.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="IV329_001932"
     CDS             2879..5104
                     /locus_tag="IV329_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8941101.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="IV329_001933"
     CDS             5122..7005
                     /locus_tag="IV329_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8941102.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="IV329_001934"
     CDS             6986..7960
                     /locus_tag="IV329_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8941103.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2585 a         1463 c         1698 g         2267 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attgcggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga caaagctgct tta
//
LOCUS       DACDKL010000113         7824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDKL010000113 DACDKL010000000
VERSION     DACDKL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.113"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..787
                     /locus_tag="IV329_001935"
     CDS             86..787
                     /locus_tag="IV329_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8941104.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1396..2412)
                     /gene="tsaD"
                     /locus_tag="IV329_001936"
     CDS             complement(1396..2412)
                     /gene="tsaD"
                     /locus_tag="IV329_001936"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8941105.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2409..2873)
                     /gene="rimI"
                     /locus_tag="IV329_001937"
     CDS             complement(2409..2873)
                     /gene="rimI"
                     /locus_tag="IV329_001937"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8941106.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2880..3422)
                     /gene="rimI"
                     /locus_tag="IV329_001938"
     CDS             complement(2880..3422)
                     /gene="rimI"
                     /locus_tag="IV329_001938"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8941107.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3406..4230)
                     /gene="tsaB"
                     /locus_tag="IV329_001939"
     CDS             complement(3406..4230)
                     /gene="tsaB"
                     /locus_tag="IV329_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8941108.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4319..5299)
                     /locus_tag="IV329_001940"
     CDS             complement(4319..5299)
                     /locus_tag="IV329_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8941109.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5323..6807)
                     /gene="galT"
                     /locus_tag="IV329_001941"
     CDS             complement(5323..6807)
                     /gene="galT"
                     /locus_tag="IV329_001941"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8941110.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6819..7808)
                     /gene="galE"
                     /locus_tag="IV329_001942"
     CDS             complement(6819..7808)
                     /gene="galE"
                     /locus_tag="IV329_001942"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8941111.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2186 a         1694 c         1389 g         2555 t
ORIGIN      
        1 ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag
       61 tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa
      121 actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga
      181 acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag
      241 acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc
      301 aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt
      361 tggagaagag acctacactt atacagctca agcgatgact gatattgaaa cattctacct
      421 tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat
      481 acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc
      541 tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc
      601 aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa
      661 tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct
      721 gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag
      781 agaataacgc gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt
      841 tcctccctaa aacttctgaa agatccggca gttttagatc actggacaag actcaaagat
      901 tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa
      961 ctagatgaag cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac
     1021 gattactcag tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc
     1081 aaaataacaa taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca
     1141 gccctatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc
     1201 ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc
     1261 ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca
     1321 ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc
     1381 tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca actgatagct
     1441 agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc
     1501 tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag ctgccaaagc
     1561 atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg
     1621 cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact
     1681 tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt
     1741 atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc
     1801 atggatcatc gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat
     1861 ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc
     1921 tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac
     1981 taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac
     2041 cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg
     2101 tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg
     2161 tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc
     2221 atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac
     2281 gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt
     2341 tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa
     2401 ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc
     2461 gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa
     2521 agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt
     2581 atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa
     2641 actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag
     2701 ccacagatct ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct
     2761 gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc
     2821 catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct
     2881 cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat
     2941 ctttttttga tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga
     3001 ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg
     3061 atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata
     3121 ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca
     3181 ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga tataaaggca
     3241 ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg
     3301 atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt
     3361 tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac
     3421 ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt
     3481 taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg
     3541 agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg
     3601 caatatttcc tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc
     3661 ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag
     3721 tacagtttct aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg
     3781 agcatcaaac atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt
     3841 cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt
     3901 tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat
     3961 gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac
     4021 actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac
     4081 accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct
     4141 ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat
     4201 accaaaggca aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc
     4261 ttcctgaaac acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt
     4321 atttcgttga ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg
     4381 ctttggaatt acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat
     4441 aaacttttac agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc
     4501 ccatcacaga aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc
     4561 caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta
     4621 aatattgctt cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac
     4681 cttcgtcgta gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta
     4741 gccgctgatg gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt
     4801 tatgggagac aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag
     4861 agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt
     4921 tcatttctaa taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa
     4981 gatcactaat ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt
     5041 tttcgattcc taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt
     5101 accactggat caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat
     5161 aatttaagcg ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat
     5221 caggatcata gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa
     5281 tgtctttaat tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa
     5341 tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac
     5401 acgtgcaaaa acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa
     5461 atcaggatgt tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt
     5521 ttcttctcct aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa
     5581 aacagcaagc cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc
     5641 tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac
     5701 aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg
     5761 tgtaccatct gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt
     5821 ttccaatact gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat
     5881 cacagacatc ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat
     5941 aagtttttct atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc
     6001 atgagaaaga atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg
     6061 aggcaagact tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg
     6121 gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg
     6181 gaatccccag ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg
     6241 atagttcaag cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc
     6301 tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa
     6361 attgattgtg atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc
     6421 gatggctctt gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc
     6481 ttcctctggg cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg
     6541 tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag
     6601 aacaccattt tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt
     6661 aactggtctt acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt
     6721 ctgtaaatat aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa
     6781 atcacaaatc gtttgactga ttgacatatc tcattcccct attcttcata accatttgga
     6841 tggctgacat gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt
     6901 ttccagccca gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca
     6961 gctcttcttg gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct
     7021 agcatttcct tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt
     7081 ccttctttca agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca
     7141 taatctctga tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct
     7201 tttctttgac caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga
     7261 tcttctccga tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca
     7321 tagcgcatgc catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt
     7381 tcaccatacg gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc
     7441 tctccataag ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg
     7501 acttcaagta agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg
     7561 acagattctc ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt
     7621 tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct
     7681 ggatgtactg cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc
     7741 ttttgaacta gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa
     7801 attgtcataa aaaacactcc tttg
//
LOCUS       DACDKL010000114         7785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDKL010000114 DACDKL010000000
VERSION     DACDKL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.114"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            113..388
                     /locus_tag="IV329_001943"
     CDS             113..388
                     /locus_tag="IV329_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941112.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            401..2473
                     /locus_tag="IV329_001944"
     CDS             401..2473
                     /locus_tag="IV329_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8941113.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2638..4524
                     /locus_tag="IV329_001945"
     CDS             2638..4524
                     /locus_tag="IV329_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8941114.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4536..5483
                     /locus_tag="IV329_001946"
     CDS             4536..5483
                     /locus_tag="IV329_001946"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8941115.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5503..6030
                     /locus_tag="IV329_001947"
     CDS             5503..6030
                     /locus_tag="IV329_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8941116.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6093..6746)
                     /locus_tag="IV329_001948"
     CDS             complement(6093..6746)
                     /locus_tag="IV329_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8941117.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6878..7720
                     /locus_tag="IV329_001949"
     CDS             6878..7720
                     /locus_tag="IV329_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8941118.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         2658 a         1316 c         1654 g         2157 t
ORIGIN      
        1 aaaaatttat ggaaatttct aactagcaat aaacgatgaa acaatgaaat atttttcatc
       61 caaaaagaac tttgctataa tgatggaagt ttgtgtatag gagatctgga aaatgagaga
      121 gagttttgaa caacaaaaaa aactgttgca tgatcgctat ggcgcccttt cgatggatga
      181 ccgccgtcaa atactttgta agttgcgtaa aagaaatata ttgatgtatc gtcagttgga
      241 acgtctgaaa catgatctgc ttcgtttgga gtcaaaacgt gtacagtgtg aattagaggg
      301 gaaccaaaca caggttgaag tagtcgaaac caaaatttta aagaaaaaag aacaattttt
      361 aaaaatgctt acacaaaata aaaaataaag gggatggacg atggaatttg ctgagttagt
      421 tattgtattt gcagtaacga tcaccttatc gaacatagct agccgcattt tgccaatgat
      481 tccagctcca ctgatccaaa ttttccttgg agtggtatta ggtctgacag agtggggaca
      541 atcaatcgat tttgaacctg aattgttctt agttatgatt attgcaccat tgttatttag
      601 agaaggagaa aaagcagata tttcttcgat tctcaaaaat ttcgggacaa ttttgttctt
      661 ggcatttgga ggagtcattt tgacccttgt cggggtaggt gcgacgttat cattcctgtt
      721 gccaagtgtc ccacttgctg cctgctttgc ttttggagca gcgttaggtc cgacagatgc
      781 ggtagcagtc agttctcttt ctggacgagt gaatatccct aaaaaggcga tgcatatttt
      841 ggaaggagaa ggtcttttga atgatgcatc tggtgttact gccttccagt ttgctcttgg
      901 tgcgctgatc accggatctt tttcagcagt aaacgcgggg atgagtctag tagtttctag
      961 tattggggga gctttgatcg gctttttgct tgtctggttc aaacaaaaaa taattcattt
     1021 gatcgaaaaa gcatcagcac aagatgtcac agggtatctg ctgatcgaac ttttgcttcc
     1081 ttttctagcc tatgtattgg cagaggcatt tgatgtatct ggaatcattg ctgcagtggc
     1141 agcaggtatt ctccaagctt ctggttttag gaaaatctct gtgtttgatg cagaattgtc
     1201 gagcttgtcc cacagtacat ggacaactat tgcttttaca ctgaatgcat tagtatttat
     1261 atttttagga atcgaattga cgcaagtgtt ctcgccagta tggggagacg gtttatatcc
     1321 aaacggtctt ttattagcga ttatcgtatt aatctctgtc atgctttttg tgatcagatt
     1381 catttctatc agtctttttt atgtattcaa agatggcagt aaaaagttca aaaaacagtt
     1441 gaacgagatc ttgatcttaa catttggcgg tgtaaaggga acagtcagtt tggctacgat
     1501 ttttattttg ccattttcaa tcaataatat gatgttttac cagcggtctt tgcttttgtt
     1561 tctgacggcg ggtgtcatct tagtgacttt ggtcataggg atcattgtct tgcctatgct
     1621 gacggaaaca gaggaagcga aaagcacgga tttgaatgct ttgatgattt tagaagaggt
     1681 cgttgaagtt ttgcgaaaag aaataaagga aattgatcgt aatacaaaag aatttttagc
     1741 cactgaagca gtgatagaaa attatcaaga acggatacgt gatctgtact tggaagattt
     1801 aaccgatgat gaaaagcaag aagtacaaga aatacaagca ctgattttgt cgatcgaacg
     1861 ggatggattg gatgaaagtt atcgttcggg caagctttct tctaatggtt atcgttttta
     1921 ctcccgtttt ctttctcgat tcgaacattc tatcacaagc cagattttgt cttttatcgg
     1981 cttttggttc attgttgtcc gtaggttaat gcgaatcgtt ctccatccga aaatgttttg
     2041 gcaaagaaga tatgctgatc gtcagacatt tattagtgat gaagatatcc aagaaatcag
     2101 agagacatat caaaaaaata ctcagttgat tattgaaagt ttagataact tgactgatat
     2161 ttatgatgaa acgatggttc aattttttat ccagcaacgc aaacttgaag gaataaaaat
     2221 gaactcgggg aatttgattt cttcgtggat gatccagcaa gatagtctgt ttacgaaaaa
     2281 aatgctaaga ggttattatc ttgaaagaaa aatcattgat gaatacgaag ttgctgaaaa
     2341 aatcacgacg ttttctgcga acagctatcg caggaatatc aatttgttag aatcttatac
     2401 gatgaacaaa ccatctgata acttttcttt ccgatttgcg tttcgagcaa agtcaaaaaa
     2461 acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc
     2521 agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa
     2581 aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg
     2641 ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac
     2701 cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg
     2761 ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa
     2821 acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc
     2881 gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt
     2941 cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa
     3001 aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc
     3061 aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact
     3121 ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat
     3181 ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa
     3241 aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta
     3301 gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga
     3361 aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa
     3421 gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca
     3481 cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac
     3541 caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa
     3601 gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta
     3661 gatttattga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa
     3721 tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc
     3781 ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa
     3841 cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa
     3901 atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg
     3961 ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag
     4021 caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg
     4081 attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt
     4141 tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa
     4201 ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct
     4261 gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac
     4321 atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc
     4381 tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg
     4441 caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac
     4501 ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag
     4561 gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa
     4621 gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga
     4681 aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga
     4741 atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct
     4801 acatcaaaag tccagactat aatggaccag atatgactga ttttggccat agatgtttga
     4861 cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc
     4921 taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat
     4981 ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag
     5041 caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg
     5101 ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac
     5161 ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca
     5221 ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg
     5281 aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag
     5341 aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt
     5401 cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga
     5461 tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg
     5521 gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat
     5581 gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca
     5641 ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat
     5701 tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg
     5761 gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt
     5821 ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct
     5881 tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg
     5941 gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta
     6001 aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta
     6061 gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac
     6121 ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa
     6181 ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata
     6241 acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc
     6301 ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac
     6361 gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct
     6421 agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa
     6481 atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga
     6541 aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca
     6601 atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata
     6661 ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac
     6721 gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa
     6781 aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga
     6841 ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac
     6901 agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc
     6961 gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag
     7021 atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg
     7081 agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca
     7141 tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa
     7201 aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat
     7261 tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa
     7321 aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa
     7381 aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt
     7441 gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt
     7501 cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat
     7561 cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga
     7621 attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac
     7681 tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct
     7741 cagcctgaaa ggcttgcagc ttattctctt tttctttata aaatt
//
LOCUS       DACDKL010000115         7732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDKL010000115 DACDKL010000000
VERSION     DACDKL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.115"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..367
                     /locus_tag="IV329_001950"
     CDS             <1..367
                     /locus_tag="IV329_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941119.1"
                     /translation="YYRYLDEFYRIFASKLSSILSDKINVKKSFFKKETPVEKISVEL
                     LKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSV
                     TDRHWQLVEDADLSYLPLK"
     gene            complement(448..1017)
                     /gene="thiT"
                     /locus_tag="IV329_001951"
     CDS             complement(448..1017)
                     /gene="thiT"
                     /locus_tag="IV329_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8941120.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1083..1185)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1191..2162)
                     /locus_tag="IV329_001952"
     CDS             complement(1191..2162)
                     /locus_tag="IV329_001952"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8941121.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2256..3629)
                     /gene="glmU"
                     /locus_tag="IV329_001953"
     CDS             complement(2256..3629)
                     /gene="glmU"
                     /locus_tag="IV329_001953"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8941122.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4132..4950)
                     /gene="purR"
                     /locus_tag="IV329_001954"
     CDS             complement(4132..4950)
                     /gene="purR"
                     /locus_tag="IV329_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8941123.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5117..5941)
                     /locus_tag="IV329_001955"
     CDS             complement(5117..5941)
                     /locus_tag="IV329_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8941124.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5914..6594)
                     /locus_tag="IV329_001956"
     CDS             complement(5914..6594)
                     /locus_tag="IV329_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8941125.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6617..7555)
                     /locus_tag="IV329_001957"
     CDS             complement(6617..7555)
                     /locus_tag="IV329_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8941126.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
BASE COUNT         2214 a         1675 c         1342 g         2501 t
ORIGIN      
        1 ttattatcgc tatttagatg aattttatag gatttttgct agtaaattaa gttcgatctt
       61 gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg ttgaaaaaat
      121 cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag gaagggatca ttttttcttt
      181 tgatcaagtt ggtttgatcg atggtaaata tcgtatccca atctatgtga ttgaagatta
      241 tatttggaaa ttaaaaggca cccggtatcg agaagaggaa atcatcgcta ctttaatcta
      301 ctctgtcacc gatcgacact ggcaattggt agaagatgcc gatctttctt atctgccatt
      361 gaagtagatc tatttcttat caaaaaataa aaaggaaaca agaaactagg acatgattct
      421 gtctcaagtt tcttgcttct tttttattta cagctcctgt cgtttatctg gcacaaaaat
      481 cgaaggagtc gtttgttgga tccctaataa aacgatgatc gttacaatag ctgtactcaa
      541 cccgctggca ccattcatta cgaaagaaaa gagccaagga ttcatccccc atagtgcata
      601 cgctccccag aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa gtgcgccaat
      661 caatgcacca aacacgatgc tttttctaat ttgtttcgta ttatcgtcta catagccttt
      721 ttggacattt gaagccatga gccctgctgc gcctgcaaaa gtataggcaa tcgggtactc
      781 aatcaacact tgaacgacac ttaaataata aacttgtccc aacggaaaat gtaggatgcc
      841 ccaaatgaaa cctgccagca tccctgcctt tgtccctctt cttaacgcaa aaagagtcaa
      901 tggaatctgc cctaatgaaa tagaaaaact gctgccgatc tggatcggaa tcaacgacaa
      961 caccatcgct aacgctgcaa caatcgttcc ttctacccaa actctatttt ttcccataaa
     1021 aaaacttcct cctttgaata aaaaagtcac aaaggaggaa gcagctttcg aaaatagtca
     1081 aacagctgtt tattcacaat tcctacgctc gcataaccga tacaggtacg aagggtttag
     1141 aaatagattc aatctcagcc caattggact cccctttgtg agtttattta ttatttattt
     1201 tttgtttcaa acaatggact aactggtttg ttttcatgga tacgtttgat tgcatctccc
     1261 attagtccgc caacacttac ttcatcgatt ttgtcgattt tacgatcttc tggaagatag
     1321 atcgagtctg taacaactag acgctcgata gctgaatctt ctattctttg taaagcaggg
     1381 ccggataata ctggatgtgt acatgaagca tacacactag ttgcaccagc ttctttcaaa
     1441 gcattggcag ccaatgtgat cgtacctgct gtatcgatca tatcatcgat cagtacacaa
     1501 gttttgcctt tgacttgtcc aatgatattc attacttctg caacatttgc ttttggacgg
     1561 cgtttgtcta tgatcgcaat cggagatttc aaaaattccg ctaatttacg tgcccgtgtg
     1621 acaccaccat ggtctggtga aacaacgaca acatcatcgc ctttgattcc tttttctaag
     1681 aaataattgg caataagtgg cgcacccatc aaatgatcga ctggaatatc aaagaaacct
     1741 tggatttgga ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc tttttgaatc
     1801 atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg gtcttgacgc
     1861 gcatagccat aataaggcat gactacgttg attgtttttg cacttgctcg tttcagtgcg
     1921 tcaatcatga tcagcaactc catcaagtta tcattgactg ggctgctcgt tgattgaata
     1981 acatagacat gcgatccacg aatactttct tcaatgttta cttgaatttc tccatcacta
     2041 aattgagtaa cagatgattt tcctaactcg acgccgactg ctgctgcaat tttctctgct
     2101 aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta atgttttgac
     2161 atggggacct ccactttctt tttcacaact ctagaattct acctaataaa tagtagtcat
     2221 attttttaaa taaatcaagt gattttgcaa aagtcttagt ttagataagg gagttttttt
     2281 gcatatcctt ctttattgac ttgtcttgct ctcgcaattg ccagcgcata ttctggaata
     2341 ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt tagctacctc gacaggtccg
     2401 atgatattcg tgctagaacc aataaagctg tggtcgccga cagtcgtatg atgtttgttc
     2461 tttccatcat agttgacgaa gacaacacca catccgacat tgatttcttc tcccagcgta
     2521 gcatcaccaa cataagtcaa gtgaccgact ttagttcctt tgccgatttg agcatttttc
     2581 acttccacaa agtttccgat atggacgttt tcgccaattt cagcttttgg tcgtaaatgt
     2641 gcataaggtc ccacatctgc atgtttcttg acatggctgc tttcaatgac tgagttttca
     2701 atgacgacgt gatcctcaat cctactgtct acgatttttg aatgtgaccc gatgacacaa
     2761 tcagaaccaa tgactgtatt tccttggatt tgtacacctg cttcgatcaa tgtgtctgga
     2821 ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct tacgccattg
     2881 accatatgca tctggttaat tcgctttctc atgatctcat ttgctttagc taacgcgatc
     2941 cgatcattga ctcccattga ttcttcaaaa tcatccgttt gataagcagc aactgtatgg
     3001 ccttcttctt ttaagatttc gatgatatct gttagatagt attcaccttg agcattgttt
     3061 gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta agttcctgta
     3121 ttgatttctt gtacaagagc ttcttctggt gttgcatctt tttgttccac gattttttct
     3181 acgatcccaa tatggtcacg gatgattcgt ccatatcctg ttgggttttc tgcttgtgcc
     3241 gtcaagatcg ttgcacttgc attttttcct tggtgatatt caaaaagatt gttcagcgtt
     3301 tcagtagtca ataatggtgt atcgccactg ataactaacg ttgttccttt ttttccttcc
     3361 aagaaagaag cagcttgcaa aacagcatgt cctgttccca attgttctgc ttgtaacgca
     3421 tatttactgc gttctcctag ttgtgcttta acttgttctg ctccgtgtcc aacgatcgta
     3481 atcacttcat ctggtttcgt ttcactgacc cgattaatga tatgttctac cataggttta
     3541 ccagaaacgg gatgtaaaac tttatataat tttgacttca tgcgggtacc tttccctgcc
     3601 gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag tttgctgatt
     3661 tcagtttatc ccagtatacc cattttttca acaaaacaag tgattttctt ttatcccagc
     3721 gttttctgga taaaaaaata gatagaccat aacgggccta tctatttggt aaccggtgct
     3781 ctcctgccaa ctcctcggtc acaagtcaca ctactcataa aacatgaaaa gctgtttttt
     3841 gaaatcgttt cttgtgactt agagttaaac ggtaggctta caacctcttc attaaacgtt
     3901 tttcaatcag ctgaccttcg gtattagctc aataaacggc aaaatatgag gagcaatttt
     3961 tccgtttatt gagctaatac tcaggacagg acgctgattt cacaacctct agataaacgg
     4021 tgttcaaaag ccagtttctc ggtattaagc caaatttatg aaaaaatgtg gaaagccatt
     4081 ttcacaaaat ttttcttact actcgaaact aagcggcttt ttcacaacct cttattctga
     4141 aaaataattt cctggcacaa ccgtaatatt tttcgtttga gtatctacat ctttgacata
     4201 aagcagtgac gtataatcat cgatggcgcg atgaccgttg aattttgatt cggcaaatac
     4261 agtgatgcca acaagttctg attcgaattc ttcaatcaag ctcttcattc cgttgactgt
     4321 tccgccgcct ttcatgaaat catccactac aaggacacgt gaaccacgtt tcaaacttct
     4381 cttagacagt tccatctttt caattctttc agaagatcca gacacatagt tgacacttac
     4441 tgttgatcct tctgttattt ttgagtcccg gcgtacaata acgaatggcg cattcagata
     4501 ataggacaca gcttgggcaa taggtacgcc ttttgtggct acagtcatta cagcatcgat
     4561 tttttcacca aggtatttag aagcaatgat tcttccaact tgacgcagca gttcaggttc
     4621 tcctaacaaa tcagagagat aaacatatcc tcctggaagc agacggtctt gttctgatag
     4681 acgttctgct agagattcaa tatatttttt cgcctcttca tatgaaattt cagggatgaa
     4741 caatacacca cctgcagcac ccgggattgt ttccaaggtt ccataccctc gttctttaaa
     4801 tgttttcttg atgattccca aatcttcact gatagaagat ttagcagatt cataacgttc
     4861 ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc aataaatact gtgtcatgtc
     4921 aactagacgt tcactgcgac gtactttcac ctattccaac tccttttaag aaattttatc
     4981 gttttacatt atataagttt ttcttcaaaa attcgagcat tttatgaaaa aataagaaaa
     5041 tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc
     5101 ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga
     5161 tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat
     5221 ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga
     5281 tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca
     5341 tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag
     5401 taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat
     5461 cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg
     5521 caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa
     5581 acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg
     5641 ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga
     5701 tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg
     5761 taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg
     5821 tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc
     5881 cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca
     5941 ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc
     6001 atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag
     6061 acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa
     6121 taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga
     6181 caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg
     6241 tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc
     6301 tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg
     6361 aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat
     6421 tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc
     6481 attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag
     6541 tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca
     6601 acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga
     6661 cagaaatata attttcgcct gctttgattt gttcattagt caagctttct aaaggattca
     6721 acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat
     6781 tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta
     6841 agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt
     6901 cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg
     6961 catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat
     7021 cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa
     7081 tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat
     7141 gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac
     7201 ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta
     7261 acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa
     7321 tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca
     7381 aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa
     7441 aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg
     7501 ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata
     7561 tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa
     7621 aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg
     7681 attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta ca
//
LOCUS       DACDKL010000116         7718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDKL010000116 DACDKL010000000
VERSION     DACDKL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.116"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            92..2419
                     /locus_tag="IV329_001958"
     CDS             92..2419
                     /locus_tag="IV329_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8941127.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2545..2874
                     /locus_tag="IV329_001959"
     CDS             2545..2874
                     /locus_tag="IV329_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8941128.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2914..3918
                     /locus_tag="IV329_001960"
     CDS             2914..3918
                     /locus_tag="IV329_001960"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8941129.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3915..4361
                     /locus_tag="IV329_001961"
     CDS             3915..4361
                     /locus_tag="IV329_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8941130.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4511..5326
                     /locus_tag="IV329_001962"
     CDS             4511..5326
                     /locus_tag="IV329_001962"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8941131.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5532..6704
                     /locus_tag="IV329_001963"
     CDS             5532..6704
                     /locus_tag="IV329_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8941132.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6866..7360)
                     /locus_tag="IV329_001964"
     CDS             complement(6866..7360)
                     /locus_tag="IV329_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941133.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2606 a         1458 c         1531 g         2123 t
ORIGIN      
        1 cccaaacttt ctatacatca ataaaataat aaaaattaaa tttagaataa cgtgtaaagg
       61 attatttgat gattttttat tgagaggagg gatgaaaatg attaagaaaa ctcaaaataa
      121 aatccaacgt atatgtcaaa aacattttgt ttctagactc aaagctgtat ctaaaagaaa
      181 atacttacat cccattatga tttcgatcat cttgattttt actttcttag ggagcttatc
      241 aagtatttat gtaaacgttt atacagaaac gtcaaatttt gctaaccaaa cgacagagga
      301 gacaaatagt ccaccctatt ctgaaataca gtccatagaa agcaccaatc agtctagcaa
      361 caacgagaca acctcaacca catcctcgac tcaactgatt gatccgacag aaaaagaaat
      421 cacagcagca aaagcagcag gggagaaagc ttattcagaa acaggtagaa gccagacatt
      481 tagtactgtt gcggccactg cgaatttcaa cggacatacc ataccagatg gatatacagt
      541 gatatatgcc aaagatggag tcattgttgc ggttaacgga tcgatgagtg cggataaaac
      601 tgcgttacaa ggcgtaacca aagcaatcgc ttttattgat aaccgtccag atatccataa
      661 tctgtttatt ggctccacag taaatggtac tactatcaca ttcccaaaaa gtatctatat
      721 gggggtggct accgctggaa atacgatcga ttcagttaca cccaatgcgg ataaaagtta
      781 ttcttttaca ccctcaacat ggggagggct tcctgccgga ggaggtatga gtactttttc
      841 aatcggtata cagttagcaa atgatattcc attcacgggg tactcgatca aaaatgggca
      901 gatcgttgat ggtaagggaa atgtttcaaa ttttccaacc tatcaagcag caacggttca
      961 ggtcaatgcg aatcggacag gaactttttc ttatacagtc aatggtggag cagcaaaaat
     1021 cggaacgtac gggactacat tatctggaat ccatgtgggt gacaaagtgg ttatcactcc
     1081 aacagcagta acgggctatt cttttgcaca aacaaacggt tctttcacag ccctagaaag
     1141 taacgtcgat acggtgacct atacaggcaa acaaacacca atcactatta agtatcaaga
     1201 ctattttgga cagatgattg ctccttctaa aataatgaat cttacttacg gaagtgctgc
     1261 acaagacctg acgactaata cgcctactat caacggatat acctttacag ctgtttcagc
     1321 gatagaaaca aagaaccaat cagcaatgag tgttttagct agtttagata caaatggaaa
     1381 tgtaatcgta aaggacgcaa atggaaaaca gatttctgaa gtcatcctat attataagac
     1441 aaatgcaaca gtatcaatca atgcaaatgg cagtaagtac tatgatggtc tttctgttgt
     1501 accgatcgtt aaatatgcct ttaatgataa aacagaatca acatcccttt taaacaatga
     1561 tctcagtatt gatcaaatca catggaatcc aactgatttc aaagcgatga atgaaaaggg
     1621 agttgaagta actgcgccta ctgaaattgg aacctatacg tcttggcaat tgacaaaaag
     1681 cggactagct aaactagctg ccagaacaaa ctatttattt actatcgtgc agacatctga
     1741 tgtctatacg attaagcaaa tcagcggtgc ggtagagctc agtggcagta agacatatga
     1801 tggaaaagca ggtgtcccgt caatccatgt caagctagca gagggtgtca ctagttcgct
     1861 tactccagta caagtggcac ttagcagtat ggattacaca gcagatgcac aatcagcaaa
     1921 aaatatggtt aatgctggca gctatacaat taaactaacg aatagcggaa tagacaaagt
     1981 caaagcagca aatcccaagt tgtcatttac tgatatagcc aacacatcag gaacttatac
     2041 catctataaa gctaacgcag tgattactgt cgatgacgct gcctttaatt atgatgccca
     2101 aagtcactcg atttcggttg gaaatctcca cgttaccggt gttgtcttag gcgaatcatt
     2161 ggattatacc ctaacagtta acagccggac agatattgga tcacaaatag taacgattag
     2221 tttaggcgaa ggaagtgtaa acaataatta tacgattatc gcaacagata ccgctaagtt
     2281 gacgatcaat ccgactctct tgcttccttc gacaggtggg ataggtctac tgccctttgt
     2341 ctttttagga ctgatcttta ttttttctgg cttcttttat tttatccatc gtaagaaaaa
     2401 agcaggtgaa cagcgatgaa aaaaacaaaa aatgtacgtg tggatatatt tgctgttctc
     2461 ttttttatcc tactggtctt ttttataggt tctccgactg tgtcagctag agaatcacct
     2521 attacattta ctttgcataa tcgaatgttc gaaagcgata agatgccaag tatcagtcaa
     2581 aacgacggaa taaatatccc ggataacttt caaggagtaa aagggttgaa cggcgttact
     2641 ttcgctatat atgatgtctc ggatgaattt tacaagctgc ggtcagaagg aagtagtgtg
     2701 gaagacgcac aaagaaaact agcacaaaaa agtgatagtg aaaaaatact ggcagaaaat
     2761 gtgacaaaaa cagtagacga ggaagagggc attgcttctt ttagtgcatc ggacaaagat
     2821 gagcaaggtc gtgacgctgt ttaccgattt acggaaataa agacttcaga ttaagtaaaa
     2881 gaaaaatcag caccatttgt cgttgtgctt cctgtgacag attcatctaa tcataaattg
     2941 acgaccatcc acttgtatcc aaagagcgaa caaaaaatac ctgctgcact cacattaaca
     3001 aaaacagtag agaataagca gacagatttt gcagatgggg ataaagtccc ttatctcatc
     3061 accgtaacaa ttcctgagaa catcaacgag ataggaacct atacgatcaa agatacagct
     3121 gatcctcagt tatggctaga accagaaacg atcgattttt tgattggtgg agacaagatt
     3181 catacatttc acacacaaaa aataaaacat ggttttgtct tatcagattc tggaaaagat
     3241 cttagttcgt ttgctggaaa aaaattaacg atccgttacc aaatgacatt gaaagaaaac
     3301 agtgaaaaaa cgacatttga taacgatgta cgtttgacga ctgacaatca aacagtagaa
     3361 acacatcttg ccatccaaac aggaggcaaa caatttgtca aagtggatcg gcaaacgaaa
     3421 caaaaactag cagatgctca atttctagtc aaaaaaaatg accaatactt agcgcaagaa
     3481 aacgggataa atcattgggt agccaaagat gattcaagag caacgatttt tacttccagt
     3541 aaagatggaa cattttctgt ccatggattg tcttttggaa gttacgaact agtggagatt
     3601 aaaccgccta aaggatatat attgagtcaa tcaaccattc tctttgaagt tgaaaaaggc
     3661 agttatgatc cgcttcatcc tataccattg gaaatcatca acgaaccaaa agcaaatccg
     3721 acgaataatg gaaaaacgcc agttcagatg cagacaaatg gtccatcaaa tgaaaaaaat
     3781 ggaggaagct ttccaaaaac aagcgaagag atgcttggag gtttctcaat tttagggtta
     3841 ttgctagtcc tttcaacagg gacagcttgg ttttataaaa aacaacaaaa aggaaacaat
     3901 aggagggaaa taaaatgaaa aacatcaaaa caaaaatatg gacgttttta ggaactgcta
     3961 tcatgctgct tcctttcgtt ctcggcctag gaactgcaga ggtttctgca gccgtatcac
     4021 ctacaccaga aaatgttacg gttaatttgc ataaattgaa attcacttca gcaccagaaa
     4081 atcaaataaa caacggaacg gaattgacat ttccgaatag cgaaccgttg aacggcgttg
     4141 aattcaacgt atatgacatc actgctacgt actatccaag taaagacaca gcagttccag
     4201 cagacgctac acccttcgca agtgtaacga cttctggtga aggattagct aacctgactc
     4261 ttccaggtaa atctgatggg aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg
     4321 gtgtcgaaac gtcgccaaat atcgtgttaa gcttacccta gaccctgaca aacctttaga
     4381 cacgattcac ctatatgcta aaaatgtcat caaagaaagc aatatcaaaa tccagaaagc
     4441 tgggaacttt tcaggggtag ataagttagc aggggcgcaa ttccagatca aaaattcaga
     4501 gaatcaattc gtgaccggtt ttgatgaaaa cactggtgtc gctctctatg atcctgctga
     4561 gtcagcgttg acattcacaa ctgatactga aggatccatc agtgtcaatg gattaataga
     4621 cggcaattat actttagttg aaacaaaagc accagatggt tatcaattaa ccaatccaca
     4681 aaaagaaact acttttatag taaaaaacgg aaaattgcaa caaacaaccg gtatcatcca
     4741 tggcgcagat gaagccaatc ttgggaatcc aaacggtgta gtcaatcggg aaaatacaac
     4801 tacggataac acgattactg tagaaaatgt ccaaaactac ggtgatttcc acttcatcaa
     4861 acaagatgtg aacacgcaag aaaaattagc cgatgcagaa ttttctattc taagagcaaa
     4921 agatccaact gttaaattaa tgaaaaaaat agcaaacctt gcctcaggag aatacccata
     4981 tgcttggtca gatgaagtca caggttctga ctgggaagaa gtgaaattga tttctgattc
     5041 agaagggaaa ttttcaatcg aagggctgaa acaaggggca tactgccttc aagaaacaaa
     5101 agcaccagaa ggctacgcat tacctaagaa tgaagctgcg tacactgaat ttacagtaga
     5161 tgatgatcca acaacttctg atgaaacaac gatcgataac caacctaaag gaatcttgcc
     5221 ttcaactggt ggtatgggga tcatcgtatt catcttggta ggtactgctt tagttggagg
     5281 agcagtgatc tatttcaaaa aacgccatca agaaacagaa gcttaaacta ttggaaacaa
     5341 aaatcatacg ataaataaac gatagtgatt caaatgggga gaaaggttgt gacaagtggt
     5401 cggtctcttg gtattcgctc aacaaacggc aaaataggag gaactatttt gccgtttgct
     5461 tcgctaatac tcaggtcaag gcgctgattt cacaacctct tcatatacat attagaaaat
     5521 aggtggacat gatgaataaa agcagaataa gaatactgga catcttcatg gcaatcattt
     5581 tagtcgttgg gatcgggatc ttttcttatc cttttgtaga agattcactg aatgattttc
     5641 ttgcccagca aatgatcatc cattatcaaa agcaagcatc gaataagaat tcagaggaaa
     5701 taaaaaaaca gcaggaaaaa atgacaaaga aaaaccaaca gttggcagag caaaacgtat
     5761 caccagggat aggcagtttc aaccaagcag ttgatgcaaa agcattaaaa gatttaccta
     5821 gtaatgcctt ttttatggaa catatgcttg gagtcatcga aatcccaaaa atcaatgtca
     5881 gcttacctat attcgatcag acaacggaaa tatttttgca aaaaggaacg tcattattgg
     5941 aaggaagttc ttatccgact ggcgggaaaa acacccatgc cgttctttct gggcatcgtg
     6001 gactcccaga agcaaaatta tttacagatt tgcctaagtt gaaaaaagga gaccagtttt
     6061 tcatccagat caatggaaag acattggcgt atcaagtaga aaaaatccaa gtggtcttac
     6121 ctgacgaagt agattctttg ggtatccaaa aaggcagaga tctcgtgact ttgcttacct
     6181 gcactcctta tatggtcaat acacatcgac tgcttgtgac ggggcatcgt attccttatc
     6241 aagcaaaaga ggcaaaaaaa gcgatccaag gaattgatca atggaaaaaa tggaaatttt
     6301 ttatttggtt tatcggtata ctacttggat caatcggctt agtttggcta ctcatcgctt
     6361 atttagattc tttggccatc gctaagcgaa actatccttt atctttttat gtaaagaaca
     6421 caaacggacg accaatcgaa ggaatggtct tttctgtaaa aacgctcaac ggcaaacact
     6481 atattactag agaaaaagtt ccatttgtga aagcttctga tgaatacgga ctagtgagat
     6541 tttctgattt aaaaggcagg aactatcgct tgcaacatga ggagctcctg cttaaaatcc
     6601 atgtaaaaca taaacattcg aaacagtttt caatgaaatt gaaaaaagga agatataaac
     6661 tgcgaaaaga aaaagaggtg tactatttga ttgagaaaga atgaatgaag atgtccaaaa
     6721 aagcagaggg aacagactaa atgaaaaaag agagaagctg acgaaatgga cgtgtaaact
     6781 gctatttttg gtttactatc tggaacgcca gcttctattt ttttatgttt cagaagaatt
     6841 ttattattat agtataactt ttgaattagt ttccataaca tttggagcaa ggggttaaac
     6901 cacgagcttg agcttcgctt aaagtggcag gggtatacgt accatttcca catttatgtg
     6961 tatggtattt tgaaccggtc ggtgtgacat agacagtttg ttcattattt tgctcaggag
     7021 ctgcggcttg tgcctgctga gaagcctgtt cagctgctgc ttgctgtcgc gcctgttctg
     7081 cttgttgctg tgcgattgct tcttgcctcg cctgttctgc ttgttgctgt gcgattgctt
     7141 cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc ttgctttttc cgttcttctt
     7201 ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag acggcttgtc aactcttgtt
     7261 tgttttcagg tagcgcttgg atagctgctg aagcagttgc atagttttct tttgtaggat
     7321 ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac ttttttttct agtgcagctt
     7381 tttccgcttt ttgtttctct tcttcttttt tcttttcaat ttgttctttc gatgaatctg
     7441 ccgtactttc ggagatcgtg gtactgctgc tggaagcctg agctgaactt gtgctttgag
     7501 tagtagacgt agtagcacca cacccactca acaaacttcc tgcgaccaaa aacaacgcaa
     7561 ctaatttttt cactaaattt cctccttaag taaaaaacaa ccgttttcaa taaatgtcct
     7621 tttcaattgt ttacttactt gttataataa ttgtgttatg aatagtgatt gaggaaactt
     7681 aatctaatcc cgtgttgata cacgggattt tttgctat
//
LOCUS       DACDKL010000117         7675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDKL010000117 DACDKL010000000
VERSION     DACDKL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.117"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IV329_001965"
     CDS             <1..177
                     /locus_tag="IV329_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941134.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
     gene            190..693
                     /locus_tag="IV329_001966"
     CDS             190..693
                     /locus_tag="IV329_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8941135.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="IV329_001967"
     CDS             753..1142
                     /locus_tag="IV329_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8941136.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..3576
                     /locus_tag="IV329_001968"
     CDS             1129..3576
                     /locus_tag="IV329_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8941137.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            3581..5704
                     /locus_tag="IV329_001969"
     CDS             3581..5704
                     /locus_tag="IV329_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8941138.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            5701..6705
                     /locus_tag="IV329_001970"
     CDS             5701..6705
                     /locus_tag="IV329_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8941139.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            6724..7635
                     /locus_tag="IV329_001971"
     CDS             6724..7635
                     /locus_tag="IV329_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8941140.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
BASE COUNT         2440 a         1271 c         1738 g         2226 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tggacaagct gtgattgtcg atccaaaagg ggaacggggt
     2641 ggctggaaag aaaccttgcc agaaattgcg gatgaaatca atattctcaa cttaactagt
     2701 aagccaaaaa atcaaggatt acttgatcct tatgtgatta tggagaaaaa gaaggattct
     2761 gaaagtctag caattgatat tctgaccttt ttaacaggaa tttctagtcg agatggggag
     2821 aagtttccag tgttgcgtaa ggccattcgg acggttaccc aaagcaatca acggggatta
     2881 ttacaagtaa ttaatgagct tcgaaaagaa ggctcaccag ttgcggaaaa tatcgcggat
     2941 cacatcgagt cctttaccga ttatgatttt gcccatttgc ttttctcaga tggttcgatt
     3001 acccagtcga ttagtttaga taggcagtta aatatcattc aagtagcgga tttggtttta
     3061 ccagatgcag aaaccacttt tgaagaatat accaccatgg aattgcttag tgtttccatg
     3121 ctaatcgtca tttccacttt tgccttggac tttatccata gtaatcgaga aatcttcaaa
     3181 attgtagact tagatgaggc gtggagtttt cttcaagtag cgcaagggaa aacattatcg
     3241 aataagctcg ttcgtgctgg gcgggctatg aatgccgggg tttactttgt cacgcaaaat
     3301 agtgatgatt tactggatga acgcttgaag aataatatcg ggttgaagtt tgcgtttcgc
     3361 agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggcttgga tcgagaagat
     3421 gaaagcaatc aaaaacgcct acgagatttg gaaaatggcc aatgcttgat gcaggatttg
     3481 tatggtcggg taggcgtggt tcaggtacat ccagtctttg aagaactctt ccatgccttt
     3541 gatacccgtc cacctgttca agagagaatg gagtgagggg atgaaaaaga aactttttcg
     3601 gattgtggga atcgttttta ttagcatact cactctgata gttctattga gtttagttgg
     3661 tacggtagcc gaggcgacag ggctagttga tgatacagtt gaagcaggaa atctatattc
     3721 caaatattca ctgaacaact atcaactaga tttctttgtg gatagctctt gggattggtt
     3781 gccttggaat tggggtgatg gcttagggaa aagcgtgatg tatggtcttt atgccattac
     3841 taatttcatt tggacggtga gtttgtattt gtcgaacgcc acgggttatg tggttcaaga
     3901 ggcttacaaa ttagatttca tttcggatac ggctgaaagt attggaaaga atatccaaac
     3961 attggctggt attactgaaa atggactcca gacctccgga ttttattttg ggtttctatt
     4021 attgatgatt ttagctctgg gggtgtatgt ggcgtatacc ggattattaa aacgagaaac
     4081 cacgaaagca gttcgagccg tgattaattt tgtgatgatt tttctactat cgggttcgtt
     4141 cattgcttat gcgcctacct atatcacaaa aatcaatgac tttagctcag atgttagtga
     4201 agcggccctt acccttggaa ccgagatcgt tgtacccaat tcagaaagcc aaggaaaaga
     4261 tagtgtggat ttgattcggg atagtttgtt ttcgattcaa gtccaacaac cttggttact
     4321 actgcaattt gatgattcca acatagaaga aattggtgaa gaccgggtca acaagattct
     4381 atctgtgagt ccagatgaaa acaaaggcaa agatcgggaa gaagcggtca aggcagagat
     4441 tgaagacaat gacaatgcga atctcagtat taccaagacc atgaaccgtt tagggaccgt
     4501 ggtctttttg gtattgttca atattggcat ttccttcttt gtttttcttc taacagggat
     4561 tatgttgttc tcgcaaattc tcttcattat ctttgcgatg tttctgccaa ttagtttctt
     4621 attgtccatg ttgccgactt atgaaagttt aggaaagaag gcgattattc gtttattcaa
     4681 tacgattatg atgcgagcgg gtgttacttt ggtgattacg actgcattta gcatctccac
     4741 gatgtttttc aatatttcgg caacctatcc atttttcatg gttgcttttc tgcaaattgt
     4801 gacctttgcc ggtatctatt tcaaattggg cgacattatg agtatgttca acctccagag
     4861 taatgatagc cagtcaatgg gaagacgcgt catgcgaaaa ccgcaaatgt taatgaatcg
     4921 aaaattgcgg caactcaatc gaaatgtggg acgagctttg gcttttggtg gtggtgcagc
     4981 agtcggaaat aagttggcaa aggaacaatc aaaatccaaa tttaagccat ccggttcttc
     5041 tctacgaaag aattcacgat taccgaacga tcacgaaata ctatctgact taactaagga
     5101 gaatccagtt tcaaataata agaaacaatc ccgaatgaat ttggctggga gaaaaatcgg
     5161 caaagttttg gatacccaag ctttggtgaa agataagact aaacaagtaa aggatcaggt
     5221 aaggaatacg ccaacgaatt tgaaatacaa tctccataaa ggaatcgaaa agtcgaaaaa
     5281 agcaccagaa gaattcaagc gcggactgtt ccaagaaaaa gcggatcgag tggatttgcg
     5341 agaaaaacaa cgacaacgaa gaaatgcgaa aatggacgaa aaacgcaaga tactgagtga
     5401 agcaacaaat cgtcagggaa aaagaagggc gaatattcca atagataaag cagctcaacc
     5461 tcagaaagat atgatgctga aaaaagaagc accaaagcgg attgtgagag caaatctgaa
     5521 tcccgaaatc aaacgaaaac taactagtca agaaatcatt tcaaaaggga aaagccaatc
     5581 agcaagtaag aaaaattctt tccaacaagt gaaacagagg tccactctgc caaagcagac
     5641 caatcaaaaa gtacaaaaaa gaatgaacca tcgtccaaaa tcaaggtcag gtgagaaaaa
     5701 atgaacttga agaaagtggg tgttatttca ttaacttttc tgaccatctc ttttatgggt
     5761 attttgatgt gtgtgggttt gttgtttggt gaagacagtg agggaaatgg tagttctggc
     5821 acttctcctg gcggaactag tgtctcagaa gaagtattga aacaccgtgc aatggtggaa
     5881 aagtatgcca aagaatcgga tataacagag tatattccaa ttcttttggc gattattcaa
     5941 gtggaatctg gtggcacaat ggaagacgtt atgcaaagtt ctgagtcagc cggattacca
     6001 cctaattcat taagtaccga ggcatccatc aaacaaggct gtctctattt tgcgtctttg
     6061 gttaagcgtt ccaaagaact agggtgtgat caagatagta ttattcaggc ctataactat
     6121 ggtggcgggt atctagatca tgtcgggaaa aacgggaaga agcatagttt tgctttagct
     6181 gaatcttttt caaaggaaaa atcaggtggt caaaaagtcg attaccccaa cccaatcgcg
     6241 attaaggaaa atggtgggtg gcggtataat tatggcaata tgttttactg tttattagtg
     6301 aagcaatacc taactacaac tcagtttgat gacaaaatag tccaagaaat ttttgatgag
     6361 gcatttaagt atgaaggaac ggcctatgtg tttggtggct caagtccaga aacggggttt
     6421 gattgtagtg gcttgaccca atggtgttat gccaaagttg acctgaaact tcctcgggtg
     6481 gctcaggatc aatatgatgc catgactcat attgatctaa aagatgccaa accaggtgat
     6541 ttagttttct ttcattcaac ctatgatgcc ggtacttatg tgacccatgt gggcatttac
     6601 gcaggggaga atcgcatgtt tcatgcagga gatccagtgg ggtggacgaa tctaacggaa
     6661 aactattggc ggcagcatct tatcggagct ggccgataca aataagagaa aagaggaaaa
     6721 agaatgaaga taaaaattga acggaatcaa aaggaaaaga gcccaaaaaa gaaaaaagaa
     6781 cgagttgtat ccgtgggtaa gcatcgaaaa atggtgcttg ccctttggct tttattgtct
     6841 tgtagtcttg cttttggcgt ttataaaaat ttcaccgcca ttgatcaaca tacaactcat
     6901 gaaaaagtgg ttataaaaga gaaggtagtg aatacttctg gcgttgaaag ttttacagag
     6961 gattttgcga aagaatattt ttcatggaaa aataacaaag aagcaattga aaagagaatg
     7021 agtaatctcg aacagtacct tacagaaaac ggtctcgctt taatccaaga tatggttcga
     7081 gcagatattc ccacgagttc cgaagttcaa tctgtaaaaa ttctagatgt tgaaaaacac
     7141 tcggaagaat ttgtcgtttc atttttagtg gagcaacata ttacagaggg aaaaaagacg
     7201 caatcaattt cttctgcata tcgagtgaca atctttgaag atgaaaacag gaatcattta
     7261 gtaactagtt tgccaacaat gattggaaag ccagacaagg caaaatatga agcaaaacaa
     7321 gtagaaaacg attcagaaat tgatgccaaa acaacggaag aaatcaccga ttttttagaa
     7381 acatttttta aactttatcc atcagcttct gaaaaagaat tggagtatta tgttgaaaat
     7441 ggtgcgatgc gaccaatcaa tattgactta aagttttcgg agttagagaa tccagtttat
     7501 ctacaaaacg gaaaaaattt tctagtgagt acaaagataa aataccttga taatgtatca
     7561 aaaaccacta gctactttca gtacaaatta gtattacaaa aaaatggaaa ctggaaaatc
     7621 gttgatgtta aataataatg actcaatcta attttattag aagttataag tagtg
//
LOCUS       DACDKL010000118         7652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDKL010000118 DACDKL010000000
VERSION     DACDKL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.118"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(161..838)
                     /gene="nth"
                     /locus_tag="IV329_001972"
     CDS             complement(161..838)
                     /gene="nth"
                     /locus_tag="IV329_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8941141.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(845..1543)
                     /locus_tag="IV329_001973"
     CDS             complement(845..1543)
                     /locus_tag="IV329_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8941142.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1811..2995
                     /locus_tag="IV329_001974"
     CDS             1811..2995
                     /locus_tag="IV329_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8941143.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3001..3444)
                     /locus_tag="IV329_001975"
     CDS             complement(3001..3444)
                     /locus_tag="IV329_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8941144.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3670..3957)
                     /locus_tag="IV329_001976"
     CDS             complement(3670..3957)
                     /locus_tag="IV329_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941145.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4128..5480)
                     /locus_tag="IV329_001977"
     CDS             complement(4128..5480)
                     /locus_tag="IV329_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8941146.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5753..6199)
                     /locus_tag="IV329_001978"
     CDS             complement(5753..6199)
                     /locus_tag="IV329_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8941147.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6435..7361)
                     /gene="cysK"
                     /locus_tag="IV329_001979"
     CDS             complement(6435..7361)
                     /gene="cysK"
                     /locus_tag="IV329_001979"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8941148.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2080 a         1562 c         1256 g         2754 t
ORIGIN      
        1 ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt
       61 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
      121 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      181 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      241 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      301 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      361 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      421 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      481 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
      541 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
      601 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
      661 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
      721 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
      781 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
      841 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
      901 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
      961 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
     1021 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1081 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1141 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1201 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1261 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1321 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1381 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1441 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1501 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     1561 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     1621 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     1681 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     1741 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     1801 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     1861 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     1921 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     1981 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     2041 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2101 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2161 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2221 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2281 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2341 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2401 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2461 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     2521 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     2581 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     2641 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     2701 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     2761 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     2821 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     2881 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     2941 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     3001 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3061 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3121 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3181 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3241 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3301 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3361 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3421 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3481 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga
     3541 aaaaaagggg ttgacaaagc ttgtcataac ccctttttct gattaaaaga tagtaaagag
     3601 acagtcatcg gtaattgtga tgattttttg tcatcactta aggatgagcg gccttttcaa
     3661 aaactgtctt taaagatcat catcagtttc tagcacatat ggctgacctt catatgtgta
     3721 ctctgtattc aatgtgccgt cattttcatc tgagtagatg cgtctactga aaggaaaacg
     3781 atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg taatattcct cttgcgtttc
     3841 attcaactga gagagtactt gatctgccaa ctcatcaata ttgatcgtta atttcttcga
     3901 agcactttta taatcttcta aaaatgcatc ttgttggaca aaatattctt tattcatctt
     3961 ctttctcacc tttcttttca aaatcaaata aagctgcttg ttcttttatg taacagcttt
     4021 atctttgctg cttattgtga caaaaaattc gaagaaagac aactaattgt acttttcaca
     4081 aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt
     4141 tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa
     4201 tgtggttgga agaagaaatc tgaaagtgcg agatcttcta cagtcatttt attttgaaca
     4261 gctaaagaca tggtatttgc tgattgtgta atgtcgtatt tggacatgaa ctgagcacct
     4321 acgatacgat tcgttccttt ttcatagact agctccatca ttactttttc agtagttggc
     4381 atgaactctg gacgataatt atcttcaaat acagtcgctt ggacttctag atcgttcaaa
     4441 gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct
     4501 gaagttcctt gagtaccacg atacgccatt ttatgatctg tcaagttatt tcccacaagc
     4561 aaaccttgac gaacggcatt tgttgccaaa ggaatatagt tttgcgtgcc acttggattg
     4621 tagttgacta ctgcagagtc tccagctgca aaaatgtctg gattacttgt ctgcatgtat
     4681 tcattcactt tgattgcccc gtttggcagt gtatctactt ttccttcgat cagttttgta
     4741 tttggacgga agcccacaca taaaatgacc atatctgtat caaatgtatc tgttggtgta
     4801 gctacttgtt tcacttttcc ttcttcatct gtgataaatt cagtaacatt ttcgcctagc
     4861 gcaagagtaa caccgcggtc tgtcaattct ttttctaaga tatctgtgaa tggtttatct
     4921 agatatttat tcaagatgcg atctaatcca tcaatcaatg tcacttgttt tccagattcg
     4981 acaaaagctt ctactaattc aataccgata tatccaccac caacaattac tacacgtttt
     5041 gcttctttcg cttgtgcaat aatttcattt gcttgattgt agtttttgca taggagtaca
     5101 tttttcgcat cgattccact aatcggtggg ataataggcc atgaaccagt tgtcatcact
     5161 aatttatcat aagaaaccgt ttctgtttct cctgttttca gatcttttgc cgttactgtt
     5221 ttattttctg tatcgacgtc tgttacatcg tgttccattt taactgttgc tcctaatgaa
     5281 gataattctt caggatttga gtagaacaag cctgctgggt cctttactac tccgccgaca
     5341 taaagtgcga taccgcaaga taagaaagaa atattgtcat ttctttcata tactgtcact
     5401 tctgcttctg gatgattttt taaaatactt tttacagctg cagtacctgc atgagtacag
     5461 ccaattacta ctactttcat ttatggtccc tccgattaac attttgtcct tcgacaaaac
     5521 tactataaca gaaataaaac ggatacaaca gtttaatgct tgtgaattca caaaggtttg
     5581 tttgtagttt cattcctttt caagcataag aacccaatat ctacaagatt cttattttaa
     5641 taatattcac ttttatatct aagattgtga ataaaagaca aaaaaataaa aaagctagcg
     5701 ttttttttac acagctagct ttttttaggt ataacacttg tagaaactgt ttttacattt
     5761 gcgccttttt tgctgactct ttacaatccg gacagagtcc atacaattcc aaccgatgtt
     5821 cattaatctc aaaatcagtc agtctgcttg cagccatttc tatatcttca agccctgggt
     5881 aatgaaaatc aacaatctta ccgcagtttt cacaaattac atgataatga cgctttgaac
     5941 tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc ttgaacaaat ccaatcgaag
     6001 taaataagcg aaggttattg taaacagtcg caacactcat atttggaaac cgatgttcaa
     6061 gtgctttata aatctcatca gccgttggat gggaatgatg ttcaatcaaa tattctaaaa
     6121 ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt taacttttcg ataacctctt
     6181 cgactaactt attttccatg taaacacccc acgttgttat tctcgtctta tcatactatt
     6241 tttacatttt aaaatccact taacatgaaa agaaaattca attttattgt attttaatga
     6301 aaaaagacgt aaaacagtcg cgttcactat tctacgtctc tattcaaata atttttctca
     6361 tcataaattt ttgtgtgttc ttttcatctt taaaagtttt ttacataaac ctatctggag
     6421 aaaaaccaag tgccttatat ctcttggtaa agggctgtag ataaataacg ttcaccattg
     6481 tcgggaacca cagctaatac cttcttgcct tttcctaatt cttttgcgac ttttagcgcg
     6541 gcagcaattg cagcgccaga tgagatgcct actaaaatcc cttcttttcg cccaacttct
     6601 ctagctgttt ccatcgcttc gtctccacta atagaaagga ctttatcata cacagaagta
     6661 tccagtgttt taggaacaaa acctgtaccg attccttgga ttttgtgagg tcccgcttct
     6721 ttcccttcta aaatagcaga ttctgctggt tctaccccga ttaattcgat tttcggatac
     6781 acacgtttta attctcggcc agcgccagtg atcgttcctc ctgtaccgat acctgcaaca
     6841 aacgcgtcca agccatttac tccaaatgct tgatgtatct caggaccagt cgtcttctca
     6901 tgcacaagag gatttgcttc attttcaaat tgtaatggta aaaagtagcc gttttctttt
     6961 gccaaacgtt ctgcttctct gattgatcca gaaataccat ccgcaccagg agtcaaaatc
     7021 aactctgcgc cgtacccttt catcaataaa cgtctttcga tactcatcgt ctcaggcatc
     7081 acgatgatta ctttatatcc ttttgcaact cctaccatcg acaatccgat tcctgtattt
     7141 ccagaagtag gttcaatgat agtatcacct ggtttcagta gaccatcgtg ttctgctttt
     7201 tctatcatac ttaaagcaat acgatctttt acacttcctc ctggattaaa aaactcaagc
     7261 tttacaaaaa catctgctga atcttctggt acgattttat ttaatttgac gattggcgtt
     7321 ttgccaatca attcggtgac tgagttatag atagctgtca tcttcaattc ctccttgtct
     7381 ttgctcttct tacttatcat aatacaatct gttcatttat ggcgagtgat tcgtctttga
     7441 ctagcaaaaa aaagacaaag atataccata tatggtaatt tcggctctat aatttatggg
     7501 actgatgaat cagaattgat tcttcagccc catttttatt ttaggtatta aaaaacatat
     7561 cgttctctag tatgattaaa tcacgacaaa ataaacaact gagaggacga tatgctatat
     7621 gaatgattct atcaaaaaaa tgctgcgatt aa
//
LOCUS       DACDKL010000119         7513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDKL010000119 DACDKL010000000
VERSION     DACDKL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.119"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            238..711
                     /locus_tag="IV329_001980"
     CDS             238..711
                     /locus_tag="IV329_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8941149.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            708..2840
                     /gene="feoB"
                     /locus_tag="IV329_001981"
     CDS             708..2840
                     /gene="feoB"
                     /locus_tag="IV329_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8941150.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2842..2976
                     /locus_tag="IV329_001982"
     CDS             2842..2976
                     /locus_tag="IV329_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8941151.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3070..3714
                     /locus_tag="IV329_001983"
     CDS             3070..3714
                     /locus_tag="IV329_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8941152.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3901..5094
                     /locus_tag="IV329_001984"
     CDS             3901..5094
                     /locus_tag="IV329_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8941153.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5087..6814
                     /locus_tag="IV329_001985"
     CDS             5087..6814
                     /locus_tag="IV329_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8941154.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7208..7405
                     /locus_tag="IV329_001986"
     CDS             7208..7405
                     /locus_tag="IV329_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8941155.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7407..>7513
                     /locus_tag="IV329_001987"
     CDS             7407..>7513
                     /locus_tag="IV329_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8941156.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVY"
BASE COUNT         2455 a         1281 c         1653 g         2124 t
ORIGIN      
        1 aatagcaaat ttaatagaga aaaaattgtg gaaaactatg aagaaatatt tttttctcat
       61 ttttatctat caatactaaa gattcgctca taatatcaca agtataattg acattctcaa
      121 ttaccgagag tattctatgt gagaatgatt ctcatttatt tgcgtaacac gtaagaaaat
      181 tagaacatat aatgaatgat tttaatacat gatttatttc acaagagagg aatcaatatg
      241 cagaccttag cgcaggcaaa gcagaatttc gtttatcaag tcgaacgaat cgaaacagaa
      301 gaaaaaacaa aacgccattt gcaaaatatc gggatcgttg ccggaagtaa aatcgtcgtc
      361 gtcaatttat caggcaacaa tgccatttta ctggtaaaaa gtcatcgagt ggcagtaagt
      421 cgggaaatac taggtcaaat ctttgtcaat aaaaagactg cttcctctga aagctggact
      481 tctcttgatc aattaaaagc aggagagact gggatcgtcg tagcaatcca cggacaaggc
      541 gcagtgaagc gacggttaat ggatatggga ttgacgcgaa atgtaaaaat agttatccga
      601 aaactagccc ctctgggaga tccaatcgaa ctgacagtgc gagggtatga actgacgtta
      661 cgaaagtcag aggccgaact aatcttgata caaagggagg aaaaagaatg aatatcgcgt
      721 tagtcggaaa tccaaacagc gggaaaacta gcttgttcaa tctcttgact ggttcgaatc
      781 aatatgtagg gaattggccg ggcgtaacag tagaaaaaaa agaaggatcg ttgaaaaaaa
      841 atccgaacat tcatattcag gacctacccg gcatctattc tttgtctcct tatacaccag
      901 aagaagtgat tgcaagagat tatttggttc aggaacagcc tgaactgatc atcaacatta
      961 ttgatgctac caatttagaa cgaaatcttt atttaactac gcagttattg gatttagaca
     1021 ttcctatgat cattggtctg aatatgatgg atgtcatcaa aaaagaagga aaaaaaatca
     1081 atctagaaaa gctttcctat ggtcttggtg taccagtttt accaatcagc gtattgaaaa
     1141 agcaaggaat cgaaaaacta atcgaaaaga ttcaggagtc tgcaaaaaag cggccttcta
     1201 ttcctgttcc ctgtacatat gatccacgtt tagaagcagc tttacatgaa attgaagatg
     1261 tactaggaga acaaagcggc aagaaacgct ggtatgcaat gaaatatttt gaacgagatc
     1321 agaaggtaag agaggattac gagatagcag tttctcagca aaaagagatc gagcaactga
     1381 tccagttaac agagaaacta ttggacgacg atagtgagac gattctggtc aatgaacgtt
     1441 atgaatttat tacccaacta tgtacactct gtgttgtgtc aaatgattct ttccaattat
     1501 cgatgagtga caaaatcgat cagattgcaa cgaatcgttg gttagccttg ccgatcttcg
     1561 catttgtgat gtggctgatc tattatctgg ctattcaaac agtaggaaca atggggacag
     1621 attggatcaa tgacacattg tttggtacat ggttaccaga acatgttggc agattgttag
     1681 ctgaatggca agtagcggga tggatgcagg agctgatctt gaacggcatc atcgcaggcg
     1741 tgggagcagt gttaggattt ctaccgcaat tgattatatt gtttttatgc ttgagctttc
     1801 tggaagactg cggatatatg gcgaggatcg cttttgtcat ggatcgactt ttcaggaaat
     1861 tcggtctttc aggaaaatcc ttcattccta tgctgattgc tacaggatgt ggagtgccag
     1921 gcgtcatggc cagtcgcacg attgaaaacg agcaagatcg tcgtttgacg atcatggtca
     1981 caaccttcat gccttgttct gcaaagttgc cgattattgc cttagtagca ggggcctttt
     2041 ttcctcatca atcttgggta gctccttctg cctattttct tggtatgact gcaattattt
     2101 tctctggaat cagcttgaaa aaaacacgtt tgtttgctgg agatacagct ccttttatca
     2161 tggaattgcc cgcatatcat tttcctcaat ggtcagcggt cctaagacaa acctatgacc
     2221 gcagcaagtc ttttgttaaa aaagcaggaa cgattatctt tgtttctagt atcgtgatct
     2281 ggttcagctc ttcttataat ttttatgctc aacctgtttc tgaagaccaa agtatcttag
     2341 cctttttcgg gcgaatcctg gcagttgttt ttcagccgct tggttgggga aactggcaag
     2401 gaacagtcgc agcgattaca ggattggtcg ctaaagaaaa tattattggc acatttggta
     2461 ttttgtttgg tcatgcggaa gtatccgaaa atggcagaga aatctggcaa gtgctacaac
     2521 acacatatac tcccgcagca gcttattcat tgttagcctt caatttactt tgtgcgcctt
     2581 gttttgcggc aattggggct atccatcggg aaatgaatgc caagaaatgg acttggatcg
     2641 ccataggcta tcaatgcggt ttagcttatt tagttagttt tgtgatttat cagttgggac
     2701 acttattgga gggtggacag gtagcgttag ggacgtatct tgctatttta ctgatgatag
     2761 gactcgttta tgttttgttc cgtcagccaa aatccaagaa ccagttttat actatattgt
     2821 cattagaagg agaggaatag gatggcgaca ttcttattaa gtgtgttaat ctttggaagt
     2881 gcaggcgcaa ttgtctatac tcgtttaaaa tccggtaaga attgtgatga ctgtcaaaca
     2941 gcttgcccag taaaaaagga acagatcaag cgatgatcag tcgttgcaag aggctattct
     3001 cccctttttt gcttattttg ctagagcata gtaagatgag ggaaagtaaa aaaagaaaga
     3061 ggaaagaaaa tgtctggaaa gaaaaaaatc gtccgcccgc gctaccagca gattgctgtt
     3121 gatttagccg aacgtatcgt tgaaaaccga tataaagtgg gtgagaagat ccatgcccgc
     3181 tcaacacttg ccagtacgta caatgcttct cctgagacta cccgcaaggc tatcaatgtg
     3241 ctggtagatc ttggaatcat ggaagtacgg catggaagtg gggcatttgt tgcttcgaag
     3301 gaaaaagcaa aagatttcct attgcaatat caagatgtac aatccatcca agatacaaaa
     3361 gcagaaatca tggcaagtgt ggagaagcaa caagatgagc tgaatcattt ttctcaatta
     3421 ttggatcagc tggtcaatca aacgaagcga atacataaga tgaatccttt acttccgttt
     3481 gaattatctt tatcagaaaa agcaatgcat ctggaccgtt caattagcga gttgaatatc
     3541 tggcaggcga ctggcgccac agttatcgca atttctcata atgaagaact gcttgtttca
     3601 cctggcccct atgctaaatt tacggcaggg gacatcgttc attttattgg aaatgaatat
     3661 acactacagc gtatgacaaa ctttttttac ccggagcatc agcttgcaga atgattgtgt
     3721 gaaatattaa aaaaatatta acttggctaa accttttatt tacaaagatt tagccaagtt
     3781 tttttcagtt tggacaagca aatttctttc atttttgaca aagtgactac atgatgttaa
     3841 catagtgtgg tcactttttt cagaaagaaa aatgaattta cataaaaaag gagaatttgt
     3901 ttggtaaaag tagaagtaaa acatttaaca aagatatttg gtaaaaagac acaagccgct
     3961 ttagatatga tgaatgatca ccagccgaaa acggagattt taaagaaaac cggtgcaacg
     4021 gttggtgtgt atgacgtgaa ttttgacgta aaagaaggag aaatcttcgt gatcatgggg
     4081 ctttccggga gtgggaaatc cacattgatc cgtctattga atcgtctgat tgagccaact
     4141 tcgggcagta tctatattga tggagaagat gtatcgaagt taagcaaaga agaattgcga
     4201 gaagtccgtc gtcataaaat caatatggtt ttccaaaact tcggtctgtt tccacatcga
     4261 acgatcttgg aaaatacaga atacgggtta gaagtccgtg gtgtacctaa agaagaacga
     4321 caagaaaaag cagaaaaagc attagaaaat tctagtttgc tttcatttaa agaccaatat
     4381 cctagtcagt tatctggtgg gatgcaacaa cgggttggcc tagccagagc tttagcaaat
     4441 gatcctgaga tcttgctgat ggacgaagcg ttctcggctc ttgatccgct gatccggcga
     4501 gaaatgcaag acgaattact ggacctgcaa gccaatgttc aaaaaacgat tattttcatt
     4561 acccatgatt tgaatgaagc tttacggatt ggggacagaa tcgccttgat gaaagacggc
     4621 gaaatcatgc agatcggaac aggtgaagaa atattgacca atccagcaaa tgattatgta
     4681 cgtgaatttg tggaagaagt cgatcgttcc aaagtgctta cggctcaaaa catcatggta
     4741 cctgcattga cgacgaatat cgaatctgat ggaccaaacg ttgcattgac aaggatgcgt
     4801 aatgaagaag tcagcatgct gatggctgtt gacagaaaga gacacctgaa agggatcatc
     4861 actgcagatc aagcgttaga agctaggaag caaaaacgtc cattgatcga cttcctagat
     4921 gaaaatgtga cggtgattgg gaaagacatg gtggtaagtg atattttcaa tattatttat
     4981 gactcaccga ctccacttgc agttgtagag gatggaaaat taaaaggtgt cgtcatccga
     5041 ggtagtgtga ttgaagcatt agcagaaaca agcgaggtga gtgaacatga ataatctatt
     5101 gaactatcaa ttaccagtgg caaactgggt agaaaatatc acagaatggt ttacgacaac
     5161 tttttctggc ctattttcat ttttacaaac gattggacag gcagtgatgt cggggattac
     5221 aaatttactg ttagtgattc cagctccttt gtttatcttg ttattgacgg tagcagcttt
     5281 ctttatttct aagaaacgtc caggacttac gttgttcaca ttaatcggtc tatggtttat
     5341 ttataaccaa ggtctatgga atgatttgat gaatactgtc acacttgttc ttttatctag
     5401 tgtgatctcg atcatcattg gggtaccatt agggatcttg atggcaaaaa gcagtaaagc
     5461 ccaaagtatt atcaaaccga tccttgactt tatgcagaca atgccaggat tcgtttattt
     5521 gattccagca gttgcatttt tcggtatcgg gatggttcca ggggtatttg cctcagtcat
     5581 ctttgctctg ccaccaaccg ttcgttttac gaatttaggg attcgacaag ttcctaaaga
     5641 attagtagaa gcttctgatt cattcgggag cacaagtcgt caaaaactat tcaaactaga
     5701 attgccttta gctaaaagta cgatcatggc agggatcaat caaacaacaa tgctgtcact
     5761 ttcaatggtc gttattgcct ctatgatcgg tgcacccggc ttaggacgcg gcgtattatc
     5821 agcattgcaa agagcacaag tcgggaatgg atttgtcaac ggggttgcat tagttatctt
     5881 agcaatcatc attgaccgtt ttacacagca tttgaaccag ccaaacaata aaaaagctgc
     5941 aggagcaaca acacagaaaa gtaaaaaacg tcaaggactg atcattggtg ctgtagtagt
     6001 agtgatctta ggtgcgattg gtatagggag tttttcatct gccaaagaaa caaaacgtat
     6061 caacttatct tatgttgaat gggatacaga agttgcttca acaaatgtag tcggtgaagt
     6121 attgaaacaa atgggctatg atgtaacgat gacgccatta gataattcaa tcatgtggaa
     6181 atctgtttca aatggcgagt cagatgccat ggtttcagct tggctaccta aaactcatgg
     6241 ttcacaatat gcacaatata aagatcaagt cgaagaccta ggtgccaatt tgacaggagc
     6301 aaaagttggt ttagcagtgc cagcctacat ggatgtaaat tcgatcgatg agttaaccga
     6361 tcaggcaggt aagaaaatca taggaatcga gccaggagct ggtgtagtta cggctgctga
     6421 aaacacgatc caaaaatatg acaacttgaa agactggaaa gtagagacat cttcttcagg
     6481 agcaatgacc gttgcactag gtcaagcgat caaaaaacac gaaccaattg tagttacagg
     6541 atggacacct cattggatgt ttgccaaata tgatctgaaa tacttagaag atcctgaaaa
     6601 cggaatggga agcgaagagc agatccatac gatggttcga aaaggattga aagaagacca
     6661 gccagaagct tacaaagtat tagacaattt ccattggtca gaaaaagata tggaaaaagt
     6721 tatgttggaa atcaacaatg gaaaagatcc acagcaagca gcaaaagact ggatcaaaga
     6781 gaaccaagaa ttagtcgaaa gctggaaaaa ataaaaagac agacacgaaa taaatggagc
     6841 ttccaaaatt ggatcaaata tccaaatttg gaagctccat gttcgtctct atcttttttg
     6901 tctataaaaa aagagaatgc gtagctcatc ttcgaaacgt ccaaagacgc cgacaaacta
     6961 ttatgaattt atgggtgcga gtcaaagaga tcgatcgtag gtatgaaaga ctaatgtgat
     7021 taaagtttat tatgtacgaa gtgcattctc aaaaaatatt aaagaatata catagtttat
     7081 atacttaaaa gtgattaaat tcttattcag gaattatttt gctacgtttc aagaaggaat
     7141 tgttttttga aatttaatga atgttagaat aatagtctaa aaacattaat ttaggggtga
     7201 ttagattatg aaacatttaa aaattttgtc tattaaagag acacaactta tctatggggg
     7261 taccactcat agtggaaaat attatggaaa tggagtgtat tgcactaaaa ataaatgtac
     7321 ggtcgattgg gccaaggcaa ctacttgtat tgcaggaatg tctataggtg gttttttagg
     7381 tggagcaatt ccagggaagt gctaaaatga aaaaaaatgc taagcaaatt gttcatgaat
     7441 tatataatga tatatctata agtaaagatc ctaaatattc tgatattctt gaggttttac
     7501 aaaaggtata ttt
//
LOCUS       DACDKL010000120         7345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDKL010000120 DACDKL010000000
VERSION     DACDKL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.120"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..1961
                     /locus_tag="IV329_001988"
     CDS             126..1961
                     /locus_tag="IV329_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8941157.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2021..2737)
                     /locus_tag="IV329_001989"
     CDS             complement(2021..2737)
                     /locus_tag="IV329_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8941158.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2964..3506)
                     /locus_tag="IV329_001990"
     CDS             complement(2964..3506)
                     /locus_tag="IV329_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8941159.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3511..3738)
                     /locus_tag="IV329_001991"
     CDS             complement(3511..3738)
                     /locus_tag="IV329_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8941160.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3817..5697)
                     /locus_tag="IV329_001992"
     CDS             complement(3817..5697)
                     /locus_tag="IV329_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8941161.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5886..6500)
                     /locus_tag="IV329_001993"
     CDS             complement(5886..6500)
                     /locus_tag="IV329_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941162.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6642..7202
                     /locus_tag="IV329_001994"
     CDS             6642..7202
                     /locus_tag="IV329_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8941163.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2244 a         1469 c         1216 g         2416 t
ORIGIN      
        1 tcatcgaata cttcgctttt gattcgattg aacaattaac tcagcgttgg aaaaaaagaa
       61 ttcattggct ttttgttcat aaaagttatt ttttagttgc agcatttttt tactgcttta
      121 ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg gaaagaaact atccaggttg
      181 ccttaaaaat atttactcag cgacaagcac ccatgatcct gacaatcatc attattatgg
      241 gatttttctt cttactattg ttgatttcca atcgtttttg gtacgccttg actgctacct
      301 tgatcattaa cttactgctc actatttcga cagtaatcaa aatggaaatg cgggaagagc
      361 ctgtctttcc ttcagattta aaaatgctga ccggattgtc agaactatta tcgatggtta
      421 gcccagtctt attaatcgtg ggcggattga tccttctcct attattgatt actagtatta
      481 ttgtacaacg tagactacaa aaacagtatt ccttgaagat tcattggaaa cgtcgattta
      541 tcagtatcgc tgtattatta ggatgttttt cgggtgtctt tttcattaat cacaaaaatt
      601 cgccttcttt cttgctgttt aatttattta aagtaaacaa gactttcttt aaccagcaag
      661 gcgccgtaaa agataacgga ccagtcattc aattcctaaa caatatcgac attaaaatca
      721 tggaagaacc tgctggctat tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca
      781 caaaggcaga tgaaatcaac caaacaagaa gtgactggtt aaacaatcag acgatcgttc
      841 tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc taatctgaca gtacctacag
      901 accctattcc ttacatcaaa agcattataa cagaaggaac aggaggattg atgttatccg
      961 tcggttatgg tggcggaaca gctaacatgg aatgggaagg actgacaggc ttgagtatca
     1021 gtaacctgtc tgcttcgtta gttacacctt atacccagct agtggagcgt cagaaaataa
     1081 gtccaaatat taccgatcta ttcgatgaaa aaatagccat ccatcctttt acagctgctc
     1141 tttatcgcag aaaacaagtg tttgataaat ttggttttga caggttctat tatttagaca
     1201 gtccggataa attaacttat acggataaaa tagaacaaag cccacgcata tcggatgatt
     1261 cagcatacaa agaaaccttg aaagctttac aaagcaatac ggaaacgtca caattcatcc
     1321 aattatccac tatgcaaaac catatgcctt atgatgatta ctatagtgaa ttgaactaca
     1381 ctgctgaagg aactgctgtc attcctaata gaagaaaaga attacaaact tatatgcaag
     1441 gccttcacta taccgataca gcagtaaaaa aattcatcca agaaatcgac aaaatcgata
     1501 aaccgattac ctttgttttt tatggtgacc atttacccgc aatctatagt gggaatgatt
     1561 cgaaaaaata tggattagaa cagcaccaga cagattattt catttataac aacaagtata
     1621 gtcgtgaaca agcagctacg ttaaataaag aagttgttgc tcctaataac tttggtgcta
     1681 tggctcttga gcatgcgaat atcaaagtaa ctccttacta tgcgttgttg acagaagtat
     1741 ctaatcatct acctgcatta acaattgatc ctacagaaag cttgtctaat cgttttaatg
     1801 gaaaacaggt ctttgttaca gaggatagtc aaattatcca agaaaaaaat ttaactgaga
     1861 aacaaaaaga aattctagaa gattatcgtc tgatacaata tgatcttgtt gcaggtaacc
     1921 agtacagtgc aacatgggcg acacaaaaaa taaaagacta acaaataaac tgagcaaggg
     1981 gttgtgataa aagacatgtc acaacccctt gtttatctca ttaatttgct tgtccataaa
     2041 cgatttgatt aaagccattt ttcaattcat tattcgctgt ctcgttgaag taaagataat
     2101 tatcgggatt cccctgattt tgttgtgtca ccaatacttt tggctgattt tgctgaaacc
     2161 cgaatagata gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa tagacggctg
     2221 tgatttgata gtctgcttga cctgtcccac tttctgacca ttctgcagaa acaggcactc
     2281 caccaatagc catcttccac tccccgttca aaatagcttg agggacttta agaccatata
     2341 agtccacact catttgattc gtataacttt tgtattgttg tcccattgtt tggccccatt
     2401 tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt tgttcaactt
     2461 gttcgctgct ttctgaagag gaagatgaag cctctacaga agaacttgaa gtagatgttt
     2521 gagttgtttc agtcgttttg gaagactctg acgaagtaga ggtatctgct gtttctgtac
     2581 ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt agttgcaact gttgctttcg
     2641 ctgtttcttt tgttgattct tttttcgatt cattcccgtt ggtacaacca gctaacaaaa
     2701 aagctagacc aattgttact aaaatccttt ttttcaatta aaaaactcct ttcgattact
     2761 cccacttctt tattctatca aataagcaat gtgattttat tgatttttcg tattttctca
     2821 tcaatctttc aaatcttcta aaaactgaga agcacccgct gatgttgtct gtaatacatc
     2881 agcgggtgct tcttttattt taatcttcgt gattttccaa tttacctcac gctttatatt
     2941 tgtatttgaa gaaacataga cttttagtca tcatcgtctt cctcatccaa atcttgtcgg
     3001 acagtttttc cttggagtct ttgtacttgg tagatgattt ctttattgaa acttaaaagt
     3061 tcaacaagca attgttgcaa gccatatttt tcttcttttt cagctaattt gattgcccgt
     3121 gtgataaaga cattttggaa atcaaaatct tttgcaaagt tttccaacat tgttgaggct
     3181 ggtaaatatt tgttttgacc atgttcgtaa atttttccat aacgcatgac ttcttctaaa
     3241 gtagacggaa cgatttcatt ttcaatcact aactcatgag agattttatt gaaccatctc
     3301 gcttcttgat cagcatattc tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa
     3361 acatacaatt gtgcttggat caatttcatt tcttctaatt taaaatttgc taatacatgc
     3421 cccaccatgg cgccagcagt tggtttatga tggtcgatat ctgcttgttt tgtttcttga
     3481 acaaattttt cttccactat attcattgat ttacgcctct tttacttttg ctgattggac
     3541 ttcataacct aatttttcca caactgcagc taattgttca gggtggactt catctggatc
     3601 aaagtttgct ttgattttgc tcgcattaaa caaaactttc gtattgctta ccccaggctg
     3661 ttgagaaacc gcatgttcga ttttttgcat acagcttgga caagctagtg tatctaattg
     3721 cataataatt tttttcatcg tagttcttcc ttcctttttc ttgatagtct tattctagat
     3781 tacagcttcc ataaaaaaat tgattcttat caagttttac gatttttctt ctttgctttc
     3841 gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg atcaagatac tagcttcgtg
     3901 aacgaacatc ccactagcca tgtaaataaa cccggcaaat aagccgttga gtaaaaataa
     3961 gacgacacca atggcaatca ctacattttc acgggtattt aaaacagttt tcttagccaa
     4021 gcggtaggca tgaaccaacg attcaaaatt agattgcatc aagacaacgt cactcgtttc
     4081 gatagcaaca tccgttcctg atcccatagc aatcccgata tcagccgcag ccaaagaggg
     4141 agaatcattg attccatctc caataaaagc aacgcgcaat ccttgttctt ggtatttttt
     4201 cacaaattga accttttcgt ctggtaataa ttcagcatgg acttcatcaa tgcctaacat
     4261 atcagctaca taatcagcag tcatctgatt atcacctgtc agcatcacta actgttttgc
     4321 tcctgcttgt ttcaacttag ctaattgttc agcgacttct ggtcgaatca catctgacac
     4381 acctatgatt tgagtgattt ttttgtcgat tgctacaata atgaccgagc tgcctgtttg
     4441 ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga gaaagtttgt gagcggcaat
     4501 caatttggtg ttgcccgcac aaactgtatg accatcaatc tcagctgtga ttccttgtcc
     4561 cttgattgtc tgattatctt tgactgctaa ttgttgaaaa tcaatgtctt tctcctgagc
     4621 ataatggatc acggcgcgcc ccagaggatg atcggataac gtttctaatc gagccactaa
     4681 agctaagctg tccccttcat cctctgtata gttcttgata gtggatacag cagtagttcc
     4741 tttggtcaat gtaccagttt tatcaaagac aaaagtatcg attttggaaa aagtatccat
     4801 tgcttcaccg cctttgacta agatgccccg tttcgctcca ttaccgattc ctgctacatt
     4861 cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca agaaccaaaa cagtgattgc
     4921 taaacgaaaa tcacgactaa tcagaccaac aaccaacgca ataagtaaaa cggttggagt
     4981 ataatactga gaaaatcgat caatgaattt ttccgcatgg gatttggtat cttgtgcttc
     5041 ttctactaat tcgataattt tggcaaaggt cgtatcatct ccgaccttag ttgcttcaat
     5101 cgtcacgtag ccatctgcta aaatcgttcc agagaagact tcatctccac tgattttgac
     5161 gacttgtcgt gattcaccag tcactgcggc ttcatccgca taccctttcc cgtcaatgac
     5221 tcgtccgtcc acaggaatag ctccaccagt ttttactaag acgatatcgc cttcttccac
     5281 gtcatcgatt tccacttctt cagtcgttcc atcttcttgg acaacactag cagtagatgg
     5341 ggccatctcg gtcaaagcct tgattgactc ccgcgttttg gtcaatgttt tttgttccag
     5401 atagctacca aacaaaaaca agaatgtaac gatagctgac tccttgtatt ctccgatcat
     5461 aaaggcacca agcacagcaa tagtaacgag taaatcaatt gagataactt tgactttcac
     5521 tgcctgatac gcatggatca taataggtat gaccgcaata atagaagcta tggctagagc
     5581 aatgttatag aacaagacaa aaccagtaaa ttttccgata aaaccaaaaa taatcaacac
     5641 agctgataag gcggtgatat gatttcgatg ctcttggatc cacttttgta atttcatctt
     5701 tctccctctt ttcctttcat tcttacgaga ctaagtatag atacttatcc atcataaaaa
     5761 attgaccact atcaagttta gtcaaaaagg cggttttctt ttggtagaat acactacaaa
     5821 aaatagttag caagaagact cactaaaagt gatcttgcca actattgtcg tttctatcga
     5881 aattttcatt gaatgatttt cctgtacagc atacatcgga gatctatttc tttatctaag
     5941 atttttatct tgctaatttt ttcctttagt ctttcaaatc cacgtcgttc atagaattga
     6001 tacgtacagc cactatctgt aaaaaggaat atttcttttc ctttctctct tttttcaaat
     6061 tctgttagca acttggtacc gatgcctttt gttttcaaat caggatttgc agctaaaaaa
     6121 atgatctctc catctggcga atgcttcttt aaatatgctg caagcatttg ttcatttgct
     6181 gtctcgtaag ctcctttact ttctttagca aatatatttt gtaaaaaatc gaatatctta
     6241 acatatattt gctgtccaaa agaacgatgt ttcttttttt ctccttttat ctccgccagc
     6301 aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa tttgagttgc tcgattcaac
     6361 tccatataca aaaaatatct tccgtaaaga ttcagcagaa gttcattttc catatatctc
     6421 tcaaagttca ttccttcaac agcaaaatag atagcttttt tgtaatcttt cttttctaag
     6481 ggtttaaaag ttagtttcat catttcgcct cctcaacttc aatgattgtg ttatacttac
     6541 attataatag acacagtgtc tactatctat aatcaatcat ctataagttt gtcgattaag
     6601 cgacagcttt gctttgatat gtcggataag gagataggta aatgaaaaag acagatagac
     6661 gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc cacattatta gtaaataaaa
     6721 agattcaagc aatcactata cgcgaactaa cagaaacggc agatgtacat cgttcgactt
     6781 tttatacaca ttataaggat atctatgatc tatatgatca attagaaagc aacttttttc
     6841 gggatctaaa tgaaattctc tcttatgatc cagcccatag ctatgaggaa ctatacactc
     6901 gcttgattga ctatgtttac gcaaatactt ttttatatcg cttatttatc ctcaaccatg
     6961 gtgatatttc gtttcagaag aaagttaaac aagttattga agaaaactat ctgaacatat
     7021 ggctatatga agacaagaaa acgattatca cagaagaaat gagattcttt actacgtacc
     7081 acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga aagcaattac aagtactcca
     7141 aaaaagaatt attagaactc ttacagaaac tgaatgatca catagagaaa atcatgcctt
     7201 gaataaaaac atactaagct tagttaagaa gaaccgtaac cttctcgtat aacaactgaa
     7261 tcttatttag ccaaattgtt tactgaagat tttttgagta aaatttctat aattcctact
     7321 tattgaaaag tagtttgtag tgctg
//
LOCUS       DACDKL010000121         7307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDKL010000121 DACDKL010000000
VERSION     DACDKL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.121"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..143
                     /locus_tag="IV329_001995"
     CDS             <1..143
                     /locus_tag="IV329_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941164.1"
                     /translation="KNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKK
                     TL"
     gene            complement(221..754)
                     /locus_tag="IV329_001996"
     CDS             complement(221..754)
                     /locus_tag="IV329_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8941165.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(848..2026)
                     /gene="mutY"
                     /locus_tag="IV329_001997"
     CDS             complement(848..2026)
                     /gene="mutY"
                     /locus_tag="IV329_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8941166.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2019..2816)
                     /gene="recX"
                     /locus_tag="IV329_001998"
     CDS             complement(2019..2816)
                     /gene="recX"
                     /locus_tag="IV329_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8941167.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2923..4305
                     /gene="rlmD"
                     /locus_tag="IV329_001999"
     CDS             2923..4305
                     /gene="rlmD"
                     /locus_tag="IV329_001999"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8941168.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4479..5057)
                     /locus_tag="IV329_002000"
     CDS             complement(4479..5057)
                     /locus_tag="IV329_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8941169.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5379..5849
                     /locus_tag="IV329_002001"
     CDS             5379..5849
                     /locus_tag="IV329_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8941170.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5957..7183)
                     /locus_tag="IV329_002002"
     CDS             complement(5957..7183)
                     /locus_tag="IV329_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8941171.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2113 a         1462 c         1251 g         2481 t
ORIGIN      
        1 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
       61 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
      121 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
      181 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
      241 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
      301 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
      361 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
      421 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
      481 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
      541 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
      601 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
      661 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
      721 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
      781 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
      841 gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca
      901 tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt
      961 tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat
     1021 gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa
     1081 agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca
     1141 gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca
     1201 cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa
     1261 aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct
     1321 ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa
     1381 aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta
     1441 gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg
     1501 cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc
     1561 tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac
     1621 ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg
     1681 tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc
     1741 gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat
     1801 ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg
     1861 tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat
     1921 tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa
     1981 attcttttgt ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt
     2041 tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt
     2101 acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct
     2161 tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc
     2221 gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc
     2281 aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt
     2341 gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt
     2401 ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc
     2461 atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt
     2521 aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt
     2581 cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg
     2641 aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct
     2701 tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat
     2761 aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac
     2821 atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat
     2881 atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc
     2941 aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc
     3001 ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta
     3061 gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa
     3121 acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa
     3181 ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca
     3241 ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg
     3301 gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact
     3361 caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc tataacagac
     3421 tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat
     3481 aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg
     3541 gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa
     3601 ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca
     3661 atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac
     3721 ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct
     3781 cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa
     3841 gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc
     3901 ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca
     3961 atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc
     4021 gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc
     4081 atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac
     4141 cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt
     4201 aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact
     4261 gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg
     4321 aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt
     4381 tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag
     4441 ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc
     4501 acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat
     4561 ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct
     4621 ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc
     4681 caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc
     4741 caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt
     4801 tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta
     4861 tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag
     4921 tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc
     4981 gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac
     5041 gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta
     5101 ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa
     5161 agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat
     5221 caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat
     5281 aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc
     5341 ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc
     5401 gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg
     5461 gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga
     5521 tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac
     5581 ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca
     5641 tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa
     5701 tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc
     5761 atcgcctgac aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat
     5821 tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca
     5881 catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta
     5941 accaaactat ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac
     6001 cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg
     6061 tatagtactg acttgtatct tctgtttgct gataataggt attcggatct tggtactgat
     6121 catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac
     6181 cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt
     6241 tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga
     6301 tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt
     6361 cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat
     6421 caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt
     6481 ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt
     6541 cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat
     6601 aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca
     6661 aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca
     6721 tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg
     6781 aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc
     6841 cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt
     6901 gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta
     6961 atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta
     7021 ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt
     7081 ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga
     7141 atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca
     7201 cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata
     7261 agaaaacttt aaaaaacatt tttgtgtaac gattattatt ttttaaa
//
LOCUS       DACDKL010000122         7270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDKL010000122 DACDKL010000000
VERSION     DACDKL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.122"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..690)
                     /locus_tag="IV329_002003"
     CDS             complement(70..690)
                     /locus_tag="IV329_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8941172.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(766..1203)
                     /locus_tag="IV329_002004"
     CDS             complement(766..1203)
                     /locus_tag="IV329_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8941173.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1366..2196
                     /locus_tag="IV329_002005"
     CDS             1366..2196
                     /locus_tag="IV329_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8941174.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2180..3547
                     /locus_tag="IV329_002006"
     CDS             2180..3547
                     /locus_tag="IV329_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8941175.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3566..4375
                     /gene="yidA"
                     /locus_tag="IV329_002007"
     CDS             3566..4375
                     /gene="yidA"
                     /locus_tag="IV329_002007"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8941176.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4418..4861)
                     /locus_tag="IV329_002008"
     CDS             complement(4418..4861)
                     /locus_tag="IV329_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8941177.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4995..5366
                     /locus_tag="IV329_002009"
     CDS             4995..5366
                     /locus_tag="IV329_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8941178.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5535..6317)
                     /locus_tag="IV329_002010"
     CDS             complement(5535..6317)
                     /locus_tag="IV329_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8941179.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6314..>7270)
                     /locus_tag="IV329_002011"
     CDS             complement(6314..>7270)
                     /locus_tag="IV329_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8941180.1"
                     /translation="FEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEILPDGKG
                     KNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKALINANA
                     VTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVYDILEE
                     RRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVREDAHINA
                     VGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGTLGELV
                     SEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         2202 a         1518 c         1263 g         2287 t
ORIGIN      
        1 atcgtgtcac acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga
       61 aaataatgtt tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc
      121 atcgtcttcg tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc
      181 atctgtcggg tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc
      241 atcttcatca tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc
      301 ttctgggtca tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac
      361 tttcttgcgt ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac
      421 ttcttcatcg atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa
      481 tgagataaag ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat
      541 ttcactgtct gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat
      601 gacatcgcca cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt
      661 gtttgcacct tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct
      721 tatcatacaa aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc
      781 caaggtcaaa taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg
      841 aaccttccct cctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc
      901 aatctggatc ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa
      961 tttcaaacgc atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa
     1021 tttgatcgtt acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta
     1081 cagcgtatcg ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc
     1141 cccttgctgt tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc
     1201 cattattcat tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc
     1261 aaccaacttg tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag
     1321 tataatattg tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt
     1381 cgactagatc agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata
     1441 ttaacggaat attctaaaga acaccagcaa atctttttac atttctggca atatgactgc
     1501 acagtcatct taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca
     1561 ctgaaagatg caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca
     1621 gatgaaggaa ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc
     1681 atagatgacg cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg
     1741 atacaagcat tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat
     1801 ggcaaaaaat ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg
     1861 tacttaagtg tcaacggtaa tcaggaaaaa cgcgggcaag tcatgaagcg attttatcaa
     1921 tctgctttaa aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg
     1981 gcaacacttg aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt
     2041 gcaaaagctt ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta
     2101 aaaaaagaaa tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat
     2161 aacataaagg agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa
     2221 agtttttcgt gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt
     2281 gatcaattcg aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt
     2341 cacttttcac ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc
     2401 aagacgaatc tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg
     2461 ctgggatgat caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca
     2521 tggtccttac tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt
     2581 agagatcatc acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg
     2641 ttttcctgaa aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca
     2701 aatgatttct agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt
     2761 cactggaaca gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta
     2821 tgaaggcggg atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag
     2881 ccgatatcaa atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat
     2941 tttggagcat ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga
     3001 actccattct caattacttc tgccatttct tgacggaagc tttactttag aagaatacct
     3061 caaacttgat gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc
     3121 tattttaaat gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt
     3181 ttctggcaac cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg
     3241 atataatcct gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta
     3301 tcgcccagaa caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt
     3361 gatagaatta tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga
     3421 tgagcgattt ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt
     3481 atttgacgaa acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat
     3541 aaattaaaat atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg
     3601 tactttatta aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc
     3661 tgttgcaaaa ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga
     3721 acagttaaac gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc
     3781 actcgtacaa caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga
     3841 tttcctagag atcgaagtga tggcacgccg tgtcggcagt catctccatt cgatcgatga
     3901 caaagcgatc tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt
     3961 agtcaagatg ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt
     4021 caaaatgatg atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca
     4081 aactttccgt gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa
     4141 taaagaagca agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca
     4201 agatgaaatc atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg
     4261 tctaggagta gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac
     4321 ttcatcaaac gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat
     4381 aaaaaaactg agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc
     4441 atctctcgac tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc
     4501 tcacccacca aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag
     4561 caacaaagaa atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact
     4621 catcagcttc tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct
     4681 cacggacaat cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa
     4741 aagcaccatt tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg
     4801 ctgcataacg ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca
     4861 tgaatagttt ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat
     4921 gaacaagcac aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa
     4981 ggagagatga atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat
     5041 caatcgaatt ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag
     5101 aaaacaaatt tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga
     5161 cttacaacta tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag
     5221 cagctgatga tctagaaaaa ttacacgagc aacataaagc tgccggtctt gagattactg
     5281 acttaaaagg attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt
     5341 ataaaatcga agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa
     5401 aaacaaacac caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg
     5461 tcttttttta acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat
     5521 tcatttgaaa gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct
     5581 attatcacac gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga
     5641 taagctgaca attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt
     5701 gctgatccac atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca
     5761 aagtcttttt gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg
     5821 atagctgtaa aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat
     5881 aaatcacgtg ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac
     5941 aatatcggca cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt
     6001 cttgtcacaa atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg
     6061 taatagttaa ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct
     6121 cgatctggcg taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt
     6181 tcagataaat tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct
     6241 agcacataaa ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg
     6301 taataatcta agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac
     6361 aacaaagatc ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt
     6421 tataaaatgt ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct
     6481 ctaaatctcc ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt
     6541 aatcatctac atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag
     6601 gagtaaatgt gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa
     6661 atgcctcttt actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta
     6721 ctgatattgc atttataaac gttattttag gatactgttt tttagcttta gcgataaaag
     6781 tatttcgtct ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag
     6841 aaatcgcagt caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg
     6901 catgcggatt tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg
     6961 tgactgcatt tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa
     7021 tctctcctaa atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt
     7081 ttgtgccaaa atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc
     7141 cttttccgtc aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat
     7201 aatcaaaaaa acattttttc attacttgaa tcgcttcttc aaaactaaaa gaagctacta
     7261 cttcctcaaa
//
LOCUS       DACDKL010000123         7159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDKL010000123 DACDKL010000000
VERSION     DACDKL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.123"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IV329_002012"
     CDS             complement(<1..179)
                     /locus_tag="IV329_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8941181.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IV329_002013"
     CDS             complement(192..1295)
                     /locus_tag="IV329_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8941182.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IV329_002014"
     CDS             complement(1385..2320)
                     /locus_tag="IV329_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8941183.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IV329_002015"
     CDS             2622..4556
                     /locus_tag="IV329_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8941184.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IV329_002016"
     CDS             5013..5429
                     /locus_tag="IV329_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8941185.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IV329_002017"
     CDS             5443..6009
                     /locus_tag="IV329_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8941186.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IV329_002018"
     CDS             6032..7018
                     /locus_tag="IV329_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8941187.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         2083 a         1381 c         1467 g         2228 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttat
//
LOCUS       DACDKL010000124         7147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDKL010000124 DACDKL010000000
VERSION     DACDKL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.124"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..347)
                     /locus_tag="IV329_002019"
     CDS             complement(<1..347)
                     /locus_tag="IV329_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8941188.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIV"
     gene            complement(369..671)
                     /locus_tag="IV329_002020"
     CDS             complement(369..671)
                     /locus_tag="IV329_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8941189.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            complement(728..1207)
                     /locus_tag="IV329_002021"
     CDS             complement(728..1207)
                     /locus_tag="IV329_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8941190.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            1372..2145
                     /locus_tag="IV329_002022"
     CDS             1372..2145
                     /locus_tag="IV329_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8941191.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            2165..2593
                     /gene="lacA"
                     /locus_tag="IV329_002023"
     CDS             2165..2593
                     /gene="lacA"
                     /locus_tag="IV329_002023"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8941192.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            2612..3127
                     /gene="lacB"
                     /locus_tag="IV329_002024"
     CDS             2612..3127
                     /gene="lacB"
                     /locus_tag="IV329_002024"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8941193.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            3140..4066
                     /gene="lacC"
                     /locus_tag="IV329_002025"
     CDS             3140..4066
                     /gene="lacC"
                     /locus_tag="IV329_002025"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8941194.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            4079..5065
                     /gene="lacD"
                     /locus_tag="IV329_002026"
     CDS             4079..5065
                     /gene="lacD"
                     /locus_tag="IV329_002026"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8941195.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            5521..6189
                     /locus_tag="IV329_002027"
     CDS             5521..6189
                     /locus_tag="IV329_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8941196.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            6180..>7147
                     /locus_tag="IV329_002028"
     CDS             6180..>7147
                     /locus_tag="IV329_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8941197.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLK"
BASE COUNT         2295 a         1227 c         1598 g         2027 t
ORIGIN      
        1 atgacaatat tgacgatcac cataattagt aaaaaataaa gcgtgtatgg cgacccccat
       61 gtgatggttg ctagtggggc ccaaccgaca tctgtaatgt ttaatgaaat acctgtgcgt
      121 tcaacaaaag ctgtcatagc gtctgaaaaa gcagtagtta aaattccaat aatagctccg
      181 atcccagtca gggcgatccc taatttgagc ccgccttcaa gagcgcgtga cggttttact
      241 ttaaaaaata aagcaataac agttaatacg atggtcatta acggtgccgc acctaaatta
      301 attagggggg taaatacttt attggcaata tcaataattg tattcatatg cttttccttt
      361 ctttagtcct atttgtttaa tgattggatt gcgctttcca tactttggta aacaggctca
      421 gccatagcag gaatacgata aagaatagca cctgcatcaa cgacagggat gttaatgtca
      481 aaacccaagt cggtgttggc aatttgtgcg aaaatgtcat aatgagacag catttcttca
      541 ttgatatctt tgatcataac ggcgtcacag gtgacttgat agccacgatt tttcaattca
      601 gtttccaaag cgcttttgat ttgatggctt gaattaacgc ctgctccaca tgctgctaaa
      661 attttgatca taataattct ccccttaaaa taagtttatt ttttatcctg cggttaggca
      721 gagacttcta tatataaaaa tgttcttcta aaaaatgata aatcgcttct gtgtcctcta
      781 atttgaaaaa cgaaaacagt ttttcttcgt caacagaatt aatgaaatcc atgatattag
      841 ccaacatgcc agcctgtgct tcaccatttt cgttcaagat catgaaaaga aatgaagcgg
      901 aaatctgatc gcttggcgag atcatgttgt agaaagggat ttcatttttt actttgattg
      961 gaatgatacg ggtggttcgc acaaactcac tttcggtatg agggatagcg atatctggca
     1021 atattttaga gacaggtgag agatctaatc ccgtcggata attacgctcc cgttctaaaa
     1081 ggttctccaa aaaatcttcg gttaccaatt ctttctttaa cagatcttga tagacttcct
     1141 cgaacacttc ttcctgcgac tgcttgtcag aaacatagac agtgtctttg aaaaagaatt
     1201 cattcacaca aaacagctcc tttattagtt cgtttatgtt tatttatatg tttacaagtt
     1261 gagtataacc gcttacaaaa tcattgtcaa cgcataaacg aaaataaacg aacaatatgt
     1321 gttgaaaaat gttcgtaaat gttttattat aaggtcgtgg aggtacagaa tatgctgaaa
     1381 acagaacgac agcgttcgat cttagcgatg tgtgatcgct atgggacgat aacagtcaaa
     1441 tttattcagg aagtactgaa tgtttcagat atgacgatcc gccgagatct agaggaactc
     1501 gcgcagaaga aaaagctttt acgcgttcat ggcggtgcac aaaaaatcga ttatctggaa
     1561 atgtcgactt ctatagaagc aaatgagctt tctcatactg aaaaaaagaa gctgcataca
     1621 aaagagaagg aatacatcgc taaaaatgcg gcagctcata tcaatcaaga agacgacacg
     1681 atttttcttg gatcgggtac aacgatcgaa ttaatgacac gatacatcaa gccgtcatct
     1741 ctccgaatcg ttaccaatag tctgccggtt ttcaatctat taagtgaaat aaaaagattt
     1801 gaactgtatt tgatcggtgg gctatatcgg gaaaagactg gagcctttgt cggttcaatt
     1861 gccaatgaag ctgttcaaaa aatcggtatt aagaaggctt ttgtcggagt gaatggattg
     1921 aagggttcgg aggtttcgaa tttcagtatt gaagaaggta ccttgcaaaa aattgtttta
     1981 gataatgcgg cagaaaaatg tttggtttca gatgcaaaca aattcgacca tagcgatttt
     2041 tataatttct atgatttgcg caagatcgac aaattattta cggattctac tctttcagcc
     2101 gagctgaaga gttattatca gcaattcact aaaatcattc agtaaaaaat aggaggaaac
     2161 aaaaatgcgt gtagtaattg gttcagacag tgacggcatg gaattaaagg aattaatcaa
     2221 gaaaaaattg atcaaagaag gtattacagt agtagataag agtgaaacgg cttcagaaga
     2281 ttttgtggaa tcaactttgg cgatcgcaaa tgatctgaat gaaaacgaag gtagtttagg
     2341 aatcgcggtt gatggctacg gcgctggcag ctttatcacg gcaacaaagg ttaaaggaat
     2401 ggtagcagca gaagtttcgg atgagcgcag tgcgtatatg acccgtaaac ataataattc
     2461 tcggatgatc acacttggat caaaaattgt tggcgatgag ttggcgttga atattgttaa
     2521 ggaatttcta caagcaaatt atgatggtgg tcgtcatcaa gtacgtgtcg acatgttaaa
     2581 taaaatggcg taattaggaa ggatgaatga aatgaaaatc gcaattggct gtgaccacat
     2641 cgtcacagat acaaaaatgg cagtatcaga tttcttaaaa tcaaaaggac atgaagtgtt
     2701 ggatatgggg acctatgact ttaccagaac ccattatccg atctacggaa aaaaagtcgg
     2761 agaggccgtg actagcggag aagcagatct aggagtttgt ttgtgtggaa caggtgttgg
     2821 gattaacaac gctgtcaata aagtacctgg tatccgcagt gcgctggttc gcgatatgac
     2881 gactgctatc tatgcgaagg aacaactaaa tgccaatgtg atcggctttg gcgctcggat
     2941 tacgggagaa tttttgatct gtgacatatt agaggccttc atcaatgccg attatcaaga
     3001 aactgaagaa aacaaaaaat tgatcgccaa aatcgatcac gtcgaaaaag ccagtgacaa
     3061 gcagtctgat ccgcatttct tcgatgaatt tatcgagaaa tgggatcgtg gcgaatacca
     3121 cgattaagcg ggtggacaaa tgattttaac aataacgatg aatccatcga ttgatatttc
     3181 ttatccatta gaaaccttca agctcgacac agtcaatcga gtaagaaatg ttcgcaaaac
     3241 agctggcggt aagggcctaa atgtgacacg tatcttgaag caattagata cggaggtgat
     3301 tgctagtggt ttaatcggcg gttttttagg tgaagagatt aaaaaggacc tgactcaaaa
     3361 tgagattgct catgactttt tacctatcag cggagagaca cgtaattgca tcgctgtttt
     3421 acatgacgga aaacagacgg aaatcctaga aaacggacct gttattactg aacaagaagc
     3481 aagggcgttc ttgcaacatt tcgaaaaatt agctgttaaa gccgaaatca tttctttttc
     3541 aggaagcttg ccagaaggat tgcctgctga tttttatcga caaatgctgc aagtttgtca
     3601 gccaaagcct gtcgttttag attgttcagg tacagcatta agggaagtgt tgcgaggcag
     3661 tgtaaagcct aagctaataa aacccaatac cgaagagctg gctggtctgc taggacgaga
     3721 aatctcaaca gaattatcag atttaaaagc cgccttgtca gattcgatgt ttgatgaaat
     3781 tgaatggata gtggtttcaa tggggggcga cggagccttt gccaaacatc acgagacttt
     3841 ttattgcgtg acaattccaa agattgatgt cgtcaatcca gttggctctg gtgatgcgac
     3901 cgttgccgga ttgaccgctg cgattcaaca aggtctttcg gatgaagccg tgttaaaaca
     3961 gggtaatgtt ttaggaatgc tgaatgctca ggaagcgacg actggctatg tccaattagc
     4021 caattatcaa aaactatatg atcaaatctt agtggagaaa gtgtaggagg atacggaaat
     4081 gaacgaagaa aaacgtcaat atatcaagca actatgcaat gaagatggag tgattggggc
     4141 cttagcaatt gatcaacgcg gggctttgaa gaagatgatc acaaaattca agggcagcga
     4201 agcagaagaa tcagaaatcg ttgaatttaa agaaattgtt tcttcacaat taacgaaata
     4261 tgcctcatcg attttattag atcccgaata tggcttgcca gcagctgacg ttagagcaaa
     4321 aaatgccggc ttgctgttag cttatgaaaa aactggttac gatgcgtcaa ctcccggccg
     4381 tttaccagat ttattatcga tctggtcagt taaacgttta aaagaagcag gcgccgatgc
     4441 ttgtaagttc ctactttact atgatgtcga tgaagcagaa gaaatcaata atcaaaaaca
     4501 tgcctttatt gaacgaatcg gctcagaatg tcaggctgaa aacttaccct tcttcttgga
     4561 gcttgtttct tatgacgcga acatctctga tgcgacttcg aaagaatacg ccattaagaa
     4621 acctcataaa gtcaacgata tgatgaagga attttctgat ccacgttatg gcgtagatgt
     4681 gttaaaggtg gaagtacccg tcaatatgaa ttatgtggaa ggctatggga cagacgaagt
     4741 cgtgtacaca aaggcacaag cggctaactt cttcaaggaa caggctgaag ccactaacct
     4801 gccatttatt ttcctaagcg ccggtgtaaa agcagagttg ttccaagaaa cactgcgctt
     4861 cgcgaaaaat gccggctcaa ccttcaatgg tgtactttgc ggccgtgcga cttgggcaga
     4921 tggtgtagaa agctatatcg ttgatggaaa agaagcagcg atggagtgga tgaatacgca
     4981 agggcgtaaa aatattgaag aattaaatgc cgtattgaaa gaaactgcta caccggtgga
     5041 agtgttaaca agctcactat cataagaaat cagactgtga agcaaaattg cttgcagtct
     5101 ttttttatac ataaagcgta aaatcatgct gcattattag gggaacgaat tctaaaatag
     5161 taagtgagtt aaaacgaagg ggtttttctt tagagagatt ttcttacatg tggtacttaa
     5221 tgacttgatg gcaattttca gtattctaac gaggcttatt ggtcataaac caacgaactt
     5281 tgaaaagtat atacaagaaa accaagcaaa tagaaagtag tgagactgga ggaaaggttc
     5341 cagtcttttt ctattctagg tattttcaaa cagtaaaatc attcttaatt atgtgttttt
     5401 taacagaatg tatgaatttc ttcaaatctt cacaaaaaga acgtggactt tgaagaagag
     5461 cgtgtactat gctaaaaagc ataagtgtaa ttactaaatc cgtatgagga gtgaatatac
     5521 atgcgtgtac tggtggttga ggacaatcgt gagctggttt tatccgtaaa aaagggccta
     5581 gagcaggaga atatttctgt agatgtagct tatgatggcg agtcaggaga agagaaggcc
     5641 ttcatcaatt catacgatgg gatactacta gatttaaatc tgcctaaaaa agatggatta
     5701 tctgttttac aaagtctccg tgctgaagaa gtcgaaagtc cagttatcat tattactgca
     5761 cgtgatgaga ttgaagaacg agcaaaaggc ttggattttg gagcagatga ttatttagtc
     5821 aaaccctttg agttactaga attaaaagca cgaatacgcg cggttattcg tcgttatcat
     5881 ggacgtgcgc tgccgcagat ttcaattggt gctttgaaga ttgatccatt aggtcgaagg
     5941 gtcagctatc aaaagacaga ggtggtcctg aaacccaagg aatttgatat cttactgtgc
     6001 atcgctcagc gtcatccagc ggttgtttcg tctgaggaaa tttctgaaca tgtctacgat
     6061 gaagattttg atcctttttc gtcggttctg cgagtgcatc tggctcgtct aaaaaagaaa
     6121 ctggctgaag cagctgaaaa agaggtctta aaaaccattc gagcaaaggg gtaccagcta
     6181 tgctattaaa ttttaaaatg acgttaacga tgctggcttt ttcactgttg gtgacaggcg
     6241 tgattttagt gagtttcttc tttctgagaa ataatttgga gggaatcagt atcgcatcta
     6301 ccttaggtga agatgtttat tttatggaag agggtaagac ggttcaagca gagacgcaga
     6361 tggaagcttc gaaatcttcc aacaatcaat ttatgactat ttcgatgata gataaagctt
     6421 accaggacga actatttaaa tatttaccag cgatggtgct ggttatttgt tctgccgtca
     6481 tgttattaac gtttattctt tggttggttt tacgcaaaat ttacagtaat caaatcttag
     6541 cgattacgcg tgatttaacg tttattgatc aagttcctaa aaatactgtg aaggacgagg
     6601 cattaaatgc ggcttttaat aagctaaagg ataagttcga tggtcatttg gaggattatc
     6661 gacgattaaa tagttatctg acccacgagc agaaaaatgc catcgctatt ttgcgtacga
     6721 atctggaaat agaacggcaa catgccgatc aaggaaattt aatgctttta gatcggatga
     6781 ccgacagtat tgacgatatt ttgacattat ccaataactc agaggatctt agcacggaag
     6841 tcgtagacgt ttcactgatc tgtgcggaag tttgtgatgc ctacaaaatg aattatccga
     6901 atcttttgtt tgcgtttact gaagatcaac caaccctgat tcatggacga gatcgttgga
     6961 tttatcgggc ggtttcgaat ttagttgaca atgcaatcaa atatggtgaa ggaaaactgg
     7021 taattgtcac catttcaaac caaaagggta gcgtgattat tgaagtaaaa gatcagggaa
     7081 ttggcattcc tcaggagaaa caggcatcaa ttttttctca tcagtaccga gtgcatgggc
     7141 tcaagca
//
LOCUS       DACDKL010000125         6836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDKL010000125 DACDKL010000000
VERSION     DACDKL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.125"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2872)
                     /locus_tag="IV329_002029"
     CDS             complement(<1..2872)
                     /locus_tag="IV329_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8941198.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVI"
     gene            complement(2886..4103)
                     /locus_tag="IV329_002030"
     CDS             complement(2886..4103)
                     /locus_tag="IV329_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8941199.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4093..5688)
                     /locus_tag="IV329_002031"
     CDS             complement(4093..5688)
                     /locus_tag="IV329_002031"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8941200.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            5813..6598
                     /locus_tag="IV329_002032"
     CDS             5813..6598
                     /locus_tag="IV329_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8941201.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1996 a         1391 c         1135 g         2314 t
ORIGIN      
        1 taatgacaaa gatatcttca ttactatcta aagtccccac atcaaatgca tttaaaattt
       61 gtcggtaaac ttctttgact ccttctgttt tattcaattt agccaaagct ttttcaattt
      121 cttctcgtgc atctgaacta ttcgcagcat aagttcctag taattgatca atataagtag
      181 aatcaatatc atacaactta gtggtatttt cactataaaa ttcgatcgtt gaaaaatctt
      241 ctccctcttt ttctggttct tcctccatca atgaaccctt cacggtctcg tacacgccaa
      301 tttgttcttc caaaagttga atttgattgt tcaaagttga agattgcacc atgtcatcat
      361 tgtaatcatc ataagtcact aacgattcat acgcacgatt caattcttga aatgctttga
      421 cgtactcgat acggatttca agtggcgtat gttcatcaat ggattccatg gatgaaacct
      481 cttttaactt accatatgct tgcttgaatc tttttttgga ttcttggtaa cttggataaa
      541 tcaaacccga ttcttcttta gcttctgaat acagttttgt cgcatcctcg acattcttct
      601 ccattgtcgc tggctttcgg aaagtaacaa ttagtccttt tgttttttca ggatacgtac
      661 ggttcgtacg tgaaaaggct tggatcaatc ctgcatactc tagattacga tccacaaata
      721 gggtttgaat cgtaggggcg tcaaaacctg tcaatagacg atccactacg attactaaat
      781 cgatttgttt cccaaattgt ttgaattccg ctctttttcg tgccaaacga ttattgatgt
      841 ctccgttata acgctcaatg tctgctagtg accaggcagt atcataatac tgattatatt
      901 cctcaataat tttttgcatt tcttcttgtt gggcacttga atctcggcta ttttcttcta
      961 atgaataggt aatagcgatt cgtgggaaat caggatcctc catcgttcgt ccttcacgga
     1021 taggctgatt gggaaactct tttattaacc aatcagattc tttcgtcatc tctttgatgg
     1081 cccgataata ctttttggcc atatcaatgg aactcgtggt taagatcgca gacttcgttg
     1141 gacggccatt tcgaaaatca aattttgtat aggcattgtc tggacggaac attttatgaa
     1201 tgactttttg gatatgttcc tctgcttcaa aaacagtagg gtccaaataa ctttcttttt
     1261 tgacagactc catttgatcg atcatacgat tgatctgttc cggtgaatac tctgcatatg
     1321 tttctacttc ttttaatttc cgatagattt tattctctaa tgaagtcggc tccacggtgt
     1381 tttcatgctc tacttgaaat cctaaaaccg agccatcttc taatgcattc ttgatggtat
     1441 aggtgtgcaa gacctctcca tactgatcat gggtcgtacg tgccaattgc cctttggctt
     1501 gtttccgatt ctcagggaaa atcggtgtac ctgtaaagcc aaaccaagtg gatttcggaa
     1561 acattttttt gatttcttcc atattttcag cacttaacgc tcgatgacac tcatcaacta
     1621 caaacacgat atgttgcccc attaattttt ggaagcgatt ggttcctttt ttctcttcct
     1681 gtttctttgc atatttcaac gccgcatcta gtttttgacg ggttgtaatg atcacaacat
     1741 tcgcattggc atctgccaac agagtttcac ttaactcttt ggcacttcct gttcccacga
     1801 tcaagctatt cgctttggca ttaccagaag aaaccccagt attaaactcc gaagcaaatt
     1861 tcgtaaactc tgtggtggtc tgattatcta aatctttgcg gtccactaac atgatcgttc
     1921 gatcgatccc cgattttcgt gctaaaagct tagtagatac aaaactagtc aacgtctttc
     1981 ctgaaccagt cgcatgccac acataccctg attgatgttt atttgcggca ataaacaagg
     2041 cttgaatcgc atgcacttga taaggatgta acaccatgag ggttttattg tcttgatctt
     2101 cactgacaat cgtataatcc gcaatcaagc gatgggcatc tgggatattt agcacttgtt
     2161 tgcaaaactc atataagttt tctacctttt tattatcttt cgttcgccaa ctaaataaaa
     2221 acttaggatg caaatccttc ggtaaggcat ttgcaaaata ccgagtcgtt tgttcattgg
     2281 aaaccacaaa caactgtaag gtcgaaaaga tattgttccg aaacatgcct tcttcagcat
     2341 attttttgat ttgattaaac gcctgataaa aaccatcttt tgcagttact tgttttagct
     2401 cgatttggat aatcggtaag ccattgatca ataaggtgac gtcaaaacgt cgatcccgcg
     2461 cttctatgtt acttccacgt tttgcaattt gatggaccac ttcataagta gagattcctc
     2521 caccaatgtc ttgattggaa taaaggataa gtgagacaga acccaattgc ggattttccc
     2581 gttcaatcgt gatccgagcc atcccatttt caccttttaa ccatttcgca gcatcaaatg
     2641 gggttttcgt ccgtagcaat aattcggttt tgatcgtttc aaactcttta tcggtcaaag
     2701 gagaatcatt cagttctgct tgattatttg agatgatttt ttgacgtaaa ttgctccaaa
     2761 gatcttcttc tgattttaga tccggacgat acgtccactg attatgtcct tccccgagga
     2821 cacgaatcaa ctgttcttcg acttccgctt cctcacggtg actaattttc attcttcttc
     2881 cctccctaaa caaacatctt ttgcaagaag ccttttttcg tttccttcag caaatctaac
     2941 ttacgttgat gaagagcgat cgtatcatct agctgcttga agaaggcacc aattttttgt
     3001 tgttcattta tatccggtgg aaactgaaat gtcctttgtt ctataccacc tttagtcaca
     3061 tgtaccattg tcgttccgtt agttgattct ttaattttct ctttatcagt caatagccaa
     3121 gtatatagat actgtttgtc caattcctta gataaaaatt taagtttcca aatatgataa
     3181 tggtagatta cacgttcttc attccacatt tcaggaccga agcttgttgc ccataaatat
     3241 aagagatctc ctttattagc atacttgtct tcctctaatt ctaagtttga ataataccat
     3301 ctatcattag tattaaaatt acctactctt aaaaccctat attttccaga atcaagtaat
     3361 tcttgttgtt taaaagctct accattcaaa aaaactacca cttcccccaa cttccgctct
     3421 tcccaatctc ccgtaaatcc tggaaaacga acttctggca cttttgctcc gtttttaggg
     3481 aacatttttt gtaagaatcc ttttttcgtt tccttcagca aatctaattc gtgctggtga
     3541 agcatgatgg tgtgatcaag ttgtttgaaa aatgaaccga tcttgatctg ttcgtcataa
     3601 gttggaagtg agataggtgt ctttttacca ctatcaattg tataggttcc gacggttcca
     3661 atgcctgcaa cgtcacggat atacttctga attttgttat ttatgaacga gtagtaaaag
     3721 aagtttccgt caattttatg ctcattcttg aaccaaatga tgtttccatc cttaaagtaa
     3781 attgggttat catcagtgat taataagggg acacctattg atccgacacc agttacaagc
     3841 aagtctcctt gagaaacttt acctgaattt ttcgaatatt cattatattt ttcttctgaa
     3901 atgtaaagat aatcggatgg ttcctcattt tttgcagctg agacaatatc acgagcacgt
     3961 aaaaatctaa tacctgagtt agtccaatct gattgatgaa ttcgcttcac agaagtaaca
     4021 tccatcatat ctcctaactt acgctcttcc caatcttccg taaaaccagg aaaccggatt
     4081 tctggtcgtg tatcattgct catgtttgaa cacctccagc gcaccttgta accacgcttc
     4141 attttcaggt gttgtttgca gggaagagat agcttctaaa agttcttttt ctagcacttg
     4201 ctgttcttgt cgtatcttct tgatgtcatt tccgacatga accatatcaa ttggttcttc
     4261 ttcttcgaaa gtatcgacat agcgtgggat atttaagtta tagtcatttt ctttgatttc
     4321 atcaaaggtt gccaaatgag cgtatttctc gacatctttt ctttcagcgt aggtttcgag
     4381 gattttttga atgttttctt ccgataattt attctgattt ttccctttaa caaattcacg
     4441 actagcatca atgaataata catctcgagt ttgacgattc ttcttcaata caatcaccgt
     4501 cgttggaatc gatgtaccaa agaacaagtt cgcaggcata ccgatcacag cataaatact
     4561 gccgtcttct aataattttt gacgaatgac cccttctgca gcaccacgga ataaaacccc
     4621 gtgtggcaag acgatagcca tagtccctgt ttccttcaaa tgatagaaac catgaagaag
     4681 aaacgcaaaa tctgctttgg attttggcgc taatttcccg tagcgattaa accgagaatc
     4741 atctaagaag gtcgtatctg ctgaccaatt agccgaataa ggtggattca tcactactgc
     4801 atcaaatgtg taaggttcat ctgttggcca gtccttattc aacgtatccc cattgcggag
     4861 attcatttct tcggcatcta ccccatgtaa aatcaagttc atttttgcta gattgtatgt
     4921 cgttgtattt agttcttgcc catgatattt aacgttatct ggatgagtta agtagttacg
     4981 tacatttaac atcaaggaac ctgaacccat ggttgggtca aagacactaa agaatcgtcg
     5041 ttctttttga tccaacgtca cgatttgtgc catcatatca gaaaccatat gaggcgtgta
     5101 aaattctccg gctttcttcc ctgcttctga agcaaattga ctgatcagaa attcatacgc
     5161 atccccgatc acatccccat cgtgacctaa aacttccacg tcattcaatt tcttgataac
     5221 ttcggtaatc gtcacatttc gttgttgctc atctgttcct agtttttttg attggagatc
     5281 cacatcatca aataacccat taaattgatt gtaggtagat gccaattgca caaaggcttt
     5341 gtttagatcg ttcaattgga acgttgcttg ttttgcttga tccgctaaga cgttgaaaag
     5401 atactctggt gagatcgaat accccaaaat atcaacgatt gtggcaatca ggtcttcttt
     5461 tgattcttca tctgaaagta attcacgata caacattgtt tgtttgctta ctgtgtcata
     5521 ttcctctaag gattcatctg ctaaaagaac cacttgttcc aacaaacggt cggataaata
     5581 tttatagaaa atcagtccca ataaataatt tttatattct gacgcatcca ttttactacg
     5641 gagattatct gccgcactaa ataatttttg atttaattct gcacccaaaa agatacactc
     5701 ctatctctta tttaaagttg ctatataccc tcttatttta gcatactccc ttttagaaaa
     5761 agcaaaaaaa aaagaaaaaa tcttattctt gataacaagc tacgtgacgt atatgcctaa
     5821 caaaaataga acttttgaat caatcttaaa tgaaggttta accacagaac cggcatccat
     5881 tatttttgat gaagttatcg atgacgttga agatagtaag tatcataaaa atttaaaaga
     5941 acttttttat tttgcactca acaatggacc aagtttgttc actggtatat cagtacctga
     6001 tagtgataat ccattaatta tggcaaaaat ttacttaaca aaatggtgta ataaatacat
     6061 cagggatagg aataacccag cattaaaaaa acctcttaaa acatttggcg aaaaagatgc
     6121 tgctttaact actagagtag cagcaaatgc taatattgat gatcaaaaag tattgaatga
     6181 ctacttgcgt ggacattttt tatatatgag cgctgagaac atgaacggaa gtattttaga
     6241 ggaatattta gccgaagtgt tagaacctga aggttggatt tggtgcgccg gctctgtata
     6301 tcgagctgtt gatttctgtt atttaggaac ctcaccaatt cttttacaag tgaaaaataa
     6361 atacaataca gaaagcagtt cgagttcagc tataagagta ggaacaacaa ttagaaaatg
     6421 gaatcgttta aataagtcga caaaaatttc tggtttggat agccctatcc ccaattggaa
     6481 agcattaata gaaatgactg aagctagtaa ggaactggca gctaagttaa ctgaaaatag
     6541 ttatttagct tatattaatg aaaaatccac acgagaatta tggacactcg atgactaaca
     6601 atatccttat tagtatctat ccacgataca tggaaaaaat tataaacaaa caaaaaaact
     6661 ttgaatttag aaatttctat cttacctcag atccttataa tatcttttgg atttacgaaa
     6721 caatccccac caaagcttta aagtataaat tgattgtaaa aaatccaatt actaaactta
     6781 agaaaaatca acactacttt ttaggagacg atgagtttga acaattagtt taaaag
//
LOCUS       DACDKL010000126         6753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDKL010000126 DACDKL010000000
VERSION     DACDKL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.126"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..794
                     /locus_tag="IV329_002033"
     CDS             <1..794
                     /locus_tag="IV329_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8941202.1"
                     /translation="SVMIATALENCNKTRRIKFYVIDDGLSEYSKEGLEETVNKYSSN
                     ASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLLAKEDYKKVLYLDSDVLVLDDIV
                     KLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYFNSGVMVIDIDQWNKKEITEKTI
                     HYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQTSLIFERHPAPNEKYERQYKEG
                     NEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHSTLTKVGEINE"
     gene            787..1689
                     /locus_tag="IV329_002034"
     CDS             787..1689
                     /locus_tag="IV329_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8941203.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1722..2072
                     /locus_tag="IV329_002035"
     CDS             1722..2072
                     /locus_tag="IV329_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8941204.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2106..3043
                     /locus_tag="IV329_002036"
                     /pseudo
     CDS             2106..3043
                     /locus_tag="IV329_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            3121..4014
                     /locus_tag="IV329_002037"
     CDS             3121..4014
                     /locus_tag="IV329_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8941205.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4052..4615
                     /gene="amaP"
                     /locus_tag="IV329_002038"
     CDS             4052..4615
                     /gene="amaP"
                     /locus_tag="IV329_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8941206.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4629..4817
                     /locus_tag="IV329_002039"
     CDS             4629..4817
                     /locus_tag="IV329_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8941207.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            4830..5732
                     /locus_tag="IV329_002040"
     CDS             4830..5732
                     /locus_tag="IV329_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8941208.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5764..6006
                     /locus_tag="IV329_002041"
     CDS             5764..6006
                     /locus_tag="IV329_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8941209.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6262..6633)
                     /locus_tag="IV329_002042"
     CDS             complement(6262..6633)
                     /locus_tag="IV329_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941210.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2452 a         1158 c         1344 g         1799 t
ORIGIN      
        1 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
       61 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
      121 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
      181 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
      241 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
      301 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
      361 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
      421 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
      481 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
      541 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
      601 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
      661 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
      721 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
      781 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
      841 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
      901 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
      961 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
     1021 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
     1081 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
     1141 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
     1201 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
     1261 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
     1321 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
     1381 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
     1441 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
     1501 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
     1561 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
     1621 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
     1681 gttaaataat agaagacaac aactgaagag ggggagtagg aatggaaatt acaatgtcta
     1741 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
     1801 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
     1861 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
     1921 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
     1981 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
     2041 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
     2101 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
     2161 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
     2221 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
     2281 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
     2341 atcagtcaga taatcgtatc gaaaaaaatt aacgccaata tcttttgtac tttccgataa
     2401 tctcttgatt acttgtatcg atagaaaaac cgaatttgtg gatcggctgc tgacaaagca
     2461 tggaaatcat ttaaactcgt ttgaaaagat catcacgtat acattattag atatttacac
     2521 ccattacgtg aaagaactaa gagagatgaa aaagcgaatt gacgctttgg ataaagcagc
     2581 tcgtaaaaca acggaaaatg aagaactgta taaacaggca gatcttgaaa gagacatcgt
     2641 atatatcgat cataccttaa gagatcaacg ggaaacaatg gatcaattat ggaattctga
     2701 aactttcatc aaacgtttag acgatgaaca gctactctat gatgtccatt tgagacagcg
     2761 acaaacagaa aaaatgatcg agatctatcg agatttatta gagagtattg gtgatctatt
     2821 caacgggatg atggacaata atctaaatca tatcatgaaa tatctagatt cagctgcatt
     2881 aatcatttcc gttccggcgt tgttcgcagg gatttgggga atgaatacag gaggattacc
     2941 gggtaaaagt tctgctctag gctttatact cgtagtcttt ggctctttga tagcagcagt
     3001 agttttaggt taccatttat accggaaaga ttatacaaat taaagaatct atttaaaaca
     3061 ggtatcaaac aaagaaattt gttttggcaa tacaatttaa aaactaggag gaatacagca
     3121 atgacagaac caaaattaat cgacccgcga aacttgtacc atacggagaa atttcctaaa
     3181 caagatcagg atacaccagc tttacagcag aatatgattc ctgttcctga ttgtggagaa
     3241 gaatcctatg aaggcaataa tcaactagaa aaccgccgtg tactaatcac tggtggagat
     3301 tcagggatcg gtcgtgcagc agcgattgca tttgctagag aaggggcaga tatcgctctt
     3361 caattcttcc caggagaaga aaaagatgca gaggaagtag caaaatacgt gaaagttgca
     3421 ggacgtaaga ttgtcttact accttttgat cttcgtgacg aacaagcacc taaagaaatc
     3481 atagataaag ccgtgagtga acttggcggg ctagatacga tggtactaaa cgcaggacag
     3541 caaatttctt gtcagtctat tgctgagtta ccacttgagc aagtcaaaga cacatttatg
     3601 ataaacatca tcgctatgtt tgctttagtg aaagaagctg ttccgcatat cccagcagga
     3661 ggagcaatcg taacaacaac ttctgttcaa gcattcaatc caagcgaaca tttattagat
     3721 tacgcagcaa caaaagcttc aatcgctaac tttacgatcg gtttagcaaa acagcttgca
     3781 cctaaaggaa tccgtgtaaa tggtgtagca cctggtccaa tctggacacc attacaacta
     3841 gacgaaggac aaccttctga ttcgatccct gaatttggtc agcaaagttt actagaacgt
     3901 gcaggtcagc caggagaatt agcaccagtt tatgttttct tagcatcgaa taaagcaagt
     3961 tatgttacag cccaaattta cggagtaact ggtggagaag cgatcaactt ataagaagtt
     4021 gatcgtttca cccctatgag gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt
     4081 gatcggaaca ttacttttgc tatctgtgct tttgtttgcg atactattaa gtgcaaccta
     4141 tccattgcct tttcagctag agaagtttcg attcttttcc atcactaatt catacgtgca
     4201 acaatatgta ttttggcttg ccgttgcatt tgctattttg acaattatca cgttacttgt
     4261 tttcctgttt tatccaaaaa caatcggaaa ctttctactg aaacatggtg atggggagtt
     4321 gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg aagatgaatt
     4381 catccattca ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa
     4441 aggggagttg aaacgaactt cagcactcat tggtaaaaca ggaacattga tgaaagaaat
     4501 cgaaaatgaa gtacaacaca tattgggaac aaaagagcca gtgaaagtat cggtgaaata
     4561 cacaggcttt gaagaaccag ctaaacgaag caatgaccgc aaacgagtag aatagggggg
     4621 gaccaaaaat gaaagaacta ttttcgacat acaagctccc tatcatcttt ggggtacttg
     4681 gattgatatt agccgtttta tggatcaccg tgggcttttt caagacctta ttgatcttga
     4741 tcatggcagc gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa atcttagatc
     4801 actattttaa atcttaggag gaaacatcca tgaataacaa taacaaaaat aataacaata
     4861 aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc aacgacacca aaccaatcaa
     4921 atggtgtgaa cgatgaaaag aacaaaacag caccagtatc tgcacatcca gatccagtag
     4981 tccctaacgt tcaacaagga gtagaagaga agaaacatga tgcagaaaag catgcacctc
     5041 atcatccagg aactccagaa tctaaagttc cagaacaaca tgatgcaatc gatccttcta
     5101 aacctgtgga agagcaaaaa catccttctg accatgcgaa agcacaagaa aaacttagtt
     5161 ctgaccacac aaacggacaa ggaaaacttg gttctgatca tacaaataac caaggaaagc
     5221 atgattctga acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc
     5281 aaaaaatcat cggaatcgca attgaacaag tagacggttt attatctgca aacggtggtt
     5341 tcttctctaa tgtggcagga aaacttgtga atacagataa tgtaacggct gggatcgaaa
     5401 cagaagtcgg taaaaaacaa gtagcagttg atatggatgt cattgtggaa tatggcaaag
     5461 atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg caaagaagta aaaaatatga
     5521 cacatttaga agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca aaagaagaat
     5581 ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg
     5641 aatttgcttc gaatcaaaca gataaagtaa aatcagcagc tggaagcggc gcacaaaaag
     5701 taaaagaaaa tacagaacca cgggttcagt aataaggaag taaaacatta ggaggaacga
     5761 aacatgggat ttatttggtc acttatcgtc ggaggaatta tcggagcaat tgcaggagca
     5821 atcacgaata aaggttcttc gatggggatt attgcaaatg tcgtagcagg tttagttgga
     5881 tcagcacttg gtcaagcatt attaggttct tggggaccaa gtattgcagg aatggcaatc
     5941 gtgccatcat tgattggtgc catcatttta gtactaatcg tatcattctt tgctcgaaaa
     6001 ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa
     6061 cagccaaaca aaaatagctt ctcatatttt tcatttggtt gggcatatta tcgaagatgc
     6121 aagcttttga acactgttta tacggaataa gaggctgtga caagactttt gtcacagcct
     6181 cttatttatg cgggcaaatt cccaattaga tttttaaaag tttttcctta aatgtgtctt
     6241 tattgataca gagaagcttc tttaataaaa caatgcctcc ctaaattttc ttgagatagg
     6301 aaaggtgaac atacaaaata atacgaccac tttgaaaacc cagatgatcc acaaatggat
     6361 cgtatgatac tgatgaagta aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac
     6421 aaggaagaaa taatatctct tttttactct tgtctgtttt agccaataat agctcgtaaa
     6481 gtaatctaga caaacaaata gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg
     6541 ttgctctgct agacgattga cgagcaggaa aaatgttggt aatggaatat agatcacgag
     6601 taatgaaaga aaaaagtaaa caataaatgg catactattc ctcctaattg tgtgagttcg
     6661 ccctcatttt aaaataacga tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta
     6721 tctaatgtat ttttaaataa ataatcatta tta
//
LOCUS       DACDKL010000127         6746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDKL010000127 DACDKL010000000
VERSION     DACDKL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.127"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            87..1640
                     /locus_tag="IV329_002043"
     CDS             87..1640
                     /locus_tag="IV329_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8941211.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2021..3805)
                     /locus_tag="IV329_002044"
     CDS             complement(2021..3805)
                     /locus_tag="IV329_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8941212.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3820..5541)
                     /locus_tag="IV329_002045"
     CDS             complement(3820..5541)
                     /locus_tag="IV329_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8941213.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5641..6402
                     /locus_tag="IV329_002046"
     CDS             5641..6402
                     /locus_tag="IV329_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8941214.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2138 a         1202 c         1320 g         2086 t
ORIGIN      
        1 caaattcata aattttttat tagtgggact agtgattgta tggattttga cgcataatga
       61 atgaagtatg agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat
      121 cagctaaaca aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc
      181 ttttaagctt agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc
      241 gccatatgag tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc
      301 acttttcgtg gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta
      361 tttggctccg ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac
      421 aacagttttt acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga
      481 cttcgacgag tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac
      541 ttgatgattt acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc
      601 atattttttt actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat
      661 acctgacaac taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa
      721 caatctatta tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc
      781 acatgacaat gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag
      841 ctgatcctaa gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata
      901 cagagacgca ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga
      961 ttgattcgca atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag
     1021 gttattggat ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt
     1081 ctggggatga tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta
     1141 ttatcaccca atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg
     1201 gtctatttat ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta
     1261 ctatggatgg cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac
     1321 aaaatgtcag cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt
     1381 atacaaacaa cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc
     1441 agacaggggg gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata
     1501 cgttagagac aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg
     1561 cctgcctctt ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc
     1621 aagtagaaca aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac
     1681 aagaaacaaa ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg
     1741 agttgacaag agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg
     1801 tgtcttattg ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac
     1861 aagtcttttg tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag
     1921 tattattctt tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg
     1981 actaatcata catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac
     2041 tattataaag actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt
     2101 cgacgatatc gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac
     2161 ggtgtgcaat cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca
     2221 atgcttctgt tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat
     2281 tcgctagaat gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt
     2341 tttcgctaga aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt
     2401 gtgcgatttc agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga
     2461 tattatcagc taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac
     2521 gacgtagttc tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt
     2581 tattcagttc ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg
     2641 gaccaacgat cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata
     2701 ttttatctga tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt
     2761 ctaaaagtct tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata
     2821 tcctatcgat tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa
     2881 ttggttcaga aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac
     2941 cacctgatag aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa
     3001 tgaaacgaat ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt
     3061 attgcttttt atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt
     3121 tgaccactag attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat
     3181 actgattctg ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac
     3241 tgccaacgat cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca
     3301 gaccagcagc agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca
     3361 cttgagaaag ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat
     3421 tgtcaaaaaa cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca
     3481 atgtgatctt ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc
     3541 ttaagactga acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac
     3601 tgaaagaaag ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga
     3661 ttcccatgat caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca
     3721 atccgtaaaa atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg
     3781 tgttcaacgt ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc
     3841 aaactcctga taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat
     3901 ttttccttca tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc
     3961 aacaatcacg agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc
     4021 tgttttatag tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat
     4081 caacgctcga gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg
     4141 agcaacaaag ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat
     4201 cttgatcgct ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct
     4261 taaaattgtc ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat
     4321 ctgttcttga gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg
     4381 gtcactaagt ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac
     4441 gatcgctgtt gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga
     4501 gatttcagta tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt
     4561 tggttcagct actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc
     4621 ttctatccgt cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat
     4681 catcaagtaa gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg
     4741 taatgctcct tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc
     4801 catgagcaaa aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg
     4861 attagcatag tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa
     4921 ggcacggatc atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat
     4981 ttttggctgg atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca
     5041 tacataaaaa acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat
     5101 catgagaatg gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac
     5161 aatcatttgc tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact
     5221 aaaacgactg atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt
     5281 ttggaatcca aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat
     5341 tgttcctaga aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc
     5401 gccacttgct atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc
     5461 ggcgatttct aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa
     5521 gaatgttttt aagatagaca tattattcct ccttttttcc tgatactttt cgattctata
     5581 ccttcaagta acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa
     5641 atgaaaaaaa tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg
     5701 atccgctatt gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat
     5761 cgattgtttg atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta
     5821 gatataccga tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag
     5881 acacttgatg aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa
     5941 aaagagataa gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa
     6001 tttcctgtag aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg
     6061 gaaatcaaaa aatttctacc taactattca agctttggga tgatgagtat gccagcttcc
     6121 agttcacaga cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa
     6181 gacgtgattt gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag
     6241 atgaaccatg cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga
     6301 tggaaaacag gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc
     6361 agaactgaat attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat
     6421 aactcgaaaa tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt
     6481 taatgtgggg gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt
     6541 ctttaaaaat ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa
     6601 aaagtatgga aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa
     6661 tgagaagaaa aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa
     6721 ataggtgcat aaaagcatta cactta
//
LOCUS       DACDKL010000128         6716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDKL010000128 DACDKL010000000
VERSION     DACDKL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.128"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..923
                     /locus_tag="IV329_002047"
     CDS             51..923
                     /locus_tag="IV329_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8941215.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1265..2137
                     /locus_tag="IV329_002048"
     CDS             1265..2137
                     /locus_tag="IV329_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941216.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            2134..4092
                     /locus_tag="IV329_002049"
     CDS             2134..4092
                     /locus_tag="IV329_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8941217.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(4424..5275)
                     /locus_tag="IV329_002050"
     CDS             complement(4424..5275)
                     /locus_tag="IV329_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8941218.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            5398..5865
                     /locus_tag="IV329_002051"
     CDS             5398..5865
                     /locus_tag="IV329_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8941219.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(6128..6610)
                     /locus_tag="IV329_002052"
     CDS             complement(6128..6610)
                     /locus_tag="IV329_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8941220.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
BASE COUNT         2047 a         1371 c         1254 g         2044 t
ORIGIN      
        1 ccttttaact atacttagtg gtatgctttt ttaaaaacag gagggaaaaa atggatattc
       61 gtatactgac gtatttttta gcagtagcta gagaaaaaaa tatcagccga gcagcaaaat
      121 ctctgcatat cacgcaacct actctctcaa aacaactgaa agatttagaa gaagaacttg
      181 gtgtgacact tttcactcga ggctcccgag agatacagtt gacggattct ggtcgatatc
      241 tttattcaaa aggaaaagaa atcttgtcgt tggtagaaaa aacggcgaac aatttaacaa
      301 atgacgcaat catctctgga gaaatctaca ttggcggagg cgaaactaaa gcaatctctt
      361 tcatttctga tagccttcat tcgttgatca aggcgcatcc tgatatccag tttcatttat
      421 atagcgggaa tgcagatgat atcatagaca aactggatag cggattactt gattttggac
      481 tcgtaatcga accgacaaac aaacaaaaat accagtcatt tcgactgcca gcccaagatc
      541 gttggggact tttgatacat cggaatcatc ctttagcaaa gcaaacagtc atccatccgc
      601 aggaactgaa aactgttcct ttgatcattt cccggcaaac gtcagtggac agtcaactag
      661 ctgaatggct aggtttcagc atagatcagt tatcgatcgt cggaacatat aacttattgt
      721 acaatgcttc tttgatggtc gataacggga cttttggtgc attagctcta gatgggatca
      781 tcaatactta tgggacggaa aaagtattca tcccactcga tccgccatta caagcttcac
      841 tgaatctcat ttggaaaaaa gaccagcctc tttctcctgc tgctcgtaaa tttttagatg
      901 tcctaacgga gaaatacagt taagtagagt ttttttcctt cgctttcagc tttttaacaa
      961 cgaataagtt aagcaaaaga tccaatgatt aaataacata agtttcttaa tttgttcctt
     1021 ccttttattt ctgataattc cagtaaatta ctataattat aaaaaaagaa ttgacaaaac
     1081 ataactgtct atataaaatt gattacgtta acaataattc gtttcatgtg actagatcat
     1141 actaggcatg aaaaatctct gaggaacatg gtttccctag gaggtttttc gtgtctattt
     1201 ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc
     1261 gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta ttggtagatg acggtggaca
     1321 ggaaaaagta gctattggaa aagggattgg atttgacaga aaaagaaatg atttgatttt
     1381 tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa
     1441 tttacttaat cagattgatg aacgcttttt atttgcagca gaaacgatca ttgatcatgc
     1501 ggaaactgtt ttgatggaaa agttgaatga acatgtattg atcgctttga ccgaccacat
     1561 tgctttttct gcggagaaca ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga
     1621 aatcgaagtg ctttacagtg atgaattcag tattgctcaa tgggcagtag agtatctgac
     1681 aaaagagctt gggattccat atacttatga cgaagcagga tatatcgcaa tccatattca
     1741 cagcggacga agcggtcgga acaataacca tcgcagtatc cgtgaagtga cgattgtatc
     1801 tgacatcatc catttagtgg aatcagaatt ggaaacagat atccattcag aaccattttc
     1861 actgaattat tcaagactgg tgaatcattt acgactgctt ctacaacgaa cccatgcgca
     1921 acaatatgct gttttagaca cggaaatcgt agaaatggtc aaacgaaaat acccagaaag
     1981 ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac
     2041 gaaagaagaa ctgggttact tggccatcca tatcgagcgt ttaagaagcg caatcgtaca
     2101 aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag catatctaca acgaatggga
     2161 cgttcccttc aacttccagt tgcggtctta cctgctgctg ctttactagt agggatcgga
     2221 aactggtggg catcttatag taacgacatt gtcgctcatt ttttacaagc tggcggtaac
     2281 gcagttttag gtcaattacc tttgctgttt gccgttgggt tagcactagg gatgtcagtg
     2341 gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt
     2401 ttaaaaactg attcagtagc tactttattg aatatcaaac cagaatcagt tgatccatct
     2461 tttgctcaga tcagtaacgt atttattggt attattgccg gattgattgc ggcagctctt
     2521 tataatcgtt tccacgaagc aaaattaccg atggctttat ctttctttag cggcaagcgt
     2581 ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca gtgcagtgct actgctcgta
     2641 tggcctccgg tttactctgc tttagtttct tttgctaaat tcattttgag tttaggctgg
     2701 ggtggtgcag gtctttatgg cttcttcaac cgtttactga tccctacagg attgcatcaa
     2761 gcaatgaact ccgtgttctg gtttgacgta gcaggaatca atgatattgg taaatttttg
     2821 gcttctgaag gaacaaaagg cgtaacagga atgtatcaag ctggattttt cccaatcatg
     2881 atgttcgggc ttccagcagg agcatatgct atttacagaa atgcacgtcc agaaaacaaa
     2941 gcaaaaacag cttccttgat gttagcagca gcttttgctt cattctttac tggtgtaaca
     3001 gaaccacttg aattctcatt tatgttcgtt gcttggccat tatatgtctt gcatgcaatt
     3061 ttcactggga tttctttagc agtgagtgca ttcttccatt ggacagcagg atttgccttt
     3121 agtgccggat ttgtcgatta cttcctatct ttgaaaaatc cgattgcgaa tcagccattg
     3181 atgttgatcg tccaaggatt agtaacagca gtgatttact actttggctt taactttgct
     3241 atcaaaaaat tccatctgat gactcctggc cgcgaggaag cagatctttc agatgacgat
     3301 actgcaacga ctaattcaga caataaatat gcggcacaag caagcaaaat ctacgctgca
     3361 ttaggcggag ccgacaatgt aacttcgatc gataactgta ctacacgcct tcgtttgcaa
     3421 gtcaaagata caggcctgat cgatcaaaat aaaatcaaag ccactggcgt acctggtatg
     3481 aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta cagaagtaca attcgttgca
     3541 gatgagatgg caaaactaca tggtggtgct gcaacacgac ctgcacaaac aaacaccgtg
     3601 gttaaaacag aaacatttgc cggtggaacg atcgaacaag atatttacgc tatcgcaaat
     3661 gggaaactga ttccaatcac tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt
     3721 gacggctatg ctgtattacc agaaaatgga gaaatcttct ctccaatcgc tggtacgatc
     3781 acaaacattt tcccaacgaa acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc
     3841 ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg aaccattcac tttatatgta
     3901 aaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt tagttgatct tgccgctatt
     3961 caatctgcag gcaaaaacac ggatatggtc gttgtattca caaatggcga caaagtccaa
     4021 agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc
     4081 aacaaagcct gagccatcgt cttccccaga cttttttatc gataaacaaa caggagctga
     4141 gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac ggcgttctcc tgccaactcc
     4201 gcggtaataa gtccagccaa acgtaacata tgaagagcta ttttttattt gactcttctt
     4261 attacttgat gcagaacagc ttttttacaa cctctgataa acggtgttca aaaaccagtt
     4321 tatcggtatg aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
     4381 tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata
     4441 tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat
     4501 cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag
     4561 ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt
     4621 caataatttc taacttttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt
     4681 cagttagatt gccggataat tctaagattt ctggagagtc actttctact agtacttttg
     4741 ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt cctgcagcat
     4801 aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc
     4861 aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt
     4921 ggtgatctgg cgctaataca ctgatcgagt gatcggcatc tgcaaaactt gtataagcga
     4981 tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat
     5041 ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg
     5101 agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc
     5161 ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga
     5221 attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg
     5281 ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt
     5341 tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg
     5401 aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa
     5461 gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat
     5521 gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa
     5581 ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc
     5641 atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt
     5701 ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa
     5761 gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa
     5821 gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc
     5881 ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc
     5941 cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct
     6001 tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg
     6061 aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc
     6121 tatattatca tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg
     6181 taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc
     6241 ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg
     6301 ctaaagcgat tacaacaaga tacgttcctt ttgggaatgg gatgattcgg gtagtgtttg
     6361 gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt
     6421 tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac
     6481 cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc
     6541 tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat
     6601 actttgccat ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg
     6661 gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac tatttt
//
LOCUS       DACDKL010000129         6716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDKL010000129 DACDKL010000000
VERSION     DACDKL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.129"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..765)
                     /locus_tag="IV329_002053"
     CDS             complement(<1..765)
                     /locus_tag="IV329_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8941221.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDS"
     gene            983..2050
                     /gene="ulaG"
                     /locus_tag="IV329_002054"
     CDS             983..2050
                     /gene="ulaG"
                     /locus_tag="IV329_002054"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8941222.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            2068..2529
                     /locus_tag="IV329_002055"
     CDS             2068..2529
                     /locus_tag="IV329_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8941223.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            2549..4033
                     /locus_tag="IV329_002056"
     CDS             2549..4033
                     /locus_tag="IV329_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8941224.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            4076..4375
                     /locus_tag="IV329_002057"
     CDS             4076..4375
                     /locus_tag="IV329_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8941225.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            4390..5028
                     /locus_tag="IV329_002058"
     CDS             4390..5028
                     /locus_tag="IV329_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8941226.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            5033..5893
                     /locus_tag="IV329_002059"
     CDS             5033..5893
                     /locus_tag="IV329_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8941227.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            5883..6593
                     /locus_tag="IV329_002060"
     CDS             5883..6593
                     /locus_tag="IV329_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8941228.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
BASE COUNT         2150 a         1098 c         1469 g         1999 t
ORIGIN      
        1 tgaatctact tgaatggttt ttatccccgt atttctgatc tcctctaata ctttttcaga
       61 acaaaattta tcagtgatca aataatcaaa gactgaaata tcagcaatct tgaactttga
      121 tgtcagtccg atttttcgat gatcagcgac taaaatctta catttgattg tcttgttgac
      181 catcatttcg ttgatcgttg attcatttaa tatctttgtt gtgaccccat gtactacatc
      241 gactccgcta cagccaatga tacaaatgtc tgcattgact gaagaaatcg tatttaatgc
      301 gatgtcgccc actagtgctt cttttggaaa gcgcatctct ccaccagtta aaatataact
      361 acaattatct ttgtgcggag agtaattgat tttcacgttg tttgtaatga ctgtcaaatg
      421 gttagattga agaaaatcca gtgttttcaa cgcagtcgag ctagaattga taaacagcgt
      481 atcataatcg ttaatgtatt tactagcttc tctggcaatc gcttgtttaa tcctttcagg
      541 accagattga tcaaaatcag tctggttttt gtaattataa gaacaataac cgtaattgcg
      601 tatgatctcg tttttttctt ctaataaatt taaatctctt cgtatcgtac taatagatac
      661 atcaagggat tcagctaatt cgaaagttga tagttgttca gtctgctgaa gcagttccaa
      721 aatttctttt tgacgttttt caatattgac aagtgagttt ttcatgactt ttgcctcctg
      781 agggacatct tgatccatgg atttatttga ctagttgttc atttaaacat tcatatctgt
      841 ctttattata ctgaaacaat cattgattac tgaaagaaaa tcatataaaa aacaaacggt
      901 catataaaag ggttttcatg gatgctgata cacattaata tttacttgta agttggaaca
      961 acgatatggg gggagcaagc aaatgagtaa aactgtcaat gaagtaacaa aagaaaagtg
     1021 gttagtagaa acatttcctg agtggggcac atacttgaat gaagaaatcg aaacagaagt
     1081 agtggatcct ggaacttttg ctatgtggtg gctgggctgt acgggaattt ggctgaaaag
     1141 tcatgaagga acgaatattt tatgtgatct atggtgtggg acgggaaaac gtagtcatgg
     1201 gaatggcatg atgaaaaaag gccatcagat gatgcgtatg agtggcgttc aaaatatgca
     1261 gcctaattta cgtactcagc ctttcgtaat cgatcctttt gcgataaaaa atgtagacgc
     1321 tttaatcgtt acacatatcc attccgatca tctagatatc catacagccg cagcagttca
     1381 tcaaaattgt cctgaagcaa cattcattgg gccaaaagaa gtggttcgca cgtggcaaaa
     1441 atggggaatt cctgaagaaa aaacgattac agtaaagcca ggtgatgcag tcaaaatcaa
     1501 agacatcaat gtagtagctt tagaagcgtt tgatcggaca gcgttggtca cttgtgaaga
     1561 tccagaagag gtattaaaag gaaaaatgcc tcaggagatg gatcaaattg cagtaaacta
     1621 tttattcgaa acatcaggag ggaatcttta tcatgcagga gattcacatt actccaattt
     1681 atttgccaaa catggtaatg aacacaaaat cgatgtctgt ttaggggcct atggtgaaaa
     1741 cccacgaggg ataacagaca aagttacttc tgttgatatg ctgcgcatgg cggagtcatt
     1801 gaatgcaaaa gtagtgattc ctgtccacta cgatatttgg tctaatttta tggctgatcc
     1861 aaaagagatc actgaaattt ggaaattcaa aaaggatcgc ctgaagtatg gatttaaacc
     1921 ttatatctgg caagtaggcg ggaaattcat ctatccgaca aacaaagatg atcttgagtt
     1981 taatttttat cgaggatttg aagatgtctt ctcgattgaa aatgatacac cgtttccgtc
     2041 gtttttataa gaaagaaggg gagtttcttg ctgaaatact tttttgacca tcaattgatt
     2101 cattacccag ataaagaaat cacggattgg cgtgaagcta tcgccgaaag ctgtcttgta
     2161 ttgatcgata aggaaattat tgatgaaacg tatgtggagg agatcatcca ttgtgtagaa
     2221 aaatacggtc cttatattgt gatcgcacct caagtagcaa tgccgcattc ttcagaaaaa
     2281 agtgatggtg tttttggaac agctatttca tttaccaagt tgaaacagcc aatccgtttt
     2341 gaaagtgaag gggagaatga ggagaaaacg gctagtttgt tttttacact cgcagcaaaa
     2401 gatccagaag aacatctaga aaatatccaa aaattatccg aattattaat gacagatgga
     2461 ttggttgagc gtctcatctc tacgaactcc atcgatgatt accaagttgt gatggcagaa
     2521 tataattaat tgatagagga gaaaaaagat gggggaattt ttgttaagca tttggacgta
     2581 ttttgcaaca aatatcttga cgcagccagc atacctgatt ggttttatcg tgttattagg
     2641 ctatattttg ctgaaacgcc cgcttcatga atgcatcgct ggattcttaa aggcaacggt
     2701 cggttacttt attttgactg ttggctctgg tggtttggtc aataattttc ggccaatctt
     2761 ggttggacta aaggaacggt tcaatttgga tgccatggtt acagatcctt attttggaca
     2821 aaatgctgtg gatgctggat taatggagac atttggccgt acctttggag atgtgatgat
     2881 tttattactg attgcatttg ttttaaatat cttattagtc cgttttcaga aatatacgaa
     2941 actgcgttcc gttttcacta ccggaaacgt tcaggttcaa caagcagcta ccgctttttg
     3001 gattttactg ttttgctttc ctgatctagg taggatacaa gcattgattt ttatgggact
     3061 gattttaggt tgttattgga cagtagcttc taacttaaca gtaggaatca cgcaggaatt
     3121 gacagaaggt gccggatttg cagtggcaca tcagcaaatg ttcggaatct ttatatttgc
     3181 aaaattagct gagtggatga aaaagcggga tgaaaagaaa aatagatcaa tcaagcaaga
     3241 caaaaaaatt gaagatatca aattaccagg attcttgtcg attttcaatg agaatatggt
     3301 tgctacttcg ttactcatgc tatttttctt tgggatcatt ctgattgtac taggcaaaga
     3361 ttatttgatt caagcgcaat tcatgcaaga agggcagagt ttcttattct acattatgac
     3421 gacttcttta aattttgcgg tatatctggc gatcttgcaa ttaggtgttc ggacattcgt
     3481 cgatgaacta actcaatctt tccaaggaat ctcaaacacg attttaccag gagctgttcc
     3541 aggaattgac gtagctgcta cttttggctt tggttcgcca aatgcggtaa cgattgggtt
     3601 cttgtttggt gctttgggac agtttttgat gattggttta ctgattttat tgaaatcacc
     3661 aacgatcgtc atagccggtt tcattccgtt gttctttgat aatgccgtta tagcagttta
     3721 tgccaataac cgaggtggat tcaaagccgc ttgcctgttc ccgtttcttt caggaatcat
     3781 acaagtaggc ggttcagcat tattcgctac atggattggg atgtcaacat acggtggtta
     3841 tatcgggatg ttcgactggg caacagcttg gccattgttt acagtaatca tgaaagtcct
     3901 aggttatgct ggaattgccg tagttgtcat tggtttattg gctattccgc agttacaata
     3961 tcggaaaaat cctgatggtt attttttgat tgtagaagac tatgacacat atgcagaaaa
     4021 gcagagagta taaaagaaaa tctgattaga caacactcat aatggaggga ttaagatgag
     4081 aattttagta tcgtgtgcaa atggatcagg aacaagcttg atgatgatga gaagtgtgga
     4141 aaaagcaatg aaagaactaa acgtaccaat taccaaaatc catcattgtg ccatttcaga
     4201 gggaaaaagt tcagctggcc aatacgatgt agtttttacg ccagtcaatt ttttgaatat
     4261 gtttactcag gcagagaaaa aaggagttac agttatcggt atcaaaaatg tgatgtcagt
     4321 caaagaaatc agtgaaaaat ttgctgaaac aaagtggtac caaaaaaaca agtaaaggaa
     4381 ggaactaaga tgagcaggcc gaatttgcaa attgcgttag atcacaatga cttagaacat
     4441 gcattaggag atgttatgaa agttggtgat gtagtagata ttattgaagt gggcacaatt
     4501 ctttgcttac aggaaggaca aaaagcgatt cgctgtattc gcagtatgtt cccagataaa
     4561 aaactagtcg ctgatacgaa atgtgcagac gctggaggaa cagtggcgag taatgttgca
     4621 aaagcaggag cagactggat gacagtcatt tgttgtgcaa cgattccaac tatggaggca
     4681 gcacaaaaag aaataggtga attgcaagta gaactctacg gcaattggac ttttgaacaa
     4741 gccatggatt ggcataacat cggtatccgg caggtaatct accatcaaag ccgtgatgcg
     4801 ctgttatctg gtgaaacttg gggtgaaaaa gatttatcga agatcaaaaa actgattgaa
     4861 ctaggcttta atgtttctgt aactggtgga ttaaatccac acacattaca cttatttgaa
     4921 gggatcgatg tgtatacctt tatcactggt cgtggtatta cagcagcaaa tgatccgatg
     4981 aaggccgcac aaaacttcaa agatgagatc attcgtatct gggggtaagg aaatggctca
     5041 aattgggatt tatgaaaaag cgttacccaa agatatttca tggaaagaac gtttttccct
     5101 tgtaaaagaa atggacttcg attttatcga gatgtcgatt gatgagacgg atgagcggtt
     5161 agcacgatta gattggtctg aagaaaagat agctgagctg agagaagaga tgttcagctc
     5221 cggtgtacgt atccattcga tttgtttaag tgcgcatcgg cgatttccgt ttggttctgc
     5281 tgatccagaa aagaaaaaag cagcgaaaca actaatgaaa aaagcaatcc atttggcgca
     5341 taatctaagc gtcaaagtaa tccaaatagc tggatacgat gtttattatg aagagaaatc
     5401 aatgaattcc agagaagact ttatcgaagg aataaaagaa agcgtaaagg aagcttctaa
     5461 atatggcatc attctttccg tggaaatcat ggatgatcca tttatgaact ccatttcaaa
     5521 atttttagaa atcaaagaac agattcattc accttttcta caagtatatc cagacctagg
     5581 caatttgtcc gcatggccgg aaaataatcc ggctcgagaa cttgagaaag ggatcgatta
     5641 tatcactgct atccacttaa aagatactta ccctgtaaca aaagaatatt caggccagtt
     5701 tcgtgatgtt ccttttggta gcggttgtgt tgattttctt ggattattaa gacatttgaa
     5761 acgtctagat tatgatggaa catttctgat tgaaatgtgg agtgaaaaaa gccaagactt
     5821 tcgaagagaa atccagcagg caaaagacta cttaagttct aaattgaagg aggctggcta
     5881 tgatgtcttg tgaagaaaaa attgcagcaa tgaagcaacg tgtccttgaa gcaaatctgg
     5941 cattgccgcg atacggatta gtcaaattga cttggggcaa tgtgagtgaa gtggaccgtg
     6001 agctaggagt catcgtcatt aagccaagcg gtgtcagata tgaatgcatg caagcagatc
     6061 aaatggtggt aacagatttg tcgggaaata taatagaaga agatagctta aaaccatctt
     6121 cagatctccc cacacatgtt gttttgtatc aaacttttga agatataact gctattactc
     6181 atactcattc cacgcactcc gtcatgtggg cgcaagcagg acgcgatttg ccagcttatg
     6241 gcacgactca tgctgatgct ttttatggta aggttccttg tacacgccaa ttaacgaaag
     6301 aagaagtaag agaagcttac gaagttcata cagggaatgt gattgtcgag acgtttaagg
     6361 aaagaaaatt agatccaaac gaagtccctg gagtacttgt ctatggacat ggaccgttca
     6421 catgggggga ttcacctatg aaagcagtag aaaacagttt gatcttagat gaaatttgtc
     6481 tgatggccaa agaaaacgag ctgatcaacc ccaatatatg tgaaatcccg caatatttac
     6541 tagataaaca ttattttcgc aaacatggaa agtatgcata ctacgggcaa tagagatgct
     6601 agggtaaggg tagaaaatta aaagataaca cttgatagag agaaacagag gttgtgaaga
     6661 aaatttatca caaccttttt atttatcaaa aagtatgcat tctaaaagga aagagg
//
LOCUS       DACDKL010000130         6674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDKL010000130 DACDKL010000000
VERSION     DACDKL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.130"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..811
                     /gene="sufC"
                     /locus_tag="IV329_002061"
     CDS             38..811
                     /gene="sufC"
                     /locus_tag="IV329_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8941229.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            824..2110
                     /gene="sufD"
                     /locus_tag="IV329_002062"
     CDS             824..2110
                     /gene="sufD"
                     /locus_tag="IV329_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8941230.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2107..3342
                     /locus_tag="IV329_002063"
     CDS             2107..3342
                     /locus_tag="IV329_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8941231.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3329..3799
                     /locus_tag="IV329_002064"
     CDS             3329..3799
                     /locus_tag="IV329_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8941232.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3804..5195
                     /gene="sufB"
                     /locus_tag="IV329_002065"
     CDS             3804..5195
                     /gene="sufB"
                     /locus_tag="IV329_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8941233.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5290..6432)
                     /locus_tag="IV329_002066"
     CDS             complement(5290..6432)
                     /locus_tag="IV329_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8941234.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(6471..>6674)
                     /locus_tag="IV329_002067"
     CDS             complement(6471..>6674)
                     /locus_tag="IV329_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941235.1"
                     /translation="NILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNINK
                     LEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT         2103 a         1309 c         1421 g         1841 t
ORIGIN      
        1 atgagtgaga acgaaagaga atttgggggt ataatgcatg tctgtcttag aaataaaaaa
       61 tctacatgtg tcgatcgagg aaaaagaaat ccttaaaggc gtaaacctaa caatgaaaac
      121 cggagaaatc catgccatca tgggacctaa cggcacagga aaatctacct tatctgccgc
      181 tatcatgggc aatccaaact atgaagtaac agaaggcgaa attttgtttg acgggaaaaa
      241 tgttttagac ttggaagtag atgaacgtgc gcgacttggc ttatttttag ctatgcaata
      301 tccaagtgag ataccaggta tcacgaatgc ggaattcatg cgtgcagcga tcaatgcgaa
      361 acgtgatgaa aacgacaaaa tttctgttat ggcatttttg aaaaagttag atgaaaaaat
      421 ggctttacta aatatgccag aagaaatggc tgaacgttat ctgaacgaag gtttttctgg
      481 cggagaaaag aaacggaatg agatcctgca attgttgatg ttagaaccta catttgcaat
      541 tttagacgaa atcgattcag gtctggatat cgatgctttg aaagtcgttg ctaaaggagt
      601 aaatgaaatg cgcggagaca atttcggtgc attgatcatc acccactatc aacgattatt
      661 gaactatatc acacctgatg tcgttcacat catgatggaa ggccgagtag tcatgacagg
      721 gaatgctgac ctagctaaac gtctggaagc agaaggctac gctggtatca gtaaagaatt
      781 aggaatcgac tataaagaag aagaagcgta agggggagca agaatgaaag aaaaaaactg
      841 gctcgattat cttgacgcgg taaatgattt ttctttaagt aaaggcgaac ctgattggat
      901 gagaaccttc cgtcaagacg cactagcgaa agcagatgaa ctgccattgc cgcatatcga
      961 tcgggtgaaa ttccatcgct ggtcgttatt tgatgtgaaa gaaacacaaa ccatcagtga
     1021 aacaggcacg attccggcat ttgatgcaat gaaagacaat cctgtgcttg tccaacaagg
     1081 ttcatggacg atttttgaac aactgcctgt ggaattagca gaaaaaggag tcatttttac
     1141 cgacttgttc actgcaatga tcgaatatcc agaactagtc caagaatatt atatgaaaaa
     1201 agcagtaaac atgaacgaag accaattgac tgcactccat gtagctttta tgaacagcgg
     1261 tattttcctg tatgtgccta aaaatgtcgt gatcgatgaa ccactcgagt ccttattcat
     1321 tcaagatggc gcatccgatg agcacttctt caagcatgtg ttgatcgtgg cagatgaaca
     1381 tagtgaattt tcttatctgg aacgtttcca gacaaccaaa gaacaagtag caaaatcatc
     1441 aggtaatatc atcgtggaag tcatcgctaa agctggttca aaaatcaaat attcagccgt
     1501 agatcaatta ggagaaaata ttacttctta tatgaatcgc cgtgggcaca ttttacgaga
     1561 tgcttctgtc gactgggcaa ttggtgtcat gaatgacggt catgtgatcg ctgactttga
     1621 ctcagattta gcaggagaag gtgcacacgc agaagtcaaa atcgtggcaa tcagctcggg
     1681 cagacaaatc caaggaatcg atacacgagt aaccaataaa gcccctcata cgatcgggca
     1741 tattttgcaa cacggagtga tccgagaaaa aggaacattg acctttaatg gtatcggcca
     1801 catcttgaaa ggggcaaaag gtgcagatgc ccaacaagaa agtcgtgtct tgatgctttc
     1861 tgataaagca cgtggtgatg cgaatcctat tcttttgatc gatgaaaacg aagtaactgc
     1921 tggacatgct gccagtgtag gacgagttga tccagaagaa atgtactatc tgatgagtag
     1981 aggattgcat aaagaagaag cagagcgctt agtgattcga ggattcctcg gttctgtctt
     2041 aacagcaatc cctgtagaac aagttcggaa agaattagtc gaagtaatcg aagggaagtt
     2101 gaatggatga tcgatgctaa aaagctacgc aatgattttc ctatcttatt tcaagacgtc
     2161 aatgatgaac ctttagttta tctggataat gcagcgacga cccaaaagcc aaaacaagta
     2221 ctccaggcat tagcgtacta ttacgaacac gataacgcca atgttcatcg cggtgtccat
     2281 acgttagcag aacgcgcgac ccatgattat gaacaagcaa gagaaaaagt acgggcgttc
     2341 atccatgcaa aagaaacagc agaagtgctg tttactcgtg gtacgacgac aagcttgaat
     2401 tgggtagcca gaaccttcgg cgaccgctat atcaaagcag gagacgagat agtgctctct
     2461 tacatggaac atcattctaa tgttatcccg tggcagcagt tggcaaaacg aacaggtgct
     2521 gtcttgaaat atatcgaact aacagaagaa ggcttcctgg atatggagga tgcagcgaaa
     2581 aagattacgg acaaaaccaa aatcgtctcc attgctcatg tatccaatgt cttaggtgtc
     2641 ttcaatcctg ttcaagaatt agctgaactt gtccatgaaa acggcggggt catcgtagta
     2701 gatggtgctc aatctgcccc gcacatggcc attgatgtcc aagcactaga ctgtgatttc
     2761 tttgctttca gcggacacaa aatgtgcgga ccaacaggta ttggtgtttt atatggtaaa
     2821 cgaaaatggt tagaagaaat ggaacctgtt gaatttggtg gcgaaatgat cgattttgtc
     2881 gaattatacg acagtacatg gaaagaacta ccttggaaat tcgaagcagg gacaccaaat
     2941 atcgccggcg ctatcgcatt aggccatgca atcgattatt tgcaaaaact cggtatggac
     3001 aatatccatc gatatgaaga agaactagcc gcttatgtgt tgccaaaatt acaagcaatt
     3061 gatggattga ctgtttatgg tccacaagac ccgaattttc ggacaggagt cgttgcattc
     3121 aatttggatg ggttacatcc ccatgatgtc gcgacagctt tagacatgga aggggtagcc
     3181 gtcagagcag gtcatcattg tgcgcaaccg ctattgaagt accttgaagt atcagcgact
     3241 gctcgcgcga gcttttattt ctacaacaca acgcaagacg ccgaccgttt agttgaagca
     3301 atcatggcaa caaaggagtt tttccaacat gggactatct aaattagaca atttataccg
     3361 tcaagtgatc ttggatcatt cttcccatcc gcatcatcgt ggaagcttga ctgactcgac
     3421 aagcaagatc gaattgaaca accccacatg tggagatgtg atcgaattgc aagtcgatgt
     3481 aaaagacgga atcatcaaaa atatcgcctt tgacggcagt ggctgctcca tcagtacggc
     3541 aagtgcctcg atgatgacag acgccgtcat cggaaaatca atagaagaag cagaacattt
     3601 aactgtagac tttctacagc ttgtccaagg aaaagaagtc aaagatcccg accaattagg
     3661 cgatgctgct atgcttggtg gtgtagctaa atttcccgct cggatcaaat gtgcgacact
     3721 tgcctggaaa gcactaggaa aagcaatgga aaacgatggt accgcagctt tccatcatta
     3781 ccacaaggaa gaggagtgag aacatgggcg taccagaatt agaagaatat aaattcgggt
     3841 ttcacgatga tgtcgaaccg gttttcagta caggagaagg cttgacagaa gaagtgatcc
     3901 gtgagatgtc aagaatcaaa ggcgaaccag aatggatgct tgaattccgt ctgaaatctc
     3961 tagaaacatt taacaaaatg ccaatgcaaa catggggccc cgacctttca gacatcgatt
     4021 tcgatgccat caaatattac caaaaaccaa gtgacaaacc cgctcgtgac tgggaagacg
     4081 taccagaaaa aatcaaagat acatttgaaa agattggtat accagaagct gaacgagctt
     4141 atctagccgg tgcttccgca caatatgaat cagaagtcgt gtaccataat atgaaagatg
     4201 aattcgaaaa gttaggaatc gtctttactg acactgactc tgctttgaaa gaataccctg
     4261 aattattcaa agagtacttt tcaaaacttg taccgccaac cgacaacaaa ttagcagcgc
     4321 tgaattcagc cgtatggtca ggtgggacct ttatttacgt gccaaaaggc gtcaaagtcg
     4381 atgttccatt acaaacgtac ttccggatca atgcggaaaa cacggggcaa ttcgaacgta
     4441 cgctgatcat tgttgacgaa ggagcaagca tccactatgt cgaaggttgt acggcaccta
     4501 cttattcaag taacagcctg catgccgcca tcgtagaaat ctttacaaga aaagatgcgt
     4561 attgccgtta cacaacgatc caaaactggt cagacaatgt gtacaactta gtaaccaaac
     4621 gagcaaaagc atacgaaggc gcaaccgttg aatggatcga cggaaaccta ggtgcaaaaa
     4681 caacgatgaa atacccaagt gtttacttag atggaaaagg tgcacgaggc accatgctct
     4741 ccatcgcctt tgcaggagca aaccaaatcc aagatacggg tgcaaaaatg atccacaatg
     4801 caccaaatac atctagctct atcgtctcta aatcaatttc aaaagacggc ggagaagtaa
     4861 actatcgagg acaagtaacc tttgccaaaa acagtgcggg ttcgatctct catatcgaat
     4921 gtgacacgat catcatggac gaatggtcga aatccgatac gatcccattc aatgagatcc
     4981 acaacagcca agtctcttta gaacacgaag ccaaagtctc aaaaatctca gaagaacaac
     5041 tctactatct gatgagccga ggcctgaccg aatctgaagc aactgaaatg atcgtcatgg
     5101 gctttgttga accattcacc aaagaacttc caatggaata tgcagtcgaa ctcaatcgat
     5161 tgatcagtta tgagatggaa ggcagtgttg ggtaaacctg ttataggagc tagaaccctt
     5221 gatatataag ggttctagct tttttgtttt gcctgtatac tagaaatgac cagattttga
     5281 ccagctcttt tactgatgag ccatcatatc agcaaagtct tgacctgttt tcttaggtgc
     5341 attatttgta atatgtgcat agatgtctaa tgtcacttta ctattttcat gccctaatcg
     5401 ttgttgaact accttgacag attctcctgc ttctaataat aaactagcat gtgtatgtct
     5461 aaacccatga ggtgtaatac gaggcaagtt atattttttc aaaatatatc ttaaccaatc
     5521 attaacagtg tttggaatgt atagagtgtt tttaactgtt gtaaaaacat gttgctcttt
     5581 agagctagta ttgtatccaa gttttaaata ctcttctttt tgctgtaatt tccagttgtt
     5641 gaggatagaa agagtctcag tatctaaaga gataactcgt ctagaagctc ttgttttcgg
     5701 agattgaatt ataactttac caaattcatc tacagcaaga gttttattga catgtagttc
     5761 tttattaaac gtgtctatgt ctttccactg taaagcgagt acttcactct ttctcatacc
     5821 tgtgtatgcc aataatctga agaaggcgta ttgtttcatg ttaccaaaat ctttaaaagc
     5881 atccagtaaa ttattaagct cctcttttga gtaaaattta cgatttttct catactcttt
     5941 ttttcgtgga agaagtgttt tttccatagg attattgtca atatattgca ttgcaacccc
     6001 gtagcgtaaa atttgtgcag tctgttttct catataatcg taactttcat atttactgtg
     6061 ccattcatct accacttttt gacaatatgg gacagagata ttcgatagtt taagcttacc
     6121 aaatgctgga agtatttgat tatttgcgta tcttactgag attgcgattg ttgaagcttt
     6181 aacactggta cgctgatgag ttatccattc attatacagg tcttcaaatg tcatatcagg
     6241 tgctttcttt ttaacaccgt ataatatttg atgtctgtac tcgtcataat ctttttgagc
     6301 ttcttgtttg ctactaaaac ctcttctcgt agtatttatt ttttcaccag ttcgtggatc
     6361 ggtggcaatg tgtcctttat acatccacgc cacttgaccg tttttctttt tgtactttga
     6421 tatttttgcc attaaatcat ctcctcttag tttacaagga gtacttacca ctagatggta
     6481 tcacctccct tcattttttt cttagtgtat tttttcccaa ttgatgaaat cgtttgttct
     6541 agcttgttta tattatcagt aatttctctc acagcttctt cgggataaga catgtctttc
     6601 aattgaatag gtagccattc atctaccatt ctttctagta gttcttctat actgctagtg
     6661 aggaaaagaa tgtt
//
LOCUS       DACDKL010000131         6666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDKL010000131 DACDKL010000000
VERSION     DACDKL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.131"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2671
                     /locus_tag="IV329_002068"
     CDS             <1..2671
                     /locus_tag="IV329_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8941236.1"
                     /translation="MNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTADSQ
                     AIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGS
                     ATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQN
                     FLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNI
                     DRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEG
                     DHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPL
                     PKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVI
                     TLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPI
                     FFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVL
                     GTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPT
                     TVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSN
                     CQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLS
                     SNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGA
                     LLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNS
                     DNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCA
                     EKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2853..3092
                     /locus_tag="IV329_002069"
     CDS             2853..3092
                     /locus_tag="IV329_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941237.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3108..4481
                     /locus_tag="IV329_002070"
     CDS             3108..4481
                     /locus_tag="IV329_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941238.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4485..6119
                     /locus_tag="IV329_002071"
     CDS             4485..6119
                     /locus_tag="IV329_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941239.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6282..6488
                     /locus_tag="IV329_002072"
     CDS             6282..6488
                     /locus_tag="IV329_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941240.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTG"
     gene            6557..>6666
                     /locus_tag="IV329_002073"
     CDS             6557..>6666
                     /locus_tag="IV329_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8941241.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         2346 a         1090 c         1335 g         1895 t
ORIGIN      
        1 aatgaatcac acttcaattc agaagcattt acaactgtta aatacactgg ggatttctta
       61 tgttaatttg agaacttttt ctacattaaa caagaaaacc gtttctcata ttttttctac
      121 tgcagattct caagcaatta ttaactattt agaaaaacag aaattgtctg aaggtacagt
      181 aaacataact tataatccta ttaaacaaga ggtgattaca caaaaaaagc attctgtgag
      241 agacaatgat attgaaaaaa ttcggtttgt atttattgat attgacccta aaaggaaaga
      301 gggaagtgca acagatagcg aaaagaaaaa ggcagaaaat gttatggaac aagtagaacg
      361 ttatttgaaa gaaaagagaa tagaatcatt tgtaaaagtt gatagtggga atggctacca
      421 tatttttctt cccataaatg aacaaccaaa taatcacgag acgattctta ctattcagaa
      481 ttttcttcaa ttacttcacc gaagatttgg agtagaagat gaagttgata ttgatacttc
      541 tgtttataat ccttcaagat tatgcaaatt agttggtaca ccagcggtca aaggaaagtc
      601 cacaaaagag cgtccgcatc gtttatctag tttcctttct attcccgata acatagatag
      661 aagtaattgt tttaaaattc ttgaacagat agttgaagaa aattccatga tgtcaacctc
      721 tagagtaaaa agccatccat cttttagaga agtgttacct tttattcaag cagactataa
      781 acaatggtta gctcactatc ctgaattaac ttataccata aaagagggcg atcataaagg
      841 agttacctta cttatatttg acgaatgtcc attaagaaaa cacacgaata attctaatgg
      901 ttcttgtttg tctgttacgg ctgataataa gattcgattt cattgtctgc atgcttcgga
      961 taaaaataaa gatattcaag actttgtaaa aaaatatccg ttgccaaaac ctgtaaatcc
     1021 ttctgtagaa tcattagaat ccaatcaaga atattatttt gaccagttta aactgaatca
     1081 agaaggagta tttttggtga aagaaggtca agaagaaaaa gttgcttgtc cgatgtttat
     1141 taagcaaata cgcaaaaata tcgaacaaaa agtcattaca cttgttcttg cctattatgt
     1201 cgatcattgt tgggatgaaa tagaaataga aggtggatgt ttacaaacta ataattttaa
     1261 acaattagca aaacatggct taatcattaa agctagaaga gaagctgaag tcgttgactt
     1321 tttacagatt caacgggaga atgctccaat cttttttgaa catgaatctc ttggctggaa
     1381 aaaagaaaat gaagcaatga tttttcagtt acaagattct tttggacgtg aaattccttc
     1441 tgtattaact aaaaactcat gctaccaatt aacaactaat ggtacagaag cggaatttga
     1501 cgcattggtt cataagtatg ttttaggaac gaacttagaa cttgcttggt cgattggtat
     1561 tgcttctatt gtgttaggct atttaaacgt tacgaaggct aatacttttt tgagtttact
     1621 aattaaccta cagggcaaga gcacaacggg taaaaccact acgttacact ttattgcctc
     1681 tttatttgga aatccaacta cggttcttag aaatatgaat gctaccaata aagccattat
     1741 aaagctagca tcaaacaatc agggaggtgt tccactaata ctagatgagt taggagcagc
     1801 aactcaaaat aactttagct cgttgatcta tcaattagct tcaggagaag aaaggctaag
     1861 attagattct aattgtcagc ttatgccaca agagactttt tctgttttga cccttacctc
     1921 ttctgaagag cgtttggaaa attatttcaa tgagaaatta actggtttga aagttcgata
     1981 tatcgaattt tctgatatca agtttacaaa ggatgcgaat cacgcagaga aagttaaaag
     2041 tctttcttct aatacttttg gttggggtat aaagttattt cttttgaaac tctttgaaca
     2101 agggattaca aaaattgaag caacttttgt cgaagctcaa aaaaaactat gggctgtttt
     2161 acctgaacat tctttgaagc aacgcattgg taataatcta gctttgattt atagtggagc
     2221 cctcctaatt caagatatac tgggctatcc tttacgatta gagcatatac agacggtgct
     2281 tattaattcg tacgaacagt taattgtaga tactagttta ggaagtgagc aaacggtatt
     2341 tgaccgagtg aaaacattgt tggtgatgaa tagccataaa tttttaacaa acagtgacaa
     2401 caaacatacg cccagtacga tttggggaaa agtatcaatt caaaaaaatg gggatattaa
     2461 ggtaaatatt tttcctatcc cgtttgagga cttgttaagg cgagaatttc aagtaaagga
     2521 tctaacctat ctttttaggc aattgcttga ttcgggtaat atgtgtgcag aaaaagggag
     2581 aagaacgaaa cgtattcaca tgaatcgtca agttcttcca acatacgaga ttctattacc
     2641 ttcttcactc aaatcgtatt ttcgattgta atttagaata aaccaaagta tgaaagtgag
     2701 gcaccatgtt atggaaatat ttcttattga tttagcaaaa caactgatta cttatctgta
     2761 tgatagcgag caaacccaaa cggaagtaaa tgaagcttga tctatgcttc atttacttca
     2821 ttcaatcaaa catgaaagaa aggaaaattc taatgactaa aaataaaatg acactaaaag
     2881 cagaggttct tctgtatatt caagaacatt tttcaaatca agcctttttt acaaaaccaa
     2941 tttacttgga tttcgaaata agaggggtat ctgctggatc tatcgggggt actcttcaag
     3001 cgttgaagaa cgaaggatat ttagaaaatc actttgtgca acgttcgttc aatagaagag
     3061 tcgtgaaaaa atggtattta gttcattcat agagagggga atcaagcatg cgtgtggctt
     3121 gttatgatcg aacgtctgtt tatgaagaag cgaaaattag taatgacgct caatatgatg
     3181 cagtgaaaga gtatgtttta gagaaatatg aacaaattcc ccaacgattt tcagacaaag
     3241 gagaatcagg ggaaaaagcg gagcgtccag cttttcgaca gcttagagct tgggtttgtg
     3301 cgtcagaaga agagggggtg atctatgttt ggcgttatga tcgattggca cgtcaagcca
     3361 aaatagcctt agaatttatt gagttgtgcc aacaacatca ggttcaagtg tattcgattt
     3421 ctgaacctct tcctgcttct ttctcacacg cgtcaacgaa taaactattt gtgcaattat
     3481 tgttcacttt tgcagaacaa caacgaaacg tcattattga gaatgttcgt gccggacttt
     3541 cctataaaaa gcaacaacgg caatatttgt cttctgcggt accatatggg tatcgtttag
     3601 tgaatgggaa aatccaacaa gaagtacacg aagcaaggat tgttcgctat atttttcaac
     3661 tttatttgac caaaaaatat ggctacaaaa agctttgtca acgattgacg caacaaaagc
     3721 ttttcttccg cgaacgtcct tttcagccct atcatattta tagtatccta aaaaatccat
     3781 tgtactatgg cgaaatcaaa ggaggatctt tggggaagta tctaggaacc tttgaaccga
     3841 ttctctcgaa agcaaccttt ttacaagccc aagagattcg ccaatcaagg tgtacagcta
     3901 aaaaagatac gtatctgtac ttattacgtc aaaaaattcg gtgtcccttc tgtggtcgtc
     3961 atctgagttc gaagtatcaa tggaatacaa agaagacgaa gacgcttcat tattatcatt
     4021 gtaccgaccg aagctgccaa ggaatttttc ttcgggcaga aagggttgag tcagaagtat
     4081 tggaaaagat acaggctttt tttcaatacg aaggcgtcta tcaagccttg attgcccaat
     4141 taacgcaaca attagccgaa aagcgaaaag atgaaaaacg caaagtaatc caacaaaaga
     4201 aacaagaagc acaatggatt aaggcatttg aagagggcaa ttgttcactg gaagaactca
     4261 agcaacaact gcaagctctt caaaagccga tgtctacttc ttcgtttctt tcactccaac
     4321 agtgtgaagc aaaagtgaaa gaatggcttc aactaaaaga cctctctagt cgacaattat
     4381 tatgtgacta tgtttgtcgg attgaggtaa caaaggataa aacagtttgt gggatttact
     4441 tccgacaact gacacaaaat attttagaaa ggaacgaatg atctatggaa atgatacaaa
     4501 ccaaacgagt cgcattgtat attcgcgtga gtacacaaga acaagcttta gaaggacata
     4561 gtattcaagc acaagaacaa aatggacgac agtttgccga acggatgggc tatgaagtgg
     4621 tagaagttta tgccgatgaa ggaatcagtg ggaaatccac aaaaaatcgg ttcgcctatc
     4681 aacgaatgat gaacgatgct cggaaccatc gttttgatct actagtgatt tggaaattga
     4741 cccgtctcgg acgcaatatg ttagatgtct tacaaacagt cgaggaatta ttgcgatatg
     4801 acgtaggatt acattcgatc tcggaacaat ttgacgtgac cacttcgaca ggtaaattga
     4861 tgttacaact ccttgggagt ttcggtgaat ttgaacgcaa ccaaattgcg gagaatgttc
     4921 aaatgtcaat gcaaagctta gtgaaagata aaaagcgtta tgcaggaggc cgacgtttag
     4981 gctatgtcag tggtattgac acgaatggtc aaaaacaatt ggtcattgaa ccagaagagg
     5041 cgaaaattgt tcgtctgatc tatgcaaaat atttaggcgg agcagggtat cgagcgattg
     5101 ccaatttttt gaatcgtcaa ggatatcaaa cagtcaaaaa gaatgctttt tctaccacgg
     5161 cagtcaaaga tatcttacgc aataaaattt atgcagggta tttagaatat gcgcgctaca
     5221 tcaattggga aaccaaacga cggaagggga agaatccgag accgatttta gtgaaaggaa
     5281 cccatgaacc gattatttcg gaagaagatt atcaaaaagt acaagaacgc cttgctttag
     5341 aaggaaaaca acccaaatgg aaccatacag gtgaaaatgt attgacaggc ttgttacgtt
     5401 gtccagaatg tggcgcaccg atggcagcga gtaatgtcac aaatacccta aaggatggca
     5461 caaagaaacg gattcgatat tattcttgtt cagtctttcg gaataaagga gcaagtgtgt
     5521 gtcatgccaa ttcgattcgt gccgaacaag cggaaacatt tgtcgccgac cgattaaagg
     5581 agatcgttca gctaccagaa gttcttcccc gattggtggc agcgttaaat gaagaaatag
     5641 tgcgacaaag ccaaccattg gaacaagaac tcgtggtgtt actggaacga aaggaagaat
     5701 tgaagactaa aattgaaaaa tgggaagctg ctttggaaga tagtcctgaa ttgtttccga
     5761 tgttaaaaga ccgcttagac gaattgactg aaaaacgacg acagttgcac attcgagaaa
     5821 atgaaatctt agggatcttt caacaaggag agccgattca agtcaaagat gtccaacgga
     5881 tattgactaa tttggatcgt tttcttgccc agagtgagaa gaagcaaatc aaagccttgt
     5941 atcgtacctt tatagagaaa ataacctttg atcctaacca taaggagatg ttggaaatta
     6001 cgatgaaatt cacgcatgcc gtggtacaac aattgaatga gcaatatcaa atagccgtat
     6061 ccaaaacaaa ggatacggct atttttgtgt taaaaacacc ctttacgtta acgatctagt
     6121 ctaggttata tcaaacaacg ttagaggggc gctaacgctt ctctagcgat ttgaaatcgt
     6181 tagagaagta gttagggagg tccttatcat aaatagggat atttattaat gaatatttga
     6241 aatataattg tatataagaa ttaaaaagag tggggataga tatgaaaaag atgggtaagt
     6301 gtattttatg tgaaaaatac actgaactta ctaaagaaca tgtaccacct aaaaaatcag
     6361 ggaatactgg aggtaaaaag accagaactg gtaatttaga tgatttcctt aagagcgatt
     6421 ttacaaaagg tgattttccg aaggggatta aaaggaaacc tcaaggaaat gtttattata
     6481 ctggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
     6541 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
     6601 acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga
     6661 actaac
//
LOCUS       DACDKL010000132         6488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDKL010000132 DACDKL010000000
VERSION     DACDKL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.132"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1059
                     /locus_tag="IV329_002074"
     CDS             <1..1059
                     /locus_tag="IV329_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941242.1"
                     /translation="PYVFRNMFGHNISKLVSWKLTADCATVEENSEVYAAFQLRSKDM
                     SDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCYPNALIILSTWKDEDIKA
                     LKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQLGAKYVCKTRTDQRIYH
                     PNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMFFPYCLSDFLYFGDVDDM
                     INLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYISRMGGNEECSIRAYWEF
                     VKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDALHCYNFDFINWLNLYTG
                     KYEYKAEYEKYMDFVWEE"
     gene            1101..2435
                     /gene="gmhB"
                     /locus_tag="IV329_002075"
     CDS             1101..2435
                     /gene="gmhB"
                     /locus_tag="IV329_002075"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP8941243.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            2470..4107
                     /locus_tag="IV329_002076"
     CDS             2470..4107
                     /locus_tag="IV329_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP8941244.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            4209..4604
                     /locus_tag="IV329_002077"
     CDS             4209..4604
                     /locus_tag="IV329_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP8941245.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            4622..5353
                     /locus_tag="IV329_002078"
     CDS             4622..5353
                     /locus_tag="IV329_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941246.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            5377..>6488
                     /locus_tag="IV329_002079"
     CDS             5377..>6488
                     /locus_tag="IV329_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8941247.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSV"
BASE COUNT         2141 a          836 c         1357 g         2154 t
ORIGIN      
        1 ccatatgtgt ttaggaacat gtttggtcat aatataagta aactcgtatc ttggaaattg
       61 actgctgatt gtgcaacagt agaagaaaat tctgaagttt atgcagcgtt tcaattgcgc
      121 tcaaaggaca tgtctgatgt tccagtgatg ggtttagtta attgttgcaa agatgtagca
      181 attgtaatgc aggggccact atgtttaaag gatgatttct cttataatac tctggcctac
      241 tataagaaat gttatccaaa tgctttaatt attctttcga catggaagga cgaagatatt
      301 aaggcattga aaaggataga ggatcttgga gttatagtta tttgtagtga gaaacctgaa
      361 aagcctggac acttaaatat taattatcaa gttggaaaca ctttgcctgg aatagtcaga
      421 gcaaagcagc ttggtgcaaa gtatgtttgt aaaactagaa cagatcaaag aatataccat
      481 ccaaatgcaa tggcgttttt ctgcagttta cttgaacagt atcctgttaa taacgaagat
      541 gattggaaat taatacaaaa tcagcgatta ctgttactat caatgccgta tggagacatg
      601 tttttcccgt attgtttgtc agacttcctg tattttggtg atgttgatga tatgataaac
      661 ctcttctcga ttcctagtga tgtgagagaa aagggagctg tgtctagagg agtctcaaga
      721 agggagatat ctgaaaataa tttagctcca gaagttcagc ttcttcgaag ctatatttca
      781 cgaatggggg gcaatgagga gtgctctatt agggcatatt gggagtttgt aaaaaaccat
      841 gttattacta ttaacaaaaa tcaaattgat ttgtactggc ataaatatgt aggtcgttat
      901 tcaaataata ctatttatgg tacgtattat attgatgata gccaagatgc acttcactgc
      961 tataattttg attttataaa ttggttgaat ttgtacactg gaaaatacga atacaaagca
     1021 gaatatgaaa aatatatgga ctttgtttgg gaagagtaat taacttttat agtaaaaagt
     1081 actttaggga agggcatatt atgaaaacag taattatggc gggcggacga ggaagccgaa
     1141 tttcggaatt gtttccggat attcctaagc caatgattcc tatagatggc attccagttt
     1201 tagaacgaga attgtgttcg cttagagatc aaggctttaa tgatattatt ttgactgttt
     1261 cctatttgca tgaaaaaata gaggagcatt tcggagatgg ttcaaaatta ggaattaaaa
     1321 tcaaatattt tattgaaaaa actccacttg gcaatgcagg tgcattgtat tttttgttgg
     1381 atgaactggg tgatgaaccg tttttacttt tgaatgctga tgcagtattt gatgttgatt
     1441 tcaggcagat gattagattt catgttgaga ataacgcaga tgttactttg tttacgcatc
     1501 ctaactcaca tccgtatgat agtggattaa ttatatccga tgacaataag aaagtgcttc
     1561 aatggttagc gaaggaagat caaagacctg aatattataa aaaccgagta aatgcaggtc
     1621 ttcatgttat aaatccgaag gtgttgagag aaaatccatt aatggaaatt gtaaataaat
     1681 caatagttga aggtgggggg ctaccataca aaattgattt agatagaatg attctgaaac
     1741 cattggctaa cacagggcgt atgtattgct acgattctcc agagtatgtt aaagatatgg
     1801 gaacaccgga ccgatataga gctgtaatta aggactttca aaagggaatt attgttggta
     1861 aaaatctatc caatccacaa aaagcaatct ttttagatag agatggaacg attaataaat
     1921 acgttggttt tttgagaaat gcagaagaat ttgagttaat tgatggagta tcggaggcaa
     1981 ttaaaattat taattctcta ggttttctgt gtattgttat aacaaatcag cctgtaattg
     2041 caagaggtga agttactgtt gatgagctta atagtattca caataagctt gaaacaatgc
     2101 tagggcatgc tggtggattt ttaaacggta tttattactg tccgcaccat ccccataaag
     2161 gatatgcagg agaggttata gaattaaaaa ttgattgcaa ttgtcgcaag ccaaaacctg
     2221 gactgttatt agaagcatca aaggatttta acataaacct aagtcaatca tttatggttg
     2281 gagatagtga aagtgatgta attgctggaa aaaatgctgg ttgtaagaca gtactgatta
     2341 cagaagacat caccttgcct gctaattatg gccaagacta tagtgtctgt tcattgttag
     2401 aatttgttaa aaatcattta tgcactatgc aataattggt ttagtgaaac ttgtaaaagg
     2461 agtaattaga tgaaggtatt aattccagca gcaaaagttg ttgaaaaaga attgcagagt
     2521 ataggaatgt taccagcaat tatctatcca attaataaaa aaattgtctt tgattatttg
     2581 tataatcagt ataatgcaat ctgcgattca ttggatatta tctgttatga aaaagcggat
     2641 aaggttcata gaagactgaa aaactatcat gattccagaa ttcatatatg tgaccttcct
     2701 gaattagggg atctagggca aaccgtgtat tttgggttat cagatgaaga tgagcctgtt
     2761 ctaattaact tcgcagatac aattgttatg gagagtatgt ctgatataga aatggataca
     2821 ttctattatt ctgaagattt tgtgtcgagt acttggacat actttaaaat taatgaagga
     2881 ataattactg atatccacga taaagaggaa gcttacgtat ccgataaacg tgaaaaatta
     2941 tttattggcg tatttatgtt ttcttcgtca aaacttctga agagttgctt aagagaggca
     3001 gttgagagtc ggaaacaggg gggaataaac tcattttatc gggctttaca gctatacagc
     3061 gaacgttgtc cgctaaaggc cgtaagaaca gaaaagtggt ttgatattgg acacatcgat
     3121 aaatattaca atacaaagtt ggaagttaaa gcacgagagt ttaatcatat ctctatagat
     3181 aaagatcgag ggatattgaa gaaaactagc gatgacaagg ataaatttat tggagaaata
     3241 aagtggtatt taaaacttcc tgctgatatt gaatatgtaa gacctagaat ttttgattat
     3301 tctacatctt acgtagctcc ctatgttgat atggaatatt atgcatatca tacggttcat
     3361 gagttatact tatatggtga tttaaaatat aaccagtggc atgatctgtt tagccgtata
     3421 aagtttatct gtagcgattt taagcgatac accttaaaag atgaaaagat tagagagtca
     3481 ttggaagata tgtatttgcg aaaaaccatg cagagatttc aaaaattgtc tgaagataaa
     3541 agattttcag ctttttttga gaaacaaatt caaattaatg gtcgtaagtt tatttcttta
     3601 accggtgtaa ttgaacttct aaagaatctt atcccaagta tgctctatga tgtaaatgaa
     3661 ttttgtatta tacatggaga tttgtgtttt gctaatatga tggtagattc caattgttcc
     3721 tttgttaagg ttattgatcc taggggaaaa tttggaaaat atgatattta tggggacagc
     3781 agatatgaat tagcaaaact atttcatagt ttagatggta aatatgatta tatcatcaag
     3841 gatctattta ctgtagaata tgataccgag tgtgcagtga tagattatac aattaatacg
     3901 cgtgtaagag attacgattt atttgaattg tttaagtcgg tcttttcgga cgagattaag
     3961 gacgattttg ataagattgt attgatagaa gcgttgctat ttcttagtat gattccatta
     4021 cacggtgaaa gttttgatca ccaaatagtg atgcttgcta caggtatgga acttcttgca
     4081 agagtaagta ataaagttat actctaatat aaattgtgcc ttaatgaata acggttacaa
     4141 tactgtttta gcaatgtagg cattaaggca cgattatatt aattaataca atagaaggga
     4201 agagaaaaat gtatagtgaa tattcttttg tgtttgatat cgatggcact ttgtgtccga
     4261 taaagaagaa ggatgagcga tatgaggatt tggtgccgta tgagaatatg gttgaaaaac
     4321 tgagattcta taaggagaat ggtgctaaaa ttacattgtt cacctcacgc aatgttaatt
     4381 cctataaagg aaatttggga atgataaatg ctaatactgc aaaagtgctc ttggcgtggt
     4441 tggataaatg ggacattccg tatgatgaaa tcctgtatgg taagccttgg ccggggcata
     4501 aaggcttcta tgtagatgat cgaacggtta gacctgatga gtttttgaga tgctctgtag
     4561 atgaattaaa tgaaatctgc aacagttcaa aaaaaagatt ctaaataagg aggttagttc
     4621 tatggaaaaa aagatgacag ttatcattac gatggcaggt ttgggaagtc gttttcgaaa
     4681 aattggttat gcggttccta aatatatgat taatgccaaa ggaagaactt tatttgattg
     4741 ggccatggat agtttggttg gatacaataa aaatgttgat aagtatattt ttgttgttcg
     4801 tcaggaagac aacgcttcag cttttataaa ggagcattgt gcttactttg gtattgaaaa
     4861 cgtagaagtg attgaaatag actatatgac agatggtcaa gcaactacat gtatgttggc
     4921 tattccttat tgcaatcaag acaatgagat tatggtttac aatattgata catacgtgga
     4981 atcaaatgag atgaaatacg aagatatttg tggagatgga catattccat gctttcatgc
     5041 tgatggcgat cattggagtt ttgcaaagct taacgatcga aatgaagtaa tagaagtccg
     5101 cgaaaaagtt cgcatttctg ataattgtac tcttggtgcg tattatttct catcggcaaa
     5161 gctgtataaa gctttatatg aagagtatta tgcagactcc tctaatatgg agaagaatga
     5221 aaaatatata gcaccattat ataactttat gataaaaaaa gggttaaagg tgactattag
     5281 catagtagat gcgaaaaagg ttcatgtgct tggtactcca gaagagctca atgtgttttt
     5341 gaatcaagaa taaatagtgt ttaggagaaa atagcagtga ccagtaataa tctaattgta
     5401 aaaacattaa gcagatataa gaatactcca cttcctgtta aggctgcatt atggtttatg
     5461 gtatgtggtt ttttacagag aggggtttca atgattacaa ccccaatttt tactagagtt
     5521 ttgtctacag aacaatacgg tttatacagc acttatctat cgtgggaatc cttattgctc
     5581 atgattacat cgcttagttt gttcaaagcc atgatgaatc tttatgtaaa atatgataat
     5641 caagaacata ttctttcgac agtatccgga ttgactttag tactgactat tttttggttt
     5701 gttattgcta tttttattaa ggatatattg gcagaaattt taggattgac accagttcta
     5761 gttgtctgta tgttctcata ttttatattt caggcagaaa tacaatgttg gtctttacac
     5821 aaacgatata tatatgatta taagacttta ttagtagcta cccttgcagt tactgttggc
     5881 tcatctgtag ttggctgttt agcggttctg tttatatctc ctaccgctga ggcgagagcc
     5941 ttaatgaatg tcctagtgac tggtgttgtt ggcgtggctg tgtatatatc ggtatttaga
     6001 agatgtaagg attttaagga taatgaagtg tggaatttcg cagtagtatt ttgtgtatcg
     6061 ttgatgccac actatttatc tgaatttatt ctgaatggtt ctgataaaat tatgataaat
     6121 tacttatgtg gttcgtcaga tgttgctatt tacagcgtgg catattctat tggtgctcta
     6181 attaaccttg ttacaaattc tgtcaattcc acttttgcac cttatcagta tcaaaaaatc
     6241 aagtcaggag aatatgattt actatcaaaa agagctaatg aagttcttgg tttggtagcg
     6301 gctatgcttt ttgcattaat gctatttagt aaagaaattg tgttgatatt tggtgggcag
     6361 aaatatatcg acagtgttaa tttaattgta cctatatgta tcggtattta tttcaactat
     6421 atgtttcagc tgtttgctag agtacaggaa tactttgaaa agaaactcac tgtcgttatt
     6481 ccatcggt
//
LOCUS       DACDKL010000133         6451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDKL010000133 DACDKL010000000
VERSION     DACDKL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.133"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..1414)
                     /locus_tag="IV329_002080"
     CDS             complement(44..1414)
                     /locus_tag="IV329_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8941248.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            1652..2398
                     /locus_tag="IV329_002081"
     CDS             1652..2398
                     /locus_tag="IV329_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8941249.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            2395..3234
                     /locus_tag="IV329_002082"
     CDS             2395..3234
                     /locus_tag="IV329_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8941250.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            3235..4185
                     /locus_tag="IV329_002083"
     CDS             3235..4185
                     /locus_tag="IV329_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8941251.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            4326..5078
                     /locus_tag="IV329_002084"
     CDS             4326..5078
                     /locus_tag="IV329_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941252.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            5097..6248
                     /gene="queG"
                     /locus_tag="IV329_002085"
     CDS             5097..6248
                     /gene="queG"
                     /locus_tag="IV329_002085"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8941253.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         2208 a         1127 c         1313 g         1803 t
ORIGIN      
        1 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
       61 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
      121 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
      181 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
      241 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
      301 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
      361 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
      421 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
      481 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
      541 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
      601 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
      661 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
      721 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
      781 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
      841 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
      901 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
      961 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
     1021 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
     1081 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
     1141 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
     1201 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
     1261 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
     1321 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
     1381 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
     1441 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
     1501 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
     1561 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
     1621 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
     1681 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
     1741 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
     1801 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agccggtaaa
     1861 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
     1921 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
     1981 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
     2041 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
     2101 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
     2161 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
     2221 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
     2281 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
     2341 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
     2401 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
     2461 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
     2521 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
     2581 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
     2641 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
     2701 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
     2761 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
     2821 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
     2881 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
     2941 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
     3001 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
     3061 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
     3121 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
     3181 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
     3241 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
     3301 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
     3361 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
     3421 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
     3481 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
     3541 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
     3601 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
     3661 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
     3721 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
     3781 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
     3841 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
     3901 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
     3961 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
     4021 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
     4081 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
     4141 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
     4201 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
     4261 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
     4321 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
     4381 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
     4441 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
     4501 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
     4561 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
     4621 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
     4681 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
     4741 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
     4801 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
     4861 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
     4921 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
     4981 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
     5041 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
     5101 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
     5161 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
     5221 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
     5281 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
     5341 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
     5401 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
     5461 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
     5521 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
     5581 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
     5641 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
     5701 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
     5761 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
     5821 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
     5881 tggaaccaaa agtagacgaa gtatatccaa aattaaagcc aatgctttcg atgtctaata
     5941 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
     6001 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
     6061 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
     6121 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
     6181 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
     6241 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
     6301 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
     6361 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
     6421 aataaaaaaa catagaaaaa cgattacttt g
//
LOCUS       DACDKL010000134         6297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDKL010000134 DACDKL010000000
VERSION     DACDKL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.134"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..615)
                     /locus_tag="IV329_002086"
     CDS             complement(<1..615)
                     /locus_tag="IV329_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941254.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKK"
     gene            complement(715..1224)
                     /locus_tag="IV329_002087"
     CDS             complement(715..1224)
                     /locus_tag="IV329_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8941255.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1425..2294)
                     /locus_tag="IV329_002088"
     CDS             complement(1425..2294)
                     /locus_tag="IV329_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8941256.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2314..3435)
                     /locus_tag="IV329_002089"
     CDS             complement(2314..3435)
                     /locus_tag="IV329_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8941257.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3437..3832)
                     /locus_tag="IV329_002090"
     CDS             complement(3437..3832)
                     /locus_tag="IV329_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8941258.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3851..4648)
                     /locus_tag="IV329_002091"
     CDS             complement(3851..4648)
                     /locus_tag="IV329_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8941259.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4650..5438)
                     /locus_tag="IV329_002092"
     CDS             complement(4650..5438)
                     /locus_tag="IV329_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8941260.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5452..5928)
                     /locus_tag="IV329_002093"
     CDS             complement(5452..5928)
                     /locus_tag="IV329_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8941261.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6000..>6297)
                     /locus_tag="IV329_002094"
     CDS             complement(6000..>6297)
                     /locus_tag="IV329_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941262.1"
                     /translation="PTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVK
                     QNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1946 a         1365 c          891 g         2095 t
ORIGIN      
        1 ttttttatta attagttcat cgcccaataa tctctccagt ttcttagcat ctcctgactc
       61 aaccggaatt atccaagccc agtctaaata attttttaaa atctcatcat attttgcaaa
      121 tatttcattg ccatattccg tccttaaaat tatattttca gtgccagaca agtgatttat
      181 attgagttga taaaaaagat ctccatctaa aaaaatatca ttagaagaag atttccccac
      241 agtaatgacc attccatcag ctctatgtgt tacatacaaa taattgtatg acctcatttc
      301 acctaatgaa tcaattattt cttgcccttt agctctaata tattgatctc tattacctat
      361 ctcactctta tttaacaaaa gttttttcat gtcaaattta atcggctgtt ttatttttgt
      421 aaattcgctt ctactactaa tccaatccga gattaatttt tcaacatgtc ccagagtaaa
      481 ctctgattta ttcgctaaat actttttttg ttgtattaaa gacatctttc cccgaagctc
      541 aaaattgagc ctacatcgat tctctatctc ttcaaaaagc atcttatctt catcattctt
      601 atattcatca ttcataaaac caatccagct cctcgattaa gataatattt taatctcata
      661 caaaaactag ctctcatcaa aacaaccatt gttttgattt ctcatgaaat ttctttatac
      721 ttttctccga ttataaattc ctaacccctt gagaaaatta cgcatatatt tatctccgca
      781 aggcttaaaa tttcgttggc cttcttttct caaaaatgcc ccgatttcgc cttttgatgg
      841 atagatttct gcctcagcaa aaacgtctaa aatatcatca gtggtcatag acaaggcgat
      901 ttttaatttt tttagcaaca cattgttgat atatttgatt tccttgcttt tttgcggcgt
      961 gtctactatc ggcttatctt tttgcggacc acgcgccaag agaatcaggc catttaggaa
     1021 tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca ttattttctg cattatcttt
     1081 gttcttatcc tgtactttgg tcagtaccgc ctgagcttct tctttagtca gtggtacacc
     1141 gcctagctca aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat aacgcaatcg
     1201 caataaccta tcattgttat tcatttgttc ctccgaagta tttagtcttt actttcttac
     1261 gtactttttg tcttgctaag atttttagga tcaatgataa aaaccgcatc acttatgata
     1321 aggcgctggg tatcatgagt gaatgatatt gctgaataca aatagtccga ccacattaag
     1381 ttgatcggac ttcataagca ttttattgac taatactttt ttgcttaaga attgattcta
     1441 acatcttttg ctaaagttaa gcatcctata gttcctgcat tatctgctaa cttaggtgtg
     1501 acaatgtatt cttcgatatt tggcgtatcg acatagccgt taattagttc taaaaaacgt
     1561 ctttgtactt tctttttcag atgttcttgt tgcatgacac caccaccaaa aattataata
     1621 tctggtgaaa gcatcagggt cgtattacag gcacattgtg caatataatc cgcctcgatt
     1681 tcccagaacg gatcttctat agaaagatct tgtcccttgg ttcccgtacg catctcgata
     1741 gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt accagagaaa
     1801 gtatctcctg aatgtctctt aactaacgta tgccccattt ctggatggct aaacccttca
     1861 acaaaaatcc catcttgtat agctcctcca ccaattccag ttccaacagt gtaataaaca
     1921 acactggata aaccttttcc atgacctgaa acatactccc cgtaacaagc agcattcaca
     1981 tcggttgtcc aggcaattgg aatttcgaaa tgcttattca acgttccaac aaaatcaaaa
     2041 tttttccagg cagtttttgg agtagaggtg atatgtccat aggtctttga ttcacgatgg
     2101 atatcaatcg gtccaaatga ccctactcca atgctttcta attgttcttt atagtgatta
     2161 aagaaatcaa ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa actcactctt
     2221 tctaaaacct ttaaatcatc attgccaatg gcacaaacaa atttagtccc acctgcttct
     2281 attgagccat acatttctta ctccaccgtc cttttatcta ggaattcgtt cttccctgtc
     2341 taatagtttt tcgaaaaact catgtggttc cttttggtac caataggcaa cagaagaaat
     2401 atcgtcttga cgctcataaa gcttgatgtc atcatttcct agggtttgga aagtaacacg
     2461 taaattttct ttgaaactaa tggggtcggg taaatgccag cggtacaacc catgcatcgg
     2521 taaagcatca tcgccaaaac catgaactgg atttggatta ggttttccag cactaaaata
     2581 atcccgggtg tgatctcttt tagattgaaa aggatagccc ataaatagcg tattaaaatt
     2641 ttgagcatat gccctttctc caaagttctt tttatggaaa gcccaagcgc cgccaaaata
     2701 atcttctgct cctgtagatg ttattgtagg gaaatcttca tctccatcta tataaaactt
     2761 aaattctcct tccccccacc agtatctttc caatgcgcac aaagcaaagt aggttcctac
     2821 ataataccca gtgcctttga ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat
     2881 gggttcacgg ttccatttag catgaaaata catcgcatca tccggtactt cagtcaatgc
     2941 ataattaaat gtataaaaaa tgtgcttaat aaactcagga tgttcattcg ttaaagtaat
     3001 cttagctttt tttcgaaatg gcatctcaaa atagcaattc atccctgatg ttggattcac
     3061 tacgattggt aaggaattca cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc
     3121 taatggactt tctacagaag gagtggattc atcatcccaa taaatacgca aaattaaatc
     3181 acgtaagacg aaactcccag cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa
     3241 aattccaggg ccttccattt ctgctaaagt aattgtctct ccagcattta tcggaacaga
     3301 aggtgaccct tttctcccaa ttcctagatg actcgctgtc attgccgctt tccctttttc
     3361 accattttta ttttctggtg aaatagttct actttcctct cccttgtgga gccatatatt
     3421 atttaaacaa ttcattttat tcgtcctcta tcctttcttc acttttttga ttattcatat
     3481 agatgatttg ttgttgtgcc tccgcaataa ttgctccaat atctaaatta tcatttccta
     3541 actgcaaagc gattgacatt gccaagggaa gattcattcc tgcaattacg tgcactctgt
     3601 cagaaatata ggtaaaaaac ttttgattta cacttcctcc agccatgtca gttaaaacaa
     3661 ttatttcgtc tgtagtatcc actaactctt taatttgttc atctatatca tcaatttctt
     3721 cgacatatgc acataagcta ctaatttgat tatgcatacc tgttaaaaac tctagtgtct
     3781 ctttaaaacc ctcagctagt attgaatggc tagcaatgat caatcttctc ataattcaaa
     3841 tccttttcct ttacgctaat attccaaatg cagccataac catcgcaaaa acaataacga
     3901 gtagaatcaa aatggtcata ctgatttttt tcttactgag tagataatat gctgcagcag
     3961 taataattac tggaattaat gttggtaaaa ttttgtccaa cattggttgg attttcatac
     4021 taacttcacc aaaagtgaat ttaagtggcg ttgaaacact gataactgaa gcaattaatg
     4081 atccaactac tgttagcccc aatactgaag cagcttcagt taaataatta atcttttcac
     4141 cgtattcagt aacaatcttc attccctgct tataaccaaa ttcaaagcct ctcattcgga
     4201 aaaagaagat taccatgatt aaaatgaacc ataaaaagat tcctacagga ttattttcta
     4261 aagccatata agccgcaatt gatcccatga tggttgggat catcgtcatc aaaagagagt
     4321 ccccaatacc agcaattgga cccatcgtac caactttaaa gtcattaatc gcatcttttg
     4381 cttctaatcc ttgcttttcc tccattgcaa cacaagcacc taaaataatg gctgccagcc
     4441 aaggctgtgt attgtaatac tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat
     4501 ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt cacagatgca ccttgctgtg
     4561 tttgataatt aaaggtacaa actgacatca accaacgata gttcgctttc ctaagatcct
     4621 ttttcgttac ttgatatttt tcgctcattt tattcatcct ccccaataaa cccaacatca
     4681 gaagctcccg cagaagcagt gatttgtgca tctttttcag ctctatgata cacattaaat
     4741 gctaaagcga atcctatgat cgccgctcca ataataggta ccttcaaaaa agcggctaag
     4801 aaaaatccaa caagtaaata actaatgtat cgacctgtag gcataaagcg taacaacatc
     4861 gcaataccaa cagcaggcaa catttttccg gctaatgtta gcccatccat aaaccaatta
     4921 ggtaaaacat ccaaaacgta attaacagca gactgtccta aagtaacggc aataaatact
     4981 ggaattgctg cagataaacc aaagaaaatt ggccccaacc acaaaacact attcattttc
     5041 ttaaattctc gtttattcgc atattcctgt gatttgcgaa caacaaaacc atttaagatt
     5101 ttgacaatta catctagttg cacccctaac atacctacag gtagtcctat tgctaatccg
     5161 gcttctactc caagctggct accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc
     5221 ggtacagatg agccaccaaa accggcaact cctaatgaca tcaattgaac tgtaccgcca
     5281 actatcaaac ctgtttttat atcacccatc aaaattcctg tgaataatcc ccaaaaaact
     5341 gcgtaattta caaagatcat cggaatccca taataatcaa catgtttaat aaatgctaat
     5401 atagtcagca taataatttg aaaaatagta aaatgcatta tccttactcc tttatcctaa
     5461 ttttgattga atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa tttctacacc
     5521 agaatccaac aattttttat acgtgctgat attttcatct gatgcatagg ctctagaaga
     5581 tagctgtaat gcattatctt taacaacagt accgccaaca attagtcggt caattctaat
     5641 accgttatct actaaatgaa ggattgtttc cggctctttc acgatgacaa atactttttc
     5701 ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt tcttcagtga taattgatac
     5761 agccattcca gcaggtctag ctaaacgcat acttgatttt tggatgtcgt tatttgcgac
     5821 agaatcatcg atcaccatca ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat
     5881 aatcccatgc aataaacggt tatcaactct cgacataaca atactcataa atacttcctc
     5941 ctttatcttt cattttctta caagcaatta ccatgccaaa acaaaaagaa cttgataaat
     6001 caagctcttt ttacataatc ataaacgtat ttaacctcat gaatcgggat gtcgacacta
     6061 taataatgtt caacgacact gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc
     6121 tcactcgttt cattgaaata aatttcatct tccaaagtat tttgttttac taatcgttca
     6181 attaagcaac taatgtgaac atataatccg aaacacgtat tattatcaaa tgaaattcct
     6241 agatcatttt gtaagatgta tatagcatct gaaacccgtt ctaataattt agtcgga
//
LOCUS       DACDKL010000135         6295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDKL010000135 DACDKL010000000
VERSION     DACDKL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.135"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..915)
                     /locus_tag="IV329_002095"
     CDS             complement(64..915)
                     /locus_tag="IV329_002095"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8941263.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="IV329_002096"
     CDS             complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="IV329_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8941264.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3783..4589
                     /gene="proC"
                     /locus_tag="IV329_002097"
     CDS             3783..4589
                     /gene="proC"
                     /locus_tag="IV329_002097"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8941265.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4668..6224)
                     /gene="rny"
                     /locus_tag="IV329_002098"
     CDS             complement(4668..6224)
                     /gene="rny"
                     /locus_tag="IV329_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8941266.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         1846 a         1401 c         1096 g         1952 t
ORIGIN      
        1 aaaactgaag aataccattt taggattcct cagtttttca tcacctattt tacttcttat
       61 attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata
      121 tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact
      181 catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg
      241 atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata
      301 atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt
      361 atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac
      421 ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc
      481 cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag
      541 ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca
      601 gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt
      661 gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt
      721 ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat
      781 ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg
      841 atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt
      901 ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata
      961 gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca
     1021 gctttcacag aattttctct tcataatcca aacaagttct agtgattctt ataattctgt
     1081 gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag
     1141 ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca
     1201 atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc
     1261 ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac
     1321 attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat
     1381 aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa
     1441 caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt
     1501 ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc
     1561 cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc
     1621 ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa
     1681 acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat
     1741 ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct
     1801 ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg
     1861 attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt
     1921 gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa
     1981 ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa
     2041 taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa
     2101 atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa
     2161 ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa
     2221 gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc
     2281 atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga
     2341 taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg
     2401 tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt
     2461 gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac
     2521 tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc
     2581 agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact
     2641 tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg
     2701 attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac
     2761 ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa
     2821 ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt
     2881 cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc
     2941 tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa
     3001 ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct
     3061 ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat
     3121 ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa
     3181 cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac
     3241 catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc
     3301 accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt
     3361 tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa
     3421 caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct
     3481 ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac
     3541 ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg
     3601 ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact
     3661 tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga
     3721 cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata
     3781 acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat
     3841 taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg
     3901 cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat
     3961 cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag
     4021 aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa
     4081 tagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg
     4141 tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg
     4201 aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag
     4261 aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc
     4321 tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg
     4381 agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc
     4441 catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg
     4501 ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga
     4561 aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa
     4621 aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa
     4681 tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct
     4741 tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct
     4801 tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca
     4861 ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat
     4921 gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca
     4981 gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact
     5041 gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc
     5101 tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata
     5161 tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc
     5221 aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct
     5281 ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt
     5341 tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt
     5401 ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat
     5461 ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc
     5521 tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc
     5581 ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc
     5641 gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat
     5701 tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca
     5761 tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca
     5821 atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct
     5881 tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc
     5941 gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt actttctctc
     6001 agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct
     6061 tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat
     6121 tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga
     6181 ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata
     6241 ctatcttttt catttttatc atctatacag cttctatgat aaattattct aaact
//
LOCUS       DACDKL010000136         6257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDKL010000136 DACDKL010000000
VERSION     DACDKL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.136"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(315..398)
                     /locus_tag="IV329_002099"
     tRNA            complement(315..398)
                     /locus_tag="IV329_002099"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IV329_002100"
     CDS             926..1426
                     /locus_tag="IV329_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941267.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IV329_002101"
     CDS             complement(1616..1960)
                     /locus_tag="IV329_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8941268.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="IV329_002102"
     CDS             complement(2114..3034)
                     /locus_tag="IV329_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8941269.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="IV329_002103"
     CDS             3221..3712
                     /locus_tag="IV329_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8941270.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="IV329_002104"
     CDS             complement(3736..6111)
                     /locus_tag="IV329_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8941271.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
BASE COUNT         1909 a         1225 c         1031 g         2092 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaaca
//
LOCUS       DACDKL010000137         6205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDKL010000137 DACDKL010000000
VERSION     DACDKL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.137"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(90..515)
                     /locus_tag="IV329_002105"
     CDS             complement(90..515)
                     /locus_tag="IV329_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941272.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            889..1860
                     /locus_tag="IV329_002106"
     CDS             889..1860
                     /locus_tag="IV329_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8941273.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1892..3289
                     /locus_tag="IV329_002107"
     CDS             1892..3289
                     /locus_tag="IV329_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8941274.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3448..4071
                     /locus_tag="IV329_002108"
     CDS             3448..4071
                     /locus_tag="IV329_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941275.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            4820..>6205
                     /locus_tag="IV329_002109"
     CDS             4820..>6205
                     /locus_tag="IV329_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8941276.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFT"
BASE COUNT         2096 a         1052 c         1279 g         1778 t
ORIGIN      
        1 tcatccattt cgtctccttt acaatcatct tgcttcttta ctatagcaga aaagaagggg
       61 agaaacaaaa gaagatccaa cagacggcac tatagactgt agatgttgcc atcctcatcc
      121 ttcaaacggt aattaccgat ttttgtaaat atgactgttg tttctttttt attttctttt
      181 cctgaaataa tatattcgtc atctttggta gtgatcttga tatctgtcag cactgttttt
      241 tcttcagatg attcagaaga atcagttcct tgtgtctgat acgtgcgtgt ccattccatt
      301 tttttatctg taaatgtcaa ctgtcccgcg gataaatcca tatctttacg tacttttagc
      361 gtatatacac tgttggtggt atctgctttt tccgctttta agacagaaga atcggttgtt
      421 tcgtccgagg attggtcaga gccgccaaac aaatcacagc cagtcaatgt caaaagtccc
      481 gcagacaaaa taaatagtac gattaatttc ttcaaaatac ttcccccatt tgataggata
      541 aagaaattat accattggaa tgtaaaactt caaacggatg gaagagaaaa acgcacaaaa
      601 cacccacaaa tagagtaaat tttagaataa aattttaaaa aggcaaaaaa tccgccactt
      661 tcagaaagcg ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat taaaaatgaa
      721 ccggttcaaa tacttgttta agtataatta atagtgagag gaagttgctc tactgaagac
      781 tttagttata tggatattga tgacaagcaa cactatgcaa agaatgcggt catcataatc
      841 gaacaacaag aaagagaaat caaatgtaac tggcaggagt gagaatgtat ggcatcgatc
      901 cgtgatgtgg ctaagatggc tggtgtttca gtgggaacag tttcccgtta cttgaatggt
      961 cagcaactga aggaaaagaa catgaaaaaa atagcagaag cgatttcagc gttggactat
     1021 aaagaaaaca tcattgctaa agggttgaag aataatcgaa gcttctctat tggacttttg
     1081 ataaacggga tttccagccg ttttgggtcg gaagttgtct cagggatcga aagagtagct
     1141 gaagaaaatg ggtatagctt attattaagc gggtttgccg atcagccaga aaaaattgaa
     1201 caaaaaattg aatacctgat gaagcatgtg atcgatgggc tgattgtctt cttatccgaa
     1261 gaagagtggt ctggatttga aatgctcaca aaaatgagta ttccagttat cgcattgaat
     1321 tgtccagaag gaccagcagg tattgataca atattagtga atgatcgaga aagtgtagca
     1381 aaagtgatcg ctcatcatgg tgaacaagga cataaaaaaa tcggatttat tgctgccact
     1441 caaacagact acgtagcaag agaacgttta gcaggagcca aagaagcggt ccaaaatgat
     1501 ccatcgatcc aattagaggt ctttacagga gactattcta gaaaaagcgg ctattacggg
     1561 gcaaaagcat tattagaaaa aggggcaact gctatttttg tcagcaatta caatatgtcg
     1621 attggtgcca tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga tattgcgttc
     1681 agtcactatg attatttaga taaaaacgat ctgagtatcc ttccgaaaat cacgatcacg
     1741 ccaccaacag ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg aatcaaaaaa
     1801 gaagaggaag aaaatgaaaa agtgattgtg atggaaaata tcatacatgg aatcaactaa
     1861 aacgaactaa aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc cagcaaattt
     1921 ttggtggggt tctgcagcaa gtggcccaca aacagaagga aggatttcag gcgatggaaa
     1981 aggagagaac atttttgatt attggtacaa aaaagaaccg caaaagttct ttgatcaagt
     2041 aggacctgaa aaaacctcgc aagtttatac gaagtatcaa gaggatgtac agttgatgaa
     2101 acagactggg cataattcat ttcgaacatc tatccaatgg agccgtttga tacctgatga
     2161 atctggaaca atcaacgaag aagccgttcg tttctatcat cgttattttg atgagttgat
     2221 tgaaaacggt atcgaaccct ttgtgaatct gtatcatttc gatatgccga tgtatctgca
     2281 agaaaaaggc ggatggttga accgagaaac ggttgaagcc tatgaaaaat atgccaaaag
     2341 ttgttttgaa ttgtttggtt cgaaagtaaa aaaatggttt actcataatg aaccaatcgt
     2401 tccagtcgaa gcaggttatt tatatcgatg gcattatcca gaagagtcag atatgaaaaa
     2461 agcgatccaa gttggctatc atgaagcgtt ggcttcagcg ttagccatca aatcttacca
     2521 tgaaatggag caaggtggtg ttattggtat catcttgaat ctgacaccaa gctatccaag
     2581 agacgaatca aaccctgaag atgttaaagc agcgcaaata gcagatgcgt tcttcaatcg
     2641 ttcattcctt gatccagctg taaaaggcga gtttccaagt gaattgattg agatagtaaa
     2701 agaacttgat ctcatgcctg tgatccatgc agaggatttg acggttatca aagaaaatac
     2761 agtagatctt ttaggtatca attattacca gccacgccgg gtaaaagcaa aagaaacacc
     2821 aatcgatact aaaaacggtc caatgcctga ggattatttc gacaattatg aaatgcctgg
     2881 tcgtaaaatg aatccttatc gcggatggga gatctatgaa caaggaatct atgatatttt
     2941 gatcaatgta cgagatcatt atggaaatat cgactgcttt atttctgaaa acggtatggg
     3001 tgtagaaaac gaagcacgat ttatcaaaga agatggcatg atccacgatg attatcggat
     3061 cgaatttgtc caatctcatc tgaaatatgt ccatcaagcc attcaagaag gtgccaattg
     3121 tttaggctat catatgtgga catgtatgga taactggtcg tggaccaatg catataagaa
     3181 tcgatatggc tttatttcag tagacttagc acaagatgga aaaagaacaa tcaaaaaatc
     3241 gggtcattgg ttcaaagacg tatcagataa aaacggattt gaggcttaag aaagaatagt
     3301 tgagagcaaa tgcgtgaaat tctccattct taccattttg tgaaaaagaa gttcaattat
     3361 ttacgatctt tttcaacttt tttgtctttc tatttcattt tttgtatata atagaatagt
     3421 caaaattaaa taggagtgga ggaaagcatg aggaaaaaaa gaccgtttct tctcttttgg
     3481 ttattcggtt ttttcttact gcttatttta tccgcagcat tttcaggaaa aatgacagca
     3541 gatcttgctc agaaagaaac agaaaaaatc gtagcaagca aagaatcagt agataatttc
     3601 aaaaaagaat tctcaactta ttggtgggcg gagtataaaa taactgccaa ttacgaaaaa
     3661 ggcgaattgg acatttattt gccaaaagga aaagaagaaa atcaatttat taatatcgat
     3721 gacgtactta gtgcgatttc tctcagacaa tatcaattta aagacaagca tttgaccgtt
     3781 ttgcttttga ctcatgatgg cgacaaagta gcccgtttga aaaatggaaa agtgatcacc
     3841 gcttattctg aaaaagtaga caaaaaagtg gtcgccgatc gtttagcgga atggaaaaag
     3901 acaaatgaac aagtcaagaa aacaactgtc tcttctcaaa aaaacgagtc gacaggaaca
     3961 agtccttcag attctggcag tggacaaacg ataacgggaa gtgaagaaaa tcaagcctca
     4021 attattacga atgagtcatc acaaggacaa tatcaatcct atggacaatg aaaaacagag
     4081 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca
     4141 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tgtatcaaga
     4201 ggtcttgagc ctgccgttta actctgagcc acaaggagca atttcaaaaa atagctcctt
     4261 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttacca
     4321 agaggttgta aaattagcgt cctgacataa tctaataccg agggtcagct gattgaacac
     4381 cgtgtatcaa gaggtcttga gcctgccgtt taactctgag ccacaaggaa caatttcaaa
     4441 aaacagcttc tcatgtttta taaatattgt gacttgtggc cgaggagttg gcaggagaac
     4501 accgtttaag gaagaagttg tgaaaaagct gtcctgcatc aagaaataag gagctgcgac
     4561 aagaattttg tcacagcctc tttttttgta cgaataccga gaaactggct tttgaatacc
     4621 gtggagtgaa gcggtcacca caagactttt atctcttcat tagtttaatc gcttacatcg
     4681 cagcggtttt atgctgtgaa aagtggacgg aataatcttt agaaagaata aaaaaatctt
     4741 aagataacaa cttttttagg aaattttcat ttttcaggag caaaataaga aaaaatgtga
     4801 atagagagaa gtgatatacg tgacacaact taaaaaattg tgccaaaatc gtttagcaat
     4861 cgttttgaca gctgttttct ttttctggct gaagacgatc attgcctact atgcagattt
     4921 ttcattaggt gtggaaggta cgatccagta ctttatcttg tggatcaacc caattgcgac
     4981 taccttattg ttttttggcc tttctttgta tattaaaaag ccaaagccag cgttagctat
     5041 tcttttgatc attgatattt taaatacgct tttgttgtac ttgaatatta ttttttatcg
     5101 ggaattcaca gatttcatta cagtcaaatc tgtattaggc ttttcgaaag tgtcgcaagg
     5161 attgtcagga agctcctttt ctttgatgaa gccccatgat gtcatctact ggctggatat
     5221 cgctgtgttc attgggctac tggtttggtt gaaggtcaag aaaataccta tcaaaagcaa
     5281 ccctgtcggc aaaccgatgg ctatcgctgt tacctgtctt tcagggctta ttttttcagg
     5341 aaatctagcg ctgagcgaag ccaaccgtcc acaattgctg cagcgaacat ttgaccgttc
     5401 atatattgtc aaatatctag gaatcgatgc gtatactatt tatgacggaa tcaaaacagg
     5461 aatgactagc agtgttcgcg cgcatgcaag cagtaatggg attgatgaag ttttagatta
     5521 tacaaagaaa cattatgcag agccaaatcc cgaaacattt gggatcgcta aagggaaaaa
     5581 tgtgatcgtt ctccatttag aaagtttcca gcagtttttg attaatatga aggtagacgg
     5641 acaagaagtc acaccgtttt taaatagtat tttccagaat caagcaacga tcagctttga
     5701 caatttcttt catgaagtag gacagggaaa gacaagtgat gctgaaaata tgttggaaac
     5761 tggcacattt ggtttacctc aaggctcatt attcacagaa cttgggtcag acaatgtctt
     5821 tcaagccgca ccagcgatcc ttggacaaaa gcaaggctat acaagcgctg ttttccatgg
     5881 gaatgtagct agtttttgga atagagatca tgtatataaa aacttaggtt atgacaattt
     5941 ctttgatcga tcttattttg atgaatccga cgaaacgtta ggttatggga tattagataa
     6001 agatttattc agagaatctg cccaatatct tgagcatcta caacagccgt tttacacgaa
     6061 atttctttcc gtaaccaacc acacgcctta ttatacggat gacaagaatt tcgactttcc
     6121 atctttgaat actggcaata gcacagtaga taattatgtt cgaacggcac attatctgga
     6181 tcaatcattg gaacaatttt ttacg
//
LOCUS       DACDKL010000138         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDKL010000138 DACDKL010000000
VERSION     DACDKL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.138"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV329_002110"
     CDS             <1..395
                     /locus_tag="IV329_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941277.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IV329_002111"
     CDS             398..1228
                     /locus_tag="IV329_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8941278.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IV329_002112"
     CDS             1244..2095
                     /locus_tag="IV329_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8941279.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IV329_002113"
     CDS             2092..2451
                     /locus_tag="IV329_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8941280.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IV329_002114"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IV329_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IV329_002115"
     CDS             2624..2929
                     /locus_tag="IV329_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941281.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IV329_002116"
     CDS             2929..3240
                     /locus_tag="IV329_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941282.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IV329_002117"
     CDS             3407..3823
                     /locus_tag="IV329_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8941283.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IV329_002118"
     CDS             3823..4491
                     /locus_tag="IV329_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8941284.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IV329_002119"
     CDS             4488..4865
                     /locus_tag="IV329_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941285.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IV329_002120"
     CDS             4862..5161
                     /locus_tag="IV329_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941286.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IV329_002121"
     CDS             5403..5819
                     /locus_tag="IV329_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8941287.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDKL010000139         6001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDKL010000139 DACDKL010000000
VERSION     DACDKL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.139"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..185
                     /locus_tag="IV329_002122"
     CDS             <1..185
                     /locus_tag="IV329_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941288.1"
                     /translation="DKNQGFLQFYGAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVS
                     ATFSIRFAKKIAEPKQ"
     gene            258..737
                     /locus_tag="IV329_002123"
     CDS             258..737
                     /locus_tag="IV329_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8941289.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            959..1102
                     /locus_tag="IV329_002124"
     CDS             959..1102
                     /locus_tag="IV329_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8941290.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1680..3263
                     /locus_tag="IV329_002125"
     CDS             1680..3263
                     /locus_tag="IV329_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8941291.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3700..4884)
                     /locus_tag="IV329_002126"
     CDS             complement(3700..4884)
                     /locus_tag="IV329_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8941292.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4909..5916)
                     /locus_tag="IV329_002127"
     CDS             complement(4909..5916)
                     /locus_tag="IV329_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8941293.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
BASE COUNT         1860 a         1244 c         1131 g         1766 t
ORIGIN      
        1 atgataaaaa tcaaggattc ctacaatttt acggtgctgc atatgctgtt ttaggcgtaa
       61 tgggtatttt aacagctttt ttcaatcaac gctttattgc cttggtcttt ttactgatcg
      121 taattattgt ttctgccacc ttcagtatcc gatttgcaaa aaaaattgct gaaccaaagc
      181 agtaaataat tctctttttc cctaaagtgt tctacaataa aagagtacaa tacgattgac
      241 ttgaggagtg aatgcttatg ttacaacaat acaaaaaaat catggttgcc gtagatggtt
      301 ctgatgaagc tgaattagct tttaaaaagg cggtcaatgt agctatccga aataacggag
      361 aacttctatt agcacatgtc attgacacac gttctttcca aaccgtttca tcctttgatg
      421 gaatgcttgc agaacaagct actgaaatgg caaagcaaac attggcagat tatgaaagca
      481 atgcgaagaa agctggcttg aataatgtaa cctctgtcgt agaatatggc tcaccaaaac
      541 aaattattgc cagagaaatc ccagaagata atcaagtaga tttgattatg ttaggagcca
      601 caggattgaa tgctgtagaa cgtttattta ttggttctgt ttctgaatat gttattagaa
      661 acgcagcttg cgatgtgctt gttgtccgaa ctgatttaga aaaccaattg ccagctaaag
      721 accacaccaa agcttaacac gtagacgacc tgccaactat cggcaggttt ttttgcacaa
      781 aaaaccgtga taaaaacaaa agacgcggca agcactcatc ggtctttata atgtttaaaa
      841 taaagggaaa acagtcaatg agttcaagta aaataaaaaa agtgaacacc agggcgatta
      901 gtcgccctgg tgtggtttaa aacgacctaa gcatgccaaa gcttattggt tcgtcaatgt
      961 ggaagtttcc gatgaatctg atacgaaaaa atatgaagta tcgatggcgg ataccgattt
     1021 tatcgccaaa ctaaaatttt atttcgggtt cgggtaccgc gagaaggcaa gtgctacatt
     1081 cggtaaatca ttcaagaatt gatgaaaggc taccgcttgc cattgcaacc acaacgatac
     1141 tcctgaacgc gatgttggtg agatttccaa agagaagagc tgtttaatgc aacggtatta
     1201 agcaaatggc aaaaggaagt catgtagaca aagtttttta tcaaacacca tatgatttcg
     1261 cctgaacgct ggtttagctc agaatacaat gatgtggtcc acgagacatt accctcagtc
     1321 agataaaaaa agctctctga cagaattaca catcgataaa tattaagtaa atgtcagcgc
     1381 tagttgaact ttctgacact ttcaggtaca ctggaaaaga caactacacg aaggagggaa
     1441 aatactatga tgcgcatgac taaaataaca agtaatgaaa attaccaacg ataccatatc
     1501 gcatttagcc atgcaattgg ttttacagtt gctgttgacg ggacaagttt gtccaaaaca
     1561 gttgaattca cgtaaaatca atggccatct caaggtattt ttctcatgat aatctgagtt
     1621 taaataacac aggccaggga taaccatggc ctttttgagt ggtctcaagg agagagatca
     1681 tgatcataca agcaatttct attaaaaaaa caatcggcgg gaatccatta tttgaggatt
     1741 tatcctgtca aattgaagct ggtgaaaaaa ttgccttagt cgatatcaac ggaactggca
     1801 aatcaacctt tttacaaatc ttgaccggca gagaaggtgt cgacagcggt gttatcagtc
     1861 gcaaaaaagg cttaaagatc gggacggtcg aacaagaact tacagtttcc gaagcgaccg
     1921 tcaaccacta cttgctccat tctgccgctg agatacagga cctcaaacag caaatgagtc
     1981 gctacgaagc attgatgaca gaaccagata caaacttaga gaagtgtctg gcccgttacg
     2041 gtgagctgca gcatcgcttt gaagaactag gcggttacgt attagaagac cggattgcag
     2101 cgatcttgca aggcttaggg attccacact gtcaggaagc acggttaaca gaacttagcg
     2161 gaggcgaacg agttaaagtc gctttagcca aaattttagc agccgatgcc gatttacttt
     2221 tactcgatga accgaccaat catttagatc ttaacagtat tcgctggttg gaaaactatt
     2281 tacagaatac aaaaaaatcg gttctggtcg tttctcatga tcggcaattt ctcgatcaag
     2341 tcaccacaaa aacatgggaa ctggaagaag gtgcattgat tgagtatccc ggaaactaca
     2401 gccgttttcg agtcttgaaa gaagctcgct tggctgagct gacaaaaaat tacgaactgc
     2461 agcaaaaaga agtccagcgg ttgaaagtca tgattcgtcg ttttcgtcag tgggcacatg
     2521 aaggagacaa tgagtccttc ttcaaaaaag ccaaagaact ggaacgccgc ctggcgaaac
     2581 tgacactcgt caaaccgcca cctcccccga aaaatcggct gcaaagcttg tctaatggtg
     2641 gaaaatctgg caaagaagtt ttcatcattc aaaacttgca ccagcagtac gctgatcaag
     2701 tactattcaa agacagcagt tttgcagtct atcgtggaga tcatttagcc atcatcggcg
     2761 acaatggtgc aggaaaatca accttactca aattattact cggtgaggaa aagccttacg
     2821 gtggcaccat caaattaggt agtagcttgc aaatcggtta tttgccgcag cagctgcagt
     2881 ttgccgatcc cgatagccgt ttgctggctt acgccatcac attgactgga aacgaagaag
     2941 tcgctcgacg gcaattagcc aaaagtggct tttatcaaag cgatgtgggt aaacggatca
     3001 aagatttatc cggtggtgaa aaaatccgtt tggcgttgct gaaactcttt ttagaaaaaa
     3061 tcaacgtcct gattttggat gaaccaacca atcacctcga cagctatgct cgagaagaaa
     3121 ttgaagaaat gctgcaagac tatcaaggaa cgctcctagc agtcagccat gaccgctact
     3181 ttttgcagca acacttccaa gaagcactcg ttattgatca ggagcagatc agccgccagc
     3241 cgctcggcca actgattgac taggacgttt tttcgcaaca aaaaacttga ttttcatacc
     3301 tgtgctttca cgattcaggc aaagtacagg tggaaaatca agtttttatt attcgatcaa
     3361 tgaatgtttc ggatcggttt tctagctaaa cataaagcga agaataaagt cgacagccca
     3421 cgaggctgat ctaaaaatgt tttttctttt tacatatgta atgtccctta taaagatgaa
     3481 tgaaaataaa atattgggat tttcctgaaa atcaataaat aaaatgccag tcttttccct
     3541 tttgaccact tttccaagca aaattcccta agaagatcct gtaaaaaaga agggaaattg
     3601 aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa cacatgcaag aattccctct
     3661 tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca gacgctccac
     3721 atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta cttttgattc
     3781 ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat cgatttcaca
     3841 accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat tctcgccgat
     3901 accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac cgccgatgta
     3961 ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt tttcatagtt
     4021 cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac cagatttctt
     4081 gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta aatatgggag
     4141 aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta atggtgtgaa
     4201 tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata caccatttcc
     4261 taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt ttgccgcacg
     4321 ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt tgaaatcttc
     4381 ataataagac accggtaagc tatataaata attttgcttt ggcattgttg agtggaaaga
     4441 tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg ctttgatccc
     4501 cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa tgcggtccat
     4561 tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa caacacggtg
     4621 ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca actgatctaa
     4681 cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga atttttgatt
     4741 atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga cgatcccttt
     4801 cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg aactcccagc
     4861 attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt acagagcaga
     4921 tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt cttttaatga
     4981 agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt ttttctgtaa
     5041 gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca attgaatcat
     5101 tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt gatccgtttc
     5161 taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat atttcattga
     5221 ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg ttaaaaactc
     5281 ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg caaaatcgac
     5341 aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat ttgcttgtgt
     5401 caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca caccaattcc
     5461 ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca agttgcccat
     5521 tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata gttgttcaat
     5581 aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg cctgcaaatg
     5641 ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt cttctggctc
     5701 gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg tctcttttgt
     5761 cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg cattttcgac
     5821 ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag cttccaggtt
     5881 ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt ctagatatat
     5941 tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt caatcgtttt
     6001 c
//
LOCUS       DACDKL010000140         5961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDKL010000140 DACDKL010000000
VERSION     DACDKL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.142"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..947
                     /locus_tag="IV329_002128"
     CDS             54..947
                     /locus_tag="IV329_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8941294.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            1081..1206
                     /locus_tag="IV329_002129"
     CDS             1081..1206
                     /locus_tag="IV329_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941295.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1340..1972)
                     /locus_tag="IV329_002130"
     CDS             complement(1340..1972)
                     /locus_tag="IV329_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941296.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2120..2662)
                     /locus_tag="IV329_002131"
     CDS             complement(2120..2662)
                     /locus_tag="IV329_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8941297.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2797..3186
                     /locus_tag="IV329_002132"
     CDS             2797..3186
                     /locus_tag="IV329_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941298.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3190..3954
                     /locus_tag="IV329_002133"
     CDS             3190..3954
                     /locus_tag="IV329_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8941299.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            4083..5030
                     /locus_tag="IV329_002134"
     CDS             4083..5030
                     /locus_tag="IV329_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8941300.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            5160..5900
                     /locus_tag="IV329_002135"
     CDS             5160..5900
                     /locus_tag="IV329_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941301.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         2092 a          967 c         1176 g         1726 t
ORIGIN      
        1 ttttacgtaa aagcctttta agtaaaataa aacggataga aaagagaaca tatatgaact
       61 attattcaaa aaataaggaa aacttttatc aaaaactgac cgatgatccg cttttttctt
      121 cattgaccga ttatctatat gaacatcgag aacaagaaac gattctaaga gaattgaaaa
      181 aagaattttc gcaaaataaa ttttcccatt ttcttgattt attgatcgat gcaggattaa
      241 tcaagaggga agaacgaaga tatcatttga actttccaat atttgatcct aaagattact
      301 tgcaacaagc cacttcggct gctgaaacca tagctgaaca attgaagaga ctctctgttg
      361 atgaacaaaa attagcgatg ggagaagtta tttgggcgta ttgtttcgaa gatgaacgga
      421 aagaagcgta tttttatgga gtaagaaaca gccgagagac agaattgctg aggacaactg
      481 caggaaatga gaagtaccgt tttatcacac tttcctctat agaacacttc ccgcttactt
      541 tggctaatta cttttttgta cagaagaatc agcttcctgt aacgaaggca ttcaaagaat
      601 tagccgaact tattggagac gtaaatgaat cgtacttttt tgatcaaata gaagttatcg
      661 ttgacaggat acgtaagaat aaatataaaa atcgccggcc tagtattttc catcagtctc
      721 tacttgtgac gaatactata aaggaagaag aatcattcac attagaatta cctattgtcg
      781 aaaaaaacaa ttttgagatt gaattaccaa cattggatcc ttcattgaca atggaggaaa
      841 tggcctttct taaacgacaa atcttttctg agctgagcaa gaaatttata ccgcatgcat
      901 tttcttatat caaagaatat ggaacagttt tagtgagtaa aacctaagag agcagtaagc
      961 aaaaattata ctgactcttg caggaggagc ctttctcttc ctttatggtt aaaagaatta
     1021 tttgagtatt ttgcctatct accacctatt agattcacta catggtctgg aaacaaagga
     1081 atgcaaaaaa atggtgagaa atgtggaatg acaaaggaag tagtgataag aaaagttaga
     1141 tttttaaata atcaatatta tgattcagtc aagtatggca ttttatggga agaactagct
     1201 gattagatta ggaagtccat ttgataatcc agaatcacta agaaacgaat ggcgagtcat
     1261 taataattta tccaatcttg ggttattctt ttagattgaa gtgtttaaaa ctaaaaaagc
     1321 atttatgaag tcgtaatagt caggcttcaa aatgcttttt attttgtttg ttttttcttt
     1381 gccacataaa tacacctaga ctgacgatga agagcatatc caacagattt cctgcgactt
     1441 ggatcgtcga agggttggtc agtggcatta aagcaaggac aaaaaaggaa aacaagctgt
     1501 aaataaaaca ggtgacagga actaatcgaa agaagagcgg acatttcaat tggctaaaaa
     1561 tgagtgaaaa aataaaataa gtaaaaatgg ataaaatcac gactaaacca ttcatgataa
     1621 agaagaaaag cgtattgtga ttaaacgaat aaaaaagaat tgctaaaaaa gaaaacgttt
     1681 taaatgcaat caatacgctt gaagcttcgt ttaacgtacc aggatgtcga tgaaaaaaat
     1741 aggcaccgaa ataaacagaa aacagtaagc cgcaaagaga agacaaaatc aaaaaaaacg
     1801 gattttcttc ggcttctgtg atatcagaat gatccaaaag caaccagcct aatgcactag
     1861 aaggcgcatg tgaggtctta aggtatgaat aacatagaaa aaataagatc gtcaaagttg
     1921 caaaaaaaga gcctaaatgt aaaaaattag tggttctacg ttgaatttcc ataatatctc
     1981 caaattaaat tgataagtat ataaagtata ataacaaatt gatgagtata tgtcgatgtt
     2041 ttttaatcaa aataataaat aaaaactgtc taacatttaa aatttaattt agatattaga
     2101 cagtttttaa gtttgtgagt tattttcctt gtcgttttct cactttttta gtccagaaac
     2161 cacctgaaat atattttggc tcataagaaa tgataaaagc acgctcttcg atttctttga
     2221 tcaggcgata cagttcacgt tcgctttttc taggagaaag tatttcgaga actagccgct
     2281 caccttccag ccccattgca gaagtctgtg tcacaccata accgtgttct ctcagtatac
     2341 ccggcaaact tttatcttct gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc
     2401 ctaatgccaa tttgtcttca atcaaaattc cgacactgat acctactgcg taacctaacg
     2461 catagacaaa taaattcagt ggattatcta aacgatctaa aacaagggat aagccgacga
     2521 tataaatcgt gatctcgacc atactgacaa gtggagcaat cagtcgatag cctttcattg
     2581 tcaacatgaa acgtaacgtg ttcagagtga tataagcaaa attaatgagg aagatcatcc
     2641 ccaacatttt tggatcaatc atttgtaaaa ctcctttcat ttatataaca acaatatcaa
     2701 aaaaaatcgt aagtgaagaa aaattgaaac tttatacata caaaaaaatg ataaaatgat
     2761 tataatagaa ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt
     2821 tgtgccagcc acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa
     2881 cagccaattg aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta
     2941 agcgtttttt tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt
     3001 ttgaattttt ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct
     3061 ctgtgcttat ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg
     3121 ttccagacaa ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt
     3181 gagtaacaat tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa
     3241 gttgttctga aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg
     3301 cagacaagta aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt
     3361 gtgatcctgc cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca
     3421 tcctatacat tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag
     3481 catttagaaa aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg
     3541 ggaatgacaa catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg
     3601 atgggttccc ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat
     3661 aattatgtcg taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg
     3721 tcattgcagc cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac
     3781 gaaaaaatcc cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac
     3841 ggaaaagaaa ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aataccacta
     3901 atggaagaag cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa
     3961 cggaaaatca ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact
     4021 ctgttaggaa aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga
     4081 gcatgcccac tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc
     4141 gagtcatcaa tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa
     4201 tggctgaact ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc
     4261 agatcatcaa attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt
     4321 tgctgatggg aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa
     4381 atgatgcctt cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag
     4441 acttcgattg gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag
     4501 gatatgatta tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct
     4561 tagaaagaga ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa
     4621 tttcaaggga aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct
     4681 tgcggtttcc aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt
     4741 ttacagataa aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag
     4801 ggattataaa tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg
     4861 tatttttaga ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta
     4921 tgggtgaagt atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag
     4981 gttatcgtcg tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac
     5041 tatatatata tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg
     5101 aaaacaatga atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa
     5161 tgaaagaaat cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa
     5221 aattaaccaa cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac
     5281 agaatcagtc cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga
     5341 aaatccgtcg caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt
     5401 tggaaaagca taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat
     5461 cgacaggacg aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa
     5521 ggacagagtc caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt
     5581 cttattttgt tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa
     5641 tttctgagga tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg
     5701 atacagaacc gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca
     5761 atcgaacaaa aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc
     5821 atggtatctt agaacgctca ttacaaggat tgatcgatga tcaaggaaac tctttgggag
     5881 aaaatagcta ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa
     5941 acgctgcggc gtttccttat t
//
LOCUS       DACDKL010000141         5941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDKL010000141 DACDKL010000000
VERSION     DACDKL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.143"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(153..638)
                     /locus_tag="IV329_002136"
     CDS             complement(153..638)
                     /locus_tag="IV329_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941302.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(777..992)
                     /locus_tag="IV329_002137"
     CDS             complement(777..992)
                     /locus_tag="IV329_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8941303.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1216..2016
                     /locus_tag="IV329_002138"
     CDS             1216..2016
                     /locus_tag="IV329_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8941304.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2035..3903
                     /locus_tag="IV329_002139"
     CDS             2035..3903
                     /locus_tag="IV329_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8941305.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3896..4387
                     /locus_tag="IV329_002140"
     CDS             3896..4387
                     /locus_tag="IV329_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8941306.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4375..4929
                     /gene="srlA"
                     /locus_tag="IV329_002141"
     CDS             4375..4929
                     /gene="srlA"
                     /locus_tag="IV329_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8941307.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4947..>5941
                     /locus_tag="IV329_002142"
     CDS             4947..>5941
                     /locus_tag="IV329_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8941308.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2079 a          947 c         1199 g         1716 t
ORIGIN      
        1 caagtagaga agtgagtata ttcataagag aaatagctgt aacgaattat taggttaatg
       61 caaatgctta tttatactcc gtataacttt ctcagaaaaa atgataccaa taatgacttg
      121 ctagtactgt aaatccgtat aatatcatct aatcaaaatg gtgttccatt tgcatctgtc
      181 atcgtaatcg atccttcggg tgacactcga taaaaaccaa cgcttccaga tcctccttgg
      241 gaacggattt gtttgctgga agctgtgaac aaataatctc gatcaatttt ttgataaaag
      301 ctaataacaa tatcgtcact ttgtaattcg gaacggtggg caataagaaa tagaagtgct
      361 ttgtcagagt tttctaatgg aagagaaggt acagtgcttt cttgcatttc agatgttgaa
      421 gaaatgctaa acgactgaat acttgattgg ctatgttgcg aatctacatt accagtagaa
      481 gtattagtaa tggaagtagt agaagtactt ttagttgaac caacctcagt tgttgaaatg
      541 gctgctgaag attctttaac agaattcttt gttttatatc cacaggcaga aaaaagtatt
      601 acagttccca acaagcctgc gtaaatccct ttatgcatga tagctcctcc taaaatagat
      661 tatctttatt cagtatatca cttaaacaaa gaaaagcaga tggtttaaaa tgaaaaaacc
      721 ttcccaataa agggaaggtt catgaagata ataacttttt taataattat agctttctaa
      781 tatctttcat caacttttct agtaatcatt gggaaaaatt tcaagcagta gatcgcacaa
      841 atacctacga cgatataaat gatcttcgca aaaatagttg ttgaaccacc agcaatcata
      901 gctactaaat caagttcgaa caagcctacc aacagccagt tcaaccctcc cacaatcaat
      961 aaagttaatg caatagcatc taaagttttc attagaattc tcctttctta aatgataaaa
     1021 caattatata tcaaaaagag aaacattgct tatataacgc tcaggaattt tagcaatcta
     1081 tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac acatttttta gctgaagctg
     1141 taaatttcat gtcttaattt tttttagtga gcttttatac tggatttgta agaaaaaaga
     1201 aggagaggat cacatatgtc ggattggttg gatattgcag gaaaagtagt aattgtaact
     1261 ggaggttcct caggaattgg aagaagtatc gtcgaaaatt tgttgaagca gaatgcacaa
     1321 gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg aaaacctact atctttaaaa
     1381 gtcgatgtgt cttctaaaac ggacataaaa gaagggatat ataaagtaat gaaacatttt
     1441 aagacgatcg atggtctagt caataatgca ggaataaata ttccttctct tctaatagat
     1501 agaaatcatc caaaaagcaa gtatgaatta tcagaacaag tttttgataa gatgatcgct
     1561 gtcaatcaaa aaagtgtata tttgatgagc caagcagttg gaagaatctt ggtgcaaaag
     1621 ggatcaggag tgatcgtgaa tctttcttct gaatcaggat tggaaggatc agaaggacag
     1681 agctgttatg ctgctacaaa agcagcgatg aacagtttta ctcgttcttg ggcaaaagag
     1741 ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag ggatactgga agaaaccggt
     1801 ttgagaacac aagaatatga agaagctctt tcttatacga gaggtataag cgtcgaacag
     1861 ctgagaaacg gttacagtcg aacaagtacg atccctctag gaaggagtgg taagctgcaa
     1921 gaagtggcgg atctagtctg ctattatctt tcagaaagat caagttatat cactggagtc
     1981 acgactaata tttcaggagg taaaacaaga ggataaagga taggggggac gcacgtgacg
     2041 ctagtaaaca gatggtacca attattgaaa ttactagtca ctcacaagaa agtttcaatc
     2101 caggaagtgc aaaaagaaat caaaaccagt aatcaaacca taaaaaaaga tattggggag
     2161 ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa aaaacaaaca ttttgaactt
     2221 gaaatttatg attttgattt ttttgatgag atcatgcaag gaaaattgaa aacagccagt
     2281 gatttcaatt cggcatccaa aagaatcgct tatttactaa ataggctgat cgaaacagag
     2341 gatttcttac gtatggatga tttgtctgaa gaagtcggtg tcagcagagg aacagtggtc
     2401 aaggatctaa agacattaaa agagatgatc aatgattttg atgtaaagat tacaggcaca
     2461 ccaaataaag gactaagaat aataggagat gagctagaat tacgactact tttattttat
     2521 tttgtttctc cctacttttc agagacgaaa acagaacagt ttctagaaga aaaacaagca
     2581 atcaaacatt ttcagaagaa gacgaatgct agtaaacaag aaatcaccct tttaacgaaa
     2641 gttatcggta tttctttgaa tagaatctct agtaactatc tgttgaagaa gcccatcaat
     2701 cgttacagtg ggtgttttga atatatccaa gaattcaatg aactgatcaa tcagttggaa
     2761 gaaatctatg aactaacttt gagccaatat gaaaaagatt ttatcagttt tcctttgaac
     2821 atcatcaaca caagtataac gatgataagg aaagctaact ctgatttgcg cttatatttc
     2881 gaccccatga tgatgcgtat tcgacgatta gatctcattg atctggatga gtccttctta
     2941 tatcaagaga tgaaacagca cctctcttta ttgataaatc gggtaatttt tcgttatcga
     3001 ttgactgatt tattttatgg tgagatagaa aaaaagtatc ctttttctta ccaaatcgct
     3061 agcgaatgca tacaagcatt agaagctatc ttgcaacata agatctctaa agtagagatc
     3121 agctacctaa cgttgtattt ggagttagca tttcgatatc aaaaagagca tttttcctct
     3181 aaaaaagtag ccattgttca tacgacagga aaaggaacag cattaatgat ccaaagacaa
     3241 atcaaaagga tattggggag ggatatacaa ataacacatg tttcagaaga acaatatgaa
     3301 aaagtagatc taaacaaata tttcgctgtt tttacaacga ttccgttaaa aaatgtaaat
     3361 gaagagacac cggtgatcca tttgactaat ctttttgatg actcttggtt aagggcacaa
     3421 tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt ctactgtttt tttctatcaa
     3481 atatggaaag aaaaaaagac gtatcaagaa tatttaaaca aattagtcgc tcctttgatc
     3541 agaggctatt atgtggatga agaatttgaa aaaaggatct tgaaaagaga atatcagcaa
     3601 agcacatttg ttaacaaaga atttgctttt cctcacacaa ccaaccagct atctcaacac
     3661 attgttttat cagtagggct aataactgat caaggtttag cagaaacagg aggaaatgga
     3721 gttatttttt tatttgccat acctgaagaa atgacggctg aagttgagaa tgaattattt
     3781 gagctatacg atatcctatt tgaactcata aaaatgggaa tagatgagtt tattgaatta
     3841 gcaccgacac aagaatcatt tttaaaacta atcaaaagta aaggagagag acttcatgaa
     3901 tgatatgtat ctattcatag ttttcgcagc ttcagcttat gtgctgcaag cagttatcag
     3961 ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa ctgcgaagaa aaggaaaagt
     4021 agcgattggt aaacgacctg ggaaaatccg ttcaggcacg attgttatgc tagctataga
     4081 cgatcatgat caagttttag aagtcagaaa aatgcaaggg gttacatctt tggcaaaatt
     4141 tcattcatta cctgtctatg cgggtgaaga tatccattac atcgatcgtt accatccctc
     4201 agttcgagcg gaagatcgtt tgacacaagc agctttagaa aatgccagag aagtatactt
     4261 acgaatagag gcaggaaatg aaaaaagtga acaaccttca ttgcctttta ttgctatgct
     4321 caataatttt cggatatgga aaagtacgat caaagaaaaa aggagtgaca aacgatggat
     4381 atcataacaa aatttgctga gggatttatg aggctgttcc aaacaggtgc agaaacattc
     4441 ataagctgga tgacaggaat cgtaccagtt gtcttgatgc tgatggttgc aatgaatacg
     4501 ctgattgctt ttttaggtga agaccgagta acgaaagtag ccaaattatc agcgaaaaat
     4561 ccgcttactc gttaccttgt gttaccgttc ttatctgctt ttatgttggg aaatcctatg
     4621 tcatttacaa tggctcgttt ccttccagaa tactacaaac caagctatta tgctgctcaa
     4681 gctcaatttt gtcatacgag caatggtgta ttcccccata ttaatcctgg agaattgttt
     4741 gtatggatgg ggattgctca agggatcgaa accttagggc taaattcgat ggagctggct
     4801 atccgatact tgcttgtggg actgctgatg aactttattg gtggttggat aactgatttt
     4861 actactggat ttgtatgcag acagcaagga atcgttctta gcaaaaaagt agagctttca
     4921 gttgattaga gaaaggagag ttttaaatgt atggtgatat taagatcgta agaggaaacg
     4981 gcgggtatgg aggtcctttg attattaggc cgacaaaaga taaacacaag ttcatctatg
     5041 tcacaggtgg aggtgagagg ccaatgatcg tagataaaat agaagaactg acaggtatgg
     5101 agtctatcaa tgggttcaaa acatcaatac ctgatagtga aatcgcgctt gcaatcgttg
     5161 actgtggagg gactcttaga tgtggaattt atcctaaaaa agggatacca accatcaata
     5221 tcgttcccac aggaaaaagt ggcccattag cacaatatat cactgaagat atttatgttt
     5281 cagcagtagg gttagaacaa atacagccac taatggaaac tgatgataac aaagaaacag
     5341 taaatactgc atctccagat aaaaagtaca aatatgatac ttctaaaaag ataaccgagc
     5401 aaaaagcaga gcagcaaaac tggatagcaa aaattggaat gggcgcggga aaagtagttg
     5461 ctacattcaa tcaatcagca agagaagcag tagatacgat gatccggaca gtgatccctt
     5521 ttatggcttt cgtttccctt ttgattggtg tgatacaagg ttctgggatc ggtaatgtat
     5581 ttgctaacat tatggcacct ttagcaggaa atgcagttgg attaacaatc attggcttta
     5641 tttgttcact gccattttta tctcctttgc ttggtccagg ggcagttatt agccaagtga
     5701 ttggaacatt gatcggtgta gagattggga aaggaaatat tcctccccaa ttagcattac
     5761 cggcactatt tgctatcaat actcaaaatg catgtgattt tattccagtc ggtctaggtt
     5821 tggaagaagc aaaagctgag acagtagaag taggtgtacc atcagttctt tattcaagat
     5881 ttcttaacgg catcccacga gttatcattg cttgggtagc aagttttgga ttatatagct
     5941 g
//
LOCUS       DACDKL010000142         5923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDKL010000142 DACDKL010000000
VERSION     DACDKL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.144"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..2063
                     /locus_tag="IV329_002143"
     CDS             156..2063
                     /locus_tag="IV329_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8941309.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2604..5882)
                     /locus_tag="IV329_002144"
     CDS             complement(2604..5882)
                     /locus_tag="IV329_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8941310.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1696 a         1224 c         1129 g         1874 t
ORIGIN      
        1 agttgggatc gcctcctttt tttcattata agtgaaggat tgaaaaaaac aaatcagttt
       61 gattgttcga attttcaatt ttttatgttc tatttgttaa ttccttcatt ttgtaagata
      121 atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa caaagaacga ttgaaacaag
      181 atttctatga agcagtcaat gaagaatggc aaaaaacagc aaaaatccct gcagataaac
      241 cagctacagg cggtttccaa gatttagttg acgggatcga ccaacttttg atgggagaaa
      301 cagacgatct gcttgcccat ccagaaaaag tgtcttcaga aaaaatgaaa catttccttg
      361 catattatca actggcaaac gattatgacc gaagaaatga gttgcatgct caaccgcttc
      421 aacctataat cgaaaaagtt gaatcattgc agtctttcga cgaattgaat aagcaatttc
      481 cagaatggct gttggataca ttaccattgc catttaatct ggatgtagat gcagatatga
      541 agaatgcgca aacgaacgcc ttctttgctt atccgccttc tttattcctt ccagataaga
      601 cgtattatgc atcagaccat cccaacggtg cacaactaat aggcgttttc tttgatatgg
      661 ctgttcagtt attagagctt gccggcaaag aaaaaagtca ggcagaagca atcacggaac
      721 aagcagtgca atttgataaa ttgattgctc ctcatgtaaa aagtgcagaa gaaaacgcag
      781 attacagcaa aatgtataat ccgcaatctt ttgatacatt tattaaatac acagataaac
      841 tggacttgaa aaaacttgtc acgggtttga ttgggacaac tccagataaa gtgatcgtaa
      901 ctgatccagt gtacttcgat catttgaatg acatcctgac agaagctcat tttccgttat
      961 tcaaaagctg gatgatcatt aaaacagtac gttctcttag tggttatcta tcagaagaat
     1021 tccgccaagt cagcggaatc tattctcgcg tattgtctgg aacagacgaa gcaatgcctc
     1081 agaaaaaagc agcgtattac cttgcctctg gacaattcga tcaagtagtt ggcgattact
     1141 acggaaaaaa atattttggc gaagctgcta aaaaagacgt agaacaaatg gtgaaaaaaa
     1201 tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg gcttggggat gcaacaaaag
     1261 aaaaagccgt cataaaatta aataacttag gtatccaagt aggctacccc gataagatcc
     1321 cagcattatt cgaccagttc aaaacagtac ctgcagatca gggtggtact ttgctttcaa
     1381 atactcttgt tttcagcaaa ctcacattaa aagatcgttt cagcaaatgg aacaaaccag
     1441 tggatcgttc agaatgggaa atgagtgctg acacggtcaa tgcttattat catccatttc
     1501 ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc attttacagt ctagaacaat
     1561 caagctctgc taattatggt ggaatcggag ctgttatcgc tcatgagatt tcccatgcat
     1621 ttgataataa cggcgcttta tttgacgaat atggcaactt gaacaactgg tggacagaag
     1681 aagatcttac acaatttcaa aacaaagcac aagcaatgat cgaagaattc gatggtctgc
     1741 cttttgctga cggaaaagta aacggaaaat taaccgtttc ggaaaatatt gctgatgcag
     1801 gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc agatctttct atcgaggaat
     1861 tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa aaaagaatac caacaacttt
     1921 tactgcaaat cgatgtccat gcccctgcta aactccgagc aaacgtgcaa ccaaaaaacc
     1981 ttgacgaatt ctatgaaaca tttggtatca cggaacaaga cgctatgtat ctagcgccag
     2041 aaaaaagagt gcgaatctgg taagaggttg tgccagaagt ggtcagcttc aagaaataag
     2101 aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat ttcggcttat ttttcgaaga
     2161 agttacttct ggaataccat gtattagagg ttgtgaaaaa gctgcttagt ttcaagtagt
     2221 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact tactaccgag
     2281 aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa tcagcgtcct gacctgagta
     2341 ttagagaaaa aaacggaaaa atagttcctc atattttgcc gtttgttgag ctaataccga
     2401 gggtcagctg attgaacacc gtttagtgga gagattgtga aaaagctgtc ctgcatcaag
     2461 taataagaac agccaatcaa aagcagcaag gagaacctca tctataaaaa gatagaagga
     2521 agtgagacat ctattggtct catttccttc tatttttact ttcctcagtc aattgtttta
     2581 catcataaaa ggagaaattc cacttatttt tttcgctgtt tcatgacata aacaactaca
     2641 ccgattaaca aaattcctcc agctaatgct agcaacgggt tattaaattc tcccgttttt
     2701 gggagtcctt gagtgccatt tttttcactt tcttttgctg tcctatccgg atgagaaggt
     2761 tttccatttg gttttccatt tggtgattca tttcctggat tttctctttc tggtttgtca
     2821 tttcccgggg gcacatcggc agtttctcgc acagtgatcg ctttttgctg ggtaacagat
     2881 tctgtatgag cagaccattt ttccccatca aatatttccg aacgataagt gacttctagc
     2941 gtatattctc cttttgaagg tgctgaaaaa atagcggtat gattagaaaa ttgagcactt
     3001 gactgtgttt caacgatttg gatcttttct gggaaataac gcatatcttc aaagacaggc
     3061 gttccttgat ttggtgtgaa agttgacaca aaaactgtaa aaggcttcag ttcaatgata
     3121 tttccttctg cattctccgc cacaccattt gcttcttgta taataatcgg attattttgt
     3181 tcatttggtt ttgtctgatt tggttcttgt acgattgcat actctggata ttccgaagac
     3241 ggtgaggttt ctccgtcaaa aatagcacct acagataatg gaagaccatc cactctttcc
     3301 ttttctgttt gacgattccc cggtttaatc tgcatcagtc catgaacatg gatacttcca
     3361 ttttcatagc gcgtaacagg ctgctgacct tctttcgaac atgtatttag agactcgatc
     3421 caatcttttg atatggtatg tccaagagta ttttctgatt gtttctctcg ttcattccaa
     3481 tagtagttca acgagtggta gatttgatct tcgttttgat tcaatgggcg gatctgctct
     3541 ctcatctcaa gattctctgt tcggaaagat aagataccag aagcatggta gtctgtgttt
     3601 cctgctgaag aggtataaag agccacatta tatgaaccgt tattaaaact tgtactgcta
     3661 ttgacgataa tatctgttcc gctattggaa tcgattcctt tcgcaagatt ttcgaaagac
     3721 aaactattga tcaattcatg agggcccgtt aaagaagaac cgcccatttt gaatccatta
     3781 ccgttacctt ctccctcaat tcctggtacc cagccgtttc gataagcaac tgagtttttg
     3841 atgattactt ttccgatcga acctgtttca tttttagcaa aaaggtccca accgtcatct
     3901 gcattatgat acgcgataca accgtcaaat acatttcctt ctcctattgt cagtttggcc
     3961 gcaaatccat cggcatcttc aaatcctgga tcggcatttt caaaagatgt acagttcttg
     4021 actaagttat gcgcgggcca tgcttcgaaa ggatcatttg agctaccgct gatttggatt
     4081 cctgtatttc cattccgata agcatgaatc tcttcgatca aattatgatt tccagaaatc
     4141 tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa accctcgtaa accccagtag
     4201 tgactgaaca gtgtcatccc atttccagtt cctttaaaat caagtacagg acgcttgttt
     4261 tctggatttt tctctgcaat caactgaatc gggttttctc ttgtcccgtt aacgcctttt
     4321 ggaatcgtca atcctctttc gaaggaataa gttccaggcg ccatgacgat tgtctgccca
     4381 gcatatgcgt atctcaatgc ttgtgtcaga gataatggat tttgtctgct tccgtttcct
     4441 gcatctgacc cttctggtgt tacataaact gtcgaatatt taggtttacg gtagtccact
     4501 gtatgaagaa tctcttttgt ttcataacta gtcatctcta cgtattcttc tggcggatag
     4561 tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc cttccatcag ttttgtctga
     4621 taatcaaatt cccgattcgc agtgatagcc aagttttttg cgttcttaag cacctctcct
     4681 gttgctttat ttttaattgt caataatccg tcagcatttg ctctaaagga catagtatga
     4741 tcttggtttc ctgaatgatt actagaggtc acagtgtaaa tcggatcaat caagcgagta
     4801 ggtctttctt gcccaggagg atctgtcgca ggatctgagt aagcaacttc gattccttct
     4861 accttgatcg tcatattcct tgctgcagca aatcctgcat aaataaccga ttcatctgaa
     4921 gcgtataatt tttcccagtc atacatgatg tcagaagcca tttcttctcc tgtttcagga
     4981 tcgtaataat atgactctaa accagtattc gtttttttca tctctacgtc cagtttagta
     5041 atcgagttcg tggccggaaa caaattccca tgaccgtttc cgaatatatt ataagaacct
     5101 gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg ttgtttccac tgagacactc
     5161 ccaggagctg gcgcagcttg cggatcttca gatgatattc ctgtgattgc ccgtgtcccc
     5221 aatccaagtc gcatcgtata tttgctgccg ctcgtgttga ccacttcttg tctatccgag
     5281 tcccagacat attcgatccg actgcctagt atggcaaatg aatttgaata aaatgcctgc
     5341 ccaaacaaat gattttttgg tactgcgtct cttaccatta aagcaaatcc ttcttgggca
     5401 ttcgtatagg tccaagattc tatttcgatc gtcgcccgca tcctaaagtt gatgtctttg
     5461 gggattgctt gataataata ggtcattcca tctgatccgc ttgactggaa ttttccgccg
     5521 ttattcgtgg aagagagccg cacgccgtct tctaatggtt caatggcata cctactagca
     5581 cttgtgcttt ctccgaaaac aacccctttc cactcttcat ttgcttcttc agattgcttt
     5641 tttgtctttt cattttctga ttctttcatc ataggatcat ttccttcttc cgtatccata
     5701 tcttcactag ttcttggtac ttcctccatt gttgacatcc cttcaatgtt cacgtgtgca
     5761 ttttcttcgt tcacagactg ttgttcgtta gcagcaactt caacgacaga aattaaaacc
     5821 ggggtactga gcataaggag attcatcatt agcgcactgt attttatacg tttttttaac
     5881 aagtgtattc ctcccgtaac aaattagaaa gcgtttactt aaa
//
LOCUS       DACDKL010000143         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDKL010000143 DACDKL010000000
VERSION     DACDKL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.145"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IV329_002145"
     CDS             complement(<1..985)
                     /locus_tag="IV329_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP8941311.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IV329_002146"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IV329_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8941312.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IV329_002147"
     CDS             1810..2964
                     /locus_tag="IV329_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8941313.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IV329_002148"
     CDS             3179..4147
                     /locus_tag="IV329_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8941314.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IV329_002149"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IV329_002149"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8941315.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="IV329_002150"
     CDS             5177..5785
                     /locus_tag="IV329_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8941316.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DACDKL010000144         5879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDKL010000144 DACDKL010000000
VERSION     DACDKL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.146"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1183
                     /locus_tag="IV329_002151"
     CDS             <1..1183
                     /locus_tag="IV329_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8941317.1"
                     /translation="RSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQAIINNVDFVLLAG
                     DNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERYWFSFPKNVHLFTS
                     ETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYHIGLYHGEPGVSQK
                     GNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGHTKKEQASTSILLV
                     ELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSKVEDGFSLIEIKLK
                     DYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTFYTKTPLAASAKLM
                     EQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAKQLLNQDFLFEEDQ
                     E"
     gene            1180..3900
                     /locus_tag="IV329_002152"
     CDS             1180..3900
                     /locus_tag="IV329_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8941318.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3884..4828
                     /locus_tag="IV329_002153"
     CDS             3884..4828
                     /locus_tag="IV329_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8941319.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4897..>5879)
                     /locus_tag="IV329_002154"
     CDS             complement(4897..>5879)
                     /locus_tag="IV329_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8941320.1"
                     /translation="TSALAVLTLAACSGDTNKDIATMKGGTITVSDFYDEAKLESSNQ
                     SLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAGYTKDTYKEYI
                     RNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDVKKSADDGDDF
                     SKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDVITTTNPTSYA
                     TEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEELKDANVKIKDD
                     AFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDSSK"
BASE COUNT         2051 a          986 c         1148 g         1694 t
ORIGIN      
        1 tcggtcattt gaaggactga caactcttga taaaactgta caggaaaaac tattgaagac
       61 gaatctgacc gttttatcaa atatcattga acaggcaata ataaataacg ttgattttgt
      121 tttacttgca ggagataatt ttcatcaaaa tcgtccttca ttaaaaatac agaaacattt
      181 ttcagaacag atgaaacggt tagaaaagaa ccatatacct gtatttataa tattcggaaa
      241 tcatgatttc tatcaaaaag agcggtattg gttctccttt cctaaaaatg tccatttatt
      301 tacatccgaa acggtagaga caaaaaaaat aacgataaaa agtggagaaa ctgtctctct
      361 cagtggattt agttatcgtc aaccgtggat tcaaaaagac aaagtgatgg aatttccttc
      421 tagagaactc acagattacc atattgggtt gtaccacggg gaacctggcg tctctcaaaa
      481 aggaaactat gctcctttcc aaccctcaaa gatgcaagaa aagggatacg attattgggc
      541 tttagggcat atccatgtgc caacggtctt aaatgaaaag caaacaattg tttatccagg
      601 tgcgcctcaa ggtcatacta aaaaggaaca agcttctaca tctattctac tagttgaatt
      661 aagtaaagga agttgccaaa tacatccgat taaagtagca gaggtctatt ggaagacaaa
      721 ggaaatatca ttgaggaaag caagaacgac aaaagaggtc attgttcaca ttcgtcagca
      781 gctttcaaaa gtagaggatg gatttagttt aattgagata aaattaaaag attatgacca
      841 tctcaataca gatgttttgg aacggataca atcaggagag ctattagact atttgttaga
      901 ggaattctca gataggataa atgatttctt tatttggcgt atttctctca tagagaatac
      961 tttttatacg aagaccccac ttgctgcttc tgctaaattg atggaacagt tatttcaata
     1021 ctatcagaca cctcaagatt tccaacaaat tttaaatgaa gtatatagtc atcaagaagc
     1081 ggctagaata ttgagcgaac tgccggaata tcaagaagaa acacttgaaa aagccaagca
     1141 gctgcttaat caagattttt tatttgagga ggatcaggaa tgaagatttt aaggatcgag
     1201 attgctgctt ttggaaaatg gcgtcaaaaa agttttgact tttattcagg caatcagctg
     1261 atctatgggg gaaacgaagc aggaaaatca acgatttatc aatttattca agcaatatta
     1321 tttggtttcc cttcaaaagg ccgtaaaaaa aaggattata caccaaaaga cggctcagcc
     1381 tatggcggca aaatatggct taaacatcct gtatatggtg aatttgctgt cgagcgctac
     1441 aagcagcaaa atcgcggaaa atcgaaggtg tggctggggg atcaagtggg aagtgatgag
     1501 ttactagaaa aactgatcgc acctttgacg aaagagttat tccagcaggt atttactttc
     1561 cagcaagagc agttgatgga actagatcat ttgagagaaa aagagctaca tgatgcgctg
     1621 atttctttag gcatcacggg cagttctcaa gtattccaaa agcggcaaga atattatcag
     1681 aaagcacaac aattattcaa gaaaaaaggc caaaaattac taatcaatca aaaattaaat
     1741 gaatggaaag agctgcaaga aaaaattcat caaaaacaag aacaggaagc ttattttggc
     1801 aagttgatcc aacaagaaca ggaatacgag tcgcaacagc agcagttatt ggcacaatta
     1861 aaagaggcga acaaacaatt ttcattagcc agacagcaag aacttaattt ttctttatat
     1921 gaagaatggc aatctctcaa aaaaaacagc actacaggtc ttcctgatga gcaacttctt
     1981 ttagatctgg agtctttctc caaacggtat cagcaaacaa atgagcgttt ggaagaatta
     2041 gaagcaacac tggaaaagca tagcgggatg gatcagcagt cagccagata ttatttttat
     2101 ttagagcagg agcaggaact aaaagatctc cagcaacttc aatttacagc ggaacaatta
     2161 aacgaagaat ggcaaagatt acaaattgag actagagaaa caaattcaga attgaagatt
     2221 ttggaacaaa ggtggcattg gcagaaaaat ccgttaccgg aagaattttt atatgaagaa
     2281 gaatggaagc atcttctggc tgaagaaaga aggattgctg atctagaaag ccagatttct
     2341 gtgcgtctcc aaatagctaa agaacagcaa gagatgattg aagcagaagt gaatgacttg
     2401 gaagagacct atccagcatt attacaaaat cagtcagaga tagcaccagt ctcttcatct
     2461 aaaccgtata tgctgatggc tggcggagca ataatcattg caggtatatt tgtaccaatg
     2521 cccttgaaag tgatcctttt actaattggt gcaggcatga ttggctattt cttctacaaa
     2581 aggcaaccaa aaattaaaac taaaaatgat ttctctgaga taaaaggaat gtggcaagaa
     2641 aaactaggac aacttgatct agccttagct caattagctg aagcagaaga aaaacaaaag
     2701 agaactggag aagaaaaaca gattcttcag caagaagtat atcgttttgc ggatcatcgg
     2761 cacttaggaa agatggatac gctcaaactc atgaaagaat atggacagga agtcatagat
     2821 tatcagaaat tatatggcgt atttgagcgg aaacataaaa gagaacagga aatacaagaa
     2881 aaactacgac aaatcgacca aaaatttgag tttgttaaag aatggttacc gatacaagat
     2941 aagacaattt cagaaaaaat ggtattgctt tcccgtttta tacaagagat ggaacagatg
     3001 aaatttgcca gaagctatca gcaaaatact ttattgaagc aagaaatcaa tcaattgaaa
     3061 aagaaacaaa aagtatggat acaagaatac agcgaacaat tgactcaagc aggcttatct
     3121 tatccttcgg aagtacctgt ctttttacaa aaagccaaac aagccgaaca gcagatgaaa
     3181 agatacaatg aattatcgga aatgcttact ccactatttc ctgaagagct gacaaaagag
     3241 aaattatctg taagactgcg ccaactgaaa gaaaagcaat cccaactcca gcaagaagaa
     3301 aaagaagcaa gtgaaaatcg gcaacgactc caacttcaag tagaagagtt gcaaagagac
     3361 ggcacattgg acgaacttta tcaacaagct agccggcaaa aagcaaaatt acaggaatta
     3421 ctggaggaat ggggagcact tgaaatcgcc gggacattct taggtgatct ttcaacagag
     3481 atgagtgaaa gacaattacc ccagttgtta agagcagcag gtgattattt aggtatttta
     3541 acaaaaggaa attatcagaa gatattactg aaaaatgata cactgacttt aacagacgga
     3601 tatacagaat ttcccatcta tgagttatct acaggtacga aagatcagct gattatggcg
     3661 atgagatttg cttacctctc attagaagct aaccgttcga tatgcccgat catcattgat
     3721 gatggctggc ttcattatga tcatcagcga aagtatcaat tagctgaact tttagctaaa
     3781 tttggagaaa agtatcaaat aatttgtttt tcttctgata aagaaatggt aagctattac
     3841 catgagatga accaaatcgt aaaacaatta gaaggagcaa ataatgaaaa aaattcgtga
     3901 tttagtaatc gatgagcagt ttgaacaatt tgtcctgatc aaaagtgcag atgtaagaat
     3961 cgctaaaaac ggaaaaaaat ttattgcttt tactttccaa gatacttcag gtactatcga
     4021 tggtaaatac tgggatgctt cggaggagga aatcaataaa tttacagccg gcacagtcgt
     4081 ttttctaaac ggtaaaagag aggtttatca aggaaaccct caagtaaaga ttcttcatat
     4141 gcgcttaaca agggtagatg agccaggaga gcctagttta tacatggaaa gagccccttt
     4201 gaagaaagaa gaaatggaag aagaaatcaa tcaattgttg tttgagatca ccaatgcgca
     4261 ttggaatcgt attgttcgct atctacttaa taaatatcaa aaacaatttt ttgagtttcc
     4321 ggcagcaaaa agaaaccatc atgcatttgc cagtggatta gcttatcata ctttgacaat
     4381 gcttcactta gggaaatcaa tcgccgatga atatcctgag ctgaatcgag cattgttgta
     4441 tgctggtatc attttgcatg atttaggaaa ggtgattgaa ctttccggtc ctatgtcgac
     4501 agaatatacg cttgcaggaa atttgatcgg tcatctggta ttgattgacg aagaaataac
     4561 taaagcctgt cttgcgttga aaatcagcga agctgacgaa gatgttcttg tattacgtca
     4621 tatggtttta gcacaccatg gcttattaga atatggctca cctgtacgtc caagaataat
     4681 ggaagcagaa atattacatc aaatcgacaa tatcgatgct tcgatgcaga tgatgttgac
     4741 ttcgattcgt caaacagaac caggaaaata caccgatcgg attttcggta tggacaaccg
     4801 aagtttctat gtaccaaaag atttatagca aaaatttatt tttttcattc aaaacaaaaa
     4861 aagcgagaca taagtcttcg ctttttttgt tttcttttat tttgatgaat cagttgtttc
     4921 ttcagttgaa gattcttttg ttgaatcagt tgattttgtg tcagaactct ttgttgaagc
     4981 tgtagtttca cttgagtctt tcgtacttga tgaagttgtt gtgaatgcac tcaacacatt
     5041 ttcaaaggca tcatctttga tttttacatt tgcatctttt aattcttcac cgattacttt
     5101 agttgtgaat gcattgtcac ttagtttagt ttcagtagcg atatctttta gttgatcttt
     5161 gtatttatcc atgtcattgc ctttattttg gtttttcacc attttgacaa cgtagtattc
     5221 tgtcgcataa gaagtaggat ttgttgtagt aataacatcg gagatctcac cgtctttcaa
     5281 tttgaaagct gcttctttta cttctgcagg aatagttgtt gtcgttgagt cgaatttaac
     5341 tttaccgcca tcttcttttg tctctgtatc agttgatttg tctttggcta atttagagaa
     5401 atcatctcca tcatccgctg attttttcac atcttttgct tcatcttcac ttgataattt
     5461 gatgatttgt gcttctactt ctggatggaa agatttccaa gcagttttca aatcgtcatc
     5521 tgtaatatct acgtgagctt tcaagccagc ttcaaaagct aaattgttac gaatatattc
     5581 tttataagta tctttcgtat atccagcagc ttctaattgt gattcaaaag tatcacctaa
     5641 tgatttcgct tgtttgtcgt attctgcatc cacttgctta tcggttactt tatcgccata
     5701 tttgttattg aatactttat aaatgatcat ccgttgtact aaggattggt tcgatgattc
     5761 aagttttgct tcatcataga aatcagatac agtgatcgtt ccgcctttca tcgttgcgat
     5821 atctttatta gtatcgcctg aacaagctgc tagagttaaa acagccaacg cactagtag
//
LOCUS       DACDKL010000145         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDKL010000145 DACDKL010000000
VERSION     DACDKL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.147"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IV329_002155"
     CDS             450..1604
                     /locus_tag="IV329_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8941321.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IV329_002156"
     CDS             1598..2020
                     /locus_tag="IV329_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941322.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IV329_002157"
     CDS             3143..4075
                     /locus_tag="IV329_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8941323.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IV329_002158"
     CDS             4092..4607
                     /locus_tag="IV329_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8941324.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IV329_002159"
     CDS             4903..5184
                     /locus_tag="IV329_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941325.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IV329_002160"
     CDS             5186..5602
                     /locus_tag="IV329_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941326.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDKL010000146         5783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDKL010000146 DACDKL010000000
VERSION     DACDKL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.148"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(97..1227)
                     /locus_tag="IV329_002161"
     CDS             complement(97..1227)
                     /locus_tag="IV329_002161"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8941327.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1875..3077)
                     /locus_tag="IV329_002162"
     CDS             complement(1875..3077)
                     /locus_tag="IV329_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8941328.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3137..3340)
                     /locus_tag="IV329_002163"
     CDS             complement(3137..3340)
                     /locus_tag="IV329_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8941329.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3482..4714)
                     /locus_tag="IV329_002164"
                     /pseudo
     CDS             complement(3482..4714)
                     /locus_tag="IV329_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4877..5689
                     /locus_tag="IV329_002165"
     CDS             4877..5689
                     /locus_tag="IV329_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941330.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1714 a         1189 c          920 g         1960 t
ORIGIN      
        1 ataaataata taaaaggtaa ataaaaaaga gtggatttct ccactccatg aaaagccgac
       61 tttttgggac gctttttttt gccaagcctc acatttttat agccaaactt cttttgcgac
      121 ttgctgcaat aattggattt tcttccattg ttcttcttct gttaaatggt ttccttcttc
      181 tgttgaagca aacccacatt gaggactgat acttagattt tccaatggta caaattgttg
      241 tgcttctttt attcgttctt ttattttctc cgtatcttcc aaatctggaa acttagaagt
      301 aactaatcct aaaacaactt tactttcttt gttactccaa tgtcttaaag gagtaaaatc
      361 tcctgctcgg tcagaatcgt attccaaaaa gaaaccatca taatttgttg aaaacaactc
      421 ttctgcaatg ggttcatatc caccggaaaa cagccaagaa gaagcgtgat tcccgcggca
      481 gatatgtgtt gtgatcgtca aatcttctgg tttgtcttcc agtgcgttat tgataatctc
      541 tgtacaaata tgggccattt ctttcggatc aattcctttt tgaagaagtg cttctctttt
      601 agtttgatca gctagaaacg cccaatttgt atcatcgaat tgcaggtaac ggcaaccttt
      661 ttcataaaaa gcatgaatcg ctgctttgta agtagaggac aagtcctcat aaaaatcaga
      721 tagatcagga taaatctcct tgatttttga agaaccatca ttagatagga tctcttgtcg
      781 taaaatcatc gtgggactag ggatcgttgc ttttgcaaca aaagtatcat ctgtttccac
      841 tacttctttc agaaaagcaa aatcttttaa aaatgggtga tttggattga aagataattt
      901 atctactacc cgaatattat aagctggtgc tgcttttccg tggaatgttt gattatatcc
      961 agtttctgga acatagcctt cgataccatt tagttcttct aaaaaatcaa gatgccaaaa
     1021 tccacgccga aattcaccat ctgtaattcc tttgatgccg actgcttttt ggtcttccac
     1081 taattttttt atttcctggt cttcgattgc acgaagttct tcttttgaaa gtaagccttc
     1141 ttgaactttt tttcttgctt ctttgatcga ttgcgggcgt aagaaacttc cgacatgatc
     1201 tactgtaaat ggtctatttt tgctcatttc actcactctt ttctttgttt tttgtaaaaa
     1261 aaatcctttt actagcaaat actagtaaaa ggacgattat actcgtggta ccaccttttt
     1321 tcagaaaaaa cattctttct ctcagcctga caataaaatc agtggtcttg gtaacgaaag
     1381 actgttcgtt tttgcctacc tttcgacaaa aaatcctcta aggccatttt cgacagtcaa
     1441 cttctagctt tttacaccaa ccaaagcctc tcttgaacca ttttctgtct actttccttc
     1501 tcatcggata ctattagaat aaaattagaa tagcatgttt gaaagaaaag tcaataggga
     1561 ataagaacaa acaaaaacta atttgttatt cttagtaatt tgtttgtgga atacaataaa
     1621 gcttttctaa tatgataatt gttcagtcgt cctttcatct tagatcactt ccttaaaatg
     1681 atcatacccg tttcaaccac ataaaaaagg acttaatttt gaaaaatttc ccatcaaaac
     1741 taagtccttt ttattatagt tatgcaaatt tgcttatttt ctcgctattt ccgatacatc
     1801 ttgaacagga gcaattcgac tgaaaacact ggtgcaaaag catttatttg tcttatggta
     1861 gaaccgtgga aaactcagac tggtaataaa ttgtcatttt ttgaaagaat agcgccctct
     1921 tgaacattat caatcgcact taccacttgt tgatctgact ttgcatataa gtgaccgtaa
     1981 gttcctaaag tgatttgaat attcgcatgg cccaatcgtt cttgaataga tagcggttga
     2041 atattctgct caattagaaa tgcaacatga gaatgtcgta gcgcgtgaag cttaatcggt
     2101 ttgacacccg cttttttagc agcttctttt aaccagttcg caaaagaata aggtggcgta
     2161 aatgttccgt ccaactgagc aacatattca aaatttccaa tacgtttttg caagtcatac
     2221 cactgtctga gttttaagga aagcttcttc cccaacccaa tcgttcgata agcacattta
     2281 gttttgggag tcgaaaagta ccaatcatct tttgtattat aaatcaatgt cttctcaatc
     2341 gttatcgtgt ttttctcaaa atctacattc gaccaagaca aagcttcaag ttctccaatc
     2401 cgaatgccag tataaaagag catttcatac acaatcttcc gatggtgggt ttgaatctta
     2461 tcatccttca attcattcat cactttttga aactcactgt gttcccaaaa ctcaactttc
     2521 ttattctcag aaggaagcga agaaacatct tcaacgggat ttttagcaat caccccctct
     2581 ttaacagccc gatccaatac aatttttagg tgccctcgaa tccgattgat ataagatttt
     2641 gataacgttt cgccattaac ttttttccgt gaattacgtg actctgtctg gcttagttca
     2701 aataaccaat cctcaatatc gtgacttgta atatctcgaa cacgaaaatg atagaaaaac
     2761 tgcatacgct tttcgaaaat gaattgcgca ttctcatatg tttgactttt gacctgtctc
     2821 tcgtaccatt ttacaaacct atccttgtaa aactcttcga aagtcatttg attggtagaa
     2881 cacagttgat taggaattgt cttgttaaaa tcatgtgttt ccatgtactt gtcataagca
     2941 attttggcat ctttttgtga agcataacct cgtttagtct tctgaacctt tcttccattt
     3001 tcatctatac ctagatacgc tcggaagaac catttttttg tctttttatc ttggtaaata
     3061 tttttaattt ttggcatcgt tccattcctc cctgtacttg agaaaagcta ctttaatagt
     3121 accgcgcttg tactgtttat tccgcaactt gaatgtgaaa aagatgttca attactcggg
     3181 atggcaccac accaacttgg cgattattat aaaaatcaat accttcaatc cttgcaagat
     3241 atgccttgct ctctttaatc attcgtgcag cttgactagg tttaaagccc atctctatca
     3301 aaattgcacg atttaccatg aaatcacatt ttgtttccat tctctcacct cctgattctt
     3361 tactcctttt aaaataatcc tctttataat ctattgtttc tcattcgatg gttgtgttat
     3421 actcgtcttg tcgaggcgga gtacgttgca caacttgttg tgtaagcagt actttgcttt
     3481 tttagtagct tgttttctca tttgatatac tttgtaattt tcgttcataa tactcaacct
     3541 cctcctgata atactccggg tctgccttca tattttggag aagtttgatc tgtcttggat
     3601 tcatttctcc ttgttcaata atcaaaggca acaaggaaga atgaaacgtt tgatcgacct
     3661 gtgcaataaa aactaatgat ggtgaaacac catgaattaa ccaactcata gttttctcaa
     3721 aactttgagg aattgtatct aaagaaatgg tgattgcgcc ataattgcta ataaaggctg
     3781 cccatttggc atcaactttt gcacctagat catttggatc acggtcataa aaacaaattg
     3841 ctccattaag taactcaaag aacagtttgg ttaaatcatg ggtttttgaa ataatctttg
     3901 ccaagctttg cgctttctct tttcgatacc gtagttcaaa gcgattcttt acttttgcct
     3961 cttctaaagg aattcctcgt cgcttccgtt gttcatggtc cttctgataa aagcaaaaac
     4021 gacactgact cttctttgag cctaaataca aggtctcacc atttgattca ttgtttatga
     4081 tatccgtacc gccatgagat tcactcactt gaaaagtggt ccacacatgg cctagtatta
     4141 ccttatgctt aagttctgga atgctgagac tccccacaaa atcatctaaa gtaaaatcaa
     4201 tccgtgtaaa gttcccctga taatctaata ccttacgaaa aaactgttgc caatttgttt
     4261 ttcgtgtttt tagccatctt gctaagtgtt tacagccttg acctgaaaac tccatcacag
     4321 ttcctttaac cgcatcgtca atggaatacc gaatcgtgat tacttgattc cacacgtatt
     4381 ggccaatata gccctttggc gcataatcca gttcctcaaa acgttcaatg ggcatttcaa
     4441 taatctcttc aattagcttt aaatgttgat tcgagaaaaa gtgaatggtt agataatcaa
     4501 tcaaaacacg gttaactgga acgacctctt cttgcaaact atccaacaca agattcaatt
     4561 tttcttcact taattgtctc gttccttttt ctactttcga taataactat ctcgaaatcc
     4621 ccactttttc tgctaaatat cgttgtgtcc accccctttc ttctcgagaa actcgaatgg
     4681 cttcaccgag atcatgttga ttaacttggt tcatcgacaa acctccttct gctatttact
     4741 tgtcaaagaa cctattgaac ttattttaag tacattttta ttgtacgcct gtatttattt
     4801 taagtcaata tgttttgact ttttttaagt acacacgtat actaaattta agaacttatc
     4861 ctgaggagga gttttcatgg agatagacaa gcaagcagtc ggcaatcgaa ttcggcagat
     4921 tcgccaagaa ttaaagttga gtatggagaa atttggcaaa ctaattggtg atttaccgcg
     4981 aagtacagtc aataactggg aacgtgggat caaccttcca aaaaccgaaa cccttcacca
     5041 aatcgcagag ataggtcatg tcaccaatga atatcttttg tatggtgatc aagagaatca
     5101 atacatttta gaaatgctcc aaaaaaaggc aggtaagctt gatcctaaga ttgaaggtct
     5161 catagtagac gaaatcaaac acgctgacct tgttagtgaa aaagaaatgg atcgaatgat
     5221 tgaatttttt attacaaatc tcattccacc aactgaacaa gaccagttta ctttccaatg
     5281 tatcgatgaa aaaaagcagc tttatcttgg ttcaactaat tttggtaagg aagcccagct
     5341 ctatttgcaa catgataatc aaaatcatat tctgcacatg atgcccttta ccttctccaa
     5401 ctttagtgtg gatcgcatac tcgtctattt agcaaatcaa aaatcctatt cctattttgg
     5461 gaaacattta cccaaggaac taggtgagaa agccatcctt ctatactcaa ttaatcaatt
     5521 gaccagcgat gtgcgaattg ccccgttggt ctattcagaa gaaaccgctt cttatcagta
     5581 cacagaggac aatcaatatc tactagaaca gcaatatctt tatctaccat tcgtgatgga
     5641 agtcgagaaa gaacgcctgt taaatgcagc gtatccatca tcaaaatgag gtgacaaaat
     5701 tgataagcac gtgacaaaga aaatactgct aggacactcc ccaatgggta gttgtcacag
     5761 tttcctgttc tttgttagcc ccc
//
LOCUS       DACDKL010000147         5777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDKL010000147 DACDKL010000000
VERSION     DACDKL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.149"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(525..1028)
                     /locus_tag="IV329_002166"
     CDS             complement(525..1028)
                     /locus_tag="IV329_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8941331.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="IV329_002167"
     CDS             1387..2412
                     /locus_tag="IV329_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8941332.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="IV329_002168"
     CDS             2735..3613
                     /locus_tag="IV329_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941333.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="IV329_002169"
     CDS             4089..4205
                     /locus_tag="IV329_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8941334.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="IV329_002170"
     CDS             5133..5471
                     /locus_tag="IV329_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941335.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..>5777
                     /locus_tag="IV329_002171"
     CDS             5565..>5777
                     /locus_tag="IV329_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941336.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDY"
BASE COUNT         1966 a          875 c         1061 g         1875 t
ORIGIN      
        1 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattat
//
LOCUS       DACDKL010000148         5732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDKL010000148 DACDKL010000000
VERSION     DACDKL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.150"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            632..1882
                     /locus_tag="IV329_002172"
     CDS             632..1882
                     /locus_tag="IV329_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8941337.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            1901..2911
                     /locus_tag="IV329_002173"
     CDS             1901..2911
                     /locus_tag="IV329_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP8941338.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            2921..3574
                     /locus_tag="IV329_002174"
     CDS             2921..3574
                     /locus_tag="IV329_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8941339.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3593..4591
                     /locus_tag="IV329_002175"
     CDS             3593..4591
                     /locus_tag="IV329_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8941340.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            4667..5428
                     /locus_tag="IV329_002176"
     CDS             4667..5428
                     /locus_tag="IV329_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP8941341.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5446..>5732
                     /locus_tag="IV329_002177"
     CDS             5446..>5732
                     /locus_tag="IV329_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HAP8941342.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSV"
BASE COUNT         1709 a          919 c         1411 g         1693 t
ORIGIN      
        1 agaaaaaaat attatcaata gattttattt agttcagaat ttttacgtga aagtagatta
       61 ttaatgataa tctttatcca attagaatta ttatcgtagg atgtgatgat gaaagccgtg
      121 aaattgttca agtatttacc aatgtctccc tgccagtaaa tccgcttaga atttgagcct
      181 tgaaaatgtg caatgataaa aaacaaaaac tcgctcaaaa tagacagttt ggggtgggaa
      241 tagaccaata atgtaccaaa caaacttcca tttggggtga aaataggtca ataaagcgat
      301 gttaaacata cctttggagt aagaatagag ggtaataagt gaatttataa actttttggt
      361 gactggttga gtggaaaatg taggtacatt tcaatgcgaa aatgtattat aaccccaaac
      421 gaggtcaata aaaatgactc aaacttgcaa tataacccta aacgggtagg ggatttatga
      481 aaaattgaat ttgccggcgt aggagctggc tgttttaagt atgaatgaac acagctttcg
      541 ggctgtttta tataaatagc aggttactgt tcacaaacag cattttgtta agaaagtagt
      601 gaccacagca gtggtggaaa gaggaaatat tatgaagaaa cagtataaca ttgcagtcgc
      661 cggcaccggt tatgttggtt tgagtattgc cactctgttg gcacagcacc ataaggttac
      721 agcggtggat atcattccag agaaggttga actgattaat aaccgtaaat ctccaatcca
      781 ggatgattat attgaaaagt acttggctga gaaagaactg aatctgaccg caacattgga
      841 tgcaaaggca gcataccagt ctgcggattt tgttgttatt gcggctccta ctaactacaa
      901 tagccgcact caacattttg acacatccgc tgttgaggct gttattaggc tggtcatgga
      961 agtaaatcct gatgccatta tggttattaa gtccaccatt cctgttggat acaccgcttc
     1021 tgttcgtgta aagactggta gcaagaatat tattttcagc cctgagttcc tgcgtgaaag
     1081 taaggcactg tatgacaatc tgtatccttc gcgtattatt gtgggtaccg atatggatga
     1141 tgagcgtttg gttgaggatg cacacacttt tgctacactg ctacaggaag gcgcaatcaa
     1201 agagaatgtg gacacattgt tcatgggctt tactgaagca gaagctgtta agctgtttgc
     1261 caatacatat ctggctcttc gtgtcagcta cttcaatgag ctggacactt atgcagagat
     1321 gaaaggcttg aacactcagc agatcattaa tggagtttgc cttgatcctc gtattggtac
     1381 tcattacaac aacccttcct ttggttacgg tggctattgc ctgccgaagg ataccaagca
     1441 gcttctggcc aactacgcag atgttcctga aaatctgatc gaggcaatcg ttgaatctaa
     1501 ccgtactcgt aaggacttta ttgcggatcg tgtacttcag atggctggtt attacaatta
     1561 cgcagaagca aatcagtatg atgctggcaa agaaaagcct gttgtgattg gtgtttaccg
     1621 tttgaccatg aaatccaatt ctgataactt ccgccagagt tctatccagg gcgttatgaa
     1681 gcgtgtaaaa gctaaaggtg caactgttgt gatttatgag ccgaccttga aagatggaga
     1741 gactttcttt ggcagtaagg ttgtgaatga tttggaacag tttaaggcaa tgtgtgattc
     1801 tgttatagca aatcgctacg atgcttgcct tgatgacatt aaggaaaaag tatatacccg
     1861 tgatatcttc cagagagact aagattagga gagagtattt atgattatta caaaaacacc
     1921 gtttcgtatg tctttttttg gcggtggaac ggatatggag gattatttca aagagaatgg
     1981 cggtgcagtt ctttctacta cttttgataa gtactgttat gtgaatgtga gacatctgcc
     2041 tagatttttt gattacagcg ctgagttatc ttattctcgt actgagagag taactagcat
     2101 tgaagatatt gagcatcctg cgattcgtaa tgcgatgaaa atgctcgata tgcatgaaat
     2161 tcggcttaca tacgaagctg atttacctgc tcgctctggt ctgggtacat ccagttcttt
     2221 tgcagttggt atgttgaatg ctttttatgc actggagggt aagtatgctg acaagaagaa
     2281 acttgctgat gaagccattt atcttgaaag agttctgtgt aatgaagccg gtggatggca
     2341 ggatcagatt gccgcatctt atggtgggtt caatcgaatt aatttcaatg tggatggtta
     2401 tgaggtgctg cctgtcatta tttcgcctga acgtaaaaag cagcttaaca acaatctgat
     2461 gatgttcttt accggattca ctagattttc ttctgatgtt caaaaggcta acaacgtcag
     2521 tggcacagag gataagagag taagactgaa aaagatgtat gagcttgtag atgaagctga
     2581 ggcaattctt actgataaga acagaaattt agatgatttc ggtagacagc ttgatgttac
     2641 ttggagattg aaaaaaggta ctggtggagc aattagtaca ggaagcattg atgaactata
     2701 tgaaaagggt atggctgcag gtgctttagg cggaaagttg ctcggtgcag gtggtggagg
     2761 tttcttggtt ttctatgtac aacctgagaa acaggatgca gttcgttggg ctatgcgtga
     2821 tttgatgtat attccgtttg aatttgaaaa tggtggaact agagttattc actacacgcc
     2881 ggaaacttat attgaaaaac agaatggata aggagtaaca atgttagcgg aaaaacttaa
     2941 taaacatatt gacttattga ttaaacgata tcctacactt gagacgatta aacaggacat
     3001 tatcgatgga tacctaatta tggaggaatg ctatttgaat ggtggtaaac ttcttgtggc
     3061 aggaaacgga ggatcagccg ctgactcgga acatattgct ggcgagttga tgaagcggtt
     3121 taagacgcca cgtcctgtaa gtaaggaatt tgccgataag ttagttgcaa ttgaccctgt
     3181 aagaggtccg gaattagcca aaaatctcga gtgtggttta acggcaattc ctttggtcgc
     3241 acacgaagca ttgtctactg cttatattaa tgatgtcgat ggtttgggtg tcttcgcaca
     3301 gcagctgttt ggctttggta gatctggtga cgtattcctt ggtatttcga cgtccggaaa
     3361 tagcaagaat attatgagcg caactgttgt tgctagggct ctcggtataa aagttattgg
     3421 cttgacgggc gcaaagggtg gggaacttgc cacggtagca gatgtttgtg ttaaggctcc
     3481 tgaagttgaa acatacatga ttcaagagta tcacttgccg atatatcatt gttggtgttt
     3541 gatgctggaa gataaattct ttggaaacga ataaaatttg gaggaatgtt aaatgaaaaa
     3601 gacaattatg gttaccggtg gttgcggtat gattggctcc aatcttgtta agagactggt
     3661 gaaggaaggc tgctgggatg tttatgtagc tgataatctg tggagaggaa agttggagta
     3721 cctgaacgac gaagatgggc atcctgtcat cgacctggat actcatttct tcaatgctga
     3781 tctgactgat tatgagcagg ctaaaaaggt tattggtacg actgagtatg tagttcatct
     3841 ggctgatgtt gtggctggca ttgactatgt gtttaagaac cagggcgaat tgttccgaat
     3901 caataatctg atcaattcta atgtttttga ttgttgccgt aaggctggca aggagaaaat
     3961 caagggtgtt atctatgttg gtaccgtatg ctccttccct ctgaccagac agaatacttt
     4021 gaatcctgag cctctccgtg aagaggagtt gttccctgct cttcctgagt ctgcttatgg
     4081 ctggagcaaa ctgatgggcc agttggagat ggggtatttg gaaaaagaaa ccggaattcc
     4141 gtgctgtatc ctgatgctcc ataatgttta tggcacacct actgattttg gcgaacgtag
     4201 tcaggttatt cctgctctga ttcgtaaagc aatcaataat cctgaagagc cttttaatgt
     4261 gtggggtagc ggagagcagg gacgtgcttt cattcatgtc aatgatatcg taaatgcatt
     4321 ggtgctcgcc ctggataagg gttggggtca tggtcatatt cagattggcc cttctcactg
     4381 tacttccatc aaggaaattg catataagat cattgaaatt tctggcaaag acatcaagcc
     4441 tttctttgat acttctaagc ctgaaggtga taaggctcgt tgcgcagatt attctaaggc
     4501 gaaagagatt ttggattggg agcctactgt ttctttggaa gagggtcttc gtgaatccta
     4561 caattggatt gaaaagcaga ttaagggtta ataagtatat aagctgttct tcctccgtgt
     4621 aattagagga tgcggaggaa gaacatatga gtaaggatgt actgagatgg aaaagaatat
     4681 gtataaaaga aaacgtgatt tcactgtgtg tggaatagta ggtgtttgtg ctactacaaa
     4741 gaacgagttt gtaagggaag ttttttccat gccaacatct ccggtaagtg cgactgttga
     4801 tctcgtggga gtgccggcca ttatttcggc aaaggaaaat cccaaaattg ctgaaatgta
     4861 tagccaggct acaatggctg caatagatgg tatgcctatt gttaaaattg gacggcgcaa
     4921 aggatttacc tgtgaacgat gtgctgcacc agatattatg ggaatggttt ttgaagaagg
     4981 cgttagacag ggaaaaacac attatttcta tggtggaaaa gataacgaag tgttaaataa
     5041 gcttcgtgca aacctagaaa gagattaccc gggaatcaaa attgttggaa tgtattcccc
     5101 accatttcgt ccgttaaatg aagaagagga tagcaagatt tgcgaggaaa tcaacaactt
     5161 aaatccagat tttctgtggg ttggtattgg tgcgcctaag caagaaatgt ggatgtggga
     5221 acaccagaag aagatccata acacagtaat gttcggtgta ggcgccggct ttaacttttt
     5281 tgcgggcact ttggaaaaag ctcctgaatg gatggaaaaa gctagtctag agtggttatt
     5341 tagactggta aaggaacctc gtagactatg gaggcggtat atcttgggag gtataaagta
     5401 tatctactat actattgaat ctaagtaggg gggcaataaa aatagatgaa aatacttata
     5461 gtagttacaa tgacgaataa ctttggtaag aaggggtcct ataattctca ggaaatagga
     5521 ttagctcgag ggttagctga attaggccac gaagttattg tatataaaag tgtaaaaagt
     5581 gatgaggaag ttggaacaga gcaattaagc aatacggcga tgattcacta tatcaagtgt
     5641 cgttatgtag gatcaaatgg attcttatca acaaagaatt ttgataagaa tgcggatgtt
     5701 ctaatacaat ttgcggacct tcaactatca gt
//
LOCUS       DACDKL010000149         5681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDKL010000149 DACDKL010000000
VERSION     DACDKL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.151"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(73..1194)
                     /gene="mnmA"
                     /locus_tag="IV329_002178"
     CDS             complement(73..1194)
                     /gene="mnmA"
                     /locus_tag="IV329_002178"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8941343.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            1598..2197
                     /locus_tag="IV329_002179"
     CDS             1598..2197
                     /locus_tag="IV329_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8941344.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2335..3621)
                     /gene="efmA"
                     /locus_tag="IV329_002180"
     CDS             complement(2335..3621)
                     /gene="efmA"
                     /locus_tag="IV329_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8941345.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3892..4236)
                     /locus_tag="IV329_002181"
     CDS             complement(3892..4236)
                     /locus_tag="IV329_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8941346.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(4373..5509)
                     /locus_tag="IV329_002182"
     CDS             complement(4373..5509)
                     /locus_tag="IV329_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8941347.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT         1668 a         1232 c          991 g         1790 t
ORIGIN      
        1 aacagctatg ctatccaaaa gaaaaggtgt ttcttttgtt agccatagct gttttgattc
       61 atttaatatc gcttaaacgt attgtaaaac ttttgtttct tgatatgcgt ggtcgatcaa
      121 accaccaccc agacattcca ttccatcata aaatacaact gcttgtccag gagtgattgc
      181 acgtacaggt tcgtcgaata cgacttctgc acgattatca tccaataagc gaactgtcac
      241 aggaacgtct tgctgacgat aacggaattt cgctgtacac ttgaattctt ttggcattgg
      301 ttcattcgtc gtaaagtgga tttcacttgc atccaagctt gtggcataca atgcaggatg
      361 atggaaccct tgccccacat ataatgtgtt tgtcgctaga tcttttccta caacaaacca
      421 gggttcttgc gaatctcctc cgccaccgat ccctagtcct tgacgttgac caatcgtata
      481 atacatcaag ccagcatgtt gtcctttgac ttcaccatca agagtgacca tatttccttt
      541 ttttgctggc agatagttgc tcaaaaattg tttgaagttc ttttcaccaa taaagcagat
      601 tcccgttgaa tcttttttct tagcagtcgc aaggcctgcg cgttcagcaa ttgcccgtac
      661 ttctgatttt tccatgccgc ctagagggaa catcgttttt gaaagctgtt cttgtgataa
      721 ttggctaagg aagtaagttt gatctttatt attgtctact ccacgaagca tgtggaccac
      781 gccgttttca tcgcgagtga cttgcgcata atgtccagta gctacataat cggcgccaag
      841 atccattgca taatccaaga atgctttgaa tttgatttct ttgttgcaca tcacgtctgg
      901 gttaggtgta cgtcctgcgc ggtattcggc taggaaatat tcaaatacac gatcccaata
      961 ttctttttca aaattcacag agtaataagg aatcccgatt tgagcagcta ctttcgctac
     1021 atccttataa tcttccgtgg ccgtgcatac accgttttcg tccgtgtcat cccaattttt
     1081 catgaatatt ccgatcacgt cgtatccttg ttcctttaac aaaagtgctg ttacagatga
     1141 gtcgacaccg ccgctcatcc ctacgacaac gcgtgttttg ctgttgtctg tcatgatatc
     1201 accatcattt caattagatt ctgaaacatc tacgagttga aggtcgcaac ttttcagtat
     1261 tttacattat acgtcatatt tctgaaaatt tcatccctta attatcaagt cgtaaaaata
     1321 ctgttctact ggacctattc agtacacaaa catgatagtc aaccaaaaaa tcactcatct
     1381 tttgagcatt cgatataaaa aattggcata ctcataaaaa gggccagcct ttcggcaata
     1441 aggaataatt ttataaattg tgaaaaacta tttttcaaaa ttgcttctta tcgcttaaag
     1501 ctgcacgtct catttacaac cttttctttt tagatgttat gagttgctca gataagtgat
     1561 tttgtttact ctaagaaaaa agggaggcaa aagatacatg aattacttta tcagtgatat
     1621 gcactttttc catgaacgct tattaggaaa aaacgacttt gctccgcggc tctttgcaaa
     1681 tgtggatgaa atgaaccaac agttgatcac tagctggaat gcagtaataa aagaaaccga
     1741 ccatgtttat catctaggcg accttgctat gcatccgcaa tatgaaaaag caaatgcaga
     1801 aattttggaa ctggtcactc aactgcaagg aacaatccac ttgatcaaag ggaaccatga
     1861 cagtcgcgct tttttccgtt atttagaaaa aaatgatcct aggcttactg acggaagtcc
     1921 caaattttat ttttatgatg taggtgccat cgttaaattc gaccatcaac aatattactt
     1981 gactcattat ccgatgttgc tcgggcctaa cgacactatc cgcaatcttc atggacatat
     2041 ccatcattac agcgtaccta tcgagaacga tgtcaatgta ggcgtagacg caccggaaag
     2101 agaactctta aaggaaaaac taccatttgg cactccgtta tctgagcaac aaatcaaaga
     2161 aatctatgaa gcaaagagga agtacgatag gtcgtgaaaa aactgcccta tatcaagtat
     2221 taaacaaata aagataatta ctcatatttt tcatttgagt aggcttattg ccacaggaac
     2281 attaggatca gaaatggagt tgtaacaaga cttctgtcac aactccattc catactattc
     2341 gtctttttgc tcatggtgtt cttttaccag ttttttctgt aattctttat cataatttcg
     2401 ggcagctgac gttaaaaata aaacgattcc gcataataaa atcccgattc cggaaaacaa
     2461 gaacattttt tctaccccta aacgatcagc taatggtcct gcaaataata atccaattgg
     2521 acctgtcagg ttcatcaaag agttgaagtt ccctaagaca cgacctaaaa cattcgattc
     2581 ataactctgc tggatcattg ccatcagcag tgtattgaaa tatggcgttg cacaacctgc
     2641 tcctgcattc aagataagaa agtacaagaa cccttgacta tcgtttggaa ccaatccgct
     2701 tgcaccgatc gtcaggccca taatgatgta ggctataata ataggtttca ttcggtcttt
     2761 ccatgttcct ataaaactga tcagtgcacc gcctaagagc attccgactg cgtagactac
     2821 ttccactgct cccgcctgtc caacggaacc accaaagtaa tctagcgtca tcaatggata
     2881 cagactgatt gctggcatga acaacagcat cacaaatgca ccattgatcg ttatgtacca
     2941 taacccttta ttatccatca attgctgcag cccaaatttt gcatctttaa aaatagtaag
     3001 cggaacttcc aatatttcag gagaaacttt tgggatcttc acaaaaatca ataagccgac
     3061 accaaaaact gcccccaata catctaataa aataaggtaa ttgacaggaa cgactgaaaa
     3121 caaagccgca cctagagctg gcgcaatgat gaaattcgct gattggacca ttcctaactg
     3181 tccattcgtc cttgttatgt gagaagatgg aacgattgtc ggcataatcg actggatcgt
     3241 tggcatttgg aatgtttgtg cgaccgaacg cataaacaaa gacacaaaaa ctagccaaat
     3301 cgggaaggat tctgaaatcg ttccgactac tgctaaaata agcgcgaaaa tcgctacaat
     3361 aatatccgtg acaatcaaaa gagctttttt atcccagcga tccactagcg gtcctacaaa
     3421 tgggcttagt aaaaccatgg gaatcattcc tagtaaagta gcaaaactca aaattgtcgc
     3481 cgaaccggtt tctcttgtca aataccagat gatcgcatat tggacgacca tactagtgat
     3541 tcctgacaaa aactgtcccg ataaaaagaa caaataattt ctcttccaat tcgttaaaac
     3601 gaccgactgt tcattttcca taaacatccc tcttttcttt ttcattttgt atagcgtttg
     3661 cacaacaatc attatacggg attctttttt caaaaaatac ctttttccct aaataacaat
     3721 cgtttcaatt taaatttctt tacttagaaa caataaatca gcaaaatatt aaaaataaca
     3781 acatgtgtta aatttaaaaa taaaaaagac aactaatttg cgtcgtcctt acaaagttag
     3841 tcatctaaca ataggccgtt tcaaaactag ttgtcttttt atatccacag atcattcgac
     3901 tttttcgaat aacccacggc taatcatccc atccatcaga aaataaaatt tatcttggct
     3961 cattttctgt tttggatctt tgaaaatatt gactgctttc aatccatttg ctgtagtgat
     4021 cgacattttc aatgtttgaa tatcttttga tacagtgatt tttaatttga atccttcttt
     4081 gctttgcggc gtggcatcta atggtcggat caattgataa ttgcctccgt ttgtttctgt
     4141 aaatccatta tcgcgcaatg tatatctttt tgcatcagga tgcaaacgat agctaattgg
     4201 tgcgcccagt accgttgctg ttgtttcaaa tgccatgatt ttcacctcga taaaattagt
     4261 ttctctttat tataaaagtt tatcctgatt tttactaggt ttttcagtta agttccctaa
     4321 aaaatgaagg cgttctctca gacatctgtt catataagaa tgacttcact agttattttt
     4381 tgacagcacg aactaattga tcaaggaaat actggatgtc gtcttcggta tttcccaaac
     4441 caaagctgat acggatggat tcttttagta tcggtgtatc ttctccatac attgcttcca
     4501 atacgtgtga aggctcgata tttcctgctg tacaagctga accagtagaa atagcgaccc
     4561 cattaagatc aagcttcatc agtagcaaat catttgcttc gccaattaag cgaagattca
     4621 acacatgagg gagtttattc gttaaatcac catttacttg ataagggatt tcgtttgctt
     4681 ctaatgtatc aagaatcaat tgctggaatc ctttatattt gttgttacgt gcttcacgtt
     4741 cttcaggtga cagcagttca actgcttttg ccatacccca gatccctgct aaattttctg
     4801 tacctgcacg ccgcttttct tcctgctccc cgcctaaaag caatgcaggg atatttactc
     4861 cgtctcgttt atataagaaa ccgacgcctt ttggcccatt gattttatga gcagagatac
     4921 ttaataaatc gattcctaat tcttctggat ggatcgtttg gtttccgtac gcttgtaccg
     4981 catctgtatg gaaataagct ggatgatccg ctaaaagttc accgatttct ttgattggca
     5041 tcaaatttcc gatttcattg ttcccgtaca tcacagagac caagatcgta tcttctcgta
     5101 acgctttttt gacctcttcg atcgacagat tccctttttc atctacaggc agatatgtaa
     5161 cgtcaaaccc ctttgtctct aaatatttca tggtattaag aacagcagga tgttcgatag
     5221 ctgtagtgat tagatgtctg ccttcttttt gacgcgagaa cgctgtttct aagatagctg
     5281 tattatcccc ttctgttccc ccactgttaa agatgatctc gtgaggtttt gcttgcagac
     5341 tgtctgcaat gatttgtctt gctgtttcca atttttcatg cgcgtggcga ccaaatccat
     5401 gtatgcttga aggatttcca aacgtgtctt gcatgattgc cgtcatcgct tcaattactt
     5461 gtggatgcat gggggtagtt gccgcatgat ccagataaat agttttcact tcattcatcc
     5521 cttccaaatt attgctaggc acattctagc ataaaatagc gaacaaacca aagaagagct
     5581 catttttgtc tagaggacgt ttcatgggca tagaaaaaag gagctgtgac aaaagtcttg
     5641 tcgcagctcc ttgaagctgc tcacttctgt cacaacttct t
//
LOCUS       DACDKL010000150         5636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDKL010000150 DACDKL010000000
VERSION     DACDKL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.152"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..577)
                     /locus_tag="IV329_002183"
     CDS             complement(14..577)
                     /locus_tag="IV329_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8941348.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(592..1635)
                     /locus_tag="IV329_002184"
     CDS             complement(592..1635)
                     /locus_tag="IV329_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941349.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1768..2172)
                     /locus_tag="IV329_002185"
     CDS             complement(1768..2172)
                     /locus_tag="IV329_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8941350.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2405..3040)
                     /locus_tag="IV329_002186"
     CDS             complement(2405..3040)
                     /locus_tag="IV329_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8941351.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3306..4358)
                     /locus_tag="IV329_002187"
     CDS             complement(3306..4358)
                     /locus_tag="IV329_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8941352.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4371..5309)
                     /locus_tag="IV329_002188"
     CDS             complement(4371..5309)
                     /locus_tag="IV329_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8941353.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1765 a         1198 c          917 g         1756 t
ORIGIN      
        1 aggtccgtta tatttactca aaattgattc tacgaataga tcttcttatc atttctattt
       61 tagtagtcat gcgttgttgt tcttctaaag gtggtaatgg tataagtagt ttagataaat
      121 ttgtaccatt tacatttggt tgcccagttc cgtaagaaat tttttctaat aacttccaat
      181 ataatggcga gtctaagaag cagtctacgt attctgataa taatttctca actaacctaa
      241 ttcgaatcaa gtaagaagca aaaacagatt cctcacttat ttccttaacc aagaaacttt
      301 ttcccatagt tcctcctgtc cttgctatca aaatgtcatt ttcttccaat cttaaatcaa
      361 ctagtttaga attactaata tcacagtaag gcacactgga ccaatttaca cgtccctctt
      421 gtatatctgt gattctcaaa aacttaacat tgccttgttt tttcgccgaa tctgtatagc
      481 catattgaat cttagtagaa atacttccta aataaaccca tagtcgaaca tcgctagatg
      541 atttttcttg tcgttcttta tgaacaacag tgatcatctt gagttgtccg actatttttt
      601 agttgcagca atctttttgg gacgcttatt tgcatgaagc aacgttgcta agtctagtaa
      661 taaaacattg gctaacacag gattggcttt ctcgcgatac gctgcggcgc cactttcaaa
      721 caatactgct agacgatctt ctacaacaac gatttgttcc aaatacggtg gaaatttgat
      781 tcgttttagg atccggctag gagagtcccg ctcggacact aagagttccg aggtataagg
      841 accataagaa cgactggcta agagataatg gccataaaaa gccacacctt gaatcttatc
      901 agatgttttt ctgctcattg tcacggttgg ttggattttg tctttgcggc gaattgctgt
      961 tttcatttca gctatcgtcg gcaatagata gaaagtcaac tgtccttcac cttttagtgt
     1021 gaagttgcca gataccagtt gatcattgtt ttgagtcaaa tacgatgctt gtggaatctc
     1081 tgagagatca gttgtcagcc aataagcgat tggctgtccc gtctcttcag atgtatctgc
     1141 taagatatcg atcaaacgta aagcggctac tcgtccagta gcagatcctg tgattgtcaa
     1201 ccataaaagt tcccgttttg tatcatatac taagccccct acatgtggcc gtttaggtaa
     1261 aatcagcagt ttgatcggct gtcctgtttc tctatccagt acatgaataa tagaatgatg
     1321 ctgatggtca tagcagtagg cagaaataaa cacaaaatct tgagaaaaag ccaacccttg
     1381 cggcgtcatc cattgacaaa cctcattttc gccggtttcc ggcaccacca ttcttgtttc
     1441 tgttaatcca ggaatgttat attgttttgc ttcagccttt tcccaagctg cttgaacaat
     1501 aggataccgt tttaactttt tttcaatatc ctgatgtatc cgttgctgct tccaatgctt
     1561 aaactttagg acatagtaaa caagaaggcc aatgaccagc aacactaaaa tcattattgc
     1621 tacccgctgt gtcatttttt tgcctccttg ttctattcga tcaatcgtcc atataccgct
     1681 attaattacc gtaactacat gtttctttta ctagcgtttc tttataggat ctttcataat
     1741 ctcatttata gtaacattaa gaatccctta ttctactata gtcacttcca catcaatatt
     1801 gcctcttgtt gctttagagt atgggcaaaa tgcgtgggct tcttctgcga ttttctctgc
     1861 ttggtccttc tcaagtcctg gcaaagaaac ttctaaaacg accccgattt ggtagccccc
     1921 atcttctaat tgaaataaag atacccgtgc tttgacttca gaatcagact caagattatt
     1981 ttcttccagc atgtgttgaa ccgcgccgtt gaagcaagat gcgtatccag ccgcaaaaag
     2041 ttgttccgga tttgtccctt cactatggat tcctgggcgg tcgatcttca tgtgcatact
     2101 tccgtttggt gcctcaactt cgccatcacg gcctccacga ttgatcattg ctgtttcgta
     2161 catttttttc attgagatca tcctttcttt cgtcgttcta tattaagtgt aacgagtatt
     2221 ttttaatctg acaaaggtaa tgcttgcatt cggcgacatc agttagacaa catcgatgat
     2281 cagtgataga agcagtttat ctatacaaaa aaagaagggt tctatgatat ttggtgtcct
     2341 tactcgcctt gagtaacaac accaaatatc atagaaccac ttaatcagct tcacactgct
     2401 ttttctactt tcgtagttga aaaaaactta aacaaatcat cggcaaatag ttaaagaaaa
     2461 aacttaatcg ttgaaagaag cccaattgct taaagaaggc aaactgacta atcgacggaa
     2521 tccgtgctaa tccgtaaaga cctgcaatta gtaaactaat aactgttaaa acaagataaa
     2581 aatggctaag cgtaccttga ccactttgac gataaagcag aacaagtaac aatgggaaaa
     2641 gtagaaagcc tgcatagcct agcccagaac ctatattatg gatccaagtc gatagattac
     2701 aagagctttc attcgtattg atactgaata agccagtaaa aatacaatca cctatcccat
     2761 ataaaccaac tgttgcaaca acaagtattg caagtgtttt tgatgttcca acaaataggt
     2821 gatacaacgc aggcaacgaa agaacaaaaa aaagtccaga tacaactgac cagaccaaga
     2881 aagcacgtct gactggacta tctacgtcac caaacacact aattacggta gtcatttgat
     2941 tcagtttggg ataaaaaagg ccaaggatat acggtgtcga taaatcactc atcacaccta
     3001 ataataaaaa ataaaaacca tatttttgca aaaaaatcat tttcttcccc tactttttag
     3061 tgattattcg atacatccaa caatcaaatt ttttctcatt ttaacacata tttttattct
     3121 agttgtcttt tgaacaatca ataacataaa ggtgagtttc ttctacattt taaaaaatcg
     3181 aatggcactg aagagttcag ttgtcagcat caatctccta ctcaaaaagg caaaaaaacg
     3241 taagtcagat gttgttgaca tctcacttac gcatccattg attgaaaatt tcttagtaaa
     3301 atctactagc ctaaaaattt gaagacttct tctcgatcat acagtttaaa tactttctcg
     3361 cataaaaatt gagtaattag tgccgcaaaa acagggatga ctaaccaaca aagaacaaca
     3421 agaaagccat ttagccctgc atctaaagaa gccaacggac caacaagtcc gaccacgccg
     3481 aaaccagcag aagcaggcgt acctgaaata gaaaatagag cgacaggaat agctgtaatc
     3541 actgcactaa ctaaacaagg aataagaatc acaggttttc taaatagatt aggcatcatc
     3601 atcttcatag cgcctaaagc aatcgcaatt gtcacacctg acttattaac cttccaagag
     3661 ttcactacta aaacaaaagt ggttgcagct acgcccatcg cagccgcacc agcagaaaca
     3721 ccatctagtt ggattgccat tccgattgct actgttgaaa taggagagat aatcaaaaag
     3781 gcaaaggaac aaccaatcaa gatagacatt aacagcggtt gtaaggtagt gaagttatta
     3841 atcaatgatc caattgcaga agtaattgct gttacaaaag ggaataaata aaaaccaagt
     3901 aaacctgcac ctacacctac aacaatcggt gttaacacaa tcgacactga cccaaaacta
     3961 tttccaatca gtaaaatcag cccgacagca atcgatgctg tcagcatcgt attaataata
     4021 tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca aatcaggtgt gaatttgact
     4081 acaccagaac caacaaaaga agctccggct acaaccatca ttctcatcgg atcaaatcca
     4141 aattgtaagg caatcaaacc accaatgatc aaaggtgttg ctagttgaaa aataactgct
     4201 aactgtgcga tcgttaccgc aaagggatac gcactaaaat attttaaaat ggcacctaat
     4261 actgcattag gaatcaatcc aataatgatt cctgttgctg taccagcgag gactttattc
     4321 agaaaaattt tcggtgatat tttttgtgaa gtttccattc aatctctcct ttattttacg
     4381 ttcaaactat cttcttttgc atggacaagc tcagtcaaga aatcacaata aggcgtcgca
     4441 acaccatatt tcttcccttt tctagaaatt gcgccattga tataatcgat ttctgttaat
     4501 cgatggttct taatcaagtc ttgatacatt gaaggataat gtaacccaat tgtttccggg
     4561 tcaaaacaag attcacaatg tgcaatgact tctgggacat ctagttcaat cttctctact
     4621 gctgctactt tggcaaactc attgacaatc gtcgccacca ttttatgagc agtcgatgtt
     4681 tttcctagct cagccatatt aacgtctaag attgtgcata gtccgttcat tgttccgttc
     4741 acacatgctt tgcgataaat agaatagtga atattgtcag aaaaatgagc atttaatcct
     4801 gattcagaca atttgtctgc taattttttt gcagctgctt cttttccatc acctaaattt
     4861 tgtaattcta ctgaaccact tccaaataat ttgacctgac caggaccttc aagacctgcc
     4921 gtccacatgg tatttccaat atagatattt tccattggta caaatttctc aataatatct
     4981 tcatgcccga taccatttaa tagacataag acctctgtat cttttttgat taatgattgg
     5041 atatcctgca gcattttttc cagctgcatc gctttggtaa atagaataat cagatcaact
     5101 tgatcttctt tttctacttc ggattgaaga acgattggta gttttgcttc tacctcttta
     5161 ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt ggacatgttc tgcccagcca
     5221 tcgattaata atacttcgtt cccactttgg tgaagcatca acccaaagcg acttcccatt
     5281 gcgccagcgc ctgcaattgc tattttcatt gattcttcct cctttttctt tctattatac
     5341 tcatttgaga atcttttcac aacctcgttt tgttattctt ctcttattta tcatgtttat
     5401 atccaccatt caagttattt tatcagcaaa tcaataaaaa ctggaataga atcccaattt
     5461 catcgtaaga gaaactagac tagcaataag aaaatagaaa cattcataga aaaaatctca
     5521 ctcctctgat tataaaagag ggggcattaa cctcgtgtat actaaaacct tccattcatt
     5581 cgatggatga ttttcttttt gtgaaaaaat attcattgta gtacgatgtt gcgcaa
//
LOCUS       DACDKL010000151         5625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDKL010000151 DACDKL010000000
VERSION     DACDKL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.153"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..420)
                     /locus_tag="IV329_002189"
     CDS             complement(61..420)
                     /locus_tag="IV329_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941354.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(392..763)
                     /locus_tag="IV329_002190"
     CDS             complement(392..763)
                     /locus_tag="IV329_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8941355.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(780..1082)
                     /locus_tag="IV329_002191"
     CDS             complement(780..1082)
                     /locus_tag="IV329_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941356.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(1099..1569)
                     /locus_tag="IV329_002192"
     CDS             complement(1099..1569)
                     /locus_tag="IV329_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8941357.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(1544..1822)
                     /locus_tag="IV329_002193"
     CDS             complement(1544..1822)
                     /locus_tag="IV329_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8941358.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(1819..2868)
                     /locus_tag="IV329_002194"
     CDS             complement(1819..2868)
                     /locus_tag="IV329_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8941359.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(2825..3754)
                     /gene="cpaF"
                     /locus_tag="IV329_002195"
     CDS             complement(2825..3754)
                     /gene="cpaF"
                     /locus_tag="IV329_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8941360.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            3886..>5625
                     /gene="ade"
                     /locus_tag="IV329_002196"
     CDS             3886..>5625
                     /gene="ade"
                     /locus_tag="IV329_002196"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8941361.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDE"
BASE COUNT         1763 a         1151 c          990 g         1721 t
ORIGIN      
        1 acattttttt attaaaaaca gtgttataac agaaaagagg gcgttttcac gaacattcca
       61 tcattctgta ctagtatctg tatcattttt tacttcttct gtgaaccgaa agataaacgg
      121 tgagacttcc acctctaaga atagggactg ttgtacccac tgatatgtta cagatccagt
      181 tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta gactgttccg ataaagccat
      241 ctgcttcatt atttttgcta tataataatt tttgattcgt atattaagat ccattgacaa
      301 acggtagctt tctaatgctg ccatcaataa aaacgtacat aaaagaaata aaagcaagac
      361 agaaaataaa atagagccct tataacattt tctactgatc gttttttgtc catatggcat
      421 atccatcttc tccattttca aagtgtacat aaaaagaaat tgtgtgttct ttttccaata
      481 attgaagctc agtgatactt gttaacatag gttgatgtcc gccggaagtt cttcttttac
      541 gtatcattgc ctgatatctt tcgatgatat actgtttatc ctcgatcgtg tagtgcagtt
      601 gctgattttt cacagagaca ttgtgactgc tcttgagttc ttcttctaat tgtgctaaaa
      661 atattagcca ctctttttga tctttacgct gcaaaaattc acttacttgg atggtttgct
      721 gaatcaaaag attgaatgtg agcaatagac tgctcaatat catcagtcca attagacatt
      781 caatcagcgt aaatgctgga attacttgtt ttttcaatac tgatcgcttc tttctccata
      841 ttgacaaaca ctttttcgat tactccatta ttatcgacaa tttggatatc gtatgggatc
      901 atttcttccc ttcgctctgg tcttttgtga tacatccgct gcgtcttgat atcttcataa
      961 atcattcgaa taaatagcag ttcctcccca cttttctttt ctgcctgaag caaaaacagc
     1021 tggccaaagc tcactaagta gacacagaca gaagcgatca taagcgctgt aagggactcc
     1081 aacaaaacaa atcctgtttt attcaattct ttttgtatag cgaccactcc ctatctggaa
     1141 ttgatacgta atcgtccgct ctttttcttc ccaataaaat tgataagctt ttagtgaact
     1201 ttcattcccg gttctagcag caaaagtaat agaagcatgg cgtttcaagg taatctcatc
     1261 aggaatatct aatacttccc aatttatttt atctggattc ggaacatgaa aaatgatttg
     1321 attctcttca ctattccaat aaaagcctgt ttggatctga ttcacaatag cgattttttg
     1381 cgtagaatag attcgttttt caaattggcc gaaaaattga tagactgcca attcattctt
     1441 ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt gtaagtaata aaatcagtaa
     1501 gagtgattca aacaacgtat atcctgatgc taaaacctta ccttcaaggc cgctgataga
     1561 tttctgcatg ttctcgtttg atatagcctt cattcaacaa ttcctccaca gtaggaactc
     1621 ttccattatt ttgaagcgca tagagctctt tttgtgtgtc taccaactgg atgatcgctt
     1681 cacgactttc ttggtctaca tgattcctat aacgcgacaa attagggacg aacaacaaca
     1741 ctaatacact gatgatcagt aagacgatta gcatttcgat caatgtaaaa ccgctataat
     1801 aagatatttt tctctttttc atggcaaaac ctcctctatg cttccgtaaa caggtaataa
     1861 aacagcggca taaactaata aaatcaaagc cgctacgccc aaaaaaatga ttggctgaat
     1921 aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt cttgtccatt cgctgtaaaa
     1981 aatcagttct ttacctaaac ttccctttgc ctctccctgt aaaacaattt tactgaattc
     2041 ttctgataga aaacgataat tctggaactg ctcagccaat gacatacctg cctccaatcc
     2101 ttgaatcaac ttagaggcaa gatgctgaat cagactttct tgtttcgttt cctttaagca
     2161 gactatgatt tgccttaatt cgaaaccttc atgaaaaagc tttccaatct ctagtgacaa
     2221 ataagccgat tgatacaagg aatatatcgg tccaatcaat aattgtctgc ttatccattc
     2281 actctgatca acaggagaca acttcatcag acgatacctt actagtaaag ccaatagagt
     2341 aaccgctagg atgaatagta gaacatacca atgaaagact tgtatcagct gaatacccca
     2401 atgctcttgc atgaccattc ctgaaccaat aagctgaggc aaaacgaaca accgcatact
     2461 aaacaagata cctagcaaaa aggtgagcaa tagcatcgga tatgcaagca gctttttcaa
     2521 atcattgcga aatccttcaa caagccgaaa ctgtttggca atttccaaca aagttgtcgg
     2581 cagattccca tgacgttcag ctaactcgat ctgtaccagt tgctctgctc catatcctat
     2641 ttgcgaaagt acctcactaa acgcttgccc ttcctttagc tgatgttgga tatttgccaa
     2701 aataacaggt ggaaacgaac ggatcgtgag taataaataa agggattctt gcagactaaa
     2761 accagataaa agcagttctc ccatggtttg tgcaaactgg ttttgctggc ttcttgttaa
     2821 tcctttagaa tagttctgac tgccaaatat tttcctgagc ttcttcataa ctttcctccc
     2881 atgatttttt tactgattct tcctccataa attgataact ccataaaaca gaaggatggt
     2941 gattggcatc ttccaaaagt tcttgataaa tgacaccagc taaacattca gatagctctt
     3001 cttttttctt tgtcagttca tggattctga ccaatgtacc ttcaagattg cgtgcatgta
     3061 cggttgcaaa aacccggtgc cctgttagtg ctgctcttac cgcagcatgt gcagttgatt
     3121 catctcttat ctcgccaatg attagcaagt ctggacgatg ccgtaatgcg agtttcaaaa
     3181 gtgtatcata agtctgtgct attttcggat tgatctgtag ctgcagaaaa aacggttctt
     3241 ctatctctac aggatcttcg atagtaatca cttgcaattt ttcttccaaa gccaaccgat
     3301 acattagaga agtcttgcct gatccgactg tcccgctgaa aaggtataat ccacgtctga
     3361 cggttttctg taaaatagtt gcaaaatctc gagaactaaa gcaacgatgg ggtgtttcaa
     3421 aagtaccgta taatagccga atcactaaac tctctcgttg aaggtaatct cctacagaag
     3481 acaaccgcag acgaacttcc tgtttttcta aagaataagt aaatgcacct aactgagctt
     3541 ttcttttttc tccgatatcc atctgtccca aatacttaaa tcttgagatc agttgctgtc
     3601 catctgccga agagactttc ttccagaatg ttcgtttttt tccatagcga aaatagatgt
     3661 aaaatacttc tgaagctggc aatatatata cgtcttgggc atgtttggca aaactttcac
     3721 ttatcatttg atccgataat cgttttattt ccattattct cccctccaca aaaaacatac
     3781 gtctttttga tctttttgtt tcaaataaaa tgaatttaca aaaaaatatt ggcaaaagcc
     3841 gaatttatgt tatacttata aaaatttatc ttgggaggca ctccattgga aacaacatta
     3901 aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg acctagtgat caagcatgct
     3961 aagatcgtca acgtctttac gaaagaaatc atggaaaaag atgtagcaat ctgcgaagga
     4021 atgatcgtag gaatcggtga atacgaagga aaaagcgtac ataatgctaa tgggcaatat
     4081 ctagtgccag gattcatcga tggccatgtg catatcgaaa gctcactttt atcaccaagc
     4141 gaattttcaa aagtatcctt gcttcatgga gttactactg tagtgacaga tccccatgaa
     4201 ataggaaaca ttgctgggag tacaggtctt gagtttatga tagaagatgc aagacaaaca
     4261 ctcatgaata tcttttttat gctaccttct tgtgtacctg taaccccttt tgagaccaat
     4321 ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac aaaaacccga agttttaggc
     4381 ttagctgaag tcatgaacta tcaagcagta gcaaataatg aaacagacat cctagaaaag
     4441 atccaattga tgcaccagca taagaaaaaa attgacgggc atgcagctgg tatcggcatg
     4501 gaagagttaa atgtttatcc tgcagcaggt attcggacgg atcatgaagc tacaactgca
     4561 aaagaagcta aagaacgttt ggatcttggt atgtatttaa tggttcgaga agggaccgtt
     4621 gcaaaagatc tggcatcgct actaccggcg atcacacctg aaaattcaag aagatgcttc
     4681 tttgtcaccg atgataaact gatcaatgat ttagtaaaag aaggatcaat cgatcatatc
     4741 gtccgtcaag ctattgcact tggaatcgat ccattacaag ccattcagat ggcaacattg
     4801 aatgcagcag aatgttttga tttgaaaaca ctaggtgcga ttgcccctgg gtatcaagct
     4861 gatttcttct taacagacga tcttaccaag ctgccaattc atgaagtttt ctataaggga
     4921 acattagttg ttcaatctgg gcaactccaa gaccatttat ttgctgatca ttcaaattca
     4981 tatacgtgtt gtctgccaaa actaaacgct caaccattaa atccacattc gttagagctg
     5041 cttctttctt cttcacaagc ccatgtgatc gaaattatcc cgaatagtct agtgactaaa
     5101 gatttggtag aaaaagtgga ccgacaaggc gaacgattca ttccttcaat cgaaaaagac
     5161 caattaaaga ttgcggtgat cgagcgtcac catcaaacgg gaaacattgg attaggcata
     5221 gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa cgattgccca tgactcgcat
     5281 aatctagttg tcgttgggac aaacgacgag gatatgctgt atgctgctaa tcttttgatt
     5341 caaaaaggtg gcgggatgac cgttgtcaac aaacagcagg aaatagcttg tgtttctctt
     5401 cctataggcg gaatcatgac acaggaacct ttttcgatcg tctatgaaca gttgaatgaa
     5461 ttaataacta aagcacatca actaggagca gctaaaacct ttgatccttt tttgacactt
     5521 tctttcttaa cattatctgt catacccgaa ttgaagatca ccgacaaagg attattttca
     5581 ttttctcgct tccaattgat tccaccttgt ggaaaaaccg atgaa
//
LOCUS       DACDKL010000152         5538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDKL010000152 DACDKL010000000
VERSION     DACDKL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.154"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..641
                     /locus_tag="IV329_002197"
     CDS             <1..641
                     /locus_tag="IV329_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941362.1"
                     /translation="FLLIACLTLYHLQSALANQVQDRLSFIGASASTELLPALELASR
                     FYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWRLTGLSKTKLFLFIFWQLFFPLF
                     LCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDILTSVSGLTIPMDQQTFFT
                     IDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNET"
     gene            631..1140
                     /locus_tag="IV329_002198"
     CDS             631..1140
                     /locus_tag="IV329_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941363.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1210..2658)
                     /locus_tag="IV329_002199"
     CDS             complement(1210..2658)
                     /locus_tag="IV329_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8941364.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2658..3374)
                     /locus_tag="IV329_002200"
     CDS             complement(2658..3374)
                     /locus_tag="IV329_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8941365.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3355..3705)
                     /locus_tag="IV329_002201"
     CDS             complement(3355..3705)
                     /locus_tag="IV329_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8941366.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3849..4622
                     /locus_tag="IV329_002202"
     CDS             3849..4622
                     /locus_tag="IV329_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8941367.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4793..5159
                     /gene="ssrA"
                     /locus_tag="IV329_002203"
     tmRNA           4793..5159
                     /gene="ssrA"
                     /locus_tag="IV329_002203"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1617 a         1090 c          966 g         1865 t
ORIGIN      
        1 cttttttatt gatagcatgc ctgactcttt accatttgca atcggcttta gccaatcaag
       61 tccaagatcg gctttcattt attggcgctt ctgcatctac agagcttctt ccggctttag
      121 aattagctag ccgattttat ataaaaggca tattcagttt attttttcta tttgccttcc
      181 tttttatctt tttcttttac tattcagtca ggcaaaacaa gcaggaattg atcaattggc
      241 gcctgactgg tctttctaaa accaaattat tcctttttat tttctggcag ctgttctttc
      301 ctctctttct ttgttgtatc ttggttttat tgtggatcgt aagcttccag tcagtttatg
      361 aagcgcttct tcaaaggatc aattttcttg tcttaaagac tactgaactg ccagatatcc
      421 gtgatatctt gacttcggta agcggactta caattccgat ggatcaacag acttttttta
      481 ccatcgattt ccaaaatgat ctttttttca cagatatact taagggattt gcccaaactc
      541 ttattgggct cagtggatgc tgtctcattc ttagtctatt ccctttcact ttttttatcc
      601 atcatttgca aagtaggagg cctggacgta atgaaacctg aatatttgat tcatgctgaa
      661 cacttgaccg ttattttgtg tcctctcgaa aaacagcata aaatattgga gcacttgaaa
      721 aaagaggttc ttgttgcaaa actggataat tttgcgatca tccaaaaaaa ttggccgatg
      781 tttccttatc tgaatcttaa agaccatgtc ctgttggatg taccggaaaa agaaatcaaa
      841 caagatcgtt tggcctatca agaaaaactt gatatctctc cttccttgct taattgtgcg
      901 gcagctgagt tgacgtcttt tgaaaaagta aagctgcaat tacttcatgc cttgctttcc
      961 aagcgaaaca atattgttat tgaagataca tttgatgaac ttagtgtatt ggagatccaa
     1021 gagctactcc acttgctttc ttatttagct catgaagaaa atcaaggtat cttactcttt
     1081 actcatgatg ctactattgc tcaaagtcct tatattgatc gtttagagcc cgcgggctaa
     1141 atcaatcatt ttatagtaaa aacaagcggt atagaagatc gattcatgat cttttatacc
     1201 gcttgttttt tactcttttt tgatttctac gtcaggcaaa cgaacagcga attttgttcc
     1261 ttttcctagt tcgctttcta cttctaattt tcctttatgc aagcgtacaa gttgctgcac
     1321 gattggtaaa cctaatcctg attcaccata ttttgtattt tttcttgaag gatctacttt
     1381 ataataacga tcccagatac ttttcatttg ttgttcagac attccaattc ccgtatcttc
     1441 aatcgtgatg atcgtttcaa gatagccttt ttctaaagta atcgtgattt ggccgttttc
     1501 agtaaactgt atcgcgtttt gaataatatt gaccatgatt tggacaaatc ggtcataatc
     1561 agcgtaaaca tcaatatcat caacggcttt caatgtcaat gtatctcctg ctgcttctgc
     1621 tttagcttct aactgtgcaa caatattttt caatgtttct gttccattga attttttaat
     1681 gaccatactg atttgattcg tacggatttt ttcataatcc aagttttcat tcaccaaacg
     1741 gatcagacgc tctgtttcat ttttcatcaa tttaatcgcg ttttctcgct gattttcagg
     1801 gattgcattg tattctaacc cttccagaag tccgttgatt gtcgtaagag gtgttctcat
     1861 ctcgtgagaa gcatctgcca taaattgtct tcgacgattt tcttgttctt caatttctgc
     1921 ttgagattct ttcaatgaat tcgtcatttt attgaaatca tctgctaact catcaaattc
     1981 atctttgtca tgtacgggga gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc
     2041 attccttaac cgattgatac gcttaacttg gaatgctgca aaagcatagc tggcaataac
     2101 tgcaacgata ctggaaaaaa taaatccttt gaacaggttc agtgtaattg gacgaacgct
     2161 gttatcgata tttcgcgcag gttgactaac tacaagtgct ccatagaatt catgattcaa
     2221 attaaatggc acaagtgcgt aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga
     2281 tgtgaatttt tcttgacgtc cattcctcag atttttccat tgatcagctg ttaaggtaaa
     2341 attcagtaaa gctgtattag tcggataaat gacacgttcg tttttatcga taaatataaa
     2401 gttcacattt tgttctgtca gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg
     2461 atcttgacta aactgcggga gcgtatcggc ataggtttgc gttgtctttt caactgattc
     2521 agcatatcca aacagttgct ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt
     2581 aaaagaaact ccaacgatca ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg
     2641 ctgatacaaa taacgcatta ggcaacacca gaatcatcga atttgtagcc gactccccaa
     2701 acagtttgga tgacttgtgg tccgaccttt tcgatttttt gtcgtaattt cttgatatgg
     2761 gcgtccactg tccgttcatc accaaagtac tgataatccc agactaattc aagcaactgc
     2821 tcccgagaga atacttgacg tggttttttt gctaaagtaa acaatagatc gaactcttta
     2881 ggcgtcagtc cttcaattgg ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc
     2941 ttgaaatgac tagtctcgat atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct
     3001 tctgccaact cactacggcg atgcaaagct ttcattcgag caataagcgt cagaggactg
     3061 aaaggttttg tgacataatc atctgccccc atctctaagc cgatcacctg atcgctttca
     3121 gaatctctag ctgtcagcat gataatcggc acagtacttg aaactttgcg gatctctctt
     3181 cctactagca taccgtccag tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt
     3241 tgtgggtttt ctaaaaaggc atcgagccct tcttttccat catacttgaa agttgcttcc
     3301 caaccttcat ttaagaaaaa catctgcatc atttcagaaa cagattgatt atcttcaatc
     3361 attaaaatat tcattatcgt tccctccagt ttccaggtca tatttctgta atttcttttc
     3421 tgtcataata aaaataggca taattggaaa tactagcaga agcatttcaa tcagaaaata
     3481 attcgtatgc ccagatgtct ttaaataata gccgatcaat gccatcagta agcccataag
     3541 aaaaaaccag tttccgcttg tcttttgagc cagccgccaa tgagcgtctg tttttgcggc
     3601 aagaggcgaa tgatacccat aaaaaggcag atttcctttt gagggcagaa tccgaaaaag
     3661 actgccaatt attaagaata agataccaat actgaaaaaa atcattcttt ccctcctaaa
     3721 ttctcttctt atagtatact ctaaattgtt gactgaacca atatgttcag gaattcttca
     3781 cgaaaggttt gtcttttgtt cattctttgt taagataaac ttattccaat aaaaaaggag
     3841 ttttatttgt gtcaaattat cgtgcaatca ctttctttga tcttgatgga acattactag
     3901 atgcgaaatc aaaaatcaca cccgaagtgg caaaagctat gaacgcatta aaagaaaaca
     3961 atgttctccc cgtcatcgct acaggacgta cagaagcaga aatccactct atcatggaag
     4021 atgcgggcat tacttctgcg gtcaccatga atggttcttt cattcgagta gacggcgaag
     4081 aaatctattc tcaaacattt tcttcagaag aatgtcaaaa aatgtatgat catgtacagt
     4141 tgaacggtca tgaactttct tactataacg atcagaaaat ctggtgtacc ggtcatagcc
     4201 aaacaatgat caatgcttac gacttcattc attcagatgt tccggaaatt gatcctttag
     4261 gctttaaaga aaacacagta aatatgttgc tgatcttatc tcaatcaggt gacgaatact
     4321 atcacgaaca ttttcctgag ctaacttttt atcggaatgg tccttactcg atcgatacag
     4381 tgaagaaagg aatctcaaaa ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg
     4441 aaaaagttcc tacttttgga tttggcgatg gtccgaatga ttttgccttg ttggaagctt
     4501 gcgacaacaa aatcgctatg ggcaacgcct atgacggatt aaaagaactt tctaccttta
     4561 tcacaaagaa aaatacagaa ggcggaattg tccacgcatt gaagtatttt gatctgattt
     4621 aagatctttt tttgaagcta cctcttttct taagaaaatc gcttattttt tcttaagaaa
     4681 ggggcagtta ttatataaat aatgggatat gattcgcttt tttattgaat ttatcccaat
     4741 atatgctata ctaattttgt atgaaaaata cacgtaaccc ttgttagcta acggggacgt
     4801 tacggattcg acaggcacag tcgagcttga attgcgtttc gtaggttacg tctacgtaaa
     4861 aacgttacag ttaaatataa ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta
     4921 aaaacagtta gcgtagatcc tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt
     4981 tagtgggata cgtttcaact ttccgtctgt aagttgaaaa agagaacatc agactagcga
     5041 tacagaatgc ctgtcactcg gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa
     5101 cgtagatttt taagtggcga tgtgtttgga cgcgggttcg actcccgccg tctccatata
     5161 tgtatggttg agacagataa acatgtatga aaaacgttgt taaatcaacg tttttttatt
     5221 ttggtcaagg agagaaaagt agagaaatac aaaatttttt gcattgattt tgcattcaaa
     5281 aaaacattca aacatttaga aatccccaat acgtattcag gaaaatctat tttaaggttt
     5341 acaaaaagaa cttttgttcg tacactcttt ttgaggtgaa cttttatgct gatggaagga
     5401 aaaacacaat tatggtttaa gcatgttcct tcaaataagt tttttaatta taaactctag
     5461 agaaattaat tcatcctaac ataaaataat tgttcactca aaagtttttc tagaacaatt
     5521 aaaacagcag gcttcaca
//
LOCUS       DACDKL010000153         5497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDKL010000153 DACDKL010000000
VERSION     DACDKL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.155"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            308..1240
                     /locus_tag="IV329_002204"
     CDS             308..1240
                     /locus_tag="IV329_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941368.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1461..3224
                     /locus_tag="IV329_002205"
     CDS             1461..3224
                     /locus_tag="IV329_002205"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8941369.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3308..3766
                     /locus_tag="IV329_002206"
     CDS             3308..3766
                     /locus_tag="IV329_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8941370.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3875..4558
                     /locus_tag="IV329_002207"
     CDS             3875..4558
                     /locus_tag="IV329_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8941371.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4705..5319
                     /locus_tag="IV329_002208"
     CDS             4705..5319
                     /locus_tag="IV329_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8941372.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
BASE COUNT         1945 a          861 c         1124 g         1567 t
ORIGIN      
        1 tattgctctt tctgagctca cacagtttct gtacggtcaa tcttattttt taaagttttt
       61 aagttattat tttaatggta tattcacgag tacagaactg gctgatcttg aatttatttc
      121 tttgagcaaa gtatatacaa tcaaaaaaat cgtattggat ttttttaaag aaaatgatta
      181 tttgaaaaat aaagaaatca ttatcccaga atttgactcg cggaacatct tgctagcttt
      241 agtacgttat gtcaattggg aaggctatga aaataaaaat ccgtatgtag aagaagcttg
      301 taacgagttg attgattatg tagagactca tttttttaaa agaagatatt cggaagatga
      361 aaagtccttt atcatacgag gaatagagat cgcaatcgga agaaaggcat ttccccttgg
      421 actgaaagaa gaagataaga aaagcgcaga aacgaagccg ttatttcaat tagtctgtca
      481 aggattggaa gagcaaaaag gaattctaga cttacaagaa gaagatgtct attatatttt
      541 ttctttgttc aacagtagaa attataccaa tgagaacatg gagctgttaa gaaaagattt
      601 cgaagttgtt tataaaaatt ttgttctcaa tgacctatca ctgaatgaat tgattttaca
      661 gattattagt atcgctgaaa tcgacgatgc agaaaatttt attttcaagc aatccttttt
      721 acaatttgtc cgtacgttgt gggcagatgg acaagtattt ttaccggaaa ggatctatct
      781 tctatcagaa aaagaacaac ttctgtacgg aaaaattgta gaaatattgg aagagtggaa
      841 aaagaaaaat caaatccaac taagatggaa tgaaaatttc attcgaaaat tcgtcaaaag
      901 tctttctttg atcaacagtg aacaagcggg gagacaggaa atagaaattt ttgttgtttc
      961 agaaagttca gtcaaacaat ttttttatcg aacccaattt catttgtatg cggaagaagg
     1021 catcgctgaa ataaatccta ttttatttcg ttcattagaa gaattgcctg atgaatgtct
     1081 atgtgcgaaa aaaagagtgg ttttgtgcga tgctgctagc tatcaaaatg gattagaaac
     1141 tgaaaagaca aagatctatc caatctcatt aaaaggtgca aacatccatt tgtatcatat
     1201 tgctcaggaa cttcacctat taaaaaaaca tatttcttga tgagacctta attaggagac
     1261 cttctacaaa gctttttaat tgtctgtaga aggtttttat ttgagtcctg attcgtgcga
     1321 agatctactt atataacaag ctttctctga aattttttta aaatgctatt gacttagagt
     1381 caactctaag tagtagaata gataaagtgt aacaaatcta ttttcattaa aagaagatag
     1441 tacggaggag agaatgtatt atgagtacag tattacatgt attggatgca atcaatagcc
     1501 ccgcagatgt caaaaaatta tctgttgagg aaatgaaaca gttggcagaa gagatgcgcc
     1561 aaaaaatctt gcaaaaggac agcgatgtag gtgggcacgt tggtccgaat ctaggtgtag
     1621 tagaattaac gattgcgttt cattatgtat ttgattcacc aaaggataaa atcgtttggg
     1681 atgtttccca tcagtcttat ggtcataagc ttttaactgg tcgaaaaaat ggttttgaaa
     1741 aaggacatta ccgtgaagtg agcggatatt ccaaccaaca tgaaagtaaa catgactatt
     1801 ttacagtggg gcacacatct acatctatca gcttagcaac aggtttagct aaagcgagag
     1861 atctgaagaa agaatcagga aatgtggtag catttatcgg tgacggctcc ctaagtggtg
     1921 gcttagcttt tgaaggattg aacaatggtg gtgttttaca atccaatcta atcgtattag
     1981 taaacgataa tgagatgtct attgatgaga atcatggcag tttatataaa aacttagctg
     2041 agcttcgtca aaccaacggt aaaagcagca ataacctatt taaagcaatg ggatttgatt
     2101 atcgttacgt ggataatgga aacgatctgg aaacggtgat ccgtttattt gaagaagtaa
     2161 aagatattga tcatccgatt gtacttcacg tccatacgga aaaaggtcgc ggttatcagc
     2221 cggcaatcga taataaaatg aaatatcatt ggcatgtacc atttgatctg tccaccgggg
     2281 aggcaaagca agtaacttct ggagaaacat ttaatatgat catcatggat tatttggata
     2341 agaaaatgca aacaagtaca gctcctattg ccgcagtcaa tgcagcgata ccaggcgcct
     2401 ttggactgaa aacattccaa gaaaaacatc cagatcatta ttttgacgtg gggatcgctg
     2461 aacagcactc tatcactttc gcttctgctt tggcttctca gggagtacgt ccagtcgtct
     2521 tccaatcaag tactttcttg caacgggcat atgatcaatt atcacatgat ttagcgatca
     2581 acgagaatcc agtgattatt ttagtcggtg gcggaacgat ctcagctagt gaccgaacac
     2641 accaaggaac atttgacatt ccttacttat caagtatccc gaacatcaaa tatcttgctc
     2701 cagcaacaaa agaggatatg atctctatgc tagattgggc gttcgaacaa acagcgcagc
     2761 ctgtagcaat ccgaattcct acacatggtg tagaacatgg tacagctatc gcaaatgatt
     2821 atagtattcc agcctatcaa acagtaaata atggaggaaa agtagcaatt ttagctttag
     2881 gcggttttct tgaattagga aaagaaacag taaaagcttt tagaaaaaaa ggaatcgaag
     2941 caacgttgat caatccagga acgatcacag aaaatgacgt agaaacatta gataaattaa
     3001 aagaaacaca cgaattagtc atcacattag aagatggtag tttgtcagga ggatttggcg
     3061 aaaatatcag tcgttattat ggtgattctt caatgaacgt attaaatttt ggtgcagaga
     3121 aagaattcac ggatagcgtt cctgtagggc agctttatga acgttatcat ttaacaccaa
     3181 aacaaatcgt tgaagatagt ctgcgtgtct tgaatattca ataattcatt atgctcacca
     3241 tccagataat tgtttatact agtatctgga tggttttttc attataaaaa gcaaaggggt
     3301 ggatgttatg tatcagcaag tagaagaata tgtcaaaaag ttccaagaaa aatatcctag
     3361 ttatcaatca gaaaaattag gtttcttttc atttggggat acagaaaaaa tggcagacga
     3421 gttgagcgaa ctagttgtaa aaaagataaa gactggtaca tcaagtggtt acgatctgta
     3481 taaacaagag aatgagcctt taccaaaagt tggtcaatta gacgtggtcc tgaatggaaa
     3541 aaatgaacca gtttgtatca tcaaaaatat aaatgtgacc atcttgcctt ttagtgaagt
     3601 cacgaaagag catgcttgga aagaaggaga aggggatcgg acattagctt attggcggaa
     3661 agtccatgct gagtttttta aacatgctta taaagaagcg ttgaacatag aatttacaga
     3721 agagaagctg gtcgtatacg aagaatttga agtaattttc ttttagtaag aaaggtctgc
     3781 tgaaagaaat agctcattat tgtctcctga atagaaaagg gttacatttt tatatgaagc
     3841 tgtattttta tataacatta ctggaggaga tattgtggat acactggaac aaatcaaagg
     3901 cggagtcgtc gtttcttgtc aagctttgga aaacgagccg ctacacagtt cttttattat
     3961 ggggagaatg gctttggctg caaaagaagg cggagcagtt ggtattcgag caaatacgac
     4021 agaagatatc aatgaaatca agcgaatcgt tgatttaccg gtaataggga ttatcaaaag
     4081 agattacgat gggtcagaag tgtttattac tgcgacagaa aaagaagtgg aagaactttt
     4141 ggagacgcaa gcagaaatca ttgcactgga tgcaacttct cgtaaacgtc cgacagaaaa
     4201 aacaactgct gaacttgtcc aaatgattca cgaacacggt cggttagcta tggcagatat
     4261 ttcgactttt gaggaagcgc ttcaagcgca agaagatggt tttgatcttt tgtctacgac
     4321 tttagcaggc tatacagaat attcacgaaa gacgcagaca cctgattttg atttattgaa
     4381 tcaaatcata gaacaagtcg atatcccagt aattatggaa ggacatactg actctcctga
     4441 acaagtagag aaagcatatg aattaggtgc tttttcagtc gtagttggat ctattatcac
     4501 ccgtccgcaa ttgatcacta aaagatatgt cgaagccgca agtaaagcaa ataactaaaa
     4561 aacacgcacg gcacagattc ttgacaaaaa agaaaaattg atagatagtt taaagcagat
     4621 tctaagaaga tgaaaaaagc attacttttc attttgtctt caagatctgc tttttaacga
     4681 attttataga aagaaggcga aaccatgttt gaacggagtc cttcagaatt gacagaacgt
     4741 gaaaattaca agttgctgat tggttcagtc atccctagac cagtagcagt agtaacgact
     4801 aaatcagcaa aagatatagt gaatatcgca cccttcagtt acttcaatat cgtcagttcg
     4861 aatcgaccca tcttatcctt ggcgatccag agaaaacaag atgcagtaaa agacacagca
     4921 aagaatattc ttgagacaag agaagctgtt atccatatcc ttgatgagga caacgtagaa
     4981 gaagtaaata aaacagcagc caatctgcct aatgaggaaa gcgaactgta cctgactgac
     5041 ttgactttaa ctgattcagt aatcatttca gtaccagcat tgacagaagc aaaagttcgt
     5101 tttgaaacat ccttatatga acatgtcgag atacggaata aaaataaagt aacagctgat
     5161 ttatttttgc tagaagtaca gaaatatcat ttaaatgaaa cagtctatga tgataagaca
     5221 ggaaggatcg atccaagaaa attgaatgct gtaagccgtt tggcaggaaa tgattatgca
     5281 gcaataggag aaattttcac gattgcacgt cccgaataaa aaggagcact agttatgtta
     5341 aaaataagct ataaaccatc gacagattca aaagaaatga aaaaagaata tgaaacagtg
     5401 aatgattttc tgcaagggca gtacttagaa gttccgccat tgcaagacca ttttgtcgtt
     5461 actactgtta ctttagatgg taaggaaata gaaatgc
//
LOCUS       DACDKL010000154         5480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDKL010000154 DACDKL010000000
VERSION     DACDKL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.156"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..985
                     /locus_tag="IV329_002209"
     CDS             <1..985
                     /locus_tag="IV329_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8941373.1"
                     /translation="IKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMNYSP
                     NYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDVVDL
                     TEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAAGVM
                     QAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYSLES
                     GLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVVSYL
                     NPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1045..2466
                     /locus_tag="IV329_002210"
     CDS             1045..2466
                     /locus_tag="IV329_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8941374.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2459..3958
                     /locus_tag="IV329_002211"
     CDS             2459..3958
                     /locus_tag="IV329_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8941375.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            3997..5391
                     /gene="uxaC"
                     /locus_tag="IV329_002212"
     CDS             3997..5391
                     /gene="uxaC"
                     /locus_tag="IV329_002212"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8941376.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1784 a         1026 c         1135 g         1535 t
ORIGIN      
        1 gatcaaagat atcgctaaag cagctggcgt ttctcatacg acagtttctc gtgctttgaa
       61 taacagcagt ttaataaaaa atgaaacaaa acaaaaaata cgacaactag ctaaggaaat
      121 gaattattcg ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg
      181 actatttttc tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag attccattgc
      241 aggcatccat tcagtattag acacaaatta cagtttatct gttgaaagta tcgatgttgt
      301 tgatttaaca gaaatcaacc tgcaacgcta cgatggactg atcgttatga gtcaaagtga
      361 tgatgatcag cctttcatcg actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa
      421 tcgtcacatc aatgatccag cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat
      481 gcaggcagta gattacggta ttggatgcgg acatacgaag atcgcttata ttggcggaaa
      541 gaaaaatttt cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc
      601 gagcatccct gtcaatctag cctatttttt aacaggagat tacagcttag aaagcggcct
      661 ccaaaacatg gaatacctat tatcactttc tgacctgcct actctggttt tttgcggcaa
      721 cgacgacata gcgattggtg cattgagagc tgtacattta gctggcctcc gtgttcccca
      781 gaacatttct ctgatcgggt tcgatgattc tccagtcgtc tcctatctca atccgccgct
      841 taccaccgta cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga
      901 tttgatcgaa ggcaggacga tcgaacaatc taaaattgag ctctccacct tgttgatcct
      961 tcgtgagtcg atcaattact tgtaaactac aagtaatttt atataaaaat gttaacgtgt
     1021 gcataacaca gaaaggaagt ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac
     1081 gaagcacctg taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg atttattgat
     1141 tggcagattc agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa
     1201 cctttagaac aaggtcttgg aaatatgatg aaccagcaag ataatttata tacggtcatt
     1261 ttacaaggac tcagcaatgg cgaaatcgtt gatacttccg agattatcac cgtagtagaa
     1321 caaatgatca atccttatga tgaatgggat gcttatctat ctctagcaga aaatgacgac
     1381 ttggcctata tcgtctccaa tacaacagag gcaggaatcc aattcgtgga gactgataat
     1441 ttaacagata ctcccccaca gagcttcccg ggcaaactga cagccttttt ataccgacgt
     1501 ttcaaattaa acaaagctgg ctttaccatc attccttgcg aactgattga cagaaacggc
     1561 gaacgtctaa aagaaatcat tttacgctac gcagaaaaat gggatttaga acctgctttt
     1621 gttgagtggt tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg catcgtccca
     1681 ggttatcctc gcgaagatgc aggtgttttc aatcaacggc ataactatga agatcaattg
     1741 atggtcaaag cagaaccatt catgctttgg gtgatcgaag ggcctaaaga attggagaat
     1801 caactaccat tcaaacaggc tggcttgaat gtcatcatca cagatgatat gactccttat
     1861 cgtgaacgta aggtgcattt gcttaatgga ccgcatacag cgatggtccc tcttgctttg
     1921 ttggcggaat tggaaactgt agaagaagta atgaaagatc ctttattctc cgcatatgtt
     1981 gatcagttat tcaatctaga attgattcct atgctttctt tacctaaaga tgaattagcc
     2041 atttatgctg accaaatcaa agaacgtttc ttgaatcctt ttgcccatca caaattagaa
     2101 gcgatttcat taaatagtgt ttcaaagttc agcacccgtc tgcttccagt gttcaagaag
     2161 tatatcgaag aacaaaatca ggtaccaccg ttaatcactg tttcactagc tgcgctactt
     2221 cttatgtatc gaggcgatca agtaaagcca catgatgatg aaaagacgat aagcgaattt
     2281 actgatgctt ggtctgataa tggaacagct attcctcgat tattgcaaaa cgctgcgctt
     2341 tggggagaag atttgtctca aataccgaat gtgacagata cagttcaaga aatagctgca
     2401 aagatcgatc aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac gatggtccat
     2461 gcttgaaacc ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt
     2521 aacccctatt ccaaaacaaa cacaactgac gattgatcag caaacgatca ccactcttga
     2581 agatatcccg caaggtcata agattgcatt gggtgatttg aaagctggag acaacgtgat
     2641 caaatatggc tatccaatcg gtcatgtgac gacagacgta actgccggtc aatggctgca
     2701 cactcataat gtaaaaacca atctttctgg tgaattggat tatcgatatg aaaaagatgt
     2761 ccatccaagt cattaccttt ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg
     2821 aaaagtaggg attcgtaatg atttgttttt agtacctacc gttgggtgtg tgaatggcat
     2881 tgctgaactg atcgtcaaac agttcaagga aaaacatcca gatctgggac agtttgacca
     2941 tattacgatt ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc atcaaaatac
     3001 tcgagaaatt ttagcggacg cagtaaatca tcccaatgcc ggaggagttt tagtatttgg
     3061 attaggttgt gagaacaata cagttcctga attcaaaaaa attttaggtg aatatgatga
     3121 agaacgagtg aaatttctag tggctcaaga tgtttacgat gagatcgaac aaggtgtggc
     3181 tctattagaa gagcttttaa cagcggcgga aaatgaccag cgcatatcag tcccgctctc
     3241 taagttgaat gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa taactgctaa
     3301 tcctttactt ggtgcatttt ctgactactt gattgctcaa ggaggcagca cgatcttaac
     3361 agaagtgcca gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga
     3421 agtttttgac tcaatcgttg acttgatcaa tgacttcaaa cagtatttct tgtcttacgg
     3481 ggaaccagtt tacgaaaacc catctcctgg taataaagca ggtgggatca ctacattaga
     3541 agataagtct ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca
     3601 atatggcgaa aaaatcagaa aaccagggct tagtttattg caagctcccg ggaatgatct
     3661 tgtagctgcc tctgctcttg cttcatccga ttgccagttg gtcttgttta ccacaggaag
     3721 agggactccg tttggctcat atgtcccaac ggtcaaggta tctacgaata caactctttt
     3781 tgaacgcaaa ggtcattgga tggattttaa tgcaggcgtc ttattagaac aaccaatgga
     3841 agtaatctta gaatcgtttg ttcaaaaaat catcgctgta gctagtggag aagaaacgaa
     3901 aaatgaacaa aatgacgttc gagaaattgc catttttaaa aacggagtaa cactttgatt
     3961 ttcataaaac agtataaaaa gaaagaagga agatttatgt ttttatctga tgatttttta
     4021 ttaaaagacg attgggcgaa aaagctttac cattcctacg caaaaaacat gcccattatt
     4081 gattatcact gtcacttaga tccaaaagaa atttacgaaa acaacaattt cagcaatctg
     4141 acagaagctt ggttaggagg cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg
     4201 cctgaagaaa aaatcacagg aaatgcttct gattttgaaa aatttttagc ttggtgtcaa
     4261 accgtaccaa aaattatcgg aaatccgctt tacagttggt cacatcttga attgaagcgt
     4321 ttcttcggta ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg gacacaagca
     4381 aatgaaattt tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta
     4441 gaagtgattt gtacaacaga tgatcccgca gacgacttgc actatcatca gttgcttgct
     4501 catgaagaac cagatttgaa ggttctacct tcattccgtc cagataaagc actcaatatt
     4561 gaaaaagaag gatttaatgc atgggtcaca ctactggaag aagcagctga ccaaacgatc
     4621 accacctatc aagaactgat agaagtctta gggcagcgga tagaatactt ccatcaacat
     4681 ggatgtcgtg tttccgacca cgctttagac atcttacgct ataaagaagc ggatgaaaca
     4741 gtattagaag agatttttca aaaagcacga gctaacaaac tattatcttc agatgaaatc
     4801 gatgcttatc ttacagaaac actgatacat ttgacccact tctatcattc tcataattgg
     4861 acgatgcagc tgcacatcca tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta
     4921 gggcccgata ccggctatga tggaatgaat gatctttctt tgactattcc tctgcaaaaa
     4981 ttattgaacc gtgcagaaga aacggatcag ttgccaaaga cgatccttta ttcattgaat
     5041 ccgaatgact atcctgcgat tttagctttg atgggctgtt tccaaaaaga acagaatgga
     5101 aaactgcagt taggttctgg atggtggtac aacgatactc gagctggtat gcgcgatcaa
     5161 ttgacacaat ttgctgatgg tagtgctttg gggaattttg tcggcatgct gacagattca
     5221 agaagtttcc tgtcctacac tagacatgaa tatttccgtc gtgttctttg tgaattttta
     5281 ggcgaaatgg tcatgcgagg agaagcacct gaagatgaac aacttttagg aagtctagtt
     5341 caagcgatta gttatacaaa tgctaaaaat tactttggct tttttgccta attgattaat
     5401 agtaacagta acgaatcaaa aacaggatag tgaaataacg gaatccgttt atccacgttt
     5461 tagagaagaa gacggaccaa
//
LOCUS       DACDKL010000155         5473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDKL010000155 DACDKL010000000
VERSION     DACDKL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.157"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            67..294
                     /locus_tag="IV329_002213"
     CDS             67..294
                     /locus_tag="IV329_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941377.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            574..2397
                     /locus_tag="IV329_002214"
     CDS             574..2397
                     /locus_tag="IV329_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8941378.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            2411..3820
                     /locus_tag="IV329_002215"
     CDS             2411..3820
                     /locus_tag="IV329_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8941379.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            3838..4674
                     /locus_tag="IV329_002216"
     CDS             3838..4674
                     /locus_tag="IV329_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941380.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            4739..>5473
                     /locus_tag="IV329_002217"
     CDS             4739..>5473
                     /locus_tag="IV329_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941381.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKN"
BASE COUNT         1710 a          907 c         1204 g         1652 t
ORIGIN      
        1 acatttgcta gttaattttt aagaatacta gcccaaatca ttctatcgat acctttatat
       61 ctcggaatga ttgaaattgg ctattgctta ctgattttta ccaaaaaaac aagtatcact
      121 attgttgcct atattgttgg aatgctgttt attccatcct ttactcacca aattcatcaa
      181 tttttcccaa gagcagaatt gttgaagttg tgtgatccgt tatatgcatt tttgatgctt
      241 tcacgatttt gggaatttcc cgctggttat gtaggaatgg ttttactggt atagttaata
      301 ttagatatac ttattttagc agtaagtata cgcagatata ccctagttga tgtgatctaa
      361 ctgttatatt accctatacg gtcaaacgaa aagataaaaa attaaattcc ctgttgattg
      421 cgctatcata atgtgctatt attggattac agcaaaataa cgttattggc atgttactga
      481 ttatgcaggc aagaccaaga caaactaaca cttcaatgtt gattggggcg tggtttgact
      541 tggtcttttt tactgcttta aggagagaga ataatggatt acagagaagt aggaagaaat
      601 gtattgcaac aggttggggg aaaagaaaat gtggtcagct taacccattg tgccaccaga
      661 ctgcgttttg aactcaaaga taaaaggaaa gccgatacga aagccttaga aaaaacactt
      721 ggtgtaatta gtgtggtgga tagcggtgga caataccaag tggtgattgg caatgaagtc
      781 caaacagcct tcaaggaaat tcaaaaggaa gtgggaactt caattaaaaa gggcccctct
      841 tcagacaatc aagaaagtat tatttcaaga ttgattagtg tcatttctac cacctttaca
      901 ccgatgattc cagctataac aggtgccggg atggtaaaag ccattttggc cgtcttgact
      961 ttgactggtg tactatcaga cagtagtcaa acttacatca ttttgaatac tattgctgat
     1021 gcagcctttt attttatgcc agtactatta gcctatgggg cagccataaa gtttgatgtc
     1081 aacccaattt tggcgattac tgttgctggc attttgcttc atccaaatat ttcacagctt
     1141 ttttctactg gcgatgccgt ttcttttcta ggaattccgg tattagctgc agattatgcg
     1201 ggatcagttt taccgattat ctttactgtt tggatcatgt cctatgtaga gccctttgcg
     1261 gatcgaattt cgccttcgtt tattaaattc tttactaagc cgatgctggt atttttaatt
     1321 atggggcctt tagcgttggt tgtcattgga ccgtttggaa cgttgctgaa cgatcttata
     1381 gcaatggggg ctgaatggat taacggcaaa gcagcttggt taattccatt tttgatgggt
     1441 ggtttgcagc catttttggt ggttacgggt acagcttggg caatgacgcc aatcgctact
     1501 atgcaattga gcagtacggg ttctgaaatg atcaatgggc caggaatgct ggcttctaat
     1561 attgctcaat caggtgctac ctttgctgta gccgcgaaaa ctaaaaacaa agagttgaag
     1621 cagctagctg tgtccactgg gatcactgct ttaatgggaa ttactgagcc ttcactctat
     1681 ggagtaacct tgaagctgaa aaaaccatta gttgcttcca tgattggtgg tgcaattgct
     1741 ggtttgtatg ctggcttgag cggtttagtc agatatgcct ttgtttcccc tggcttagca
     1801 gccttaccag cctttattgg agaaaatcca atgaatattg ttcacgcaat tattacgtgt
     1861 gtaatcgcct ttgttgcaac ctttatcatt gcttatctaa ttggatttga cgatacttct
     1921 gatgaatcag agcaaacggc aatagccgaa gcagtcattg ttgaaaatcc ggtaaaagga
     1981 accgtgtgtc ctatttctga agtgaacgat gctgtctttt ctaagggact attgggaaag
     2041 ggcgtagcca ttcatccaga agaaaacagc atttattcac ctgttgaggg agaagtcatt
     2101 actattttag aatctaaaca tgcgattggt ctacgttcta aagaaggtat tgaattattg
     2161 atccatgtgg ggatcgacac cgtgaattta aaaggggaac cctttgaagt atttgtaaaa
     2221 gcaggagatc aggtgtcagc aggacaatta ttaatcaacg ccgatttcgc tgccattaga
     2281 aacgctggct acgatgatac agtgattgtg gtagtgacca acagtgaaaa ttacgcagga
     2341 attagtgaag caagcggaag cttaaaggaa cacgaaccat tgatgcaaat tagttaagga
     2401 gaggattttt gtgcaataca aacagctttc agcatttcca gagaagtttt tatggggtgc
     2461 ctccattagt gcctaccagg cagagggtgc ctatcaggag gatgggaaac aaccgtctat
     2521 tattgacaag tatcagcata cagaaggggt agctaatttt gaagttgcct cggattttta
     2581 ccatcactat aaagaagata tacagctgtt taaagaatta ggcttaaaag cttttcgctt
     2641 ttccatcgct tggacgcgaa ttatggatat tgaaaataat aaaaccaatc aaaagggaat
     2701 ttctttttat catcaagtaa ttgatgaatg cctgaatcag ggaatcgaac cgattgtcac
     2761 catgtaccat ttcgatttac cttattttct agaggagcag ggcggttggt taaatcgagc
     2821 aactatcgat tattttgtgg actatgtaca ggtattgctt actgagtatg gtgacaaggt
     2881 gaactattgg ctgacaatta atgagcaaaa cacgatgatc cttcatcctg gagcgattgg
     2941 tttgccacca agtggagagc tgccttcaaa aaaagaactc tttcaaatta atcaccatgt
     3001 tttattagct caagctagag taattcactt gtatcatcag ttgaatctta aaggaaaaat
     3061 cggtccagca atcaatttaa cggctatgta tcaagctacc tccagtccag aagatgcaat
     3121 tgcagcccat aattgggaaa cattgagagg ctggtctttc ttagatgtag cagttcgggg
     3181 aaaatacaat tatttgtttg aaaactattt gaatgatcgt ggattatatc ctaaaattga
     3241 ggcagaggat caggcaattt tatcctccgg taaacccgat tttattgcga ttaattatta
     3301 ttcaactgcc accatcgctg ccagcaaaaa tgacggatca gatgtttccg ctagagcggg
     3361 ggatcaacag atcatgttgg gagaagaagg tgtctaccgg gcagctgaaa atccttatgt
     3421 agataaaacg ccatatggct gggtagttga ccctactgga ctacgattaa cattgaggaa
     3481 attgtacgat cgctatgatt tacctatttt aattactgaa aatggctatg gggcacccga
     3541 catagttgag gaagatagac gaattaatga ccaggatcga attgattatc tagccaaaca
     3601 tattctggct attcaagcag ctttaacaga tggtgtagat gtttttggtt atttaccttg
     3661 gtcagcgatt gatgttgtta gtacccatca aggcttcaat aagcgttatg gttttatata
     3721 tgttgaccga accgatgaga atcttaaaga attaaaacga ataaaaaaag aaagcttctt
     3781 ttggtatcaa aaagtaatcg caagaaatgg cttagcttag agagaacggt gaatctaatg
     3841 agagtaatta aaacgttaaa caacagttta attctttgct tagataagca aggcaaagaa
     3901 gtaattttga tggggaaagg gattggattt caactaaaga aaggcgattt ggttccggct
     3961 gacaaaatcc aaaaagtatt taaattagat tcactttctg atgctctaaa aaaggattac
     4021 attaaactgt ttgaggctgc tccggatgat ctaattattt gcgtcaagcg aattatcgat
     4081 tatgccaacg ccaagtttga ctttaagctg aataaaagtc tattttttac cttggttgat
     4141 cacttgaagc atgccattga acgcagtcaa aaaggcattg tttttcaaaa tcgcctgctg
     4201 attgaaattc aaaagtatta cccaaaagaa tttgagatcg gctgctatgc tatccaattg
     4261 atcaacaaaa aaatgaatgt tcagttgcca gaagaggagg ctggaaacat tgcgtttcac
     4321 attgtaaatg cccaaagtga acaagcaaat atggaagaaa ccatgctctc ggtaagagca
     4381 ctaaaggata tcttggctat gctaggatat ctgtttcccg aaaaaaaggt tgataaagat
     4441 tcgatgcttt acttaagatt tgtgacgcat ttacagtttt ttattgcccg aatgattaag
     4501 aaggaagagt tgccggagaa ggatgatttt ttgctggctt cagtgaagca gcgttttccg
     4561 aaagagtatc gggcggcctt gaaaatcaag gagtacgttc aagaagagat gggtgcctca
     4621 gcaaatcagg acgaactgct ttatctaaca ctgcatattt caagggtgta ctgaatgaag
     4681 aaaatatcgg ctgcacaata tataaagact tgttggttgt gctaaggctc ctgattcctt
     4741 ggaaaattct tctaacagtg cggaggtgaa tgtttcgacc tctaccagtg aagtggtaaa
     4801 gtgttcacaa gaaactacta gcttgccaga agagctactt ggagattggc agattaataa
     4861 taaggatgct ggtatagaga tatcagacag taaagctgta ttccgtatag gaaggtctca
     4921 agtagagtca aaggattatt atgtagatgt aaacaacaaa atgcttttac atgtctattt
     4981 ttcttttgag tatgatagtg tgaaatacat tgaagtctct attgaaagaa ctggttatag
     5041 tgaatttatt agtaaaagcg tgggaatttt aactatgcac actaaagatg gacaatatat
     5101 aagtttgggt gtgaagagag tttccggtga gaataatgag acagaagcag ttacggagca
     5161 gacgatggat gaaagaatcg attcatctaa gagtaatgag acagaagtag atactgggaa
     5221 gacgatagat gaaagaattg attcattcat cgaaacatac acagctatac cacctacttg
     5281 gaagcttgta aatgaagata aaggaatcac gataaatact gatacagaga tttctacaga
     5341 tgctcaatct ttatcagatt tattagctaa tctaacaggt atcagtaatg cagtgacaga
     5401 gcaagttggt aaagacattc cacttcggtt acttgaaaat gggaaaaaag attattctgt
     5461 ggtatttaag aac
//
LOCUS       DACDKL010000156         5438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDKL010000156 DACDKL010000000
VERSION     DACDKL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.158"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            233..1342
                     /gene="pdhA"
                     /locus_tag="IV329_002218"
     CDS             233..1342
                     /gene="pdhA"
                     /locus_tag="IV329_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8941382.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1345..2322
                     /locus_tag="IV329_002219"
     CDS             1345..2322
                     /locus_tag="IV329_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8941383.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2347..3990
                     /locus_tag="IV329_002220"
     CDS             2347..3990
                     /locus_tag="IV329_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8941384.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            3998..5404
                     /gene="lpdA"
                     /locus_tag="IV329_002221"
     CDS             3998..5404
                     /gene="lpdA"
                     /locus_tag="IV329_002221"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8941385.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         1807 a         1027 c         1244 g         1360 t
ORIGIN      
        1 gcagatgaaa aataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
       61 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
      121 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
      181 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
      241 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
      301 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
      361 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
      421 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
      481 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
      541 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
      601 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
      661 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
      721 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
      781 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
      841 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
      901 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
      961 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
     1021 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
     1081 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
     1141 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
     1201 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
     1261 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
     1321 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
     1381 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
     1441 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
     1501 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
     1561 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
     1621 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
     1681 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
     1741 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
     1801 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
     1861 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
     1921 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
     1981 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
     2041 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
     2101 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
     2161 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
     2221 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
     2281 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
     2341 aaagtcatgg cttatcaatt taaattgccg gatatcggtg aaggaatcgc agaaggtgaa
     2401 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
     2461 gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat
     2521 gtgattgttc cagaaggtac cgtagcaaat gttggggatg tattagtaga aatcgatgca
     2581 ccaggacacg aagacaatga aggggacagt ggtgtagccg cagaatccca aactccagct
     2641 aaaccggctg ctgaacccac tgttgataca gaatcagctg gatcgtcaag cgaaggagtc
     2701 ttccaattta agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg
     2761 ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac
     2821 aaatcagtag aagaaattcc gtcacctgtc acagggacag ttaaaaacgt gattgttcca
     2881 gaaggcactg tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc tggacacaat
     2941 agtgcaccgt caactagtgc gccatcagct gaagcaccaa aagaaaaagt ggaaacatca
     3001 ggttcagcaa gtgtcgttga agctgccgat ccaaataaac gtgtattggc tatgccatct
     3061 gttcgtcaat ttgcccgtga aaaagatgta gatatctcac aagtaactgc aactggaaaa
     3121 ggtggcagag taacaaaaga ggacatcgaa aacttcttgg caggtggtcc aagcagtgca
     3181 ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag aagcagctcc tgcagcagaa
     3241 agcaaaccag ctgcaccagc aaaaccattc aagtccaact taggagactt ggaagaacgt
     3301 gtagcaatga cgccaacacg taaagctatc gctaaagcaa tggtcaacag taagcataca
     3361 gctccacacg tgacattgca tgatgaagta gaagtgtcta aactatggga taatcggaaa
     3421 cgatttaaag aagttgctgc tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg
     3481 aaagctttaa cagcgactgt taagaaatac ccagtattga atgcatctat cgacgatgca
     3541 aaccaagaaa tcgtttataa acattactac aatatcggta ttgcaactga tacggatcac
     3601 ggactatatg taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc tattgcagac
     3661 gaaatcaatg agaaagcaaa attagctcat gacggcaaac tatctgcaga agatatgcgc
     3721 aatggaacaa ttacgatcag caatatcgga tctgtaggtg gcggttggtt tacaccagtc
     3781 atcaactatc cagaagtagc tattttaggt gtaggaacga ttgcacaaca accaatcgta
     3841 aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac
     3901 cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata atatcaaacg tcttttagct
     3961 gatccagagc tattaatgat ggaaggatga taaacaaatg gtagttggag attttgctgt
     4021 agaattagat acagttgtaa ttggtgcggg acctggtggc tatgtagctg ccatccgcgc
     4081 tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag tatattggcg gtgtttgttt
     4141 gaatgtcggc tgtattcctt caaaagcact gatagctgca ggacaccatt atcaagagtc
     4201 actagattct gaaatgtttg gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac
     4261 acaagaatgg aaagaaaaca aagttgtcca tacgttgact tctggtgttg gtttcctatt
     4321 aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg atcacacact
     4381 acgcgtcatt catccagatt cagctcaaac ttattcgttc aataacgcaa tcatcgcaac
     4441 aggttcccgt ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt tagattctac
     4501 tggcggatta gcattgaaag aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat
     4561 tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg
     4621 ctcaccacaa attttaccaa cttacgaaaa agatctagta aaattagtag aagatgattt
     4681 caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg
     4741 tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa gaatccgtta ctgctgatta
     4801 tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat atgggcttag aacaagcggg
     4861 tgtcgaagta ggagaacgag gattgatcac ggtggataaa caaggacgta cgaacgtacc
     4921 taacatctat gccatcggtg atatcgttcc aggggcggca ttagcacaca aagccagtta
     4981 tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag attataaagc
     5041 aatgccggca gtagccttca ctgatcctga attggcttct gtaggtatga cgatcaaaga
     5101 agcaaaagat gctggtatcg aagcaaaagc atataaattc ccattttcag ggaacggtcg
     5161 ggcattatcg cttggtaaaa cagaaggatt catccgtcta gttacgacga tcgaagataa
     5221 cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct ctgaattagc
     5281 tttagcaatc gaatcaggaa tgaatgctga agatatcgcc ttgacgattc atcctcatcc
     5341 atctcttggc gaaatcgtca tggatgcttc ggaattagca ttaggtttac caatccatat
     5401 ttgatttgaa taatacaggc gaaacgcaac tgaaaaat
//
LOCUS       DACDKL010000157         5437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDKL010000157 DACDKL010000000
VERSION     DACDKL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.159"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(88..690)
                     /locus_tag="IV329_002222"
     CDS             complement(88..690)
                     /locus_tag="IV329_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8941386.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            935..1141
                     /locus_tag="IV329_002223"
     CDS             935..1141
                     /locus_tag="IV329_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941387.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1453..2172
                     /locus_tag="IV329_002224"
     CDS             1453..2172
                     /locus_tag="IV329_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8941388.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(2253..2936)
                     /locus_tag="IV329_002225"
     CDS             complement(2253..2936)
                     /locus_tag="IV329_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8941389.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3154..5340
                     /locus_tag="IV329_002226"
     CDS             3154..5340
                     /locus_tag="IV329_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8941390.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2022 a          904 c         1047 g         1464 t
ORIGIN      
        1 ttttttaatt tttcagggat tttgaattca gaactcatac caaagcagca gccagataca
       61 aaaaagtcag ataagttagt taattgttta atgattgttt ttttcatgaa agattcctcc
      121 aaaattcatc aagtagtttg taataggaag caacgtagct atttctaaaa caatcccaag
      181 tagcaaatag gtagaaaagt aagaagctga gaaaaataaa gtaatgagag aaaaaattag
      241 aaacatgctg atcgccttct ttaataatgg gggacgtttc tcgggttcaa tgggattgat
      301 tgctgtacct cttgatccat agcgaagcag taacaaaaag cagctgatta acaaaaagac
      361 catcattggg aaggagatag tcaattgcag atgtttcatc accaagggaa cgccccataa
      421 ataaagtagt gtccacagca gacaaacgaa ttcagagtcg gaatgcaatc cataagcata
      481 tctgcggatg cagatataag aaagaaaggt gattcctgtt tccgcccatg agcctgtcac
      541 cagagaaaag actagaacga ccgctagttt tccaagatta ttcaataaaa cttctaaggc
      601 aaattttact tggacataca ccatatcttc ttccgatttg tctccgctga ataaggcttc
      661 taataatttg ttcacaactt tatctttcat ctcatacgta cctccaccac aaattttatg
      721 ctaaaaaaaa taaaaaacgt cttttgtttc ataaaaagtg gttttgacat caaaaccgtc
      781 atgatctcct aaaaaaatct tcgttttcca agaaccttac caaaaaaagt gacagttttg
      841 atacgaaaag tactttttat gacaagtttg tgctttttcg agtgatttat gttaaaaaat
      901 tactgacctt ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt
      961 cggcgaaaca tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga
     1021 aaaaatctat gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga
     1081 taccgctcat aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata
     1141 aaaacagata aaatgttgag agcataaaga ggttgtgagt ctcccgttta gctctgagtt
     1201 acaaggaata atgttaaaaa acagcttttc atgttttatg aatattgcct tgtgaccgag
     1261 gtgttggcag gagaatactg tttatttgaa aaaagggctg cgacaagact tttgtcacag
     1321 cctttatttt catgaatagg agttactttt ggctactttt agagaagtag cttttaagga
     1381 gcagataaag tcaaggcttt tatttctata aatgaagtag taaaatgaag aacagatttt
     1441 attacaggaa ggatgccgat aattgtgatc attgttgcag gaacgatcgg tgcagggaaa
     1501 agcacactaa ctgaaatgct tgcccaagat ttagaaacaa aaccttttta tgagaacgtg
     1561 gaagataatg aagtattgcc gctattttat tctaatccag aaaagtatac ttttttatta
     1621 cagattttct ttttgaacaa acgctttttg gcaatcaaag atgctttcag ccatgatgac
     1681 aacgtactag accgctccat ctacgaagat agtatgctgt ttcatttgaa tgcagattta
     1741 gggcgtgttt ctgaagtaga agtaaagcag tatgaaggtc ttttagaaac gatgctgaag
     1801 gagttagaag aaatttcgcc acaaaagaaa ccagatctac tggtttatat tcgtgtatcc
     1861 tttgaaacta tgttagcgag aatcaaaaaa agaggccggg aatatgaaca gctagaacaa
     1921 gatcctgaac tctattccta ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa
     1981 cagtttgata tatgtcccaa aatagtgatc gatggggaca aatatgattt cgtagccgat
     2041 ccggcatgtg gagaacaaat cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa
     2101 gaggcagatg cccagtcgat ccgctacact acaaataacc cgaaagcaaa gggaatcttt
     2161 acaaataaat aatataaaaa ctaagcaaat tgctcactcg cagatatttc tctgtgagca
     2221 gcatcttgct tagttttttt gattcattga gatcaaatag tgaaccgatt cgtatgataa
     2281 aattgccata aatgaaatgt ctgagtgagg gccatgatca aaaataaaat ggaaacgatc
     2341 aaaatcgaat gggcgccaaa cagatgatga cagaaaacaa ggacaatagc tcctgcgata
     2401 aaggcaagaa cgacacttag atagtggaaa aatcgatcaa aatctgcttt ttctttagaa
     2461 atacaataat catataagtt cgttgctacc ccttttaaat ttccagttgt aaataaatta
     2521 gtgtaggcac gcccgttgat tttatcaaaa gctacccact ggatcgatgc agtaaaagca
     2581 atcaaaatcg ttactaatgc gggatgctca tggacgggtg tcaaactaac aaagaaaaaa
     2641 atcactgctt cataatatag aacaaacaag cgccagaaat gtttttcttt ttttctaaaa
     2701 taatcaatta gatactttgt tagaagaatc cctaagaaaa agaaaagggt agacaatact
     2761 tttttaccaa cacttagcca ttctccgtca aatgcttgga tgatcgatag aatgatattg
     2821 ccagtttgag cagaggcaaa agcaccatat tggatataag tataactgtc catggctcct
     2881 ccgataaatg tcagcaaaag tcctattgta cgcccctcgt gaaaggaagt ctgcattttt
     2941 ttcatcacct tttatgaata tttttgtctt ccttatctta gcataaacga ttgaaaaaaa
     3001 ggaaaaattt tcagaaaact aaaatcatat cgcttcccat tttcattttt agaaaggtct
     3061 gttagtataa agaaaaaata gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat
     3121 tcaaagaaga aagatgggaa ggaaatcaat cagttgaatg aaaaagaaat tctccgatta
     3181 caaaaagaat tgaaacgctg ggagaacaag tataaaaagc ttcaacaagt ctttcggcta
     3241 caagaagaaa tcattgctac ctatgaagcg gcttatggcg aagcacccgg acttattgag
     3301 gaagaagtac aagtaaaaga aagtgaagga aacagctctt tctttaaaat gatggagaaa
     3361 cgttcctata atcgcttcaa tcaagaacaa aaagcagcta tcacatatga tatgaagaaa
     3421 catcttcgaa tcatagcagg agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct
     3481 gtttatctga tgacgcaaaa acaagtagac gaagaacgca tactaatgat cacttttaca
     3541 aggaacgctg caaatgaact aaaaaaacga gtagataatt tcagccagag aaaaaccgct
     3601 gtccatatcg gaacattcca tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa
     3661 tttcctgaag tagcaatgca aggaatacaa ggagactttt caaaggatac tgcacaaaaa
     3721 gtgaatgctg ttttgcaaca gctgatacgt aaatataatc tttatatatt tgatcaatat
     3781 ggagaaaaaa cgattgcttc tcggttagac tattggcaga atatgaacct ttcagtagaa
     3841 gaaatggttc agttagttaa agaaaaatat gattccattg ataagaatgc acgacagccg
     3901 atatcagaac gactgtatgg attgctcacg gaactccaag aacaaaaaag aagccagcaa
     3961 ttgctagagt tcaatgatgt actccaaaat ctgaaaagcg ctttggagaa tgaggagatc
     4021 cagcattata tcggccaaaa atatgattat ctattcattg atgaatttca agatactaat
     4081 ccacttcaat gggaaatcgt gaaacggatg acaaaggaaa acaggatcaa gttgattgtt
     4141 gttggagatg atgatcagag tatctatgga ttcagaggat cagagccagc atacatcaag
     4201 aactttgaaa aagaatatcc taccaaaact cttttcttat tgactaatta tcgttctcgg
     4261 gcagctattg tccaaggagc aaatagattg atcagctata ataaaaatga tcggatacca
     4321 aaggcgatga ttccagcaca aaaagaagca ggtgttatgg aagcctattt gttttctgat
     4381 actaaagcag aaagtcagtg gctaatcaaa caaattcaga catttattca aaaaaatggg
     4441 aaatataagg aatcaatcat attataccga tcagcttcac aaacgcagca gcttgtccaa
     4501 tctctgctga aaacgaatat tccttttgta ctggaagcag atagtccata tgaaggtatc
     4561 tttggtatca agtctttcca acatttctat caaaaaattg ttcattggca aacagcactg
     4621 aatctgcaaa agaaaaatca ggcctaccag caattgctcc ggcagctgat ggcagactgc
     4681 tacctgaaaa aaagtgaatg cgatcaatac ttttctccta aatatcaaaa tatagccgtt
     4741 gcagattata ttttgtcgat aagccccaat ttaaaatcga aatctgcaga aatccgagag
     4801 tttgaggaag cgttgcgtaa aacagaccag catcaagaaa tctattcgct tgttcaaagc
     4861 tatcttcatc tgccacgtat agctaaggaa atcgaccaga gtgatcaaga atggatacga
     4921 gaagaagtaa agcagcatgc tactttcaga agactccaag ccttatcgca agaaacccaa
     4981 gcgacagcta aagaattaaa aacccgcttg attgcttaca gacaaggaaa acttgatgca
     5041 ctatgtatcc agagcattca caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt
     5101 ggttgtaacg aaggaagttt gccttataac ggagcgacag aaaagaaagt aatcgacttt
     5161 aatgaaataa aagcagaacc agtgacgacg atagaagaag aacgaagact attttatgtt
     5221 gcgatgacta gagcaagaaa tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga
     5281 aaattgaaag tatcgagatt tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga
     5341 tagaagagag ggtgtgacag aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa
     5401 ttgcttctca aatttttgtg aaatttcagc ttatttt
//
LOCUS       DACDKL010000158         5252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDKL010000158 DACDKL010000000
VERSION     DACDKL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.160"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            108..1397
                     /locus_tag="IV329_002227"
     CDS             108..1397
                     /locus_tag="IV329_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8941391.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1523..2602)
                     /locus_tag="IV329_002228"
     CDS             complement(1523..2602)
                     /locus_tag="IV329_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8941392.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2711..3151)
                     /locus_tag="IV329_002229"
     CDS             complement(2711..3151)
                     /locus_tag="IV329_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8941393.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3208..3618)
                     /locus_tag="IV329_002230"
     CDS             complement(3208..3618)
                     /locus_tag="IV329_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8941394.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3799..4233)
                     /locus_tag="IV329_002231"
     CDS             complement(3799..4233)
                     /locus_tag="IV329_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8941395.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4333..5181)
                     /locus_tag="IV329_002232"
     CDS             complement(4333..5181)
                     /locus_tag="IV329_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8941396.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1930 a         1012 c          878 g         1432 t
ORIGIN      
        1 atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc
       61 tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa
      121 ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc
      181 acacagagga gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa
      241 ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac
      301 aaacagcgac catcaaagca aaaattgact attcaggaga cttagcacct atactacaat
      361 cagctgttac atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt
      421 tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt
      481 taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt
      541 taacgccgta tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag
      601 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
      661 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
      721 aaactcaacc agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag
      781 atgtagaccc agaagaaaaa ccggatgtga ctccagaacc agatacggat tctggaaacc
      841 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag
      901 aggcagcccc agaagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc
      961 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaactag
     1021 aggtagatcc agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc
     1081 aaggaatacc agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta
     1141 aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa
     1201 tatttgatag cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgcta
     1261 gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac
     1321 tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac
     1381 aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa
     1441 atatccagaa aacgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac
     1501 tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga
     1561 tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc
     1621 cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca
     1681 gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa
     1741 tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga
     1801 aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa
     1861 tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga
     1921 gcaacgtgtt taaaagagcg ataacaaatt gagcttctaa taccaaacca aatgtattga
     1981 caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa
     2041 acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga
     2101 atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg
     2161 aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt
     2221 gtaaaacagg atctgcatct tttggctgat gctcgtaaaa atcaataacg gactggatca
     2281 tatcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt
     2341 aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct
     2401 gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg
     2461 taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc
     2521 gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac
     2581 aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag agaaagagca
     2641 aaaagaaaaa ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca
     2701 tctagcagtt tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca
     2761 gatgttgcag tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg
     2821 gaataccttc tgcttgtttt tgcaacgcag caccttgagg agtcagcgta acatttacga
     2881 ttctttcatc acccgtttca cgctgacgat taatataatt tttttctgct aactttttta
     2941 gtaagggagt cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag
     3001 tctttttctt ttcccataaa actaacatca caagatactg ggtataggta agattcagcg
     3061 gcttgaggag ggaagtgtag tgtctaatga tttccttaga ggcggcatat aaaggaaaac
     3121 atacttgatt aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg
     3181 tatcatagcg gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat
     3241 atttccagct gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg
     3301 cgtttcttcc agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct
     3361 cgtttcactt tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt
     3421 tttttgctgt ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa
     3481 caattgttca gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc
     3541 gaatgtacga tcgggattat gaacttctcc agagcgtccg cctgtgttga tcattttcgt
     3601 agaatacatt tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta
     3661 cacaatataa ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac
     3721 atgaaacaga cgcgctttct cgaatagcaa acatatacaa aaaaatatca gagacaaaaa
     3781 ctaatctcat cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaact
     3841 tacttcacga tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga
     3901 gttagtcaca ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg
     3961 taatgcttta ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt
     4021 tggatgttca gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa
     4081 tctcgtgcca tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc
     4141 aatcagttgt atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt
     4201 taatacaaaa tctatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa
     4261 cagttactag attcttttat tataccagtt aaagaaaacg cattcaaaaa aatagcggat
     4321 ttacttacca actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac
     4381 gctgctgaca atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga
     4441 aaattcatga tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc
     4501 ttttgcctct agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc
     4561 ttccaaataa tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat
     4621 ataagtcatg acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat
     4681 tccaaaaaca aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga
     4741 agtattcaag tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga
     4801 agagttgttt ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac
     4861 agacaacaac cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa
     4921 aaacaagctt ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag
     4981 taccaacata acgatattca acactaaaat aactatagct cggtcaaatc ctaaaacagc
     5041 ttccagtaat attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa
     5101 catattgata gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat
     5161 aaattgtctc gtttctttca tttatccact tccttaattc attaataata ctcttatctt
     5221 attataactt acttttcacc ttaagttaac tt
//
LOCUS       DACDKL010000159         5232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDKL010000159 DACDKL010000000
VERSION     DACDKL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.161"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            170..703
                     /locus_tag="IV329_002233"
     CDS             170..703
                     /locus_tag="IV329_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941397.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            complement(705..896)
                     /locus_tag="IV329_002234"
     CDS             complement(705..896)
                     /locus_tag="IV329_002234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941398.1"
                     /translation="MSKTTKINTKNKPDLYNCPPLSILTIIATAIANIKIRIVAICGF
                     FLVNVFNDFNDLDIKNITP"
     gene            1240..1620
                     /locus_tag="IV329_002235"
     CDS             1240..1620
                     /locus_tag="IV329_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8941399.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1602..2516
                     /locus_tag="IV329_002236"
     CDS             1602..2516
                     /locus_tag="IV329_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8941400.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            2476..2754
                     /locus_tag="IV329_002237"
     CDS             2476..2754
                     /locus_tag="IV329_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941401.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            2761..3450
                     /locus_tag="IV329_002238"
     CDS             2761..3450
                     /locus_tag="IV329_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941402.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3571..3657)
                     /locus_tag="IV329_002239"
     CDS             complement(3571..3657)
                     /locus_tag="IV329_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8941403.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            4333..>5232
                     /locus_tag="IV329_002240"
     CDS             4333..>5232
                     /locus_tag="IV329_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8941404.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALS"
BASE COUNT         1882 a          918 c          989 g         1443 t
ORIGIN      
        1 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
       61 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
      121 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
      181 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
      241 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
      301 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
      361 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
      421 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
      481 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
      541 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
      601 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
      661 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
      721 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
      781 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
      841 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
      901 aagtctaaaa aaaaataaat cgatacattt tagaagaata gggttgtttg aatattacaa
      961 agcaacaatt tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc
     1021 tagccttttt ttgtattgtg gagcgtggat tttgaggggg attcaagggg gagcttgcgc
     1081 tcctttgaca agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc
     1141 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg
     1201 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca acatcaaaat
     1261 ttggctagcg atcattctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     1321 cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt
     1381 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     1441 ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa
     1501 attgccaagt ggtgcaatac acaccaacac gacgttgctc ctgatgatgc tcaacgttta
     1561 tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa
     1621 gtcgaaccac ttcatgtagc cgagcaatca attacgcaga accacgagca accgtcaaaa
     1681 caggaataaa ctgtgacatt gattatgcca aaagtgaaat gaaacaaata cgcacgcttt
     1741 atggaaaaga cgatcgtgta caagcacact tattgattca gtcctttcga ccaggagaaa
     1801 ttaccgctga aaaagccaat caactagggg cagagtatgc gaaaaaaata gcccctaacc
     1861 accaaatcgc gatttacaca catacagaca aagaccatat tcataatcac attgtcatta
     1921 actccgttaa tcttgaaact gggaaaaaat ttcaagccca tgggcaagca tttttaaaca
     1981 aatgttatga cctaaatgac gagatttgtc ttgaacatga cttaacaatc acggaacgag
     2041 gaaagaaaac cgaaaaacga accatgtctg aaattaaatt aaaagaaaaa aatgaaccgg
     2101 tttggaaaga cgaaattcgg tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa
     2161 cgtataccca attttgtgat tcacttaaaa cattcggcat tcattgcttg aatcgcggaa
     2221 aaaattttac ttacgaattg gtccacaaaa agaaaaaagt acgatccaac aaactaggaa
     2281 aggattacga aaaggagacg attttacatg agctggacag acgagaacga acctacaatc
     2341 aacaacgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag
     2401 tacgaagaga acaacgaact tttacgcctg aacaatcgga aattagaccg actcgaagaa
     2461 accatgaaca acacgttgaa cgaggaacag accaccaaca agaattatcg ctctaaaacg
     2521 ttaaaagaca tcgaaaacga aataaagaga caacgtgcct ttcttgcgcc ttatacccaa
     2581 gaaaggatac aaactacgat ggattcacaa gtcaaaaaac tccaccaagc gctattagac
     2641 cagcgaaacc aagaacaaaa aaaggcttta attcaacgat cgatggatat tcttgccccc
     2701 ttatgttgct tagtactaac attattagcc ttttggttgg tatggggaga ataaacagac
     2761 atggcattct atttacccta tctactcatt tctgccagta ttttaggaat tatttggctt
     2821 agctataagg cttatcaaac aagatattca ttaaaaggct ctaaaattcg atttaagcga
     2881 tttttattgg tcggggttat atttgcctta tcaatcgttt taagtggttt attcggcgtt
     2941 ttagagggta ataaacgtgt ttcaagaagc attttattag gggatatcac tcaaaagtat
     3001 gaagcagcat gtgataaaaa gaaaaaagag caagcattag cacaaaaaat ggaggaattt
     3061 accgcttgtt atgaggacat gaacaatatt tttgtggatc aagagaaacg tttaacagat
     3121 aaaaacatga aaaaactcac tcgtttttat caaaacttac cagaaaaaca gcaaaaagag
     3181 gtacaagaaa aatatgagca agtaaaaaaa gacgtgcaat attttaaaga tacgcagaca
     3241 gaagaagctt gttgtaactt atttagcgat acaatccctt tttcaaccag tgaacaagaa
     3301 cgaaaagaac ggcaacaatc ggtcacatac gaacgttatg aaacactgct tcagcaagca
     3361 acaaacatac aaaatccaac gaaaaaagaa acggcattaa attatctaga aagtgtcaag
     3421 gaatggttgg atcagcaaca acaaaattga atttgattag caatcatgtt aaactgaacc
     3481 tgcaaatgat caaacattag atacacgaaa gccccagaga ggtcggattc tctggggctt
     3541 tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat tcaaccaata
     3601 actgaataaa gagacacaaa cttctacaag aagggggcaa atgaaccgtt caaacattag
     3661 atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat accttacaaa
     3721 ataaaatctg tgtggtgttt ttaagtatcc cttattttcg ggttgttagg gcttgccctg
     3781 accgcctgta aggcgcttta agaacaagat aaggtttttt acccaagact gtcttaaaac
     3841 gacttagacg agctatcaga ggcttccccc acctccgtta ctgacttgtt tttagcaaag
     3901 gagcctttag gcgactgcgc agtacaatgt gagagcatag cgatcacact tttttagcga
     3961 ccgtagggag caaggttttg aattggcagt tttgaaaaat gcgcgaacct gttggtagaa
     4021 aaaagaagtg gtttttaggc gggaaatttt gtcaccctct tttataatct ctttttaaac
     4081 ctttttttaa acctctttac cgtcggctgt gtccttactc cttcaatgga tacagcgttt
     4141 tataaactac attttgtctg cttttaaact acattttgtc tgcttttaaa ctacattttg
     4201 tctgctttta aactacattt tgtctgcttt taaactcttt ttttgcactc acgtatgtta
     4261 taatatgcat tatcacagac aaaacgttgt ttataacgtc tgtatccatt gaaggagtaa
     4321 ggaggaatat ctttggcaaa tgaaatcgtg aaataccatc atgaattaaa taccattccg
     4381 ttgcggaaat ttacaccagt tgaaatgaat ctatttttct ctattgtgtc tagaatgcgc
     4441 gaaaaaggcg ataaaacagt ccgtcttact tttgaccaac taaaagattt aagtaattat
     4501 aaagcaactg ctaataatcg tttcattaat gatttagaaa tgacttatga taaattaatg
     4561 gatttacgtt ttggtaaaag aagtgctaat gggctcgata tagatagatt cgttatgttt
     4621 agtgagttta aaatacgtgg aactgctaaa gatccatatg tagacataca agtttacgaa
     4681 aaagctttac ctttactaaa caatttagat gagtgggttc gctattcact ccaacaattt
     4741 aacgaattac aaagtagcta tgcaaagact atgtttcgat tactaaaaca atatcgaact
     4801 aaaggttttg cttatttttc taaagaggat tttcatgaac tattagatat tccaaaaagt
     4861 tactggaaca accaagcaaa cgttgataat cgtgttataa agcctatacg acaggaatta
     4921 accgcaatct tcaaaggatt atcaatcaaa aagaaatacg gaaaagggcg aggaaaacct
     4981 gtcgttggct atcaatttac gtttaaacca gaaatcaaaa aagcagatga ttttaataaa
     5041 catgcaccaa ccaaagaaga acaaaataaa gcattggctt tagctgataa acaaaaagaa
     5101 tacgaagaac ctgctacaac aattaacgaa aaaatcaaac agaagaaacg agaacttgaa
     5161 ctcctccaac aaatcaaaca actagaaaaa gagaaagaag ctcttgaaaa acaaacagag
     5221 tccgcacttt cg
//
LOCUS       DACDKL010000160         5222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDKL010000160 DACDKL010000000
VERSION     DACDKL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.162"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            246..1553
                     /locus_tag="IV329_002241"
     CDS             246..1553
                     /locus_tag="IV329_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8941405.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            1674..2873
                     /locus_tag="IV329_002242"
     CDS             1674..2873
                     /locus_tag="IV329_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8941406.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2900..4168)
                     /locus_tag="IV329_002243"
     CDS             complement(2900..4168)
                     /locus_tag="IV329_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8941407.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(4338..4979)
                     /locus_tag="IV329_002244"
     CDS             complement(4338..4979)
                     /locus_tag="IV329_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8941408.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
BASE COUNT         1620 a         1094 c          948 g         1560 t
ORIGIN      
        1 ggatcttatg catttgatta tgattttcag tttattaagt attatcagtt tatttgcttt
       61 cagcgcttcc cgcttattag atgcggaaac acagcctatt cctgtaaaag tacgtaaaaa
      121 cgaccgctaa ccttccataa cgtgcgttct tatcctaaag ttttttcaga aacattgaaa
      181 ctctctcccc tctctgataa aattttaatt tgtagcgaaa caaattaatt acaaggagat
      241 cggatatgtc cataaaaaaa acacgtctag tcctattatt ggctagtctc attctttgcc
      301 ttgcttcttt tctaccagct gttgctgtgc aagctgaaga tactttcaaa gtaaatgcaa
      361 aagcagcctt tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa
      421 aaaccatggg gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag
      481 taaaagaagg gaaactatca tgggatgata aagtttcaat ttcagattat gcagaaaatt
      541 tgagtgtcac cccagattta tcaaatgttc ctttgcataa agaaaacacc tacaccgtta
      601 aagaattatt cgactctgca atcatccagt cagccaacgc ttcaatggtt gccttggcag
      661 aaaaaatctc tggcagcgaa gcaaaattca ctgaacgaat gaaagaacaa ttgaaagatt
      721 ggggtatcaa agatgcaacg atcgttaatg catcaggact gaataactct tatcttggcg
      781 aaaaccgacc agaaggtact ggagaaaacg acgaaaatca aatgtctgct caagatgtgg
      841 ctatcgttgc ccgtcatttg atcttggatt ttccagaaat actggatgta tctagcacta
      901 cgacacaaat gtttggcgaa aacacgcaat caccagtaga aatggtcaac tggaactgga
      961 tgctcccagg attcgtgaat tataaagaag gcgtggacgg attaaaaact ggtacgactg
     1021 aacttgcagg agcctgtttc gtcggtacga tcactaaaaa cgatcaacga atcatcactg
     1081 tcgtattgaa tgcagacggc catgcggaaa acccaagtgc ccgtttcaat gaaacagctc
     1141 gcttaatgga ttattgctac gacaattggt cagaaaaaga gcttggaaaa gccaatgcaa
     1201 gtatccctag tctaaaaacc attgatgtaa aagacggaaa agaatcaaca gtgcccgttg
     1261 tgcttaaaag tcctgtaaaa gtatgggtac ggaacgatat ggatactgga aaattaacaa
     1321 tcacaccaac tcttgacaaa aaacaagtca ctgacaatga gatccaagca cctatggata
     1381 aaggaacaaa aatcggtacc gcaacgatca ccttggcaga agataaatta ggttatcttg
     1441 aagacaacaa cgcgccatcc acagaaatca tcacggataa aaccgtagaa aaagcaaata
     1501 ttttcgttat tggctggcga cgcgtcgcag aatttttcgg taacttgttt taattgaaca
     1561 aaaagaaaaa agcatctttc catgtggaag atgttttttt gctttttcta gtttaaataa
     1621 cagctttttt catctatttt cgttatactg tataagaaaa aggagttgaa tttatgaatc
     1681 taaccagaga tctgattttt ttggttctct atcttattgg ggtattctcc atcgtttatt
     1741 ggcgaaaaca gaaaaaggat ccttccttgt tcttttacag cagtacatgg acgtttgcgg
     1801 taatcttgac ttattttgtc gtgttggaaa ttttttattc gaccttttat tttttgattc
     1861 ccttagtgtt tttcagtatc tttgcctttt cctatttttc agaaaagcga aggctgttga
     1921 atggtctttt attcaatatt ttcctcatta gttttggtat ctacttgttt gtcttgctat
     1981 acgaaacaca aaatattttt cttggcggac tgattgcctt gatcacgatc ccgttactgc
     2041 ttgtctttct attcggcatt tatggattga tcgtttttct tttttggaac ggtgtcacag
     2101 ttttacgtag agagtcgcat tcattagcta atcttttaac gttgatacta gccatttttc
     2161 ttacattgtt tcttgttttt gactttttct tgttaaaata tttaccacaa tggataaatg
     2221 ctttgttttt ctgcatccca ttgatcttga tctatctatt tatcgtattc tacaactttt
     2281 tgactgtctc attcttgtat cagttcaacc gccctcgata caaccaagac ttcatcgttg
     2341 tgttaggcgc cgggttgatc aatggagaaa cagtttctcc tcttcttgcc aagcgaatca
     2401 ataaggcgat tgctttttat tgggcacaaa gccgtgcgac attaaatccg cctatcctat
     2461 taatgtcggg tggacaggga gcagacgaaa aagtaccaga agcaatggcg atgaaacaat
     2521 atgccatgga acaaggaatt cctgaaagag atattcttgt cgaaaccaat tcgacaacca
     2581 ctttggagaa tatgcgctat tcaaaggaaa tcatggatca gcaaatgaaa ggtccctatc
     2641 gcgcgatctt cagttccaac aattaccaca ttttcagagc tggtctctat gcacgacaag
     2701 cgaaactgaa ggcaaatggc attggggcaa aaacagcttt ctattacttg cctaacgcgt
     2761 ttttaagaga atacatcgcc atcttgatga tgaacaaaaa aagacatatg atcatttgcg
     2821 gattgctttt cgcaggttcc gcattcttat cactgatcac tttacttttc taaaaaaaca
     2881 aggtgtgaca aaacatatgt cacaccttgt ttttttcttt tattctcttg acgattggca
     2941 agaatagtat tccttttttg acggctactt gctgtgcaga ataaataccg ctgtttcctg
     3001 aacacataaa gctgatcaag cagatcataa agaagtaagc agcagcttgt gaaccaaata
     3061 attcgattcc catcacaaaa caggcaatcg gtgtatttgt cgcaccagaa aataccccga
     3121 taaaacctaa tccagcaaga aaaggaacag aaacatggag caaaggcgct agtactgcgc
     3181 caagcgttgc accaatctca aatagtggtg tcacttctcc cccttggtac cccgcaccta
     3241 aagacaagac cgtgaagaat aatttcccaa taaaatcata agcatgtgcc tgtccattaa
     3301 atgattcctg cagtaacgga agactcaacc caaggtaacg ttgtgtttgc aaaatcaacg
     3361 ctgcggctac aactactgct ccacctatcg tatttcgcca aacaggatga ggaatccatt
     3421 gcgcataccg cgcttttaaa aagacgatcg aacggctgaa tacccaacca atcaaaccaa
     3481 aagcaatccc agcaacaaaa actttgaaga acagcaacat actccattca ggaatcacac
     3541 ccatttggta atgtagatga gaaacaccat agctttctgt catgaaattt gcaaaaagcg
     3601 cagcaaagaa gctaggaaat attgcttctg ttcggacttt accgattgcc aacacttcta
     3661 atccaaataa tgttccagct aaaggtgtgc caaatacaga actaaaaccc gcactgattc
     3721 cactgataac caaaatctcc cgttctgctt tatccaatcg gaataaatgc gctatattat
     3781 cagctaatgc tccccccatt tgaactgctg ttccttcacg accaacagat cccccaaata
     3841 aatgggtagt gatggtccca aacaaggtca atggaatgag ccgcaaaggg atcttctctt
     3901 ctcctccatt cccttgatcg atcactaagt tattcccacg actagaaaga ccgccatgat
     3961 acgcataaag ataagcaaaa agcgcgccgc tgactggcaa tagataaagt agccaaggat
     4021 gtgctagtcg gatatctgtc acgaacgtca ggctatttaa gaaaaaagca gacaagctcc
     4081 ccatgaatac tccgagaatc gctgtgacca ccagccactt caacatgtac atcaaaatac
     4141 gcactggact cggtaattgt ttttccataa aaaattcctc ctcaaataaa aaaacgccta
     4201 ctaaagaaag gggctggccc cagactttag taggcgtcat tagctatctt acgttagcat
     4261 ttaaaggcga acaccatcac ctattcactt ccattaaaag atagcatatt tttctgtaaa
     4321 aaacaataaa attatcgtta tttctttaca cgttcagcaa attcatagaa aggagccaat
     4381 ttcgttaact gttgattaca ttgatcacta tcatgacaaa tataattccc ctttttcgta
     4441 tacgtgccat cagaaccggc tttcgttgta gaaagaaaga gcgagacatt cgatgttttc
     4501 tgacagatcg cacagattcc cttattaatc gttggtgata aagtaccgct gatccctttt
     4561 aattttccat cttcgtaata aaccatgaat tttttttgcg tccctggatc attccaaccg
     4621 atgtacgtat gtgcttgcaa gtcgatttca ttccaagcgg gttgtttgag ttttttcact
     4681 tttcggaata atttctcgat ttgtggtttg ctcggctgca taaacggctg gacgatttgc
     4741 ttcagctcct ccaatgctcg ttctgcttgc gtttttgtca gttttacatc taaaactgtc
     4801 gttaaaaacg tatcgatttc tgatggttcg taacctaata catcgcgaat cttttcagct
     4861 gctagagcct gaaccgtttg taccgttttt gagtcattga cagatgagta cgcatttaat
     4921 aactgctcta cttctctttt tataaaaaaa tattgatatg gtttcattgt ttgttccatg
     4981 attttaaagt cctttcttct aaccaaagtg catactttgt agaaaaaagt gatcttctgc
     5041 aaagtatgtg ttacctgaag atcccggact cttttaaata agatacgttt tgcatcttat
     5101 catcaccccc tttaatgttc attcttctat gtacatgatt cattcgataa ataaaagtaa
     5161 accgtatctt gagcagatag tcaactgcaa acagaaaaag gactgtgaca aaagtcgtgt
     5221 ca
//
LOCUS       DACDKL010000161         5194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDKL010000161 DACDKL010000000
VERSION     DACDKL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.163"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(172..393)
                     /locus_tag="IV329_002245"
     CDS             complement(172..393)
                     /locus_tag="IV329_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941409.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(390..680)
                     /locus_tag="IV329_002246"
     CDS             complement(390..680)
                     /locus_tag="IV329_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941410.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(677..1861)
                     /locus_tag="IV329_002247"
     CDS             complement(677..1861)
                     /locus_tag="IV329_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8941411.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(2043..3446)
                     /locus_tag="IV329_002248"
     CDS             complement(2043..3446)
                     /locus_tag="IV329_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8941412.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3468..4241)
                     /locus_tag="IV329_002249"
     CDS             complement(3468..4241)
                     /locus_tag="IV329_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941413.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4251..4628)
                     /locus_tag="IV329_002250"
     CDS             complement(4251..4628)
                     /locus_tag="IV329_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8941414.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4649..4963)
                     /locus_tag="IV329_002251"
     CDS             complement(4649..4963)
                     /locus_tag="IV329_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8941415.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         1518 a         1035 c          889 g         1752 t
ORIGIN      
        1 ctgatatttt cattttagaa acaatctcgt tacctccttc ataataattt gaagagctct
       61 ttctttctaa tgttttaatt aaaagagatt taaattgatc atttgtaaac atattcataa
      121 atcctttcat aacaaaatat atacttccta ttttatcatg aaaaatatct gttaagcacc
      181 aatgattcgg ttaaataagt ttaataacac atctttcacg ccagctgcat tgaacacaag
      241 tccgacggcg attagagcaa taatcaagaa gccaatgagt tttgaaaatt ctcgtttaaa
      301 acctaaaaac agtccaatga caacaatcgc taacaatacc aatgactgtg cattatccaa
      361 gaaccagttg tacaaattct gcccaaaatt catgtgattg tcctcctttc cttaagatat
      421 ttttctcgat aatagatata gctttttaca cgccttaata attgaaaagg ggttaaatgc
      481 tctccataga cggttactcg attggtataa ttattataaa atctcacctt gtaaaaatca
      541 taggtataga tggtatcacc agcttgccgt tttccctcaa taatgagaga cgtatatttc
      601 cctgtatttt gtatatacgc ttgaaactca atgccacact gaaaccctaa cttctttaat
      661 tcttttgtta aaaggctcat gtaatcaaat cctccatggc tagatgttgt tgttcaatta
      721 atttctcatg tttttctgtc agtttggctt cctgaatcat ggtggagata atggttgtac
      781 cgttgagaac atctaaaatg cttgcgattt ttaatgtagg ggcgacttga tgatgtaacc
      841 agttcaatgt tcgttcaaaa gaataaggct ctggctgtgt ggttaggcgt aatgctccac
      901 gatttttacc gataaaccat tcccaacgtt cattagtttt ccaatcactc cgacgtttag
      961 tgtcgtcttt atctgcaaag cggatatagc gattgataat ttcaaaggct gttttttccg
     1021 cctgttgatg tgccaataaa tctttcatgg cttgggtggc tcggtcattt tttaaccgaa
     1081 tttcaaaccg attctttact tctgcgtctt ctaagggaac atcatttttc acgtattgct
     1141 cataatcttt ctcatatata cagaaataga cttcactttt taatgagcca atataaagtg
     1201 tgtttcccat gtatggtttt tcatcacggt gaaccaattc accactgcga taatttttaa
     1261 agcttcgaaa gacggaaatg cattcttcct gtttacattt ctttgccaaa tctggaatat
     1321 tcaaaatccc tgttttatcg ttgatagcta aatccaagcg tttgaaaata cctttatgtt
     1381 ccatgcaatc ttgaaagaaa tcataccagc tccgatgttg agccaataga aagttttcaa
     1441 attgccgaca gccttttcct ttcagttcta ataaaacacc tttacttaca tcatgagaga
     1501 ctaggacaac aatatcaccg aaacgatagt gttcgggata agaataatag ccaaagtctt
     1561 cgtgtagcat ataatccata ttcaaacgta aaacctcacc gataatttga gccacatcat
     1621 tggtagggaa tcgaatccgt acatagtcga ataacatctc caatgggtta tcgggattga
     1681 agcgttccaa agcgttcagt aaatcttctt tcacctgttg agtagggact gccttgcctg
     1741 tttcaatatc cgataggtaa ggtctggtaa tatgagaagc aaccgccagt ttattttgag
     1801 aaacgccata agccagtcgc ttttctttca attccttaat ccaaattttt tcgttttcca
     1861 ttcgcaaacc tccaatctaa aaaagatgta aactatgacc cttgagttta cacctttcat
     1921 attttccgca atcctttgta ccatcaacat tggttgatga gaattgcctg ttttcagttg
     1981 tatttgtgcc cccctgttag atagaggggg ctaaaggatt ggcgtggcta acgccacacc
     2041 aatcaaagca agtctgaacc tgtacctttc acttcgtgcg gtacaggctc agattgcttt
     2101 ttttgaatga cttttcctat ttctcctaag aaatcgtacc tttttggtac aaggggtgta
     2161 taaaactcac taatcacact ttttcctgta tccacgtaac ctcttccctt gatgggtttt
     2221 aagaaaaatt gtttttgtgt atcgcttccg aacatcatac caaagcccat ttcagacata
     2281 cgacccaaag ccacacggaa gttaaagttg tcccgaatcc catcactaaa atattttgcg
     2341 tctggacgtt gacaggcaag aattaaaaag aagcctgctt gtcgtcctaa cattacaatt
     2401 tgtttgagtt tatgaataac ggtggtactt tcacgaccta gcatatccat cattgccaca
     2461 tattcatcaa aaataagaaa attggcagac aaaccaagat aagcataatt ttctcctgtt
     2521 ttatagttag ggtgttgttt catggtttca ctgcgatcaa tcatatcttc gtaaaattga
     2581 ttgatacatg ccaacatatc ttctttacgg taatagacat tattcatgac cgcccctaag
     2641 tctgccaaat cagcattttt agggtcaaga atataaagct ttgcatttgt atgcaggaga
     2701 gattcaatca atgtgagaat aaaataggat tttccactac cagtgccccc agcaattaac
     2761 atgtgaggga gagaatcgaa ctcccaatag accgattcca tcaatttgac acgtccatct
     2821 tgagctacca cgtcttcaat gccaattcga ttgccaatag tatcataaag taggacatac
     2881 tctacataag aatctttcaa cgttttttcc actaattcac aatacaatcc tgtttctagt
     2941 ttgttttcta ggtgcaaaag ctgatcttga tatttcccta aggtgatttc tacatgaata
     3001 tggattagtc catctttcat tcgataatat attttaggaa aatggcggat tcgttgtttg
     3061 gttttcttgc tgggtaaatc tttaaagaat ccctctgatt gagtttcttc ggcttcatac
     3121 caatgatttt ctaaaatcat acgagctaac ttttgacggt gctctaactg ttttatagaa
     3181 tctatacgat agtgataata aagccagctg ataagtccgc atacaaaaag agcagtgaga
     3241 atactgctcc aaaagtatgg cgtatgaagt tgatagaaaa atgtatctaa ctgaactgct
     3301 tgccagttca ccgattgtaa cgctctccaa tgaaaaggga gtaggacgac tagaaaaact
     3361 accagcaatg aaattagtac aaaatgatag accaagtgct tatcgttggc tttgattcgc
     3421 tctcctctgt catgaagcaa tggcataaaa atcctccttt ccacctatca aaataattgt
     3481 gtattgtaga tacgagcctg tttatcaaaa gttggttgag aaatggcttc atatttctga
     3541 taagcgattc gcttttcaaa gcgtaaattt ttttctttcg gataataatg acgtcgatag
     3601 agagtgtgtc ctaacagttt tttcgcttct tttgtcaacg gatagagata gcgaaacatg
     3661 cgtccattga ttttctcaat ccctttgtat tcgcaaaatg cctgggaaag ccaatgcttt
     3721 ttctctacct gttcaaagcg agcattttcc tctaataaaa ggcgagcact tcgagggtgt
     3781 atcttttccc atgactgctt atcacgatag acacttgtct tgaaataccc gcagtaaaag
     3841 aagttggacg cttgatagac atagccacat tttccctcaa ttccatctgc cagtgtatat
     3901 aaaaaatgac agtccgtatg ttctttcaac cacttaatta gagctgaaag agcttgactg
     3961 ccaaaataat tggtttgatt catttctgga agaaaacaca tctttccaat ttctaaataa
     4021 tctgtggttt gaagggaact gtcgggaaag agtttgcgta tggtttgtag gggttgcgta
     4081 ccccagccta attccacaac gcctaataat ttttcttctg aaaaaatccc tagaaagtat
     4141 ttacacaatc tagggagtat ctttgaataa tggtattgcc gaataaaaga aatggcttca
     4201 tccttcggaa tttctttcaa aatcaatggt gtgtttgtca taagcaacac ctatttttca
     4261 ctttcggacg cttctttttt atatttctga tgaggttttg tctgtccttt taatacaaga
     4321 tcgtctgctt tcacgtacca atcaacgtct gcccctcgaa aagtagccgt cgctacggta
     4381 tctgccacag gattgattaa ttccacttct gcgttgtagt caaattcttt caaaggaaca
     4441 gaagcgggga tactgacttg tatcatgcgt ccttgtcctt ttgattttaa gtcataggta
     4501 cgttctttaa tttcttcgga aaccgtacca tcttcattct ggatacgaac ctcacgacgc
     4561 aaggcggaaa atttcaatgt gccaaatgtc tggtcttttt caatcacaat accttctgct
     4621 aatctcatat tttttcacct ttcctttctt atgctttcac catatcgtct gcgtgcataa
     4681 tgtaattggt aaatcctcga ttgccaattt tataaccttc tgcggtaata tgtgggttga
     4741 tgagtttgac tttttcgtcg gattcaaaat gtttctcgcc agcttcggag ggaagaatga
     4801 caacaatatc atctgctcgt tgaatcgttg agtataaatt gtaactacga gagagaatgg
     4861 ttggtctgcc atttattcga cgttgttcaa tgttcccttc accagcatat tctaattgtc
     4921 caaatgtttt ttccatattg ggaatgacat atttaagttc catattttta cctatccttt
     4981 ctgtttttat gtttgaatga atcttatttg tgtgatggtt ttaaaggggg gagagcgacc
     5041 ttttgattct tgatttttta ttttcataag ttcacttcct ttcaaaattg ggtaaaaaaa
     5101 tagacgctcc gtactgagcg tccgattcct actatattta aaacaacgac tacctaggta
     5161 ttttaaatat tataatcaaa ttctatttta tcta
//
LOCUS       DACDKL010000162         5147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDKL010000162 DACDKL010000000
VERSION     DACDKL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.164"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1341
                     /locus_tag="IV329_002252"
     CDS             <1..1341
                     /locus_tag="IV329_002252"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8941416.1"
                     /translation="DILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQF
                     NGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFAS
                     EAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDN
                     VKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPP
                     YSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFR
                     GAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREF
                     VKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEE
                     EPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1331..2548
                     /locus_tag="IV329_002253"
     CDS             1331..2548
                     /locus_tag="IV329_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8941417.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2561..>5147
                     /locus_tag="IV329_002254"
     CDS             2561..>5147
                     /locus_tag="IV329_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8941418.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLIDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYE"
BASE COUNT         1795 a          888 c         1072 g         1392 t
ORIGIN      
        1 gatattttgg gatatgcaat tgcaccagag tatcttttca acgtcttagc ggatcaagca
       61 aaacaagcaa cgttccaatt gaacgatcta aacaaagcct ttgtgcaatt ggcatctacc
      121 tacaatcaat ttaatgggtt atttgatgat gtggatctcc aatcaaaaaa actaggaaca
      181 gatgagcaac aacgaaatgt gacgattacc gaagtcatca agaaattgaa tgatgtagat
      241 gttttagaac acgatgggga cgtgatcggg gatgcgtatg agtttctgat cagtcaattt
      301 gcttcagaag cagggaagaa agccggagaa ttttacacac ctcatatggt ttctgatatg
      361 atggcgcaaa tcgtaacgct cgatcaaaag gaacgacgat tctttagtgt ttttgatcca
      421 acgatgggtt caggttcatt gatgttaaac gtacgtaact acttaacaca cccagataac
      481 gttaaatatc atgggcaaga actaaataca acgacataca atctagcaaa aatgaacttg
      541 attttacatg gtgtcgacgc cgaagaaatg aatctccgca atggggatac gttgaataag
      601 gactggccaa cagatgaacc ttacacattt gatgcagtag taatgaaccc accttattcg
      661 gctaattggt cagcagatac gactttctta gatgattctc ggtttaatcg ttatgggaaa
      721 ttagcgccaa aatccaaagc agattttgcg tttcttcttc atgggttcta tcatttgaag
      781 gaaacaggta ccatggccat cgtcttacca cacggagttt tattccgtgg cgctgcagaa
      841 ggggtcatcc gtcaaaaatt actagaagat ggcagtattt atgctgtgat cggtatgcct
      901 gcgaacttgt tctttgggac atcgattcca acgacggtga ttgtgttgaa gaagaatcgt
      961 caaactcgag atgtattatt cattgatgct agtcgtgaat ttgttaaagg gaaaaatcaa
     1021 aataaactat cggaagaaaa cattcaaaaa atcctcgaaa cctacgctga aagaaaagat
     1081 gtcgagaaat acgctcattt ggcaaccttt gatgaaatca aagaaaatga ctataactta
     1141 aatatcccac gctatgtaga tactttcgaa gaagaagaac caattgatat ggttcatgtc
     1201 ggaaatgaca tcaagaagat acgacaagaa cagcaagtgc tagaaaaaaa acttttagaa
     1261 gctatctctt ccctgcaaac aacacctgaa aatgaagcgt ggttacaagg tgcgctggag
     1321 gtgttcaaac atgagcaatg atacacaacc agaaatccgt ttcccaggat ttacagatga
     1381 ttgggaacag aggaagttgg gggacttcgt tgtagattat gttgaaaaga caagtgtaca
     1441 aaatcaattt cctatgctaa cttcatctca acaaaaaggg atagttttgc aagaagacta
     1501 ttttgcaaat cggcaagtaa ctacagaaaa taatattggt tactttgtac tacctagagg
     1561 atatttcacg ttccgtagtc gaagtgataa tgatgtgttt gtgtttaacc gtaatgacat
     1621 tattgataga gggattataa gctattttta tcctgtattt actttaaaat ctgctgattc
     1681 cgacttcttc ttaagacgta ttaacaatgg aatccaacga caattatcta ttcaagcaga
     1741 gggaacaggt caacatgttc tgtctctaaa aaagtttaaa aatatagtag caatgtttcc
     1801 tagtgaggaa gaacaacaaa aaatcggcac cttcttcaaa caacttgacg acacgatcgc
     1861 tcttcatcag cgtaagttag atttgctgaa ggaaacgaaa aaaggattct tacaaaaaat
     1921 gttccctgaa aacggagcaa aagtgccaga aatccggttt cctggtttta cggaagattg
     1981 ggaagagcgt aagttgggag aattaggtaa aactcaatcg ggaattggat ttcctgatgc
     2041 cgagcaaggt ggttctgaag gtactccatt ttttaaagtt tccgacatga ataacattgg
     2101 taatgagtat gaaatgagaa atgcaaatca ttatgtatct aatgagcaaa ttgagcgtaa
     2161 aaaatggaag ccaataaaag atgtgccagc gattatattt gctaaagttg gcgcagcaat
     2221 aatgcttaat agaaaacgtt tggtcacaag ccctttctta atagataaca ataccatggc
     2281 ttatttattt aataatactt gggatatcta ttttggtaaa atattgtttg aaactataaa
     2341 tttgccaaga tactcccaag taggggcatt gccttcttat aacagttctg atattgagaa
     2401 tatcagtgtt aaggttcctg taaaggatga acaacaaaaa atcggtactt tcttcaaaca
     2461 gctagatgat acgatcgctc ttcatcaacg taagttagat ttactaaaag aaacgaaaaa
     2521 aagcttctta caaaagatgt ttgtctagga gggaagaaga atgaaaatta gtcaccgtga
     2581 ggaagcagaa gtcgaagaac agttgattcg tgtacttgga gaagggcata atcagtggac
     2641 gtatcgtccg gatctaaaat cagaagaaga cctttgggtc aatttacgcc aaaaaattat
     2701 ctcaaataat caagcagaac tgaatgattt tcctttgatc gataaagaat ttgaaacgat
     2761 caaaacggaa ttactgctgc gaacgaaaac cccatttgat gctgcgaaat ggttaaaagg
     2821 tgaaaatggg atggctcgga tcacgattga acgggaagat tcgcaattgg gttctgtctc
     2881 acttatcctt tattccaatc aagacattgg tggaggaatc tctacttatg aagtggtcca
     2941 tcaaattgca aaacgtggaa gtaacataga agcacgggat cgacgttttg acgtcacctt
     3001 attaatcaat ggattaccga ttgtccaaat cgagctgaaa caagtaactg caaaagatgg
     3061 tgtttatcag gcatttaacc aaatcaaaaa atatgcagaa gaaggtatgt ttcggaacaa
     3121 tatcttttcg accttacagt tgtttgtggt ttccaatgaa caaaccactc ggtattttgc
     3181 aaatgcctta ccgaaagact tgcaccctaa gtttttattt agttggcgaa cgaaagacaa
     3241 tgaaaaagta gaaaacttat atgagttttg taaacaagtg ttaaatatcc cggatgctca
     3301 tcgtttgatt gcggattata caatcgtcag tgaagatcaa gacaataaaa ccctcatggt
     3361 gttacatcct tatcaagtac atgcgattca agccttgttt attgccgcaa ataaacatca
     3421 atcagggtat gtgtggcatg cgactggttc aggaaagacg ttgactagtt ttgtatctac
     3481 taagctttta gcacgaaaat cggggatcga tcgaacgatc atgttagtgg accgcaaaga
     3541 tttagataat caaaccacca cagagtttac aaaatttgct tcggaattta atactgggat
     3601 ttcttccggg aacgccaaag cgaatagctt gatcgttgga acgggaagtg ccaaagaatt
     3661 aagtgaaacc ctgttggcag atgccaatgc aaatgttgta atcattacaa cccgtcaaaa
     3721 actagatgcg gcgttgaaat atgcaaagaa acaagaagag aaaaaaggaa ccaatcgctt
     3781 ccaaaaatta atgggccaac atatcgtgtt tgtagtagat gagtgtcatc gagcgttaag
     3841 tgctgaaaat atggaagaaa tcaaaaaaat gtttccgaaa tccacttggt ttggctttac
     3901 aggtacaccg atttttcctg agaatcggaa acaagccaaa gggcaattgg cacgtacaac
     3961 ccatgatcag tatggagagg tcttgcatac ctataccatt aagaatgcgc tagaagatgg
     4021 ctctgtctta ggatttcaag tagagcatga aaacaccgtg gagccgactt cactagagaa
     4081 taaaatctat cggaaattaa aagaagtaga aacatatgca gagtattcat cagaacagat
     4141 caatcgtatg attgatcaaa tggaacctgt caaaaaagaa agttatttgg acccagctgt
     4201 ttttgaagca gatgaacata tccaaaaagt cattcataaa atgttccgtc cagataatgc
     4261 ctatacaaaa tttgattttc gaaatggcag gccaacgaaa tctgcaatct taaccacgag
     4321 ttccattgac atggccaaaa agtattatcg ggccatcaaa gagatgacga aagagcctga
     4381 ttggttaaca aaagagtttc ccaatcagct cattcgtgaa ggacgaacga tggaggatcc
     4441 tgattttcca cgaatcgcta ttacctattc attagaagaa aatagccgag attcaagtgc
     4501 ccaacaagaa gaaatgcaaa aaattattga ggaatataat cagtattatg atacttcctg
     4561 gtcattagca gacattgagc gttataacgg agacatcaat aatcgtttgg cacgaaaaag
     4621 agcggaattc aaacaatttg ggaaacaaat cgatttagcg attgtagtgg atcgtctatt
     4681 aacaggtttt gacgccccta cgattcaaac cctatttgtg gatcgtaatc tagagtatgc
     4741 aggattgatc caagcttttt cacgtacgaa tcgtacgtat cctgataaga caaaaggatt
     4801 gattgtgacg tttcgtaagc cagcgacaat ggagaagaat gtcgaggatg cgacaaaact
     4861 gtattcaaaa gcaaaagaag aatcaggttt gatttatcca agttatcagg aatccaaaaa
     4921 aagattcaaa caagcatatg gtaagttaaa agaggtttca tccatggaat ccattgatga
     4981 acatacgcca cttgaaatcc gtatcgagta cgtcaaagca tttcaagaat tgaatcgtgc
     5041 ttatgaatcg ttagtgactt atgacgatta caatgatgac atggtgaaat ctccaacttt
     5101 gaacaatcaa attcaacttt tggaagaaca aattggcgtg tacgaga
//
LOCUS       DACDKL010000163         5099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDKL010000163 DACDKL010000000
VERSION     DACDKL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.165"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..474)
                     /locus_tag="IV329_002255"
     CDS             complement(16..474)
                     /locus_tag="IV329_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8941419.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(487..1812)
                     /locus_tag="IV329_002256"
     CDS             complement(487..1812)
                     /locus_tag="IV329_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8941420.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1829..3241)
                     /locus_tag="IV329_002257"
     CDS             complement(1829..3241)
                     /locus_tag="IV329_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8941421.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3258..4022)
                     /locus_tag="IV329_002258"
     CDS             complement(3258..4022)
                     /locus_tag="IV329_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8941422.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4126..5001)
                     /locus_tag="IV329_002259"
     CDS             complement(4126..5001)
                     /locus_tag="IV329_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941423.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1535 a         1083 c          751 g         1730 t
ORIGIN      
        1 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
       61 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
      121 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
      181 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
      241 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
      301 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
      361 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
      421 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
      481 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
      541 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
      601 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
      661 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
      721 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
      781 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
      841 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
      901 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
      961 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
     1021 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
     1081 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
     1141 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
     1201 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
     1261 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
     1321 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
     1381 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
     1441 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
     1501 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
     1561 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
     1621 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
     1681 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
     1741 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
     1801 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
     1861 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
     1921 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
     1981 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
     2041 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
     2101 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
     2161 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
     2221 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
     2281 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
     2341 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
     2401 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
     2461 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
     2521 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
     2581 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
     2641 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
     2701 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
     2761 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
     2821 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
     2881 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
     2941 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
     3001 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
     3061 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
     3121 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
     3181 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
     3241 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
     3301 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
     3361 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
     3421 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
     3481 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
     3541 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
     3601 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
     3661 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
     3721 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
     3781 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
     3841 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
     3901 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
     3961 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
     4021 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
     4081 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
     4141 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
     4201 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
     4261 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
     4321 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
     4381 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
     4441 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
     4501 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
     4561 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
     4621 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
     4681 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
     4741 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
     4801 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
     4861 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
     4921 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
     4981 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
     5041 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacata
//
LOCUS       DACDKL010000164         5094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDKL010000164 DACDKL010000000
VERSION     DACDKL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.166"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(80..742)
                     /locus_tag="IV329_002260"
     CDS             complement(80..742)
                     /locus_tag="IV329_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8941424.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(752..1567)
                     /locus_tag="IV329_002261"
     CDS             complement(752..1567)
                     /locus_tag="IV329_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8941425.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1828..2271)
                     /locus_tag="IV329_002262"
     CDS             complement(1828..2271)
                     /locus_tag="IV329_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8941426.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2302..2415)
                     /locus_tag="IV329_002263"
     CDS             complement(2302..2415)
                     /locus_tag="IV329_002263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8941427.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(2405..2743)
                     /locus_tag="IV329_002264"
                     /pseudo
     CDS             complement(2405..2743)
                     /locus_tag="IV329_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2731..3108)
                     /locus_tag="IV329_002265"
     CDS             complement(2731..3108)
                     /locus_tag="IV329_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8941428.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3332..4525
                     /locus_tag="IV329_002266"
     CDS             3332..4525
                     /locus_tag="IV329_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8941429.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4691..>5094)
                     /locus_tag="IV329_002267"
     CDS             complement(4691..>5094)
                     /locus_tag="IV329_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8941430.1"
                     /translation="VSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILNYLTDLEE
                     LFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQHPEKNLE
                     DYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1550 a          963 c          882 g         1699 t
ORIGIN      
        1 tttcaaaaaa tgaaaaagaa tgacagtttt tacataaaaa aaactgtcat tcttaattga
       61 aagtttcaat ttataaagtc tacagatcca tagttctcat caatttacga ttgttgaagt
      121 aaatcaaacc aaatacgata gaaggaccta gtagaataca ccattggggc tttacgaaaa
      181 agaagagaaa caaacttgtc aatagtaaaa aatctagtaa actcacatac cattttttca
      241 acgtatagta gaacgctatc cgtagaactt gatttggttt cgacgctgga tttctcacct
      301 gaaaatacca gcagttggtg ctaaaggaaa ctgataaaca agcaagtaaa acaaacaacg
      361 gaatcaacca ttttccataa gaaaaacgta taaaaaagaa acaatcaaaa atcattatca
      421 cactaactat ccaagcaaat agccaataaa aaaatccctt tagccaaaat tggcgaaaca
      481 tctttatgaa caaacgtact ggtgaaaaac tattttcttt tgctaattct tctgtcatac
      541 ctatcatcac gatgcaacct ggaccaataa aaagtaaaga aaaaataaag gaaaagacat
      601 tccttccatc tatcgacaaa aggaacgcag ctgctatata caagatattg acacaccaaa
      661 aacaaaggct acttgccaat gattgataca accaataaaa gaattccata taaacatttt
      721 tgtcaaaacg ctgctctttc ataatcacct cctattcctt cattcctgat gaagcgatcc
      781 cttctacaaa atatttttgc atcgctaaaa atataaaagc aacaggaaga acagaaacaa
      841 ctgaggcagc catgattaat gcgtactctg catcaaatag cccaataaac attttcaacc
      901 ctaactggat tgttttattt tccgtagaag acaaataaat gaatggcccc atataatcgt
      961 tccatgtatt aacgaatgtg atgatcgtca aactggcaat cgccggtttt gtaagaggta
     1021 aaatgatctt gcgatagatt ccccactctg ataagccatc gatccgcgca ctctcacata
     1081 aagaatctgg gatactactg taatattgct tcaacaagaa cacaccaaaa gcattgaacg
     1141 cttgtaatag caccaacgac cacaacgtat ctgttaaacc cacttttctc atcataatga
     1201 actgcgggat catataagac tgccaaggga cagcaatcgt gccgatatat gctaaaaata
     1261 aaatatcgcg ccctttaaaa tttagtttag aaaacccgta agcagcaaaa ctagaagtaa
     1321 acaactgaat caacgtgatc acaacgctta aaaaagctgt attcttaaaa aatgtcacta
     1381 aaggaatacg agaccaaata accagataat tctctaaatg ccaagtttta gggatccatt
     1441 gaatcggaat cgtgaacact tcattatttg ctttgaatga agcagaaacc atccataaaa
     1501 aaggaatgat cgtcaataaa gctaatacaa tcagcagaat catacccaac cattgaataa
     1561 agggcatgtt ttttgaacga atttttgctt tttttctttc atttcgaaca cctccaaagt
     1621 tataatcttc atttctttgt aatgattaca atccagaaca acggttatta tagctgattc
     1681 atttccgttc ttcttatttt tgtaagcgct tatttaacaa gttatgtaaa tctctttatt
     1741 tttttgattt aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat
     1801 tgaaatttct tcgattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat
     1861 caattcattg actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt
     1921 ttcaatcatt gggtcaagcg gaacgaatgt cactcgcttt gaatgatgac ttacagcacc
     1981 ttctatgact aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa
     2041 attgtacgtt cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat
     2101 tcgctgtatt tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt
     2161 tatcacgtct ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt
     2221 tcttggtaac aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa
     2281 atctaacagg cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg
     2341 aaattgaacg ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga
     2401 actatcagct tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt
     2461 ctcgaattct tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta
     2521 gaaaataacc tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc
     2581 ctagttactc ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa
     2641 cttctgcggc ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc
     2701 gtaactccat agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga
     2761 taacacacga tcatatttct tgatttttcc ttgtaaacgt tccaaacatt cttgcatttc
     2821 agcaattttt ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac
     2881 agttgcttct ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc
     2941 ggcatttcgc atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg
     3001 gattccgcct tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa
     3061 tgtatcggcc gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt
     3121 tctcttaatt tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac
     3181 tccaagtcaa tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg
     3241 tattatttca aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat
     3301 ttagggaaat tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattagt
     3361 atcgacatcg gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt
     3421 atcttcaatg attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat
     3481 cactaatttg gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg
     3541 acgtgtgaaa gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta
     3601 ttttgcttca aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg
     3661 aggagaatat ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg
     3721 acgagcttct tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt
     3781 agctgctgtg atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc
     3841 ggtgttgatg gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt
     3901 acaatctgcc gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg
     3961 gcaaacatgt ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat
     4021 gaattggcta ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt
     4081 gtggatggtc agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat
     4141 ggataaattg gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt
     4201 ctatctactt gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg
     4261 ctactttatc aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac
     4321 agaaatccgc gcgacggcga ataatttcgt tatgaacctt ggacgagctg ttggtggttt
     4381 ttcttctatc gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt
     4441 tttgagcggt atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc
     4501 attaagggaa agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg
     4561 aaaaagcttg catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc
     4621 gtttggctgg tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat
     4681 ctggcggcga ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat
     4741 aacgaaaaac tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct
     4801 caggatgttg atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa
     4861 gataaataat gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct
     4921 cattcttcaa aatgacctta tcaggatatt gttggaacaa ttcttctaaa tctgttagat
     4981 aattcaagat agacttactt gataccttca attcgttttg tagttcttct attgaacacc
     5041 aacgttctgt ttcaagcagt aaatgaatca ttttaatttt cttttgagaa actt
//
LOCUS       DACDKL010000165         5078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDKL010000165 DACDKL010000000
VERSION     DACDKL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.167"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(174..863)
                     /locus_tag="IV329_002268"
     CDS             complement(174..863)
                     /locus_tag="IV329_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8941431.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(908..1672)
                     /locus_tag="IV329_002269"
     CDS             complement(908..1672)
                     /locus_tag="IV329_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8941432.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1700..2398)
                     /locus_tag="IV329_002270"
     CDS             complement(1700..2398)
                     /locus_tag="IV329_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8941433.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2411..3190)
                     /locus_tag="IV329_002271"
     CDS             complement(2411..3190)
                     /locus_tag="IV329_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8941434.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(3206..4150)
                     /locus_tag="IV329_002272"
     CDS             complement(3206..4150)
                     /locus_tag="IV329_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8941435.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4191..4970)
                     /locus_tag="IV329_002273"
     CDS             complement(4191..4970)
                     /locus_tag="IV329_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941436.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         1303 a         1076 c          907 g         1792 t
ORIGIN      
        1 tcgaatattt tcttttatat aagaataatg atactgaatt gccccattca cctttctttt
       61 tttagatttt ctacaagtga ttcaaaccct ccataattac agttattact tttgaaacga
      121 taatatgaac atgtgtaatc ttttttatta acaatccatc attctatccc taactaatat
      181 gctccattgg gcttgacaat aacataaaac gtttttaaaa tcaacctcaa gtcgtacatt
      241 aaacttcgat gactaatata ctctaaatcc aactcaacca ttttatggaa actaacatta
      301 tttctatcac taacttgcca caaacctgaa caaccaggtt ttacatacaa gcgttgaaga
      361 tcatatctcg tgtactcagc tacttccctc tccaaaggtg gccgtggacc aattagactc
      421 atatcgcctt ttaaaacatt ccatagctgt ggcaattcat caatgctttt ggcacggata
      481 attttaccta ttttagtgac acggggatct tctttcatct tgaacattgc accttctatt
      541 tcattttgat caagtaattc ttccaatttt tcttcagcat ctaaacacat cgaacggaat
      601 ttatacattt tgaatgtttt tccattttta ccaacacgtg tatggcaaaa aaatatcgga
      661 cttcctggct cttcttttct gattcgataa gctacataga acatcaaagg agaagctaaa
      721 atcaaaccaa taatgcttcc tacaatatca atcattcttt taaaaaataa atagatatat
      781 cgtttttcaa ctttttcttt atccaaccaa acttgattag gatgtacttc actaatacga
      841 gataataaat cttcactttc catactttcc ctctctcctc atttcctttt tatctacgaa
      901 taaagaacta aaataaacca aacttcttct tttttacctc ttcatagact ggataactga
      961 tttcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac
     1021 caaagtcctt agcaatctgc tcatagcact cttttaagta aaaggtacgc ttagagaccc
     1081 catgggcatc agaagcagcc atctgcacaa gattatgttt gaccatcatt ttggctgttt
     1141 tctggatgtc ttttccaaat acaccgacat aacttggtgc ggtgagttgg gccaagcatc
     1201 ccatatctag gaatggcagc atatgattcg ggtctttacg aaaatgcgcg ttgcgttctg
     1261 gatggacgat cactggtgtc tttcctagtt gtaacaactc aaaaaagagc tgttctgcat
     1321 ataacggcac ttccagtgtc ggaaattcga ttagaagata tgtatcatct aaatctgtaa
     1381 ataaaatttc atctcggtcg atgtcttcga tcagtgttcc ggtaatacgt acttcttgtc
     1441 cttccaataa cgtcaaaggc aattgtcttt tctctaattc tgcttgtaac gaagcgacta
     1501 gagaaatgac ttgtgatttt tcattgctgt attttccatt attatgatgg ggtgtgcata
     1561 aaatatgtgt gatcccttgt tgaatcgctt tttcagccat ggcgatactg gcatctatgt
     1621 tttctgctcc gtcatcgatt cctggcagga tatggcaatg tagatcgatc atttctcttg
     1681 tctcctctct ttttatttct taattcccgt agtaatagcc agcatctttg ctgtgttctg
     1741 ccccattgta tacaacacca aggactcgcg cttgtaccat atccagcaat tcttttgctt
     1801 tagtcaatga ttctttccgt gacatatttt cacgtacgac caataaggtg ccatccgttt
     1861 tggaagacat gatctgtgca tctgttacgg caactactgg cggcatgtcg aagatcacta
     1921 catcaaatag ttgtcttgct tctgctaaaa tctgatccat tcttggcgaa cttagtagtt
     1981 ctgatggatt tggtggttta ggtccacttg gcagaatcga caaattctcg actggtgttc
     2041 gttggatctc atctgctaca cttcctgatg aactaagaac ggtacttaat ccgcttgcgt
     2101 tgttcaactt gaacgtttta tagatgacgg gcttacgtaa gtctgcatcg actagcagca
     2161 cccgttgacc agatttggca aaaacaacgg caatgtttgc tgcagtggtc gatttccctt
     2221 ctccaggtcc tgatgaagtc acaacaatcg ttttaatttg ctgaccagct gcggaggcaa
     2281 actggatatt cgtccgaatc gtacgatatt gttcagcgat tggtgaagag gggtttgtga
     2341 gtgtgaccaa actgactgca tgagtttggg ctgctttttg tttctgtgtt cgtgccattt
     2401 ttctttcctc ctagactctt gagcggcttc tgcgtgatgg cgctgtttta tcgtttgtag
     2461 cttttggttg tgtcggagct ggtccttttt tacggatacc agcattcaat tctttcgctg
     2521 acatttctgg tacagtccct aagatcggga agcccaatgt atccgtcaca tatttttcat
     2581 cttttactgt ccggtcgagt aattcaaata agaaagctag acctacgcct accatcattc
     2641 ctaacactaa gccaatcact agattcaatt tattatttgg cgaaacaggt gtcgtattgg
     2701 caactgcacc tgagatgatc gagattttat cgacattcat cacgtctttg gcgttctctt
     2761 ggaatacttg tgacgttgta ttagcgattt gttgagccgt cactgcattt gtgcttgttg
     2821 cttggatcga gaacatttgt gaattttgtg actgattgac ggagatggca tctttgattt
     2881 gtccagcagt catttttaca ttgtcttctg tttctaaacg atctttgact tcatttagta
     2941 ctagatcact gacgatcata tccttatacg tattgatcat ttgcaggttc gtattgacat
     3001 cattgacatt tgcagtatct gattgcggca gtgtcacgat caattgggct tgtgagctgt
     3061 attttggcgt caagatgaaa aacgtcacga tgcctgagat ggccaagcca gcaaacattg
     3121 agatgaggat tgttatcacg tgtttgcgta atgtttcaaa aatttctttc aagctgattg
     3181 tctcttccat tttttctcca ctttcctatt tattctctag attcatttga tcttttaatt
     3241 cttttttcac acgatcaagt tcttgatcgc tgactttttg gtatgagata ttgtcttgca
     3301 tgaagccttc tccttgcagc tgatcttgct tgatcgtacc aaatgcatcc cgataatcta
     3361 aagcaatctt tttcatttca tcaaagctca tgtctgtttt catattcgac tctagagcat
     3421 tcaaaacgga ttgatagttg cttacaccgt tcagactcaa cacttttttc gctacacctt
     3481 ctacgatctt tctttgacgt tcttgtctgc cgtagtcacc atttggatct tcataacgca
     3541 tacgagaata agacaatgcc tgatccccgt tcaatgtgat tttaccgatc gtaaaatcat
     3601 agccatcttg tgagaaggtg aggtcattat tgacttcgat accaccaact gcatcaacga
     3661 gttcttttag acctttcata ttgattgaaa catagtgatc gattgggatg tctacatatt
     3721 tttgtacggt atccatcgcc atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt
     3781 cttttgtgtc atgtccaacg atgtcgacat acgtatcacg aggaatgctg acaatcgttg
     3841 tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt atctgatctt ccttgatcga
     3901 cccgacctaa atctcccgta tcaatcccca tcaatagaac agagaaagat tcttgtttgt
     3961 ccagatcaac tggttttgtt cgttctcctt gggttcgttc cactgattcg taagtttttt
     4021 caatagaatg tgagagatct agatataatt ttgctcctac tcctacgatt gctaatacta
     4081 atacgacaag aattgctaaa ataattagaa atatcttttt ccctcttgat cttcttcttc
     4141 gttttgccat agtttcctcc gtttgctagc cttgcttgaa ttctcgctta ttaatagctc
     4201 gtattgctat agcccatttg ctgagcgact gtttctgctt ttgacgcgta tgcggctctt
     4261 attttttcat tccggatctg ttcgatgaga ctgagtgcgg tcaaatattg atcgtaagta
     4321 gcagtagctg ttacttgacc gtctttcagc atcgtatcga atagctgatc tagttgcgtg
     4381 acttgcgcga tctgagtatc agcgaatcct agatattgca taacaaccgt tttccaagga
     4441 ctatctttaa aaaagctcat attttcacgt acacgagcga catctgcact tgtcaccttt
     4501 tctttgatga cgacatcgtc tacagcttgc tgccagtttg atacattttt agtaaataaa
     4561 tcgttgacag ctgtctggat cttcagtttt gattctgcat cctctaactg cgttaagact
     4621 gctttctttt cttcttggat cgcagcagct tctttaggta attctgattc cttgatgtca
     4681 taatcatctg ccgaaagttt gatagaagat agtttatgtg acaaatttga aactgtttct
     4741 tctgttattt caggatttag gaaaattttt tcttttgtac tgtacagctt atctacttct
     4801 tttgataaag aggtcacggt gtctttttga ttggtgatgg cagattctgc gtaattgatc
     4861 cgttgatgat gttgccgctg attattgaaa tggtatccaa acaagcaaat cacaatagcg
     4921 cctgtgccta ccgccacagt agccaataac ttgacgaacg gcttttccat agattcactc
     4981 ctaaatattg atgatagata aataaagatg tgattcattc ccatcagaat gagcgctttt
     5041 tattttaaaa gcataattaa actaatatat tcatagaa
//
LOCUS       DACDKL010000166         4952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDKL010000166 DACDKL010000000
VERSION     DACDKL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.168"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            127..351
                     /gene="nrdH"
                     /locus_tag="IV329_002274"
     CDS             127..351
                     /gene="nrdH"
                     /locus_tag="IV329_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8941437.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            510..2669
                     /gene="nrdE"
                     /locus_tag="IV329_002275"
     CDS             510..2669
                     /gene="nrdE"
                     /locus_tag="IV329_002275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8941438.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2719..3684
                     /gene="nrdF"
                     /locus_tag="IV329_002276"
     CDS             2719..3684
                     /gene="nrdF"
                     /locus_tag="IV329_002276"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8941439.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3773..4912)
                     /locus_tag="IV329_002277"
     CDS             complement(3773..4912)
                     /locus_tag="IV329_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8941440.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
BASE COUNT         1701 a          918 c          886 g         1447 t
ORIGIN      
        1 aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt gttgtttgac
       61 ttttttacta agagttttat actatctatt atagataata agaatgtgtt agaggaggca
      121 ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa gatggtcaaa
      181 cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga acagcctgaa
      241 gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac ttctgatgca
      301 gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta acatgactaa
      361 aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt ttttgtcgca
      421 aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat caagcatcat
      481 caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt
      541 atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct ttgaataagg
      601 ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg atgacttttc
      661 tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa gcagaattca
      721 ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac gagcaacatt
      781 tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg ttgaaaacca
      841 acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat gcgctttact
      901 ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc catcaacgat
      961 atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt
     1021 cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca atcaactctg
     1081 ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat cttcgtgaag
     1141 ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat
     1201 tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga gctggcgtgg
     1261 tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg
     1321 cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct gataaatttt
     1381 atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt gtcgaaaaag
     1441 aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat ctagttgcaa
     1501 atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat
     1561 tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac cgcgagaatc
     1621 caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa gttcaaacgc
     1681 cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc tcatgtaact
     1741 taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta
     1801 cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc gtaccaccga
     1861 tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc ttacacactt
     1921 atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt acaaatattt
     1981 acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa
     2041 aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat ttcgaaaaat
     2101 acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt
     2161 ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa gatggactgt
     2221 atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc aacgatacga
     2281 gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag aaaatcggta
     2341 aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac acttctgctt
     2401 atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa cacgtcgatc
     2461 aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga
     2521 agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac gctttccaca
     2581 aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa gaaatcggaa
     2641 gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta ttcatatttt
     2701 aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg gaatgaaatt
     2761 gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg gctagataca
     2821 cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt ggaaaaagat
     2881 acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc tgaaagtggc
     2941 atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt gaacaatatt
     3001 caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac actgaatacg
     3061 aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct gcaaaaaaaa
     3121 gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa aattgccagc
     3181 gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta ttatttagga
     3241 aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga tgaatctgta
     3301 catggaacct acatcggtta caaattccaa ttaggattca acgagttacc tgaagcagaa
     3361 caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga aaacgaagaa
     3421 cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa gacattccta
     3481 cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt tccagatact
     3541 gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag caaccatgac
     3601 ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat gaaagacgac
     3661 gattatttga ttggattgga ataaggattt attttaattc aaaaattttt cgaaaaataa
     3721 aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat gcttattcac
     3781 ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc attcttctca
     3841 ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag acaggtatgc
     3901 aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc aagatgatcg
     3961 tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa tttccatcta
     4021 attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga gagataaaca
     4081 aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg ataatattcg
     4141 tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt ctcattgcca
     4201 aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata taggcagata
     4261 atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct ttcggaaaga
     4321 agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc aaaataaatg
     4381 gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt gagacacttg
     4441 cagataaact gactaatatg atattgaaca aattactaag agataaattt gctgatgcta
     4501 attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca tttaaccatt
     4561 tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc tgttccatta
     4621 actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc aaaaacgtca
     4681 ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg attggatcga
     4741 acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa agcattttca
     4801 atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg atcgtgagta
     4861 ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc atacatctcc
     4921 tccaaggtcc tttgatactt ctattctatc at
//
LOCUS       DACDKL010000167         4929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDKL010000167 DACDKL010000000
VERSION     DACDKL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.169"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(87..1136)
                     /gene="recA"
                     /locus_tag="IV329_002278"
     CDS             complement(87..1136)
                     /gene="recA"
                     /locus_tag="IV329_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8941441.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1245..2486)
                     /locus_tag="IV329_002279"
     CDS             complement(1245..2486)
                     /locus_tag="IV329_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8941442.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2726..3304)
                     /gene="pgsA"
                     /locus_tag="IV329_002280"
     CDS             complement(2726..3304)
                     /gene="pgsA"
                     /locus_tag="IV329_002280"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8941443.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3513..4397)
                     /locus_tag="IV329_002281"
     CDS             complement(3513..4397)
                     /locus_tag="IV329_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941444.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         1326 a         1123 c          891 g         1589 t
ORIGIN      
        1 ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct actcttcaga
       61 ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc ttcttgtcct
      121 ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct aactagagca
      181 gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt ttcccgtcct
      241 tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac aatgtctttt
      301 tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata catgacatct
      361 acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat ctttgtacgg
      421 ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc caaacggatc
      481 gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc aaacatcacg
      541 cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt gttgatcgaa
      601 ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac atgtgagtca
      661 cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc gactactacg
      721 atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg ttctcctgtg
      781 tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc atattgcgga
      841 tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg tacttctgca
      901 atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac ttcgatgata
      961 cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga gccacttgga
     1021 attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc tttaccatag
     1081 ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc tgccaatacg
     1141 ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc atatcttttt
     1201 tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt tcttaaaagc
     1261 tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc agcacgatct
     1321 ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc gatccatact
     1381 gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa agctacactg
     1441 taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca ttctttgctg
     1501 actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc ttttgtttgg
     1561 agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat gtcgcctaat
     1621 gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg cttgttttct
     1681 ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc ataaaaatat
     1741 tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt ccctgcctga
     1801 acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc atatggtgcg
     1861 atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag tcgcgattca
     1921 ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg aaaacgttcc
     1981 tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg cggaccggga
     2041 agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag ccctgtacga
     2101 tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt tttggtcatt
     2161 tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt attttgtatg
     2221 agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc tgtcggtcct
     2281 aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat caactcttct
     2341 aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc tagatcagct
     2401 agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa aagttctgtt
     2461 ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta tacgctgtat
     2521 ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct tgtcacagct
     2581 ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc acaatataca
     2641 taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta gacggcaggc
     2701 tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg ctgtttttga
     2761 tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc atgatttgat
     2821 ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag ataatagcaa
     2881 ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact tctccatgtt
     2941 cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg atcgctacta
     3001 cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata actagcattt
     3061 tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc gcacgggcaa
     3121 tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca cctactagct
     3181 gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa ggtaaaccta
     3241 caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta tttggtaaat
     3301 tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt ttccaccgat
     3361 attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa ttgatctttt
     3421 tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt ttatacctac
     3481 aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag agtaaggttc
     3541 acattttttg gactctgatt gttttcccct ggattaactg gtacggattc tccattcgct
     3601 ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc tgtttccggt
     3661 aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata ggcattgtct
     3721 actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac aggcttggta
     3781 gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc tattgccatt
     3841 ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt ggtctgcgtg
     3901 ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga agaagctgtg
     3961 tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac gacaacgaca
     4021 atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa gcgacttgga
     4081 tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg ctgcggacgt
     4141 tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa atggtctcca
     4201 tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa tttaccaggc
     4261 aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat ttttgtaatc
     4321 tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa tttttctccg
     4381 atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg aagcatctgc
     4441 tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca cagctccttt
     4501 tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa tatccaataa
     4561 atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg gcaggctcac
     4621 gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca aaaaccggca
     4681 aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa cgctgatttc
     4741 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag
     4801 ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
     4861 ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa taagccgaaa
     4921 tatcacaaa
//
LOCUS       DACDKL010000168         4795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDKL010000168 DACDKL010000000
VERSION     DACDKL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.170"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IV329_002282"
     CDS             complement(<1..258)
                     /locus_tag="IV329_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941445.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IV329_002283"
     CDS             complement(274..924)
                     /locus_tag="IV329_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941446.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IV329_002284"
     CDS             complement(935..2107)
                     /locus_tag="IV329_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8941447.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IV329_002285"
     CDS             complement(2126..2998)
                     /locus_tag="IV329_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8941448.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IV329_002286"
     CDS             complement(3020..3940)
                     /locus_tag="IV329_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941449.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IV329_002287"
     CDS             complement(3940..4209)
                     /locus_tag="IV329_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941450.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IV329_002288"
     CDS             complement(4206..4586)
                     /locus_tag="IV329_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8941451.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1254 a         1052 c          791 g         1698 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatg
//
LOCUS       DACDKL010000169         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDKL010000169 DACDKL010000000
VERSION     DACDKL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.171"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IV329_002289"
     CDS             564..989
                     /locus_tag="IV329_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941452.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IV329_002290"
     CDS             2141..3118
                     /locus_tag="IV329_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8941453.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IV329_002291"
     CDS             3128..3625
                     /locus_tag="IV329_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941454.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IV329_002292"
     CDS             4001..4282
                     /locus_tag="IV329_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941455.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IV329_002293"
     CDS             4284..4700
                     /locus_tag="IV329_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941456.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDKL010000170         4782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDKL010000170 DACDKL010000000
VERSION     DACDKL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.172"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..712)
                     /gene="trmB"
                     /locus_tag="IV329_002294"
     CDS             complement(50..712)
                     /gene="trmB"
                     /locus_tag="IV329_002294"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8941457.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(782..1561)
                     /locus_tag="IV329_002295"
     CDS             complement(782..1561)
                     /locus_tag="IV329_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8941458.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(1768..2979)
                     /locus_tag="IV329_002296"
     CDS             complement(1768..2979)
                     /locus_tag="IV329_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8941459.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(2972..3709)
                     /locus_tag="IV329_002297"
     CDS             complement(2972..3709)
                     /locus_tag="IV329_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8941460.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            3847..4281
                     /locus_tag="IV329_002298"
     CDS             3847..4281
                     /locus_tag="IV329_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8941461.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            4274..4618
                     /locus_tag="IV329_002299"
     CDS             4274..4618
                     /locus_tag="IV329_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8941462.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         1517 a          993 c          786 g         1486 t
ORIGIN      
        1 gagatttatt tatggatatt aatgttttta tgaaagcaga aagttagttt cacagacttt
       61 tctttttggt cttatcttga aattgagctt ctactcgata aatacgttga ccttttgaag
      121 aaaatttttc ttcatactcc gtcataatat tgccttcaaa ttgactttga tgtaaatcta
      181 accatacctg tttgaggatc atgccatatt gagaaaaact tgccagagaa tactcaaaca
      241 atccttgatt atctgttttg aaatggattt ctccatttgg acgaagaatc tgttcatcta
      301 cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg acgttttttt ggccatggat
      361 cggaaaaatt cagatagatt tgatctactt cgctgtcagc gaaatactct gtcaacgctg
      421 atccatccac atgtagaagt ttcaggttag gaagcggttg ttcgatcaac ttgtctagtg
      481 caatcgaaac aacactgacc tgcatttcta ctccaatata attgatttca ggatgggctt
      541 ttgccattcc agtgatgaac tggcctttcc ccataccaat ctcaatgtgg attggatgat
      601 cgttttcaaa caattcattc cattttccct tccacaatgt tggatcacta attacaaaat
      661 tcgggtgagc cgctaacatt tccgctgctc ccggacgatt tcggacacgc attatttatc
      721 tccattcctt tcctctaatt aaaaaggtct ggccaaatct ccgcagagtt tgggccaaac
      781 ctcagccact gaaaattcgt tttaattgta aaatttcttc gttcatccgt cgatgatctt
      841 cacgtttgca atatctggtg atttcttgga gcaaattcat ggccgcatac caatgaaatt
      901 tttcaagcat ttcgtcagtc gggcgtatgc cataactgat cagccactga ttccagctag
      961 acagtggcac ataatttcct aaaatcgttg caatatccac cgcagggtct gcaaacatga
     1021 ttgaatccca atcgactaga tataaataat ttttacatac taaccaatta cgatggttta
     1081 catctccgtg tactactgat aagttttcct tatctattga cgggatctgc tcacgaagaa
     1141 aacgatggac aatttgcaga aattgatttt tctggagttc ttccggtaat tctttttcat
     1201 atttttgcag catcttctcc ggcgtcatga cacgaccacc gatacgggtc agcatggttt
     1261 taagagaatt tgaatgatgt aaatggtaca aaacatcaat cacgtcgttt ctttgaccaa
     1321 tctcattagg agtcagcaag cgaccttcca gccactcttg tgctgtcaaa gtatcgccat
     1381 tccccgttcg ttttgtccaa acaagttttg gtgtaattcc ttcccttgaa agagcagcca
     1441 acatcggagt cgtgtttcgt ttaataaaga ctttgtcctc atcacgcatg cctatgtatg
     1501 tttttcctgt gtcgcctttg atgggacgta accgccactc ttggtctaac tgaaactcca
     1561 tcatgcattc tcccgtcctc ttactctttg ttttgcagta cttattattc tagtcgcctg
     1621 aacactacac gtcaagcaca tatacgttct ttttcattta attgccattc aaaaaaccag
     1681 cttttctcaa aaaaaataga acgaacagca ttcttactgt caacgctcta tttttcattc
     1741 tcatttttac cctacacagc tttgttttca gtctgacatc ttcttcaaac gagttggtac
     1801 atatagttta acaaagagca tagtaacaat caaataagcc gctagcggaa cccatcgctg
     1861 ctgcccatct aatacgactg cagcaatcaa accaaaaata accgacgcga taattaatac
     1921 ccatgtgagt agatactgaa tcgcctgctt cttctgattc ttatggattg gataaagctg
     1981 aacgagaacc atatagcgga actggttcgc taaaggtaac agttggaaac cgatcaaata
     2041 taaaaataag aaaccgataa tcagactaaa ataaaagtcg actactccgg caatcaataa
     2101 cccacctata attactaatc ggaaataaag accactaaaa tcagaacctc ttgccaatct
     2161 tcttgcaaac aagtaaagat aagtgttttt tgattctctt ttaatatgag tcaatatagg
     2221 atccaaatag cgtcttcttt tgatctttgc cgtaatttca ggaacatctg tgaataaatt
     2281 gataaattga tagatctgat gcaacctatg ttcttctaca tgcaccattt tctcccaatc
     2341 caatcctttt tcttgttgct tccacaaaaa ccagtaatat agacttgcct gtactaatgc
     2401 acctgctatt cctgcccata ttagccaata aaatgataga aacaaaaaga tcagagaaga
     2461 aaaaaaccag cctaatttcc aataacgaat aacttgttga tctagtcgaa acaattctgc
     2521 tctcttgatt tgcagatgac tgaatttcaa actccataat atgatcagat agacgaaaaa
     2581 catcgaaaag gattgctccg tactaaccac cactaatggc atggccaatc cgcatgccaa
     2641 tatcaaaaag atcaacggaa aaatgcaaga atagcgaaaa gctcgttcca aataggtttg
     2701 catttctttt tctttaggaa gtaaaaaaac aatatctgct gcctctgcaa aagtagccat
     2761 cttacccacg aataaacaga gcatccaaaa tacagagatt atgagccctc caattgcaaa
     2821 cggtggctgc agggttttca accaattaga ataatataat cccacaccgc caatcaagaa
     2881 aagacaaact aaaacaaaat gatcgttcaa tacataacgc atatatttca tcattttctt
     2941 ttgatgtctt gctaaacgtt tttgaaaaaa actactcatg tgcctgctcc tttgttaaag
     3001 caatatagat ttcgtccaaa gaagaatccg gcagctgaaa ttcttttctc aacatctcaa
     3061 gagaaccgtc tgctcgtatc tctccgttat gtaagaccac aaaacgatca caatattttt
     3121 ctgcagtagc taaaatatgc gtagacatca aaataccggc tccttctttt ttcatttcta
     3181 ccatcaattc aagcaatgca ttgatagcta gtggatccaa gcctaaaaat ggttcgtcga
     3241 tgatatataa agaaggctcg atcaaaaagg cgcataagat catcactttc tgtttcattc
     3301 cctttgaaaa attagctggg aaccagtcaa gacgattaga taaccgaaaa gtctccaaca
     3361 acttttctgc tcttttcatt gctatatcca taggtatatc gtaggccatc gcagtgactt
     3421 cgatatgttc tctcaatgtc aattcttcat ataaagaagg agtctctgga atatacccaa
     3481 tttgtttgcg atattcttcc gtatttttct ttaaggatag atgattgatt tctatcgttc
     3541 cttttgaagc ttccagcaga ccgataatgt gcttgatggt cgtacttttc cctgctccgt
     3601 tcagtccaat caaccctact atctctccag gctgtacatc gaaactgata tcttttaaca
     3661 cagggagatg tccgtacccc cctgtgacat gatcgataat taaactcatt ctttttcctc
     3721 cgccatttat ttttatttat tataccacga agaaaatgac ggtattttgt ttttttcctt
     3781 acaatagctt taacttatgg taaagttaag ctgaagtatt tattctttat agaaaggatg
     3841 ttttatatgg aaaactgtat tttttgcaaa atcatcaatc aagaaatccc aagctataaa
     3901 atatatgaag acgacaaggt ctatgctttt ttagacatct ctcaagcaac gaaaggacac
     3961 acattagttg tgcctaaacg tcatgtagca gatatttttg aatatgatcc cgaacttgca
     4021 ggagaggttt tttcgcgtgt gcctaagatt gctcaagcat tggaaaaagc ttttccagaa
     4081 atgaaaggtc ttaatatctt gaataacaac cgtgaactag cctatcaatc tgtttttcat
     4141 tcacatatcc atttagtccc tcgctacagc aaagaggatg atttttctat tcactttgtc
     4201 aatcatcaag atagttatgg atcggaagaa ctaaaagcta ttcaagaaac aatcgtaaag
     4261 caggtgtcct gcgatgatta aaaacttttt caaaggactg ttatttggag ctgctgctgg
     4321 cactgtcgga ggtctgttat tggcgcccag gagcgggaat gaaacaagaa agaaactgat
     4381 cgaagaattg gaagaagcaa caactctgac aaatgattta aataacagcc tgaatcattt
     4441 taaaagcgca ttgatcgaaa caaaagaaac agctgaatct gtgattcctg ctttccaaga
     4501 agcaatcaaa aaagatatcg aagattttaa attccaagcc gaaccacgta tcacacaaat
     4561 caatgaacaa gtagaaaaac taaaagctga tctgccagag tatcctgaac aaatataata
     4621 atgattattt tctcaacaga tttaactata taagtgagaa aagtttgcgt atttcctagg
     4681 aatgtgatga aattttgaat tttgtttgaa actaccacga caacgttttt aaaggaaatg
     4741 ggatatggta tagttaatat ataaaaataa taactaatta ca
//
LOCUS       DACDKL010000171         4737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDKL010000171 DACDKL010000000
VERSION     DACDKL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.173"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..306)
                     /locus_tag="IV329_002300"
     CDS             complement(<1..306)
                     /locus_tag="IV329_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8941463.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     "
     gene            complement(329..1729)
                     /locus_tag="IV329_002301"
     CDS             complement(329..1729)
                     /locus_tag="IV329_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8941464.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(1733..2143)
                     /locus_tag="IV329_002302"
     CDS             complement(1733..2143)
                     /locus_tag="IV329_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8941465.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2161..2652)
                     /locus_tag="IV329_002303"
     CDS             complement(2161..2652)
                     /locus_tag="IV329_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8941466.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2662..3453)
                     /locus_tag="IV329_002304"
     CDS             complement(2662..3453)
                     /locus_tag="IV329_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8941467.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3446..4219)
                     /locus_tag="IV329_002305"
     CDS             complement(3446..4219)
                     /locus_tag="IV329_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8941468.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4375..>4737)
                     /locus_tag="IV329_002306"
     CDS             complement(4375..>4737)
                     /locus_tag="IV329_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941469.1"
                     /translation="LNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKI
                     PSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIE
                     IPKGEYELIYTIFEQTIF"
BASE COUNT         1489 a          973 c          619 g         1656 t
ORIGIN      
        1 attttcccag aacggtaatt ccataatatt tttatctaat aattcatatc ctaaatcttg
       61 aattgaattg aatacctcat ctatgtcatc cgtatctaat gctagatgat caatggctcc
      121 tctacactca gtagttttat cttcccatat ttctattatc gttccattca actcgaaaaa
      181 gccgacttta cttcccacat tcatttcttc attaatcaga ctaaacccta aggattcata
      241 aaagattttt gaagctccaa aatcttttgt tggaattcca atatgttgta aaccatttaa
      301 tttcacttac atcatccctt caattgtttt aaaatccaca ttcataattt attgaatagt
      361 ctagtttatc taccttgatt gctccctccg taacagtcat acttaatgct gttccgactt
      421 tatcatacat tctagtactt aaagcaacat tatcaatata taaaacagct atcgtatcat
      481 caacaattaa ctttagttca tattctttgc ctatttccaa aaatagtggt cgttgcagcc
      541 ccttatcaaa catttgaggc caaggatagt tcggagtttt tccgaaagta agctgattat
      601 taatcaatga aatacgaaat tcgtatcctt cttgagtatc actatctaca aataatttta
      661 aagcaaactc ctttgttaac gatgtaatag tcaaagttgt ttcaaataga aaataatctt
      721 cgattttagg aacaattact ctttcttttt ttccaaattc tttctctaac ttaaaacttg
      781 aaaatggagt gatttcttta aaattctcaa ttattttttc aggcagttta acaccaagag
      841 ttttgtctgt cttttgaata atttgatgtg gaacaagtgc accgccccaa tcccagttat
      901 tttgatcgtt ctccccttct tttgttgcca cccagccagt tagaaaacgg ccagtttcat
      961 tagttccgac tgttcttgct gcataatagg cttttccgtc gaaagcttcg tctactggtg
     1021 atttccaatc tgaaaataat cctgctccga ctcgatattt agtcttttta tcttcacaat
     1081 actctgaaaa aagtaaatac cacgttccat caatttcaaa tatatcaggc atttcaatca
     1141 tgttaaattc attattagcc cagtagtctc ctttgaaatt ccaatctttt aaatcttcgg
     1201 attcaaaata gaccaatctc cccgtcggct ttttcttact tgctggtttt ctagctccta
     1261 gtatcaaaac gtattttcca tgatcttgat caaaaattgc aaaaggatcg cgccaatcat
     1321 tcttatcata tccttcttga ggaacaagtt ttgctgcttc gccatccttt ttccaatgaa
     1381 ttaaatcttt agaacttgca atcattaaca cttctgaagt ttttccatcc aacttagctt
     1441 tgcgattatg tccagtatac aaagctaaat actcattgat tccttggaca atacttccag
     1501 cgtaaatgta ttgatcaatg tcatcgttgc caccacgatg aattacttcc ccgtattctt
     1561 caaatttcac aaaatcagtg gttgtaacta gtgaccaccc aaaaggatca gttaaaggtt
     1621 taggatttct cgtatctcgt tgatgaaata gataaaaagt tccatcttta taaaaaggca
     1681 tgcaatcccc aaaccaaaaa tctttaggct tgtaaaaaat tttttgcatt tatcactcat
     1741 cctcttcgtt agtatgtctt tgcatatctg caagacacgc aattgtattc atatttctta
     1801 gattttcgat gatatcatcg cttttttttc cttgtaatct caaattgaca gcctcaatca
     1861 aaattcctaa attcatgcca ccaagaagtt gaaacccatt ttgaaatact tcacttcttt
     1921 tagcaaattc gttgaaaggt gttccaccaa tcaagtcagc aaataccata acttcttgat
     1981 tttttacttg agaaactatt ttttcaatgt tttcacggaa ttcttcaatg cctgcatctc
     2041 caagtgttac cacatgacaa tttaaattat cgccaaaaaa catttttaat gtatctacaa
     2101 atccaatggc aatatttcca tgagatgtaa ttataaactc catccactca tcctctgttc
     2161 ttaataaata aaatccttta aaaattcttt tgaatctcta ggagtttgta tcactgtcac
     2221 ttcaactcca tcactaatta gttctttaaa agcctctctc tcttcttcag taatatttat
     2281 cgtcggacgg atgcgttctg ttccattcct tcttgataaa tttcctacgt ttacacttgt
     2341 tattggtaaa ccgttatcaa tcaaaaattt aacatcgatt ggagaaactg caacaataag
     2401 aactttttgt ccttcatatt taccagaaag aatattttta actgctactt cccttgtaat
     2461 tattgaagtt ctaacattag gtggtgcagc gaggcgaact acagcttttt ttacgtcatc
     2521 attggctact tcattattaa taaccataat tctgttagca ctcaattgat ttgtccattg
     2581 tgttgctact tgtccatgaa tcattcgatc atcaatacga acatgtataa tacctttcat
     2641 tttttcatcc atattcaaca cctacttcaa aatatttaac gctcttaacg caatagaaac
     2701 tactaagatt aatagaatta cataaactgg tttaaccttt cgtcctaaca aataatacac
     2761 aattgctact acacatgcag gtaataaacc aggcataact tgatcaaaga tttcatttcc
     2821 ggtcattgtt aagtcgcctt gtttaaattc aaagccaaca ttcactttta caactgtggc
     2881 aatgagtgca ccgaccacca ttaaccctaa aattgatact gcatcagtaa tagactttaa
     2941 ttttgtttca atttgagtta gtaggctcgc acccgattct ttcccagcaa ggtataaagg
     3001 ataagcagta atccttaata aaattgatgc cagtatccac aaaataattc caacaaaact
     3061 accttccaat gccatattag ctgcaattgc tccaaaaata gtccatggaa taactacaaa
     3121 tacactatct ccaattcccg ctaaaggacc cattagacct gttttcagag cacttactgc
     3181 ttccaaagac ttattttttt cttctttttc aattccaaca ttcataccaa taatgtaagg
     3241 cgctgtatac ggcgtagtgt taaaaaattg taagtgagct aaaacggatt gtttcattcc
     3301 ttcaggatca tctttatatg tgtccttgat aaaaggaagc atagaatacg cataacccaa
     3361 cccttccatt ttttcataat ttaatgaagt tgcaccattg agaatccacc ggaaaattac
     3421 ttgtttgtcg atttttgtca gtttcttatt catcttcgtc aaaacctcct gttgcagctg
     3481 gtgctacttg agtattattt gatctatttt tatatacgat tagtgcgatt gcagcgccaa
     3541 gtaacgaaat ccctaaaatt ggtatttgga aataaactgc caaaacgaaa ccaactaaca
     3601 aataggcaaa gtaatctttt gtaggtaaat atttaagtag cattcctatc ccaacagcag
     3661 gtaatagtcc ccctgctacc tttaaaccac cggtaaccca ttcaggaata taattgattg
     3721 cacttcccac taacgttggg cctaatatca cagttaacaa aacaggaatt ccagatacaa
     3781 agcttgtatt aattgatgcc aaatattgca tccactctaa tttttgataa tcttcttttt
     3841 gtgcgaattt ttctgccttt tgttgaaaaa aaatgtttgt tgaccacttt agaacatcta
     3901 gttgtacaat taacattgct accggtattg ctaaagttat tcctatagaa gcatcctggt
     3961 tagttgagat tgctaaataa gtaccgatca atgctgctgt ttgataatcc ggtactgatg
     4021 caccaccaaa actagtaata cctagtgtca ttaattgcaa agtcccaccg atataaagtc
     4081 cagtttgaat atctcccata ataagtccgg tcaagaaacc tgcaacgaca ggttgggagg
     4141 ttccgatttt gggcccttcc ttatcccagt taatgaaaaa tccatacaac acaactaaaa
     4201 atgtttgcca aaacgccatt ttgttctctc ctttcttctt atacttttat ataagcaatt
     4261 actgtgccaa ctaacattat ccaataaagc gggaaaatac agtaaatatt aattaaatga
     4321 gcgtctaaat atactttgga caccctttca tttttttaga cgctagtatg ctatttagaa
     4381 aatagtctgc tcaaaaattg tatagattaa ctcatactct cctttgggta tttcaataca
     4441 gtaaatatct ttgatttctg aaaaaaaatc ttttgataca tttaaaaaag tatcaaatag
     4501 ttgcgctccc tctttcaaat gttcatttgt tattccatca ccaatcataa ttcgttcaat
     4561 cattccagaa agatgtagaa atagattaat tctcagaaaa cttgggattt tgacatcgta
     4621 atatttttca tacaaatata gtactttttc tatctcattg aatacttttt tggaatctaa
     4681 aaagcgaagc ttagtactag cgccttcaat agtaaagagt ttaactaatt catttaa
//
LOCUS       DACDKL010000172         4709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDKL010000172 DACDKL010000000
VERSION     DACDKL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.174"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..508
                     /locus_tag="IV329_002307"
     CDS             <1..508
                     /locus_tag="IV329_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8941470.1"
                     /translation="RQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLD
                     HSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEG
                     TLRYSLGY"
     gene            540..1262
                     /locus_tag="IV329_002308"
     CDS             540..1262
                     /locus_tag="IV329_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8941471.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            1320..3359
                     /locus_tag="IV329_002309"
     CDS             1320..3359
                     /locus_tag="IV329_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8941472.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIIAAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3468..4619)
                     /locus_tag="IV329_002310"
     CDS             complement(3468..4619)
                     /locus_tag="IV329_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8941473.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1713 a          872 c          905 g         1219 t
ORIGIN      
        1 tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa caaaaaaatc
       61 ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc cgattactta
      121 ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat cggaagaagc
      181 gaatcttccc aatatcctag gggacaaagg atatattagc cataaaatcc atgaaaagct
      241 tgcccttaag ggcattacta tatccgttcc gccacgtaaa aacatggata aatcagaaaa
      301 actagaccac agcttacttg gaaaacaaag aaaaacagtt gagactgttt tttcttcctt
      361 agaaaaatta ggttgtcaaa atttcaactc acgttctgtt aaagggctag agagtagatt
      421 tgaaagcata ttactggctt acagtgttct attaagtcga gcacaacgac gttttgaagg
      481 aactttgaga tattctttag gatattaaaa ttaactagca ccacgggtta ttatctattt
      541 tgttactgct tacattagta gttggctatt tttcgattga gtttatccat ggattttcgt
      601 ctgcaaaaca gagttcaact gtgaaaaagg tagagccgaa aagtatccct accacactaa
      661 atgtggcttt gattggttca gatgctcgtt cgaaagacga aaacggtcgc tcggattcac
      721 ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct atcatgagag
      781 attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct tactcttatg
      841 ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa gctccctatt
      901 atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt cctggtggag
      961 tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac aaaggagagc
     1021 aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac aaagaaggag
     1081 acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag cagctgaaag
     1141 acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt agtatccaaa
     1201 cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa gacatatgtt
     1261 agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa
     1321 tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga
     1381 tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa gaaacccaag
     1441 cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac aagggagatt
     1501 ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa
     1561 aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa ggacttcaga
     1621 tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca tataaagcaa
     1681 agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca ttagacagaa
     1741 atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa atggaaggaa
     1801 atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata gatcgaaatg
     1861 gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca agtaaacttg
     1921 gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc gacttaacag
     1981 aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac tttgtcccat
     2041 tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt caagaagtag
     2101 acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac gtgggagata
     2161 tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa atcggtcgct
     2221 caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg aaattaagca
     2281 ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt caaaatggca
     2341 aagatatcaa attgacaatc gatgcgaagg cacaaaaaac agcttttgac agtctaggag
     2401 gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta
     2461 gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa gattacaaag
     2521 cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt tatgctcctg
     2581 gatctacgtt taaaataatt gcagcagcaa tcggtctcga caacggcact atcgatccaa
     2641 atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg ggatcttttc
     2701 aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct ttgatctatt
     2761 ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag
     2821 gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg aatccagcac
     2881 aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact ggatatggac
     2941 agggcgaact tctaattaat cctatccagc aagcagcaat gtattctgtt tttaccaaca
     3001 atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat aagaagaatg
     3061 tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt gtgcaagatg
     3121 taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg aaaactggta
     3181 cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc ttgtttgctt
     3241 tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg
     3301 attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat tatcaataaa
     3361 aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc cttttttcaa
     3421 ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca cacttcataa
     3481 actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa taaaatcacg
     3541 ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa
     3601 atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa tgaactatgt
     3661 tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt caaaatcgct
     3721 acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa atcaacttct
     3781 aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc ctctacattg
     3841 tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa aaaagcacca
     3901 atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca aataactagc
     3961 gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag taggaaaggc
     4021 gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca
     4081 agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct gaaacttaga
     4141 actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga tccttctttt
     4201 gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga agttgccgca
     4261 agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac
     4321 cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc aggtcggaaa
     4381 tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa taagatcaca
     4441 ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa tccagcttgg
     4501 cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa tctccgcgag
     4561 atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat aaattccatt
     4621 ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat tattgaaaaa
     4681 ataggaaaat atactcacaa aaaaaccaa
//
LOCUS       DACDKL010000173         4661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDKL010000173 DACDKL010000000
VERSION     DACDKL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.175"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IV329_002311"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IV329_002311"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8941474.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="IV329_002312"
     CDS             2714..2938
                     /locus_tag="IV329_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941475.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="IV329_002313"
     CDS             2951..3451
                     /locus_tag="IV329_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8941476.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..4661
                     /locus_tag="IV329_002314"
                     /pseudo
     CDS             3548..4661
                     /locus_tag="IV329_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
BASE COUNT         1674 a          691 c          996 g         1300 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaaataatat gacatttctt agcttttctg ataacccaca ctcgaaagga aattcaaaac
     4261 atggactttg gggaataggc acaagatatt cctatataaa aatgaaggac gaacctgact
     4321 tgggatcact tcgcgatgct ttaatgctag gcgaaactcg agttcattct gatttttcta
     4381 atttctccat taatgaaaat gaagttttat tggaaaaatt aagcttttct gggaccactc
     4441 ttagcacaaa agaagttgta gtcgaattca gcgataattt aacttcaatt attggaggaa
     4501 gaggaacagg caaatcttgt attacaagat tcttggtcta tgtccttgga aaggaagcag
     4561 aacttgatca attttctgaa atacaaagtg attatcagaa ctttgcacaa attgaacata
     4621 atggctctgg aatatttttg aaggatacaa ttgtgaaact g
//
LOCUS       DACDKL010000174         4603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDKL010000174 DACDKL010000000
VERSION     DACDKL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.176"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..896)
                     /locus_tag="IV329_002315"
     CDS             complement(<1..896)
                     /locus_tag="IV329_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8941477.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKK"
     gene            complement(898..2337)
                     /locus_tag="IV329_002316"
     CDS             complement(898..2337)
                     /locus_tag="IV329_002316"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8941478.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2522..4468
                     /locus_tag="IV329_002317"
     CDS             2522..4468
                     /locus_tag="IV329_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8941479.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1470 a          782 c          757 g         1594 t
ORIGIN      
        1 atttttttca gaagaatatc aacaataaga cttgcaattt catcaattgg ttgtcttatt
       61 gttgtcaatt gtgggaaaaa tttctcaata aaagaggtac catcgtatcc aataattttt
      121 aaatcttctg ggatattaag tttaagttga tgagcaattt tcatagtgag aatggctgtt
      181 aaatcatcag aaacaaaaat accatctggt ttgtgattta taatagttga ctttatttcc
      241 atttcacgac ggatagtaga cagattatta ggaattttga aaatttttcc atttggattt
      301 tgaaaagaaa aacctagtgc acgtaattct gtgggagaat ccgtgttatc atttcctgta
      361 atcataatgg tattttgaca accatttttt tgtaaagtct tagctgcaag ttttccccct
      421 tcaaagttat ctgaagaaac aatggaaaca ttaggagcta aatttctatc aaaagcaaca
      481 ataggtgctc tcacacgctc ataatcagca attccaagat tatggcttga tgagattatg
      541 ccatcaactt ggttggcttc aagcatttca atgtattctc tctctttagc aggatcattt
      601 tgactattgc aaataatcgt tttatatcca tgtttaaata actctatttc gagatactca
      661 atgagttcag agtaaaagat attactaata ttaggaaaaa taagaccaat aagctgaggt
      721 gactttcctt gaagtccacg tgctaaatta tttggtttat aaccaagagt tttcatagct
      781 tcatgaactt tttgttttgt tttttctgat aaatatccct tattattaat gacgcgtgaa
      841 acggttgttg ggctaactcc agctaattca gcgacatcag ttaattttgc taccatttta
      901 atattttaat tcaaagtatt ttccagtagg ttcgcctgat ttgatttgaa taccattttg
      961 gtgattttca gggaatacac gactagtaaa cactttttct cctttattaa taaaaatctc
     1021 aatgattgag ttatctacaa aaatgttagc agttgtttct ttagtatcaa ttgagcattc
     1081 acgaatcgta ccaaagtcag tggcatattg aatgcctgct ttgctgcgat ctaaaataat
     1141 ttttccttct ttggtatcta caatcaagct aagtccattt cctttactat cagagaaaag
     1201 aagtaactcc gatttctgat ttggaggaaa agttagttca agctcataag tattatttgt
     1261 ttctgctttt gctgtaaatt gttcagaact agcacgtaat gatgtaatag cttctactgg
     1321 atattggtaa agtttaccat cttttattga aagttctttt acaagactca ttgctccctg
     1381 ataatcatac ttatcagttg gataatcgat atcaggtagt ccaatccaac tgacaatcag
     1441 agcacgacca tcaggagtat tgaatccttg agtcgcatac gcttcaaatc cataatcaag
     1501 attttgaatt tctgaagcgc ccacaagttt tactttttct atgtcaagcg attgacagat
     1561 cttaaaagta ttcggataga tattcccata atttagctca gatttatcca acccttgagg
     1621 acaataaatg agaacaggtt ttttgtctac aaatactaga tttggacact caatcatgta
     1681 ttctgaacca gtgccgccaa aatcaaggtt gccaacttcc tcccatgttt ccacgttatt
     1741 gtcatccgct ttgtaaagtt tgataaagcc tgatttttct aaattttgtg ctccaacaat
     1801 cgcatacaat tgtccttcat aattaaacat ttgtggatca cgaaaatgat cagttacatc
     1861 agcaggttta tgaataagaa cattctcacc tttcgtaatg ttgtagtctt tatccatcca
     1921 tgctccgatt tgaagtgtat cacgttccca attttcattt cgaacgtttc ctgtatagag
     1981 taagaataga ttcccctcta tttcatatgc agaaccggaa taagcgccgt ggctatctcg
     2041 ttttgtgtct ggaagaagtt ctgctcctgt tacatgaaaa tgaactaaat catctgattc
     2101 attgtgtacc cattctttca acccatgagc agctccaaat ggccaatttt gataaaaaag
     2161 cgtatatttt ccgttaaaaa aagaaaaacc attaggatcg tttaacaatc ctgttttagg
     2221 ttcgatatga tagctagcac gccaaggtga tcgttttaca ttatccttaa ttttatccat
     2281 ctcatcttta gtccaatcag aataagcacg atatcgtatt tctcgaggta agttcatatt
     2341 ttactccttc aatttatctt tttacaacta gtatggtaaa cgttttccgt tgaaaaatca
     2401 atcatttcat ttaaaaaaat aaactatatg acaaacgttt gacatatttt taagatatgc
     2461 taaaataaat ttgcatttca gtgaaccgtt ttcacgtaaa aataaaaaag gagtgattcg
     2521 aatgaattac agtaaagtag cttccgacgt aatacaagcg gtcggaaaag ataaccttat
     2581 tgcagctgca cactgcgcta cacgtcttcg tctcgtacta aaagatgaca cgaaagtgaa
     2641 tcaaaaagct ttagacgaaa atccagatgt gaaaagcaca tttaaaatta atggacaata
     2701 tcaagtgatt atcggtgcag gtgatgttaa tttcgtttat gacgaactta tcaaaaaaac
     2761 tggactttcg gaaatttcaa cagatgattt aaaacaaatt gctagtaaag ataaaaaatt
     2821 caaccccatt atggcattaa ttaaattatt atcagatatt tttgttccaa ttatacctgc
     2881 ccttgttgca ggtggtttat taatggcatt acaaaacttt ttaacagctg ctggactttt
     2941 tggaccaaaa tcaattgaag aaatgtatcc tgcgattgaa ggtatttcct caatgatcca
     3001 actgatgtcc gctgcaccat tcatgttctt accaatttta gtaggtattt cagctgctaa
     3061 acgttttgga gcaaaccaat ttcttggtgc tgcaattggt atgatcatga caacaccaga
     3121 tcttggtggc tcaactgaat attggaatat ttttggttat catgtttcac aaacaaacta
     3181 tgcttatcaa gttattccag ttttagcagc tgtttggata ttatctatat tagaaaaatt
     3241 ctttcataaa aaattgccat catctattga ctttacattc acgccattat tatcagtaat
     3301 gattactgga tttttgactt tcacagttat tggccctgta atgttattgt tgtctaatgg
     3361 aattacagac attattgttt ggctatataa cactactgga tttattggaa tgggtatttt
     3421 tggaggaaca tattcactta tagtcatgac tggtttacat caatcatttc cagctattga
     3481 aactcaatta ctttcagcgt ggacaaatgg tatcggacat ggagacttta tctttgtagt
     3541 tgcatctatg gcaaacgttg ctcaaggtgc tgcaacattt gccatttggt tcttaactaa
     3601 aaattctaag acaaaaagtc ttgcttcctc tgcgggtgtc tcagcacttc ttggaattac
     3661 agagcctgcc ttgttcggag ttaacttaaa ataccgcttt ccattctttt gtgctttaat
     3721 tggttcagga attgctacag cagtagcagg attacttaaa gtagttgctg tttcacttgg
     3781 ttcagcaggt ttcttgggat ttctttcaat taatgcaaca tctattccat tttacgtatt
     3841 gtgtgaattg attagctttg tagttgcttt tgcaattact tacttctacg gtaaaacaaa
     3901 agctgctggt atctttaaag cagaagaaat tgctgaacaa acaccagtta attcttcaaa
     3961 agtagcaaca acagatccaa tatctactga tgttgaatca acacaaacaa aagtttcatc
     4021 agaaacaatt gttagtccca tttctggtaa agtgattagt ctttcttcag taaatgatcc
     4081 agtcttttca tcagaagcta tgggaaaagg gattgccatt aaaccaaatg gaaacacggt
     4141 ccattctcca gtagatggta ctattcaact cacgtttgaa actggacatg cttatggtat
     4201 taaatcaaat aatggtgctg aagttctaat tcatattggt attgatactg tatcaatggg
     4261 aggaaaagga tttacacaaa aagttactac taatcaaaaa gttagaaaag gcgatattct
     4321 tggagcattt gaccgttcaa tgattgagaa agaaggtctt gatgatacaa caatgattat
     4381 tgtgaccaat acttcggatt attcagaagt aaatatagta gctgaaaata cagtcactga
     4441 gggtactgat ttacttgaaa tcaaataaat atatttccct gagtaaagat ggaattttct
     4501 ttccaaaatc aaataatata gattaatcaa atatttgaga ataacatttt atttaatctt
     4561 ttcatatatc aattgtatga aaaattatta gaattttaaa aaa
//
LOCUS       DACDKL010000175         4600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDKL010000175 DACDKL010000000
VERSION     DACDKL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.177"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..1100
                     /locus_tag="IV329_002318"
     CDS             126..1100
                     /locus_tag="IV329_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8941480.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1082..1543
                     /locus_tag="IV329_002319"
     CDS             1082..1543
                     /locus_tag="IV329_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8941481.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1593..2057
                     /locus_tag="IV329_002320"
     CDS             1593..2057
                     /locus_tag="IV329_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8941482.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2071..2820
                     /locus_tag="IV329_002321"
     CDS             2071..2820
                     /locus_tag="IV329_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8941483.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2820..3650
                     /locus_tag="IV329_002322"
     CDS             2820..3650
                     /locus_tag="IV329_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8941484.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3650..4297
                     /locus_tag="IV329_002323"
     CDS             3650..4297
                     /locus_tag="IV329_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8941485.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4395..>4600)
                     /locus_tag="IV329_002324"
     CDS             complement(4395..>4600)
                     /locus_tag="IV329_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8941486.1"
                     /translation="ELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISYV
                     IEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1586 a          596 c          863 g         1555 t
ORIGIN      
        1 tatataaaat tgttataaaa aaattttttt gaaaaatagt attgcaaaat aaagcaattt
       61 catagataat aggtattgta aagttatatg actttataat acttattaga gtgagggata
      121 aagaaatgag acaactacac aaagaacaga ttgaaacagc aatggcagcg ttaaaggaaa
      181 agacaattga tcatgtatat tttacagcat gtgggggttc acaagccgtt ttaatggctg
      241 gtcaatatct atttgataaa gaattaactg taccttgtca tgtttatact gctaacgaat
      301 ttaactatgc tataccaaaa ggattatctg aaaattcttt agttatcact tgttcccatt
      361 ctggcacaac accagaaact gtagaagcaa caaaaaaagc aagagaattt ggagcaatga
      421 ctatttgttt atctaatgaa gaaggatctc ctctatggga agcagctgaa tatcctgttc
      481 actatgattg gggtaaagat gttgatgcta gtgatttaaa taaaggaatt ctttatggtt
      541 tattatttaa tattttactt gttttagatg gctctaaaaa atgggaaact tgcttgaatg
      601 aatttgataa gttaaatgat cttgttattg ctgaaaagag caaatttgaa ataactgcta
      661 aagaatgggg taaaaaccaa aaacgagata aaattattta cacaattggt agcggtatca
      721 attacggaga aatttactcc acagcagctt gctggttcat ggaaatgcaa tggattaatt
      781 ctaatgctat tcattctggg gaatttttcc atggtccttt tgaaatcact gattatgatg
      841 taccattcat cttagtgaaa agtattggag atacaagatt tttagatcaa cgggtagaag
      901 acttcactaa aaaattcagt aataaattaa tggttttaga ccaagctgat tttgacttag
      961 aatctataga tagtgtagcg aaagaatata ttgcagctgt acttagtggc gttgttattc
     1021 gccaaatggt agaagcgatt gcttttgaaa gaggccattc cttagatgtt agacgctata
     1081 tgtggcaaat gaaatattaa ttaataggag gttgcaaaag atggcaaatc aattatttca
     1141 cattgtgatt acaacacatt ctaccctttg tgagggttat ttaaaggcaa caaaattaat
     1201 tttaatgaat gacgccacag gtattagtgc tgttccattt aaagaaaata ctagtccaga
     1261 tgattttgag gagagtatga aagaaataat tagtcagcat catttgcaac ctctaattat
     1321 tttaacggat ttaattggag gaacacctaa taatgtttct attaaacagt tgaacaacaa
     1381 tagaattcaa attattgctg ggattaattt acctctttta ctggagttat taatggccca
     1441 gtcagctggt aaatctatag aagaaataga ttttaaggaa ttactgaata actctcaaaa
     1501 aagtatggtt catattaatt cgcttttaaa agatgtcagt taaggtggca atttctattc
     1561 tataaatttt aaaaattgaa ggaggaaata gaatgattaa attagtgcgt gtagaccaca
     1621 gattacttca tggacaagta atattttctt ggactaaaca gtatgatatt aaccatatta
     1681 ttgtggctga taacaaagtt ccaggagatc caatgtctgt gatggctttg acaattgcta
     1741 aacctgctga ttgcaaatta gacattgtta aaattagtga tgttaaaggg attatggaaa
     1801 catccccaaa tgataattac atgattttaa ttaaaggacc taaggaagct tttgaattag
     1861 ttcaaaattt accagaaatt aaagaaatta attttggcgg tgtgtctaag aaaagcgagt
     1921 ccaaacaata tggaaaggca gtctttttaa ctcctgaaga attagaagcc acaaaaaaaa
     1981 ttcttgatca agaagtgaaa atatttgtgc aacaagtccc atcttcaagt attgaaaatg
     2041 tagattttag taagtaaggg gggaaaataa atgttattaa aagccatttt attaggttta
     2101 gttggtgtgg tagctgttat tgactccagg ttaattggtc gacaaaatat tggtagacca
     2161 ctaattttga gtacacttgc cggacttgtg ctaggtgact tgcatacagg gattgttctt
     2221 ggggcatctt tagaattaat ttctatggga ttcgtctcaa ttggggcggc tggacctcct
     2281 aatatgcaac ttggtagtat tattgccact gcttttgcaa ttttaactaa tagtagtaca
     2341 gaaacagcct taacaatagc tatcccagtg gctgtagctg gtgaatttat gagtattatc
     2401 atgcgcatga taattgctca atttggacat gctgcagata aagccattga aaataataaa
     2461 tttagaaaag ctcaaattat tcatatttat tggtcattta tttttaatgc ttttgtttac
     2521 tttattccaa tctttttaac agtatatttt ggtgctgacg ttgtggcaga tttagtagat
     2581 aaaattccag aaatgattac agacgccttg actgtctctg gaaatatgct ttccgcatta
     2641 gggtttgcaa tgctattaag cacaatgttg agtaaaaaat tatatcctta ctttattttt
     2701 ggattcttta ttgttgccta ttcaggtctt tcactaattg gcgtgacaat ttttgcagct
     2761 ctgattgcct ttgtaatgga tcaagttaaa tatggaaaaa gagaagaggc gcatggttaa
     2821 tggaaaatag tacagaaaaa aaacgtttaa tcacaaaaaa agatcttaat aagatgtgtc
     2881 ttcgttcatg gacaatcatg tgttcgtgga attatgaaag acaaatgcat atgggattta
     2941 tgtatgggat ggcgccaatt ttaaataaag tttataaaaa tgatgaagag aaaaaaagag
     3001 aagcttatac tcgtcatatg gcatttttta actgtacgcc tcaaatgact tcatttatta
     3061 tgggactttg tgcttctatg gaagaacaaa atgctcaagc agaagaagga aactttaata
     3121 ctgaatctat ctccatgatt aaaaccagtt taatgggtcc ttttgctgga attggtgatt
     3181 catttttcca aggtacttta agaattatta catttgggat tggattgtct tttgctaagc
     3241 aaggtagtat tttaggaccg attttagctg ttggcctttt tgctatacct tctattttag
     3301 ctgtttttta cggcggtaaa ttaggatatg agtctggcaa taaatacctt actaaattgt
     3361 atcaagaagg tcttatggat cgagtaatgc aatttgcttc tatcattggt ttggctgtag
     3421 taggtggaat ggtggctagc atggtgccaa ttgtcacccc tttaaaattc actacagggg
     3481 gaacagaact tgttttacaa gacttacttg attccattat tcctggatta ttaccacttg
     3541 tatgtacttt gggaatatat aaattgatta agaaaaatgt tagtacaaat atgctcttat
     3601 taggttgtat tttggcaggg tttgttttaa gtataatagg tatcttataa tgattaaggc
     3661 tctaattttt gatatggacg gagttttaat tgatagcgag attatgtatc aaaagtgggt
     3721 atttaatttt ttaaaacaag aaaatttaag ctatcctttg tattcttatc aaaaaaagat
     3781 tggaactaca gcaagtattt ttgatgatat agaaaagtac aatgagggat gtaacagttt
     3841 agaattgaag ggaaaattta ctaaatattg gaaatcgcaa aagattgatt acttggatat
     3901 ttttcctaat atactaattg aacagcttga atggttaaaa aagaaaaaaa taaaaatggc
     3961 aattgcttct tccagtccaa tggatactat aaatgaagtt gttaccactt gtaaaataga
     4021 gtcatatttt gattgtttaa ttagcggaag agatttgcca gaaagtaaac ctaatcctac
     4081 tatattcctt aaagcttcag aacagctcag aattccagtt gaagaatgtc tggtgataga
     4141 agattcttat aatggtataa aagctggtaa aagagcaaat atgaaggttc ttgcaattaa
     4201 agataaacgt ttttctcaag atgtaagtct agcagatgat gttatttacg atattaaata
     4261 tctgcgacaa gaagtacagg tgaaatttgg aatataaata agttttttga tgtatttaga
     4321 gaatattagt aaaaataaaa atcatgccca aatctaatca aaattgattg ctttgggtat
     4381 gatttctttt aatatcaatc tattacttta ggatctgaat catttttatg caccgtatct
     4441 aaagtgatat tttctgtgac ttcaataaca taactaattc tatcacaacg aactgtggat
     4501 tgagaataat gaaatggttg attattttta tctttgccaa ccttttctaa aataaatagc
     4561 ggatctccta ctatacattg caataagtcg gaaagctcag
//
LOCUS       DACDKL010000176         4488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDKL010000176 DACDKL010000000
VERSION     DACDKL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.178"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            192..1079
                     /gene="xerD"
                     /locus_tag="IV329_002325"
     CDS             192..1079
                     /gene="xerD"
                     /locus_tag="IV329_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8941487.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1098..1469
                     /locus_tag="IV329_002326"
     CDS             1098..1469
                     /locus_tag="IV329_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8941488.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1439..2236
                     /locus_tag="IV329_002327"
     CDS             1439..2236
                     /locus_tag="IV329_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8941489.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2220..2789
                     /gene="scpB"
                     /locus_tag="IV329_002328"
     CDS             2220..2789
                     /gene="scpB"
                     /locus_tag="IV329_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8941490.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            2795..3511
                     /locus_tag="IV329_002329"
     CDS             2795..3511
                     /locus_tag="IV329_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8941491.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      3593..3718
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3841..4452
                     /locus_tag="IV329_002330"
     CDS             3841..4452
                     /locus_tag="IV329_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8941492.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         1504 a          786 c          979 g         1219 t
ORIGIN      
        1 tttttaatac ctaaaatgaa aatggggctg aagaatcaat tctgattcat cagtcccata
       61 aattatagag ccgaaaaaag aaaccagtcc gtctgagtgt ctagacggct ttttctttgc
      121 tcagattatg gaaaaagact atcagaatga agcggtatct gatatgatag agaggcatga
      181 gggggattat tatgaaagag caaatcaacg attatctcca ctacttgaat attgaacggg
      241 gattgtccgc caatacacga caaagctatg aacgtgattt ggaacagtac ttgcattttc
      301 tacaggagca gaaaattgct tcatggcagg atgtcgatcg ctttttagtc atccgttttc
      361 tggagaagtt gcatgaagaa aaaaaagcga cagcaactgt tactcggatg atctcaagtc
      421 tcagaagatt ccatcagttt ttacggcaag agcgtatcac tgatcatgat ccgatgcaac
      481 atatcgatac gccgaagaaa gcacagaaat taccaagtac attgactctg acagaagtag
      541 aaaagctgat tgaagcacct gatacgacaa aaacgttagg gataagagat cgtgcaatct
      601 tggaagtcat gtatgctact gggatgcgcg tcagtgaatt gattggattg aaattgggag
      661 atcttcatct ttctcttgga ctggtacaga cagttggaaa aggagataaa gagcggatca
      721 ttcctttagg tgattatgcg atccaatggc tggaacgcta cttagacgaa gcacgtccat
      781 tacttgtaaa ggacgcatct gaaatgcatg tatttgtcaa taatcatggg aaaggtcttt
      841 ctcggcaagg aatctggaaa aatctgaagc agttagtacg agaggctggg atttacaaag
      901 aagtaacacc acatacgctg cggcatagct ttgccacaca tctgctagaa aatggagctg
      961 atttgcggac agtacaggaa ttgttaggtc acgcggatat ctctacgact cagatttata
     1021 cacatatcac gaagaaaaga atgacggatg tttataagca gcattttcca agggcttagt
     1081 cataggaagg tgagaagatg ttaacacatt accggaaaga aaaccgtaaa attgctatgg
     1141 gactattgag tttccataag aaactgacag gtaaaacttc tttgttgaag gagattgata
     1201 cgtacgaatc tgaagacgat tatgaacttt ttttctttac gccagaaggt tcaaccaaca
     1261 ttcaagggat tattggtatt tactgggcaa cgaacgatca gctgatcatc catgacattt
     1321 ctttgaatcc gtcttatcgc ggtgagaata tgggctttca aatgttagat gaattacagg
     1381 aaaagtaccc ggatgtctcc atccgtgcaa ctgaaatcac agagccttac attggaaaat
     1441 ggctagcaaa gaaaaagaag aaagagtgaa tgagttgacg gagatcaata ttaaattaga
     1501 tgtattcgag ggtcctctcg atctgctgct tcatctgatt cagcaaatgg aaatcgatat
     1561 atatgatata ccgatcgcta cgatcacgga gcaatatatg aattatatcc atacaatgaa
     1621 aacactcgag ctagctgttg caggagaata cttagtgatg gctgctacat tgatggcaat
     1681 caaaagcaag acattgcttc ctgtccaaga attcggcaca gacgatgaag aattatggga
     1741 agaagatcct cgagaagaat tggtcactca gcttttagaa taccgtaaat ataaatatgc
     1801 agctgaaaga ctgacagaaa aagcagagga acgaagtctt tattacacaa aagaacctat
     1861 gaacatcgat gggttagcgg atgctgaaaa gccgttgaag cgaggacaag tcaaaaaaat
     1921 cgatttgatc cgtgccatgc agaaaatctt agaacgtaag aaattttctc aaaaagtgga
     1981 aaaaacgatc acgcccgatg acacaacgat cgaagaacga atggtattta tcgaagagcg
     2041 tataaaaaga aatgcgcatg gctgtacttt cgattctttt tttgagtcac cttctaaaag
     2101 tgaagttgtc actactttta tggctttgct agaattgatg aaaaaagggc agatcattgc
     2161 gcaacaaaaa gaaaactatg aatcaattat gctttatcca gcagtaggag ataccgaaca
     2221 tgacgaaatt aagtgaactt gaagcccttc tttttattgt tggcgaggaa gggatcagta
     2281 atgaagaatt aagcggactg ttaaatctca gcgaagcaga agtcacaagt ttagtgaaag
     2341 aattaaaaga atcttacact caaaactcgc actcagcttt gcatattttt gaaacggaaa
     2401 gccattatgt attaacgact aaaaaagagc tggctccctt acttaagcga tatgcgcaag
     2461 ggacatcaaa cacattatct caagccgccg tcgaaacctt agcaatcatc gcttataaac
     2521 agccaatcac aagagctgaa atcgagcaga ttcgaggagt tcaggtttca ggagcggtac
     2581 agcgtctagc tgcccataaa ctgattgaag aaaaaggtag agtagaagga ccaggtcggc
     2641 cgatattata tggaacaact ggctacttct tagattattt tggtttgaaa tcattagaag
     2701 aactgcctga tacacaagaa atggaaattt cacttgaaga agacgtgtca ccagatctat
     2761 tctttgatgg aatgaataaa ggagaataat tgtaatggag agattgcaga aagtaattgc
     2821 ccacgctggc atcacttctc gtcgaaaagc agaagaattc atccttgcag gacgtgtaaa
     2881 agtaaacgga caagttgtaa gggaattagg gataaaagtt gggaaacatg acactgtgga
     2941 agtggatgaa gtcccaattt atcaagaaga atacggttac tatttattgt acaaacctaa
     3001 aggagttatt tctgctgttg cagatgacaa aggaagaaaa gttgttacgg atcttttgcc
     3061 gatggtaaaa gaacgtatct atcctgtagg tcgactggat tacgatactt cggggatctt
     3121 gctactgaca aatgatggag attttgctca gcgcctgaca catccaaaac atgaagtgga
     3181 taaagtttac gttgcaaagg taaaagggat cgctacaaaa tcgatgcttg cacctttgac
     3241 aaaaggaata aagatcgaag gacgaaaaac atctccagct aaattccaaa ttttatcagt
     3301 ggatcctaag acaaaccaca gtatcgtgga attgacgatc cacgaaggac gtaatcatca
     3361 agtgaaaaat atgcttcaag cagttggtct tcctgtgcaa aaactaaaac gtgaaaaata
     3421 cggagatctg actttacaag gcctacgtcc tggggattac cgtgaactga acaaaaaaga
     3481 agtcagcagt ttgctgaacc actcaaaatg aacacttact ttttataatt gttagatgtt
     3541 gcgttttttg aatagatatg tataatgaat atgtaaacaa aaattatata aggttcgtct
     3601 tcaggggcag ggtgaaattc ccgaccggtg gttatagtcc acgacctatt ttgcaaaaaa
     3661 atagctgaat cggtgaaatt ccgataccga cagtatagtc tggataaaga agatagagct
     3721 tatttgacga cgcttgatca aataactcta actctatttt ccctggaaaa tagagttttt
     3781 tcatttggag agaaacgtca aaaagttcgc tgaagaggag tcctaacagg aggatttcaa
     3841 atgaagaaca cccgtgtaca gaaaatggtt gcagtagcga tgtttgctgc gatcgcatta
     3901 gttttacagt attttgcttt tccgatcatg ccggctttta gttttttgaa gatcgatttt
     3961 agtgatatct cggtattgat cagcatgttc ttattcggtc cagcggccgg agttgcaaca
     4021 gcgttcttgc gttcagtatt acatctgctg actacaggtt tttctccgga taatttggta
     4081 ggagacactg ctagtttctt atcaacaacg atctttacat tcccgattta ttatttcttt
     4141 agtgtaaaga aacagcataa aggaagaaac aaatttttag gtgtcattac aggaaccatc
     4201 gctctaacta tttttatgag tatcgcaaat tatttcgtta ttacgcctct ttacttaatg
     4261 tttttagggc tgaacgcaac acagatgctg ggcatgtctt tagcaaacta tgtactgatt
     4321 gggatcgttc ctttcaactt gatcaaagga ttcatcgtga gtgctgtctt tttagtgctg
     4381 catgcgcaac ttcttccatg gttaagtcga aaacaacatc agttcgaaca acgtcataca
     4441 gcagtcaaat aaatatcaaa aagatcaaga aatttcttgg tctttttt
//
LOCUS       DACDKL010000177         4470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDKL010000177 DACDKL010000000
VERSION     DACDKL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.179"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            318..1049
                     /locus_tag="IV329_002331"
     CDS             318..1049
                     /locus_tag="IV329_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8941493.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="IV329_002332"
     CDS             1194..3221
                     /locus_tag="IV329_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8941494.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="IV329_002333"
     CDS             3329..4426
                     /locus_tag="IV329_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8941495.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          923 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt
//
LOCUS       DACDKL010000178         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDKL010000178 DACDKL010000000
VERSION     DACDKL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.180"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV329_002334"
     CDS             574..1008
                     /locus_tag="IV329_002334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941496.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IV329_002335"
     CDS             2298..3221
                     /locus_tag="IV329_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8941497.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IV329_002336"
     CDS             3237..3776
                     /locus_tag="IV329_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8941498.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDKL010000179         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDKL010000179 DACDKL010000000
VERSION     DACDKL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.181"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            98..373
                     /gene="rpsP"
                     /locus_tag="IV329_002337"
     CDS             98..373
                     /gene="rpsP"
                     /locus_tag="IV329_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8941499.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            385..636
                     /locus_tag="IV329_002338"
     CDS             385..636
                     /locus_tag="IV329_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8941500.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            761..1552
                     /locus_tag="IV329_002339"
     CDS             761..1552
                     /locus_tag="IV329_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941501.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1722..2243
                     /gene="rimM"
                     /locus_tag="IV329_002340"
     CDS             1722..2243
                     /gene="rimM"
                     /locus_tag="IV329_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8941502.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2233..2994
                     /gene="trmD"
                     /locus_tag="IV329_002341"
     CDS             2233..2994
                     /gene="trmD"
                     /locus_tag="IV329_002341"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8941503.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3130..3477
                     /gene="rplS"
                     /locus_tag="IV329_002342"
     CDS             3130..3477
                     /gene="rplS"
                     /locus_tag="IV329_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8941504.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3609..4370)
                     /locus_tag="IV329_002343"
     CDS             complement(3609..4370)
                     /locus_tag="IV329_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8941505.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDY
                     KRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         1485 a          784 c          871 g         1321 t
ORIGIN      
        1 gaatttaatc aagctgtaaa gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta
       61 catcttgtga ataaaacaat ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa
      121 acgtatgggt tctaaaaaga gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc
      181 tcgtgatgga cgtttcatcg aaactgtagg tacttacaat cctttgaaag accctgcaga
      241 agtagtttta aaagaagact tagttcttga ctggttatca aaaggtgcac aaccttctga
      301 cactgtacgt aacatccttt caaaagaagg cgttatgcaa aaacaccacg aagctaaatt
      361 ctcaaagaaa taaggtgacc ggatatgaaa gatttaagcg acttagtctt aacaatcgtt
      421 cgtccgctag tcacttatcc tgatcaagtc aagttagacg tcgtagagtc gaaagacttt
      481 tacgagtaca atctaacagt ggctcctgaa gatattggcc gtatcattgg aaaacaaggg
      541 cgtgttgcta aagcaatccg cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa
      601 gtgcgtttga atatcatcga tgataaggaa aagtaaataa acgtgctgta ctcgaatttt
      661 cgagtgcagc ttttttgttt tttttattaa ttgaagaaat gattatttgg caaaacaagc
      721 aagaaagcgt atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt
      781 tattccattt gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac
      841 acatctatca agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac
      901 ggcttccacc actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga
      961 ccagttgaaa gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg
     1021 ctatctcaat atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac
     1081 agatcgtccc ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta
     1141 tctatatcaa tatgggtttg cttctagtca agaaacaatc caagttttgc agccatttga
     1201 gatcgacaca aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg
     1261 tgcagcaggt tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg
     1321 gaataatata gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga
     1381 agaaaacagt ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac
     1441 gacgaatcga gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca
     1501 tcaagatcct atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg
     1561 cgctgatctg agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc
     1621 gcatttttgc ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac
     1681 atgctaaaat aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg
     1741 taggaaaaat cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg
     1801 atttcccaga agaacgttac aaaaaaggaa cggtgctaac actattccaa gacggaaaag
     1861 cacctgtgga attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt
     1921 ttgaaggaca tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt
     1981 caaaagacaa ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc
     2041 tgcatgtatt agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg
     2101 ccaatgatgt ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg
     2161 cttctgtcgt caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag
     2221 gactgattga cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct
     2281 atgggggaat ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct
     2341 aacttccgcg attattcaga taacaaacat cagacagtag atgattatcc atacggcggt
     2401 ggagcaggta tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa
     2461 gctgcaccgg atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat
     2521 cagaaaatgg cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat
     2581 gagggatatg atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat
     2641 gtccttactg ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg
     2701 ccagatgtgt tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg
     2761 gaacatcccc aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg
     2821 acaaatggaa atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc
     2881 tatcaacgtc ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt
     2941 ttagaagaaa tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt
     3001 ctaaaatctt ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc
     3061 acttaactat tacgatattc cgctgtgaga aaagctcata agaatatttg gaataaggag
     3121 aagaaaacca tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc
     3181 ccagcgttcc gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt
     3241 gaacgtatcc agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa
     3301 acttatacag tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat
     3361 acaccacgtg ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg
     3421 tactacctac gtgcattaca cggaaaagct gctcgtatca aagaaatccg tcgttaatca
     3481 tcgttatcag actgagaaag aacccttggt attcaagggt tctttttttg ttctctgttt
     3541 ttttatatta acgatttcta tttgggacat ttttgattaa taaaaagggg caaaaaaggg
     3601 gcaacaagtt acagtttttc tatatcttct ttcaattttt cttttactcc ctttgtcaca
     3661 tgcaaataaa tcttttcagt gatatcacta ttttcgtgcc caacgcgatc ctgaatagca
     3721 tacagaggag ttcctagttc cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa
     3781 ctaagttttt tatcaatctt catgtcagct ttatgatttc tcaaataggt gttaattgct
     3841 gttagttgga aaggagttcc tttcgttgtt tgaaatagaa attgaccatt cggatttaat
     3901 tctagaagct cattgtaaat agctatcgct ttttttggta aatcaatttc tcgcattcca
     3961 gcagcagttt ttgtagaatc agattttttc atatcagcta ttgaacgttc tctatacatc
     4021 atcgtcccat ttataactac tgatgcatta ttattggtaa tgtgtacatc gtctttactt
     4081 aatgcgattg cttctccagg cctcattcca gtcaaatata gccactggaa aaggagagaa
     4141 tatcttttat tgtgtgaagt tgtgaaattt actagtcttt tatattcatc atcttctaaa
     4201 aacttatctt taatttttat cgtttttgac tctcgtttat aatcaataac tacttcgttg
     4261 ataggatttt tttctacata gccttttttc atagcgtatg aaaaaagaag attcaatttg
     4321 gatttaatta cactgacgta cttatttgac aagtcatctt tatatatcat atcttcaaaa
     4381 atattattta aatctactgt attaatacca gaaacgatgt aagtctcagg tatctttttc
     4441 tttatagtgt ttaagatatt a
//
LOCUS       DACDKL010000180         4460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDKL010000180 DACDKL010000000
VERSION     DACDKL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.182"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1669)
                     /locus_tag="IV329_002344"
     CDS             complement(26..1669)
                     /locus_tag="IV329_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8941506.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            complement(1666..2025)
                     /locus_tag="IV329_002345"
     CDS             complement(1666..2025)
                     /locus_tag="IV329_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941507.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            complement(2186..2446)
                     /locus_tag="IV329_002346"
     CDS             complement(2186..2446)
                     /locus_tag="IV329_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941508.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2437..2748)
                     /locus_tag="IV329_002347"
     CDS             complement(2437..2748)
                     /locus_tag="IV329_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8941509.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(2749..3321)
                     /locus_tag="IV329_002348"
     CDS             complement(2749..3321)
                     /locus_tag="IV329_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941510.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3386..3613)
                     /locus_tag="IV329_002349"
     CDS             complement(3386..3613)
                     /locus_tag="IV329_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941511.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            complement(3654..3914)
                     /locus_tag="IV329_002350"
     CDS             complement(3654..3914)
                     /locus_tag="IV329_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941512.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            complement(3904..4092)
                     /locus_tag="IV329_002351"
     CDS             complement(3904..4092)
                     /locus_tag="IV329_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941513.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
BASE COUNT         1306 a          955 c          635 g         1564 t
ORIGIN      
        1 tacccctttc aataaggagt ataccttaaa attcaatctc atccaacata tcaaatgctt
       61 cctcataatc catatcagtt atttcttctc gtttatataa agctgccaaa aaagcatgaa
      121 acccatctgt tttacgcctt actggctctt ttttcaaaaa tgttttattc cctaatttgt
      181 cttctttgat aaacgaatta tttgtatacc aacgcatcat aggatcatca ccaaaaataa
      241 accgttcatt tgcaaaacca tcttcaataa taggagcaac ttttgactgc acacctctta
      301 tatttctgat aaactcaaaa ttatatgatt ctttctctag aagaggtttc agcaaatcca
      361 tacgaaatcc gtcggcacaa acgatttcta tttgatggat ttttcttttt tcatttaacc
      421 aatcaattaa atgacgagga tcaattgatg gctcatcaac gattgtcact aaaccttttt
      481 tctcccaatc cttaataggc gctttcagtt tgaatgtatc tagaaacttt ttcctagcaa
      541 aactatgttg ctgccagata aatttatcat caattttaaa aagcaaaccc actgttgcaa
      601 aatcacgaat acttgcataa tcaaatccag ccacacaaga acgaccattc aaatttggca
      661 attcttgttt agtagccatt aatttttcat gagtcgtaat atctgattct gcatcacctt
      721 ctgtaaaatt cattcgttta acaacaaatt cacgcctacc tgatggctca tcttctagtt
      781 ttttatattc tttcatcact gtagaaaaca gacgttttga atatgctgaa tcttcttcga
      841 acattggatt agcctttgac cacattttag gattatccat ttcatcaatg ttatccagtt
      901 tacaaataaa gggaaacaag cgatcattct tgttctctcc actaaaaatc ttttgtgctc
      961 gttccaacat tttgtcataa aaaccttctc taacatgacc atttgtacca ttataaaaag
     1021 ttcgaggatg ttttattttc cctagcccac tacgctgaat atcaactagg tctgaagatt
     1081 caaactgatg gatttcatca aattctaagc agccatctct tgcactatcc atagtcttag
     1141 gattgtttgt cctataacta aaaatcgaat tagttacatt gttgactata gccatcttag
     1201 tcaaataaaa ttgtttttcc agtttgcgca tttgtaccat ttcataaact tctttaaaag
     1261 atacctttcc ctgcttctca gaattcgctg taatcgttac atcataattt ctgacaccgt
     1321 agagagggct aataaaaaat gagtctcttg ctgacatgaa cccatttttc ccgccgcctc
     1381 gcgcaatact atttagaatt tcatcaaaaa acacctcgtc gtcctctttt tgatagcaga
     1441 aaatgaaagg tgtgataaat ttttgatatt tagctaaagg aaaaaaattt ttttcagtgt
     1501 acttgataaa cttttgaatc atttcttcat caaaatagat atcatcccga actagcactt
     1561 cgttttttaa ataagtaatt aactgaattc tttctttatt aaaagggatt accccttttt
     1621 cgtataaatc tacgtattca tcaaaataga atggctttaa tgttgtcaca gcaaatcact
     1681 accatctagc gctgcatttt ttgtaggttc gcgcttttct tctggcaaat agtcagtaag
     1741 ttgcttaatt acacgttgat atgcttggtc tctcgaatta tactgtttag caactggtcg
     1801 ttcccgctcg taaggttttt gattttctga ctgagagaaa agctcataat cacctttttc
     1861 ggaaatatca atccacattt catcaagaag aatttttaag cgtgccgctt gcgtaatcaa
     1921 accagaaacg accttctttt tatccttatc aatatcctta aacaatttat ttagacgcct
     1981 ttcttcagca gaaacaagcc tatttctctc ttgcagatca gccatcaact cacttccttt
     2041 ctacgtgggg agggggtcgt gtgaaaatag catcatttat ttgcagagtt gaccccatcc
     2101 accggttccc aaaaatttta tttcttctca aaatcttttg accgggggta tttcactcta
     2161 tatatcttcg ctttatactg atttatcagc cgtcttgctc gttgctttct acaatatgtg
     2221 caaatggact caaggtataa tcaatgtcta cattaatatt aaatgtccct gttacttcat
     2281 tagcgttacc tattagtcta ttgttgatag atatatcctc tacagttgtt gttccttcaa
     2341 tgagctgact cttaaataga accaacttat caatagcctt atcaaccata gcgattgctt
     2401 ctgctcgttt gacatcatct ctgtttgctg tttttcttac caccattcat catcccactt
     2461 tcttttcttt tgttggccac gatatttcat acggttgtgc cttttgttat ggcaatcttt
     2521 gcataaggtt cttaggttgt ttatatctag agctaaatcc ggatgatact ccagctcctt
     2581 tatatgatcc acttcaagaa ttgaatcatg ttgagttgtt agtctaccct ctgctttaca
     2641 ccattggcac tcataatgat ctcgctctaa gcattgactt ctaagtttcc tccactcagt
     2701 tgatccatag aacctagctc ttgcttgttt acttgaaaca tcaatcattt acatatctct
     2761 cttgtgccca atgatttttc tatctaagta cttgttatta tgtgaatcat aatattcaat
     2821 agtgatgtcg ttagctcctt ttggtacttg gcattcatat tcttccgtat cccaatgata
     2881 agtaatatcg acaagaccta tcggcatctc atccattcgt tgacctttat aataaacttc
     2941 tggtactgag tcagtatctc ttagtttgat ttctagaagg ttcgtattac ttccatcatc
     3001 taacgtcaat tcagcaatcc tctttttgat ctgttgaatt acttttcttc ctgtctcacc
     3061 tgtcattggt actaccgtct catatccagt aaactcgcaa gtaaagcgtt caatagcata
     3121 agcgttttga agtggcttag cactaagaac agctggatta ttgctataaa aacgatctac
     3181 taataacttc cctataggaa tggcatctaa agctctatca gtaaacacta aagcattggg
     3241 ataatcttct tgaattttgt aagctagatt gggtgcagtt acaacgttat acccttctct
     3301 gatataatct tttaatttca tagttttcct cctaaaaaca tctaataatt acttgctaac
     3361 aacttgatgt aatcatagtt atcattcatt tgatgtacct ctcaatgttg tattggatat
     3421 actcgtcttt ccaatagcca tgaccgcaat atatcagctt gcatttatcc acttcgtttg
     3481 gtgtagcttc tcttagcatt tcgacaatag agtacttccc tttgatttgt acagaacgca
     3541 caacacgcac tgaacaatca ccaatggttc gaggatattc attagttagt gatacatacc
     3601 agtagtttct cattatgcag cctcctttat gtaaagaaaa agcctagcac ctgctagact
     3661 ttgatacatt catttgattt tttgagataa gtatctacat agatttcacc tttgtccccg
     3721 ttcaacgtaa tttcaaaata cggtgctcct tttaagctag cgctgactaa tgctttgcta
     3781 ttttgcagtg ttttgcatga ccagactatg aaaatactaa actcaggcac tgctccactt
     3841 aggtcagcaa tctcattttc aattctttca cgtacaatag ccttgcattt ttcgataaac
     3901 ttctcatgat tcattttgta cctccgcaag cgcataacca gctgttgatg tattgttaaa
     3961 cgtagctgaa cgagtaactc ccgtagcctt cccagtataa ataaaactaa atcctgttgt
     4021 tgttggctta aaattctcca cttcataaaa atgatatgtt tgtccgttat tagtaaatac
     4081 aatcaattcc atttttcaaa atcctctctt ttttaaaata aaaagaccac tcaaagagtg
     4141 atctaatatg taatgcacag gcagggacgt ttccgatcct gtgcttgagt catttgacga
     4201 ttcatttgta ccgaatccca aaactcaatc taacccaacc aattatgtaa tagcaaccta
     4261 caagatgcac aaaacgcgta cgaaattgcg cacccctata tttttaaacc gccgatgcct
     4321 cggttaccaa agtcactggc aaggaatcga accttgcagt ttgcacctaa attttagatg
     4381 agcgatacac ctttccacca cagtgacata atgacaatag acagcaactg atgatagata
     4441 ataagaacaa tttagaagga
//
LOCUS       DACDKL010000181         4437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDKL010000181 DACDKL010000000
VERSION     DACDKL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.183"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..670
                     /locus_tag="IV329_002352"
     CDS             <1..670
                     /locus_tag="IV329_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP8941514.1"
                     /translation="FTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELL
                     DGYNSDLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSH
                     KSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRG
                     ELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSY
                     LKMN"
     gene            673..1569
                     /locus_tag="IV329_002353"
     CDS             673..1569
                     /locus_tag="IV329_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8941515.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1619..2008
                     /locus_tag="IV329_002354"
     CDS             1619..2008
                     /locus_tag="IV329_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8941516.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1995..4340
                     /locus_tag="IV329_002355"
     CDS             1995..4340
                     /locus_tag="IV329_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8941517.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
BASE COUNT         1535 a          681 c          927 g         1294 t
ORIGIN      
        1 atttacacaa gagaatggtc tggttgaaaa agaaacagaa cggttgagaa tgatttccga
       61 taaggtgagt atatactctc aaaaacaaat ttatgaaatt tcaaaaaatc aaactagtat
      121 actagaatta ctagatggat ataactctga tttgatttca gagtataaaa atgagattga
      181 aacatgtgta aacgaaataa gaaaattaaa ttgggatatt gtttctgtga aaaaagaaat
      241 acaagataag gcgaaggtag agcttgaaat tgaagattta aaaaaacaag tagaaaagct
      301 atctcataag agttataaag atgtttatga tgagtattca aaagagacag atatttatag
      361 ggaactgaaa cgtgatgtgg aggcacttaa ggaaattccc aaaaatctaa ctgatagtgt
      421 tgacaaaatt gactttggca atggtataaa agttacagac gaaattgatg aacaccgcgg
      481 agaattgatt aagaatttag tgaccaatcg ggctaagatt caaacaataa ttaatgaaat
      541 gtcagatgaa attgatagtt atagagcaga gctgaataaa tcagattgga aagttaacta
      601 taatgaggtt tcaaagaggt atcaaagagg tatcaaaaac ttagtgaaga gttatctcaa
      661 gatgaattaa aaatgttaca aaaccttgat tctttatatc aatctttatc aaaaactgaa
      721 gagcgattgg aagaaattat tcaacaagaa ataaagctta aagattacca agttgaggtt
      781 gactcagcaa tgtcaagaat gaataaatca tatcaaaatc tctattctga gagaagtagt
      841 tcctcgcgcg aaattttttc tgatattaca ggaatagagg ctactattca accaaatagg
      901 gatttcaata attatgttaa agagctcaga gcttgtatta ataaggagga cacttttgac
      961 agtgagtttg gatctcttat tgataagctg acaaaaaatt ccattactaa tcaagatata
     1021 tataatgaca tacttgatat aagagttagt ggggaaagta gtattttttc agataaaaaa
     1081 ttaatcaatt ctataagaaa attgaatgat gcacaagttc ttgaattaca aacccttaag
     1141 ccaaatgatt tagtagttat ccgacttgaa ctaaatggaa aaaaagttag tttatcgaat
     1201 gctagtgctg gtcaaaagac atctgcaatt ctttctatga ttttggcgta tggaaattca
     1261 ccactcatat tggatcagcc tgaagatgat ttggatagtc aattaattaa taacttaatc
     1321 gtaagaagta tcattacaaa aaaggaaaac agacaaattt taattgtaac tcataatgca
     1381 aatatcccag ttaatggtga ttcggaatgg ttgattagca tgggagattc aaaagaaata
     1441 tcggctgatc agtgcgggtc ggtagatgaa cccaatatca agccaagaat atgtactgtg
     1501 atggaaggtg gcgaggatgc ttttacaaat agagcgaaac gctatggttt taaacaaatt
     1561 gtagaataac actagagggt gagacatatt attatgtccg ccctttttag gaggaaaaat
     1621 gaaaaaaata aaaagctata ccagtatctg ggcagttgaa aaagtcattt acgcaatcaa
     1681 tgattttcag ttgccttttc ctgtgacttt taaccaaatg gcttggttcg ttttatcttt
     1741 actgtttgtc atcgtatttg cccatgtgcc tccactatct atgatagagg aggtcttttt
     1801 aaaatactta ggcataccgg tggctgtcac ttggtttatg tcgcaaaaga cgtttgatgg
     1861 caagaaacca ctgggatttt tacgatcctt tatcagctat cacttgcgat ttaaggtgac
     1921 ttttgcagga aagaaagtca aagaacagaa gaaacgatgg gatgaaccca gtactcttgt
     1981 aaggagtgtg aactatgtat ccgattaagt atattgaaaa taatctcgtc tttaaccaag
     2041 aaggcgagtg ttttgcctac tatgaattag tgccttacaa ttactccttt ttgtcacccg
     2101 aacagaaatt tcaagtgcat gataactttc gccaactaat tgcgcaaaat cgtgaaggca
     2161 agatccatgc gttacaaatt gccacagaaa gtagtattcg cgccacacaa gaacgatcaa
     2221 aaaaagaaat taccggtaga ctcaaagaag tagcgaaaca acgaattgat atacagacag
     2281 atgcattagt atccatgatt ggcgatagtc agattgatta tcgctttttt attggcttta
     2341 aactaattgc gacagatgaa gaagtcaatc taaagagtct caagaaatct ttcttttctg
     2401 ggcttcaaga atttgtctat ggagtgaatc atcatttgat gggcgatttt gtttccttat
     2461 ccaatgaaga aattcgctgc tattctaagc ttgaaaaatt aatggaaagc aaattggctc
     2521 gaagatttaa agtcaggcga gtcacaccga gtgacctgac gtatttgatt gaacatattt
     2581 atggcgaaaa agggataccg tttgaagaat acgaatttca gctaccaaag aaaaagctaa
     2641 aatcagaaac actggtgaaa cgttatgact tattacgtcc aaatcgttgt ttaattgaag
     2701 aaaaaccaag gtgcttacgg atggaacatg agaatcatga gtcgtacgta gcgtatttaa
     2761 caattaatac aattgtgggg gagatggaat ttccttcctc agaacttttc tattaccagc
     2821 aacaacaatt tacattccca atcgacacaa gtatgaatgt agagattgtg acgaataaaa
     2881 aggctttagc taccgttcgt aataagaaaa aagaactgaa agatttagac aatcatgctt
     2941 accaatctga caatgaaacg aattccaatg tcttagatgc tttggattcc gtggatgaat
     3001 tggaaaccac cttggatcag tcaaaagaat ctatgtacaa actgagttat gtggttcgag
     3061 tgagtgccga atcagtggat gaactaaagc gacgctgtga tgaagtgctc gatttttatg
     3121 atgataccaa tgtaaaacta gtccggccct ttggcgatat gatggggcta catgaagaat
     3181 tcttaccctc aagtaaacga tatatgaacg actatattca gtatgttacg tctgattttt
     3241 tggcggggct tggttttggg gctactcaga tgctaggaga attagaaggg atttactttg
     3301 gctacaatgt ggatacaggt cgtaatgttt acctgaaacc ggcattggct tcacaaggag
     3361 tcaagggttc agtaaccaat gcgctggccg ccgctttcct gggttctctt ggtggaggta
     3421 aatcctttag taacaatctc ttagtctact atgctgtttt gttcggtggt caagctgtga
     3481 ttgtcgatcc aaaaggggag cgaggagggt ggaaagaaac cttgccagaa attgcggatg
     3541 aaatcagtat cctcaacttg acgagtaagt cagaaaatca aggtctgctt gatccttatg
     3601 tgattatgaa gaaaaagaaa gactcggaaa gtttagcaat tgatattctg acttttttaa
     3661 caggaatttc aagtcgagac ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg
     3721 tgactcaaag tgatcaacgg gggttattac aagttattaa cgaattgcga aaagacgggt
     3781 caccaatcgc ggaaaatatc gccgatcata tcgagtcctt taccgattat gattttgctc
     3841 atttgctttt ctcagacggc tcgattaccc aatcgattag tttagataaa cagctaaata
     3901 tcattcaagt ggcggatttg gttttaccgg atgcagaaac gacttttgaa gaatatacca
     3961 ccatggaatt gcttagtgtt tcgatgctaa tcgtcatttc cacttttgcc ttggacttta
     4021 tccatagtaa ccgagaaatc ttcaaaattg tcgatttgga tgaggcttgg agttttcttc
     4081 aagtggctca aggaaaaacg ttatcgaata agcttgttcg tgctggtcgg gccatgaatg
     4141 ctggggtcta tttcgtcact caaaatagcg acgatttatt agatgaacgt ttaaaaaata
     4201 atatcgggtt gaagtttgct tttcgcagta ccgatattca tgaaatcaag aaaaccttgg
     4261 aattctttgg tttggatcag gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa
     4321 acgggattac gtccatataa ttgtgtaaaa gtgaagcatc aaaaaagcca ttgatctcag
     4381 ttaggagtac aatgtttttt gcagaaaaca tactcgaata ggactgatat caatgac
//
LOCUS       DACDKL010000182         4402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDKL010000182 DACDKL010000000
VERSION     DACDKL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.184"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IV329_002356"
     CDS             156..1862
                     /locus_tag="IV329_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8941518.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IV329_002357"
     CDS             complement(2020..2922)
                     /locus_tag="IV329_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8941519.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IV329_002358"
     CDS             complement(2919..3623)
                     /locus_tag="IV329_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8941520.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IV329_002359"
     CDS             complement(3654..4295)
                     /locus_tag="IV329_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8941521.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1349 a          947 c          761 g         1345 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac ca
//
LOCUS       DACDKL010000183         4402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDKL010000183 DACDKL010000000
VERSION     DACDKL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.185"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            34..1680
                     /locus_tag="IV329_002360"
     CDS             34..1680
                     /locus_tag="IV329_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8941522.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1671..2753
                     /gene="uxuA"
                     /locus_tag="IV329_002361"
     CDS             1671..2753
                     /gene="uxuA"
                     /locus_tag="IV329_002361"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8941523.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2861..4381)
                     /locus_tag="IV329_002362"
     CDS             complement(2861..4381)
                     /locus_tag="IV329_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8941524.1"
                     /translation="MKKKSNTKLDIFLFLSEHRFFITTQYLADHFHMSESNFLLYIKE
                     LEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESVSYQILTSLFSC
                     QTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSFSGKEVQIQYAA
                     LDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENF
                     PATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILARLSITKIESEE
                     TSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNKLYNGFLN
                     FNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDL
                     VHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISD
                     CPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         1450 a          738 c          848 g         1366 t
ORIGIN      
        1 caaaaactaa tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac
       61 tttatccctg aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta
      121 agagaaaatg gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga
      181 ggatttcatg cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc
      241 attgtttgtg aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat
      301 ttgattcttg aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg
      361 acagctgaca gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga
      421 cactttgaaa atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga
      481 ttgaaagata caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca
      541 aagaagccat cacatacaat gagtattctt gctgcgctac tttataaacg ttttttatct
      601 ggagcttggc cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta
      661 cgtacttctt tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca
      721 tttatccaat acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt
      781 atcaccccga atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat
      841 atgtcgattt tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac
      901 atccattatt taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct
      961 ggggtattct tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact
     1021 tgccttaatc ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc
     1081 atagctgagg agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat
     1141 gaaggcctcc cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa
     1201 gtaattactg tgcgcttttc taatccaaat attcctgata tgcctcagcg aatcgccacg
     1261 gatacctctc aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata
     1321 gaagttaaag acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga
     1381 taccttattg gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg
     1441 acagactttc agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt
     1501 catgagaaaa tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa
     1561 gcagagcttg gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accgaatgcc
     1621 gtacgaaaaa cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga
     1681 cttttagatg gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc
     1741 ctgcggtcaa agggattgta ggcacattga tggatatacc agtaggagag gtatggccca
     1801 gggagaggat caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa
     1861 ttgaaagcgt caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata
     1921 tcgaaaacta taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct
     1981 ataattttat gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg
     2041 gctcatccac gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg
     2101 cgcgcatcga acattccagc aatgaatttg aattgccggg atgggaacct gagcgcttag
     2161 ctcatatcaa atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa
     2221 attttgccta ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg
     2281 ctgtacatcc agatgatccg ccatactcga tttttggatt acctcgaatc gtaaagaatc
     2341 gagaagattt agattggttg tgccgcatag tagacagccc ttataacgga atcactcttt
     2401 gtacagggag tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt
     2461 gtcaaagaga tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag
     2521 atttctatga ggcaccgcat aaatccgaat acggttcatt agacatgtac cgcatactaa
     2581 aaacaatgta tgacaacgga tttaacgaat acatccgtcc tgatcatgga agaatgatct
     2641 ggaatgagac aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc
     2701 taaatggatt atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa
     2761 aactgtaatt ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga
     2821 ggttgttaat aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt
     2881 cttttttcga ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc
     2941 tcataatcac ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt
     3001 tgatatgaga aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca
     3061 taaaatcatt ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca
     3121 gatctttact gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt
     3181 ttttgtagcg atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt
     3241 ctaaaataac tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa
     3301 tggcggtttg gacttctttc aacacgctat gttcttgata aagcatgaaa gtaggaaggg
     3361 gcgtatttgg ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca
     3421 atgagtaaag aatcttttct ttttcaggta taaacaaatc aaatacagta caaaaggact
     3481 ccaccatttc tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt
     3541 gctccaagct tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca
     3601 ctaagatgcg ttgttcgcga caaattggca gattaggtcg caaaatatct acagagggat
     3661 caataaaaaa gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag
     3721 ttgcaggaaa attctcttta catattttta atagaaaata taatttatct atcataccgc
     3781 tatttagtcg gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt
     3841 gcggatcttc atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta
     3901 atgctgcata ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa
     3961 aggagataga caaaccgtac aatgctaaaa atgcattaat ctttttcatt ttcgtataca
     4021 aataagaggc actatagttt gtttgctgac ttaaactgat aatcgaattt gtctgacagc
     4081 tgaacaaaga agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac
     4141 gataatagca gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct
     4201 tatcaatatg cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt
     4261 atagtaaaaa atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa
     4321 agaaacgatg ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca
     4381 agagaaacgt tttaatcgct tc
//
LOCUS       DACDKL010000184         4396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDKL010000184 DACDKL010000000
VERSION     DACDKL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.186"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..93
                     /locus_tag="IV329_002363"
     CDS             <1..93
                     /locus_tag="IV329_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8941525.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="IV329_002364"
     CDS             complement(151..1371)
                     /locus_tag="IV329_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8941526.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..1996)
                     /locus_tag="IV329_002365"
     CDS             complement(1391..1996)
                     /locus_tag="IV329_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8941527.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="IV329_002366"
     CDS             complement(2045..2977)
                     /locus_tag="IV329_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8941528.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="IV329_002367"
     CDS             complement(3398..4258)
                     /locus_tag="IV329_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941529.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DACDKL010000185         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDKL010000185 DACDKL010000000
VERSION     DACDKL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.187"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..267)
                     /locus_tag="IV329_002368"
     CDS             complement(<1..267)
                     /locus_tag="IV329_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8941530.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQ"
     gene            complement(716..1273)
                     /locus_tag="IV329_002369"
     CDS             complement(716..1273)
                     /locus_tag="IV329_002369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941531.1"
                     /translation="MASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSY
                     HLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRK
                     QLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLE
                     QEKTLFYRQNVSQRKLERSEDEIEW"
     gene            complement(2035..2225)
                     /locus_tag="IV329_002370"
                     /pseudo
     CDS             complement(2035..2225)
                     /locus_tag="IV329_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2345..2707)
                     /locus_tag="IV329_002371"
     CDS             complement(2345..2707)
                     /locus_tag="IV329_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941532.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2711..3019)
                     /locus_tag="IV329_002372"
     CDS             complement(2711..3019)
                     /locus_tag="IV329_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941533.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3028..3447)
                     /locus_tag="IV329_002373"
     CDS             complement(3028..3447)
                     /locus_tag="IV329_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941534.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3440..3673)
                     /locus_tag="IV329_002374"
     CDS             complement(3440..3673)
                     /locus_tag="IV329_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941535.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3670..>4310)
                     /locus_tag="IV329_002375"
     CDS             complement(3670..>4310)
                     /locus_tag="IV329_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8941536.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1220 a          958 c          619 g         1513 t
ORIGIN      
        1 ttggtctaac aactgccaac cattattact aacagatctt aaataaccat taccttcatt
       61 taggcgcata agctgttcaa aatttaaaat caacacatca tttggagtgt gataataaat
      121 attttctttc agttttccga ctatggtaat tcggtcaatt gaccactgca tttttaaggg
      181 aagggtcaaa ctttgatact ttaaccgcct gttagagggg gctgttaatt tttcccactc
      241 atttatgatc gtgccttttt tactcaaatt ttgttcctcc tcaaagaaaa agagacttct
      301 ggaaagaatt ttcaaaagtt cacgtaactc ttaaaaattc attaccaaaa aatctctagt
      361 cttgaagaaa tctaaaaata ttcgtataat gattaagaag ttacggagct agtgcgatta
      421 gtttccgtaa aggtcgagaa cgtgtccagc gttgctcgac tttatttttt tgctcttttt
      481 tcagtttatt tatttgataa ctcaataata gcacttctct ttagctatgc aaatatccaa
      541 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taggttggcc
      601 aaaaaatgga cttgccattt tttagcaaat gtcagcttca gggattagtg gtttagccgt
      661 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtaactacc
      721 actcgatctc atcttcagaa cgctctaatt tacgctgtga gacgttttga cgataaaata
      781 aggttttctc ctgctctaac acttctaatt cctcagaaag ccccatatcc ttttctgcaa
      841 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
      901 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
      961 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1021 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1081 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1141 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aacaatatct ttttggaaaa
     1201 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcactc
     1261 tctcagaagc caccagagcc ttttctcgct cattttcttc tctttgccta gaaaaatata
     1321 cctcaactca tcagaaacgc tctcacgctc ttttacggcc gtttgacgca attctgaaat
     1381 ctcgctcatg atttctttat attcgtgaac atctttgatt ttgttagtac cgactaactc
     1441 acgctcccaa ccaaaatctg ccatcaaacg ttcaattgac tcaacttcct gattcctaaa
     1501 attattccaa atcactcgac cattctcttt atctgctcgt aaaccctgct gttgcaaggc
     1561 tttgttaaaa cttggttgct tttgaacacc ccgtttatac ccttcagcaa caggaacaat
     1621 attcaaatgc aggtgcggac ttacttcgtc attatgaata acagcattgt agacggataa
     1681 attaggattc cgttctttaa attctttgaa atattcaact aaaattttgt tggcaatcac
     1741 tcgattttgt tctgcttgtt gttccgatat gccagtagaa gaaccatcat cacgagtagt
     1801 acgaaaatca tcaagagtac caatttgcac gataaattct cgctgtggtt ccaatttttt
     1861 atccttgcga acctttgcta aatagtcctc gatttttcta tcggctcttt tctgctttgc
     1921 attatatttt tctacggctt caccgaataa ttcttcatat ttttcacgaa tatttgtttg
     1981 ttctaaatag atattttcat ccgattttgt gaaatcaata tgcttatgcc agtcattgat
     2041 tttttggcta tcagttaact cacgattatt atgagaaatg cttgtattct ttgttccctt
     2101 tttgaacgaa atactgaacc ccaagttaaa accctccttc ggctgtcgct aataaaaaga
     2161 gtgtaaacct ttctgttact ctttgtcaaa gagtaacatt ttcctaattg tgacatggct
     2221 gtcacaattc caaatgctct ccgagggtat tcgctgtcac aagtgacaac gaaaaaagga
     2281 gccgaaagcg tgtcacgctc tcaactcctt ttacgcttta ttttgcttca cgcaaaataa
     2341 tcgcttatcg taaattcacg tcattaaata ttgacgtaaa tttactctca ctcatctgac
     2401 ttttcttatt cggtatcact ccgaataagc cgtcagattc atcgggataa tctcgttcgc
     2461 ttggcgctga tttttcatca gcaagccact cattatccac tgatttttcg tgttgctgat
     2521 cgtcagaaat cgacgatagc caccgctcaa aatcagcaaa agtttcaaac ccaaaacgct
     2581 tccgaagagc cttgcccatt gcagcatttt ctttctcaat caagcgtttt ttctgttggc
     2641 gaagtttcgc ttgttctttt tttaattttt cttcttgcgc tttaatatct gctaaagtca
     2701 ttcccataaa ctacatctcc ttttctttgg aattaataac ggaaatttgg tttaactgca
     2761 taacatgact cattaatttc aaccaaaatt ctttttctga ttcatcagtt gtttgcgcta
     2821 aatgcttatg aacaaattca ttagtcaaaa ctgaagattt tgctaaatca gcattgataa
     2881 ccgagttgag aacatccata attcgttcaa gcatttcact ataattatct tcatccggca
     2941 aattcaaata atcagctaac cgttcataca tcaattcaat tctatcgact gtttcttctc
     3001 tcaaatcaat tttatccatg tcacacctca cttccagaat tgccaccatt ttcgattttt
     3061 taattcttga ttttctagta acaactgatc gtttttttgt tgctgaagca gggtcaattt
     3121 ttgttgatca cgaatcattt ctgttaattc agaaatatgt ttgtccttct cagctaattg
     3181 atcacgcaaa aactcattct ctttttctaa aggaaaatca ctgcctgtgt tattgaccgg
     3241 taattgacca gtcaattgat cactttttga ccgataaatt gaccgaacaa caccaatcaa
     3301 ctctttatct agcatgataa tgccattttc tttatactga gcaaggtcaa accctgaatt
     3361 gaccagttga ttgcgcactg tatttttcga tacacccaat tcgtcagcaa gattttttat
     3421 ggtcatcttt tctgacaaat cagtcattag cttctccact actttcagct accgcataaa
     3481 tatatttaac ggcgtgctta gggataataa tcggagagtc aattttatta tttttcaaaa
     3541 tcaattcata ttgcgttaca tcaagtaact taccggacat ttttttgcta tccaaaaaaa
     3601 caaccacaac gtaatcacct gttaaattta ccaaccaatt tttcttacca aaatactttt
     3661 gtttttctgt catttttctt cctcctctgt tacgtctagc cctagaagcc atgtgtctaa
     3721 ttcttttttt aaatcgtctg ccgattccgc aaaggtttct cgcaaatgat tcgtcaattc
     3781 atcattttga ctttcttgct tcaacaaatc cctgtatttt ttctttgtat tcttagctag
     3841 atttccccac tcagaatgat cataaagcaa acctttcacc ataatccgtt cagtaacttt
     3901 agtatttggc ggaaaataat gtggactagc aaagctatcc aaactttcat gaaccatctc
     3961 atgccaatca gttgcttttc cccttcgtaa ctgcaattct agtcgccacc atgtcgttat
     4021 ttccggcgga acaattttac gttttctttc ttgttccagt tttttattat acatccgaat
     4081 ttgtcgttca ctggcacgac taccccaata tgccgtttct aacttaccag aacgcccata
     4141 aattggttta aaagacacag gatcaactac ccgatattgt gtaataaact catcaggtac
     4201 atcgataatg tcgcaagcaa tatccgcacg tgaaaagtga ggttcgataa acaaatcatg
     4261 aataaaattt ttcatggaac tagctaagaa ttgactaatc ttattcggat
//
LOCUS       DACDKL010000186         4308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDKL010000186 DACDKL010000000
VERSION     DACDKL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.188"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IV329_002376"
     CDS             complement(221..1423)
                     /locus_tag="IV329_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8941537.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IV329_002377"
     CDS             complement(1426..1620)
                     /locus_tag="IV329_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8941538.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IV329_002378"
     CDS             complement(1667..2515)
                     /locus_tag="IV329_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8941539.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IV329_002379"
     CDS             complement(2720..3070)
                     /locus_tag="IV329_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941540.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IV329_002380"
     CDS             3665..4111
                     /locus_tag="IV329_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8941541.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT         1255 a          740 c          807 g         1506 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtt
//
LOCUS       DACDKL010000187         4307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDKL010000187 DACDKL010000000
VERSION     DACDKL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.189"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            84..360
                     /locus_tag="IV329_002381"
                     /pseudo
     CDS             84..360
                     /locus_tag="IV329_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            372..641
                     /locus_tag="IV329_002382"
     CDS             372..641
                     /locus_tag="IV329_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941542.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            803..1105
                     /locus_tag="IV329_002383"
     CDS             803..1105
                     /locus_tag="IV329_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941543.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1102..1263
                     /locus_tag="IV329_002384"
     CDS             1102..1263
                     /locus_tag="IV329_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8941544.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1260..1565
                     /locus_tag="IV329_002385"
     CDS             1260..1565
                     /locus_tag="IV329_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941545.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1565..1921
                     /locus_tag="IV329_002386"
     CDS             1565..1921
                     /locus_tag="IV329_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8941546.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1881..2126
                     /locus_tag="IV329_002387"
     CDS             1881..2126
                     /locus_tag="IV329_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941547.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2123..2542
                     /locus_tag="IV329_002388"
     CDS             2123..2542
                     /locus_tag="IV329_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8941548.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            2539..3096
                     /locus_tag="IV329_002389"
     CDS             2539..3096
                     /locus_tag="IV329_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8941549.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3093..3389
                     /locus_tag="IV329_002390"
     CDS             3093..3389
                     /locus_tag="IV329_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941550.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3466..3879
                     /locus_tag="IV329_002391"
     CDS             3466..3879
                     /locus_tag="IV329_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8941551.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         1590 a          653 c         1006 g         1058 t
ORIGIN      
        1 taccgccttc gaaaaaatcg aaggctaagc ccgtccgtca taaatacgga gagtataaaa
       61 atgttctttt gtcagatgag cagatggaga aactcaaaac agaattccct aatgattacc
      121 aagagcgaat cgaacgactg tcagagtatt gtgaatcatc tggtaagact tataaaaact
      181 atttggcaac tattcgaagt tgggcaagga aagaaaaaag tgaacctaag aacgcaagca
      241 gtggatacaa gcgcacagga agacgagaga agcttcctga atgggcaatc gaccaagaaa
      301 gcctatctca agaaaaaagc gctagaacga gctaatagac aatcaaaagc accattctaa
      361 gaggtggaaa aatgaagatc gattatctag aactaattaa tgaaatagcg aattataaaa
      421 agggcgagga attagacgtc ctgagagacg tatatgatca actcgaagaa gctggaattg
      481 aaggaattaa gaatgatcgt tcgagttgga gtaagctcag atactatttc gcactttata
      541 tcgatacaac acaattaaga aatttagcat ataccaaatt actatttgtt gattgtgtta
      601 aaggcttgca aaaacatctt aatgaacttg agcaggtgta atcagatgga cctaaagaca
      661 tttacagcac agattgaact aatgcatcaa gaagctttaa gacaaagtgt atcgtacgaa
      721 gacaagtggc tcaacacgtt ccatggcgga cgtgagagcg cacttgatca agtactcaaa
      781 ttactgaaag gagaatgtcg ggatggataa gaaagcggca atgcagcgaa ttatcgaatt
      841 gacttattca gaagattggc aaaatgacaa agaagctgct tcagaagtga tgaggcttgg
      901 aagagcgatg tgggcagaca agagcaacaa gccaagacca cgaaaaatcg caatttggca
      961 cggtgacaaa cttctagtga tagggacagc tgaacagtta gcaagtctca caggcttgca
     1021 cgagaaaatc gtgaggaaaa gagcaaggtg tggctacaca gacgctaaga agagaacgtt
     1081 tagatacgtg gaggaatcat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc
     1141 cttcagcgtg agattgacga tctggaacga acaaaggcag tgatggtcaa tgaaacggcg
     1201 agaaaggcaa tcgatttgca catagagaat ttaagaaggg aaatccatcg attggaggaa
     1261 tgagcgtgga taagaaagca gcaatgaaaa gaattgctga attaaccaag tcagaatctt
     1321 ggcaagaaga caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
     1381 aaaagcccaa acggagaacg ccgagaaaaa ttgcaatctg gcatgatgac cgaattttag
     1441 taacaggtac tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg
     1501 atagagctag gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga
     1561 gataatggat ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat
     1621 tcagaaagat aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac
     1681 aggagcagga gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta
     1741 caacgaaatt caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag
     1801 caagaaactt catcaagatc gtttcaaatt tgtagagaag tataaagaaa tattagaaga
     1861 gtatttgagg aggacagcaa atgataaaaa aactcgttca attcagcatg gatttatatg
     1921 atatcgaatc aggagcaaca ctatctgtgg aatcggacca tctaatcata aattttggtg
     1981 gaaagcgcca gattattttg tgggtagttg atgatgtact gtttccagaa attgttcatg
     2041 atttcgaaga atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg
     2101 aaaaatacga ggaggacagc gaatgatacc gaagtttaga gcgtgggata aacgaaagaa
     2161 cgtaatgaga gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa actttattga
     2221 atattggata aatcctaccg aattgaaatc atatcacgtg cgaaacatcg acctcatgca
     2281 atccacagga atgaaagata agaatggtgt ggatatattt gaaggggata tagtattagt
     2341 cagcgtgcga aatggcttcg attacttaga taataaagtc tgtattgtca aaaattcaat
     2401 agattattcc ggattagttt gtgccactgt tgatgaagac ttagagtatc aaatttttaa
     2461 cacagagctg tttgaagaat acacgtacga agtcaccgga aatatatacg agaatagcga
     2521 gttattggag gaacagcgat gaataaacag gaagcaatca aaaagttaga aagtattaaa
     2581 gcgataggaa atgatgcaat agctgcttgc tataacgaga gtataaattc aggtattacg
     2641 ttaatgaaaa aaatagacga accgcagaaa ccagttattc cacaactcgt ggccggttgg
     2701 cttgagaaat ctacggaccc ttttacaaaa gctgaaaaaa tagcgtattt aatcaaatct
     2761 aaagatggtg attcatatta tttctgtgat tggtttgtac gagatggcat agtgacgcaa
     2821 gagcaaggag aagaattact cgcttgggca acaagacaat catatgaaac actattgagc
     2881 ctatacaacg gctacgaggt tgagaaagag ccgttatggg caataaagaa tgccgatgga
     2941 aactatctta ctaaatgtgc tttatgggga aaagatggag taaattatag ttttgaatgc
     3001 aatccatctc atcgattgct tttcactgat aaagcaacag cggatgctgc agcattgttg
     3061 gtgaatggaa cagtggaaga ggtggtagaa agatgaaact aaaagacgga ttttacgcta
     3121 gcagtcacgg tatcggcggt ttaatgctag atatgccgac aaagaaccct aaaacacgca
     3181 agaaatcaaa attcaaagtc ggtgacatgg tccgctgtga agcagaagag ttcgtttatc
     3241 cgttcagagg atacgttaaa aagatactgt caaactcagc aatcattcgt attgaaaata
     3301 caatgaagtg tgatcgttcg actgcaaaac acaagcatta tttagctgta gcgagattag
     3361 ttgacatgga agtaatcaag agcaaataaa aaaagccgga tcgctccgac tgattcaata
     3421 aatccaacac atttattata tcacataaag gagcggtttg acttgatgca attgttacga
     3481 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     3541 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg
     3601 ccgaaggcac caaagcacgg caataaggca gaagacgcga tcattcagat gatggatata
     3661 gaagcggaga gagatgcgat tctagcggct ttgatggctc ttagtctaat tagtcgccag
     3721 atactctact acagcttctg tgtgccagat agcttctcaa actacaggat tagccgtgaa
     3781 gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca
     3841 gaagcatata aacacggaag aataattgct tataaataat ttggcggttt tttggcggaa
     3901 tgatggcggt ttttagctat ttaccagtga tattatggta gtgtcgaaag attagtgata
     3961 ggtctaagac aaaataaaat gtaagggagg aaatctccct catcgtttaa ttaagcttcg
     4021 atagacagca gcaaataaac taaaggatgt gggggtcagc tcctacagtt agttcatgtg
     4081 ttgctgtcta ttaattgcaa ctctttcggt tttattgagt atttactcaa cttaaaaaaa
     4141 ctgttgtttg tagatgtaag ctgttcctat ttgtacatag taaaaaatga tataataaaa
     4201 atattacgaa aggggtgaga gtgcatggaa gacaaaatgt atttaagaca aaaagttttc
     4261 acaaaaaaag atattgaaaa tcaaattaat ggatttatac agaaatt
//
LOCUS       DACDKL010000188         4291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDKL010000188 DACDKL010000000
VERSION     DACDKL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.190"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..894)
                     /locus_tag="IV329_002392"
     CDS             complement(<1..894)
                     /locus_tag="IV329_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8941552.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALS"
     gene            1531..1617
                     /locus_tag="IV329_002393"
     CDS             1531..1617
                     /locus_tag="IV329_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8941553.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(1738..2427)
                     /locus_tag="IV329_002394"
     CDS             complement(1738..2427)
                     /locus_tag="IV329_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941554.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2434..2712)
                     /locus_tag="IV329_002395"
     CDS             complement(2434..2712)
                     /locus_tag="IV329_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941555.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(2672..3586)
                     /locus_tag="IV329_002396"
     CDS             complement(2672..3586)
                     /locus_tag="IV329_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8941556.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(3568..3948)
                     /locus_tag="IV329_002397"
     CDS             complement(3568..3948)
                     /locus_tag="IV329_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8941557.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1112 a          827 c          781 g         1571 t
ORIGIN      
        1 cgaaagtgcg gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat
       61 ttgttggagg agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc
      121 gggttcttcg tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt
      181 ggttggtgca tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg
      241 atagccaata acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt
      301 gaagattgca gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg
      361 cttataactt ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa
      421 acctttggtt ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag
      481 ctcgttgaat tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag
      541 tgctttttcg taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa
      601 ttctgtaaac attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg
      661 taaagataag attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt
      721 tgctttgtag ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc
      781 tttttcacgc attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt
      841 tcttaaagga attgtattta attcatggtg gtattttaca atctcattag ccatataatc
      901 acctcaatcc aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca
      961 aacagacaaa atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc
     1021 ttcataaaca gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac
     1081 agcttacggt aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta
     1141 attttcccgc ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac
     1201 tgccaatcca aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac
     1261 attgtactgc gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt
     1321 tgagcggttc tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat
     1381 ctagctttaa agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga
     1441 tacttaaaaa caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga
     1501 cagtcttgcg actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt
     1561 atagaagttt gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat
     1621 caataaaggc gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt
     1681 cgtgtatcta atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca
     1741 attttgctgt tgctgatcta accattcctt gacacttttt agataattta atgccgtttc
     1801 ttttttcgtt ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta
     1861 ggtaactgat tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc
     1921 actgaataaa ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt
     1981 tttggttcgc tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata
     2041 aagacgagtg agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat
     2101 atcgttcatt tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc
     2161 ctttttcttt ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact
     2221 tcttgaaata cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa
     2281 tgtaaatata acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga
     2341 atatcttgtt tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat
     2401 gagtagatag ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc
     2461 taacagtgtc atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat
     2521 tacttttttc tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg
     2581 tgaatcaatt gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt
     2641 ctttatttca ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg
     2701 ctcctctttc aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag
     2761 tcgtaagagt tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag
     2821 ctcttcgtag ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat
     2881 gtaaaatcgt ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt
     2941 tgtggatcaa ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt
     3001 taagagaatc acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa
     3061 tcgcaaaccg aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag
     3121 acatggttcg tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac
     3181 aaatctcgtc atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt
     3241 tgttcccagt ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt
     3301 ctgtatgtgt gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc
     3361 ctagttgatt ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt
     3421 gtgcttgtac acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg
     3481 cataatcaat gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga
     3541 tcgctcggct acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc
     3601 cttttgcatg atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg
     3661 ttggtgtgtg ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc
     3721 gcgcgcgatt gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg
     3781 tgccttggct ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag
     3841 ctttgcaaat tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt
     3901 atatttttta gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct
     3961 ttcagtacgt cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa
     4021 gtaaagctca ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac
     4081 ctacgtattt ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc
     4141 ctccccccca ctctccacaa tacaaaaaag actagcaaat gctagccact ttttgtaaag
     4201 tttcgcttgt ttcaccttcg gtgaaattgt tgcaaatcaa taattaggta gagctacatt
     4261 tttttcgttg ttcaaagatt cttatgccat t
//
LOCUS       DACDKL010000189         4256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDKL010000189 DACDKL010000000
VERSION     DACDKL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.191"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..1372
                     /locus_tag="IV329_002398"
     CDS             71..1372
                     /locus_tag="IV329_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8941558.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1399..1659
                     /locus_tag="IV329_002399"
     CDS             1399..1659
                     /locus_tag="IV329_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8941559.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2658..3893)
                     /locus_tag="IV329_002400"
     CDS             complement(2658..3893)
                     /locus_tag="IV329_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8941560.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1231 a          682 c          881 g         1462 t
ORIGIN      
        1 ttagttatga acattctttg cgttgtcatg caatgaatgg ttcatatttt attgaaaagg
       61 agtggtttta gtggaaaagc tgatgaaatg ggtcgaagag aaacttgtcc caccaatggc
      121 aaaaattggt acacagcgtc atttattagc aattcgtaat ggggttgttt caacattgtc
      181 attgattttg attgggacat tttttatggt ctttatcaat ttaccgtttc ctggatggaa
      241 tgaatttatt gccccttatt cggcaacgat tgttttaccc tttaggatga cgatgggatt
      301 gatggctatt tatgcaactt ttgttatggg ttcggatcta gctaaatcat atggattaga
      361 tagtgtgaca ggtggtatat tatctttagg aacgttcttt atgttacaag ttcctgtaaa
      421 cgttttaact cctgaagagg cacctcttgg ctgggtctta cctatgagtt ctttgggagc
      481 atctgggatg tttgcgggga tcttatctat gatttttgca gtggaagtat atcgtttctt
      541 taaaaatcga aatattacta tcaaaatgcc agaacaagtg ccacctgctg ttgcccgtag
      601 ttttgaggca ttgattccag gtgcagtcat tttaacaatt acttggctga ttcgaagcgt
      661 cgcgggcttt gatgtaaacg ctgccttatt atcgttattc gagcctttaa ccaatatttt
      721 agggaataat ttgcttggtg ttttactacc aatgtttttg atccatttgt tgtggtcatt
      781 tggtatccat ggcatgagta ttgtaggggc tgttgttcgt ccaatgtggc tgatcatgtt
      841 ggatgagaat gcaaaggcat tggcagatgg tacagctgcc acccaattac cctatatcgc
      901 tcctgaacag ttttatcaat ggacggtaac aattggtggt gcaggagcaa cgatcgtggt
      961 atctgttttg tttatattct tctgtaaatc aaaattcttg aaagaggttg gtcgtttttc
     1021 tattattcct ggtatcttca atatcaatga gcccataatt tttggagctc ctatgatgtt
     1081 gaatccttac atgttcatcc cgttcaactt agttccttta gttttgacta ttatcagtta
     1141 ttttgcagtc aaattagaaa tggtcaatag atttactgtt ttgccaacat ggacattacc
     1201 agctccaatc ggcggttatc tttcagcagg aaatgactgg cgtgttgtta tcttgatcat
     1261 gatcaacaca ttgatagcat ttattatcta ttatccattt gtaaaagctt atgataaaaa
     1321 gatgctgtca gatgaacaaa gtcgtggaga aaacgaagtc cagactgtct aagtttggtt
     1381 tttagaaggt gggaaacaat gtattggctg attttatttt ttgtttttat ctttttatta
     1441 acggctagtc accttatatt gaatatgcta gctacatatc atattcaaat caatcgatgg
     1501 atttgggcac tggcatcatt tttgatcgtg attcttccaa agatcattgt accgcatatg
     1561 aatgttttgt tttcatgggg gacatatgta ctttgtggaa tttttgctat caattttatg
     1621 atcgagcagc atagatggtt tgtcacatcg aagctttaaa tgttctaaaa gtggttgtaa
     1681 cagaagtggt tagcttcaag aaataagaag aaatttacga aaattgcttc ttaaattttt
     1741 gtgaaatgtt ggcttatttt tcgtttggtt gaacttatta ccgcagatgc aagcttttga
     1801 ccaccgttta ttcggaatat gggactgtga caaggctttt gtcacagccc ttttttacat
     1861 aaagattatt attgttttaa ctgttttggc agaaagaagg aggaaaaaat ggaaatttta
     1921 tcagcaatgg atttagaatt agtagatcta ctaccaaagt gatcaagtca aatatagatt
     1981 tgggagctta caaccatata gtaggggctg cagtatggtg tatgaatggt aatatgtatg
     2041 atgtgagtgt ttattcaaat catgaaacgt gtatagaaat aattgtgctg ggaatagcaa
     2101 tcagtcatgg tgaacgtgac ttttagtgta tcgttgctga cgacatagaa cattctgaca
     2161 tgatctactc aatatgtggg gattgctggc agtttatata ggagtatgca tcggattgcg
     2221 agatcattgt ctcaacagaa gagggaggtt gattatcaaa gataaagtgg taaatcctac
     2281 atttgattta gtaattgttg aggggactag cggcacaaat ctctacaaag cgatgcttct
     2341 agatctatat gacacatttt aagaaaatgt atttgcctaa ttattatggt ctttcttttg
     2401 aagaaacgtt gagatgatcc aaccagagaa aggtgtcaaa gaatttgact aagaataaat
     2461 ggcagacgct acctatgctc atttggatag cgtcagatct tttattttaa ggatgatttt
     2521 gtgtttttta gacaccgtaa tttaagtaac tctaactttt caatccacta tttacttttt
     2581 ttgtattttc actgtctggg tctcgaatag cgaaattact gatgagtctc tattaaaaaa
     2641 gctttttcta cttgaaacta tattgggtgg ttgctcagga aggtttcgat ttcttgaatt
     2701 gcttgttgtg gaccatttat tggaaaaacg gcttgcagcg tgcgtgcctg ctgacgaatc
     2761 ttgggtgaag aaacttcgcg cagagcaatc tgcagttttc tttcagtcac tggttttgtg
     2821 agggcgattg cgtttccgta ttttacacaa tagtcaatat tacagctttg ctcaaattgc
     2881 atccccacac cgataaacgg tttgccggat tcacaggcag tttggacggt cccttccccg
     2941 ccgtggataa aagaaaaatc aatgaattca tgcaaaagat gactagacag atattctgtt
     3001 attaaaagat cttctgagtc agattgaatg tcatcaagca gatagtcaat aggtgcaata
     3061 accgctagat ccggttgttt ttgtaagaag aacaaaattt tgcgtaacat acgcggattg
     3121 cctgaactac ccattgcgaa atagataata cgttttcttt cttgttgctt tcgctgtata
     3181 aaagttagca cttcttcagg caaggaagta ctgagcttag caaaaattgg accaacgatt
     3241 cgataatttt ccggcagctg ttcttcttcc aaaaatagtt taggcgttgt gatgagattt
     3301 agatcagcat ccatcatttg taaacttgtt tttggtaaag gaagcatttc tttttgtgca
     3361 gcgtgtttga aggcattggg gatccatcgg gtatttacta tacgcttttt tgtccagtgc
     3421 caaaggggat tgcttaaagg gccgaagaaa cgtaactcgt ggggcatcag cgcatgctct
     3481 gattgaaaaa aaggttcact caaagcatag ggtttaacat atactaaagg aatcttttta
     3541 ttgcgggccg ataaaaaaat tgtcacattc gaacctgtca caatcagtcg aggctcaaac
     3601 ttagcaatca gactctgttc actagtgata cgagaggcaa ccatttcttt ggtaaaaggg
     3661 tttttaagtg tacgcaactg gtcaaatttt atgatttgtt cttcttgttt tttcgtaagg
     3721 atcggcgcta gtaaatgata ctcaaagcca ctttctgtga tcaaaggagc aaaagtttta
     3781 gaaaagccaa agaatacaca gtaataacgg tctttcatag ctttggcaac ctcaatcatt
     3841 cgtgttgtct cagctaaatt gaatactgca ggagcaaaaa gaattgtctt catgaaatcc
     3901 tccttattgg aatgagtcta tcccttcgtt tcttactagt atactaaaaa acacagcgtt
     3961 cgagtagcct atgtatattt tgaatattaa actgtctctc gctgttttca caacttctaa
     4021 atttaaattt tgtaatcaag ttgaaactcc aattttcaaa aaaacacgca acaatttttt
     4081 gatcgattta tatgttgtgc cagataaata cgagaagttg tgtcattaaa aataaacatg
     4141 agaacataaa aaaataaaat aattatatta attgataggt gtgctgtcgt cattatacac
     4201 acgtaagaaa gaaatagtta tgtttaaaaa tattcttaga ctttctcgcc atgaca
//
LOCUS       DACDKL010000190         4232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDKL010000190 DACDKL010000000
VERSION     DACDKL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.192"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..189)
                     /locus_tag="IV329_002401"
     CDS             complement(<1..189)
                     /locus_tag="IV329_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941561.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVRE"
     gene            complement(397..1323)
                     /gene="glsA"
                     /locus_tag="IV329_002402"
     CDS             complement(397..1323)
                     /gene="glsA"
                     /locus_tag="IV329_002402"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8941562.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1346..2773)
                     /locus_tag="IV329_002403"
     CDS             complement(1346..2773)
                     /locus_tag="IV329_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8941563.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2804..4018)
                     /locus_tag="IV329_002404"
     CDS             complement(2804..4018)
                     /locus_tag="IV329_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8941564.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1315 a          905 c          753 g         1259 t
ORIGIN      
        1 ctctcttact tgttctttta tacaatattc tagacactcg gctagacttt ctagatcgtg
       61 aaggatcgtt ggtgtagtca cccttagaga aatagctgtt tgttcgatgt ctatttcttg
      121 gaattcaaat aattgttcac acagatacac ttttctaaat atatttcgct cgatgtaatt
      181 ttccagcatt cctgatacac gcctccagtt tttcttatga ttttatttaa tcatatctgt
      241 aaatcgtatt tttgtttcct ttttatttag aaaagccctt tcaaaaacca ataaaaaaag
      301 aaactgggac aaaagtcaaa caagctcaca atttcagcct cattgtaggt agctttgagc
      361 cgtttggact tttaccccag cctctttcaa gaatggttat tcaaagatgt cagcgtctag
      421 ttctttgact acatctttca acaattggat accagctgcg ctgttaccaa atggatctag
      481 tgccgggctg aagacaccta tgccgtaacg atttggcaca gctgccatca acccgccgcc
      541 tacaccgctt tttgcaggta caccaacatg gacggaaaac tctcctgatt catcatacaa
      601 accagcagtt gtcataattg atttgacgat tgtagcactt tcttcagtaa tgatttgttt
      661 gccattccaa ggagcaattc ctttatttgc cagcacagca gctaattttg ctatgcctgt
      721 agcggtgaca ttgagggagc attgtctgaa ataagcatcg agtatctccg gtacgtctcc
      781 ttcgatcatt tgattacctt tcatatagta agcaagagag cgattgatat cacctgttct
      841 tgattcagaa tgatagattt cttcattcaa tgtgatatta ggatcatcac agatctcttt
      901 cataaatgac aagattcgct caaatttttc atctgcatct ttaccggcaa tcaacgaggt
      961 tgttgcaatc gctcctgcat taacaaaagg attcattgga tgtttgcgat gattgatttc
     1021 catattcatg atagaattga aggcaaaacc ggttggttcc gtgttgattg tttgaaagac
     1081 tttttctatt ccgttgtctt tgattgctaa taacaatacg ggaacttttg acatgctttc
     1141 gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata
     1201 aatcgccata cctaattgtt tgggatccac ttcagctaat gctggaatgt aatccgccac
     1261 ttgtcctgtt tgataatagt ggaaattttt tccaatcaat tgttctaatt cttttacttc
     1321 catttgaata accagctttc attttttatt tttctgcatg ctggaattta tgacggcatg
     1381 cgtagatcgt aaatggaata actaagacga taagataaga aacaatcaga atcgtttcat
     1441 aaatcccatg ctgtccagct gcgatactag caggtggaac aaatgagata caaaaagcaa
     1501 aaagtgaagt caaaagacct aaacaagcaa cgattacttt tacaactttt ccgcctggaa
     1561 cctgataggc tctttcaaga ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa
     1621 acataaagta caacatcagg tagattacaa cagtcaatga catcgctgca agaaatgaaa
     1681 gattattccc cccgccgctt aatgtaaaga cggctgccca gattgttgca acgatcccat
     1741 ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa
     1801 gtaatccttc ttctgctgct acgtgaagtg cttttgaagg agaggttacc caagaagcaa
     1861 tttctccgat gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc catgcaagat
     1921 ttggatcaat gtgtcttaat aggaattcta acgattgaat cacccctgta ttaagagaca
     1981 aaccatgttg aggtactgta gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg
     2041 ccagaataac caatacaaac attgctaatg gataatcctt tttagggttt tccatttcat
     2101 ttgcatgact ggcagatgtt tctacgccca tatatgctaa aataaaggaa acaaaaacaa
     2161 ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt catagacagt atcgtttgta
     2221 gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa caagactaat gctggcaaca
     2281 agataccagc tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt ccttttaact
     2341 gtgagagtaa aatagcccag aaaataatca ataggattgc caatttcaga tatttattgt
     2401 tttgtatagc accaataccc gttgcatagg ataatgcacc agttaagaaa tacagcatgg
     2461 tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt
     2521 ttcccaatgt gcgactgacc caagtataga caccgccttt ttcccatcct ttcaccgttg
     2581 ccatctctgc tgcgcacaat gccacaggga taaaccatag caaaccgcct aataacaaga
     2641 aaaatactaa gctgaagcct gaggtcgcga atgtgggata ttcatataat gaaacgacca
     2701 ttgagatggt catagccaaa aagccaaaga acgttaattt ttttgcagga attttcgttt
     2761 tgctttgttg catgaatctt cctcttttct atttgaattt gtttcaattg agtttgttgc
     2821 caaagaagaa aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa
     2881 agtgcatcgc cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga
     2941 tgacaccaat cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc
     3001 tgttagatgc aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac
     3061 cccacacatc aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac
     3121 agacgatacc cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac
     3181 aaggcgtaat ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc
     3241 catttccttt tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga
     3301 atgttgaacc aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa
     3361 aaccgaacca gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat
     3421 gctgacttct ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag
     3481 agccgaatcc agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct
     3541 gatccaaaaa tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc
     3601 aaaaaataac aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca
     3661 tcaaaggaac ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa
     3721 tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg
     3781 ccgggtttcc aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata
     3841 cccacataat cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat
     3901 tattcttccg tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca
     3961 ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt
     4021 ttttcgcctc ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg
     4081 tttttttgaa ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt
     4141 tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata
     4201 gaaaaccgtt tattttcgtc tttttttatt tt
//
LOCUS       DACDKL010000191         4203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDKL010000191 DACDKL010000000
VERSION     DACDKL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.193"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..354)
                     /locus_tag="IV329_002405"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="IV329_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="IV329_002406"
     CDS             complement(614..1162)
                     /locus_tag="IV329_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941565.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="IV329_002407"
     CDS             complement(1163..2020)
                     /locus_tag="IV329_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8941566.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="IV329_002408"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="IV329_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="IV329_002409"
     CDS             complement(2582..2911)
                     /locus_tag="IV329_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8941567.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="IV329_002410"
     CDS             complement(2988..3260)
                     /locus_tag="IV329_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8941568.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="IV329_002411"
     CDS             complement(3260..3523)
                     /locus_tag="IV329_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8941569.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="IV329_002412"
     CDS             complement(3629..4045)
                     /locus_tag="IV329_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941570.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="IV329_002413"
     CDS             complement(4051..>4203)
                     /locus_tag="IV329_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8941571.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DACDKL010000192         4152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDKL010000192 DACDKL010000000
VERSION     DACDKL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.194"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..904
                     /locus_tag="IV329_002414"
     CDS             <1..904
                     /locus_tag="IV329_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8941572.1"
                     /translation="KRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFN
                     EDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLS
                     INELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLN
                     LLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAM
                     PHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASN
                     HELLANLKTSSSFEKLVYILKEGL"
     gene            905..1351
                     /locus_tag="IV329_002415"
     CDS             905..1351
                     /locus_tag="IV329_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8941573.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            1367..1681
                     /locus_tag="IV329_002416"
     CDS             1367..1681
                     /locus_tag="IV329_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8941574.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            1707..2756
                     /locus_tag="IV329_002417"
     CDS             1707..2756
                     /locus_tag="IV329_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8941575.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            2768..>4152
                     /locus_tag="IV329_002418"
     CDS             2768..>4152
                     /locus_tag="IV329_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8941576.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPAL"
BASE COUNT         1461 a          592 c          864 g         1235 t
ORIGIN      
        1 taaaagactc aaattgggag taaatatcta taacccttat acggacaaaa tcaaatattc
       61 ttttaaaagg tcatttatga taagtgtaga tttattagag gaaatcgaag agagattctg
      121 tatacatttt aatgaagatg aaatcgctta cgtaacactc catattcaat cacttttgga
      181 tcgttacaaa cctgacaaaa cgaaagtgat attagtttgt agtagcggat atggaacatc
      241 aaaattatta gagcaaagaa taaataacgg ttttgcagat atggttgaga tagaagatgt
      301 actaagcatc aatgaactac aagatagaaa agtcactgat gaacttgtta taagcacgat
      361 tccgattgaa aatacaaatt ttcctgttat tgttgttagt cctttgatgt taggggcaga
      421 tgtcagaaaa gtaaaacagt atctacagtt aaatgaacaa acagaagatg cttttctaaa
      481 tttattagat aaacgatttt gcttttttac ccaccaaata aaggataatc aagtgaacgt
      541 tttaaatata attaccaaaa aattggtaaa agaaaattac gcgaaagaag ggatactaga
      601 gggagcaatc aaaagagaaa aactatcttc aacagcgatg aatgattttg ctatgccgca
      661 tatcgaagta gattttataa aaaagccagt aatctgtgtc tacgttaatc cagaaggaat
      721 tgattgggat gggacacttg ttcattctgt tttctttttt gcacttaatg aaaaagtaaa
      781 acctgaattg aataaaattt atgaaacatt caatgaaatc gcttcgaatc atgagttatt
      841 agcaaattta aaaacgagtt cgtcgtttga aaaattagtt tatatattga aagaaggctt
      901 ataaatgatc agtgaaaaaa tcaatataaa taatatagtg atagatttaa atgcatctga
      961 taaaaaaagt gtagtcgaaa aattatcgaa attgttagag actaatgggt ttgtgtctga
     1021 ccacaaacaa tttcaaaaag acgtatttga cagagaaaac catatgacca caggaattgg
     1081 gaaaaatatc gccattcctc atggcaaaag cctagcagta attgaatcaa cagtggctgt
     1141 agccaagtta aagcggtctg ttgaatgggg cgcattagat gatcaaccag ttgatctcgt
     1201 ctttttgtta gcaataaaag acactgataa aggtactaat catttacgta ttttagcaga
     1261 attatctgga agattgatgg atgacgagtt tgttaaaaga ataaaagatt cgagaaataa
     1321 aaaagaattg gtaaaagctc tatcatttta aaaggaggaa tttcatatga caaaaatagt
     1381 agcagtaaca gcatgtatcg cagggattgc ccatacttat atggcaaaag ctaatttaga
     1441 aaagtttgcg aagaagaaag ggttagatat taaagtagaa acacaggggg ctatggggat
     1501 agaaaacaaa ctttcaattt cggatgtttc cgaggcagat gttgtcatat ttgcagtaga
     1561 tacaaatgtt tcagaacgag gtcgctttga cggtaaaccg attttaacag tagggacctc
     1621 agaggtagta aaagatggtg aagagacgat tgatgaagca attgcattaa ttaacaaata
     1681 aaaaatatat cgtgggggag ttactaatgg aattcaagaa acaaggaaga gaaattctaa
     1741 atcatttcca aagtggggtt tcctatatga tcccgcttgt cgtagcagca gggttactaa
     1801 cttctattgc agtcattttt ggtggcacag gtgtttggga ccagacggat acattctggg
     1861 gagtcctgcg aatgattggt cagacgggat tacagtttat cgtaccgatg atttcagcct
     1921 atatcgcata ttcaattgct gaccgtcccg gtctagcacc agcttttatc actgggatga
     1981 tgtgtcaaaa tcttggtatg ggatttattg gaggaatggt tgctggatta gcttgtggct
     2041 atttagctaa tgcattgaaa aaagtaaaag taccgatgca agttattagt ctaaaatcaa
     2101 ttatgctgat tccttttgtt actacattag ttgttggaat catattatgg tatgtactag
     2161 ctacgcctat tcaagcaatg acaacaggac tgactaattg gctagaaggc atgtcaggag
     2221 caaatgctgc tttgatgtcg gctattttag gaggaatgat ggcgtttgat atgggaggac
     2281 ctatcaataa aatcgcatat gcttttgggg ttgcttcttt ttcaagcgga agctttggac
     2341 ctagtacagc catgttatta ggaattgcta ttccgccatt tggaatgttt ttagcaacaa
     2401 ttttaaacaa aaaactttat gatgaatcgg aacgagataa tgcaaagaca gctatgatta
     2461 tgggccttgt ggggattact gaagggacga tcccgtttgc cgtgaaagat cctttacgag
     2521 taataccaag catcgtggtg ggcactgcag tagcatgtgg tctaaatgga ttttttggtg
     2581 ttactcaaga aacaatgctt tcaaccttta tggcaattcc ttttgttacg aatatacctt
     2641 tatatttagt atcaattgtt attgggggag tggttacagc gttaatggtc aatgcactaa
     2701 aatccatcaa gtataaaaaa gaacaacaag taaattcagt agaagaagga aagtaggaga
     2761 atctgatatg gtaaatgcac atataattca acacacgcac tgggacagag aatggtattt
     2821 tacaaatgaa gatgccatcg tattgtctga tcaggtcttt actgaagttc tagatgaatt
     2881 ggaaaggaac cctaaagtaa acttttgttt agatggacaa acttcaatcg tcgatgagta
     2941 tttggagatc aatccgcaaa agcttaaggt tatccaacga ctgatgaaaa caggacaact
     3001 attcgtggga ccgtggtatg cacaaacaga tgccttatta gtggatgctg aatcaatttt
     3061 aagaaactta atgattggaa tcagtgatac gatccttaaa tatggagatc caatgatggt
     3121 agggtatttg cctgacacgt ttgggttcaa tgcgaatttg cctacacttt tgcatcaagt
     3181 agaaatcgat aatttcatgt gttggcgagg attaaacttt gaaacgatgg ttccatctcc
     3241 ttattttatc tggaaaggat tgggagataa atttgtttat gccatgaatt ttccatttgg
     3301 ttatatgact ggaatgatga ccttagacgc aattaaagat attcctgaat ttgcaaaaga
     3361 aaagttggat gaagcagttg aaatgcttca tatgcaagga aaaaacaaag atattttagt
     3421 accttcgggt gtagatcaaa agtcaatgat tttaaatttt gatcaagtcg tagagaagtt
     3481 gaataaatac agtaaattcc acaatattat ttcagattat ccttcttatg tagaagttat
     3541 cagaaacaaa aaagaattac ctgaatataa aggtgaactt cgtcagccgg tttactctcg
     3601 tgttcatcgt tctattggct cagtaagaac aaaaatgaaa atagaaaatt ttaaattgga
     3661 acaaaaaatt ctaaaacgag ttgagccgct catgctgatt gcagaaagaa atggtatcca
     3721 tatcagtaaa ggcctacttc gtcggttatg gaaaaagatt ttgcaaaacc aggctcatga
     3781 tagtattggt ggctgtgttt cagataatgt tgcggaagat attttccatc gaatcaaaga
     3841 agctaatgag atagctgacg gaatcgaaaa ccttattgta aaacggatgg ctgatgcatt
     3901 aaagctaaaa tcgaatgaag tccttgtttt caacacaacg actgagaatt atagtggatc
     3961 gaaaatcgtg catgtagtag ctgattcaaa gaaaatacac ttcaaaggta gtccctataa
     4021 tatcattgaa aaagaagttt attttccaga acggaaaaat gcttatcgga tgattgctac
     4081 aggtttcgaa tattttgatg aaccagctta ttatgaactt gacataaaaa taaatgtaaa
     4141 tgtgccagca ct
//
LOCUS       DACDKL010000193         4076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDKL010000193 DACDKL010000000
VERSION     DACDKL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.195"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            250..621
                     /locus_tag="IV329_002419"
     CDS             250..621
                     /locus_tag="IV329_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8941577.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(771..843)
                     /locus_tag="IV329_002420"
     tRNA            complement(771..843)
                     /locus_tag="IV329_002420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(808..810),aa:Gln,seq:ctg)
     gene            complement(889..1317)
                     /locus_tag="IV329_002421"
     CDS             complement(889..1317)
                     /locus_tag="IV329_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941578.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(1377..2135)
                     /locus_tag="IV329_002422"
     CDS             complement(1377..2135)
                     /locus_tag="IV329_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8941579.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2430..2627
                     /locus_tag="IV329_002423"
     CDS             2430..2627
                     /locus_tag="IV329_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941580.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2733..3215
                     /locus_tag="IV329_002424"
     CDS             2733..3215
                     /locus_tag="IV329_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941581.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3273..3869)
                     /locus_tag="IV329_002425"
     CDS             complement(3273..3869)
                     /locus_tag="IV329_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8941582.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1278 a          655 c          785 g         1358 t
ORIGIN      
        1 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
       61 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
      121 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
      181 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
      241 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
      301 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
      361 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
      421 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
      481 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
      541 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
      601 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
      661 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
      721 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
      781 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
      841 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
      901 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
      961 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     1021 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     1081 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     1141 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     1201 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     1261 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     1321 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     1381 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     1441 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     1501 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     1561 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     1621 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     1681 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     1741 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     1801 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     1861 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     1921 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     1981 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     2041 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     2101 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     2161 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     2221 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     2281 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     2341 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     2401 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     2461 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     2521 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     2581 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     2641 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     2701 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     2761 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     2821 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     2881 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     2941 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     3001 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     3061 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     3121 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     3181 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     3241 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     3301 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     3361 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     3421 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     3481 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     3541 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     3601 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     3661 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     3721 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     3781 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     3841 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     3901 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     3961 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     4021 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttga
//
LOCUS       DACDKL010000194         4006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDKL010000194 DACDKL010000000
VERSION     DACDKL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.196"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..128)
                     /locus_tag="IV329_002426"
     CDS             complement(<1..128)
                     /locus_tag="IV329_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941583.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFH"
     gene            complement(131..487)
                     /locus_tag="IV329_002427"
     CDS             complement(131..487)
                     /locus_tag="IV329_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941584.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(785..1759)
                     /gene="sgrA"
                     /locus_tag="IV329_002428"
     CDS             complement(785..1759)
                     /gene="sgrA"
                     /locus_tag="IV329_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8941585.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1955..2794)
                     /locus_tag="IV329_002429"
     CDS             complement(1955..2794)
                     /locus_tag="IV329_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8941586.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2979..3866
                     /locus_tag="IV329_002430"
     CDS             2979..3866
                     /locus_tag="IV329_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8941587.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1340 a          802 c          591 g         1273 t
ORIGIN      
        1 aagtgaaaaa agtatcggat acggtattct tctccttcta tcggattacc ttttttcaag
       61 ctgcaagata aatgatagtt tttcaaaata gtattaagtt ctcttaattt tctttgaatc
      121 gtacgcaaac ttatataatt ttttgactaa gattttctaa ggattgataa gtacctttgt
      181 aaagtgaatc cagtaaaata taaccaatcg ttccctctaa aagtatagaa taaactttac
      241 tcagtaaaaa ctgagaagaa aactcgactt ttatcatctt gttttctgaa agaaaagata
      301 atttatattc tggatacctt tcgcaaaacg attctgcttg ttgaatcacc gaattcatct
      361 tagccagggt gtagtttaga tctaaagcga tatccttcac cacatataat ttctcgtctc
      421 tttcataaca atagttcaaa aattgatata cttgcctttc attgatatca ataaattctt
      481 caaacaaagt acatactcct ttttatctat tatttagact tattccgaca gctacaagaa
      541 taacgtacaa atagattcgt tactgatttt tcagagatac taccatgcgc agtgtgcatt
      601 gtcaattatt cttgctctta ttttccattt gtgttcctgt tatttcctgt ttctcaaata
      661 tgttagtaaa caaaaataga tctggcaaag ctacaacttc gccaggcaat acagcactct
      721 cgaatgaaat attcactcaa aaatctatcg ttttattcaa ttttagatct gttgctttta
      781 ttctttattt cactcgatgg aagagagaac gaaaatagct tgctcctaca atcaaaatga
      841 gtaacacacc catccatgta attagcttgt tcaaggttct actaccagtt tctggtaaat
      901 cgctttttct attatctgtt tctttcgctt tttcttgatg atcaattcca cgagaaacaa
      961 aatcaggctg ttttagcaca ctttttaggt tgctccctgc atttctcttt tgttccttag
     1021 ttggtatgat aggtaccaaa tcaagttcat tatttacttt tcctgtgctg ctttcgctgc
     1081 tcgtgcttcc tgtgctactc tcactactca tgcttcctgt gctactttca ctactcgtac
     1141 tctccgtact gctttcgctg ctcgtgcttc ctgtgctact ttcactactc gtactctccg
     1201 tactgctttc gctgctcgtg ttttctgtgc tactttcact gctcgtactc tctgtactgc
     1261 tttcaccgct cgtgttttct gtactgcttt cactacttgt gctatccgta ctgcttggcg
     1321 gtccaacaac tgtccaactg cttggtttca aattctccgt gatcgtcatc tgtccatcat
     1381 aaaaaacaat caaatcaact ttatctttac tcttgatctc atcaatcgat agttctgcag
     1441 aaatcattat tgtatactca ataccagcgg tccattgtac gtcttttaaa taaacttccc
     1501 caataacttt ttctgaactt ccattaccat cccaaccata tgttagaaca gcatgtacat
     1561 cactagcatt cacgcttttg tttggtttga ctggaatagt aatgacttgt tgtgtcatgt
     1621 cataaacaat ccgatcagtt ttcacagtaa atgaaagatt ctcatttgcc cgttcatttg
     1681 cagaagtact gattccaatc attgctgcaa ttatactcag cccaagaata acaaataata
     1741 cggtcgctgt tttcttcatt tctatcactc tccatataat taccttctat ttgataaagg
     1801 aattatataa aatgaaaatg atgagaacac gacagtcagt tgtgaaaatc taacaaaaga
     1861 tgtcgtgtat tcgcttgaat ttgtttactt tttcgtttaa tcaatgacag acttaaaaaa
     1921 gcaagaccga tatcagcctt gctttcttca tcttttattg ataaacagca gtgtatttct
     1981 tcatccgctt tgttttatat gcatagagta acaaaccaac aatcaagact aacaagaata
     2041 atgtagttga tatagatgct tcagccccaa agtttccccc agtaattatt gtattttgcg
     2101 attggatcag agtggagaaa aaaccttgtt cttgcgttcc gctgactggt atacccaaaa
     2161 tagctccttg tgtccaattc caagtaatat gagcaccaat agccgtaaat aaacttcctg
     2221 tactgtagat aagtgcgctg aaaaagcaac ctgctaaaaa gatattgaaa actgccagaa
     2281 atgtcatgtt aggattcaat ccgtgcaaaa gagcaaataa tgcagcagat ccaatcattg
     2341 ctattaacgg accaaatcta agcatctgat gctgaaaaaa gccacggaat aaaagctctt
     2401 ctgatgcccc cactgcaata aataaaataa aactcgttat cagcggcaca atgtttgcgt
     2461 ttcctgaatc taaccgatac gcccccgtca aatataatgg actagtaata agcagaaaca
     2521 ctccaattcc aacaacgaga cctcccgcta aataccccca gtcttttcct cgaacagaaa
     2581 caccataatc ttctattctt tttccttcga ccttactagc aagaaataaa gctagtaaaa
     2641 aaacagctaa atgtccgacc gtaccggcaa ttgctccaat ccattcacta tttccaataa
     2701 acatatccgc aagtgcatta ttgacaagaa ttcctaaaac aaaaccaact aagactatta
     2761 aaacagatcc aatcaaccga ataatcctga tcatctttca tccctccttt atattaagtt
     2821 taacaaccct tttctttctt gtctcgtgaa aactatgtaa atgttctcca tcatagagag
     2881 aatagatttt tacgaactaa gaatatctac agcaacctta cgaaaatgaa agactgtctc
     2941 cctaattctt ctgttagact gtttttaata aggaaaggat gggaaatttt ttgaaaaaac
     3001 aactactaat tatgtgcctt tcagcatgtg caattttact attctcaggt tgtagcaagt
     3061 ccaatgaaac gattgcaacg tttaaaggtg gaacacttac agttcaggat ttttatgata
     3121 acccaaaagt taaatcaaat aatcaaacac tattaaaaaa aatgattgtc ttcaaagcat
     3181 ttgatgatgt atacggaaaa gagatttctt caaaggaagt aacaaaagaa tataatgaac
     3241 aaatcaaaaa actcggtcca aactacaagg aacaattaaa agcagttggc caaacagaag
     3301 agacatataa gaaactcttt aaacagatgc tggctttcca atacggtttg aaagccaatg
     3361 taaaattaac agataaagat ctagacactg cttggaaaga attctatcca gaagttagta
     3421 cacagatcat tctattttcg actaaagaag aagcagataa agcaaaaaaa gaagcaaatg
     3481 aaggggaaaa tttctccaag cttgttcaag cttatggaaa aaataaaaca ttaaaggaaa
     3541 ctgatggaaa aatgaatttt gattcaacca atccagagat tcctactgaa gtaaaaaaag
     3601 cagcatttaa actaaaaaac ggggaagttt ctgatatcat tcctgtaaca gatccaacga
     3661 cttatcagca aagctattat ctagtaaaaa tggtaaaaaa acaggataaa ggttctaata
     3721 aagacaaata caaatctgag ctagaaaaaa ttgctaccga agcaaaaact cttgatacgg
     3781 aattcatgga taaaacaatc cgtaaagtga tgaaaaaaga caatgtaact attaaagatc
     3841 cttatgtaaa aaatatattc aaataaacaa atttgaccca attggggatg tggcaaaatt
     3901 tgagaagtga tttttggaaa ttccttctta tttttgaggc aaaccacttt tgccacaact
     3961 tcatcggggc ctttttttat ttgtaccaaa acaaatcact ggattt
//
LOCUS       DACDKL010000195         3985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDKL010000195 DACDKL010000000
VERSION     DACDKL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.197"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(94..1599)
                     /locus_tag="IV329_002431"
     CDS             complement(94..1599)
                     /locus_tag="IV329_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8941588.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1596..2282)
                     /locus_tag="IV329_002432"
     CDS             complement(1596..2282)
                     /locus_tag="IV329_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8941589.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2475..3896)
                     /gene="gndA"
                     /locus_tag="IV329_002433"
     CDS             complement(2475..3896)
                     /gene="gndA"
                     /locus_tag="IV329_002433"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8941590.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1096 a          848 c          684 g         1357 t
ORIGIN      
        1 ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct tattctttgt
       61 tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct ttgcgatagg
      121 aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt
      181 atagttttca accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct
      241 agctcttgct ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg
      301 tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga
      361 gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat
      421 taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc
      481 taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac
      541 ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa
      601 gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc tggcgcttaa
      661 tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc
      721 ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg
      781 ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc
      841 caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat
      901 agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa
      961 acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag
     1021 cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt
     1081 ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt ttccttctag
     1141 tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt
     1201 tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag
     1261 ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag tatgatctac
     1321 gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag
     1381 ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt
     1441 tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt
     1501 aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc
     1561 tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt
     1621 ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa
     1681 cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt
     1741 agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg
     1801 tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt
     1861 tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca
     1921 atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt
     1981 ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct
     2041 cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct
     2101 aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc attatttaat
     2161 gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc
     2221 aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc
     2281 atttattatt ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat
     2341 aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa
     2401 gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa
     2461 cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg
     2521 acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc
     2581 agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt
     2641 acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg
     2701 atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca
     2761 tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg
     2821 atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat
     2881 ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat
     2941 tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt
     3001 acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga
     3061 agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca
     3121 gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc
     3181 agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta
     3241 aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat
     3301 tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt
     3361 ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc ttttgcagaa
     3421 atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt
     3481 gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg
     3541 ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg
     3601 tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt tgcatctgtt
     3661 gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat
     3721 tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt
     3781 gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag
     3841 gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga
     3901 actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca
     3961 ttgcaaacta aagaacaaca tattt
//
LOCUS       DACDKL010000196         3944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDKL010000196 DACDKL010000000
VERSION     DACDKL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.198"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..618
                     /locus_tag="IV329_002434"
     CDS             <1..618
                     /locus_tag="IV329_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8941591.1"
                     /translation="KVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGR
                     FEKDKRPLDMISLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKD
                     IWELYCLSDVFVNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSD
                     DEILTFLKDGRFTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            608..841
                     /locus_tag="IV329_002435"
     CDS             608..841
                     /locus_tag="IV329_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941592.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            1040..1495
                     /locus_tag="IV329_002436"
     CDS             1040..1495
                     /locus_tag="IV329_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP8941593.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            1509..2282
                     /locus_tag="IV329_002437"
     CDS             1509..2282
                     /locus_tag="IV329_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8941594.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            2269..3399
                     /locus_tag="IV329_002438"
     CDS             2269..3399
                     /locus_tag="IV329_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8941595.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
BASE COUNT         1330 a          548 c          841 g         1225 t
ORIGIN      
        1 aaagtgaaac ttgctcctgt ttgtgtggat tttgaacagt tacataagaa ttacgaagca
       61 atagatttta ctactctcag agaaaaatgg aatattcctc gcaatgttcg tgttatttta
      121 tatgtaggac ggtttgaaaa ggataaaaga ccgttagata tgattagttt atttaataaa
      181 cttgatgagc ataattattt gctgatggta ggaactggtg ctttactcga gcaaacgaag
      241 cagcagataa atttacttgg actgcagaat agagtaaaaa taattgagcg tattcctaac
      301 aaagatattt gggagttata ttgcttatcg gatgtatttg taaatctaaa ctatactgaa
      361 atttggggta tggttttgct agaggcaatg tactatcgcc tcgggattat tgctgttaat
      421 gcacctggcc cacagaatat tattttggat aatagatatg gaatgttggc taaatctgac
      481 gatgaaattt tgacattctt aaaagacgga cgatttacca acgatatcat agatgcagca
      541 tatcagcgtg taactacaga atttagatgg tcaaagtcgg gagaaattat gcttagcatg
      601 ttggaaggtg ataaatgata tggaagtaac gaaattaatg gcattgagga atcgatatgc
      661 tttaaacata gttgataatt gcacgagaaa aatagccaaa attcttggtt gttgtattgg
      721 taaaggtgca cagataggga actctgttga atttgttcat aattctgtag gcactgtgat
      781 tcattctgac acaatccttg aagatggtgt taaagtctat cagaatgtga cgtgtggttg
      841 agcagatagt aatgaacgta tcatcattcg agtaaaaaag aacgctgttt tatgtgtggg
      901 tgctaaaatt ctttgtaaat ctaacacaga attatgcgtt ggggaaggtg ctgtagttgc
      961 tgcaaataca gtggtattga ggtctattcc accctacgaa gtgtgggggg tacctgctaa
     1021 aaagatgggg agataaaaca tgaatgcata taaattatac aaagtagaac gatggtttta
     1081 cttacacaaa atggagtttt ttgccaagat tgtaaaggca ctaatttatg tacttcataa
     1141 tagctcaatt tcatatcaaa cagaaattgg agataattgt aagttcttat acggtggcat
     1201 aggttgtgta attggaaaag atacaaaaat aggtaatcac gttataatcg gaacgaatgt
     1261 actgactggt ggtagatcca ataagcctgg tatgcctaca attggaaaca atgtatatat
     1321 cgctactggc gcaaaaattt taggtaatat cacaataggg gacaatgtaa tagtaggggc
     1381 taatgctgtg gtcattaatg atgttgaacc taattgttcg gtaggaggcg ttccggctag
     1441 agtattgaaa aaagatattg atgtaaatga ttattgtagt ttgaatcgtt actaaaagga
     1501 gagattgtat gttgtatttt gttattcctt taatgagtgc gaaggcagca aacaattggg
     1561 agatggttag taccttattt aaccgaacat tatggtcgtg ttataatcag actgatcctg
     1621 atttcaaaat cattgttgct tgccatgaaa ttcctaaact cacgcatgaa tatgatgaca
     1681 gagttgaatt tattcaagtt agcgaaaagg atgcccctat tcccacaaat caagacgaaa
     1741 aaatgattga taagggttat aaaacacatg ctctcgcaat gcgtttgcga gagttgggcg
     1801 gcggtttcgc tatgatggtt gacgcagatg atcttgtaag ttctcatttg gcggaatatg
     1861 tgaattccca tccccaggaa aatggatttt atgtcaaaac gggatacgtg tactttgttg
     1921 gagacgatta tatgaaggtt ctaccaaagt tctcatcagg aagtgcgtgt atagtaaaat
     1981 atgcagtgga agacctgcct gaggcatatc cggaagttat gacagcaaat tgtgatgata
     2041 atccatggat cattagaaaa cgacatgggg gcgttgttcc agcatgtgaa gagcaagggc
     2101 gtccattaaa acctcttcca ttcaaagcag cagtatatgt attaggaaca ggggacaatc
     2161 actcgcttta tggtaaaacg acaaaatatc agacgaaaat gcgagagata ctggaaatgt
     2221 ttatagcaaa acgtaaaatt aaaggaaaac tgaaagcaga attttctgtt gattggttat
     2281 aaggtgagta ttatgaaggt attgatgtgt gaaagccaaa tgccagagaa ttggtattcg
     2341 tttgatcttt ctaaagaact taatcaatat gttgatttaa cattgctgtg taagcgtgat
     2401 gttcctcctt tagatcctaa aattaagagc aaacatattt tgtatcataa aagtacaaat
     2461 aaagtgtttt cggcacttgt ttttctgaaa agcttgttct taatttggaa ggaaataaga
     2521 tttggtgatt atgatattgt tcatttgcaa ttgatgaaaa gtctcaaata tgaaggcgga
     2581 ttgtacgttt tatgtaagag acactataaa aaattaatta tgacagttca taatgtgtta
     2641 ccacatgagg ttcgtgaaaa ggatcgccaa gagcacttta atatatattc aaattgtgat
     2701 ggtttcattg ttcacaatcg agtaacagaa cagcatttat atgctgaatt cccagaatta
     2761 aatgcaaagt catatgtttg tccacatgct atctatggag atgttaaaca tatatctaga
     2821 gcgtatgata aagataagtt tgaatttatc ttgtttggac aaattcgtcc atataaggga
     2881 attgatgtac tgcttaatgc aatcacagaa cttccaatgg atgtaagaaa gaaagttcat
     2941 tttaccatag caggtcaaca acatcctaaa caggatagta ctgactataa aaaaatgatt
     3001 gaagatttaa aggtttcaga ttgtgttcta tataagccag agagaatttc tgatgaagaa
     3061 atggacagta tgttcagaaa tgcagatgcc gcaatatttc catataaaga aatctatgga
     3121 agtggagcgc ttcttatggc atacacttat tgcttaccaa ctattgttag taatgtacct
     3181 gcttttatgg aagaaaccga ggaaggaaag atagggttac ttttcgaaaa tttgaattct
     3241 aagagcctag cagacacaat tgtaaagtat gtgaacctta gcccagctga gaaaagcaaa
     3301 atagaggatg atatatatga gcttgtaaac cgcaagtata cctgggcggc atctgcaaaa
     3361 ctcacatacg aggcatacaa acaaattttg aatgagtagt aaaacaatta gaaaggaata
     3421 ataattaaca tgcagattag taaatggaaa atcatacagt ttctaatggt ctttctaatg
     3481 attatctcat caagcatatt gaagcctttg ggcggctggt tgggagaaac gatgaacgtt
     3541 agacttgtga cagtcattct agcaatagta tgtttgttaa tagcctcatt accagcattt
     3601 agaaaaaata gatatttaaa tttctgcact ttactgattt gtgttgttat aatagcagct
     3661 tacatctact cagataagac aatatgggtt aaatctaggg aaagtatgag tttatactat
     3721 gtattattgg cgtatccatt gttctgtact ttaattaacc aagcgtggaa tttggattca
     3781 atgctaaata cgatatgtgg tgtaacattt ctttcataca ttcttagaac atctatttca
     3841 cttattcaga aattttcagg agtacttctc tatgaaaaca tatatcgtga gtgtgccccg
     3901 gaaaattggt atcgtggagg ctttttaaga attaatccac catg
//
LOCUS       DACDKL010000197         3871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDKL010000197 DACDKL010000000
VERSION     DACDKL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.199"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..739
                     /locus_tag="IV329_002439"
     CDS             71..739
                     /locus_tag="IV329_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8941596.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            742..1785
                     /locus_tag="IV329_002440"
     CDS             742..1785
                     /locus_tag="IV329_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8941597.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(2022..2903)
                     /locus_tag="IV329_002441"
     CDS             complement(2022..2903)
                     /locus_tag="IV329_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8941598.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            3062..3790
                     /locus_tag="IV329_002442"
     CDS             3062..3790
                     /locus_tag="IV329_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8941599.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
BASE COUNT         1200 a          636 c          816 g         1219 t
ORIGIN      
        1 aaataaatgt taactgaaga tgaactacaa gaaaaaatct agaaattttt caaaaaacag
       61 agggagatca atgaatcgta ttttagtagt agaagatgat ccgcaattac agttgcttta
      121 ccgttctgtg ttggaacgag cgggcttttc agtcatcacc gcattgaatg gagaggatgc
      181 gctgaaaaaa ctagaagcga cacgtataga attaatcatt acagatatca tgatgccgaa
      241 aatggatggc tatgacttgc tagactcatt acgtagcacc agatttgaca cacctgttct
      301 aatgattacg gcaaaggccg attttgagga taagaaacgc gggttcaaat taggagcaga
      361 tgattatatg acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg aagcattgct
      421 tcgacgttca aagatcaatt acagcagtag cttgataatc ggacaaacaa aattagagct
      481 agatgcttat acagtgttac aagatcagca ttcgttgatt ctgcctcaaa aagaatttct
      541 gcttctgtat aaattactgt cttatcccaa caagatcttc actcgccaac agttgatgga
      601 tgatatttgg ggattagata cgaatacaga agaacgaacg atcgatgtac atatcaaacg
      661 tttaaggacc cgttttgaaa agacggaaga ttttcaaatc atcacagtac ggggactagg
      721 ctataaggcg gttatttaaa gatgaaaaaa aagctatttt cacaattatg gatctatttt
      781 gctagtattg tcttcatttc gatcttagtg acactgctgt gttttctcgg ttttattttc
      841 ttattgtctc gacattctat ctcgcctgct gaatcggctg gaaaaccaac gttatacccg
      901 ttattcgtct ttgcggggtt cagtatggtc gtgggtacag gaatatcgat ttttgttggg
      961 cggcgtatcc tgcatccaat cagtgcgcta gggacaaata tgagtctggt agcaacggga
     1021 gatttttcga ttcgaatgga tgaacagcag aaagtagcag aagtacagca actgtataaa
     1081 gatttcaacg ttatggtcca agaactcaat agtatcgaga cactgaggaa tgactttgtg
     1141 tccagtgtat cccatgaatt caaaacacca ctagcaacaa tccaaggata tgttcaattg
     1201 ttacaagccc caaatctctc tgacgaagaa cgccaaatat ttttatatcg gattatcgaa
     1261 agtatcacgc agctttcaca attaacagag aacatcttaa aattgaacaa actggaaaac
     1321 cagcgtatcc aactggaaaa gaaagagtat cgattagatg aacaaatcag agaagtaatc
     1381 gtttttttgc agcctaaatg ggagaaagaa cagctagaat tggatatcga acttgctgca
     1441 gtaaattaca caggcaatga agagtttttg taccaagtgt ggctgaatat catggataac
     1501 gccataaagt acaatcaaat caatggacag attcatatca aactatttga gacagccaca
     1561 gagattggac tggaagtgac cgattcaggt gtaggaatga atgaagagac cagagatcga
     1621 atgtttgaaa aattttatca aggagataca agtcgtcaaa tttctgggaa tgggctagga
     1681 ctttcactgg ttaaaaaaat tctagaactg catgatggac gtattgatta tagcagtatc
     1741 gaaggagtag gaacgacagc aatgatacgg ttaagtaaac aataggaaaa agaataaaac
     1801 ccctaaaaat tctattgaag gtttttaggg gtttttagtc gtttttttgt gattagggaa
     1861 atgttcttac ggttcttttg gcataaaaat cgctgctttc ttattgaact acgagaaatg
     1921 ttagaggtgt cattgaaaca aggacatgat gcaagaaaga gagcgaaagt catgcgtcgc
     1981 tctctttttt tgggtggata gatggacaga aaatgtaggg cttagttttc ttcttgattt
     2041 aaatactccc aaaatgtatc tgaagccaaa gaatgctcac cgactttacg agtaaatgca
     2101 agataactct ctgttgttgg agaaataggg agaatcacag tattttcttt taatagattc
     2161 ttgactgttt tttccataac aatagcaatc cccatgtcat ttgcaatcat attgatgatc
     2221 aagtcgattc ttgctccttt ataagagatt tttggttcaa atccagcttc gcgacacaga
     2281 tctagtacag gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt ctcctttttc
     2341 agttcgctta gatccaattt ctttttacta gctaaaggat gatttttagg tagaactgct
     2401 acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc ctggaaattt
     2461 ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa ttcgacactt
     2521 tccacttctt tcaactggac tgtaaattca ggatggcgtt tcaaaaactc ggtgattttg
     2581 gaaaaacctt tataattgat cattgtcggt atcgttaaga tatttaatga aagattgttg
     2641 tctttcgtat aggtttcaag cacttcttgc atagcatggt attgttctac gacatttttt
     2701 acatatggga gcaataagct tccagcttcg gtcaattcga tttttctatg ggcgcgttcg
     2761 aataatttga cgtctaattc tgtttctaac gaaagaatct gtttagaaat atttccttgg
     2821 gtggtgaatt gtcgatcagc agttttggtg aaattcattg tttcggctaa atcaagaaaa
     2881 acttcgaatt tatggatatc catcatattc ctcctactta ttccaataaa gaatacttta
     2941 acacaaaaaa tgaattggtg taatcttctc tgtctgttac aatctttctt gcagcgaaag
     3001 caataattac agaaattttt ctgatttttt tcgcttcgta gaaaaagaga ggagtttttt
     3061 gatgccaact ggagttttga tcaatgtagg ttccgttttg cttggcggac taattggcgg
     3121 attagtagga aataaattaa gtgaacattt taaagcacag ctgacgatgg tttttggtgt
     3181 gtgttcgatg gggatgggaa tctattccat tgcgccgatg aaaaatatgc cggctgttat
     3241 ttttgcttta gtgataggaa cagcgattgg tctgattgtt catttaggta atgggataaa
     3301 taagggggct gcgctgatgc aagtacctat ttctaaaatc ttccctagtg aaaagcttgg
     3361 tatgacccac gatgaattta tcagcacatt agtgacggtc attgttctat tttgtgcaag
     3421 tggtacgggg atctatggaa gtttagattc tgggatgaca ggagacagta cgattttgat
     3481 ttctaaatct gtccttgatt ttttcacagc agctattttt gcgtgtaatt tagggtatgt
     3541 cgtttctgtg atagcaattc cccaattcat tattttttat attttgttct tattggcaaa
     3601 atttatttat ccattgacta caccagacat gatcttagat tttaaagctt gcggcggatt
     3661 cttgatggtc gctaccggtt ttagaatgat caacgtgaaa atgttcccag tagctgacat
     3721 gatacctgca atgatcgtta tcatgccact gagctggatg tggacaaatt ggattatgcc
     3781 actgttataa taaaaatcaa agagaagcca ttttttgctg actaaaaagg atcaaatctt
     3841 ttagtcagtt tttttgtatt tgacaaactg a
//
LOCUS       DACDKL010000198         3852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDKL010000198 DACDKL010000000
VERSION     DACDKL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.200"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..1085
                     /locus_tag="IV329_002443"
     CDS             117..1085
                     /locus_tag="IV329_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8941600.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1096..1911
                     /locus_tag="IV329_002444"
     CDS             1096..1911
                     /locus_tag="IV329_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8941601.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1979..2689
                     /locus_tag="IV329_002445"
     CDS             1979..2689
                     /locus_tag="IV329_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8941602.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2725..>3852
                     /locus_tag="IV329_002446"
     CDS             2725..>3852
                     /locus_tag="IV329_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8941603.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1276 a          565 c          856 g         1155 t
ORIGIN      
        1 acaatgcaat tatgtcacat gacgttatat gacgttatgt gatatcttta catttaatca
       61 gttactttta ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg
      121 atgtagcaaa aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt
      181 atttggtggc ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag
      241 ccgaagcatc gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc
      301 caaagcaatt agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag
      361 aaacggtaaa agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc
      421 ataataaaac tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag
      481 atgatgcaaa aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg
      541 tcaatgttac cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg
      601 ataccgtaat tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata
      661 agtacaaaga tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt
      721 atggtttttc aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt
      781 ctggagaatt cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct
      841 tgatgagcat tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat
      901 atgcagaaag agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg
      961 aagtttctga attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag
     1021 ctttagcaga tgtacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt
     1081 actaggagga ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa
     1141 aatctgggaa ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa
     1201 ctgggacatg aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt
     1261 ttatcagcaa taaaagaaat aggactggac aacagtcact ctcgctttga aaaaggcgag
     1321 aatggctgtg ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg
     1381 ggaggtgtta ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac
     1441 cagtttgaat tgattcatat gggattatat agccatgtca atcatctatt agaagagtta
     1501 caaaatgttt ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt
     1561 cagcgggcaa ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa
     1621 gaagtaaagc agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc
     1681 cgaggagaaa aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg
     1741 ctagaagaac cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt
     1801 tattttctcg aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg
     1861 aagtcttgtc tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt
     1921 aaattcctgc atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat
     1981 gctggataca aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa
     2041 aatcaataca ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga
     2101 aagttatgag gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg
     2161 gtacctactt aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa
     2221 aattgaatat attatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag
     2281 cattgttttg gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat
     2341 cgctcaaggt gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat
     2401 tatgttagaa aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct
     2461 gactgattct ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga
     2521 aactgcactt gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt
     2581 tgggacgccc tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca
     2641 tgtaggtaat caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttg
     2701 gatttcaagg agtagatttt atttatgaag caaatattgc ggtcaaaaat gatttttgat
     2761 ggagagcagc cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg
     2821 gaaaacaact ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat
     2881 gatcaattcg ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct
     2941 atgaatgctg gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt
     3001 aaagctgtaa tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct
     3061 gccctgtgga aaactgaccc atcagaagag ctattgaatg aaataaacca ctttggtcca
     3121 atcacagcga tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact
     3181 tatggtttta cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg
     3241 ctagcaaaca aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca
     3301 aaaggtgtta tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata
     3361 cctcagagga aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat
     3421 gaacttctat atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg
     3481 aagatcatgg tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag
     3541 tcaggagtag cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc
     3601 aatgaagtag agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct
     3661 gcgtcaattc ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt
     3721 gatttggaaa tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca
     3781 gaggtatatc cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact
     3841 gttataaaaa at
//
LOCUS       DACDKL010000199         3850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDKL010000199 DACDKL010000000
VERSION     DACDKL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.201"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..624
                     /locus_tag="IV329_002447"
     CDS             169..624
                     /locus_tag="IV329_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941604.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            743..1801
                     /locus_tag="IV329_002448"
     CDS             743..1801
                     /locus_tag="IV329_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8941605.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            1843..3792
                     /locus_tag="IV329_002449"
     CDS             1843..3792
                     /locus_tag="IV329_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8941606.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
BASE COUNT         1425 a          562 c          774 g         1089 t
ORIGIN      
        1 tcttttatga tgactgaaat agctaaaata aatatcttgt tgaataattg ctgatacttt
       61 aagggatatc taaatttgct gattgattta tttatgttat tatgatatta taataacata
      121 aatgtgttac gcccgaacct cattttaaaa aaatgaaggg gtgagacaat gaatgctatt
      181 aattggcatg aatttgttaa ctatatggag agcttactag atgatctaaa aagttttagt
      241 attgctgatg cagtaaagca tgggttacca gttagtacaa cgatgaaatt acgaggattg
      301 aaaattccag atgaaattat taagctgaga actaaaacaa ttattgaaac cgcaaaagtc
      361 gttgacaata tgaaccatga actaaaaggc aagaaaacaa cttacgagaa agattttgaa
      421 cgttttttaa aatctgttat tggaaaatat ctaggtaaaa aatctttcag tgaattgaga
      481 gatgaagaga ttgtacctac tccaatggtt agcaaaatcc gaaaaaacaa agctgagggg
      541 aattactatc ttggtacaat agcaaaaatc attgattatt tggaaaataa agagcaaaag
      601 aaaggaacga agaaaggaag ataatttttg tgttaaatat taatggggaa gaatataccg
      661 aaatgattca aaatcccgaa cctagaatta ccgaaaaaac gtctgattgt ttgctctttt
      721 atgaaagggg gcagacaaac tgatggaaaa aaacgtcaat tcaacatcta ccaaagatca
      781 atacagtatt attggagggt tgcttggtga taaggataag agaacacgtc acggattacg
      841 cattatgctt gcaacttttc taggagtgct ttttttatta acaggcttgg gtagctacat
      901 ttatcaatta ggaaactata attattatct cgtgaacaat acaacaccta tagggtcttc
      961 attggagttt gctcgatcag gagcagaaat cactatttca gatgtgtgga cagatcaaaa
     1021 tagagatgta acagtagtaa aacttggtta ctcccaaaac gctagaaagt tgttatctac
     1081 gcaaggaaaa aactataact tatatatggc aacaaaagaa aaagatcgtc ctaagaagct
     1141 tgaaatgagc tatgggatgt taagtactga tggggatgct ttcttattcc taaaaggtaa
     1201 attagaggaa agagcttatc aagtttttat tgcaaaccaa gtatcactaa tttctggtga
     1261 tgatacagct gacgatgata ccaaagtcaa tgtgaatgaa gcagattcag ttacaaaagc
     1321 actttctcaa tattctatga atgatgttaa taatgaagga gtaatttctt ttgggaaaaa
     1381 agcaaaacct gttaatactc ctgataatat caatttcaga ataaatccat attcagattc
     1441 aactaatgtc tatcaaggaa gctttcttac caaagatggc gatattgatt attcaaaagt
     1501 tgtagcagtt acaagtaaga ataatgttgt aaataagcta gaagatcagt taaatgcttc
     1561 aaatactaac atcaagaaaa ttgaagccac aaaagctgag tatctggaac gtttacaaca
     1621 aaatcctgac gataaagatt cgcaagaaag tattcgtcaa ctagacagtc gaatggagac
     1681 cgaaattaaa gcaatcaatg atacacaaaa attgattgat caatacaaaa atgctgactt
     1741 taacaaagat tcttttggag acatgaagac gacatttaag tacctaactt tgactgacta
     1801 gaagtaaatt attcttattt tagaggaagt gaacacaaac agatggaaaa tagtaccgag
     1861 ttttttaaga atattcaaac cgaatttaat catgctctca caccaatgaa accaccaaaa
     1921 cataagttag tgggacgtga tgacgaactc catgagttgt cactgatctt gaatagaccg
     1981 gaaacaccgc aagctatctt gttaggtgaa gccggaacag gtaaaacagc acttgcaagt
     2041 gaatgggctg aaagacagcg acaagatgga aagaaagtac aagtatataa attatcaatc
     2101 ggacaattgc gtgttggggg agatgatgtc ctccaaacac gtttagaaac attaattcca
     2161 agattgaaag aatatgagcg aattttaaaa gctgaggaca aagataatga atttgtcctc
     2221 ttcattgacg agtttcataa agttgttagc tcttttggga gtggcaataa aattgggggt
     2281 gattcgctaa aagaaccgtt agcaaatgaa tcacttcaaa tgattaccgc tactacaaga
     2341 aaagaatatg aaaactatat caaaagagat gatgccttag ggagacggct acaaccgtta
     2401 gaattgaatg aactaaaaag cgatgttgtc gtcgatattt tgagacaatg gctaaataca
     2461 tcctctgtta aagatgatcc tcaggcttct tttagattat cgcaaaggat tcctgatgaa
     2521 ttacttcaag aaatcgtaca agcaaacaaa ctttatcgtg aggatttgtc cgaaccggct
     2581 aaatcaattg ctgtaatgga ggatttgatt gcaaaagatc gagtagacgg aaatgcttat
     2641 aatcgagaaa gtctattgca aatatttggt aagtataata ttcagctaga ttctgatttt
     2701 gatatggaaa aacttcaaga ggaattaaag tccataaaag gacaaccgtt agcagtttat
     2761 actataacaa tgttgtttaa acgtatgaag cttggatttg atcgttttaa aggtcgaaag
     2821 caaccaaaag caactgctct gtttacagga tcaacagggg tcggtaaaac tgcaatggct
     2881 aaagccgtgg caagaggact ttatggcgat gaaaataaag ttctcttagt taatatgccg
     2941 gactacaaaa ccaaagatga tgcaatgcgt tttagacgac ttataggtac tcatgtaagg
     3001 catgatcctt ctgcgattat tgtgtgtgat gaatatgaga aagctggtgt agaaacactc
     3061 gatgcctttt tatcaatttt agatgaaggt aggataagtt ttactgaaag aggatgggat
     3121 gatcgagaag ttcacgacac cgtttcattg tcaaatacaa ttttatttgc gacaacaaat
     3181 gcaggtgcgg agatgttcga ttcgttaaac cgatatagct cactatctcg tgttacaaac
     3241 aaggaaaatt ctaatgaata cagcactgaa ctaaaaagag aataccgaaa agtcgcatca
     3301 gggatcaaac aagccttgat tggtgaaggt ctaaagcctg aagttattaa ccgatttgga
     3361 cgaatagttc cattttgggc tttatccgaa caaactcttc tggaaattgc ttataatgaa
     3421 atgttgaaga gtgctgaatt agtacgaaaa catttaaata tagatttacg tcttccacct
     3481 aggaaagaaa gagtatatgg tcaaaatttt tatgcagacg atctctccat gcacttagtt
     3541 tttgacatgg cttcacaaga ggattctaaa tcagggggtg caaggaatat cttgcattta
     3601 gttgaatctg agtttgaaga agcgattact gaagcaataa ttacttatcc aaacgcaaaa
     3661 atattcgatg ttaagttgag tgaagccgga gcgtttgaag gaaaaggtga ttctaatgct
     3721 gaaattattg caaagccttc cgttgctgaa agtaaagaaa acaagattgt attttcgatt
     3781 ggaggggcgt agctatgaaa ttcaagaaat atttactaca aaaccttaga ataaaacgat
     3841 gggtcgtaag
//
LOCUS       DACDKL010000200         3771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDKL010000200 DACDKL010000000
VERSION     DACDKL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.202"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IV329_002450"
     CDS             complement(27..725)
                     /locus_tag="IV329_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8941607.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IV329_002451"
     CDS             complement(718..2070)
                     /locus_tag="IV329_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8941608.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IV329_002452"
     CDS             complement(2293..3693)
                     /locus_tag="IV329_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8941609.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
BASE COUNT         1215 a          784 c          650 g         1122 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca g
//
LOCUS       DACDKL010000201         3766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDKL010000201 DACDKL010000000
VERSION     DACDKL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.203"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IV329_002453"
     CDS             202..1077
                     /locus_tag="IV329_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941610.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IV329_002454"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IV329_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IV329_002455"
     CDS             complement(1642..2973)
                     /locus_tag="IV329_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8941611.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..>3766)
                     /locus_tag="IV329_002456"
     CDS             complement(3122..>3766)
                     /locus_tag="IV329_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8941612.1"
                     /translation="KIGEKLPTGDELAVIYHTSKLTVKKGVDLLVSEGVLRSRSGYGT
                     EVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTFSIELPSKKVADKLSISVKDYVYN
                     IVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKSVYAYITNELGLEIKTSHIRIKGD
                     TANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEYSVSRHLYQDFVFEAVFVEK"
BASE COUNT         1302 a          693 c          569 g         1202 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aatttt
//
LOCUS       DACDKL010000202         3757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDKL010000202 DACDKL010000000
VERSION     DACDKL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.204"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(180..473)
                     /gene="rpmA"
                     /locus_tag="IV329_002457"
     CDS             complement(180..473)
                     /gene="rpmA"
                     /locus_tag="IV329_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8941613.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(486..830)
                     /locus_tag="IV329_002458"
     CDS             complement(486..830)
                     /locus_tag="IV329_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8941614.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(857..1165)
                     /gene="rplU"
                     /locus_tag="IV329_002459"
     CDS             complement(857..1165)
                     /gene="rplU"
                     /locus_tag="IV329_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8941615.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            1447..2088
                     /locus_tag="IV329_002460"
     CDS             1447..2088
                     /locus_tag="IV329_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8941616.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2150..>3757)
                     /locus_tag="IV329_002461"
     CDS             complement(2150..>3757)
                     /locus_tag="IV329_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8941617.1"
                     /translation="LKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGVSSGADAAA
                     GLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATFLAGLFLLA
                     AGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEKLSSYQTIG
                     FPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPVATVGKIPQ
                     TLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKPLDSNQELM
                     AQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSMLVFAPVMSQ
                     IPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDLSIAIVIGV
                     IGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMSSEKLKNKL
                     EELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEVEKQLSHLW
                     EHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1160 a          833 c          665 g         1099 t
ORIGIN      
        1 atagcttcca tcaattgaaa acgatagtta tcaaaacatc ctgattccaa attggcaaaa
       61 aaatattaag tgtaaaaaga caaagtaaac ttcgtagcct ccactacaac ttttattttt
      121 ataaacaaaa aaggctgtga caaaagtctt gtcacaacct ctttaatcat aagattcaat
      181 taagcttctt gagctactgg ataaacagac acttgttttt tgttacggcc tttacgttcg
      241 aaacgtacta cgccatcaac ttttgcaaat aaagtgtcgt ctccaccgat acccacgttt
      301 acacctggat agatttttgt accgcgttga cggtaaagaa ttgatccacc tgtaactgtt
      361 tgtccatcag cacgtttagc acctagacgt tttgattctg agtcacggcc gttagatgta
      421 gaaccgccac cttttttgtg ggcaaataat tgtagattca tagataacaa catggtctgc
      481 acctcctatt tatcattaat tgttttaatt tggatatatt ctgagttttc agcttctatc
      541 gattgtaatc ctaataaaag attttctaat agaatctcag caatattgtt ttgttcttgt
      601 gtcattcctg aagtgacttc ggtataaaga taaccgcctt cttcttcatt catttcaaca
      661 atcggctcaa aaccggctaa tgaggcaatc ccgttgacag tgctgatcgc taacgcagaa
      721 acacccgcac aaacgatatc acttccataa ggaccagcat cagcatgccc ggtcaaagtg
      781 aatgaaacga tctgacctgc gtcatttcgc ttaaatgttc ctttgatcat atataggcct
      841 tctttctcaa acgctattaa gcgttgatcg cgtcgataac cacttttgta tatggttgac
      901 gatgaccttg tttacggtgt gaatgttttt taggtttgta tttgaaagta acgactttct
      961 tttgtttgcc gtgtttttca acagtacctt caacagttgc acctgcaaca gttggagcgc
     1021 ctacttttgt agattctccg cctactaaga taacttcgtc aaaaacaact ttttctcctg
     1081 cttcaacgtc aagtttttca acatagattg cttgtcccac ttcaactttt acttgtttac
     1141 caccagtttt aataattgcg tacatgctat aaagcacctc cttctgatat acttagactc
     1201 gccatccgaa gtgacagcat atgctgtgct tattacttgt tctgagcggt tgtagctgtg
     1261 gagtccacaa ttacaacagt actagcttac cagaacagca accgttagtc aactaaattt
     1321 aaaatcaaaa agtaaaacaa aaattcaagc aatacttctg cttttctatc ctcacagttc
     1381 taccaaataa aatagtcgca ttgtgtttct tttattctta taataaaaga aaaaggagga
     1441 gcataaatgg aatacccaaa agaactgata caagaagctt ctttaagaat gaacggccgt
     1501 cctgtagaag gatttatcgc tgggcaagga cctcttcatc ctaaactgat gctcgtggga
     1561 gaagccccag ggaaaacaga gattgatacg catgttcctt ttagcggtca agctggaaaa
     1621 gaattgatgc aggcactttc ttcaactggc ttaacacgag aagaggtgta tatcaccagt
     1681 gctgtaagga gccgcccgta tcgcgtgact catcggatca ataaacggac acaacagtca
     1741 gaaactgttt atccaaatcg gacacctact cgttccgaag tatttgcgca tgcaccaata
     1801 ttggattacg aactgatgaa ggtccagcca aaattaattg cgacacttgg aaacattggt
     1861 cttcaacgtt tacttggcaa agaatatacg gttaccaaaa atcatggtca actctataca
     1921 gggcctgtct tacaattaac agaacaaaag gatgcctacg aatggtcaga aaaaaactat
     1981 cgaattcttc cactttttca tccagcagct attttctaca atcgctccct tgtacctgtt
     2041 atccatcaag actggcaagt actagcttca cttttacagc aatattaaaa agaacagaca
     2101 agaccataaa atcttcagtt tacagtcttg tctgttcttt ttcattgttt taatggctaa
     2161 ttgcagtata tttcgcatgt aaagctgtca acgcatgagc aactgtttga tgttgatgcg
     2221 tgaatgtatg ttcccacaag tgagaaagtt gtttttctac ttctggctgc aaggaagtat
     2281 agataatcgt ttgtcctctt tgctctgctt tttcttgaaa ttcatcaaaa actgcacgtg
     2341 ccgtcaaatc aatacttggt acgcctcgca tggagaaaat gacaccatcg tgatgattta
     2401 gttcttccaa tttatttttg agtttttctg aagacataaa aaacagtgga ccactaatat
     2461 acactactgt ccagttattc agcttttccg tttctggcag ccccattctt tgccaatcga
     2521 tcgtttctaa tgaaatcgtg atagcagcgc ttttgacaac aaaaaataca cagccgccga
     2581 tcaccccgat cacgatagca atacttaaat caaagataac tgtacaaatc atcgtaagga
     2641 taaacaatag aactgctgac caatatttat tagtaaatag ctccttgatc gtttcccatt
     2701 cattcatacg ccaagccgtc acaatcaaaa cacctgcaag tgctggcatt ggaatctgtg
     2761 acatcactgg cgcaaaaaca agcatggaga gcagtaagac aactgcatgt atcataccag
     2821 ctaatctagt ttgtgcacca gatttgatgg ccacgcttgt acgggcgatg gccgcagtag
     2881 caggaatccc gccaaagaaa ggaagcaaca gatttccaat tccttgcgcc attaattctt
     2941 gattgctatc taagggctta tttgccattc ggccagccga agcaccacat agcaagcttt
     3001 caatcattcc taataaagca atgctaatag ctggaactgc cacttgttgc aaggcagaaa
     3061 agctaaaatc tcccatattc aaacgattac tactgatcaa tgtttgtgga atcttaccca
     3121 cagttgctac tggtaaatgg aacaatagca taactgctgt tgccaaaatg atagccagca
     3181 gcgaactagg cattcgtttc gcccattttt ttggatagat aaacatccct aaaatgacta
     3241 gccctcccat gatcaaagag gctgtgttta tcggaaatcc tatcgtttga taactactta
     3301 gtttttcaat aacgtttgta ccttccgagt gaacaccaaa taaattatcg atctgaccta
     3361 atgcaataat gatagcaata cctgaagtaa accccgtaat tacgggtgct gggataaaag
     3421 aagtcagtgt tcctaatcgg aaaagtcctg ctgcaagtaa aaatagaccc gctaaaaaag
     3481 tagcaagcaa tacaccttgc attccctttg ttgccgcaat agacatcaag atagctgcca
     3541 tcgcacctgt cggacctgat atttgataaa aaccgcctga taaaccgcca ataacgatac
     3601 cggcaatgat agccgtaatc aaacctgcgg ccgcatcagc tccgcttgat accccaaaag
     3661 ccaaggctaa aggcaatgct acagcagcta ctgtgatccc agccaaaaga tctttctgaa
     3721 atcttgaaag atcataacct gaaaattctt tttttag
//
LOCUS       DACDKL010000203         3695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDKL010000203 DACDKL010000000
VERSION     DACDKL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.205"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..889)
                     /locus_tag="IV329_002462"
     CDS             complement(<1..889)
                     /locus_tag="IV329_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8941618.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSD"
     gene            complement(1346..1774)
                     /locus_tag="IV329_002463"
     CDS             complement(1346..1774)
                     /locus_tag="IV329_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941619.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1802..2440)
                     /locus_tag="IV329_002464"
     CDS             complement(1802..2440)
                     /locus_tag="IV329_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8941620.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2444..3214)
                     /locus_tag="IV329_002465"
     CDS             complement(2444..3214)
                     /locus_tag="IV329_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8941621.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1109 a          705 c          553 g         1328 t
ORIGIN      
        1 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
       61 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
      121 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
      181 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
      241 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
      301 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
      361 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
      421 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
      481 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
      541 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
      601 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgagaatcga
      661 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
      721 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
      781 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
      841 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
      901 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
      961 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
     1021 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
     1081 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
     1141 aatgttatcg attttgtttt tcttcttccg ctgctgggat attcaaatca gacactttta
     1201 tgttcgattt tccagacatt tttttgttgt ttttattatg atatattttt atacaactaa
     1261 aaatgaatat attaaaacta ccaaaaataa cttttaacgc tgtttcatta tatgtttttt
     1321 ctattttata taaacttttg gatacttaat accaaccggt tggattgtac caacatgaga
     1381 atgtgtcagt tctatttcct gtcgcacttg cataggccca tactcctttt gttgcatttg
     1441 ctgatcgaat agagtttcca actttcgctt ttgcaccgtg acgattatag acacgactat
     1501 agtggttaga atgacctgtt tttctcgtac aagttgtatc actataataa acccatcttc
     1561 cagagctatg attaattgct gttctagaaa tttcccggtc tactggtaaa tttgacttaa
     1621 taatattacc tttagcatca aaatgaatct ctcctggctt taaagatttg taatagattt
     1681 caccgtgaac tgaatttgct tccacttcag atgacatagc gtttcctcca acaaaaccta
     1741 tccctagtgc acaaataata actgcttttt tcataaaacg acatcctttc ctttttgatt
     1801 tttaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc gtgactacga
     1861 taactaacgt accttgggaa gcttgttgcg ataagatttc aactacttta tccttatttc
     1921 ttttatccaa tgacccagtc ggttcatcgg ctaaaatgat tttgctattc ttcaataaga
     1981 ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata ggcgtactta
     2041 gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc ttatctgaaa
     2101 catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt tcatattcaa
     2161 tcaacccaaa atcttgaaaa agaaaagata tggtatttct ttttatcaag cgcttctctc
     2221 tcccagttaa ttgtttcgtc tcttgatcga acaaatagaa ttgtcctgaa ctgaactgat
     2281 cattcaatcc taatatattc aataacgtac ttttacctgc tccactttct ccaacaacag
     2341 tcacaagatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt ttgtttcgaa
     2401 tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct ccattcatca
     2461 gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga ataaaatcaa
     2521 tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata agaaaaagga
     2581 agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct ttatacgttt
     2641 caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg tagacaaaat
     2701 atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt aaaaatatct
     2761 gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca gtctgagtat
     2821 acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc tcaaataacc
     2881 tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct ccgctgagaa
     2941 tatttctttc caaatacata ctgtttttca atgtcagtat ttcaacaacc ggataatgtt
     3001 cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt ataaatagaa
     3061 atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc aggtcatttt
     3121 ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt tctttttcgg
     3181 ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga taattcagat
     3241 ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct gatacattga
     3301 ctaatatact tccgttttta ttcaaagttt catataattc ctgatctgct gctgatgcaa
     3361 aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga taaaatacat
     3421 gatagtcctc accagcgatt tcttttggtg gttcatggga aacagctaac tgtgaagaag
     3481 aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa aacaacaatc
     3541 caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa gcgatttttt
     3601 caactaataa aagagaaatc aaagagatac catatattcc gctgaacaag aaaacgtaca
     3661 tctggaacaa ataaagaaat tgctccttat aatac
//
LOCUS       DACDKL010000204         3636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDKL010000204 DACDKL010000000
VERSION     DACDKL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.206"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            291..2171
                     /locus_tag="IV329_002466"
     CDS             291..2171
                     /locus_tag="IV329_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941622.1"
                     /translation="MDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNL
                     FWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFT
                     TQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMI
                     RNLRIGLVAVSLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQS
                     VSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYT
                     ALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDA
                     EASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGP
                     SSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNP
                     LSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLP
                     ITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGE
                     KFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKK
                     YL"
     gene            complement(2753..3625)
                     /locus_tag="IV329_002467"
     CDS             complement(2753..3625)
                     /locus_tag="IV329_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8941623.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT         1292 a          606 c          711 g         1027 t
ORIGIN      
        1 tatttttacc gatgttttta ttaggagccg tacttgtcat aggatttagt ttattcattt
       61 accagaatac ctatgaatcg atcgaatcga gttatttaac agacaaaaag aatttgctca
      121 ggcaagttaa aactaacgtt gaatggaaaa ttagaacgat tgaatattcg ttttctacgt
      181 atggttcgac caaaaatttc tcggacatat tcaagcatcc gctgacatat acagattact
      241 ccacttattc tgaaatcagg aaagaactaa attttataga aacgattgtt atggatgata
      301 ataattatga tttagtcagt ttggctggta aatggggagt aatcaatgga tcgttgagtc
      361 aactgaccga agaggaagtt gaaaagtatc aacagaagta cataaataac aaaaataatt
      421 tattttggca aaagaaaaac aaaggaatcg aaatggtgat ttctttgcca atgtttgaaa
      481 aagaaaaata tgcattggga attgctacga tagagaaaca tacaatagaa caagttgttg
      541 gtgaaagtgg agaagacata ctttctattg aaaacgaagt tggcaacctt tttactactc
      601 agacagtaaa aaataaagag caaccaactg tattaacgga cgtggatcta gattacaatc
      661 agctgaaagt gataagagaa aaagataaaa cgtatatagt actgaaatca gattataata
      721 attggattta cagattagaa gttgactcta gtgcaattgg cgcaatgatc cgcaatctga
      781 gaatcgggct agttgcagtg tctttaacac tggtaggact cattggtctt ttttcctact
      841 tattttcgga cagatttgcc cgtccgattt ctcaaataca ggaacgtctg aatcttaaat
      901 caaaaggatt cagtgggaaa gaactagatt tggtcgctca atcagtcagt cggatcatag
      961 gagaaaaaga agcgttaagt gcacacttag ttacccaaaa gccacagctg gaaacgctat
     1021 ttgtacttag tttatttaga aatcgagtag aaaggcgaga actagatcag cggttgcagc
     1081 aatttggtta tagttctcaa aatgactgct attatacagc tttggttcaa atcgatctgt
     1141 tagaagatag tcacggtggg gagcgtgatt tgctactttt agcgatcaac aacatgatca
     1201 cggaaattgt gccaaaggaa aatcgaatgc tgccgatcgt tttgaatgaa gaaatgcaag
     1261 ccactattta tcgaatggat aaagatgatg cagaagcaag caaaaaagtc atggagtatt
     1321 gccgactcat tcaaaaacgt gtaaaagaat acttgaattt aactatcagt ataggcatca
     1381 gtaaccggtt cgaaacgctg aatgaaagca agcaatcggt tgatcgggca aaagaagccc
     1441 tgtattatcg tgtaaatact ggtccaagtt caattatttt ttatcaggaa atcgttcctg
     1501 ctgctcatga aaaaacactt attagatatc cgatagaaga acaaaatcga ttgtttgaag
     1561 ccattcgttc tggaaatcag gaagtgaaga atttagtaca tgaattgatt gatgcattgt
     1621 ttgcacaaaa taaaaatcct ttgagtcggg aagtagtttc ggtccgcttg atcaatgaac
     1681 tcgttcaatt aggacagtta ttgggagtag attcaaaaaa ctttgataac atgaaacaaa
     1741 tttatgtaaa agcactgaac aattatcatc ccaaagaact agaaaaactt gttttagaac
     1801 aattagttct gccaatcaca gaatgctctc aagcaatcac tgatcatgaa tttaaaaatt
     1861 tgtcggagaa tattatgcat atcgtctaca cagaatatga tcgtgatctg tctttagatg
     1921 tgatcgctga taggttgcat tataacccta actatttaag taatgtattt aaaaaggaaa
     1981 caggagaaaa atttggtgat tttgttcaaa atcatcgttt agaggtagca aaaaaatggt
     2041 tgaaagagac taacctctcc gtaaaagaga ttgcagaaag attgcaatat cgaaatccgc
     2101 aaaattttat tcgtttcttt aaaaagaagg aatcgattac gccgggagaa tatcgaaaaa
     2161 aatatttgtg aatcaagagg ctgtttttct tgtgatctga ttcaagcaat aaaagaggac
     2221 agcaaaaaac agcacttcat gttttgctgc tggctgattt tattgccgca gaatcaacaa
     2281 gataaacgcc gtttaatcaa gaggctgttt ttcttgtgga ctgattcaag caataaaaga
     2341 agttagaata accctagtat ttgtatttat ggatagttaa atttcaaggc taagcacgca
     2401 aaaagaactg tgacaaaagt cttgtcacag ttctttttta cgaataaatg gcgttcaaga
     2461 gacaggctat cgacaataag gcataatttt gtgaaatatg ggaagctatt tttcaaaatc
     2521 gcttcttatt gctcctgtgg gttactcgtc gcaatgtttg tgagagtttt tatcatcgct
     2581 caaggctgaa cgtctctttc acaacctctg atacacggtg ttccaggagt agattcttcg
     2641 aaaaataagc cgaaatttca caaaaattta agaagcaatt ttcgtaaatg ccttcttatt
     2701 tcttgaagct gaccacttct gacacaacta ccattttgcg ttcagtacat aatcaccctt
     2761 tgacagaacc tacgagtgct cctttgacaa aatatttctg aacaaaagga taaacaatca
     2821 gcataggcac tgttgcaata acgataacag ccattttaac tgtttgtgct ggtggaatca
     2881 catcaacggc actactttct gcttgcatcc cacttgatac aatgacgatt tggcgtaaaa
     2941 gaatctgtat cggccattta gatgaatcgt tgatatacaa aatagcagtc atataagtgt
     3001 tccagtaagt aacagcataa aaaagcgaga tagtagcaat agacggtaaa gctaatggca
     3061 gcataatttt gaaaaataca ccaaaatctg tgcaaccatc catttttgct gattcttcca
     3121 aactatccgg caaggcttgg aaaaagtttc tcataatgat catattcatt gcattaacag
     3181 ccacaggcag gatcatggcc cagtaagaat tgaccagccc tagagaacga acaactaaga
     3241 acgttggaat catccccccg ctgaacaaca tagaaaatac aacaagaaag ttgacgaaac
     3301 ctcgaccaaa caaatacttt ctggaaagac cgtaggccat cagtgaagtt gcacacatac
     3361 tgacgatcgt tccgacacct gtaacaccaa tcgaaacagc aagtgctcta aaaatggtcg
     3421 gcgtagaaag aatgtagcga tacgcgtcta aagaaaaggt agtaggaaaa aggataaact
     3481 ttttcgcgac gacttcttgt gtagaagcaa aagatgtagc gatgatattt aaaaaaggaa
     3541 cgatacagat caaagctagc aaaattagaa acgtattgtt gaaaagtgta aaacttcttt
     3601 ctcctataga gactttcttt ttcatataga taggct
//
LOCUS       DACDKL010000205         3562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDKL010000205 DACDKL010000000
VERSION     DACDKL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.207"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            10..255
                     /locus_tag="IV329_002468"
     CDS             10..255
                     /locus_tag="IV329_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP8941624.1"
                     /translation="MIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(2190..2855)
                     /locus_tag="IV329_002469"
     CDS             complement(2190..2855)
                     /locus_tag="IV329_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941625.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2981..>3562
                     /locus_tag="IV329_002470"
     CDS             2981..>3562
                     /locus_tag="IV329_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8941626.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDV"
BASE COUNT         1172 a          549 c          647 g         1194 t
ORIGIN      
        1 agtgatgatt tgattcatgt cttttccaat gagtggtttg tttctgagaa agaattacat
       61 gcttctaatc tgcaatatct accaggtgaa gatccaattc ctaatatgaa agcaattatc
      121 aacagtaagg attacgaagg atataaagcc aaacatccag aagcgaagcc atttaaatac
      181 ccacaagaaa tgaagcgagc atggcgtaaa atgttagatg atgagttgat tccacttgag
      241 aatgaattga ggtaaacgag aagagttaag aaaattgtac gattcaaaga tggtagtcaa
      301 gtggaagtgt agaaaatagg gatagatgtc ttttatttgt tcacatttta taacgaagta
      361 tttaagacag ctattgaata atgtttcctc cacctttttc attcatttac cccaccccgt
      421 tgttatatga ccgtgttttg agaggaacca ataaaaagca taggctaact aggggttcct
      481 ttttcttttg tttttttgga atccgcttaa gtgaaatgta tctgaaatga aacggagata
      541 aagtgatttt ttgctataat gacaaggttt tagctgtttc agataccatg taacccaaaa
      601 tagtttattg agaaaatagg agagtatttg atactaacta gtaggaaaaa atgagataaa
      661 aattaaatgg aatcatttca ggtaatctca aataaaaaaa tatacagtcc tgtggcactt
      721 tacctatggg actgtatatt ttttactctc agcgggcttg ttttgactcc gtgtctgact
      781 ccgtcgtatt gattttaaat gatattcgat gaccatttaa caggtcattt ttgaaatttt
      841 tgtctctata tgactcagtt tgataattat tgatagatca atttgggtct atatcgaaca
      901 agggaataat ccaataaaaa atgatttaga atatcaaaat aacaaaaaag aggactacct
      961 gtattcgtcc tcttttttga ccagattttg accagtctaa tttacattct aatatattca
     1021 tatgagctaa ttttttgatt aaatggtaag tttttccttg tttgttttag tttaaactag
     1081 tgtgtaattt aaaaatctat atggaagggt ctgtggggta atacttaaat aaaggctagg
     1141 aacctgataa catcaaggtt cctagccttt ttataataaa atgaagcgaa aataatttct
     1201 tttaaagagc cgagattatc agctatataa gagtgaattt gatgcaaaaa acagagccga
     1261 aacagtcttg gtgttcgtag ctctgcttat atgtttcgaa tttaagacaa agagttggca
     1321 aaagatgttg ataaggaaga taagataact gtgttatata aactaggtaa ctaactgtaa
     1381 caagctaatt ttaagagatt cttattagaa gacgtttagg aatataacta agaaaatgat
     1441 tatacaggta attgtcattt caataatgtt ttctaatttt cgcgtctttg ttttaagaaa
     1501 aaggccagaa tcagttggaa aaaagttcgt acgatagtga agaggggcaa aatttcctac
     1561 taatattccc actaacaaga gataaaacgg ccaggttggt gtgattcctc tataaaaaaa
     1621 tgcccatgct accgaaatcc catagattaa caactgaaca atttttacaa tgaaaaaatt
     1681 gttctgccat tactcaaagt ctcttcctcc ttgtatcgtt tatttgtttc attttatcat
     1741 tatgataggc gcttgaacaa tccattttgt attttattga gtactcttgg ataaactacc
     1801 agcgattatc atatttattt atgttactgt aagccatcat ataaaagtaa atgagcagat
     1861 catagaaata taaaaaagaa ctaaaattga tctaaattat gtgcagcttg actacgtacc
     1921 aatctatatt acttactaga ttggtcgtta atctattcgg atcgaatgag caaaatattt
     1981 ttgtgaatgt ccatccttga ttatgattcc aaaaagaatc attgggaggc tgtggcgtaa
     2041 atattatact ataagccaga aatacctatc attctgaact tttttattta ggaactttca
     2101 tctcaaggct ttttcaatta atttgtaccc ctgttttttg gataagatca tgtgcattga
     2161 ttttttctat ctctacgttc atctcctact taatgcctat ctggctcctg agggcttctt
     2221 tttaaggcgt cgtcatagtc ttggctgagg atttcttggt aatctgaggg aatggcttcc
     2281 caagtgagca ggcgaacgtt catgacgtgt tttcctttgt gtttgattac agcatggttt
     2341 ttctcttttt ctacctctcc agcgtgaaaa gacagccatg taccatggcc aaagatatca
     2401 ttacataccc aacgttcgat ggaattgtcc acgtatccta cagggagtgt ggtatagccg
     2461 atttgtggtt tcaaaaaagg attgaatcga tcgatcgttt catttttgta ttgataattg
     2521 ataagaaaaa gatagaccaa acaaaagacg agcagaaaga aagatagtgt ctttttgagt
     2581 gagaagggtt gttttgatga ataagatttc ttgttaacaa gaggttcatc tggcaatgtt
     2641 ggttctattg attcggtagc tgattcaata gaaggaaaat caatcggcag acctaaagta
     2701 tgatgtaaaa gtgacagcat ttcaaggtct ggcattgtac gagaattttc ccaactagaa
     2761 atcgttttat cggaaacacc aattttttcg cctaattctt tttgcgtaag cttacaattt
     2821 ctgcggtaag ttcgtatttt ttctccggtg ttcatcttct tctcctttcg attctacagt
     2881 atatagattt attctacatg acctatacac ttgatagata cgtttttttg atgaaaaacg
     2941 gtatgatttt atcaaagaat catttaagta agggagagtg gtgataatca aaaaaaagtt
     3001 cacagtttta ctcacaatca tagtgaccgg tatcatgagt ggttttgcct tggttagtat
     3061 aaataaacag gtactctatc ggacaaatga atttttccat caaacagata cttatactcc
     3121 gattcgattg ccacaacatt ttattgggca agaagaatat gagcagctaa tcatggtatt
     3181 tgaaaatgtc tcatcagaaa ctgagctttc ttatatgaaa aaaacagtaa atgaatccaa
     3241 tgaagtagat acatcttgga acttcaagat acagccaaag cttgaaatga cattgtatta
     3301 tcaaggggat gtcaaatctt ctcacgagaa ggtaccctat tctaatacct cctttcgcgt
     3361 aacgtccatt gaagaatcga tcaaaataaa agattttgaa ggtgaatttt tcttgatgac
     3421 gagagaaaaa gaagcctatg aacggttcat ttcactattc actgatcgat tcaatcaagc
     3481 atttcacact gattatgcca ctaatatttt tgaagacttt gaaaatagct attcgtattc
     3541 ggttatttca aatacagacg ta
//
LOCUS       DACDKL010000206         3541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDKL010000206 DACDKL010000000
VERSION     DACDKL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.208"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IV329_002471"
     CDS             <1..382
                     /locus_tag="IV329_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941627.1"
                     /translation="DSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEFGDLS
                     GEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAISIKD
                     LKELLNSKRNELKQIKETNSVELE"
     gene            396..1085
                     /locus_tag="IV329_002472"
     CDS             396..1085
                     /locus_tag="IV329_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941628.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1210..>3541
                     /locus_tag="IV329_002473"
     CDS             1210..>3541
                     /locus_tag="IV329_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8941629.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQE"
BASE COUNT         1199 a          467 c          747 g         1128 t
ORIGIN      
        1 agattctgtt aattcaaacg ctcaaataaa agatcaaaga gtagctgaac ataataataa
       61 aaaaatgagt ttgaccaaaa atatctattc tatgtcagat gaagaattgt ataaagaatt
      121 tggtgattta tccggggaac agttagaaga aagtattcca ggaattagtg actcagataa
      181 aattgttgtc ttgtctgctg gtgggcaatt aatgggagat ggcaaatccg aattagttag
      241 ttataaacaa aatcacagac agtttattga tgttaatact gattccaacg ctatttcgat
      301 taaggatcta aaagagcttt taaatagtaa aagaaatgaa ttaaagcaga taaaagaaac
      361 taattcagtg gaattagagt aaaggaagat tcattatgaa aaagattttt ggtgtttctg
      421 ttgggctact tatgatgtta acatttggaa atgcagtata tgctgctgag aaagaagtta
      481 tttcagatcc agagcagtac gcctcacgat actttgctga ttttgaagaa gaagataaag
      541 tacttgcatt gccagaaggt ggttttttaa aaggggaagc taaaattgta tctatggata
      601 atccagatat aattcaaggt tcatataatt cagaaactga tcctgcttca gtttcagtga
      661 cagaagcaaa aaaaattgtt gctgaaaaat tcaaaaatga tattgaaact actccaatta
      721 tgcctcgagg agcatttcca cccgatgttg gtactagcgg acaccttctt gtactagcag
      781 gaggaggaag ctataaatca cagccattta gtggtgcggg atggcgtttt ggaggtatgg
      841 gtgtaaaacc agctgatggt acaggcaact ttttacgttg gaaatcctac attgacgatg
      901 gaagagtagg aaatgtaaat caagccaacg caactaaatc tggttctatt caaggaatgg
      961 cattgtatgc tcgtgaagga ttcaaatact ttgcaccaaa cgggcctggt tggacaatga
     1021 atataggtat ttattacact tataatcctg ttgcaggtac gtattatgtt gttcaaaata
     1081 tttaataaaa cagcacaaag agatttcttt gtgctgtttt attattttta gttgttgaca
     1141 aacactaaaa tataaaatat aatcaacaca taaacgaacg tatgttcggc aagaaagcag
     1201 gggattttta tggatgagat ggttttaagt actcagaaat ggctaaacaa gaaatacagt
     1261 aatgttacag ggtttgataa ggttccagaa aatgggcgaa cagggtggcc tacaatctat
     1321 ggacttattg aaggactaca agtagaactt ggtattacta atctagtagc taattttgga
     1381 cctaccactg aaaaaatgta cgataatcag gttactccta aatggggcaa aaatctacct
     1441 aaaaatattg tatttcttat tcaaggtgcc ttttggtgta aggggattaa tccaggtggc
     1501 ttcgatgggg tatatactcc atatttagat actgctgtaa aagagttgca gactgatgca
     1561 gggtttaatg gaagcgctgt aactggagtt ttggatgcga aatgggccaa agcgttattt
     1621 gatatgagtg cttttgttct tgttcccggc ggagatagta aaattagaaa aatgcagcaa
     1681 tggttaaatg taaattattt agaatacacg ggaataatgc cttgtgatgg tatttatcaa
     1741 agaaattcaa atcaagcatt aatttttgca ttacaagctg aactaggata ttctcccagt
     1801 gaagcaacag ggtcttatgg taatgggaca actagtaaaa cttatcctgt gtctgaaggt
     1861 aactcgggaa actatgtaag aataattcaa tatggattat atgttaatgg ttattttgaa
     1921 gacggtacat tcgatggtat tttcactaaa tatatgggtt tagaagtact ggcattccga
     1981 aaatttatgg tattgcctga atatactgaa atagcagatg ttgttgtaat aaaaggacta
     2041 ttgtctagcg ctggagatat tagaagatct gcagatggcg cagatacttc tacgcaatta
     2101 actagatctc aaatccaaac attggttgat aatgatataa aaatagttgg tagatatctt
     2161 actggtactg ttggaattgg aaaagatgaa cgaaataagt atctaactac agaggaattg
     2221 aataatattt tttctaaaaa tttatctgtg tttcctatct atcaagatgg aggtgcagca
     2281 ttagcttatt ttacttatga tcgtggctta tcagatggta agaaggctat agatgcagca
     2341 aaaaatctaa atataccatt aacaacagtt atttattttg ctgttgatct agacatgtta
     2401 ggggaagaaa ttcttgcata tgctggagaa tattttaagg gggtatctgc tgtaatgtca
     2461 tatagcgggt atcaaacggg tgtttacgga acaagaaatg tttgttctca aataatcaat
     2521 aacgggtatg cttcgtttgc atttgtttca gatatgtcga ctggttatag tgggaattta
     2581 ggttttccta tgcctagaca atggtctttt gatcaatttg ttgaatttac cataggaagt
     2641 ggcaacggag ctgttggtat cgatttaatc gctacatcgg gtagagatag cggatttaat
     2701 gagttatcaa atgatactaa taatgataat tacattgcca aatacaatca gaaggttata
     2761 aatcagatgg tacgtgcgta tcaatatcta tcacaagctg gtcttgataa tccttggaac
     2821 cctcatttga cattttatag atatgtaaat tattctggtt tgtcatggga catcatttct
     2881 tctcctgtaa cagagcatga tagaaaaatt tatgatgaat ataggaataa attaactaat
     2941 agcgagggcc tgtataatta ttttatagat cctaatactg gttcaataat aggtttgcct
     3001 cacttaattg tgacgttaca atcacagatg tttctagccg acacaatcaa tgatagtgtt
     3061 tctgactttg caggttggtt aggagattta atgacttgct ggggtgaagt taagaaatta
     3121 ggaatacaaa tggagcaagg tgtatttgca ttagtaggta cggctgcagg tggaacattt
     3181 agtctagaag atttatacca agatgttgac gcggtaaatg tttatacctt agcaaaaaga
     3241 aattctgatt caacagcttt atcggcttta aaaacgtact attgtgataa gggtgctatt
     3301 gatagattta atcaatttgt taagaacaga tttggttctt cagataaaat cgcttcagta
     3361 acaaatagtt tattagaaaa tgacgaaaca gttgatatct taggtgtagg gataatttca
     3421 tttttcattg ataaaatagt taataattca cttgggacag attttactta tcttgatttt
     3481 attggagatg aggggaaagt tgcaaatgga ttttctgaaa aaattattca atttcaagaa
     3541 a
//
LOCUS       DACDKL010000207         3532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDKL010000207 DACDKL010000000
VERSION     DACDKL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.209"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..1925
                     /locus_tag="IV329_002474"
     CDS             12..1925
                     /locus_tag="IV329_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8941630.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            1930..2931
                     /locus_tag="IV329_002475"
     CDS             1930..2931
                     /locus_tag="IV329_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8941631.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3133..3339)
                     /locus_tag="IV329_002476"
     CDS             complement(3133..3339)
                     /locus_tag="IV329_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8941632.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1319 a          552 c          739 g          922 t
ORIGIN      
        1 cataaaaagg agtgaatagc ttgagaacaa aacaagtatt aaagggagcg ttggtcatct
       61 tatttgctgt aagtacaagc gtcccgcaat ggacagtata tggagagagc atttcttccg
      121 aatcggaatc tcgggaggta gaaaccacag aaactactga tgctggaaat gaaacgaaat
      181 cgagtacttc acaaacagaa acaaatactg aaacaacggt tgagacatct gttactagca
      241 cagatcaaga aacgaccgct accacagata accaacaaac agaagaagca acagctacaa
      301 aaatgtcaaa cgacgaatcc tcttctgcta aagtaagtga caaagagaat gggacagaaa
      361 atgatgatat cggtaaaggt gaaaatttca atggtaaaga atatttgaat tacttcggta
      421 aacagatgac agaaatactt aatggaccaa cagaagacat caagtcaaaa ttaatagagt
      481 tattagtcac tgagcttgct gttaatgatt atggtgtaga taaagagatt atcaaacatc
      541 tgattgctac ttatctattg tacgatatcg cttttgcaaa aaacagtgtg ttgatgtcga
      601 tgcttggagg tacagatgat gggacaacgc ctacagaaaa cacaggcttc tatccaaata
      661 caaaacaact tcatcaaact gtactaaaca aacaagatgg caaaatgatg gatgtatatg
      721 catattatgt cgatcaaggt tcagataaaa cagtcatgat ccatggcgga tttcgaggga
      781 attggaataa cgggatcgtt acagaagaat acaatgattt ttataaggcc gggtataatt
      841 tattatttgt tgattccaga gcgactggta atagcggagg cgattatgtg acctatggac
      901 aatatgagtc agatgacgtc ctttactgga taaatcaaga agttcgagag cggccatctc
      961 aaaagatttt actttatggt ggttcgatgg gagcagctac gatgatgtct gtattagcga
     1021 aagatattcc tgtcaatgtc aaaggaatta ttgaaaattg cggatttgca tcaattgatg
     1081 aacaactacg ttttacgtac agccagacag tagcccccgc actaccggat gccataaaaa
     1141 atcagttaga catcattggg gatcaagagc atgaagattt atttatgggc cttttgaaac
     1201 aatactattt tgatcaggag atgcatttgg atacaaaagc tgctctacct acgattggaa
     1261 tgtcagattc attgcctaaa ttgattatcc atggaacagc agatgatgta gtacctgtgt
     1321 ctaacgcgca aaaattatat gagttatcag gagggtataa agatctttta ttagtagaag
     1381 gagcagggca tggaaaggca caggaagttg accatgctgc ctacacgaaa catgtaacgg
     1441 actttttgaa agtagtgttc catgatcaga taaacgtaaa gtatgtggat gaaaacaata
     1501 aaagtctact caaagaccag gacgaaatcc agctttacgg agcttatgga gaaaattata
     1561 tgacagagca aaagacattc gaaggatacg aattagcaaa tgttgaagga ccgactgaag
     1621 ggatttttaa tgagacgata ccaactatta ttttctatta caaaaaaatc ccagtagctc
     1681 caccaaaaaa gcaagatccg acagagaatg cagacaataa gggaaagaat gaacctcgag
     1741 aggatcaatc aagtgatgat acgcaaccga ttcagatgac agtgaaagta caaaaaaaga
     1801 cagatgcaaa aaaaagaatg ttgccgaaaa ctggagaaaa gaaacattcg cttctcattt
     1861 attcaggtgg atttttatct gttagcacga ttatcacatg gatttggcga agaaagagag
     1921 ggtaaatgaa tgaaatggtt caagcgaata gtgattgcag taccagttat catcgtagtt
     1981 attttagtca ctgtattttt gattaatcaa atcacaccaa agccgattag cttgcttgtt
     2041 agaaaacaat ttgacacaag tgaaaaagaa caagtatatg cccgaccaga tgatttccag
     2101 caaaaaatcg aacaaatcga tataaaaaaa gaaatatcct atccttcaaa gtacaaaaat
     2161 aataatatgg atatctacac accgaaagtg gaaaagaaaa aattaccgat tcttttctgg
     2221 atgcatggag gcgcatatgt aggaggggat aaaaatgatt gtcgtgatta tttggaatat
     2281 ctatgttcag acacgcagca aatcatcgta aatatagatt atgaacgatc accggaagca
     2341 aaacatccta caccagttat ccaattgaac gaagccattc aagcagctaa aaaagagata
     2401 aaagatcaag cagattggtc gaatatttcg attggtggaa attcggcagg tgcacaaatt
     2461 agtggagaat atttgttagc acttcaaaat gaagaaatta aaaaagctga tcatcttgat
     2521 ccgactttgg agaacaagca gattacgaag tttatttctt tgtctggtct gttggatcct
     2581 tctgcactta ctcaagtaag tgataaaatc tctagctttc tttatgaaaa atgtggatgg
     2641 gcttactttg ataataagga ctttgaacaa tcagagaagg tcaaaagttt agccttggtc
     2701 agacatgcgg atcgttggac gcaaaacaca ttcttaacgg atggaaatac aaatacgttt
     2761 acgaaacaaa tggaagaagt aacttctgta tttgagaaag cgggggtgaa agtcacaaaa
     2821 gtagattatg cggagaaaaa tgctgttttg aaccatgaat atcagtttga tttcagcaat
     2881 aaacaagcgc aagaaacata tcaaaagtta gtaatgtttt tcaaggagta aagtcttatg
     2941 atgcaaaaga ggttgtaatc tgtcacttag ctccaagtca caaggagcaa tttcaataaa
     3001 caagcttttc atggtttatg aatattgtaa cttgtgactg aggagttaga gatggcaagg
     3061 ggcacctttt attcgaaatt tgggaatatc acacgattat tgtcacgatc tcttttacgt
     3121 aaattttata agtcagattt gttgttttga tcgtttaaaa aaagcaacaa atccgaagcc
     3181 tgcggaggca agaagggaca aaaagccgag tggtaagcaa aagaatgctg gttccactaa
     3241 tattccgttg ttattgatat aaggctggga acaaagatga atagcaaata aaatcaatgc
     3301 actagcgaaa aaaagcaaag aaatatggat agctttcata agcagcacct ccttttttat
     3361 aattgtatga taaatagatt aagaatctat aaatgtgaca agtttgttag gatgtgaata
     3421 aaatgatcat ctagcgaact tgaaacaaag atggctaatg ataagcaatt aatgattcag
     3481 caatcagata ggtacaaagt ttaccatcat acgtctagac caataataga aa
//
LOCUS       DACDKL010000208         3469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDKL010000208 DACDKL010000000
VERSION     DACDKL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.210"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..641
                     /locus_tag="IV329_002477"
     CDS             <1..641
                     /locus_tag="IV329_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8941633.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(712..2022)
                     /locus_tag="IV329_002478"
     CDS             complement(712..2022)
                     /locus_tag="IV329_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8941634.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2034..3452)
                     /locus_tag="IV329_002479"
     CDS             complement(2034..3452)
                     /locus_tag="IV329_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8941635.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1107 a          716 c          551 g         1095 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata tatcattaaa
      661 aaaaataaag gtcaagctaa aatattattt aagcttgacc ttattataat ttcaacgatt
      721 gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata gataaatcac agcttttgaa
      781 acataagttt tgtactgctt cgattcgccc gaattctttt gcagttattt ggcaatgaga
      841 aaagcagaca tcttctatag gcttttcagg aaatgctact aaatcaaaag ctcgggaagg
      901 aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg atgtgtatgt ctttgacttt
      961 cgtcatttgc tttgattcag gtatttcttc agccactgct tgccaacttt ctggcatttg
     1021 agcaaggtca ttgctatttt ttctattgta ttcattatgc cagtccatta tccaagaaaa
     1081 aggaaattgt acatcaatca tattcagatt ttctaccctg atattttcga tcactccccc
     1141 tctttctttt gaagatttca ttcgaatacc acagtctgac tgaaaaaaat tgatatcgtg
     1201 aatatatacg tcactaactc cagcactaac ttcgctacca atagttacac cgtatcctga
     1261 acggatttta catctagcaa cctcgattct tgatgatttt cgattgactc ttgcaccatt
     1321 gccatctctg ccagacttaa tagcaataca atcatcacca caggccaaat cacattcata
     1381 gacacgcaca tttgttgaag aatcaatatc aatcccatct gtacttggtc catcattgtt
     1441 gcggatgatt actttttcta ctaatacatc gttagaataa gtgatttgca agttccaaaa
     1501 acctgatttc tgaaaaatca aatcacgaac aacaacatgt tctgcatgat acagtaaaca
     1561 agcgcgtgga cgtttaatag cataatccgc aatccaacga agtccctttt gatcgtattc
     1621 agctcttgtc ccgctttttt gatcttttcc ccaatacagc tcccaccaat ggtctccttg
     1681 accgtcgatt attccttttc cttctataag aatatctttc gccgagagaa cattcaaaat
     1741 cgctgctggc catttcattt ctacgccggc tactcgtgta tcgatttctg gaaatgcttc
     1801 gatatctgtt ctccccttga ggactgcacc ttcttcaaag ataagatgac tcttatcttt
     1861 taaaaaaagg gctccgacta aataaatgcc ttttggaata aatacaggta agtgttcttt
     1921 cgcaccttga tcgattgctt tttgcagtat tattgtatca tcttgttgag tgtcgtttgt
     1981 tttaaagttc aaagcattga taatcccttt ttttgtccgc aatacgtttc tccctatctt
     2041 tcattgattt ttttcaattc ttcaccaata aatacagttt ttgaacgatt cagtgggtaa
     2101 cctactagca aaatgattgc ggatacaaat aagcagaatg ctggtaacaa gtttgccatc
     2161 gtataaatcc tttcttgtac taaaactgat tggatggctc cacctgttgt agacgattgg
     2221 taaccaatga gtgccagcat aaatccgcct accgctcctg cacatgcttg tcctactttt
     2281 ctcgcaaaag aatttactcc atatactgtt ccatcttctc ttaagcctgt cttatattga
     2341 tgataatcaa tcacgtcagt aatgaatgcc cagaccatca aattgaataa accagatcct
     2401 aaagtagcga taaataaaag aatcagatat acccatgcat ttgttatatg aaggaaatac
     2461 atcagcaagt acatacatga agaagcaagt aacgcaaata cgcttgcttc ctttttacca
     2521 aataattttg tcagctttgt agcaaatggt gctaaaacta ctactgttcc gtaagtaaat
     2581 aaaagagcaa cagacattgc ttgtttatta tggaagtaat cattgaataa ataagtcata
     2641 ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt caacaatgac taaaataatc
     2701 agtgctcgat tagctaacaa accttttacc aactgagaag cagatacttt ttcgtttttg
     2761 acaactctta ctctttcagt agtcaattta caagttaaca tatatgcgac aaatgctatt
     2821 gcagcacatc cgattgaaat ccaaaagaaa tgattccctg aaacgacttg ttgtccactc
     2881 ttgttatggg tatacattaa aatcggaatg aagaatcctg tagttgatcc gccaatcgca
     2941 gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat taggatttga gctaattgct
     3001 gctgccattg ctccatacgg gatattgatt gttgagagaa aaatcccata aacaatgtat
     3061 gaaacaaaaa tataagcaat cttcaccgtc attggaagat gttggacgaa tggcaaaaac
     3121 aaaatgacag tcactaagca aaatggatac ttcatttttc tgatccaagg aataaatcgg
     3181 ccagtttctg ttaactctga gacatcacat aatcttccaa ccatcacatc agcaaacgca
     3241 tccaaaattc tcgaaatcaa gaataacgta cctacgattg cccctgaaac gccaagaaca
     3301 ttcgtataat aaatcatcaa gaaactattg actaaaccta aaataaaaca gttacctaga
     3361 tcaccgaaca tgtagcctat cttatcggaa atcccaaatt cctttttttc tttttttact
     3421 gtatttgctg taacagcatt ttgtgaaagc atttgaattc ctcctacct
//
LOCUS       DACDKL010000209         3356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDKL010000209 DACDKL010000000
VERSION     DACDKL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.212"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..437)
                     /gene="mscL"
                     /locus_tag="IV329_002480"
     CDS             complement(<1..437)
                     /gene="mscL"
                     /locus_tag="IV329_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8941636.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQT"
     gene            689..1957
                     /locus_tag="IV329_002481"
     CDS             689..1957
                     /locus_tag="IV329_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8941637.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            1950..3236
                     /locus_tag="IV329_002482"
     CDS             1950..3236
                     /locus_tag="IV329_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8941638.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
BASE COUNT         1128 a          607 c          677 g          944 t
ORIGIN      
        1 gtttgctcta ctggatgtgt attgtcttct gccacttgtt tggcaagaag atcgcgtatg
       61 tcactaagat aatcttctgc agtaggagtt tcttccactt ccacttgctc ttctttggag
      121 cgtaaattct tcgcacggtt tgccgctttg acaactaaga ataagacaaa accagtgatc
      181 agaaatgtaa tgatagcact aataacgtta ccaaacgcaa atgtgacgcc ttttgtcggc
      241 gaccagtcaa gaattgaaag tgctccttct aaatcagaag tacctgtgat cattgcgaca
      301 aaccaaccta ttagtggtgt gatcaagcct tgcaccactt ggtttacgat gcttgtaaac
      361 gcactaccaa taacaacgcc gacagcaaga tccaagacat ctccacgcat gatgaactct
      421 ttgaattctt tgatcatgaa tgaccctccc ttcttttatt tgtctttagt atatcgaaaa
      481 ataaaaaata aaaaaacaat atacgctttt agccgtctgt ttcttttgat aaaaaacatc
      541 tatgctatac tataaagtgt aacatcattt agaaaaaacg gcgaattgcc tgaacttata
      601 aatttcaagc agaggaataa gtaaaaaagg caggttgacc cattaaaaga gtaagcgggc
      661 aatcaactgt gaaaaggagc atataaatat ggtgtatcaa ttggcagaag gcgtgaatct
      721 tcatgtctta ccaacaaaac aatacaaaac gatccggatt tttatccgtt ttactgcgcg
      781 tctgcaacaa gaagtcatca cgaaacgttc acttctcagc agcatgttgg aaacaaatag
      841 tttgaattat tcggatcaga caaagttaag tgcgcaactt gcagagcttt atggcgcaag
      901 ctttggatta tcagttagaa aaaaaggaaa tttacattgg ctaaacgcag ggatcagttt
      961 tgtgaatggt gaatatgtca atgatccaaa ccttttttct caagcggtag attttttaaa
     1021 agaggtttta ttttatccaa atattaaaaa tcatcagttt gatcagttga cgtttgactt
     1081 ggaaaagaac aatctgcgtc tatatttgga aagtttgaag gaagataaac aaacttttgc
     1141 ttcctatgcc ttgcaagaac tttattttga aaacagcccg gaacaaaaga tcccagggtt
     1201 gggcgtagtc gaagatttgg acaagatcac tgctagttcg ctagcgtcct actatcaaga
     1261 aatgatggca aatgatcaaa tcgacatttt tgttgtcggc gatgttgatc cggacaaagc
     1321 agctgaagca gttgggcaat taccatttga gtcgcgggaa acagtttatc cagatctgtt
     1381 ttatacccaa cctcaagtga atatcgtgaa ggaacgacaa gtacgtgagc caatcgttca
     1441 agcaaagctg aacctagctt atcagacaaa tgtgtattac gatgaaccag aacgttttgc
     1501 gctaatggtc tttaacggtt tatttggcgg ttttcctcac tctaaacttt tcatgaatgt
     1561 cagagagaaa gaaagtctag cttattatgc ctctagtagc gtggatacgt tccgaggatt
     1621 catgagtgtc caaaccggta tcgatgaaaa gaaccgaaat caagtacttc atctgattca
     1681 cgagcaatta gaaagtttgc gaaatggtga aataacagag ttggaactgt ctcagacaaa
     1741 agcaatgctg agaaaccaat atctgctttc tctagactct ccgcaagcgg caatcgaagc
     1801 ttcttttttg gacagctggc tgccggaaac aaaattatct gatgaagaat ggttgaaacg
     1861 tatggagagc gtaacgatca aagagataca acaagtagct gagcaaatcg aattgcaggc
     1921 aatcttcttt ttagcaggag gaaatgcaaa tgaataaaac agaatatgag caaatcaatg
     1981 aaacacttta tcatgaagta ctgccaaacg gtttgaccgt gtatttgttg cctaaaaatg
     2041 actatcacaa gacatatggt ttgttcagta cgaattatgg ttcgatcgat aacgaattta
     2101 ttccctatgg tgaaaaagaa aaggtgaaag ttccagatgg aattgcccat tttttagaac
     2161 ataagttgtt tgaaaaagaa gatggtgatg tctttcaatt atttgggaaa caaggagctt
     2221 ccgccaatgc tttcacaagt tttacgaaaa ccagctatct gttttctacg acggatcagg
     2281 tagaaaagaa tctgacgact ctgatcgatt ttgtacaagc accttacttc acagaagaaa
     2341 ctgtaaacaa ggaaaaagga atcatcggtc aggaaatcca aatgtatgaa gacgacccta
     2401 actggcggat gttttttggc atactaaata acttgtatcc tacacaccca ttgcatatcg
     2461 atattgcggg cacggttgaa agtatcgaca aaatcacagc tcaagacttg tatacgtgct
     2521 atcggacgtt ttatcagcca agcaatatgg ttttatttgt agttggtaag atggagcctg
     2581 agaaattgat gaaacttatt cgggaaaacc aagaagcaaa aaatttccct ccaaagcaag
     2641 aaattgtacg ttatttccct gaaaacacaa aagaaatcat caaacaaagt gcattggaag
     2701 cagccattac acgagataaa tttgtacttg gtattaaagg attagacaca ttgccgcaag
     2761 aaggaacaga actgttacgc tataaaacag caatcaatct gttgttccaa atgattttag
     2821 gcaatacttc tcgaaactat ctagcgatgt acaatcaagg aattatcgat gacagctttg
     2881 gttttgaatt cagcttggat cgtgaatttc actttgcgga cttcagcgga gatacggacg
     2941 aaccagagaa agcagcagaa aaagtcaaag aaatcattct tggattcgct gatgatccgg
     3001 aagtgtcaga aacaaatctc gatttgctga agaagaaaat gcttggacaa tatttccaat
     3061 cattaaattc gatcgaatat attgccaatc aatttacgca aagtttattt ggcgatcgaa
     3121 ctttatttga tcttcctgaa atcatcgatt cgatccaaat gaaagacgta ctggcagctg
     3181 gggaagcatt tataagtgaa gaggccttca gcgagtttta catgagacca aaataaacca
     3241 gtattttatg aggaaaagtc ttaaatttcg ttttagactt gtagtaggaa cctcctccaa
     3301 aatcagcttt tatggtattc ttagttgtgt tggaaaaatt agtgagaaaa taaaac
//
LOCUS       DACDKL010000210         3353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDKL010000210 DACDKL010000000
VERSION     DACDKL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.213"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(696..1652)
                     /locus_tag="IV329_002483"
     CDS             complement(696..1652)
                     /locus_tag="IV329_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941639.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1761..2006)
                     /locus_tag="IV329_002484"
                     /pseudo
     CDS             complement(1761..2006)
                     /locus_tag="IV329_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2151..2573)
                     /locus_tag="IV329_002485"
     CDS             complement(2151..2573)
                     /locus_tag="IV329_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941640.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2712..3142)
                     /locus_tag="IV329_002486"
                     /pseudo
     CDS             complement(2712..3142)
                     /locus_tag="IV329_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1026 a          722 c          544 g         1061 t
ORIGIN      
        1 aaagattgaa ctatctacat tcatactcta taatctgttt atttgcagac tgataatagc
       61 aaaagaaaac ttataatcaa ataatatcaa catttgatca cataatttga gattgaatgc
      121 tgatttacct atgttttacg taatggtctt tagttatttt tgctcataaa atgcataaaa
      181 cacgcgtttt tatgtgtgct tgaaatttct tggctctata atttctaaga ctgataaaaa
      241 tattctatcg gttctttttg caaataaaaa aacatataga agctccatta gattgaatca
      301 ccacaacaca atcaaaggag cattccgtat gcttgatttt cattctataa aactactttt
      361 aggaatcaaa gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga agtaccattg
      421 tttcatcgat catgagaaaa gcattcacct ctgtctgcag atgttcttat atcgctctgt
      481 cagtcctatt aataaagtac tgttttgttt tcaaaaaaac tattacgagt actttttaag
      541 agaatgcctt gcaaatattc atcacacacc gttatataag tcaaatagtt tctttcagtc
      601 gtcaagaaaa gattctcctg tccttggcat tatcagttaa gtagcaactt aattctcgga
      661 cttgttctac tctagcaaaa tttattttca tcaacttacg tccaaatgtt tttttgaata
      721 attacttcat cgcgggtctg gaagacttgc ctcattcgat aagtcactag cttttggcgt
      781 ccttcaggat ttctagtata caccaactga catttgggca gcgacgattg atttaattcg
      841 tttagaaaaa ggttagcgat ttgcttattt cggccaaagc gttctggtac cgtcagaact
      901 atctttttcc cttcgattaa ataccgcgga ttctccagcg gtgctaaaag ctccctcata
      961 gcttcagtaa aaagctgtcc ttctcgatag ccagcattct caagatagca tctaaattcc
     1021 tgctgatcag ccgctacgat tacttttcta tcactaactt caccaaggcg ctgcaacgtc
     1081 gtttgaacgc atatggccaa gctttttttg cgggcacatt ccttttccca tagcttttgt
     1141 cgcctacgaa tttggaaata ttccagcaag ttacctttaa atatagctga ccagaagctt
     1201 agaggctcta tattttttac tactatcggc aatgaagttt cttgtttttg ctttacctga
     1261 tcgactttcc taacgacgat tggcatagat aggtgccagc catcagctaa agcagtactc
     1321 cattgttctt tcaaccgact tcgttcacaa gaaaaataga acatcgactg agtataagtt
     1381 tccatcgtgg tggtcgtata ttccagtggt aaagcaaagc gatcgccacc actctcaatt
     1441 tttggtgaag attcaaaggt cagacctgaa atattgccca aacgccgtag cattcttttc
     1501 aaatcaggct gttcagacag aggaactccc ggtaatccaa tcgctaaatg ccaaatgttc
     1561 gttactttat tttgtgcacc taggcgtaag ccccgccctc gtagttgttg tgtttgcaca
     1621 aaagtggaag cttgattagc caagatcaac agattaattg caggacaatc ccagccttct
     1681 cctagcaaac taacggttcc taccaaaata ttcaacttct tttcggttaa taggcgagta
     1741 actgcttgca tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa ataacccgac
     1801 atataaggat gttcattcat acccgtcgct agttctgcca gctcaacagt tactagcatt
     1861 ttcggtaaca gtaagaactc tccacagatg gcaccgattt gctgagtgtt acctaataga
     1921 cgaacaactt catcaaacaa aggaaccacg ccataagcta aggtaccatc actgcctgct
     1981 aatacctcct tgcaaatacg atccatcaaa atgaccgctg tcagttcttg cttcagctga
     2041 acttcttctt ttaacaaaat ttgacaaact gcttgctttt tctcaggaat cgcctgacta
     2101 ccaacagtgt ctggatactc tggaaacaaa tgcagcgact catcaaacaa tcatccagct
     2161 tttttcaatt gataaaaaat aggcaattcc ggttgatctt caaacatctg ctgataaata
     2221 gtgatcagct cttttttacc taaagatgga actgaagctt ttttaccttg ctgcttagaa
     2281 ctgcctaata gctgccaatg ttcgggtatc aattcatagt tatgatgatt caagtaaatt
     2341 aatgcactag tgaacagctc aaaattttca taaatttcct tcgaatgctc aagcggatgc
     2401 tgaacaaatt ctaaagaaag caaataatcc accgtctctt ggctttccat taacgcttcc
     2461 accagctcat tttgtttctc taaaaaggta aaataatgct gagtagtttc ctgattagcc
     2521 ggaatcaaat atagaaaatc ctgatgagga catagaacct cctcctttac caactcggga
     2581 atgctaattt cttcgtcaat caggccattt taactcaata tattgcttcc actccgtctg
     2641 agagctatca atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg gttgtttttt
     2701 cttcaagtca atcaacaatt caccccagct tttttttaaa tgatgagctt catcaaaaaa
     2761 ctaacagctc cagctgatca ccataagttg gctcctcctt cagtcggttg taaatggtct
     2821 gatacgtttc cactgtaata tgactaggat gttcaatcgc cccagaaaga tagcaactat
     2881 ctgctggatt tactaagaaa tattcctcga ttgttgttaa ccactgtttc ttcaatatta
     2941 gggaaggaac caaaattaag gttttacaat ccaaacgtcg gatcatctca attcctaaaa
     3001 aggtcttgcc agcccctggt ggagccacta gatggactga attgttattt aaataagtag
     3061 caaagttagt caatatacgc ttctgatagc tacgccactg aattttttca tcagatagcc
     3121 acatcccagg tccccctctc acgtattttc tctatcataa tctaaatgaa tacagcaaaa
     3181 aaaagatttc ttcaagaata tgtgttttta tagtaatatt ttacttactc taaactcaaa
     3241 tgaaaaggta aaaaaactgg tagtacaatc ttttaaattc tcattgtttt gtacgttctt
     3301 tactatcaaa aaaacaaaaa aaacgcgttt ctacgcgtga ccaaaatttt tag
//
LOCUS       DACDKL010000211         3352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDKL010000211 DACDKL010000000
VERSION     DACDKL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.214"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..1141
                     /locus_tag="IV329_002487"
     CDS             101..1141
                     /locus_tag="IV329_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8941641.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            1965..2297
                     /locus_tag="IV329_002488"
     CDS             1965..2297
                     /locus_tag="IV329_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8941642.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2916..3257
                     /locus_tag="IV329_002489"
     CDS             2916..3257
                     /locus_tag="IV329_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8941643.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1247 a          457 c          639 g         1009 t
ORIGIN      
        1 acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa
       61 ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt
      121 taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc
      181 tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag
      241 attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta
      301 tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa
      361 aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt
      421 agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat
      481 ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat
      541 gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg
      601 tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga
      661 acttaataat aatatattag acactaatag acacaatata gacactgaaa aagaccgcct
      721 acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac
      781 gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc
      841 tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat
      901 ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac
      961 gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct
     1021 gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag
     1081 tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact ggttagagtg
     1141 aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta aaaatttttt
     1201 attattctaa ttatattacg aatattacaa aataatagct attttaatag aatttataag
     1261 ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg ttaaaagaaa
     1321 taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta
     1381 taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac
     1441 ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg
     1501 ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt tggacaattg
     1561 aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt
     1621 catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc
     1681 cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
     1741 ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta taaaaatgtc
     1801 cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg
     1861 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
     1921 aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa
     1981 ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca
     2041 cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc
     2101 agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt
     2161 taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa
     2221 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
     2281 ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
     2341 ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga
     2401 cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg
     2461 taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt
     2521 aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt
     2581 ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa
     2641 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
     2701 atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact
     2761 agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa
     2821 cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat ataaaacata
     2881 gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac
     2941 gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga
     3001 cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt
     3061 tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg
     3121 aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg
     3181 aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag
     3241 cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt
     3301 cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat tt
//
LOCUS       DACDKL010000212         3336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDKL010000212 DACDKL010000000
VERSION     DACDKL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.215"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IV329_002490"
     CDS             complement(387..1853)
                     /locus_tag="IV329_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8941644.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..>3336)
                     /gene="cadA"
                     /locus_tag="IV329_002491"
     CDS             complement(2144..>3336)
                     /gene="cadA"
                     /locus_tag="IV329_002491"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8941645.1"
                     /translation="KKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVSGAAFSEWI
                     YRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTETVVFDKTG
                     TLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLPTTNIQEFA
                     GEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYLVIADTLKE
                     DAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKVQRLEEILQ
                     RNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPSKIAEAIHL
                     AKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLNAMRSLHVK
                     KEDE"
BASE COUNT         1018 a          701 c          551 g         1066 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttac
//
LOCUS       DACDKL010000213         3320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDKL010000213 DACDKL010000000
VERSION     DACDKL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.216"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..621
                     /locus_tag="IV329_002492"
                     /pseudo
     CDS             1..621
                     /locus_tag="IV329_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1069..1515
                     /locus_tag="IV329_002493"
     CDS             1069..1515
                     /locus_tag="IV329_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941646.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="IV329_002494"
     CDS             1517..1654
                     /locus_tag="IV329_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8941647.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="IV329_002495"
     CDS             1691..1984
                     /locus_tag="IV329_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941648.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="IV329_002496"
     CDS             1981..2205
                     /locus_tag="IV329_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8941649.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="IV329_002497"
     CDS             2202..3221
                     /locus_tag="IV329_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8941650.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1163 a          586 c          690 g          881 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca
//
LOCUS       DACDKL010000214         3314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDKL010000214 DACDKL010000000
VERSION     DACDKL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.217"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..1458
                     /locus_tag="IV329_002498"
     CDS             70..1458
                     /locus_tag="IV329_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8941651.1"
                     /translation="MEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKG
                     FRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEI
                     EAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKP
                     RFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLK
                     YYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELN
                     KLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAH
                     IFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
                     VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVK
                     ERLEQIIGSLTG"
     gene            1465..1842
                     /locus_tag="IV329_002499"
     CDS             1465..1842
                     /locus_tag="IV329_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8941652.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1996..2778
                     /locus_tag="IV329_002500"
     CDS             1996..2778
                     /locus_tag="IV329_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8941653.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2904..>3314)
                     /locus_tag="IV329_002501"
     CDS             complement(2904..>3314)
                     /locus_tag="IV329_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941654.1"
                     /translation="TDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMD
                     IKMLKKVPPLYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTK
                     NQFRQALKHANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         1214 a          479 c          644 g          977 t
ORIGIN      
        1 attactgcta atcaagaaaa aacacctgta aaagctaatt cctactatgt tactaatcta
       61 aaacaattta tggagtttta tgagaacttt tattttgatg gagaggagtg ggataaagac
      121 gtttgggata gacgtaactt acctttgcca gatgataagg ttaacccaac acaatatgaa
      181 tatacaatta actttaaagg gtttcggaat acatatttta aacaacttgt aaaaagatat
      241 tgtaagttga gattgaacat ggatagcttt tcctatgtaa gtgatattgc ccaaagactt
      301 aaagagttct ttaattttct ggacatgaaa tttaaacaag ttcagagagt acaccaatta
      361 acgagagtgg aaattgaagc atatttaagt gaactaaaca tgatgggaat aaaacctagt
      421 acaataactg ggaggatctc tatattggaa ggactattta gtacccttct taggctagaa
      481 tgggatgatg ttccttccaa aatattaatt tattctgagg actatccgaa aataccaaga
      541 gcaaaaccac gctttataga tgaattcgtc ctagagcaat tgaacagtca tcttgataaa
      601 ttacccgaat atatagctac gatgactatg attgttcaag aatgtggaat gaggataagt
      661 gaattgtgca ccttgaaaaa aggctgtcta ttagaggaca aagatggaga tttcttttta
      721 aagtattatc aatggaaaat gaaaaaggag catatagttc caatatctaa agaggtagct
      781 ttacttatta aagttcggga agataaagtt tcagaggaat ttccagatag tgaatacctc
      841 tttccaagaa aagatggatc gccattaaaa caagaaacat ttagaggtga gttaaataaa
      901 ttagcttatg agcaaaatat agtggataaa tcaggtgaga tttatagatt ccatgcccat
      961 gcctttcgcc atacagtagg aacaagaatg attaacaacg ggatgcccca gcatattgtg
     1021 cagaaatttt tggggcatga aagcccagaa atgacaagca gatacgctca tatctttgat
     1081 gaaactctaa aaaatgaatt tactaaattt caggaaaaac tggttaccaa taatggagat
     1141 gtgcttgatc tagatgaaga taatgaagtc gatgatgtag agcttcaatg gttcaagaaa
     1201 aatataaatg cacaagtgct tccaaatggt tattgtagat tgccagtagt agcaggtggt
     1261 tgtccacatg cgaatgcatg cttagattgc actcacttct gtaccagtaa gcaattctta
     1321 ccacagcacg aagaacagtt agagcgtaca gaagagttat tagccatagc taaggataaa
     1381 caatggcaaa gacaagtaga gactaatagc cgtgttaaag agcgtttaga acaaatcatt
     1441 ggaagtttga cggggtaatt atcaatggat aaacaagtta gaaatacaac agaaattgta
     1501 cgtttggcga agcagaaatc aaaaaagaca agggaaaaag tagacaaagc gatttctaaa
     1561 ttttcgattg aaggtaaagt tattaatttt aattcaatag caaaggaagc taatgtttct
     1621 aaatcatggc tttataagga acacgatatt aggcaaagaa tcgaatccct tcgtgagcgt
     1681 caaataacag caaatgtagt ctcaaaaccc aagaaaagtt ctcgttcgga ggaaatcctt
     1741 attaaaacct taaaaagaag agtaatggaa ttagaaaaag aaaataaaaa attacagaac
     1801 caaattcaaa aattatatgg agatctgtat aataaagaat aattattaat ctgtagacaa
     1861 attgtgaaag gatgtactta aacgctaacg gtcagcttta ttgaacagtc atttaggtat
     1921 atgtccaatc tagggtaagt aaattgagta tcaatataaa ctttatatga acataatcag
     1981 ttacgaggtg aaatcatgag caatttgatt aacggaaaaa taccaaatca agcgattcaa
     2041 acattaaaaa tcgtaaaaga tttatttgga agttcaatag ttggagtata tctatttggt
     2101 tcagcagtaa atggtggttt acgcattaac agcgatgtag atgttctagt cgtcgtgaat
     2161 catagtttac ctcaattaac tcgaaaaaaa ctaacagaaa gactaatgac tatatcagga
     2221 aagattggaa atacggattc tgttagacca cttgaagtta cggttataaa taggagtgaa
     2281 gttgtccctt ggcaatatcc tccaaaaaga gaatttatat acggtgagtg gctcaggggt
     2341 gaatttgaga atggacaaat tcaggaacca agctatgatc ctgatttggc tattgtttta
     2401 gcacaagcaa gaaagaatag tatttctcta tttggtcctg attcttcaag tatacttgtc
     2461 tccgtacctt tgacagatat tcgaagagca attaaggatt ctttgccaga actaattgag
     2521 gggataaaag gtgatgagcg taatgtaatt ttaaccctag ctcgaatgtg gcaaacagtg
     2581 actactggtg aaattacctc gaaagatgtc gctgcagaat gggctatacc tcttttacct
     2641 aaagagcatg taactttact ggatatagct agaaaaggct atcggggaga gtgtgatgat
     2701 aagtgggaag gactatattc aaaggtgaaa gcactcgtta agtatatgaa aaattctata
     2761 gaaacttctc tcaattaggc taattttatt gcaataacag gtgcttactt ttaaaactac
     2821 tgatttattg ataaatattg aacaattttt gggaagaata aagcgtcctc ttgtgaaatt
     2881 agagaacgct ttattacttt aatttagtga aacaatttgt aactattgaa aatagaaaga
     2941 aattgttcct tcgatagttt attaatatta gtgacatttg catgcttcaa agcctgtcgg
     3001 aattggtttt tagtgaaaag aacacgatat tcacggttta cccacttata aacaaaagat
     3061 cgatactttt tgtagtcctt ctttgaaatc aatggttgat gtcgttcaag aacaatcaat
     3121 acagagtcta cacttggctt aggatgaaaa tatagtggtg gtactttttt gagcattttt
     3181 atatccatct ccaccattaa tagtaaaccc aaagctcgtt gcagattttg caatcttttc
     3241 gcaaatccct tctcaacgat aagatagcta tatttagcct gactttcaaa ggtaattctt
     3301 ttgacaatat ccgt
//
LOCUS       DACDKL010000215         3295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDKL010000215 DACDKL010000000
VERSION     DACDKL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.218"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV329_002502"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV329_002502"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8941655.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IV329_002503"
     CDS             complement(2261..2512)
                     /locus_tag="IV329_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8941656.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IV329_002504"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IV329_002504"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8941657.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          899 a          726 c          614 g         1056 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aatta
//
LOCUS       DACDKL010000216         3235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDKL010000216 DACDKL010000000
VERSION     DACDKL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.219"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..908)
                     /locus_tag="IV329_002505"
     CDS             complement(<1..908)
                     /locus_tag="IV329_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8941658.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYG"
     gene            complement(1037..1684)
                     /locus_tag="IV329_002506"
     CDS             complement(1037..1684)
                     /locus_tag="IV329_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8941659.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(1768..3195)
                     /gene="uxaC"
                     /locus_tag="IV329_002507"
     CDS             complement(1768..3195)
                     /gene="uxaC"
                     /locus_tag="IV329_002507"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8941660.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
BASE COUNT         1030 a          664 c          432 g         1109 t
ORIGIN      
        1 ttaccgtatt ttttactgaa aattaaactt acaggtgaaa caagtaccaa catcaaaata
       61 ggattgataa agttcattaa aactattccc tgtgcattat tataataatc tggaaataca
      121 taattgttca tactcattgt taaaagctga gcaagcaaca aaaaaatagc tgcagctatt
      181 atagaaagca acgctctatt tttagctaat ccttttaaag atttcaacaa tgaaactttt
      241 aactgcgatt catcactttt aacccgttct acagttaatt tataacatat tacgtaacaa
      301 ataactgcac acactgaaaa tactcctgcc acaattgtaa aagttgatcc accacgtaca
      361 atttgagctc cagaactatc tcttgtataa ataaaaattg gtgttaacac gccaatgaac
      421 atacctgcta aggtagctcc tatactacga aatgttgata acgctgtcct ttcttctgca
      481 acagaagtga tagctgatgc cattgaaccg taaggaatat taatagatgt ataaaaaata
      541 cttccccata gtatatatgt tacatacata tatataactt taatactcat agaagctgat
      601 gccattgatg tttgatacat taaaaaactt gctaaagcga ctggaccact catccgtaaa
      661 atccacggtt taaatttgcc agttttagat ggttttgatc catccacaat acgccccatt
      721 gttatgtctg taactgcatc aataaatctt gctactaaaa ataatgttcc tacggtagct
      781 ccgcttatcc ccaatacttt tgtataaaaa atcattaaaa aagaactagc aaaaatgaaa
      841 gtgaaatcat tcccaaaatc tccaaacata tatcctattt tgtctcttag accaaatttt
      901 ctttccatat aatttgcgct ccttcctatt gttaagcgtt ttcattctgt tgaactgcca
      961 acttacgtac tatactctga agtataaaga gttagtattt attttttaca ttttatagta
     1021 ttatttattt tttctcctaa taattcttta tctcataaaa cttatctata tatttactag
     1081 ctattcgagt tacttcttca aaatcacctt tttcaacagg agccaacaaa ttcccaccta
     1141 caccaactgc aacaacgcct gcttcaaacc actcttctaa gttatctaga cttactccgc
     1201 cagtaggcat tatatttaca tatggtaatg gagcttgata cgtttttaca acagatggac
     1261 caaaagcaga acctggaaat agtttaatga tttctactcc agcggtaagt gcctccttta
     1321 tctcagtaat tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctctg
     1381 ctgtttgtcg atcaaaagat ggacttacga tatattctgc tccagccata attgcaatac
     1441 gagcagtaaa cgcatctaaa actgtaccgg ctccaattat tacatctttt tgatcagcat
     1501 attttttagc aatttctttg attatttctt cagcattcgg aactgtaaaa gttagttcaa
     1561 tccctttaat tccacccgca gtaattgcat ttattgcttt aatggcctca tctttatttt
     1621 ttccacgtac aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt
     1681 tcatatctat ctccttcttt tatggataca tactctagct agttgtacta acttacaata
     1741 aataaattta agcttttatt ttttgtatta ttttctactt tcgatttcaa aaaaattaaa
     1801 ataattatac gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc
     1861 atctggaatt cgacctgctt taacccactc gcttagtaat tgacataata ctcgacggaa
     1921 atactcatgg cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt
     1981 cgacaacaaa ctttgttgcg ccatcgtagt caactgttga cgcattcctt cagctgtatc
     2041 attgaaccac caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg
     2101 cattgctgtt gctagtggaa gccaatcggt tggatttaat gaatataaaa ttgtttttgg
     2161 taagcaccca ctcttttcag cagtgtcgaa caatgctaca acattttgtg tcaaactagc
     2221 ttgatccccc atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga
     2281 attatttctt aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga
     2341 catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt
     2401 ttttcctgct aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata
     2461 aaaataagta ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aataatccac
     2521 tcgttggctc aatgcctgct tcaacccatt aaagtttttg attggtatac ccgctgcttg
     2581 actaagtttt aaaatatagt tcgaaaatcc tgatgatttg atactccaga ccttatctgg
     2641 acggaaagta ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatatcg
     2701 caagtcatct gtaggatcat ccgttgtaca aatggctttc acatccatca tcttcagcaa
     2761 ttcttttggg cgataggaat ccgtttggat tttttcgttg gcacgtcgcc aaatttctgg
     2821 tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga
     2881 ccattcaaaa ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt
     2941 ttcataatca ttacctgcac cactgatcaa ctcttcttct atcccattag ctcgcatcaa
     3001 tcgccattta taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt
     3061 gtattgctta ttttctagca aacatgctgg gtctaaatga caatgataat cgatgattgg
     3121 ttggtgttct gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt
     3181 cttatctaaa aacatattta agcactcctt tccttccctt agtttttaga agaga
//
LOCUS       DACDKL010000217         3231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDKL010000217 DACDKL010000000
VERSION     DACDKL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.220"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..359
                     /locus_tag="IV329_002508"
     CDS             51..359
                     /locus_tag="IV329_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8941661.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            386..685
                     /locus_tag="IV329_002509"
     CDS             386..685
                     /locus_tag="IV329_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8941662.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            859..1410
                     /locus_tag="IV329_002510"
     CDS             859..1410
                     /locus_tag="IV329_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8941663.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1556..1849
                     /locus_tag="IV329_002511"
     CDS             1556..1849
                     /locus_tag="IV329_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941664.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1842..2138
                     /locus_tag="IV329_002512"
     CDS             1842..2138
                     /locus_tag="IV329_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941665.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2209..3213
                     /locus_tag="IV329_002513"
     CDS             2209..3213
                     /locus_tag="IV329_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8941666.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         1212 a          450 c          647 g          922 t
ORIGIN      
        1 cttctttaat agaagattgg gctattttta tggcaaagga gggaaaagaa atgtttcaaa
       61 ataaaggaca acgttatgtc acaaagggag taatggacag tttgccggtg gaattacaag
      121 ctttatgttg gaatctaatc gatcaaaacg ttcaaaagca atttcattta gactatcttc
      181 aaatctttga attttctacg gaaaaaggaa atcaaaagtt ggttcatcgt caagaagaac
      241 cagaagcaag aaaagagtat ctgatttcac ctaagcttcg tttgaaatca gtgtctcaaa
      301 aaatatgggt cattgattcg ggtgagtatc aaacgatgtt attaccagaa gaatactgat
      361 atctaaaaga aaggaacgaa aggacttgtt agaaacggct atcgaagtac tggaaaagtg
      421 tgcacagcta gtcactactc ctgaagagtg gggctatgaa tccgttacga tggaaaaaga
      481 ggaaattgaa atgggtatgc ttcctgaaga tgttactcta ccacgtttag tgatgacaca
      541 tctttatatt tattgcgcac cagaagatgg aaaagactat gtagtttatt tcattacaga
      601 tattaccagc cagcgagaat ttgttcgtgg gttactggta gaaggacgat tggtttggag
      661 tcagattgga ggaacgaatg aataagatcg attatttgag aatgattgcg aaatgtttag
      721 aagatatcct tacagcgatt catcaaattc tgaagaaaaa aggagaaaga atatgaaata
      781 ttttcaacta gtattgacac ttataacggc aatggtgatt gaattgcttg aacaaaaatt
      841 agcataggag aaaaagagat gattgaaaaa gaattagtga ccacacaaac agaagtttgg
      901 cgagatcctc atcataccca tcgctatgtt ttcaaacgac aatgggcgat caaaggaaaa
      961 gaagcggaag ataaattagc ggtagtcatt actattcgtc caacagatac agaaccattt
     1021 acgaatgatt tgagtatgtt attaatcgaa aagaatatcc gacaattagg atttactggt
     1081 tttattgctg tgaatctttt ttcgtataca aaagcgaaaa gtcctcgggt atttcctcgt
     1141 ggaaatgatg aacaatcttt ggaaatatta acaactgttt tcacagaaaa gaaaatcaac
     1201 accattattt ttgcttgtgg ttcgattaca gcgacgagtc aagtggctta tgaacaagcg
     1261 aagaccattt acgatcaatt gagtgcgaaa caaaaaaaga tgaccaaagt attgataaat
     1321 gcagaaggta agttatccca cccactaagt attcatgttc gtaatgaatg gcaattggca
     1381 gatagtcaat tattatttca agaaaaataa cattttattt tcttatgtta cttataagta
     1441 gttttacatg ctttttgact ttaaaaatac atgtgatgat acatgttata tgaattatca
     1501 atgtacccag taatatgcta ttttcattat gtaaaaaata acagggggtg tgtagatgga
     1561 gattgatgaa acttgtttga gtaactctta taaaagtatg aatgactttt ttgaggaaat
     1621 ggaactggat tcagaattgc tctatcaatt ttatcttgct tttcgagggc aacagatttc
     1681 ttttccaatg aaaatgtacg atcgtgagtt agttagaaaa agaatcgaaa acatgataga
     1741 acaggaaaaa actgtagata tccgtcaatt gacagaagtt tatggtttct ctgcaagatg
     1801 gattcaaaaa gtaattaaac aaaaagaaag gagacgagta catggctaag ctgattgtat
     1861 taggtggttt aatttacggt ggatattggt tatataaata tatgcttgcg catccgaata
     1921 tagataaagg aaaagtaatt aagacagttg gggtaatcat agttggactc atagctctaa
     1981 ctaagaaaaa taatactaat agcccaaaaa taatgagaac ttatcgctgt agaggatgtg
     2041 gaagggaaat ttctactagt tttaatccat ctgaaagccc tcaaggaaga gatcgttgtc
     2101 atgctggcta tcgacattct tgggaaagga ttaactaaat gaacaccatt gagattaatt
     2161 gactgataaa ataagtacgc gagggagaaa ttgaaagggg gaaataaaat gaaaaaatgg
     2221 ttgttattac taattggaat ggggatttta ttcactggtt gtacatctga gaatacgagg
     2281 actaaagaga ctgtagaaac tgatacaaca attgaggcta aaaaaagtaa aacgcaatca
     2341 actgaacaaa ctacaacaga aatgaaacaa tctgtggaag caagtacaag tgaaagttca
     2401 atcattgaat cttcttctga agaggttaaa aatactttgt gggatagtga gaaatctact
     2461 caactagcaa tttttgtccc acaatgggga aagacattag gtcaagaata taaaagctac
     2521 aatcctcaga acaatgtaag tctgtatggt acgcctttac caagtgcagt tattgatgga
     2581 aattggaaaa tggcaatcaa tgaagcacca gttaccgtac aatggtcaga agatggaact
     2641 ggtcatgcag atttcaattt agtagcagtt tattctgatg tagaaactgg agaatattta
     2701 gggcagcatg tatatttctt tggctttcaa aatggacaac ctaaagttta tttaacgcaa
     2761 caaaatcaag gcaatgataa aaactacatg tacttcaatg aaactcagaa ccaacagtta
     2821 aaacaaggtt tcattgatat tgtgaatgga caaactcctc aaactccagt agtagaagaa
     2881 tctactcaac aagcaacaac tacaacgaca caatctacaa gtagctcagc aatttcaagt
     2941 tgggaagctg ctaaagaaac tgtactaaat aataaagctt tatggttgta tgattctcct
     3001 gataaaaatg catcaaatac taatttaatt agcacaaatg aatatatgaa agatctaatg
     3061 caagatgaaa atggaagcta ctattcaatt aatattgtaa cggatgataa tggaaataca
     3121 acaatggctg gtggaaaaca attccgtgtt tatacagatg gaagaatttc tcaacgtatt
     3181 ggaatgacta acaattttca aatgattaaa taattgaaaa tattatttat c
//
LOCUS       DACDKL010000218         3169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDKL010000218 DACDKL010000000
VERSION     DACDKL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.221"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..198
                     /locus_tag="IV329_002514"
     CDS             <1..198
                     /locus_tag="IV329_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941667.1"
                     /translation="VGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRDYQLIN
                     ESKYRMLVKFCDISAPAELVS"
     gene            complement(476..2275)
                     /locus_tag="IV329_002515"
     CDS             complement(476..2275)
                     /locus_tag="IV329_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8941668.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2399..2995)
                     /locus_tag="IV329_002516"
     CDS             complement(2399..2995)
                     /locus_tag="IV329_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8941669.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1117 a          588 c          563 g          901 t
ORIGIN      
        1 gtaggaagat cttatatatg caaaccgatc ggagtatctc atgaactaat cggctgtatt
       61 gaacgtacat atatcaacac ggctgtcgtg tatgttgaaa gttatgacca acgagactat
      121 caacttatca atgaaagcaa gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct
      181 gctgaattgg tttcttaaac agtatttttg agaattcgca agaactccct tataatataa
      241 ataaagacag aagggagcga gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa
      301 ttattcttgc agtgatctct gcaattcctg ctattttagc tattgttcgt gcgatgaagg
      361 aatcaagaga aagttaagca cacattatga gcgtcaaaca aaaaacgcat atacctgcgg
      421 tggcaggtta tgcgtttttt gttgtataaa gacttttttc attaacgatt tttgattagc
      481 cgactcccaa aacaaaattt agcagtttat ctgaataagt gatttcttca tctgtttgtt
      541 tcacactttc atttagtagt cccatctcat cggttatgat accatccaca ccataaaaca
      601 tcatgcgact catagcatct gaatcgttga ttgtccatac atatacttct tttccgtctt
      661 ggtgggcaga attaatgaag ttacggttga tcgtactata ttccattgtc agaaaatcag
      721 caggcgtcac agggggccca acaatattga aaggtaagat gtagcccaca taaaagtttg
      781 gttctgcttc ttttaaatct gaaacggttt ggtacgtcaa actttgcaaa atatgatggt
      841 tctctaagat aactttgcgg tatttatgga caaatcgttc gacaaggtca tccgaatctt
      901 ttttagaggt cttgatctca ataagtaatt tttggtttag cttatgtgct ttttccaaat
      961 aagaatcaaa agaacaaaca ggggcttgca ctccgttttc agtaactgta agatttgtca
     1021 attctttcaa tgtaagatcc tgtggtgttc catttacccc tgttaagtga cgaagatttg
     1081 catcatgcat cactacgaaa tgaccatctt ttgtttcctg aacatccatc tctacataat
     1141 caggatgata gtctctgctt gttttttcaa gtgcttctaa agtattttgc gccccatttt
     1201 gggtactcac acctctgtga gagacagtca ctggttttgt ctgaacagct gaagtcagat
     1261 aatccatatt gtaaagacag acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag
     1321 tgtttttcag cgcagagaaa cgcaagttgg gggcttgtgg gataaaccac ttagggatct
     1381 caggcaagaa gccttcgtca tccatgaaat caacaatgat ataaaaaatc cctacggtag
     1441 acatcacgat attgaataat aaaatccctt gtaaaagcgt catagcaaac acagcgctga
     1501 tcaatgcata gtctgggaaa aattgttcaa ccatactttg tgccaaaata acaacgatat
     1561 atcctaaaga agaaaggagt aaaatcgtac cgccaatcac aataaattgg cccgtgatgg
     1621 ccaaaagacg cgatttggtc aatgaccagc tttcccgaat ggctgctcga aaaggacgat
     1681 ctcgtagaat catttccggt aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa
     1741 tataaacgag taaaaaggaa ctgacgatga tccaacgatt agcaaaaata aaatccataa
     1801 tgaaagcggg gatttttatt tttgacagta gatcagaatt aaaacttaat ccgctgatcg
     1861 gtagaatcaa taaaaaataa gcaaggaaaa acaaaaaagt aatgggtctc actttcttca
     1921 gctgaagtat cgtcaagtgt aagagttgct tcaaagagat tggttctttc tttttaataa
     1981 aaaaaacact catcaaaaga aaagtaaatt cgaaataaac taacagtaaa atcgtcatca
     2041 aaactacgat taacgagagt agaaccccag gatgttgaga aaaaattgaa ggaatattgt
     2101 cataagacaa atagtcaatt gcccctcggt gtaaaataaa acgggtcgta ctgctcaata
     2161 agggtaaaca aataaataaa ataaagccat gcatcagcaa gacatcacgg aaataagctt
     2221 gtgttccttt taaaaagtcc caagcattac ctaagctacg tttcaaataa ttcatcctat
     2281 cacctgctct tcacattgta gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt
     2341 gcagaccctt tcccagattt aacattgtca atcaatgact aattttacaa aataaatatt
     2401 atccaagaag accacttaga tattgacctc cataaatcaa ggccatacta tataagaaaa
     2461 tcgacagtgg tttagcaatc aaaatgatta gtgtatatcg tttcactgac atgtttgtca
     2521 atccagccat caaacataac gcatcgtcag gagcaactgg aagaaaaata gctagagcaa
     2581 acagcttctc aaaacggttt tgattatcca gccagccaat atatttgtta tatgtcttat
     2641 cgctaatcat ggaaagtatg aatggctttc cataacgacg ccctagcaga aaaatgataa
     2701 tcgaaccaat gcagattcca atatagttat agataaatcc agcataaggg ccaaatatca
     2761 ggacaccagc tgcagtactg atccctccag gaatgattgg aataactact tgcaggattt
     2821 ggatgaagac aaaaataatc ggtcctaaaa tgatggaatc acctactaat cctcttaacg
     2881 cattcaaatc tttgaaaaca cctaaattaa taaaataaat cgtcagcgcg attgataggc
     2941 ctaatccgat aatggatatg aaatttatca gttttcgaga ggcagcaata ttcattattc
     3001 atcaactcct tagaggttct ttgttaacta tactttacta gaaaagcaac taactgcgcc
     3061 attttttgat atgacagaaa acaaacaatt ttgtaagttc atcataagta taaaagattg
     3121 atctcaaaaa aacatccaac taaagactta tttaataact tgttttttt
//
LOCUS       DACDKL010000219         3116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDKL010000219 DACDKL010000000
VERSION     DACDKL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.222"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IV329_002517"
     CDS             complement(<1..2232)
                     /locus_tag="IV329_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941670.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3116)
                     /locus_tag="IV329_002518"
                     /pseudo
     CDS             complement(2904..3116)
                     /locus_tag="IV329_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          861 a          703 c          513 g         1039 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaata
//
LOCUS       DACDKL010000220         3097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDKL010000220 DACDKL010000000
VERSION     DACDKL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.223"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(86..1615)
                     /gene="ahpF"
                     /locus_tag="IV329_002519"
     CDS             complement(86..1615)
                     /gene="ahpF"
                     /locus_tag="IV329_002519"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8941671.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1625..2188)
                     /gene="ahpC"
                     /locus_tag="IV329_002520"
     CDS             complement(1625..2188)
                     /gene="ahpC"
                     /locus_tag="IV329_002520"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8941672.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2332..2994)
                     /locus_tag="IV329_002521"
     CDS             complement(2332..2994)
                     /locus_tag="IV329_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8941673.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT          911 a          634 c          535 g         1017 t
ORIGIN      
        1 ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc
       61 tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag
      121 ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat
      181 caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat
      241 ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta
      301 gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg
      361 agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag
      421 tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt aattctggta
      481 agaattctag aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac
      541 cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt
      601 gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac
      661 gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta
      721 agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa
      781 tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg
      841 tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag
      901 aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag
      961 ctgctggtcc accgccaatt actaatacat cataaggttc tttttcaaac aactcttctt
     1021 caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa
     1081 attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt
     1141 tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata
     1201 tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa
     1261 aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg
     1321 cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga
     1381 taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc
     1441 gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa
     1501 attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag
     1561 attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca
     1621 ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc
     1681 attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat
     1741 caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga
     1801 ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa
     1861 catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac
     1921 cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag
     1981 aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt
     2041 cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca
     2101 taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt
     2161 cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat
     2221 aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat
     2281 tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg
     2341 gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg
     2401 caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc
     2461 ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg
     2521 gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc
     2581 aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt
     2641 tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt
     2701 gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg
     2761 agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt
     2821 atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat
     2881 agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata
     2941 gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat
     3001 tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt
     3061 atgtcacatt aactagtaaa tttgtataaa tagtaaa
//
LOCUS       DACDKL010000221         3082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDKL010000221 DACDKL010000000
VERSION     DACDKL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.224"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            429..1340
                     /locus_tag="IV329_002522"
     CDS             429..1340
                     /locus_tag="IV329_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941674.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1827..2093
                     /locus_tag="IV329_002523"
     CDS             1827..2093
                     /locus_tag="IV329_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941675.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2165..2506
                     /locus_tag="IV329_002524"
     CDS             2165..2506
                     /locus_tag="IV329_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941676.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2561..2980
                     /gene="ssb"
                     /locus_tag="IV329_002525"
     CDS             2561..2980
                     /gene="ssb"
                     /locus_tag="IV329_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8941677.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
BASE COUNT         1114 a          502 c          669 g          797 t
ORIGIN      
        1 gggaaaaaat caacccctac cccagtcttg gggtctcaaa actaactccc ccctaacccc
       61 ctctttcagc ctcttttgaa agagggggag aatcaacccc acggccaacg ccccagtgtt
      121 cttggggctg tccagaacag aaacttgcga gagagtgggg gtaaaacaag tgtagaggaa
      181 acttgaagac ttgttcgaac agaaacttgc gagagagccg gagtaaaaca agtgtagagg
      241 aaacttgaag acttgttcga acagaaactt gcgagagagt tggagtaaaa caagtgtaga
      301 ggaaacttga agacttgttc gaacacaaac ttacaagaga ggggagtaaa gcagttgcag
      361 aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa agcattatta aattgaaggt
      421 gatgaaatat gaagtattta ttgcatggct attttattga ttattctact cgcaatagta
      481 gtactttagc gattctatgt ttgcttcatg aattgagagt ggttactaga aaacagctat
      541 acaaactatt gaattttgac agatcaatta gtaaacaagg agtaacgaat gctttagtga
      601 gattatatga aaaacagtta atcgatcgaa aaaaatatgg aacagaaatg tattattatt
      661 tgacgaaaga gggacatcaa agcataggtg ggtattatac gttgccgaaa gttcctgaat
      721 ataatttgaa tcaccattta caaattaaca attatttgat taaaggaatg gcattggcaa
      781 aagaccaccc acatttaaaa cggattattt cagaaagacg taccgtctat gaaacgaagg
      841 atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc agattttatt tttcgttttc
      901 gagacaaaga aaatcatgag atcaattggt cctttgaaat tgagctaacg ttgaaaacac
      961 gtcgtcgcta ccgtgaagcg atttttccga aatatattcg tgagttgcga acacgtcctg
     1021 aatcacattt aatttatgtg acacctagtg gcgcaattca agaagaactt catgcgttta
     1081 agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag tcaacaagaa ttgtttgagc
     1141 ggttgcatat tttttcagct ttagagtttg aagagggatt aaaagcatta ttagagaatg
     1201 atccagcagt caattggcgg acagtagaac gagaaaaaat ttatccgaat ggcgctgttc
     1261 aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga ggaaaaacca aaagtgaagt
     1321 ttacgcctga agtattgtaa aagagggtgt tcgcttcgct ctttttttgt gccagtttgc
     1381 tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt taggaaggac taacttgttt
     1441 gatttatccg ttgtcaaaga agtggacgtt cgagagtccc taatcgtata gcagtcattc
     1501 ggcgagtata acctaaaaca taacctgcca attgccaagg gtaaaatgca cgctgcgcgc
     1561 ccttggcaat cgtcagaaca tgttttcgag ccggttatgc cgaacgaccg ctatacgaaa
     1621 agggcctcta aacagaagtc cactttgatc cttgacaact gaaacccaaa atcaaacaag
     1681 caggccttcg tctaaaaacg agccgaatga agaccttggg ggttgcaccc ccaaaccccc
     1741 gtgcccccga cacactaccg aaaaggtggg ggtgggtggc gaaaaaatca aataaaacat
     1801 tctattcaag aaaggaagaa acaaccatga caactaaaaa cgagcaactg aaagaacagg
     1861 caagaaaaga gttgcaagaa agaggtttga ttattgaggg gagctttgaa ggagattttg
     1921 aaacctatat tggttgttat gcaagaccga taaacaaacc tacagcctta gacccaatga
     1981 acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa tggttttccg caagatttca
     2041 ctgaatggta tgagtgggaa attaacaacg ggaagcttga aaacttctta taaaagtcag
     2101 atgacgtgca aagagaatga ttctctttgt acggtaaaaa tataaagaaa gaaggaaaaa
     2161 gaaaatggca aaaaacacac cttatcaagt aggagacatt ttttatagta gttggggcta
     2221 cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta acagaaaaaa cagcatggtt
     2281 tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc atgagtggaa aagttgttcc
     2341 aatgaaagac aagtttatag agtatccttt agcaaaaact aaagacagta ttgtaaaaaa
     2401 acgaattgat ccagaaacct atcctgatat gttatgtggt tatcgtagtt gggacacgtt
     2461 tcatagatat aaaaatcaag ctgtttatga aagtagttat tattaagggg atagtttccc
     2521 ctttcaaaaa taataaatag aagaatggag agaaaaaatc atgatgaatt cagtgaattt
     2581 agtaggacgg ttaacaaaag aagtggattt aaaatacaca ccaaaaggca atgcgacagg
     2641 cacatttatt ttagctgtta atcgaaatta tacgaatgca aacggcgagc gtgaagcaga
     2701 ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact ttagcaaaat atactggaaa
     2761 aggaagtttg attggcatta atggacgtct tcaaacgagg agctatcaga atcagcaagg
     2821 acagaccatg tatgttacgg aagttctagt gacagatttc tatttgttag agagtaaaga
     2881 aatcaatgag caacggtcaa aatcaatggc gaataatcaa aataattctt caattccagg
     2941 aaatgagatt caaatttcag atagttattt gcccttttaa gtgaaaaata attttgttct
     3001 atacctgatt tcttcaggta tagaacaaaa aataaataga gatgaggcga ttttttatga
     3061 aaatcattga cagttacgat ca
//
LOCUS       DACDKL010000222         3056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDKL010000222 DACDKL010000000
VERSION     DACDKL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.225"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..114)
                     /locus_tag="IV329_002526"
     CDS             complement(<1..114)
                     /locus_tag="IV329_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8941678.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
     gene            991..1257
                     /locus_tag="IV329_002527"
     CDS             991..1257
                     /locus_tag="IV329_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941679.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1315..1827
                     /locus_tag="IV329_002528"
     CDS             1315..1827
                     /locus_tag="IV329_002528"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8941680.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1824..2861
                     /locus_tag="IV329_002529"
     CDS             1824..2861
                     /locus_tag="IV329_002529"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8941681.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1095 a          542 c          596 g          823 t
ORIGIN      
        1 acgatcatat aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaacc
       61 aacagcgaca ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct
      121 gtcctctctg tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa
      181 cagaaccgtt ttattgatga ccacgaaagt gcatggcgat atttgatgtt gatactttat
      241 gcagtaccct atgtattctt cgctatcatt ctgtggctca tagcattgtt ttttagagta
      301 tttaatgacc tttaggtaga tttatatata aaaagggcta aatacgcaaa ataccccctt
      361 aaaatgcaaa ataaatgcaa ataagggggt aactccttat gaaaaaacaa ctaatagtat
      421 tataacacgt ttttatgccg agaaaactta ttggttggaa tgggctatgt gttagctaac
      481 ttgttagcga gttggttgga cttgaattgg gattaatccc aagaaagtac caactcaaca
      541 acacataaag ccctgtaggt tccgaccaat aaggaaattg gaataaagca acgaaaggag
      601 ttgagtaaaa tgagtgagaa aacgaagaaa atcattcgag tatcattagc tttttcacaa
      661 gtagtagtgc aatcattgat agtttattat acttaccagc aatataaatt atctaaagag
      721 aattaagttt tgattatgta ataagttttg gtactaaaaa agacttgatc taattagacc
      781 aagtcttttg ttagtgttat attaataaca aaataaaaat aagaagtcgc tcactccccg
      841 accaaagttt gtgagcaaaa taaacagtcg atgtttgttt cttaattgta tatcttgata
      901 ttaaacgatt taagattatt cttcaagata tatattcgca aatgtaattg gaaaagcgac
      961 ttcttaaaca aaattaagga gtcgcttttt atggcaaaaa taacgaaaaa tgagcaagaa
     1021 aatttggtgg aaaattacac aaaaaaaccg gcgtactcta atagccggtt agacgcacat
     1081 ttcgtgtgca cttctaatcc gaaattgagc tttgacgcta tgacgatagt cggaaacctt
     1141 aataaaaaca gcgctaaaaa gctatccgat tttatgagtt tagatccaca aattagactt
     1201 tgggatatac ttcaaacaaa atttaaagct aaagcactac aagaccccct atgttaggct
     1261 agttgtcgta taggataata agttataata aagactatac ggaggatgat taccatgaca
     1321 aataaaaaca atgaacgtta ctctaccgaa ctgaaagcga cagccatcaa gaaaatgatg
     1381 ccacccgaaa atagggggat tcgccaatta agtcaaactt tgggcattcc agaagcgact
     1441 ctttattctt ggcgtaaaaa agctagaatt caagggaata ctacacccgg aacgaaaaaa
     1501 aatgtcgaaa aatggtcaag tgaagataaa tttttaattg tgatggaatc attctcaatg
     1561 aaccaagtag aacttgcgga atattgccga aagaagggct tatttaaaga acaaattgag
     1621 gcttggaaga aaacgtgctt atctgcgaat gaacaggaag aaaatcgaac gagagaactt
     1681 gcaactgaac taaaagaaga gaaaaaacaa gcgcgtcaat tagagaaaga attacgtcat
     1741 aaggaaaaag cattagcgga agccgctgct ctcttattac taagaaaaaa agcacacgcg
     1801 atttgggggg accaagagga agaatgattt catcttcaga tcgcaaacaa gctgttgaac
     1861 ttattcaaga agccaatcgc aatggggccc gtctcaccta tgcctgtaat gagctgaata
     1921 ttagcgttcg aacctacgaa cgatggacgc gtgaaggtac gatagcgcat gatcaacgcc
     1981 ctttggcgaa acgtcctgta cccaaaaata aactgacgga tcaagaacgc gaaaaaataa
     2041 tagaaaccgt ttcaaaaaaa gaatttatga atttgccacc tagccaaatt gtaccaaaac
     2101 ttgcggattg ctcgatttat attgcatcag agtctagctt ctatcgtgta ttgcgagaaa
     2161 aaaatatgca acatcaccgt ggccgaagtc aagtttcaca aaaacgcata ccgccaagtc
     2221 accttgcgac gaagccaaat gaagtttgga cttgggatat tacttggcta aaaggtccaa
     2281 taaaaggact cttttaccgg ttatatctca tcattgattt atttagccgt aaaatggtag
     2341 gctgggagat ttgggaatca gaagaagctc gtcatgccga aaaacttatt aaacagacgg
     2401 tgcttaagga acgtatccaa ggacagccgt tggttttaca ctccgataat ggcagcccta
     2461 tgaaagccgc gacttttcaa gtcttactcg aaaaattagg aattcaaagt tcttattcaa
     2521 gaccacgagt gagtaatgac aatccttatt cggaagcggc tttccgaacg ttaaaatatc
     2581 gaccagaata tcccaataac ggctttaaaa caatagatga tgctcgaaaa tggacgaaag
     2641 agtttgtcga ttggtataac gattctcatt tgcatagtgg gattcaattt gtgtctcctg
     2701 cagcgtgtca tgaaggacga catctcgcta ttttagagaa acgaaagcag gtttacgagg
     2761 acgctaaaaa gcgccatcct gaacgctggt caaaaggtgt tcgaaattgg actccacatc
     2821 aatcggtggc gttgaatcct ttaaaagaaa cagaaagtta acaggagcat aatctttcaa
     2881 gattatcaaa taaccctcgt cacccaatag gggaaaaagt tattcttccc ctattggata
     2941 acgaggccaa ccgaccatag ggaggttctg ctttttgata ggtggaaaat aaaaatacga
     3001 caactatatt gacaaacacc gaccatcgtc cagtaaagag acgcaataaa ttctat
//
LOCUS       DACDKL010000223         3045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDKL010000223 DACDKL010000000
VERSION     DACDKL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.226"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(183..1913)
                     /gene="spxB"
                     /locus_tag="IV329_002530"
     CDS             complement(183..1913)
                     /gene="spxB"
                     /locus_tag="IV329_002530"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8941682.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            2078..2896
                     /locus_tag="IV329_002531"
     CDS             2078..2896
                     /locus_tag="IV329_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8941683.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
BASE COUNT          858 a          584 c          631 g          972 t
ORIGIN      
        1 aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc
       61 ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat
      121 gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt
      181 tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac ttcatatttt
      241 tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct
      301 actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt
      361 gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc
      421 ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt
      481 tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca
      541 ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac
      601 ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt
      661 cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta
      721 ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa
      781 atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt
      841 tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga
      901 tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa
      961 gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac
     1021 gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg
     1081 aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta
     1141 gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt
     1201 gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga
     1261 aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa
     1321 ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga
     1381 gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg
     1441 atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga
     1501 ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca
     1561 tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct
     1621 actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca
     1681 ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct
     1741 gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga
     1801 tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata
     1861 ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt
     1921 cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc
     1981 gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta
     2041 gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact
     2101 tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg
     2161 tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg
     2221 atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac
     2281 aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg
     2341 cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca
     2401 tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac
     2461 attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc
     2521 ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca
     2581 aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa
     2641 aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg
     2701 agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg
     2761 agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag
     2821 caaacgtcat tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga
     2881 tgtggcatta ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta
     2941 tatcgcgacc ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt
     3001 aataatagtt ttttctatgt gtatttcctt tgatccagcg aacaa
//
LOCUS       DACDKL010000224         3033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDKL010000224 DACDKL010000000
VERSION     DACDKL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.227"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            102..2543
                     /locus_tag="IV329_002532"
     CDS             102..2543
                     /locus_tag="IV329_002532"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8941684.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1001 a          522 c          693 g          817 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cat
//
LOCUS       DACDKL010000225         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDKL010000225 DACDKL010000000
VERSION     DACDKL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.228"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IV329_002533"
     CDS             177..359
                     /locus_tag="IV329_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP8941685.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IV329_002534"
     CDS             462..746
                     /locus_tag="IV329_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941686.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IV329_002535"
     CDS             762..998
                     /locus_tag="IV329_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941687.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IV329_002536"
     CDS             1020..1274
                     /locus_tag="IV329_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941688.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IV329_002537"
     CDS             1356..1541
                     /locus_tag="IV329_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8941689.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IV329_002538"
     CDS             1608..2003
                     /locus_tag="IV329_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8941690.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IV329_002539"
     CDS             1993..2370
                     /locus_tag="IV329_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941691.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IV329_002540"
     CDS             2371..2766
                     /locus_tag="IV329_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941692.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDKL010000226         3007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDKL010000226 DACDKL010000000
VERSION     DACDKL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.229"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(276..692)
                     /locus_tag="IV329_002541"
     CDS             complement(276..692)
                     /locus_tag="IV329_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941693.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="IV329_002542"
     CDS             complement(695..979)
                     /locus_tag="IV329_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941694.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="IV329_002543"
     CDS             complement(1679..1774)
                     /locus_tag="IV329_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8941695.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="IV329_002544"
     CDS             2427..>3007
                     /locus_tag="IV329_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8941696.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          957 a          478 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DACDKL010000227         2998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDKL010000227 DACDKL010000000
VERSION     DACDKL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.230"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(552..2165)
                     /locus_tag="IV329_002545"
     CDS             complement(552..2165)
                     /locus_tag="IV329_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8941697.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..>2998)
                     /locus_tag="IV329_002546"
     CDS             complement(2306..>2998)
                     /locus_tag="IV329_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941698.1"
                     /translation="STQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKN
                     EQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQ
                     ELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASL
                     KKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYT
                     GLSPSKYYLSLK"
BASE COUNT          965 a          531 c          522 g          980 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtaga
//
LOCUS       DACDKL010000228         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDKL010000228 DACDKL010000000
VERSION     DACDKL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.231"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(48..788)
                     /gene="truA"
                     /locus_tag="IV329_002547"
     CDS             complement(48..788)
                     /gene="truA"
                     /locus_tag="IV329_002547"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8941699.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(979..1614)
                     /locus_tag="IV329_002548"
     CDS             complement(979..1614)
                     /locus_tag="IV329_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8941700.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1761..2981)
                     /gene="sstT"
                     /locus_tag="IV329_002549"
     CDS             complement(1761..2981)
                     /gene="sstT"
                     /locus_tag="IV329_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8941701.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
BASE COUNT         1014 a          647 c          478 g          853 t
ORIGIN      
        1 gatatttata ctgtgaaaca tgcgtttcat ccattatcaa agtattttta tcgataataa
       61 acttcatcca aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg ccgcactttg
      121 ctttcaaata tttcatcgat tgtttctatc ggcatggcac ctgtgccgat ttctagtaat
      181 gtccccatga taatccgcac cattttatat aagaatccgt ctccgacaaa agtaaaatga
      241 agcatttctc cctttcgttc gatagataat tcatcaatcg tacgcactgt ggattttttt
      301 gattttttca atgcagaaaa accaatgaaa tcatgtgtac ccactaattt ttcgcaagct
      361 gcattcagct tgtccatatc caactcttga gggaaataaa agctgtggtt tcgctcaaat
      421 gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt ttttgcccac cgcattatat
      481 cttgaatgga agcgctcagg catttcttcg attcttttga tgacaatatc ccgcggaagg
      541 taacgattca taaagtcgat catttctgcc agtccaattt ctgtgtttgt tttaaaattt
      601 gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat aatttctatc
      661 tcttcatttg tcatctgatg caatactttt tcgatttttc cttgaatggt tttatcgtca
      721 tctccaagtc tttgccatcc tgaatatctt tttccgtcat attcgattgt caacttgatg
      781 tttctcatat tttagtggct cctctgtctt ccatttggtc ttctctctac ctttttaaga
      841 ttgtagcaca aaatatagct ccttgcttct ctaagacaga agctatacaa gaaaactgtg
      901 gaaatatata tttcagaaaa acaaaaaaca gccaccggat gaagtatccg ctgactgttc
      961 aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaacctgg caatgggcac
     1021 ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta atggccaaga
     1081 atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac tgatcacaga
     1141 cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga tcaagaaaag
     1201 aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat tatggaccca
     1261 tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg taaagataca
     1321 atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca cacgagatgt
     1381 actaacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc ctgaaaccac
     1441 agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata cactgatcac
     1501 gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag tcaaaaaatc
     1561 actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat ccatgatacg
     1621 cctccaataa caattaccta tgcttttaat tgttattata aacgacttta tcttgattta
     1681 acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga atcattttaa
     1741 aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc tttcaatttg
     1801 attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa caacagatca
     1861 cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat aaatccaata
     1921 ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc taatggaatc
     1981 aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc agacaaaata
     2041 cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac agcagtcaac
     2101 gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc taacgggatc
     2161 gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat gtttatcggg
     2221 atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt caaacaaaag
     2281 aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg gtacacaact
     2341 aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata ctctgccaat
     2401 ccagcgattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc tatcggagca
     2461 aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc caccaactct
     2521 tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca gaataagacg
     2581 ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt cgtcaacata
     2641 gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc ttgtaaagta
     2701 ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag caatgttccc
     2761 actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt atgcttggca
     2821 attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa gcaactgaca
     2881 aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc aaacaatatt
     2941 ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa ct
//
LOCUS       DACDKL010000229         2987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDKL010000229 DACDKL010000000
VERSION     DACDKL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.232"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            10..1662
                     /gene="alsS"
                     /locus_tag="IV329_002550"
     CDS             10..1662
                     /gene="alsS"
                     /locus_tag="IV329_002550"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8941702.1"
                     /translation="MKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGPELIV
                     TRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAGQVKR
                     SDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFISIPQ
                     DVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKETLAI
                     RQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAIGYDP
                     IEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQQVSE
                     DAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSHYIWM
                     ARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQDLET
                     AVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGIRATS
                     VEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1675..2385
                     /gene="budA"
                     /locus_tag="IV329_002551"
     CDS             1675..2385
                     /gene="budA"
                     /locus_tag="IV329_002551"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8941703.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2515..>2987
                     /locus_tag="IV329_002552"
     CDS             2515..>2987
                     /locus_tag="IV329_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8941704.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNI"
BASE COUNT          994 a          502 c          678 g          813 t
ORIGIN      
        1 gctgaaaaaa tgaaacaagg ttcgaaatct gtagtagaaa gtttgattaa tcatcatgtt
       61 gattatgtat tcggtatacc aggtgcaaaa atcgatggtg tatttaatga attagaggat
      121 caaggtcctg aattaatcgt gactcgtcac gaacaaaatg ctgcatttat ggcacaagca
      181 atcggacgta ttacaggaga gccaggagta gttatcgcaa caagtggtcc tggtgctagt
      241 aacttagcta caggtttggt aacagcgaca gcagaaggtg acccagtttt agcaattgcc
      301 ggacaagtaa aacgaagcga tctattgaag ttgacccatc aaagcatgga taatgcagca
      361 ctgttccagc caatcacaaa atacagtgca gaaatccaag atccagaaac aatttcagaa
      421 gtgattgcca atgcttatcg tatggctaag agttcaaaaa aaggcgcgag ctttatcagt
      481 atcccgcaag atgtagtgga tgcacctgtc caaggtaatg tcatcaagcc attgagcgat
      541 cctaaattag gttctgcttc tgcagatgat atccgttacc tagctgaacg cattagagaa
      601 gcaaaactac cggtactatt agtaggtatg cgaggatcaa gtgaaaaaga aacgttagcc
      661 atcaggcaat tagttgaaaa gacagcattg cctgttgtag agacattcca agcagctgga
      721 gtgatctcac gtgaattaga agcgcatttc tttggtcgtg tgggtttatt tagaaaccaa
      781 ccaggagata tgcttttgaa acgaagtgat ctagttattg caattggcta tgatccaatc
      841 gaatatgaag caagaaactg gaatgcggaa aaagatgcgc ggatcattgt gatcgatgaa
      901 gcgccagctg aaatcgatcc ttttatgcag cctgaaagag aattgatcgg tgatatctca
      961 gccactcttg atttattgac aggttcatta gagcctcaac aagtgtcaga agatgcaaaa
     1021 gaatatttag catctcttca agcaaaatta acggagcggg acattgttca atcaaaagga
     1081 gaagcaggta tcttgcatcc tttagaagtt attaacacgc ttcagtcaaa agtaacagat
     1141 gatatgactg ttaccgtaga tgtaggtagt cattatattt ggatggcacg ccactttaga
     1201 agttatgaac ctcgtcattt gttattcagt aatggtatgc aaacgcttgg tgttgcattg
     1261 ccatgggcga tttctgctgc attggtgcgt ccaaatacac aaatcgtatc agtttccggc
     1321 gatgggggat ttttattctc tgcccaggat ttagaaacgg cggtacgtaa aaaattgaat
     1381 atcgttcatt tgatttggaa tgatggacac tacaatatgg tagagttcca agaaaaaatg
     1441 aaatatcagc gggcgtcagg tgtagacttt ggtccagttg attttgtgaa atatgcagaa
     1501 gcttttggcg ctaaaggaat ccgagctaca agtgtagagg aattagaaaa agctttagaa
     1561 gaaggatttg cgacagaagg acccgtaatt attgatattc cgattgatta ccgtgataat
     1621 gagaaattgg gagaaacaat cttaccagat caattctatt aaggaagtgt cgacatgact
     1681 gaaaaaatct tataccagca cgggacatta ggagctttga tggccggctt gatggatgga
     1741 acagaaacga ttgctcatat attagaaaaa ggaactctgg gattgggaac attgcatggg
     1801 cttgatggtg aagtcatctt tttagatggc gtagcttatc aaggaagatc agatggctca
     1861 gttgcccaat tagatggaag tgaacttacc ccctatgcag caattacaga ttttacgcca
     1921 gatactagtt tttctgtctc aaaaactgca gatggagaac aattgaaaaa agacatatta
     1981 gtaagagaag ccggggaaaa cctatttttg gcagtgaaga taactggatt attcaaaaat
     2041 atgcacatcc gtatcatgcc gaagcaagaa aaaccttaca gaagactagc aaaaatttca
     2101 gagtctcaac cagaattcca acaaagcaat atccaaggaa cattagtcgg gttctttacg
     2161 cctgaattat tccaaggagt tgctgcagcg ggatttcacc tgcattttat cgatgataca
     2221 catacatttg gcggacatgt aatggatttt gaagtggctg aaggaaaagt tgagattagc
     2281 cgcatctcgt cccttgtaca gcattttcca gttgatgatc cgacttttgt tcacgaaacg
     2341 attgattatg ctgatttagc agcagaaatc caacaagcag aatagaaaaa gacagcgaga
     2401 tgcgttcaag gtgattaacc tgacgtatct cgtttttttg aggaggaaac ctagcgttta
     2461 caaaaaaact gagctataat cttctccata gatcaaaaag gagcggtttt tatcatgtgg
     2521 acagaaaaac agaaaaaaga tgcatacgaa attttattat tacagcaaca aggattatta
     2581 gaagcagaag ataactggtt agcaaattta gcaaattcat ctgcattgtt gaatgagact
     2641 ttgcctgaga ctgtctttgc aggatattat ttattcgatg gtaatgaatt gattttagga
     2701 ccgttccaag ggcgagtttc ttgcacacgt atcaagatgg gaaaaggagt ttgtggagaa
     2761 tcagctgaaa aacaagaaac attgatcgtt gatgatgtca aaacccatgc aaattacatt
     2821 tcctgcgatt cagccgcaca atcagaaatc gtagtgccaa tggtaaaaga aggtaattta
     2881 atcggtgttt tagatatcga ttgcggagta acgaaagggt atgatgaaat cgatcaagca
     2941 tttctagaaa aatatgtaga tcttttatta gcaaatttta atataaa
//
LOCUS       DACDKL010000230         2978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDKL010000230 DACDKL010000000
VERSION     DACDKL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.233"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            152..919
                     /locus_tag="IV329_002553"
     CDS             152..919
                     /locus_tag="IV329_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8941705.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            909..2951
                     /locus_tag="IV329_002554"
     CDS             909..2951
                     /locus_tag="IV329_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8941706.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          960 a          481 c          597 g          940 t
ORIGIN      
        1 tttttgcata tttaattttg ctgaataaaa taattgaata aaatttttta ttcagtccaa
       61 gtaaaagtga caaaaacata agagcaaatt attcgtagat atgctactat gcaaataaat
      121 agaaatgaaa aatgaaagag gaggagaaga attggaaaag atgatcgaag tgaaacatct
      181 aagtaaaagc tacggcagca aactttctcc cgtcaaagcc ttagataata tctcattcac
      241 agtagaagaa ggcgagtttg ttgggattat gggcccaagt ggtgcaggaa agacaacgct
      301 gatgaatatc ttgtccacga tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa
      361 agaaatcaca aaaatgaaaa acagtgagct cagtgacttt cgtcgaaaaa aactaggatt
      421 catttttcaa gagttcaatt tgatcgatac cctaaatgca aaagacaata tcctattgcc
      481 cctagcagtt gaacggatga ctaaagagga aatggaaaag agagtaagac atgtagctca
      541 attgttgaat atagaagaac tgctcaaacg gtatcccgat gaactttctg tcggacagag
      601 acaacgaatt gcagctgcta gagcacttgt tgttcagcca caagtgattt ttgcagatga
      661 accaactggc tctttggact cgaagtcggc tacagaattg ttgaactact tgaagaccat
      721 gaatcaaaaa gaaaaagcaa cgatactgtt agtcacccat gatccatata cagcaagcta
      781 ctgcgataga attttattta ttaaagatgg tgtgattttt tctgaagttg tcagaagagg
      841 gacaagaaga gagttctttg agaaagtaat agacatgcag gcgacgattg gcggaggagg
      901 aaaaatgaat gctgtataaa ctagccgtta aaagtttttt tcggcagtca cgaggctatc
      961 ttgtttattt tttcagtttg actttatccg taatgatcta ttattccttc agcgcaatga
     1021 cctacgatca atctttagtc agaagagcaa gtcaagatac ttcgatagat ggtggattga
     1081 gttttggtgg atttatcatt actgttgttt tgcttttttt cgtattcagt gccaaccgat
     1141 ttttcttgaa tagaaggcaa aaggaaatag gcatctatca gctttttggt atgaataaac
     1201 tgcagatatc aggtatatat gtgttagaaa tcatgataat tggattgttc gcctgtattt
     1261 ctgggatatt actcggtatt attttttcca aactcttttc aatgattttg gttcgaatga
     1321 tggatatgga tcttactagt ccatttttta tctccattcc atcagtagta gatactttat
     1381 ttgctttttt catcatcctt ttagcagtat ctgtatattc aatatggaaa atatggcgct
     1441 atccaatgat tcgaagtttt ggagaaaaag aacaagcaga tagttcaaca atgcgcttca
     1501 ggacaaggca ccgattactc gcagtcatcg ggttactact tttaacgagc ggatattttg
     1561 gagctagtca ttttagggaa atcacttcat ggatgatcag ttatcattat cttgatacat
     1621 tagttttctt ttttcctttt ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt
     1681 tctgttatac atttcggtta tttttgatca tattgagtaa atggaagatt tattactatg
     1741 gtatcaattt tttgataatg ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa
     1801 attctctgat tacagtcatt ctcggaattt ctttaatgac aattggcggc acggctagta
     1861 tctctactat tttatcctac caggagaaag tttcctcccc gatggactat cagtttgatg
     1921 ttgagacaga gaaaaaaatc aagccgattc tagcagagga agggcagaag ataactaaag
     1981 aaatcacctt gcattataaa gtcattggga gtatctatag tcaaaatctc gaagaaccta
     2041 tgtttcaaac tgctaatctg atcacggaaa cacaatatca agccttcaga aaagttaatc
     2101 cagatttgcc gaacatttct ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc
     2161 aaactctttt tcggaatatt tcattatatg gatctagtat tggactgcca gataatcaag
     2221 aattgaagat tcaacaaatg cttccaagtg tattaggtga cgaaacaatg gtatatctaa
     2281 gtcctgtact gattgtagat gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag
     2341 tagtcaatgt agatgtatca ggtggaaata atgaaaaaat agatgaacga ctgagcaatg
     2401 acctgtcgat aaagtggctc aatgctgttt attattctta tgatatcgta aatggaagtc
     2461 tacaaggaga aattagcaca aagaaacttc cggatgacca aatgaaggaa gcacaattta
     2521 gccaagaaag ctctcgtttg aatttctctt ccagctattc gaaattgcgt gcagttaatc
     2581 ggcgaatcgg tatcaatatt tttgtcacac tatttgtcgg gatgatcgtg attattgcta
     2641 ctggtagtat tttgacagtc cggcaattag cagaagcaga ggaagaaaaa gaaaattatc
     2701 acctattgac taagctgggg attccacaaa gaaggatcag acggatgatc ttcgagcaaa
     2761 acgcacttac cttctttcca cccatgatat tagggatcac tcacgcagta tttgcaatca
     2821 atgtcttcac tcagtatata aaaactgccg attactggtt ggcttatctt gtgagtggct
     2881 tattgatcct tatctatctt ttgctttatg tcattacgag tcagttatat tgtcggatta
     2941 tcgaggaatg atatacggaa taagacaaga taataaat
//
LOCUS       DACDKL010000231         2962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDKL010000231 DACDKL010000000
VERSION     DACDKL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.234"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(72..896)
                     /gene="nadE"
                     /locus_tag="IV329_002555"
     CDS             complement(72..896)
                     /gene="nadE"
                     /locus_tag="IV329_002555"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8941707.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(908..2380)
                     /locus_tag="IV329_002556"
     CDS             complement(908..2380)
                     /locus_tag="IV329_002556"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8941708.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT          817 a          630 c          496 g         1019 t
ORIGIN      
        1 atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt
       61 ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt
      121 tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct
      181 tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt
      241 ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc
      301 aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg
      361 gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc
      421 cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt
      481 gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt
      541 acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag
      601 tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg
      661 ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc
      721 aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga
      781 aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt
      841 ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat
      901 tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag
      961 ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt
     1021 ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt
     1081 ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc
     1141 gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga
     1201 tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc
     1261 catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg
     1321 cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta
     1381 gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac
     1441 cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt
     1501 cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac
     1561 gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt
     1621 cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag
     1681 tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat
     1741 catttccata agattggact aacgaatgag catgggtccc gctagctggt atcccaaata
     1801 tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc
     1861 cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt
     1921 ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca
     1981 tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatca
     2041 atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt
     2101 caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag
     2161 caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg
     2221 caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat
     2281 gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg
     2341 tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc
     2401 aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt
     2461 ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta
     2521 aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat
     2581 gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga
     2641 ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat aaccaaatgt
     2701 ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt ccttttttgc
     2761 ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt ggaaaggcga
     2821 attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca cacgttttta
     2881 aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg gcgagacatc
     2941 aatcagtctc gccttgtttt tt
//
LOCUS       DACDKL010000232         2947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDKL010000232 DACDKL010000000
VERSION     DACDKL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.235"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(449..865)
                     /locus_tag="IV329_002557"
     CDS             complement(449..865)
                     /locus_tag="IV329_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941709.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IV329_002558"
     CDS             complement(867..1148)
                     /locus_tag="IV329_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941710.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IV329_002559"
     CDS             1948..2922
                     /locus_tag="IV329_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8941711.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACDKL010000233         2900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDKL010000233 DACDKL010000000
VERSION     DACDKL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.237"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..141
                     /locus_tag="IV329_002560"
     CDS             <1..141
                     /locus_tag="IV329_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HAP8941712.1"
                     /translation="PSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRYFSLREEGMME
                     EK"
     gene            291..491
                     /locus_tag="IV329_002561"
     CDS             291..491
                     /locus_tag="IV329_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8941713.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(745..2424)
                     /locus_tag="IV329_002562"
     CDS             complement(745..2424)
                     /locus_tag="IV329_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8941714.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(2426..2638)
                     /locus_tag="IV329_002563"
     CDS             complement(2426..2638)
                     /locus_tag="IV329_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8941715.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT          862 a          566 c          580 g          892 t
ORIGIN      
        1 ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa agtgtggaga aatgatgggg
       61 atcactgtac tggaccactt gatcatcgga cgcaaacgat attttagttt gagagaagaa
      121 ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa gtgtacaatt
      181 atattgtaat tttgtttggg aatagaagtt gaaacatctg ttgcacctct atcgtacacg
      241 agcaatgtta caatttaaat tagtgctttt ggcacgagga ggatattatt atggaaaacg
      301 gaacagtaaa atggttcaac gctgaaaaag gatttggatt tatctcacgt gaagatggaa
      361 gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg attcaaaaca ttagaagaag
      421 gtcaagcagt gactttcgac gttgaagaat cagaccgcgg cccacaagct gcaaacgttg
      481 taaaagctta attttatctg taaatcaaat tgatggtaga catcaatttt tacgagagaa
      541 caaagcgagt aacaaaaata cctgccacaa gagacaattt ctcttgtggc aggtattttt
      601 ttgtatgaaa aagtaaataa atttaagagg ctatgaaaga gccaccttgt atcaaggaat
      661 aagaacaaac aaaaatggct tctcatgttt ttcatttgtt ttctcttata ttcaggacaa
      721 ggtgctgatt tcataacctc ttatttattc ttcatctggt gtcattacca ttggcaaaat
      781 cattggatgt ctttctgtac gttcgaataa gaaaggctga agcgcgccag tcatcgcttc
      841 tttcaattta tattctgaac agtcaggtcc attcaaacat tctcttaaag cgtggaataa
      901 gattcgctga gcttcattga tcatttctcc tgattctcgc atgtagacaa atccacgaga
      961 gagaatatct ggaccagcca agatttcttt tttcttgata tccactgtcg cgacagctaa
     1021 tacaagacct tcttctgaaa gcaggtgacg gtcacgtaaa acgacattac caatatcacc
     1081 gatcccgcta ccatcgatat acacatctcc agccacaaaa tgcccagcag gacgcgcaga
     1141 atcgctagtt aacgctaaaa catcgccatt ttccatgatg aagcagtttt ctgcaggaac
     1201 acctacatct tgtgctagag aagcatgtac tttcaacatg cggaattctc cgtggacagg
     1261 aataaaatat tttggcttca tcaaacgaag cattaatttt tgttcttctt gtccaccgtg
     1321 accggaagta tggatgttat tgatttttcc gtgaatcact tctgcaccag cttcagacag
     1381 caaattgatc aaatgattga cacttgttgt attaccagga ataggtgaac ttgaaaagat
     1441 cactgtatcg tctggctgga tttggatttg acggtgagta ccattcgcga tgcggctcaa
     1501 cgcggccatc gcttctcctt gagaacctgt acataggatc atcgtttcat tggcaggcaa
     1561 gcgattgatt tcgtgtgcat ctacaaatgt gccgtctgga actttgatat atcctaaacg
     1621 ttgaccgttg acgatggcat tttccatgct tcgtccgaaa acagcgattt tacgaccagt
     1681 tgccacagcg gcatctgctg cttgctgcaa acggaagatg ttagaagcaa agctggcaaa
     1741 aatgatacgt ccttcgattt ttgaaaagat cttcaaaata gaatgcccaa tcgttttttc
     1801 tgatttagta aatgttggaa tctcagcatt tgtactatca gataaaaggc acaagactcc
     1861 ttcttccccg atacgagcca ttttatgcaa gtttgctggt tcgccaacag gagtgaaatc
     1921 gaatttgaag tctccagtcg ctacgatatt tccagaaggt gttttcacca cgatccctaa
     1981 tgcatctggg atactatggg tcgttctgaa aaagctgaca gaagtctttc taaaacggat
     2041 gatcgtatct tcattgatct catggagctc tgcatctcga agcaagccat gctcatctaa
     2101 tttgtttgtg atcaacgcta atgcgagagg accagcatag ataggaatat ttgcttgttt
     2161 cagcaagtag ggtattccgc cgatgtggtc ttcgtgcccg tgagtaatca ccaaggcttt
     2221 tactttgtgt aagttttgta tgatgtagct gtagtcggga atgacatagt caattccaag
     2281 taaatcgtct tccgggaatt tgatcccggc atcaatcaaa atgatttcat cttggaattg
     2341 taccccatac gtatttttcc cgatttcacc taatccacca atagcaaaga cgcctgtctc
     2401 gttatttttg atggaaagtt tcatattaaa actccactaa tgagaaatca gcattttctt
     2461 tttcgtactc tagatgactt ccttctagaa gttgaacgaa ttcaatattg tatggggtat
     2521 tttcttccac acgttggcga gcgtctacat cgctttttgc ctcaatgtaa agagattttg
     2581 tttcttcccg tttagggttg cgtacttttg tttcttgata ataaacttta tagatcattg
     2641 ttttgtctcc tttatctgcg taaagcagtg atttagctag ttgatttttt tagacaacac
     2701 aaaacgaggg gacaagccac ctggtttttc atatgctttg actagaatac tggattcgag
     2761 tcaaagagac aagcctcgtt tttgcttatc tgttcggata ttttttcaac cgtaccttct
     2821 gtataaaaaa tatacaacaa ttaattgtta ttctagcata aaacagcaaa taagtataga
     2881 aatcaactag attgaaagaa
//
LOCUS       DACDKL010000234         2821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDKL010000234 DACDKL010000000
VERSION     DACDKL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.238"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            274..2169
                     /gene="asnB"
                     /locus_tag="IV329_002564"
     CDS             274..2169
                     /gene="asnB"
                     /locus_tag="IV329_002564"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8941716.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(2364..2810)
                     /locus_tag="IV329_002565"
     CDS             complement(2364..2810)
                     /locus_tag="IV329_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8941717.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT          918 a          483 c          534 g          886 t
ORIGIN      
        1 acacgatctt taggtcgtgt ctttttttgc tggttttttt ggaaatagca tccactaata
       61 tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta aattagatat
      121 tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg tgacgaaatt
      181 gtaagagaga aaatattcgt ccgctattta ttctatgata agatgcatat gaatgaaata
      241 aacagtaatt ttatattagg atgtgaaagt taaatgtgcg gtattgtagg ttttgttaac
      301 gacaaggaca acaaaaaaac aattattaat acaatgatgg atcgaatcgt tcaccgtgga
      361 cctaatagtt caggagaata tatcgataaa catgttgctt taggattcag aagattaagt
      421 attattgact tagagggcgg tactcagcct atttacaatg aagatcggac aaagatcatt
      481 atttttaatg gcgaaatcta taattaccag cctttgagag aagaattgat tgcagctggc
      541 cacgtttttc aaacacatgc agatacagaa gtccttttgc atggttacga agaatgggga
      601 acagaattat tgcaaaagat ccgcggaatg tttgcttttg ctatttggga taatgaaaag
      661 aatgagttat tcggtgcaag agaccacttt gggatcaaac catattatta tgcagaaatg
      721 aacggaacat ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc tgattttaac
      781 aaagaattga acaaagaagc gttgaaacct tatatgactt tccaatattc tccattaaac
      841 ggcgaaacat tcttcaaaga tgtctatcgt ttgcctgaag gccattatta tacgtataaa
      901 gatggaaaaa tagacattca gcaatattgg gatgcagatt ttgaaacaaa agaaacgcat
      961 tcccgacaag aatggatcga aaaaatcgac gaaaccgttc aagcttctat tgaagcacat
     1021 acggtcagtg atgtagaagt tggttctttc ttatccagcg gggttgattc cagttatgta
     1081 acctctgtat tgaaaccaga tcattctttc tcaatcgggt ttgatgataa aacctataat
     1141 gaagcgattg aagccagaaa actaaccgaa ctattggact tagataatac agcagcagtg
     1201 atcgacggag acatgtcgtt caaagcattc cctttgatcc agtatcactt agatgaacct
     1261 gattcaaatc cttcttgcgt acctttgtac tttttagcaa atcttgcttc acaaagtgtg
     1321 cgggtagtcc aatctggaga aggggcggat gaactgtttg cgggatacca aacgtatgga
     1381 ttccatacga attcaaaatt catccgtgtg attgctcaag gattgaaaaa attaccaaaa
     1441 ggaacacgct ataatttagg acgtaaaatc ggaaaaatga aaaacttcca tggacggatc
     1501 catttatatg aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc tcgagtgttc
     1561 gaagaatcag aagcatcaga agtgttaaca ccaaaatatc aaacagcacc atctgtggat
     1621 gaaatcatga ctgttcatta cgagaaaaca gaaggaatca aagatgaagt caataagatg
     1681 caatatgtcg atcttcatca atggatgcca aaagatatct tgttaaaggc agataaactt
     1741 tctatggcaa gttctttgga agtccgagta cctttattgg atatcgaagt catgaaattg
     1801 gctcaacaaa taccaagtaa gtttttattg aatcaaaaca acacaaaaga tattttcaga
     1861 caagctgcga acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt aggattccct
     1921 gtgccaatca aagcttggtt gaaagaagaa cacggctacg aacaagtcaa agcattattc
     1981 gaagcagatt ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt gctagatgac
     2041 catcacgaag gacgtaaaga agagcaaaga aaaatctgga caatcttcag tttcttgaca
     2101 tggtataaag tctactttgt agatgaaacg attccgcaag cagaagcaat cgactatgtg
     2161 actgtttgat gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg tttaacactg
     2221 agtgataaga aacaatttca aaaaacagct cttcccgttt tatagatatt atgacttatg
     2281 gttcggagtt ggcagaggaa caccattgtt tcgaacataa gggggttgtg acacgatttt
     2341 cgtcacaacc cctttacata tgttcaataa tcgatttctt tttcttttgt cccatctttt
     2401 ttataaatga acaaagttga ttctttgttt tgtgctactt gctttccttt ttcgattgct
     2461 tctttttttg tatcaaaatg gttgcttgcc ttttttgccc ctttagattt cacgatccat
     2521 tgttcttcct catattctac gatcacatct gaatcaagca gttttccagc acgaggattc
     2581 gtatcgtgtt catccgtctt gcttggattt ttttcttttt cgaaagttcg tttttctgat
     2641 tcactagcat cttcatacca tttttttgct tgatcaatgg caattgggat cgcacggtta
     2701 tcagggtacc cttcatctaa taatgcattt gcgatgtcga ttgctttttt tcttgtcaat
     2761 ttatctaagt ttttcattga tgcaggaaag tccttcatgt tccatggcat attagtatcc
     2821 c
//
LOCUS       DACDKL010000235         2816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDKL010000235 DACDKL010000000
VERSION     DACDKL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.239"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..1321
                     /locus_tag="IV329_002566"
     CDS             23..1321
                     /locus_tag="IV329_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014524919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8941718.1"
                     /translation="MLYPVILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLE
                     AKIMENFLKENQFVHKDDVLVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISK
                     GKNLFSTDDSFGYSNQLKSMLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIES
                     SDSKLQEWQQVQVAWSNNQSLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASI
                     NEQIEQLKKEVEQYQSEKAKLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNE
                     KQEKNQSVIKSLDDQLSKGILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSK
                     MKFTALLPANESTYIKEGMKVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVK
                     GTLKTTQSFPFRYGLTGELSLIVGEKTYWQQIVDVFLNKK"
     gene            1354..2388
                     /gene="sppA"
                     /locus_tag="IV329_002567"
     CDS             1354..2388
                     /gene="sppA"
                     /locus_tag="IV329_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8941719.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            2390..>2816
                     /locus_tag="IV329_002568"
     CDS             2390..>2816
                     /locus_tag="IV329_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8941720.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFS"
BASE COUNT         1076 a          379 c          561 g          800 t
ORIGIN      
        1 acatcatagc ttctaccgtt gggtgttata ccctgtaatt ctttttttat gtattattgg
       61 attgttttta acctttgcta aaaaagaggt tgttattcgg acaacggctc aattgaaccc
      121 cgaaaaaata gagaagttac aagtgccatt agaagctaaa ataatggaaa acttcctaaa
      181 agaaaatcaa tttgttcata aggacgatgt gttggttaga ttggattgtt ctttaataga
      241 gaatgaaaag gctcagatag aacaagaaaa ccaacgtatt actcaacaaa taaagatggc
      301 tcagctattt attgaaagta taagtaaagg aaaaaatttg ttttcaacgg atgacagttt
      361 tggctacagt aatcaattaa agagcatgtt gtcagaaaaa gaatcactcc gctacgcttt
      421 gaagcaaagt gaattaaatg atcaaaagca attagaagta tacgaaaaga caaaaagaca
      481 actagaaaaa caaattgaga gttcagatag taaattacaa gaatggcaac aagtacaggt
      541 agcttggagt aataatcaat cattaaaaga tttttcaaaa gaaatgatgg caaactatga
      601 gaattggcaa gaacaactaa ataatgtttc tgatgatcaa aaaaatcaag tgaaactgac
      661 aatttcagca agcataaatg aacaaattga gcaactaaaa aaagaagtag aacagtatca
      721 gtcagaaaaa gctaaattag ttaaaccaac tacttctgag aatgacagaa ttagtcaaac
      781 ggaaaaagga aagcaagagc tagaacaagc aataacaact gtaaaacaaa cagttacaga
      841 gctaaatgaa aaacaagaga agaaccagtc agttatcaaa agtttggacg atcaactttc
      901 aaaaggaata ctaaaagctc ctgtatctgg aacggttcat ttaaatgaag aaacaaaggg
      961 acaagcagaa ctagccaaag gaacagtgct tgcagaaatt tatccgatcc acgaaaaatc
     1021 aaaaatgaaa tttacagcac ttttacctgc gaatgaaagt acctatatta aagaagggat
     1081 gaaggttcat tttaaattgg atcaaaaagg tggggctccg attacgatcg acggaacttt
     1141 agatgaaatt tcagaaaatt ctactgccac tgagaaaggt gttttttatg cagtgaaagg
     1201 aacgttaaaa acaactcaat cgtttccgtt tcgttatgga ttaactggag agttgtcttt
     1261 aattgtagga gaaaaaacat attggcaaca aattgtcgat gtgtttctaa ataaaaaata
     1321 attattttta ctaagaatat aggaggagtt tatatgaata aaagaagatg gattgctgtg
     1381 ggagtagcgt gcggattatt gttactatcc attatcgtcg cgctaattcc aggaaaagat
     1441 aaagaggaag catcaaatac aaccctaaca ggaataaata agatttttta cggatctaac
     1501 gaaatcacag aagaaacgtt agaagaaggc gcatcaaata aaaagattgt caaactatcc
     1561 gtcaatggag ttattgcaga tacaggtgaa agcaatcttt ttagtaggga acaatataac
     1621 catcagaatt ttttaacaca gctaaagaaa attcaagaag acaaagcggt taagggagtg
     1681 ttattagaag taaattcacc tgggggagga atatatgaaa gtgctgagat tgctaaagaa
     1741 atggcaaaaa ttaaaaagct agatattcct atctatactg catttaaaaa tacagcggca
     1801 agtggagggt actacatttc tgctggttca gataaaatat ttgctacgga agaaacaaca
     1861 actggatcaa ttggtgtcat tatttctggt ttaaattatt ctggcttatt agaaaaactt
     1921 ggcgtaactg atgcgacgta taaaagtggt gcattaaaag atatgatgtc acctcaacac
     1981 aaaccttcag aggaagagaa taaagttata caggaatttg tgatgagtgc ttatgatcgt
     2041 tttgtcaatg ttgtagcaaa aggaagaaat atggatacta atgcagtgaa agaacttgcg
     2101 gatggaagaa tttatgatgg taaccaagca gtagaaaatg ggttggtgga tcaaattggt
     2161 tactctgaag atgcattaga ttccctaaaa aaagagaaga aattaactga tgcgacggtt
     2221 atagaatata aaaatgacac aactggcttt gcttcttctt ggttaggtaa taagattgct
     2281 gaatggcaag ggctaaaggc aactacatca gatcgtgtaa tttcagtttt tgaaaaactt
     2341 ggattttctg atactccaaa accaatgtat tactatggag ggctataaaa tgaataagaa
     2401 gagtggcatt gaaactttgc ctaattatgt atttgcggga ttttggttac gattttttgc
     2461 ttttcttatt gatttaattt gtattagcat catcacccat gctactatag gtctctttta
     2521 caaaatcatg ggactggaat ctgacaaatc cattattact ttttatggta tagcgtcatt
     2581 agctatttat ttaacctatt ttgtactttt gactaagctg aattcaggac aaacaattgg
     2641 gaaaatgatt tttgggataa gagtagtgag ctttgaggaa aaagagctaa gttggcaaac
     2701 tatctttatt cgtgagtgta tatgtaggtt tatattaaaa aattttgtat ttcaaattgg
     2761 gtatttggtg gctgctttca cgcccaagaa acaacatatt ggggattatt tttcta
//
LOCUS       DACDKL010000236         2799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDKL010000236 DACDKL010000000
VERSION     DACDKL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.240"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1279
                     /locus_tag="IV329_002569"
     CDS             <1..1279
                     /locus_tag="IV329_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941721.1"
                     /translation="DIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISL
                     LVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYP
                     MLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILK
                     EMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLV
                     FMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSD
                     MGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPV
                     YAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYC
                     LVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1266..>2799
                     /locus_tag="IV329_002570"
     CDS             1266..>2799
                     /locus_tag="IV329_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941722.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTIKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADI"
BASE COUNT          956 a          422 c          483 g          938 t
ORIGIN      
        1 tgatatctat tatcgatccg caattgcaga agcaatcgct cttacgatta tcccagctgt
       61 tttatattat gcttatggat tgatattcaa tagagaaaaa aaagtcatcg ctttatctgt
      121 cagtataagt ctacttgtgt atactcatgt cttaactaca ctattgtgta gtgcattgat
      181 tgcttgtatg attatagttc gtttgttttt aagcaagcaa ataaaaaacg atataaaatt
      241 tgtggtcgtg aatttagtaa aggctgtttt tttgacttgt gcattaacta gctatttttg
      301 gtatcctatg ttgcagcaga tgaattatca agaaatacac agtcccttca aaacagattt
      361 gcagctagaa gcactttcta tttcagatag tatcattcaa gcgttaaata acgatattac
      421 aacatacact atggggttat taggattggt tgcgttgatt tttcctttat tcatactgaa
      481 agaaatggag aaaaaagaaa aaatcatcta tgttggcgta gtttctacat gggtatgcgt
      541 aactaattta tttccatggt ttttgtttca aaaaacaccg ataagcttac tccagtttcc
      601 ttggcgtatc ttaggtatcc aaatcttttt ttcttcaatg atcataacgc ttgtttttat
      661 gaaaaagact atgaataaaa aatattcatg gttaatcatt ggcgctactg tggtgtttct
      721 actcgtcacg tctatagcag caaaagaaaa ttacgcaaaa gttatccaat caaaacacgg
      781 tcatattgtt atcaatgaag aaacactccc acgttataca acatcagata tgggaggatt
      841 atacgattac gccccaacag aagcgataaa agaaaggcct cacttagaaa aacatgaagt
      901 aaaagtagga gaatcgtggg aaaaagggaa gtataaagct gcagataata acattactta
      961 cacagtggca agcaacaaaa agcaaaaagt gacagtacca gtatatgctt atttaggtac
     1021 acaggtaaaa gtaaacggta aagtcgtaaa tgactcttat aaaaaacatg gcttagtgaa
     1081 tgtggatgta aatgctggag aaaataagat agaaattaca acaaaatata ctccaactgc
     1141 tctcatggct ttatggatca gtattctgac ttattgttta gtggtgtatt caagttttaa
     1201 acgatttgca cctaataagt tgaaagattc tcgatatgtg actaaaaagg agataaacaa
     1261 gaagaatgaa aataaataaa aaagtagtaa tattgagtgg attaattata gttagtagta
     1321 tatattcttt catctttttc aaaaatacac agagtattta taccactgga gatctttctt
     1381 ttcacttatc tcgaatcaag gggttatcga ctatttttac cagtccgatt aattatgaga
     1441 cattcaatta tactggatat ggtgtaaatt atttttatcc ctttctgact ttttttccag
     1501 ctgtaatgtt atattggatg acaaaaaatt tgattgtaag ctatattatt tacgtttggt
     1561 tattgaactt gtgtacaaca ctcgtagctt atcattatgg cgagcggttt ttaaaacaaa
     1621 aaaaagcggc atttttattt agttgtctct atattttctc tgcttatcgg acagtagaca
     1681 tctactatcg ttccgcaatt gccgaagcta tcgcaataac acttgttatc cctgtattat
     1741 tttatgctta tcaaatcatc tctggaaaag aagaaaagta tccgtctgta aagctagcat
     1801 tgagtatgag tttgctagtg tacagccatg tactatcaac attaatgagt acagcattaa
     1861 ttatcatttt tatttttatt cgtttggttt ctaaaggttt caagaatgca gattttattg
     1921 ctatatttaa aaaattattt agtgcggcag gaatgacatt ggttctgacg agtgcttttt
     1981 ggtatccgat gtttgaacaa atgctctacc aaaaaatcaa caaacctagc gtgacaaatt
     2041 tatatgctca tgcatctaat gtgttcgata gtctgactga agctatgaac aacgacttaa
     2101 ctacttactc aatgggacta gttggtctgt tatctttatg tattcccctg attttattta
     2161 aaaaattaac aaacatagaa aaaaagattt attatggtac gtgtttgact tggctagcta
     2221 caactagttt ggtgccatgg tatcttctgc aaaatacgcc agccaaattg ttacaatttc
     2281 cttggcggat cttaagcttg caaataatat tctcttctct gatattaacg atgatatttt
     2341 tcaaaaatag aagatacaat aagacaagag aactatttta tttatttatg actgttttgt
     2401 tattaatcac tttaactgtt tctagtaaaa aaaatttcaa tcaaaaaata atttctcagc
     2461 cgggacatat tgttgtaaac aaagaaacag tagaggcgta taccattaaa ggaatggatt
     2521 attttttata tgattattct cctatcgctt cattggagaa taaggagcat ataataaaac
     2581 acgaagcaaa gattggcagt caatgggtaa accttcctta taaaacaact ggtagttcta
     2641 tcatatatag agtaactaat gagaagagcc aagtaatcac acttcctgta tatgcttatt
     2701 taggaacgaa tgtagaaata aatcatcaac ctgtaaagga gcaggtgaat gctaatggat
     2761 tggtacaaat aagcggagtc aaaggagttg ctgatatta
//
LOCUS       DACDKL010000237         2795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDKL010000237 DACDKL010000000
VERSION     DACDKL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.241"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(254..577)
                     /locus_tag="IV329_002571"
     CDS             complement(254..577)
                     /locus_tag="IV329_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941723.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            764..837
                     /locus_tag="IV329_002572"
     tRNA            764..837
                     /locus_tag="IV329_002572"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:798..800,aa:Ile,seq:cat)
     gene            complement(1222..1719)
                     /locus_tag="IV329_002573"
     CDS             complement(1222..1719)
                     /locus_tag="IV329_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8941724.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1759..2568)
                     /locus_tag="IV329_002574"
     CDS             complement(1759..2568)
                     /locus_tag="IV329_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8941725.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          916 a          491 c          475 g          913 t
ORIGIN      
        1 ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac
       61 ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata
      121 aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata
      181 gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct
      241 gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat
      301 ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg
      361 aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga
      421 gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa
      481 ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata
      541 aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa aagaataaca
      601 ttttttctaa aataagcaaa taaaaattaa agtaacaaaa tagttatgac acaagtaaaa
      661 gaaataagaa taacaatatt attttactta tattttgaaa cttgtgagag tttgtcattc
      721 gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt tagctcagtt
      781 ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca aggtacataa
      841 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
      901 ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag
      961 gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt
     1021 tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta
     1081 agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga
     1141 tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat
     1201 cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag
     1261 ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc
     1321 tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc
     1381 aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat
     1441 ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc
     1501 ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag
     1561 ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa
     1621 tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg
     1681 atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat
     1741 atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc
     1801 attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc
     1861 aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat
     1921 gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt
     1981 gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc
     2041 tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa
     2101 tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt
     2161 ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt
     2221 cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata
     2281 gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat
     2341 ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat
     2401 tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac
     2461 gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc
     2521 atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta
     2581 ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat
     2641 gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg
     2701 ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt
     2761 agcgcttacc gatgcgtttt gtgataaaaa aattt
//
LOCUS       DACDKL010000238         2752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDKL010000238 DACDKL010000000
VERSION     DACDKL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.242"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..443)
                     /locus_tag="IV329_002575"
     CDS             complement(<1..443)
                     /locus_tag="IV329_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8941726.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="IV329_002576"
     CDS             513..863
                     /locus_tag="IV329_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941727.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="IV329_002577"
     CDS             1277..2095
                     /locus_tag="IV329_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8941728.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="IV329_002578"
     CDS             complement(2168..2527)
                     /locus_tag="IV329_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8941729.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          878 a          421 c          517 g          936 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc ta
//
LOCUS       DACDKL010000239         2751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDKL010000239 DACDKL010000000
VERSION     DACDKL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.243"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..1687
                     /locus_tag="IV329_002579"
     CDS             56..1687
                     /locus_tag="IV329_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8941730.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1690..>2751
                     /gene="uxuA"
                     /locus_tag="IV329_002580"
     CDS             1690..>2751
                     /gene="uxuA"
                     /locus_tag="IV329_002580"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8941731.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALE"
BASE COUNT          950 a          385 c          581 g          835 t
ORIGIN      
        1 cgattttata ctacaattgt aacggaaact aacatatcag tatggagggg taaatatggt
       61 tttgattaaa gactggcaag attctatcga aggattgaac aaaatgcaag ttcaactacc
      121 gtattttaat gtcgaagcgg tcaaacaaga aggtcagaaa catccagtgt ggcttcattt
      181 tggtggagga aatctttatc gaggatttca tgcagaaatt gctcaatgct tagcagatca
      241 aaatgaatta gaatccggag tagttgtgtg tgagacattt gatgaagaag tagtagaaaa
      301 agcttatcat gactacaaca atgatatttt ggagattgtt atgggtgaag atggtgaact
      361 caaaaaaaga cttttagcag taacagctga tagtttatat tgcaatccaa agaatataac
      421 ggactatcaa agagtggtta aatattttga agatcctaca ttacagttta caacgtttac
      481 tattactgaa aaaggataca gtttaaaaga ttctaaagga caattttttt cggtagtgga
      541 agcagatata aaatctggac ctaccaatcc tcaacatacg atgagtattg tgacatcctt
      601 gttgtatagc aggtttaatg ctggagagtt accgattgcg atggtttcta ctgataattt
      661 ctcacagaat ggtcaacgtt ttcaagatag catttttatg attgcagaag cttggcaaaa
      721 aaaaggatac gtttctgaaa aatttattgc ttatttaaag gataaagaaa aagtgacttt
      781 tccttggagt atgattgata gaattacgcc aaatccttcg gaaagtgtca aaatggattt
      841 attgaaggcg ggtttcgaag acttagatat aattcatact gcgaaaaata ccaatatcgc
      901 tgcttttacc aatactgaaa aagtccatta tctagtcatt gaagatagtt ttccaaatgg
      961 tcgacctaaa ctggaagcgg ctggtgtgat tatgacagat agagaaacag ttgataaagc
     1021 agatatcatg aaggttacga catgtttaaa tccgctccat acggcattag caataacagg
     1081 ttgtttgttg gggtataaat caattcaggc agaaatgcaa gatgatgatt tggttggctt
     1141 aattaaaggg ataggttatg tagaaggctt accagtagtt gaaagtccag gcgttattga
     1201 tccaaaacaa tttattgatg aagtaatcaa taaacgtttg cctaatccct atattccaga
     1261 tacaccgcaa cgaattgcaa cagatacttc tcaaaaagta gcgattcgct atggtgaaac
     1321 aattaaaaag tatgtcgaaa aagacggaaa tgcagaaaat ctaacattta ttcctatcgc
     1381 tattgctgct tggttacgtt atttattggc agtcgatgat caaggaaaaa attttgtgcc
     1441 aagtcctgat cctttattgg tagagttgca ggaaaaattc tcagtattga aattaggtga
     1501 acaagatcct gcaaagattc attgtgcggt acaggaaatc ttagaaaata aggaaatatt
     1561 tggtttagat ttatatcaag ttgagatagg aaagaaaatt gaaaaaatct tttgcgaaat
     1621 gctagtaggt cctcaagcag ttcgtaaaac aatcaataaa tatataacag aagttggagg
     1681 aaagtaacta tggaaatgac ttttagatgg tatggggcaa atgaagaaaa aatctcacta
     1741 caagaaattc gtcagatccc agcaatgaaa ggaattgttg gaacattaat ggatatacca
     1801 gccggagagg tctggccgag agaacgaatt aaagctttaa aagatgaagt cgaagccaat
     1861 ggccttacct tgaaagttat tgagagtgtt aatgttcatg atgatatcaa aattggtttg
     1921 ccaacacgag atcaatacat cgagaattat aaacagacga ttcgcaattt ggcagaattt
     1981 gggattgagg ttatctgtta taattttatg ccagtctttg attgggtaaa atcagatttg
     2041 gattatcatt tagatgatgg gtcttctacg ttagcctata tttcaaaaaa tattcctact
     2101 gatccagagg aaattattag aaaagtagaa aattctacta ataattttga attgccgggt
     2161 tgggaacctc atcggttatc acaaatcaag caattattag aagcctataa agaagttgat
     2221 gaagcgaaat tgcgagagaa ctttgcatat tttctcaaag caattatccc gacatgtgaa
     2281 gaagttggga ttaaaatggc agttcatcca gatgatccac catattctat tttcggatta
     2341 cctcgtattg ttaaaaatcg tgacgattta gaatggttgt gtaatgtagt ggattctcct
     2401 tctaatggta ttacgttatg tactggaagt attgcagaac gaccagataa tgatgtctat
     2461 gaaattctat ctgaattttg tcaacgggat cggattcctt ttgcacatgt tcgtaatatc
     2521 aaatatttaa gtgataatgg actcgatttt tatgaagcgc cgcataaatc agaatatggt
     2581 tctctagata tgtataagat attaaaaaca atgtacgata atggatttaa tggctatatt
     2641 cgtccagatc atggtcggat gatttgggga gaaaccggta gacctggtta tgggttatat
     2701 gaccgagctc taggtgcagt ttatttaaat ggtatttggg aagcgcttga a
//
LOCUS       DACDKL010000240         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDKL010000240 DACDKL010000000
VERSION     DACDKL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.244"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            515..1954
                     /locus_tag="IV329_002581"
     CDS             515..1954
                     /locus_tag="IV329_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8941732.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2249..2467
                     /locus_tag="IV329_002582"
                     /pseudo
     CDS             2249..2467
                     /locus_tag="IV329_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          784 a          478 c          590 g          867 t
ORIGIN      
        1 ctagaaaaga tgctgaaaat aaattgtttc aacctttgat tgacaaatat cgaggtaatc
       61 attaattatt gtggataata attaacagtg atatataaac atctataata tattattcat
      121 actattgata tttagtataa tcctatggaa ttgaatatca tagcaatgta tttgaaaagc
      181 aaattggaaa acaaataggt gcaacaccat aaatatgaaa aaattgggaa aaagtagaat
      241 ggaaatttgc ttacgtctca cgagatagct cacactatta atctgaagaa caatgaagtc
      301 gacttttaga aattgctaga agaaagtaaa gcgaattatc actagttact atagagtaag
      361 ttcaaataaa gcatctccaa cttacatggt tacatgagga tatcaaatcg aaattactca
      421 agcttcctat ggctagagtc gcgagaagtt gttgggcagt agacataggt tatattgttc
      481 ttagctacac gatcattaga aaggaaatcc tattatgtat agttatcaag aagatgcaaa
      541 agtccaaaaa aatcgctggt ggattttgat tgctatatct atgtttacct tcatgtccac
      601 attggatggc agtattgtca atattgcact acctaccatc tcagatgata tgggcatacc
      661 aatgaatcaa gcggaatggg tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt
      721 gctgtttggt aaaattggcg atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct
      781 gatctttgtc ttgggatcgc tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt
      841 cgcacgggtg gtacaagcca ttggtgctag tatgactatg gctacaaatg ccggaatcat
      901 cacggaagtc tttcccatac atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt
      961 ttcattaggc tcgattgctg gaccaggtct tggcggcttg attttgtccc aattttcttg
     1021 ggcgtacatt ttttggatca atgtaccggt gggaatcgta acgatgttga ttgctgaaaa
     1081 attcttacca aaagatatca ccatgagcgg ccatacaatc gatggagggg gctttgctgc
     1141 cattgcggct acgattatca gcttttttgg agcaatcttt ttggggcaag aaagtggctt
     1201 tttccatcca gtcccccttg tactgttcgt aatttcagtg attgcctttg tcagcttttt
     1261 aaaagttgaa aagaaagcag cctctccatt gatttctttc ggtattttta agaacaagat
     1321 ttttaccatg agcttgatta cagccttgtt gattttttct tccaacttct ttgtcaatgt
     1381 agtgattcct ttttatctgc aaaatgctcg cgggttgagt cctagctatg ctgggctgtt
     1441 gatgatggtc tttcctttga tgatggtcgt cggggcgcct ttaagcggct acctgaccga
     1501 ccgggttggc ccggagattt tggtattagt aggcttgggc ttattgtcgg taacccaggt
     1561 catgtatatg tttttgtcag tggactctcc tttgtggcta tacattgtcg ctactgccat
     1621 catgggtgcc ggcaatgcct tgttccagtc ccccaataat acgatggtta tgagctcggt
     1681 cactatcgaa aacttgggac tggcaggctc gatgaattcc tttgcccgaa atttggggat
     1741 ggtcagcggg atttcgcttt ctacgacggt cttatatcaa gcgatgtctg ctaagatggg
     1801 ccagcaagta actaactata tcgcaaatcg gccggatgtc tttttatatg gaatgaagat
     1861 taccttttta ggcagctttg ttttgtgttt tgttgccttt ttcctgacta tctggcgtat
     1921 caaaaaacaa ggaggtattc agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg
     1981 aggcctgcgt atgggtgagt gggatttcca tggatgatgg aagatgaagt gaacttctaa
     2041 aagccgaaaa actcaagtat tatcttcaaa atactagtgc tcaatgagat gcttgatttg
     2101 atcacaagaa gactgcgaaa gccgctggca accgcaagct aggtatccca ccgatggact
     2161 acgttttaga ggctactggt tatgaatacg gagccaacgc tctcatctgc ataagaatac
     2221 atcacccaga ttatgtaaag attttcgata cttctgtcaa aaaatcatta agatagataa
     2281 atgatttccg tctagtcaaa ttggttcaga atgtagacac acagatggta agaagtcact
     2341 cacaattcga gaatggactt gtcctatttg ccgtatacat cataatcgaa acgtcaacga
     2401 tagtatcaat attttgacca agggctatga atacaagcat tgccttagaa aacatcaaga
     2461 accttaggaa caacggggat agcttggtta ataagagata ccgctgttta ggcttgcaag
     2521 tatactcttt tatcaagaat tccgtggctt tagtcatgag aggcttaata ctatgaaaat
     2581 tttctttagc atttttgatt taggttcctt ttctaattat aataagatga ccactacagt
     2641 aacctactga aatatataaa cagccactgt agtaccattt tcaaagtagt ctatgagagg
     2701 agaatcttca ttcttatga
//
LOCUS       DACDKL010000241         2714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDKL010000241 DACDKL010000000
VERSION     DACDKL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.245"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(66..1160)
                     /locus_tag="IV329_002583"
     CDS             complement(66..1160)
                     /locus_tag="IV329_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8941733.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1349..2110
                     /locus_tag="IV329_002584"
     CDS             1349..2110
                     /locus_tag="IV329_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8941734.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2225..2665)
                     /gene="fabZ"
                     /locus_tag="IV329_002585"
     CDS             complement(2225..2665)
                     /gene="fabZ"
                     /locus_tag="IV329_002585"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8941735.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          854 a          544 c          464 g          852 t
ORIGIN      
        1 aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct gcaataacta
       61 attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa aaagtcaagg
      121 aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc gtttttcata
      181 tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt gtcaatcagt
      241 acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc tttgtagtat
      301 acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata actatgaagg
      361 ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc tactttcaat
      421 gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc acttgcgtac
      481 gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt cacttgtgtc
      541 gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt aggccaaggg
      601 ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata ttttctgaat
      661 ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg aggataaaaa
      721 ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt gtcaacgtaa
      781 ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg cgtcattttt
      841 gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc catcttatgg
      901 aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg attgatatca
      961 accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa ttctgaaaga
     1021 actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt atacccatac
     1081 ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc tggataaatc
     1141 gagattccta attttcccat attaccattt cctcctgcat cttttcgttt ttgtatccac
     1201 ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt ttaaaataaa
     1261 tgataaattc caataattta agtaggtatt ttccctctat tttgttatca ttaaagataa
     1321 caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt taagtattag
     1381 tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga cttcttctag
     1441 ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg ccagtagcgt
     1501 cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa atgaagatct
     1561 ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg aacacgcacc
     1621 aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg gactgacttt
     1681 ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg atggcactgg
     1741 aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc tttataatat
     1801 aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca gccagttctt
     1861 tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact atcctcaagc
     1921 aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt atactgtctt
     1981 cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg aaacagatag
     2041 ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc aagaagcaaa
     2101 acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc taggattcag
     2161 gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat atcaggtttt
     2221 atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact tttttatcat
     2281 ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg atttcaaaat
     2341 gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta ttgatcccac
     2401 cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc gaacctgctt
     2461 gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga aagtgacctt
     2521 ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt cctggttcta
     2581 aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat tccattactt
     2641 cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat tgccattttc
     2701 attactttga ctat
//
LOCUS       DACDKL010000242         2702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDKL010000242 DACDKL010000000
VERSION     DACDKL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.246"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..723
                     /gene="erm(B)"
                     /locus_tag="IV329_002586"
     CDS             <1..723
                     /gene="erm(B)"
                     /locus_tag="IV329_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8941736.1"
                     /translation="KYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAK
                     ISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLST
                     QIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPK
                     PKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNL
                     STVTYEQVLSIFNSYLLFNGRK"
     gene            complement(986..1849)
                     /locus_tag="IV329_002587"
     CDS             complement(986..1849)
                     /locus_tag="IV329_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8941737.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(1851..2123)
                     /locus_tag="IV329_002588"
     CDS             complement(1851..2123)
                     /locus_tag="IV329_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8941738.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(2140..2355)
                     /locus_tag="IV329_002589"
     CDS             complement(2140..2355)
                     /locus_tag="IV329_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8941739.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2447..>2702)
                     /locus_tag="IV329_002590"
     CDS             complement(2447..>2702)
                     /locus_tag="IV329_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8941740.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT          798 a          485 c          425 g          994 t
ORIGIN      
        1 aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg
       61 aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg
      121 aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac
      181 ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta
      241 cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta
      301 agcacacaaa ttattaaaaa agtggttttt gaaagccgtg cgtctgacat ctatctgatt
      361 gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg
      421 cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa
      481 ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa
      541 tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg
      601 tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc
      661 gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa
      721 taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga
      781 taaattatta ggtatagggc acctctaata actaccaatt aattcacctc aaaatgaaat
      841 tagaaaaaaa cagcgaaatt tttaaatcta tttcttatcg atacaaattc ctcgtaggcg
      901 ctcgggaccc caacatgagg aaatgttttt ataaaaaaag agtagttacc gttttttggt
      961 aactactctt ttcctttttt aaggtttaaa tacctggaag tttaggtgtt ttgggtattg
     1021 gtggtgtagg tggctgcaag ctttccaatt tttgttgaat tgctttaaat tcaggtgtct
     1081 cttggtgttt atttagaacc attttttgct ctattcgttc taaagtcggt tgaatttctt
     1141 tccctgatac tttacgattc aattcttttt ctaaggtttc ttttggacta atagaaggtg
     1201 tttccaaact tgaatagagt tttactcctt ctctgttata tagccttata tcgctaaata
     1261 agcccgtttt atgaagggtt tctaaattgg tcggtaagtt tttgacaaca atatcatgcg
     1321 cttgttttgg tgttgccctg gctgtcattg gatcatctgc atacatggtt tcatatcgtt
     1381 caattgttcc taaatatgag ttgattttag gtactgccat gacatacatt tttgtttcat
     1441 aacctttggc ttgaagcatt gttgcggttt gaataggaac gtctgttgtt cgtcctgtac
     1501 cttcgatcac caaattatac ccttgatcgc tcaaacggct tatgatcgct tctgtcatgc
     1561 gattagaata aggggtaacg tgttttacta cgtctttttc ataaagtttc actagttcat
     1621 caaaattagg gtgctgttgt ttaaaggtat cattatcaat gacaataaca ttcccttgtg
     1681 tttcttcaaa aattgctgat cgcaaactgg ttttccctga ccctggttgc ccaccaagta
     1741 aaaaagcggt tggcgattca accgcttttt ttccttgaat caattcttct aaattatcat
     1801 ttaagcgatt ctcaaattgt ttgtcagtaa aattgactat atttgccata ttaagccact
     1861 ttctctttat tcaaaacttg ttttgagtaa cgattcattg accgccaata ctcatgaacg
     1921 gcttgtaatt cttctaaaga ataatcaaaa agatttacac gttttgcaag ctttaattgg
     1981 tttaataaat tgacttccaa taattgagag tcatttttat ttaattcatg gttcaaaaca
     2041 tagtttaata ctcgattata aacgcttgcc gataattcgt taatgatctc tatttcaaat
     2101 gttttttcat acgtaactgc catttcattt tcactccttt taaagtttgt caggtaaata
     2161 tttccgtata tattcttcta aggcttgatc cataacttct tttacgtttc cgccattctt
     2221 tgctgtttcg atttttatga tatggtgcaa gtcagcacga acacgaaccg tcttatctcc
     2281 cattatatct ttttttgcac tgattggtgt atcatttcgt tttgcttttt gtgcgcctaa
     2341 atttcccaca atcactcact tctttctatt tcttcttact cttattttat catcaacaat
     2401 cacaaatcac aagtgatttg tgattaatca cttgtgattt gtgattctat tctttttcgt
     2461 tttctaattg aataattcgc tcaagcattt caaaaaatac gttcttatac atggataaaa
     2521 cttttttgtc atagccttta tgctctgtaa tgccgttttt agaccaggta cttactttat
     2581 tacttcgctt gataatattt tggaaaacca agttatcttc tttatgttgc ttatacagtt
     2641 cttccaggtt tgattttatc gttgcgctgt ccgtatcaac taaataagga acaaaaccga
     2701 tc
//
LOCUS       DACDKL010000243         2698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDKL010000243 DACDKL010000000
VERSION     DACDKL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.247"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            127..2541
                     /locus_tag="IV329_002591"
     CDS             127..2541
                     /locus_tag="IV329_002591"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8941741.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT          925 a          482 c          587 g          704 t
ORIGIN      
        1 tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat
       61 tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga
      121 cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa
      181 acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg
      241 tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg
      301 gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg
      361 gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag
      421 tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg atttagttat
      481 gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc
      541 tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta
      601 cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt
      661 ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct
      721 gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa
      781 agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat
      841 aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt
      901 cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta
      961 gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa
     1021 gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc
     1081 ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt
     1141 ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca
     1201 gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac
     1261 tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta
     1321 gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc
     1381 cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca
     1441 gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc
     1501 ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta
     1561 acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg
     1621 tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg
     1681 aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt acttcattta
     1741 ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa
     1801 ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca
     1861 aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca
     1921 ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat
     1981 ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac
     2041 aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat
     2101 caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc
     2161 caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc
     2221 gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca
     2281 atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca
     2341 cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc
     2401 aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata
     2461 gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta
     2521 gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa
     2581 tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat
     2641 taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttt
//
LOCUS       DACDKL010000244         2676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDKL010000244 DACDKL010000000
VERSION     DACDKL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.248"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..1363
                     /gene="dnaA"
                     /locus_tag="IV329_002592"
     CDS             29..1363
                     /gene="dnaA"
                     /locus_tag="IV329_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8941742.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            1561..>2676
                     /gene="dnaN"
                     /locus_tag="IV329_002593"
     CDS             1561..>2676
                     /gene="dnaN"
                     /locus_tag="IV329_002593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8941743.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITP"
BASE COUNT          880 a          504 c          516 g          776 t
ORIGIN      
        1 cactttttaa tgaaggagga ggccttatat ggtatccctc gatgctttat ggaatgaatt
       61 aaaagcaaca taccaaaagg atttatcgcc tgctagctat aatacatgga tcgaaacagc
      121 gaaccctcga acccttgatc aaaaccagct cgttgttgag gttcctagca agattcataa
      181 agaatattgg gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata tgttatcagg
      241 aaatgagatt atccctcgct ttattactgg cgaagaagca gagcaagaag aagttataga
      301 agaaaaaaat ccaaaagttg tggcacctag tccgctaaaa aaagccatgc tgaaccctaa
      361 atataccttt gatacgtttg tcattggtaa aggaaaccag atggctcatg cagcagcgct
      421 cgttgtagca gaagatccag gttctattta taacccgctc ttcttttatg gaggcgtggg
      481 acttggtaag actcacttga tgcatgcaat tggtcatcaa atgctgcaaa gtcagcctaa
      541 tgccaaagta aaatatgtaa gcagtgaaac ttttgctaat gatttcatca attcgatcca
      601 aaacaaaaca gcagaagagt tccgtcaaga atatcgaaat gttgatttac tgctagtgga
      661 tgacattcaa ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc atactttcaa
      721 tgccttgtac aatgaaggga aacaaatcgt actaaccagt gatcggttgc caaacgagat
      781 tccgaaactg caagaacgcc ttgtttctcg ctttgcatgg gggctgtctg ttgatattac
      841 accgccggat cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg ctgaacgtct
      901 agagatccca gatgacacac taagctacat cgcaggacaa attgattcta acattcgtga
      961 attagaaggt gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg aagatatttc
     1021 gactagtcta gctgcagacg cactgaaaac gttaaaatca ggaaaaggac atcctcaatt
     1081 gtcaatcctg cagatccaag aagaagtggc aaagtattat cacatccaat tgaaagatct
     1141 caaaggaaag aaacgcgtca aatctattgt tgttcctaga caaatcgcta tgtatttggc
     1201 acgtgagctc acagataatt ctttaccgaa aatcggagct gaattcggtg gcaaggatca
     1261 tacaacggtt atccatgctc atgaaaaaat ccagcagctg atggattcta gtgtcagtat
     1321 gcaaaatgaa gtatcagaaa tcaaaaactt tttactaaat taatttgtcg attctatcaa
     1381 ctgaaaatag catgtggata aaagaaaaaa acgttcaaaa gttatacaca ctttttccac
     1441 agctgttttt tcgtttctct cttacgtttt ttgagttttc cacagaatca acatgcccta
     1501 ttacttttat tattcttttt aactaataat aaataaacaa acattcaaag gagatacatc
     1561 atgaaagtta ctttaaaccg agctagcttt atgcaggaat tgcaaactgt tcaacgagct
     1621 atttcaagca aaaccacgat ccctattttg acaggtgtaa aaatcacact gacacaagaa
     1681 ggtttgactt tgacggggag caacgctgat atatcaattg aaactttttt gtctgttgaa
     1741 aacgaaaaag caaatatgca aatcgaatct actggttcca ttgttttaca agcacgtttc
     1801 tttagcgaaa tcattcggag acttcctgaa gaaacattta ctttagaagt tttagaaaat
     1861 aaacaagtag cgatcacttc tggaaaagcg aattttatcg taaatggatt agatgcagat
     1921 aactatcctc atcttcctgt tgtcgaaagc cataaccaga tgaaattacc tgtacacgta
     1981 ttgactaaac taatcaacga aacagttttt gctgtctcac aacatgagag tcgtccaatc
     2041 ttgacaggtg tgcatttcat tttatctgat aattctttat tagctgtagc tactgattct
     2101 caccgtctaa gtcaacgcgt gattccagta gaacaagcgg ctgatcattt tgatattgtt
     2161 attcctggaa aaagtttgat cgaattatct cgctcattaa caaatgaaga agaaatcgtc
     2221 gaaatcagca ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta tttctattcc
     2281 cgtttgctag aaggaaacta tccagatacc aatcgtttga ttccttcaag ttttaataca
     2341 gaggttgaat tttctgttcc tagcttttta gcggcgatcg aacgggcttc tttactttct
     2401 catgaaggcc gtaacaacat cgttcgttta tctattcgtc cagatgcggt tgttttatac
     2461 ggaaattcgc cagaaatcgg gaaagtagaa gaaagcttga gttatacagc aagcagtggc
     2521 gatccattag atatttcttt caatccggat tatatgaaag cagcgttgcg tgcatttgga
     2581 gatatgagca tcaaagtgaa atttatctct gctattcgtc catttacatt ggaaccaacg
     2641 gaagatggcg ttcaatttat tcagctgatt actcct
//
LOCUS       DACDKL010000245         2673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDKL010000245 DACDKL010000000
VERSION     DACDKL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.249"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            345..1130
                     /locus_tag="IV329_002594"
     CDS             345..1130
                     /locus_tag="IV329_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8941744.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1143..1994
                     /locus_tag="IV329_002595"
     CDS             1143..1994
                     /locus_tag="IV329_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8941745.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2118..2570)
                     /locus_tag="IV329_002596"
     CDS             complement(2118..2570)
                     /locus_tag="IV329_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941746.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT          841 a          439 c          571 g          822 t
ORIGIN      
        1 tgcataaaat ctcttgttta tgtataacta gcaattacaa taaaatgggt gataaataag
       61 cccaggaaag gggctgtgac aaaagtcttg tcacagcctt tttttccaaa taaatggttt
      121 ttcagaagta gcttcttcgg aaataagccg aaatttcttg aagctgacca cttctgtcac
      181 aacctctttc tttatagaaa taaaaggaca ctagcaattc atgaaaacga ttgctagagc
      241 gtatcatttg ctttagacta tatatcttgg tgtacactca ctttaaggcg gaaataaaag
      301 ttatccgtaa taacaggttt aggtatgaaa aaggagtggg tactatgtac aaggaattag
      361 aaaacaaagt cgcagtagta actggtggct caaaggggat tggaacagcg attgctcaac
      421 gattcgggaa agaaaaaatg aatgtagttg tcaactataa ctcggataaa gaaggagcag
      481 aaaaagcagt caaagcaatc gaagaggcag gcggaaaagc agtctctgta caagcagatg
      541 tcagttctga agaaggaata cagcgtttac ttgatacggc tttagaaaat tttgggacac
      601 tggacgtttg ggttaataat gctgggatgg aaaatcagca tccaacccat gaacttagcc
      661 ttgaagattg ggaaagagtg ttaaaagtca atttgactgg cgtttttatc ggaacgaaag
      721 cggctttaaa ttactttaca gagaataata aaaaaggaaa tatcatcaat atgtcttctg
      781 ttcatgaaca gatcccatgg ccaacgtttg ctcattacgc tgcttcaaaa ggcggggtaa
      841 aattatttac cgaaacggta gcaatggagt atgcaccgaa gggaatcaga atcaatagta
      901 taggccctgg tgctatccgt acacctatta atgcggcaaa atttgatgat cctgaacaaa
      961 aagaactctt ggaaagtatg atcccactag gacgcgtagg tgaaccaaaa gaaatagcgg
     1021 cgacagctgc atggttggct tcagacgaat ccagttatgt tacaggtatt acactatttg
     1081 cagatggtgg gatgacttta tatccttctt tccaaggtgg aaaaggataa gggtgaataa
     1141 aaatgggttt attgattgca ttgatcccgg cagttggctg ggggatccag ccactgattt
     1201 tgaaaaagat tggtggcaga ccaacaaatg agatattagg aactggtatt ggagcggtgc
     1261 ttgtcggctt actcgttcaa ttattcgctt ctccagctgg tatttcagtg actacatttt
     1321 tgattagttt actttctggt gcattttggg ttattggaca aacaggacag tatacatctt
     1381 tcaacttgat tggtgtttct aaaacgatgc caatttcgac tgcattacag ttagtcggaa
     1441 cctcgttgat tggtgtcttc gcttttggag aatggtctgg aagtatgggg aaaatcattg
     1501 gagcagcagc aatcattctt cttgtatttg gttctgcttt aacggcaatc agtgatgatc
     1561 ctggaaaaaa aggcggtatc acaaaaggaa ttagtctatt agcatcaaca agtattggtt
     1621 attgggtata cagtgcattg cctaaactag ttgatgcaag cggactttcc attttctttc
     1681 cgcaaatgat cggtgtcttt ttaggggcag tgatttatgt gattttccgt aaaccaaccg
     1741 cattttctga agacaagagt tggaaagcaa cgatcgttgg agccatcttt agtgtgtcag
     1801 cattggctta tattttttca gcaaatgaaa atggggttgc tactgcatat atcattacac
     1861 agctaaacgt agttatctct acattaggcg gtatgtacat cctgcatgaa aataagacac
     1921 caaaagaaaa aaaattaacg attatcggat tattgctgat cgtagtagga agcatggtca
     1981 cggtctttat ttgaggataa agatggtaaa atttcattta ttaaaaaaat atttgtttga
     2041 accaaacagt aagtacttat aaaaaaatag cgctagaatt tccaacatct ctggaatttc
     2101 tagcgctttt taattgttta agatacaagg gaaatatctg tctggatcaa taatttttca
     2161 ttgatccatt gactaaagtt tacgacataa ttgaagttac caccgattag tatctccgca
     2221 tgattgtctt tataaagatc gtattgtaaa agacggtctt ttttatttgc gtaaacatcg
     2281 ttatggtact ggactaattg ttctcggaat tgattgattg ctgtaaaatc acgatattga
     2341 aggttgaaac gcatcgtgat tggtgcattt agcatgaatc gctgcatttc ttttgctgct
     2401 tcctctgctt gatctccgta taggaactca aaaagattac gttcagaaaa tttccattgc
     2461 ccgccaacct ttgttccttt tagttttcct tcatgaagat aatttctgat agaacgtgta
     2521 gtaacaccta attgttcggc taattcattt actgagtaaa aatctttcaa tgttcaccct
     2581 cctatgtgaa tggttctact gacttagttg atatttgctt aatcacgaaa tgtagaaaga
     2641 acgattttaa ttaaggtcat tgtaaaaatc aag
//
LOCUS       DACDKL010000246         2671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDKL010000246 DACDKL010000000
VERSION     DACDKL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.250"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IV329_002597"
     CDS             174..518
                     /locus_tag="IV329_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8941747.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IV329_002598"
     CDS             519..938
                     /locus_tag="IV329_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8941748.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IV329_002599"
     CDS             complement(1030..1380)
                     /locus_tag="IV329_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941749.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IV329_002600"
     CDS             complement(1346..2566)
                     /locus_tag="IV329_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8941750.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT          994 a          480 c          523 g          674 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt t
//
LOCUS       DACDKL010000247         2601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDKL010000247 DACDKL010000000
VERSION     DACDKL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.251"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IV329_002601"
                     /pseudo
     CDS             75..470
                     /locus_tag="IV329_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IV329_002602"
     CDS             790..1104
                     /locus_tag="IV329_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941751.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IV329_002603"
     CDS             1208..2386
                     /locus_tag="IV329_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8941752.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT          849 a          382 c          531 g          839 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa c
//
LOCUS       DACDKL010000248         2563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDKL010000248 DACDKL010000000
VERSION     DACDKL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.252"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1767)
                     /locus_tag="IV329_002604"
     CDS             complement(<1..1767)
                     /locus_tag="IV329_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8941753.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQS"
     gene            complement(1721..>2563)
                     /locus_tag="IV329_002605"
     CDS             complement(1721..>2563)
                     /locus_tag="IV329_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8941754.1"
                     /translation="RYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFENVSFSYKEE
                     CVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDT
                     IINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTL
                     IGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKT
                     VIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDN
                     EKFL"
BASE COUNT          801 a          491 c          370 g          901 t
ORIGIN      
        1 tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct ttatgagatt
       61 tttgtatatt tctgaccttc gatacaactc attatgagta ccttgatcaa ctagttttcc
      121 ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat gcgatataat
      181 tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt tttcattttc
      241 cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt tcaaaaaagc
      301 ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt tttctccgac
      361 caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag ctaaacgagc
      421 tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat tttccaaaat
      481 acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa acaaagaagc
      541 tggagataca cgtttaatat catacccatc aattaaaata cagccttcat cataatcata
      601 cagtctagac actaatttca agatactagt tttcccggaa ccactagcac ctactaacgc
      661 agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg tattgttatc
      721 atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac tatctgatcc
      781 ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag ctaaataaaa
      841 tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta ttgcggctag
      901 taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt gaacacctac
      961 tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca tgactgaaaa
     1021 agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt tgaaaagtct
     1081 gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt ctatagtttc
     1141 ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg ctttcgtttg
     1201 ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa taacagctaa
     1261 tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa acagagccat
     1321 accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa cgtcagacat
     1381 gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg gtaaatttga
     1441 caaatcgtct gcaatttgca ctcttaaatg cgcactttct ttataagtac tattatataa
     1501 gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacaa acgatacggc
     1561 aaaataaagc caacggggct ttgtattttc taaaactaat tcgtctatca gcatcattat
     1621 aatgaatgct ggtaccatat taatgcagta tacaaaaaaa ctagaaatag aagctttagt
     1681 taaagcttct gcgccctgat cacttaaagc aaatttgttt ttataaaaac ttttcattgt
     1741 caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac acttgatgtt
     1801 tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac aagacaagaa
     1861 tttcatctac agatttaatg ctggataatc gatgagctat cataataact gttttttctt
     1921 tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca atagatgcac
     1981 tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca atagctatac
     2041 gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta tgctctcttt
     2101 cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca aatactttac
     2161 ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga ttgaatagtt
     2221 tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa tagtgattta
     2281 aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct gaaattagtt
     2341 tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt cttttttgtt
     2401 ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag ctgacatttt
     2461 caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt tggtcttgct
     2521 tcatttctaa ataaagggag tctagtgttt cgactgcata tct
//
LOCUS       DACDKL010000249         2549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDKL010000249 DACDKL010000000
VERSION     DACDKL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.253"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(611..886)
                     /locus_tag="IV329_002606"
     CDS             complement(611..886)
                     /locus_tag="IV329_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8941755.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            993..1757
                     /locus_tag="IV329_002607"
     CDS             993..1757
                     /locus_tag="IV329_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8941756.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1880..2383
                     /locus_tag="IV329_002608"
     CDS             1880..2383
                     /locus_tag="IV329_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8941757.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT          824 a          459 c          529 g          737 t
ORIGIN      
        1 tcgtagcgaa aaattttgaa aaaagacaaa aaatcaagaa agaaatactt agaaagaaaa
       61 gagtgattcc aagtgtttct ttcttattta aaaaactaaa aacttttttg ttctgtctgg
      121 aaaatatggg ttaagcaaat ttattgcaag ttatagatca gcttttttac tacctgtctt
      181 taatacttga ggcaaagtgc ctcaaaaaca accttatcga cgatgttcaa aagtcagttt
      241 cttggtatta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aattttcctt
      301 aatactcaaa actaagcgac tttttcacaa ccagtcctta atgcttgatg cagatcagct
      361 ttttcgcaac cttatcgacg atgttcaaaa gcttgcatct acggcattaa gtccagcctg
      421 ttgaaaaata tggagagcta tttttcacaa ccagtcctta atgcttgatg cagaccagct
      481 ttttcacaac cttattgacg gtgttttatc agctgcctct cggtattaag tcattacatt
      541 tcaaaatatg agaagctatt tggaaaatgt ttctccttaa tactcgaggc aaagcgcttc
      601 aaaaacaacc ttatcccacc acatcaaatt tctttcgttc ctcaatcatc ggatcttctt
      661 ggacatccac atactgcact cgtccacttg ggctcggaga cttcttgact tcttcgataa
      721 atttttggat gatgtcgtca tttccgattg cttcgattgt gactgagccg tcgtcttcgt
      781 tttttaccgt tccggtcacg cctaactgat cagcaaccat cttggtcata tagcgaaatc
      841 ccacgccctg gacacgtcct tggacattca ttttaacttt gcgcatgttc gtcacctttc
      901 tttcctgttt tataaatcaa tgataccgat ttttttagga tgaaacaagc tcaagcacct
      961 attccatgat ataatcagtt gaggtgagga atgtgaaaga gatccaatca gtaaaaaatc
     1021 caagaatcaa agaattaaaa aaactacata aaaagaaata cagagaagaa aaggcagaat
     1081 atcttttgga agggttccat ctgatcgaag aagctgcgtc gtctgatgca gaaataaaag
     1141 aactttttgt gacacagcga ggtatgaaag aatggggaga ctggctggct gtaaacaaag
     1201 agatttctac ttttctagtt tcagatgagg tcatgaccgc tatgtcagat ctacctacac
     1261 cgcaagggat cttagcagtg atggcgttaa cggctgaagg cacgccagat ttttcaggag
     1321 gatggctgtt attagataat gtacaagatc ctggaaatgt agggacgatg atccgtacgg
     1381 cagatgcatt tgatatggct ggtgttttat taggcaaagg aacggcagat atttatagta
     1441 caaaggtgct gcgcagcatg cagggaagca attatcattt gcctgtgttg caaaaagatt
     1501 tgacagaagt gattccttta atgaaagaca aagggtttac agtgtatggc acagaattaa
     1561 atgaagaagc acacgcctta gacaaagtag aaaaatcaga taacttcgca ataatcatgg
     1621 ggaacgaagg tcagggggta agtcaggaaa tcttgtcttt gactgatcag aacgtatata
     1681 tcccgatgaa aggaaatgcg gaatcgttga atgttggggt tgcggcaggg attgttcttt
     1741 atcatttttc tttatgatta aggaactatt aagctttggc tattttatcc aaggcgacta
     1801 gaataatccg aacactggtg gtatacttag atcaatttca gaagaaaatg aaattcaaaa
     1861 aggtggcaga tcgataatta tgacagaaaa atggcgtgaa gacgaagaat atatgtctta
     1921 tgtagaagat cttttagaaa cagaagcagt gaaaaaatta gcgaattaca cacagcatgt
     1981 tcattccact cgtttggagc attccatcag tgtttcttac tatagttatc tgctcgcaaa
     2041 aaaatggggt ggcaatgcta aagcaacagc gcgtgctgga cttttgcatg atctgttcta
     2101 ctatgattgg cgtacgacga aatttgatga aggcacacat gcgtatatcc atccaagaat
     2161 cgctgtaaag aatgcagaaa agatcaccga tttgtcggat cttgagcgag atatcatttt
     2221 gaagcacatg tggggcgcaa cgattgcacc gcctaagtac aaagaaggat atatcgtaac
     2281 attcgtagac aaatactgcg cagtcaaaga agccgctcag cctatgtcag caagtatgcg
     2341 taagagatgg caacgttact tcggaaaaga atcctcgatt taacaggtaa aaccaataat
     2401 agaccatatt gaggttgtga cagaagtggg tcagcttcaa gaaataagaa ggaatttacg
     2461 aaaattgctt ctcaaatttt tgtgaaattc cggcttattt tttgaagaag ctacttctgg
     2521 aacaccgttt gtttgtagaa agggacttt
//
LOCUS       DACDKL010000250         2531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDKL010000250 DACDKL010000000
VERSION     DACDKL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.254"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1012
                     /locus_tag="IV329_002609"
     CDS             5..1012
                     /locus_tag="IV329_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8941758.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1024..2028
                     /gene="neuB"
                     /locus_tag="IV329_002610"
     CDS             1024..2028
                     /gene="neuB"
                     /locus_tag="IV329_002610"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8941759.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2025..>2531
                     /locus_tag="IV329_002611"
     CDS             2025..>2531
                     /locus_tag="IV329_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8941760.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVF"
BASE COUNT          927 a          324 c          506 g          774 t
ORIGIN      
        1 ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga
       61 aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga
      121 agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat
      181 tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt
      241 tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa
      301 aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca
      361 ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa
      421 gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat
      481 attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt
      541 tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt
      601 tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt
      661 tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt
      721 aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg
      781 tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc
      841 atatgaaaat gataaaatgt atatcattcc accacactat catgctactg acgaactgta
      901 caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag
      961 tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt
     1021 atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta
     1081 gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt
     1141 caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag
     1201 gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa
     1261 tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact
     1321 ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt
     1381 ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca
     1441 gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc
     1501 atagatagtc cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat
     1561 attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc
     1621 gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt
     1681 gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat
     1741 cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat
     1801 ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta
     1861 gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa
     1921 cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa
     1981 agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta
     2041 tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac
     2101 aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat
     2161 ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt
     2221 ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata aatggtttca
     2281 caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa
     2341 tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag
     2401 gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg
     2461 ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt
     2521 ggagagtttt t
//
LOCUS       DACDKL010000251         2521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDKL010000251 DACDKL010000000
VERSION     DACDKL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.255"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..369)
                     /locus_tag="IV329_002612"
     CDS             complement(<1..369)
                     /locus_tag="IV329_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8941761.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDS"
     gene            588..1958
                     /locus_tag="IV329_002613"
     CDS             588..1958
                     /locus_tag="IV329_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP8941762.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1979..2263
                     /locus_tag="IV329_002614"
     CDS             1979..2263
                     /locus_tag="IV329_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941763.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          854 a          379 c          519 g          769 t
ORIGIN      
        1 actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa agtatcgaat
       61 aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc tactaggaaa
      121 gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt ggctagtata
      181 gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt gcttcagttg
      241 atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata tgttagaaac
      301 agttctcata atttccttga atgttataaa agtatctgta taatgttcag tatattttga
      361 tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg cgttttttgt
      421 tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc accacgcgta
      481 ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa agtatcaatt
      541 ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg atggataaat
      601 ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa gaagtctttg
      661 ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata actggggagt
      721 attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa gagcgcgatg
      781 tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat tttgcgagac
      841 tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc caaagtatgg
      901 aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat catgaccaat
      961 ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat cagcttagga
     1021 gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca tatttgaaaa
     1081 gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt gtaatttatg
     1141 ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag gcattgaaat
     1201 ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta cttgaaaaaa
     1261 tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt gaacacatca
     1321 tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg gaagaataca
     1381 caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat aattcggccg
     1441 cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt catattcgta
     1501 tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct gccagaagtg
     1561 aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg ggcgactatg
     1621 aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca actgggtatt
     1681 tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct aagattaaag
     1741 atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat ggaatcattg
     1801 aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct gtttcgaatg
     1861 cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa tatgataacg
     1921 gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg gtgttaggtt
     1981 ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa agaatccttt
     2041 tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta aatttctttc
     2101 gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata tctgggggag
     2161 tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta aggcgtatgc
     2221 attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt ttttaaacgg
     2281 agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa agaaaaatga
     2341 caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc accgacaacg
     2401 tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat ttaaatgatt
     2461 tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt cgattttcac
     2521 a
//
LOCUS       DACDKL010000252         2501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDKL010000252 DACDKL010000000
VERSION     DACDKL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.256"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..1120
                     /gene="xerS"
                     /locus_tag="IV329_002615"
     CDS             35..1120
                     /gene="xerS"
                     /locus_tag="IV329_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8941764.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(1404..2188)
                     /locus_tag="IV329_002616"
                     /pseudo
     CDS             complement(1404..2188)
                     /locus_tag="IV329_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2223..2372)
                     /locus_tag="IV329_002617"
     CDS             complement(2223..2372)
                     /locus_tag="IV329_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8941765.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(2365..>2501)
                     /locus_tag="IV329_002618"
     CDS             complement(2365..>2501)
                     /locus_tag="IV329_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8941766.1"
                     /translation="AKAIMVSKSYTYYENRRLGLVPVESVEEVLGMKIEVEEDFQNHG
                     "
BASE COUNT          856 a          442 c          360 g          843 t
ORIGIN      
        1 ctcttctccc ctacttattg aaaggatgaa tgacatgaaa aatacagaaa tatatcaaaa
       61 aatagataca atcatacttc atatgcccga ttatataaaa gattttgttc aagatcgtga
      121 ggataaagat caatcacctc gaaccacttt agaatatcta aaaaattata agctctttta
      181 tgaatggctt ctgagtgagt ctatcgttcc tgatacttct atcacctcga taagacagtt
      241 aactgcctat gacctaaact tgtacaaatc tcatttgaaa cgtcgcacaa aagaaaatgt
      301 aaaaaaagag acaacaaaaa ctaagctcaa agataataat aacttaggtc tatctacttc
      361 tactatcaat agaaatatca cggcattaaa ggttctattt aaatatcttt cgaaaagttc
      421 caataatcct ctaggaaaac catacttaga caataatcct atggatcaag tagcaaccat
      481 cacagataaa actacgttag ctgcacgtgc caatgccatt gagaaaaaat tatttctaga
      541 taaggataca caaaattatc tagattatat tgccaatgaa tataaaaata ctttgagtaa
      601 acgagctttg atctattatc aacgagatgt agaaagagac ctggcgatca atgcactgat
      661 tttaggatct ggattgagac tctccgaggt tataaatatt aatttagatg acctatcttt
      721 agataagaat aatgtggtag taactagaaa aggaaataaa agagatgctg tcaatatagc
      781 agcattcgca atggaatatt tagctaatta tctagctatt cggaaagaac gatataaagt
      841 aacagaaaac gaaaaagctt tatttctagc aatttaccaa ggagaagcca agcgtatatc
      901 cggtattgca atcgaacgga tggtagcaaa atattcaaaa ggctttagaa ttcaagtcag
      961 ccctcacaaa ctgcgtcata cattagctac tagactctac caacagacaa attcattggt
     1021 attaactgcc caacaattgg gacattcaag taccaataca acaactttat atacccatat
     1081 agacaatgcg gctacgatcg atgcattaaa tgctttatga gtataatgta taaataggcg
     1141 gaaataagaa acaacacgct agaaaagcct gtattatagt ttttctagtg tgtttctttt
     1201 ggtgtgatcc tacttttgtt tatcatttat tttttattat cgtaaatagg tgtcggtttg
     1261 attttagaaa aattagtttg gatataatct gtgggatcat ttgttatctc tctcaatgaa
     1321 tttagataca aatgaccata agttcctaaa gtcgtttgta catcagcatg ttttaacctt
     1381 tctttaattc gaagtgcatc tttttattgt gatataagca aagaagcatg actatgcctt
     1441 aatccgtgta tgcggattat atgaacacca acgatcgttg catgtcttct cataatatga
     1501 cggattgtat atttctgagt aggtgttcca ttataagaaa gtacaaacga catgcctcct
     1561 attgaaactt gacgctcctt ccaagctttt aaaaaggtaa ttgtattgtc gtctagttcg
     1621 attgtacgaa tactagaagc cgtttcgggt tctacaaact cataggtttt ggctctctta
     1681 taatagagtg ttttattcac acgcaagttt ttattttcaa aatcaatatc tttccaagtt
     1741 aatgcagtgg cttctcccaa acgcataacg gtcataaaaa gtaaccacat acaaataaaa
     1801 ccaaaatgtt ggtacaaatc atcaatataa aaagttgaaa taacttttgt aaattcttct
     1861 tttgtccaaa aatcaactgt agttttcctt ttcttcacat ttccaataac ttttgcagga
     1921 tttttttcaa gtagtcccaa aacaactgct cgatctaatg acatctgaaa aatcccatac
     1981 atgtttctta cataagaatt tttatatttg ctcaataaat tattttgcca cttttgaata
     2041 tcaattggac gtatttctct taactccatt ttaaagaaat aagaaaagtt tttttgattg
     2101 tagataaccg atttttatat gttctttctt ctactttccc ttgataataa ggtaggaata
     2161 atttataaaa aaactcctca aacaacatag tactttgaga agtttctgct tcttttccat
     2221 aattagaaac aagttcggta tacgcttttt ttcttcttta actgttttaa cccctctacg
     2281 tttttttgta ctcttttccc tgttcgtttg tcgaatccca aactagcagt aaaatgatat
     2341 ttcccatttt tatctttttt aaaattaacc atgattctga aaatcctcct ctacctctat
     2401 cttcatacct aaaacttctt ctacagattc aacaggtact agtcctaatc ttctattttc
     2461 gtaataggta tagctttttg aaaccataat tgcttttgcc t
//
LOCUS       DACDKL010000253         2500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDKL010000253 DACDKL010000000
VERSION     DACDKL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.257"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..726
                     /locus_tag="IV329_002619"
     CDS             100..726
                     /locus_tag="IV329_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8941767.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            749..1453
                     /locus_tag="IV329_002620"
     CDS             749..1453
                     /locus_tag="IV329_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8941768.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            1645..2478
                     /locus_tag="IV329_002621"
     CDS             1645..2478
                     /locus_tag="IV329_002621"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8941769.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT          741 a          443 c          521 g          795 t
ORIGIN      
        1 agataagtaa agagaaataa gtacctgttt ttgtttccta gcaatgaatg cgttatacta
       61 atggatgaaa gaagttttgg ctatcaagga ggaatgcaca tgcatatcga aagattaaaa
      121 acaggaatga ttgaagaaaa ctgttatttg gtctacaacg aagaagcact tttgatcatt
      181 gaccctggag aagatgcgga acgaatcaaa caagaaatca aaaaaacaaa ccaacagccg
      241 gtagcgattt tgcttaccca tactcactac gatcatatcg gtgcagtgga acctttacgc
      301 gcgtattatc aaatcccagt ttatgtcagc ccactagagc aaaaatggct aggtgatccg
      361 attttgaatc tttctggact cggtagacat gatgatattg ctgatgttat tgtctcgcct
      421 gcagaatatg aatttgaaat gaaaacttat actttaggaa acatgtcttt tcgcgtcgtc
      481 cctaccccag ggcattctat aggaagcgtc agctttattt ttgatgattt tgtcgtttct
      541 ggcgacgccc tgtttagagg aagtatcggt agaaccgatc tgtatacagg gaatttagaa
      601 caattacttc atagtattcg gacccagctg tttgtattac ctgatgaatt tgcagtctat
      661 ccaggacatg gcgatgcaac gacgattgaa caggaaaaaa ggacaaatcc ttttttcaat
      721 cgttaaaacg acttaggagg agaataaaat gacagaaact acactttata tcgtacgaca
      781 cggaaaaacg atgttcaata caattgaacg agtacaaggc tggtgtgata cacccctcac
      841 aaagcaagga caagaaggca ttcattattt aggtcgcggg ttaaaaaatg tcgacttttc
      901 atttgcttat tctagtgaca gtggtcgtgc aatcgaaact gcccgtatta ttctaggaga
      961 acattctaag ggaaaagaaa ttccttattt tattgaaaaa aggattagag aatggtgttt
     1021 cgggtctctt gaaggtggat atgacatgga aatgtggggt gtgatccctc gtgtattgaa
     1081 ttttaaaaca tatgatgaaa tgtttaccac agaagtttct tttgaacaaa ttgctaatgc
     1141 tgtatacaac ttagataccg ctgggtgggc ggaaacttat gaaacattgc gagaccgtgt
     1201 atggacagga tttgaagata tcgcccatca ctgcgaaaaa aatggtggtg gaaatgtttt
     1261 agtcgtatct catggtttga cgatcgcctt tttgcttaat ctgatcgatc ctaaacagcc
     1321 tgttcgtgct gggcttttaa atggcagcgt aacaaaggtc acttatgcaa acggtcgatt
     1381 ttctatccac ggcatcaatg atacgtctta tatcgataac gggaaaacac atagtcgcga
     1441 ctcgtttcta taatggggat attttaattt ttaccagaga atcataaaag gcaaagtctg
     1501 aacgagggct ttgtcttctt ttttctcttc gtgtacttat ctgctttatt tctcaagttt
     1561 ttcttaatat taaaaaacag attgggtttc tcaaaaaata acggtatact ataagatgta
     1621 tcttaattgg gaaggaagga atttatgttt tttgcaaata ttatcaaagc agtgattctc
     1681 ggtattattg aaggaataac ggaatggctg cccatcagta gtactggtca tttgatccta
     1741 gcagatgaat tcatcaaact ggatgccagt tcacaattta tgtcgatgtt caatgtcgtt
     1801 atccaattag gggcgatctt ggctgtcgtc gttctttatt ttcacaagtt aaacccattt
     1861 tctccaagta aatcaacggt agaaaaaaaa gatacgtgga cactttggtt taaagtgatc
     1921 gttgcttgca taccagccat catcattggt ttgcctttgg atgattggtt agaagcaaat
     1981 ttccacaagt tcttaccagt tgccattgtt ttgatcgttt acggtattgc ctttattatt
     2041 gtcgaaaaac gcaacaaaaa caaaacacct cgttggactt ctctgaatga ctttacgtac
     2101 caagcagctc ttcttgttgg gttattccaa gtcttggcac ttgttcccgg aacatcccgt
     2161 tctggcgcaa cgatcctagg atctatcttg attggtgctt cgcgttttgt cgctacagag
     2221 ttttcatttt tcttagggat tcctgtcatg tttggcgcaa gcggcttgaa gatcgtcaaa
     2281 tatttagctg aagggaattc attcaaaatg gaagaaactg ttgttcttct tgtaggaaca
     2341 ttcgtatctt ttgtggtttc cgtcttagct atcaaattct tactaagcta tttgaagaaa
     2401 aacgacttta ccgcttttgg ctggtatcgt attatccttg gtatcatttt gattggttat
     2461 tggtttattg caatgtagaa aaaaccagaa cacttctctt
//
LOCUS       DACDKL010000254         2461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDKL010000254 DACDKL010000000
VERSION     DACDKL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.258"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..909
                     /locus_tag="IV329_002622"
     CDS             13..909
                     /locus_tag="IV329_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8941770.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            912..1625
                     /locus_tag="IV329_002623"
     CDS             912..1625
                     /locus_tag="IV329_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8941771.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            1645..2163
                     /gene="msrA"
                     /locus_tag="IV329_002624"
     CDS             1645..2163
                     /gene="msrA"
                     /locus_tag="IV329_002624"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8941772.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            2160..2387
                     /locus_tag="IV329_002625"
     CDS             2160..2387
                     /locus_tag="IV329_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8941773.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT          900 a          436 c          471 g          654 t
ORIGIN      
        1 agaggtgaaa aaatgaaatt aacgaaacga catttgatcc tcgttttgtt cccattatta
       61 attgcgatca tcatctatgc aggtttagct gcttctatac cgaaagctgg cccactaatg
      121 aaaccagtaa ctactgtgag agaagatcca aataaaaaag aaaaacttca ctacatagct
      181 atcggagatt cgctgactga aggagtagga gatcaaacca agcaaggagg attcgttccc
      241 cttgtcgcaa atgatctaaa agaccgttat aatttgaccg cggttgaaac agaaaactat
      301 ggtgtaaatg gtgaacgaag tgaccagatc ttaaagcgag taaaaacaaa agaagaaatt
      361 cgaaacaata tcaaaacagc tgattttatc acactgactg ttggcggtaa tgatttgatg
      421 aaagttatcc aaaataattt attcgggtta acggtcaatt cctttaaaaa gccactgaaa
      481 aactatcagg aaaatgtcac agaactattg gaagaatttc gatccctcaa taaaaccgca
      541 cctatttaca ttttaggcat ctacaatcca ttttatttga actttcctga gatcactgac
      601 atgcagacaa ttgttaacaa ttggaacaaa ggaacagaaa cggtgacaaa aaaaatgccg
      661 aactgctact ttatcccaat caatgatttg ctttatcaag gattgaatca ggaagtaggt
      721 gtcacagaag aaggaacaga tcaaacaact agcacgacag aaaatctaaa tggcatcaaa
      781 aataatgtgc tctatgaaga agatcatttc catccaaata atttaggtta tcaattgatg
      841 gcaaatgcag tgagagatca gctgatcgag acacaaaacc aatggttaga gaaggaggat
      901 gatcaataaa catgaaaaca agatctgacg tatcttcaaa caaaacaaag aaaacaaatt
      961 actggaaatt cgccttcctt atccttcttg gggtagtact tggttcgacc gtatttttag
     1021 gcagccgtat ttttgctaat cgggaaccag aactcccaga aataccagct cttacggaaa
     1081 gacaaggaga tcctgtctta acgatcaatt caaataaaga aaaagtcaat caaatcatca
     1141 gctttttcct tagtgagtat caaaaagata gtgatattga atataaattt tatttggaaa
     1201 atgaagcact actcaatggt actttcgaag tactaggttt tcctatcgat ttctatttat
     1261 attttgatcc gtatgtgatg gaaaacggaa atgtccaact aaaagctaga agtctttcta
     1321 tcggaacatt gagtttgcca atcaaagatg tgatgaatat gatcaagaga aactataaat
     1381 taccagattg gatcgaagtc aatacagatg atctgactat catgcttagg ttggatcaat
     1441 tccgaatgca aaacgggatg tatattaaag cagataaaat cgacttagta aatgatgaca
     1501 ttcggtttag tctatatttg ccaaaagata cctctgatgc ttcaaacaac tcaaatacca
     1561 acaaaactag caccagcagt actgaaagca agacaaaaga aaataaaaaa tcatcggatg
     1621 agtagtatta gagggggaat cataatggaa cgagcaatat ttgcaggagg ctgcttctgg
     1681 tgtatgatcc agcctttcga cacgcttcct ggtatccata cagtgatgtc aggatatact
     1741 ggagggcatg taccagatcc gacttatgaa caagtaaaaa gccaaacgac tggacataca
     1801 gaagcagtgg agattatcta tgatccaaca ttgatttcat atgaacaatt agtagaactt
     1861 tactggcagc aaactgatcc aactgacgct ttcggacaat tcgaggatcg cggtgacaat
     1921 tatcgcccgg tgatctttta tttgacagaa gaacagcgga tcattgctga aaaaagtaag
     1981 aaacagttag ctgaaagtgg acgtttcgaa cagccaatcg ttacaaaaat cgaaccagcc
     2041 caaccatttt atctagcaga agaagaccat caagagtatt ataaaaagca tcctgaaaat
     2101 tttaaaagaa accatgcacg ccgggctgcc tttatcgaag aaaattggag gaacgaaggg
     2161 tgaatcgaag cttttttcat tatttaatga cactgcgagg cggtggcaca catgatgctt
     2221 tatctgtttt cgctacagaa gccggtaaag acatccaatt tcctaagcac agctctagct
     2281 acgaagaaat ttctaattat ctggaactga atgtcgacta tttgccgtcc atggatatct
     2341 ttgacgaagc atgggaaaaa tatttagaaa ataatcatga catataagag atagttttgt
     2401 actttatgat caaaaaagtt ttttgtccaa tagcctaatt gaaagaatta tttttatttc
     2461 a
//
LOCUS       DACDKL010000255         2446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDKL010000255 DACDKL010000000
VERSION     DACDKL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.259"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(830..1348)
                     /locus_tag="IV329_002626"
     CDS             complement(830..1348)
                     /locus_tag="IV329_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8941774.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            1715..1963
                     /locus_tag="IV329_002627"
     CDS             1715..1963
                     /locus_tag="IV329_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941775.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT          799 a          447 c          362 g          838 t
ORIGIN      
        1 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
       61 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
      121 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
      181 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
      241 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
      301 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
      361 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
      421 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
      481 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
      541 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
      601 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
      661 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
      721 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
      781 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
      841 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
      901 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
      961 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
     1021 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
     1081 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
     1141 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
     1201 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
     1261 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
     1321 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
     1381 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
     1441 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
     1501 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
     1561 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
     1621 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
     1681 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
     1741 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
     1801 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
     1861 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
     1921 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
     1981 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
     2041 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
     2101 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
     2161 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
     2221 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
     2281 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
     2341 atgctgctca tttcaactta tatgttttag tgaactaccc ccacttagct tggataggct
     2401 acaattaact agacagaaaa attaaggtgt gtagactaga agaaaa
//
LOCUS       DACDKL010000256         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDKL010000256 DACDKL010000000
VERSION     DACDKL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.260"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IV329_002628"
     CDS             5..1150
                     /locus_tag="IV329_002628"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8941776.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IV329_002629"
     CDS             1321..1779
                     /locus_tag="IV329_002629"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8941777.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IV329_002630"
     CDS             1938..>2407
                     /locus_tag="IV329_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8941778.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACDKL010000257         2390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDKL010000257 DACDKL010000000
VERSION     DACDKL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.261"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..>2390
                     /gene="esp"
                     /locus_tag="IV329_002631"
     CDS             4..>2390
                     /gene="esp"
                     /locus_tag="IV329_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8941779.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          869 a          378 c          493 g          650 t
ORIGIN      
        1 ataatggtta gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg
       61 aagtacagta tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg tgttggtctt
      121 gtcttgggaa caactgaact agttaaagca caagatgaaa tttctccaag cagcccactt
      181 gaaacagcga taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag
      241 gagaatcctg gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa
      301 ctaactggag acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat
      361 attgcagcat caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa
      421 tatgtagaaa aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt
      481 gtaagaatta atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt
      541 gcaagtgatg gattatttgc tggggcagaa acaaccgata cgcaaaccgc tcctaatggg
      601 gtaattacgt ttgaaaagag cctagatgaa atttttaaag agataggcat agacaatctc
      661 aagaccgatc gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt
      721 gtaccgggta ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc
      781 ttagatgtat ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat
      841 gagaaaccta atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata
      901 atcttagatc ataagttaac gaaaaattat aatttctcat attcatcttc cgcaaagggc
      961 acgccttggt atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata
     1021 cagatgcaca tggttcaggc tgacaaagta acatatgatg tttcgtttga atccggaaca
     1081 aaagtagctg atttagcaat cgagcgacgg gaaggtcatg aaaattatgg cataggttca
     1141 ataactaata atgatttaac taaacttatc gattttgcta atgcaagtcc acgtccagtc
     1201 gttattcgat atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa
     1261 gcaacagcac aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg
     1321 ttgtcggata cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg
     1381 caagatattg atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca
     1441 tatatcggga aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa
     1501 gtattcatcc acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt
     1561 actgtattag cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat
     1621 actgtagaat ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta
     1681 aaaatccaaa tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag
     1741 gaatcaccaa aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa
     1801 gaatcaattc ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag
     1861 cctgatactt ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat
     1921 cgaactttcg acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat
     1981 gcaacatacg tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca
     2041 tttgataaac cagatgttcc tttagctaaa gatgcctttt caatttcaga tgtctataat
     2101 aagtacttcg gtaatgcaag tgttgatgca aacactggta ttgttacatt cactccagct
     2161 aaaggtgtag gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa
     2221 gatggttctg taggcacgac cgatttagca gtaactgtaa gtaaagatat ttatgaaaat
     2281 ccaggagaaa acattcctgc aggctaccac aaagtaacct tcaccgcagg ggaaggaaca
     2341 agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt
//
LOCUS       DACDKL010000258         2366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDKL010000258 DACDKL010000000
VERSION     DACDKL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.262"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            465..1958
                     /locus_tag="IV329_002632"
     CDS             465..1958
                     /locus_tag="IV329_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8941780.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2093..>2366
                     /locus_tag="IV329_002633"
     CDS             2093..>2366
                     /locus_tag="IV329_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8941781.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT          811 a          369 c          480 g          706 t
ORIGIN      
        1 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
       61 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
      121 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
      181 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
      241 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
      301 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
      361 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
      421 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
      481 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
      541 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
      601 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
      661 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
      721 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
      781 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
      841 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
      901 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
      961 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     1021 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     1081 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     1141 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     1201 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     1261 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     1321 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     1381 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     1441 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     1501 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     1561 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     1621 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     1681 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     1741 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     1801 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     1861 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     1921 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     1981 ttatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     2041 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     2101 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     2161 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     2221 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     2281 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     2341 gtataaaata gctaacgagt taagaa
//
LOCUS       DACDKL010000259         2362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDKL010000259 DACDKL010000000
VERSION     DACDKL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.263"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            350..967
                     /locus_tag="IV329_002634"
     CDS             350..967
                     /locus_tag="IV329_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941782.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1008..1307
                     /locus_tag="IV329_002635"
     CDS             1008..1307
                     /locus_tag="IV329_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941783.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1341..1508
                     /locus_tag="IV329_002636"
     CDS             1341..1508
                     /locus_tag="IV329_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8941784.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1553..2158)
                     /locus_tag="IV329_002637"
     CDS             complement(1553..2158)
                     /locus_tag="IV329_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941785.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          802 a          369 c          438 g          753 t
ORIGIN      
        1 aatatatagt gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca
       61 aattctgatg tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta
      121 gtttatttaa tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata
      181 taatcatgcg ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat
      241 tttctttgtt tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta
      301 atctgattgt tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt
      361 tttttatatc cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa
      421 ggaattagga atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa
      481 acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca
      541 atcattagat agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac
      601 taaggaaaag cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta
      661 caagcaacta gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta
      721 tatggccgaa gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc
      781 gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc
      841 gatctattct gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg
      901 catgaaacga aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt
      961 ttcttgatag atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga
     1021 atcaaagggt cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt
     1081 tggttcactc tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg
     1141 tttgtggtca gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa
     1201 ccgcgcggga cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt
     1261 atgcgttaat tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat
     1321 ttacaaggag gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa
     1381 ctagttttaa tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa
     1441 gtgtttatca aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg
     1501 gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat
     1561 cattcgcttt ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct
     1621 ctgcaactgg aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt
     1681 gcggatctgt tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt
     1741 gagccaataa tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa
     1801 tataacttag cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag
     1861 tattaaaatc taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa
     1921 aagcaactgt attttttata tcttcataat ctcttcctaa acgatctagt gatgtaacga
     1981 tgacgcaatc accttcacgg atataattta gtaatttttg aaactcagga cgctcagtat
     2041 ctttaccact ggctttttct tggaataatt tatcaatgtt aagtccttcc atcattgcca
     2101 attgtcgatc caaattttga tcttgtgaag atactcgaat atagccaatt tttcccataa
     2161 aaaactcctt tttaaaagtg atataattta catgtaaaat ttacttgtag ttttactgct
     2221 atcatagcat ttttaaaaat aaattttatg tagacttaaa atttacttca tggagggttt
     2281 aaatggatga aatcaaaagt aacaaaaaag catttggcat ttatttagga gaacctagta
     2341 cagaacaatt aaaccaattt tt
//
LOCUS       DACDKL010000260         2330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDKL010000260 DACDKL010000000
VERSION     DACDKL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.264"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..452
                     /locus_tag="IV329_002638"
     CDS             <1..452
                     /locus_tag="IV329_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8941786.1"
                     /translation="NIYFELYHHRAIYTNEDALVIKKEQGFTGTETKSLFLKDKKDNN
                     YVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDE
                     ELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            489..1499
                     /locus_tag="IV329_002639"
     CDS             489..1499
                     /locus_tag="IV329_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8941787.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1614..2327)
                     /locus_tag="IV329_002640"
     CDS             complement(1614..2327)
                     /locus_tag="IV329_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941788.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT          783 a          382 c          491 g          674 t
ORIGIN      
        1 aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa gatgctttgg
       61 taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc ttaaaagata
      121 aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat ttcaaagcat
      181 tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg gaagaacaaa
      241 cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt ccgattatta
      301 ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa
      361 cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag atgccgatat
      421 ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat acttggaggt
      481 gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg gttactgcac
      541 catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta aagagaatgg
      601 aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat aaggaaaaat
      661 caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc
      721 aaatgatctt tccgacttgg ggaggagaat tattgattga gattctcgat tttgtcgatt
      781 ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg ttgctacttg
      841 ctgtcacatt aaaaacaggg atagccactg cacatggaac taaccttatt gatttaagag
      901 gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca agagaaggag
      961 aatcgattct acagcaatct tctgaaaagt atcaaaaagt gtggccccat gagcattcat
     1021 catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc ggagatgcag
     1081 taaggatgaa gggacgatta ttaggtggtt gtattgatac gatcaggcat ttaatcggta
     1141 ctccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt
     1201 ggtttctaga aaactgtgag ctatcgtcag ttgatttaag aagatcactt gttcagatga
     1261 aactggcagg ctggtttgat ggcagctcgg ggatcatgtt cggtagaagt gcggcagagg
     1321 atttgttaga ttataaaatg aaggatgtat acgaagatct gtcaaaggaa ttaaaactac
     1381 cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg attaatggtg
     1441 cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg tttatttaaa
     1501 aaattttttc ctgccttgga gtctatcaaa gaccaataaa tctaaataga aaagaaagtg
     1561 aaagaagctg acaaaatgct gtcagcttct ttttgtataa catattagaa agtctaatta
     1621 tctataactg tcacatcgat taactgtttc acgtcattgg gatttcctgt aggaatagct
     1681 actacagatg cattatcaat gatttcttga gttgtttctc taatgaacgc tggataagat
     1741 gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat tgtcccgtca
     1801 tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag cataaccaca
     1861 tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc tgcaggataa
     1921 tgaataacat gtaattcgaa atcacccgat gtatctctaa aagtattacc tctaatagct
     1981 agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg atctattgac
     2041 tgagaagact gtgaggaggt tgtgtctatt gttgaagaag aaatatttgt ctcaactgta
     2101 ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga agatgtattc
     2161 gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt ttgtctgatt
     2221 gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga cgtacatcct
     2281 acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata
//
LOCUS       DACDKL010000261         2307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDKL010000261 DACDKL010000000
VERSION     DACDKL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.265"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..1168
                     /locus_tag="IV329_002641"
     CDS             50..1168
                     /locus_tag="IV329_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941789.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1671..1991
                     /locus_tag="IV329_002642"
     CDS             1671..1991
                     /locus_tag="IV329_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941790.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT          767 a          385 c          477 g          678 t
ORIGIN      
        1 agactttaga tagaaacaaa ggaatgattt ttttaggagg ggtacgttta tggcaaaaaa
       61 ggataaagaa ttattgtcac aattgacttc agaaggcgct aaaggaccat tcgtcacgat
      121 catgctgaat acgcatgtcg cccatcaaga tgtggaaaaa gatcagatca aattcaaaaa
      181 ttttgcgaaa gaagcaaaga aacgttttga gaaaaaatat tccgaacatg actggtcccc
      241 gttccaagaa aaaattgaaa atttgttagg agatccttcg ttttggcgta gtgcaactac
      301 gagtgtagcg gtcattataa ctgctgacca aacctatatc catcgtttga gtatcccggt
      361 agacgatcaa tactatgtaa gtgatcttcc ttatttactg gctattatca aaaatgggca
      421 gttcaactat tcttattatt tattgggatt gaaccgcgac tccatgaaat tgtatgaagt
      481 cagcaacaaa caagtaaaag aagtatctct accagaaggg gcaccggttg atgtcgttac
      541 tgcattggga tctgaactga caggtggcgg tttgaacttc tcttctaatg gaggaggcgg
      601 agcccgagat ggagtaactt tccatggcgt aaatccaaaa gatgaagaag tagagatcga
      661 ttgggtcaac tattaccaag cagttgatac gtacttgaag gattctttcg aaaatccaga
      721 acaatggccg atttatttat ttgctctacc agaaaatcag acgatgttca aaaaaattgc
      781 taaaaatccc tattattgtg cagacgttgc gatttctgct tctcctgcgc agctatcctt
      841 tagagatatc gaagaagggg cgaagaagct gtcaagtgaa ctcgcagctc aagaatcaaa
      901 agaatacaat aaactcttag ataagaaatt catcgatcag ttagttgata tcgttccagc
      961 tgcgaaagaa ggaaaagtgt cacatttatt tatcgctacg tcaaacttag ctgatggttt
     1021 cggtgaagat cctgaaacag aatatgatcg gcgtcaagtg ttgaatgcat tagcagatga
     1081 tgtgatcatt aatggcggaa aagtgtttat tttggatcaa aaagatgcgc cagatgaaaa
     1141 gagtctgaca gcgattcttc gttattaaaa gaggttgtga aatcagtgtt ttgacctgag
     1201 tattagagaa acaaacggaa aaatagtttc acatattttg ccgttttttg agctaatacc
     1261 gaaggtcagc tgattaaaca ccgtgtatga gaggttgtga aaaagctgtt ctgcatcaag
     1321 taataagaac agcaagcaaa aatagctctt catatttttt gtttggtggg cttattaccg
     1381 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gacagaagtg gtcagcttca
     1441 agaaataaga agagattgac aaaaattatt tctcaaattt ttgtgaaatt tcggcttatt
     1501 tccgaagaag ctacttctgg aacaccgttt aaaaaagagg ttgtgacgtg gtcacgacct
     1561 ctttttgacg attatgatac aaaaaacatc atttatgaac aaaatgtgaa taacgggcgt
     1621 tcttgagata tgattttaat tgggaaaaac ttatcgataa ggaggtcttt atgaaaaaaa
     1681 gactgttatg gtgtttgctg tctctatccc taagtttaac aagtcagcca gtttttgctg
     1741 aagaaacaca gcggtttcct attacaggca cacgtctgta caaaaatgtt tatgtacaga
     1801 caccagatct tgattctgcg attcctgaaa cagaagaacg gtctttgcag ccaagatttg
     1861 gttacttttg gtactgtccg acctgtgatt atgaaagttg ctggcatttc ttacattcta
     1921 cagctgcagc ttttgctttt actcatactc agaaatatga tggacatcat cctgttattt
     1981 atgaggtata gatatgagca aaaaagaaaa actaatctcg ttggtcgtat tggtttgcct
     2041 tgttcttctg gtaactgtca taggctatca gaaataccat gagaacatca gacctactaa
     2101 agaatcttcc ccagcctcga ccgttgaaac agaaccgata agtagtcaga aagaataatt
     2161 tttttagttt aaaaaagaac atcagagcaa aaaaacacca actgaacaat atgtcgttta
     2221 ttttaaaaaa aagttcggtt tttcacttta tgtaatcgct gagaaaactt tttttaataa
     2281 aaaaagtcga aaaactctag ctttttt
//
LOCUS       DACDKL010000262         2290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDKL010000262 DACDKL010000000
VERSION     DACDKL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.266"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            97..1959
                     /locus_tag="IV329_002643"
     CDS             97..1959
                     /locus_tag="IV329_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941791.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1959..>2290
                     /locus_tag="IV329_002644"
     CDS             1959..>2290
                     /locus_tag="IV329_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941792.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          823 a          341 c          451 g          675 t
ORIGIN      
        1 ttaaaaagct ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag
       61 aaaacgctta tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa
      121 aggcagcttt tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct
      181 gaaaaagaaa atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg
      241 ctgaatgagc ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt
      301 agtagctctg atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata
      361 tttgcagatc aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa
      421 gctattactg gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat
      481 gatttagatg tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg
      541 cgaaagggtt tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt
      601 cttcaaaaag agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc
      661 ggtgagactg aatactttga aatgtattct gctactgcta cacctatcca agaggtattt
      721 aatcaagtaa tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat
      781 tacgcggaat tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa
      841 gaggctacga ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt
      901 tcttatcgcg tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa
      961 tatcattata tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta
     1021 gttgagaatg tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca
     1081 caattgttga cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt
     1141 gtcaatgaat ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat
     1201 gcgatcaagc aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct
     1261 ttcgtggcat atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga
     1321 agcgttagag ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa
     1381 aagatcaatg aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg
     1441 caagaaatga ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc
     1501 atcgatcgtc ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt
     1561 caacaaataa taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat
     1621 cttagagaaa cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg
     1681 aaagcttata cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa
     1741 tatcgagaag cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa
     1801 caatatatgg cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta
     1861 ggaaaaatcg aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt
     1921 gcattattct catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt
     1981 taaaaattat cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg
     2041 aagagcgatt agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta
     2101 atcacttgaa tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc
     2161 gggatttgtt caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa
     2221 gtatcggtaa tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa
     2281 aaaataatta
//
LOCUS       DACDKL010000263         2248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDKL010000263 DACDKL010000000
VERSION     DACDKL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.267"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..83)
                     /locus_tag="IV329_002645"
     tRNA            complement(12..83)
                     /locus_tag="IV329_002645"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Glu,seq:ttc)
     gene            complement(95..168)
                     /locus_tag="IV329_002646"
     tRNA            complement(95..168)
                     /locus_tag="IV329_002646"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Ile,seq:gat)
     gene            complement(181..251)
                     /locus_tag="IV329_002647"
     tRNA            complement(181..251)
                     /locus_tag="IV329_002647"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Gly,seq:tcc)
     gene            complement(256..328)
                     /locus_tag="IV329_002648"
     tRNA            complement(256..328)
                     /locus_tag="IV329_002648"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Phe,seq:gaa)
     gene            complement(332..404)
                     /locus_tag="IV329_002649"
     tRNA            complement(332..404)
                     /locus_tag="IV329_002649"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Asp,seq:gtc)
     gene            complement(423..496)
                     /locus_tag="IV329_002650"
     tRNA            complement(423..496)
                     /locus_tag="IV329_002650"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Met,seq:cat)
     gene            complement(509..598)
                     /locus_tag="IV329_002651"
     tRNA            complement(509..598)
                     /locus_tag="IV329_002651"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(560..562),aa:Ser,seq:tga)
     gene            complement(621..692)
                     /locus_tag="IV329_002652"
     tRNA            complement(621..692)
                     /locus_tag="IV329_002652"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Glu,seq:ttc)
     gene            complement(714..801)
                     /locus_tag="IV329_002653"
     tRNA            complement(714..801)
                     /locus_tag="IV329_002653"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(765..767),aa:Ser,seq:gct)
     gene            complement(826..899)
                     /locus_tag="IV329_002654"
     tRNA            complement(826..899)
                     /locus_tag="IV329_002654"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(863..865),aa:Ile,seq:gat)
     gene            complement(913..983)
                     /locus_tag="IV329_002655"
     tRNA            complement(913..983)
                     /locus_tag="IV329_002655"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Gly,seq:tcc)
     gene            complement(985..1060)
                     /locus_tag="IV329_002656"
     tRNA            complement(985..1060)
                     /locus_tag="IV329_002656"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1025..1027),aa:Asp,seq:gtc)
     gene            complement(1079..1152)
                     /locus_tag="IV329_002657"
     tRNA            complement(1079..1152)
                     /locus_tag="IV329_002657"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1116..1118),aa:Met,seq:cat)
     gene            complement(1165..1254)
                     /locus_tag="IV329_002658"
     tRNA            complement(1165..1254)
                     /locus_tag="IV329_002658"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1216..1218),aa:Ser,seq:tga)
     gene            complement(1278..1351)
                     /locus_tag="IV329_002659"
     tRNA            complement(1278..1351)
                     /locus_tag="IV329_002659"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1315..1317),aa:Ile,seq:cat)
     gene            complement(1365..1438)
                     /locus_tag="IV329_002660"
     tRNA            complement(1365..1438)
                     /locus_tag="IV329_002660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1402..1404),aa:Met,seq:cat)
     gene            complement(1466..1538)
                     /locus_tag="IV329_002661"
     tRNA            complement(1466..1538)
                     /locus_tag="IV329_002661"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1503..1505),aa:Ala,seq:tgc)
     gene            complement(1558..1631)
                     /locus_tag="IV329_002662"
     tRNA            complement(1558..1631)
                     /locus_tag="IV329_002662"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1595..1597),aa:Pro,seq:tgg)
     gene            complement(1641..1714)
                     /locus_tag="IV329_002663"
     tRNA            complement(1641..1714)
                     /locus_tag="IV329_002663"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1678..1680),aa:Arg,seq:acg)
     gene            complement(1725..1810)
                     /locus_tag="IV329_002664"
     tRNA            complement(1725..1810)
                     /locus_tag="IV329_002664"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1774..1776),aa:Leu,seq:taa)
     gene            complement(1827..1898)
                     /locus_tag="IV329_002665"
     tRNA            complement(1827..1898)
                     /locus_tag="IV329_002665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1864..1866),aa:Gly,seq:gcc)
     gene            complement(1910..1982)
                     /locus_tag="IV329_002666"
     tRNA            complement(1910..1982)
                     /locus_tag="IV329_002666"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1947..1949),aa:Thr,seq:tgt)
     gene            complement(2000..2081)
                     /locus_tag="IV329_002667"
     tRNA            complement(2000..2081)
                     /locus_tag="IV329_002667"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2045..2047),aa:Leu,seq:tag)
     gene            complement(2089..2161)
                     /locus_tag="IV329_002668"
     tRNA            complement(2089..2161)
                     /locus_tag="IV329_002668"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2126..2128),aa:Lys,seq:ttt)
     gene            complement(2165..2237)
                     /locus_tag="IV329_002669"
     tRNA            complement(2165..2237)
                     /locus_tag="IV329_002669"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2202..2204),aa:Val,seq:tac)
BASE COUNT          599 a          627 c          536 g          486 t
ORIGIN      
        1 agtatcttcc atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt
       61 cttaaccgct tgaccaacgg gccaataaaa taaatgggcc taaatggact cgaaccatcg
      121 acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat aaaaactaaa
      181 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa
      241 actacacccg cataatggcg cgggacagaa tcgaactgcc gacacatgga gcttcaatcc
      301 attgctctac caactgagct accgagccaa acggttccga cgggaatcga acccgcgatc
      361 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccaatgtt tttagttttt
      421 aattgcggga gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccag
      481 actgctccat cccgcgataa tatgaaaaag gaggataagg gattcgaacc cttgcacggt
      541 tttacccgcc tgacggtttt caagaccgtt cccttcagcc ggacttgggt aatcctccat
      601 gtaaaactat attaaattaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
      661 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaaa caaaatattg aaacggagaa
      721 ggagggattt gaaccctcgc gccggtttcc cgacctacac ccttagcagg ggcgcctctt
      781 cagccacttg agtacttccc caaaaccaat aagattttgt actaatgggc ctaaatggac
      841 tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc tataggccca
      901 taaaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg aaggctgtgg
      961 ttttaccact aaactacacc cgcatggcgg ttccgacggg aatcgaaccc gcgatctcct
     1021 gcgtgacagg caggcatgtt aacccctaca ccacggaacc agtgttttta gtttttaatt
     1081 gcgggagcag gatttgaacc tacgaccttc gggttatgag cccgacgagc taccagactg
     1141 ctccatcccg cgataatatg aaaaaggagg ataagggatt cgaacccttg cacggtttta
     1201 cccgcctgac ggttttcaag accgttccct tcagccggac ttgggtaatc ctccatatga
     1261 aacttacaac gccacaatgg accttgtagg actcgaacct acgaccggac ggttatgagc
     1321 cgtctgctct aaccaactga gctaaaggtc caggctctta aaaatcgcgg cggaggggat
     1381 cgaacccccg acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgcgaa
     1441 aaagatattc attatttaaa tgaaatggag cctagcggga tcgaaccgct gacctcctgc
     1501 gtgcaaagca ggcgctctcc cagctgagct aaggccccgt gtttaaattt ataattatcg
     1561 ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga
     1621 gctacttccc gtttaattaa cgcgcccaag aggagtcgaa cccctaacct tttgatccgt
     1681 agtcaaacac tctatccagt tgagctatgg gcgcatattt gaaatgccga ggaccggaat
     1741 cgaaccggta cggtgatcac tcaccgcagg attttaagtc ctgtgcgtct gccagttccg
     1801 ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg ttcgaacccg cgacccccac
     1861 cttggcaagg tggtgctcta ccactgagct atttccgcgt gtttaaagat gccggctaaa
     1921 ggacttgaac cctcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg
     1981 gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac ccccacgccg taaggcgcta
     2041 gatcctaaat ctagtgcgtc tgccaattcc gccacacccg caaaaatatg agccgtacag
     2101 ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc taccaactga gctaacggct
     2161 catatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
     2221 agctgagcta aacctccatg gataactt
//
LOCUS       DACDKL010000264         2234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDKL010000264 DACDKL010000000
VERSION     DACDKL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.268"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV329_002670"
     CDS             <1..544
                     /locus_tag="IV329_002670"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8941793.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IV329_002671"
     CDS             complement(900..1472)
                     /locus_tag="IV329_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941794.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IV329_002672"
     CDS             complement(1488..2093)
                     /locus_tag="IV329_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8941795.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          339 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ctag
//
LOCUS       DACDKL010000265         2212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDKL010000265 DACDKL010000000
VERSION     DACDKL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.269"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            123..389
                     /locus_tag="IV329_002673"
     CDS             123..389
                     /locus_tag="IV329_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8941796.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            389..2116
                     /gene="ptsP"
                     /locus_tag="IV329_002674"
     CDS             389..2116
                     /gene="ptsP"
                     /locus_tag="IV329_002674"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8941797.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT          761 a          381 c          491 g          579 t
ORIGIN      
        1 tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt aggattctct
       61 gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg agaccgatta
      121 atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct cgtccagcta
      181 cactattagt acaaacagca agcaaattta actctgatgt aaacttagag tataaaggta
      241 aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt caaggttctg
      301 atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca atcgttgaaa
      361 caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga ttgccgctag
      421 tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa cattcagtaa
      481 ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat tagctaaatc
      541 tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg aagcagaagc
      601 gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag gtcaaatcaa
      661 gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag taacggatat
      721 gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc gtgctgcaga
      781 tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt taccaaatcc
      841 atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa gtgatacagc
      901 acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac gtacttcaca
      961 ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa aagaaattac
     1021 tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg atgtaatcat
     1081 cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt acgcagcaca
     1141 aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg gcaagcattt
     1201 tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata ataatggtgg
     1261 agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag acttcccaac
     1321 agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag gaaaacctgt
     1381 agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc aattacctca
     1441 tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg aacaaggcga
     1501 cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg gtaatttacg
     1561 tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag cgatctttga
     1621 agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga tccaagtcgg
     1681 catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa aagaagtaga
     1741 tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag accgtatgaa
     1801 cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt tgatcaaaaa
     1861 tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag aaatggctgg
     1921 agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct caatgagtgc
     1981 gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga aaatggctga
     2041 acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg aacttgttca
     2101 tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag agctacgcca
     2161 gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa aa
//
LOCUS       DACDKL010000266         2190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDKL010000266 DACDKL010000000
VERSION     DACDKL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.270"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            230..604
                     /locus_tag="IV329_002675"
     CDS             230..604
                     /locus_tag="IV329_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941798.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            609..1037
                     /locus_tag="IV329_002676"
     CDS             609..1037
                     /locus_tag="IV329_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8941799.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            1128..2042
                     /locus_tag="IV329_002677"
     CDS             1128..2042
                     /locus_tag="IV329_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8941800.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
BASE COUNT          807 a          352 c          381 g          650 t
ORIGIN      
        1 gttgacaata actaatattt ttattagtat atagacatag ctaaataaga cacagaaatg
       61 cctagtaatt tacatttcag agtttgccga ccgtgaagtg tttattagtt atcaagtctt
      121 tttagctaat attttagctt acgaaaacag tatactaaca cttttattag ttgtcaacac
      181 ctttaactaa tttttttatt agtgttttcg tagcttgtga ggtgcgaaaa tgaatttact
      241 agatagaata aaggaattag ctcataaaag aggcataagt ataactcaat tggaagaaga
      301 attaaatata cctaaaaaca cgatttatca atggaaaaat agaactccta gtactaaaag
      361 actacaactt gtagcagatt attttaatgt aactactgat tatttactcg gtagaaacca
      421 agttcctgac tgggcaacaa aagatgaagt agttgaactt gataaattac tagattcaaa
      481 tgtcaatatg tcttatggcg gagaaacact gacgcctgaa caggtccaac gcgtaaaaga
      541 tatccttatt ggaaccttct gggatattgt gaaagaagac aaagaaaaag gcaaaaagat
      601 gtgagcttat ggagatggat acgattaatt tagtcgagga gttgaagcgg aaataccagt
      661 ccgctaatcc tttttatatt tgtgaaaaga tgggcattaa aattcaatac gttcctttta
      721 tcgagaatcc caaaggacag tttcaagaaa ttagaggtcg tgcaataatc ttcttaaatg
      781 atgaactgag agactctgag gaaagattct acatttgcgc tcacgaatta ggtcacgcta
      841 tttttcatcg tggcttatcc agttactatg tatcaacaag aacatctaga agcaaatctg
      901 aaagcgaagc taattgcttt gctgctaatc tcattgtttc tctatacaaa gaagacaatg
      961 atcaatatcc taaacgaatt gaggaattaa agaatcttta cggacttcca gaaagcgctt
     1021 atcgctttct tatataatgt aaaaaaagtc cgtgctgcaa cacggactaa aaaccttatt
     1081 tctaagatcc tacaaaaaaa tcatatcata gaagtgagga attaaaaatg aaaaaaattg
     1141 tttttgggtt gcttttttta ggcttggtat taagcggatg ctccactagt cctaattctg
     1201 ctagcactaa agaaaatact gtttcatcaa catctgaata tgaaacaaaa aaactaactt
     1261 catcagacgt tcataaaata aaagttggtg atgatttaac tcaagtttat cagacacttg
     1321 gttcaccgtt acgtaaatgg acaaatgatt ttgtttacaa agaattagac aatgctatca
     1381 cacgggatga attaattgta tctatttcaa aggatgcaca aggagattat tataaagagc
     1441 actctgaatt aattgaacat ggaaatagtg caaaagatat aaaaaaatta gagatgctcc
     1501 aatatactta ttcaactgat tctgatgatg aaacaatgtt aatttgggtt agtccaaaaa
     1561 ctgaacaggt tgtatattat aatcaacgaa ctttcttaga taaagatgga aaatccaaag
     1621 attcaagctc tactacaact acaactacaa cttcgtcttc agaaccccag acggcatcag
     1681 ttgcacatag tataggagaa gcagttcctt ttacttctga aaatggtaac tctattaacg
     1741 taactgtcca gtctgtatca aaagattacg gtgatgattt ctataaacca gaaggcctat
     1801 tttacgcaaa ggtagatttt cttgtagaaa acacagggac tacacctttt gatgcaagtt
     1861 caagttattt agaattttat gattcatcag atattaaatc tgatttagct agtagagatt
     1921 acttttcgga aaccatccaa ccgggtaaat ccgcacaagg aacagcatat tttgaagtga
     1981 aaaatgatgg aacaacattt gaagtgttct tcgcagatac ttcatggaaa ggtagctact
     2041 aaacaaaaaa acacgccccc tccctcgcca aagtttgtgg acgtgatgaa aaataaacct
     2101 gctataattg gcttacttat ccattcctat tatagcaaca aataggagat gaaaacaatg
     2161 tggattgaaa ccaaaactga taaaaacgga
//
LOCUS       DACDKL010000267         2185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDKL010000267 DACDKL010000000
VERSION     DACDKL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.271"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(367..1068)
                     /locus_tag="IV329_002678"
     CDS             complement(367..1068)
                     /locus_tag="IV329_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8941801.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1084..1524)
                     /locus_tag="IV329_002679"
     CDS             complement(1084..1524)
                     /locus_tag="IV329_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8941802.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1524..2099)
                     /locus_tag="IV329_002680"
     CDS             complement(1524..2099)
                     /locus_tag="IV329_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8941803.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          657 a          456 c          328 g          744 t
ORIGIN      
        1 aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg cgacaactgt
       61 cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca aaattatgta
      121 aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt tttatgaact
      181 tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt ggatcatttc
      241 agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat aaaataacca
      301 aacgatagaa aggcttattc tacatcacgt agtaattttt ctttcccgtc gtttggtttt
      361 ttatttttag cctaattctt gaccttttgc atgctggtta tccgtaaaat attttccagc
      421 aggctttcct tccaattcag ctaatttttc ttggatctga cggatcacct tttttgcatc
      481 ttctggattt tctacaaaat cgaatttatc cccatccact tgtattttag gacatacatc
      541 aaaatcttca taccattgat tatagcgggt gttcaacgtt tcataatagg tatataacgt
      601 ctcatcgctt tctagctgtt catagtcacg cccacgtttt ttgatacgtt ctagcatcgt
      661 gtccaaagaa actttgatat ggaccatcaa atcaggccgt ttttttggag caacatcatc
      721 taactcgttt agcatcgtat ctaataagct atcgtactgc tgtacttcga tatccgttac
      781 ccgacctaaa tcagcgttta gatggaacaa caggctatct tcatagatgg atcggtctag
      841 cacattgtca tcgttcacta aagcattttt cattgctaaa aatcgcttgt tcaaaaagaa
      901 gatttgcaat aaaaaagcgt actgctcagg atttgaataa aagagcggca atacttcgtt
      961 atcttcgaca ttttcataaa atggtttaga atcaatctct tttgcaagca tatccgtcaa
     1021 actgctctta cctgcaccaa tcgttcctga aatcaaaatc actgacatac aacccttcct
     1081 cttctataag tactcgccta cgtaagtttc aacaggcaat gtttcaaaat catattcacg
     1141 tacagcttgg tctgatttta aatacgtatg gagactttgt gtaatcatca agttcgtagg
     1201 taccgtctct tcttgtacaa tgatcatcgg ttttttcaac atagttgcca cacctaattc
     1261 gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca aggatagcaa caacaaattc
     1321 tgcatttgtc agttcttcca tatctttttc gtatacttcc tgcgcccatc cagcgctgaa
     1381 tagttcataa ttgctttctt ggtgatgacg tggagagtag aaacttgtga ctgttttgtt
     1441 ttcctccaac gctttttcga tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg
     1501 tcctgctaaa tatatttgac tcattatttt gcctccttca ataataactg atttttaata
     1561 gatactgctc gtaattcatt gaccagttcc aatggattgt tggaaaagat tacttgatcc
     1621 aaccattttt tatccataaa atcctctttt gcaaaagttt ctaataattg ttgcaagccg
     1681 ctgtaataac cgccaatatt caatattccg attggcttgt cgggcgtcag accgattgct
     1741 atccaagaaa gaagttgtga gaattcctcc aatgtaccta gacctccagg gaaaacgagg
     1801 acagcatctc ccttttcaaa taataatacc tttctttcat cgatcgtttc tgtaggaata
     1861 aatgtagtaa catcttttcc aggcaattca gtttcaaaaa gcccattcgg atagatacct
     1921 gtgacttttc cgttattttc cagtactgtt ttactaatca ctcccataat cccggagtat
     1981 gaaccaccgt aaaccagttc gatttcttct tgtgccatgt attttcctaa cgtttgcgca
     2041 ataaatttgt aactttcttt attaccaaaa cgggaaccgc aaaaaacagt aagtttcata
     2101 aaaaaacact ccctaactca aaagtctatc ttcattctac acgtgttttt ttaaaataaa
     2161 agactagatt ttgtagtttt ttagt
//
LOCUS       DACDKL010000268         2184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDKL010000268 DACDKL010000000
VERSION     DACDKL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.272"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..>2184
                     /gene="acm"
                     /locus_tag="IV329_002681"
     CDS             54..>2184
                     /gene="acm"
                     /locus_tag="IV329_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8941804.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTIC"
BASE COUNT          857 a          345 c          427 g          555 t
ORIGIN      
        1 gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa
       61 agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca tcactgattc
      121 ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact tcgttgaccg
      181 tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg tttgacgaaa
      241 aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt gaaggaaata
      301 ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa aatgttggaa
      361 ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag
      421 actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc gataccggtg
      481 aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact gtaaatgtga
      541 caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga gacatgctac
      601 ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc acttatgttg
      661 aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat ccaagtacgt
      721 ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa gagggaatcc
      781 aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact gataattaca
      841 ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg agttataaga
      901 cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct tggtttaaag
      961 aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa aatatttcag
     1021 cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa tatatcaatg
     1081 acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat gaacagccta
     1141 ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct agtatcaaag
     1201 gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg attgattttg
     1261 atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca gagggagtca
     1321 gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa atttggaacg
     1381 gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt acaaacaatt
     1441 gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact tcagtgactt
     1501 gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag
     1561 tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa aacgggctag
     1621 tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca tgggaagaca
     1681 atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta gccgacggtc
     1741 aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc
     1801 tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac aacgtgcccg
     1861 aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa
     1921 atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag aagacagaga
     1981 gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat acaggagaat
     2041 ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag ataacaaata
     2101 aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct gtaatcattg
     2161 gtttattgat agtaactatc tgtg
//
LOCUS       DACDKL010000269         2161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDKL010000269 DACDKL010000000
VERSION     DACDKL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.273"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..601
                     /locus_tag="IV329_002682"
     CDS             83..601
                     /locus_tag="IV329_002682"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8941805.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            729..1412
                     /locus_tag="IV329_002683"
     CDS             729..1412
                     /locus_tag="IV329_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8941806.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1721..1794
                     /locus_tag="IV329_002684"
     tRNA            1721..1794
                     /locus_tag="IV329_002684"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1755..1757,aa:Arg,seq:tct)
     gene            1953..>2161
                     /locus_tag="IV329_002685"
     CDS             1953..>2161
                     /locus_tag="IV329_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8941807.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGA"
BASE COUNT          738 a          329 c          438 g          656 t
ORIGIN      
        1 catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa gaaggattga
       61 ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta taagttctat
      121 aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc gaatttttgg
      181 tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac aaggaaatct
      241 gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta ttacatatta
      301 tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa acaaccacga
      361 agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag taaaaaagag
      421 aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc tttcaaaaac
      481 ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa caggaatcac
      541 agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga aatttttgta
      601 agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa catgaaacac
      661 gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt gtgaagagga
      721 ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa ctattggatt
      781 ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa gaagaagctc
      841 atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa tttttacttg
      901 gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt tcatcaagtg
      961 tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt ttcgatgtca
     1021 tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa gtgactttgc
     1081 ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac tttattttta
     1141 tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt ttgttaagaa
     1201 aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg ctcccagatg
     1261 aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt cttcgtgttg
     1321 tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt gtgatcttaa
     1381 tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc tgtagataat
     1441 cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt gtaagtcaat
     1501 taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta gctcctgttt
     1561 tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga ttgatttagt
     1621 agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat tgacagaagc
     1681 aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag ctcagctgga
     1741 tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt gacgctaaaa
     1801 aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca atattgtttg
     1861 ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata aggagcaaaa
     1921 aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga attttagtga
     1981 ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga actgattctg
     2041 acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa tctgttcaaa
     2101 cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta ctttatggag
     2161 c
//
LOCUS       DACDKL010000270         2149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDKL010000270 DACDKL010000000
VERSION     DACDKL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.274"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IV329_002686"
     CDS             complement(<1..914)
                     /locus_tag="IV329_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8941808.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IV329_002687"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IV329_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8941809.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          727 a          380 c          414 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatatt
//
LOCUS       DACDKL010000271         2149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDKL010000271 DACDKL010000000
VERSION     DACDKL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.275"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(31..>2149)
                     /locus_tag="IV329_002688"
     CDS             complement(31..>2149)
                     /locus_tag="IV329_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8941810.1"
                     /translation="EKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGTSAFGLKKT
                     FEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNVLWIADPAN
                     GKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGLVFHIVLAS
                     IFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYSRSYLLIIL
                     GQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASATLSIFLDIG
                     MVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGAAVNSSIIE
                     SLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQLISSALIL
                     WLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNEIFAID
                     SEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIVGVSGS
                     GKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTIIENLI
                     FGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLALARALL
                     KNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILVLHEGH
                     LVEKGTHDELRFADGIYQKLWEI"
BASE COUNT          729 a          390 c          312 g          718 t
ORIGIN      
        1 ttattattct gcataaattg tttccctctc ctagatttcc cataattttt gatagatacc
       61 atcggcaaaa cgaagttcat cgtgcgttcc tttttctacc aaatgccctt catggagaac
      121 taaaatttga tcacatgctt ttgctattgg taaatgatgc gcaataaaaa taatcgtttt
      181 ttcttttaaa ttgaggagat aattcataat tgcatgttct aacaaggtat ctaatccact
      241 agttgcttca tctaaaatta atatgtccgg attttttaat aaagcccgtg ctaaggctaa
      301 tcgttgtcgt tgccctcctg aaagattatt tccaccttct tccaacaatg tctcgtaacg
      361 taatggctgt tgattaataa attcatttag ttggactatc tcacaaactt ctattatttg
      421 ttcgaaactt ggtgggctat ctaaaccgaa tataagattc tcaatgattg ttccactaaa
      481 gaaaaatgac tcttgaggca aatatgtcac tcgatcgcgt aatatctgaa aaggaatgtc
      541 taagtgatta atgtttccat aatggatcga tccttctgat gcttcataaa aacgaacaaa
      601 taactttgca agagttgatt ttccagaacc actaactcca actatagccg ttttactgtg
      661 cgaaggaatt gtacatgaaa tttcgttcaa cgttggggcc ttcatgctat acgagaatga
      721 aacgtttttt acagtgatat cttgttgaaa taattgttca gaaagcatat attcaaaaga
      781 ctggttcgat ttttctgaat caatcgcgaa aatttcattt aaacgattat ttgcaacttg
      841 agccgtttgc attttcactt gtaaattaat aatgttttgt aaaggatctg tcaaaaatac
      901 tagtaaagca ttgtatgtga taagttgacc tagactaatt ctattatcca taacaaaata
      961 agaacctagc cataaaatta atgcactgct aattaattga atcaaatgtt taattccttg
     1021 ttgaatatta tcaagcgtaa ccgtacgaaa tgattttttc attaatttaa caaattcatg
     1081 gtctatacgg ttatatactt tctcttcacc gctatatgct ttaattgttt ctattccttt
     1141 aagactttct atgatactag aatttacagc tgcaccggct gccatttctt ccctgttcga
     1201 tttttcatag cttttcacga aagcgaatat tgcaactaca taaaaaggca aagagcatag
     1261 agtgattaaa aacaacgtac tattctgaat agctaacgta accccaacaa gcaacaccat
     1321 accaatatct aaaaatattg ataacgtagc acttgctaaa gcatcaataa tcttactggc
     1381 atcaagaaaa cgagaaataa tttcccctga ttttctcgta gcaaaaaaat tcattggtaa
     1441 agttaacaca tgtttgaaat actgaagcat aattgccata ctcattcgct gtcctaaaat
     1501 aataagaaga taactacgac tatattcaaa caatacccga aataaataga cagcgatcaa
     1561 gccaatggaa attatattta aagttgaacg tgcttgatta gggatgaaaa aatcaagaat
     1621 accttgaaaa taatatgagc ttcctatgcc aaaaaacgta atgaaaatcg atgctaaaac
     1681 gatgtggaat actaatcctt tttgtttcca aattataggc aaaaaagaac ttaatccagc
     1741 tactttttct ttaataggaa tataagtttc tctaggcata ggtaataaaa gaatacctgt
     1801 ccattctttt tgaaacgctt caatcgtttt tctatttttt ccattagcgg gatctgcaat
     1861 ccatagtacg tttcccttcg ttttataaat tacaacgtag tgcatatagc tgttatcaac
     1921 caatatatgt gcaattaatg gtaagataag atctctttct ttccagatct cactatctgc
     1981 ttgaatagcc atacaatcaa aagacaattt ttcaaatgtc tttttcaatc caaatgctga
     2041 agtaccctct aaatcagtcc cagataggtc ccgtaattta tgaatcggga tatcagtttt
     2101 aaaatatttt aaaatcatcg aaaggcaagc aacaccacaa tctttttca
//
LOCUS       DACDKL010000272         2143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDKL010000272 DACDKL010000000
VERSION     DACDKL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.276"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(673..963)
                     /locus_tag="IV329_002689"
     CDS             complement(673..963)
                     /locus_tag="IV329_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941811.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1171..1863)
                     /locus_tag="IV329_002690"
     CDS             complement(1171..1863)
                     /locus_tag="IV329_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8941812.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          613 a          396 c          315 g          819 t
ORIGIN      
        1 gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta attgttaaat
       61 accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca ttatatccag
      121 aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg tcgaataaac
      181 tgccaaagac atacaatcat attgctttta aaattgacga acaagatata gattcgtttg
      241 tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg
      301 aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta cataccggta
      361 ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa tgactagtct
      421 ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg aattgatctg
      481 ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt catcacaaat
      541 acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc tcgtgtgggt
      601 tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat ttttgtctca
      661 gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg ggtatagact
      721 tttctcatat tctaccacaa ttttgggttt ccctaacaca tgataggatc tcacaacaaa
      781 ttgctttcta ttattataat aacctgtaac gactaacttc atcccttcgt ctacttcagc
      841 taggaagttc aaactatggg aagcaatcaa acaattgacc ccatttaaac tgaaacggat
      901 caacggtctg gcactaactt gcaaagtacg gatcttatct actgttccat attttttctc
      961 cataaccttc acctcaatac cattatacga acatacattc cctttgtaaa gaagaaactc
     1021 tgttttatct ttttgacttt ttgtctatta cctttcttaa aatcatattg gcacttcctg
     1081 cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt atagattact gttgcttctt
     1141 tactaattta ggtttacttg ttaagaaaga ttatttacgc aaatgttctt caacaatttg
     1201 ctgagattta tcagctgtat cttccacttc atcttttgct ttttctaact tttgaatata
     1261 ttgtttcaag cttgagtttt cagcagttgc ttcgtctaat ttcttttgtg tatctgttaa
     1321 ctgagtttct acatttgttt tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt
     1381 tacagtattt tgtaattctg aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc
     1441 tgcattcaga tgattgattt catcattttt ttggttgatc tcatctagtt tgacattgat
     1501 ttcattttgt ttgctttgta gttgtgcatc cttgctgact aattgacttt ctaagttttg
     1561 tttttctgct gccgattgat ttttttggtt ttctaattca tttacttttg tttggtattc
     1621 ttggacatct gcctttgaat cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag
     1681 atctgtaagc gcttgctctt tatctgtcaa tgtatgatca agctgtgtca gattctcatt
     1741 gattgtatca atatctgtct ctccacccca caaaataatt tcatttgcgt accgcgtgcc
     1801 tgtgtagcca attagacata gaaatgcaac gacaatccca ccagtaataa atttcttttt
     1861 catttgcata gctccttaat ccaatacttt tacatgctaa aagtaagtga tctcaaacac
     1921 aatgtcaagc gtttttaaat tacatgtgta attttaatat aaatactatc aaatactcat
     1981 ttaatcctcc ttttttatct atttaaaaaa tcttttttat agagaaaaat gcaggttaat
     2041 actttcttaa ataaatattt atggtgaaca aaaacaaaaa agcaagtttt attaaacgta
     2101 acccttttca tttcatagat tactgtattt attacaaaaa acg
//
LOCUS       DACDKL010000273         2127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDKL010000273 DACDKL010000000
VERSION     DACDKL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.277"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(86..340)
                     /locus_tag="IV329_002691"
     CDS             complement(86..340)
                     /locus_tag="IV329_002691"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8941813.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="IV329_002692"
     CDS             complement(451..705)
                     /locus_tag="IV329_002692"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8941814.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="IV329_002693"
     CDS             complement(898..1173)
                     /locus_tag="IV329_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8941815.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="IV329_002694"
     CDS             complement(1145..2098)
                     /locus_tag="IV329_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8941816.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          635 a          392 c          310 g          790 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatat
//
LOCUS       DACDKL010000274         2117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDKL010000274 DACDKL010000000
VERSION     DACDKL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.278"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            115..492
                     /locus_tag="IV329_002695"
                     /pseudo
     CDS             115..492
                     /locus_tag="IV329_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            540..1940
                     /gene="yfcC"
                     /locus_tag="IV329_002696"
     CDS             540..1940
                     /gene="yfcC"
                     /locus_tag="IV329_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8941817.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1973..>2117
                     /locus_tag="IV329_002697"
     CDS             1973..>2117
                     /locus_tag="IV329_002697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8941818.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          588 a          360 c          449 g          720 t
ORIGIN      
        1 gtcattgata tcagtcctat tcgagtatgt tttctgcaaa aaacattgta ctcctaactg
       61 agatcaatgg cttttttgat gcttcacttt tacacaatta tatggacgta atcaaaaaat
      121 gtttccacag aggacttctt taattattgt cgattagagg aaagtggtgt aacagtcact
      181 agcggtacag atttaccgct gtttattact gatattcctg aatcgattat aagagcagta
      241 ttccgtcgtt ttcctgaagg agaagaagtc tgggagaaag aaacaagctt cacgacaacc
      301 gagctgctga aaagttttac aagcaacagc ttcaaagtaa atggtttatc ggagtacgga
      361 gaaataaaag tagggaaaag cgccagctta gctgttttca gcgaggacct ctgtactgat
      421 gactggcaaa aagtgatggc tgcggaagtt atcgctacct atcttgatgg tgacttagtt
      481 tacaagaaat aatgcttttc gatataacgt tataaaacga attaaagaga ggttaaaaaa
      541 tggaaacaat gaaaagaaaa ttcaccttcc cacatagtta tacattgatt tttatcttaa
      601 ttattttggc tgcaatttta acttggataa ttcctagtgg tcagtttgag acagttgttg
      661 aaaacgtcgc aggtatggaa aaaacaacgg ttgttccggg aacctatcac caaatcgata
      721 aggcagatta tggacaaggt ttttcctcta tactaatggc accagctgaa gggattattt
      781 cagctgttga ggttgttgct tttgttctca tcgttggtgg tgccttcggg attatcctaa
      841 aaacgggagc catcgatcga gggctgttta ccttagcaaa agttctggga gacaagggga
      901 tcttagttat tccgatgtcg atgattctat tcagcttagg aggatcaacc tttggcatga
      961 gcgaggaagt cattccctta tttgcggtgt ttatttctct gatgttttct cttggctatg
     1021 attccatgac tgcgatcttg attctatttt taagtacaca agcgggatat gttgggtcga
     1081 cgattaatcc cttcaacgtt ttaatcgcac agggagtagc tggcgtttac ggaaatcccc
     1141 tgctttggtt tcggatgcta tcctggattg tcattactgg gatttcaatt gttttcacca
     1201 tgctttatgc tagcagagtc aaaaagaatc cactgtcctc tattgtttat gagagtgatt
     1261 taaaggttaa gcataagttt caaaattttg acactgaaaa agagttttca acacaagata
     1321 aactaattat cgccggtttt atcgtaggtt taggaatcat gatttggggt atcatttcca
     1381 agggatggta tatggttgaa atttctgcac tattcttagg attaggtctt tttgctgggg
     1441 gcatcgctag attttctcaa aaggaaattg ctgaagcttt tgtcgaaggc tgttcagatt
     1501 tcgcctatgc tgctgtaatc attgggctag ctagaggagt cttggtaatc ttggaaggtg
     1561 gaatgattat tgacactatt ctaaacagct tggctaacct tttaggtggt cttccaaaat
     1621 tcctgttcac tacgattcta ctcatttctc aagtaattgt ttcctttttt gttccatcct
     1681 cctcaggagc tgcggcatta acgatgccgg ttatggcacc attgggtgat ctaatgaatg
     1741 taagtcgtga tgttgttgtt ctaagtaacc agtttggaaa tggtttaatg aatattatct
     1801 ccccaactag tggtgtatta ctggcaggat tagccatcgc agagattaat ttctctaaat
     1861 ggtttaaggt tggcttcaaa atatttgcca tcttaaccct tgtttctgct gttttgttat
     1921 acattgctac actcttttag tcttaatcat ccattaatag gaggcaatag ttatgattaa
     1981 aataaatcaa ttagcaggaa tgaatatcca ctatttattt tattctctca aagaattttt
     2041 cgcttatcaa cagaaaattg gtatttcttc tgttgaactt tggggaggtg ctccgcattt
     2101 tttcatagat gctttta
//
LOCUS       DACDKL010000275         2087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDKL010000275 DACDKL010000000
VERSION     DACDKL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.280"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..1289
                     /locus_tag="IV329_002698"
     CDS             9..1289
                     /locus_tag="IV329_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8941819.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            1302..2030
                     /locus_tag="IV329_002699"
     CDS             1302..2030
                     /locus_tag="IV329_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8941820.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT          678 a          370 c          364 g          675 t
ORIGIN      
        1 tgtactttat gtttttatgg tccatagcgt tgcaatattt gatttacaca atttgtatct
       61 tattgattga ccatcacatc cgatacaaat atctgtttgc gggagtcatt ctgatttctt
      121 gtattttgat tggatgggtc tattcgattt tagggatttt tactgctcta attgctatag
      181 gaatgctgat gcttactttt ttcttctttt cgacttcgcg tcaacaattg gtttacgcgc
      241 ttccttttgg gttattgacg tcgcttttag gagatcactt aacaagcatc gacgattttt
      301 ttgttttgaa atcttcggtt attgaaccag gagattcatt gcagtatttt cacattgtct
      361 tatccgcttt tttatctgct ttattagctt tttgtttaaa aaaatcgtta gagacttggt
      421 ttcacactgt ggaccgccgt atgatcggta cgatcgggac attgatatta tttacatact
      481 atatcattat tttttatacc cgtttttctg gtgaaacacc tgatgtctta gctttgaata
      541 cgagtttttt cattctatat ctgtgtgttg gcctgatcat cttggtttat tattggaaaa
      601 tctctaataa gaaattagct gatcagttat tagagcaaca agtcaggatg caacaggatt
      661 atatcaaaga tctagaaaaa aactatcaag acttgagaga attcaaacat gattaccaaa
      721 atctgctttt ttctcttcac agctatattt cagaaggcga cttagaagga ttacggtctt
      781 attatgatca aacgatatta cctacacgcc agatgatgaa cctttttccg gccaatctaa
      841 gtatgttgga taatatgaaa gttcctgaga tccgtagttt actgtcatta aaactcatga
      901 tggcacagga aaaagggttg atcgtccagc ttcattttcc tgagccaatc gaactggata
      961 cgaaacatac ggtaaacctt gtacgtatgc ttggtattat cttggacaat gcgattgaag
     1021 gagctagtgc tgcaaagaag aaaaaaatcg aattgaccat actcaagaag aatcattccg
     1081 tgatgattcg tgtgatcaat acaacaacaa atacattacc gttaaaccag ttgaaacaaa
     1141 aagggttctc aacaaaaaca aatccaaaaa acgaaggcct tggcttgttt atcttggatg
     1201 aattgacaaa gaccaatcct tatcttttcc ttgaaacatc aattgaaaac cagcgttttt
     1261 ctcaaacgat ttatatacaa ataaaataaa ggagtaatga tatgattccc atttatattt
     1321 gtgaagacga tccaaaacaa ttagaagttt tatcaacggt gatcaaaaat agaatcatga
     1381 ttgaaaactt agacagttat gtccatattt ctacctctga tccggaagaa cttctcaatg
     1441 cagccaagat cagaacttct tcttttggta tttacttttt agatatcgaa ctagaagaca
     1501 gtgaaatgga tggtgtagcg atcggtaaat tgatccgtga attagatcct ttaggaaaaa
     1561 taatctatgt gacttcccat acggaaatgt cgatgaaaat cttaaaaagc aacatcgaac
     1621 cgactgatta tatcgtcaaa gaagatcttt atgatttaaa agaaaatgtc gagcatatcc
     1681 tgagcaaaat tttcgaagat tttcaaatgt cccaaatcca aaaagatatc ttcaaaatag
     1741 agtttaatga tgagatcaaa tttttaccga tcaaagacat ccaatatttt tctacttcgc
     1801 taggagcgcc acataaactg gaagtccatt tgactcatgc ccaattacaa ttctatgaga
     1861 agatcaaaga aatcgagaaa atgcatcgga attttatccg ttgtcataaa tcctatgtta
     1921 tcaatacaca aaatgtgcgg tcgattaata aaaaaaccag agaagtcacg ttggcaaacg
     1981 gcgagaccat cccagcctct atccgtggat taaagaaatt gatttcataa aaagaaccga
     2041 atagtttgga ccgactaaat gaccagccga tccaaaccct tcggttc
//
LOCUS       DACDKL010000276         2067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDKL010000276 DACDKL010000000
VERSION     DACDKL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.281"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..609
                     /locus_tag="IV329_002700"
     CDS             <1..609
                     /locus_tag="IV329_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8941821.1"
                     /translation="LRDIDALKKDVSNFGQMFVEGNEPDSYGRNRRVFFISRDGFFLL
                     AMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLK
                     DTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYG
                     ELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDNE"
     gene            787..1128
                     /locus_tag="IV329_002701"
     CDS             787..1128
                     /locus_tag="IV329_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941822.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1121..1792
                     /locus_tag="IV329_002702"
     CDS             1121..1792
                     /locus_tag="IV329_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8941823.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1798..>2067
                     /locus_tag="IV329_002703"
     CDS             1798..>2067
                     /locus_tag="IV329_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941824.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
BASE COUNT          755 a          321 c          435 g          556 t
ORIGIN      
        1 ttaagagata ttgatgcact aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa
       61 ggtaatgaac cagattcata tggcagaaat cgacgagttt tcttcattag tagagatgga
      121 tttttcttgc tggctatggg gtttacagga aagaaagcta tctacttcaa acaaaaatac
      181 attgaagcat tcaacgaaat ggaagatgtt attcgcaaga atactgttcc tcaaacaatt
      241 gaagatatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg
      301 cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat
      361 atgaaagtta tgggagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa
      421 aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc
      481 gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttgccagaa
      541 actgcgatcc gtatggaaat tgatcaactg aacagacaac agagactttt tggtgatgac
      601 aatgaataga gctgaagcgc taagaatagg gacggcaatt gctaattgct ggtggaaata
      661 ctataaacca atcatcctaa gccaacaaca tattgacaag caaaaagcat ggcaacaaat
      721 aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa aacacatcat aaggagattt
      781 tagcatatgg aaaaagaact ttccactcta gatcaatatt tgattgatcc tgattggggc
      841 aagccgaaaa ttgaggaaac aagtggtcga aaaatcagac gaaatctttt gacgaatgaa
      901 gaactagctt gggatcaaga tgatttaggc aaccatgtaa ctatttggga tcatgtttat
      961 cttatccatc tatcgaagca ttcgaataaa cctgaatata tttacgtcat cgaagatggc
     1021 ttgattgatg cgctagaaga gtacgacaga gataacttga ttgatatctc ttattacgga
     1081 ccaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac
     1141 aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa
     1201 ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc
     1261 aacggtaact attaatgagt acaaacacta cagggttgtt tctggaactc atcaagactt
     1321 tcttgttgag gaatataaac cttttcttat ggatgaaact gggacttatg tatctgtctc
     1381 agtaacggtt aaaggacaca cggaaaccga attatttcct gttttagatt atcgaaacca
     1441 accagttgtt aaaccaaatg caatgcaaat caataactca ttgaagcgat gctttgtgaa
     1501 agcattggct ctacacggac tgggattata tgtatttcaa ggggaagata ttccaacacc
     1561 acctagaatc gatacaaaga aattaaacat gctagagacg attctagaag ctttcaatga
     1621 gcagatgggt aaagatatga ccaaaacctt aatcgaatat gttaatgagc agacagataa
     1681 attagggctc ttagctgata acgttgaaac tattgaacag ttaagctatg agcaatgtgc
     1741 cttgatggag cgagcaatag cagctaagag aaaagaatta gataagaagt gatatgagtg
     1801 tttaaaccat taatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata
     1861 agcgcaacca tcaatgagga agtgaacatt gatcgactaa agacgatgta tgacggctac
     1921 gatggtgatc gagtcattga aattcgtttt attgatccta gacgtttcac cgtacagcaa
     1981 cgaaacttca tctatgcgct gataggcgat atttttatcg atacaggcat gccaacggac
     2041 ttctggaagg aattcttcta cttccgt
//
LOCUS       DACDKL010000277         2065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDKL010000277 DACDKL010000000
VERSION     DACDKL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.282"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IV329_002704"
                     /pseudo
     CDS             1..492
                     /locus_tag="IV329_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IV329_002705"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IV329_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IV329_002706"
     CDS             1171..1617
                     /locus_tag="IV329_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941825.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IV329_002707"
     CDS             1619..1756
                     /locus_tag="IV329_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8941826.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="IV329_002708"
     CDS             1794..>2065
                     /locus_tag="IV329_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941827.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DACDKL010000278         2057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDKL010000278 DACDKL010000000
VERSION     DACDKL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.283"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IV329_002709"
     CDS             complement(<1..337)
                     /locus_tag="IV329_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8941828.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IV329_002710"
     CDS             complement(404..559)
                     /locus_tag="IV329_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8941829.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="IV329_002711"
     CDS             1176..1397
                     /locus_tag="IV329_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941830.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="IV329_002712"
     CDS             1398..1655
                     /locus_tag="IV329_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941831.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2057)
                     /locus_tag="IV329_002713"
     CDS             complement(1791..>2057)
                     /locus_tag="IV329_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8941832.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          603 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcact
//
LOCUS       DACDKL010000279         2056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDKL010000279 DACDKL010000000
VERSION     DACDKL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.284"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IV329_002714"
     CDS             complement(243..464)
                     /locus_tag="IV329_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941833.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IV329_002715"
     CDS             complement(562..807)
                     /locus_tag="IV329_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941834.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="IV329_002716"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="IV329_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          699 a          358 c          294 g          705 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catcca
//
LOCUS       DACDKL010000280         2050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDKL010000280 DACDKL010000000
VERSION     DACDKL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.285"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..268
                     /locus_tag="IV329_002717"
     CDS             <1..268
                     /locus_tag="IV329_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8941835.1"
                     /translation="KAIKELSKLLNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQED
                     IEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            281..913
                     /locus_tag="IV329_002718"
     CDS             281..913
                     /locus_tag="IV329_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8941836.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            1118..1345
                     /locus_tag="IV329_002719"
     CDS             1118..1345
                     /locus_tag="IV329_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP8941837.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            1414..1680
                     /locus_tag="IV329_002720"
     CDS             1414..1680
                     /locus_tag="IV329_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8941838.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            1670..1939
                     /locus_tag="IV329_002721"
     CDS             1670..1939
                     /locus_tag="IV329_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8941839.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT          732 a          302 c          429 g          587 t
ORIGIN      
        1 aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata atcagctgtt
       61 tttgttgtac attcactgta gttgtatgat tgaacgaatt gtacgtaaag aaagtgtgga
      121 tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg ataaaattca
      181 tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt tagagttgcg
      241 attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg gagtaatttt
      301 tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa gaatgaactt
      361 ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg tggggaaaac
      421 agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag aggaacttta
      481 tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag ctttacgaaa
      541 agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg cactggcatc
      601 ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta ctgaatattt
      661 ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg aaatatattt
      721 aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt caacgattgg
      781 tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac caatgcaaat
      841 cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac aaattgtttt
      901 agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc tattttgact
      961 aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca ccttttacga
     1021 aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg ttcgcaatcc
     1081 ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga accaaaatac
     1141 gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata ctgatactac
     1201 tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga tccctgaaaa
     1261 ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga gattttgcga
     1321 taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt ttgtactaac
     1381 gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat gaacggaggt
     1441 agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa agaactggta
     1501 ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa tgctgtactg
     1561 gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga agtttccaaa
     1621 gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa tgaattataa
     1681 agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa accaagctaa
     1741 aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc gtcagcacgg
     1801 aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa actatcgaat
     1861 attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg gacatagaag
     1921 tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag tcaatgtata
     1981 atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc ggttggtttc
     2041 tgctttttta
//
LOCUS       DACDKL010000281         2031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDKL010000281 DACDKL010000000
VERSION     DACDKL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.286"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..467
                     /locus_tag="IV329_002722"
     CDS             <1..467
                     /locus_tag="IV329_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941840.1"
                     /translation="CFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYL
                     MTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSS
                     FLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(543..878)
                     /locus_tag="IV329_002723"
     CDS             complement(543..878)
                     /locus_tag="IV329_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941841.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            1005..1220
                     /locus_tag="IV329_002724"
     CDS             1005..1220
                     /locus_tag="IV329_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941842.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            1257..1619
                     /locus_tag="IV329_002725"
     CDS             1257..1619
                     /locus_tag="IV329_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8941843.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            1665..1991
                     /locus_tag="IV329_002726"
     CDS             1665..1991
                     /locus_tag="IV329_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941844.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          713 a          286 c          421 g          611 t
ORIGIN      
        1 tatgttttaa cgaaaaagag gctaaaagag tatttcctca aaattcccga tcagaactca
       61 gcaaggaaaa acaattgatt ttagatgaag cacatgaagt tttacagggc ttatcgtggc
      121 aagggacata tctaatgaca gagaaagaac ttattaacac tgtttgtgaa cgcctacgta
      181 atgttgggga taacagcgtg aaaaatgctt taataaaaaa cagaggctac tttgttaaac
      241 aaggattcac tcttgaacga gcttctaagg atattaagaa actatacaat gtgaaagcaa
      301 gtagtagttt tttaatatat ttagcgaaag gggcagggat agatgaaaca ataaaagaat
      361 tgatattatc aggagctggg atagttcctc tgtatcaatt tacaagtgta acaagagatg
      421 atggacggag agtaaggaag agaaaagacc caattgtatt aaactagaca ggttatccat
      481 agctcttaat cttatttcat ctcaatacac ccaccgttaa agttcggtgg gttatttatt
      541 tttcaatctt tcaatagctg attgcgttca ttttcttgcc tttttaatag cttcatatag
      601 tcaattgttc gaactgcatc ttcagtgcta agagaacgat agagagccaa aaactcttcc
      661 tcataatcta cgacaacatt gtctagaaaa tggcttacag gggttcctag ggctctagaa
      721 agagctgtta agctatcgag gttaggctct gttacagaat tttcccatct tgctactgcc
      781 tgttgtgtag agaagatttt ttcagctaat tctttttgtg tccacccgag agcttttcgc
      841 ttttctctaa tgacctttgc atattgtttc tgtttcatta atacaccccc atctcagtat
      901 agtacaacaa gttgttgtat ttgtaatggt atttgaagtt ttttatattt tatgagttga
      961 aatacatcat attgttgtat ataataaaac aggaggggag aatcatgaaa aaagtaagta
     1021 atctcagccg atacagaaaa gaaaaaggac tttctcaaac ggagttagca aaaaaaatga
     1081 atgttacaca gcaatgtgta agttcgtggc aaacagggag aacgattcct aaaccctatc
     1141 aaatgaagat gctttctgag atattaagtg taccaatgaa tgagctgttt tctgatgtat
     1201 ttaataaagt aaacagttaa ctaataagca attgaatgat tttaggagga gaagacatgt
     1261 attgtagaaa atgtggcaaa gaaataacag atgaaagtaa gtattgccag ttttgtggag
     1321 cactacaaga aacagatgag acaaccggaa cagacacagt aaagaaaagt actgagcaga
     1381 caattaatga aatgaaggaa caaatcgtac atggacaaga gacagggaag ctgtatttga
     1441 ttattggttg gatttctatg gtagtatcgt tagcatttat tcctgttgta tttggtgctg
     1501 ttgctgttat tatgggctac ttatatcgag aaaaagatga gaaaatgggt acgattttga
     1561 tgatagcagg ggtagctgga gcaatctttg gtgtactttt agggatgtca atgtactgat
     1621 aagagggata agagcaaatt attttggaga taggagataa aaagttgaat aaagtaaata
     1681 tgaacaagaa gtttgtcatt ataggtattg ttgtcttggc tattataggt attatttttt
     1741 caaaaatgac aggtacatct ggattagaga agtcatcaaa accattggta acacagattt
     1801 tacaagaaca gtacaactta aatcgatcat gtgatgatgt gacgattact catgaaaatg
     1861 gagataacac ataccgggct aaagctatat tagacaatgg aagtgctatc aacataaata
     1921 ttgagtacta tccaaagaaa gaccgtatat atgtggagat accatatgct gaggtgttaa
     1981 tgttaaattg atttaaatgt attatgcata ccgtccaaac aggaactccc a
//
LOCUS       DACDKL010000282         2011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDKL010000282 DACDKL010000000
VERSION     DACDKL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.287"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IV329_002727"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IV329_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8941845.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IV329_002728"
     CDS             852..1040
                     /locus_tag="IV329_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8941846.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IV329_002729"
     CDS             1053..1610
                     /locus_tag="IV329_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8941847.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IV329_002730"
     CDS             1642..1884
                     /locus_tag="IV329_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8941848.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          734 a          309 c          407 g          561 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta a
//
LOCUS       DACDKL010000283         1945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDKL010000283 DACDKL010000000
VERSION     DACDKL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.289"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(77..160)
                     /locus_tag="IV329_002731"
     CDS             complement(77..160)
                     /locus_tag="IV329_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8941849.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            complement(181..292)
                     /locus_tag="IV329_002732"
                     /pseudo
     CDS             complement(181..292)
                     /locus_tag="IV329_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            complement(702..1496)
                     /locus_tag="IV329_002733"
                     /note="aph(3')-IIIa"
     CDS             complement(702..1496)
                     /locus_tag="IV329_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8941850.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1589..1840)
                     /locus_tag="IV329_002734"
                     /pseudo
     CDS             complement(1589..1840)
                     /locus_tag="IV329_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          587 a          431 c          304 g          623 t
ORIGIN      
        1 acgatttgtt tgtaatttta gttatctgtt taaaaagtca taagattagt cactggtagg
       61 aattaatcta acgtatttat ttatctgcgt aatcactgtt tttagtctgt ttcaaaacag
      121 tagatgtttt atctacataa cgcatttgga ataccaacat gacgaatccc tccttcttaa
      181 ttacaaattt ttagcatcta atttaacttc aattcctatt atacaaaatt ttaagataat
      241 gcactatcaa cacactctta agtttgcttc taagtcttat ttccataact ttagggttaa
      301 ccatacgcaa gaccaatcac tctcggacaa tactcatgat tttaatgata aaatccatgt
      361 taaacccata gataagaaat acacctgcaa taaccgttac ctgtttgtgc caatttaaaa
      421 atgcaccact tttttataat taaaacggtt gaaaactgta ccactaataa ctcacaatag
      481 agagatgtca ccgtcaagtt aaatgtacaa aataacagcg aaatttttaa atctatttct
      541 tatcgataca aattcctcgt aggcgctcgg gacccctatc tagcgaactt ttagaaaaga
      601 tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta gatgtctaaa
      661 aagcttgtag ttaaagcttt ttagacatct aaatctaggt actaaaacaa ttcatccagt
      721 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
      781 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
      841 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
      901 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
      961 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
     1021 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
     1081 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
     1141 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
     1201 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
     1261 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
     1321 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
     1381 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
     1441 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
     1501 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
     1561 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
     1621 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
     1681 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
     1741 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
     1801 gccccctgaa atccttacat acggcgatat cttctatata aaagataacc cgtggtgcta
     1861 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
     1921 atagtagtct caaaactccc aagaa
//
LOCUS       DACDKL010000284         1943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDKL010000284 DACDKL010000000
VERSION     DACDKL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.290"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(365..1529)
                     /locus_tag="IV329_002735"
                     /pseudo
     CDS             complement(365..1529)
                     /locus_tag="IV329_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          668 a          348 c          297 g          630 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ata
//
LOCUS       DACDKL010000285         1880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDKL010000285 DACDKL010000000
VERSION     DACDKL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.291"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IV329_002736"
     CDS             complement(21..995)
                     /locus_tag="IV329_002736"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8941851.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1638)
                     /locus_tag="IV329_002737"
     CDS             complement(1075..1638)
                     /locus_tag="IV329_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941852.1"
                     /translation="MEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVL
                     LAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKK
                     NGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGER
                     YYKVHDNWGDYHKVIPASWSNGTVSLP"
BASE COUNT          503 a          429 c          328 g          620 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt
//
LOCUS       DACDKL010000286         1839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDKL010000286 DACDKL010000000
VERSION     DACDKL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.292"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..340)
                     /locus_tag="IV329_002738"
     CDS             complement(<1..340)
                     /locus_tag="IV329_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8941853.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNF"
     gene            complement(318..632)
                     /locus_tag="IV329_002739"
     CDS             complement(318..632)
                     /locus_tag="IV329_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8941854.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(735..1016)
                     /locus_tag="IV329_002740"
     CDS             complement(735..1016)
                     /locus_tag="IV329_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941855.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(1021..1365)
                     /locus_tag="IV329_002741"
     CDS             complement(1021..1365)
                     /locus_tag="IV329_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8941856.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(1595..>1839)
                     /locus_tag="IV329_002742"
     CDS             complement(1595..>1839)
                     /locus_tag="IV329_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941857.1"
                     /translation="DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVA
                     KQNIKAPKKELSDNIDNALAELRYYVMQIKDVRITR"
BASE COUNT          515 a          396 c          231 g          697 t
ORIGIN      
        1 agaagttgtt ccaagaagca aaaccagttt taccattacc acgtcccatt aatgaaagat
       61 atctattgaa cactagtgtt ttatcttttt tccatcgaac accataaata aaacattgta
      121 gaaatttttc ccacggaatt aattcgaatg gaaagtattg tgctggtata ttgattgaat
      181 cctctaccat ctgcttatcg aagtaaatat cttctctagt aaagactctt tcttctagat
      241 aattttttag caataattgc tctttgcata ccttgatagt gccttcttct atagctttga
      301 accaattttc aatatgctta taactcagga attgattcat ttgcttcacc taccaattca
      361 ggagtaatgg caagtttatc caacatcaat cccatttgtt tgttgacaga aacaagcaac
      421 gctactgatt cattcttttt accattctcc agtctaatgc cgttctcgga tatatcttct
      481 tccagtgata tcgccgtttc ccataaactg atataacgat caacattatc taagaatggc
      541 tcaatatttg ttttctgact ttccaattgg cttattaaag agcggcgtaa tttttctctg
      601 tagcgatttt gagacaattc gtttttaaac attttagccc tcctttcatg ataaagttcg
      661 aaaaaatctc ttttcctgac agccccctcc gtttcatcac ccccaaaaaa tttgcgattt
      721 attttaaggg ggggttatct caccatcgga atgaaagctt cagcgaagtc aatgtaataa
      781 ttaatctctt caatactata tccaaaaaca ttttttattc tttcgacgtt attatcttta
      841 ttcaacgctt ctcttacttg attcactttg tatttactac aacagttatc tgataacaga
      901 tccctaatac ctacatagcg aacgtatatc aaacgtttaa ttaatccctg agtataagat
      961 gaatactctt caatcttttc tgtatcatac tctctgccat tatcattgat gatcatgcac
     1021 ttaccacctt tcacttgcat cgaagttagc aaagctttct atcttcttct cttgtttatc
     1081 caatgctgta agatatctgc catgaacttc attatgatgt tcaacacata aacaaataag
     1141 attatctaaa tctaaagcta agtcaggtct atccttgact tcctttatat gatgaacgtt
     1201 ctctactcta tgatacttac ctagtcttct acactcttgg cattcatagt gatctcgttt
     1261 catcgctttc tctctaagcc tgcgccattt aggagactga tagaacttaa ccaaacgatc
     1321 ttctcttatc aactgtaata gccatctata gaattcctcg ttcatgtccc gtctcctttc
     1381 acaatcttat ttaatgctta gctattcttt tgccatacaa tggaataact tcattgcttt
     1441 cctttcgttt atatgtatcg ctctttattg gtcttctata ctttcgtact atctctccgt
     1501 taccattttg cacagtgatt acttcatact tctgttctaa gtattgtggt ctatacattg
     1561 ttgttacctc ctttgtgcaa aataaaaaga ccactcaacg agtgatccta acatctttta
     1621 tttgcataac gtaatacctt aactcggcta atgcgttatc aatattgtca gatagttctt
     1681 ttttgggagc ttttatattt tgttttgcta cagtttccaa catcaatata gcatatccat
     1741 caaaaaagaa actattttca ttctttttct ccgttcgtac taattttgtt tcattttgaa
     1801 tcaatttcaa ctcatcatat acttctttcc actggtcat
//
LOCUS       DACDKL010000287         1779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDKL010000287 DACDKL010000000
VERSION     DACDKL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.293"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..583
                     /locus_tag="IV329_002743"
     CDS             50..583
                     /locus_tag="IV329_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8941858.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1218..1442
                     /locus_tag="IV329_002744"
     CDS             1218..1442
                     /locus_tag="IV329_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8941859.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            1462..1749
                     /locus_tag="IV329_002745"
     CDS             1462..1749
                     /locus_tag="IV329_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8941860.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT          715 a          249 c          318 g          497 t
ORIGIN      
        1 tatctgattt agataattta ttccagtagc aacctaggag gcaaccagta tgcaaccaca
       61 agaaaccttt acaataaagg aattgtcaga actttttaag atgagtaggc aagcaatatc
      121 gaaacatatc caaaaattag attcttccat gatagctaaa aatgagcgtg gttataaggt
      181 tgtattgcgt tctggagtat tgcaacttgc tcgcaactta ggcaaccaaa tgttgctaga
      241 taggttgcac gagtacgaac aagacaacga taaaaatgtt aatataccaa gcaaaaacgt
      301 ggttacaaac aagttgctag atcagttgcg tgaagaaaat gaatttttac gagaacaact
      361 tgccctcaga gagcaaaata attctcatat acaaaaacta ctcgaccagc aacaacaact
      421 caccctacaa gccaacaaac aaatcgaaaa actgcaagaa aaactacagt tgacttatac
      481 agaagaagct ccagagaatg actcaacgac tctttctgaa gcaacagaaa caatagacaa
      541 acaaattgat actgaaaaaa aatggtggca actttggaaa taactggagg gaacattcat
      601 gacaagaaaa aatcttctat tcggttctat tttactagga attctaggat tcagtccgat
      661 gttattgtca gtgatcaata aagggaataa acatggtgaa ttgaaattga atccaaatta
      721 ttacaacagt cctaacgaag aattgtttgt aaaagaaaca gataaataat aaaaaagact
      781 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      841 tatgaagcgc taccaaaaat ttatcatatt ttattaaaaa taacaaccca gtaaaaaatg
      901 actggtaaaa attcacaaag aaacggaaag aagcaaccgc agaaaatcaa gcttaaaaag
      961 agcttttgac ctagaccaat cgaaaatcac aaagtgagcg cctgtatcta ttgctacagg
     1021 cgctacttgg catagtgtac attatgtcta ctagctgttt aaataactga tcttataatt
     1081 ttatttaaac gattatgaat tcaaaactac tttttatgac gttaatcatt gttatataat
     1141 aaaatgttat ataataaaat tactaagatt atttaatata acaagcaatt ttaaaaaaac
     1201 gaaaaggaaa aacaatcatg aaaaagaaag tattaaaaca ttgtgttatt ctaggaatat
     1261 taggaacttg tctagctggc atcggtacag gaataaaagt tgatgcagct acttactatg
     1321 gaaatggtct ttattgtaac aaagaaaaat gttgggtaga ttggaatcaa gctaaaggag
     1381 aaattggaaa aattattgtt aatggttggg ttaatcatgg tccatgggca cctagaaggt
     1441 agtggtaggg aggttattat aatggatttt actaaagaag aaaaactttt aaatgcaatt
     1501 agtaaagtat acaatgaagc aactatagat gactatcctg acttaaaaga aaagctcttt
     1561 ctttattcta aagaaatcag tgagggaaaa agtgttggtg aagttagtat gaaattaagt
     1621 agttttcttg gaagatatat tttaaaacat aaatttggat tacctaaatc tttaatagaa
     1681 ttacaagaaa ttgttagtaa ggaatctcaa gtatatagag gatgggcttc tattggtatt
     1741 tggagttaat tctttggaat aaaatgaaaa tgggactga
//
LOCUS       DACDKL010000288         1725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDKL010000288 DACDKL010000000
VERSION     DACDKL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.294"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..1451
                     /locus_tag="IV329_002746"
     CDS             39..1451
                     /locus_tag="IV329_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8941861.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
BASE COUNT          643 a          285 c          321 g          476 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaatt
//
LOCUS       DACDKL010000289         1686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDKL010000289 DACDKL010000000
VERSION     DACDKL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.295"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            438..1568
                     /locus_tag="IV329_002747"
     CDS             438..1568
                     /locus_tag="IV329_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8941862.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          486 a          273 c          350 g          577 t
ORIGIN      
        1 caacttattt gttatttttt tctaaaaaat attaaagatt gttaaatttc actatttcat
       61 ttcaaattcc attgcctaat cattaggaaa agtctatcat gaactcgtaa atagaatata
      121 gtaataacct tgttaggtga ggctcctata caaacatagg ctgttgccca gaaacgtcga
      181 aagtcgccaa tgggtaaaac agaaactgtt ggattaaggc tttttccaat agctaagaat
      241 gttttcttta cgttgtatag tgctaaaact cggacgatga gatgatgcta ggtcagaatt
      301 cttgttcgaa aaggtttctg accttttctt tatatttttg tgactgactt atttgatgag
      361 acaaactcgc tcctcacctg acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa
      421 agatcgattg gtggattttg ggtccttatt tgactttgtc catgatcggt cttttagaag
      481 tatacagtgc cagttcttat cgtttattgc aagccgatga aaatacaaaa agtctattat
      541 tgcgtcaact aattttcata tttttgagtt ggagcgtgat cttcttagct cgttcagtca
      601 aactacacta tttacttcac cctaaaatag caggatacgg tttagcctta tcgattttct
      661 ttttagtatt agtaagaata gggatattcg gtgtcactgt caacggcgca caacgttgga
      721 tctctctgtt tggcattcaa ttccagcctt ctgaactggc aaatcttttt ttgatttttt
      781 atttaagctg gttttttcgt gacggaaata gtagcccaaa agatctaaaa aaaccattcc
      841 tgattacagt aggtataact tttctgattt tatttcagcc aaagattgct ggagcattga
      901 tgatcctttc gattgcgtgg gtcatatttt gggcagcggc ggttccattt aaaaaaggga
      961 tctatctaat cgttactttt tctgcattgc tgattggagc agcaggcggg gtattatatt
     1021 taggaaataa aggttggctt ccacagatgt ttaatcatgc ttatgaaagg atcgcgactt
     1081 taagagattc ttttatcgat agtcatggtg cgggctatca aatgacccat tcgttttatg
     1141 cactatataa tggtggcata tttgggcggg gattaggaaa tagtatcaca aaaaaagggt
     1201 atcttccaga gacagaaaca gattttattt tttcgattat tacagaagag cttgggctaa
     1261 tcggtgcatt gtgtgtgctt ttcttattgt tctcattatg catgagaatc ttttgtttaa
     1321 gcagtcgctg taaaaatcag caagctggat tgtttctttt aggattcgga actttgctat
     1381 tcgttcagac gataatgaac gtgggaagta tcgcggggct gatgccaatg actggtgtgc
     1441 cattgccatt tgttagctac ggaggaacaa gttatctgat cctttcttta ggaatcggaa
     1501 tcacgttgaa tatttcttca aagatacagg ccgaagagtt ggcgctctat cgacctgaaa
     1561 aacaataaag agaaactgag aaactctcag tttgaattat cataaagtac aatcttttat
     1621 ttttcaagcg agaggttctt ttagaaagag gaattcgcaa tgaaagtatc tcaaaaaatc
     1681 atatta
//
LOCUS       DACDKL010000290         1637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDKL010000290 DACDKL010000000
VERSION     DACDKL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.296"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IV329_002748"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IV329_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8941863.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          440 a          286 c          330 g          581 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtcta
//
LOCUS       DACDKL010000291         1616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDKL010000291 DACDKL010000000
VERSION     DACDKL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.298"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..421
                     /locus_tag="IV329_002749"
     CDS             <1..421
                     /locus_tag="IV329_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP8941864.1"
                     /translation="KIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPD
                     AVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIG
                     SWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            411..1553
                     /locus_tag="IV329_002750"
     CDS             411..1553
                     /locus_tag="IV329_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8941865.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
BASE COUNT          561 a          221 c          342 g          492 t
ORIGIN      
        1 aaagattata aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag
       61 tgctatagtg gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt
      121 aattaaccca gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga
      181 gcatgattgt agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg
      241 agtacatgta ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa
      301 aattggatct tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca
      361 ttccttagct tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta
      421 gaattttatt atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt
      481 ttaaagataa ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta
      541 caaaatatac tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac
      601 atcttgcctt aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta
      661 cttttatagc tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact
      721 cagaacctaa tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg
      781 aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg
      841 attatgaacg aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag
      901 cggaatcgtt aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga
      961 tctattcatt taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta
     1021 gtgataaaaa atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac
     1081 cgtattatca acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta
     1141 tcggacgtgg acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca
     1201 atcgatataa ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg
     1261 gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc
     1321 aagtagttaa cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga
     1381 aaccactaca tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc
     1441 ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga
     1501 cagatagtga acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat
     1561 attttatgaa cataggaatt attggtttag gttccatggg gaaaagaaga cttaga
//
LOCUS       DACDKL010000292         1611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDKL010000292 DACDKL010000000
VERSION     DACDKL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.299"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..1610
                     /locus_tag="IV329_002751"
     CDS             63..1610
                     /locus_tag="IV329_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8941866.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          544 a          290 c          333 g          444 t
ORIGIN      
        1 tcttgtgcgt ttcttgtggt attctgtaac aaaatcagtt cgattcaagg tggtgaaaaa
       61 ttttggaaac tacagatacg ttgcttcagt tgcttcaaga agcccataaa accaatcaag
      121 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
      181 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      241 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      301 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      361 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      421 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      481 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      541 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      601 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      661 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      721 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      781 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      841 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      901 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      961 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
     1021 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
     1081 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1141 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1201 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1261 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1321 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1381 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1441 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1501 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1561 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa a
//
LOCUS       DACDKL010000293         1610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDKL010000293 DACDKL010000000
VERSION     DACDKL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.300"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..532
                     /locus_tag="IV329_002752"
     CDS             134..532
                     /locus_tag="IV329_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8941867.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            516..1211
                     /locus_tag="IV329_002753"
     CDS             516..1211
                     /locus_tag="IV329_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8941868.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          610 a          231 c          320 g          449 t
ORIGIN      
        1 cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat gtgttattca
       61 ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg
      121 aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta tagaagcaga
      181 agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg aaacattaac
      241 agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt gtgatatgaa
      301 gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg tccaaactaa
      361 tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc cggaacttcc
      421 aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga caatcattaa
      481 aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt gatacagcaa
      541 atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta acaacaaatc
      601 ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa gaaatcaaaa
      661 aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa gaagaaatga
      721 tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc aaggtgccgg
      781 tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat ttgattaccg
      841 gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga gcagattata
      901 ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa gtgattgatt
      961 cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca gctagtttca
     1021 aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata acgttaggta
     1081 aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc
     1141 aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg
     1201 attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag
     1261 gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac ttatttctca
     1321 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1381 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1441 ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct tttttaaaat
     1501 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1561 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
//
LOCUS       DACDKL010000294         1610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDKL010000294 DACDKL010000000
VERSION     DACDKL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.301"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            225..461
                     /locus_tag="IV329_002754"
     CDS             225..461
                     /locus_tag="IV329_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8941869.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            480..695
                     /locus_tag="IV329_002755"
     CDS             480..695
                     /locus_tag="IV329_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8941870.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1398..>1610)
                     /locus_tag="IV329_002756"
     CDS             complement(1398..>1610)
                     /locus_tag="IV329_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAP8941871.1"
                     /translation="GQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLY
                     FDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT          542 a          246 c          298 g          524 t
ORIGIN      
        1 tagaaaaatt ctcaatatag ctttaataca gtgaaaatat tgtctcataa ttccacaaaa
       61 actttgaaaa cagttcttga acactaactt tttcattcct gaatcaaatt tattttggaa
      121 attttactat tcaagaataa acttgataag tttcaggaag atttttactg ttaatcaaat
      181 acgtatgtta cagtagcacc gtaaaaaata ttatttaaag aagcgtgttt aaacatgcaa
      241 aatgtaaagg aactaagtat tgtggaaatg cacaaactgt tggtggaggc aataatgctt
      301 ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg ggcagtaggt ggtgcaggtt
      361 taggaacagc aatttgcgga ccagcttgcg gtgttgttgg agggttatat ggagctgtcg
      421 ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata aaaaaggaga ataagtagta
      481 tgcatattaa aaacacaaaa acaacattta tattatcttc agaagagttg aaaaatattc
      541 aaggtgggag tgccgtggga gttttaggca ctactttttc tggggcaaca gcaggagtaa
      601 aactttgttc agccggagga ccttatgcaa tagctgcttg tggtgtaggt ggagctttat
      661 taggtgctgg attcagcatg tggaccggtg cgtaaacaat taaaaaatat caaacctcaa
      721 gattggtttg tgtcgattac gaacataggt atgttcttta tcttttcaaa agtttggaat
      781 ggacagaact ttttactaaa gcttcttgtg ctagttttta taattatatt aaatttttat
      841 cttttattaa agtcagataa aaattctttg gaaatgtaat ggtagagata tttttacaaa
      901 aggacttgca gcgctgtagt tggtgctgca ggaggaccta tttcggccgt tacaggaggt
      961 attggtaatg tacttgtcgg atgtgcatta aagcctgttc ctgcaagtta aatttataaa
     1021 aacaggaaag taattatctc aaatcctatc aagtacaatc ataggattgc acttgacagg
     1081 acaaattttt taactaaaaa atgaaaaaca ctttctaaaa attagtagaa tttgccaaat
     1141 ctttcgcatc ttccaccata catttaattt taattcccct ttatggtact taataaaaac
     1201 aagaaaagaa ctattcattt ggcccttttt tggttccaga ccatagaaat attcctccag
     1261 taactaatat tgctgttatg cacgtaacta ataacagcta taaaaattgg tacaacttta
     1321 ataggattca gaaaaatttg cactagtgaa ggaatctatt ttattagaaa aatgcaatct
     1381 ccctaaagaa aaaaccacta aacagtgctt tctttcaact tctccttaat tttttgtgct
     1441 ttccctaaag taaagtttct ggccaactct gaatcaaaat atagtgaacg ttttttaaaa
     1501 tctatttctt taacgttaaa gatatttact aaacacgctc gactaattct tgttaacata
     1561 ggatatttct tttcaagctc actaattctt ccataaaatt cgtactgccc
//
LOCUS       DACDKL010000295         1575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDKL010000295 DACDKL010000000
VERSION     DACDKL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.302"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..389)
                     /locus_tag="IV329_002757"
     CDS             complement(12..389)
                     /locus_tag="IV329_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941872.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(689..1570)
                     /gene="galU"
                     /locus_tag="IV329_002758"
     CDS             complement(689..1570)
                     /gene="galU"
                     /locus_tag="IV329_002758"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8941873.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          433 a          356 c          239 g          547 t
ORIGIN      
        1 cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta
       61 gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt catatatggg
      121 atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta
      181 cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa
      241 atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat
      301 agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc
      361 gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa
      421 aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat
      481 cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct
      541 attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa
      601 aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt
      661 tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact
      721 ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat
      781 ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg
      841 catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc
      901 ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc
      961 cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta
     1021 ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt
     1081 cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca
     1141 tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa
     1201 acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat
     1261 gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt
     1321 ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt
     1381 caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg
     1441 cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca
     1501 tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt
     1561 tgaccttcac ttgct
//
LOCUS       DACDKL010000296         1574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDKL010000296 DACDKL010000000
VERSION     DACDKL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.303"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..1532
                     /locus_tag="IV329_002759"
     CDS             54..1532
                     /locus_tag="IV329_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8941874.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          561 a          220 c          288 g          505 t
ORIGIN      
        1 ctttcatctg catatcaagt aattggtatg aggcattttt tgaggtgtag gatatgaagt
       61 tggattacct tatagaagag gaagatagag aaaaactgct gctagctaga tatttagaat
      121 ttgagaaaaa gaattatgta gtgctgccta agttatatga aacattaggt ttgtctaaat
      181 ttaaaacaag aaattatatc atagaattaa ataaagaatt tactgaattc aaaacaaagc
      241 cagagatcca aatttcagaa gaagcagaga tccatgtcga acgtctgaat ttgacggata
      301 tcaaaaattt aagaagaatc tactttcaac gatcaaaaat tgctcgactg ttaacggaga
      361 tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta agcatatcaa
      421 gagcctatgt aaaaagaaga gaattagtct cttttcttga acagcatcaa ataaagttaa
      481 agaaaaacat attagttggg gaagaaataa acatacggaa cgtattattt ggggtatatt
      541 tcggggtctt taatggtttt tctatgccat ttagagaaag cgtcatgaat gaagcaaaga
      601 gtatcgtgca atattttgaa tatctgttca atttaaaact gtctgcaaca aaacgaatca
      661 aattcaattt gctaaccact attagtttgt tgagggccta tgggaatcaa ccaatcggaa
      721 aacgtttctt ttcagatgaa tactttaaga gctcagaagg aatgaagatc atcaaggatt
      781 tatcacatat ttcttttctt catatcccta aagagcagat cattgatgag atttcttata
      841 tcatgttgtt tttgatcacg gaagaagaat ttgaattaga tggcgcattt ggacttgcgg
      901 aagctcattt tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa ttgattgatc
      961 aattaccatt ttcgattgaa atctctgaag aagaaagact atatatacga aaagaacttc
     1021 gtatgggtat gatgcatgtc aatcgaaaaa attggatatt cgactttgag atgactagct
     1081 ttacgtctaa aaagcagcta cagtttttct tcgagacgta tccaatattc agtacagcaa
     1141 tccaaaaggt catacaagag aataaacgct atttcttggg aaaagatgaa cgtttcatga
     1201 ttcgcttatt ttatgattat ctctttttat tggtcggtat ttgtccggta tcatattttg
     1261 aagcaccgat tcatgtctgt attgattttt cgcaaggatc tgcttatacc gattacatta
     1321 tttctcaagt gaaaggattt aaaaattaca atattgtgat tgagagtcga atcacttctc
     1381 gtacgaatat ttatttatct gattgtttac ttgaaaaatt taataaagaa cagataattt
     1441 ggaaaaatcc accgactcca gatgactggg aatattttgg aaataccatc atcagagaaa
     1501 aaaatagaca tttaactgaa aaaatcagct agtatttatg gatggcagct ttgtctcaca
     1561 tatttattag ctga
//
LOCUS       DACDKL010000297         1570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDKL010000297 DACDKL010000000
VERSION     DACDKL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.304"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..344)
                     /locus_tag="IV329_002760"
     CDS             complement(<1..344)
                     /locus_tag="IV329_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8941875.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
     gene            complement(640..1512)
                     /locus_tag="IV329_002761"
     CDS             complement(640..1512)
                     /locus_tag="IV329_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8941876.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          425 a          359 c          306 g          480 t
ORIGIN      
        1 cccgtcttaa accggcgata gagggtactt actgaacaag agaaggttcg ctctgcacgg
       61 ccaataatga cgtctggggt ccaaccttca gttgatcttt ttgcaatgta ttctttttcc
      121 tcagcaggaa aaatgatttc ggttctccca caacgtcgct tattttcttt gtattgttca
      181 aagcattcaa gtgctgttcc accacatttg agaaagttat agacattgta aattgtttgg
      241 cgtccacgtt taagctgctt cgcaacgata gcaaccggag tttcttgatg aaaatatgct
      301 tctatcatta caagctcgtt tggtgtaaga tgggtataag tcatttatgt tcactctcct
      361 tgtatgcttt agcgggtatt acgatttgag tgtaacataa atggcttttt tatttgtctc
      421 gcttaattat acaaacggcg tgtaaaaaac tattgcacaa tacaagtggc ctacggctat
      481 cgaaatttcc ataactttcg agcaagaatt tatctcattc aaggcttgat cttccaagta
      541 aaacaaaaac cagttaagca ctctgcctaa ctggttatga ctaaattttc agttgtatct
      601 ttctctacca acactaaatg tcgaagagcc ttttttcgtt tatgaatgta agaccgcttc
      661 tcgagccaat gccaaacaac cgagcgttcc agcgttgtct tctaatgctg gggtcacgat
      721 atatttgctt aactcaggtg tttcgacata cccattgacc aattttgcaa atgcgtcgtg
      781 gacgttttgc agcatatggc gttgtttcat cacaccaccg ccaaaaatga tcacatctgg
      841 agaaaacatc aaggttgtat tgtaggcaca ttgagcaata taggaggctt cgatttccca
      901 ataaggatca tcttctgcaa cctcttgtcc ttttttccct aagcgttttt cgatggcggg
      961 tccagcggcc atcccttcta aacagttgcc atgaaatgga cagctgccag caaagtcatc
     1021 ttttggatga gggacgacga gcatatgccc catttctgga tgactaaacc cttcgataaa
     1081 gcgcccttct tgtaaggcac cgccgccaac acccgtaccg atcgtatagt aaacgacact
     1141 gctcaatcct ttgccactgc cgaagacata ctcaccatag gcagccgcat tgacatctgt
     1201 tgtccaagaa atcgggatcg gaaattcttt tttcatggta ccgatgaaat caaaattttg
     1261 ccatgccaat ttcggggtcg aggtaatgta cccataagtg gcggaatcgc gatggatatc
     1321 gataggccca aatgagccaa tcccaatccc tgctaaatct gcttggtatt gtttgaagaa
     1381 gtcaatgact aaaggcattg tttcctcagg tgtcgttgtc ggaaagctca ctctttcctt
     1441 gatcgtcatt tcttcatcgc caatggcaca cacaaatttt gtgccgcctg cttcaattgc
     1501 accgtatagc atagacattt ctccctttat tttagaatga aatcactaaa taaatgacta
     1561 tatatcatat
//
LOCUS       DACDKL010000298         1560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDKL010000298 DACDKL010000000
VERSION     DACDKL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.305"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..460
                     /locus_tag="IV329_002762"
     CDS             38..460
                     /locus_tag="IV329_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941877.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            470..673
                     /locus_tag="IV329_002763"
     CDS             470..673
                     /locus_tag="IV329_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941878.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            884..1468
                     /locus_tag="IV329_002764"
     CDS             884..1468
                     /locus_tag="IV329_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941879.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          550 a          224 c          291 g          495 t
ORIGIN      
        1 aaacgttttc ttagttttga tttcgaggtg atagacaatg aaaaataagt ttattttatc
       61 tttaggtatt ttaactattg gaattagtgt aactggaact acaattccta catttgctga
      121 tagtacaaat gaaccaacta ctgtagtaga taataaccaa gaattaactc ctttagatag
      181 ggacggaata gccattggta acccaactaa cgacttaact gttactgaaa attatttaat
      241 agtttctcca ttacttgctc tagcagcttc taaaacgata cgagtgacag gaacgatgat
      301 ttttgataaa gttggttggc cttctagacc tcctcaatca cggaattgga aagaaaaaag
      361 aaatggttat tggtactatg gaaaattgta tttacaaagt tataaatcag ctggaaacaa
      421 ttggattgcg acttattcag gaaccttaac tagaaagtag ggatttttta tgccagaatt
      481 tgtgaacaca ccaaattttt atcaagatag cgatattcta agtaaaatta atttaccgaa
      541 agaagaatca gctttacaag ttcagcgaac tttacaggag acacaaaaaa ttcaaaaatc
      601 aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa agagaccaaa aaacatatca
      661 aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg acattctttt tttctaattg
      721 aaagtgttca taaaatcacc catttctgga cactttcttt ttttgcttta tttttcaaaa
      781 aaacaattca tttcatgttc ataaacccat aacaatatct atgttcagtt aaactatatg
      841 gataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc
      901 ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg
      961 ttgtgaaaaa atttttactg atcatatgag tggggcaaaa agtaatcgac caggattaga
     1021 aatggccatt gattttgttc gctctggcga tacacttgtc gtttggcgct tagatcgttt
     1081 aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag
     1141 ttttcatagt attcaagaaa atattacgat ggacaaatca agttctacag gacagctcat
     1201 gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc
     1261 agcaggacga gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc
     1321 tgaacaagat ttggaattac tgaagacttt agtggatagc ggtacaccta ttaagactat
     1381 tgcggaacgg tggaacgtct caagaaccac catttatcgt tatttggcaa aaatggagga
     1441 gaaaaatgga aagacaaatt ctcattagtt tttgtcattt aatgctgaaa atttttgcca
     1501 tttatctaga aaagtgacaa tagtatgaga attttttgta agtgtctact gataacttat
//
LOCUS       DACDKL010000299         1555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDKL010000299 DACDKL010000000
VERSION     DACDKL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.306"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IV329_002765"
     CDS             complement(<1..1097)
                     /locus_tag="IV329_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8941880.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>1555)
                     /locus_tag="IV329_002766"
     CDS             complement(1227..>1555)
                     /locus_tag="IV329_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8941881.1"
                     /translation="MVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSA
                     SSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKE
                     LLIDNN"
BASE COUNT          479 a          317 c          262 g          497 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catct
//
LOCUS       DACDKL010000300         1554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDKL010000300 DACDKL010000000
VERSION     DACDKL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.307"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(35..106)
                     /locus_tag="IV329_002767"
     tRNA            complement(35..106)
                     /locus_tag="IV329_002767"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Gln,seq:ttg)
     gene            complement(116..188)
                     /locus_tag="IV329_002768"
     tRNA            complement(116..188)
                     /locus_tag="IV329_002768"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:His,seq:gtg)
     gene            complement(250..334)
                     /locus_tag="IV329_002769"
     tRNA            complement(250..334)
                     /locus_tag="IV329_002769"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Leu,seq:caa)
     gene            complement(355..425)
                     /locus_tag="IV329_002770"
     tRNA            complement(355..425)
                     /locus_tag="IV329_002770"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Cys,seq:gca)
     gene            complement(471..542)
                     /locus_tag="IV329_002771"
     tRNA            complement(471..542)
                     /locus_tag="IV329_002771"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(508..510),aa:Gln,seq:ttg)
     gene            complement(554..626)
                     /locus_tag="IV329_002772"
     tRNA            complement(554..626)
                     /locus_tag="IV329_002772"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(591..593),aa:His,seq:gtg)
     gene            complement(629..702)
                     /locus_tag="IV329_002773"
     tRNA            complement(629..702)
                     /locus_tag="IV329_002773"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(668..670),aa:Trp,seq:cca)
     gene            complement(709..789)
                     /locus_tag="IV329_002774"
     tRNA            complement(709..789)
                     /locus_tag="IV329_002774"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:Tyr,seq:gta)
     gene            complement(791..866)
                     /locus_tag="IV329_002775"
     tRNA            complement(791..866)
                     /locus_tag="IV329_002775"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(831..833),aa:Phe,seq:gaa)
     gene            complement(902..974)
                     /locus_tag="IV329_002776"
     tRNA            complement(902..974)
                     /locus_tag="IV329_002776"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Asp,seq:gtc)
     gene            complement(984..1056)
                     /locus_tag="IV329_002777"
     tRNA            complement(984..1056)
                     /locus_tag="IV329_002777"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Val,seq:tac)
     gene            complement(1072..1143)
                     /locus_tag="IV329_002778"
     tRNA            complement(1072..1143)
                     /locus_tag="IV329_002778"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1108..1110),aa:Glu,seq:ttc)
     gene            complement(1158..1247)
                     /locus_tag="IV329_002779"
     tRNA            complement(1158..1247)
                     /locus_tag="IV329_002779"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1211..1213),aa:Ser,seq:gga)
     gene            complement(1287..1360)
                     /locus_tag="IV329_002780"
     tRNA            complement(1287..1360)
                     /locus_tag="IV329_002780"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1324..1326),aa:Asn,seq:gtt)
     gene            complement(1370..1485)
                     /gene="rrf"
                     /locus_tag="IV329_002781"
     rRNA            complement(1370..1485)
                     /gene="rrf"
                     /locus_tag="IV329_002781"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          437 a          406 c          347 g          364 t
ORIGIN      
        1 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
       61 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
      121 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
      181 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
      241 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
      301 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
      361 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
      421 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
      481 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
      541 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
      601 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
      661 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
      721 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
      781 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
      841 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
      901 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
      961 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
     1021 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
     1081 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
     1141 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
     1201 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
     1261 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
     1321 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
     1381 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
     1441 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
     1501 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt tttt
//
LOCUS       DACDKL010000301         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDKL010000301 DACDKL010000000
VERSION     DACDKL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.308"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            20..1486
                     /locus_tag="IV329_002782"
     CDS             20..1486
                     /locus_tag="IV329_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8941882.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          421 a          289 c          325 g          495 t
ORIGIN      
        1 aagaattggg gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa
       61 gggattgtca actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg
      121 tgcattactt atctattata tgtacgatac agttgccaac gggggacttg gattaccacg
      181 agcaacagca ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt
      241 cggcggatgg gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt
      301 tttcattatg cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt
      361 ttcgatggca ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt
      421 cggacatctt tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat
      481 gggtgtcaac ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata
      541 taactatcat ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata
      601 catcatccaa ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac
      661 aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc
      721 agtattcggc ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat
      781 cagtgtattg gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga
      841 tgtaactgct gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat
      901 cattttctgg tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg
      961 tacacaatct acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc
     1021 aattttcatc tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg
     1081 tcaaccatct acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt
     1141 gctattgatg ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt
     1201 gttgggaagt ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc
     1261 tgtgacaaca aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt
     1321 agctgatgct tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac
     1381 agaatctgct tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt
     1441 tactgtgaaa gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa
     1501 aacaggaatt gcttatttgc agcagttcct
//
LOCUS       DACDKL010000302         1527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDKL010000302 DACDKL010000000
VERSION     DACDKL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.309"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(368..1171)
                     /locus_tag="IV329_002783"
     CDS             complement(368..1171)
                     /locus_tag="IV329_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8941883.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          464 a          304 c          188 g          571 t
ORIGIN      
        1 aaaaaactta tctaattact ctatattaac ttttaacaaa tagttgactt gtccaaaaat
       61 gttaccttca atctcacaaa gatattaaga tgtttcctac caaaactgat atcaccggac
      121 tttttattct gtgttgtcca aaagtgttac ctttagtcga tcaacaaatt aaaaaaaatt
      181 gtttttttct tgacttgtcc aaaagtgtta cccatagttg ttaaaatttg tatagattag
      241 ccattctttc gaatattatt tagactgaaa cttgcttaca aaaaaacttc ttgcatttat
      301 tgtccaaaag tgttacccat acttttttaa agaagcaggt tgtattaaaa ttacaacctg
      361 cttcttctca atcttcttct aaccaattat gcattgaaat ggttggcaag tttgattctg
      421 gctcactaaa agtgaatctg agccgatcaa ctcttcttcc tttctttgca tagatttttt
      481 cataatcaaa agtttcaaat acctgtattc cgttttcatc aggagctaaa aattcttctt
      541 tcgcctgctc tagtattctt ttgtcaatat ttgtcattct ataacttgaa ggtatactta
      601 atgctcttct aaaatcttca actttgacta cccaataacc atttcgccac tctttatttc
      661 tgtattgcat gagatgacga tacaattctt ttgtgtagct acttcttagt tttgtaaaat
      721 tatcaagttc aaaacgagta aagtttttgc caattgagtt taagataaat tcaaattcct
      781 catttgcctt aattgtaact gtttccttct gtttatctat ccggaatttt tgaaaaagcc
      841 acagctgttc atagacgtct gaatcttcaa aacggtaggt taaagtacct agtttgtcac
      901 tcaaagcttt taacacagaa taaaaactgt ccttttctct tctgtcgtat ttgaccaaat
      961 aacgaaagag gttgaaatca aaagttactt ctgctgttcc ttgacgtttc atttttgata
     1021 aaatagccca aaacacatcc atttcaacag gcttaaatcc tcgcaaagct gttgtattca
     1081 gaccattgtt ataacgcaca attgcatttg tttgttctga tactaaatct acatcactat
     1141 caataatctt ttcggtaact tcttgagaca ttgattactc tccaatcttc tttttgcgct
     1201 cctttaatca tgttaatctg gtactaatta aaggaaacaa ttttttctgc ttgttaccta
     1261 aattaaatca caactaaagg taacagtcaa caaagatttg ttactaatct aattttctat
     1321 tttttcgcct tccaaatcaa gaaaaccgaa ggtaacgtct tatcctagtg tgatacctca
     1381 aatgtccact tttgttacct ttgctgtcca aaagtgttac ccttactgtc cacttttgtt
     1441 acctttactg tccaaaagtg ttacctttat acccctttaa tacttactct cccaagagat
     1501 taagcctccc ctaacagata tattaac
//
LOCUS       DACDKL010000303         1517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDKL010000303 DACDKL010000000
VERSION     DACDKL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.310"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV329_002784"
     CDS             131..505
                     /locus_tag="IV329_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8941884.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV329_002785"
     CDS             complement(619..1248)
                     /locus_tag="IV329_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8941885.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV329_002786"
     tRNA            complement(1382..1454)
                     /locus_tag="IV329_002786"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          513 a          229 c          265 g          510 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcg
//
LOCUS       DACDKL010000304         1476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDKL010000304 DACDKL010000000
VERSION     DACDKL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.311"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..794)
                     /locus_tag="IV329_002787"
     CDS             complement(<1..794)
                     /locus_tag="IV329_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8941886.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRR"
     gene            938..>1476
                     /locus_tag="IV329_002788"
     CDS             938..>1476
                     /locus_tag="IV329_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8941887.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSS"
BASE COUNT          466 a          242 c          281 g          487 t
ORIGIN      
        1 cgtctaagca agccgtttga gtgctcattt agccctcttt gcgaaggtgt tcctggatct
       61 gcgaaaaaga tggcaatatc gttcgagtta cttatagatc gccagttgga aaattctttt
      121 ccacaatcaa atgtcatgga tttaaacaag tgtttaggca tggatttgaa ccaatcattt
      181 aacctgtttt cgatatcttc ggctcgtctg cctgtcggtt tcaaagtaat aataaccttg
      241 gataaacact caaccaatgt aataacagcg ctcttgtgtt taactcccac aatggtgtcg
      301 acttcgagat gaccaaattc tttagtcagg tgtggataga gactgttccg ttcatgaatc
      361 gttctgcgaa aggcctgttt tccacgcttt tcttgatgat tattcggttt acgtttcccc
      421 ttcataggta acgactgtgt gtcaaaaagg ccttttttaa acatccgata gagtgtacga
      481 acagaacaat caattggaaa ctcgccacgg ccgataatca catcgggtga ccatccttga
      541 gctacctttt cttgaacata tgttgattgt tcatcagata aaacggttga acgtcgtcca
      601 caacgagatt tattttgttt gtattgacgg taataatcta gcactttttt tccttgtttg
      661 agaaacgaat aaacattata aatggtttgt ctagagcgat ttaaactttg tgcgacaaga
      721 gcgacaggtt tctcgatatt gaaataagac tctatgatta ctagttcgtc tgttgtaaga
      781 tgggtgtaag tcatttgtac tcactcctta ttaattcttt ggtcggaact attttgagtg
      841 tagcacaaat gatcttttta gttgtctagc ttaattttac aatcgacgtt ttaataaatt
      901 gtgatataat gaagtataac tttttaggga gtgtactatg aattttcaag ataatagacc
      961 attatattta caagtagaag cttctatacg taacgatatt cgtgaaaaaa aatatcttcc
     1021 aggagagcaa ttacctacag aagaagaatt atgtaaaatt tatggagtaa gtaagattac
     1081 cattcgaaaa gcttttaaac ttttagcaga aagtggaaca gttgaacgat taagggggaa
     1141 agggaccttt gtaactcaaa aaaaagaaag tttaaaatta gctggagttc atggatttag
     1201 cgattctctt tctacccgag gacataaaat tagatatact gttttacatt ctaaaattat
     1261 aaaggctgat gagtttttag ctaataaatt acaaatcaat gtaactgata gagtttacaa
     1321 tattaaacgt ttaatgtggg aagacggtgc cccgatggga attgataatt ttttcgcatc
     1381 agaagaaaaa tttccaactt tatttgaaaa aggtagccaa tatacttcat tatataaact
     1441 tctagaagat gactatgatg tcaaagctac gagctc
//
LOCUS       DACDKL010000305         1464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDKL010000305 DACDKL010000000
VERSION     DACDKL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.312"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(576..941)
                     /locus_tag="IV329_002789"
     CDS             complement(576..941)
                     /locus_tag="IV329_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941888.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1017..1262)
                     /locus_tag="IV329_002790"
     CDS             complement(1017..1262)
                     /locus_tag="IV329_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941889.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          514 a          250 c          269 g          431 t
ORIGIN      
        1 atttagaaat tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt attttagtct
       61 attttcaggg ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag
      121 caatggcagg ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag
      181 ccacgacaga ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac
      241 gatgcatggg aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt
      301 gtagaagaag caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat
      361 cgtatacaga cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt
      421 ctagggaagt tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg
      481 aagataaccc taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat
      541 gaagtaagag gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc
      601 tccaaatttg ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg
      661 ttgcgctcta cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga
      721 tttttccact ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga
      781 tgatattgga ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc
      841 tttccccagc catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga
      901 cgaggaagct ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc tcctttatat
      961 atagattgtt aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac
     1021 ttcagtttgc ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag
     1081 tcagcgatat agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga
     1141 tatttgaata aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca
     1201 gttcttaaga aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc
     1261 atttaatcac ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca
     1321 atcaatagta ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa
     1381 gacaaaacca gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa
     1441 ttaaaaactt tccacataaa gtag
//
LOCUS       DACDKL010000306         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.313, whole genome shotgun sequence.
ACCESSION   DACDKL010000306 DACDKL010000000
VERSION     DACDKL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.313"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IV329_002791"
     CDS             105..1406
                     /locus_tag="IV329_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8941890.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          288 c          264 g          412 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttct
//
LOCUS       DACDKL010000307         1419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDKL010000307 DACDKL010000000
VERSION     DACDKL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.314"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1220)
                     /locus_tag="IV329_002792"
     CDS             complement(<1..1220)
                     /locus_tag="IV329_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941891.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLA"
     gene            complement(1318..>1419)
                     /locus_tag="IV329_002793"
     CDS             complement(1318..>1419)
                     /locus_tag="IV329_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941892.1"
                     /translation="KYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT          451 a          274 c          165 g          529 t
ORIGIN      
        1 gctaattgtt cccccgacaa aatacaagtc acactttcct tactatcatc aaaaaaatta
       61 acatcagaaa gcaagacatc atagacacgt cgcatttcct ctagataatt ccttattgtc
      121 ctgaaaagtt tgtctttcat tgattcaaga gtgtcaaaaa gtacttttga ttcctctttc
      181 aattttcgaa aatgtgtagg atacaagtct aggttctcta atagccaatt aaatgtttct
      241 acccgactgt ctttctttaa aattagcatc acaactccaa taaaattttc actgatatca
      301 caattactgg aatgacaata aaaaatggaa tgcccagttt tactggtaaa aatactagca
      361 gagggattat gatcgttgtg aaacagacat cttaagtttt tacttgactt aagtaattta
      421 ttcatatcaa tggttaacaa tctttcataa atctcattaa aagacatgtc ggaggaatca
      481 aacaggcagt ctttaaacca ctgtttcatt tcttctttgt cgtcattttt tatcaactta
      541 acaaaatcgg tttcattgac tagttcatac tctctccctg aatcactttg acaattttta
      601 gaacatgtca tggtaaaatc aatcggaagc tcagtaatat ctaacatgtt gtcttcatta
      661 attattatca ctgcatctga cttatatcct ccaaaaaaca ttctattcga agcatttaca
      721 ttgtcatcac acccggggat gttctttttc aagtattcgt aaacagcaac cattttacca
      781 tagtcagtaa ttgcttcttt taatagaaag acaatcttgt atttttctag attttcagag
      841 taccgaatac ttttttgtat cgcaaacgca gaattatgaa ctaaattgat agataaaacc
      901 ttttctacgc ttaagtatct actatcatct ttactcaaat ctactacctt ccccttctca
      961 tctttaacag tgttatcgac atccagaata attactctgg tttccacaaa actctccttg
     1021 tcattgcctt tattctttgt cttgcttgga ataaatgatt gtgatgaatt tagtatctct
     1081 acaaactcat ctcttgtaac ttctctatag ttatctaaac acctatctct tacatcagtt
     1141 ttttcttttt ttgtaactgg cttctctaat aatttttctt gatctacata aattggaata
     1201 ttaatcattg atgtttccat ttgtgtttcc tccttataaa attctgttaa agactaaaac
     1261 atacagataa taaaaacaaa ctctttatta ctatatgttt tagtcttatt tgtttactca
     1321 acctttatac cgttccatga aactatcaat atcagatttt ttaaatctaa tgaccgtatc
     1381 aatttttata acaggtaacc cgtattgaga aacatattt
//
LOCUS       DACDKL010000308         1402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDKL010000308 DACDKL010000000
VERSION     DACDKL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.315"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV329_002794"
     CDS             <1..1350
                     /locus_tag="IV329_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8941893.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          557 a          223 c          304 g          318 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ct
//
LOCUS       DACDKL010000309         1386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDKL010000309 DACDKL010000000
VERSION     DACDKL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.316"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..801
                     /locus_tag="IV329_002795"
     CDS             <1..801
                     /locus_tag="IV329_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8941894.1"
                     /translation="TEKIKIYQLVTKNEKKLFLNTTFENLTLINPNIMNFEDESIIEA
                     SGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQ
                     KEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMS
                     IVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIK
                     KYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            817..>1386
                     /locus_tag="IV329_002796"
     CDS             817..>1386
                     /locus_tag="IV329_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8941895.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCE"
BASE COUNT          495 a          228 c          250 g          413 t
ORIGIN      
        1 actgaaaaaa tcaaaatcta tcaattagtt actaaaaatg aaaaaaaatt gtttttgaat
       61 acaacatttg aaaacttgac attgatcaat ccaaatatta tgaatttcga agatgaaagt
      121 attattgaag catctggatt tagaatccaa cctgaatatg aactattcaa acgtttgttt
      181 gatatcatcg tttcacttgt tttattagtt attgcttcac catttatgtt attcacagca
      241 attttagtca aagtgacatc accgggacca atcatctata aacaagtgcg gatcacaaaa
      301 aatcaaaagg aattttcaat ctataaattc cgctcgatga ccgccacagc cgaagcaaaa
      361 tctggaccag ttttagctaa gagcaatgat gcacgagtaa cgcctgtagg taaatttatt
      421 cgagctgtcc gctttgatga attaccacaa atcttcaatg tgctgaaagg cgatatgtcg
      481 atcgtaggac cacgtcctga acgttctttc ttcgtcgatc agttcaatgc agaaaaccct
      541 tattactact tacggcataa tgttcgtgct ggtatcactg ggtatgcaca agtctatgga
      601 aaatatgtct ctgattacaa cagtaaatta cgttttgatt tactttatat caaaaaatat
      661 tcattgatgc ttgatttaaa gatcttgctg caaaccatca agatcctatt tgataaaatg
      721 tcctctcaag gacttgaaga agacgaaaat gtgatctcat tggcagaaat tgcttttccg
      781 aatgaaaaaa tctttcgcta gttgggaggc gtaaaaatga acaatccgaa attcagcata
      841 atcgtaccag tatacaatgc agaagcaact attggtcgaa cgattgatat tctaaaagaa
      901 ttggattatg aagagtacga agtcgtttta gtaaatgatg gatcaacaga tcaaacacag
      961 gaaatcattc aagaactgat tgaaaatgat gaacgttttg aattgattac tacgaccaat
     1021 caagggcccg gattagcaag aaacgaagga ataagacagt caaaaggaga atatctctta
     1081 ttcttcgatg cagatgacac gccagtaaaa acgatcttgt cagattatga tcatttgcta
     1141 gaaaagagtc caaaagcgga tcttgttgtt agttcgtttg tttttcgtac aatggacgag
     1201 ggaagaatcg tttccgaaaa agaaaactta gttacagaat gtcactatct aactcacgaa
     1261 gagtttataa aagacatgta tcagttaatg aatgaacagt tgatgtatgt tgtatggaac
     1321 aaatgctatc gtagcgatat tctgaaaaat aaccaaattc tatttaaaac ttataatagt
     1381 tgtgag
//
LOCUS       DACDKL010000310         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.317, whole genome shotgun sequence.
ACCESSION   DACDKL010000310 DACDKL010000000
VERSION     DACDKL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.317"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IV329_002797"
     CDS             191..445
                     /locus_tag="IV329_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8941896.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IV329_002798"
     CDS             446..763
                     /locus_tag="IV329_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8941897.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DACDKL010000311         1370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDKL010000311 DACDKL010000000
VERSION     DACDKL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.318"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            219..632
                     /locus_tag="IV329_002799"
     CDS             219..632
                     /locus_tag="IV329_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8941898.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            769..1029
                     /locus_tag="IV329_002800"
     CDS             769..1029
                     /locus_tag="IV329_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941899.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            1147..>1370
                     /locus_tag="IV329_002801"
     CDS             1147..>1370
                     /locus_tag="IV329_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941900.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFS"
BASE COUNT          438 a          200 c          226 g          506 t
ORIGIN      
        1 tttttctata tttggataga agaatttact tttttacctt ttgacatatt ttcttcttat
       61 tattttaatg atcccttttt caaattagaa agatagcctt tgtatcgtat tagatgaaat
      121 attcacaaag ctaaccatta tacttgcatt tttttcaaaa tagagttatt acgtaaatag
      181 ggataaataa aaaatactta ttgaaaggat gatcaaaaat ggcaaatgta cctagcattc
      241 gcgacatgtt ccctgatttc aatgacgtgt ttagtccagc attcaatgat tttttaggtg
      301 tttcaagtta tccaaaagta gatttagtcg aaaatgaaaa agaatacaaa ctaactgctg
      361 atatgcctgg ctgtgacaaa gaagacacga cagttgaata ttcagataat actttgacga
      421 tctccgccaa tcatgagtcc catacagaag ataaagaaga tggcaactat gtacggaaag
      481 aaagacattc ggtctcttac aaacgttcat tctatcttcc taatgttgat gaagaaaaaa
      541 tcactggaac ctttaaaaac ggtgtgctga aattggtatt acctaaaaca gcttatcagc
      601 caaaagaaac aaaaaaaatt gagttaaatt agaacttatc gcctctcgat gaatgagggg
      661 cgatttttat atatttggat aagaacgagt atgtttaaat tttcaaaatg agcaaaaaaa
      721 tattttgttt ttacgataaa gatgttatga tctctcttgg taaaaaatat gggagttgat
      781 catttgattg tcttaagtaa acttttgttt tggataccat ttatcataat gaccagtgct
      841 ttcttttact tattcaaaag gcctaacaaa tacttactct tatctgcttg tccgatgatc
      901 tattttatcc ataagattag aggattttat tattacgact ctggtatctc attgttttgc
      961 tacgaaatcg gcttattagc ttcggccatc tttttcatat ttgtgttaga atatttcaga
     1021 aaaaaataaa cttccagtca gttttggctg gaagtttttt atttggtgca gatcttttca
     1081 gctgttattc atacaagttg atccattttt ctattattaa gtacatacca ttgttactct
     1141 ttgaatgtgg gggagaaaaa aataaaagga gctggtattt tgttagttat gagtaaagct
     1201 ttgtttttac ttccctgcac gttcatgttt attgttttat gttatttcaa aagaagaaaa
     1261 aaatggtgct ggatcgatgt tctactattc cttccctttt tcccgcttat ttattttctt
     1321 gaagaaatat gggatgatat ggcatgtgat tctgcttttc tttttagtca
//
LOCUS       DACDKL010000312         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDKL010000312 DACDKL010000000
VERSION     DACDKL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.319"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IV329_002802"
     rRNA            <1..>1355
                     /locus_tag="IV329_002802"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDKL010000313         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.322, whole genome shotgun sequence.
ACCESSION   DACDKL010000313 DACDKL010000000
VERSION     DACDKL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.322"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..1241
                     /locus_tag="IV329_002803"
     CDS             72..1241
                     /locus_tag="IV329_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8941901.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          485 a          208 c          218 g          439 t
ORIGIN      
        1 gtagaaaaaa agaaagtcca ttctgtaaaa ttaaagttac cacaaccgaa attttatagg
       61 aggactttct catgcccaat tttactacag aaattatgga aacactaatc aataaaggtg
      121 atttagatga attatttcga tgtcatttag aattagctgt taattcatta ttgcaggctg
      181 aattaacagc ttttcttgat tatgaaaaat atgatcgtgc tgggttcaat tcaggtaatt
      241 cccgtaatgg gaattattca cgttcattca gaacagagta tggagaatta aatttggtaa
      301 ttcctagaga tagaaatggt aagttttctc aacaaacttt acctgcttat aaaagaacca
      361 atgactcttt agaaaccact attattcagc tatttcaaaa aggaattact atgtctgaaa
      421 tctctgaatt gattgaaaaa atgtacgggc atcactatac gccacaaacc atttcaaata
      481 tgactaaaat agtttctgaa gatattattg cctttaaaga aaggtcctta gaatccagat
      541 actctgtcat ttttatggat gctactcaca ttcctttaaa aagacaaact gtttcaaaag
      601 aagcagttta tattgtaatt ggcattcgat tagatggaac caaagaggtt cttgggttta
      661 gtcttgctcc aactgaatct gcttatgttt ggaaagagat tcttcaagac ctaaaagacc
      721 gtggtttaaa agaggtttta ttagtcgtaa ctgatggttt aagtggcatt aacgatagta
      781 ttcatagtgt ttatccaaat gctcaatttc aacaatgttg tgtccatata tctagaaata
      841 tcgctcataa ggttcgtgtt agtgatcgac aagaaatctg tagcgatttc aaattagttt
      901 atcaagcaag ttcaaaagaa gaagcaaata atcaaataag gtttatgata gataaatgga
      961 aaaagcagta tccacgagtg gttaaattgc tcatgaatcc tgctatatta accttttata
     1021 atttccctcc gtcaatcaga agaactattt attcaactaa tttgattgaa ggttttaaca
     1081 aacaattaaa gaaatatacc aaaagaaaag agcaatttcc taatgaagaa tctctggaga
     1141 gatttctagt atctcaattc aacaactata atcaaaagtt tctatgccga gttcataaag
     1201 gttttaaaga aatacatgat acattagaat caatgtttta acttgatcaa cagaatgggc
     1261 ttttccattt acacataatt attgacacaa cctattctgg aaacttacaa ataaaagctt
     1321 attcttcagc caccttcggg tggttatttt
//
LOCUS       DACDKL010000314         1343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.323, whole genome shotgun sequence.
ACCESSION   DACDKL010000314 DACDKL010000000
VERSION     DACDKL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.323"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..486
                     /locus_tag="IV329_002804"
     CDS             <1..486
                     /locus_tag="IV329_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8941902.1"
                     /translation="LFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLITSFD
                     PLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKT
                     MIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERS
                     L"
     gene            483..>1343
                     /locus_tag="IV329_002805"
     CDS             483..>1343
                     /locus_tag="IV329_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8941903.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTD"
BASE COUNT          495 a          172 c          247 g          429 t
ORIGIN      
        1 ttatttcata ttttatttgg aaatatttta gctgtacaag atattgataa gtggattaca
       61 attggcgttt cggttttagt gttggttata gtagttctat tttttaaaga attattaatc
      121 acttcttttg atccattaat ggcaaaagca atagggatga atgtcagttt ttatcattat
      181 ctattgatgg ttctattaac tttagtagca gtaacagcta tgcaaagtgt gggaactata
      241 ttaattgttg caatgttaat tacaccagca gctacagcat atttatatgc aaatagcttg
      301 aaaactatga tatttatttc tgcaacagtc ggagcaagtt catcattgct tggattgttt
      361 attggttata gttttaacat agcagcagga tcaagtattg ttttaacagc ggcattctta
      421 tttgtaatta gttttctaat ctcaccaaaa caaaaatttt taagaaagaa ggagagatcg
      481 ttatgatgag aaaatggaaa gcagtactgg gaagtctggg gattttgatt gctcttttta
      541 tattcggggc atgttcaata aatagtaaag acaaagataa agtggcttcg aacgaaaaat
      601 taaaagtagt agttactaat tcgattttag cagatattac cgaaaatata gcaaaagata
      661 aaattgattt acacagtatc gtacctattg ggaaagatcc ccacgaatat gaacctttgc
      721 ctgaagatgt tcaaaaaact tcaaaagcag atttgatttt ttataacggt gttaacttgg
      781 aaactggagg aaatgcttgg tttacaaaat tagttaaaaa tgcgaacaaa gaggaaaaca
      841 aagactattt tgcagcaagt gatggcatag atgttattta cttagagggt cagagtgaga
      901 aagggaagga agatcctcat gcttggttaa atttagaaaa cggtattatt tacgctaaaa
      961 atattgaaaa acagttagcg gaaaaagatc ctgataataa aaaattctat aaagaaaatc
     1021 tagataagta tattgaaaaa ttggattctc tagacaaaga agctaaatct aaatttgctt
     1081 caattccgaa tgataaaaaa atgattgtta caagtgaagg atgctttaaa tatttctcga
     1141 aagcgtataa tgtgccttct gcttacattt gggaaatcaa cactgaagaa gaaggaacac
     1201 cagatcaaat aaaacactta gttgaaaaat tacgcacaac aaaagttccc tccttattcg
     1261 tagaaagcag tgtggatgat agaccaatga aaacagtatc aaaagatacc aatattccta
     1321 tctattcaac gatttttact gat
//
LOCUS       DACDKL010000315         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.324, whole genome shotgun sequence.
ACCESSION   DACDKL010000315 DACDKL010000000
VERSION     DACDKL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.324"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..820
                     /locus_tag="IV329_002806"
     CDS             278..820
                     /locus_tag="IV329_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941904.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            910..>1324
                     /locus_tag="IV329_002807"
     CDS             910..>1324
                     /locus_tag="IV329_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8941905.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DACDKL010000316         1323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.325, whole genome shotgun sequence.
ACCESSION   DACDKL010000316 DACDKL010000000
VERSION     DACDKL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.325"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IV329_002808"
     CDS             <1..197
                     /locus_tag="IV329_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941906.1"
                     /translation="YQSVRLVDFASDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQT
                     GEEPINAIQLFNRYEQENND"
     gene            212..643
                     /locus_tag="IV329_002809"
     CDS             212..643
                     /locus_tag="IV329_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941907.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            825..1130
                     /locus_tag="IV329_002810"
     CDS             825..1130
                     /locus_tag="IV329_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941908.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT          511 a          179 c          269 g          364 t
ORIGIN      
        1 cttatcaaag cgttcgcttg gtagattttg caagtgatga attggtatta acaacgatgg
       61 gcaacttttt agattatgtc ggagacaaaa aatggttgga aaaaattttg tctaaattga
      121 ttgcgaaaca aacaggagaa gaacccataa atgcgattca attgttcaat cgatatgaac
      181 aagaaaacaa tgattaaaga ggagagaaaa aatgaaaaga ttaaaacgaa gtatttttat
      241 tgtgctagca ttgattgatt ttgttgggac aggttgtgaa gcaattgacg ttcaaaaagc
      301 tcccttatcg gaaataaaac aagcaataac aaaacaagat gaagaacaac cgctaaatac
      361 acaagtagaa atagtgaatc aaaataaacc gagtttttct gaagaagatt tttcgttaga
      421 aaatggaacc aggcaatttt tttcagattt ggatcgttta aatcgggtag gtgtagcaag
      481 tgcacttttg cataaaagca ggatgccaac agaagaacga gaccgattat acattaagcc
      541 aactggctgg aaacaaaaga aaatgaagaa tggcgattat ctttataatc gttgtcattt
      601 gattggctat caacgtgcga caagtttccc cataagtatg taatacgaaa cgggaggatt
      661 aatcttaaga ttaatcacaa ctaacctatt tgacagttgg aatgaaagct atcatgtctg
      721 ctgaatcaac tggtctgata ctcctacatg caataaagtc tactaggaag ttattgtatg
      781 aagctaggta aaggcggcag aacaatttct aagtgtggaa tcatatgaag ttggatatgc
      841 cgggacgcta taaattactc agggtgagac tgtttttaat taaactgacg aacttgcgaa
      901 tgtacgggtc tataaccgac ttatattcga aagatataag attgcgaatg caaagaacat
      961 tatcgaaaag taagaatgtt ggtcatgaaa ttcgagatgg aagactagcg gtgtatctgt
     1021 caagagttgg taaaacatgt ttgattgatt cagatgagag tgggaatgcg acaaaacgtt
     1081 ttacagaaga aatagagcaa ctagcagaac tgaatatacg atatttttaa atactttagc
     1141 ggagaataaa aaaatcctct acagaaaact acggagggca acatgtgcta ttgtttcaac
     1201 aatacatttt taatttttac cataacaaaa aagtacttga aaaaaaacag aaatatgtta
     1261 aattaaaaat gaaaaaatgg aagcggttac ttattctttt gaatctaaga taaaaattca
     1321 aaa
//
LOCUS       DACDKL010000317         1323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.326, whole genome shotgun sequence.
ACCESSION   DACDKL010000317 DACDKL010000000
VERSION     DACDKL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.326"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..284
                     /locus_tag="IV329_002811"
     CDS             <1..284
                     /locus_tag="IV329_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8941909.1"
                     /translation="ENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFD
                     GQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            289..1236
                     /locus_tag="IV329_002812"
     CDS             289..1236
                     /locus_tag="IV329_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8941910.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          440 a          189 c          272 g          422 t
ORIGIN      
        1 ttgaaaatga agttactata gttaataaat caagatacac atctaggcaa cagacacctg
       61 aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta
      121 attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata
      181 ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt
      241 cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat
      301 cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa
      361 catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg
      421 aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg
      481 aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca
      541 gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa
      601 ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt
      661 tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct
      721 tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat
      781 gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct
      841 ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa
      901 gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat
      961 gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc
     1021 aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt
     1081 ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct
     1141 ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt
     1201 ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg
     1261 gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata
     1321 gaa
//
LOCUS       DACDKL010000318         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.327, whole genome shotgun sequence.
ACCESSION   DACDKL010000318 DACDKL010000000
VERSION     DACDKL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.327"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IV329_002813"
     CDS             <1..848
                     /locus_tag="IV329_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8941911.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACDKL010000319         1310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.328, whole genome shotgun sequence.
ACCESSION   DACDKL010000319 DACDKL010000000
VERSION     DACDKL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.328"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..>1310)
                     /locus_tag="IV329_002814"
     CDS             complement(150..>1310)
                     /locus_tag="IV329_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8941912.1"
                     /translation="SEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQYIQANAYER
                     SEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKALIAAMIEMV
                     ISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGSEYPFFMCDA
                     LYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFG
                     VKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKA
                     SELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLE
                     RLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          380 a          290 c          232 g          408 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct
//
LOCUS       DACDKL010000320         1305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.329, whole genome shotgun sequence.
ACCESSION   DACDKL010000320 DACDKL010000000
VERSION     DACDKL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.329"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(190..1215)
                     /locus_tag="IV329_002815"
     CDS             complement(190..1215)
                     /locus_tag="IV329_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8941913.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          373 a          220 c          260 g          452 t
ORIGIN      
        1 tagttttttc ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct
       61 atctaaaaca gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg
      121 atgagtgatt gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat
      181 aattctgttc tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag
      241 aatgattctt ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc
      301 tttgattcct aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga
      361 ggacaataag ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga
      421 ttgttctgcc actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt
      481 tggtctttct cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa
      541 gccgccgtct ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg
      601 gtaaccattg agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat
      661 atgacggtgt ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc
      721 cgtgtccact gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg
      781 aatattatat tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat
      841 aattcctcgg atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg
      901 tgtttttgcc gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga
      961 ttgaataaca tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa
     1021 tgctagaatc gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata
     1081 attcaattct ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg
     1141 gttatttaat actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata
     1201 aatagtaggt ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat
     1261 agtacaatag acattctttt ttgtctatga ttttgaaaat ttagg
//
LOCUS       DACDKL010000321         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.330, whole genome shotgun sequence.
ACCESSION   DACDKL010000321 DACDKL010000000
VERSION     DACDKL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.330"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            52..>1302
                     /locus_tag="IV329_002816"
     CDS             52..>1302
                     /locus_tag="IV329_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8941914.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDKL010000322         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.331, whole genome shotgun sequence.
ACCESSION   DACDKL010000322 DACDKL010000000
VERSION     DACDKL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.331"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..1004
                     /gene="yidC"
                     /locus_tag="IV329_002817"
     CDS             51..1004
                     /gene="yidC"
                     /locus_tag="IV329_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8941915.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            1126..>1302
                     /locus_tag="IV329_002818"
     CDS             1126..>1302
                     /locus_tag="IV329_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP8941916.1"
                     /translation="MYSRMEEDKISLDALVPKNHLVRKIDKLLSFDFIYLIVESTYST
                     IGRPSVDPVVLVKLV"
BASE COUNT          434 a          256 c          266 g          346 t
ORIGIN      
        1 tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct atgaataaat
       61 ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta tctggctgtg
      121 tgcggatgga ttcaaacgga aaccctgaca cttcaggaat cgtttatcga gtactcgtcc
      181 atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct tacggctggg
      241 cagttattgc aatgactgtg atcgttcgta tcatcatctt gccactaggt atcagtcaat
      301 cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag
      361 cacaacaaaa gctaaaagct gcaacaacac gtgaagaaca aatggcagca caagcagaaa
      421 tgcagcaagt atatcgcgaa aatggattga gtatgaccgg cggaatcggc tgtctgcctc
      481 tattgatcca aatgccgatc ttctctgcct tgtattttac ggctcgctac acagaaggta
      541 tccgtgaatc atctttttac ggcatcgatc taggctcacc aagtatggtg cttgtggcca
      601 tcgcaggggg cgcttatctg cttcaaggat atatttcaac aatcggtatt cctgaagaac
      661 agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga
      721 tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc agctgtctgc
      781 aaacattcat cacaaacgtt ataatgaaac cgcgtttgaa agcacaagtg gcagaagagt
      841 tgaaacaaaa cccgccaaaa caagtcgtta ctccaagaaa agacgtgaca gaatcagcag
      901 caaagccagc tgacaacaaa caagcaacaa aacaaatcaa acaaccaaaa aacagtacaa
      961 acaacaaagg ccgcaacgca ggaaggcagc agaagcggaa ataaaaatat atgacaaaaa
     1021 actgtagaca aataaaaaaa tttctttgtt ctacagtttt tttgttacaa ttaaagaaaa
     1081 cgttcgcatt tcgaggagga atgtataatg atgtccaaat aatcaatgta tagtcgaatg
     1141 gaagaagaca aaatttcgct agatgcactt gttcctaaaa atcatttagt ccgtaaaatt
     1201 gacaaattat tgtcatttga ttttatttat ctcattgttg aatcaactta ctcgaccatt
     1261 ggtcgcccta gcgttgatcc tgtcgtctta gtaaaattag ta
//
LOCUS       DACDKL010000323         1300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.332, whole genome shotgun sequence.
ACCESSION   DACDKL010000323 DACDKL010000000
VERSION     DACDKL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.332"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..>1300
                     /locus_tag="IV329_002819"
     CDS             18..>1300
                     /locus_tag="IV329_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8941917.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          405 a          283 c          261 g          351 t
ORIGIN      
        1 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
       61 acctaaatct tacctttcat gaagatgcct ttcgcaaaga gcgcattaat gacgaaacat
      121 gttttgtttt tgatggcaca ttgacctatc aaccagagga atgcttccat tgccactatc
      181 aaaataagca aacgatcatt aaatggggct ggaaaaaagt atccatctta ttgaatgatg
      241 tcagtaacta caagaccatt gtgcggatta acaaacagcg cttcaaatgt aaacactgtg
      301 gaaagacttt tttagcagaa gattcagtta gcgatcgtcg ctgctccatt gcccgcagag
      361 tcaaacaagc cattcttgag ctattaagtg agcctatttc tatgtctttg attgcacgta
      421 tgaagcacat ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaaccg
      481 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
      541 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      601 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctagaaaag
      661 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      721 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      781 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      841 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaatg
      901 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagcg gagatagtcg
      961 atcggctatt atattatgat gactccttaa agcgaggcta cgaagtctac caggtttttc
     1021 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
     1081 aggagttgcc agagcactat caaccagtgt tcacgacctt caagaaatat cgaacagaaa
     1141 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
     1201 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac cgagaacgaa
     1261 tctttttata tcgaggaaaa tatttcaaga aaactaagaa
//
LOCUS       DACDKL010000324         1275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.333, whole genome shotgun sequence.
ACCESSION   DACDKL010000324 DACDKL010000000
VERSION     DACDKL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.333"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(120..608)
                     /locus_tag="IV329_002820"
     CDS             complement(120..608)
                     /locus_tag="IV329_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941918.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(630..1232)
                     /locus_tag="IV329_002821"
     CDS             complement(630..1232)
                     /locus_tag="IV329_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8941919.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          404 a          291 c          184 g          396 t
ORIGIN      
        1 ccggcaggtg ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc
       61 tgtaaaccag agtccttctc gacccgtcgc ataaatttac tttaacacag ttcctatgtt
      121 tatgcgagag cttttttcat tttataatcg accatttgaa atccttgacc gatatctgaa
      181 ataagttctt ctgtaatctc aaatccttca tgcttataaa cctcgattgc ttgatgattc
      241 ccctgattca ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag ttcttcatac
      301 cagcgaaaaa ctgaagaagt caatccttga ccacgtactt tatttagcaa atataattta
      361 cttaaataaa tttttccatt tatctcttca taagcagtgt aaccgacagt cttttcttcg
      421 ctttttaata aaaagtagga cacaccttct gtcagttctt tttgaatctg tttttttgac
      481 tgataagttt tcagcatgta atccacttgt ttttgaccaa tgatcggcac aaaaacttcc
      541 ggccatactt cttgtatgac tgccatcagc tcatccagtt cattttctgt tacttttatt
      601 ttttccatac acccacgtcc ttttttacat tataaatgac cttgcgcttg atggaataaa
      661 aagagcatga tcatgctata cacacccacc gaccacggtt tcaagaaaat cacaatctgc
      721 ataaattccc taaaagataa tttcgtcaaa ccagctacta gacagatcaa gtcgtctggc
      781 gcaaagggaa gcagcaaggt aacaactagt acttttttaa tatctttcgt ttttcttgtc
      841 agcaattcat caaatttctg atactttttt ggcgataaaa taagctggac aaaggctacg
      901 ccatagtaac ggacaagcaa aaatccgatt gcttccccaa tcactaggcc aatacaacta
      961 tataaaagcc caaatccatt gccaaacatc agcatgcccg ctactgagga gatccctccc
     1021 ggcaaaatag gaatgatcac ttggatcact tgcaacagaa caaatatcat cactgcatac
     1081 ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat cccatgatga
     1141 taagcataaa gaatcaacac aaagaaaaaa aacagaccaa taatgggcat gatctgaaga
     1201 catttttgta aaactgatcc ttttttagac atctatattc cccacatctt ttttcattta
     1261 ttaaaactgc ctcct
//
LOCUS       DACDKL010000325         1263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.334, whole genome shotgun sequence.
ACCESSION   DACDKL010000325 DACDKL010000000
VERSION     DACDKL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.334"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..1097
                     /locus_tag="IV329_002822"
     CDS             18..1097
                     /locus_tag="IV329_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8941920.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          375 a          301 c          310 g          277 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaccc atgataacac agtttcacgc aagggaaaac
       61 atctttccta ctcagaacgt tgccaaatcg ctatcctgaa aaaggaaggc tattccaacc
      121 gtcaggttgc caaagtgctg ggacgtgttc cgcaaaccat aaacaatgag attgatcgcg
      181 gaaccatcac ccagttaaaa cgccaaacgc aaaatggaaa agtgtatgac acctactatt
      241 ctgtttatga cgctgatgca ggacaggcct tctatgaaaa gcaacgactg aactgtggca
      301 gacgccccaa atgggcgaac acaaatgcct tcattgattg ggcagatgac aaaatgttaa
      361 atgaaaaatg gtcgccggac gtcgtggtcg gatttgccag aaaacacgag ctgttcgatt
      421 gtgccatcat tcctggcaca accgctcttt atcaatggat tgaccgcgga atcatgcgaa
      481 caaagaacat ggacttgctt gaaaaactgt cccgtaaaat caaagcggtc cgcccaggac
      541 accgtcccaa caagcgtgtt ctcgggacgt caattgaaga acgccctaaa gaagttgaaa
      601 cacgggaaac gttcggtcat tgggaaatcg acacggtagt gggaaacaag accaagtcgg
      661 atgccgtgct gctgacgctg gctgaacggc agacacgctt tgaagtcatc ctaaagttga
      721 acgggaagga caccgaatcg gtggaccaag cggttcaaca gctaagagaa cgggctggcg
      781 ctaacttcga tcaattattt aagaccgtta catccgacaa tggctcggag ttttctgggc
      841 tacacgatgc tctgcaagat gtagctgacg tctatttcag ccacccctat gcttcttggg
      901 agcgtgggac gagcgaaaac cagcacaaat taattcgccg tttcgtcccc aagggaagcc
      961 cgattagtga ggtgagcgag aaccagattt tgcggatcca gcgttggatg aacgattacc
     1021 cgcggaaaat tttgggttat gcgacaccac atgatacatt cgtccaggca ttcaagcaag
     1081 aacggttggt ggcttgaccc attattcctt gataacgatc ctcactcggg acatggcttc
     1141 atgtctggaa agctggcctg gggccagaat cgtctgaaga ctatcatacc tcagttcatt
     1201 atcaaggcct cactgattta gaataacgag gcagttacgg tatcaaacaa atgagttacc
     1261 tgt
//
LOCUS       DACDKL010000326         1259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.335, whole genome shotgun sequence.
ACCESSION   DACDKL010000326 DACDKL010000000
VERSION     DACDKL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.335"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..1097
                     /locus_tag="IV329_002823"
     CDS             18..1097
                     /locus_tag="IV329_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8941921.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          332 a          342 c          356 g          229 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaacc atgataacac agtttcacgc aagggaaaac
       61 acctaaccta ctcagaacgt tgccaaatcg ccatcctgaa aaaggaaaac tattccaacc
      121 gtcaggttgc ggatgtcctg ggctgcgtgc cgcagaccat aaacaacgaa atcaaacgcg
      181 ggaccgttac tcagcttaac cgccagaatc agaacgggaa ggtctacgac tattatcatg
      241 acgtctacga cgccgatgcc gggcaggcgg cctacgagaa gaaccgcttg aactgcggca
      301 gacggccctt atgggccgac acggacgcct tcattgaatg ggcggacgac atgatgctgt
      361 tggaaaaatg gtccccggac gtggtggtgg gctttgccag aaaacacgaa ttgttcgacc
      421 cggccattat ccctgggacc accaccctct atcagtggat cgaccgtggc atcatgaaaa
      481 cgaagaatat ggacctgcta gaaaaactgt cccgcaaacc gaaatcgtcc cgtcatggca
      541 accgcccgaa taaacgggtg ctagggaaat ccattgaaga gcgcccggag gcggttggta
      601 cccggaaaac cttcggccat tgggaagtcg acacggtcgt aggaaacaag accaagtcgg
      661 atgccgtgct gctgacgttg gtggaacggc agacacgctt cgaggtcatt ctgaagctgg
      721 ccgggaagga tgccgaatcg gtcaaccaag cggtccgaca gctgagagaa cgggctggtg
      781 cgtacttcgg ccagctgttc aagacggtta cctcggacaa cggctcggag ttcgcgggac
      841 tgcacgatgc cttgcaggat gtggcaaaca tctacttcag ccacccctat gcttcctggg
      901 agcgcgggac gagcgaaaac caacacaagc tgattcgccg tttcatcccc aagggcagcc
      961 caatcggcaa cgtgagcgaa aaccaaatct tgcggattca gcgttggatg aacgattacc
     1021 cgcgaaagat tttgggctat gcaacgccgc atgacacgtt cgtccaggca tttaggcagg
     1081 aacggttgac ggcttgacac gtgggccctt gacaacgagc ctccctcggc atggcttgtg
     1141 tctggaaagc tggccggggc cagccagtcg ggagactacc atgcctcagc tcattgtcaa
     1201 gggcccactc gattagaaaa attagacggt tggtggtaac gagcaaccgg gtcaccatg
//
LOCUS       DACDKL010000327         1258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.336, whole genome shotgun sequence.
ACCESSION   DACDKL010000327 DACDKL010000000
VERSION     DACDKL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.336"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(342..698)
                     /locus_tag="IV329_002824"
     CDS             complement(342..698)
                     /locus_tag="IV329_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8941922.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(712..1122)
                     /locus_tag="IV329_002825"
     CDS             complement(712..1122)
                     /locus_tag="IV329_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8941923.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          405 a          229 c          177 g          447 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
       61 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      121 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      181 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      241 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      301 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      361 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      421 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      481 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      541 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      601 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      661 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      721 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      781 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
      841 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
      901 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
      961 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1021 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1081 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1141 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1201 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatat
//
LOCUS       DACDKL010000328         1251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.337, whole genome shotgun sequence.
ACCESSION   DACDKL010000328 DACDKL010000000
VERSION     DACDKL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.337"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1251
                     /locus_tag="IV329_002826"
     CDS             <1..>1251
                     /locus_tag="IV329_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8941924.1"
                     /translation="KQLFDISTELDSHQFNEFRNSIRLSDYEFYYTSKSNLNFCINEN
                     YDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLREMKNNRNP
                     VKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSIIGSVYSP
                     NLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACRSRELL
                     NIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNVLKSFN
                     GKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEI
                     LIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPS
                     EHRLYKSTAQEKGQARKEKLSSLFN"
BASE COUNT          467 a          184 c          205 g          395 t
ORIGIN      
        1 aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga atttagaaat
       61 tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct aaatttctgt
      121 atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc tagtaatcct
      181 gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat tttcaagcat
      241 atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa aaacaatcga
      301 aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag
      361 tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc tgaaattagg
      421 aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt gtattctcct
      481 aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa atttctttca
      541 atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct aaatatgatt
      601 tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact acttaatatt
      661 atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac tctgttcgaa
      721 ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc tgtacattta
      781 gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg caaatctatc
      841 gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa aaagaacaat
      901 ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg attgcttttt
      961 tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc tcaaatagta
     1021 aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa tgttggtagt
     1081 acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag aaagtgtgct
     1141 attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta taaaagcact
     1201 gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa t
//
LOCUS       DACDKL010000329         1242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.338, whole genome shotgun sequence.
ACCESSION   DACDKL010000329 DACDKL010000000
VERSION     DACDKL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.338"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            10..483
                     /locus_tag="IV329_002827"
     CDS             10..483
                     /locus_tag="IV329_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8941925.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            492..719
                     /locus_tag="IV329_002828"
     CDS             492..719
                     /locus_tag="IV329_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941926.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          390 a          196 c          208 g          448 t
ORIGIN      
        1 gggataaaaa tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc
       61 gtatttattt tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc
      121 aattttgatt ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg
      181 atcgttattg cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt
      241 tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc
      301 gccttttttg gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt
      361 ttgatgttgg taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc
      421 gtgctggaaa aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg
      481 tgaggtgcga catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga
      541 gtgtgactga attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa
      601 atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt
      661 gtcaaccagg tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa
      721 ataaagtaga acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta
      781 gaagttattt acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata
      841 aataagatat gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt
      901 tcctgataca actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt
      961 attcattcca ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg
     1021 ccattctgtt tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact
     1081 cttactccat tcttttttag taagctccca tatatactgt aaagccttct atatattatg
     1141 tcatgttgct ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat
     1201 ggacaattct tttttatttt taacattatt ttgataataa tg
//
LOCUS       DACDKL010000330         1239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.339, whole genome shotgun sequence.
ACCESSION   DACDKL010000330 DACDKL010000000
VERSION     DACDKL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.339"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..833)
                     /locus_tag="IV329_002829"
     CDS             complement(<1..833)
                     /locus_tag="IV329_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8941927.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     S"
BASE COUNT          365 a          253 c          194 g          427 t
ORIGIN      
        1 tcactgtaaa cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg
       61 actaactcat aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt
      121 aaagtttggg gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca
      181 agcatttcta aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc
      241 aaactgccct tttcttccaa gcgggctcca tcaaatccat aagaaacgtt gattacttga
      301 tttggatata aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc
      361 gctaactttt gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca
      421 cctgccacat aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg
      481 acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct gctgatttct
      541 gaaggcagat atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat
      601 ccatatctaa acgccgtttc tgtcactctt gatccatcta ggagatcgcg attagccaaa
      661 gccaatctac ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg
      721 cgttttaatt gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct
      781 tctgtcaaat gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc
      841 ttcctccttg cttgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag
      901 gaaccaattt agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc
      961 gtttcgtaac ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa
     1021 ttttggggaa gacacattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt
     1081 taatccgctt actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc
     1141 tgcttggatt tttctttgcg atacatcata cctcttttat caattggaac aattatttat
     1201 ttctaacaaa atcacttcat ttttgtcaga taaataaaa
//
LOCUS       DACDKL010000331         1221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.340, whole genome shotgun sequence.
ACCESSION   DACDKL010000331 DACDKL010000000
VERSION     DACDKL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.340"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..392)
                     /locus_tag="IV329_002830"
     CDS             complement(<1..392)
                     /locus_tag="IV329_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8941928.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEV"
     gene            complement(551..817)
                     /locus_tag="IV329_002831"
     CDS             complement(551..817)
                     /locus_tag="IV329_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941929.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(807..1163)
                     /locus_tag="IV329_002832"
     CDS             complement(807..1163)
                     /locus_tag="IV329_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8941930.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          335 a          242 c          152 g          492 t
ORIGIN      
        1 acttctatat actcttcagc caaaacttgt ggaactggtt catctaagaa aaaaacccct
       61 acctcgcctt tgctattttt ataaaagtaa tcaatttctg ctatagttcc cgatctataa
      121 ttatcaactg gagaacccaa cttatggcca aaaatcgcta ataatatatc actattttct
      181 acaatttgtt tattaataac actttgccca gagctattat ttatataagt aggaatcgca
      241 ttcgtttccc atcgaattgg catcaaaatt acttttgtat tatctgtatt ttctatattc
      301 catctatata tggtttcttc tacttcattc ctctttgctg ctatatcacc tggtgatgct
      361 ataaatactt ttattagatt tatggttttc atttattgta cctttctctt tatgtctctt
      421 taaagtaatt tttgtataaa atttattgac ttttacacta taggacaact tttatctatt
      481 tagtacaaaa aggcccacta aactaattga ttagttagga gcctattaca ggtatatttt
      541 caactcgctt ttaagcaaat tctactacca gctttttgcc taatgcccta gcgatatcat
      601 caagtttatc gaaagatacg ttatcccctc tctcaattcg agcaatagta gattgagttg
      661 tatgtgcttt ttgtgcaagc tccgcttgag ttaggcctgc ttcctcccgt gcatgataaa
      721 gagcaaccgc agtttcgagt tttcttcctt cacggtcata tgcttctgca aaatctttgt
      781 ctttttgctt ttcttcaatc aattttttaa tcatcatttg tgaaccactc ctctctaagt
      841 tcttttgcat gtttaagttc acttattggt gttttctccg tttttttagt aaatccatgg
      901 gtaatgacat accttccatt gaccacatgg aaatagatag ctctttggat gtttgatcct
      961 accttacttc ttaattcgta caaattagtg tctaattttt tcacccattt caaccgttga
     1021 gctactagca tcccattctt ttctgtttct tctattgtac gtaaaagctt cgcactatct
     1081 ttttttggta atgtttccaa ccattctaaa aattcatcat gaccattagg tcttttaaat
     1141 gtttcaaaag atattttttc catacccaat tatatcatat attgatttta tattatagta
     1201 tataacataa aaattcgaat t
//
LOCUS       DACDKL010000332         1217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.341, whole genome shotgun sequence.
ACCESSION   DACDKL010000332 DACDKL010000000
VERSION     DACDKL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.341"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IV329_002833"
     CDS             complement(26..466)
                     /locus_tag="IV329_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8941931.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IV329_002834"
     CDS             626..1132
                     /locus_tag="IV329_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8941932.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
BASE COUNT          429 a          179 c          238 g          371 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttc
//
LOCUS       DACDKL010000333         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.343, whole genome shotgun sequence.
ACCESSION   DACDKL010000333 DACDKL010000000
VERSION     DACDKL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.343"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV329_002835"
     CDS             <1..1076
                     /locus_tag="IV329_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8941933.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDKL010000334         1190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.344, whole genome shotgun sequence.
ACCESSION   DACDKL010000334 DACDKL010000000
VERSION     DACDKL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.344"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(172..786)
                     /locus_tag="IV329_002836"
     CDS             complement(172..786)
                     /locus_tag="IV329_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8941934.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          380 a          238 c          210 g          362 t
ORIGIN      
        1 acaaacttta aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa taactaaaac
       61 aaaaaaagta gtcgttgtta atcctcatta ataatcgttt tctttttttc aaacgctcca
      121 tttttatgtg gaacattatt tttttaagga actttgtcaa catgtggata actattttcc
      181 acttgtgtcc tggactagct gttttgctgt aaaatacaaa acttgctgca gcaaaaagat
      241 cgttgcaaga gaggctgttc cgcagatcgc tggatctaga tttggcaaag tcaagactgc
      301 atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca aattcaaata atcgcaaacc
      361 actgctatgt tgcgatttga tacttcgtga caaatcaaat ccagtaacaa tgattaatgg
      421 atgaccattt tctttgatcc actgagctag ttcgatgatt gccgggtttc ttccttcatt
      481 cgaaagaaga agaaaaatgt cttctgattt gacagaaagc tgatcaataa ttgcctgtcc
      541 tgcaccttct agcgtttcat agacacctcc ggctggattt tcaatcaata tacttggtac
      601 aagatccggg gtttgatccc aaaattcaaa tgctacacca cttagtgtgc ggctagcata
      661 tatctgcaat ctaccacctt gattaatttg tttcgcgatc caagtgccag cttgaagcat
      721 cagcggctct tctatcctta atttctcttc caattggtga taatagtcaa aataacataa
      781 actcatttat ctctcccgcc tagctatact tgacatgaat agaaacagct gcctaatcta
      841 aggaagtaca taaaaaaaca gtagttagac agtaacacat gtggtctttg ctgtttcaaa
      901 cacaaaaaag gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg
      961 cagaccatgg aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa
     1021 ttaggtcgat gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac
     1081 tggtcattcc ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg
     1141 tttctatctg aatttattgt aagtaatctc ttatccaagt gcaagcaaga
//
LOCUS       DACDKL010000335         1185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.345, whole genome shotgun sequence.
ACCESSION   DACDKL010000335 DACDKL010000000
VERSION     DACDKL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.345"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IV329_002837"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IV329_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IV329_002838"
     CDS             complement(545..739)
                     /locus_tag="IV329_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941935.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          419 a          197 c          164 g          405 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaac
//
LOCUS       DACDKL010000336         1172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.346, whole genome shotgun sequence.
ACCESSION   DACDKL010000336 DACDKL010000000
VERSION     DACDKL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.346"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..>1172
                     /locus_tag="IV329_002839"
     CDS             62..>1172
                     /locus_tag="IV329_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8941936.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREK"
BASE COUNT          385 a          229 c          230 g          328 t
ORIGIN      
        1 tatcgttctc tagtatgatt aaatcaccac aaaataaaca actgagagga cgatacgcta
       61 tatgaatgat tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga
      121 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      181 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      241 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      301 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      361 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      421 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      481 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      541 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      601 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      661 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      721 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      781 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      841 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      901 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      961 caaaacatgg aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat
     1021 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
     1081 agttgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     1141 cgaaacgtta gatgacagct ttcgggaaaa gc
//
LOCUS       DACDKL010000337         1168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.347, whole genome shotgun sequence.
ACCESSION   DACDKL010000337 DACDKL010000000
VERSION     DACDKL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.347"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..441)
                     /locus_tag="IV329_002840"
     CDS             complement(<1..441)
                     /locus_tag="IV329_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8941937.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADSVDCSL"
     gene            792..1091
                     /locus_tag="IV329_002841"
     CDS             792..1091
                     /locus_tag="IV329_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8941938.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          424 a          167 c          197 g          380 t
ORIGIN      
        1 gagggaacag tctacagaat ctgcgcttga taagacagag tgctcaaagg tactttcttt
       61 tatgtttgaa ccagataaat ttacatagtc cattttacaa ttctcgaata acgtgttccc
      121 aatataactt tccatcaatt tagtacctgt taatttacaa ttgacaaatt tcacgcgatg
      181 caacatgctt tttgtcaata tacagcctga aaaatcacag ttttcgaata tacagtcggt
      241 aatctccatt ctttgaaaat cattggaagc aaaaaatgca tacttgaaaa tagatttgta
      301 aataattgga tggcagatta tttggttttc gatgacctct ttttgtgaaa ggatattata
      361 cagttcaaaa tcttcgtttt catagatatg aaaaaaggat cctttattca agcttgcttc
      421 attaacttta ggaaaaatca tatttaatta cctccatttt cataaagtca tattaacaaa
      481 agctaaacca acttacatta gcagaaaatg agataggttt tcgccttttt cgataacgat
      541 agtgtcgcta tctcttgaaa cgttcttaga attgccctga gagacacaaa ttaggcgtaa
      601 ttctaataaa aatcttagaa taaaaatttt tactgattta atttagaatg tttttaaaat
      661 aaaaatcgtt gccataacag tgtattgaaa ggttttaaca atccagtaat gatttaaaaa
      721 aatctagatg tagcgtatac tttgtttata gaatgtagtg ggaagaaata tatttttggg
      781 aggaaatcgt gatgtctaaa agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc
      841 tcgttttaaa tccagagatc aaagaaaagg aacgtatact tttgatagac gcaaagacag
      901 gcttagaaaa aggtcagtat tatcctaaag tcataaataa tttggaacgt agtttacgtc
      961 cgttagcaat caggggagaa ttgtctaaac cagtatcacc gttttatatg gagatatcaa
     1021 caataggaaa atttgaaaaa gaattaggtc gaggaatggc ttcggcaccg attacttttg
     1081 ggcatttata aaaacgagtt attcatctca atgaattaaa aagtatagtc aatctcataa
     1141 tgcaggctag gttcatttat ttgaacag
//
LOCUS       DACDKL010000338         1167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.348, whole genome shotgun sequence.
ACCESSION   DACDKL010000338 DACDKL010000000
VERSION     DACDKL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.348"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1167
                     /locus_tag="IV329_002842"
     CDS             <1..>1167
                     /locus_tag="IV329_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8941939.1"
                     /translation="KKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETIVR
                     FEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKVSL
                     SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFICAD
                     GETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVID
                     RFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWK
                     SFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPET
                     LDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFEN"
BASE COUNT          385 a          230 c          227 g          325 t
ORIGIN      
        1 agaaaaagac gttgatcgtc gatgctgttt tctcacctgc tccccatacc tgtagaaatt
       61 gtggttctac tgtggtagat ggaaacggga aagtaattgt agtgaaaaat gggaagaagg
      121 aaacgattgt ccgctttgaa caatacaatc acatgccttt ggttatgcgc ttaaaaaagc
      181 agcgctatac ctgtaaaaac tgtcgaaccc attggacggc tcaaagttat tttgttcaac
      241 ccagacattc aatcgcgaat catgtcagat ataaaattgc ttctttactg actgaaaaag
      301 tatctttatc ttttattgcg aaaaattgtc aggtatcttt gaccaccgtt attcgtacgt
      361 tgaaagagtt taaaagctac ttaccaaagc aatctaagag gattctcccc agagtattga
      421 tggttgatga atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag
      481 atggcgaaac aggacaatta attgatgtct tgcctacgcg taaattgcct cgattaacaa
      541 gctatttctt aggctgcgct aatcctgaag aagtaaaatt tttagtcaca gacatgaacg
      601 ccgcctactt ccagcttacc aaacgtattc tgccaaatgc gaaagtagtg attgatcggt
      661 ttcatattat caaacacatg aatcaagcct ttaatgagtt gcgtatccgt gaaatgaatg
      721 aacttcgtaa agtaggacag aaaagtcagg cagaaaaact gaaaaagaac tggcgctttt
      781 tgctaaaaaa tcgtgcaaac atcaaccatt atgaatacaa aacatggaaa agttttcgag
      841 caccaaaata cccatttctt acggaagcga tgatgattga tcgattgctt ggcttttcta
      901 cgtccctgaa ggaggcgtat ccctattttc atgaattagt tgaagccttt cgagacaaag
      961 accctgactt atttttctcc ttattggcag aacttcccga aacgttagat gacggctttc
     1021 gggaaaagct ccaaaacctt ctgacctatg aagaaggcat caccaacgcg atgatctatc
     1081 cttacaccaa tggaaaaata gaagcgaaga atacccatat aaagacaatg aaacgagtat
     1141 cctacggatt taaatcattt gagaaca
//
LOCUS       DACDKL010000339         1160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.349, whole genome shotgun sequence.
ACCESSION   DACDKL010000339 DACDKL010000000
VERSION     DACDKL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.349"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..838)
                     /locus_tag="IV329_002843"
     CDS             complement(59..838)
                     /locus_tag="IV329_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941940.1"
                     /translation="MRLAINVLDKSEKSIAQIQNGFIENTPDVIPPNWSETMVEKPVN
                     LEIFDKSVSVAENDHYFTFYADGLKEYERIENQLIITLFSTTGELGKPNLAWRPGRAS
                     GDTTNEGHVMMETPLAQEIGEYKVTFGFNVEEGRFNEFKVAKQAEKRLEQSISYQKQK
                     LNVFIHRLDNKIWPLQNELDVPRKFSLIELPEDLIVSSIYPSYFHEQGFVLRLANPTE
                     QEKIVPEAILSLGTVVNALENKQELTTIPPYDYLSILINER"
BASE COUNT          355 a          206 c          177 g          422 t
ORIGIN      
        1 agagggtaaa aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt
       61 atctctcatt tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt
      121 tattctccag tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt
      181 cttgttctgt tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat
      241 aaatcgatga tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat
      301 caagttcatt ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt
      361 tctgcttttg ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga
      421 actcattaaa tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct
      481 cttgagctag cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc
      541 gcccaggtcg ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta
      601 tgattaattg attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat
      661 aatgatcatt ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct
      721 ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt
      781 tttgaatttg tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac
      841 gatgcgagta aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca
      901 gactgaacga tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt
      961 ttaaatcttt aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt
     1021 tatatacttt agctgtttct aattttagag atagttctgt atctccttct aatggtgaat
     1081 aatcatacgt atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc
     1141 tatctccaac tatcaaattc
//
LOCUS       DACDKL010000340         1160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.350, whole genome shotgun sequence.
ACCESSION   DACDKL010000340 DACDKL010000000
VERSION     DACDKL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.350"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..833)
                     /locus_tag="IV329_002844"
     CDS             complement(<1..833)
                     /locus_tag="IV329_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8941941.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RS"
     gene            complement(852..>1160)
                     /locus_tag="IV329_002845"
     CDS             complement(852..>1160)
                     /locus_tag="IV329_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8941942.1"
                     /translation="IFYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEES
                     KEDLIATIVDILGYAISPEYLFNVLADQAKQATFQLNDLNKAFLASHIYGKILSAKFE
                     "
BASE COUNT          347 a          237 c          169 g          407 t
ORIGIN      
        1 gaacgactgt ctaatgttgg aagtgttagc tttacaggta ttcttctcaa caaagttgat
       61 aatagtttat cctcaggtgg ttctgatgtt gcaaatataa tccagcaact tgatgtgtac
      121 cagttttcat tatcacccat ccgatgaaat ttcctcgtat ccataaactg aaaaagtttc
      181 tcctgacatt cattgctcaa tgcatgaatt tcatctaaaa ataagactcc attattagct
      241 aattctgcta aaccctttct tccatcttcg gctcctgtat atgcaccttt tacatgccca
      301 aaaaaattgg acaagaaaag ttctggatta ttggcatatt cactacaatt aactgaaatg
      361 aaagatgcct tctcttctat ataattattg gccaacaaat ccttatacac ataatttgct
      421 aaatagcttt ttccagttcc agtctcgcca tgaagtaaaa tgggtaagcc atttggcgga
      481 tagtagattg ctgctttaat ttgagcaatt acatttcgca gacttccttg acttccgata
      541 accctttcta ttccaaccct aaaagagcta tcataccatt tttccgcttc ctctgaagag
      601 ctaaaaattt gttgatttgg aactttctcc ggtaaagaaa agaagattac tggacgagta
      661 ttgatttttc caaattctcc atcttttacg aattcattca aatattgaga gacaatatta
      721 cgactaacat ttaatttttc agatattttc tgtgctgtat tgttccccca tttattttta
      781 atctcagagt tctctatgac ataattgaca atgcgttctt ttaattcttc catcacagca
      841 cctcctcgac actattcgaa ttttgccgac agtatttttc cataaatatg acttgctaaa
      901 aaggctttgt ttagatcatt caattggaac gttgcttgtt ttgcttgatc cgctaagacg
      961 ttgaaaagat actctggcga aatcgcatac cccaaaatat caacgattgt tgcaatcaga
     1021 tcttcttttg attcttcatc tgaaagtaat tcacgataca acatcgtttg tttgcttact
     1081 gtgtcatatt cctctaagga ttcatctgct aaaagaacca cttgttccaa caaacggtcg
     1141 gataaatatt tatagaaaat
//
LOCUS       DACDKL010000341         1147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.351, whole genome shotgun sequence.
ACCESSION   DACDKL010000341 DACDKL010000000
VERSION     DACDKL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.351"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(33..479)
                     /locus_tag="IV329_002846"
     CDS             complement(33..479)
                     /locus_tag="IV329_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8941943.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(572..817)
                     /locus_tag="IV329_002847"
     CDS             complement(572..817)
                     /locus_tag="IV329_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941944.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(933..>1147)
                     /locus_tag="IV329_002848"
     CDS             complement(933..>1147)
                     /locus_tag="IV329_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAP8941945.1"
                     /translation="AIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDMAECLAM
                     DHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT          339 a          241 c          169 g          398 t
ORIGIN      
        1 attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg
       61 tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac
      121 tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt
      181 ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg
      241 tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg
      301 taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc tccatcaatg
      361 tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg
      421 acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact
      481 aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac
      541 gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct
      601 caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt
      661 caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt
      721 ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt
      781 ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact
      841 ataccacata tttttaaaat tttcaagttt accataaaaa aaaaaagaaa tgcaataaaa
      901 ataaataaga agctcattaa gatttttcta aattattcct cattataaaa tttcaagtgt
      961 ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg atccattgct
     1021 aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa aattttagct
     1081 tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa aagatgctga
     1141 attgcta
//
LOCUS       DACDKL010000342         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.352, whole genome shotgun sequence.
ACCESSION   DACDKL010000342 DACDKL010000000
VERSION     DACDKL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.352"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV329_002849"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV329_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8941946.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDKL010000343         1140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.353, whole genome shotgun sequence.
ACCESSION   DACDKL010000343 DACDKL010000000
VERSION     DACDKL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.353"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..651
                     /locus_tag="IV329_002850"
     CDS             1..651
                     /locus_tag="IV329_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941947.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            838..>1140
                     /locus_tag="IV329_002851"
     CDS             838..>1140
                     /locus_tag="IV329_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8941948.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVG"
BASE COUNT          373 a          145 c          230 g          392 t
ORIGIN      
        1 atgtttataa agggagggca aaaaatgtgc ttttttacaa cttttactat cattatgatt
       61 gcagtacttt ttatttttct agatatagca aagcgaaata cagcattttt attatatcgg
      121 gttttacttc gagcaggtct cataacattt atatcgatcg ttggattttt tctattcaca
      181 gttattgtat ttatttggag aactacagca ccaccattgc cagagattac atatggagaa
      241 tttccgttta gtcttgaata tgaaatagat gaggaaagaa ttttgattga agatacggtg
      301 attgcagaat ttataggaag ttttagaggg aatgtgacaa ctcaacctag acgaagttgg
      361 agtataaaac ttttaaataa tagtggagag ttgaaagcat ttcattatga attcttaata
      421 atagaaagtg aagatgtaac aatcacttta agtagtggct ctgctggata ttttatgaat
      481 gatggtggaa gcaagccaca gctcacagtc cgtgatcgta ttgcaggttt tatgcatttt
      541 tctcactatg acttagaaga agctcgtaaa atacttgcag aatatgggat cacattaatc
      601 aattgggaat atgcgccacc gataagaaat tttttcaatg attattatta gtcttataaa
      661 aaacagaatg taaaatttat gtgcgcgagt atttagtttt ccattttttg tttgatagat
      721 atatagcatt gttgaagcat gggatagtgg tgccaatttt atcaatgagg ttggtcaggc
      781 agttaacata attggtgatg ctataaatcc atttaattgg aattggagga attaggtgtg
      841 gaaacagtga gattatggat attcatgaaa aattttcgtt cggctttgag atatgttatt
      901 ctggaatcaa aatcaggtta ttatctgatt gacacagatc actatacttt ttggggaggc
      961 tacctttttc cgatttttaa ttggataatc ccccataagg ttatgaaaat taacttgcct
     1021 gcagaagaaa taaatagatt aatactcaac aaagatttac aaaggaaaag agagaaagaa
     1081 cattctaatg ttgcttggat aggtccagca tttgcagtag tgacttcttc aattgtcggt
//
LOCUS       DACDKL010000344         1128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.354, whole genome shotgun sequence.
ACCESSION   DACDKL010000344 DACDKL010000000
VERSION     DACDKL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.354"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(425..784)
                     /locus_tag="IV329_002852"
                     /pseudo
     CDS             complement(425..784)
                     /locus_tag="IV329_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          363 a          196 c          208 g          361 t
ORIGIN      
        1 ggatgaaacc tttattcaat ccgttgcctc gaaaagaaac catattccaa gaaaatcatt
       61 aaattatcga acaccattgg aagtattttt gagctgcata gataaagaca tattgtctag
      121 cttaatttga caaatcagat gtctataaaa ttcgttagaa aatacaattt atagatatcc
      181 aattgatttg taaacaaaaa ggagttgtca actaagccac tcctttttgt tgtaattttt
      241 cccaataaga aagtctacta ctttaggaag ataatagtaa gatagaaatg ttgcccccaa
      301 aattattatc cagctggata taattaatca aataaaaagg atcaaaaatg tagtgacccc
      361 caaaagttag acttttttgg agtggagaaa tccactcttt ttttcatgct ctaaaatgat
      421 atttttacac aaccgtaacg gttgttttac gaaattggac gggactttgc cagtttaatt
      481 tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct gattttaatt
      541 cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag ccaaaaaaat
      601 tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga aagattttgt
      661 actccttcaa tttgtttcta taagctttca tttgatacga ccaaccttga tctgagtgaa
      721 acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa ccttccatta
      781 ccgcacgtgt ttctgaatgg tggtagcgac cgatttgaac ggctagggcg gaaatacggt
      841 gcaaatgcac ggtggtttca tgcaatgctt acagggcgta ttttgtaagg gtatgtaacg
      901 gagataatcc cgacggtaat ggatgtgaga ggccaagccg acgctgacgg tagggcaaac
      961 aaacatagtt gcactctagc gatcgttatt tatttttaac ggtcaaacta gggtacaact
     1021 atgcttaaaa cacttcgcct caacttctgg tagcaaaagt gattttgaaa gtcaaaatat
     1081 tttttatgag ttatttataa ttcgccgttt gtataattaa gcgagaca
//
LOCUS       DACDKL010000345         1124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.355, whole genome shotgun sequence.
ACCESSION   DACDKL010000345 DACDKL010000000
VERSION     DACDKL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.355"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..943
                     /locus_tag="IV329_002853"
     CDS             <1..943
                     /locus_tag="IV329_002853"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8941949.1"
                     /translation="KKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAEG
                     EALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIMR
                     QIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIAL
                     KLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKVR
                     NAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPSI
                     VDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          392 a          174 c          239 g          319 t
ORIGIN      
        1 gaaaaaaaca agtcgtaaag tagtaatcgt cggtacaggt tttgttggaa caagtatcgc
       61 ttatgcaatg atcaaccaag gaatctctaa tgaattagta ttgatcgatg tgaatcaaga
      121 aaaagcagaa ggggaagcat tggacctttt agatggtatg gcttggggcg atgaaaatgt
      181 ggctgtatgg tcaggtggct atgaagaatg taaagatgct gatatcgttg ttatcacagc
      241 tggtatcaac caaaaacctg gtcaatctcg tttagattta gttaaaacaa atgcgtcaat
      301 catgcgccaa atcgtaaaag aaatcatggg atcaggattc gacggtatta tcgttgttgc
      361 ttcaaaccca gtagatattt tgacatatat cgcttggaac gaatctggtc tgccaacatc
      421 acgtgttatc ggtactggaa caacattgga tacaactcgt ttccgtaaag aaatcgcatt
      481 gaaattaaaa gttgacccac gcagcgtcca cggctatatc ttaggtgaac atggtgattc
      541 agaagttgct gcatggtcac atacaactgt cggtggtaaa ccagtgtttg aaattgtaga
      601 aaaagaccat cgtattgccc aagatgaatt agatgtgatt gctgataaag tccgtaatgc
      661 tgcttatgag atcattgatc gtaaaaaagc aacttactat ggtattggta tgagtactgc
      721 acgtatcgta aaagctatct tgaataacga acaagctgta ttgcctgtat ctgcttattt
      781 aactggtgaa tacgacgaaa aagatatctt tacaggtgtg ccatcaatcg ttgacgaaaa
      841 tggtgttcgt gaagttgttg aattatcaat caacgaagaa gaaaaagcaa tgttcagcaa
      901 atcaacaagt gctttaagag aagtattgaa cactgtatta taagaaacaa tgaatatgaa
      961 ctaaaaatcc taaaactgct taacagctga aatagctgtt aagcagtttt ttattgatat
     1021 ctaataggta agcaaataaa cagaatggtt aaaattaatc ggtccgaaaa tgtatccgta
     1081 catatgaaca tatgaaattt aatctgttga ttacaataag cgaa
//
LOCUS       DACDKL010000346         1123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.356, whole genome shotgun sequence.
ACCESSION   DACDKL010000346 DACDKL010000000
VERSION     DACDKL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.356"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..482
                     /locus_tag="IV329_002854"
     CDS             <1..482
                     /locus_tag="IV329_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8941950.1"
                     /translation="SAIDRIHWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLAS
                     FQKKYSIVLYKYLAMYYKKYLIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTI
                     SNFDRRVLRESVEEINEKSMLNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            674..1072
                     /locus_tag="IV329_002855"
     CDS             674..1072
                     /locus_tag="IV329_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8941951.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          403 a          134 c          218 g          368 t
ORIGIN      
        1 tatctgctat tgatcggatt cattgggatt tagaagagca tagtgtatct ttcagttttt
       61 cagaagatgt aattccctta attgtctcaa tcaaaaagaa gtatccgttc tttgtttttg
      121 aagacttagc atcttttcaa aaaaaatata gcatcgtgtt atacaaatat ttggcgatgt
      181 attataaaaa atatcttatc gccaaaaata aaggagattt tgagaaagct gaaatatata
      241 agaatcccct gattgaaata gttaaaattc gtgagattac gaaaacagaa aatgagtata
      301 aaacaatatc gaattttgat agacgtgttt taagggaatc agttgaggaa attaatgaaa
      361 aatcaatgct gaatattgat tttgctaaag ttagaaaagg cagaaattac aattttgctg
      421 tccagtttaa tatttctagt gagtttgatg aaaaaactta tagagaaaaa aaattaggtt
      481 aatgcaaaat atatcttact cgtttgaaga tgcttcagac gtatgctaag atatatagtg
      541 ccttgggagc aaggtattaa tctcctttag aggttaatta gcttgtgatc gcctatcaat
      601 ttttttggta ggcgattctt ttttttgaaa caaaaaagca aaaattattt tttaattaga
      661 gaggtgcaat gcgatgaaat actttttttg tgcagtattc gaatggaaag gaaatgaata
      721 tgcagttaaa ttcccagatt ttccagactt atatacacat ggtgaaactt tagaagatgc
      781 ttttgagatg gcgcaagaag tattggaagg atacttggtg cttttagagt tgcaagaaaa
      841 agaaattcca tcgcctacgc ctgttcaaga gttaaatgca agaatcaagc gatctgagaa
      901 tgttaattta tttgtcgtaa aagctaatac gaaagttgct aaagctcgaa acaagcaaaa
      961 ttatgatcga aaatcagtga ctgtggaaca ctatttaaat gttttagcgg ctgaagagaa
     1021 tattaatctg tcgaaagctt tgaatgattt gttgagagaa aagttgattt gatgtctctc
     1081 agatgatcat attaagcaaa aacagcttgt tttaggacgt atg
//
LOCUS       DACDKL010000347         1122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.357, whole genome shotgun sequence.
ACCESSION   DACDKL010000347 DACDKL010000000
VERSION     DACDKL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.357"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..200
                     /locus_tag="IV329_002856"
     CDS             15..200
                     /locus_tag="IV329_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941952.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            212..460
                     /locus_tag="IV329_002857"
     CDS             212..460
                     /locus_tag="IV329_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941953.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            491..670
                     /locus_tag="IV329_002858"
     CDS             491..670
                     /locus_tag="IV329_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941954.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(713..>1122)
                     /locus_tag="IV329_002859"
     CDS             complement(713..>1122)
                     /locus_tag="IV329_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941955.1"
                     /translation="PQLEVELNASYFAADMIYTKVIISNYSTEPAMLVNLELSSANLN
                     RRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSAISCYLAFEVGKINFNKLLNHQ
                     TKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          392 a          158 c          231 g          341 t
ORIGIN      
        1 aaggagctat ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa
       61 gaagaatgac tttcaaagaa ctagctgcgc ttatcggtat ttcaggagct tatttatcag
      121 atattctaaa cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa
      181 aaatattgga catccgatag gggggtaggc gatgacaaaa ctaaaaaaac aagattttgt
      241 aaaaaaatac aattattctc catctactta tcaacgtcga atgtcggaac taaaaaatac
      301 agcaattttc tcagcggcgt atgaacgggt cacaggacaa gaagtttgga tcaatacaga
      361 attatacgat aaatttttgt ctttcaaatc ctataacagg ttacgcacaa gaaaggtaac
      421 acctaaagaa tttatcgaga agcatttagt tgatttataa aaaacagaac attttgagag
      481 gtgaaggtta atgggtaaat tcaacagagc attggtattc agcgcaccgc taatcatcta
      541 cgctttagga ctttggggaa gcaggcaagc gttgatagga acgattgttt acatggtttg
      601 gatttttatg gggcttgatg aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg
      661 ggctgactaa aaatgaatag aaaagagaaa ctagaatgta tattactatt actcagttta
      721 attctttcac taatttctct attgggaagt ttgtattttt gatatcaaca attttgtgga
      781 tttgggttct atttagagta aatatcattt ttgtttggtg attaaggagt ttattaaaat
      841 taattttccc tacttcaaat gcaaggtaac aagatatagc acttttagga ggaatattta
      901 gcggtacagc ttcagagtat attctattat catcgactga tcctccttta gcaattaatt
      961 tcttaaatgg agtagctgac catctgcggt ttaagttagc ggatgaaagt tctaaattaa
     1021 ctaacatagc tggttctgtt gaatagttag aaatgattac ttttgtataa atcatatctg
     1081 cagcgaagta agaagcatta agttcaactt ctagttgagg gc
//
LOCUS       DACDKL010000348         1121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.358, whole genome shotgun sequence.
ACCESSION   DACDKL010000348 DACDKL010000000
VERSION     DACDKL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.358"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1121
                     /locus_tag="IV329_002860"
     CDS             <1..>1121
                     /locus_tag="IV329_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8941956.1"
                     /translation="DTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKE
                     IERDFQIEFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFI
                     QIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYI
                     YDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGL
                     LNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLG
                     TSFKLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDV
                     EFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILK"
BASE COUNT          419 a          141 c          189 g          372 t
ORIGIN      
        1 aagacactga taagcagcag ctttttgaga aatatgatag atattttaga caaaagaatg
       61 ttgatatcca aaataattca gctttaacat atcccaccta tcaagagatt cgatttagac
      121 agtatgttaa ggaaatagag agagattttc aaatagaatt tgactattat tatcttcaaa
      181 taacgtctat ctttttgaat tcttggattt atcaaatcca attaacatcg gacgataaat
      241 tatattcctt catcaatgat attaatagtt ggtgtagtcg agaagttcaa ttagtcaatc
      301 gttttattca aataggggat atttatttaa caaaaattca aaaaaatgtt ttggttattt
      361 tatttaagtt aatgtgtagt aaagaatttc agcaaaaacg tttagcactg attttggctc
      421 atggagatct aacggcaacc tgtatagcaa gtactgctaa taggatcgtt gagagatata
      481 tatacgacgc cattgatatg ccactaatga cttcaagtag tgagattgca gacaaaatta
      541 atagatactt acaagagtgt accggatatg aagaaattat tttacttgtt gatatgggtt
      601 cactggagaa aatttatcta aatttggaac tagacgctca aaaaaaattc ggactactaa
      661 acaatgtttc aacaagaatg gctattgatg ttgcaaatca gatattatta gatttaccgc
      721 ttaaacagat tcttgaacaa gtaaaagaaa atcaaaatgt ggattatatt tatgaatcat
      781 caaatattaa gaaggatatg attgtctgtt cttgtgcaag cggattagga acttcgttta
      841 aattaaaaaa gatacttgaa gatagctttc caagggatac tgatcttgtc atagaaacgt
      901 gtgactatta ctctttagtg aatgaaaact atttatcgga gcttcgtaaa gagaacaata
      961 ttctttttat tgttggaaca ctaaatccac atctagaaga tgtagaattc ttttcaattg
     1021 aagatatgat tttaggaatg aatataagta aattctccaa tcaattgaat agacacttta
     1081 atgataatga actgaaagtt ttgaacaata atattctgaa g
//
LOCUS       DACDKL010000349         1116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.359, whole genome shotgun sequence.
ACCESSION   DACDKL010000349 DACDKL010000000
VERSION     DACDKL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.359"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IV329_002861"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IV329_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8941957.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DACDKL010000350         1115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.360, whole genome shotgun sequence.
ACCESSION   DACDKL010000350 DACDKL010000000
VERSION     DACDKL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.360"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            120..734
                     /locus_tag="IV329_002862"
     CDS             120..734
                     /locus_tag="IV329_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941958.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(899..>1115)
                     /locus_tag="IV329_002863"
     CDS             complement(899..>1115)
                     /locus_tag="IV329_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941959.1"
                     /translation="EESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYK
                     ELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          370 a          196 c          192 g          357 t
ORIGIN      
        1 ttttcttgat tgacatttat ttacaaaaaa tattgccatt taaaaagaaa agtgacatat
       61 ggcttcctcg tgtatgatta gggtgtattt taaatagttg cagaaagtag gtaaaagttt
      121 tgagagttgc atacatacgt gtctcatcga tcgatcaaaa tgaacaacgc cagattgaag
      181 aaatgaaaaa attcggtgca gagagaattt tcatcgagaa acaatctggc gcaacgatta
      241 ctcatcgacc tgttttccaa gaagcattag attacgtccg agatcaagat atttttattg
      301 tggaagctat tgatcgactt ggaagaaatt atgacgagat catcgcttca gtcaattact
      361 tgaagaagaa aaatgtgaag ctgatcatta ctagtttacc gatcatggct gaagcaattg
      421 gcaatcctct cctggataaa ttcattaagg atctgatcat ccaaattttg gccatgatcg
      481 ctgaacaaga acggactgaa tcgaaacgtc gccaggcaca aggaattaag atcgcaaaag
      541 caaacggcgt ctacaaaggg cggcccaaat tatacagtgc agacgcaaaa gatccccaac
      601 gtcgccttgt ttacaaaagc atcgttgaag atcttaaaaa tggtgttgcg attgctaaga
      661 ttgctaaaga ctacaatgtt acgagacaaa ccgtgtatag aataaaaaaa gacagcatgg
      721 tcaatgacaa atagttgatc atgctgcttt tattatcttc tcatctttaa tttcctacat
      781 ataattacta tctataaaat taagtcttac tcagaacaga taaagcataa aattccaaca
      841 aaccattttt tacttaacta aataaagagt ctgttaatat aatcttcact tatttacgtt
      901 aatcccctac tgtatttgtt ggttccttat ctatattttc tacaaaatcc ttattatctt
      961 gtgatttagc ttcgtataat tctttgtatg tcgacacatt ggttatacta attcctatag
     1021 ctagttcttg agactctact tttctctcta gctcctttga caaagtgatt tgagatttat
     1081 ttggttccgt tggagtcaat tctatagatt cttca
//
LOCUS       DACDKL010000351         1093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.361, whole genome shotgun sequence.
ACCESSION   DACDKL010000351 DACDKL010000000
VERSION     DACDKL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.361"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            289..951
                     /locus_tag="IV329_002864"
     CDS             289..951
                     /locus_tag="IV329_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8941960.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT          372 a          143 c          216 g          362 t
ORIGIN      
        1 tgattgtatc atcttttcat tttagaataa acctttatat gtaattttgc acttgacact
       61 tcagatggac gttttcttca aaataatatg ttttgaaact tgataagaga agccactgtc
      121 tttactaaaa agtaaaggct gcgataaagg acagtgactt taataaagtt gcactgttgt
      181 attgtctttg gataaaattg tctaaaggga agttttatcc ccaacgaaaa aacatctaaa
      241 gtggtaagta atgaaagaat gtcgataaag taggaatggg ggatcttttt gaatacaata
      301 gaatcacagt ttgataaggt tgcagaagat tacgatttcg tgaatgagct tttgaatgat
      361 tattcattct ttgtgtctaa tatgtctcca aagaaaggca gagcattaga tatcggatgt
      421 ggctcgggtt tgttagtgga gaaattagca agttattatg atgaagtggt agggattgat
      481 atttctaatc aaatgctcga tcttgccaaa tctaaacgtc aactaacaaa tacggtctat
      541 ctgaatatga atgcagaaca acttaatttt aatgagaagt ttgattttat tgtaagccga
      601 acaacctttc atcatttgga tgatatagcc agcgtaatac agcaaatgaa ggaactgttg
      661 aatgaggaag gaagaatagt tattcttgat aatgtatctg aagtcgagac accgcctacc
      721 tatgtttata aattaggggc aatccaagag tttttaccgc attgtttcaa atttggaata
      781 aaaaatgcga ttagaattta taatcacaac acatctaaat catggcttga gcatttagct
      841 tcagataagt acctatctga acaaaattat tacgatttat atgaaaagtt gttgcctgga
      901 tgccaattcc ataaaatggg ttgggcaatg ggggttgtct ggacaaaata acttaaagct
      961 gtggctttga aaagttgaat ttgtgattgt tttcagagct atcgggtgct ttagtataaa
     1021 taacaatgaa taaaaaatca gcctgtatct tatgttacta ttaagtaatt taagatacag
     1081 gttttactat ttt
//
LOCUS       DACDKL010000352         1087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.362, whole genome shotgun sequence.
ACCESSION   DACDKL010000352 DACDKL010000000
VERSION     DACDKL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.362"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..610
                     /locus_tag="IV329_002865"
     CDS             44..610
                     /locus_tag="IV329_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8941961.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            755..842
                     /locus_tag="IV329_002866"
     tRNA            755..842
                     /locus_tag="IV329_002866"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:787..789,aa:Ser,seq:cga)
BASE COUNT          360 a          177 c          219 g          331 t
ORIGIN      
        1 aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta
       61 taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg
      121 atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga tttatctgga
      181 attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg aacaactaaa
      241 agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg gtttgaacta
      301 tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg atgaacatgg
      361 ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag ttaaagaaga
      421 tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg gtacatgggt
      481 agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa cagaagaaca
      541 aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata atcctgaaat
      601 aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct gcaaactgtc
      661 aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa ataggcttga
      721 catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt ggtaatgcac
      781 cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc
      841 cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc
      901 aaacaaaaat agcttttcct attttttcgt ttgattgggc ttattaccgc agatgcaagc
      961 ttttgaacac cgtttgttcg gaataagggg ctgtgacaag acttttgtca cagcccctta
     1021 ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt ttttataaat
     1081 gctagta
//
LOCUS       DACDKL010000353         1080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.363, whole genome shotgun sequence.
ACCESSION   DACDKL010000353 DACDKL010000000
VERSION     DACDKL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.363"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..1042
                     /locus_tag="IV329_002867"
     CDS             44..1042
                     /locus_tag="IV329_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941962.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
BASE COUNT          305 a          168 c          172 g          435 t
ORIGIN      
        1 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
       61 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
      121 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
      181 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
      241 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
      301 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
      361 caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct
      421 aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag
      481 tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact
      541 attattttta acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc
      601 attacagaat ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt
      661 gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt
      721 gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa
      781 tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt
      841 ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa
      901 ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta
      961 tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa
     1021 tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta tctttttatt
//
LOCUS       DACDKL010000354         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.364, whole genome shotgun sequence.
ACCESSION   DACDKL010000354 DACDKL010000000
VERSION     DACDKL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.364"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IV329_002868"
     CDS             complement(55..789)
                     /locus_tag="IV329_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8941963.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          380 a          216 c          191 g          286 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa agg
//
LOCUS       DACDKL010000355         1069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.365, whole genome shotgun sequence.
ACCESSION   DACDKL010000355 DACDKL010000000
VERSION     DACDKL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.365"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..969
                     /locus_tag="IV329_002869"
     CDS             <1..969
                     /locus_tag="IV329_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8941964.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DACDKL010000356         1068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.366, whole genome shotgun sequence.
ACCESSION   DACDKL010000356 DACDKL010000000
VERSION     DACDKL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.366"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IV329_002870"
     CDS             complement(<1..276)
                     /locus_tag="IV329_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8941965.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            838..>1068
                     /locus_tag="IV329_002871"
     rRNA            838..>1068
                     /locus_tag="IV329_002871"
                     /product="16S ribosomal RNA"
BASE COUNT          351 a          193 c          202 g          322 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaataaac atttttaaca
      781 agcaagcaat atgctagcaa acaaattgag cttaacaatc gcaagattgt tcgaactttt
      841 tatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc
      901 gaacgcttct ttttccaccg gagcttgctc caccggaaaa agaggagtgg cgaacgggtg
      961 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata
     1021 ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcg
//
LOCUS       DACDKL010000357         1050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.367, whole genome shotgun sequence.
ACCESSION   DACDKL010000357 DACDKL010000000
VERSION     DACDKL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.367"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            411..1034
                     /locus_tag="IV329_002872"
     CDS             411..1034
                     /locus_tag="IV329_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8941966.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          399 a          141 c          187 g          323 t
ORIGIN      
        1 aatttgaaaa atatctaaca gatcttatag ataatagaaa taatgaatta atgagacaaa
       61 acattgcaag attccttact aaattagttt ctgaatggaa aagtattatt tctcaaaata
      121 tggataaatt aagatattaa ggatgttagt tttaaaataa actaatgtcc ttatttttgt
      181 gttcacattt ctgttaccta agaaatactt aaaggaggtg atgtagccag caacgactac
      241 aacttgaacc cgctatctac agtttatttg ttgtttcttt tgggtatact ctaatggtac
      301 aaatccatat ttgcttaaag cgttgatttt tcagcggttt ttctttattg caaagtgtac
      361 cctaagacct gtatactaat agtaaataga ttggatagga atgatggcaa atgtatttag
      421 cttatttaag agtttcttcc gacgatcaaa gtttagctcg gcaacataag ctaattgaaa
      481 attggtcagt caaaaataat attgctgaag acgatttaaa aattttcgag gaaaaagtct
      541 ctggaaaaaa tattgaaaac agaccagagc tgcaagagct tatttctttc atacgtgaac
      601 gagatacgat tattattcct agtcttgatc gattagcaag aaatagtaaa gatacaaaag
      661 acttacttca attgtttcgt agtaaaggtg cttctattga aatattagac ctaccgtcgt
      721 ttaatggggt aactgaccct gcattaagag acttgctaac aaatttagtc atagaagtgt
      781 ttagctatgt tgctgaaaac gaacggaaaa aaatcaaaga gcgtcaaaaa caaggcattg
      841 aaattgcaaa gaaaaatggt cgttatactg gtaaaaagat tcaataccat gccaatgcaa
      901 caggaaaaaa caaattgatt tacgatgaaa ttgttgatgg tctgaagaaa aaagaaacta
      961 tttctaaaat agcaaagaac gcaggagtta caagaaaaac cgtttatcga gtaaaaagag
     1021 atttaggaat gtaatatgtt ccagttagaa
//
LOCUS       DACDKL010000358         1045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.368, whole genome shotgun sequence.
ACCESSION   DACDKL010000358 DACDKL010000000
VERSION     DACDKL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.368"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..307
                     /locus_tag="IV329_002873"
     CDS             68..307
                     /locus_tag="IV329_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941967.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(669..929)
                     /locus_tag="IV329_002874"
     CDS             complement(669..929)
                     /locus_tag="IV329_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941968.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          342 a          188 c          147 g          368 t
ORIGIN      
        1 tgcattgatt ttgcctaata ctgagcatag gtgtatgcta aacgatacta taaggagtgt
       61 gtacataatg aaaagaattc tgtttatcag tttatcaaat ttattgttac ttactgcttg
      121 tgggacttct gcatcaaaga acataccttc agatacgcaa atttcgaata gtatcgattc
      181 aacaatttct acaaatgaga aaaattctcc ggtagaaact tccgatataa aagtttctac
      241 ctctgcaaaa aacagggtaa agaaactgtc agcacgtctc ctttatttca aacaaactct
      301 gaattagctt cttcatcttc ttcagcaaat aatgatgata gtttcgcgtt tactcctaac
      361 taaatgcaac ctttacaaat gctttacgat aacaagcctg agctaaatga tcctgatatc
      421 agttttgcat tttttgaaat gattggatcg gatgatcttt ttaaaactac tagtttgtct
      481 atccgaaaat aggacagttc tggcagtata ggtttttatc gagtttctcc accaggcctc
      541 gttacagaga cagattctct aggcgaacct tttgaataaa tcaaaaatca tgaacaatca
      601 ctgccaacta aataaagtgc cttcaaaagc ttggtcttct cgcttttgaa ggcactttat
      661 ttaaatcttt atgaaactaa agtatgttca tatttgttgc tatagttata tagatattct
      721 gttgtttcca actcataaac gtgagtatat tttgccacta cttttccgca tttttctaat
      781 atttcattgc gatcgacgtc atgatattcg tctacgcaga tcacaacaca attttctaat
      841 ttactgatta cttctccaac cactttttgt ttcaagccaa taggttggca aatatatttt
      901 tttcctaaaa tgatagtttc gttattcata tttcatccat ccttaatgtt ttttcattat
      961 tcaaaatgat aataccatta aaatatgatt gtgaaaatca ataacacttg tgattttata
     1021 aaaaaataaa aaaaccgaaa aacag
//
LOCUS       DACDKL010000359         1037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.369, whole genome shotgun sequence.
ACCESSION   DACDKL010000359 DACDKL010000000
VERSION     DACDKL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.369"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            103..1037
                     /locus_tag="IV329_002875"
                     /pseudo
     CDS             103..1037
                     /locus_tag="IV329_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
BASE COUNT          359 a          157 c          181 g          340 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaa
//
LOCUS       DACDKL010000360         1026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.370, whole genome shotgun sequence.
ACCESSION   DACDKL010000360 DACDKL010000000
VERSION     DACDKL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.370"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..390
                     /locus_tag="IV329_002876"
     CDS             40..390
                     /locus_tag="IV329_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8941969.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            489..1019
                     /locus_tag="IV329_002877"
     CDS             489..1019
                     /locus_tag="IV329_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941970.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
BASE COUNT          303 a          163 c          198 g          362 t
ORIGIN      
        1 aagtagtaaa taatttgaaa aaaagaaagg tgactgatta tggcgcaaaa tcgtattttt
       61 acgacaagtt ttgcaagcgt ttatcctatg tatgtagcaa aagcggagag aaaagatcgt
      121 ataaaagagg aagtagatga aatcatttgt tggctgacgg gttattccca aacagaacta
      181 gaatatcaac tacaaaaaca agtgactttc gaacaatttt ttgcggaagc ccctgtcccc
      241 aatccttcac gttccttgat tacaggtgtt gtttgtggtg tacgagtaga agacatcgag
      301 gataacttga tgaaagaaat tcgttattta gataaaatga tcgatgagct agcaaaaggc
      361 aaaaaaatgg aaaagatcct cagaagttga tttttccaga gatattcttc tccttcgctc
      421 attttgtaac atttaatttg gtaaaatgac tagttcacgt taaaataagt caaaggaggt
      481 tttcttatat gtttgagacg attaaacaaa atattcaaaa atacgcctta ttaagagctg
      541 ctgtttatat tatttctggg attgcgattg tgctaaaccc ttctgctgtt tttcatttta
      601 tcggttatct gatcaccgcc tatcttgttt tgcttgggtt gttgaatctt tttgaggctt
      661 accgttttaa aaggaaaaca ggttcttgga gtatcggtct gttcagtggt ctttttttcc
      721 tgatcttagc tctgatcgtc tatgcttttg cacctgcaat cgtttctatc ctgccgattc
      781 ttcttggatt agctgttatc atcaacagct tgttccagtt gttcttcagc atgaatacaa
      841 aagctaaaag ttggagtgtt tatagtagta ttctgttgtt gattggcgga ttcgtcttat
      901 tgttcaatcc attcaaaagt ttgatgcttc tattccaaat attcggcttt attttgattt
      961 tcatgggcat ccaggagatt atcaatttca tcagaataag aaaaatgact atacgttaat
     1021 catttt
//
LOCUS       DACDKL010000361         1012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.371, whole genome shotgun sequence.
ACCESSION   DACDKL010000361 DACDKL010000000
VERSION     DACDKL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.371"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..>1012
                     /locus_tag="IV329_002878"
     CDS             75..>1012
                     /locus_tag="IV329_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8941971.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYD"
BASE COUNT          388 a          131 c          175 g          318 t
ORIGIN      
        1 aaagatgagg atgttgtgta tctaatcaat cagatagaaa attacacaaa aatactttat
       61 gctgaggttt caaaatgaca aatatcttcg aaaaaatcgt aaatgattta aatttagaac
      121 aaaaagatgt tgtggaaagt gaagataacc tttatgtaaa tgcatgtcct gggagtggga
      181 aaacaagggt acttacaagg aaaattgctt accaagcaat taaacacgat ggatcactaa
      241 aaagaataat tgctatcaca tacactaata gagctgctga agaaattaag gaacgcctaa
      301 gtttacttag tatagatgat gatattaata tttgggtggg gacaatacat cagttttgtc
      361 ttgaatttat tatttatcct tttaaaatga atctctcaag agtatcaaaa ggatttacaa
      421 ttattgatga ttatactcaa agagcttacg tcagccaaat taataatatg ctgaatttaa
      481 atctcaaaac gtatgaactg aacaaaattg atttgtcact ttctcatgat tttaaaatta
      541 atgaagttca gtatactcaa gtttctgaaa tgtatcacac tatgttggaa aaaaataaag
      601 aaattgattt tgatatgatt ttactagtag cgtataaaat tttaactgta aacagtaacg
      661 ttgcacgtaa tattgctagt aatataaggg cgatttatgt ggatgaattt caagatacta
      721 gagaattaca atacaatgta atagctaaat tacttcaaaa caaccctcaa attcagtcaa
      781 tgtttgttgg agatattgac caagcaattt atggatcatt agatggtata gctaaatcag
      841 ttgaagaact agaaatgcta actagtcaca cttttcaatc aaagacgcta catggctgtt
      901 acaggtctaa tcagagatta gtagacttct actctaattt tcagagttgt ccttacgaga
      961 ttgaaagcag agggaataat aaaaacgacc gtggttttat aagttatgat tg
//
LOCUS       DACDKL010000362         1006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.372, whole genome shotgun sequence.
ACCESSION   DACDKL010000362 DACDKL010000000
VERSION     DACDKL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.372"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..812
                     /locus_tag="IV329_002879"
     CDS             <1..812
                     /locus_tag="IV329_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8941972.1"
                     /translation="YFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLT
                     KLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISMS
                     TFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEK
                     RVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTN
                     NVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            845..>1006
                     /locus_tag="IV329_002880"
     CDS             845..>1006
                     /locus_tag="IV329_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8941973.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEF"
BASE COUNT          390 a          111 c          189 g          316 t
ORIGIN      
        1 tttattttgg cgggggaatt aatttatggt tagccaaaaa gaatggaatc agacagagaa
       61 ttgctcatag ccatgcaact gaagatggaa aatctcaaaa tattgtaatg aatgtattgc
      121 gtaaattttt aaccaaattg ttgttacaaa atgcaacaga ctaccttgcc gttagtcaag
      181 aagctggtga aagtctgttt caaaaccatc cctttgagat agttcataat ggtattgatt
      241 tagagcttta tagtaaaaat tcagaagcta aggttaataa gagaaaagaa ttggatatct
      301 caatgagtac ttttgttgtg ggaaacattg gacgattaga gaaacaaaaa aatcaatctt
      361 atttgttaga gattttttta gaaatactaa aaatgaattc tgattcttac ttgattttag
      421 taggtgaagg gtcacttaga aataaactga aagccaaagc agtagaattg gatatagaga
      481 aaagagtaaa atttttagga gaaagaaacg atattcccga gttattatct gtgatggatg
      541 tttttgtaat gccttctctt tatgaaggtc tgccaatggt tggattagaa gcacaagcag
      601 caaaattaaa attagtctta gcagatacta tttccaaaga tacaaagtta acaaataatg
      661 tggattttgt atcattagat gaaacaccac aagaatgggc aaaaaaagta ataaaagagc
      721 cgtatgctaa taaaataaca caagaactaa agagttatga tgtttcgtac actactgaac
      781 aaatggagag tatatatagt agagatcatt aaaaatgttc aagatatttt aggagtgatt
      841 atttttgaaa atatgttatt tgatattggc atacaatgat attggcaact taaagagatt
      901 ggtcaatcga ttgaacgaaa atgcagtttt tattattcat atagataaaa aaactgatat
      961 agaaccattt attgaagcat ttaaatcata tgataatgtt gaattt
//
LOCUS       DACDKL010000363          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.373, whole genome shotgun sequence.
ACCESSION   DACDKL010000363 DACDKL010000000
VERSION     DACDKL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.373"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IV329_002881"
     CDS             complement(26..913)
                     /locus_tag="IV329_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8941974.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DACDKL010000364          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.374, whole genome shotgun sequence.
ACCESSION   DACDKL010000364 DACDKL010000000
VERSION     DACDKL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.374"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IV329_002882"
     rRNA            complement(<1..>994)
                     /locus_tag="IV329_002882"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDKL010000365          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.375, whole genome shotgun sequence.
ACCESSION   DACDKL010000365 DACDKL010000000
VERSION     DACDKL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.375"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..988
                     /locus_tag="IV329_002883"
     CDS             <1..988
                     /locus_tag="IV329_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8941975.1"
                     /translation="SSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHS
                     ITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTY
                     LEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFG
                     TKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCM
                     LVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDY
                     PIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT          373 a          150 c          186 g          285 t
ORIGIN      
        1 atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac
       61 gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa
      121 acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat
      181 aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc
      241 gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa
      301 cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg
      361 gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc
      421 agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg
      481 gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat
      541 atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc
      601 agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt
      661 cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg
      721 taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa
      781 atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa
      841 aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa
      901 tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat
      961 attggaaaaa ttggatttcg ttaattaaga aaag
//
LOCUS       DACDKL010000366          992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.376, whole genome shotgun sequence.
ACCESSION   DACDKL010000366 DACDKL010000000
VERSION     DACDKL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.376"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..810
                     /locus_tag="IV329_002884"
     CDS             <1..810
                     /locus_tag="IV329_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8941976.1"
                     /translation="NDFSPKEQAIADYILENPSKVSHSPISNLASELGIADSTFFQFT
                     KKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESSISTLNDTKKL
                     LQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHSTDYHIQLMEA
                     SLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAKLGDVVFISIS
                     EETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISEIKE"
BASE COUNT          332 a          179 c          176 g          305 t
ORIGIN      
        1 aacgacttca gcccaaaaga acaggctatc gccgattata ttttagaaaa tccaagcaaa
       61 gtaagtcata gtcctattag caatttagca agtgaactgg gcattgctga ctctactttt
      121 tttcaattta caaaaaaact tggttataat ggatttaaag attttaaaat ggccatgttg
      181 atccaagaaa atgatctatc tgctatttct atccacgaaa atattcagaa aacagatact
      241 gagctcacta tggcgcaaaa ggtgttcgag tctagtattt caactttaaa tgacacaaaa
      301 aagttgttac aacaaaagga tctaaaaaaa gcggctacta ttatcaatga atctaaacgt
      361 gtctattttt tcggtgttgg cggttcagag attgtagcaa ctgacgctta tcataaattt
      421 ttgcgttccc ctatcgcaac ttctcacagc acggattacc atattcaatt gatggaagct
      481 tctttattga cggaaaaaga ctgtgctgtt ttgatttctc acacagggca atcaaaagaa
      541 acaatccata ttgctgaaac cgtaagaaaa actggagcaa aaacaatcgt tattactagc
      601 caagctaatt cttcccttgc taaacttggc gatgtcgttt ttatttctat ttctgaagaa
      661 acagaattcc gatctgaagc tttagcttcc cgtatttctc aactaagcat tttggactct
      721 ctatatgtga ttttgatgtt ttacaacgaa aaagaatcac gtcgttctat cgctaaagtt
      781 cgacatacta tttctgaaat caaagaatag caccataaca gaaaaagcga aaaatccata
      841 aaacaaaaaa agccactacc aaatatttgg tagtggcttt tttggttctg caagggtggt
      901 acagaacatc aatagtcggt gggttagaac gtatgtcatc gcatctttgg agaaggttgt
      961 attggtatta ttcgagttac ttgttagatg cg
//
LOCUS       DACDKL010000367          986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.377, whole genome shotgun sequence.
ACCESSION   DACDKL010000367 DACDKL010000000
VERSION     DACDKL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.377"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IV329_002885"
     CDS             <1..704
                     /locus_tag="IV329_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8941977.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DACDKL010000368          978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.378, whole genome shotgun sequence.
ACCESSION   DACDKL010000368 DACDKL010000000
VERSION     DACDKL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.378"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..>978
                     /locus_tag="IV329_002886"
     CDS             25..>978
                     /locus_tag="IV329_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8941978.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFN"
BASE COUNT          335 a          133 c          202 g          308 t
ORIGIN      
        1 actaataagt agaggagaaa gaaaatgact ataaaagtag acttacgaaa aaaaccatat
       61 catttagatg aagatgctat ttcttggatt gaagagacga ttgatgctat gactttagaa
      121 gaaaaaatag gtcaattatt tgttaatatg ggatctagtc gtgataaaaa ttacttaagt
      181 agtatggtca atcagtacca tattggagct gtacgatata atccaggaac tgctgatgag
      241 gtatatgatc aaaactatat cctacaaaca gaaagcaaaa ttcctttatt aattgctgct
      301 aatacagaag caggtggtaa cggagcttgt acagatggta ccgaagttgg tgtagaagta
      361 aaaattggtg caacaaatga tgcgaaatat gcttatgaat tagggagagt ttctggaatt
      421 gaagcatcgg ctattggatg taactggagt tttgctccta ttgtagatat taatagaaat
      481 tggagaaatc ctatcatttc tagtcgatct tttggacaaa gccctgataa agttttagaa
      541 cttagtttag cttatatgaa aggtattcaa gagagtggaa ttttgcctgc agctaaacat
      601 ttccctggtg acggtattga cgaacgagat caacatctat cttttagtat taatagttta
      661 agtacagaag aatgggataa ttcttttgga aaagtatact caggcttaat agaagctgga
      721 ttaccttcaa ttatggcagg tcatatccat atgccagctt attcaaaaca ttttaatctt
      781 gatctgaaag atgaagattt acttcctgca actttatcaa aagaattaat cactgactta
      841 cttagaggaa aactcgggtt taatggatta gtagttactg atgctagtca tatgttagga
      901 atgactgggg caatgaaacg gtcagaatta ttgcccactt ctattgcggc tggatgtgat
      961 ctattcctat tttttaat
//
LOCUS       DACDKL010000369          967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.379, whole genome shotgun sequence.
ACCESSION   DACDKL010000369 DACDKL010000000
VERSION     DACDKL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.379"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>967
                     /locus_tag="IV329_002887"
     CDS             <1..>967
                     /locus_tag="IV329_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8941979.1"
                     /translation="LVILSMSTLSLISLSIKDATDRASKETFANITNSFSMEINRQVN
                     PGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAK
                     NFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDK
                     IKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVY
                     GNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGI
                     YSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLE"
BASE COUNT          402 a          108 c          169 g          288 t
ORIGIN      
        1 attagttatt ttatctatgt caactttaag tctcattagt ttatctatta aagatgctac
       61 ggacagagct tcaaaagaaa catttgctaa tataacaaat agtttttcta tggagataaa
      121 tagacaagta aatccaggaa cacctagagg tgggggaaat gtaaaaggtg aagatattaa
      181 aaagatatct caaacagata gtatagactc ctatgtaaaa agaataaaca gtgttgcaga
      241 tttagttgat tatgatatta ttgaaactca agagactctt gcgaatcaat cacctgaaag
      301 agcaaagaat tttaaaagaa cagttatgtt aactggggtt aacgactcaa caaaagaaac
      361 aaaatttgta tcagaagcat ataaattagt agaaggaaag catttagaaa atgaagataa
      421 aaataaaatc ttaatgcata aagatttagc tgaaaaaaat aaccttaaag ttggagataa
      481 gataaaaata aaatctaatt tatttgatgc tgataatgag aaaggtgcag atgaaacagt
      541 agaagtagaa attaaaggtc tattcgatgg gcataatagc ggtggagtaa gtgcagcaca
      601 agaactatac gaaaatacat taatcactga tattcataca gctgctaaag tttatggtaa
      661 taccgaagat acagctgtat accaagatgc aacattcttt gtaaaaggtg ataagaatct
      721 tgatagtgta ataaaagatc ttgggaaatt agatataaat tggagagaat ataatttgat
      781 taaaagttca tcaaattacc ctgcattaca acaatctata tcaggtattt actcaatttc
      841 aaataaatta ttttttggct cattaatatt tgcaggagtt gtagtttcat tattattatt
      901 cttatggatg aatgctagaa agaaagaaat agcagtatta ctatcattag gtatatcaaa
      961 attagaa
//
LOCUS       DACDKL010000370          961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.380, whole genome shotgun sequence.
ACCESSION   DACDKL010000370 DACDKL010000000
VERSION     DACDKL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.380"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..>961
                     /locus_tag="IV329_002888"
     CDS             6..>961
                     /locus_tag="IV329_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8941980.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIK"
BASE COUNT          377 a          138 c          167 g          279 t
ORIGIN      
        1 agataatggg catcgaattt agcgaacggc aaatgaaaat actatacttt ttgattgaaa
       61 atgagcacac aacttacaaa gaattatcag aacaattata tgtatctacg aaaacaatta
      121 gcaaagatat tttaattttg aaagaaataa tccaaaaaat gccagatgta caaatgttgg
      181 ttcaacctaa aaaaggcgtt tcgcttattg gaaaaaaaga aacacttcga aatttattat
      241 ccagtcataa aatcaatatc gcagactctc cggaggaccg tctaacttat gtgtttgcta
      301 aattattgaa tactaatgat tctctaaaga ttcaaaatct tgctgacgaa ttatacgtga
      361 gcaggtcgac ggtagaaagc acaatgaaag atgttagaaa aagactaaac aagcatggga
      421 tgtccttagt ttcagatcga aatggcgtgc gcatcaaagc tgatgaaaaa agtaaaagaa
      481 ggttgatgtc agaagttgtg aactattatt ggggtggctt tacagcatct aacagtaaaa
      541 cagatctctt agacttgaat atcaatttca ttggtgaagc gaataatttc ttaaataagg
      601 acatattgaa aaaagtagcc gaattgctta atcgatacat gaataagaca caattgaaat
      661 ttacagatta tgaatttcaa actttagcta tccaccttgc tattgcgata gaaaggattg
      721 aaacaagcaa tttcttatca cgtagtaata attctgaaga agaaatctca aagaatacac
      781 ttattttaat caaaatgatt gaaaaagcat tttcaattat tgtgccaata gatgaacaag
      841 cttatgttga cattcacatt acttcgattg aaaaaaatgc tatgaatcac gacttagatc
      901 aaacgaagct ttcctattta gatatgggtg ttgagctaca agagttagta agaattaaat
      961 t
//
LOCUS       DACDKL010000371          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.381, whole genome shotgun sequence.
ACCESSION   DACDKL010000371 DACDKL010000000
VERSION     DACDKL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.381"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IV329_002889"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IV329_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="IV329_002890"
     CDS             complement(570..>959)
                     /locus_tag="IV329_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8941981.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DACDKL010000372          950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.382, whole genome shotgun sequence.
ACCESSION   DACDKL010000372 DACDKL010000000
VERSION     DACDKL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.382"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..775
                     /locus_tag="IV329_002891"
     CDS             <1..775
                     /locus_tag="IV329_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8941982.1"
                     /translation="TQVQLAEGICEQATVSNIENKNMSPGINILSALCKKLNVEISDI
                     LVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKVSNSDLLNQILFYQGLLDH
                     LYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKIL
                     SETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIA
                     SCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT          353 a          134 c          182 g          281 t
ORIGIN      
        1 aacacaagta caattagcag aaggcatttg tgaacaagca actgtcagca atatcgaaaa
       61 caagaatatg tcgccaggca tcaatatctt aagtgcgctt tgcaaaaagc tgaatgtcga
      121 gatctccgat atattagtag aaacagaaga agacaaaatc aggaaaaaaa taaatgagat
      181 tcaatcattg aattctgtat caaaatacca agaatcttat aaaattttac aaacgatcga
      241 tcaggaaaaa gtaagtaatt ctgatctttt aaatcaaatt ttattttatc aaggactgct
      301 tgatcatctt tatgcgaaaa aaccagatgc agcattattt tattttaatc aagtgttgga
      361 acaaacgacc gaaacagata tccatcactt acaagcagct gcaaacgtgg caatgatcta
      421 cttgtcaaaa ggagaattag attttgcgaa agtttatgtg gagcgtacac ttaaaatatt
      481 gagtgagact gattttgata atctgatggt ctgcattgta tattatgata tcgccaccta
      541 ttaccgtaaa atcgaggatt atgataaagc aatccaatta tgtgagaagg gtattgagta
      601 taataagaaa cataaatcga cttatgcatt ggaatacttg ctttatgaaa ttgcttcttg
      661 ccataagcaa ttaggagaag atgattattt agaaagatat atggacgcaa aaaaaatagc
      721 gcgtttcaac ggaaatgatt acgcggtaaa ggtgattgaa aacgacctga aataaagtgg
      781 ttgtgaaatc agtgttctga tttgaatacc gtttattcga aaaaaggagg tgtgactttt
      841 gtcatacctc cttttttcga tattgtttga ttcctattta ggcaacatat aacggttata
      901 aaagaagcaa tagattatga tggtatcgat tggatggata catcttggga
//
LOCUS       DACDKL010000373          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.383, whole genome shotgun sequence.
ACCESSION   DACDKL010000373 DACDKL010000000
VERSION     DACDKL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.383"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..299
                     /locus_tag="IV329_002892"
     CDS             66..299
                     /locus_tag="IV329_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8941983.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            296..703
                     /locus_tag="IV329_002893"
     CDS             296..703
                     /locus_tag="IV329_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8941984.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          350 a          133 c          157 g          303 t
ORIGIN      
        1 tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa aggagggaga
       61 agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta gtattaacta
      121 tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt tttttagatg
      181 aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa attgatttag
      241 caatatctca agcattaact gaatatgata ctacttttag agagcttgtc gataaatgag
      301 tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa taaaaaaata
      361 ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt gtgtagggca
      421 acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa aagcagctat
      481 cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac gtactgcttt
      541 cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg atcaaaaaga
      601 tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc aactaaaata
      661 tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta ctgtatgtgt
      721 ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa atagctataa
      781 ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa gataacgact
      841 atttatttta tcatattttt actataccct atacttttac ttggtatatt tatgaataat
      901 cgcaaattta cccctatact actagtttgt aagttagatt aat
//
LOCUS       DACDKL010000374          934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.384, whole genome shotgun sequence.
ACCESSION   DACDKL010000374 DACDKL010000000
VERSION     DACDKL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.384"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..661)
                     /locus_tag="IV329_002894"
     CDS             complement(83..661)
                     /locus_tag="IV329_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8941985.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT          295 a          173 c          161 g          305 t
ORIGIN      
        1 ttgaatacta gtatcactcg gatatttgat cgtttattta ttttttctat atttttctct
       61 taaattttaa acacggatca ttttaccgat attgtgattc gatggtacga cttgggatga
      121 tccaagcagc tagaatcacg atgtttgcga tcagcacggc tactggcgct actaaatacc
      181 ctaagacaat tcctaacaca ttcaaaccaa tcgatagata cgatttcttg taattattca
      241 acatcgcctt caatgctttc tcgtggcctt tatttgcttt gacaagttct tttgccaaga
      301 tataatacgc aatattagcg gtcaaggtaa caaaaccata agtcatttct ggagccaggc
      361 tattgatgta ctcactgatc catgaagttg caaacggaaa aagcgaaagc gcaaaaacaa
      421 agaaactgtt cgcccataag acccgtccat ttatcttatg tacaacttga aataaatgat
      481 gatgattgtt ccaataaatc gctaaaatga taaaactgac aatatagata aaaaatcggt
      541 aacctaatgc agcgagcgcc tcccacgtgt ctccgttagg ttcatgaagt tctaaaacta
      601 aaatagtcat gataatcgcg attacagcat ctgtaaatgc ttcaagacga tttttaggca
      661 tgatattctc ctccttttcc gctctatttt agatgataaa ccagagaaaa agcaattgtt
      721 tgatcattta agtagatgaa gtaaaaatca gtcttcagtt ggaggtgttt ttaaacaatc
      781 cctgttatac tttttctata gtaaagaatg aacaagagga gggttttgta tgcgtcaaaa
      841 aaatatgttc ttaaatatct tcatggttct tttagtgcta attgcagcta gcttcgtcat
      901 cagtcttttt gtcagtgtcc taagcgggct ttta
//
LOCUS       DACDKL010000375          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.385, whole genome shotgun sequence.
ACCESSION   DACDKL010000375 DACDKL010000000
VERSION     DACDKL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.385"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..494
                     /locus_tag="IV329_002895"
                     /pseudo
     CDS             1..494
                     /locus_tag="IV329_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            607..>927
                     /locus_tag="IV329_002896"
     CDS             607..>927
                     /locus_tag="IV329_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8941986.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT          348 a          151 c          160 g          268 t
ORIGIN      
        1 atgaagtggt cactattact ccaagaaatt atcgtttcga ttttagaaag gtaaaaaatt
       61 tagataaaca ttatgcaatg ataggaatag cagctggggt atccaactat ccactctatt
      121 atgaggctac aaatgaaaaa gggttgagta tggcaggact gaattttcct ggcaatgctg
      181 attataaaga attacaagaa ggaaaagata acgttgcacc atttgagttt attccatgga
      241 tactagggca atgctcaacc atagacgaag ctaaagaatt attggctact atcaatctag
      301 tcaatatcga ttttagtgaa aaactaccct tgtcaccttt acactggtta ttagctgata
      361 aagaaaaatc gatagtaatt gaaagtatga aagatggact tcatttatat gataatcctg
      421 tcggtgtgtt gacaaataat ccgccatttg attatcaatt atttaatctg aacaattatc
      481 gctcgctatc aaatggaacg agagtataaa atattttgtg taaatgaaaa aatccataca
      541 aaaaaggaag tcccttctgt agaataaagt taacgacaac caattcacag aaaagaggac
      601 ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa aggcgattta
      661 aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg gactgaatta
      721 acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg taattcgaga
      781 aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt ggaaatacct
      841 agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag aacaaacgat
      901 acattggaaa ccactattat ccattta
//
LOCUS       DACDKL010000376          924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.386, whole genome shotgun sequence.
ACCESSION   DACDKL010000376 DACDKL010000000
VERSION     DACDKL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.386"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            199..834
                     /locus_tag="IV329_002897"
                     /pseudo
     CDS             199..834
                     /locus_tag="IV329_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          308 a          169 c          172 g          275 t
ORIGIN      
        1 agcaagctag tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa
       61 tcattatgga tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat
      121 tcgcaaaata taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc
      181 cattgttatt ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag
      241 ccgtcaaaaa ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca
      301 attgtggata aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt
      361 ttgcctacac gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa
      421 gcggtggaat atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc
      481 ttaccaaatg cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg
      541 ttcaatgatt ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa
      601 gccgagaaag tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac
      661 tatgattaca aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca
      721 atgatgattg atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc
      781 catgagctga ccgaagcctt tagagacaag gaatcagact tattttttac cttatattat
      841 ttgtcaaatt aagcgagaca aaactttcca tttacgtaac tcaaaaaaac ttctaatggt
      901 gtttggtaat ttagtgattt tcta
//
LOCUS       DACDKL010000377          920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.387, whole genome shotgun sequence.
ACCESSION   DACDKL010000377 DACDKL010000000
VERSION     DACDKL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.387"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..891
                     /locus_tag="IV329_002898"
     CDS             <1..891
                     /locus_tag="IV329_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8941987.1"
                     /translation="NGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREG
                     IVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKELIERDKNHPSVV
                     MWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDICKVGDIVDVL
                     CLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGLHDTVPTM
                     FTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNKKGIFTRDRKPK
                     MIAHYLKKRWSSIPNFSYKK"
BASE COUNT          342 a          121 c          193 g          264 t
ORIGIN      
        1 aatggtcgtg gtttgaatga agcagtaaat atcaaagatt ttaatttaat gaaatggatc
       61 ggtgctaatt catttaggac ttcacattat ccttattcag aagaaattat gcgcttagca
      121 gatcgagaag gtattgtagt tattgatgaa gtacctgcag taggattaca tttgaatttc
      181 atggctactt tattagatga caacgtagaa aaacatgata cttggaaaga aatacggaca
      241 gaaaatgctc acaaaatggc gattaaggaa ttgatagaac gcgacaagaa ccatccgagt
      301 gtcgtaatgt ggtctattgc aaatgaacca gattcggatt ctgaaggcgc taaagaatat
      361 tttgaacctt tagttgaatt gactaaagaa cttgaccctc agaagagacc tgtaacaatc
      421 gttacttact taaagtcaac accagatatt tgtaaagtag gagatattgt cgatgtttta
      481 tgtttaaatc gctattatgg ttggtatgtt tcaggtggag acctaaaaca tgcaaaatat
      541 ctgttgaaaa aagagctaga tggatggatg gaaagatgtc ccgatacgcc aataatgttt
      601 acagaatatg gagcagatac agtagctgga cttcatgata cagtaccaac aatgtttaca
      661 gaagagtatc aagtgggtta ttacgaagca aatcacgaag taatagataa atgtgacaat
      721 tttgttggtg aacaagtgtg gaattttgca gattttgaaa caagtcaagg gattttaaga
      781 gtgcaaggaa ataagaaagg aatctttact agagatagaa agcctaaaat gattgctcac
      841 tatctaaaga aacgatggtc atcaatacca aatttcagtt ataaaaaata atttaccttt
      901 tataaggaat ataaaaaagg
//
LOCUS       DACDKL010000378          915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.389, whole genome shotgun sequence.
ACCESSION   DACDKL010000378 DACDKL010000000
VERSION     DACDKL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.389"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            110..>915
                     /locus_tag="IV329_002899"
     CDS             110..>915
                     /locus_tag="IV329_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8941988.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTII"
BASE COUNT          329 a          176 c          129 g          281 t
ORIGIN      
        1 tcattttatt taaatttatg cagttatatt ctatatctta caaacacatt ccatgtattc
       61 caattcccta tccgttataa taaaagaaac aggcaacagg aggaaaatca tgatcaaata
      121 ttttacatta gataatgaga aactgcgtaa taccggaaag gaaactgacc aaacgatctg
      181 gtattgtgta gaacgtccta atcaagaaga gattcatcag ctgacacatc aatttgaaat
      241 tccgtcagat tacatcactt ccatattgga cgatgcagaa aattcccgta cagaagaatt
      301 caatcaatca aagttgacaa agccagcgtt attattgtta caatatcctt ttgctaaaac
      361 aagtcctagc ggttattttc aagtggatac atacccgctc tctattattt tgacgcccaa
      421 aaagaagctt atcacaatta cgaatcatga accactgttt ttaaagaaag tctttagcca
      481 gacctttcca aaaagtgata tttcgcctaa tctcaatatc ttctttcaat tcatttggca
      541 ggtcacactc tcttacaaca gttatttagc tacgcttcaa tctcaatgcg atactttgga
      601 agccaaactt caagtatcca ctgaaaacaa acaactttat caaatcatgg atatccaaaa
      661 aagcctcgtt tatttcagag aagcgacttc tgctaattta gatgcattga aagaactagc
      721 gcaaaataaa cttttccaag aaaatcatgc tttgaaaaat catttacgtg atgtattggt
      781 ggaaacagag caggcaatga ctacttctcg cattcaatta aaactagtag aacaaatgaa
      841 tcagactttt tcagcaatcg tatcgaataa tctgaataat attatgaaaa ttttaacttc
      901 tttaacgatt atttt
//
LOCUS       DACDKL010000379          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.390, whole genome shotgun sequence.
ACCESSION   DACDKL010000379 DACDKL010000000
VERSION     DACDKL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.390"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            118..>910
                     /locus_tag="IV329_002900"
     CDS             118..>910
                     /locus_tag="IV329_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8941989.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLS"
BASE COUNT          301 a          134 c          215 g          260 t
ORIGIN      
        1 ttttccgatg cagatgtttt tcattttttc tttcggatac cttgacatat gaataaccat
       61 tcatttataa ttagttatga agagatgttc atatgttgag gcgaggaggt cattaaaatg
      121 aaaagttaca gattagaagg tttagattgt gcgaactgtg caatgaaaat cgaaaaagga
      181 gttcagaaga tcaatggagt caaggaagca acggtaaact tcacttctgg aaaactaacg
      241 attgacgcag aggaagatca tttggctacg attgaacaag aaacaaaaaa agtagtgaag
      301 gaattagaac cagatgtcaa agtaacagaa atagataagg aaaaagtatc tgaacatgga
      361 aatgaaaaag agagaaatac gctgttccgg attttatttt cccttgcagg tatcgcttta
      421 ttgctattat ttgattttaa tgaaccaatt cgcttgatag ggtatttatt gatttatctg
      481 ctgataggat atgatgtagt gaagaaggca gtgatgaata tcgtcaaagg aaaaatcttt
      541 gatgagaatt ttcttatgtc tgttgctacg attggcgcta tgttgatcgg tgaatatcca
      601 gaagccgtag cagtgatgct attttatcaa atcggcgagt atttccaagg attggcagtc
      661 agtcattcaa gaaaatcgat tcgcgaattg atggcgatcc gtccagaaac agctcacgtg
      721 cagacgacgg aaggattgat gaccgtgaac cctgaagacg tgcagatcgg tcagtttgta
      781 cttgtgaagc ctggtgaacg tgtaccgtta gatggaacga tcatcgaagg agaatcatta
      841 gtcgatacgt cagcacttac aggagaatca gtccctaaat ctgtatatgt tggtgaaaca
      901 gtattaagcg
//
LOCUS       DACDKL010000380          905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.391, whole genome shotgun sequence.
ACCESSION   DACDKL010000380 DACDKL010000000
VERSION     DACDKL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.391"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>905
                     /locus_tag="IV329_002901"
     CDS             <1..>905
                     /locus_tag="IV329_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8941990.1"
                     /translation="EIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNELTDDEL
                     LELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVA
                     KELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTS
                     FNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRN
                     EDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKY
                     WRAGLYKVFSLDLVQDIATQVSSIQ"
BASE COUNT          321 a          111 c          164 g          309 t
ORIGIN      
        1 gtgaaattgt tagaggtcct agtcaaatat ctattgatgt tacatataaa tgtaatttta
       61 gatgtaaaca ttgttacaat gcgagtggtg gagacaattg ttttatggat aatgagttga
      121 ctgatgatga actgttagaa cttacaaaag atatatgtaa tttaaagcca tataatattt
      181 gtttctgtgg aggagaaact ttactgagaa aaaaaatagt tttatcttgt gccactttat
      241 taaaagaggc aggtattcct aatattgcta ttgttactaa tggatcatta gtaactcagg
      301 atgttgcaaa agaattattc aaagcaggta ttaatcgatt tcaagtaagt ttagatggaa
      361 aaaatgctag tagccatgaa gcacttagat tatatccaaa cagttttgaa aaggcaatta
      421 atgctatcaa attatttaga gaattgtccg aagaagtgga tgttgctttt tgtcctacta
      481 gttttaatta caatcaatta cgagaagtat atcaactagt aaatactctt aaagtgaagt
      541 ctttaagaat tcaaccattt atgcttagtg gtaggggaat gcaaaatagg gaaatagtgc
      601 caactgtttc tcaatataga aaactagttt ctgatattaa ctatattaaa agaaatgaag
      661 attataccaa gatagattgg ggagatccaa ttgatcatat catccgacac agaactttat
      721 tgaaagattt ttcgaatttt gtttctatta ggtcagacgg aggattaatg gcatcacctt
      781 acttgccaat tcaagttgga aatattaaat gtcacagtct ttctaaatat tggcgagcag
      841 gactttataa agttttttct ttagatttag ttcaagatat agctactcaa gtttcttcaa
      901 tacaa
//
LOCUS       DACDKL010000381          904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.392, whole genome shotgun sequence.
ACCESSION   DACDKL010000381 DACDKL010000000
VERSION     DACDKL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.392"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            32..>904
                     /locus_tag="IV329_002902"
     CDS             32..>904
                     /locus_tag="IV329_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8941991.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          313 a          157 c          170 g          264 t
ORIGIN      
        1 ccgaccaaag aattataagg agtgagtaca aatgacctat acccatctta caacggatga
       61 acttgtaata atagagtctt atttcaaaat gaatcaatct gttgctaaaa ctgcccattg
      121 cttgaatcgt tcaagacaaa cgatccataa agtatatctg ttcttcaagc aaggaaaatc
      181 agccttagaa tattatcaac agtataagaa aaacaaatca aactgtggta gacgtccgct
      241 tgttttaccc gaggaacaat cagaatatat tcaaagaaag gttgttcaag gatggacacc
      301 cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt tctgctcgta ccatttatcg
      361 tatgttcaaa aaggggctat ttgattcttc tgacttaccg atgaaaggca agcgtaaacc
      421 gaatggacat caagaaagac gtggaaaaca aactttccgc cgctctattc atgaacgtga
      481 aaaggattat agccaattct caaatgagtt tggtcacctt gaaggtgaca ctatcgtagg
      541 tctgaaacat aaaagtgctg taattacctt agttgaacga ttatcaaaag ttatcatcac
      601 attgaaaccg tgtggtagac aagcgattga tattgaaaaa aaattaaatc aatggtttga
      661 atctgtaccg aaaaacctat tcaaatccat cacttttgat tgtggaaagg aattttcaaa
      721 ttggaaacag atcagtaatg tcaatgatat tgccatttat ttcgctgatc caggaacgcc
      781 gtctcaaaga ggcctaaacg agaattctaa cggattgtta cgtagagatg gtttattgaa
      841 atctatggat ttcaattcag tagatgaatt ttttattcaa tctgtcgcat ctaaacgaaa
      901 taat
//
LOCUS       DACDKL010000382          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.393, whole genome shotgun sequence.
ACCESSION   DACDKL010000382 DACDKL010000000
VERSION     DACDKL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.393"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV329_002903"
     CDS             <1..888
                     /locus_tag="IV329_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8941992.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDKL010000383          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.394, whole genome shotgun sequence.
ACCESSION   DACDKL010000383 DACDKL010000000
VERSION     DACDKL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.394"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(483..776)
                     /locus_tag="IV329_002904"
     CDS             complement(483..776)
                     /locus_tag="IV329_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8941993.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          263 a          153 c          125 g          330 t
ORIGIN      
        1 cgaataaacg gtgttcaatt agctgatctt gtattcactt aaaaaatggt aaagtacgag
       61 ttgctattcc ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc
      121 attggaataa aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc
      181 atctgtccaa aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga
      241 gaattatctt tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga
      301 ttttactgac gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc
      361 atattttgaa taacgttctt ggctttgtat tatagataca gatgacaaaa aaagttcaag
      421 tatgcggtgt agaaccaata tcgcatactt gaactttttt caatgagtat aaattaaaac
      481 ttctaatttt caattcccat catctgatat gcccgaccgt ctgaacttct tttgaaaaag
      541 ccaaatgtga ttagataacg tctttgttcg acataatcgc cactagaaaa tacgttcagc
      601 agtatcgaat taacctctgt ttctgtataa gtcactccat ggtcaaagcg tttcaagact
      661 tctttcaata ggatgtattt cttttttgtt gaacttggta cttgttttac ttttccatca
      721 ttcatgaatg tgtctaaaac tttcatcttt tcttgctccg tataagtata aaccatcttc
      781 tttctccttt tgattctaag aatactccat ttatgttaga atattctgga ttttttatta
      841 aagcggttac tcttaagaaa gatattatca t
//
LOCUS       DACDKL010000384          856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.395, whole genome shotgun sequence.
ACCESSION   DACDKL010000384 DACDKL010000000
VERSION     DACDKL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.395"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..>856
                     /locus_tag="IV329_002905"
     CDS             37..>856
                     /locus_tag="IV329_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8941994.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          296 a          130 c          155 g          275 t
ORIGIN      
        1 ttaccacaac acattcacag aaaagaggac ttccatatga acgattttac tacagaaatt
       61 atggaaatac taatcaataa aggtgatttg gatgatttgt ttcgtcgtca cctagaattg
      121 gctattaacg ctttattaca agctgaatta acggtatttc ttgactatga aaagtatgat
      181 agagctggat ttaattcagg taattcccgt aatgggaatt attcgcgctc ttttaaaaca
      241 gagtacggtg aattgaattt agtaatacct agagatagga atggagactt ttctcaacaa
      301 actttacctg cttataaaag aaccaatgac tctttagaaa ccactattat tcagctagtt
      361 caaaaaggaa ttactatgtc cgaaatctct gatctaatcg aaaaaatgta tggtcattat
      421 tacaaaccac aaactatttc aaatatgagt aaaattgtgt ctgaagatgt ttcggcattt
      481 aaagaaagaa ctttagaagc taaatactca gtcattttta tggatgctac tcatattccc
      541 ttaaaaagac aaaccgtatc aaaagaagct gtctatatag taattggtat caggttggat
      601 ggaactaaag aggttctagg atttactata gctccaactg aatctgctta tgtttggaaa
      661 gagattcttc aagatctaaa agaccgtggt ttagaagagg ttttgttagt cgtaacagat
      721 ggtttaagtg gtattgacga tagtattcat agtgtttatc caaatgctca atttcaacaa
      781 tgttgtgtcc atatatctag aaatatcgct cataaggttc gtgttagtga tcgacaagaa
      841 atctgtagcg atttca
//
LOCUS       DACDKL010000385          849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.396, whole genome shotgun sequence.
ACCESSION   DACDKL010000385 DACDKL010000000
VERSION     DACDKL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.396"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            87..356
                     /locus_tag="IV329_002906"
     CDS             87..356
                     /locus_tag="IV329_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8941995.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="IV329_002907"
     CDS             349..606
                     /locus_tag="IV329_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8941996.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          325 a           94 c          160 g          270 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtg
//
LOCUS       DACDKL010000386          837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.397, whole genome shotgun sequence.
ACCESSION   DACDKL010000386 DACDKL010000000
VERSION     DACDKL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.397"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            270..785
                     /locus_tag="IV329_002908"
     CDS             270..785
                     /locus_tag="IV329_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAP8941997.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
BASE COUNT          277 a          103 c          182 g          275 t
ORIGIN      
        1 tttgttttat aacaccctag ggggcttaat tggtgctttg ctatattggc tattctaccg
       61 attaaataag tatatagact taaaatagaa ctgctttgaa ttttaatatg gttaatattt
      121 ttctgcagac aggattgtgt aatctcatca agaatacttt acatgccatc tatttttgct
      181 aaaacattgt tgttatatct tcaagaggta gggaatttta ttgctttaaa tgaggtcgag
      241 cgaattaaaa aattggtaaa atatataaca tggtggtagt tatggtaaaa gtgtttgata
      301 taattaagtt ttcggatttc agaggatata ctgctgttgc attggataat gagactcttt
      361 tctcgattgg tattgagttt cacattaagc aaataaatca aggatttagt cataataaat
      421 atactatgcg agggctacat tatcagagtg gagagcatgc tcaaggaaag atagtgatgt
      481 gcctacatgg tgaaattatg aatatcgcac ttgatattcg acaaggatct tccacttatg
      541 gatgtgtgta tgtcaacaca ttgagtgaaa gcaatggtaa atgtgcgtat atcccacgtg
      601 gatatgctca tggctatctt actatggagg acaatacgct agttcagtgg tgcgtagata
      661 atgatttcaa ttctaatgct gccaaatgta taagttatga aagcgtagat attgaatggc
      721 cggtcagtcg gcagtgtttc gttataagtg agaaagacaa aaatgtggaa agattaagct
      781 tttgattaaa atgagctgtg gagaaaagaa ggatactgga aaataagaag aacaata
//
LOCUS       DACDKL010000387          818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.398, whole genome shotgun sequence.
ACCESSION   DACDKL010000387 DACDKL010000000
VERSION     DACDKL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.398"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>818
                     /locus_tag="IV329_002909"
     rRNA            <1..>818
                     /locus_tag="IV329_002909"
                     /product="23S ribosomal RNA"
BASE COUNT          249 a          162 c          239 g          168 t
ORIGIN      
        1 ccgatatgct ttggggagct gtacgtaagc tatgatccag agatttccga atgggggaac
       61 ccaacatctt taataggatg ttacgattgt gtgaatacat agcacattcg aggtagacgc
      121 agagaactga aacatctaag tacctgcagg aagagaaaga aaattcgatt ccctgagtag
      181 cggcgagcga aacgggaaaa gcccaaacca gcaagcttgc ttgttggggt tgtaggactc
      241 caatatggta gttctttcag atagtcgaat gacttggaaa agtcagtcaa agagggtaaa
      301 aaccccgtag acgaaatgtg gaagacacct aggaggatcc tgagtacggc ggaacacgag
      361 aaattccgtc ggaatccggg aggaccatct cccaaggcta aatactccct agtgaccgat
      421 agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg gaaggggagt gaaatagaac
      481 ctgaaaccgt gtgcctacaa caagtcaaag cccgttaatg ggtgatggcg tgccttttgt
      541 agaatgaacc ggcgagttac gattgcatgc gaggttaagt tgaagagacg gagccgcagc
      601 gaaagcgagt ctgaataggg cgtttgagta tgtagtcgta gacccgaaac catgtgatct
      661 acccatgtcc aggttgaagg tgcggtaaaa cgcactggag gaccgaaccc acgtacgttg
      721 aaaagtgcgg ggatgaggtg tgggtagcgg agaaattcca aacgaacttg gagatagctg
      781 gttctctccg aaatagcttt agggctagcc tcggaatt
//
LOCUS       DACDKL010000388          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.399, whole genome shotgun sequence.
ACCESSION   DACDKL010000388 DACDKL010000000
VERSION     DACDKL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.399"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IV329_002910"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IV329_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8941998.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDKL010000389          794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.400, whole genome shotgun sequence.
ACCESSION   DACDKL010000389 DACDKL010000000
VERSION     DACDKL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.400"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..500
                     /locus_tag="IV329_002911"
     CDS             <1..500
                     /locus_tag="IV329_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8941999.1"
                     /translation="MVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTI
                     VLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQW
                     IPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVL
                     KKYKK"
     gene            610..>794
                     /locus_tag="IV329_002912"
     CDS             610..>794
                     /locus_tag="IV329_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942000.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQT"
BASE COUNT          238 a          108 c          164 g          284 t
ORIGIN      
        1 taatggttag tagtattggt ggggcgtttg gtcacagcat atcccccggg atttttggat
       61 tattatctgg aatcatattt gtttatatta gctataacta ccctagaaaa ggaattttta
      121 ctatttacac aattgtttta ttgttatttt ttacgttaat gggcggggcg tacttacctt
      181 ggtttatcag ttcgattagt atggcgatct tagctgatat tattctatct gtatttggat
      241 atgatcgagc aattcctcaa gttgcgtctt gggctctaat gcaattaggc agtgctgccg
      301 gacagtggat tcctatatgg ttctttacag atcgttttcg tcaagattgg atagataaag
      361 gacaatctgc tgcaacaatg gatgcaatga tccactatgc ggtaggtata tggggaatca
      421 tttcagtgtt agttgtagca agtttatcta tgattggtgt attgattgga agaaaagtct
      481 taaaaaaata caaaaaatag aacgtagttt accttttatc tgtttgtaca tctctaattt
      541 acgctattac ggaaagcatt aacagttgta aacgaataag agaagaaatt gaatgcgggg
      601 gaggaaaaaa tgaaagtata caaaaaatta tttagttatg tccctaaaat gaaatactac
      661 ggatggttag ccactttatt ttctgatttt tctgtcgtat taacagtcta tggatattac
      721 agtatttaca aatttcttca gtctctaatt attgtaggag atgaatattt agctaagagt
      781 tatgcggttc aaac
//
LOCUS       DACDKL010000390          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.401, whole genome shotgun sequence.
ACCESSION   DACDKL010000390 DACDKL010000000
VERSION     DACDKL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.401"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..670)
                     /locus_tag="IV329_002913"
     CDS             complement(62..670)
                     /locus_tag="IV329_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8942001.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          215 a          141 c          148 g          288 t
ORIGIN      
        1 caaaaaaacc gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg
       61 cttagtacca gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag
      121 atgttacata ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt
      181 atgaagcatc taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc
      241 ctgattcttt ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag
      301 ctggggcagc aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg
      361 ttgctgtttc agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag
      421 aatcatttga agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga
      481 tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga
      541 tcttagataa tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag
      601 ttcctacaaa cattgcagta gcaccagcag caaaagttgt acctaaaaca attttcttaa
      661 atgaattcat attaaactcc tttttttctc cgttttttat tctttcgacg ctattaactt
      721 tatcatttag aaatatttca taggtaaaag aaacatgaca tttaatgaca aaataacgaa
      781 ttatttaata tt
//
LOCUS       DACDKL010000391          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.402, whole genome shotgun sequence.
ACCESSION   DACDKL010000391 DACDKL010000000
VERSION     DACDKL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.402"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..550)
                     /locus_tag="IV329_002914"
     CDS             complement(152..550)
                     /locus_tag="IV329_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8942002.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(547..783)
                     /locus_tag="IV329_002915"
     CDS             complement(547..783)
                     /locus_tag="IV329_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8942003.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          248 a          133 c          118 g          293 t
ORIGIN      
        1 ttttgtattc cttttaaatc aattatttca aaattaatcc atattttcca tatttttatg
       61 aaaaaactat tttgtatgat taaacataaa aaaatcagca tattccttaa tgacatgctg
      121 atctaattga gaaataaagt ttttaattcg ttcattttat tgcggtttgt cgtaaataac
      181 tggttatctt ggactttagt gaatcgaaat ccaatgaact agtggtaatc attatgataa
      241 aatcaacagc ttcttgacga gaaaaagcaa ctggacaatt attcaattga agaaacaaca
      301 acatggcaac aagagcagtt cgtttattgc cattatgaaa aggatgtttt ttagctaaat
      361 taattgctaa aatcgccgct ttttcataga tttcaggata gagttcttga ttgaatactg
      421 cttgtgaagg ctgtttgacg gccatatcta atgcatttgc atcttttaca ccgatcattt
      481 cgcctggcga gtgacgtaaa ataactttcg cgttgatttt gataatttct ttagtcgaaa
      541 gatacctcat aaatcaacca aatcttttaa cgcgtcttca tattgattca tgatcgaatc
      601 aactaatgaa tcaatatcag gttcttcttg tgctttgatc attcgtacag aaccatcaga
      661 ttgaaaaaca taactgattg cgtcacctgt ctgcacaccc atgaattctt ttacttctgg
      721 tgggatagtt gttacatcgc tattgccaga ttttctaatt ttatactttt tcattttttt
      781 catatttcgc ca
//
LOCUS       DACDKL010000392          788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.403, whole genome shotgun sequence.
ACCESSION   DACDKL010000392 DACDKL010000000
VERSION     DACDKL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.403"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            147..>788
                     /locus_tag="IV329_002916"
     CDS             147..>788
                     /locus_tag="IV329_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8942004.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTK"
BASE COUNT          288 a          114 c          127 g          259 t
ORIGIN      
        1 agacaagatt gtctagataa tgtgtgagat aaaatttttg gcaggttatc atacataaga
       61 caaaatgttt ttaaaactga taacgtattt attccacaac aattacgcat ttatttctga
      121 gaaaaatata aagcaggagg attacaatgc tgaaaactta ttccttaaca aaagatacat
      181 tattagtttc gtcagaatca aatatcaaaa attcttctgt tgtttatgtt tatggtgacg
      241 aacgagaata tatcgaatct tctgaacata aatatgactt taaaataggc aatatactaa
      301 cgtttgatga tcgagttgca tatgacacga tgatagataa gaacgatgaa aaaagtttgc
      361 tcgtaagttt tttgtttccc gaggtacatg aaggtgggca tctagatacc ctgacgacat
      421 ctgttgtttt cttggtattt aaaaataagt tgataatttt tacaaaacaa aaattaaaat
      481 tcattcccga actcttatcc aatatggttt tacgtgatgt caatcatttc gtggaggaag
      541 tacttttaaa gatcatgcaa aaagatttct atatgatgct taacgacctt cgtggagtca
      601 aggaaaaaat tgatgattta gactcagaaa tatcaacgtc cggttcactc aaaccaacgt
      661 ttggtgattt attgactctt caaaaataca tgatagcttt gtcaacaact tataaagcta
      721 accataaagc gcttgatttc attaaaaaaa atttcacaac tattgacgat attgatttta
      781 gtactaaa
//
LOCUS       DACDKL010000393          779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.404, whole genome shotgun sequence.
ACCESSION   DACDKL010000393 DACDKL010000000
VERSION     DACDKL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.404"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..442
                     /locus_tag="IV329_002917"
     CDS             35..442
                     /locus_tag="IV329_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942005.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            456..>779
                     /locus_tag="IV329_002918"
     CDS             456..>779
                     /locus_tag="IV329_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942006.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYD"
BASE COUNT          293 a          102 c          137 g          247 t
ORIGIN      
        1 tttattaaat aaatgtaaaa attaggagaa atcaatgtct tatagagtac aattaataat
       61 ttcagaagat gtagaaagtc agcaatttgg cactaatgtt attaataaag taattaatcc
      121 agctctttct attaatgcgc ctttaattcc tactgcttta tcattttctg taacagctgt
      181 ggtgtctgag atagaggata cagaaaagat aaaaatagta gaaattgaag ttttaaacaa
      241 gaatgaaaaa caaatttttt ctacgggtga agtatcggta agcttgcctc ctcaagttaa
      301 cgatataaac tttaatataa acgccagaaa tgtcttggtt gaagaagcag gagagcatta
      361 tgctgttgtt aaatttaatg gaactgagat tggaaggcaa atctttgata tcaaggtcaa
      421 caaaccagtg gaaaaaaatt aaggagacga tacggatgct tgatatagta ccatcaaata
      481 cacatcggag tggtagctta ataaattttg ctgcgatttt tgcttgcgca tcggtaattc
      541 tagcatctcc agttgttgtg gatgcaagtt ctactccgaa aactaaaaat gataatcaac
      601 ttgtaattac tacaaaaaat gagattaaca caatttcaag taatgatggc aaaatttttg
      661 atgtgataaa tacggtttta aaaagaaaag ctgaaatcaa tataaaatat gatgaagatt
      721 taaacttgta tttctttgtt atcaaaacaa catctgaatt gttttcctca gattacgat
//
LOCUS       DACDKL010000394          772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.405, whole genome shotgun sequence.
ACCESSION   DACDKL010000394 DACDKL010000000
VERSION     DACDKL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.405"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..>772)
                     /locus_tag="IV329_002919"
     CDS             complement(109..>772)
                     /locus_tag="IV329_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8942007.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          213 a          178 c          149 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gc
//
LOCUS       DACDKL010000395          760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.406, whole genome shotgun sequence.
ACCESSION   DACDKL010000395 DACDKL010000000
VERSION     DACDKL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.406"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>760
                     /locus_tag="IV329_002920"
     CDS             <1..>760
                     /locus_tag="IV329_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8942008.1"
                     /translation="ETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVPSS
                     YNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQHK
                     GGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVNFD
                     FFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDEEA
                     QLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVS"
BASE COUNT          294 a           94 c          150 g          222 t
ORIGIN      
        1 cagagacgag gcaagtaatt gatttaagcg gtctttggag atttaagtta gatacaaatg
       61 atgacttaca agaagaactt gctttagaac caatggttga aaaagagagt tatccaattt
      121 cagttccttc ttcttataat gatttatttg aaacagagaa cattagaaat catgttggat
      181 gggtgtggta tgaaaaagaa gtgattcttc ctaaaaaatt attagaagaa agattagttc
      241 ttcgttttgg atctgcaaca catgaagcaa aagtattttt gaatggaaaa ttgattaatc
      301 aacacaaagg agggtttacg ccatttgaat cagaaattaa tcagtttata aaagatggaa
      361 gtaacagaat tacagtggct gtaaacaatg ttattgacga tagcacttta cctgtaggga
      421 ttttgaaaga aacgatacag gaagatggag acataaaaaa agaaaatttg gttaattttg
      481 actttttcaa ttatgcaggc attaatagac ccgttaaatt gtatacaact ccgaaagact
      541 atataaagga tgtcactatt aatacaaaaa taattgattc agatgcaaat gtagaattta
      601 atatcgatac agaatggacc ggagaaattc taatagcaat ctatgacgaa gaagctcaac
      661 ttatagcaga agctaagggg aaagaaggag tgattcaact taagaaccca acactttggg
      721 agcctctaaa tgcttactta tatagtatga aagttagttt
//
LOCUS       DACDKL010000396          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.407, whole genome shotgun sequence.
ACCESSION   DACDKL010000396 DACDKL010000000
VERSION     DACDKL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.407"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(431..631)
                     /locus_tag="IV329_002921"
     CDS             complement(431..631)
                     /locus_tag="IV329_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8942009.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          226 a          150 c          101 g          274 t
ORIGIN      
        1 ggatttaatg aactactttt tcctctttgt cgtttttata tattcgtatt ccttcaaaca
       61 gtaggatagc ctctttcaat gcttgatatt tgaactgtcc gttacttaaa aatttagatg
      121 tgccgccctc ttcctagaac atattctttt ttactttttc attaattgac agaccaatcg
      181 aacgaaactg taaatttcga taggataatg attggtcaaa ctcattcatt ttcaagacat
      241 ctcgattcat attcaagata ggaatctttt tatcaacatc actttattcg tattttcctc
      301 taccataaaa gcttcacgct ccacaaaatt gttttataca ctactattca gcccattcaa
      361 cgaccaatga attttttcgt acaaaaaaac cgtcttttct ttcgaaaaga cggttaaaag
      421 tcaattttta ttaagcttta acaatgtttg ttgcttgagg gccacgttga ccatcttcaa
      481 catcgtaaga cactgcttgg ccttcttcta aagttttgaa gccgtcacct tggatagctg
      541 aaaaatgtgc aaatacatca tttccatctt caccagtgat aaagccaaaa cctttgtctg
      601 cgttgaacca ttttactgta ccgttattca taaataaatt cctcctgata cgtatcaatg
      661 tacgtttttt cgttgcaata aatatttgat aggcaaaagg agttttatct atgtagatat
      721 atcgctcaaa ttacgtttca aaatagatta c
//
LOCUS       DACDKL010000397          745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.408, whole genome shotgun sequence.
ACCESSION   DACDKL010000397 DACDKL010000000
VERSION     DACDKL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.408"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..180
                     /locus_tag="IV329_002922"
     CDS             <1..180
                     /locus_tag="IV329_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942010.1"
                     /translation="KTLNDFTSNSISKGTGINLSTIKKLKSGERSVEKLNLLDAIKIT
                     EFAMKNGKAEIEIWR"
     gene            complement(397..653)
                     /locus_tag="IV329_002923"
                     /pseudo
     CDS             complement(397..653)
                     /locus_tag="IV329_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          252 a          111 c          133 g          249 t
ORIGIN      
        1 aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat caatttaagc
       61 acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct tttagatgct
      121 ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat atggagataa
      181 ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg tgcttgaagg
      241 gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac aacgatatct
      301 cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa gcaataatcg
      361 tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg agcttaggca
      421 ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg aaagtattct
      481 ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct ttcaaaggca
      541 gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt cactactaaa
      601 ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc catggtatat
      661 caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc actatgtctt
      721 aattgtcggt tttcgagtta ccgaa
//
LOCUS       DACDKL010000398          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.409, whole genome shotgun sequence.
ACCESSION   DACDKL010000398 DACDKL010000000
VERSION     DACDKL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.409"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            250..>741
                     /locus_tag="IV329_002924"
     CDS             250..>741
                     /locus_tag="IV329_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP8942011.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIF
                     DFNA"
BASE COUNT          265 a           84 c          145 g          247 t
ORIGIN      
        1 gatgaattct tggcggatat cagtctattt ataatgttta ttgatgtaat tgaagaaaca
       61 tcaagaaatt ttttgctgtc taagttcaga aaatttcgta tgaaaaatga taaacaaaaa
      121 ttattggcaa atgagatttg gagtagttta gaagtcgaag aaagtaaaca agaacttttg
      181 ttgaaatgga tactttttct tgtaaagaag ttctatgtga aaataccaga aacccattgt
      241 ctgatagtta tgcacggtaa aataacagct tctgcgatcg caagggaaac aaataagttg
      301 ctgaatacat atgtttatga ggctttcgat atgccaattg aaggtgagac tagtgactta
      361 attaacctaa tcaacaattt ttgtaaaagt atagatacga cgaatggact cattctgcta
      421 gttgatatgg gatcattgga acaaatgtat gaaaaaattg aatccaatgt aataggtgat
      481 ttggtaattt taaacaatgt ttctacagct ttagcgattg aatgtgggat tcaagtttgc
      541 caaaaaaagc caattagtca tttttaccag atggattttg attcgtttca agttaaagtt
      601 cagtattata aaggattatc acaaaagaaa aatgtaatag tatcttgtct ctctggtgag
      661 gggatttctg aaaaagttaa ggatatttta aaacggtatc tcgataatca agtggagatt
      721 ttgatttttg attttaacgc a
//
LOCUS       DACDKL010000399          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.410, whole genome shotgun sequence.
ACCESSION   DACDKL010000399 DACDKL010000000
VERSION     DACDKL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.410"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..141
                     /locus_tag="IV329_002925"
     tRNA            68..141
                     /locus_tag="IV329_002925"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:102..104,aa:Ile,seq:cat)
BASE COUNT          227 a          125 c          130 g          259 t
ORIGIN      
        1 gcgataacag gtttgttgcc acacattcta ctggttgatt gtttatggct ttatgtggca
       61 acaaccagta cccttagctc agttggtcag agcagacggc tcataaccgt ccggtcgtag
      121 gttcgagtcc tacagggtac attaacgtag ccatttgaat cgttctgtgt tagaattttt
      181 tgaagagtat tatacaagct aaagcttttc ttcattgcca ctcaaatgag tggctttttt
      241 atgtatcctt ttatggatta atgaaaggat gtttcacata gttatatttc tgtatatttg
      301 aaaagtttta ctttgatttt taaatagaaa gacatttggg ttatattgtg agataataat
      361 aaagaagagt ttaaagcgca ccccaaacca cttccccata agtgtgttac gctttaaact
      421 cttttatatt tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag
      481 gcttacaatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
      541 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatctcctt
      601 tcacaagtat ggtgataaaa ttcgttccgg gctacctttt taggtagcct actttaatct
      661 ttataccttt ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgcta
      721 cgtgcgtccg gcacgtcagt c
//
LOCUS       DACDKL010000400          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.411, whole genome shotgun sequence.
ACCESSION   DACDKL010000400 DACDKL010000000
VERSION     DACDKL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.411"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            112..480
                     /locus_tag="IV329_002926"
     CDS             112..480
                     /locus_tag="IV329_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8942012.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          267 a          120 c          112 g          242 t
ORIGIN      
        1 tatcatataa atatagcggt tacaacatta cttgtgtttt gttcgttttt tatacataac
       61 tattgttttt ctggtaaaat aaatcaaacc acttaatgga ggcgttttag aatggaaaaa
      121 tttcagccga tcatcaatca aatcaaagaa aaagaacatc aacaaaagat caaagacctt
      181 tttgctaaga taacagaaac gtatccccag ctagacacaa ctgtaaaatg gaaccaacct
      241 atgtttactg accatggtac ctttattatt gcatttagtt ttgctaaaaa tcatttttct
      301 atcgcaccgg aaaaagcagc gattcgtgcc cttgaaaaaa atatccaaga agcaggctac
      361 gtatatacgg ataacgtcat caaagttcct tggaaaagtg taatcaactg ggaattgatt
      421 gaacaattga tttcatttaa tattgaagat aaaaaaggtc atgataaatt ttggcgataa
      481 agctttatac gaataaacct tttactggcg aggaaactat tcatcacctt atacaggttt
      541 tgttcaatcc tatccttttt atcatccctg gttaaacact taattgacaa agtatctgct
      601 atttttataa ggagtctttc ttgtgctgaa aaaaatcact gattgaatgg ctttgaattt
      661 tctacatagt aaaattattt tttcagcaat tttcctgcaa aacttttgaa taaggaaaaa
      721 atacgagaaa atatcagtga t
//
LOCUS       DACDKL010000401          714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.412, whole genome shotgun sequence.
ACCESSION   DACDKL010000401 DACDKL010000000
VERSION     DACDKL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.412"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IV329_002927"
     CDS             <1..395
                     /locus_tag="IV329_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942013.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..>714
                     /locus_tag="IV329_002928"
     CDS             398..>714
                     /locus_tag="IV329_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8942014.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTP"
BASE COUNT          278 a          122 c          138 g          176 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctat
//
LOCUS       DACDKL010000402          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.413, whole genome shotgun sequence.
ACCESSION   DACDKL010000402 DACDKL010000000
VERSION     DACDKL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.413"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..436)
                     /locus_tag="IV329_002929"
     CDS             complement(<1..436)
                     /locus_tag="IV329_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8942015.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          214 a          120 c           95 g          273 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc atttttatta
      661 ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACDKL010000403          693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.414, whole genome shotgun sequence.
ACCESSION   DACDKL010000403 DACDKL010000000
VERSION     DACDKL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.414"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..116)
                     /locus_tag="IV329_002930"
     CDS             complement(<1..116)
                     /locus_tag="IV329_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8942016.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTL"
     gene            348..>693
                     /locus_tag="IV329_002931"
     CDS             348..>693
                     /locus_tag="IV329_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8942017.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQ"
BASE COUNT          251 a          110 c          113 g          219 t
ORIGIN      
        1 tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt aattatctct
       61 ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag agacatattt
      121 ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt attttttgct
      181 caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt accagttcat
      241 cgacatacac ataatacctc gataaaaaag gggtgtcaac agctttgtaa aatttatttg
      301 caacacccct tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc
      361 caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa
      421 agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg
      481 acaaaaaaac tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat
      541 cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag
      601 aatatatttt gggaattatt ctttatagag atagcattgt actagatggt ataacatttc
      661 cagataaaaa cctgttccag catcttcgtc aac
//
LOCUS       DACDKL010000404          681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.415, whole genome shotgun sequence.
ACCESSION   DACDKL010000404 DACDKL010000000
VERSION     DACDKL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.415"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..563
                     /locus_tag="IV329_002932"
     CDS             <1..563
                     /locus_tag="IV329_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8942018.1"
                     /translation="GDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENE
                     LAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANY
                     FSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFS
                     RVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          238 a           83 c          124 g          236 t
ORIGIN      
        1 gtggagatac gattcggtct gaaaaaaatt attctatcat tgtttttgaa aagttagcac
       61 aaacgtctat aacattagga atggatataa tagaagcgta tcaaagtaga gatgctttga
      121 tacaagaaaa cgaattagct gtatcgttac ctgaggtctt aaaagtgagg gattctggga
      181 ttgtttatta tactaaggaa atcggaaaaa ctaagataga gcatctttct cccttaattt
      241 cttctgtcgt tcaattcatc ggcttaaata tctataaaag aattactgta aaggaaatag
      301 ctaattattt ttctgtaagt gaaactaaat taagagaaag ctttaagaat gaaatgcata
      361 ttacgattta taattatatc tctaagcgaa aaatttctac ggctaaaata atgttgaaat
      421 caaaccatac tatcagtgaa gtttctttag gtttaggatt ttcagattct tcacactttt
      481 cgagagtatt caaaaagtac gcaggtgtat ctcctaagca atatcaatta ggtttagtgg
      541 acaatttcaa caacattagc tgagaggttt tattttatta tgagactata tcatttacgt
      601 agtttggagg agttgaggaa attggatttt aacaaaaaat ttttttggga aatttctgat
      661 ttgagttgtt tcgtagaatc t
//
LOCUS       DACDKL010000405          677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.416, whole genome shotgun sequence.
ACCESSION   DACDKL010000405 DACDKL010000000
VERSION     DACDKL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.416"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            57..>677
                     /locus_tag="IV329_002933"
     CDS             57..>677
                     /locus_tag="IV329_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8942019.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLD"
BASE COUNT          263 a          104 c          117 g          193 t
ORIGIN      
        1 acgagcatag aactaaaata tcaaattcaa aaatgaaatt ttgagaggga ttaaatatga
       61 caattaaaac tagtctccaa aatcaagctt atcaatctat tcgtcagcaa attatctacg
      121 ctgacttaga acctggaaaa aaagtatcag aaaaaaaatt ggaagaaacg ctttctatcg
      181 gtcgtactcc aatacgtgaa gcattgattc aactccgtca tcaaggactt gttgatacaa
      241 ttcctcaaag tggaaattat atctcattaa tcgacttaga tttagcaaaa aatgctcgtt
      301 ttgtcagaga acatttagaa cgacaaatta tgctagagtg ttgtgccaag atgaataatc
      361 aaaagaaaca agttttagaa actataattg cagaacaaga aaaagctgtt agtcaaaaga
      421 atgctcgtag tttttttcat aatgataatt tatttcataa agcttgtttt gaaattgcag
      481 aacgggaaga agtatggcaa tggttggaag ataataatac gcacttggaa cgatttcgtt
      541 ggttacgagt aaaaacggaa ggattaaaat gggaaaaaat tatggaacaa catcatcaac
      601 tatttaaagc cttgaccatg caagaccctg aagaagctaa cttcttaaca tccttgcatc
      661 ttcatttaat gcttgat
//
LOCUS       DACDKL010000406          653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.417, whole genome shotgun sequence.
ACCESSION   DACDKL010000406 DACDKL010000000
VERSION     DACDKL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.417"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>653)
                     /locus_tag="IV329_002934"
     CDS             complement(<1..>653)
                     /locus_tag="IV329_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942020.1"
                     /translation="LFYIFSGLFSHILGFRLETNLRKKGISGLTEASFRFFDLNSSGY
                     IRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGLVLIAMLVIGVFL
                     LKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFKALNKAINDYAKF
                     AYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLFFLSGII"
BASE COUNT          254 a          112 c           92 g          195 t
ORIGIN      
        1 aaataattcc acttaaaaaa aacaacatca acaagtcaac agctaatatc ttgggagtgg
       61 taagagaagt tataaaaaag gttataggaa ctatcaaaat gcaaactatg ccaaaaaata
      121 gccattgaaa aagaacaaaa ggtgttttcc cactcaaaga atacttatat gcaaacttag
      181 cataatcgtt aattgcttta ttcaaggctt taaatgatgt tactttagca ccaaatatct
      241 ttataacttg aattccgcgg atatattcta ccgtttcaga gcttaacaca tctaacgatt
      301 cttgatattt ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa
      361 ttactaacat ggcgattaga accaatccaa ttttaaagtt aatgaaaaag cctaaggcaa
      421 ctgataaaac aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca
      481 tatgtgtttt agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat
      541 caaaaaaacg aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct
      601 ctaagcgaaa cccaaggata tgagaaaata agccagaaaa aatataaaat aaa
//
LOCUS       DACDKL010000407          633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.418, whole genome shotgun sequence.
ACCESSION   DACDKL010000407 DACDKL010000000
VERSION     DACDKL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.418"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            209..607
                     /gene="spxA"
                     /locus_tag="IV329_002935"
     CDS             209..607
                     /gene="spxA"
                     /locus_tag="IV329_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8942021.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          226 a           95 c          120 g          192 t
ORIGIN      
        1 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
       61 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
      121 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
      181 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
      241 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
      301 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
      361 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
      421 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
      481 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
      541 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
      601 attatagaag gataatacga aacttggaaa aga
//
LOCUS       DACDKL010000408          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.419, whole genome shotgun sequence.
ACCESSION   DACDKL010000408 DACDKL010000000
VERSION     DACDKL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.419"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>625
                     /locus_tag="IV329_002936"
     CDS             <1..>625
                     /locus_tag="IV329_002936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942022.1"
                     /translation="VFLPQITEYLNYRIFTTKVDDSVTSLSRLEFIKICASVFGPILT
                     VMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKELLTQIDREFYFLLNMFIEIQKD
                     QNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEIGSTIEQSGPLKGTVEYIKFET
                     FDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQIYLGRYFKMFH"
BASE COUNT          246 a           78 c          117 g          184 t
ORIGIN      
        1 gtatttttac cacaaattac tgagtattta aattatcgga tatttaccac aaaagtagat
       61 gatagcgtta ctagtttaag taggctagaa tttataaaaa tatgtgcatc tgtgtttggt
      121 cctattttga ctgtaatggt atttgtgaat actttaaata tgcagaaaaa aacagagcaa
      181 aagcaaaatg agcttgaagt aaagcaagct aaggctgaga acgagagagc aaaaaaagag
      241 ctgttaacac agatagatcg tgagttctat tttttattaa atatgtttat tgaaatacaa
      301 aaagatcaaa atgccaaaca agctgttatt gctttacaga agttggcaat ttttaacgaa
      361 aagggtttta atcaagacta tgcatttact tctaaagatt tatcagatag tgaaattggg
      421 agtacaattg aacaatcagg accgctgaag ggcacagttg aatatataaa atttgaaaca
      481 tttgatgtaa gcaagtcgat ggggaattat caaacaaaaa caaaagacgt tgctacaagt
      541 ttacacgatc agaaagtagc tatattgaat gaacaatatg aaaagatgca aatatatctt
      601 ggacggtact tcaaaatgtt tcacc
//
LOCUS       DACDKL010000409          623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.420, whole genome shotgun sequence.
ACCESSION   DACDKL010000409 DACDKL010000000
VERSION     DACDKL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.420"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IV329_002937"
     CDS             <1..379
                     /locus_tag="IV329_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8942023.1"
                     /translation="SIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLTNSNEGKYS
                     QTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDTEIGKILNM
                     STSGIWYQRKKAVEQLKMGYDLW"
     gene            373..618
                     /locus_tag="IV329_002938"
     CDS             373..618
                     /locus_tag="IV329_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8942024.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
BASE COUNT          242 a           93 c          103 g          185 t
ORIGIN      
        1 atctatacaa catcaatttg atgcctactg taaaaaagta ttacgaaacg aaactaagtc
       61 tattcgaaaa cgaaacatta acataacaga taatgaggaa ccattgacga attcaaatga
      121 aggtaagtac tcccaaactc attttgatga acaagatgtc tatttcttat ttggaatgga
      181 aatattaatt tcagatcaaa agttatcggc agccattgat catttatcgg aaataagaag
      241 aaaggttatc ctactttatt attttgcagg ttttaatgat actgaaatcg gtaaaatcct
      301 caatatgagt acaagtggta tctggtatca acgaaagaag gcagttgaac aattaaaaat
      361 ggggtatgac ttatggtaga caaccccaaa aaacggcagt atccattagt tcctctatcc
      421 attatccaat ctgctctaga agaagatcca atttcaatca agatgatact caaacattat
      481 catagttata tagctaagtt atgtttgaca aatggattta atgaggcggg gcaatttatt
      541 acctatgtag atgagtatat gctaagacag ctagaaatta aattgataga agcgatctta
      601 aaatttaaaa ttaactaact aaa
//
LOCUS       DACDKL010000410          618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.421, whole genome shotgun sequence.
ACCESSION   DACDKL010000410 DACDKL010000000
VERSION     DACDKL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.421"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IV329_002939"
     CDS             132..344
                     /locus_tag="IV329_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942025.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>618
                     /locus_tag="IV329_002940"
     CDS             506..>618
                     /locus_tag="IV329_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942026.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEG"
BASE COUNT          222 a           92 c          108 g          196 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggtta
//
LOCUS       DACDKL010000411          611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.422, whole genome shotgun sequence.
ACCESSION   DACDKL010000411 DACDKL010000000
VERSION     DACDKL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.422"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..>611
                     /locus_tag="IV329_002941"
     CDS             39..>611
                     /locus_tag="IV329_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8942027.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHH"
BASE COUNT          231 a           70 c          122 g          188 t
ORIGIN      
        1 tgatcgtgat aatcattatc atttagaagg agaaagttat gttagaagtg aaaaagctta
       61 atgtttgtta taacgatttt ttagcattga acgagatttc tttaaaaata caagaaggtg
      121 ctttaacagg tattattggt ccaaatggtg ctggaaaatc cacattgctt aaagcaatgt
      181 taaatattat ccctcatcag ggagaggtct ccattaataa tgaagatatc aataaaaaac
      241 tggtgaagat tgcatatgta gagcaaaaag ctgatattga ttttactttt ccaataaaag
      301 taaaagaatg tgtatctatg ggaacatatg ccggaatgaa agtttttcaa agaattaaaa
      361 atgcagaatg gcaaagagtt tcaaaggctt tggaaaaggt cgatatggga aaatattcta
      421 atcatcaaat aggagaatta tctggaggac aatttcaacg tgtactttta gcaagatgtc
      481 ttgctcaaaa tgcggatttc atatttttag atgaaccatt tgtagggata gacttagtta
      541 gtgaacgaat aattatggat acgttaaaaa aattgaaaca tcagggaaaa acagtgctaa
      601 ttgtgcatca t
//
LOCUS       DACDKL010000412          599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.423, whole genome shotgun sequence.
ACCESSION   DACDKL010000412 DACDKL010000000
VERSION     DACDKL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.423"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..>599)
                     /locus_tag="IV329_002942"
     CDS             complement(18..>599)
                     /locus_tag="IV329_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8942028.1"
                     /translation="KDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEI
                     SAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMH
                     NGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEM
                     VEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          217 a          109 c           73 g          200 t
ORIGIN      
        1 tgaaagctgt cttcatatta attctccttt tttaaataca agtaagaaat gcttcccaaa
       61 taataccaga aaacaattga aatgacatcg cgcttattga aaagtacatg agcctccacc
      121 atttcatcta taaaaataga tagtataaaa attaaaacaa taccaagaaa cgcataattc
      181 actttacttc taaaaaatgt tttgagtaaa ctaaagatat ttaacacata gaaagcatag
      241 atacatataa aagcaattat accacttgat acaagtactt gaagaaatcc attatgcatg
      301 tttccatgtg ctctttttag ttcattcaca tctgtagtat caagatctac ttttttcaaa
      361 aattttgcat cgctattccc atagaaatcg gtctctccat aaccaaataa caaagttggt
      421 cttataactt taagcgcagc tttccaaatc gtaaaacgac cagctgaaat ttcggagtta
      481 tcactagaat gctgtaatat tacttctttt tctggaagtg gagttttatc tgattcattt
      541 gactctataa cttctacttt tctatcaatc gatacaggaa ttgttgctaa aaaatcctt
//
LOCUS       DACDKL010000413          598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.424, whole genome shotgun sequence.
ACCESSION   DACDKL010000413 DACDKL010000000
VERSION     DACDKL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.424"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>598
                     /locus_tag="IV329_002943"
     CDS             <1..>598
                     /locus_tag="IV329_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8942029.1"
                     /translation="DQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEK
                     VSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKI
                     IENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQL
                     PFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINM"
BASE COUNT          238 a           68 c          109 g          183 t
ORIGIN      
        1 tgatcaagtt ctatttgtta aaaatagaac tgtaaaaact acaactattc agttgttaaa
       61 aactgaaaac aataacgcat taaggaaatt tgacattttt aatagttcga ttgaagttcc
      121 ctatatcgaa aaggtaagtg cagatgtatt aaaagttgaa gaaattactt atgaaaatat
      181 tctaaaagac ataaattttg atgtcaaagt tggagatgtt attggtttgg tagggaaaaa
      241 cggtgtgggg aaaacaacct tactaagaat actcatgaag attattcgcc ctaccaaagg
      301 aaagataatc gaaaataaaa aaaatttgtc atcgccgttt ttagtaatgc aagaaatgga
      361 ttaccaattt ttcacagaat cagtgaggag cgagttagcc ctggggaatg aaatagttag
      421 taaagatcag caagaaaaaa ttttaaaaaa aatggaactt tataaaatga aagatcaatt
      481 accgtttgat ttatccggtg gagaaaaaca aagattattg gtttctatag cttcattgtc
      541 aaaaactaat ttatttctat ttgatgaacc tacaagtggg ttggattata ttaatatg
//
LOCUS       DACDKL010000414          570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.425, whole genome shotgun sequence.
ACCESSION   DACDKL010000414 DACDKL010000000
VERSION     DACDKL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.425"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..211)
                     /locus_tag="IV329_002944"
     CDS             complement(<1..211)
                     /locus_tag="IV329_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8942030.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIK"
     gene            complement(341..>570)
                     /locus_tag="IV329_002945"
     CDS             complement(341..>570)
                     /locus_tag="IV329_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8942031.1"
                     /translation="SFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYD
                     ILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          217 a           96 c           73 g          184 t
ORIGIN      
        1 tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat
       61 aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc
      121 ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag
      181 taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac
      241 tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa
      301 agcataactg agaacaattc tcaatgcaag ctttttttaa ttaactcagt aattcaatcc
      361 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
      421 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
      481 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
      541 aacagactgt ctttttcgtc acaaatgatg
//
LOCUS       DACDKL010000415          561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.426, whole genome shotgun sequence.
ACCESSION   DACDKL010000415 DACDKL010000000
VERSION     DACDKL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.426"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..439)
                     /locus_tag="IV329_002946"
     CDS             complement(<1..439)
                     /locus_tag="IV329_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942032.1"
                     /translation="MTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRLLK
                     GDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSNLF
                     FGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYR"
BASE COUNT          161 a          127 c           62 g          211 t
ORIGIN      
        1 aacgataaac tcccttaaca ccacttgtat tatttttcgg taattttgac gttagacact
       61 ctattactgt cccttcaaca catgtccttg tttcttgcct ttttaaacat ccacaacttt
      121 gagtatgtcc aaaaaataag ttactataac ttatctcaca catactgcca cactcacaat
      181 gacaaaccca ttttgttttc catgaacagt tattgtccaa aggatacttt gcagtaagta
      241 atccgaattt tttattttca atgtgatgct tttttgattt caaacatcca cagcttttta
      301 catcaccttt tagaagtcgc tttgccgata ctaacttttt tctcccacac agacattgac
      361 attcccaaac aacagttcct tcacttcgca gtttagttgg cttgattgca actaacttac
      421 caaaagtttt atttgtcaaa tttttatatt tatgcctatt tttataattt ccacatccac
      481 aacttttggt tctacctgat agtaagtttg atcgatatac aatcgtttca ttcccacagt
      541 cacataggca acgatatttt t
//
LOCUS       DACDKL010000416          555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.427, whole genome shotgun sequence.
ACCESSION   DACDKL010000416 DACDKL010000000
VERSION     DACDKL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.427"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          187 a           80 c           67 g          221 t
ORIGIN      
        1 cctcaaataa tccttactaa tactgaaacg taaaaaaaat aaacggattt caaacaaaat
       61 acgtttattt tttctattga ttttagctca aaatcgattc aattcgagtg aaatttgctt
      121 caattttcaa ttggtttttg ttcaaatttt gaaacatttt tgctcaaaaa atgagtgaat
      181 ttgtttcaaa agaaaaacaa atacgatcga aaaaaatttg aactcataac aattttttgt
      241 ttttttcaac gtattctctt tcaattttca ctaaaaatta ggaagaaatt gattcaatgt
      301 cttttcaaaa aagaatgttt ttctctcaat ttttttgacg tttctatcat gaatcacgag
      361 aaaacttggg acacttttaa catggtaacg ttgtaaaaaa gtttgaagtt cttcttgtct
      421 tggttctttt cttgtttcaa ttcgatatac attcctagag aattttttgt cgtatctttc
      481 caagaaagat tcaacttctt ggcatttttt acaatcattt cttgctagat acacaaaaaa
      541 tgattgattc gtttt
//
LOCUS       DACDKL010000417          555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.428, whole genome shotgun sequence.
ACCESSION   DACDKL010000417 DACDKL010000000
VERSION     DACDKL010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.428"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          199 a           89 c           92 g          175 t
ORIGIN      
        1 ggagcgttct caaatgagaa catgctccta aaactacttt attggcgtat ggaaaaatta
       61 tacttgaagt ggatagcggg ttgaataagt tgataagcat caaaacattt ataaaaatta
      121 ttcaagacta tgtctgttcc atatacaaat atgaaaatca gtaaactcca tttaactttt
      181 ttcgtagtgt gtctttttat acatgtttat cgttattaat agtgaatagg aaaaaaacta
      241 tttttgatca ttgacaatta aatacaattt ttgaataacg atccctgcta ttcgagctag
      301 aaactcaagt acgccatgac ctgattagcg acaaatactt agagatttct ttttgttctg
      361 ggaaaacaaa atgccatgac aatagcagca aataatgata cttccgtaag gctcgccgta
      421 aactggggag aggtgaaatt cctatgtgct tacccaaagc aattcaaaaa tttcataact
      481 actattttag gagatgattt tatagaatcc agagtctacc tgaataaaaa acaaacctgt
      541 gcatttctag gaatc
//
LOCUS       DACDKL010000418          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.429, whole genome shotgun sequence.
ACCESSION   DACDKL010000418 DACDKL010000000
VERSION     DACDKL010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.429"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            360..>546
                     /locus_tag="IV329_002947"
     CDS             360..>546
                     /locus_tag="IV329_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942033.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTR"
BASE COUNT          216 a           64 c          101 g          165 t
ORIGIN      
        1 ggagtaaacg aaatgatgaa agtcagattg agtttttaaa tgataccttc ctcagaactt
       61 caaaacgaat tcaaagattc gttgacaaat cttgagcaag atcatttaaa aaaatatttt
      121 ctcttcattt gacgaaaaat gtttagaaat cttatataag tgaagataaa gtaattggtg
      181 taattaattg tagagaatag cctttgagca attgaagcga tacaattatt tagaaaatga
      241 acttcctcca gcatactaca tgattcctta cgggacgatg ttaaagaaga aagcaagaaa
      301 ttttagaagt attagtagat aaactaatgg gcttttctca atttaaggga gtagcccaaa
      361 tgtttatcaa atatggtgca gatacagtag caaccatgtt tacagaagaa tatcaagtag
      421 actattatga agtaatagat aaatgtgtac attttgttta tgaaaaagtg tggaatttta
      481 tgaattttgc gataagtgaa gaaatcttac gagtacaaga aaacagaaca ggaatattta
      541 ccagag
//
LOCUS       DACDKL010000419          545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.430, whole genome shotgun sequence.
ACCESSION   DACDKL010000419 DACDKL010000000
VERSION     DACDKL010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.430"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..291
                     /locus_tag="IV329_002948"
     CDS             <1..291
                     /locus_tag="IV329_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP8942034.1"
                     /translation="MLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAI
                     GDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT          203 a           71 c           95 g          176 t
ORIGIN      
        1 atgctaaatg tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt acctcaaaat
       61 gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa gaaacctttg
      121 agaatcgcga tcggagatag tttaaatgac ttatctatgt ttgaaagtgc agatattagt
      181 tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt cactgtttca
      241 aatgataaag acggagtatc tcaagcattt gaaagaattc ttaccatata aactaattaa
      301 ggtagtatag aacatagaat tctaaatacg gaaaaatgga gcataaattc cagtacaaat
      361 agtacttctt cagtgtcaaa cgaagcgaat ataacataga tatttaggat tgtgacaaaa
      421 atcatgtcac aatctttttt tcgaataaac ttattttttg aagtcggcca tttatcgtaa
      481 tttctttgca taatacgaac aaattttcgg ttattgatga aaaaaaacat tgaaaatggt
      541 atgat
//
LOCUS       DACDKL010000420          544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.431, whole genome shotgun sequence.
ACCESSION   DACDKL010000420 DACDKL010000000
VERSION     DACDKL010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.431"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="IV329_002949"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="IV329_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8942035.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT          207 a          124 c           92 g          121 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaa
//
LOCUS       DACDKL010000421          542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.432, whole genome shotgun sequence.
ACCESSION   DACDKL010000421 DACDKL010000000
VERSION     DACDKL010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.432"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..>542
                     /locus_tag="IV329_002950"
     CDS             7..>542
                     /locus_tag="IV329_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942036.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILS"
BASE COUNT          199 a           76 c          108 g          159 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tc
//
LOCUS       DACDKL010000422          538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.433, whole genome shotgun sequence.
ACCESSION   DACDKL010000422 DACDKL010000000
VERSION     DACDKL010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.433"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            87..>538
                     /locus_tag="IV329_002951"
     CDS             87..>538
                     /locus_tag="IV329_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8942037.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGR"
BASE COUNT          206 a           54 c          102 g          176 t
ORIGIN      
        1 gatattaaag ggatatttta ctttaaataa aaattaacaa ggtttataat ttgtagtagt
       61 taaagtattt aaagaggtgg gaacatgtga aggttcaaag gatagaagtg gagaataagc
      121 cgtatccatt gtatttatta ctagataaag aataccagct aatagaacca gtaatgaaat
      181 ttattaaata cttagataat actggtaagt ctcctaatac cattaaagca tactgctatc
      241 atttaaagtt gctgtacgag ttcatggaac agagaggtgt tattcttaat gatattaact
      301 ttgagttgtt agcagacttc gtaggttggt tgagatatcc ttcagcatca aatgtaattg
      361 atcttcagtc aaaaaaagcc ataagagaag aaacgacagt gaatacaatt ttaaatgtag
      421 ttatgagttt tcttgattat ttaagtagat taggagaatt taaatcaatt gatgtattta
      481 aacaagcaaa gggaagaaat ttcaaaggat ttttacatca tgttaataag ggtagata
//
LOCUS       DACDKL010000423          532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.435, whole genome shotgun sequence.
ACCESSION   DACDKL010000423 DACDKL010000000
VERSION     DACDKL010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.435"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>532
                     /locus_tag="IV329_002952"
     CDS             <1..>532
                     /locus_tag="IV329_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942038.1"
                     /translation="TFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYA
                     NKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQ
                     VASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYR
                     FTFSKQKQPRREVQHS"
BASE COUNT          199 a           94 c           66 g          173 t
ORIGIN      
        1 ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc catgctaaca
       61 aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa aattacaatc
      121 atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat aatgatcaat
      181 atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac aaacttacaa
      241 ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt tatcgttata
      301 aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct aagtttggca
      361 caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat cagtacacaa
      421 aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt ttactttatc
      481 gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct ca
//
LOCUS       DACDKL010000424          530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.436, whole genome shotgun sequence.
ACCESSION   DACDKL010000424 DACDKL010000000
VERSION     DACDKL010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.436"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..415
                     /locus_tag="IV329_002953"
     CDS             <1..415
                     /locus_tag="IV329_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8942039.1"
                     /translation="KVEYIYEFEGGKGITNQYRPNKFETFKEFYSLANTVTDDINKDG
                     MAALLLKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITK
                     LLYNMPAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            434..>530
                     /locus_tag="IV329_002954"
     CDS             434..>530
                     /locus_tag="IV329_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8942040.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNY"
BASE COUNT          193 a           75 c          110 g          152 t
ORIGIN      
        1 aaaagtagaa tatatttatg aatttgaagg tgggaagggg atcactaatc aataccgacc
       61 taacaagttc gaaacattta aagaattcta ttcattagca aatactgtaa cagacgatat
      121 taataaagac ggtatggcgg cgcttctttt aaaagggaca acttcagtta ttttttctat
      181 ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc agatattgag
      241 tgatccagtg atccgagaag ccaagcaaat tgctaagacg gattcttcag caaaaaaaat
      301 aacaaagttg ctttacaata tgccagctcc actattttta tctgctgtaa aagttggaag
      361 cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg aagcgagtat attagttaat
      421 ggaggaaaat ataatgaaaa gaattttgca ttttcaggga cgaatgggtt taggtggagc
      481 agagtctttc atgatgaatt tatatcgtaa aattgatcgt acaaactatc
//
LOCUS       DACDKL010000425          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.437, whole genome shotgun sequence.
ACCESSION   DACDKL010000425 DACDKL010000000
VERSION     DACDKL010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.437"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV329_002955"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IV329_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8942041.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDKL010000426          519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.438, whole genome shotgun sequence.
ACCESSION   DACDKL010000426 DACDKL010000000
VERSION     DACDKL010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.438"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..493
                     /locus_tag="IV329_002956"
     CDS             <1..493
                     /locus_tag="IV329_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8942042.1"
                     /translation="VVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIF
                     SKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAV
                     KITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKT
                     KRM"
BASE COUNT          192 a           91 c           86 g          150 t
ORIGIN      
        1 agttgttttt ttcgaactgt tgggtgaatc cagcagtagt ttttctacac tttgctttcc
       61 agaagccacg tccaatcatt ctagttcttt tatacaggaa aagaaattaa gagaaaaaat
      121 cgatacaatt ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc ttagtcaatt
      181 gatccaacac ttactacaaa cagaaacgaa agagcagtta ctcatccatt tgcagataac
      241 caaacagttt acaggaaatt atcatcttca aagccatttg cagcagttat ttcgcaaaga
      301 tgctgttaaa attaccgcat attcagaaca tgctgatttg atcattacag atcagcatga
      361 gaaaatttca gatgaaacgg agactttcta tctggataca acgcacaata cagtgttatt
      421 tggcaagtta ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg agaacaaaac
      481 aaagaggatg tgacaaaatt cttgtcacat cctcttatt
//
LOCUS       DACDKL010000427          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.439, whole genome shotgun sequence.
ACCESSION   DACDKL010000427 DACDKL010000000
VERSION     DACDKL010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.439"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>509
                     /locus_tag="IV329_002957"
     CDS             <1..>509
                     /locus_tag="IV329_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8942043.1"
                     /translation="TSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLD
                     THYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPW
                     ILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYD
                     NPVGVLTNN"
BASE COUNT          178 a           68 c           92 g          171 t
ORIGIN      
        1 tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg attatgaaat
       61 atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt ttcgaaaggt
      121 aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag ctgactaccc
      181 tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa atttttctgg
      241 gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt ttgaatttat
      301 tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt taaaaaatat
      361 caatttagca aatataaatt atagtgacga acttccttta tcccctttac attggctatt
      421 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
      481 taaccctgtg ggcgttctta ccaataatc
//
LOCUS       DACDKL010000428          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.440, whole genome shotgun sequence.
ACCESSION   DACDKL010000428 DACDKL010000000
VERSION     DACDKL010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.440"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDKL010000429          505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.441, whole genome shotgun sequence.
ACCESSION   DACDKL010000429 DACDKL010000000
VERSION     DACDKL010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.441"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..372
                     /locus_tag="IV329_002958"
     CDS             <1..372
                     /locus_tag="IV329_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8942044.1"
                     /translation="IHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGKPLKYQ
                     SVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTLNTYVH
                     LSDQDMKNEFNKYLERKEHKK"
BASE COUNT          185 a           68 c          107 g          145 t
ORIGIN      
        1 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
       61 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
      121 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
      181 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
      241 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
      301 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
      361 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
      421 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
      481 gaaaatgtcc acatccttct gccat
//
LOCUS       DACDKL010000430          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.443, whole genome shotgun sequence.
ACCESSION   DACDKL010000430 DACDKL010000000
VERSION     DACDKL010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.443"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..168)
                     /locus_tag="IV329_002959"
     CDS             complement(<1..168)
                     /locus_tag="IV329_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942045.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFY"
     gene            complement(152..493)
                     /locus_tag="IV329_002960"
                     /pseudo
     CDS             complement(152..493)
                     /locus_tag="IV329_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
BASE COUNT          176 a           89 c           32 g          196 t
ORIGIN      
        1 ataaaacatt gtttttccat caggtcttaa aagtgtatag caatatgcaa atcctataat
       61 tttattattt tctttagcta taaacccaaa tgaactggta tcattaagaa atccttttaa
      121 attatcaata tcaaaaacca tattatcatc aataaacaat agtttcaata ggataatatt
      181 cttcaactat atcttgatat tcttttgctt tctcaatatc tatatttcca tacattctta
      241 atatatcttc tccaaaattt gttcctattt cttcttcact atcttcaagt aagtatataa
      301 aatcacaata ttcatctata attccagaat ctccaaaatc aattattcca gttaatctat
      361 tattgccatc taacaataga tgattacaac taaaatcatt atggcataaa cactttttac
      421 cctcaaaaac tgttgttgca tttagtcttt ccataaaact ttctatataa tctttttcta
      481 tatcagttaa atc
//
LOCUS       DACDKL010000431          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.444, whole genome shotgun sequence.
ACCESSION   DACDKL010000431 DACDKL010000000
VERSION     DACDKL010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.444"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>491)
                     /locus_tag="IV329_002961"
     CDS             complement(<1..>491)
                     /locus_tag="IV329_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8942046.1"
                     /translation="IGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSK
                     NGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVS
                     GAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDF
                     TPS"
BASE COUNT          153 a           88 c           67 g          183 t
ORIGIN      
        1 agaaggtgta aaatcttgat tgttaatatg ttgagttgca atataagctg ttcttagagc
       61 attagaaata gaaaaatcaa cactacttct attaatggac gtaaaaaaag ttgtagcaca
      121 atccccaata gcatatatat cttttatatt tgtttgctgg tattcattag ttagatatgc
      181 tccactaaca tctctcaaga gttccttttc cccaattgga ctattagata gaaatcctat
      241 agcttgtacg accatatcca tagagtattg ggtaccactt gtagtgacta ttttttcaat
      301 cttttttctt cctaaatatt ttttaggact ttctgataga caaagatgta ttccattttt
      361 tgatagtatt tcttcaactt tatctgaaaa actacggtcg taataagtcg acaacactct
      421 atctgcaaca tcgaaaagat atacttcttt ccctcttttt cttatggctt cagcaatttc
      481 aactccaata t
//
LOCUS       DACDKL010000432          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.445, whole genome shotgun sequence.
ACCESSION   DACDKL010000432 DACDKL010000000
VERSION     DACDKL010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.445"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>491
                     /locus_tag="IV329_002962"
     CDS             <1..>491
                     /locus_tag="IV329_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942047.1"
                     /translation="DGRDKKYTLESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKM
                     YDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILD
                     PHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIE
                     HYF"
BASE COUNT          205 a           45 c           89 g          152 t
ORIGIN      
        1 gatggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
       61 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      121 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      181 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      241 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      301 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      361 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      421 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
      481 cattactttg a
//
LOCUS       DACDKL010000433          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.446, whole genome shotgun sequence.
ACCESSION   DACDKL010000433 DACDKL010000000
VERSION     DACDKL010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.446"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          154 a           78 c           82 g          173 t
ORIGIN      
        1 cagataataa cggaatatta tcatcgaaac agctaacaaa attgtcataa aaaacgagat
       61 ggttgatatt agaacttgtg tttttgttta ggtctagaga taaagagaga caatttccaa
      121 atatatgtaa tgaatacaaa aagacgtttg tcgcggatta ccgttacaaa cgtcttttat
      181 tgattttccg agatctcatc agtcgccatt tggaatggtt cactccttat ctagattcgt
      241 ttaatgctcg gactgcttgt tcgattgtaa taccaaacgc aacgttattt tcgttttgtg
      301 catcaatcgc cataaaacgg ttagaagttg tatcaaaagc tacgcggtat tgcatatttg
      361 tttttgtaaa cgtcataaaa ttcatctcct ttgtaatgta atttagttct tgaaagtttc
      421 ctttaaactt tcgaacttat accaagttta gcatcgtacg ttttttgatg caattaaaaa
      481 gcattta
//
LOCUS       DACDKL010000434          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.447, whole genome shotgun sequence.
ACCESSION   DACDKL010000434 DACDKL010000000
VERSION     DACDKL010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.447"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          166 a           53 c           85 g          181 t
ORIGIN      
        1 atttcgaaaa atgcctatga tttaaataat tatggtgctg ggatgaaggt ttataagttt
       61 gtcttgattt gtttagaaga aatcgttttt gcacacagtt gaattaggaa actactggag
      121 tggaaaataa agtgatttga cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa
      181 tgctttattt tgaattgcag aatttctttt agtacgcaat ttatggtctt aaaactgatt
      241 aaaaagtgat acattgattt taatacaatt tatgatacaa aaaatgacct cctttcttaa
      301 ataaaagagg tcattttgtt ttgtctcata atcgattgtt tttgatacag tagttatatt
      361 cagaaaaaat aaagactctt ctgagtttat aatcctttgg attatatacg gatacagatg
      421 tagacataaa acaagctcaa ttttcaaaaa tggcattact cacctttttg gtgaattatt
      481 tgtat
//
LOCUS       DACDKL010000435          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.448, whole genome shotgun sequence.
ACCESSION   DACDKL010000435 DACDKL010000000
VERSION     DACDKL010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.448"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..325
                     /locus_tag="IV329_002963"
     CDS             107..325
                     /locus_tag="IV329_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942048.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT          179 a           59 c          104 g          143 t
ORIGIN      
        1 gatatagaaa ttgatattct cgatgaatac aaacatgata ttttaattta ggtttatcat
       61 tggaaagttt agataaacaa agtactttta gaaaggaggg aaaaaaatga tttctgaacc
      121 tcaaatgaat aatatgtatg aaagcccaac tataatggga gctggtgaag ctgcgccaag
      181 agcttgttca gcaattatgg tgtgtgtgtt attagcatct gcaggagtag gtatccaagc
      241 aggagcggtg tatacttatg ctgtagttgc tacagtagct gtagctgcag ccgcagtttg
      301 gaaaacagta ggattagttg gttaggaatt aataggagac taactgtctc ctattgattc
      361 caatgattaa gctaaggagg aattttaaga aaataatgaa atacaataat ttaaaaccga
      421 aattacttaa tgagaacata gaatatataa gagaagaaaa ttatggatat ttaaccatag
      481 ttccc
//
LOCUS       DACDKL010000436          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.449, whole genome shotgun sequence.
ACCESSION   DACDKL010000436 DACDKL010000000
VERSION     DACDKL010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.449"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          178 a           70 c           92 g          145 t
ORIGIN      
        1 gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg
       61 cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagctact
      121 tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct
      181 tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg
      241 tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct cattatgcca
      301 ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt
      361 gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag
      421 atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt
      481 tctgt
//
LOCUS       DACDKL010000437          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.450, whole genome shotgun sequence.
ACCESSION   DACDKL010000437 DACDKL010000000
VERSION     DACDKL010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.450"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IV329_002964"
     CDS             complement(61..258)
                     /locus_tag="IV329_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8942049.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACDKL010000438          481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.451, whole genome shotgun sequence.
ACCESSION   DACDKL010000438 DACDKL010000000
VERSION     DACDKL010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.451"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(156..413)
                     /locus_tag="IV329_002965"
     CDS             complement(156..413)
                     /locus_tag="IV329_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8942050.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          140 a           94 c           73 g          174 t
ORIGIN      
        1 aaaaaataaa gaaacttatt cttgataaca agctacgtga cgtatatccc taacacaaaa
       61 atagccatat cctttggttt ggatacggct atttgatatt actcattcaa ttatcaaatc
      121 acagtaggtg tgaatctcat cgggatcggc aatatctact tctgattagg atcaaaggtg
      181 attttctcaa taaaggtgtg atacaaagct ttaacttgct tcttctcact ctggataaga
      241 aagcaatcca aactagtcaa tatctgttgg acatctttga cttgaatcgg ttccccttgt
      301 tgttgaaaga tccctaagat ttcattctct cgaatttgta actgtcttcg tttttcagtc
      361 aattcattta aacgatcttt taacatcgga aacaattcgg gactatcttc caaagcagct
      421 tcctgttttt gctctaatag ttagattttg cgtaccattt tcttctttta ttttccttcg
      481 a
//
LOCUS       DACDKL010000439          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.452, whole genome shotgun sequence.
ACCESSION   DACDKL010000439 DACDKL010000000
VERSION     DACDKL010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.452"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IV329_002966"
     CDS             complement(<1..430)
                     /locus_tag="IV329_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8942051.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          135 a           95 c           64 g          185 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcac
//
LOCUS       DACDKL010000440          466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.453, whole genome shotgun sequence.
ACCESSION   DACDKL010000440 DACDKL010000000
VERSION     DACDKL010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.453"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..405
                     /locus_tag="IV329_002967"
     CDS             <1..405
                     /locus_tag="IV329_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942052.1"
                     /translation="ISELYKSVGWTNYTKDTARLARAFEQSESLIKRNGEGKIIGIVR
                     WITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELLTDDTEKTKKF
                     YESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT          172 a           62 c          101 g          131 t
ORIGIN      
        1 atttctgaat tatataagag cgttggttgg acgaattaca caaaagacac tgcaaggtta
       61 gctagagcat ttgaacaatc tgaatctctg ataaagcgta atggggaagg gaagattatc
      121 ggaatcgttc gttggatcac tgattttgcg acgatcgcat ttattcaaga tatcctcatt
      181 catccgaggt atcagcgcca aggaataggc aaagtgcttt tgaatgaagc tttggagaag
      241 atcactagtt atggaccagt ccaaatcgaa ttattgacag atgatactga gaaaacaaaa
      301 aaattttatg aatcagtagg atttgttcaa gtaaaggaaa tgggtggtat ttcttatgtc
      361 atagatacta gaagaaaaaa ggtgcttttt ggtaatgaca aataacatat aataacgtac
      421 aaaagaaaaa attctagctt agttctttag aaaaacaaga ggatag
//
LOCUS       DACDKL010000441          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.454, whole genome shotgun sequence.
ACCESSION   DACDKL010000441 DACDKL010000000
VERSION     DACDKL010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.454"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>462
                     /locus_tag="IV329_002968"
     CDS             <1..>462
                     /locus_tag="IV329_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8942053.1"
                     /translation="FDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGG
                     IYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVM
                     VVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIA"
BASE COUNT          146 a           62 c           84 g          170 t
ORIGIN      
        1 ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa tcccacattc
       61 cttagcaaat atagttttag cacaattcga tagaattatg attaattctt atttaggtcc
      121 tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa gcgtaatatg
      181 gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta aaaataatga
      241 aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga ttacgatgat
      301 tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact attattctgg
      361 gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat atagtttacc
      421 agttaatgct gaattttatg agaaaaaaac atcgtttatt gc
//
LOCUS       DACDKL010000442          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.455, whole genome shotgun sequence.
ACCESSION   DACDKL010000442 DACDKL010000000
VERSION     DACDKL010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.455"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            266..>460
                     /locus_tag="IV329_002969"
     CDS             266..>460
                     /locus_tag="IV329_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8942054.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVT"
BASE COUNT          167 a           75 c           64 g          154 t
ORIGIN      
        1 atttattata cgtaaaaagc tcaagacatt ttcttagcca taaggacatg tttgatattt
       61 aagaagatat atgaattttc ttttctaagt ataaagataa ctatccatta tcaactaaaa
      121 aaacattttt gaaaataaac atcagcagtc ctatcaatgc atgtgagctt atagaagtta
      181 ttcgcagcgc agcataattg cattagcttc ttttcgtgtt ttttgcctct tttcccttta
      241 aatcttaatg gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa atgcccagtt
      301 gattcgattc tttgaaaagg tcattttgaa caccgctaat acttattata aaaaacaaga
      361 agaaattcaa aaacatgaac aacttgatca actcccacca tatttgttta ctaaagatac
      421 tataaaattt aaacaaccga ttaatgtttt aaatgtgact
//
LOCUS       DACDKL010000443          446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.456, whole genome shotgun sequence.
ACCESSION   DACDKL010000443 DACDKL010000000
VERSION     DACDKL010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.456"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          168 a           81 c           65 g          132 t
ORIGIN      
        1 tcccaacata gatggtgcgt ataatatcac atttaatgtc acgccaccat aacaataaca
       61 tgcaaaaagg gataaacagc atcgtgttct ccacgcattc tgttgtcaat ctgctctctc
      121 ctgtagcatt atctataata tataatgacc actgtccaat aacgtttgaa agcgggttca
      181 tccacatatt tcgattaagc aaggttttaa ataatatcaa agtaaaataa aacgcaagaa
      241 aaaagagtct gcgaaattta ctagatgctt taaaatatct caaccagatt aatacagaat
      301 cttttaagtt tttaggatct cgagagttat caactataaa tagataccag tacataaaca
      361 gatacgctgt taatacggca aataagaacg gctgatataa tgctgttaat acatttaccg
      421 ttatagcaga aataatatct atcatt
//
LOCUS       DACDKL010000444          443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.457, whole genome shotgun sequence.
ACCESSION   DACDKL010000444 DACDKL010000000
VERSION     DACDKL010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.457"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>443
                     /locus_tag="IV329_002970"
     CDS             <1..>443
                     /locus_tag="IV329_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8942055.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPG"
BASE COUNT          160 a           80 c           90 g          113 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcacaa tcatcactcc tgg
//
LOCUS       DACDKL010000445          439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.458, whole genome shotgun sequence.
ACCESSION   DACDKL010000445 DACDKL010000000
VERSION     DACDKL010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.458"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..401
                     /locus_tag="IV329_002971"
     CDS             <1..401
                     /locus_tag="IV329_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8942056.1"
                     /translation="KNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVS
                     GDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQI
                     TAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          160 a           49 c           79 g          151 t
ORIGIN      
        1 ctaaaaataa tttttcaaat caaataagtt tgaatgccta tagccgcggt atgggaggga
       61 taggcttgcc tggagattta tcctcagtat ctcgttttgt taaagcgact tttacgaagc
      121 tgaattctgt atctggagat tcagagtcag aaagtattag tcaatttttc catatcttag
      181 gttcagtaga acaacaaaaa ggtttgtgtg atgtaggtga tggaaaatat gaatatacaa
      241 tttattcttc ttgttgcaat gttgacaaag gaatctatta ttatcgaaca tatgaagaca
      301 gtcaaattac tgcaattgat atgaataaag aagacttaga tagtcataag ttaattagtt
      361 atccaattat agaaaaacaa caaattaaat atataaatta gttaatgtgt tgtgattgat
      421 tatttaatat agatataat
//
LOCUS       DACDKL010000446          435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.459, whole genome shotgun sequence.
ACCESSION   DACDKL010000446 DACDKL010000000
VERSION     DACDKL010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.459"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..172)
                     /locus_tag="IV329_002972"
     CDS             complement(<1..172)
                     /locus_tag="IV329_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8942057.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISI"
     gene            complement(191..346)
                     /locus_tag="IV329_002973"
                     /pseudo
     CDS             complement(191..346)
                     /locus_tag="IV329_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT          147 a           80 c           48 g          160 t
ORIGIN      
        1 ttattgatat tgaagaatta gaactttcat ataaagcctt ttctatttca ttgcttgatt
       61 tcattattgt taaacaagaa tacaagcaag aaagaactat agttaatatg ataaaaatta
      121 ttaaagttct atttttcttt cttgtaatat atgctatagc attttttatc actttcctaa
      181 ctactcctct ctattttctt actccataac taattgcatc cactggacaa gcatttttac
      241 atcgtccaca tctgatacac tccagatgat tgcaattctc aactgaatct ttattcattt
      301 ggcaaacttt tttacatttt ccacaattaa tacacttatc cttatcctga atcggctaca
      361 ctagactaga cagaaaaaat aaggtgtgta gaataataaa aaacacactg gaggattttt
      421 catgccaaga cgtca
//
LOCUS       DACDKL010000447          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.460, whole genome shotgun sequence.
ACCESSION   DACDKL010000447 DACDKL010000000
VERSION     DACDKL010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.460"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV329_002974"
     CDS             complement(<1..132)
                     /locus_tag="IV329_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8942058.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          150 a           85 c           83 g          114 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aa
//
LOCUS       DACDKL010000448          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.461, whole genome shotgun sequence.
ACCESSION   DACDKL010000448 DACDKL010000000
VERSION     DACDKL010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.461"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..107
                     /locus_tag="IV329_002975"
                     /pseudo
     CDS             1..107
                     /locus_tag="IV329_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554414.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            165..>426
                     /locus_tag="IV329_002976"
     CDS             165..>426
                     /locus_tag="IV329_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HAP8942059.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILK"
BASE COUNT          179 a           58 c           71 g          118 t
ORIGIN      
        1 ttactttatc tcattcataa tgaaaaaaat ggaaaggaga taaaagtatg ggtacttttt
       61 ctatatttgt tattaataaa gttcgttatc aaccaaatca aaattaattg gttataatga
      121 acgcttaatg tcagttcatt ataaccagta aggagaaggt tataatgaac cagaaaaacc
      181 ctaaagacac gcaaaatttt attacttcta aaaagcatgt aaaagaaata ttgaatcaca
      241 cgaatatcag taaacaagac aacgtaatag aaatcggatc aggaaaagga cattttacca
      301 aagagctagt caaaatgagt cgatcagtta ctgctataga aattgatgga ggcttatgtc
      361 aagtgactaa agaagcggta aacccctctg agaatataaa agtgattcaa acggatattc
      421 taaaat
//
LOCUS       DACDKL010000449          425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.462, whole genome shotgun sequence.
ACCESSION   DACDKL010000449 DACDKL010000000
VERSION     DACDKL010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.462"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>425)
                     /locus_tag="IV329_002977"
     CDS             complement(<1..>425)
                     /locus_tag="IV329_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8942060.1"
                     /translation="SIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDY
                     QKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFF
                     LSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHIL"
BASE COUNT          164 a           78 c           74 g          109 t
ORIGIN      
        1 taaaatatgt gtcagcatat cactaggagc cataccactt aattcttttc ccagtgggac
       61 tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg cacttaaaaa
      121 aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt ggtcccaaaa
      181 ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga cagaaagtaa
      241 atgagtatac actactagcg tcatgccaat agacaaccag taccattttt ggtaatctct
      301 aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag cgacagcttc
      361 tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg tataaataat
      421 cgaaa
//
LOCUS       DACDKL010000450          424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.463, whole genome shotgun sequence.
ACCESSION   DACDKL010000450 DACDKL010000000
VERSION     DACDKL010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.463"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(170..415)
                     /locus_tag="IV329_002978"
     CDS             complement(170..415)
                     /locus_tag="IV329_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP8942061.1"
                     /translation="MIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          130 a           83 c           58 g          153 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cact
//
LOCUS       DACDKL010000451          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.464, whole genome shotgun sequence.
ACCESSION   DACDKL010000451 DACDKL010000000
VERSION     DACDKL010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.464"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..127
                     /locus_tag="IV329_002979"
     CDS             <1..127
                     /locus_tag="IV329_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8942062.1"
                     /translation="ESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            128..>415
                     /locus_tag="IV329_002980"
     CDS             128..>415
                     /locus_tag="IV329_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8942063.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFL"
BASE COUNT          156 a           50 c           86 g          123 t
ORIGIN      
        1 tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa acgtagtaac
       61 aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa tagctgagga
      121 gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg ggtttaaaaa
      181 taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag caatgataga
      241 tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata cggacagtaa
      301 tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc
      361 tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat tctta
//
LOCUS       DACDKL010000452          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.465, whole genome shotgun sequence.
ACCESSION   DACDKL010000452 DACDKL010000000
VERSION     DACDKL010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.465"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IV329_002981"
     CDS             complement(<1..339)
                     /locus_tag="IV329_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8942064.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDKL010000453          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.466, whole genome shotgun sequence.
ACCESSION   DACDKL010000453 DACDKL010000000
VERSION     DACDKL010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.466"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IV329_002982"
     CDS             <1..>404
                     /locus_tag="IV329_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942065.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACDKL010000454          397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.467, whole genome shotgun sequence.
ACCESSION   DACDKL010000454 DACDKL010000000
VERSION     DACDKL010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.467"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>397
                     /locus_tag="IV329_002983"
     CDS             <1..>397
                     /locus_tag="IV329_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8942066.1"
                     /translation="THNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAI
                     ELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKR
                     NLTIIGEKILIEWEGNPDSLFSWNEETSSM"
BASE COUNT          139 a           51 c           73 g          134 t
ORIGIN      
        1 acgcataata aaaaaatttc ttatcaatac catgtaggac agtatttacc cgactggcat
       61 ccttgggaag attatcgcaa tttttttgtt tcaaacaaaa aaactaatgg gtgtagagaa
      121 atattcgcta ttgaattacc ttggattata gatacatttg ggaaagttaa atcgtttact
      181 gtaatgaccc agaaattatc caatcttgat attgattatt cggacagttt cactcttatt
      241 attgagcatg aaactggtat agttggaact ttaagtgtag atgttgtttc aagagtagct
      301 aaaagaaatt taactataat tggtgaaaag attttaatag aatgggaagg taacccggat
      361 tctctatttt catggaatga agaaacttct agtatga
//
LOCUS       DACDKL010000455          393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.469, whole genome shotgun sequence.
ACCESSION   DACDKL010000455 DACDKL010000000
VERSION     DACDKL010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.469"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..300
                     /locus_tag="IV329_002984"
     CDS             <1..300
                     /locus_tag="IV329_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8942067.1"
                     /translation="DQEVKSGQREDGTIYAVYSFARKIGQALAGGVGGWTLSIIGYDS
                     LAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENYNLLNQKHN"
     gene            311..>393
                     /locus_tag="IV329_002985"
     CDS             311..>393
                     /locus_tag="IV329_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8942068.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGP"
BASE COUNT          131 a           52 c           79 g          131 t
ORIGIN      
        1 gatcaagaag tgaaatctgg ccaaagagaa gatggaacta tttatgcagt atattctttt
       61 gcccgaaaaa ttggacaagc tttagctggc ggtgtcggag gttggactct ttctataata
      121 gggtatgatt cgttagcaaa agtacaaagt ggagaagttt tacaaaagct gtataactct
      181 tcaacgttga ttccagcagt ttgtttcttt gttgtaggat tgattttatt tttcgtatat
      241 ccattatcta aagaaaaagt attagaaaat tataatttgc taaatcaaaa acataactag
      301 gagaaaaaac atgaacagtt ggatattttg tattgattca gatggttgtg tcatggatac
      361 aatgacttat aaacacaatc tctttttcgg gcc
//
LOCUS       DACDKL010000456          386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.471, whole genome shotgun sequence.
ACCESSION   DACDKL010000456 DACDKL010000000
VERSION     DACDKL010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.471"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..>386)
                     /locus_tag="IV329_002986"
     CDS             complement(37..>386)
                     /locus_tag="IV329_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942069.1"
                     /translation="IPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAG
                     KPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQ
                     IDDFPEEIIDRIE"
BASE COUNT          106 a           76 c           67 g          137 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgt
//
LOCUS       DACDKL010000457          384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.472, whole genome shotgun sequence.
ACCESSION   DACDKL010000457 DACDKL010000000
VERSION     DACDKL010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.472"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          141 a           72 c           49 g          122 t
ORIGIN      
        1 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
       61 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      121 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      181 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      241 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      301 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      361 ttaaatgttt cttacaatca aaaa
//
LOCUS       DACDKL010000458          384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.473, whole genome shotgun sequence.
ACCESSION   DACDKL010000458 DACDKL010000000
VERSION     DACDKL010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.473"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..363)
                     /locus_tag="IV329_002987"
     CDS             complement(<1..363)
                     /locus_tag="IV329_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942070.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQ"
BASE COUNT          125 a           74 c           69 g          116 t
ORIGIN      
        1 ctgtagttta taaaaaaacg gcgaatgaag atgtgttttc atatgaccaa aatataataa
       61 ttctgaaatt tctaaaggtg tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca
      121 cttcaattca tcgaatccac gcctgttacg agaagaggaa aagaaacgct gaatattttc
      181 tgtaccacgg attactttca gccctgtatg ggattcatat tctccagcag aagcagatgg
      241 atcaagcaag agcaagtttt gaggaaaatg cacgatactg tttgcaaagt cagtagcagt
      301 cagccctttt gttaaaacag cattacttgt ggtatcaata tgtacataaa ctgactcagt
      361 catcatgttc ctcctcttat ttcc
//
LOCUS       DACDKL010000459          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.475, whole genome shotgun sequence.
ACCESSION   DACDKL010000459 DACDKL010000000
VERSION     DACDKL010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.475"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            90..284
                     /locus_tag="IV329_002988"
     CDS             90..284
                     /locus_tag="IV329_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942071.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          167 a           50 c           67 g           97 t
ORIGIN      
        1 ttatttatca taaagatata gatacataat cattttcact tacatatatt tgaatattaa
       61 ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt agaagtaaaa acaaaaggcg
      121 tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata tcctttactt ggttcatgga
      181 aagaagctgc tcctttttcc aaaccatgtg ccataaaaat aaagaacgag ctagaaaaag
      241 aactgacttg tggtaaagct gttgtagata tcatcgaaaa gtaaatccaa tgtatagaaa
      301 ggaagatcac acatgaaaag cgacgtacaa attgctaaga ttatagaatt agaagaagaa
      361 gacgcagtgt tgacaattga a
//
LOCUS       DACDKL010000460          377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.476, whole genome shotgun sequence.
ACCESSION   DACDKL010000460 DACDKL010000000
VERSION     DACDKL010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.476"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>377)
                     /locus_tag="IV329_002989"
     CDS             complement(<1..>377)
                     /locus_tag="IV329_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942072.1"
                     /translation="MIERQVVARRKVKYYLLIASALAYSAVIHGSRSVLVYQIIVLGC
                     YILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGSAESRFASVW
                     FFIPKFLQSPLFGTGILTGEEFT"
BASE COUNT          146 a           75 c           57 g           99 t
ORIGIN      
        1 cgtaaattcc tcaccagtaa gaataccagt accgaacaaa ggcgattgca aaaatttagg
       61 aataaaaaac caaactgaag caaatcgaga ttcagcagat cctgcatatt cacctgtact
      121 gtttgtgaaa gtttcaacaa atgttgagaa catggtcgaa ttaataaata tgactacaag
      181 caacccagct aaaacccaca ttgcaatttt tcttttcgaa gaacctttct taaaaaggat
      241 atagcaacca agtacaataa tttgatagac aaggacagat ctagaaccat gaataacagc
      301 agaatatgcc agtgcgctag caatcagtaa ataatatttt acttttcttc tagctacaac
      361 ttgtcgctca atcatat
//
LOCUS       DACDKL010000461          371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.477, whole genome shotgun sequence.
ACCESSION   DACDKL010000461 DACDKL010000000
VERSION     DACDKL010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.477"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>371
                     /locus_tag="IV329_002990"
     CDS             <1..>371
                     /locus_tag="IV329_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942073.1"
                     /translation="SIKKGSLTLITGSSGSGKSTLLKLMNGLIPRLYDGKLVGEILFE
                     NNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTSELVFSMENFGFTKEHMDKRINELA
                     ELLDLSNILDKNIFSLSSGER"
BASE COUNT          142 a           49 c           66 g          114 t
ORIGIN      
        1 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
       61 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
      121 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
      181 ttggctacgt aagtcaagat cctagaggac aattctttac aacaaataca acgagtgagt
      241 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
      301 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
      361 gtggtgaacg g
//
LOCUS       DACDKL010000462          370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.478, whole genome shotgun sequence.
ACCESSION   DACDKL010000462 DACDKL010000000
VERSION     DACDKL010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.478"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          120 a           61 c           78 g          111 t
ORIGIN      
        1 tgctcctcaa ctgaaatcaa ttaaagaact agaatttgag aatgcactgg taatttgtat
       61 gacatgtact gatgaagtat ttcgaaaaca tttttaaagg ttgagatgta tatagattta
      121 accaatcatt tatctctctc ggaaaaggaa tattaactaa ccagcgggtt atcaaaaata
      181 ggtaggaaaa ctgtacttaa tggcttattt cgcgctgatt atgagaatat cagtctgttt
      241 gattaatttt tagggttcaa aatacagaga caaactccag gtcgggtttc gccacaagcg
      301 aattacccga tctggagttt tgtctattgc aggaaggcgt agccggactg caggaataaa
      361 gcccccggtt
//
LOCUS       DACDKL010000463          366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.479, whole genome shotgun sequence.
ACCESSION   DACDKL010000463 DACDKL010000000
VERSION     DACDKL010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.479"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..252)
                     /locus_tag="IV329_002991"
     CDS             complement(34..252)
                     /locus_tag="IV329_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8942074.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          128 a           66 c           64 g          108 t
ORIGIN      
        1 cttgtctttt gaataattag cagtcaatcc ttattatgat tgcctggatc tgtagtaggg
       61 ctcatgatag aaatgatctt catgaattct gcgccgctca gattgacggt acttcttttc
      121 atcccacaac atgaaccaca aagtaaataa cggaacaaac acaagtaaag ctggacgtat
      181 gccgaaatta ccaataataa tacctaaaaa caaaacagtt aatactaagc caagacgtct
      241 cattgatttc atgaattctc acctctttaa tttatgcgaa caattgttcg tatatttagt
      301 atacgaatat ttgttcggtt tgtaaagagg gaagtgataa aaatttgaaa gaagaagaac
      361 ccgggc
//
LOCUS       DACDKL010000464          361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.480, whole genome shotgun sequence.
ACCESSION   DACDKL010000464 DACDKL010000000
VERSION     DACDKL010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.480"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..>361
                     /locus_tag="IV329_002992"
     CDS             75..>361
                     /locus_tag="IV329_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8942075.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
BASE COUNT          127 a           58 c           74 g          102 t
ORIGIN      
        1 atttttgaaa acttgccata ctttttattt gttttatttt ccagtatact atacgaaagg
       61 aaggtggcta gaccatgaaa gttgtacaaa caaaagatac aatgagcaat atttacttag
      121 atgctgtacg gatcaggcat caagtttttg tagtcgaaca gggtgtgcct ttgtctcgag
      181 aaatagacaa agatgaagcc cattgtatcc acttcgtcct gtactcagat aaaaaagaac
      241 cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa aatgaaatta cagcggatgg
      301 cgattctttc tgaatacaga caccaagggt taggaaaaat attgattgaa gaagcagaaa
      361 a
//
LOCUS       DACDKL010000465          341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.denovo.481, whole genome shotgun sequence.
ACCESSION   DACDKL010000465 DACDKL010000000
VERSION     DACDKL010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.denovo.481"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            141..332
                     /locus_tag="IV329_002993"
     CDS             141..332
                     /locus_tag="IV329_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8942076.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          128 a           50 c           54 g          109 t
ORIGIN      
        1 atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata
       61 actaattttg tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc
      121 catgggaaga aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg
      181 gaaatgaaga agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat
      241 cctggagaac tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc
      301 tagctatctc gaaattcgaa attaaagaat aactcaacta a
//
LOCUS       DACDKL010000466         5985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.140, whole genome shotgun sequence.
ACCESSION   DACDKL010000466 DACDKL010000000
VERSION     DACDKL010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.140"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IV329_002994"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IV329_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IV329_002995"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IV329_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8942077.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IV329_002996"
     CDS             1851..3005
                     /locus_tag="IV329_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8942078.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="IV329_002997"
     CDS             3220..4188
                     /locus_tag="IV329_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8942079.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="IV329_002998"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="IV329_002998"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8942080.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="IV329_002999"
     CDS             5218..5826
                     /locus_tag="IV329_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8942081.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1162 c         1347 g         1665 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaac cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     5941 ggcagagggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DACDKL010000467         5985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.141, whole genome shotgun sequence.
ACCESSION   DACDKL010000467 DACDKL010000000
VERSION     DACDKL010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.141"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IV329_003000"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IV329_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IV329_003001"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IV329_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8942082.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IV329_003002"
     CDS             1851..3005
                     /locus_tag="IV329_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8942083.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="IV329_003003"
     CDS             3220..4188
                     /locus_tag="IV329_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8942084.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="IV329_003004"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="IV329_003004"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8942085.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="IV329_003005"
     CDS             5218..5826
                     /locus_tag="IV329_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8942086.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1347 g         1666 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     5941 ggcagagggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DACDKL010000468         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.211, whole genome shotgun sequence.
ACCESSION   DACDKL010000468 DACDKL010000000
VERSION     DACDKL010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.211"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..1489
                     /locus_tag="IV329_003006"
     CDS             101..1489
                     /locus_tag="IV329_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8942087.1"
                     /translation="MEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKG
                     FRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEI
                     EAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKP
                     RFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLK
                     YYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELN
                     KLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAH
                     IFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
                     VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVK
                     ERLEQIIGSLTG"
     gene            1496..1873
                     /locus_tag="IV329_003007"
     CDS             1496..1873
                     /locus_tag="IV329_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8942088.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            2027..2809
                     /locus_tag="IV329_003008"
     CDS             2027..2809
                     /locus_tag="IV329_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP8942089.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2935..>3376)
                     /locus_tag="IV329_003009"
     CDS             complement(2935..>3376)
                     /locus_tag="IV329_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP8942090.1"
                     /translation="IYGNIPYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRA
                     LGLLLMVEMDIKMLKKVPPLYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWV
                     NREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         1237 a          487 c          654 g          998 t
ORIGIN      
        1 gggtgttaga attaccacta ctaattataa gattactgct aatcaagaaa aaacacctgt
       61 aaaagctaat tcctactatg ttactaatct aaaacaattt atggagtttt atgagaactt
      121 ttattttgat ggagaggagt gggataaaga cgtttgggat agacgtaact tacctttgcc
      181 agatgataag gttaacccaa cacaatatga atatacaatt aactttaaag ggtttcggaa
      241 tacatatttt aaacaacttg taaaaagata ttgtaagttg agattgaaca tggatagctt
      301 ttcctatgta agtgatattg cccaaagact taaagagttc tttaattttc tggacatgaa
      361 atttaaacaa gttcagagag tacaccaatt aacgagagtg gaaattgaag catatttaag
      421 tgaactaaac atgatgggaa taaaacctag tacaataact gggaggatct ctatattgga
      481 aggactattt agtacccttc ttaggctaga atgggatgat gttccttcca aaatattaat
      541 ttattctgag gactatccga aaataccaag agcaaaacca cgctttatag atgaattcgt
      601 cctagagcaa ttgaacagtc atcttgataa attacccgaa tatatagcta cgatgactat
      661 gattgttcaa gaatgtggaa tgaggataag tgaattgtgc accttgaaaa aaggctgtct
      721 attagaggac aaagatggag atttcttttt aaagtattat caatggaaaa tgaaaaagga
      781 gcatatagtt ccaatatcta aagaggtagc tttacttatt aaagttcggg aagataaagt
      841 ttcagaggaa tttccagata gtgaatacct ctttccaaga aaagatggat cgccattaaa
      901 acaagaaaca tttagaggtg agttaaataa attagcttat gagcaaaata tagtggataa
      961 atcaggtgag atttatagat tccatgccca tgcctttcgc catacagtag gaacaagaat
     1021 gattaacaac gggatgcccc agcatattgt gcagaaattt ttggggcatg aaagcccaga
     1081 aatgacaagc agatacgctc atatctttga tgaaactcta aaaaatgaat ttactaaatt
     1141 tcaggaaaaa ctggttacca ataatggaga tgtgcttgat ctagatgaag ataatgaagt
     1201 cgatgatgta gagcttcaat ggttcaagaa aaatataaat gcacaagtgc ttccaaatgg
     1261 ttattgtaga ttgccagtag tagcaggtgg ttgtccacat gcgaatgcat gcttagattg
     1321 cactcacttc tgtaccagta agcaattctt accacagcac gaagaacagt tagagcgtac
     1381 agaagagtta ttagccatag ctaaggataa acaatggcaa agacaagtag agactaatag
     1441 ccgtgttaaa gagcgtttag aacaaatcat tggaagtttg acggggtaat tatcaatgga
     1501 taaacaagtt agaaatacaa cagaaattgt acgtttggcg aagcagaaat caaaaaagac
     1561 aagggaaaaa gtagacaaag cgatttctaa attttcgatt gaaggtaaag ttattaattt
     1621 taattcaata gcaaaggaag ctaatgtttc taaatcatgg ctttataagg aacacgatat
     1681 taggcaaaga atcgaatccc ttcgtgagcg tcaaataaca gcaaatgtag tctcaaaacc
     1741 caagaaaagt tctcgttcgg aggaaatcct tattaaaacc ttaaaaagaa gagtaatgga
     1801 attagaaaaa gaaaataaaa aattacagaa ccaaattcaa aaattatatg gagatctgta
     1861 taataaagaa taattattaa tctgtagaca aattgtgaaa ggatgtactt aaacgctaac
     1921 ggtcagcttt attgaacagt catttaggta tatgtccaat ctagggtaag taaattgagt
     1981 atcaatataa actttatatg aacataatca gttacgaggt gaaatcatga gcaatttgat
     2041 taacggaaaa ataccaaatc aagcgattca aacattaaaa atcgtaaaag atttatttgg
     2101 aagttcaata gttggagtat atctatttgg ttcagcagta aatggtggtt tacgcattaa
     2161 cagcgatgta gatgttctag tcgtcgtgaa tcatagttta cctcaattaa ctcgaaaaaa
     2221 actaacagaa agactaatga ctatatcagg aaagattgga aatacggatt ctgttagacc
     2281 acttgaagtt acggttataa ataggagtga agttgtccct tggcaatatc ctccaaaaag
     2341 agaatttata tacggtgagt ggctcagggg tgaatttgag aatggacaaa ttcaggaacc
     2401 aagctatgat cctgatttgg ctattgtttt agcacaagca agaaagaata gtatttctct
     2461 atttggtcct gattcttcaa gtatacttgt ctccgtacct ttgacagata ttcgaagagc
     2521 aattaaggat tctttgccag aactaattga ggggataaaa ggtgatgagc gtaatgtaat
     2581 tttaacccta gctcgaatgt ggcaaacagt gactactggt gaaattacct cgaaagatgt
     2641 cgctgcagaa tgggctatac ctcttttacc taaagagcat gtaactttac tggatatagc
     2701 tagaaaaggc tatcggggag agtgtgatga taagtgggaa ggactatatt caaaggtgaa
     2761 agcactcgtt aagtatatga aaaattctat agaaacttct ctcaattagg ctaattttat
     2821 tgcaataaca ggtgcttact tttaaaacta ctgatttatt gataaatatt gaacaatttt
     2881 tgggaagaat aaagcgtcct cttgtgaaat tagagaacgc tttattactt taatttagtg
     2941 aaacaatttg taactattga aaatagaaag aaattgttcc ttcgatagtt tattaatatt
     3001 agtgacattt gcatgcttca aagcctgtcg gaattggttt ttagtgaaaa gaacacgata
     3061 ttcacggttt acccacttat aaacaaaaga tcgatacttt ttgtagtcct tctttgaaat
     3121 caatggttga tgtcgttcaa gaacaatcaa tacagagtct acacttggct taggatgaaa
     3181 atatagtggt ggtacttttt tgagcatttt tatatccatc tccaccatta atagtaaacc
     3241 caaagctcgt tgcagatttt gcaatctttt cgcaaatccc ttctcaacga taagatagct
     3301 atatttagcc tgactttcaa aggtaattct tttgacaata tccgtactga tgttataagg
     3361 aatattacca tatatc
//
LOCUS       DACDKL010000469         2947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.236, whole genome shotgun sequence.
ACCESSION   DACDKL010000469 DACDKL010000000
VERSION     DACDKL010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.236"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            45..1166
                     /locus_tag="IV329_003010"
     CDS             45..1166
                     /locus_tag="IV329_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8942091.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1424..2926
                     /locus_tag="IV329_003011"
     CDS             1424..2926
                     /locus_tag="IV329_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8942092.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
BASE COUNT         1025 a          508 c          653 g          761 t
ORIGIN      
        1 aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg
       61 caattgattc atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg
      121 gctgggaaga tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata
      181 ctgcagaagc aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc
      241 tcttattccg tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg
      301 cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa
      361 ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag
      421 aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat
      481 tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta
      541 ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg
      601 ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac
      661 aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc
      721 agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag
      781 gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag
      841 tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag
      901 aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg
      961 caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag
     1021 gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag
     1081 aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca
     1141 gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt
     1201 tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc
     1261 tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct
     1321 tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca
     1381 gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat
     1441 aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct
     1501 aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac
     1561 tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga
     1621 atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa
     1681 tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga
     1741 tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct
     1801 actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca
     1861 tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt
     1921 catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat
     1981 cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa
     2041 acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct
     2101 aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa
     2161 gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc
     2221 tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat
     2281 cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag
     2341 ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa
     2401 tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg
     2461 tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa
     2521 ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta
     2581 tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc
     2641 atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga
     2701 gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc
     2761 tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt
     2821 agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt
     2881 cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca
     2941 agaacaa
//
LOCUS       DACDKL010000470         2091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.279, whole genome shotgun sequence.
ACCESSION   DACDKL010000470 DACDKL010000000
VERSION     DACDKL010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.279"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..545
                     /locus_tag="IV329_003012"
     CDS             <1..545
                     /locus_tag="IV329_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8942093.1"
                     /translation="EKRRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPG
                     AVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQS
                     KEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSET
                     EAQLEEKMARWEYLSEWVED"
     gene            557..1504
                     /locus_tag="IV329_003013"
     CDS             557..1504
                     /locus_tag="IV329_003013"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8942094.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1524..2051
                     /locus_tag="IV329_003014"
     CDS             1524..2051
                     /locus_tag="IV329_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8942095.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
BASE COUNT          708 a          357 c          472 g          554 t
ORIGIN      
        1 gagaaaaacg ccggttattt ttgttgaaac tgctgattca gcaacctaat gttttattat
       61 tggatgaacc gaccaatgat ttggacatag caacattgac gattttggaa gattatttta
      121 gaagttttcc aggtgctgtg atcactgttt cccatgaccg ttatttcttg gacaaggtag
      181 cagataagtt attagtattc caaggaaacg gaaaacaaga actttattac ggaaacatgt
      241 ccagttattt actgaagcaa aaagagaccc agcagcctgc tgaaaaagcg aaaccaaaaa
      301 tacagagcaa ggaacctgct gaaaagaaga aattatctta catggaacaa aaagaatggg
      361 aaacgattga agatgagatt gcagaacttg aagaaaaaat ctcattgctc caagaagaga
      421 tgaatcatca aggagataat ttcactcgat tacaagaatt gcaaaacgac gtatctgaaa
      481 cagaagcaca acttgaagaa aaaatggcac ggtgggaata cttgagtgaa tgggtagaag
      541 attaggagga aaaaggatgg aacaagcgta tcttgactta ggaagaaaat tgttggaaga
      601 agggcatcgt aaagaagaca gaacaggcac aggcacatta agcttattcg gataccaaat
      661 gcggtttgat ctgcaaaaag ggtttccttt gttaacaacg aaacgagtac cttttggatt
      721 gatcaaaagc gaattgcttt ggtttttaaa aggagatacg aatatccgct atctattaca
      781 aaataaaaac catatttggg atgaatgggc atttgaacgc tacatcaaaa gtccagacta
      841 taatggacca gatatgactg attttggcca tagatgtttg acagatcctg aattcaatga
      901 agaatataaa aagcagagca agcttttttg cgagaagatc ctaaccgatg attcctttgc
      961 tgaaacatac ggcgatcttg gacacatcta cggctatcaa tggcgtcact gggaaacaaa
     1021 agacggtggc tttatcgatc aaatcaaaca agtgatcgaa gcaatcaaaa agactcctga
     1081 ttctagaaga ttgatcgttt ctgcatggaa tccggaagat gttcctagca tggctttgcc
     1141 tccttgtcat acgatgtttc aattttatgt tcaagaggga cggctaagct gccaattgta
     1201 tcaacgcagt ggggatgtgt ttctaggcgt tccgttcaac attgcaagtt atgcgttgct
     1261 gactcaccta attgcacatg aaacaggttt agcggttggc gaatttgttc acacattagg
     1321 agatgctcat ctttatatca atcatatcga acaaatgaaa gaacaattga gccgagaaat
     1381 acgcgcattt ccgactttag ttttgaatca ggaaaagcag tcagtatttg attttgagat
     1441 ggaagatatc caattagaag gatatgaccc acatccggcg atcaaagctc ctatcgctgt
     1501 ttagaatcag aaaggatgag aaaatgttag ctgcaatttg ggcacaggat gaaaacggat
     1561 tgattggaaa agaagaccgt cttccttggc gtttgccaaa tgatctgaaa ttcttcaaac
     1621 aaatgacaga agcaaataca ctagtgatgg gacgaaaaac attcgaaggg atgggtagcc
     1681 gtccgttgcc gaatcgtcag acgattgttt tgacgagaga ttcttcctat aaagcagaag
     1741 gcgtgcatgt catgcatagt gtggaagacg tgcttgctct ggaaaaagaa acagacggta
     1801 tctttttcat cgctggaggg tcagctgttt atcaagaatt tctgccattt tgtacgatat
     1861 tataccgtac agtcatccat catgtattcg atggagatgc ttattttcca cctgttgact
     1921 ggtctgattg gtcactgatc aatactagtc aaggagaaat ggatgaaaaa aatcgttatc
     1981 cgcatcaatt tgaaacgtat cagcgtaatg agaatcctgt aaaaagcggg acaggcgaat
     2041 tgacgatata aaaaagctat ggatagacta gctgttattt agagctgtcc c
//
LOCUS       DACDKL010000471         2010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.288, whole genome shotgun sequence.
ACCESSION   DACDKL010000471 DACDKL010000000
VERSION     DACDKL010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.288"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..320)
                     /locus_tag="IV329_003015"
     CDS             complement(<1..320)
                     /locus_tag="IV329_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8942096.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFP"
     gene            682..1722
                     /locus_tag="IV329_003016"
     CDS             682..1722
                     /locus_tag="IV329_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8942097.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1820..2008
                     /locus_tag="IV329_003017"
     CDS             1820..2008
                     /locus_tag="IV329_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8942098.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
BASE COUNT          681 a          331 c          335 g          663 t
ORIGIN      
        1 gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta
       61 cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc
      121 ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg
      181 ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc
      241 ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca
      301 gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc
      361 ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt
      421 tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt
      481 caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag
      541 tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca
      601 tatatacgcc tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa
      661 gaaaaaataa aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat
      721 agagcttatt ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt
      781 cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta
      841 caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg
      901 gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt
      961 atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag
     1021 tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa
     1081 aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt
     1141 ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt
     1201 ggatacttaa atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa
     1261 caaggattat atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac
     1321 tatagccctg ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa
     1381 gcctttagaa aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca
     1441 ccacaggaat atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta
     1501 aatactcaac tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt
     1561 caagattatc gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag
     1621 ctaaaaggat atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt
     1681 cggatagata aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat
     1741 ataaatatgg tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag
     1801 tggaaaggag gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa
     1861 taaaacaaga ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc
     1921 caaatgtaag aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga
     1981 gccagacgcc aagacgcaga gccgataatt
//
LOCUS       DACDKL010000472         1620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.297, whole genome shotgun sequence.
ACCESSION   DACDKL010000472 DACDKL010000000
VERSION     DACDKL010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.297"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..1503
                     /gene="msr"
                     /locus_tag="IV329_003018"
     CDS             25..1503
                     /gene="msr"
                     /locus_tag="IV329_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8942099.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1550..>1620
                     /locus_tag="IV329_003019"
     CDS             1550..>1620
                     /locus_tag="IV329_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8942100.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGM"
BASE COUNT          578 a          273 c          347 g          422 t
ORIGIN      
        1 acatgaaata ggagtgtaaa acatatggaa aatttagcag taaatataac aaatctacaa
       61 gtcagctttg gcaaccagct agaactatct attgattctc ttcgtgtcta tcagcaagat
      121 cgtataggga tcataggaga aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc
      181 ggtgaacttt ttcccgatca tgggaaaatc caaacagaga tcaccttcaa ctacctgcct
      241 caattaacct atctcgctga agcaaaggac ctaaatttgg aattagccag tcatttccag
      301 ttaagactgg aagaaacttc agagcggaaa tggagcggag gggaagaacg aaagatcgag
      361 ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc tagacgagcc aacaacccat
      421 ctagatagaa aaagtattga tcgactgatt gaagagcttc gttattatta tggtacgctg
      481 gtttttgtta gtcatgatcg ctattttcta gatgagctag catcgaaaat ctgggaagta
      541 aaagacggag aaatccgaga gttttcgggg aattatagtg cctatctcac tcaaaaggaa
      601 ttggagaaaa agactcagct acgagaagca gagtcgatca tgaaagagaa aaaacgattg
      661 gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa
      721 aagaaaaagc aacaaatcag actggatcgg ttgtcttcct ctaaacaaaa agacagtgta
      781 caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa gactccaaaa aataggagaa
      841 acaaccaagc cgcagcagat gaaacaaatc cgttttccag taccaaaatc tcttgaactc
      901 cacagccgct atcccatcat gggacaaaat gtccaattgg aaagaagtgg gagaacatta
      961 ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat
     1021 ggttcaggta agacaacctt attagaacat atccgcaaac aaggagaagg aatccttctc
     1081 tctccgaaag taagctttca agtatatcag caaaagggtt atcaaatgac atctgaagaa
     1141 tccatcattc gttttgtcat gagacaaaca gagttttcag aatcgcttgt ccgtagtttg
     1201 ctgaatcact tagggtttgc tcaggaaact ttgacgaaac cgttatgtac attaagtggg
     1261 ggagaagcga cccgtctgac gattgctttg ctttttacta agccaagtaa tgtgttgctg
     1321 ttagatgaac cgactaattt tattgatatg gcaacgatcg aagctttaga gaagctaatg
     1381 caaatatatc cgggaacgat tttgtttact tctcatgatt cttactttgt cgagcgtacg
     1441 gctgatgaag tttatgaaat aaaagggcag aaaataaaaa aagtacttac gagaaatttt
     1501 taaatgtgct acgatgattt tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa
     1561 taaaaaagaa ggaataattt ttacaacaat gatgtgtttt ttgatggtct tggggatgag
//
LOCUS       DACDKL010000473         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.320, whole genome shotgun sequence.
ACCESSION   DACDKL010000473 DACDKL010000000
VERSION     DACDKL010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.320"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..84)
                     /locus_tag="IV329_003020"
     CDS             complement(<1..84)
                     /locus_tag="IV329_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8942101.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYG"
     gene            637..1185
                     /gene="aac(6')"
                     /locus_tag="IV329_003021"
     CDS             637..1185
                     /gene="aac(6')"
                     /locus_tag="IV329_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8942102.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          468 a          204 c          265 g          415 t
ORIGIN      
        1 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
       61 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
      121 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
      181 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
      241 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
      301 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
      361 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
      421 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
      481 tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga
      541 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
      601 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
      661 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
      721 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
      781 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
      841 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
      901 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
      961 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
     1021 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
     1081 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
     1141 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
     1201 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt
     1261 tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg
     1321 tgaaaaagtc gcttagtttc gagtagtaag aa
//
LOCUS       DACDKL010000474         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.321, whole genome shotgun sequence.
ACCESSION   DACDKL010000474 DACDKL010000000
VERSION     DACDKL010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.321"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..298
                     /locus_tag="IV329_003022"
                     /pseudo
     CDS             47..298
                     /locus_tag="IV329_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="IV329_003023"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="IV329_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8942103.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DACDKL010000475         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.342, whole genome shotgun sequence.
ACCESSION   DACDKL010000475 DACDKL010000000
VERSION     DACDKL010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.342"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            259..>1196
                     /gene="vanY"
                     /locus_tag="IV329_003024"
     CDS             259..>1196
                     /gene="vanY"
                     /locus_tag="IV329_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8942104.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DACDKL010000476          919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.388, whole genome shotgun sequence.
ACCESSION   DACDKL010000476 DACDKL010000000
VERSION     DACDKL010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.388"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            17..754
                     /gene="erm(B)"
                     /locus_tag="IV329_003025"
     CDS             17..754
                     /gene="erm(B)"
                     /locus_tag="IV329_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8942105.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          349 a          157 c          141 g          272 t
ORIGIN      
        1 cttcaggagt gataacatga acaaaaatat aaaatattct caaaactttt taacgagtga
       61 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      121 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      181 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac
      241 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      301 taaaattgtt gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      361 tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      421 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      481 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      541 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      601 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      661 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
      721 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg
      781 gaaagttaca cgttactaaa gggaatgtag ataaattatt aggtataggg cacctctaat
      841 aactaccaat taattcacct caaaatgaaa ttagaaaaaa acagcgaaat ttttaaatct
      901 atttcttatc gatacaaat
//
LOCUS       DACDKL010000477          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20827_2016
            SAMN12640399-rid9887723.guided.434, whole genome shotgun sequence.
ACCESSION   DACDKL010000477 DACDKL010000000
VERSION     DACDKL010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640399
            Sequence Read Archive: SRR10040894
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742838.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742838.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:34:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,026
            CDSs (total)                      :: 2,964
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640399-rid9887723.guided.434"
                     /isolate="E20827_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..>536
                     /gene="aac(6')-I"
                     /locus_tag="IV329_003026"
     CDS             5..>536
                     /gene="aac(6')-I"
                     /locus_tag="IV329_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HAP8942106.1"
                     /translation="MCIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLESLKKHYT
                     EPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWS
                     KGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEG
                     KKEDCYLMEYRYDDNAT"
BASE COUNT          222 a           46 c           97 g          171 t
ORIGIN      
        1 tgaaatatgt ataagaactt taatagatga tgattttcct ttgatgttaa aatggttaac
       61 tgatgaaaga gtattagaat tttatgatgg tagagataaa aaatatacat tagaatcatt
      121 aaaaaaacat tatacagagc cttgggaaga tgaagttttt agagtaatta ttgaatataa
      181 caatgttcct attggatatg gacaaatata taaaatgtat gatgagttat atactgatta
      241 tcattatcca aaaactgatg agatagtcta tggtatggat caatttatag gagagccaaa
      301 ttattggagt aaaggaattg gtacaagata tattaaattg atttttgaat ttttgaaaaa
      361 agaaagaaat gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag
      421 ggcataccaa aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga
      481 gggcaaaaaa gaagattgtt atttaatgga atatagatat gatgataatg ccacaa
//
